LOCUS       DAVKDZ010000001        65428 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000001 DAVKDZ010000000
VERSION     DAVKDZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 65428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65428
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.001"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            683..943
                     /locus_tag="VWZ30_000001"
     CDS             683..943
                     /locus_tag="VWZ30_000001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333831.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHEYCGIAY
                     ALFAYSKVLEPSMFYNDLHTFHTELKKLLEKVTSNTENLGNL"
     gene            989..1270
                     /locus_tag="VWZ30_000002"
     CDS             989..1270
                     /locus_tag="VWZ30_000002"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333832.1"
                     /translation="MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQ
                     NKLLMKKIQQVILACSERDDHGLLMFQGDSGKADLFDFGIGSMGYIGVY"
     gene            1313..2545
                     /locus_tag="VWZ30_000003"
     CDS             1313..2545
                     /locus_tag="VWZ30_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter permease"
                     /protein_id="HEU9333833.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLRILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLTLITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLASIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(2663..3661)
                     /gene="cbpJ"
                     /locus_tag="VWZ30_000004"
     CDS             complement(2663..3661)
                     /gene="cbpJ"
                     /locus_tag="VWZ30_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEU9333834.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLATNTVFADNSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(3680..4642)
                     /gene="cbpC"
                     /locus_tag="VWZ30_000005"
     CDS             complement(3680..4642)
                     /gene="cbpC"
                     /locus_tag="VWZ30_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEU9333835.1"
                     /translation="MKILKKMMQVLLAVFFFGLLATNTVFANTTGGRFIDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDGGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     EGHWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTINIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWYQDGSTWYYLDPSN
                     GDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(4743..5429)
                     /locus_tag="VWZ30_000006"
     CDS             complement(4743..5429)
                     /locus_tag="VWZ30_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9333836.1"
                     /translation="MGKRILLLEKERNLAYFLSLELQKEQYRVDRVEEGQKALSMALQ
                     TDYDLILLNAHLGDMTAQDFADKLSRTKPASVIMVVDHREELQDQIETIQRFAVSYIY
                     KPVIIENLVARISAIFRGRDFIDQHCSQMKVPTSYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQ"
     gene            complement(5441..6865)
                     /gene="gndA"
                     /locus_tag="VWZ30_000007"
     CDS             complement(5441..6865)
                     /gene="gndA"
                     /locus_tag="VWZ30_000007"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU9333837.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAD
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFKFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            complement(6941..8311)
                     /gene="mapZ"
                     /locus_tag="VWZ30_000008"
     CDS             complement(6941..8311)
                     /gene="mapZ"
                     /locus_tag="VWZ30_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEU9333838.1"
                     /translation="MSKKRRNRHKKEGQEPQFEFDEAKELTVGQAIRKNEEVEVGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEELVETQSLDDLIQEMREAVEESEAYSKEV
                     PSSEDILLPLPLDDEEQGLDPLLLEDENPTEMTEEVEEEQNLSRLEQEDSEKKSKKGF
                     VLTALALVSVIICVSAYYVYRQVNRSTKEIEASQSTTANQSDVDDFNTLYDTFYTDGN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQASSNTTS
                     ETKPSSSNEARSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAWDFADGVLE
                     QILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGYFVGNGAGH
                     ADDLDY"
     gene            complement(8324..9481)
                     /locus_tag="VWZ30_000009"
     CDS             complement(8324..9481)
                     /locus_tag="VWZ30_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU9333839.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPKGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKSLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKSNA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            complement(9566..9946)
                     /gene="rnpB"
                     /locus_tag="VWZ30_000010"
     ncRNA           complement(9566..9946)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VWZ30_000010"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(9967..10296)
                     /gene="gpsB"
                     /locus_tag="VWZ30_000011"
     CDS             complement(9967..10296)
                     /gene="gpsB"
                     /locus_tag="VWZ30_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEU9333840.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            complement(10366..10893)
                     /locus_tag="VWZ30_000012"
     CDS             complement(10366..10893)
                     /locus_tag="VWZ30_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEU9333841.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKMIKKAIRKDLESMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYRQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            10960..11556
                     /gene="recU"
                     /locus_tag="VWZ30_000013"
     CDS             10960..11556
                     /gene="recU"
                     /locus_tag="VWZ30_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEU9333842.1"
                     /translation="MVNYPHKLSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVRVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEAKETKQ
                     KGAIPMKNFHPHQIQHMEQVLAQQGICFILLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLGYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            11553..13706
                     /locus_tag="VWZ30_000014"
     CDS             11553..13706
                     /locus_tag="VWZ30_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU9333843.1"
                     /translation="MKKPTILRLLKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTNLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEQETGYNLLTTGMDVYTNVDQEAQKHLWDIYNSDQYVSYPDDDLQVASTVV
                     DVSNGKVIAQLGARHQASNVSFGTNQAVETNRDWGSAMKPITDYAPAIEYGVYDSTAT
                     MVNDIPYNYPGTSTPVYNWDRAYFGNITLQYALQQSRNVTAVETLNKVGLDRAKTFLN
                     GLGIDYPSMHYANAISSNTTESNKQYGASSEKMAAAYAAFANGGIYHKPMYINKVVFS
                     DGSEKEFSDVGTRAMKETTAYMMTEMMKTVLAYGTGRGAYLPWLAQAGKTGTSNYTDD
                     EIEKHIKNTGYVAPDEMFVGYTRKYSMAVWTGYSNRLTPIVGDGFLVAAKVYRSMITY
                     LSEDTHPEDWTMPDGLFRNGEFVFKNGARPIWTEPSTQQSSTAESSTTQASTTVGQQP
                     NNAPATDPNQGQAVQPTQPVQPTPQNPQVQQQPQQ"
     gene            complement(13871..15850)
                     /locus_tag="VWZ30_000015"
     CDS             complement(13871..15850)
                     /locus_tag="VWZ30_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9333844.1"
                     /translation="MKTRKVLALAGVTLLAAGVLAACSGGSGAQGEKTFAFTYETHPD
                     NLNYLTTGKAATSEITSNVIDGLLENDKYGNLIPSMAEDWSVSKDGLTYTYKIRQDAK
                     WYTSEGEEYAPVKAQDFVTGLKYATDKKAEALYLVQDSIKGLDAYAKGEITDFAQVGI
                     KALDDQTVQYTLNKPETFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGPY
                     LLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARLY
                     PTSASFAELEKSMKDNIVYTQQDSTTYLVGTNIDRQSYKHTSKTSDEQKTSTKKALLN
                     KDFRQAIAFGFDRTAYASQLNGETGASKILRNLFVPPTFVQADGKNFGDIVKEKLVTY
                     GDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVQFPIHLDMPVDQTATTKVQR
                     VQSMKQSLEATLGTDNVVIDIQQLQKDELLNVTLFAENAAGEDWDLSDNVGWGPDFAD
                     PSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDETTDVAKRYD
                     KYAAAQAWLTDSALIIPTTSKTGRPILSKMVPFTIPFALSGNKGTSDPVLYKYLELQD
                     KAVTADEYQKAQEKWMKEKAESNKKAQEELAKHVK"
     gene            complement(16087..17187)
                     /gene="glf"
                     /locus_tag="VWZ30_000016"
     CDS             complement(16087..17187)
                     /gene="glf"
                     /locus_tag="VWZ30_000016"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEU9333845.1"
                     /translation="MYDYLIVGAGLSGAIFAYEATKRGKKVKVIDKRDHIGGNIYCEN
                     VEGVNVHKYGAHIFHTSNKKVWDYVNQFAEFNNYINSPVANYKGSLYNLPFNMNTFYA
                     MWETKTPQEVKDKIAEQTAHMKDVEPKNLEEQAIKLIGPDIYEKLIKGYTEKQWGRSA
                     TDLPPFIIKRLPVRLTFDNNYFNDRYQGIPIGGYNVIIENMLKDVEVELGLDFFANRQ
                     ELEASAEKVVFTGMIDQYFDYKHGELEYRSLRFEHEVLNEENYQGNAVVNYTEREIPY
                     TRIIEHKHFEYGTQAKTVITREYPADWKRGDEPYYPINDERNNAIFAKYQEEAATNDK
                     VIFCGRLADYKYYDMHVVIERALEVVAEELGQ"
     gene            complement(17520..19478)
                     /locus_tag="VWZ30_000017"
     CDS             complement(17520..19478)
                     /locus_tag="VWZ30_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9333846.1"
                     /translation="MTTKKRILSAGLTFAAALLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYADNTAIITNLVDGLLENDNHGNLVPSLAEDWSVSSDGLTYTYKLRKDAKWF
                     TADGEEYAPVKAQDFVTGIKYAVDNKSQAIDLIQNSIKGLNDYITGADSDFSKVGVKA
                     IDDQTVEYTLTRPEPYWNSKTTNSILFPVNEEFLNSKGKDFGTLSPDSILYSGPYLLK
                     DFTSKSSIEYVKNPHYYDHDKVSIEHVKLAYFDGSDQELTIRNFESGAYSIAGVYPNS
                     SNFAKTKEKYKDNIVYSLQDKTSWYFNFNVNRKAYNHTSKTTDEQKKSTETAVLNKNF
                     RQAVNFALDRTAYSAQSNGEEAASKTLRNTLVPPTFVQVGDKTFGEVVASKLVNYGTE
                     WSGINLADAQDAYFNKEKAQAKFVEAKKELASQGVTFPIHLDVAVDQTSKNAVTGMNS
                     VKQTLESVLGADNVVIDVQQLSTDDFNNVAFLAPTAADRDYDLNFDGWVGDYQDPSTY
                     LNPFNAEDGFYLKIFGLDAKEDKEKITSLGLDTYTKMLKDADSENKDVAKRYEKYAEA
                     QAWMIDNSLIMSAMSSGGTASVTKVTPFTRGYSLVGIKGDGNNYKYMKLQKDTVTTKQ
                     FEEAKTKWEQESKKAIEKAQKEAEKHVK"
     gene            complement(19705..21672)
                     /locus_tag="VWZ30_000018"
     CDS             complement(19705..21672)
                     /locus_tag="VWZ30_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9333847.1"
                     /translation="MQSKKWLIGAGVTLSTAILLTACGKSEKNADAPKTFSYVYAVDP
                     SSLDYSLTSKSSTSDVIGNVVDGLLENDKYGNLIPSLAEDWSVSKDGLTYTYKLRKGV
                     KWYTSEGEEYAEVKAQDFVTGLKHAADGKSDGLSLVEKSIKGLEAYVSGETNDFSTVG
                     VKALDDYTVEYTLNNPESFWNSKVTTATMLPVNEEFLNATGKDYGAPTPSSILYNGPY
                     FLKSLISKSVIEYEKNPNYWDKDNVKIDNVKLTFYDGSDQESLIRSFTQGAYTTARLF
                     PASSNFESTKKEYGDKIVYSPQEATSYYLTVNVNRQSYNKTAKTDESQKTSTKEALLN
                     KNFRQALNFAFNRHAYTAQLNGEEGADKIIRNSLVPDNYVQVAGKTFGQLAQDELLKY
                     GEQWKDVTLTDGKDTIYNPTKAKSAFEKAKSELQAKGVSFPIHLDVPVEQTDVVAVQQ
                     TNSLKQSIEETLGTENVIIDVLQMTDNEKESITSQAKVPTQKDYDLNGTGWGPDYQDP
                     ATYLNILDAKKGSALKHLGITKGKDPEVVAKVGLDEYKKLLDDAASETSNLDKRYEKY
                     AKAQAWVTDSSLLIPVASSGGSPMVSRTVPFTKAYSQVGIKGDPFIFKGMELQNDIVT
                     TKEYDAAFKKWQKEKLESNAQYQKDLEKHIK"
     gene            complement(21942..23552)
                     /locus_tag="VWZ30_000019"
     CDS             complement(21942..23552)
                     /locus_tag="VWZ30_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU9333848.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKNLLTVGETWGATPAIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVSMEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEEINVQEALANPDSIFYTYKKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGTVKSV
                     LIENTTAQEVFEKQILAPWDAFCVELTD"
     gene            23705..25189
                     /locus_tag="VWZ30_000020"
     CDS             23705..25189
                     /locus_tag="VWZ30_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEU9333849.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGESPYA
                     SPTDMGVNMVGFAITDNEAAIEASKQEIIRRYYQTVLDFKAEKVSESAVKKIELLMND
                     LGITPSDRKVAVVARQKAEETGGPALALELPSGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPELVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            25284..25766
                     /locus_tag="VWZ30_000021"
     CDS             25284..25766
                     /locus_tag="VWZ30_000021"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEU9333850.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAEIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(25937..28042)
                     /locus_tag="VWZ30_000022"
     CDS             complement(25937..28042)
                     /locus_tag="VWZ30_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9333851.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLAGNAEVDGKMPQQPSDMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QEASEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEVGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAEDTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     EAEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGATDIERLKDMGHRLQTKVIGQDKAVEAVAKAIRRNRAGFDEGNRPIGSFLF
                     VGPTGVGKTELAKQLALDMFGTKDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKEIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            complement(28699..29679)
                     /locus_tag="VWZ30_000023"
     CDS             complement(28699..29679)
                     /locus_tag="VWZ30_000023"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU9333852.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLIAAVVVSFLLALFSKQLTNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVVFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGIDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFVFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKMTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            complement(29681..31933)
                     /locus_tag="VWZ30_000024"
     CDS             complement(29681..31933)
                     /locus_tag="VWZ30_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEU9333853.1"
                     /translation="MKWTKKVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAVFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELETAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQQTVDGKDVYTTISSTLQSFMETQ
                     MDAFLEKVKGKYMTATLVSAKTGEILATTQRPTFNADTKEGITEDFVWRDILYQSNYE
                     PGSAMKVMTLASSIDNNTFPSGEYFNSSEFKIADATTRDWDVNDGLTTGGMMTFLQGF
                     AHSSNVGMSLLEQKMGDATWLDYLKRFKFGVPTRFGLTDEYAGQLPADNIVSIAQSSF
                     GQGISVTQTQMLRAFTAIANDGVMLEPKFISAIYDTNNQSVRKSQKEIVGNPVSKEAA
                     STTRNHMILVGTDPLYGTMYNHYTGKPIITVPGQNVAVKSGTAQIADEKNGGYLVGST
                     NYIFSVVTMNPAENPDFILYVTVQQPEHYSGIQLGEFATPILERASAMKESLNLQSPA
                     KNLDKVTTESSYAMPSIKDISPGELAEALRRNIVQPIVVGTGTKIKETSVEEGTNLAP
                     NQQVLLLSDKVEEIPDMYGWKKETAETFAKWLDIELEFEGSGSVVQKQDVRTNTAIKN
                     IKKIKLTLGD"
     gene            complement(31937..32254)
                     /gene="ftsL"
                     /locus_tag="VWZ30_000025"
     CDS             complement(31937..32254)
                     /gene="ftsL"
                     /locus_tag="VWZ30_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU9333854.1"
                     /translation="MAERMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(32266..33216)
                     /gene="rsmH"
                     /locus_tag="VWZ30_000026"
     CDS             complement(32266..33216)
                     /gene="rsmH"
                     /locus_tag="VWZ30_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU9333855.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKSAKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMEMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            33383..33577
                     /locus_tag="VWZ30_000027"
     CDS             33383..33577
                     /locus_tag="VWZ30_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9333856.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            33593..34135
                     /locus_tag="VWZ30_000028"
     CDS             33593..34135
                     /locus_tag="VWZ30_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEU9333857.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADRIGNQVFIVLFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITAIDPDMLSEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIGEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKDQDED"
     gene            34146..34688
                     /locus_tag="VWZ30_000029"
     CDS             34146..34688
                     /locus_tag="VWZ30_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEU9333858.1"
                     /translation="MKKETFTEKLIKRTYGISDPLDEYKRREADRIGNQVFIFLFYLM
                     IFGNLIPLLLAYKYPQLVALVYPPLILVIALIVAGYLTYQMQKTGITTIEPDMLNEKE
                     SKQLHYPGLKAGIVYGLMSFFTTPLLHILLGESQDYLQSLLAFKNIFSSILQSVFFGV
                     VIQILISRRIAKARKNQDND"
     gene            34699..34941
                     /locus_tag="VWZ30_000030"
     CDS             34699..34941
                     /locus_tag="VWZ30_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333859.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVILFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            34952..35275
                     /locus_tag="VWZ30_000031"
     CDS             34952..35275
                     /locus_tag="VWZ30_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333860.1"
                     /translation="MKSLLTLLTYHLLFSSLLIFIIVSGNLIREIFLVLVFVPALNKV
                     LTYLKIDSPKTRTLNLALYFMILSFPQLMLVSGNWNYYLLLTIASLSSIIYLYYLYQF
                     VKENQ"
     gene            35302..35880
                     /locus_tag="VWZ30_000032"
     CDS             35302..35880
                     /locus_tag="VWZ30_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEU9333861.1"
                     /translation="MKKEDLTTRLLRNLFHIQGPFDECRQEIIYKACARSMVQIVYSS
                     FFLFLFYLLFGRFIEAVRYAMPYVYSGLIFFITLKTQRVVKELHLEKDDKSEIILKTY
                     SKAQIKFRSWIVFIGLQIGLFTLLIFHKVFVQQMSLSDFVKLLMQFDKSVPFLMYGLI
                     IGSIFGTLTYGFLSLQEEKIPKNTKQKEKSKQ"
     gene            complement(36151..37152)
                     /locus_tag="VWZ30_000033"
     CDS             complement(36151..37152)
                     /locus_tag="VWZ30_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9333862.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIKKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSTQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSRLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHARLT
                     IEDKHTNLEQIKEFLQKEINPDEKTLVFVPNCWALPPVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPAFEEGQQATKILIDQIEGLNQEERQQVLDCSVSWKESTF"
     gene            complement(37212..39113)
                     /locus_tag="VWZ30_000034"
     CDS             complement(37212..39113)
                     /locus_tag="VWZ30_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEU9333863.1"
                     /translation="MKEERRQFFEKIDGDQYRDYILSHCSQDYEKVKRSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWNQVFEDDPEWAYMLNRQEYLLQFMIGYLVEGDKGYI
                     QKCKFFLFDWIEQVREFRPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEEELENIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPILATYHFFSDKMDLEEAYHFASKELKQQ
                     IETQILEDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSFQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAMVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPKFRSFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDT
                     GRYSYREVDERYLLKSAWSHSTCIVDGKAPERIVGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYLSAEPDLPYLHRRKILMLAEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLKLWSEVDFDLEATIISPKYNELEKSSKLIKHQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDGHQVENALAFEVKNDETDKLILLLSEDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            complement(39135..39428)
                     /gene="yajC"
                     /locus_tag="VWZ30_000035"
     CDS             complement(39135..39428)
                     /gene="yajC"
                     /locus_tag="VWZ30_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9333864.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRKEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            complement(39428..40246)
                     /locus_tag="VWZ30_000036"
     CDS             complement(39428..40246)
                     /locus_tag="VWZ30_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9333865.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEVFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADTKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKILA"
     gene            complement(40233..41017)
                     /locus_tag="VWZ30_000037"
                     /pseudo
     CDS             complement(40233..41017)
                     /locus_tag="VWZ30_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
     gene            complement(41032..41523)
                     /locus_tag="VWZ30_000038"
     CDS             complement(41032..41523)
                     /locus_tag="VWZ30_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9333866.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNAPGKEQVTRSIFLGGEDKAALKELNQTYQVTFNTKTTPTGNDGAVEVNIM
                     DYI"
     gene            complement(41534..42724)
                     /locus_tag="VWZ30_000039"
     CDS             complement(41534..42724)
                     /locus_tag="VWZ30_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEU9333867.1"
                     /translation="MIKKVTIEEIKSPERFLEVPLLTKEEVDQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDEFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREAALKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYVSANNVIRDDASSFHT
                     FYFDPETGQPLKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            complement(42736..43170)
                     /locus_tag="VWZ30_000040"
     CDS             complement(42736..43170)
                     /locus_tag="VWZ30_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9333868.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            43442..44257
                     /locus_tag="VWZ30_000041"
     CDS             43442..44257
                     /locus_tag="VWZ30_000041"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEU9333869.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEVGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASDFVNGHILYVDGGILAYIGKQPE"
     gene            44276..44917
                     /locus_tag="VWZ30_000042"
     CDS             44276..44917
                     /locus_tag="VWZ30_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU9333870.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     NPLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVVPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            44948..45949
                     /locus_tag="VWZ30_000043"
     CDS             44948..45949
                     /locus_tag="VWZ30_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU9333871.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGYRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRTILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFVRFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            45959..46588
                     /locus_tag="VWZ30_000044"
     CDS             45959..46588
                     /locus_tag="VWZ30_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU9333872.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNPYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSALSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(46966..47351)
                     /gene="tnpA"
                     /locus_tag="VWZ30_000045"
                     /pseudo
     CDS             complement(46966..47351)
                     /gene="tnpA"
                     /locus_tag="VWZ30_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596946.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(47732..50935)
                     /locus_tag="VWZ30_000046"
                     /pseudo
     CDS             complement(47732..50935)
                     /locus_tag="VWZ30_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
     gene            51158..51634
                     /locus_tag="VWZ30_000047"
     CDS             51158..51634
                     /locus_tag="VWZ30_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU9333873.1"
                     /translation="MTSLYDFSFLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLQDQKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            complement(51856..54069)
                     /locus_tag="VWZ30_000048"
     CDS             complement(51856..54069)
                     /locus_tag="VWZ30_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEU9333874.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGNFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHCYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(54471..54647)
                     /locus_tag="VWZ30_000049"
     CDS             complement(54471..54647)
                     /locus_tag="VWZ30_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333875.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            54794..55216
                     /locus_tag="VWZ30_000050"
     CDS             54794..55216
                     /locus_tag="VWZ30_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9333876.1"
                     /translation="MRFIVETFPIFSISNDLLQLLTSWKKQQKYELAKFGIISNPEQF
                     IFTYIDTKGNINKPLHSDYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLE
                     AISEALIHSDTGTTQIYVNTSNVVPMAVGEFALQSLKQ"
     gene            complement(55478..55870)
                     /gene="rpsI"
                     /locus_tag="VWZ30_000051"
     CDS             complement(55478..55870)
                     /gene="rpsI"
                     /locus_tag="VWZ30_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU9333877.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(55890..56336)
                     /gene="rplM"
                     /locus_tag="VWZ30_000052"
     CDS             complement(55890..56336)
                     /gene="rplM"
                     /locus_tag="VWZ30_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU9333878.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            56693..57010
                     /locus_tag="VWZ30_000053"
     CDS             56693..57010
                     /locus_tag="VWZ30_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEU9333879.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPLYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(57055..57867)
                     /gene="folB"
                     /locus_tag="VWZ30_000054"
     CDS             complement(57055..57867)
                     /gene="folB"
                     /locus_tag="VWZ30_000054"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEU9333880.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFETYPLVQEIKLELKKPWA
                     PVHLPLDTCSVTIHRRKQRAFIALGSNMGDKQANLEQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVIEVETWLPAPVLLETLLAIESEMGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIASHFIHPTLKQPIRNLYNALKK"
     gene            complement(57910..58464)
                     /gene="folE"
                     /locus_tag="VWZ30_000055"
     CDS             complement(57910..58464)
                     /gene="folE"
                     /locus_tag="VWZ30_000055"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU9333881.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(58445..59767)
                     /locus_tag="VWZ30_000056"
     CDS             complement(58445..59767)
                     /locus_tag="VWZ30_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9333882.1"
                     /translation="MKEFENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVIHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESIPEARLEALMADY
                     RSLLEGESATNLQGTTEFEIITAIAYDYFALEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGTPLVTGRIAPEALAVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALGDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(59769..60746)
                     /gene="folP"
                     /locus_tag="VWZ30_000057"
     CDS             complement(59769..60746)
                     /gene="folP"
                     /locus_tag="VWZ30_000057"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU9333883.1"
                     /translation="MVKSLEGVTDMSSKANHAKTAICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGRSSYVEIEEEIQRVVPVIKAIRKESNVLISID
                     TWKSQVAEAALAAGANLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVIARPQHPSSL
                     IFPHFGFGQAFTEKELADFETLPIEDLMETFFDRALARAEEAGIAQENILLDPGIGFG
                     LTKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTA
                     SAHVTSIAVRQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(60840..61547)
                     /locus_tag="VWZ30_000058"
     CDS             complement(60840..61547)
                     /locus_tag="VWZ30_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9333884.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTVVFALLHQPSNLPSLLIYGGMSIVLSWTAYKTQRLEMSILLHMIVNGVAF
                     CLLALVVILSRTLGISV"
     gene            complement(61600..63018)
                     /locus_tag="VWZ30_000059"
     CDS             complement(61600..63018)
                     /locus_tag="VWZ30_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU9333885.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            63208..64020
                     /locus_tag="VWZ30_000060"
     CDS             63208..64020
                     /locus_tag="VWZ30_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9333886.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            64359..65378
                     /gene="adhP"
                     /locus_tag="VWZ30_000061"
     CDS             64359..65378
                     /gene="adhP"
                     /locus_tag="VWZ30_000061"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEU9333887.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIATADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLTLAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT        19120 a        14486 c        11657 g        20165 t
ORIGIN      
        1 atttgtcctg ttattatttc attttactat aatatttgac aagttatcct aaaagtatta
       61 ttatgttgtt gtgttataga ttgattgaat ctacctaaag gatcctattc aattactaga
      121 actatcacat actcaaggtc agctcacaga tgagcaacta ttttggttac aatgtctact
      181 aaatttaagt caaacaaata attagtcaaa attaaaaaaa tagaggaaca taaatatgat
      241 tacaaaacag aatgtaatag tgttctacga tttttactag ataaaactgt aaattctgaa
      301 ggaaggatca cttcttcaac agaatttgga aatttcgtaa gtaatttatc attccaacac
      361 ggaatagctg gactactgtt tcatctaaat aaattgtacc ccccagaact ggattctaaa
      421 atactctcta tcatcaagaa ggcagtgaca attagaacga cacacacata tgaatatcaa
      481 tactcactgc tatttggtga tgcaggctat ctatagttac tcctacattt attttctatc
      541 agtaaaaatc aatactatct acaattagca aacgtcaccg ctaagaaatt aatagagaat
      601 tatgatactc tagaggaaat agactttgca ttgggaaaat ctgatgtcct attatcatta
      661 ataaaatact atcattttac caatgacaat actcttaaaa ctttcatcca caatagtata
      721 tggggaaatt tatcattatt tcctacaaag agatacagcc aaagaaagca ttttagacta
      781 tagctttgct catgaatatt gtggaattgc atatgcttta tttgcctatt ctaaagtctt
      841 agaaccttct atgttctata atgatctcca tacattccat actgaattaa aaaaattatt
      901 agaaaaagtt acttctaata ctgaaaattt aggaaattta taactttctt ggtgcgaagg
      961 aatttccgga ataatcttat atctttgtat gtacgattgt gacggaaaca aagatattat
     1021 tagtaaatat caagaatttg tttttaacca tcatctaaaa atgatgacag gatattgcca
     1081 cggaataact agcttactac aaaccactgt ctacaatcaa aacaaattac tgatgaaaaa
     1141 aatccaacag gtaattttag catgttctga acgagatgat cacggtttac tgatgtttca
     1201 aggagatagt ggtaaagcag atttgtttga cttcggaata ggaagcatgg ggtatattgg
     1261 tgtctattaa ataataaatt cccattcgat gtgcagacat aaggagaaat gtatgaaatt
     1321 attttggaca aacaacatat atagacagtt gctgctaaac agctgttttt catcattcgg
     1381 cgacagtatt ttctacctcg ccattatcaa ttatgtggct cagtacaatt tcgctccgct
     1441 agcgatttta ctgatttcca tttcagagat ggttccccta ctatcgcaac tctttctcag
     1501 gattctagga gattttcaag aaaatagagt caaacacgca ctctgtattg ccaaaatcaa
     1561 aatcctgctc tacgctattt tgacagtatt tctcgtcttg tcgccctttt cattagtttc
     1621 agtcattatg attgtcatca tcaacctcat ctctgacacc ttgagctacc tgtctgccta
     1681 catgatgaac gccctctaca tcagtgtaat taaggacgac ctgcatgatg ccatggggtt
     1741 caggcagtct ctgatgaggg ttgtccgcat tgtcgccaat ctggctggcg cattccttat
     1801 caatgttata agtattcaaa ctatttccct tatcaacact ctgacttttg tcattgcctt
     1861 tttgggcctg tatgttattc gacatacctt gtatgaggtt gaaaaaagga ttgaaatgtc
     1921 acatacagca ctgagtttta agaaatattt tcaacatctt aaacagtcgc tggctgtgct
     1981 cctgaggtta aaagataccg tcatactact gtttctgacg accagtatga ttgccatctt
     2041 ggatgtgtcc cctcggctga ttgccctccg cttcatccaa cagacactag cacaactgag
     2101 cattgggcaa ctcttcgccc tgctctccat catcatgtct tgtggagcta tccttggcaa
     2161 tatgaccagc agtaatctat ttaaaaatat ccgtttcacg cacctcttgg ttttctgtga
     2221 gatttcccta ttgactctaa taactagtat cctttgtcaa gcctatatcg taattttcat
     2281 gaccagtttc atcagttcta cgattatcgg cattctcagc cctcgcctac aagcagctgt
     2341 ctttgcccat atccccagtg acaagatggg gacggtgggc tctgctctga gcacagtgga
     2401 cattctcgcc ccgtccctgc tctccctatt agccctatcc atagcatcgg gcgtttcggt
     2461 gcagttagca tcgatatttt tgtatcttat tttaattgct cttatctttt gtcaatggtt
     2521 agtcaagttc aacactcata actaacgaaa aagcatgtgt agatttcaca tgcttttaat
     2581 ctccccaatc gtcaggtcaa gtacaacaaa gtcacttctt tgattaagcg agtgttctaa
     2641 tataattata agcgccctgt cattaccgaa cccattcgcc attatagttg acagaatagc
     2701 catctacggt cgtattcact gccaaagcac ctgagctata agcatagtac cagttgccat
     2761 tgacctggaa ccaacctgtc ttcatgtctc cattacctgc atttaggtag taccaagttg
     2821 aaccatcttg ataccaacca gttgccatag ctcctgatga acggagatag taccatttgt
     2881 tcccaaggtt ttgccagcct gttttcatat cgccatttgg ctggtctaaa taataccaag
     2941 tggtaccttc ctgataccag ccagtggcca ttgctcctga ggaacggagg tagtaccact
     3001 tattacctag atattgccaa cctgtttgca taataccagt tgttggatct aaatagtacc
     3061 aagtcgaatc atcgtttatc caccccgcac gtctttcacc accaaggtag ttttctccat
     3121 taatttccgt cttagctaga taataccagt tagactgatc ataaagccaa cctgtctcta
     3181 aagaatgatt ttgattaaag taatagttcg tataataacg tttctcttct ttatcttctg
     3241 aatcttcacg tttttccccg tactttcttc caacactgtc tttagtttta atctctaatg
     3301 ttttccaacc aacaaactct tgtagcactc catttttatc gaagtagtac cactctgact
     3361 ttggaaaacc ttctaatctg ataccatttg ggtaaggacc aattgtacta cctttagatg
     3421 gaaacgggat atattgccag ccgacaacca tctctccaga tagagaatca aaataatagt
     3481 acttaccatc aatcactcgc cagtaggttt cttttagatc cccctttttg taataggttc
     3541 taccgttttc ttggacaaac tgccatcctt cagagttatc tgcaaatact gtattcgtag
     3601 ctagcaaacc aaagaaaatt actgttagtc cagcttgcat agtttttttc aaaagcttca
     3661 ttttatatat cctccaacat taaatccact caccagatga agcgaagtta taaactttac
     3721 catcgatagt ttgactacct gtaaccattg ctccatttga attgagataa taccattttc
     3781 catttacttt tgtccaacct attttcatat ctccattaga aggatctaaa tagtaccaag
     3841 ttgaaccatc ttgataccaa ccagtcttca tatcaccatt tttattatct aagtaatacc
     3901 atttgcctcg tacatatttc caacctgttg ccatagaacc agaggagcca aaataatacc
     3961 attttccgtt tactttttgc caatctgtaa gcatcttacc tgaagcatct aggtagtacc
     4021 atggagcagg tttgcttcta tcaatgttaa tagtaacatg aaaatcttga gtccaaccct
     4081 tagcaacttc accaattggt agtggattgt aagaatcatc gccaaaattt cctagcttat
     4141 ttaaataata ccaatgaccc tcttcataaa tccagcctgt ctttacagca taattattat
     4201 caaaataata agttctcttt tcagcagata gaggatattg ttcaccgtat acttttcctg
     4261 tattttcaga cgtttttgcc tctagtactt gtttatctgt ttgttctagc aaagcaccat
     4321 cttgtccaaa atagtaccac ttctcctgaa ggccaatttc attaactggt tggttatcga
     4381 ataaattatc acgataacct gttccaggaa tttctaagta ttgccaaccg acaaccatct
     4441 ctcctccatc accaaaatag taggttttac cgtctatttt atgccaatag attgctttat
     4501 gatcatcttt tacataatat tttctattat ccttatcaat aaatcggcca cctgtggtat
     4561 tcgcaaatac cgtatttgta gctagcaaac caaagaaaaa gactgctagt agaacttgca
     4621 tcattttttt caaaattttc atctatatac cctcctaaaa cgattgtagc aaagacccga
     4681 ctgcatgtca atatataaaa actcctcaaa aaagcagtta cacaactgca actgcttttc
     4741 tattattgca tggtgtaacc aacaccacgc acggttttaa tgtagctttt ttggccttta
     4801 acatcaagct tgctccgtag atagcggata tagacatcta cgatatttgt ctcggtcgca
     4861 ctttcatact tccaaacact ttccaacaat tgctcacgag tcaatacttt cttgcttccc
     4921 ataagtgtcg ccaaaaggtc atactcacgg cgggtcagag caatcatctc ttcaccacga
     4981 taaaccgtgt gatgttcaac atctatccta agattgcggt aagacgttgg aaccttcatc
     5041 tgactacagt gttgatcgat aaagtcccga cctcggaaaa tcgctgaaat acgcgctacc
     5101 agattctcaa taataactgg cttatagatg taagaaacgg caaagcgttg gattgtctca
     5161 atctgatctt gcaattcttc acgatgatcc acgaccatga tgactgaggc tggcttagtt
     5221 cgactcagct tgtctgcaaa atcctgagct gtcatatccc ccagatgagc attcaataaa
     5281 atcaagtcat agtctgtctg aagagccatg gagagagctt tttgcccctc ctcaacccga
     5341 tcaactcggt attgctcttt ttggagttcc aaacttaaaa agtaagctag atttcgttct
     5401 ttctcaagta ataaaatccg tttccccatg gctgacctac ttatttttcg tcataccaag
     5461 agtagtggaa ggttccttct ttgtctttac gttggtaagt gtgggcacca aagtagtcac
     5521 gttgtgcttg gatcaagtta gctggaaggt cagctgaacg gtagctatca aagtaagtaa
     5581 tagccgctga gaaagttggc actggcacac cagcttgaac cgcaagagct acgatatcac
     5641 gcactgcttg ttgatactta gcagtaacat ccaagaagta ttcatccaaa agaaggttgg
     5701 caaggtctgc atcacggttg taagcatctg taatcttttg caagaaacga gaacggatga
     5761 tacagccatc acgccagata gatgcgatat ctgcaaatgg caagttccag ttgttttctt
     5821 tagaggctac acgcaattga gcaaatcctt gtgcgtatga aatgattttt gagaagtaaa
     5881 gggcttgacg gattttttca atcaattcag ccttgtctcc ttcaaacttg aaggcagctg
     5941 gttttggaag caccttgcta gcatgtacac gttcttcttt gtaagttgaa atgtagcgtg
     6001 caaacactga ctcagtaatc agtgacaatg gtacaccaag gtcaagagat gattggctag
     6061 tccatttacc agttcccttg ttacctgcag catcaaggat gtagtctacg attggtccat
     6121 cttggccttc atcgtcttta cggctcaaga tatcagctgt gatttcgatc aagtagctgt
     6181 ccaactcacc cttgttccac tcagtaaaga tgtcagccat atcttctgca gaaaggccca
     6241 gcaagtgttg catcaagtca tagctttctg cgatcaattg catatcaccg tactcgatac
     6301 cgttgtgaac cattttcaca tagtgaccag ctccatcagg accgatataa gtcacacatg
     6361 gtttgccatc ttctggtgct ttagctgaga tttcttcaag aacatcagca accaattcgt
     6421 aggcctcttt ttgtccacca ggcatgatag aaggaccttc aagggcacct ttttcaccac
     6481 cagaaactcc agtaccgata aagttgatac ctgagtttgc caattcttca ttacgacgga
     6541 tggtatcttt gtagaaagta tttccaccgt caatcaagat atcacccttg tcaaggtgcg
     6601 gaagaagggc ttggatagta gcatctgtac caggtccagc ttgaaccatc agcatgatac
     6661 gacgaggttt ttcgattgag tttacaaaac tttcaacgtc atagcttggt acaaaattct
     6721 tttcaggatg gcaagcaatc acatcttccg ttttttcttt actacggttg tagatagcaa
     6781 ctgtgtaacc acgtgattca atattaaggg caaggttacg acccattaca gccataccta
     6841 cgacaccaaa gttagctttt gtcatttgac actcctcttg tttcatttgt tttattttat
     6901 catttttact tttgaaaaga aagaattttg taacgactgc ttagtagtcc aagtcatccg
     6961 catgaccagc gccatttccg acaaagtagc ctgtcttaca gttaagggta aagagatagg
     7021 ttccatctgg cttatagagg ttgtaataac cattgccatt aacgatattg acacgttcaa
     7081 ggatatattg gtctccagtg atatagccac gtgaacgtga agtcgctaga atttgttcta
     7141 agacaccatc cgcaaaatcc caggcagagt tgttactatc atcaatagca gactgattat
     7201 aggcaacacg gctggcactt ctttgaacag caacccctgc actcgagaga cccatattga
     7261 cttcactgcg actacttcta gcctcatttg aactacttgg ttttgtctca ctagtcgtat
     7321 ttgaacttgc ttgacttgag cttgagctag aagtacttgt ttgctggctc ttaccaagac
     7381 tgatagcctt atctagcact ttatctagct ccgtatttcc agttttaata tccgtaaatt
     7441 tggcatccga tttggctttg gcattggtat ccaacacacc atccacaata gctggtttct
     7501 caaattgagc attgacatct tgaatggcct tgatttgcgt tgctagacta tcatacttag
     7561 atttggctag cgtatgttca cgactacctt ccagtttatc aagcaaggtt ttgagttgac
     7621 tcagtttatc aaactggcta ttcttcaaag ctgttttatt tccatctgtg tagaaggtat
     7681 cataaagtgt attaaaatca tccacatctg attgattggc tgtagttgat tgagaagctt
     7741 caatttcctt ggtcgagcga ttcacttgac gatagacata ataagcactg acacagataa
     7801 tcactgatac aagagccaaa gcggtcaaaa caaagccttt tttacttttc ttttctgagt
     7861 cctcttgttc cagacgagaa aggttttgct cctcttccac ttcctcagtc atttctgttg
     7921 gattttcatc ctctagcaat agaggatcca agccttgctc ctcatcgtcc agaggcaagg
     7981 gtagtaagat gtcttcagaa gatggaactt ccttcgaata agcttctgac tcctctacag
     8041 cctcacgcat ctcttgaatt aaatcatcca gactctgagt ttcaactaac tcctcttttt
     8101 tgtattgacg agtcgcaaac ttatccgcct caatttcatc tctgtgttgc ttaacatact
     8161 tgtccaaaat ggaatcctca ggcaagactc ctacttccac ttcttcattt ttacgaatag
     8221 cttgaccaac tgttagctct tttgcttcat caaactcaaa ttgcggttct tgaccttctt
     8281 ttttatgacg atttcgtctt tttttactca tgagtatccc tttctatttt acctcttgcc
     8341 gtttgacacg ctgaccaaaa tattgataca gatccacttt caaggttccg ttgtataact
     8401 tacgcttctt atctgcttgg ctaccatact tgctttcaaa agcttcatca ctagtcagga
     8461 taaatttgct ccaagttttc agcggtgcaa atacttgccc catctcagca tagagcttgg
     8521 tcacccctgc atcatctgac aaacgttcac cataaggcgg attggaaatg attactccat
     8581 tgattttatc ggaacgtaaa tcctgcacgc gcatctgctt aaaagtaatg tctcctgcaa
     8641 caccagctgc ctgagcattg gacttagcaa tttccaccat gcgagcatca atatcacagc
     8701 ccatgatatc cagctcaagc tcacggtcta cttttttagc cgcttctgtg cgcacttctt
     8761 gaatcaagcg atcgctgatc cagttccatt cctcaaatgc aaaagagcga cgaagacctg
     8821 gcgccatctt tctagcaatc ataactgcct caatacagaa agtccccgaa ccacaggtcg
     8881 gatcaatcaa agacttgtct ggataccagt tagaaagttg taaaatggct gctgccatat
     8941 tttccttgat aggagcgcca cctttttcgg tacgataacc acgtttaaag aggctagacc
     9001 cggtcgtatc aatcatgaca gttgccacat ctttgagaat agagacctca atcttaaact
     9061 ctgggccatt ctccatcaga ggaacccctt ttgggcgagc atagtgtttc tgcaatttct
     9121 tgacaacagc tttcttagaa atagcctgaa cactgggctc attgtgaagt ttggacttaa
     9181 cacatttagc ttttgaaatc gggaaccgag ctccaagtgg taaataattt tcccaatcta
     9241 aagcgaaaac tccctgaaat agctcttcaa aagtcttagc tgggaacgtt cctacgataa
     9301 ttttgatacg atctgctgcc cgaagccaaa ggttggtttc gataatagct ctcacatctc
     9361 cttgaaaacg aacacgtcca ttttcaacct gacaatcgta gcccaactct cgcacttcac
     9421 gaccaacgac agcctcaagc cctgctgcca cagttgcaat taagttaaat tcttttttca
     9481 tgttacagtt ttgcaagacc tttctatatg tccactttaa aaaatgaggt tgagaaaatt
     9541 tctcagcccc aacctatatg caattttcta taagccatgt tttgttccag aagtgactag
     9601 gaccacttcc ttcgataatc atctgtctac cactaacagc tctagctgat agcagtcaga
     9661 atactacgtt cgtttccgcg tactccatgc cccgaccaaa atttgggttg ctagcttgag
     9721 gggtttaccg cgttccactc tctctgtttc cagaaagact ccgtcactgt ggcactttca
     9781 agcctactct ggcctatcca aggacttagc catttcaact gccgtaacga tttctcgtcc
     9841 ctaggcttat ggtttcgcct agcacaaaca ctacaggcat cacagcctgt gctagcatgg
     9901 actttcctca tgagaagcaa ttctcctcac gcgattatcc aaaaattgca catctcaaat
     9961 aactacttaa aaatctgagt tatctaaaat ttgtttacca aaaacttctt tttccaatct
    10021 attcaggcgt ttcaaaatat caaaattcgt catagaactt gtaattgccg cttcaagggg
    10081 ttctgcttga actggtgaag gtttcggttt acgagttaat tcttccttca aatccgcaat
    10141 ttcctgacga agtgacttga ccaaggcagc ataggtttca tagtccttga tgacatcgtc
    10201 taaaaactcg tcaacttcta ctttattata gccacggact tcacgtccaa actcttgttc
    10261 aaaaatatct ttcgctgaaa aaataatact tgccatgtct ctctccattc tcgcttgcta
    10321 gtattattat ataaaaaaag gcaaacaaaa atcaaggtca aagcttcaat tttcggaaaa
    10381 attttcagca agttcattta attgatcaaa tgttaatctc tttataaaat agtcctcttg
    10441 atttttcatc ttttggtaaa aataagctag ttttgtttca ttttcttcat cataaaaaag
    10501 ataggaacta gtcgtgtttt ccagcaaaaa ctgccggtaa tctcttaact gccccttgtg
    10561 ttcatagcga ggataggcat atttgacaaa gtctacctgc ttaaaacgac tcagtttcat
    10621 ctgattgcct tcattccaat tttccccatg ggtttcaaaa gcaaaaatag tcgccaactg
    10681 gaaaccgtac tcagttttca tctcctgagc aacctctaaa acccaatatt caaagcccaa
    10741 acttcctgta aatacaagcc aagacacccc atctgctgcc atagattcta aatctttacg
    10801 tattgctttt ttaatcattt taagacgagg atccttgtca gaaaacaaac ccaaatcaaa
    10861 agcagaatag cctaaaacca aagctgtagc catttttaac cctttctgtg caaatttatg
    10921 gtataataaa gtgatgttat tgtaccaata aggagaatta tggtcaacta tccacataaa
    10981 ctttcatcac aaaaaagaca aacatctctt tctcaaccca aaaatttcgc aaatcgagga
    11041 atgtcttttg aaaagatgat caatgctacc aacgactact atttgtctca gggcttagct
    11101 gttatacaca agaaacctac tcctattcaa attgtacgag tggactatcc acaacgaagt
    11161 cgtgccaaga ttgttgaagc ttattttcga caggcttcaa cgacggacta ttctggcgtt
    11221 tacaatggat attacatcga ctttgaagcc aaggaaacaa aacaaaaagg tgcgattccg
    11281 atgaaaaatt tccatccaca tcagattcag catatggaac aagtccttgc ccaacaagga
    11341 atctgcttta tccttcttca cttttcttct cagcaagaaa cttacttatt gccggcattt
    11401 gatttgattc gcttctatca tcaagataag ggacaaaaat caatgccact tggatatatt
    11461 cgagagtatg gatatgaaat caaggctggt gccttccctc aaattcccta tctcaatgtt
    11521 atcaaagaac atttattagg tggtaaaaca agatgaaaaa accaacgatt ctgcgcctac
    11581 tcaagtatct tagcattagc ttcttaagct tggttatcgc agccattgtc ttaggcggag
    11641 gagttttttt ctactacgtt agcaaggctc ctagcctatc cgagagtaaa ctagttgcaa
    11701 caacctctag taaaatctac gacaataaaa atcaactcat tgctgacttg ggttctgaac
    11761 gccgcgtcaa tgcccaagct aatgatattc ccacaaattt ggtcaaggcg atcgtttcta
    11821 tcgaagacca tcgtttcttc gaccacaggg gaattgatac catccgtatc ctaggcgctt
    11881 tcttgcgcaa tctgcaaagt aattccctcc aaggtggatc aactctcacc caacagttga
    11941 ttaagttgac ttacttttca acctcgactt ccgaccagac tatttctcgt aaggctcagg
    12001 aagcttggtt agcgattcag ttagaacaaa aagcaaccaa acaggaaatc ttgacctact
    12061 atataaataa ggtctacatg tctaatggca actatggaat gcagacagca gctcaaaact
    12121 actatggtaa agacctcaat aatttaagtt tacctcagtt agccttgctg gctggaatgc
    12181 ctcaggcacc aaaccaatat gacccctatt cacatccaga agcagcccaa gaccgccgaa
    12241 acttggtctt atctgaaatg aaaaatcaag gttacatctc tgctgaacag tatgagaaag
    12301 cagtcaatac accaattact gatggactac aaagtctcaa atcagcaagt aattaccctg
    12361 cttacatgga taattacctc aaggaggtca tcaatcaagt agaacaagaa actggctata
    12421 accttctaac tactgggatg gatgtttaca caaatgtaga ccaagaagct caaaaacatc
    12481 tgtgggatat ctacaactcc gatcaatacg tctcttaccc tgacgatgat ttgcaagtcg
    12541 catctacggt cgtagatgtt tcaaatggta aagtcatcgc ccaacttgga gctcgtcacc
    12601 aagcaagtaa cgtttcattt ggtaccaacc aagctgtgga aaccaatcgt gactggggtt
    12661 ctgctatgaa accaatcacc gattatgcac ctgccataga atacggtgtt tatgattcca
    12721 ctgcaactat ggttaatgat attccttata actatccggg aacaagcaca cctgtctaca
    12781 actgggatag agcatatttc ggtaatatta ctctgcaata tgctcttcaa caatcacgaa
    12841 atgtcacagc cgttgagact ttgaataagg tcggtctaga tagagctaaa accttcctta
    12901 atggtcttgg tatcgactat ccaagcatgc attatgcaaa cgccatttca agtaatacaa
    12961 cagaatctaa taaacaatac ggagcaagta gtgaaaaaat ggctgctgct tatgctgcct
    13021 ttgccaacgg tggaatttac cacaaaccca tgtatatcaa taaggtcgtc ttcagtgacg
    13081 gtagtgaaaa agaattttca gatgtaggta cacgagctat gaaagaaaca actgcttaca
    13141 tgatgaccga aatgatgaaa actgtcttgg catacggaac tggtcgtgga gcctatctcc
    13201 catggttagc gcaagctggt aagacaggta cttctaacta cacagatgat gaaattgaaa
    13261 aacacatcaa gaacactggc tatgtagctc cagatgaaat gtttgttggt tatactcgta
    13321 agtattctat ggctgtatgg acaggttatt cgaatcgttt aactcctatc gttggagatg
    13381 gtttcctagt tgcagctaaa gtttatcgct caatgataac gtatctatca gaagatactc
    13441 atccagaaga ctggacgatg ccagacggac ttttcagaaa cggggaattt gtattcaaaa
    13501 atggagctcg cccaatatgg actgaaccct ctactcaaca atcctcaaca gctgaaagtt
    13561 caactacaca ggcaagtact acggttggtc aacaacccaa taatgctcca gcaactgatc
    13621 ctaatcaagg acaagctgtt caaccgacac aaccagtaca accaacgcca caaaatccac
    13681 aagttcaaca gcaaccacaa cagtaaacga aaaaatcctg agaactctaa actcaggatt
    13741 ttgtcatttt caaaatgaaa tacagagatc aagtacattt ccccacaata aaacgcatac
    13801 gatcaaggct ttcatcacct gacaatatgc gttttgcttt taaagacttt ttgctcagtc
    13861 tctttttatc ttatttcaca tgttttgcga gttcttcttg cgctttttta ttggattctg
    13921 ctttttcttt catccatttt tcttgagctt tttgatattc atctgcagta actgccttgt
    13981 cttgaagttc aaggtattta tacaagactg ggtcacttgt acctttattt cctgacaatg
    14041 caaatggtat tgtaaatggt accatcttag acaagattgg acgaccagtt ttagaagttg
    14101 ttgggatgat caaggcacta tctgtcaacc aagcttgggc tgctgcgtat ttatcatatc
    14161 gtttagcaac atctgtagtc tcatcaccag cttcagtaac caatttttcg tagtcatata
    14221 gacctacttt tttagcagct acattatctt cccctgagtc aaatcctaaa tatgttttag
    14281 tactttctcc tacagaaggt ttgataatat caaggtaggt tgatggatcg gcaaagtctg
    14341 gaccccaacc gacattatct gataaatccc agtcttcgcc agcagcattt tcggcaaata
    14401 gagttacatt aagcaattca tctttttgca attgttggat atcaataacg acattatcag
    14461 ttcctaaagt tgcttccaag gattgtttca tagattggac acgttgaact tttgtagttg
    14521 ctgtctggtc aacaggcata tctaggtgaa ttgggaattg tactccctca gcttgtaggg
    14581 ctgatttggc tttggcaaat tctgctttag ctttttctgg attgtaaaga ccatcttgtg
    14641 aatctgcaag attgacatcc ttccattcat ccccataagt cactaatttt tctttgacta
    14701 tatcgccaaa gttcttccca tctgcttgaa caaatgttgg tggcacaaag agattacgca
    14761 agattttgct tgctccagtt tctccattca actgagaagc ataagctgta cggtcaaaac
    14821 caaaggcaat agcctgacgg aaatccttgt ttaagagagc ttttttagta gatgtctttt
    14881 gttcgtcgct agtcttagat gtgtgtttat aagattgacg gtcaatattt gttcctacta
    14941 aataagtcgt agagtcttgt tgagtataga cgatattgtc tttcatactc ttctcaagct
    15001 ctgcgaaact tgcacttgtt ggataaagac gagctgctgt aaggctacca tctttaaagt
    15061 tttctgctgg tttgctggta tcttgcccat cccagaatga caatttgacc ttgtcaatat
    15121 gcacattgtc tttatcccag tagttcggat ttttcgcaaa ttcaacagag gatttagtta
    15181 caatggattt caacaaataa gggccgttat acaagagact acttggatcc gtagctttgg
    15241 caaaatcatc cccttttgaa ttcaaaaact cttcattgac tggcgcaagc acacccatgg
    15301 ttgttttaga attccaaaaa gtttcaggtt tgttcaaggt gtattggact gtctgatcgt
    15361 caagggcttt aatcccgact tgagcaaagt ctgtgatttc acctttggca taggcatcca
    15421 gacctttgat tgaatcctgt accaagtaaa gggcttctgc tttcttatct gttgcatatt
    15481 taagtcctgt tacaaagtcc tgagccttaa caggagcgta ctcttctccc tcagatgtat
    15541 accacttggc atcctgacga atcttataag tataagtcaa gccatcctta gagaccgacc
    15601 agtcttctgc cattgaggga ataaggttcc cgtatttatc attttcaagt aatccatcga
    15661 tcacattact agtaatctca gaagttgctg ccttaccagt tgtcaaatag ttgagattat
    15721 ctggatgtgt ctcgtaggta aaggcaaagg ttttttcacc ttgagcacct gaacccccag
    15781 aacaagcagc taaaacacca gctgctaata aggttactcc cgcaagagcc aatacttttc
    15841 ttgttttcat agtcttctcc tttgtttttt tattcattat agactctttt tcaagtcctg
    15901 tcaatagaat tttctgaatt ttaagagtta tttgttatat ttgatatatt ctgaaaaata
    15961 aaattgttca tttatgccaa aaaaaagcca acagaagttc tcttctgata agcagaaaag
    16021 aatcttgttg accttacttc taaaaatctt aagtagcaaa gattctaaaa tgacagaatc
    16081 cttgcattat tgtcccaact cttccgcgac tacttccaaa gcccgttcaa tgaccacatg
    16141 catgtcatag tatttataat ctgctaaacg accacagaaa ataaccttgt catttgttgc
    16201 cgcttcttcc tgatatttgg caaaaatagc attgtttctc tcatcattga ttggataata
    16261 aggttcatct cctcgtttcc aatcagctgg gtattcacga gtaatgaccg ttttagcttg
    16321 tgtaccatac tcaaagtgtt tatgctcaat aatgcgagta taaggaattt ctcgttctgt
    16381 atagttgaca actgcgtttc cttgatagtt ttcttcattg agaacttcat gctcaaaacg
    16441 aagactacga tattctaact caccatgttt ataatcgaag tattggtcaa tcatccctgt
    16501 aaagacaacc ttttcagcag aagcttctaa ttcctgacga ttggcaaaaa agtcaagacc
    16561 aagttctact tccacatcct tgagcatatt ttcgatgatg acgttgtaac cgccaattgg
    16621 aataccctgg taacggtcat tgaagtagtt attatcaaaa gtcaaacgaa ctggtagacg
    16681 tttgatgata aagggtggaa ggtcagtcgc agaacgtccc cattgctttt cagtatatcc
    16741 tttaatcaat ttttcataaa tatctggacc gatcaacttg atagcctgtt cttccaaatt
    16801 tttaggttca acgtccttca tgtgagccgt ttgctcagca atcttatctt tcacttcttg
    16861 aggagttttt gtctcccaca tagcatagaa agtattcata ttgaaaggta gattataaag
    16921 gctacccttg taattagcta caggcgagtt gatgtagttg ttaaattcag caaattgatt
    16981 aacataatcc caaacttttt tattagaagt atgaaagata tgggcaccat atttatgaac
    17041 attaacccct tctacattct cacagtagat atttccacca atgtggtcac gtttatcaat
    17101 aacttttact ttttttccac gcttggttgc ttcataagca aaaattgctc ccgacaaacc
    17161 agcaccaacg attagatagt cgtacatagt ctcttcctta tcatttacct ctcatctcct
    17221 taactatagc aaaagagagg atctttatac tcttcgaaaa tctcttcaaa gcacgtcagc
    17281 gtccatctgc aacctcaaaa cagtattttg aactgacttc gtcagttcta tctaccacct
    17341 caaagctgta ctttgagcta acttcccagt tactctttga tttttattga gtattcgttt
    17401 caatctattt tagcacaaaa caaatcattt ctcagttttc aatagccgag tgatttaaaa
    17461 agacaagcac caaaactgat gtttgtcttt aggattttgc ttgaaaaggc tatttctaac
    17521 tatttaacat gtttttctgc ttctttttgg gctttttcga ttgctttttt gctttcttgc
    17581 tcccatttgg tcttagcttc ttcaaactgt ttagttgtaa cagtgtcttt ttgcagtttc
    17641 atgtacttgt agttattgcc atcacccttg ataccaacca acgaatagcc tcttgtaaat
    17701 ggcgttactt tggttacaga tgctgttcca ccacttgaca tagctgacat aatcagagaa
    17761 ttgtcaatca tccaagcctg tgcttcagca tatttttcgt aacgtttagc tacatctttg
    17821 ttttcactat ctgcatcttt gagcatctta gtgtaggtat ccagacctag actagtgatt
    17881 ttttccttgt cttccttggc atctagtcca aaaatcttaa ggtagaagcc atcctctgca
    17941 ttgaaaggat tgagataagt tgatggatct tggtagtcac ctacccagcc atcaaagttc
    18001 aaatcatagt cacgatcagc tgctgttggc gctaagaagg ctacattatt aaaatcatct
    18061 gttgaaagtt gctgaacatc aatgacaacg ttatcagcac ctaaaactga ctcaagggtc
    18121 tgcttaactg agttcatacc tgtcacggca tttttacttg tctgatcaac cgccacatcc
    18181 aagtgaatag ggaaagtcac accttgactt gccaattctt ttttagcttc cacaaatttt
    18241 gcttgggctt tttctttgtt gaaataggca tcctgagcat ctgccaagtt aatacctgac
    18301 cattctgtgc catagttgac caatttagaa gcgactactt ctccaaaggt cttgtctcca
    18361 acttggacaa atgtaggagg caccaaggtg ttccgaaggg tcttgctagc cgcctcttct
    18421 ccatttgact gggcagaata ggctgtgcgg tccaaggcaa agttcactgc ttggcggaag
    18481 ttcttgttca agacagctgt ctcagttgac ttcttctgct catctgtcgt tttagatgta
    18541 tggttgtatg ccttacggtt gacgttaaaa ttgaagtacc aagaagtctt gtcctgcaag
    18601 ctatagacga tattatcctt atatttttcc ttagtcttag caaagtttga actatttgga
    18661 taaaccccag cgattgaata agctccactt tcaaagttac ggatggtcaa ttcttggtct
    18721 gagccatcaa aataagccaa tttcacatgt tcaattgata ctttgtcatg atcatagtaa
    18781 tgcggatttt tcacatactc gattgatgat tttgatgtga aatcttttaa caaataaggt
    18841 ccgctgtaga gaatgctatc tggagatagg gtcccaaaat ctttcccttt tgagtttaaa
    18901 aactcttcgt ttactgggaa aagaatacta ttggttgttt ttgaattcca gtaaggttct
    18961 gggcgtgtca aagtatactc gacagtctgg tcgtcaatgg ccttcacccc aaccttagaa
    19021 aagtcagaat ccgctcctgt aatataatca ttcaagccct tgatcgagtt ttgaatcaag
    19081 tcaatggcct gtgatttatt atccactgca tacttgatac ctgtcacaaa atcctgagcc
    19141 ttgactgggg cgtactcttc accgtcagct gtgaaccatt tggcatcttt tctcaatttg
    19201 taggtataag tcagaccgtc gcttgaaaca gaccagtctt ctgccaaaga tggaactagg
    19261 tttccgtggt tgtcattttc aagcaaacca tcaactaggt tggtaatgat ggctgtatta
    19321 tcagcgtagt agtctaatag atagttaaat gtagttggat ttccactaaa ggttgatgag
    19381 taagtttttg tatctgaacc tgattgtccg caagcagcta aaagcaaagc agctgcaaaa
    19441 gtcagtcctg cactaaggat acgctttttt gtagtcatct tctttctcct ttatgttagt
    19501 ttttataaca tatatttatt ttaccaaaat agtgggaatt tgtaaagtta attgaatttt
    19561 tataaccaaa gtttataaac tttggttata aaaaaacaaa ggatttctgc ttttcataca
    19621 atcctccctt gtttttatac gatttatcat ttgaatatca atgaattaac tgataaccta
    19681 acgaaagcaa gattctcatt ctcattattt tatgtgcttt tctaaatcct tttgatactg
    19741 agcattcgat tccagttttt ccttttgcca ctttttgaag gccgcatcat actctttagt
    19801 tgtcacaata tcattttgaa gttccattcc tttaaaaata aatggatccc ccttaatacc
    19861 gacctgtgaa taagctttgg taaatggaac agtacgacta accataggag agccccctga
    19921 agaagcaact ggaataagaa gtgaactatc agtaacccaa gcctgtgctt tggcatactt
    19981 ttcgtatcgt ttatcaagat tacttgtctc agaagccgca tcatccaaga gcttcttgta
    20041 ttcatccaag ccaactttcg ccacaacttc aggatccttg ccctttgtta tacctagatg
    20101 tttcaaggca gaacccttct tagcatctaa aatattgaga taggttgctg gatcttggta
    20161 atctggtccc caacctgttc cgttcaaatc ataatctttt tgggttggaa ctttagcttg
    20221 agaagtaata ctttccttct cattatctgt catttgaaga acatctataa tgacattctc
    20281 cgttccaagc gtttcttcaa ttgattgttt tagcgaattg gtttgttgaa ccgcaactac
    20341 atcagtttgc tcaacaggca catccagatg aatcgggaaa ctcactccct tagcttgcaa
    20401 ttctgacttc gctttttcaa atgcagactt ggcctttgtt ggattgtaaa ttgtatcctt
    20461 accatctgtg agagtcacat ctttccactg ttctccatat ttcagtagtt catcctgagc
    20521 caactgtccg aaggtcttcc cagctacttg aacgtaatta tctggcacta gactattacg
    20581 aataatctta tccgcaccct cttcaccgtt taattgagct gtataggcgt ggcgattaaa
    20641 tgcaaaatta agagcctgac ggaagttctt attgagaaga gcttcttttg ttgatgtttt
    20701 ttgtgactca tctgttttag ctgttttatt gtaagactgg cggtttacat tcacagtgag
    20761 gtagtagctt gttgcctctt gtggactata gacaatctta tctccgtact ctttcttagt
    20821 tgactcaaag tttgagcttg ctggaaaaag acgtgcagtg gtataggcac cttgtgtaaa
    20881 gctacgaatt aaggattctt gatctgaacc atcatagaat gtcaatttaa cattatcaat
    20941 tttgacattg tccttatccc aatagttagg atttttttca tattcaatca cagatttaga
    21001 aatcaaagat ttcaaaaaat atgggccatt gtaaagaata cttgatggag ttggcgctcc
    21061 gtaatccttg cctgttgcat tcaaaaattc ttcatttaca ggaagcatgg ttgctgtggt
    21121 tactttagag ttccaaaaac tttccgggtt attaagggta tattctaccg tataatcatc
    21181 aagagccttc acacctacag ttgagaagtc gtttgtctca ccactaacat aagcctccaa
    21241 tcctttgata gatttctcaa ccaaagaaag accatcagac tttccatctg ctgcatgttt
    21301 cagacctgta acaaaatcct gagctttaac ttccgcatac tcttctcctt ctgaagtata
    21361 ccacttcaca cctttacgta gtttgtaggt ataagtcaaa ccatctttag aaactgacca
    21421 atcctctgct agagaaggaa tgagattccc atatttatca ttctctaaaa gaccgtcgac
    21481 gacattacct attacgtcag aagttgaact tttacttgta aggctataat ccaaggatga
    21541 tggatcaact gcataaacat aagaaaatgt tttgggagca tctgcattct tttcactttt
    21601 cccacaagct gttaaaagaa tggctgtact caaggtcact cctgctccta tgagccactt
    21661 tttcgattgc ataaataatc tccttcaaaa tagtattttc actattatac cctatttttt
    21721 tataaatgca agagttctta tggaatttgt tagaaaattc taacaaatgt ttttaaaaga
    21781 gtttttacac aacttttagc aaaaagaggc tgggaaaaaa gtctttaaaa caaaaaacgc
    21841 ataatatcag gtcttgaaaa aacttggtac tatgcatttt attgtggaaa gatttacttc
    21901 attttctcct gaagtcgagt ttttgcccac tctctctcat tttagtcagt caattccaca
    21961 cagaaagcat cccatggagc taagatttgt ttttcaaaga cttcttgagc cgtggtgttt
    22021 tcaatcaaga cagatttgac tgttccttct actgttaagt cttgctcttc attggacaag
    22081 ttggccacaa ctagaaaacg acggtcgcca tctttacgga tataagcaaa gaccttgtca
    22141 gccgtatcaa gcaattcaaa gtcagctcga atcagccagc tattctcctt gcggatttga
    22201 acgagttttt tataagtata gaaaatagaa tctggatttg ccagtgcttc ttgaacattg
    22261 atttcttcgt agtttggatt gactgccaac caaggctgac ctgtcgagaa accggcgttt
    22321 ttgctctcgt cccattgcat aggggtacgg gcattgtcac gaccaatgac acggatactg
    22381 tccatgattt cttccatcga aacacctttt tcaagagctt cacgcgcata gttaagagat
    22441 tcaatatctt ctacttggtc cagtgtttca aagggatagt tggtcatccc aatctcctca
    22501 ccttggtaga tataaggagt ccctctcatg agatgaagca agattgcaaa ggctttggca
    22561 gatttttcgc ggtattcttg gtcatttccc cagattgaga cgatgcgagg aaggtcatgg
    22621 ttgttccaga agagggaatt ccaaccgtcc tcaactccta actctgtctg ccatttgttg
    22681 aagatttctt ttaacttagc gatattcagc tctttttgat agtgccattt aggttgccct
    22741 tcctgatact gaagaccgat gtgttcgaac tggaagacca tagacaattc ttgccccttt
    22801 ggatctgagt agagcttggc aatcgctggc gttgctcccc aagtctcccc tactgtcaag
    22861 agattcttat ctccaaaggt cgcctgattc atctccttga gatagggatg gagcatagga
    22921 ccattattga ctactttctc gtcaggaatt ttgccaatca tgtcaatgac atccatgcgg
    22981 aaaccaccaa tgcctttatc aatccagaag ttcatcatct cataaatttt ctgacgaagt
    23041 ttttcatttt cccagttgag atcgggctgt ttcttactga aaaagtggag ataatattga
    23101 cctgactttt catcgtattc ccaagcagaa ccactaaaga tagaatccaa atcattgggt
    23161 tcatcccgcc aaatatagta gtctctctca ggactatcag gattttcaca ggcctcgaca
    23221 aaccaagcat gttcatctga ggtatgattg accaccaagt ccatgatgat acgaatatca
    23281 cgcttcttgg cttctgcgat cagttggtcc atatcctcca tggttccgaa aatagctgca
    23341 attgcttgat aatcagcaat atcgtagcca ttatcatcca tagggctgtc ataaacggga
    23401 gaaagccaaa tcgctgtgat tcctaactta gctagatagt ctaacttact ggtaatacct
    23461 ggcaaatcac caattccatc tccgttgcta tccataaagc tcttaggata aacttgatag
    23521 actacggcat tgtgccacca tttttcttgc atcttcttac ctcattattt taataatcta
    23581 tagtctatga tagcgctttt tagaattttg tgcaagcgtt tgcctaatct ttttaatttc
    23641 ttttttgact ccatagtttc ctaacttcca attttttatt ataatagagg tcagaggtaa
    23701 aaaaatgaaa aaacaagctt ttagttctga acaatatttg aatctacaac gcgaccacat
    23761 cttggagcgc attaatcaat ttgacggcaa gctctacttg gagtttggtg gtaaaatgtt
    23821 agaagatttc catgctgctc gtgtccttcc tggttatgag cctgacaaca aaatcaagct
    23881 cttgcaagaa ttgaaagagc aggttgaggt tgtgattgct attaatgcaa gtaatatcga
    23941 gcattccaaa gcacgtggtg acttgggcat ttcttatgac caagaagttc ttcgtttgat
    24001 tgacaaattt aatgaattag ggatttttgt tggttccgtt gtcatcacac agtacgctgg
    24061 ccaaccagct gcagatgcct tccgaaatca acttgaaaaa aacggaattg attcttatct
    24121 tcattatcca atcaaaggat atccgacaga tatggatcac atcatttcac cagaaggtat
    24181 ggggaaaaat gactacatta aaaccagtcg caacttgatt gtcgtaaccg ctcctggacc
    24241 tggttctgga aaattggcaa cctgtatgtc caatatgtac cacgaccaaa tcaatggcat
    24301 caagtctggc tacgctaaat ttgaaacctt cccagtttgg aatcttcccc ttcatcaccc
    24361 agtcaatttg gcctacgagg ctgccacagc tgaccttgat gatgtcaaca tgattgaccc
    24421 tttccatctt caaacctatg gagaaaccac tgtcaactac aaccgtgata ttgaaatttt
    24481 cccagttctc aaacgcatgt tagaacgtat ccttggtgaa tctccatacg cttcaccaac
    24541 agacatgggt gtcaacatgg ttggtttcgc tattacagat aatgaggctg ctatcgaagc
    24601 ctctaaacaa gaaatcatcc gtcgctacta tcagactgtt cttgatttca aagccgaaaa
    24661 agttagcgaa tctgctgtca agaaaattga gttgcttatg aacgacctcg gtatcacacc
    24721 ttcagaccgc aaggttgctg tcgttgcgcg tcaaaaagca gaagaaactg gcggaccagc
    24781 cctagccctt gaattaccaa gcggagaaat cgtcacaggt aagaactccg aactcttcgg
    24841 ccctacagca gctgctctta tcaacgctat caagaaatca gctgacattg ctaaagaagt
    24901 aaaactaatc gaacctgaac ttgtcaaacc gatccaaggt cttaaaatcg atcatctagg
    24961 tagccgcaat ccacgcctgc attcaaatga aatcctgatt gcacttgcta tcacagctac
    25021 agaaaatcct gatgctgccc gcgctatgga agagcttggt aacctcaaag gaagcgaagc
    25081 ccactcaacc atcatcttaa ccgatgaaga caagaatgtc cttcgcaaac taggtatcaa
    25141 tgtaaccttt gacccttact accaatacga ccgcttgtat cgcaagtaac gatttcaacc
    25201 tctccaccct cggagaggtt ttctctctgt ctcaggagcc ggctttatgt tataatgaaa
    25261 ttagaaaact gaaaggattt accatgtcaa aagaagttat tgttgaaagt tttgaacttg
    25321 accacactat tgttaaagca ccctatgtcc gcttgattgg ggaagaaaca gggccaaaag
    25381 gagacatcat ctccaattat gacattcgct tagtacaacc caatgaagac tcaatcccta
    25441 ccgctggtct gcacactatc gagcacctct tagccaaact catccgtacc cgcatcgacg
    25501 gcatgattga ctgttcacca tttggttgcc gtacaggttt ccacatgatt atgtgggggc
    25561 gtcataccag cgccgagatt gcagctgtta tcaaggattc gctcaaggaa atcgctgaaa
    25621 ctactacttg ggaagacgtc cctggaacaa ccatcgaatc ttgcggtaac tacaaggacc
    25681 acagcctctt ttctgctaaa gaatgggcaa aactcatctt ggaacaaggg atttcagatg
    25741 atgcttttga gcgtcatgtg atttaaacat aaaaaaagga accagttttt ttcagctggc
    25801 tccttctttt tttattctca aaattttgtc ttatactcaa tgaaaatcaa agagcaaact
    25861 aggaaactag ccgcaggctg tacttgagta cggcaaggcg acgttgacgt ggtttgaatt
    25921 tgattttcga agagtattag gctttttcac gaatgactaa aacgccatct tccatatctg
    25981 cttccagatg tttggcatct aggtgatcca aatggaagtc tgtcacttta tcacgaattt
    26041 cttgttcaac cacgcgacgg agcggacgaa cacccatgac ttcatcgtaa ccttcttctg
    26101 tgatatagtc tttagccgct tgactgacta ccaaatcaat ctctttctta gctaaggttt
    26161 ggttgacttc agccaacatc aagtccacaa tcttagaaag gtcttccttc gtcaagtgcg
    26221 agaactcgat aactgcgtta aagcggttga ggaactctgg acggaagaat ggtttcaaac
    26281 ggtccatcaa ttctggttta tccgcatctt ctgtcaagtt ggcttcataa ccaaatccag
    26341 catttgaggt cgcgataatg actgtgttct tgaagttcac cgtatttcct tgaccatctg
    26401 tcaaacgacc atcatccaag acttggagga gaagagtgat cacttgagga tcagcctttt
    26461 caatctcatc caagagaatg atagagtatg gattgcgacg aacacgttct gttaaggtat
    26521 tgctattatc atcataaccc acataaccag ctgttgtacc gattagctta gaaacggctg
    26581 tgcggtcact gtattcagac atatccaaac ggataatcgc atccttggtt ccaaacatat
    26641 caagcgccaa ttgcttagca agctccgtct taccaacccc agttggtcct acaaagagga
    26701 agctaccaat tgggcgatta ccttcatcaa aaccagcacg attacgacgg atagcttttg
    26761 ctactgcttc aacggcctta tcttgaccaa taactttcgt ctgcaaacga tgacccatat
    26821 ctttcaaacg ttcgatgtcc gtagctccca tttgtgacac tggaataccc gtcattcgtt
    26881 ctacagattc agccacatcg ttgacacttg cagtcacttt catatcttct gtgtggtttt
    26941 cgattttctt ttccaattct gcgatgcgtg ttttatagtt gagagctgct tcaaaatctt
    27001 ccgcctcaac tgctttttct tgcttgtctt tttctgcctc aatttcacgt tcaacagcat
    27061 gcacatctgt tacaggatgt tgagccgcca agtgagctgc cgttacatcg acaaggtcaa
    27121 tagccttatc tggcaagcta cgttgaggaa tatactgaac agaataatcc actgccgctt
    27181 tcaaaacttc gtctggcaag atgacattgt ggtgttgttg atagagatca cgaattcctt
    27241 gaaggatttt aaaagtatcc tctgctgaag gagcattgac cttgacttcg ttgaaacgac
    27301 gagcaagagc tgcatttttc aagatggtat tacgatattc gtcttgagtc gttgctccaa
    27361 tcactgtcaa ttctccacga gagagggctg gcttgagaat atccgcaagc cccttagaac
    27421 cactgtcacc tccagtagat ccagcaccga gaatttggtg aatttcatca aagaagagga
    27481 taatatttcc tacttctttt acttcgttta ctagattttg cacattttct tcaaagctac
    27541 cacggtattg agtaccagcc tcaagacctg agatgtcaat ggaaataatt tccttattct
    27601 taatagcagc aggaacatct ccgttcacaa tggcttgtgc caagccttcg acaactgctg
    27661 tcttaccaac gcctgcatcc ccgaccaaaa caggattgtt cttggtacgg cgtgagagga
    27721 tttcagatgc ttcttgaatt tccttgtttc gtccgataac aggatccagc ttgccctcac
    27781 gcgcttctgc tgtcaagtta cgacctagtt ttgcaaggac accatcttgt ttcatatctg
    27841 aaggctgttg tggcattttt ccatcaactt ctgcatttcc tgccaattgg ccagttgcac
    27901 gatagtgagc aaattcctca ggtgttactt cacgtccatt aatcaagtaa cggcgatttt
    27961 cagaactgta tcctcgcata ccacccatca attggttaaa taaatcatcc atgttgttaa
    28021 aattattaaa gttgttgttc atattcttta cctcttttta tttacttagt tatgattact
    28081 gatattgact atctttgacc tttgttttaa aaaatttaga ttagctaaat cgctactcta
    28141 ggtactattt ctggtttttc tttttcaagc aggagtagac tatctttact tctgaagatt
    28201 tctagattaa acatagaccc cacctctttc tattttactt taaataatac tcttcgaaaa
    28261 tctcttcaaa ccacgtcagc tctatctgca acctcaaaac agtgttttga gcaacctgcg
    28321 tctagcttcc tagtttgctc gttgattttc attgagtata agtgttcgag taaggctttc
    28381 atttacctta cgttcataat atactacatt ggtcagaaaa ggtcaaggat tttgactttg
    28441 attgaccaat gtttttatac tcttcgaaaa tctcttcaaa ccacgtcagt tctatctgta
    28501 acctcaaaac agtgttttga gcacttccta atttgctcgt tgattttcat tgagtataag
    28561 cgttcgagta aggctttcat ttaccttacg tttataatat actacattag tcagaaaagg
    28621 tcaagagttt tgactttaat tgaccaatgt tttttagaat gcaaaaacac cctgaaactg
    28681 aacatcaggg tgccattctt acatcaaata taaaattgct agggtcagta gacttgctag
    28741 aagcccaact ccccaaaaga agaagtcaac cttccactcg ctccaaggat ttcctttacc
    28801 agacaatcct ccaagctcaa aatggtgatg tacaggcgtc atacggaaaa tacgtttacc
    28861 accagtcatt ttgaaataac ttacttgcat cataaccgaa gttgtttcaa atacataaac
    28921 aattccgata atcaagagag tccattcttg gtggagagcc attgaaatag ctgccagcat
    28981 tccaccgaga gccaaacttc ccacatctcc cataaagacc ttggcaggct tatggttaaa
    29041 gacaaagaaa ccgagcaagc caccaatcat ggcaagaatc actagaagaa tatccatctg
    29101 accttgcaca taggcaataa ctccataagc tgacaaacta atcacaacgg aaatactagc
    29161 tagaccgtca ataccgtctg tcaagttgac tgcgtttgaa aaaccgacta gccagaaaag
    29221 agcgaagaca atatagaaaa tccctagatg cacttggtaa ccaaagacag aaagcatatc
    29281 gccaccgcgc tcatagaaaa ggtagaaaac gaccccacct aggagctgaa gagccaattt
    29341 ttgcttagga ttcagcccct cattgatttt acgaaagacc ttgagaaagt catctaaaaa
    29401 tccgacaagt ccataaagta ccaagatgaa caaaatcatt cccacattat tggtcaattg
    29461 ttttgaaaaa agagcaagaa ggaaactcac cacaactgca gcaatgagga aaacaagtcc
    29521 ccccatagtt ggcgtgccag cttttgcctg gtgctgtttg acatcctcat gcatctgctg
    29581 gcctgtaatt tgcgcctttc tataaaattg gataaaggcc ggaattccta ctaaagttag
    29641 taaaaatgtc acaattccag cactaataga aataaacata ttagtctcct aaagttaatt
    29701 taattttttt aatgtttttg atagctgtat tagtccgaac atcttgcttc tgaacaacgg
    29761 aacctgaacc ttcaaattcc agttcaatat ccaaccattt agcaaaggtc tcggcagtct
    29821 cttttttcca gccatacatg tctggaattt cttctacctt atccgataaa aggagaactt
    29881 gttggtttgg tgcaagattg gtcccttctt ctacagaagt ctctttaatc tttgttccag
    29941 tacctacaac gattggttgc acaatatttc ggcgtaaggc ttccgccaac tcaccaggtg
    30001 aaatatcctt gatgctaggc attgcataag aagattctgt cgtaacttta tctaaatttt
    30061 tggctggaga ttgaagattg agagattctt tcatagctga agcccgctcc aagattgggg
    30121 tggcaaattc tcccaactgg atacctgaat aatgctcagg ctgttgaacc gttacataca
    30181 agataaaatc aggattttca gcaggattca tagtcacaac tgagaaaata taattggtag
    30241 aaccaaccaa gtatcctcca tttttctcat cagcgatttg agccgtaccg gatttaactg
    30301 ctacattttg tccaggaact gttataattg gctttcctgt gtagtgatta tacatagttc
    30361 catatagagg gtccgtccca actaagatca tgtgatttcg agttgtgctt gctgcctctt
    30421 tggaaacagg atttcctact atttcttttt gtgacttacg tacagactga ttgttagtat
    30481 cataaatagc acttataaat tttggctcca gcataactcc atcattagca atagctgtaa
    30541 aggcacgaag catttgtgtt tgtgtcactg aaattccttg cccaaatgag ctttgagcaa
    30601 tactaacaat attatcagct ggaagttgac cagcgtattc atctgtcaag ccaaagcgag
    30661 ttggaacccc aaatttaaag cgttttagat aatccaacca agtagcatct cccatttttt
    30721 gttcaagtag actcattcca acattactgg agtgagcgaa accttgtaag aaagtcatca
    30781 tcccaccagt agtcaaacca tcattaacat cccaatctcg agtcgtcgca tccgctattt
    30841 tgaattcact gctattgaag tattctccac ttgggaaggt attattatca atagaagaag
    30901 ctaacgtcat aaccttcatg gctgatcctg gttcatagtt actttgataa agaatatcac
    30961 gccaaacaaa gtcctcagtg attccttctt tagtatctgc attaaaggta ggtcgttggg
    31021 tggtagcgag aatttcaccg gtctttgcac tgaccaaggt cgcggtcata tacttacctt
    31081 ttactttttc tagaaaggca tccatctgag tttccatgaa ggactgaagg gtgctggaaa
    31141 tagtcgtata aacatccttg ccatctacag tttgttggga aacttgttcc gttccgggga
    31201 caatatttcc cagacgatcc ttttcatagg taataatgcc gtctgtccct gcaagaatac
    31261 tgttcaagga actctccatt ccagaggttc ccagcaagct cttgctgcca tcttcatttt
    31321 catggagctg agctagtccg ataaaactag aagcaaattg tccgtttggg taactacgat
    31381 tgggactggt tgtaaaatca atccccttga cctctgcagt ttccaactct tttttgatag
    31441 acatcatatt ggcataggta atcccattgc cctttgctcc aaaggaaact tgcttgagat
    31501 taggttgcga gagttgctct cttacatagg attcttccat gtccagatac ttatgaaaga
    31561 cctctgcaac cttgttaaat tgtgtttttt ctacgtaaag aatcttaccc gttgctgact
    31621 tatagttctc atcaataacc gcatagacat tataggaggt tgcatcctca gcaattggga
    31681 ctccatttcg gtcataaata gtcccacgtt tggcaggaac tgtacgggtg gtttgatgaa
    31741 ccttcttagc ttccttcgct aaatctgttc caaagcgagt gcccgtccca ataatgaccg
    31801 caaaattgac taaaaaaacg gcaaaaacaa agacagataa taaactcaga ctttttccaa
    31861 ctctgcgtct gttttcagcc ggcgatttcc gatttttcgt cgcataacgg attacttttt
    31921 ttgtccactt catatcttac tccgctattc gaatattttc attgtttaat tgcaaatcgt
    31981 gtgaattggc aatttctttc aaacgttctg cacgtaatag ttcattgacc tcttgcttgg
    32041 catcgtccaa ttcggtcttc ttttcctcta tctgcgcatt gatttttgtc aaatcattct
    32101 gcacttgcaa gagcttggtc tgcataaaaa taatactaat ggctacaata agagtggtta
    32161 cagcaatgga aaagtaaaaa gctttttcca cacgcgaaaa ccgtttaagt tgcatctgta
    32221 gtatttgacc tgttttttcc attctttctg ccatcatttt tcctcttact tgtgaatttt
    32281 tctggccacg cgcaacttag ctgagtgcga gcggttattg gcttctaatt cttctgcact
    32341 tggcaagatt ggcttacggg ataccaattc catcttgggc ttgagatcat ctgggatgaa
    32401 gggcaagcct tttggaactt caactgttga agcttcctta aacaactgct tggtcaagcg
    32461 gtcttctagc gaatggaagg taatcactga gattctacca tctagagcca acatctccat
    32521 agcctgctgg atggactcat ctgccgctcc cagttcatca ttaacttcaa ttcgaatagc
    32581 ctggaagatc tgcttggcag gatggccctt cttcttgagc tccttggcag gcttagccga
    32641 cttaataatc tccgccaact cagtcgttgt ctcgattggc ttgacttcac gtgcttgttc
    32701 aatcttacgc gcaatctgtt tagagaattt gtcctcgcca tacttgaaga aaatacgaac
    32761 caagtcatga tagtcatagt gattcaccac ttcataggcc gtcagactag catcctgatt
    32821 catccgcatg tccagtggtg catccttttt ataagaaaaa ccacgctcac gctgatctag
    32881 ttgaggacta gacactccca agtcataaca aattccatca atttcctgaa caccagcttc
    32941 gcgcaaacgt gcctgtaaat gacggaagtt atccttgata aaagtcacca tccccttctc
    33001 aatgtaaggt gccaagcgtt tttgtgcgtt gtcaatggca ttctggtcct ggtcaaaggc
    33061 atagagatgg cctttttcac ttaatttgct taataaatat tcgctatggc ccgctccacc
    33121 caaagtcgca tcaacgtaga taccgtcagg ctttacgtcg agcatatcaa tcgtttcgtg
    33181 gagtaagacc gttacatgat gaaattcttt tgtcatatct tatctatttt accacaaatc
    33241 cgactagctt gcactagtca gacaaataga gcaaacccaa gtaagatttc tttaagaaat
    33301 atgtcaaata tgcttgacat tcactctata gaagaatata atatagtcaa atatatccga
    33361 cacaaatcag aaaggagaag agatgaatcg tgtgaaagaa tttcgcaagg aactgggcat
    33421 ttcccagctc gagctcgcca aggatatcgg tgtctcgaga cagaccatca atatgattga
    33481 aaacgacaag tacaatccaa ccctggaact ctgtctcaat ctcgcccgca gcctccaaac
    33541 tgacctcaac agtctctttt gggaggaaga tttttaaaaa aggagcaaac ttatgaaaaa
    33601 agaaaccttc actgaaaaac tgatcaaacg cacatacggt atttctggtc cccttgacga
    33661 atacaaacgg cgtgaggccg atcgtattgg gaaccaagtc tttatcgtcc tcttttatct
    33721 gatgattttc ggaaatctta ttccactcct tctggcctat aaataccctc aagaagtggc
    33781 tctaatctat cctcctctga ttttagtgat tgccctcatc gctgctggct atgtcaccta
    33841 ccaaatgaaa aaaacaggga ttacagctat tgatccagat atgctgagtg agaaagaaag
    33901 taagcaacta cattacccag gtcttaaagc aggtttgttc tttggtctat ggatgttttt
    33961 tataactcct cttctcagta tactcatagg tgaaggtcag gactattttc attctctcct
    34021 cactataaga aatggtgtat caagcattct cggttctatt ttcttcggag cgagcataca
    34081 gttcctcatc tcccgtcgca ttgcaaaaac taagaaagat caagatgagg attaggagcc
    34141 aactcatgaa aaaagaaacc ttcactgaaa aactgatcaa gcgcacatac ggtatttctg
    34201 atccacttga cgaatacaaa cgacgcgagg ctgatcgtat cggcaatcaa gtctttatct
    34261 tcctctttta tctgatgatt ttcggcaatc ttattccact ccttctggcc tataaatatc
    34321 ctcaactagt ggctctagtc tatcctcctc taattttagt gattgccctc atcgttgctg
    34381 gatatctcac ctaccaaatg caaaaaacag ggattacaac tattgaacca gatatgctga
    34441 atgagaaaga aagtaagcaa ctacattacc caggtctgaa agctgggata gtctatggat
    34501 taatgagttt ttttacaacc cctcttctcc atatactgct aggtgagagt caggactatc
    34561 ttcagtcact tctcgctttt aaaaatattt tttcaagtat cctccagtct gtcttctttg
    34621 gagtggttat acagattctc atctcccgtc gcattgcaaa agccaggaaa aatcaggata
    34681 atgattagga ggaaactcat gaaaaaagaa aagaaacagt tacgttatcc tggtctgaaa
    34741 gcaggttcga tttatggaac agtgatattg tttataattc cactcattga taccctaaca
    34801 agtgaaaatc caaattttat cagttctctt ctaaatacaa aacatatttt taaaactata
    34861 ctgggagctt tctttttcgg agtgatgata catattgtcg actcacttcg tattgcaaga
    34921 gctaaaaaag accaggatta agaggtgccc tatgaaatca ttactaactt tacttaccta
    34981 tcatcttttg ttcagttctc tgctcatctt catcatcgtt tcagggaact taatcagaga
    35041 gatattcctc gtgctagtct ttgttccagc ccttaacaaa gtactgactt atctaaagat
    35101 tgactcccca aaaacgcgca cacttaactt agcactatac tttatgattc tatcttttcc
    35161 acaactgatg ctcgtctcag gcaattggaa ttattattta ctgctgacta tcgctagcct
    35221 ttcttctatc atttatcttt attatctcta tcaattcgta aaagaaaatc agtaaaaccg
    35281 ctcatttagg aggttactaa catgaaaaaa gaagacttaa ccactcgcct actccgaaat
    35341 ctctttcata tccaaggccc ttttgatgaa tgccgacaag agattatcta caaagcttgt
    35401 gcccgttcca tggtccaaat cgtttattct tccttcttcc tcttcttgtt ttatctatta
    35461 tttggacgct tcatagaggc cgttcgctat gccatgccct acgtttactc tggactcatt
    35521 tttttcatta ccttaaaaac tcaaagagtc gtcaaagaac tccatctgga aaaagatgac
    35581 aagtcagaaa tcatcctcaa aacctacagc aaagcccaaa tcaaattccg aagttggatt
    35641 gtgtttatcg gtcttcagat tggcctcttt accttactca tctttcataa agtcttcgtt
    35701 cagcagatgt ccctttcaga ttttgtgaag ttgctcatgc aattcgacaa aagtgttcct
    35761 ttcctaatgt acggcctgat tatcggcagc atttttggaa ccctgaccta cggatttcta
    35821 tcactacagg aggagaaaat tcctaaaaat accaaacaga aggagaaaag caagcaatga
    35881 ctgcactata cgatttttca gtcttgaacc aagacaatca agaaactcca tccaatggaa
    35941 atttccaata ttcctagtcg taaccatcat ctcaagtttg gtctacttct ataactttta
    36001 tcaagtagtt aaagaagtaa atcaaaaaca gttgatttag gagattactc ccgtaatttc
    36061 tcattttata gacaaataat cccatccagt tcatgctaga tgggattatt ttatttctta
    36121 acagaggttg ccagttattt ccctttcatc ttagaaagtc gactctttcc aactcacact
    36181 acaatccaag acttgttgcc tttcttcttg attgaggcct tcaatctggt caatcaaaat
    36241 ctttgtagcc tgttgtcctt cttcaaaggc gggctgaacc aacgtcgaaa cacttggaga
    36301 agaaaagcaa gtccactccg tattgtcaaa accaatcaac ccaacttgtg gcaagttata
    36361 attcaactct ttgataacgg taaagactgg aggtagggcc caacagttag gaacaaatac
    36421 cagagttttt tcatcgggat tgatttcttt ttgtaaaaat tccttaattt gttccaaatt
    36481 cgtatgctta tcttcaatgg ttagcctagc gtggcgcata ttagcatctg ttaaagcatc
    36541 cacaaaacca cttgcccgct caatccgagt actcaaacga ctcgtatccg ctgtaatcaa
    36601 gagaaaatgc tcataacctt tttcgataca ggactgggtc atatcataaa cggcatcata
    36661 gttattggtt ttgacccagc tagtccggtg ttcatagagc cgactatcaa aaaagaccat
    36721 tttcttcttt ttctcatcga tgatacgaga atattttcgg aaattagagg taggctgaat
    36781 aataaagccg tctactccca agagaagcat gctttcaata tacctgtcct cactctcttg
    36841 gctgtagtta ctatttccta tcattacctg gtagccattc tggctggcga tatcctcaat
    36901 tcccttaaca atttgggttg agaaactgtt ggtaatatca ccaatcaaaa caccaattaa
    36961 ttttgttcgt ttggagttta agctacgcgc aacaatgctc ggtttgtaat ttgtttcatg
    37021 aataactttt ttaatctttt cacgtgtctc ttgggacatt ttttcatatt tcccgtttag
    37081 gtaaaatgac acggttgttt tcgaggtctg agccatttct gcaatatctt ttatggtcag
    37141 tttcttctcc aatctaaaac acctcctact aatacccatt ataacatatt cactattcaa
    37201 aacgtctatt tctagcactg caagcgaatc attctctcat ttattttatc atatactaga
    37261 cattttccac gcatttttgt tccgtcaacg aggcataatt tttcacctac acaaatatcc
    37321 tcgcttaata acagaatcag cttgtctgtt tcgtcatttt tcacttcaaa agccagagca
    37381 ttttctactt gatgaccatc tgtctggtag acagaatgac ggatgatttc aaaactctca
    37441 tgcgcaatga ttgtataatc cagcatctga ttctcaaaga attggtgttt gatgagtttg
    37501 ctactttttt caagctcgtt gtatttagga gaaatgatcg tagcttccaa atcaaaatca
    37561 acttcactcc atagtttcaa ctgattgatt ttcccatctt gataggtcac atccttgtca
    37621 aggataaact gagtcaacgc ctcatgctga ccttgacacc tgatatcatc taccaagagc
    37681 catacatcct ctgccaacat gaggattttt ctcctgtgaa gataaggcaa atcaggttct
    37741 gctgacaaat acgccccctc aatataatgc actccctctc tttctttgtg gtgacaaaac
    37801 agggagtgag gatagtattc atattcccag gatcccacga tcctttctgg agctttccca
    37861 tctactatac aggtcgaatg actccaagca ctctttaaga gataacgttc atctacctcc
    37921 cgataagaat aacgcccagt atctatgaaa atcggttggc cttgatactg taagcaaaaa
    37981 ctattctcgt cactatggct atgggcactt cccagcggac cgtttttaaa aaatagataa
    38041 cgatgttcat ccttaatgca aacatgccca gaatcttcaa agctcctgaa cttaggctgc
    38101 caagctctct tttcaaattc ctgcagtcgc ttgacctttt ctcgccccag gaacaggagg
    38161 ctaagtaaat cgactttaac atccagaccg ttaagaaggt cttccttgtt caaaaccata
    38221 gcagacaggc tcaaaatctc tgtcgtttct gtagaatcgc tatcaccaaa agccaaggtc
    38281 cgtccatcta gacctgtcat catttgaatg taagtcgcca tcttttccag cagctcttga
    38341 aagctatctt gcaagtccgg aagcaagaga cacaagtcca gcaaggcttt ataaacctct
    38401 acatgataga gaatcgactg ttcaaactga cttccatcct ctaaaatctg cgtctcgatc
    38461 tgctgtttca actcctttga agcaaaatgg taagcttctt ctaggtccat cttatctgaa
    38521 aagaaatgat aggttgcaag tatcggaatc gtttgtaaaa ttccccagtt actaagggtg
    38581 tacttggcgc gataatggct tttcataaag tcaatctgct tttctagact gaccaaaatg
    38641 ttctctagtt cttcctcctc tagcaggtca aatttcaaga ggagcaagag tagtttcaac
    38701 caagtaaagg aacgaatacc cgtatccaag gttctagtca tcaaagattg aggacgaaat
    38761 tctctcacct gctcaatcca atcaaataga aagaacttgc acttttggat atagccctta
    38821 tctccttcta ccaggtaccc tatcataaac tgcaagagat attcttgtcg attgagcata
    38881 taagcccatt ctggatcatc ttcaaatact tgattccaca ccatcggctg gatttgatgg
    38941 atttttgaac aaggttccat atcccaagga ctatcaaaca taaaacgatt gtccatcaag
    39001 cgttcaaggg aacgcttgac tttctcatag tcttgtgaac agtgggacaa gatataatca
    39061 cgatattgat ccccatcaat tttttcaaaa aattgtcttc tttcttcttt cattatctat
    39121 taccagaaaa agaactactt aaaaagcagt tcttttgtct ttcccattac actttccttt
    39181 tctacataga tgaccacacc ttttgcaatc tgcaaggaga ccaagtcatc tttgatagaa
    39241 atgatttttc catgaattcc agacaataac aacacttcat caccagatgt taaagaagct
    39301 aaatactctt ttcgttgctc catctgtttg cgaagcaact tttgctgacg aatagaatga
    39361 aagcttgaca gtaaaagggg gctcactgcc aagacaatca ctattccata aaacaatgtt
    39421 gtatccatta agctagaatc ttaagccagc ttccgataat tccgatgata actgttaaaa
    39481 taacgagttt atatgttgtc catttctttt ctttgatcaa gtagtaaact aaaagtgtaa
    39541 atagggctgg tagaagagct ggagcaacct tatcaagcat tccctgaata cttacgatac
    39601 tttgtttggt atctgcttta acttcccctg cagcaaaggt gattggcacc ataatcttca
    39661 cagatgtcgc tgccaaacca gcaattacgg ttacaccgat aatattagca atacgagaaa
    39721 tcgttgccat ctgttcactt agtttatcaa tcacagtcgt tcctagtttg tatccataga
    39781 gaccagttga caatttaatc gctgtcaaaa tcgtattcat cgctaggaag aacaagattg
    39841 gaccgacaac caggccttct tgagcaaaag aagcggcgat ggttgagaac aatggagcta
    39901 aacagaattg agaaagagaa tccccaatac ctgccaatgg ccccatcaag gccatcttga
    39961 tgctacgtgt ctcttttgcc ggacggccat tttccaacat tacaaggtgc aaactggtaa
    40021 taaaaggcag gaagtgtggg ttggtattat agaattcaca gttttcttcc aaggcttggt
    40081 agaaaacttc ctgatcctct ccatagtgtt ttttcaaagc aggatacatc acattggcat
    40141 atcccaaccc ttgatagtta ctatagttaa atccattttg acaaaagaat gcccgcaaag
    40201 acgttttaag ataatcacgt tttgttaatt tgttagatcc agtcatcgtg tgcttcctcc
    40261 tctaccacat gattcgctgt ttttggcttg ttataaaatt caatcaaatc aaagcaaaga
    40321 tagtacctac aattgcaata ccaattgttg ggatgtttag ataagctgca caaacatatc
    40381 ccaacaagac aaagggaatc aactctttct tagccatcac tgacaagatc atcgcaaaac
    40441 cgatagctgg gagcatttta ccagcaactg tcaaacctgt aagcaatacc ggtggaatgt
    40501 agtctacgag tttcaacaag gtatccattg aaagggcacc caagcaaccc aaggtaaatc
    40561 caataaaggc aaataaccaa attgttgcat ttagagtgaa cttgaatttc ttcaaattat
    40621 ggtttttcaa gtgcttcatc gccgtttcag gcgcaccagc acgtacagtg taggcgaaag
    40681 tttgtaagaa ttgaatcgcc acagcaatcg gagtagagag ggcaagagcc gcttctggac
    40741 tgactttacc agcactagtg atagccatca aggtaccaaa gataccaggt ccgattgggt
    40801 ttggtggaac agtacctcca gcaccaacac cgaatcccat ataagccaat tcaccaatag
    40861 ctcccattgc aagagcagta ggcaaatcac ctagaataat tccgacacca aatgacagaa
    40921 caatgcatct attggtgtaa attcctaaca gcattccact aaaacagaaa gctgtccata
    40981 atccaattaa aatcgcttga aatacattaa tcgacataac gatctcctct tttaaatata
    41041 gtccataatg ttgacttcaa cagctccatc atttcctgtt ggagttgttt tcgtattaaa
    41101 tgttacttga taagtttggt tcaattcctt gagagctgcc ttgtcctctc cacccaggaa
    41161 gatagagcgt gtcacttgct ctttaccagg ggcattgtga atgttcccaa tattgatttc
    41221 tttgataggg acaccacctt ctaccaaggt taaagcgtcc tttacatcct ttacaacgat
    41281 aaagatcgtt tgagcaggat tagccttgtg aatgatatca atcacctttt gcaaagggaa
    41341 gaaacgcatg gcaactgagt ctggtacaac tgttttcatc agagtttgtt gcatcttgtc
    41401 cgtgcttact tcatcattgg caacaatgac cgtattacaa cctaggtatt ttacccaaag
    41461 ttgtccttgc ccatgaatca accgttcatc gatacgggtc ataataatat ttggcattgt
    41521 catatttctc ctcctaccaa tataggttcc aatccttgta gaaacggata agggcttcta
    41581 ggtaatagta gtcaccccag atattgcctt cgtccactcc tttacctgaa tgccatgagt
    41641 acacaccgtg gaggagactt gtcccaccag gggtaaattg atcatttgca taatgttcga
    41701 tcaaagaacg aagcatggca tgcatagcat gtttataaat atctttgtca gcatccacct
    41761 ctgggagatg ttttagcatt tcatgaatcc cacagacagc gatagctgtt gctgaagaat
    41821 ctcgtgattg atcactacca tcattaaaaa tcaaatccca ataagaaaca tgatcttttg
    41881 gcagacgatt caagaaataa ttggttacac ccttaaacaa gtcaaagcag gactcgtctt
    41941 tcaggtgacg ataagtcaaa ggaataccat agactcccca tgattgacca cgtgcccagc
    42001 atgaatcatc actataccct tgtctcgtta cacctttaag gggttgacct gtctcaggat
    42061 caaagtagaa ggtgtggaag gacgaagcgt catcacggat tacattatta gctgaaacat
    42121 agaaatggct ttctgcaata tcgtagtatt tttgatcgcc tgtttcttga taagcaaaga
    42181 ataagagttg gatgttgagc aagcagtcga taatcaaacg gtaatgctct ttcttgccca
    42241 agtctcccca agcttgaata aaaccacctt tttcttgata gcgttcaatc aacttatctg
    42301 cagctttcaa ggctgcctct ctggcctctc catctccatt tatcttatat tcagccatac
    42361 aagacggtgt gtacaagaag ccgagatcat ggtgatccaa ttctactctc ttattgacac
    42421 gatccaggaa agaaagaaca tttttatgag cgatgttttt aaattcatcc tgttgactgt
    42481 attcataagc caaccacagt tctcctgtcc agaaaccatt ggtccattcc gtattatcca
    42541 tgattggata gacattatca aaggtagctg gcgtcgggaa atcttccttg aaatagtcaa
    42601 ggttgagttc taactgccga ataaccttat cgattgcctg gtcgacttct tctttcgtca
    42661 gaagtggtac ttctaagaag cgctcaggcg attttatttc ttcaatcgta acctttttta
    42721 tcattctttt accctttaaa tacccgattc gaaatcttct tcctcttcct ctgcatccgt
    42781 tgaaaacaat tctttaccat ttacgactcc gccctgagcc gccactactg atttattaac
    42841 aacctcatca aagctatgag ccattctagc aaagactgct tccattaaca tggctaagtt
    42901 caaacccgag agaacattca ttgttttggc tggattttct cccattatgg tagaagaaac
    42961 cttgaatggc gatcctccca agagatcact taggattaaa acttcttctt catttgaaat
    43021 tgcaagtaag attttttgct tgagttcatc tgctgacatt ccttccacaa agtcaatcgc
    43081 ctccacattt tcttgattgc ctgcaatcaa ttgtaaagaa ctataaatcc ctgtggcaaa
    43141 atgtccatgc cctacaagta caattttcat cttttctcct ttcttttttg agtttaccgg
    43201 tttactctat attttacatt gtagaccttt atttttatct tgtcaatagt tttttcaaaa
    43261 aaatattagc gctttcattg cttatttaac cattacaata gacagaaact tcaaaatctt
    43321 ccgttttttt aggaaaacaa agattttcag aaataaaaat atttaatcat taacaaaacc
    43381 ggtttactag aatataattt ggttatagag aagtcttaac aaatttgaaa ggaagtatca
    43441 aatgacaaat acatcattct caattgaaca gttttcttta aaaggaaaaa ttgctctcat
    43501 caccggcgct tcttatggaa ttggatttgc tattgccaaa tcctacgctg aggtcggcgc
    43561 tactattgtc tttaatgata tcaatcaaga cctggtcaat aaagggattg aagcttatcg
    43621 tgaagttggc atcgaagccc atgggtatgt ctgtgacgtg acagacgagg acggtatcca
    43681 agccatggtc aagcaaatcg aacaagaggt tggtgtcatt gacatcctcg ttaataacgc
    43741 tggtattatc cgccgcgttc caatgtgcga aatgagcgcc gctgatttcc gtaaggtcat
    43801 cgatattgac ttaaacgcac catttatcgt ttcaaaagca gtcattcctt ctatgataaa
    43861 gaaagggcat ggaaagatta tcaatatttg ctcgatgatg agcgaactgg gacgtgaaac
    43921 ggttagcgct tatgctgccg ctaaaggcgg cttgaaaatg ttgacccgca atattgcgtc
    43981 tgaatacggt ggagccaata tccaatgtaa cggaatcgga ccgggttata ttgccactcc
    44041 tcaaacagca cctcttcgtg agttgcaaga agatggttct cgccacccat ttgaccagtt
    44101 catcattgca aaaacacctg ctgcacgttg gggaaatcct gaagatttaa tgggccctgc
    44161 tgtctttctc gctagtgatg ccagcgactt tgtcaatggc cacatcctat atgtagatgg
    44221 cggtatctta gcctacatcg gaaaacaacc tgagtaaaaa tagaaagaag atcttatgaa
    44281 aatcgcatta atcaatgaaa atagtcaagc tagcaagaat cacattattt acgatagtct
    44341 aaaagaagcg acagataaaa aaggctacca attatttaac tatggtatgc gtggagaaga
    44401 aggtgaaaac ccattaacat acgtacaaaa cggcctaatg gctgccatcc ttttaaatac
    44461 aaaagcagtc gactttgttg ttaccggctg tggtacgggt gtaggagcta tgcttgcttt
    44521 aaacagcttc cctggtgttg tctgtggtct agcagtggac ccaactgacg cttaccttta
    44581 ttctcaaatc aatggtggta acgccttgtc tatcccttat gccaaaggat ttggctgggg
    44641 ggcagaactg accctcaaat taatgtttga acgcttattt gctgaagaaa tgggcggtgg
    44701 ctacccaaga gaacgtgtag tccctgaaca acgcaacgct cgtatcttaa acgaggtgaa
    44761 acaaatcacc cacaatgatt tgatgaccat ccttaaaaca atcgaccaag acttcctcaa
    44821 agacaccatc tctggcaaat acttccaaga atacttcttt gaaaactgcc aagatgatga
    44881 agtcgctgct tatttgaaag aagtattagc caagtaaagc cattctaaac cagaaaggaa
    44941 ctaatggatg acgaaaatat tactgtttgg cgaaccatta attcgaatct caccattaga
    45001 tgccaccagt atcggagatc atgttgccag ttcgacttat tttggcggat cagaaattaa
    45061 catcgcttgt aatttgcaag ccctgggtat ctcaacgaaa gtctttaccg cactccctgc
    45121 caacgagatt ggagatcgtt ttctcacatt cttgaaacag caccaaatcg ataccagttc
    45181 aatctgtcgg cttggctatc gaatcggcct ctactatttg gagaacggct ttggttgtcg
    45241 tcaaagtgaa gttttctacg atcgtaagca tacgagtatc agccagattc ggccaaacat
    45301 gctagatatg gattctctct ttcaggggat tagccatttt cattttagtg gaatcaccgt
    45361 agctatcggt caagaggtcc gtacgatcct tctcctactc ttggaagaag ccaagcgccg
    45421 aggaattgtc gtttcaatgg atctcaatct gagaacaaag atgatttcag ttctagaagc
    45481 caagtatgaa ttttctaagt ttgtacgttt tactgactat tgcttcggta ttgatcctct
    45541 catgattgat gaccaaaatc tagagatgtt tccaagagac agtgctagcc tagaagaggt
    45601 ggaaaatcgc atgcgacttt taaaagaagc ctatggtttc aaggccattt tccataccct
    45661 ccgctctagt gatgagcaag acaaaaatgt ctatcaagcc tatgctctgg aagaacgatt
    45721 tgaagagtct gtccaactaa aaactgcagt ctatcaacga attggtagcg gggatgcctt
    45781 tatatctggt gccctttacc aactactcca tcattcctcc ctaaaaacta ccattgactt
    45841 tgcagttgcg agcgcaactc tcaaatgcac tcttccagga gaccatctct ccacttccgc
    45901 aactagtatt gaaaatttac tggcaaatgc acaagatatc attcgttagg agaattacat
    45961 gaccaaatca gatacgatta ttgaactaaa aaaacagaaa attgtcgctg tcattcgagg
    46021 gaatacaaag gaagaaggac tgcaagcctc gattgcttgt atcaagggcg gtatcaaagc
    46081 tattgaaatc gcctatacca atccgtatgc aggacaaatc atcaaggaac ttgtagactt
    46141 gtatcaggac gatcagagtg tttgtatcgg tgcaggtact gtgcttgatg ccgtaactgc
    46201 tagagatgcc attctagccg gagcaaatta cgttgtttct ccatctttcc atgctgaaac
    46261 tgcgaaaatg tgcaatctct acagcacacc gtacattcca ggctgtatta ccctcacaga
    46321 gatcacgact gcacttgaag ccggtagtga aatcatcaaa ctcttcccag gtagtgctct
    46381 cagtccagca tatatctctg cagtcaaggc accgatccca caagtttccg taatggtaac
    46441 cggaggagtc ggtctaaaca acatccctca atggttcgct gctggtgcag acgccgttgg
    46501 aattggtggc gaactcaata aactcgcttc ccaaggcaac tttgaccgca tcagcgagat
    46561 tgcccaacag tatgttacac tcagataaaa tcataaccac ccgtcaaaca ggtggtttat
    46621 cccagagcta taagcccaaa ttaccagcca gcgcctaaag acgctggctt tcacgttgtt
    46681 caagccttat tgctcttgac tcgtcacttg cctctttaag agactttggt attacttacc
    46741 actatcccta aagggatcct catattcttt tacactcaat ttatctagtg ctatagtaga
    46801 ttgaaactga aatagtacac ctctacttct aaaacattgt taaaaatcga tttgactgtc
    46861 ctgatcgatt tgttctgttc ttatttcatt ttactatata tcatgcttta ctcgttctca
    46921 aattttcata ctcgtgaaga aatcatccac tggataattt ctttaatctt gactatattt
    46981 cttaattgtg gctttattaa gccctactgt acttacataa taaccttcca cccagaaatg
    47041 ccgattccca aacttgtact tgagattggc gtgtttgtca aacatcatga gtgcactttt
    47101 gccttttaaa taacccatga aactcgaaac acttagcctt agtggaatac tgactaacat
    47161 gtgtacatgg tctggcatta agtgaccctc gatcatttca acacctttat aactacacaa
    47221 gcgatgaaat atttcgccta aactacttct atattgatta tagatgactt ttcgtctata
    47281 cttaggggtg aacacaatgt gatagaacac ctccactttg tgtgagataa actatgagcc
    47341 ttttgtgcca tattttttct cctttcgctt tacaattgga ttgaacacct ttattgtatc
    47401 gcgtttggag tttttttggt ataaccttcg acgcgcaccc gcatagcggg tggttgtttt
    47461 gtctcgcacc tcacggagcg agacggacta atatagtgga gtgaaatagg atacgaacaa
    47521 attgattagg aaaatcaaat gaatttatag aaatctttta gcagttatga cgtactattc
    47581 tagtttcaaa acgctatagt cacataatga agtaaaaaag gataagtatc aacttatccc
    47641 tttttaaaag aaaaatccga agatatttgg ccttcttcgg attttttcta ttttccacag
    47701 tttcatgtaa ttcatctaga tgatgaacaa attagttgtt ctttcctcta cggaatagat
    47761 aaaacgcccc aagtagcaag aaccctagac ttgccaagat tgactgacct tctcctgtct
    47821 gagggagatt cttttgatcc gaatggttct tttcctcttc agatttttcc ttttcttttg
    47881 aattctgtac ttgtggctga gctgcttact ctagcttttt aaagacttcc tgatctggag
    47941 ctgattcctg ggtttcagga ttatagtagg caatcttata ttcatcccct tcttttcgaa
    48001 tggtatagac tccacgtttc aaaacttgga attggttgga aatagtagag acagaatcat
    48061 catatttcac aatgccccaa actccttgtt tggcatcata aacagactga agggtttcgt
    48121 tattttcgat gaggctactt tctaactctt ttatcatttg attgaaggtg gcacgatcca
    48181 cgttagggat gagcatatag ccataagaat ctccattttg cttatgatcc tgactaatcg
    48241 taagaaattc attttcaact tccttgtctg actgtccttc attgatatcc ttccaggctc
    48301 ccttttgcaa agccttactc atactgattg aactcttctt aaagaaaaag taaccaatat
    48361 tctttttcga atcggacgat tctaaaaaga cactttgggt ttcaggataa tccttttctt
    48421 gttctgtaag ggaggcttct ttatcattga catagacttt atatggatta cttgattcca
    48481 gttttctctg gtcaattgta gttgcagcag tatctgttga agtgttttgg atattgcttc
    48541 ctaaaaaggc gatcttatcc ttcagcataa accagctctt atgagcagtc aatgtttgat
    48601 tccagttggt gaaatccatg gttgctgtcg cattggcatc atctagtttg ctcgttccaa
    48661 cgaaagcaga cggtagaact ttacctgtat cgctatccgc tctctcagca tccgtctctg
    48721 ttgtaccagg catcttatat ggattgactg ttggccagta gccatcgcta tagtgactca
    48781 aatcgccatt gtaaagatag aacatcacat cactcgtata ccaaccacgt ttattttcct
    48841 tgttcatgtg ttcgtaattc aaggtacgac tggaaaagag tgacaagcca aatccaaacc
    48901 ctttctctgc attgtacatg gctgttttat ccatcttgtt aaaggcagat aggtaacttg
    48961 ttcttggaac acttgcgact cctgcatcac ttaacaagga ttgcatcaaa ctgatatcct
    49021 tataagtctt caaattctta aagacatcat aataactatc cgattgaaca atggtcttca
    49081 caagactctg caaacgttgt ttggtttctc cttcagacat atccgctatt cggtgaatcc
    49141 ctcttagtac ttctactgcg gccacgtgcc cctcgctatt tgcacgactg atcgagcgtc
    49201 cacgactcat atccatcagc tctccattca ccagcaaagg agcaaacgat ttatcaatcc
    49261 agtggtacat ggtttgcatt ttatctttat cgattggatt cttggtcttt tgaatgactg
    49321 gcaacagttg agacaggcca tcaatcaaaa cattcccata agcacccgta taggcaacat
    49381 tggtgtggtc gatataggat ccatcttgat aaaaaccttc accttggtct accaacttga
    49441 acacttgctc aatcgagcga atggtagaag aaatttcttg atcatcctta cgcagtaaac
    49501 cagctattac ttttaccctt cccatatcaa ctaagtttcc acctagagcc ttaaatgggt
    49561 tatcagtcgt ctttcggaaa tgttcgggat ctggtacaaa tttttcaatc acatctgtat
    49621 attttttaat ttcctcatca gagaagtatt ctttcatcag agacaaggta ttgttgatgg
    49681 cacgaggtgt accgatttca taatcccacc agttcccaac aatgctcttt tcactattgt
    49741 agacatgttt atgcatccat tccatggaat ccctgactgt tcgaacgaca gtttcatctt
    49801 gataataacg agaagaagga ttggtcactt gcttggccat ctcctccaat ttccgataag
    49861 tggcagtcag atttgcagac attttataat ttgaaaattt ttcccacaaa taggtgcggt
    49921 ccgcctgact tgaaatacta gataggctat cagctacctt tccttccaat tcctggttta
    49981 atttggccat ctgttcattt ttagaatcat agtattgatt cccagcgatg atgccagtcc
    50041 agtcatccaa acgggctgtg tatgcatcct taacagaggc tagaatcttc aaaggaatct
    50101 ttttcacttc cttgccatct ttactgacaa tgacattggt tgtcccttcc ttaagaggtt
    50161 ctaaaattcc atttttgact gaagcaacgt caggattttc taccttataa gtatagtccg
    50221 caagagaaaa aacatgtttt tttccaattg gtaaatcaat cttttcctca agctgtttat
    50281 ctgtttgaga atcctcagaa agctggtctg ctacctctac cagctcaata tccttaaagg
    50341 aaacagtccc agttcctgtt tcatagaata actccagctt gattttatca acatctaaag
    50401 tcgggctata gtctgcttca atggtctgcc agtcctttgt tcctgacgtc gttgcagaat
    50461 tccacaatcg cttgtcctta ccactttcct caatgatacg aactttggca atcccgactt
    50521 tattatctgt tttaatcttg aaacgcagtt tatacttttt cttagcttca ataggaacca
    50581 tacggtgaac cgctgccctt aatttctcag ggcttgagat agtgatagcc ccatccttag
    50641 cctcaatgac tcgagttgag gcatctgcag aactattctt ctggtctacc caagctgacc
    50701 acccctgagc tttgcttcct gtccacggac tgcttccgtc ctccgtctga ccaaaatcac
    50761 cattttcaac taaatttttt gtatcccttt actgatactg aacttccttc tggctttgct
    50821 gaatagtatt cgtagtcgtt tcttccgctc caactgtcat ataatggttt aataaaaatc
    50881 ctgataaaac aagtgaagac aaactcacaa tgagcttctt tgtttttgtt tgcatgtata
    50941 cactccaata aacatattgt aaaatcaata ggaactaaat ctcaaattag agatttatag
    51001 caactaagta aatcggttta attaaatcat accattttta aagttatttg taaacggttt
    51061 ctatcatatt gagggcttat tcttcaacaa tatcttccat ccgactcgac gaaaaaagta
    51121 aaaactgtta caatgtagaa aaagaaaaga ggccctcatg acttcacttt atgatttttc
    51181 ctttttgaac caaaacaacc aagcaactcc cttggatagc tatcgtggca aagttctctt
    51241 gattgtcaac actgctactg gatgtggttt aacgccccag taccagggac ttcaagaact
    51301 ctatgaacgc tatcaagatc agggctttga aatattggat ttcccttgca atcagtttat
    51361 gggacaagca cccggcagcg cagaggaaat caacgccttc tgtagcctac attttcaaac
    51421 caccttccca cgttttgcca agattaaggt caacggtaag gaagcagacc ctctctatgt
    51481 ctggttacaa gaccagaaat ctggcccact aggaaaacga atcgaatgga atttcgctaa
    51541 gtttctcatc ggtcgagatg ggcaagtctt tgaacgcttt tcttcaaaaa cagacccaaa
    51601 acaaattgaa gaggcgatac aaactctact ataattcaca atctcactat gattaggttt
    51661 cctttaacct gatgaatagt gagatttttt gattggcttt gacttaaata gaaaaacacc
    51721 ccatgatatg aaacatgaag tgttgtaaag tctatgttgt aggtgcttat ttcacatttt
    51781 caatgtgacc agtgataacg aataccatac agaatcttca tatacactaa acaaatgact
    51841 ttctaattat ttcaattagt tttggctagt aaatatcatt tccaacaaac gccctctcaa
    51901 ttccttatcc tgatgatgca agatattcat taagtcatga gagtttttcg cattgatgaa
    51961 ttgatttaac aatctatctt ttaattcata tggaagagaa gctgtcttta gtagtctaaa
    52021 aacttcgtca tttaaagatg tccttttatt atctttccat tcaaatttag ctgtatcatt
    52081 cttatttggc aattcaatta tagacacatt cgttccttta aaatgaattc tatgttttct
    52141 attgcttgga acgatactag aatctccttg taatgctaac tctaccattc ccatttccca
    52201 atcgattgat aatcttgttt tatatctttg accattttga tcttcaagca tttcaaaaga
    52261 atgttgtttc cctgggaata cataccaatc tacaacctca ggtaaatcaa cacccatacc
    52321 tatctcagaa ccaaccaagg gaatgattgc accacttttt gcaaacacag gcgtagtcga
    52381 gatgtcccta taaacactta acttcacacc acctgtgtat tttttctctg aaaagaagtc
    52441 ataccattca ccttcaggga accatacatc tacttttgca gattggaatg tcaaatccat
    52501 cttttctaca atgggagcca ccatcagttc tgttccaaaa aagtattggt ttggaacatt
    52561 atagctctca tcattctctg gatagaaata atagattgga ctgattaatg gggcaccttc
    52621 ctcatgtgtc tttacattca tggtatatag ataaggaatc atctgatgtc tcaaacgaag
    52681 gtatttcttc ataatcttag atgttgtttc tgaaaaaaac caaggttctt tactattaaa
    52741 aggacttcta gaactatgta atcgagtaat cggactaaaa acaccaaact gtagccatct
    52801 agtttgtagc tcttcgtcat aatcccccaa catatgtcca ccgatatcat gactccacca
    52861 actataaccg atattagatg ctgtcgctgt aaaatagggt tgaaatctta aggaattcca
    52921 actaataata gtatcccctg aaaaaccaac agggtagcag tgactaccag gacctgcata
    52981 tcttgataaa atcaaaccac cttctgcatt tttacaacta tcctgatagt gataatggtt
    53041 taaaagccaa agtggatcta gcataccttg tgtcccttgt tgccagtcaa tccaccaaaa
    53101 atctactccc tgcttttcta gctcataatg aacatcttta aagtaggctt ccctaaaaga
    53161 gggattaaaa aaatcaaaaa tagcaggttc ttctagttct acatttaacc ccaatcgttt
    53221 tgcgatttga ggataagctt cttcataagc ccgtatccca tcagcaggat ggacatttaa
    53281 ggagagtttt aactttctat catgaagttg ttgcaataac tgttctggat ttggtattaa
    53341 gtttctattc caactatatc ctgtccagcc acttccaaaa cgagctggaa tgtcagttat
    53401 atgccaatcc atatctaaca caccgataga taatggaatt ttctctgttt caaatctgtc
    53461 tattaaatcc aagtattcat ccgacgtata aggccaatat ctactccacc aattgcctaa
    53521 agcatatctt ggcaacaagg gtgttgaacc agtcaaatgg taaaagtctc tgattgctcc
    53581 tctataatca tgcccataag caaagaaata caggtcaatt tgattttctc tctcaatata
    53641 accagattgt tcatcccaaa taaatccttg agaatcatcc aataaggcta taccatttcg
    53701 gctaataatt ccatcttcta acgagattgc tccatctgcc ttatccagag tccgagctgt
    53761 tccttttaac gtttcaatag attcaccaaa ataccagcga ctaccatata cggcaaaatt
    53821 tccttttaat tctataaata aattttcggc gttaaattct cctttattaa agtgcagatg
    53881 aaaatagtcc gtcataatat ctagtacatt tgatgtctcg atataatcta acgaaacttg
    53941 gccaaaatct ctattataga taagttgtgt cgttctatcc tcaaaatttc cagtttgaga
    54001 gtattctaac cttactagct tgtctgttaa tacagagatt cgataaaact ctcccttaaa
    54061 aattttcaat ttgttttcct ccttttatgg tagcataaaa acagaacgca ccatttttga
    54121 tgcgtttttc attattctga atgcaatgtt ctatctgtta tatctatgac aaataatagt
    54181 caattgaaaa aaatgtagtg gacaaaatat cttttaacaa accaagagtt tattaaagag
    54241 ttatcacttt tcaacttttc taagcttatg cagttgtgaa acaaactact tttaaactat
    54301 taactaagat aggattgata aataatttca aactcttact agcaatcata cgatattcaa
    54361 gctcgcgtgc ttttttcctt cctgcttatt tcttagaact gaagaacccg gatcggtata
    54421 taaattatcc ggatcaacat agtcataaga ctcataacag ttgcgcttca ttaagtcatc
    54481 cccagagcaa gagcttcatc tcgtaatttt tcaacatcac taaccgtagg tcgccatcct
    54541 tcaatcatat ttgtacttaa agcataccaa acactcttaa aaacggatcg gttttcaaaa
    54601 gctattccca tgattgtcat cttttcttta tctatatcta aggacatatg ctacctcctt
    54661 tagatacatt ataccatgtt tctctgtagc ttttaaaaat tttattttgt ttgtcatatc
    54721 taagttttca gtacgcttat cctattttat aagcctcaaa cccaaatata aaacgcatta
    54781 accatctgat aatatgcgtt ttattgtgga aacatttccc attttctcta tttctaatga
    54841 cttacttcaa cttctgactt catggaaaaa acaacaaaaa tatgaactgg caaagtttgg
    54901 tatcatcagt aatccagaac aattcatctt tacttacata gataccaaag gcaatatcaa
    54961 taaaccttta cattctgact atctcaataa taagatgaaa actatcagaa aacgacaccc
    55021 agagcttaca cacgctacgc cacacaaact acgtcatacc ggagcaacgc tcgctaaaca
    55081 agcaggaatg agcttagaag ccatttctga agctctaata catagcgaca caggtacaac
    55141 acagatttat gtcaatactt ctaatgtagt ccctatggcc gtcggtgaat ttgccttaca
    55201 gtctctaaaa caataaggtg aaaataaggt aaactttgag gtgaactttt tcaaaaaaca
    55261 taaaaaaagc acccatgagg taaactcttg agtgctttga aacgttgata taattgtatt
    55321 gattaacgtt ttgaggcaac tgaccttgtc aggttgcagc cacatttgta agcgactaaa
    55381 gtcgcaacaa ctgtgtcaat tgcaccaatt tagtaagaaa gtataaaaaa agaacacccc
    55441 gaaaggtgct ctgtataagt atagtaattc tttcgaatta acgtttacta aattgtgatg
    55501 ctttacgagc tttcttaaga cctggtttct tacgttcaac tttacgtgag tcacgtgtaa
    55561 gaagtcctgc gcgtttcaat gaatcgcgga agtctgggtc tacttgaaga agggcacgag
    55621 cgataccgtg acggatagct cctgattgac cagcgtatcc accacctaca acgttaacga
    55681 aaacgtcgta tgaacctaca gttgaagtaa ctgcgaatgg ttggttgatg acaagacgaa
    55741 ggtcagcgtg tgggatgtac tcttcaacat cttttttgtt aacagtgatt ttaccagttc
    55801 ctggaacaag gcgaacgcgt gcaacagcgt ttttacgacg tccagtacct gcatattgtg
    55861 cttgtgacat actttatttg ttcctttcct tagataagtc ctgaaatgtc aagaacttct
    55921 ggttgttgtg cagcatgagt gtgctcagct ccaacaaata ctttcaactt cataccttga
    55981 gcgcgtccaa gtgtattgtg tggaagcata cctttaactg atttctcaat caaacgtact
    56041 gcatttttag aacgaagttc acctgcagag atttgtttca atccacctgg gtggtttgag
    56101 tgagtgtagt agattttatc agttgctttt ttaccagtca atttaacttt ttcagcattg
    56161 ataacaatca cgaagtcacc tgtatcagtg tgtggtgtaa atgttggttt gttttttccg
    56221 cgaagtacgc tagcaactac tgcagaaaga cgtccaagtg gtacatcagt tgcgtcaact
    56281 acgtaccatt tacgttcaac ttggcctggt ttagccataa atgttgtttt gttcatgatt
    56341 tctcctatat atagttcgat ttttgtttac ggtgatgggt gttccttccc atcgaaaagt
    56401 atttggaagg ttccggggcc tttcaaatgg ggtaaacaat accgcctact atcataccaa
    56461 atttcacccc taaaagtcaa tagatatgaa aaatattttt cggtaacttc tcaaagtaac
    56521 tgaaagttat ataatcaagt taatttggga atttacctat taacttcggt agatttttgg
    56581 gaattttttt ccttcttttt ccctgtagtt aaaatccttt tcaaaaaatc accttttctt
    56641 gatttttaat cctatttgct gtatgataag ggaaaagaaa ggagacagag atatggcttt
    56701 taccaatacc cacatgcggt ctgctagttt tggcattgtt accagcttgc ctgatgacat
    56761 cattgactct ttttggtata ttatcgacca tttcttaaaa aatgtctttg aattggaaga
    56821 agaacttgag tttcaattgc tcaataacca aggaaaaatt accttccatt tttcaagtca
    56881 acacctccct acagctattg attttgactt taaccatcct tttgaccctc tatatccgcc
    56941 aagagtactg gttttagaca tggatggtag agaaactatc ctcctcccag aagaaaatga
    57001 cctattttaa aaactctagt cttcagttac aaatgactga aaactagagt ttttttattt
    57061 tttcaaagca ttatacaagt tgcggatcgg ttgctttaat gtcggatgga taaaatgaga
    57121 cgcaatttcc tgtaaggact caaggacaaa aaggcgttcc gctatgtaag gatgaggcaa
    57181 gataaggtca tctgtataaa ggatctggtc ctccacaaag agcaagtcca aatcaatcaa
    57241 acgaggtccc caatgcactt ctctcacccg tcccatctct gactcaatgg ctaacaaggt
    57301 ttctaacaag actggtgctg gtagccaggt ttctacttca atcacctgat tagcaaagct
    57361 atcctgctcc actccacccc aaggctccgt cgctaagaca ctggactctt tgagaatatg
    57421 gatgccacga gctcgcagtt tgtcaatggc ttgctccaag tttgcttgtt tatctcccat
    57481 attgcttcct agggcgataa aggctcgttg cttgcggcga tgaatggtta ccgagcaagt
    57541 atctagtggc aaatgcactg gtgcccaagg tttttttagt tccagcttta tttcttggac
    57601 aagaggatag gtctcaaaag tacgttccac cagtttatag gctaccgttt caatcaaatc
    57661 ttcactcgtt tcctgaaacc aagtcgtcca ctgctgacac aattctccgt aatggacaga
    57721 ggctgttaaa tccaagtctg tagctgcctt ggtcatatca taggatagga tggctgaaac
    57781 gacaaatttc tgccccaatt ctttctcact aggaaaaaga ccatgatagg caaacatttc
    57841 caaatcctta atctgcagtt gatccataat gattcctttc tagaaaaatc cgcttgaaac
    57901 ggattctttt tatagcccca ttaaacgata agcttggtcg cggaggtcct tatctgtttc
    57961 aaatagacca cgagctactg tcgtcaaggt tgcagtgcct ggttttctaa caccacgcat
    58021 actcatacac atatgttccg cctcaatgac aacaaaggct cctttagcac ctagatagtc
    58081 catcaaggca tcggccactt cgatattcaa acgttcttga atttgtggtt ttttcgaata
    58141 aacttcaacc gtacgggcta gcttagacaa gcctgccaca cgaccatctg gaatgtaggc
    58201 aatgtgcgct ctaccataaa atggcaagaa gtggtgttca cacatggtat ggaaaaagat
    58261 atccttttct accaccatat tatcgtcaat aatttcaaag gattttgaca aatgttcctc
    58321 tgctgtttga ccaagacctg aaaaaatctc ttgatacata cgggctacac gagcaggtgt
    58381 ttcctgcaag ccctcgcgat tagcgttctc tcctacagcc tcgataatca tttttacagc
    58441 tgcttcaatc ttttgtgtat ccattctcgt tcttcctctc catcagatag gccctcactt
    58501 ggctcaagaa atacaaggaa cctgtgacaa tcctaactgt ttgtttctct tcttttttat
    58561 ctgtcaaatt ctgctctaga aaatcatgcc aatcttggta gctgagattt ctagacttag
    58621 ctgcctcttt cagcacgctt tcatccgtcg cccgactatc cgcaaaatgt gttagagtaa
    58681 gctcggtatc tggcatagct cccagcaagt ccaacatatc ccccaaggct ttggttttga
    58741 tacaagtgaa gaggatttcc ttatgataat ccgcaaaacg ttcttgcaag gttaccaaca
    58801 aggccttgat agcatgggga ttatgggctc catccaaaat catcaaggga tctcttgaca
    58861 cgatttccaa acgccctggc caacttgttt cttccaaggc ttgaccaaga aagtcattgc
    58921 ttgctagctc tcgaccatct tcttgacaaa aagtatcaag taaagctatg gccatcccag
    58981 cattctctat ttggtacaaa ccaagcaggc tagtctggaa gcgaccttgt ctgacagcac
    59041 ttgtatagtc aaagacttcc cctgtcacca cactttcttg atgacgaacc tgataatctg
    59101 tcccgtaggc aagtctcggc gcatctttcc cttccgcaat gcggtcaatc acagccaagg
    59161 cttctggagc gatacgacct gtcaccaagg gagttccttg tttgataatg cctgccttct
    59221 gctctgctat ggcctccaag gtgtcaccaa gtagggccac atgatccagt ccaatagtgg
    59281 tgatgcctgt caaaatgggc tggcaaacat tggtactatc caaaagtcca cccatgccaa
    59341 cttccatgat ggccacatct acttgctcta aggcaaagta gtcataggct atggctgtaa
    59401 ttatttcaaa ctcagttgtc ccctgcaaat tggtagccga ttccccctca agcaaagacc
    59461 ggtagtctgc catgagggct tctagcctag cctctggaat ggattccccg ttgatgctaa
    59521 tctggtctgt gtaatgaatg agatagggag agctaaacac gccaactctc agccctagct
    59581 tttctagcat ctttttcaaa aaagcaatgg tcgaaccctt gccgttagtc cctccgatat
    59641 ggatgacctt gagtttgaga tggggattgc cacgcaaagc taacagttcc accattcgtt
    59701 ccaagccaaa atgcggttga tccgtccggt agttagcaat ccactgattg ttttcaaatt
    59761 ctttcatctt atttatattg ttttaaatct aaattttccg cttcatcagc cagacgaata
    59821 gcggaggcaa tttcaactgc catcctgtga ctagctacat catgcacgcg caccacttcc
    59881 acaccctgtc tcacagcgat actggttaca tgagccgaag ccgtatctcg attgcggaaa
    59941 ccaagttctg tctcaggatt gacttcaaaa ccattttctt caagaatatt gatgacaaac
    60001 cgcttgcgcg acactccgag aaagattgga tagcctttct gatgtagttt atccagatcc
    60061 cgtaaaagaa gcagattttc tttcttggtc aaaccaaagc caattcctgg atccaacaag
    60121 atattttctt gtgcaatacc agcttcctct gctctcgcta aggctctgtc aaagaaagtc
    60181 tccatcaagt cttctattgg caatgtttca aagtcagcta actctttttc tgtaaaagct
    60241 tgaccaaaac caaaatgagg aaagatgagc gagctagggt gctgaggtcg tgctatgact
    60301 ggattaaaca taatgactac tttcgctcca gccttagcaa ccacatgggc cattttttca
    60361 tcacccatga gaccagtgat atcattgact agattggcac cagcagccaa agcagcctct
    60421 gctacctgac tcttccaagt atcaatagag atgaggacat tactttcctt gcgaatagct
    60481 ttgattactg gaacaacacg ctggatttcc tcttctatct caacatagct gcttcttccc
    60541 ggccgagtcg attctccgcc gatatctagc atactagccc cttctgctat caatttacga
    60601 gcctgctgga gtgcttgctc aagagcaaaa aattgaccac cgtccgaaaa ggagtctggg
    60661 gttacattga taattccgca aatagctgtc tttgcatgat tggctttact tgacatatcg
    60721 gtcactccct caaggctttt caccatatta tttctctatt ttaccataaa aagaaaaaga
    60781 tggacacaat tgcattcatc tttttcccag tagaaacaag taagcaattg tcaaaaatct
    60841 taaacagaga tccctaatgt ccgactcaaa atcaccacga gagccaacaa acagaaagca
    60901 accccattca caatcatgtg aagtaagata gacatttcca aacgttgggt cttgtaagcc
    60961 gtccaagata gaacgatcga catacctcca taaatcaata aagaaggtaa attacttggt
    61021 tgatgcaata aagcaaacac aaccgtaccg actacaaatc ccaagttctc ttttcctcgg
    61081 aaaatctttt taggaacaat cccacggcac aagatttcct cacaaattgg agcaagcaag
    61141 gccaacaaga agaaactgga aattaaggaa ctattctgaa ccatatcgtt aatctgagac
    61201 tggttagctg ttgtcgtctc atttgacagt tgcaacaaga tagaaccaag tatatttgac
    61261 ccgacaataa ctaaataact caagcccaaa cgtgccaaat ctttagctct aaaaaaagaa
    61321 aaattaaaac tagctaactt ggttttacga gctcccataa taaatagagc cagaaccaca
    61381 attgaaaggc cagtaactat cagccctgac tgtagcaggg ctacttcttt taaagtcaaa
    61441 atagaggtaa ccactagggg aacctgatat aaaaggacgg caagtaaaaa gaccaaaatc
    61501 cagcctgcac gattcaacaa ttctttccac atatttttct ctttcatcac ctatctcctt
    61561 tgtattaaaa aaagggggaa atccccctgg gtcattctat tataaggcca tgctgatgta
    61621 gttaaggata aacaaggcat ccaaaatcca aatcatggca tgaacatctt taacttgacc
    61681 tttgacaagc ttagtcaaag tgtaagtcaa gaaaccaact gcaatccctt gagtgataga
    61741 gtagctgaat cccataaaga tagatgtgaa gaaggcagga actgcttcag acatatcatc
    61801 ccaatggata tttttcaagc taccaagcat cataatccca acgataatca agattggagc
    61861 tgtagccgct gttggtacga tcgctagaag tgggctaaag aagcttgaaa tcgcaaaaca
    61921 gatagctaca accaaggctg tcaaaccagt acgtccacct gcaccgatac cagcagcaga
    61981 ctcaacataa gtcgttacgt ttgaagtacc tgcaatggca ccgactgtcg ttccaatcaa
    62041 atcagagtaa agagccttat ccaatttggc tgattggtga ttttcaccat ttgtcgctac
    62101 gataccaact ttttcacctg taccgatcaa ggtaccaatt gtgtcaaaaa tatctgtcaa
    62161 tgagaaggca agaatggcca tcagagtttc aggcaagcga gctgtatctg aaaccaaagc
    62221 tcccaaacct tctgaaccaa gagctgcacc aaagattgtc ttcaaatctt caaaagctgc
    62281 accaacatgg ttattagcaa aatcgatact agacaaatca accaaaccaa ctgcaatagc
    62341 aagaacagtt gttgtcaaga tagagagaat aattccccct ttaatccctt tgatgacaaa
    62401 gaagatagta atggcaagtc ctgcaagagc cactaaaaca gctggattat taaagctgac
    62461 caatcctgga actgctgaag agtttgctgc aatcgttgct tgagctttgt cagccccttc
    62521 tcctacaaca gtatagttgc ctggatcaat cgtgaatttc aaaagtccag cattcttaat
    62581 ccctacatag gcaaggaaga caccgatacc agctgaaata gctgagcgaa gagcattggg
    62641 aatcgattca atgatcattt tacgaacatt tgtcaaggta ataatcaatg aaataatccc
    62701 acagatgaag accatagcta gggcttcttg ccaagaataa ccaagcccga atacaactgt
    62761 aaaggtaaag aaggcattga gtcccatacc tggcgcttgg gcataaggta agttagcata
    62821 aaaagccatc atcaaggtac ccgctactgc accaataatc gtcgctagga agacgccctg
    62881 agcaggcatt cctgtttgtg aaagtatttg tgggtttaca aagagaatat agctcattgc
    62941 aaagaaagtt gttaaaccag cgagaacctc tgtacgaacg tctgtaccgt tctcttttag
    63001 tttaaataat ttgtccatca tcaatctcct tttaattttt acgaacaata atagaattat
    63061 atcatttatc attcactttt tcaatgtctt tgtttgattt gtttaagaaa ttaatccaat
    63121 ttcatttttt gtttggaatc tgcttttctg cctgctttct gttataatag aagacatctt
    63181 atctgaaaac acactagaaa ggtccatatg acgaaaaaaa ttattgcagt tgacctggat
    63241 ggaaccctgc tcaactcaga cagtcaaatt tctgacttta ccaaaaaaac catcaaaaaa
    63301 gttgctgaaa aaggccatca ggttattatt acgacaggtc gcccttaccg tatgtcaaaa
    63361 gatttttacc gtgaactggg cttagacact cctatgatta acttcaacgg atcccttact
    63421 catttaccag accaagtttg ggattttgaa aagtgtttga ctgtagacaa aaaatatctg
    63481 ctagatatgg ttcaacgttc agaggacatt caagccgatt ttatcgctgg agaatatcgt
    63541 aaaaaattct acattacaaa tcccaatgaa gaaattgcca atcccaaact atttggtgta
    63601 gaagctttcc agcctgaaga tcaattccag cctgaattgg tgaccaagga ccctaactgt
    63661 atcctcttgc agaccagagc cagtgacaaa tatgccttgg caaaagacat gaacgccttc
    63721 taccagcatc aactttctat caatacctgg ggaggtccgc tcaatatcct tgaatgtacc
    63781 ccaaaaggtg tcaacaaggc ctttgctttg gactacttgc tcaagataat gaatcgtgac
    63841 aaaaaagatt tgattgcctt tggagatgaa cacaatgata ccgaaatgct cgcttttgct
    63901 gggaagggtt atgccatgaa aaatgccaat ccagagctac tcccttatgc agatgagcaa
    63961 atttccctta ccaacgacca agatggggtt gccaaaaccc tacaagactt attcttataa
    64021 cctatactga tactcaatga gaatcaaaga gcaaactagg aagctagccg taggttgctc
    64081 aaagcactgc tttgaggttg tagatgaagc tgacgtggtt tgaagagatt ttcgaagagt
    64141 atgaccagct tgcaagctgg tctttgtgct cttttcacag tctcatcaac cagttaatcg
    64201 attgttctac tttttgtctc caaaaccttg gaaaaggctt ccttttgtga tatacttcac
    64261 atataaatac aagagagctc gtcacactcg actctgttga caaaaaatca atccagtccc
    64321 gttgtccctg ttctcggtca atatcaagga ggatagctat gaaagctgtt gttgtaaatc
    64381 cagaaagcac tggtgttgct attgaagaaa aagtactccg tccacttgaa actggggaag
    64441 cacttgtaga agttgaatac tgtggcgttt gccacaccga cctccacgtt gctcatggtg
    64501 actttggtca agtcccagga cgtgttcttg ggcacgaagg tatcggtatc gttaaagaga
    64561 ttgctccaga tgtgaaaagc cttaaagtcg gtgaccgcgt cagcgttgct tggttctttg
    64621 aaggatgtgg cacttgcgaa tactgtacaa ctggtcgcga aaccctttgc cgtacagtga
    64681 aaaatgctgg ctactcagta gacggtggta tggctgaaca atgtatcgca actgctgact
    64741 atgctgtcaa agttcctgac ggacttgatc cagcccaagc ttcttctatc acatgtgctg
    64801 gagtaacaac ctataaagct atcaaagaag caaaagttga accaggccaa tgggttgttc
    64861 tttacggtgc tggtggactt ggtaacctcg ctgttcaata cgctaaaaaa gtattcaatg
    64921 ctcatgttat cgcagtcgat atcaacaatg acaaacttac ccttgcaaaa gaagtaggcg
    64981 ctgacattgt gattaacggc ctcgaagttg aagatgtagc tggactcatt aaagaaaaaa
    65041 ctgatggagg agctcattca gctgtcgtaa ctgctgtgtc taaagttgcc ttcaaccagg
    65101 ctgttgactc cattcgtgct ggtggtcgcg tcgtcgctgt tggtcttcct tctgaaatga
    65161 tggaactcag catcgttaaa acagtcctcg atggaatcca agtcatcggt tctcttgtcg
    65221 gaactcgtaa agacttagaa gaagccttcc aatttggtgc agaaggtctg gtagtcccag
    65281 ttgttcaaaa acgtccagta gaagatgctg ttgccatttt cgacgaaatg gaaaaaggcc
    65341 aaatccaagg acgtatggta ctcgacttca cccactaatt taatagaaac ctattttaaa
    65401 agttggaagc ttcttccaac ttttttat
//
LOCUS       DAVKDZ010000002        64548 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000002 DAVKDZ010000000
VERSION     DAVKDZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 64548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64548
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.002"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            118..309
                     /locus_tag="VWZ30_000062"
     CDS             118..309
                     /locus_tag="VWZ30_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9333888.1"
                     /translation="MPDITNGREKVNDFLKDKGIKKTSLAIAYGFKRQEVTNILSGTT
                     KGPRANSFILQVIEDYGIE"
     gene            321..1028
                     /locus_tag="VWZ30_000063"
     CDS             321..1028
                     /locus_tag="VWZ30_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HEU9333889.1"
                     /translation="MNEVIKVTVNDNHEPIVSGRQLHEALGVKTEYKKWFSRMTEYGF
                     NENEDFLKVTQKCLTSSTGQNTTDHIIKLDMAKEIAMIQRTERGKQVRQYFIQVEKDF
                     NSPEKIMARALLMADQKVHKLEAQIEADKPKVLFADAVSASHTSILVGELAKLISQNG
                     YKIGANRLFSWMRENGYLIKRKGSDWNMPTQRSMDLKLFEIKETNVQHADGHITVNKT
                     PKVTGKGQQYFIDKFLN"
     gene            1214..1555
                     /locus_tag="VWZ30_000064"
     CDS             1214..1555
                     /locus_tag="VWZ30_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEU9333890.1"
                     /translation="MMVVLANHPNWQVYPDEIAKRKGVNRKTIDKYFKIFEEAGYLRK
                     IRKKPPGNGGSHIFRFFSDVKISDFQFDIMKQRLNLSIKRASMNYNSDIPKSEMSESE
                     MSESEMSDFGH"
     gene            1712..1978
                     /locus_tag="VWZ30_000065"
     CDS             1712..1978
                     /locus_tag="VWZ30_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333891.1"
                     /translation="METVQIVRIKDVIIEKISANDEELERIFGCSKRQAGDMRREMKK
                     LPSQQKYLRNDGQLVTIKGFDAYLQYRGSQSWKKEMAKTVKMTR"
     gene            2062..2514
                     /locus_tag="VWZ30_000066"
     CDS             2062..2514
                     /locus_tag="VWZ30_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333892.1"
                     /translation="MPNWAEGTLKLRGRRENVASALKEMLLGDEGATLEEEYDGTLLR
                     FKNEYDYFYINGTRRAFISSKDIEIWLDDDFVIIELEDFEQAWAALADNYTEISSKFD
                     VDIKIFTFEMGMEFTQEIEISKGEIIKNIVNENFTNYSWDVPFSRLGG"
     gene            2520..3221
                     /locus_tag="VWZ30_000067"
     CDS             2520..3221
                     /locus_tag="VWZ30_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HEU9333893.1"
                     /translation="MADLTFAELQRKMQIEKQTKQGVKYPFRTAEDINNKFKSLDSGW
                     SVSFPEDDIIQKGDKLYYKAVAVAKRESDGTIEKAIGWAREEDVPIFHTQKGDVKQMQ
                     DPQWTGAVGSYARKYALQGLFAIGGEDVDEYPVEESQEQGQNNQQQKPNNQQAQGQNQ
                     VRYIDNIQYQEINDLINDIAKIKGMPFDTLANYVLSEKLKGLQDFHRVQVGDYEVLKN
                     YLTEQLAKAKAKRGN"
     gene            3224..4216
                     /locus_tag="VWZ30_000068"
     CDS             3224..4216
                     /locus_tag="VWZ30_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HEU9333894.1"
                     /translation="MVKDVTNSLTEIKVDFQPAVINVDREAIEAQVAAAIAQYSGREV
                     TVDNYKEVYEERTRFNKLIGGLDTQRKDFNRQINEPAKDFDKWVKEKVIKPIEGVTDA
                     MSAGLNAIDEHERLMRVDVVRATFEDKCMVAGIEKSTFADKYDEYSLKKYFKTGKYEL
                     KKTTLDEMDGLVLSEFDALEEYKANKQAIQEQAQEYDLPADSYIRHLEDGKSLVDILK
                     MMKTDRDAEIARKEQKEAQEKAKAERLEEIAQSAKKNANANIKAYDAETGEILEQGTI
                     TPEPQNNAREVAKFEPSEPLVKLVRLELHGGLEQWENTQEYFEDNFIGFETLED"
     gene            4238..4414
                     /locus_tag="VWZ30_000069"
     CDS             4238..4414
                     /locus_tag="VWZ30_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333895.1"
                     /translation="MRCFYVSGKIADLDLGSEINAENSFMAAIEFVKRYTGLLKFGSN
                     EIKVSEVEEVQNDK"
     gene            4404..4949
                     /gene="ssb"
                     /locus_tag="VWZ30_000070"
     CDS             4404..4949
                     /gene="ssb"
                     /locus_tag="VWZ30_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU9333896.1"
                     /translation="MINNVVLVGRLTRDAELRYTQSNIAVATFTLAVNRPFKNEAGER
                     EADFINCVIWRQSAENLVNWAKKGSLIGVTGVIQTRSYDNQQGQRVYVTEVVASNFQL
                     LESRNSQQNNQGHQDHHGGYQQQGYSNQGSSFQNGNSYGQQGSFLEGNTTNLVPDFTR
                     DNNPFGRPTNPLDISDDDLPF"
     gene            4975..6108
                     /locus_tag="VWZ30_000071"
     CDS             4975..6108
                     /locus_tag="VWZ30_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU9333897.1"
                     /translation="MKFLDLFAGIGGFRIGMESAGHECIGFCEIDKFARASYKAIHDT
                     KGEIELHDITTVSDDTIRGIGHVDVICGGFPCQAFSIAGNRRGFEDTRGTLFFEIARF
                     ASILKPRYLFLENVKGLLNHDRGNTFEVILSALDELGYDVEWQVLNSKDFGVPQNRER
                     VFIIGHSRKRGTRRVFPIGRANREFDFEPKIKIVGNTKNPNGTSQGTGSVVYDSNGLV
                     GTLCARDYKEPKQVAIPVLTPDRVNKRQNGRRLKKNGEPMFTLTAQDRHGILIKEATK
                     KGYAEATVGDSVNLSHPNSKTRRGRVGNQIANTLLTGESQGVVEPDFRIRKLTPKECW
                     RLQGFPDWAFDKAQEVNSNSQLYKQVGNSVTVNVISAIAQGLG"
     gene            6152..6355
                     /locus_tag="VWZ30_000072"
     CDS             6152..6355
                     /locus_tag="VWZ30_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333898.1"
                     /translation="MTKIEIVMVLTTLMSITWAAIVTIHAMQAIKKHKAKVDYYQKPQ
                     VQCEIARHVLKNKWYSDGGEVFR"
     gene            6409..6843
                     /locus_tag="VWZ30_000073"
     CDS             6409..6843
                     /locus_tag="VWZ30_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333899.1"
                     /translation="MDKKLIGLDLTHIADGGLQEKLDKELEKVFDNILDLNTDAKAKR
                     KVTITLTMSANEERTVVDTTMEVKSKFAPQNGVATTILIGRDFDTGQVHANELKSTVP
                     GQMYFDENGEILTDIGQPVAEIEQQAETKPDIIDFNKKKVGN"
     gene            6845..7582
                     /locus_tag="VWZ30_000074"
     CDS             6845..7582
                     /locus_tag="VWZ30_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333900.1"
                     /translation="MTTENLKAALEYAVELNEHGLEILTAADGTEYYDANKFNLKELD
                     PKRYPKTLELSTLTSLVDYLKTDLNNLKNQRLIVAVEKNDEVCVWSENDEIEHRTLLV
                     DVKARIPELSFGRFLSLEQFNIMLQSNFIDDNDRGTLLEFASALKIENGAEIEDNGVS
                     QVATVKTGVASLAKGKAPNPVTLRPYRTFSEVEQPASLFVFRIDKQANMALFEADGKR
                     WVADAVGNIASYLKEQLADQKHITVLA"
     gene            7599..7811
                     /locus_tag="VWZ30_000075"
     CDS             7599..7811
                     /locus_tag="VWZ30_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333901.1"
                     /translation="MSDYKQRMIEEYKQLKERTNKLSLMISNYYVGTLDFKLKCPIEL
                     LETQHYTMCAYLKILEQRAEIENIEF"
     gene            7822..8223
                     /locus_tag="VWZ30_000076"
     CDS             7822..8223
                     /locus_tag="VWZ30_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333902.1"
                     /translation="MKFEFSLPRNTKLKSLNMVINSNDRQHQTDKAKVTKRIRAFAYW
                     HTSMNKDKERAAFSPSNPCEVTVTIYSPTKSKLDPPNLYPTVKAIIDGMTDAGIWTDD
                     NHKVIKKLSFVYGGLSEEKGHYRLEFDIEEV"
     gene            8225..8431
                     /locus_tag="VWZ30_000077"
     CDS             8225..8431
                     /locus_tag="VWZ30_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333903.1"
                     /translation="MIEVNIKFDNFEAHGFYQDDTKLGKIRDAIISQMNNGHVVVLGE
                     DRGILLNPKVIKSVQFKVVEDNQI"
     gene            8480..8797
                     /locus_tag="VWZ30_000078"
     CDS             8480..8797
                     /locus_tag="VWZ30_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333904.1"
                     /translation="MMEELKQKVNAVYNWTVEDGKPKPPKQDLPQAVKDRVDYFWEMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKGWLPKSKEFDDWIGYAPSMAQVVIAVYLIY
                     GGN"
     gene            8799..9299
                     /locus_tag="VWZ30_000079"
     CDS             8799..9299
                     /locus_tag="VWZ30_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEU9333905.1"
                     /translation="MNIKALIKKYEELWNEHSPFYEPVPYTSMVELFLKELKQLDEPE
                     KVKVPQFMADWIDYFKKNGDWDLFQAMDYLFGKKEIREWLEYKNNQDLFARAWLDGYE
                     VEKEKRYLVTLKNRQPLVKSQSGSTLYFSQDITARNYKGTQKELEDAKFGWVFDCPGI
                     EIEEVE"
     gene            9299..9592
                     /locus_tag="VWZ30_000080"
     CDS             9299..9592
                     /locus_tag="VWZ30_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333906.1"
                     /translation="MTQTLEEGMKNQSKCIKIPMEIRPFDVGYRIVNKHGQALALKNG
                     ASIFDLPSLAEKAIKKEFSKDDPDFDIEKHFVEEVAIVNLSKFHSYFEEVIHD"
     gene            9592..9984
                     /locus_tag="VWZ30_000081"
     CDS             9592..9984
                     /locus_tag="VWZ30_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333907.1"
                     /translation="MTKKKIERLSVIHRREINWLKWYFLRDKKNPKRTILEQKIIVSH
                     IKTDRLEAKFLSNLKKSTEDFIDKSDPKYLRAIKEVYVYENMNVIGACQKILFYSPTQ
                     AYVLLNAWFNDYFRATYTELLENAILDK"
     gene            10050..11144
                     /locus_tag="VWZ30_000082"
     CDS             10050..11144
                     /locus_tag="VWZ30_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4417 domain-containing protein"
                     /protein_id="HEU9333908.1"
                     /translation="MEMLQIEYVDIKSIKPYHKNARHNDGEATEKVAASIKAFGFQQP
                     ILVDDNNIIITGHTRLKAALSLGIDTIPIAHAVNLTDEQIKAYRLADNRVAEYSTWDS
                     ELLNIELSQFETIDMAQFGFELSVTGFNFGNEEEQQEETENEEEDAEDFHRDTTINQY
                     NLFHYDDTRVEGFYNMPKIEGVDHIPKDFQGFNYVLNKPDYSSCVHFFLDDYQFERIW
                     QRPDFYIEKLLEFDSALAPDFSLYLDMPIAMQVWNIYRSRLIGQIMQDYGLTVIPTVS
                     WASEESFDFCFDGLPKNSTLAISTIGVKQNKEQFEVWKNGVTEMIKRLTPKRIVVYGG
                     KVEYDYKDIEVVYFENATTERMKESGTKTN"
     gene            11125..11871
                     /locus_tag="VWZ30_000083"
     CDS             11125..11871
                     /locus_tag="VWZ30_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333909.1"
                     /translation="MAQKLTKLKDIFKHVSSIDLGKEILFEDLELYNKETETSKQYQS
                     IEEAENDLSLMEKVNKINFTLGGGRGANFEKGKDGKYPGFRGAGGARDSGSSKALHPA
                     SLNNQGRFSSVEGTIQEFIKKHGGSRTEYSTAVDSQGFAHNYVHGGKNSVQILPISGG
                     FTAIHNHPNGSNFSSTDLHSFAALKGMNTLVATNSSKAYRITKGANFDAKGFDKAVSK
                     SRFTTKDYNKGADLWLKKNAKKYGYTYSYE"
     gene            12075..12530
                     /locus_tag="VWZ30_000084"
     CDS             12075..12530
                     /locus_tag="VWZ30_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein"
                     /protein_id="HEU9333910.1"
                     /translation="MPRDGTKNLKPVTERTKDEARAISSKGGKASGIARRKKADLKKA
                     FEILLSLDVTDSKIKKQLEEMGMAGNNEALLAFATFQQAVKGNQKATENIIKLTNTKD
                     KYDIQEQKERIKALKYENRERAEAEKGSSETIEIVDAWAEDVRGATDDL"
     gene            12520..13830
                     /locus_tag="VWZ30_000085"
     CDS             12520..13830
                     /locus_tag="VWZ30_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEU9333911.1"
                     /translation="MTFNVQKNINPHFKSVWISSLPYNVLKGGRNSFKSSVIVLKLAY
                     MMIRYIIAGEAANIVVIRKVANTIRDSVFNKVWWALNLFGIAEQFTKTVSPFKIVHKT
                     TGSTFYFYGQDDFQKLKSNDIGNIIAVWYEEAAEFNDQEDFDQSNVTFMRQKHPRAKF
                     VQFFWSYNPPRNPYSWINEWFESIKTNKNYLAHSSTYLDDELGFVTEQMLEDIERIKE
                     NDYDYYRYLYLGEAVGLGNNVYNMSMFHAIDALPSDDKLIGISFALDGGHQQSATACC
                     AFGITAKGKVILLDTWYYSPAGQVVKKAPSQLSKEIYAYMRSVIEKYRVQALQYTIDS
                     AEGALRNQMFLDFGLKWHPVAKLRKVTMIDSFQSLLAQGRFYYLNTENNKIFIEEHKM
                     YRWDEKTIKSDNPSVIKEDDHTCDTTQYFVLDNAKLLGLRVGNV"
     gene            13843..15411
                     /locus_tag="VWZ30_000086"
     CDS             13843..15411
                     /locus_tag="VWZ30_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEU9333912.1"
                     /translation="MSLFQKVKDFFSRGRYYMQTSNLNSILEHPKIAVTQEEYDRIKR
                     NLVYYQSKWDDVQYKNTDGDIKSRPMNHLPIARTASKKIASLVYNEQATITTKNEILQ
                     KFLDDMLTNDRFNKNFERYLESCLALGGLAMRPYIDGDKVRVAFIQAPVFFPLESNTQ
                     DVSSAAILTKTIKSEGRKNVYYTLVEFHEWVTADGQETGSTNDKKYYRITNELYRSDV
                     NDVLGQRVNLSELDKYKNLEPVTVFENLSRPLFTYLKTPGMNNKDINSPLGLSIFDNA
                     KTTIDFINRSYDEFMWEVRMGQRRVIVPEHLTQRQYQRPDGTIDFRPRFDVEQNVYMQ
                     IGGSSMDAGGITDLTSPIRANDYILAISEGLKLFEMQIGVSSGMFTFDGQGMKTATEI
                     VSENSDTYQMRSSIVALVEQSIKELCVSMCELGKAVGVYSGEIPELDDISVNLDDGVF
                     TDRHAELDYWAKMVAAGFSTKKRAIGKTLNISDVEAEKELNAINSELLPMNDAELAIY
                     GMHDQNEEEADDKG"
     gene            15398..15571
                     /locus_tag="VWZ30_000087"
     CDS             15398..15571
                     /locus_tag="VWZ30_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333913.1"
                     /translation="MTKVKFGVTSVDYSASIEDTPTIKLGLIIRGSGRLDASSVIKKL
                     IKDVSELEYELEE"
     gene            15614..16765
                     /locus_tag="VWZ30_000088"
     CDS             15614..16765
                     /locus_tag="VWZ30_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor capsid protein"
                     /protein_id="HEU9333914.1"
                     /translation="MKKKRKQLTFNDQQFPLQMQGVGDIYEKLQIDIFDRMIKRLKER
                     GSIDLMRNPYIWQLEKLNDMHMLNEQNLKLISERTGIAERLLRDVIENEGLKVYKDTK
                     QQLEEDLNKIPEGEISNGVTDSLEAYSRQAVSDLNLINTTLPKSLQVAYKSIVEETVA
                     QVVAGTKTSDVALHDTIMKWQKNAFTGFVDKGGRHWKADSYARAIIKSTTYKVYNEMR
                     TRPAEELGVDTFYYSMKAMARPACSPLQGQIVTKGAGREIDGITIYSLLDYGYGTAAG
                     CLGIHCGHYLTPFIVGVHELPNLPDYLKNLTPEQAEENARIEAGQRGLERLIKTHKER
                     LHYAHTLQDDKMIQAERLKVRGYQTKIRNLINQHDFLTRDYRREKLYIS"
     gene            16904..17467
                     /locus_tag="VWZ30_000089"
     CDS             16904..17467
                     /locus_tag="VWZ30_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HEU9333915.1"
                     /translation="MAFTTEELLNLGLTEEQAKSVFALRGKELNEDKSALETIKQERD
                     SLKSQLQKAEEQVEHLKSLENISAEQKDAIDKLQAEYDKYKNEAAAELAQTKKVSAIS
                     LALKDTNAFNPDKLMKFIDVDAIQLDDNGKPQIDEVINGLKESDPYLFKAEESKPSPN
                     ILPQGNPAGEGTSDVDPFQAIIDGYGK"
     gene            17485..18366
                     /locus_tag="VWZ30_000090"
     CDS             17485..18366
                     /locus_tag="VWZ30_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001139822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEU9333916.1"
                     /translation="MPSNQNNAVRRYEKQYAGILETVFGVRAAFSNALAPIQILDGVQ
                     ENSTAFSVKTNNTPVVIGEYKTGENDGDFGDNSGAQSRFGGVTEVKYENTDVNYDYTL
                     TIHEGLDRYTVNNDLNAAVADRLKLQSEAQTRTVNKRIGKYLSDNATKTEALADFTDD
                     KVKALFNKLSAFYTNNEVTAPITVYLRSEFYNAIVDMASVTSAKGATISLDENGLPKY
                     KGFTLEETPAQYFETGVIAIFSPNGIVIPFVGISTARVIEAENFDGVKLQAAAKGGTY
                     TLDDNKKAIYKVTGTIV"
     gene            18377..18610
                     /locus_tag="VWZ30_000091"
     CDS             18377..18610
                     /locus_tag="VWZ30_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333917.1"
                     /translation="MALYKATKNLFFEQLNMDVIVDDIIKLDEDYAKEVNKKLKNAFP
                     DVKNVLELVDKNGTLEPEDAPSVDDASQATVED"
     gene            18653..19045
                     /locus_tag="VWZ30_000092"
     CDS             18653..19045
                     /locus_tag="VWZ30_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013794407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333918.1"
                     /translation="MTYLTQEEFDELGFDEVTDFEKLAKRAKIAIELYTNGIYQKDID
                     FEKEIAYRKSAVKLAMAFQIAYLDASGIMSADDKQLANSVSIGRTSISYSTSQSTLAG
                     QRFNLSMDAENALRQAGFSLVVGVAYDR"
     gene            19035..19427
                     /locus_tag="VWZ30_000093"
     CDS             19035..19427
                     /locus_tag="VWZ30_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HEU9333919.1"
                     /translation="MIDKRLLKGIDKRLLKDVLTIKKVADKNDYGDEVYSEPLTIKNV
                     RFDRSVGGSGNRNSKTGTGNSKTGTGNSKSRQKQGVIYLYPSLSFVTVDNSWMGAKVN
                     DGIGDYTINGFQTNYYDGEIFSQEIEVI"
     gene            19427..19771
                     /locus_tag="VWZ30_000094"
     CDS             19427..19771
                     /locus_tag="VWZ30_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HEU9333920.1"
                     /translation="MSIAIKVDLQKAKQKLSNESMTRGKIAVASKILLNNEQYIPLRG
                     GELRASGRIVGQGDAVVYGTVYARAQFYGANGIVTFRRYTTPGTGKRWDQVATSKHAE
                     EWARAFVKGMGL"
     gene            19771..20178
                     /locus_tag="VWZ30_000095"
     CDS             19771..20178
                     /locus_tag="VWZ30_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015968622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HEU9333921.1"
                     /translation="MRENDFQNVLLKHIKTLNLPVEPRFDYFEDDKDDLVINQIPGGK
                     VDREYMDGTQEVSLPFEIAVKAKKNSVANDTIWLVTSELAKIDLVLPSDNNSYEYMGM
                     EVSRPAMKGKDEQGYYYYTIEIVAKIVIERNKQ"
     gene            20175..20624
                     /locus_tag="VWZ30_000096"
     CDS             20175..20624
                     /locus_tag="VWZ30_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU9333922.1"
                     /translation="MTRQKNALRGHFVAPYNGGTEPSTEDTWLELAKWISDVSDDTDE
                     KTDDQAYYDGDGVEETTVVSVKGAYTFEGTYDPDDKAQALIAGMKYKTGDDRKLWHKV
                     VSSDRKKQWVGAATATEIKAGSGAASDYEAFGCKLSYNSTPKETGIG"
     gene            20689..21177
                     /locus_tag="VWZ30_000097"
     CDS             20689..21177
                     /locus_tag="VWZ30_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333923.1"
                     /translation="MTDIQIELKRTGFPVKIGEVELWFDTSQESLMRFYDMEEELKRR
                     LVQYELDVVSANINNKIERDGVTKEVVAGAIELEKKQLEIQYDLIFGDGTFDKLYSIY
                     PDYNALNNALEQTAIMLHDKLEEVAEQHKTVVKERASHYLNKGKVTPIKNNKKQKKNK
                     KK"
     gene            21190..21759
                     /locus_tag="VWZ30_000098"
     CDS             21190..21759
                     /locus_tag="VWZ30_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333924.1"
                     /translation="MSMKLNDALITNFSIADKEYDIDLSFNKVLDVFEILKEDEMTRL
                     EQAQLIVHLLTGQELYDIKEVVDCWIYIKEHFLGIEKETVQYDLLGNPMPKAKGEEEQ
                     EKLIDFEQDAEYIYASFLQAYGINLLKVQNELTWTEFKALLNALPDNTIMQQIIEIRA
                     WKPEYGGDKNKMRKLQAKYSLGKEGEDNV"
     gene            21752..25033
                     /locus_tag="VWZ30_000099"
     CDS             21752..25033
                     /locus_tag="VWZ30_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEU9333925.1"
                     /translation="MSDGKVTIVVDVDGNKVKVLNDELDKTAQKGDRGSSSLKKFAVG
                     SAVFQLAAKGAELLGEALGGAIQRFDTLESYPRVMQAMGHSTEDVTRSTKKLAAGIEG
                     LPTTLNEVVGTAQRLTSITGDINKSTDLTLALNNAFLASGSSSADASRGLQQFSQMLS
                     AGKVDMQSWKTLQETMPYALQKTAESFGFAGQSAQNDFYSALKEGRITFNQFSSKLVE
                     LNGGVGGFAELAKTNSKGIQTSFGNLKNAVVKGVANTIKALDDLTKAATGKTIAENFD
                     ALKVIINAAFGVIVNVIKASTPVFQTLFSILGTGASVISFLTPAIIGLVAALVTMRAI
                     NQAVKTTKDLISAWKTFKTTATGAIQIINLMTAAQATCGSVTKAQLVANLANNGALTA
                     SNLLYGVLTGSISLQTAATIAATAATTAFKAALTALTGPIGLVVAGIGLVVGVCVTLW
                     QWLTAESEETKRLKSEQEELVKSTDQLTDSVKQSAKERQKNLESVKGNTESYQKLADE
                     IVQLSQKTNKTAADKKNLKKKIDALNASVSGLNLAYDKNSDSLSHNSDQIKARISAME
                     AESTWETSQKNLLDIEQKRAEIGEQLKKIAEQRKKWNEESNVSDSVRKERLQELNDKE
                     TELKNTQTELQTEYEKTSQVQQAASEAMAAAAENGSNRQVVAYENMSKSQQKAIDDMR
                     TKYNELLETTTNMFEQIKYKSAISVDEMIANLQKNQEAVNNWATNLNTLAERGVNEGI
                     LAKLQAMGPQGGLYVQELVNASDEKLATLNEVFTQGGESAMNGLTAGMDTGALGITDK
                     IKGIVQSQVSSLQEEIAAADFSSLGQEIPNGVSQGIEQGVSTAGESSKNMANDIKESF
                     TSEMDINSPSRVFNEYGGFITTGLAEGVDKGTNQPVSSVTNLANQIKKPFDSLQSDFT
                     YIGEMAMSGLNAGLWSGSGSVMATANSIAERVKATIKSALDIHSPSRAMRDEVGRFIP
                     QGIAVGIEADAGVVEKSMLRLKESMMIDTRPEIALGLNKKLGAQVTVKQSSKQTIAEK
                     IKVTMDKSSELLEKALDVAETAVRRPNEMYLNDGTLVARTGDKFAKYQSEQLRRDNRM
                     KGVLS"
     gene            25030..26544
                     /locus_tag="VWZ30_000100"
     CDS             25030..26544
                     /locus_tag="VWZ30_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015968624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEU9333926.1"
                     /translation="MTKIMTFNGVDMSKFFRITDIIRPIGNKRSVSTDNAPLLGVNIQ
                     QVKIGEKEHIIKFDIKTTNAIEMEQLKHDLAGILNVLEPVKITYGDEPDKYYMGLPVD
                     EITPENLTRWFQRSELKIIIPDGVAHSTTLKNFDIDTNETSAPDRIVFNLTNTGTEPA
                     YPIIRIKHNSENGYIGVVNNRAAFELGNREEADTEKYRDSETLIDYRGTNILKGFQNG
                     TKGVAVTNDNKERLVGTLSTTSMWGRNHIELSNRGTVEKNRNNAQSLTWAIPVDSSGE
                     VGSLNDYLLWRQVFMAAVANQYGFIKVTVSDTDGNFLYGVETYKRYQTLDCEYSFFTT
                     DGKGGYKFIKWWYFTGTGAQVGKLDPFSAEKGWSELKRNDDRVQVFFDGSHYDFIIPE
                     IKDKKSAKIHITLGALRDWPLISHMYVDEFMYRKDFVTKSRDIPNRYPIGSNVVINSE
                     DDSVYIDGISKVSEVVDGSHWPAIPPGKSQLELYFSRFVKKKPTVTIEFEERWI"
     gene            26546..30631
                     /locus_tag="VWZ30_000101"
     CDS             26546..30631
                     /locus_tag="VWZ30_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU9333927.1"
                     /translation="MLLTIHDANLQKVAFIDNEKQGTLNYYDDTWTRSLATGSSTFEF
                     TVFKKAVKSDLPLAKAYHHLNEHAFVSFKYKGKSFVFNIIIVEENEQTIKCYCENLNL
                     ELINELANPYKSNKAMTFKEYCEAMDLLNYTHLSIGINEISDYKRTLEWEGQETKLAR
                     LLSLAKRFDAEIEFDTQLNADSTIKKFSVNVYHENDDNHQGVGRVRNDVIVKYGKNIH
                     SITRKVDKTGIFNTIRPTGKMPTVEEEPSGDKGSKSETVKNADGSTTKTTISTASDGT
                     KSKTIVHTKVTKLADKTRITTTTTTRSDGSIEQTVTTSKKGGASTSETKVLKKPNPKE
                     KTNTTEDVLTIEGLDEWEVKNEKGIVEFYQRGQALYAPISMQLYPSTFTHSTGELDQW
                     TRKDFHFETDEPNELRRLGYLKLKKYCYPAITYEVDGFVDADIGDTVKVHDDGFAPLL
                     MIQARVTDQKISFTNPVRNKTIFDNFKALENKLSADIQSAFERLFEAAKPYTIKLSTD
                     NGVIFKNQIGQSLVTPTLYKGGKPVVVGVTWRWALDGEVTTGMTYLVRGSNVTDTVTL
                     TVAAYIGNKEVAVDEISLVNVADGKLGTPGTPGRDGRTPYVHTAWANNATGTDGFSLD
                     SSINKLYIGIYTDFEPNDSTDPKKYKWAKVKGDKGEKGDKGEPGQRGLDGLQGARGEQ
                     GLPGRNGADGRTQYTHIAYSNSADGTKDFSVSASDRAYIGMYVDFNSADSNTPSDYNW
                     TLVKGADGANGVAGKAGTDGRTPYLHIAYATSNNGSQGFSTTDSTNKTYIGTYTDYTQ
                     ADSTDYRVYKWTLIKGADGTGISNVTNYYLATTVSTGITRTSAGWTTTPQPITSDKRY
                     LWNYRVELYTNGTSKTTEPTVIGVHGDKGERGLQGLQGLQGARGEQGIPGPRGADGRT
                     QYTHMAYADNATGGGFSQTNTDKAFVGVYIDFNPTDSRNPADYRWTRWKGRDGANGVA
                     GKAGADGRTPYVHFAYSENADGSGLTMTDNGQRYFGHYSDYEKPDSSDKTKYKWADRW
                     AKVDGGYVNIYALSKNRSIGKSYHVSEFNMDVLSGNITLKAIGSDPYIGAVSSHPGIF
                     IKQQGMKIPVIQGRSICITITNPLFRKNYISFFNSLGKTVKTYKHYNTNKFLISSADL
                     VGVEFIALRYGAGSSNIQIGTVLETKVKVEYGTVHSDWSPAPEDIESNINSKADQGLT
                     QEQLNALNEKSQILEAEMKAKASMEAFSELEKAYNAFVKLNADSQKKSESDLVEAGRR
                     IDLLTTQFGGLAELKTFIDTYMKSTNEGLIIGKNDASSTIKVSSDRISMFSAGKEVMY
                     ISQGVINIDNGIFTASIQIGRFRTEQYHLNKDVNVIRYIGG"
     gene            30643..32721
                     /locus_tag="VWZ30_000102"
     CDS             30643..32721
                     /locus_tag="VWZ30_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="structural protein"
                     /protein_id="HEU9333928.1"
                     /translation="MTKFINSSGSLHLNIYIEQVSQDIANNSSRVSWKATVDRDGAYR
                     TYTYGNISNLSVWLNGSSVHSSHPNFDTSGQEFTLASGEVTIPHSGDGTKTFAVWASF
                     DPNNGVHGNITVSANYTLSSIPRSSSVSDNALSGNRRLGSPHTLTIDRKSSLFTHQVW
                     YRVFGSNWIDLGKNHATGVSFVPNIDLARYNTKAKSGTMDICVRTYNGTTQVGNDVYS
                     NGWYFEIPESVKPTFSGITLTDMNTVARQLLSGNNFLQIISDIQVNFNNPSGAYGSTI
                     TGYRAEIVNKNQVTTVNGGRLGMMNFNGSATIRASVVDSRGRQSDTRDITINVIEYFA
                     PAFSFTAFRTRETPNIIQVVRNAKIAPITLSGSQKNVMTLSFKVARLGSTTFTADHGR
                     ASGIWTTQHTLNNSAANMAGNYVATKSFVVIGTLSDKFTSTEFTATVATESVVMSYDK
                     DGRVGIGKVAEQGGAGSLDVLGDIYARNKPIQQYQLTDNNGCGKLIKQDFNTMKETGT
                     WWINGSSQNNPFSGTWGMLEVFRPNPGSHERIQRFTTSTGYMAVRENGFDNNWRPWRY
                     LVQQSKSTNNSDYVALLKSESTPTPWQNAILQNGWNHHRDYGGVQFSKTFDGVVCFKG
                     TCKGGKIARESIILTLPEHFRPSTTLFKTALNNDYGSAVIGIYPNGNVVVKSNVDATW
                     LNFDNVFFKI"
     gene            32781..33044
                     /locus_tag="VWZ30_000103"
     CDS             32781..33044
                     /locus_tag="VWZ30_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333929.1"
                     /translation="MLKVTKTRQLVTEFFAQDGDQQKLVKTTVINTDNKAVSTISETL
                     HDPELYANNRISMRKHEQELREMRYKIEDAILAELEADAEHKE"
     gene            33054..33470
                     /locus_tag="VWZ30_000104"
     CDS             33054..33470
                     /locus_tag="VWZ30_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU9333930.1"
                     /translation="MKIEFFNFLRSVVQTEDGLVLYALTLIVSMEIIDFVTGTIAAII
                     NPDIEYKSKIGINGLLRKISGVLLLMILIPASVLLPEKTGFVFLHSICLGYIAFTFQS
                     LIENYRKLKGNVTLFQPIVKVFQRLLEKDDDTKKGE"
     gene            33474..33806
                     /locus_tag="VWZ30_000105"
     CDS             33474..33806
                     /locus_tag="VWZ30_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEU9333931.1"
                     /translation="MQQITEIITNGAISILVVLAGVVVRAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVASETGFKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            33810..34766
                     /gene="lytA"
                     /locus_tag="VWZ30_000106"
     CDS             33810..34766
                     /gene="lytA"
                     /locus_tag="VWZ30_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU9333932.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHWR
                     KDPELGFFSHVVGNGRVLQVGPVNNGSWDVGGGWNAETYAAVELIESHSTKEEFMADY
                     RLYIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKQDIENGLRAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSD
                     RWKKHTDGNWYYFDQSGEMATGWQKIADKWYYFDVEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(34976..35215)
                     /locus_tag="VWZ30_000107"
                     /pseudo
     CDS             complement(34976..35215)
                     /locus_tag="VWZ30_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
     gene            complement(35212..36537)
                     /locus_tag="VWZ30_000108"
     CDS             complement(35212..36537)
                     /locus_tag="VWZ30_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9333933.1"
                     /translation="MDLLGFGTVIVHFLIISHSYRLICKGRINRKELYVFGAYTLLTE
                     IVLEFSFYLLYLDKIGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            complement(36558..36683)
                     /gene="comC"
                     /locus_tag="VWZ30_000109"
     CDS             complement(36558..36683)
                     /gene="comC"
                     /locus_tag="VWZ30_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEU9333934.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRISRIILDFLFLRKK"
     gene            complement(36848..36921)
                     /locus_tag="VWZ30_000110"
     tRNA            complement(36848..36921)
                     /locus_tag="VWZ30_000110"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36885..36887),aa:Arg,seq:tct)
     gene            complement(36965..37444)
                     /gene="rlmH"
                     /locus_tag="VWZ30_000111"
     CDS             complement(36965..37444)
                     /gene="rlmH"
                     /locus_tag="VWZ30_000111"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU9333935.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     FTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            37627..38808
                     /locus_tag="VWZ30_000112"
     CDS             37627..38808
                     /locus_tag="VWZ30_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9333936.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGEIAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLDKSSGDLE
                     S"
     gene            38866..39624
                     /locus_tag="VWZ30_000113"
     CDS             38866..39624
                     /locus_tag="VWZ30_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU9333937.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            39837..41198
                     /gene="dnaA"
                     /locus_tag="VWZ30_000114"
     CDS             39837..41198
                     /gene="dnaA"
                     /locus_tag="VWZ30_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU9333938.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            41357..42493
                     /gene="dnaN"
                     /locus_tag="VWZ30_000115"
     CDS             41357..42493
                     /gene="dnaN"
                     /locus_tag="VWZ30_000115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU9333939.1"
                     /translation="MMHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            42558..42752
                     /locus_tag="VWZ30_000116"
     CDS             42558..42752
                     /locus_tag="VWZ30_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEU9333940.1"
                     /translation="MYQVGNFVEMKKPHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFDRKMNKIID"
     gene            complement(43183..43329)
                     /locus_tag="VWZ30_000117"
     CDS             complement(43183..43329)
                     /locus_tag="VWZ30_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333941.1"
                     /translation="MIEDFLIDLDNIQLEDMEIDFNMDIDIDLNIDFDLDGLDLTLLQ
                     ELEA"
     gene            complement(43383..43775)
                     /locus_tag="VWZ30_000118"
     CDS             complement(43383..43775)
                     /locus_tag="VWZ30_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9333942.1"
                     /translation="MELDKFKTMMNVRERMTYFLRFQRMAGSENQVTIDEEAWKLVLP
                     DQWNLTSKHEKAIREGLEIFAQDINSIENKRARKYFIIHYCYMRKKTMSECVEMAATS
                     STSYHRYKQIAVLNFARIHQNGELEVYK"
     gene            complement(43932..44342)
                     /locus_tag="VWZ30_000119"
     CDS             complement(43932..44342)
                     /locus_tag="VWZ30_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEU9333943.1"
                     /translation="MEAVSSALFGYSYILYPTIKQGLYLELAALESGIIKKQELSNTR
                     VQTSRVNTTENNLINVLKLKEYTLQRIERLTEERMEISRLIDKLANPLERSVLRLFYL
                     NVLDAWQVAEEIDKSKTAVYIIRQKAIEHLANVG"
     gene            complement(44754..45029)
                     /locus_tag="VWZ30_000120"
     CDS             complement(44754..45029)
                     /locus_tag="VWZ30_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333944.1"
                     /translation="MFSLSRESEHDLTHGILEVVERYLEAREKVPPRLTKLINRVELK
                     EELKISDNTLNKWESQGLRRYQPPDDGARIIFYLVDDIRRFMGVENG"
     gene            complement(45022..45363)
                     /locus_tag="VWZ30_000121"
     CDS             complement(45022..45363)
                     /locus_tag="VWZ30_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9333945.1"
                     /translation="MSLSENDKRVLRLIKVGAENSITGSEISLTTKLTERTVRDIIKR
                     LVVKHNIPIVGVRCGVFSGYFIPANKGELLDGAKAFYNQVQEESKRLAVLMNSDLESY
                     KETLKEVGGYV"
     gene            complement(45344..45637)
                     /locus_tag="VWZ30_000122"
     CDS             complement(45344..45637)
                     /locus_tag="VWZ30_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333946.1"
                     /translation="MSYTMNLYPDIEKIPDEPYFTKDIFLATCMDNATKLAVTMSLLT
                     EQTLDLETETAILKTLDTIIETQKDILQGVASGQITFVNKSKGEEGLDESIRE"
     gene            complement(45634..45849)
                     /locus_tag="VWZ30_000123"
     CDS             complement(45634..45849)
                     /locus_tag="VWZ30_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333947.1"
                     /translation="MNELDISNTHAAVLIVILIGLLLYLNHRDRKKSAQIERENQQTI
                     KTPSEDLNPDYGRYIQLAGVRVSGGME"
     gene            complement(45851..46051)
                     /locus_tag="VWZ30_000124"
     CDS             complement(45851..46051)
                     /locus_tag="VWZ30_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333948.1"
                     /translation="MTLDLGKMTQAEFDEVMADIKARNPNLLQLITDFLDRKVTPEEV
                     DDLLNMERAEQVEYIKNYQARA"
     gene            complement(46063..46563)
                     /locus_tag="VWZ30_000125"
     CDS             complement(46063..46563)
                     /locus_tag="VWZ30_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333949.1"
                     /translation="MISDKLEAISTNLEEIQKSLVGAETVLAKREISRVLNDLDDVYS
                     ELTSTEYHEQQQTIKEQTEKMKQRGIAEVIGELEEREESYYRNFWGDTQFIRLLSNFD
                     GFLFLNTYLAEITKENTYPMEQSQLLNYVWEVIAVDIAKKKRGKKNVLDSWTDSSLYT
                     RGMMID"
     gene            complement(46997..47146)
                     /locus_tag="VWZ30_000126"
     CDS             complement(46997..47146)
                     /locus_tag="VWZ30_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333950.1"
                     /translation="MEAKTHFARFMRRGMELARQLHSKEIQRDEFDRAWKRLGDQIEN
                     ETKKN"
     gene            complement(47121..47576)
                     /locus_tag="VWZ30_000127"
     CDS             complement(47121..47576)
                     /locus_tag="VWZ30_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333951.1"
                     /translation="MRKKHQVVKFKDIAEKLPELEGKRLEEITKVLGYRNLESCRTNL
                     CKLKQNNRLDFTLEKGVYSKFALLDGTVKEELEDKELSERGRYLKSVDRYKAMLNAFS
                     IAFDSTVKAETRQKAEHDGLKALDRIPDKHYALLYDMMEGEDGSKNPLR"
     gene            complement(47731..47901)
                     /locus_tag="VWZ30_000128"
     CDS             complement(47731..47901)
                     /locus_tag="VWZ30_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333952.1"
                     /translation="MGKTLGRPKSDNPKNKQLKIKMTEQDFNNLEELAKKKNMTKTDI
                     VMRGIELVKSEP"
     gene            complement(48130..48390)
                     /locus_tag="VWZ30_000129"
     CDS             complement(48130..48390)
                     /locus_tag="VWZ30_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333953.1"
                     /translation="MENEFKTVTNAKGLEIPKYPKDFKKLVEMDRQLAEYLCMNYENL
                     DNEDLGAFLETVEQGFSWILDLIESKDLLYSPQTGKKARKRQ"
     gene            complement(48402..49007)
                     /locus_tag="VWZ30_000130"
                     /pseudo
     CDS             complement(48402..49007)
                     /locus_tag="VWZ30_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518114.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            complement(49097..49330)
                     /locus_tag="VWZ30_000131"
     CDS             complement(49097..49330)
                     /locus_tag="VWZ30_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9333954.1"
                     /translation="MEQWTLKACRVNAGYTLRQIAKKVNKNFQTVSKYEKDSTYIPFE
                     LLKELSAIYQVKLDNIFLGDSTKKIELNKREVQ"
     gene            49445..50401
                     /locus_tag="VWZ30_000132"
     CDS             49445..50401
                     /locus_tag="VWZ30_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9333955.1"
                     /translation="MAKNSPQDLENREYFSKRLNLLMEQKGVRQIDLHNALDIPKSTI
                     TGYVKGKSLPTAGNLQKIADFLNVKKSFLDLRFIDTVSTSTLKELLDNNQREKVFEMF
                     FNKIKEILSFKKDYMNMEYIRGKIAFDQGVLGRVLQSSIINPGIVEIFNGLDLSDISK
                     NNEALLLSKQREAREYAYNNVLTMITNEYITRLINVIEREQALNTSYVHSEISNLINS
                     LNNKIDSYGAVVFEVNEIIEKIGIQDRKILGYCLQRKISSVQNEINDLVHNNTNNSFC
                     VTKNKIFTEKSDIIDTIEFDYYKGIQNRLNDLAEEIREHFSD"
     gene            50550..51554
                     /locus_tag="VWZ30_000133"
     CDS             50550..51554
                     /locus_tag="VWZ30_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9333956.1"
                     /translation="MTDLTTSQIERQNILNNSLALQKAEEILKVPGFYFEDTFYFTNS
                     QLATFFEVDIRTIERLVEAHKTELTENGYHTLRGEKLAKFKENAFATDTNVGSKVTQL
                     SISSFRTLLNFAMLLTNSDIAKQVRNTLLDIVINVLTEKTGGHVKFINQRDKNYLSTA
                     LEEDNARKIFTNAIDKYVSGSNFKYGQLTNEIYKAIFKERASEYKKILKLNKNDNARA
                     TMYSEVLIVIASFEKGVAYEIEQKSKSSNQKLTNTDVIEIIQRLANHPMQEPHLMHAR
                     EKMASRDLSFRNAKHEKLAEYIRPISEEDFERFIGEQSKSLSEQIDEHRQVFERLRDK
                     "
     gene            51554..52006
                     /locus_tag="VWZ30_000134"
     CDS             51554..52006
                     /locus_tag="VWZ30_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEU9333957.1"
                     /translation="MDKINYFSAEEAILLHDIEVIEKIGGLHGVKDKGRVFSVFEHLQ
                     NDDYYPDFETKLTVLVFNTVQFHMFNDGNKRSSIVFGIKFLTINHLDYLIPFFIETME
                     NIVLWVAMGLIDFNFLKEIISSILYYEELTEKVKEKLVQIALAEENNK"
     gene            52102..52254
                     /locus_tag="VWZ30_000135"
     CDS             52102..52254
                     /locus_tag="VWZ30_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEU9333958.1"
                     /translation="MKITEYTKKDGSTVYRSSVYLGIDTVTGKKVKTTISGRTKRELK
                     AKALQA"
     gene            52255..53268
                     /locus_tag="VWZ30_000136"
     CDS             52255..53268
                     /locus_tag="VWZ30_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU9333959.1"
                     /translation="MDFEKDGSTVYKAVEIKTYAELVENWLETYCHTVKKSTLMGTKF
                     KIDKYLLPAFGNYRLDKLTPPIIQKQVNQWAKDYNQLGKGFQEYPLLHSLNKRILKYA
                     VSLQAIPFNPARDIIVPRRKEKKGQKLKYLDDDNLKKFLTYLEQLPNTYKNFYDTVLY
                     KTLLATGLRIRECLALEWSDIDLKNGTLDVNKTLNIIKEITGPKTKSSIRVIDLDNKT
                     VLMLRLYKARQSQIGKEMGLTYEKVFSNSFDKHIDARMLSFRLVKHLERAGCPRFTFH
                     AFRHTHASILLNAGLPYKEIQTRLGHAKLSMTMDIYSHLSKDNQKNATSFYEKAIEKL
                     KSS"
     gene            53388..54503
                     /gene="ychF"
                     /locus_tag="VWZ30_000137"
     CDS             53388..54503
                     /gene="ychF"
                     /locus_tag="VWZ30_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU9333960.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLIESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYESEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            54574..55143
                     /locus_tag="VWZ30_000138"
     CDS             54574..55143
                     /locus_tag="VWZ30_000138"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU9333961.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            55144..58659
                     /gene="mfd"
                     /locus_tag="VWZ30_000139"
     CDS             55144..58659
                     /gene="mfd"
                     /locus_tag="VWZ30_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU9333962.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTNTRTKGNAELILLIDAYLPDTYIS
                     DQRHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQ
                     RVERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDY
                     EILEGLLIFGESLLEIKESKEKNSI"
     gene            58717..58983
                     /locus_tag="VWZ30_000140"
     CDS             58717..58983
                     /locus_tag="VWZ30_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU9333963.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            58976..59344
                     /locus_tag="VWZ30_000141"
     CDS             58976..59344
                     /locus_tag="VWZ30_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU9333964.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            59464..60732
                     /locus_tag="VWZ30_000142"
     CDS             59464..60732
                     /locus_tag="VWZ30_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEU9333965.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLLQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            60729..62006
                     /gene="tilS"
                     /locus_tag="VWZ30_000143"
     CDS             60729..62006
                     /gene="tilS"
                     /locus_tag="VWZ30_000143"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU9333966.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVENLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEEEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            62010..62552
                     /gene="hpt"
                     /locus_tag="VWZ30_000144"
     CDS             62010..62552
                     /gene="hpt"
                     /locus_tag="VWZ30_000144"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU9333967.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            62568..64526
                     /gene="ftsH"
                     /locus_tag="VWZ30_000145"
     CDS             62568..64526
                     /gene="ftsH"
                     /locus_tag="VWZ30_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU9333968.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLVGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
BASE COUNT        21867 a        10949 c        13085 g        18647 t
ORIGIN      
        1 tttttattat gctttcattt tagacgaaca tctaaaaaaa gtcaagcgaa aagataaaaa
       61 atagttaaat ttttagttgt ttcttattta caaaataagt aaaaaggagg aacacatatg
      121 ccagatatca caaatggtcg tgagaaagtc aatgattttc tgaaagacaa aggtattaaa
      181 aaaacaagtc tagcgattgc ttatggcttt aaacgacagg aagtaacaaa cattctgagt
      241 gggacgacga agggaccacg agcgaacagt ttcattcttc aagtgattga agattatggg
      301 attgagtagg agggaataac atgaatgaag ttattaaagt gactgtgaat gacaatcacg
      361 agccgattgt atctggtcgt caattgcacg aggcgttggg agttaaaaca gaatataaga
      421 agtggtttag tcgcatgact gaatacggct ttaatgaaaa tgaggacttt ttaaaggtga
      481 cccaaaaatg tctcacctct tcaacaggtc aaaatacgac tgaccacatc atcaagctag
      541 acatggccaa ggaaattgct atgatccagc ggacggagag aggcaagcaa gtccgacaat
      601 actttatcca agtagaaaaa gactttaata gccctgagaa gattatggca agagcattgc
      661 tcatggctga tcagaaagtc cacaagctgg aggctcagat tgaagcggac aaacccaaag
      721 tcctctttgc agacgcagta agtgcaagcc atacatctat cttggttggc gaacttgcca
      781 agctcattag ccaaaacggc tacaaaatcg gtgccaatcg cctcttttct tggatgcgcg
      841 aaaatggcta cctgattaag cgcaaaggct cagattggaa catgccaacc caacgtagca
      901 tggacttgaa actctttgaa atcaaggaaa caaacgtgca acacgcagat ggacatatca
      961 ctgtgaacaa gacaccaaag gtcacaggca aaggacaaca gtattttatc gataagttcc
     1021 ttaattagga atacctgaca ggttagaaag tagaaagaat gattgaaaat aagtgaagag
     1081 aaaggagact gtatgacaga ctttaaaaat ttagattgtc aatttatctt tcaagaatgc
     1141 gactgaaaat tatactgctg ttagtaatag ttttatcaac gatcctgcgc tggattttac
     1201 agcggttggc atcatgatgg tagtgctggc taatcaccca aattggcaag tctatccgga
     1261 tgagatagct aaaagaaaag gtgttaaccg aaagacaatc gataagtatt tcaaaatctt
     1321 tgaagaggct ggatatttac gaaaaatcag aaaaaaacct cctggaaatg gagggagtca
     1381 tatattcaga ttcttttcag atgtaaaaat atctgatttc caattcgata ttatgaaaca
     1441 gagattgaac ctatctatca aaagggcgtc tatgaattat aattctgaca ttccaaaaag
     1501 tgagatgtca gaaagtgaga tgtcagaaag tgagatgtca gattttgggc actaataaat
     1561 actaactaac aacaagtatt aaataacaat aaatactaac taacaacaag tactactctt
     1621 aataaataaa agagagtata caaaaaagta tctgagaaac tcagacactt tctaaaaaaa
     1681 tctaacttaa ttatagcatg aaaggggaaa aatggaaaca gttcaaatcg tgagaattaa
     1741 agatgtgatc atcgagaaga tttctgcaaa cgatgaagaa ctagagcgca tctttggatg
     1801 ttcaaaacgg caagcgggag atatgaggcg agagatgaaa aaattgccta gtcagcaaaa
     1861 ataccttaga aacgatggtc agcttgtcac aatcaaaggt tttgatgctt atctgcaata
     1921 tcgaggcagt caatcatgga agaaagaaat ggctaaaacc gttaagatga cacgatagca
     1981 gaataataac tactaacaaa taacaaactc atacttatag ataataaggg aattacagag
     2041 tttttaaagg aggaaagaaa tatgccaaat tgggcagagg gaactcttaa attaagaggc
     2101 agacgcgaaa acgttgcatc agctttaaaa gaaatgctat taggagatga aggcgcaacg
     2161 cttgaagaag aatacgatgg cactctacta agatttaaaa acgagtatga ttatttttat
     2221 ataaacggta caagacgtgc gtttatttct agtaaagata ttgaaatttg gttggatgat
     2281 gattttgtga ttatcgaact tgaagatttc gaacaagcat gggcagcatt agctgacaat
     2341 tacacagaaa tttctagtaa gtttgatgtt gatattaaaa ttttcacttt tgaaatgggt
     2401 atggaattta cacaggaaat tgaaatttca aaaggtgaaa tcatcaagaa tattgtaaac
     2461 gaaaacttta ctaactattc atgggatgtg ccttttagta gacttggagg atagataata
     2521 tggctgattt aacatttgca gaattacagc gaaaaatgca aatcgaaaaa caaacgaaac
     2581 agggagtgaa atatccgttt agaaccgcag aggacataaa taataaattt aagtctttgg
     2641 atagcggttg gagtgtatca tttccagaag atgacatcat tcaaaaaggt gacaaactgt
     2701 attataaagc ggtagctgtt gctaaaagag aaagtgatgg cacgattgaa aaagctattg
     2761 gatgggctag agaagaagat gtaccaattt ttcacacaca aaaaggggat gtgaaacaga
     2821 tgcaagatcc acaatggaca ggtgcggttg gttcttatgc tagaaaatat gctttacaag
     2881 gtttatttgc cattggaggt gaggatgttg atgagtatcc agtagaagaa agccaagagc
     2941 aaggacagaa taatcagcaa cagaaaccaa acaaccagca agcccaagga caaaatcaag
     3001 taaggtatat tgacaacatt cagtatcaag aaattaacga ccttataaat gatattgcaa
     3061 aaattaaagg gatgccgttc gatacgcttg ctaactatgt actatctgaa aaattaaaag
     3121 gtttacaaga ttttcataga gtacaagttg gtgactacga ggtattgaaa aactatttaa
     3181 ctgaacaact agcaaaagca aaagcaaaga gaggtaatta aacatggtaa aagatgtaac
     3241 taatagcttg acagaaatca aggtggattt tcaacctgca gtaattaatg ttgaccgtga
     3301 ggcgatcgag gcacaagtag ccgcagccat tgcacagtat agcggtcgcg aggttactgt
     3361 tgataattac aaagaagttt atgaagagcg aacccgcttt aataagctga ttgggggctt
     3421 agacactcaa cgcaaagatt ttaaccgaca aatcaatgag ccggcaaaag actttgataa
     3481 gtgggtcaaa gaaaaagtca tcaagcctat tgagggcgta acagatgcta tgtcagcagg
     3541 acttaatgcg attgatgaac atgaacgatt gatgcgcgtg gatgtcgtgc gtgctacatt
     3601 tgaggataag tgtatggtcg cagggattga aaaatccaca ttcgctgaca aatacgatga
     3661 gtacagcctc aagaaatatt ttaaaacagg caagtatgag ctgaaaaaga caacacttga
     3721 tgaaatggat ggcttagtac tttcagaatt tgatgccctg gaagaataca aggctaacaa
     3781 gcaagctatc caagagcaag ctcaagagta cgatttgcca gctgatagct atatcagaca
     3841 tcttgaagat ggtaagagtc ttgttgatat actcaagatg atgaaaactg accgagatgc
     3901 tgagattgca cgcaaagagc agaaagaggc tcaagaaaaa gcaaaagctg aacgacttga
     3961 agaaattgct caatcggcca agaaaaatgc taatgcgaat atcaaggctt acgatgccga
     4021 aacaggcgag attttggaac agggtacaat tacaccagaa cctcaaaaca atgcgcgaga
     4081 ggtggcaaaa tttgagccta gcgagccttt ggtcaaatta gtacgtcttg aattgcacgg
     4141 tggtttagaa cagtgggaaa atacacaaga atattttgag gataacttta tcggttttga
     4201 aactttggag gattaagtag aataaggagt cagacctatg agatgttttt atgtcagcgg
     4261 taaaattgca gatcttgatt tggggtcaga aatcaatgca gaaaattcat ttatggccgc
     4321 tattgagttt gtgaaacgat ataccggctt attaaagttt ggttcaaatg aaatcaaggt
     4381 atcagaagta gaggaggtgc aaaatgataa ataacgttgt tttagtaggg cgacttacaa
     4441 gagatgccga actgagatac acgcaatcta atattgcggt tgctacgttt actcttgctg
     4501 taaaccgtcc atttaagaac gaggctggag agcgtgaggc tgattttatc aattgcgtta
     4561 tctggagaca gtcagctgaa aatcttgtta attgggctaa aaaaggttct cttatcggag
     4621 ttacaggagt aattcaaaca cgtagctatg ataaccagca aggtcaacgt gtttatgtca
     4681 cagaagttgt tgccagtaat tttcaactgt tggaaagtcg taacagtcag caaaataatc
     4741 aaggtcatca agaccatcat ggcggttatc agcagcaggg ctacagtaat caaggcagtt
     4801 ctttccaaaa tggaaatagt tacgggcaac aaggcagttt ccttgagggg aacacaacaa
     4861 atctagttcc tgatttcacc cgtgataaca atccatttgg cagacccaca aatccattgg
     4921 atattagtga tgatgattta ccgttttagc gaaaggggat attctagtta agttatgaaa
     4981 ttcttagact tatttgctgg cattggaggt tttaggatcg gcatggaatc agccgggcat
     5041 gaatgtatag gattttgtga aatagacaaa ttcgctagag ctagttataa agctatacac
     5101 gatacgaaag gagaaattga attacatgac attacaacag tatcagatga cactattcga
     5161 ggaatcggac atgttgatgt tatctgtgga ggatttccgt gccaggcttt cagcattgca
     5221 ggaaacagac gaggatttga agatacaaga ggaactctct tctttgaaat cgcaaggttc
     5281 gcatctattc tcaaacccag atatctattc cttgaaaacg tcaagggact cctcaaccac
     5341 gacagaggaa atacctttga ggtcatcctc tcagcgttgg atgaactggg gtatgatgtg
     5401 gaatggcaag tgcttaacag caaagatttt ggagtccccc aaaatcggga acgtgtgttc
     5461 attatcggac attctagaaa aagaggcacc agaagagttt ttcctatcgg aagagcaaat
     5521 agagaatttg attttgaacc aaaaataaag attgttggta acactaaaaa tccgaacggg
     5581 acaagtcaag ggactgggag cgttgtttac gactcaaacg gtttagtcgg tacgctttgc
     5641 gctagagatt ataaagagcc aaaacaagta gctataccag tattgacacc tgatagagta
     5701 aataaacgac agaatggtag acgtttaaaa aaaaacggtg agcctatgtt tacgctgacg
     5761 gcacaagacc gtcatgggat tttaattaaa gaagcaacaa aaaaaggtta tgcagaggct
     5821 acagttggtg atagtgtaaa cctatctcat ccaaactcta aaacaaggcg aggtagagtt
     5881 ggtaatcaga tagcaaatac tctcttaact ggagagagtc aaggtgtggt tgagcctgat
     5941 tttaggatta ggaagctaac acctaaagaa tgctggaggt tacaaggttt tcctgattgg
     6001 gcttttgata aggcgcagga ggtcaactct aacagtcaat tatacaagca agtaggcaat
     6061 agtgtaacag taaacgttat ttctgcaata gcacagggtt taggatgaaa ttaatcctat
     6121 ttaaaccaat ttgaaaagga aacagaaaaa aatgacaaaa attgaaatcg ttatggtact
     6181 tacaactttg atgtctatca catgggcagc gattgttaca attcacgcta tgcaagctat
     6241 taaaaagcac aaggcaaaag tggattatta tcagaaacca caagtgcaat gtgagattgc
     6301 acgtcatgta cttaaaaaca aatggtactc agatggtggg gaggtgttta gatgaaagta
     6361 tttgatggcg ctatttcaaa ttagcttaat ttaaagcagg aggacaatat ggataaaaaa
     6421 cttattgggt tagatctaac ccacattgca gatggaggat tacaggagaa actagacaaa
     6481 gagcttgaaa aagtctttga taacatcctt gacctaaata cagatgcgaa agcaaaacga
     6541 aaagtgacta tcacacttac aatgtcagca aatgaagagc gtacagttgt tgatactacc
     6601 atggaggtga aatcaaaatt tgcgcctcaa aatggagtag ctacaacaat tcttattggg
     6661 cgtgattttg atacagggca agtacatgct aatgagctaa aaagtacagt acctggtcaa
     6721 atgtactttg atgaaaacgg agaaattctg acggatattg ggcaaccagt ggcagaaatt
     6781 gaacaacaag cagaaacaaa accagatatt attgatttca acaaaaagaa agtaggtaac
     6841 taatatgaca acagaaaatc ttaaagcagc attggaatac gcagtagaac taaatgagca
     6901 tggtttggaa attttaacag ctgcagatgg aacagagtat tatgatgcca acaaattcaa
     6961 cctcaaagaa cttgacccta aacgctatcc taaaactctg gagctatcaa ccttgacaag
     7021 ccttgttgac tatctcaaaa cagacctaaa caatttgaaa aaccaacgct tgattgtagc
     7081 agttgagaaa aacgatgagg tttgtgtgtg gtctgaaaat gatgagatcg aacatcgcac
     7141 attgcttgtt gatgttaagg cacgcatccc agagttatct tttggccgtt tcctatcatt
     7201 ggaacagttc aatatcatgt tgcaatcaaa ctttattgac gataacgatc gtggcacatt
     7261 gctagaattt gctagcgcat tgaaaattga gaatggggct gaaattgaag ataatggagt
     7321 atctcaagta gcaacagtta aaacaggggt ggcaagcctt gctaaaggca aagcacctaa
     7381 tccggttaca ttgcgcccat atcgtacatt tagcgaggtt gagcaaccgg caagcctatt
     7441 tgtctttagg attgataagc aagccaatat ggctttattt gaggcagatg gtaagcgttg
     7501 ggtagctgat gcagtaggaa acattgcatc ctatctaaaa gagcaactag cagaccaaaa
     7561 acatatcaca gtattagcat aagaaagtgg aaattaaaat gagtgattac aaacaacgga
     7621 tgattgaaga atacaaacaa ttaaaagagc gcaccaataa gttaagtttg atgattagta
     7681 actattacgt aggaacactt gattttaaac taaaatgtcc tattgagtta cttgaaactc
     7741 aacactatac aatgtgtgca tatctcaaga tccttgaaca gcgtgcagaa attgaaaaca
     7801 ttgagtttta aggtaatcaa aatgaaattt gagttttctt tgcctcggaa tactaagcta
     7861 aaatctctaa acatggttat caatagtaat gacagacaac atcaaacaga taaggctaaa
     7921 gttactaaac gcattagagc ttttgcttat tggcatacat cgatgaacaa ggataaagag
     7981 agggctgctt ttagcccctc caacccttgt gaggttacag ttacaattta cagccctact
     8041 aagtcaaaac ttgacccacc taatttatat ccaacagtca aggctatcat tgatggcatg
     8101 actgatgcag gtatttggac agatgataat cataaggtta tcaaaaagtt atcctttgtt
     8161 tatggtggat taagcgagga aaaagggcat tatagattag agtttgatat agaggaggtt
     8221 taaagtgatt gaagttaata taaaattcga taattttgaa gcgcatggct tttaccaaga
     8281 tgatactaaa ctaggaaaaa ttagagatgc aatcatatct caaatgaata atgggcatgt
     8341 ggttgtttta ggggaagata gaggtattct attaaatcct aaagttataa aaagtgtaca
     8401 atttaaggtt gtagaagata accagatcta atcgtttttt gacctttaga gaatctgagt
     8461 tattttgagg agttggaaga tgatggaaga gttaaagcaa aaagttaatg cagtatacaa
     8521 ctggacggta gaagacggga aacccaaacc tcccaagcaa gatttaccac aagcagtgaa
     8581 agaccgggtg gactattttt gggaaatggc agaagatggt atgacgttta tgggagcgat
     8641 ggaatgcatc ttcgctgatg aaaagcctac cgactatgat ttgggagcta ctaagggttg
     8701 gttgccaaaa tctaaggagt ttgatgattg gattggctat gcgccaagca tggctcaggt
     8761 agttattgca gtttatttga tttatggagg aaactaagat gaatattaaa gcattgatta
     8821 agaagtatga agaattgtgg aatgaacaca gcccttttta tgaacctgta ccttatactt
     8881 caatggttga actttttttg aaagagttga aacaactaga cgaaccagaa aaagtcaaag
     8941 ttccgcagtt tatggcggat tggattgatt atttcaaaaa aaatggtgac tgggatttgt
     9001 ttcaagcgat ggattatttg tttggaaaaa aagaaataag ggaatggctt gagtataaaa
     9061 acaaccaaga cctcttcgct cgtgcttggc ttgacggcta cgaggtcgag aaagagaagc
     9121 ggtatttggt gactttaaaa aataggcagc ctttggtcaa atcgcaatca gggagtactc
     9181 tttattttag tcaagatata acagctagga attataaagg tactcaaaaa gaactagaag
     9241 acgcaaagtt cggctgggta tttgattgcc cagggattga gattgaggag gtggagtgat
     9301 gacacaaaca cttgaagaag gaatgaagaa tcaaagtaaa tgcataaaaa tcccaatgga
     9361 aatcagaccg tttgatgtgg gttatcgaat agtaaataaa cacggtcaag cgcttgcttt
     9421 aaaaaacgga gcaagtatat tcgatttacc atcactagct gaaaaagcta ttaagaaaga
     9481 gtttagtaaa gatgacccag attttgatat tgaaaaacat ttcgttgaag aggttgctat
     9541 tgtcaattta agtaaatttc atagttattt tgaggaggtg atacatgact gatgacgaag
     9601 aaaaaaatag agcgcttgtc agttatccat cgcagggaaa tcaattggct aaagtggtat
     9661 tttttgaggg ataagaaaaa tccaaagaga accattttgg agcaaaaaat tatagtttct
     9721 catatcaaaa ctgataggct tgaagctaag tttttaagca acttaaaaaa atcaactgaa
     9781 gattttatag ataagtctga tcctaaatat ttgcgggcaa taaaagaggt ttatgtttac
     9841 gaaaacatga atgtcattgg agcttgtcaa aaaatactat tttatagtcc gactcaagcc
     9901 tatgtattac ttaatgcgtg gtttaacgat tattttcgtg cgacttacac agaattacta
     9961 gaaaacgcca tcttagataa ataaccgtaa aaaatccaaa gcttatgtat ctataatcaa
    10021 gatatataag ctttttcgaa aggagagata tggagatgtt acagattgaa tatgtagata
    10081 taaaatccat taaaccatat cacaaaaacg ctaggcataa tgacggagag gcaacagaga
    10141 aagttgctgc atccataaaa gcttttggtt ttcagcaacc tatcttagta gatgataata
    10201 acatcattat tacaggacat actaggctaa aggctgctct ttctttaggt atagacacaa
    10261 tacctatcgc tcacgctgta aacctcacag atgagcagat aaaagcttat agactagcag
    10321 ataatcgagt tgctgagtat tcaacgtggg actcagaatt attaaatatt gagctttctc
    10381 aatttgaaac aatagatatg gctcagtttg gttttgagtt atcagttaca gggttcaact
    10441 ttggtaacga ggaggagcag caagaggaaa ctgaaaacga ggaggaagat gctgaggatt
    10501 ttcacagaga cacaactata aatcagtaca atctttttca ttatgacgat acaagggttg
    10561 agggttttta taacatgcct aaaatcgagg gcgtggatca tattcctaaa gattttcaag
    10621 gctttaatta tgttttaaat aaaccagatt acagctcatg cgtgcatttt ttcctagatg
    10681 attatcaatt tgaaagaata tggcaaagac cagactttta cattgaaaag ctgctagaat
    10741 ttgatagcgc cttagctccg gattttagct tatatcttga tatgcctatc gctatgcaag
    10801 tatggaacat ttacaggtca aggctgatag gtcagattat gcaagattac ggccttacag
    10861 ttatccctac tgtatcgtgg gctagtgagg aaagctttga tttttgtttt gacggcttgc
    10921 ctaaaaactc aacgctagca atcagtacaa taggcgtaaa gcaaaacaaa gagcagtttg
    10981 aggtatggaa aaatggagtt actgagatga taaaacggtt gactccaaaa agaattgtag
    11041 tatatggcgg aaaagtggaa tacgattata aagatataga ggttgtatat tttgaaaatg
    11101 caacaacgga aaggatgaaa gaaagtggca caaaaactaa ctaaactaaa agatattttt
    11161 aaacatgttt caagtattga tctaggtaaa gagattttat ttgaagatct tgagctttac
    11221 aataaagaaa cagaaacaag caaacaatac caatctatcg aggaggcaga aaatgaccta
    11281 tctttgatgg aaaaagtaaa taaaatcaat ttcactctag gcggtggacg tggtgcaaat
    11341 tttgagaaag ggaaagacgg taagtatccg ggttttagag gtgctggtgg tgcaagagat
    11401 agtgggagct caaaagcctt acatccagca tctttaaaca atcaagggcg cttttcaagt
    11461 gttgagggaa ctatccagga atttattaaa aaacacggtg gctctagaac agaatacagt
    11521 acagcagttg actctcaagg ctttgctcat aattatgtac acggtgggaa aaacagcgta
    11581 caaattttgc ctatctctgg tggatttaca gcaatacata accatccgaa tggcagcaat
    11641 ttctcaagta cagatttaca tagctttgca gcattaaaag gtatgaatac actagttgca
    11701 acgaatagct ctaaagcgta tcgaattaca aaaggggcta actttgatgc taaaggcttt
    11761 gataaagctg tgagcaagtc acgttttact acaaaagatt acaataaagg agctgaccta
    11821 tggctcaaga aaaacgctaa gaaatacggg tacacttact catacgagta aaagaaaaga
    11881 ggctaaggta tgggtggtag aggagcaaaa ttaaacttgt caacaacgaa aatgacaact
    11941 caaaatatag agacttgatt aacaaaatcg aaaagttatt aggaatagcg taaaacatcc
    12001 ccttttttaa catatacaat gaaatcataa gtataaaatg cttgtgattt ttttgtttga
    12061 aaggagggtg gaaattgcct agagatggaa ctaaaaattt aaaaccagtt acagaacgaa
    12121 ccaaagatga agcaagagct attagctcaa aaggaggtaa agcatctggc atagcaagaa
    12181 gaaaaaaagc tgatctaaaa aaagcatttg aaatcctctt atctttggat gtgacggata
    12241 gtaaaatcaa gaaacaactt gaggagatgg gtatggctgg caataacgag gctttgctag
    12301 cctttgcaac ctttcagcaa gctgtaaaag gcaatcagaa agcgactgag aacataatca
    12361 agctgacaaa tactaaagat aaatacgata tacaagaaca gaaagagcgt attaaagcac
    12421 tcaaatatga aaatagagag cgtgctgaag ctgagaaagg ctcaagtgaa actatcgaga
    12481 tagtggatgc atgggctgaa gatgtgaggg gggcaacgga tgacctttaa tgtccagaag
    12541 aacatcaacc ctcatttcaa atctgtttgg atttctagct taccttataa tgttttgaaa
    12601 ggtgggcgta actcttttaa atcatcggtt attgtactta aactagcgta tatgatgata
    12661 aggtatatta tcgctggaga ggcggccaat atcgttgtta tccgtaaggt ggccaatact
    12721 attagagaca gtgttttcaa taaagtttgg tgggcattga acctttttgg tatagctgag
    12781 cagttcacaa aaacagttag cccgtttaaa atcgtacaca aaacgaccgg ctcaacattt
    12841 tacttttacg gacaagatga ctttcaaaaa ctcaaatcaa acgacattgg aaatatcata
    12901 gcggtttggt atgaagaggc tgctgaattt aatgaccaag aggactttga ccaatcaaac
    12961 gtgacgttca tgaggcagaa acacccacgc gccaagtttg tacaattctt ttggagttac
    13021 aatccaccta gaaatccata tagttggatc aatgaatggt ttgagagcat caaaacgaat
    13081 aagaactatt tagctcactc aagcacctat cttgatgatg aactcggatt cgttactgag
    13141 cagatgctag aagatataga gcgtatcaaa gagaatgact acgattatta cagatactta
    13201 tatctaggtg aggcagtggg attaggtaac aacgtgtata acatgagtat gtttcatgct
    13261 attgatgctt tgcctagcga tgataagctg attggcatat catttgcgct agatggcgga
    13321 catcaacagt cagcaaccgc ttgttgcgct tttggaataa cagctaaagg taaggttata
    13381 ttattagata cttggtatta ttcaccagct ggacaagtgg taaagaaagc gcctagtcag
    13441 ttatctaaag agatatatgc ttatatgcga tcagttattg agaagtacag agtacaagcc
    13501 ttgcaataca caatagatag tgctgaggga gcgttaagaa accagatgtt tcttgacttt
    13561 ggtttgaaat ggcatccagt cgctaaactt agaaaagtga ctatgattga cagttttcaa
    13621 tctttgcttg ctcaaggtcg cttttactat ctcaacaccg aaaacaacaa gatatttatt
    13681 gaagaacaca agatgtatcg ttgggatgaa aagactatca aatctgataa tcctagcgtt
    13741 atcaaagaag atgaccatac atgcgacaca acacagtatt ttgtgttaga caatgcaaaa
    13801 ttgctcggtt tgcgtgttgg taacgtttag aggaggacga tcatgagcct atttcaaaaa
    13861 gtaaaagact tttttagtcg agggaggtat tacatgcaga catcaaatct taatagtatt
    13921 ttggaacatc caaaaattgc agtgactcaa gaggagtatg accggattaa gagaaatcta
    13981 gtctactatc aatcaaaatg ggatgatgtt cagtacaaga atacggatgg agatattaaa
    14041 tcccgtccaa tgaatcactt gccaattgca agaacagcat cgaagaagat tgctagctta
    14101 gtttacaatg aacaggcaac tatcacaaca aaaaacgaaa ttttacagaa atttttggat
    14161 gacatgctaa ctaacgaccg attcaataag aattttgagc ggtatctaga aagctgtttg
    14221 gcacttggtg gcctagctat gcgcccttat attgacgggg ataaggtcag agtggcattt
    14281 attcaagcgc ctgtgttctt tccactagaa agcaacacac aagatgtttc aagtgctgca
    14341 atccttacta agactatcaa atctgagggg cgtaagaacg tttactatac ccttgttgaa
    14401 tttcacgaat gggtaacagc agacggacaa gaaacaggta gcacaaacga taaaaagtat
    14461 tatcgtatta caaatgaact ttataggtca gatgtgaatg atgtgttagg tcaacgtgtg
    14521 aacttgagtg aactagacaa gtacaaaaat ttagagcctg taacagtctt tgaaaaccta
    14581 tcaagacctc tatttactta tctaaaaact ccaggtatga ataacaaaga catcaacagc
    14641 cctcttggtt tgtctatctt tgataacgca aagacgacta ttgacttcat caatcgttct
    14701 tacgatgaat ttatgtggga agtgaggatg ggacaaagac gagttattgt gcccgaacat
    14761 ctaacacaaa gacaatatca acgtccagat ggaacaatag attttagacc acggtttgat
    14821 gttgagcaga atgtttatat gcaaattggc ggctctagta tggatgccgg gggcattaca
    14881 gaccttacct caccaattcg agcaaatgat tatattttgg cgatttcaga gggattgaaa
    14941 ctctttgaaa tgcagattgg tgtatcaagt ggcatgttta cattcgatgg tcaaggaatg
    15001 aaaaccgcaa cagaaattgt cagcgagaac tcagacactt atcagatgcg aagtagtatt
    15061 gtcgcacttg ttgaacaatc tatcaaggag ctttgtgttt caatgtgtga gctcggtaag
    15121 gcggtagggg tttacagcgg agaaattcca gaacttgatg atatttcagt taatttggat
    15181 gatggtgtat ttactgatag gcatgcagaa cttgattatt gggctaaaat ggtagccgca
    15241 ggattctcaa ccaaaaaacg ggcaattggt aagacattaa atatttctga tgttgaggca
    15301 gaaaaagaac tcaatgctat caatagtgag ttgctaccta tgaatgatgc cgaacttgct
    15361 atttatggta tgcatgacca aaacgaggag gaagcagatg acaaaggtta aattcggagt
    15421 tactagtgtt gactactcag caagcattga agatacgcca acaataaaat taggtttaat
    15481 aattagaggg agcgggagac tagatgcctc ttcagttatt aaaaaactaa tcaaggatgt
    15541 ttctgaacta gaatacgaat tagaagaata aactggtcaa ttggccaatt ttctttcaag
    15601 agagggcttt tgaatgaaaa aaaagagaaa acagctcacg tttaacgacc aacaatttcc
    15661 tttgcaaatg caaggcgttg gggatattta cgaaaaatta cagattgata tctttgaccg
    15721 tatgataaaa cgcttaaaag agcgtgggtc tattgattta atgagaaacc cttatatctg
    15781 gcagttagag aaactaaatg atatgcacat gctcaatgaa cagaatctaa agcttatttc
    15841 agagcgtaca ggaattgctg aaagattgtt gcgtgatgta attgagaatg aaggtttgaa
    15901 agtctataag gacactaaac agcaacttga agaagatttg aataaaatac ctgagggaga
    15961 gatttcaaat ggcgttacgg acagcttaga ggcttattcg aggcaagcag ttagtgattt
    16021 aaaccttatc aatacaacat tgcctaagag cttgcaagta gcttataaat cgattgtgga
    16081 ggagacagtc gcacaggtag ttgcaggaac taaaacaagc gatgttgctt tgcatgatac
    16141 catcatgaaa tggcagaaga acgcttttac gggctttgtc gataaaggtg ggaggcattg
    16201 gaaagctgat agctatgcga gggctattat caagagcaca acatacaaag tttataacga
    16261 aatgcgtact agacctgctg aggagttagg agtagatact ttttactatt cgatgaaagc
    16321 aatggctaga ccagcttgta gtccgttaca agggcagata gttacaaaag gggctggtag
    16381 ggagatagat gggataacta tctattcatt attggattat gggtatggaa cagcagcagg
    16441 atgtttagga atccattgtg gtcattatct gacaccgttt attgttggag ttcatgagtt
    16501 accgaactta ccagactatc tgaagaatct aacaccagaa caagctgaag aaaatgcacg
    16561 cattgaagca ggtcaaagag gccttgagag acttatcaag acacataaag agcggttgca
    16621 ttacgctcat accttgcaag atgacaagat gatacaagct gagcgtttga aagttagagg
    16681 gtatcaaact aagatccgca acttgataaa tcagcatgat ttcttaacaa gagattacag
    16741 acgagagaaa ttatatattt cataaaggat ttgtgtttca caagtccttt tttgtgttta
    16801 aaaccgtaaa aaatccctat ccatcaaagg tatattgaga gagtaaataa tattttgctt
    16861 taggtgggag ttatccacct aaaaaagaac taggagggta caaatggcat ttacaactga
    16921 agaactactc aatcttgggt tgacagaaga acaggctaag tcagtctttg ctttgcgagg
    16981 aaaagagctg aatgaggaca aatcagcctt agaaactatc aaacaagagc gagatagtct
    17041 caaatcacag ttgcaaaaag cagaggagca agttgaacac ttgaaatcac ttgagaatat
    17101 cagcgctgaa caaaaagatg cgattgataa attgcaagct gaatatgaca agtataaaaa
    17161 cgaagctgca gctgaacttg cacagacaaa aaaggttagt gctatcagtc tagctctgaa
    17221 agatacaaat gctttcaatc cagacaaatt gatgaaattc attgatgttg atgctatcca
    17281 gttagacgac aacgggaaac ctcagattga tgaagtaatc aacggtttaa aagaaagtga
    17341 tccatatctg ttcaaagctg aagaaagtaa gcctagcccc aatattttac ctcaaggtaa
    17401 tccggcagga gagggtacaa gtgatgttga tccgttccaa gcgattattg acgggtatgg
    17461 caaataacag aaaggagatt acaaatgcca agtaatcaaa acaacgcagt gcgccgctat
    17521 gagaaacaat atgcgggcat tcttgagaca gtttttggag tgcgggcagc attttcaaac
    17581 gctctagcac ctattcagat tttggatggg gtacaagaaa actctacggc tttctcagtt
    17641 aaaacaaaca acacaccagt tgtaatcggt gagtacaaaa ccggtgaaaa tgatggtgat
    17701 tttggtgata actcaggggc tcagtcacgc tttggtggtg tgacagaagt taaatatgaa
    17761 aatacagatg tcaactatga ctataccctt acaatccatg agggtcttga ccgttacaca
    17821 gtaaacaatg atcttaacgc tgctgttgcc gaccgcttga agttgcaatc cgaggcacaa
    17881 actcgaacag tgaacaagcg aattggtaaa tacttgtctg acaacgctac taagacggaa
    17941 gcccttgctg attttacaga tgacaaagta aaagctttat tcaataagtt gtcggctttt
    18001 tacacaaaca acgaagttac agcgccgatt actgtttact tgcgttctga attttacaac
    18061 gccatcgtag atatggcatc agttacaagc gccaaagggg caactatctc ccttgatgaa
    18121 aacgggctac caaaatataa gggctttacc ttggaagaaa cgccagcgca gtactttgag
    18181 acaggagtta tcgctatctt ctcaccaaac ggtattgtca ttccgtttgt tggtatctca
    18241 acagcccgtg taattgaggc tgaaaacttt gatggtgtga aattgcaagc tgctgctaag
    18301 ggtggtactt acactcttga tgacaataag aaagcaattt acaaagttac aggaactatt
    18361 gtataggagg tagaacatgg cactttacaa agcaacaaaa aatcttttct ttgagcaact
    18421 caacatggat gtgattgtcg atgacattat taaacttgat gaagattatg ctaaagaagt
    18481 caacaagaaa ctaaaaaatg cttttccaga tgtgaaaaat gttttagaac ttgttgacaa
    18541 aaatggaacg cttgaaccag aagatgcccc atctgtagat gatgcatctc aggcaactgt
    18601 tgaagattaa ataaggggtg gcaacaccct ttatttttaa gggaggttac acatgactta
    18661 tttgacacaa gaggagttcg atgagttagg ttttgatgaa gttacagact ttgaaaaatt
    18721 ggcaaaacgg gcaaagatag cgattgagct atacacaaac ggtatttatc agaaagacat
    18781 tgattttgaa aaagaaattg cctatcgcaa atctgctgta aagcttgcta tggcattcca
    18841 aatagcctat ctcgatgcct ctggtattat gtcagccgat gacaaacagc tagccaatag
    18901 tgtctctatt ggccgtacat caatctctta tagcacctca caaagcacat tagcaggtca
    18961 gcgatttaat ttgtctatgg atgctgaaaa tgctttgaga caagccggct ttagcctagt
    19021 tgttggagtt gcctatgatc gataagcggt tattaaaagg gattgacaag cgtttgttaa
    19081 aggatgtcct aaccataaaa aaagtagctg ataaaaacga ttatggggat gaagtatatt
    19141 cagaaccgtt gactattaaa aatgtacgtt ttgatagatc agtgggggga tctggtaatc
    19201 gtaattcaaa aactggtaca ggtaattcaa aaactggtac aggaaattca aaatcaaggc
    19261 aaaaacaagg ggttatatac ctctatccct cgctatcttt tgtgacagtt gataacagtt
    19321 ggatgggtgc aaaagtaaac gatgggatag gagattatac aattaatgga tttcaaacta
    19381 actattatga tggtgagata ttcagtcaag aaattgaggt gatctaatga gtatcgccat
    19441 taaagttgac ttgcagaaag ctaaacagaa actttcgaac gaatccatga caagaggaaa
    19501 gattgcagtc gctagcaaaa tcttgctaaa caatgagcaa tatatcccct tgaggggtgg
    19561 agagttgaga gcttctggcc gaatcgttgg acaaggtgat gctgttgtct atggaacagt
    19621 ttatgctaga gcgcaatttt acggggcaaa cggcattgtc acctttagga gatataccac
    19681 tccaggtaca ggaaaacgat gggatcaagt tgctactagt aaacatgctg aagaatgggc
    19741 tagagctttt gtgaaaggaa tgggactttg atgcgagaga atgactttca aaatgtactt
    19801 ttaaagcata tcaagacttt aaatttacca gttgaaccac gctttgatta ctttgaggat
    19861 gacaaagatg atctggttat caatcagata ccaggcggga aagtggatag agagtatatg
    19921 gatggcacac aagaagtttc tttgccgttt gaaatcgccg taaaggcaaa aaagaactca
    19981 gtagccaatg acactatttg gttagtcacc tcagaactag caaagataga cttagtttta
    20041 ccaagtgaca ataattctta tgaatatatg ggaatggaag tcagcaggcc tgccatgaaa
    20101 ggcaaggatg agcaaggcta ttattattac acaattgaaa ttgtggcgaa aatcgtaata
    20161 gagaggaaca aacaatgaca agacaaaaaa acgccctacg tggccatttt gtagctccat
    20221 acaatggagg aactgaacca tcaacagaag atacatggtt ggaacttgct aaatggatct
    20281 cagacgtatc agatgataca gacgagaaaa cagatgatca agcatactat gacggtgatg
    20341 gagttgaaga aacaaccgtg gtcagcgtaa aaggtgctta tacctttgag ggtacttacg
    20401 atccagacga taaagcacag gctcttattg ctgggatgaa gtacaagaca ggggatgacc
    20461 gtaagctatg gcacaaggtt gtttcttctg acaggaagaa acaatgggtg ggagctgcaa
    20521 ctgcaacaga aatcaaagca ggttctggcg ctgcctctga ctatgaggcg tttggatgta
    20581 agctttctta caactcaacg ccaaaagaga ctggtattgg gtaatagctt ttgataaggg
    20641 cgggcattga gccttgccct ttttaacaac aggaaaagga gtaaagacat gacagatatt
    20701 cagattgaac taaaacgtac aggatttcca gtaaaaatcg gagaagtaga gctatggttt
    20761 gatacaagtc aagagagctt aatgcgcttt tatgacatgg aagaagaact aaaacgtcgc
    20821 cttgtccaat atgaattaga tgtggtatct gcaaatatca ataacaaaat tgagcgtgat
    20881 ggagtaacta aagaagtagt tgctggggct attgaattgg agaagaaaca gcttgagatt
    20941 caatatgatc ttatttttgg cgacggtaca tttgacaagt tatattctat atatccagat
    21001 tataacgccc taaataacgc tctagaacag accgcaatca tgttgcatga caagttggaa
    21061 gaagttgctg agcaacacaa aacggtggtg aaagagcgtg ctagtcacta tttaaacaag
    21121 ggaaaagtca ctccaatcaa gaacaacaag aaacaaaaaa agaacaaaaa gaaatagcag
    21181 gtaaaaaata tgtctatgaa attaaatgat gccttaatca caaatttttc tattgctgat
    21241 aaagagtacg acatagacct gtcttttaat aaagttctag atgtctttga aatcctaaaa
    21301 gaggatgaaa tgacgcgtct agaacaagct cagttgattg tccatttgct aactggccaa
    21361 gaattatacg acatcaaaga ggttgtagat tgttggattt acataaaaga acactttcta
    21421 gggatcgaaa aagaaactgt tcagtatgat ttgctaggca atcccatgcc aaaggcaaaa
    21481 ggtgaagaag aacaagaaaa attgattgat tttgaacaag atgcagagta catttatgct
    21541 agttttttac aagcctacgg catcaatctt ttgaaagttc aaaatgagtt gacatggaca
    21601 gaatttaaag cacttttgaa cgctttgcca gataacacaa tcatgcaaca gattatagaa
    21661 atccgagcat ggaaaccaga atatggtggg gataaaaata aaatgcgcaa attacaagct
    21721 aaatatagtt taggaaagga gggagaagat aatgtctgat ggaaaagtga ctatcgttgt
    21781 cgatgtggat ggtaataaag tcaaggttct aaacgatgag ttagataaaa cggcacagaa
    21841 aggtgataga gggagcagtt ccctaaagaa atttgcggta ggcagtgcag ttttccaact
    21901 tgccgctaaa ggtgctgaac ttttgggaga ggctttagga ggcgctattc agcgttttga
    21961 cacgttagaa agttatccaa gagtgatgca agctatgggg catagtacag aagatgtcac
    22021 gcgctcaact aagaaactag cggcaggtat tgagggtttg cctacgactt taaatgaagt
    22081 ggtaggcaca gctcaacgcc ttacctcgat tactggcgat ataaacaaat caacagattt
    22141 aacacttgct cttaataatg cctttcttgc ttctggatct tctagtgctg atgcaagccg
    22201 tggtttacaa cagttcagtc agatgttatc agctggtaag gttgacatgc aaagttggaa
    22261 aacgttacag gaaaccatgc cttatgcttt gcaaaagact gctgaatcat tcggttttgc
    22321 tggccaatct gctcagaatg atttctattc tgcattaaaa gaggggcgta tcactttcaa
    22381 ccaattttca agcaaattgg ttgaattgaa tggtggcgtt ggtggttttg cagaacttgc
    22441 taaaactaac agtaaaggga tccagacatc ttttgggaac ttaaagaatg cggttgttaa
    22501 aggtgtagct aatactatca aggctcttga cgatttaaca aaggcagcaa caggtaagac
    22561 gattgctgag aacttcgatg cattgaaagt aatcatcaat gcggcttttg gtgttattgt
    22621 caacgtaatt aaagctagca cacctgtttt tcagactttg tttagtattt tgggtactgg
    22681 agcttctgta atctcatttt tgacaccggc tattatcggt ttagttgctg ctttggtaac
    22741 tatgcgtgcc atcaatcaag cggtaaaaac gactaaggac ttgataagcg cgtggaaaac
    22801 attcaaaaca acagccacag gagcgattca gatcatcaat ctaatgacag ctgcccaagc
    22861 tacttgtggc tcagtaacaa aggctcaact ggttgctaac ttggccaata acggagcttt
    22921 gacagcatcc aatttgcttt atggggttct aactggctct atcagcttac agactgctgc
    22981 tactattgct gcgactgctg caactaccgc atttaaagca gcgctgacag ctttaaccgg
    23041 cccgattggt ttggttgttg ctgggattgg tttggttgtg ggagtatgtg tgactttgtg
    23101 gcaatggcta actgccgaga gtgaggagac caaacgcctc aaatcagaac aagaggagtt
    23161 agtcaagagt acggatcaat taacggattc tgttaaacaa agcgcaaaag aacgtcaaaa
    23221 aaatcttgag tctgtaaaag gtaatacaga atcttaccaa aaattggctg acgaaattgt
    23281 ccagttatca caaaagacaa ataagacagc agcagacaag aaaaatctta agaaaaagat
    23341 tgatgctttg aacgcctcag ttagcggttt aaatctagcc tatgataaga actctgattc
    23401 tttgtctcat aacagtgacc aaatcaaagc tcgtatatca gcgatggagg cagaatcaac
    23461 atgggagaca tcccagaaga acctgcttga tatcgaacaa aagcgtgctg aaattggcga
    23521 acagctaaag aagatagctg aacaacgcaa aaaatggaat gaagaatcca atgttagcga
    23581 tagtgtccgt aaagaaagac tgcaagaact caacgacaag gaaactgagc taaaaaatac
    23641 tcagacagaa ttgcaaactg agtacgaaaa aacgtctcaa gttcaacagg cggcatctga
    23701 agcgatggct gctgctgccg aaaatggttc aaatagacaa gttgtagcat acgaaaatat
    23761 gtctaaatct caacaaaaag caatagacga tatgcgtact aagtacaatg aattacttga
    23821 gacaactacg aacatgtttg aacaaatcaa gtataagtct gctattagtg tcgatgaaat
    23881 gattgccaac ctccaaaaaa accaagaggc ggttaataat tgggcaacaa acctcaatac
    23941 attggccgaa cgtggggtaa acgaggggat tttagctaaa ttacaagcga tggggcctca
    24001 aggtgggttg tacgttcaag aactcgttaa tgcatcagac gaaaaattgg caacattgaa
    24061 cgaagtcttt actcaaggtg gtgagtcagc tatgaatggc ttaactgctg gtatggatac
    24121 gggtgctttg ggtatcacag acaaaatcaa gggtattgta caaagtcaag tttcaagctt
    24181 acaagaggaa attgcagctg ctgacttttc tagtttggga caagaaatcc ccaacggggt
    24241 cagtcaaggg atagaacaag gagtttctac tgccggagaa tcttctaaaa acatggctaa
    24301 tgatataaaa gaatccttta cgagcgaaat ggatattaat tccccatctc gtgttttcaa
    24361 tgagtatggt ggttttatca ctactggttt agctgagggg gtagataaag gtaccaatca
    24421 acctgtatca tctgttacta atttagccaa tcaaattaag aaaccatttg atagtctgca
    24481 gagtgatttc acgtacattg gtgaaatggc gatgtctggt cttaatgcag ggctttggag
    24541 tggctctggt tctgttatgg caacagctaa ttcaattgct gaaagggtaa aagcgaccat
    24601 caagagcgca ctagatattc actcgccatc tagagcaatg cgtgatgaag tcggacgttt
    24661 cattcctcaa ggtatcgctg ttggtattga agcagatgca ggggttgttg aaaaatcaat
    24721 gttgcgatta aaagaaagca tgatgattga tactagacca gaaattgcac ttggcttaaa
    24781 caagaaacta ggtgctcaag tgactgttaa acaaagtagt aagcagacaa tagctgaaaa
    24841 aatcaaagtt actatggaca agtctagcga actactcgaa aaagccctgg atgtagctga
    24901 gacggccgtt agacgaccaa atgaaatgta cttaaacgat ggtactttag tcgccagaac
    24961 aggcgataaa tttgctaaat atcaatcgga acaactaaga cgagataata ggatgaaagg
    25021 ggtattgtca tgacaaagat aatgactttc aacggagttg atatgtctaa attctttcgt
    25081 ataacagata ttatccgccc tatcgggaac aagaggagcg tatcaactga taacgctccc
    25141 ttattgggcg tgaatatcca acaggttaag attggagaaa aagagcatat cataaaattt
    25201 gacatcaaaa ccacaaatgc aattgaaatg gaacaattaa agcatgattt ggcaggcatt
    25261 ctaaacgttt tagagccagt aaagattact tatggcgatg agccagacaa atactatatg
    25321 gggttgccgg tagatgagat tactccagaa aatttgacaa gatggttcca gcgctcagag
    25381 ttaaaaataa taattcctga tggcgtggct cacagcacga ctttaaaaaa ttttgatatc
    25441 gatacaaatg aaacaagcgc accggatagg atagtattta atttaacaaa tacaggaaca
    25501 gagccagctt atccaattat tagaattaaa cacaactcag agaatggata tattggagtt
    25561 gttaacaaca gagcggcgtt tgagttagga aatcgtgaag aggctgatac tgaaaaatac
    25621 agagactctg aaacattgat agattataga gggactaata ttctaaaagg atttcaaaat
    25681 ggcactaaag gagtagctgt aacaaatgat aataaggagc gtcttgttgg cactttgagt
    25741 acaacaagta tgtgggggcg taatcatatc gaattatcaa accgtggtac agttgaaaaa
    25801 aatcgaaata atgcacaaag tttgacatgg gctattcctg ttgatagtag tggagaagtt
    25861 ggttcattga atgactatct gctttggaga caagttttta tggcagcagt cgctaatcaa
    25921 tatggtttca taaaggttac tgtatcggat acagacggca atttcttgta tggggtggaa
    25981 acttacaaac gctatcagac acttgattgt gagtatagtt ttttcaccac tgacggcaaa
    26041 ggtggataca agtttatcaa atggtggtat tttactggga caggggctca agtaggcaaa
    26101 cttgatccat ttagcgcgga aaagggctgg tcagagttaa aaagaaacga tgatagagtt
    26161 caagtgtttt ttgatggctc tcattatgac tttattattc cggagataaa agacaaaaaa
    26221 tcagcaaaaa tccatatcac gcttggagca ctcagagact ggcctcttat atcacatatg
    26281 tatgttgatg agttcatgta tagaaaagac tttgtgacaa agagtagaga tattcctaat
    26341 cgctatccaa taggttcaaa tgttgtaatc aacagtgagg acgatagtgt gtatattgac
    26401 gggatatcta aagtaagcga agttgtagac ggttcacatt ggccagcaat tcctccagga
    26461 aaatctcaac tagagttgta tttttcacgt tttgttaaga aaaaaccaac tgtaacaatc
    26521 gaatttgaag aaaggtggat ataagatgct tttgacaatc catgatgcaa atttacaaaa
    26581 ggtagcattt attgataacg aaaaacaagg tacgttaaat tattacgatg atacttggac
    26641 aagaagtctt gcaacaggtt cgtcaacgtt tgagtttacg gtatttaaaa aggctgtaaa
    26701 gtctgattta cctcttgcta aagcctatca tcatttgaat gagcatgcat ttgtctcatt
    26761 taagtacaag ggtaaaagct ttgtgtttaa tatcattatt gttgaagaaa atgagcagac
    26821 aatcaaatgt tattgtgaaa atctcaatct tgagttaatc aatgagcttg cgaaccctta
    26881 taaatctaac aaagcgatga ctttcaaaga gtattgtgag gcgatggatc ttttaaatta
    26941 tactcacctt tctattggta tcaatgaaat atcagattat aagcgtactc tggaatggga
    27001 ggggcaagaa accaaactag cccgtctatt aagcctagcc aaacgatttg atgctgagat
    27061 tgaatttgat acacagttaa atgctgatag cactatcaaa aagtttagtg ttaatgttta
    27121 tcatgaaaac gatgacaacc atcaaggggt gggacgtgta agaaatgatg tcattgttaa
    27181 atacggaaaa aacatccact ctattacaag aaaagtggat aagactggta ttttcaatac
    27241 aatcagaccg actggtaaaa tgccaacggt tgaagaagaa ccgagcggag ataagggctc
    27301 caaaagcgaa actgtaaaaa atgcagatgg ttcaacgacg aaaaccacaa tctctacagc
    27361 ctcagatggg actaagagca aaactattgt ccacactaaa gttacaaagt tagcggacaa
    27421 gacacggatc acaacgacca caacgactcg ttctgatggt tccatagaac aaactgttac
    27481 aaccagcaaa aaaggcggag catcaacgtc tgaaacaaaa gtcttgaaaa aaccaaatcc
    27541 aaaagaaaaa acaaatacaa ctgaggatgt tttgacgatt gagggattgg atgaatggga
    27601 agtaaagaac gagaaaggga tagttgaatt ttatcaaaga gggcaagcac tgtatgcgcc
    27661 tatttcaatg caactatatc cctcaacctt tactcattca acaggggagc ttgaccagtg
    27721 gacaagaaaa gattttcatt ttgaaacaga tgagccaaac gagttaagac gtttaggtta
    27781 tctcaaattg aaaaagtatt gttatccagc tatcacttat gaagttgatg gctttgtcga
    27841 tgctgatatt ggagatactg ttaaagtcca tgatgacggt tttgcccctc tattgatgat
    27901 tcaagcacgg gttactgatc aaaaaatcag tttcacaaat ccagtgagaa ataagacaat
    27961 atttgacaat ttcaaggcac ttgaaaacaa actatcagct gatatccagt cagcctttga
    28021 gagattgttt gaagctgcta aaccatatac tatcaaattg tcaacggaca atggtgttat
    28081 ctttaaaaat cagatcggcc agagtctagt aaccccaacc ttatacaagg gaggaaaacc
    28141 agtcgttgtt ggtgttactt ggcgatgggc acttgatgga gaagtaacaa cagggatgac
    28201 ttacttagtt agaggctcaa atgtaactga tacagttact ctgacagttg cagcttacat
    28261 tggaaataaa gaggttgctg ttgatgagat atcgcttgtt aatgttgctg atggaaaact
    28321 tggtacacct ggaactccag ggcgagatgg ccgtactcct tatgtccata cagcatgggc
    28381 taataatgca acaggaacag atggatttag tcttgatagc tcaatcaata aactctatat
    28441 tggtatttat acagactttg aaccaaacga tagcacagac cctaaaaaat acaagtgggc
    28501 taaagtaaaa ggagacaagg gagaaaaagg agataaagga gaaccgggac aacgtggttt
    28561 agatggcttg caaggtgcac gaggtgaaca aggattacct ggtcgcaatg gtgcagatgg
    28621 ccgtactcaa tacactcaca tagcttacag caatagcgct gatggaacta aggatttttc
    28681 tgtaagcgcc tctgatagag cttatatcgg tatgtatgtt gattttaata gtgctgatag
    28741 taatactcca tctgattaca attggacact tgtaaaagga gctgatggcg caaacggcgt
    28801 ggcaggtaag gctggtacag atggtaggac accatactta cacatagctt acgccacatc
    28861 aaataatgga tcacaaggtt tttcaactac tgacagtaca aataaaacgt atatcggaac
    28921 atacacagat tacactcagg cagatagcac agattacaga gtgtataagt ggacgttgat
    28981 aaaaggggca gatggtactg gtatttctaa tgtaactaat tattatttag ctactacagt
    29041 ctcaacaggt atcacaagaa caagcgcagg gtggacaact acgccacagc ctatcacatc
    29101 agacaagcgt tatttatgga attatcgagt tgagctatac acaaacggta caagtaagac
    29161 aacagagcct actgttattg gtgtgcacgg ggataaagga gaacgtggat tacaaggttt
    29221 acaaggcttg caaggcgcac gaggtgaaca aggtattcct ggacctagag gggcagatgg
    29281 tcgtacacaa tatactcaca tggcctatgc cgataacgca acaggtggtg gattcagtca
    29341 aacaaacact gacaaagcct ttgttggggt gtacattgac tttaatccaa cagacagcag
    29401 aaatcctgct gattatcgct ggacaagatg gaaaggtcgt gatggcgcaa atggcgtggc
    29461 aggtaaggct ggtgcagatg gtaggacacc ttatgttcac tttgcgtatt ctgaaaatgc
    29521 agatggatca ggtttgacaa tgacagataa cggacagcgt tattttggtc attattcaga
    29581 ttatgagaaa cctgatagtt cggataaaac taagtacaaa tgggctgatc gttgggctaa
    29641 agttgatgga ggttatgtaa atatctatgc attgtctaag aatagaagta ttgggaaatc
    29701 ttatcatgtt tctgaattta atatggatgt actttcagga aatatcactc taaaagcaat
    29761 tgggtcagat ccatacattg gcgccgtttc ttctcatcca ggtattttta taaagcaaca
    29821 gggaatgaaa atcccagtta tacaagggag atctatttgt ataacaataa caaatccttt
    29881 atttagaaaa aattacatct cgttttttaa ttcattaggt aaaactgtta aaacatataa
    29941 gcattacaac acaaataaat ttttaatatc ttcagctgat ttggtaggtg ttgaatttat
    30001 cgctttacga tatggtgcag gaagctcgaa tatacaaatt ggaaccgtac tagagacaaa
    30061 agtaaaggta gaatatggaa ccgtgcatag cgactggtcg cccgctccag aagatattga
    30121 aagtaatatt aactctaaag ctgatcaggg gctgactcaa gagcagttaa atgccctcaa
    30181 tgagaagtca cagattttag aggctgaaat gaaagcgaaa gcatcgatgg aggcctttag
    30241 tgaattagaa aaagcatata atgcctttgt gaaattaaat gcagatagtc aaaaaaaatc
    30301 tgagtctgat ttagttgaag caggtagaag aattgatttg ctgacgacac aatttggagg
    30361 attagcagag cttaaaacat tcattgatac ttacatgaaa agcacaaatg agggcttgat
    30421 tataggtaag aatgatgcaa gctctactat taaggtatca agtgatagaa tatccatgtt
    30481 ttctgcaggt aaggaagtta tgtacatttc gcaaggtgta ataaatattg ataatggtat
    30541 ttttactgca tcaattcaaa ttggacgttt tagaacagaa cagtatcatc ttaacaaaga
    30601 tgtgaatgtc atacgatata taggaggtta aaaaagaggg aaatgactaa atttatcaat
    30661 tctagcggtt cactacactt gaatatttac atcgaacaag ttagtcaaga tatcgctaac
    30721 aattcctcaa gagttagttg gaaagctact gttgaccgtg atggagctta ccgcacatat
    30781 acttatggta atattagtaa cttgtctgta tggttaaatg ggtcaagtgt gcatagtagt
    30841 catccaaact ttgacacatc cgggcaagag tttactttag caagtgggga agtaaccatc
    30901 ccacacagtg gtgacggaac taagactttt gcagtatggg catcgtttga cccaaacaac
    30961 ggagtacatg gaaacattac cgtgtcagca aactatactc tttcaagcat tcctcgatct
    31021 agtagtgtaa gcgacaatgc tctttcagga aataggcggc tcggaagtcc tcacactctc
    31081 actattgatc gcaaatctag cttatttact caccaagtat ggtatagagt gtttggtagc
    31141 aactggattg atttaggaaa aaatcacgca acaggcgttt ctttcgtacc taatattgac
    31201 ctagctagat acaacacaaa agcaaagtct ggcacgatgg acatatgtgt tcgaacatat
    31261 aatggaacta ctcaagttgg aaatgacgtt tactcaaatg ggtggtattt tgaaattccg
    31321 gaaagcgtga aacctacatt ttctggaatt acattgactg atatgaatac tgttgctagg
    31381 caactattga gcggaaataa ctttttacag attatttccg acattcaggt caactttaat
    31441 aatccatccg gggcttatgg ttcgactatc acaggatatc gtgctgaaat cgtaaacaag
    31501 aatcaggtta caactgtaaa cggtggtagg cttggtatga tgaatttcaa tggttcagca
    31561 acgattcgag ctagtgtggt tgatagccga ggcaggcaat cagatactag agatattaca
    31621 atcaatgtta ttgaatattt tgcaccagct tttagtttta cagcttttag gacgcgggaa
    31681 acacctaaca ttattcaagt tgtcaggaat gctaaaatcg ctcctatcac tttatcaggt
    31741 agtcaaaaaa atgtcatgac actatcattc aaagtagctc gactaggtag tacaactttt
    31801 acagctgatc atggtagagc ttctggtatt tggacaactc aacacacctt aaataattca
    31861 gctgctaaca tggcaggtaa ttatgttgca accaaatcat ttgtggtcat aggaactcta
    31921 tctgataagt ttacaagcac agaatttaca gcaacagttg caactgaaag tgtggtaatg
    31981 agttatgaca aagatggccg tgtgggcatt ggtaaagttg cagagcaagg tggtgcaggg
    32041 tcattggatg tcttgggaga tatctacgct agaaataaac ctattcaaca atatcaattg
    32101 actgacaata atggatgtgg aaaacttatc aaacaggatt ttaatacgat gaaagagact
    32161 ggaacgtggt ggataaatgg tagctctcaa aacaacccct tttctggaac ttgggggatg
    32221 ctagaggtat tcagacctaa tccaggctct catgaacgta ttcaacgctt cactacctca
    32281 acaggatata tggcagttag ggagaatggt tttgataaca actggagacc atggcgctat
    32341 ctggtgcaac aatccaagtc tactaataac tctgactatg tagctttgct aaaatcagaa
    32401 agcactccga ctccttggca aaatgccatt ttacaaaatg gatggaatca tcacagggat
    32461 tacggaggtg tccaattttc aaaaacattc gatggtgttg tttgttttaa aggaacatgt
    32521 aagggcggaa agattgcacg tgagtcaatc atacttactt tacctgaaca tttcagacca
    32581 tctacaacat tattcaaaac tgctttgaat aatgattacg gttcagccgt tataggaatt
    32641 tatccaaatg gaaacgtagt tgtcaaatct aatgtagatg ctacttggct taattttgat
    32701 aatgtgtttt tcaaaatata acaaccgtaa aaaatcccta attattaacg gataattaat
    32761 ttataaagga ggaaatgaca atgttaaaag tcactaaaac acgtcagcta gtaactgaat
    32821 ttttcgcaca agatggcgac caacaaaaat tggtcaaaac tactgtaatc aacacagaca
    32881 ataaagctgt ttcaacaata tctgaaacgc tgcatgaccc ggaactgtac gctaacaatc
    32941 gtatcagtat gcgtaagcat gagcaagagt tacgagaaat gcgctataag attgaagatg
    33001 ccattttggc agagctggaa gcagatgctg agcataagga ataggaggtg tgtatgaaaa
    33061 ttgaattttt caattttcta agaagtgtcg tacagactga agatggtttg gtcttgtacg
    33121 ctctaacact gattgtctca atggaaatca ttgattttgt gacagggacg attgcggcga
    33181 ttatcaatcc tgacatcgag tacaagagca aaatcggcat taacgggctc cttcgtaaga
    33241 tttcaggggt tctcttactg atgatcctca ttccggcgtc cgttttgttg cctgaaaaga
    33301 caggttttgt attcttgcat tcaatctgtc tcgggtacat cgcatttact tttcaatctc
    33361 tcattgaaaa ttaccgcaaa ttaaaaggaa atgttactct ttttcagccg attgtaaaag
    33421 tatttcagcg attacttgaa aaagatgatg atacgaaaaa aggagaataa caaatgcaac
    33481 aaattactga aatcattact aatggagcaa tcagcatcct agtcgttttg gcaggggttg
    33541 tagttagggc agtcaaggac tacctggttc aaaaaggtgg agaaaagacc atcaaaatcg
    33601 ttgaaatctt ggccaagaac gcagtaaatg ccgttgagca ggttgcctca gagactggct
    33661 ttaaaggcga tgaaaagctg gcacaggctc gtgctaaagt ccgtgctgag cttacaaaat
    33721 acaatattag catgactgac aaagacttag acaccttcgt agagtcagca gtgaagcaga
    33781 tgaatgacgc atggaaagga cgatagggaa tggatatcga tagaaacaga ctacgtacag
    33841 gcttgccaca ggttggggtg cagccttatc gacaagtaca tgctcactca acaggtaacc
    33901 gcaactcaac cgtacagaat gaagcggatt atcactggcg gaaagaccca gaattaggtt
    33961 ttttctcgca cgttgttggg aacggtcgtg tcctgcaggt aggacctgtg aacaacggaa
    34021 gttgggatgt tgggggcggt tggaatgctg agacctatgc agcggttgaa ctgattgaaa
    34081 gccattcaac taaggaagag tttatggctg actatcgcct ctatatcgaa ttgctacgca
    34141 atctagcgga cgaagcaggc ttgccgaaga ctcttgatac agacgacttg gcaggtatca
    34201 agacgcatga atactgtacc aataaccaac caaacaacca ctcagaccat gtggatccat
    34261 atccatatct tgctaaatgg ggcattagcc gtgaacagtt taagcaagac atcgaaaacg
    34321 gcttgagagc tgcaacaggc tggcagaaaa atggcactgg ctactggtac gtacactcag
    34381 acggctctta tccaaaagat aagtttgaga aaatcaacgg tacctggtat tatttcgatg
    34441 gctcaggcta tatgctttca gaccgctgga agaagcacac agacggtaat tggtactact
    34501 ttgaccaatc aggcgaaatg gccacaggct ggcagaaaat cgctgacaag tggtactatt
    34561 ttgatgtaga aggtgccatg aagacaggct gggtcaagta caaggacact tggtactact
    34621 tagacgctaa agaaggcgcc atggtatcaa atgcctttat ccagtcagcg gacggaacag
    34681 gctggtacta cctcaaacca gacggaacac tggcagacaa gccagagttc acagtagagc
    34741 cagatggctt gattacagtt aaataaatag aaaggaaact ttctaaattg ttctttcacc
    34801 gcaggctcag gcttgcggtt tttttgtttg ctcaaaaggg gcaaaaaagg ggcaaaagtg
    34861 tcgtaaatct ctgtaaaatg atgtaaaaac atttatttaa aagctcaaaa tatagctgtt
    34921 ttaaaaggta ttgtaagata tagtaaaacg atgtaaaggt atttttaaat gcggatggat
    34981 tgtaatgacg aatgagctga gccacttcaa atcccttttt ctcaattcca tgaatatcga
    35041 tatctaggaa ataaagctga tttacttcat cattttcaat gtattcttca aattcacgga
    35101 cttttcccgt tgtcttgtat gatattggaa tattcgattc tttcgaaatt tcatccaata
    35161 ttctctctag tctcacttga tgttcaataa catcttctaa aattaaaact ttcattcaaa
    35221 ttccctctta aatctaatga tttgtctaaa tgtactgcct tccatctctg tttctaaaat
    35281 aatattgttg tacttatcta gtagttcttt cacattattt aatccgactc cgcgatttct
    35341 tcccttagtg gagaatccta aggcaaatag atctcctgaa ggagtcatcg tcattttaca
    35401 tgaattctga atcacaataa ctgtttcagt ttccatctta ataactgcta cttccatctg
    35461 ctttttatag ctatcagccg atccttcgac agcattattc aataaaacgc tcatgatacg
    35521 aaccaaatcc aatagttcaa ttggaagctt ggtaatcgta tcttttactt ccagtgtaaa
    35581 ctctacacca ttatttcgag catagacaat tgactgagca accaaacttc gtaaagctga
    35641 gtcttctatg ttgttcaaat caaagtaagt gtacttatct gaacgcaatt tatgatttgc
    35701 tttgactaaa acttcattgt aaattctgtc aatttcctgt aaattaccac tgtcaattgc
    35761 catctgcatg ctgacaagca ttccagcata atcatgtcta aaaccacgga tttcattata
    35821 cagaccaaca atttcatctg tgtaattctg taaatgtttc tgttcaaatt tcttctgctt
    35881 caaagcaatc tctttctcca tttgaacttt atgagaattc attgcaaaga aggtcaaaag
    35941 gagagagata aagacaatag atgacaaaat acttccaaaa ctattcaaat gtttaatcgt
    36001 acttaccata tctgaaacga aagatacaat atgtagcaat agtaaagcaa aaaatacttt
    36061 tttcaagaaa ggataaaggt agtccttgtc aaaataggct agttccaaat ggaaataata
    36121 aatgattttt aatgtaacaa aataggttaa caccgtcaca acgaaaaaga atgggaaatg
    36181 atattgtaaa acaaaattat ctcctgttat agaggagaaa gttacggaca gaaagttatg
    36241 agtgctctca tataaaagag atagtagtaa acttaggaat agtcctctat ccctctcata
    36301 ctgtttcatc catcgaaaat aggaatataa gcccaaagga aataaaaatc tttcaatccc
    36361 tattttatct aaatatagaa gataaaagga aaattcaagt actatttcag ttagtaatgt
    36421 ataagcacca aaaacgtata attcttttct atttattcga cctttacaaa ttaaacggta
    36481 actgtgacta ataattaaaa aatgaacaat aactgtccca aatccaagta aatccattac
    36541 tctttctcct tatttcatta cttttttcgt aggaaaagaa aatcaaggat gattcttgaa
    36601 atcctcatct ccccaccttt aatcttttgt aagtcttttt ccttcaaagc tacaaactgt
    36661 tccaatttaa ctgtgttttt cataataaaa tctcctaaaa tgttttttct tgtaagctaa
    36721 cttacaaaaa ccattataca aaatggaatt tcgctttaga taaaattctc tcaactgtca
    36781 tttttttctc ccaaagtgta cttttttaag aaaaaagccg ggaaaattcc cagctttgct
    36841 attatattga tcccagcagg attcgaacct gcgaccgttc gcttagaagg cgaatgctct
    36901 atccagctga gctatgagac ctaacacaat tattctacca aaaattcaat taaaagtcaa
    36961 ttttctattt atggtagggg aatccctgct gaatcgtaaa agcgcgatag atttgttcaa
    37021 caagaactag tctcattaac tgatgaggca aggttaggcg accaaaactg acagaaagat
    37081 tggctctatt ttttacagat gatgacaatc ctaaacttcc cccaataata aaagtaaaag
    37141 tagaaaatcc ttttatagaa gtttcttcta actgcttact aaattcttct gagaagaaag
    37201 ttttcccttc aatggctaac acaataacga aatcacggtc agcaattttt gataaaattc
    37261 tctgaccttc tatttctaaa atcttttgat tttctgattc actggcctta tctggtgttt
    37321 tttcatctga taactcaatc atttcaaact tagcaaatct agaaattcgt tttgaatact
    37381 ctgcgatacc atcttttaaa tacttttctt tcagtttccc aactgttaca actttaattt
    37441 tcatgactct attctaacat attctctatt ttttcacatc ttattcacaa aataaaaaat
    37501 agatttcaat taagaaaatc acagtttcaa aagagttatc cacagtttgt gtaaaacttt
    37561 tgtgtttaag ttataattaa gctagtcagt ttatactttc agtaattcaa acatatggag
    37621 gcaaatatga aacatctaaa aacattttac aaaaaatggt ttcaattatt agtcgttatc
    37681 gtcattagct tttttagtgg agccttgggt agtttttcaa taactcaact aactcaaaaa
    37741 agtagtgtaa acaactctaa caacaatagt actattacac aaactgccta taagaacgaa
    37801 aattcaacaa cacaggctgt taacaaagta aaagatgctg ttgtttctgt tattacttat
    37861 tcggcaaaca gacaaaatag cgtatttggc aatgatgata ctgacacaga ttctcagcga
    37921 atctctagtg aaggatctgg agttatttat aaaaagaatg ataaagaagc ttacatcgtc
    37981 accaacaatc acgttataaa tggcgccagc aaagtagata ttcgattgtc agatgggact
    38041 aaagtacctg gagaaattgt cggagctgac actttctctg atattgctgt cgtcaaaatc
    38101 tcttcagaaa aagtgacaac agtagctgag tttggtgatt ctagtaagtt aactgtagga
    38161 gaaattgcta ttgccatcgg tagcccgtta ggttctgaat atgcaaatac tgtcactcaa
    38221 ggtatcgtat ccagtctcaa tagaaatgta tccttaaaat cggaagatgg acaagctatt
    38281 tctacaaaag ccatccaaac tgatactgct attaacccag gtaactctgg cggcccactg
    38341 atcaatattc aagggcaggt tatcggaatt acctcaagta aaattgctac aaatggagga
    38401 acatctgtag aaggtcttgg tttcgcaatt cctgcaaatg atgctatcaa tattattgaa
    38461 cagttagaaa aaaacggaaa agtgacgcgt ccagctttgg gaatccagat ggttaattta
    38521 tctaatgtga gtacaagcga catcagaaga ctcaatattc caagtaatgt tacatctggt
    38581 gtagttgttc gttcggtaca aagtaatatg cctgccaatg gtcaccttga aaaatacgat
    38641 gtgattacaa aagtagatga caaagagatt gcttcatcaa cagacttaca aagtgctctt
    38701 tacaaccatt ctatcggaga caccattaag ataacctact atcgtaacgg gaaagaagaa
    38761 actacctcta tcaaacttga caagagttca ggtgatttag aatcttaatt gacatctatg
    38821 taaagaaagc tttacataag agaaaagatg tgttagtgta gaatcatgga aaaatttgaa
    38881 atgatttcta tcacagatat acaaaaaaat ccctatcaac cccgaaaaga atttgataga
    38941 gaaaaactag atgaactagc acaatctatc aaagaaaatg gggtcattca accgattatt
    39001 gttcgtcaat ctcctgttat tggttatgaa atccttgcag gagagagacg ctatcgggct
    39061 tcacttttag ctggtctacg gtctatccca gctgttgtta aacagatttc agaccaagag
    39121 atgatggtcc agtccattat tgaaaattta cagagagaaa atttaaaccc aatagaagaa
    39181 gcacgcgcct atgaatctct cgtagagaaa ggattcaccc atgctgaaat tgcagataaa
    39241 atgggcaagt ctcgtccata tatcagcaac tccattcgtt tactttcctt gccagaacaa
    39301 attctctcag aagtagaaaa tggcaaacta tcacaagccc atgcgcgttc cctagttgga
    39361 ttaaataagg aacaacaaga ctatttcttt caacggatta tagaagaaga tatttctgta
    39421 aggaagttag aagctcttct gacagagaaa aaacaaaaga aacagcaaaa aactaatcat
    39481 ttcatacaaa atgaagaaaa acagttaaga aaactactcg gattagatgt agaaattaaa
    39541 ctatctaaaa aagacagtgg aaaaatcatt atttcttttt caaatcaaga agaatatagt
    39601 agaattatca acagcctgaa ataaggctgt tcttttattt ttttatctca caaggttatc
    39661 cactatgttt ttcgataaaa agcttaataa atcaataatt tcttctttta tccccaacct
    39721 gtggataaag tttggtaaca ttgtggatta tttttcacaa cttgtggaaa attcttgcta
    39781 tctatggtaa aatatctcta gtattaaact tttaaatagt aaaggaggag aaaggattga
    39841 aagaaaaaca attttggaat cgtatattag aatttgcaca agaaagactg actcgatcca
    39901 tgtatgattt ctatgctatt caagctgaac tcatcaaggt agaggaaaat gttgccacta
    39961 tatttctacc tcgctctgaa atggaaatgg tctgggaaaa acaactaaaa gatattattg
    40021 tagtagctgg ttttgaaatt tatgacgctg aaataactcc ccactatatt ttcaccaaac
    40081 ctcaagatac gactagctca caagttgaag aagctacaaa tttaactctt tataactata
    40141 gtccaaagtt agtatctatt ccttattcag atacgggatt aaaagaaaaa tatacctttg
    40201 ataactttat tcaaggggat ggaaatgttt gggctgtatc agccgcttta gctgtctctg
    40261 aagatttagc tctgacctat aaccctcttt ttatctatgg aggaccaggc cttggtaaga
    40321 ctcacttatt aaacgctatt ggaaatgaaa ttctaaaaaa tattcctaat gcgcgtgtta
    40381 aatatattcc tgccgaaagc tttattaatg actttcttga tcacctaaga cttggggaaa
    40441 tggaaaagtt taaaaagacc tatcgtagtc ttgatctttt gttaatcgat gatatccagt
    40501 cactcagcgg aaaaaaagtc gcaactcagg aagaattttt caataccttt aacgcccttc
    40561 atgacaagca aaaacagatt gtcctaacga gtgatcgtag tccaaaacat ctagaagggc
    40621 tcgaggagag gcttgtcacg cgttttagtt ggggattgac acaaactatc accccccctg
    40681 actttgaaac acgtattgcc attttacaaa gtaaaacgga acatttaggc tacaatttcc
    40741 aaagtgatac tctagaatac ctagctgggc aatttgattc aaatgttcga gatcttgagg
    40801 gagccatcaa cgacatcact ttaattgcca gagtaaaaaa aatcaaggat atcactattg
    40861 atattgctgc agaagccatt agagcccgca aacaagatgt tagccaaatg ctcgtcatcc
    40921 caattgataa aatccaaact gaagttggta acttttatgg tgttagtatc aaagaaatga
    40981 agggaagtag acgccttcaa aatattgttt tggcccgtca agtagccatg tatttatcta
    41041 gagaactaac agataatagt cttccaaaaa ttgggaagga atttggggga aaagatcata
    41101 ccacagtcat tcatgcccat gccaaaataa aatctttgat tgatcaagac gataatttac
    41161 gtttagaaat tgaatcaatc aaaaagaaaa tcaaataatt tgtggataac ttttactttt
    41221 ttatcttttt tatccacatt ttttaaacaa gctaaaaaac ttgatatgac ttgtttaaag
    41281 gctgttttcc acagatttca cagactctat tattactatt atctttctaa tactaaaaat
    41341 aaataaagga gaatccatga tgcatttttc aattaataaa aatttatttc tacaagcatt
    41401 aaatactact aagagagcta ttagttctaa aaatgccatt cctattttat caacagtaaa
    41461 aattgacgtg accaatgaag gtattacttt aattggttca aatggtcaaa tttcaattga
    41521 aaattttatt tctcaaaaaa atgaagatgc tggtttgtta attacttctt taggttcgat
    41581 ccttcttgaa gcttctttct ttatcaatgt agtatctagt ttacctgatg tgactcttga
    41641 ttttaaagaa attgaacaaa atcaaattgt tttaaccagt ggcaaatcag aaattaccct
    41701 aaaaggaaaa gatagcgaac aatatccacg aatccaagaa atttcagcaa gcactccttt
    41761 aatacttgaa acaaaattac tcaagaaaat tattaatgaa acagcctttg ctgcaagtac
    41821 acaagagagt cgtccgattt taacaggtgt ccacttcgta ttgagtcaac acaaagagtt
    41881 aaaaacagtt gcaacagact ctcatcgcct aagccagaaa aaattgactc ttgaaaaaaa
    41941 tagtgatgat tttgatgtcg taattcctag ccgttctcta cgcgaatttt cagcggtatt
    42001 tacagatgat atcgaaactg tagaaatttt ctttgccaat aaccaaatcc tctttagaag
    42061 cgaaaatatt agcttctata ctcgtctcct agaaggaaac tatcctgata cagatcgctt
    42121 gattccaaca gactttaaca ctactattac ttttaatgtg gtaaacttac gccagtcaat
    42181 ggagcgtgcc cgtcttttat caagtgcgac tcaaaatggt actgtgaaac ttgaaattaa
    42241 ggatggggtt gttagcgccc atgttcactc tccagaagtt ggtaaagtaa acgaagaaat
    42301 cgatactgat caggttactg gtgaagattt gaccattagt ttcaacccaa cttacttgat
    42361 tgattctctt aaagctttaa atagcgaaaa ggtgaccatt agctttatct cagctgttcg
    42421 tccatttact cttgtgccag cagatactga cgaagacttc atgcagctca ttacaccagt
    42481 tcgtacaaat taagtgaaag aggttgagcc tggctcgcct cttttatgat ataatcgaaa
    42541 aagaaaagga gagtagtatg tatcaagttg gaaattttgt tgagatgaaa aaaccacacg
    42601 cttgtacaat caagtcgact ggtaaaaagg ctaatcgttg ggaaattaca cgtgtaggag
    42661 cagatatcaa aataaaatgc agtaattgtg agcatgttgt catgatgggg cgatatgatt
    42721 ttgatcgaaa aatgaataaa attattgact gagaaccctt aattagaggg ttagcacttt
    42781 atcccttttt gtgttagatt gtaatctaca cgcttttaaa cttgtttata gcgcctaaac
    42841 ctttgatatt actgactttt tgaaaaatct catttgatta gaaatcatta aaagttctta
    42901 aaaattctca aaaagtccac aaaaaaagcc ccacaaaagg gcaataaatg acgagttcag
    42961 caggcaagaa actagcacgg tcaaacgtgc tttttttatt gctggttgct gataggtata
    43021 ttataccatg aagcgcgtgg tttataagat tatgacctca caaaagtccc cctgattcgt
    43081 ttctaagggc ttctagttta tccatggtgt tttaatcgtc cgggacgaaa ccaagcgaaa
    43141 tagggaggcg aatatgaagc ctcagcctac atttaccaac atttacgctt ctaattcttg
    43201 tagtaaggtc aaatctaaac catcaaggtc aaaatctatg tttaaatcga tgtctatatc
    43261 catgttaaaa tctatctcca tatcctctaa ttggatatta tccaaatcaa tcaaaaaatc
    43321 ttcaatcatg tcatccccct tcacttaaat ctcaacattt ctcaacaaaa caccacaaat
    43381 gcctacttat acacttctag ctctccgttc tggtggattc tcgcaaaatt taagacggct
    43441 atttgtttat atcggtggta gctagtggag ctagtagctg ccatttctac gcattcgctc
    43501 attgttttct ttctcatgta acaataatgg ataataaagt attttctagc tcgtttgttc
    43561 tctatactgt tgatgtcttg ggcgaatatt tccaacccct cacggattgc tttttcatgc
    43621 ttactagtca aattccactg atcaggtaag acaagtttcc aagcctcttc gtctattgta
    43681 acttggtttt cactccctgc catcctctgg aaacgtagaa agtaggtcat ccgttctctg
    43741 acgttcatca tcgttttaaa cttatccagc tccattagtt cgctcctttg tggtataatt
    43801 agtctaaaga gaagtgaggt agagatgata gtgtcgctac gcaacttcat agtatgagat
    43861 gacgggacag gcacaccgtc cactactctt tagcgcttag attgttctag gcgttttttt
    43921 gtgctcacct attaccccac atttgctaag tgttctatag ctttttgcct tataatgtat
    43981 actgcagtct ttgatttatc tatttcctca gcaacttgcc aagcgtcaag aacattcaag
    44041 tagaacagcc tgagaacaga acgttcaagc ggattggcta gcttatcgat tagcctagat
    44101 atttccattc tctcttcagt aagtcgctca attctctgta aagtgtactc ttttagtttt
    44161 agaacattta taaggttatt ctcggtcgta tttaccctgc ttgtttgtac tctggtattg
    44221 cttagttctt gctttttgat aataccactt tctaaagctg caagctctaa atataagcct
    44281 tgtttgatag tcgggtataa gatataagag tagccaaaca aagcgctaga aacggcttct
    44341 atgaaatcct cagtcgtccc ctgtatatcg tcataatcaa ggaaaatcaa atcccgatag
    44401 attagactgg tgttgtttcg cttgtaactg ccattctttt ctggtgtcac tttgccacta
    44461 atacagtaag gcgcttgtat acgtttaaat tcttcaatgt ttgccccttc agggactacc
    44521 aaaggcttaa agcgttcaat gtactggaac ggctccatct tatcaaatgg atagacaaga
    44581 ttactctgga aacctctagc ctcataaatt gtcatactat cgcccctttt tgcgtttttt
    44641 ttaacttctt cagtagcttt tgttcttgga actgttccaa agatctgcca cggtgcttat
    44701 acaatttaat gtcatgataa tgacctccgc cttgcgctgg gtgtgtgtta tatctaccca
    44761 ttctccaccc ccataaacct acgaatgtca tccactaaat aaaatattat tctagcacca
    44821 tcgtccggtg gttgatagcg tcttaaaccc tgactttccc acttattcag cgtgttgtca
    44881 ctaattttta actcttcttt gagttcaact ctatttatta acttggtcaa tcttggcggt
    44941 actttctcac gcgcttccag gtatctttcc actacctcca gaatgccatg cgtcaaatca
    45001 tgctcacttt ctcggcttaa gctaaacata tccgcccacc tccttcaagg tttctttgta
    45061 gctttctagg tcgctattca tcaacaccgc taggcgcttg ctctcctcct gtacttggtt
    45121 gtaaaaagcc ttagcgccat ctagtaactc gcctttattc gctggaataa agtacccact
    45181 aaaaacgcca catctaaccc ctacaatagg gatgttgtgc ttaactacta gacgcttgat
    45241 aatgtcacgt acagttcttt cagttagctt ggttgtcagg ctaatttctg accctgttat
    45301 ggagttctca gcccctacct tgattagtct taaaactcgc ttgtcattct ctgatagact
    45361 catccaaccc ctcctctcct ttgcttttat ttacaaaggt tatttgtccg ctcgctaccc
    45421 cttgcaaaat atctttttga gtttctatga ttgtatccaa ggttttcaaa atagctgtct
    45481 cagtctctaa atccaacgtt tgttctgtca acaagctcat agttacagct aactttgttg
    45541 cattgtccat acacgtagct agaaaaatat ctttagtaaa ataaggttca tctggtattt
    45601 tttcaatatc ggggtataaa ttcattgtgt aactcattcc atccctccgg aaactcttac
    45661 ccctgcaagc tggatatatc gcccataatc agggtttaaa tcctcgctag gtgtttttat
    45721 cgtctgttgg ttttctcgct caatttgggc gctttttttg cggtctcggt ggtttaaata
    45781 aagcagtaag ccaatcaaaa tcacgattaa gacagcagca tgtgtgttac ttatatctag
    45841 ttcattcatg ttatgccctc gcttgataat tcttaatata ttccacttgt tcagctcgct
    45901 ccatattcaa caagtcgtcc acctcttcgg gtgttacctt tctatctaaa aaatcagtga
    45961 tgagctgtaa taggtttgga tttcttgcct tgatgtcagc cattacttca tcaaattctg
    46021 cttgtgtcat tttgcctagg tctagtgtca ctgcattgcc tcctaatcaa tcatcattcc
    46081 acgagtatat aaggatgaat ctgtccatga atccaaaacg ttcttttttc ctcgtttttt
    46141 ctttgcgata tcaaccgcta tgacttccca tacataattc aataattgac tttgttccat
    46201 tgggtaggta ttttctttag ttatttcagc taggtaagtg tttaagaaaa gaaatccatc
    46261 aaagttggaa agtagcctta taaactgagt atcaccccaa aaatttctgt aataagactc
    46321 ttctcgttct tctagttcac ctattacctc agcaatcccc ctctgtttca tcttttctgt
    46381 ttgttccttt atcgtttgct gttgttcatg gtattctgtg ctagttagtt cactgtaaac
    46441 atcatccaag tcgttcaaca ctctactgat ttctctttta gctaatacag tttcagcccc
    46501 tacaaggctt ttttgtattt cttctaaatt tgttgaaata gcttctaatt tatctgatat
    46561 catgttttcc tccattggcc ttttaattgt cctgataaaa cccatattct agaaaatcgt
    46621 tattttcgtc agcttggtgt aattccatac taattctatc caagctttta gcgataatat
    46681 cgtatgttgt ttcttccata cttgtacctc tgtttctatg ttgtgtaatt gccctgatgg
    46741 cttcttaatg cttttttctt gtaattgccc tgatggcttc ttaatgcttt tttcttgtaa
    46801 ttgctatcct cacgctctcc ttggccaaaa tttgagtgtg gggattttga atggttgttt
    46861 cttatacagt ttttcctgtc acatgcctca cgctcagact cgccaaattg aaagcgtgag
    46921 aaagtaccag tttaaagagt tggcgctcta tgcttttcaa aaccttttct aattgcttgc
    46981 ctgctcttcg gttttcttag tttttcttcg tttcgttttc gatttgatcg cctaatcttt
    47041 tccaggctct atcaaattca tcacgttgaa tttctttact atgtaattgc cttgctaact
    47101 ccatgcctct acgcatgaat ctagcgaagt gggtttttgc ttccatcctc cccctccatc
    47161 atgtcataaa gtagggcgta atgtttatct gggattctgt ccaaggcttt caagccgtcg
    47221 tgttctgctt tttgtctggt ttccgccttg accgtactat caaaggcgat agaaaaagca
    47281 ttcagcattg ccttgtagcg gtctacactc ttcaaatagc gcccacgttc tgacaattct
    47341 ttatcttcca attcctcttt taccgtaccg tctaatagtg cgaacttgct ataaactcct
    47401 ttttctagtg taaaatctag gcgattgttt tgtttcaact tgcagaggtt ggttctgcaa
    47461 ctctctaggt tacggtaacc taatacttta gtaatttctt ctaggcgctt gccctctaat
    47521 tcaggcaatt tttcagcaat atctttaaat tttactactt gatgtttctt tctcattgta
    47581 tacctcattt ttgggtacgc aaaaagcgta ctctatttct atgtttgaca aataagagta
    47641 cgcatgataa gatatttacg tacctcattt gtggtgcatc gccgtctatc gtgttctgtc
    47701 gccaaacaag tgacacggta ggcggtttcg ttatggttca gactttacaa gctcaatccc
    47761 tcgcatgact atatctgttt tagtcatgtt tttctttttg gcaagttctt ctaaattatt
    47821 aaaatcctgt tcagtcattt taatttttag ttgcttgttt tttgggttat cgcttttagg
    47881 tcgtcctaat gttttcccca tttttgacct ctctttcttt cggggtaccc ttttattatg
    47941 taacggtgta ccctttaagt caacccctaa accgaaaaaa atttaaaatc ttcccagcgt
    48001 accttatttt attttcaatg tactagcgga aaccgctata ttatgctata atctaggtat
    48061 agaaaaaata tctataccct aaatcttgtc gcttgcctcg gtcgccaaac tatcagcaag
    48121 tgactttttt tattgtcttt ttcttgcttt cttccctgtc tgggggctat aaagcaaatc
    48181 tttactttcg ataagatcca gaatccagct gaatccctgc tccaccgttt caagaaatgc
    48241 gcctaggtct tcattgtcca agttctcata gttcatacaa agatattcgg ctagttgtct
    48301 gtccatctca actagctttt taaaatcctt gggatactta ggaatttcta accctttggc
    48361 atttgtaact gtcttaaatt cattttccat tttctatact cctatgcttt aaaaattagt
    48421 tcttcaattt ctgaatacac cctattcaag ttaatcatcg ctattgccat atcttccaaa
    48481 cgttggtagt ttgtcagttc cgcgcttgtt aaactgtcaa taccgttttc actttctcgc
    48541 tcttgcatga gttgggcttt gtttttccca gtcgctccct ttagcagtag gtttgtaagt
    48601 gtactatagg catgcttggg cgctttctcc catgatttga tagcttcggt taaggtcttg
    48661 cgctttggct tttccagttc tctttgaaga tagcgtttag aaagctcctc acgcatttca
    48721 aagaatgctt tgactaggtt cattttgaac cgccgtacag gttcagtatt ctttaaataa
    48781 gtgatcagca aggtcgcttg ttgttcattt aggagatata ttttcattgg tctccctcgg
    48841 ccgtctaatt atcgcatttc aaatgcgata attccgtagc tttcaaaatc agctttatgc
    48901 ttccttaaaa gttcttgtat tgtgtgatga gtgactccag cacattcagc gatgatctca
    48961 ctcgtagtat acggctcttt ctttccgtcc atgtagacta gttccattgg tttgctcctt
    49021 tctagctata aaataattca tctattgtta tgtctggttt gatttctgcc accattgact
    49081 taaaataata ctctcattat tgcacctctc ttttatttag ttctattttt ttcgtactgt
    49141 ctcctaaaaa aatattgtcc aatttaactt ggtaaattgc agacaattct tttaaaagtt
    49201 caaacggtat atatgtacta tctttttcgt acttggagac agtttgaaaa ttcttattta
    49261 cctttttagc aatctgtcgc aaagtataac cagcgtttac tctacaggct ttcaacgtcc
    49321 attgttccat ataatctaac aattcctttc aaaagtgata agttaatagt actattattt
    49381 tcgtactttg tttttatttt gtgttataat caatgtataa cttacaactg aaagggttaa
    49441 agtcatggct aaaaatagtc ctcaagatct tgaaaataga gaatacttct ctaaacggct
    49501 caaccttctt atggaacaaa aaggggttag acaaattgac cttcataacg ctttagatat
    49561 tcctaaaagt acaattacgg gatatgtaaa aggaaaatct ttaccaaccg ctgggaattt
    49621 acaaaaaata gctgattttt tgaacgtaaa aaaatctttc ctagatctgc ggtttattga
    49681 tacagtttca accagcacac taaaagagtt gctagataat aatcaaagag aaaaggtttt
    49741 tgagatgttc ttcaataaaa tcaaagaaat acttagcttt aaaaaagatt atatgaatat
    49801 ggagtacatt cgaggaaaaa tagcatttga ccagggtgtt ttgggtcgcg ttcttcagtc
    49861 ttcaataata aaccctggta ttgttgaaat tttcaacgga cttgatttat cagatatctc
    49921 aaaaaataat gaggctctac tactttccaa acagcgcgaa gctagggagt acgcttataa
    49981 taatgtactc accatgatta ctaacgaata tattacacgt ttaataaacg ttattgaacg
    50041 tgagcaagct ctcaatacct cctatgtgca ttctgaaatt tctaatctta tcaatagcct
    50101 taacaataaa attgatagct atggggcagt tgtctttgaa gtgaatgaaa taattgaaaa
    50161 aataggtatt caagatcgaa aaatcttagg ctattgctta caaaggaaaa tatcttctgt
    50221 tcaaaatgag ataaatgacc ttgttcacaa taatacaaat aatagctttt gtgttacaaa
    50281 aaacaagata tttactgaaa aaagtgacat cattgacacc attgaatttg attactataa
    50341 aggtattcag aaccgcttga atgatttagc tgaagaaatc agggaacact tcagcgacta
    50401 acccacgcgc cacaatgttc aacaatcctg aagcgcgtgt attcgcggag ttcaagattt
    50461 tctaatatac tctaacgaca cgaaaataaa ataaaactct tgaaatatcg ggagttattg
    50521 gcaaattaac tcgacaaaaa ggagaaatta tgactgatct gacaacctca caaatagaaa
    50581 gacaaaatat tttaaataat tctttagcat tacaaaaagc tgaagagatt ttaaaagtac
    50641 ctggtttcta ctttgaggat actttttatt ttacaaattc tcagctagct acctttttcg
    50701 aagtcgacat tcgaactatt gaaagacttg ttgaagctca taaaacggag cttactgaaa
    50761 atggttatca cactttacgt ggagaaaaac tagctaaatt taaagaaaat gcttttgcta
    50821 ccgacacaaa tgtcggtagc aaagttacac aattaagtat ctcatcgttc agaacattgc
    50881 ttaattttgc tatgttgtta accaatagtg atatagctaa gcaagtaaga aataccctac
    50941 ttgatattgt cattaatgtg ttaactgaga agactggtgg ccatgtaaaa tttataaacc
    51001 agcgtgacaa aaattatcta tcgactgcat tagaagaaga taatgctaga aaaatattta
    51061 caaatgccat tgataagtat gtctctggaa gtaattttaa atatggtcaa ttaacgaatg
    51121 aaatttacaa agcaatcttt aaagaaagag catcagaata taaaaaaata ctgaaattga
    51181 ataaaaatga taatgctcgc gcaacaatgt atagtgaggt acttatagtt attgctagtt
    51241 ttgaaaaagg ggttgcttat gagattgaac aaaagagtaa gtcttcaaat caaaaactaa
    51301 ctaatactga tgtaatcgag ataattcaga gattagcaaa tcacccaatg caagaacccc
    51361 atttaatgca tgccagagag aaaatggcta gtagggatct atctttcagg aatgccaaac
    51421 atgagaaatt agcagaatat atccgtccaa tttctgaaga agattttgaa agatttattg
    51481 gcgaacaaag taaatcactt tcggaacaaa ttgatgaaca ccgccaagtt tttgaaagat
    51541 tgagagataa ataatggata aaatcaatta tttttctgct gaagaagcaa ttttactaca
    51601 cgacattgaa gtaattgaga aaataggtgg tttacacggt gttaaagata agggaagggt
    51661 atttagtgta tttgaacact tacagaatga cgattactat ccagactttg aaacaaaact
    51721 tacagtgcta gtatttaata cagttcaatt tcatatgttc aatgatggaa ataagagaag
    51781 tagcattgtc ttcggtatta aatttttaac aatcaatcac cttgattacc tcattccctt
    51841 ctttatcgaa acaatggaaa atattgtctt atgggtagca atggggttaa tcgatttcaa
    51901 ttttttaaaa gaaattattt cttcaattct ctactatgag gaactcactg aaaaagtaaa
    51961 agaaaaactt gtacagatag ctttagctga agaaaataac aaataaaatc tttcccgtaa
    52021 gctcaacatg atataaacca taatctaaaa cctttttaat aatagcttgc ctgctgatgt
    52081 attttagaaa ggtttatcat catgaaaata acagaataca ccaaaaaaga cgggtctaca
    52141 gtctatcgct ctagcgtcta ccttggtatc gataccgtaa caggtaagaa agtcaagacg
    52201 actatatcag ggcgaaccaa gagagaactc aaagccaaag cgctacaggc ttaaatcgac
    52261 tttgaaaaag acgggtctac agtatataaa gcagttgaga taaagactta cgccgaactg
    52321 gtggagaatt ggctagaaac ctactgccat acggttaaaa aatcgacctt aatgggtacc
    52381 aaattcaaaa tagataaata tctacttcca gctttcggaa attataggct ggataaactg
    52441 acgcccccaa tcattcaaaa gcaagtcaat caatgggcaa aagactataa tcaactaggc
    52501 aaagggtttc aagagtatcc tctccttcac tcgctaaaca aacgcatact taaatatgcc
    52561 gtatctttgc aagctatccc cttcaaccct gcccgtgata ttatcgtgcc acgtcgtaag
    52621 gaaaaaaaag ggcaaaaact gaaatatctg gatgatgata acttaaaaaa attcttgact
    52681 tacctggagc agctgccaaa cacttacaaa aatttctacg atacggtgct atataagacg
    52741 cttttagcga ctggtttgcg cattcgtgag tgtctagcct tagagtggtc tgatattgac
    52801 ctaaaaaacg gcacactaga cgtaaataaa acgttaaata ttatcaaaga aatcactggc
    52861 cctaagacta aatcaagtat tagggtaatt gacttggata ataagacggt gctcatgcta
    52921 cggctctata aagcaagaca atcccaaata gggaaagaaa tggggttgac ctatgagaaa
    52981 gtattctcta atagctttga taagcatata gacgctagaa tgcttagttt caggttagta
    53041 aaacatttgg aacgggctgg atgccctcgt tttacgttcc acgccttccg ccacactcat
    53101 gctagtatct tgctcaatgc tggactacct tacaaagaga tacaaacacg gctcggtcat
    53161 gcaaaacttt ctatgaccat ggatatttat agccacctat ccaaagacaa tcagaaaaat
    53221 gctacttcat tttatgaaaa agctattgaa aaattaaaaa gttcctaaaa aagtccataa
    53281 aattattatt tcagttatgg gaaatagcta tatcaaggaa ttttagcaag tttccccttt
    53341 ttgtgttata atattaggga ttgaaatgaa aacggagaat gagaaatatg gctttgacag
    53401 caggtatcgt tggtttgcca aacgttggta aatcaacact atttaatgca attacaaaag
    53461 caggagcaga ggcagcaaac tacccatttg cgacgattga tccaaatgtt ggaatggtgg
    53521 aagttccaga tgaacgccta caaaaactaa ctgaaatgat aactcctaaa aagacagttc
    53581 ccacaacatt tgaatttaca gatattgcag ggattgtaaa aggagcttca aaaggagaag
    53641 ggctagggaa taaattcttg gccaatattc gtgaagtaga tgcgattgtt cacgtagttc
    53701 gtgcttttga tgatgaaaat gtaatgcgcg agcaaggacg tgaagacgcc tttgtggatc
    53761 cacttgcaga tattgacacc attaacctag aattgattct tgcagactta gaatcagtca
    53821 ataaacgata tgcgcgtgta gaaaagatgg cacgtacgca aaaagataaa gaatcagtag
    53881 cagaattcaa tgttcttcaa aagattaaac cagtcctaga agacgggaaa tcagctcgta
    53941 ccattgaatt tacagatgag gaacaaaagg ttgtcaaagg tcttttcctt ttgacgacta
    54001 aaccagttct ttatgtagct aatgtggacg aggatgtggt ttcagaacct gactctatcg
    54061 actatgtcaa acaaattcgt gaatttgcag cgacagaaaa tgctgaagta gtcgttattt
    54121 ctgcgcgtgc tgaggaagaa atttctgaat tggatgatga agataaaaaa gagtttcttg
    54181 aagccattgg tttgatagaa tcaggtgtag ataagttgac gcgtgcagct taccacttgc
    54241 ttggattggg aacttacttc acagctggtg aaaaagaagt tcgcgcttgg actttcaaac
    54301 gtggtatgaa ggctcctcaa gcagctggta ttatccactc agactttgaa aaaggcttta
    54361 ttcgtgcagt aaccatgtca tatgaggatc tagtgaaata cgaatctgaa aaggccgtaa
    54421 aagaagctgg acgcttgcgt gaagaaggaa aagaatatat cgttcaagat ggcgatatca
    54481 tggaattccg ctttaatgtc taaaaattaa taaatggtgt caattaggtt ggaaaaaaat
    54541 tccaaccctt ttggcttttg aaaggaaaaa taaatgacca aattacttgt aggcttggga
    54601 aatccagggg ataaatattt tgaaacaaaa cacaatgttg gttttatgtt gattgatcaa
    54661 ctagcgaaga aacagaatgt cacttttaca cacgataaga tatttcaagc tgacctagca
    54721 tcctttttcc taaatggaga aaaaatttat ctggttaaac caacgacctt tatgaatgaa
    54781 agtggaaaag cagttcatgc tttattaact tactatggtt tggatattga cgatttactt
    54841 atcatttacg atgatcttga catggaagtt gggaaaattc gtttaagagc aaaaggctca
    54901 gcaggtggtc ataatggtat caagtctatt attcaacata taggaactca ggtctttaac
    54961 cgtgttaaga ttggaattgg aagacctaaa aatggtatgt cagttgttca tcatgttttg
    55021 agtaagtttg acagggatga ttatatcggt attttacagt ctgttgacaa agttgacgat
    55081 tctgtaaact actatttaca agagaaaaat tttgagaaaa caatgcagag gtataacgga
    55141 taaatggtga ccttattaga tttattctca gaaaatgatc agattaaaaa atggcatcaa
    55201 aatttaacag ataagaaaag acaactaata cttggtttat caacatctac taaggctctt
    55261 gcaattgcaa gcagtttaga aaaagaagat aggattgtgt tattgacgtc aacttatgga
    55321 gaagcagaag gacttgttag tgatcttata tctatcttgg gtgaggaact cgtctatcca
    55381 tttttggtag atgatgctcc tatggtggag tttttgatgt cttcacagga aaaaattatt
    55441 tcacgggttg aagccttgcg ttttttgact gattcatcta agaaagggat tttagtttgt
    55501 aatatcgcag caagtcgatt gattttaccg tctcccaatg cattcaaaga tagtattgta
    55561 aaaatctcag ttggtgaaga atatgatcaa cacgcgttta tccatcagtt aaaggaaaat
    55621 ggctatcgaa aagttactca agtacaaact cagggcgaat ttagtcttcg aggagatatt
    55681 ttagatattt ttgaaatatc ccagttagaa ccttgtcgaa ttgagttttt tggtgatgaa
    55741 attgatggta tcaggtcatt tgaagtagaa acacaattat cgaaagaaaa taagacagaa
    55801 ctcactatct ttccagctag tgatatgctt ttgagagaaa aggattatca acgaggacag
    55861 tcagctttag aaaaacaaat ttcaaaaact ttatcaccta ttttgaaatc atacctggaa
    55921 gaaattcttt caagttttca ccaaaaacaa agtcatgcag actctcggaa gtttttatct
    55981 ttgtgctatg ataagacatg gactgtcttt gattatattg aaaaagatac tccaatattc
    56041 tttgatgatt atcaaaaatt gatgaatcag tatgaagtct ttgaaagaga cttagcgcag
    56101 tactttacag aagaattaca gaatagtaaa gcattttctg atatgcagta tttttctgat
    56161 attgaacaaa tctataaaaa acaaagtcca gtgacctttt tctctaatct tcaaaagggt
    56221 ttaggaaatc tcaaatttga caaaatttat caattcaatc aatatcctat gcaggaattt
    56281 ttcaatcagt tttcttttct aaaagaagaa attgaacgat ataaaaaaat ggattacacc
    56341 attattctgc agtctagcaa ttcaatggga agtaaaacat tggaggatat gttagaggaa
    56401 tatcagatta aattggattc tagagataag acaagtatct gtaaagaatc tgtaaactta
    56461 atagagggta atctcagaca tggttttcat tttgtagatg aaaagatttt attgataact
    56521 gaacatgaga tttttcaaaa gaaattaaag cgtcgttttc gaagacaaca tgtttcaaat
    56581 gcagagagat taaaagatta caatgaactt gaaaaagggg actatgttgt ccatcatatc
    56641 catgggattg gtcaatatct aggaattgaa accattgaaa tcaagggaat tcatcgcgat
    56701 tatgtcagtg tccaatacca aaatggtgat caaatttcta tccccgtgga acagattcat
    56761 ctactgtcca aatatatttc aagtgatggt aaagctccaa aactcaataa attaaatgac
    56821 ggtcatttta aaaaggccaa gcaaaaggtt aagaaccagg tagaggatat agctgatgat
    56881 ttaatcaaac tctactctga acgtagtcag ttgaagggtt ttgctttctc agctgatgat
    56941 gatgatcaag atgcctttga tgatgctttc ccttatgttg aaacggatga tcaacttcgt
    57001 agtattgagg aaatcaagag ggatatgcag gcttctcagc caatggatcg acttttagtt
    57061 ggggatgttg gttttggaaa gactgaagtt gctatgcgtg cagcctttaa agcagtcaat
    57121 gatcacaaac aggttgtcat tctagttccg acgacggttt tagcgcaaca gcactatacg
    57181 aattttaagg aacgattcca aaattttgca gttaatgttg atgtgttgag tcgctttaga
    57241 agtaaaaaag agcagactgc aacacttgaa aaattgaaaa acggtcaagt cgatattttg
    57301 attggaacac atcgtgtttt gtcaaaagat gttgtgtttg ctgatttggg cttgatgatt
    57361 attgatgagg aacagcgatt tggtgtcaag cataaggaaa ctttgaaaga actgaagaaa
    57421 caagtggatg tcctaacctt gaccgctacg ccaatccctc gtaccctcca tatgtctatg
    57481 ctgggaatca gagatttatc tgttattgaa actccgccga ctaatcgcta tcctgttcag
    57541 acctatgttt tggaaaagaa tgatagtgtc attcgtgatg ctgtcttgcg tgaaatggag
    57601 cgtggaggtc aagtttacta tctttacaac aaagttgaca caattgttca gaaggtttca
    57661 gaattacagg agttgattcc ggaggcttcg attggatatg ttcatggtcg aatgagtgaa
    57721 gtccagttgg aaaatactct attagacttt attgagggac aatacgatat cttggtgacg
    57781 actactatta ttgagacagg ggtggacatt ccaaatgcta atactttatt tattgaaaat
    57841 gcggaccata tgggcttgtc aaccttatat cagttaagag gaagagtcgg tcgtagtaat
    57901 cgtattgctt atgcttatct catgtatcgt ccagaaaaat caatcagtga agtctctgaa
    57961 aagagattag aagcgattaa aggatttaca gaattgggct ctggctttaa gattgcaatg
    58021 cgagatcttt cgattcgtgg agcaggaaat cttttaggaa aatcccagtc tggtttcatt
    58081 gattctgttg gttttgaatt gtattcgcag ttattagagg aagctattgc taaacgaaac
    58141 ggtaatgcta acgctaacac taacacaaga accaaaggga atgctgagtt gattttgcta
    58201 attgatgcct atcttcctga tacttatatt tctgatcaac gacataagat tgaaatttac
    58261 aagaaaattc gtcaaattga caaccgtgtc aattatgaag agttacaaga ggagttgata
    58321 gaccgttttg gagaataccc agatgtagta gcctatcttt tagagattgg tttggtcaaa
    58381 tcatacttgg acaaggtctt tgttcaacgt gtggaaagaa aagataataa aattacaatt
    58441 caatttgaaa aagtcactca acgattgttt ttagctcaag attattttaa agctttatcc
    58501 gtaacgaact taaaagcagg catcgctgag aataagggat taatggagct tgtatttgat
    58561 gtccaaaata agaaagatta tgaaatttta gaaggtctgc tgatttttgg agaaagttta
    58621 ttagagataa aagagtctaa ggaaaaaaat tccatttgat atttttcttc tataaaaaag
    58681 ataaaaatgg tacaataata aattgaggta ataaggatga gattagataa atatttaaaa
    58741 gtatcgcgaa ttatcaagcg tcgtacagtc gcaaaggaag tagcagataa aggtagaatc
    58801 aaggttaatg gaatcttggc caaaagttca acggacttga aagttaatga ccaagttgaa
    58861 attcgctttg gcaataagtt gctgcttgta aaagtactag agatgaaaga tagtacaaaa
    58921 aaagaagatg cagcaggaat gtatgaaatt atcagtgaaa cacgggtaga agaaaatgtc
    58981 taaaaatatt gtacaattga ataattcttt tattcaaaat gaataccaac gtcgtcgcta
    59041 cctgatgaaa gaacgacaaa aacggaatcg ttttatggga ggggtattga ttttgattat
    59101 gctattattt atcttgccaa cttttaattt agcgcagagt tatcagcaat tactccaaag
    59161 acgtcagcaa ttagcagact tgcaaactca gtatcaaact ttgagtgatg aaaaggataa
    59221 ggagacagca tttgctacca agttgaaaga tgaagattat gctgctaaat atacacgagc
    59281 gaagtactat tattctaagt cgagggaaaa agtttatacg attcctgact tgcttcaaag
    59341 gtgataaaat ggaaaattta ttagacgtaa tagagcaatt tttgagtttg tcagatgaaa
    59401 agctggaaga attggctgat aaaaatcaat tattgcgttt acaagaagaa aaggaaagga
    59461 agaatgcgta aattcttaat tattttgttg ctaccaagtt ttttgaccat ttcaaaagtc
    59521 gttagcacag aaaaagaagt cgtctatact tcgaaagaaa tttattacct tttacaatct
    59581 gactttggta tttattttag agaaaaatta agttctccca tggtttatgg agaggttcct
    59641 gtttatgcga atgaagattt agtagtggaa tctgggaaat tgactcccaa aacaagtttt
    59701 caaataaccg agtggcgctt aaataaacaa ggaattccag tatttaagct atcaaatcat
    59761 caatttatag ctgcggacaa acgattttta tatgatcaat cagaggtaac tccaacaata
    59821 aaaaaagcat ggttagaatc tgactttaaa ctgtacaata gtccttatga tttaaaagaa
    59881 gtgaaatcat ccttatcagc ttattcgcaa gtatcaatcg acaagaccat gtttgtagaa
    59941 ggaagagaat ttctacatat tgatcaggct ggatgggtag ctaaagaatc aacttctgaa
    60001 gaagataatc ggatgagtaa agttcaagaa atgttatctg aaaaatatca gaaagattct
    60061 ttctctattt atgttaagca actgactact ggaaaagaag ctggtatcaa tcaagatgaa
    60121 aagatgtatg cagccagcgt tttgaaactc tcctatctct attatacgca agaaaaaata
    60181 aatgagggtc tttatcagtt agatacgact gtaaaatacg tatctgcagt caatgatttt
    60241 ccaggttctt ataaaccaga gggaagtggt agtcttccta aaaaagaaga taataaagaa
    60301 tattctttaa aggatttaat tacgaaagta tcaaaagaat ctgataatgt agctcataat
    60361 ctattgggat attacatttc aaaccaatct gatgccacat tcaaatccaa gatgtctgcc
    60421 attatgggag atgattggga tccaaaagaa aaattgattt cttctaagat ggccgggaag
    60481 tttatggaag ctatttataa tcaaaatgga tttgtgctag agtctttgac taaaacagat
    60541 tttgatagtc agcgaattgc caaaggtgtt tctgttaaag tagctcataa aattggagat
    60601 gcggatgaat ttaagcatga tacgggtgtt gtctatgcag attctccatt tattctttct
    60661 attttcacta agaattctga ttatgatacg atttctaaga tagccaagga tgtttatgag
    60721 gttctaaaat gagggaacca gattttttaa atcattttct caagaaggga tatttcaaaa
    60781 agcatgctaa ggcggttcta gctctttctg gtggattaga ttccatgttt ctatttaagg
    60841 tattgtctac ttatcaaaaa gagttagaga ttgaattgat tctagctcat gtgaatcata
    60901 agcagagaat tgaatcagat tgggaagaaa aggaattaag gaagttggct gctgaagcag
    60961 agcttcctat ttatatcagc aatttttcag gagaattttc agaagcgcgt gcacgaaatt
    61021 ttcgttatga tttttttcaa gaggtcatga aaaagacagg tgcgacagct ttagtcactg
    61081 cccaccatgc tgatgatcag gtggaaacga ttttgatgcg cttgattcga ggaactcgct
    61141 tgcgctatct atcaggaatt aaggagaagc aagtagtcgg agagatagaa atcattcgtc
    61201 ccttcttgca ttttcagaaa aaagactttc catcaatttt tcactttgaa gatacatcaa
    61261 atcaggagaa tcattatttt cgaaatcgta ttcgaaattc ttacttacca gaattggaaa
    61321 aagaaaatcc tcgatttagg gatgcaatct taggcatcgg caatgaaatt ttagattatg
    61381 atttggcaat agctgaatta tctaacaata ttaatgtgga aaatttacag cagttatttt
    61441 cttactctga gtctacacaa agagttttac ttcaaactta tctgaatcgt tttccagatt
    61501 tgaatcttac aaaagctcag tttgctgaag ttcagcagat tttaaaatct aaaagccagt
    61561 atcgtcatcc gattaaaaat ggctatgaat tgataaaaga gtaccaacag tttcagattt
    61621 gtaaaatcag tccgcaggct gatgaagagg aagatgaact tgtgttacac tatcaaaatc
    61681 aggtagctta tcaaggatat ttattttcct ttggacttcc attagaaggt gaatcaattc
    61741 aacaaatacc tgtttcacgt gaaacatcca tacacattcg tcatcgaaaa acaggagatg
    61801 ttttgattca aaatgggcat agaaaaaaac tcagacgttt atttattgat ttgaaaatcc
    61861 ctatggaaaa gagaaactct gctcttatta ttgagcaatt tggtgaaatt gtctcaattt
    61921 tgggaattgc gaccaataat ttgagtaaaa aaacgaaaaa tgatataatg aacactgtac
    61981 tttatataga aaaaatagat aggtaaaaaa tgttagaaaa cgatattaaa aaagtcctcg
    62041 tttcacacga tgaaattaca gaagcagcta aaaaactagg tgctcaatta actaaagact
    62101 atgcaggaaa aaatccaatc ttagttggga ttttaaaagg atctattcct tttatggctg
    62161 aattggtcaa acatattgat acacatattg aaatggactt catgatggtt tctagctacc
    62221 atggtggaac agcaagtagt ggtgttatca atattaaaca agatgtgact caagatatca
    62281 aaggaagaca tgttctattt gtagaagata tcattgatac aggtcaaact ttgaagaatt
    62341 tgcgagatat gtttaaagaa agagaagcag cttctgttaa aattgcaacc ttgttggata
    62401 aaccagaagg acgtgttgta gaaattgagg cagactatac ctgctttact atcccaaatg
    62461 agtttgtagt aggttatggt ttagactaca aagaaaatta tcgtaatctt ccttatattg
    62521 gagtattgaa agaggaagtg tattcaaatt agaaagaata atctttaatg aaaaaacaaa
    62581 ataatggttt aattaaaaat ccttttctat ggttattatt tatctttttc cttgtgacag
    62641 gattccagta tttctattct gggaataact caggaggaag tcagcaaatc aactatactg
    62701 agttggtaca agaaattacc gatggtaatg taaaagaatt aacttaccaa ccaaatggta
    62761 gtgttatcga agtttctggt gtctataaaa atcctaaaac aagtaaagaa ggaacaggta
    62821 ttcagttttt cacgccatct gttactaagg tagagaaatt taccagcact attcttcctg
    62881 cagatactac cgtatcagaa ttgcaaaaac ttgctactga ccataaagca gaagtaactg
    62941 ttaagcatga aagttcaagt ggtatatgga ttaatctact cgtatccatt gtgccatttg
    63001 gaattctatt cttcttccta ttctctatga tgggaaatat gggaggaggc aatggccgta
    63061 atccaatgag ttttggacgt agtaaggcta aagcagcaaa taaagaagat attaaagtaa
    63121 gattttcaga tgttgctgga gctgaggaag aaaaacaaga actagttgaa gttgttgagt
    63181 tcttaaaaga tccaaaacga ttcacaaaac ttggagcccg tattccagca ggtgttcttt
    63241 tggagggacc tccggggaca ggtaagactt tgcttgctaa ggcagtcgct ggagaagcag
    63301 gtgttccatt ctttagtatc tcaggttctg actttgtaga aatgtttgtc ggagttggag
    63361 ctagtcgtgt tcgctctctt tttgaggatg ccaaaaaagc agcaccagct atcatcttta
    63421 tcgatgaaat tgatgctgtt ggacgtcaac gtggagtcgg tctcggcgga ggtaatgacg
    63481 aacgtgaaca aaccttgaac caacttttga ttgagatgga tggttttgag ggaaatgaag
    63541 ggattatcgt catcgctgcg acaaaccgtt cagatgtact tgatcctgcc cttttgcgtc
    63601 caggacgttt tgatagaaaa gtattggttg gccgtcctga tgttaaaggt cgtgaagcaa
    63661 tcttgaaagt tcacgctaag aacaagcctt tagcagaaga tgttgatttg aaattagtgg
    63721 ctcaacaaac tccaggcttt gttggtgctg atttagagaa tgtcttgaat gaagcagctt
    63781 tagttgctgc tcgtcgcaat aaatcgataa ttgatgcttc agatattgat gaagcagaag
    63841 atagagttat tgctggacct tctaagaaag ataagacagt ttcacaaaaa gaacgagaat
    63901 tggttgctta ccatgaggca ggacatacca ttgttggtct agtcttgtcg aatgctcgcg
    63961 ttgtccataa ggttacaatt gtaccacgcg gccgtgcagg cggatacatg attgcacttc
    64021 ctaaagagga tcaaatgctt ctatctaaag aagatatgaa agagcaattg gttggcttaa
    64081 tgggtggacg tgtagctgaa gaaattatct ttaatgtcca aactacagga gcttcaaacg
    64141 actttgaaca agcgacacaa atggcacgtg caatggttac agagtacggt atgagtgaaa
    64201 aacttggccc agtacaatat gaaggaaacc atgctatgct tggtgcacag agtcctcaaa
    64261 aatcaatttc agaacaaaca gcttatgaaa ttgatgaaga ggttcgttca ttattaaatg
    64321 aggcacgaaa taaagctgct gaaattattc agtcaaatcg tgaaactcac aagttaattg
    64381 cagaagcatt attgaaatac gaaacattgg atagtacaca aattaaagct ctttacgaaa
    64441 caggaaagat gcctgaagca gtagaagagg aatctcatgc actatcctat gatgaagtaa
    64501 agtcaaaaat gaatgacgaa aaataaccct gagagaggct ggagcctc
//
LOCUS       DAVKDZ010000003        62508 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000003 DAVKDZ010000000
VERSION     DAVKDZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 62508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62508
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.003"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(<1..215)
                     /locus_tag="VWZ30_000146"
     CDS             complement(<1..215)
                     /locus_tag="VWZ30_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU9333969.1"
                     /translation="MWMEELPNGKYKFFERYKDPYTEKLKKVSVTMEKKTPQARNQAA
                     ILLQEKINKKLSTKQVESITFEEIYNL"
     gene            complement(365..961)
                     /locus_tag="VWZ30_000147"
     CDS             complement(365..961)
                     /locus_tag="VWZ30_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEU9333970.1"
                     /translation="MKQERKVLGILAIIFGALALLGSWMPIINNFSFILAILALIFGL
                     IGFAVNRKRPKTLAIIGTVLAVVSIAIVLVTQAMYAKSLKELGKNVEETVSSVSSSIE
                     SSQKEEDAKFNWTKEQFDALQVGDIINYGAGGTNYDDVASVHGEPNNVTTSSVNDHDS
                     KTVSYTSTGSKYKSVILSFSKQDDGSFLLTSKVSSGLE"
     gene            complement(1013..1393)
                     /locus_tag="VWZ30_000148"
     CDS             complement(1013..1393)
                     /locus_tag="VWZ30_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEU9333971.1"
                     /translation="MTLAKLCEEYQVELCLFDGSNWHSSGFYNPDTNVLAIDHNLTPE
                     QQIQVALHELGHKDHTLSEYQNARLRCENEADRNMIHHLVKDALESLDDPTEFDYLKF
                     MSYYNLKTMTNEIMVKEEYLSLVN"
     gene            complement(1404..1571)
                     /locus_tag="VWZ30_000149"
     CDS             complement(1404..1571)
                     /locus_tag="VWZ30_000149"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333972.1"
                     /translation="MNHVQVKRPKENNSEYDYMLRLSNESIHQFEQKKKRQESQLKSY
                     PAIKSYYRVAN"
     gene            complement(1568..2044)
                     /locus_tag="VWZ30_000150"
     CDS             complement(1568..2044)
                     /locus_tag="VWZ30_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333973.1"
                     /translation="MTLAKLHFNDQGNLNGGILTNISLAQIQSTLVDNFPNSTTRTRN
                     FQALSTFLKNLDSNIITRIWLDGSFCTQKENPKDIDVLLFFKHSPDIEKYLKDIKEKA
                     STCLDIYQIGDKDHIEKTRPIYSHMAHNETYWMGQFGFDRNRNHKGIIELKTENLK"
     gene            complement(2320..2559)
                     /locus_tag="VWZ30_000151"
     CDS             complement(2320..2559)
                     /locus_tag="VWZ30_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333974.1"
                     /translation="MTITINFTEKNSYITDYLNKHGINTTTMDFDDFMALMEDIEDAR
                     AADQAYMEYLADPNPKTYTTDELLESLGMTREDIA"
     gene            complement(2559..2927)
                     /locus_tag="VWZ30_000152"
     CDS             complement(2559..2927)
                     /locus_tag="VWZ30_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9333975.1"
                     /translation="MFQTFDRIKELAKKQGLSINSLEEKLGYSRNTIYNLKSSKPSTE
                     RISEIADYFNVSTDYLLGRTDNPAIAGSDEFAQVNGQIIDLRKAAANTMLFDGKPLNE
                     DDIDFITSVLSAHFKSKGER"
     gene            3228..3419
                     /locus_tag="VWZ30_000153"
     CDS             3228..3419
                     /locus_tag="VWZ30_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333976.1"
                     /translation="MPNMDGGRQKIRDYLKEHNLTMATLAVQYSMARQDVTNILNGKL
                     KNPQANQFIARVIEDFKIR"
     gene            3446..3625
                     /locus_tag="VWZ30_000154"
     CDS             3446..3625
                     /locus_tag="VWZ30_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333977.1"
                     /translation="MEEDIRVHMPYEVFKNLLVRAGRIKREEGKQITWTNNTAPFTKE
                     QRREIDELYEQFAED"
     gene            complement(3601..4152)
                     /locus_tag="VWZ30_000155"
     CDS             complement(3601..4152)
                     /locus_tag="VWZ30_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333978.1"
                     /translation="MSEVFTTTLISVGATVLTNLIIHGFAYQQKKIENKNKLEEKKVD
                     FLNTQLSQDRDLYISTFQNFASISGKTVSYVESRLDDAPPIDLDIITNFDDIFYKTYL
                     FLDSDEEQKKFIYFRNVLRYQAGYPHPDGLYIWDVQFEEQSYDPDTIEEPRYIFDRLN
                     ECLLIANKHINDKLHIQSSANCS"
     gene            complement(4641..4844)
                     /locus_tag="VWZ30_000156"
     CDS             complement(4641..4844)
                     /locus_tag="VWZ30_000156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333979.1"
                     /translation="MTKSDLGPYLDAVTNEDGTLLICKTEQGAYIGDFNPSCDEEDFV
                     LTYEDVSVSLSYAQVLSATLLKV"
     gene            4911..5114
                     /locus_tag="VWZ30_000157"
     CDS             4911..5114
                     /locus_tag="VWZ30_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333980.1"
                     /translation="MRPKRYPYSGKKESTFVKADPELVEKLLRNTSFLECLQKKPINF
                     QIDSEEFKRLSYKAIHDTSQVTQ"
     gene            complement(5269..5436)
                     /locus_tag="VWZ30_000158"
     CDS             complement(5269..5436)
                     /locus_tag="VWZ30_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEU9333981.1"
                     /translation="MANTPIKPGTDNQKPGRYVEVGPRGGKVTNGHTATIGKGDRLPP
                     TSAKGNGWKKV"
     gene            5553..5696
                     /locus_tag="VWZ30_000159"
     CDS             5553..5696
                     /locus_tag="VWZ30_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333982.1"
                     /translation="MEDKIIELADYFISESTTYREAKIACEKLLKQVSHEIELRAMES
                     RTV"
     gene            5693..5863
                     /locus_tag="VWZ30_000160"
     CDS             5693..5863
                     /locus_tag="VWZ30_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333983.1"
                     /translation="MKEAVKEFLKFRSRFTKIEWFEINQAIEARLNQKADQLKLDDLD
                     LEIISSRLEKVI"
     gene            complement(5856..6095)
                     /locus_tag="VWZ30_000161"
     CDS             complement(5856..6095)
                     /locus_tag="VWZ30_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333984.1"
                     /translation="MTEIHACLCGNWVNLSADDDCVMGPNMANPYIWWEENAELYSPI
                     SKPEANSMYHQDYIYIHYRGADYRIHPMFIQIVSR"
     gene            6214..6486
                     /locus_tag="VWZ30_000162"
     CDS             6214..6486
                     /locus_tag="VWZ30_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333985.1"
                     /translation="MEVVEIVRIKDVIIEKVSANDEELKRIFGCSKRQAGERRREMQK
                     LPSQQKHLLDSGQLVTIKGFYEYLQYRGTKAWKKEMETRKKMRSAG"
     gene            6483..6677
                     /locus_tag="VWZ30_000163"
     CDS             6483..6677
                     /locus_tag="VWZ30_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333986.1"
                     /translation="MNLLSRIKNYFSEEVKETNLDWKEVALDLNQSLIETQEKLQEAN
                     QEIADLKKIVAIYKEKEKEK"
     gene            6674..6865
                     /locus_tag="VWZ30_000164"
     CDS             6674..6865
                     /locus_tag="VWZ30_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333987.1"
                     /translation="MMEYIYLVIIVGIGLWSLVNKLDDHAEMKQKERQLMANNVARMN
                     LRNSDKQFTYDVEPPEGLK"
     gene            6877..7218
                     /locus_tag="VWZ30_000165"
     CDS             6877..7218
                     /locus_tag="VWZ30_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333988.1"
                     /translation="MTQAEQIREYYREHPAASCDEVAEVVGTTKSNVSANLAKDIKAG
                     RCVRLEDKSYDYSPYFNHTQALTELVDWKNDTRREWVDMLTRAAEKETDSNVMRLLIK
                     EANKLMKEVTK"
     gene            7219..7449
                     /locus_tag="VWZ30_000166"
     CDS             7219..7449
                     /locus_tag="VWZ30_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333989.1"
                     /translation="MVRNKLTDLTNTLFAQLETLDDRDLTADELKTELQRSKQMVAIS
                     GQILQAGQLALDAEKFKDKVGEVNAPIALLEG"
     gene            7463..8185
                     /locus_tag="VWZ30_000167"
     CDS             7463..8185
                     /locus_tag="VWZ30_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU9333990.1"
                     /translation="MHEICPGRLTPEVTRLLNEKFGTTYTKTQIGEVRRRLGLPVGKV
                     YQGKLLTKEQHDYLVSIQKNKISRDVANEMNLKFGLSLTEKQIKSYRRNNNLHSGLTG
                     RFEKGQTPHNKGKKYPNMPKNSGQFKKGNRPPNYVPVGTINYTTNGYPKEKIGEPNQW
                     VLKHRKVWEDHHGLIPKGYSIVFLDGDKTNYDISNLACLSKNEIARMNQNHLFTSNAD
                     LTKTGIGLTKLTNKIREVEKNG"
     gene            8178..8660
                     /locus_tag="VWZ30_000168"
     CDS             8178..8660
                     /locus_tag="VWZ30_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HEU9333991.1"
                     /translation="MASLYELTGQFLTIYQLDIDDETKTDTLEAIDWQEQFEQKAEGY
                     AHVIKNLEADVAMYKAEEESFKAKKQVAQKKLDYVKDNIMAAMNITGQTEVKSGALII
                     KIAKNPESVKVNEDDLPKKYFTKKVTLAPDKKTLKELLKSGKKVKGAKLVRTEKLVIK
                     "
     gene            8660..9019
                     /locus_tag="VWZ30_000169"
     CDS             8660..9019
                     /locus_tag="VWZ30_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333992.1"
                     /translation="MELMNKTRVTDSLAVVIGLESIEVLVTEGFLFDVAIRFVKVDET
                     NLDQGNEKPVFTPECKLVTVAKYKEKPIFESEEDIRKFEKQAKEVKSLFAFAKVNKQN
                     WFNTALYPGVLTEKVGV"
     gene            9019..9504
                     /locus_tag="VWZ30_000170"
     CDS             9019..9504
                     /locus_tag="VWZ30_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000696057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333993.1"
                     /translation="MKILAIDPSSNKIETSTTGVVLLDNARLVDSWVVSYGMRGFADW
                     FHEIGTNLEFDVVIVEEFKARDNDKSKDNSVAETIAYIQLCYPGAILQFNAGYKSDIP
                     NDLLKILDLWKFEKSHHQDIRAAARLGLFWAMRNDIEEVVHDIGKVVSEYHNNAKKVA
                     S"
     gene            9467..10660
                     /locus_tag="VWZ30_000171"
     CDS             9467..10660
                     /locus_tag="VWZ30_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HEU9333994.1"
                     /translation="MSITITLRKWQAEAIKRSEHLSNGIFLEALGGRGKTICALAIAK
                     HKKAKKIIITNNRLAILNGWIDAVKFMNFDKGVEIIIQTDRYLQNQVKKGHKLDCDVL
                     IVDEWQNMSSDKQVALYRKIKRKYTIGLSATPIRKKGQNFYPLEKTVFGWATPNNKFD
                     WQKTHGKMVYDPFSYSKEKWEDFQNYESYISSLPNFFRWEEIEGIENAVENNGFEIKF
                     YRKRVASGNPEKLAEFRKLNLVTVDGKTAMAKQSFGRKTFERYLNQTGVAVDFPKLKP
                     VNADTPLMLQLDGLIERAPHDMLIVSKSKQIVNVISERHPEIGIWTGDIQEGLYKKSV
                     VATSQVLGVGVDGLQHKYQTIVVLDPVEEGSGEYDDYRQLLWRITGSRQQHDVNVIEF
                     YYKES"
     gene            10678..10995
                     /locus_tag="VWZ30_000172"
     CDS             10678..10995
                     /locus_tag="VWZ30_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333995.1"
                     /translation="MNKTTEMIVFRSRKTGEFLNSYKDRSSLAFAADFCSLEYCLKLP
                     RKKYEDNKKTYKALAAAFDCEIVAVEAEYKLTYPNGSEVEPIKRDRSSIEDMIKDIIG
                     GVL"
     gene            10995..11821
                     /locus_tag="VWZ30_000173"
                     /pseudo
     CDS             10995..11821
                     /locus_tag="VWZ30_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885998.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            11841..12347
                     /locus_tag="VWZ30_000174"
     CDS             11841..12347
                     /locus_tag="VWZ30_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333996.1"
                     /translation="MSLLDIAKSIKKEGFDPRKDSANGPAPIPAGTYPVVLKKATFNV
                     SDKGWESLGYQFEIRGGDYSGRSEFATFGTLTEWNGKNLDWAVERTMKFFIKALVLAG
                     DSMQGNEEDGKALEEALQRKAVGSYYNLVISVTKGKDGREFRNYDLEEEEAQPLTEAD
                     IDEDDLPF"
     gene            12520..13341
                     /locus_tag="VWZ30_000175"
     CDS             12520..13341
                     /locus_tag="VWZ30_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001139684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEU9333997.1"
                     /translation="MPSMKEYALQYQKLGFSVIPINPKNKMPLIEFADKPAMTASEIE
                     RFWDGYPNANIALRTTNFFVIDIDKHGKENGFESLKKWEHLNLIEPTLQAKTASGGKH
                     LLYFKRDDEPITQMIKFLPGVDIKAHENNYIIVAPSATENGQYEWDLEKSAEGGTIVT
                     PSRDLIRAIKKQYGKTHGYRYDGKDGLRDLARRSQTRDRTQTTDLFETIALGFGDEGG
                     RNDKLAKFVGGLLYRAVDDGVVVQLARLANANSPNPLPEKEMMRTIESMIKKDRR"
     gene            13344..14681
                     /locus_tag="VWZ30_000176"
     CDS             13344..14681
                     /locus_tag="VWZ30_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9333998.1"
                     /translation="MIGNVVSIDSQPKMITTAKGDIKANSPSNVLMSFKADDQLSIYL
                     KHNDFSQEHELLKDIKIGNTLFKKGELPSNFDSVVKVYFESVLGVAFSNQAMLDGMET
                     FFSERSYNPVIEYMERATEKWDGRNRIDRMLQVYLGAEDIPLVSKIAQMWLVGAVAKV
                     YDPYVKFDYVLDLVGGQGVGKTSLLQKLGGEWYTDAVTDFSNKDNYDIMLKSLIVNDD
                     EMVASNRMSFAETKAFISKTSLRYRKPYMKRTEEFAKNFILARTTNQKEYLKDKTGER
                     RFLPVMADSKRQKKHPMEIEPETIEQIWGEAVTIYRAGADLMFDENTEDELNIYREQF
                     MYRDEVELQVLEYLDMPVPENWQNWSIQQQHQYTSKYFDNSSDFDPGSKKLDKVSTRE
                     MMYNLFMRNSNDRKLSTKINMIMDNHPDWKKSVFRAGGKSTKGFVRVKDSEKN"
     gene            14981..15136
                     /locus_tag="VWZ30_000177"
     CDS             14981..15136
                     /locus_tag="VWZ30_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9333999.1"
                     /translation="MSYTVTLFFDNMVDETHFFKKESDAAKCKAQLESKYRGNRMYKV
                     KQEKLEE"
     gene            15133..15606
                     /locus_tag="VWZ30_000178"
     CDS             15133..15606
                     /locus_tag="VWZ30_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEU9334000.1"
                     /translation="MNKQELIEKYKELENSSFDIAAIVVCQLILKDLEQLDEPKPVKV
                     QQFVADFIAEQKKLGHTLSYSIDASMSDIVAEWYWDNSELFALAWIFGYEVEEEKRYT
                     VVTKATKQPLYYNAMDKKLFFSMGGLATKFTRKQLKEADLGWVFDCEGIEIEEVE"
     gene            15606..15779
                     /locus_tag="VWZ30_000179"
     CDS             15606..15779
                     /locus_tag="VWZ30_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334001.1"
                     /translation="MEDEQNILETQLILGKQVLEIVLDLLKDDSKIGVVLPLNINDRE
                     FTITVEKEVTDRD"
     gene            15842..16732
                     /locus_tag="VWZ30_000180"
     CDS             15842..16732
                     /locus_tag="VWZ30_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU9334002.1"
                     /translation="MKFLNLFAGIGGFRLGMESAGHECIGFCEIDKFARASYKAIHDT
                     KGEIELHDITTVSDDTIRGIGSVDIICGGFPCQAFSIAGNRRGFEDTRGTLFFEIARF
                     ASILRPKYLFLENVKGLLNHENGVTFETIISTLDELGYNVEWQVLNSKNFGVPQNRER
                     VFIIGHLRRERTRRIFPLSGKNQSTSNQSVMKIGNINPSGNGMNGEVYQADGLAPTLT
                     TNKGEGQKIAINSYHENNHLSDGFRIRKLTPKECWRLQGFPDWTFDKAQEVNSNSQLY
                     KQAGNSVTVNVIAAIAKELS"
     gene            16833..17249
                     /locus_tag="VWZ30_000181"
     CDS             16833..17249
                     /locus_tag="VWZ30_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334003.1"
                     /translation="MIPKFRVWVKIGKRMVFSDDILAIDYENKEIVTQQVYFESGLAV
                     ERDIYCYDFDDIELMQSTGLKDKNGKEVFVGDIIKCTRGCLHEVYLEKEYGGTFIGGM
                     PSIYLKGLLNGYAWTEDEEIIDNVYENPELLEVTHE"
     gene            17242..17421
                     /locus_tag="VWZ30_000182"
     CDS             17242..17421
                     /locus_tag="VWZ30_000182"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334004.1"
                     /translation="MNKRQRKKKILNGLNKEERYHRTHCPVCDSEAGLFDRYFNTYGF
                     CSEYCGYEYYGISRL"
     gene            17442..17756
                     /locus_tag="VWZ30_000183"
     CDS             17442..17756
                     /locus_tag="VWZ30_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334005.1"
                     /translation="MTLFDEMQQLSSESHAKWFERYFEKYNLEQKLKTSAQKGYTGYL
                     INVWSVRDEYLRNRLGDERTLEALRELLGAGFTVKYKLYLSKNIFTGQDFVSNKKIHI
                     TW"
     gene            17831..18292
                     /locus_tag="VWZ30_000184"
     CDS             17831..18292
                     /locus_tag="VWZ30_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEU9334006.1"
                     /translation="MNKAKELLKELQNLDMDIQSRIDEINELEAGLLSSPKWSDVKVQ
                     GGQARKVDDVYTQLVVMKEAIEQDTKEVINRKLQLGRMINRLKNPKHRTILRMTYINK
                     MYVDDICDSMRGISSPTYYRLKKQAVKELDIILSELIVNDSNCTGMKSKIC"
     gene            18317..18399
                     /locus_tag="VWZ30_000185"
     tRNA            18317..18399
                     /locus_tag="VWZ30_000185"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18348..18350,aa:Tyr,seq:gta)
     gene            19524..19964
                     /locus_tag="VWZ30_000186"
     CDS             19524..19964
                     /locus_tag="VWZ30_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEU9334007.1"
                     /translation="MSGLRIKQKRFADEYIISGNATEAYKKAGYRVSSDRVAGVEGHK
                     LLKNPKIKSYIDEQLKQLDSEKIADQQEVLSYLTSVMRGETQEQTLISIGELGQTITD
                     INVGAKDRIKAAELLGKRHRLWTDKVEADVSGTVVFANESDIPD"
     gene            19942..21240
                     /locus_tag="VWZ30_000187"
     CDS             19942..21240
                     /locus_tag="VWZ30_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEU9334008.1"
                     /translation="MSQTYQIKQSDIVIDLPKTVGAGYGQFWRSRNLYRVVKGSRGSK
                     KSKTTALNYVVRLLKYPWANLLVIRRYSNTNKQSTYTDFKWACNVLGVTHLFKFNESL
                     PEITIKATGQKILFRGLDDELKITSITVDVGSLCWAWFEEAYQIETEDKFSTVVESIR
                     GSLDVPDFFKQITVTFNPWNERHWIKRVFFDEETSRADTFATTTTYRCNEWLDEVDIK
                     RYEDLYHTNPRRARIVCDGEWGVAEGLIYENVTVKDFDKDELLRDLANKLCIGLDFGF
                     THDPTALCCSLINDTTKEIYVFDEAYKVGLITKEVAKMIKDKGYHRSQIIADSAELRL
                     IEELRSEHGITRIKESRKGKDSIMAGVSKLQGYAIYVHPDCKNIMDEFYSYCYQRDKE
                     GNWLNKPEDKNNHLMDALRYSLQCIEGGKATVRRRSDYGL"
     gene            21252..22721
                     /locus_tag="VWZ30_000188"
     CDS             21252..22721
                     /locus_tag="VWZ30_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEU9334009.1"
                     /translation="MYQYLTYPRDGYDEGSLKKDLIYKLITIRNTESSHLKKLKSYYL
                     GKHAILKHTRRNVNAPNYQTVANHAKDIADMATGYFMGNPIKYNNTADGDIDELLTAF
                     DGAEIDQVDAQNALNMAIYGRAYEYIYAKEGLTELDSTSIDPENTFMVYDDSIERKPL
                     FAVYYYEVKDDTKDTTKYQAEVFTENLHYHMVLRSTDSGTTQSEEATPHNLGQIPIIE
                     YRNNHFAIGDYEQQISLIDAYNSLMGNRVNDKEQAVESILVLYGTQLADTPEDAKVAM
                     KILSEEGLLELPGDSARAEFLKNTLDESATEILRTALKEDIYTFSHVPNLTDENFAGN
                     TSGVAMEFKLMSLEMITKTKEANYKRGLRQRIAIFAHYLGMKQIALESHSIVPQFSRG
                     LPKNLLEISQIVNNLEGKVTNRQLISLLPFVEDPDAELEALEEEKKKNMEDMPMFNQD
                     NTKPEDEVEDEESGVLGEEESQSDLPTDGQGRKAGRPVR"
     gene            22630..24181
                     /locus_tag="VWZ30_000189"
                     /pseudo
     CDS             22630..24181
                     /locus_tag="VWZ30_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795723.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            24495..25019
                     /locus_tag="VWZ30_000190"
     CDS             24495..25019
                     /locus_tag="VWZ30_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEU9334010.1"
                     /translation="MSEEINATVSTESTETVDTQENVDTVQEEKHERTFTRAEIGKML
                     SAERSKWEAEQEAKENEAKKLAKMNADEKQKYQLDQREQELADREKAIARKELTAEAK
                     AMLSERDLPVELVNVVDLTNAETVSESITSIQKAWEESVQKGVSERMKGSAPIKNAQT
                     VQQEVTEKWRKDFL"
     gene            25042..26067
                     /locus_tag="VWZ30_000191"
     CDS             25042..26067
                     /locus_tag="VWZ30_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEU9334011.1"
                     /translation="MAFEELNTAESRKKHLGIIEDVLAVNSYSTPLVTSSDAVTLQGR
                     SFTVATGNTTELKDYKRNKDNEFDHVEVEEKVYTLDEEKYWGRFVDQLDERDSNGQVN
                     INYVIARQAAEVVAPYLDELRFGAALGNVSDNVAMGKTAGANNAYNAVLDVSEKLDEL
                     GITKERLLFVTPSFYKAIKSEIVRLPHGDADKKVLGKGYVGELDDYTVYKVPSKFLKG
                     VNALATAPGVVTSPVQVDNTKYNDNIPGRFGELVEQLLYTGAFVLEHFKKYIITIADS
                     KPAAKKSAQGKVVNRAKAWKTGTTYKKGESVTHADKVYVAVKDISNSATAPDTDTTNW
                     KEKTGKK"
     gene            26077..26265
                     /locus_tag="VWZ30_000192"
     CDS             26077..26265
                     /locus_tag="VWZ30_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334012.1"
                     /translation="MKFKIKQDFYDWESNVKRLAGEELEITEERYAELADNFASNGVA
                     ISDVLEEILPEPEFFEED"
     gene            26267..26578
                     /locus_tag="VWZ30_000193"
     CDS             26267..26578
                     /locus_tag="VWZ30_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEU9334013.1"
                     /translation="MSIELLKKMTGEEDTQLLMLLQTRATNLILSETNRTSLTPALSL
                     LIPEVAIELHNRSGAEGEHSRTEGGIAVVYGENGLSTGLLQRIRMHRLARVAGHVFEA
                     E"
     gene            26559..26897
                     /locus_tag="VWZ30_000194"
     CDS             26559..26897
                     /locus_tag="VWZ30_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334014.1"
                     /translation="MFLKQSRLKPYPMRRFEKTVTEEGVAKEGYVKEAETIRLELWPA
                     SSKLQSELYGERVNDILNANANKSATIKVKDGVCIDSQTEVTHRVISKKVYTHHQVLE
                     LERVRATRGR"
     gene            26872..27384
                     /locus_tag="VWZ30_000195"
     CDS             26872..27384
                     /locus_tag="VWZ30_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEU9334015.1"
                     /translation="MSGLLGADRLIAKCRRLASKKTGEDIVLRAVHNAAIKVVQADAR
                     RLAPARDGELIISIKTRAKMDGDKAIGEVYTNLKYAPYVEFGTGPIGQANHSGISPEV
                     SVTYKSNPWYVHEDQINVGPYHFQKIGEFYKMYGQPAQPYLYPALRDNQERVSKNISN
                     YVRRKIREQI"
     gene            27384..27752
                     /locus_tag="VWZ30_000196"
     CDS             27384..27752
                     /locus_tag="VWZ30_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334016.1"
                     /translation="MINIKPVIYKELQKVADNVTDTYPSDWENFPVVIFLEEQNKPGD
                     WFDDKEQKSSIRYKVDIFDDTSTSELAVKINQIFESLGLRRTDCQDVPDPSHLRHKVM
                     RFEGVVDLDSELVFQFRMEN"
     gene            27755..28168
                     /locus_tag="VWZ30_000197"
     CDS             27755..28168
                     /locus_tag="VWZ30_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU9334017.1"
                     /translation="MLANGIKLAFSETKGNYQNLVGLKEVPEFGIEPEKVENTTLADT
                     VKKYEFGIGDAGELKYKFAYNNSSATAPYRVLRKAADGKKKLYFEQTYPDGTKVTFEG
                     QVSVKLGGGGVNAVIEFTLKIALQSELTFVDGIGG"
     gene            28173..28553
                     /locus_tag="VWZ30_000198"
     CDS             28173..28553
                     /locus_tag="VWZ30_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334018.1"
                     /translation="MALKYTTWKVTDEKELKLRLTSHQAATVEEKIGMNLLKIFMPEA
                     GEEFTLPPLKVMLLLVHGALQQYEHGYSFEDVYDLYDEYVDNGGDQTTFMTEVLMPLF
                     EVSGFTPRGSKGKKASKKKMTVVE"
     gene            28592..28942
                     /locus_tag="VWZ30_000199"
     CDS             28592..28942
                     /locus_tag="VWZ30_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334019.1"
                     /translation="MFLDIGGKPLDFWDLTVLEIREMIESYNRVKIQERKEKIIDSYI
                     LSRMITNHVSLLLSNDAKIAELWEYAPDLFVEEKQAVEQERQRQALLLHKERMRDFAE
                     RHNRKRKEEVNGNS"
     gene            28929..29474
                     /locus_tag="VWZ30_000200"
                     /pseudo
     CDS             28929..29474
                     /locus_tag="VWZ30_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            30814..32535
                     /locus_tag="VWZ30_000201"
                     /pseudo
     CDS             30814..32535
                     /locus_tag="VWZ30_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841383.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32544..32894
                     /locus_tag="VWZ30_000202"
     CDS             32544..32894
                     /locus_tag="VWZ30_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334020.1"
                     /translation="MAQLTINGVAVKPPKSFQVGIQDIDGETGRNANGDMVRDRITTK
                     RKLDCEWGMLTQEEMSQLLNAVSAVFFEVSYPDPVKGQTTGTFYVGDRTAPSYSFTEK
                     FKPWSGAKFNLVER"
     gene            32899..36096
                     /locus_tag="VWZ30_000203"
     CDS             32899..36096
                     /locus_tag="VWZ30_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEU9334021.1"
                     /translation="MDALIRRQFDRAMFAKDRTLAIRVGDYASRDIKEASFEYGYIKG
                     DTYKPGGTCAGSGKITFTSIITTFNKLDILHPEIGLLVGTTYQWVKMGEYFINDIEID
                     RNRNTTTLELMDGMFKLNREYVTDLHFPAEVREVIQEICLKTGIELANDYFGISAMRY
                     HIEQVPEGKKLSFRDMLSAMTQMIGMSCFFNREGKMEIRDLTESNITINADSYFLYGL
                     TKSEIEYQISGITCKTDKKSLTVGMKTGRSLELDNVFMTQSALNDLYYKLKNLTYYPY
                     NLNYQGHLLLEVGQWVTIQTNKKETFKVPVLSQSFTFKGGLRGRISADSKAGNDTQYS
                     YEGTITKQIKQQDGIEAKIQAQIEAADKDSDQKVDKIKKDFNDQVELAKARAEEVKQE
                     LSDTINQRFNSFDNGPLKETKRKAEEALRNAGASTLLAQEVKRIGLDSVARLGAFKSQ
                     TTSAQTALSGDLDALKQTVTSEVNQASEYRRTTTEVLSRMTGQMDGFATKSEVRQDVA
                     GLTETFAKLKTDTNNLISGAKSEITLAKTEFQKTADGLSAKMSAVERYVNQDGQRQEA
                     LQRYTREESARQVSAVREQISRDYVGKSAYQEDVRGLERRFSAISTQTNNDIARKIAQ
                     YKQTVDGQFASITSQIAGKANQTDFQRVKETSKLYERILGNSKNGIANTIARMAMTHQ
                     LFQVEVSKNDHLKTVQRQLAGSWSVQNINSAGDLISGINLGANGHNRFVGKLTHITGE
                     TLIDRAVIKSAMVDKLKTANFEAGSVTTTILDAEAVTADKVRFDAAFIRKMTANDAFI
                     DQLTSKRIFSTKVESVISSSTFLEAYQGRIGGFTIGRFAQGRGRWISGINQFSVGMGN
                     GEGGGYNGENTAFWANWGYSWNSPGPNAWYVTTSGNMYCRNGADFHGKVDFSNSSRAN
                     FYGNTTFSRSPVFSNGIELGSKDVFGDGWNPKGGRNAVVWWNQVGSGSVKYWMEQKSD
                     RRLKENITDTAVKALDKINRLRMVAFDFIESKKHEEIGLIAQEAETIVPRIVSRDPEN
                     PDGYLHIDYTALVPYLIKAIQELNQKIEKMEKTIA"
     gene            36093..36209
                     /locus_tag="VWZ30_000204"
     CDS             36093..36209
                     /locus_tag="VWZ30_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HEU9334022.1"
                     /translation="MNNNMLTNIALKAIQELALENRKRTDRLENLENERRTA"
     gene            36190..36393
                     /locus_tag="VWZ30_000205"
     CDS             36190..36393
                     /locus_tag="VWZ30_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334023.1"
                     /translation="MNAEQLNQALQMTISEMSTTSTNSMITSNVLSIQLDEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            36396..36746
                     /locus_tag="VWZ30_000206"
     CDS             36396..36746
                     /locus_tag="VWZ30_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334024.1"
                     /translation="MAKTIQNTDNLLDLTKITEPFDLASALRYMKESGEFIRCKNVND
                     DFYMYRDVQKRPVFVNGRRQFKDVETVWAFNQWGGTITTINVAILLNQEFYIMKFDAE
                     GNPDWTDPTVEPKE"
     gene            36755..37054
                     /locus_tag="VWZ30_000207"
     CDS             36755..37054
                     /locus_tag="VWZ30_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334025.1"
                     /translation="MPIEEAEKIAQSQVAWAILFILLFFIIIRYLIKTSDKREKKIMD
                     LHEQSKADSNRREERLMTHLEKTTTELTTITHAVGDIQKEMVRMNDRMEEIEKGE"
     gene            37058..37390
                     /locus_tag="VWZ30_000208"
     CDS             37058..37390
                     /locus_tag="VWZ30_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEU9334026.1"
                     /translation="MQQITEIITNGAISILVILAGVVVRAVKEYLVKKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKIRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            37394..38350
                     /gene="lytA"
                     /locus_tag="VWZ30_000209"
     CDS             37394..38350
                     /gene="lytA"
                     /locus_tag="VWZ30_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU9334027.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHWR
                     KDPELGFFSHVVGNGRIMQVGPVNNGSWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPYLASWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSD
                     RWKKHTDGNWYYFDQSGEMATGWKKIAEKWYYFDVEGAMKTGWVKYKNTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(38603..39571)
                     /locus_tag="VWZ30_000210"
     CDS             complement(38603..39571)
                     /locus_tag="VWZ30_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9334028.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWTLAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFTPSQLLGDGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            39707..40564
                     /locus_tag="VWZ30_000211"
     CDS             39707..40564
                     /locus_tag="VWZ30_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEU9334029.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKTIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            40551..41330
                     /locus_tag="VWZ30_000212"
     CDS             40551..41330
                     /locus_tag="VWZ30_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334030.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEALPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            41354..42706
                     /locus_tag="VWZ30_000213"
     CDS             41354..42706
                     /locus_tag="VWZ30_000213"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU9334031.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGELLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPKALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     KNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKKTGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            42780..43154
                     /locus_tag="VWZ30_000214"
     CDS             42780..43154
                     /locus_tag="VWZ30_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEU9334032.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            43156..44004
                     /locus_tag="VWZ30_000215"
     CDS             43156..44004
                     /locus_tag="VWZ30_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9334033.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASSQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            44281..45048
                     /locus_tag="VWZ30_000216"
     CDS             44281..45048
                     /locus_tag="VWZ30_000216"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU9334034.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDSDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            45060..46244
                     /locus_tag="VWZ30_000217"
     CDS             45060..46244
                     /locus_tag="VWZ30_000217"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU9334035.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSRDKLNRLFDLETDFTFESSEQAWSALLWALEIENAQSFLKFWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            46241..48112
                     /locus_tag="VWZ30_000218"
     CDS             46241..48112
                     /locus_tag="VWZ30_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9334036.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHNLKKELSGGVAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQD
                     FNLLVQVKDRIGIVGDNGVGKSTLLNLIAGSLESTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKTFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIEALEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(48255..49439)
                     /gene="mntE"
                     /locus_tag="VWZ30_000219"
     CDS             complement(48255..49439)
                     /gene="mntE"
                     /locus_tag="VWZ30_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEU9334037.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            49743..52439
                     /locus_tag="VWZ30_000220"
     CDS             49743..52439
                     /locus_tag="VWZ30_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEU9334038.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVLLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKARDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHILTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEISKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            52452..53243
                     /gene="yghU"
                     /locus_tag="VWZ30_000221"
                     /pseudo
     CDS             52452..53243
                     /gene="yghU"
                     /locus_tag="VWZ30_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
     gene            53359..53769
                     /locus_tag="VWZ30_000222"
     CDS             53359..53769
                     /locus_tag="VWZ30_000222"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9334039.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKCYETIRV
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            53781..55376
                     /locus_tag="VWZ30_000223"
     CDS             53781..55376
                     /locus_tag="VWZ30_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334040.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            55442..55606
                     /locus_tag="VWZ30_000224"
     CDS             55442..55606
                     /locus_tag="VWZ30_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEU9334041.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            55685..56413
                     /gene="yaaA"
                     /locus_tag="VWZ30_000225"
     CDS             55685..56413
                     /gene="yaaA"
                     /locus_tag="VWZ30_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU9334042.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQAVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(56780..57328)
                     /locus_tag="VWZ30_000226"
     CDS             complement(56780..57328)
                     /locus_tag="VWZ30_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9334043.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGASALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQVVGDFTAARVNDSAWADGKL
                     VLEETVLNSLDKQAQDLVNAIK"
     gene            57649..58137
                     /locus_tag="VWZ30_000227"
     CDS             57649..58137
                     /locus_tag="VWZ30_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEU9334044.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            58134..59321
                     /locus_tag="VWZ30_000228"
     CDS             58134..59321
                     /locus_tag="VWZ30_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9334045.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     ENN"
     gene            59314..59679
                     /locus_tag="VWZ30_000229"
     CDS             59314..59679
                     /locus_tag="VWZ30_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9334046.1"
                     /translation="MIDHFEIKVKDLQILEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            59695..61041
                     /gene="asnS"
                     /locus_tag="VWZ30_000230"
     CDS             59695..61041
                     /gene="asnS"
                     /locus_tag="VWZ30_000230"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU9334047.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            61194..61484
                     /locus_tag="VWZ30_000231"
     CDS             61194..61484
                     /locus_tag="VWZ30_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU9334048.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIKDFREGLYHIVNVEANDDVALKEFDRLSKINADILRHMIVKIDA"
     gene            61496..61966
                     /gene="ssbA"
                     /locus_tag="VWZ30_000232"
     CDS             61496..61966
                     /gene="ssbA"
                     /locus_tag="VWZ30_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEU9334049.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTVGAYSAPTANYSAPTNSVPDFSRDENPFGATNPLDISDDDLPF"
     gene            61998..62237
                     /locus_tag="VWZ30_000233"
     CDS             61998..62237
                     /locus_tag="VWZ30_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU9334050.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            complement(62279..>62508)
                     /locus_tag="VWZ30_000234"
     CDS             complement(62279..>62508)
                     /locus_tag="VWZ30_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9334051.1"
                     /translation="PLFQTVFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLIKRNA
                     FGFRNFENFKKRISIALNIKKERTKFVLSRA"
BASE COUNT        19683 a        11210 c        14294 g        17321 t
ORIGIN      
        1 aaaaggttat agatttcttc aaatgtaatg ctttctactt gttttgtgct aagtttttta
       61 tttatcttct cttgcaacaa gatagcagct tgatttcttg cctggggagt tttcttctcc
      121 atggtcactg aaactttttt taatttctcg gtatacgggt ctttgtatcg ttcaaagaat
      181 ttgtattttc cgttgggaag ttcttccatc cacattgcgt ttacctcact tttttgttaa
      241 aatgggtata agaaaaagag ctttttaatg ctttttttct taccactagc ctcacgctct
      301 cggtctgcaa acttctgagc gtggggcttt tttgagttgt ttccaaaatg gaaacagttg
      361 gtttttattc tagacctgaa ctaactttag aagtcaacaa gaaagaaccg tcgtcttgtt
      421 ttgaaaacga aagaataacg ctcttgtatt tgctaccagt agaagtatat gatactgtct
      481 tactgtcgtg gtcatttact gaactagttg ttacattgtt aggttctccg tgaacgctag
      541 caacgtcatc gtagttagtt ccaccagctc catagttgat aatatcacca acttgaagcg
      601 catcaaactg ttcttttgtc cagttaaatt tagcatcttc ttctttttga gatgattcga
      661 tagaagaact tacagagcta acagtctctt caacgttttt acctagttct ttcaatgatt
      721 tagcgtacat tgcttgagtt actaatacaa tagcaataga tacgactgct agaaccgtcc
      781 caataatagc tagtgttttt ggtctttttc gatttactgc aaaaccaatt aaaccgaaga
      841 taagagctaa aatagccaaa atgaatgaaa aattattgat gataggcatc catgagccaa
      901 gtagagccag cgctccaaaa ataatagcta aaatacctaa aactttgcgt tcttgtttca
      961 taatgaaacc tctctatcag cttttagtgt ggatcagttg ttgcacatat ttttaattta
     1021 ctaatgataa atactcttct ttaaccatga tttcatttgt catggttttt agattgtagt
     1081 aagacatgaa tttgaggtaa tcaaactctg tggggtcgtc taagctttct agcgcgtctt
     1141 ttacgagatg atggatcata tttctatcag cttcgttttc acagcgtagt cgagcgttct
     1201 ggtactctga gagtgtatgg tctttgtgtc caagttcgtg tagggcgact tggatttgtt
     1261 gttcaggagt caagttgtgg tcaatagcga gtacgtttgt gtctggattg tagaaaccgc
     1321 tactgtgcca gtttgaaccg tcgaagagac aaagttctac ttgatattct tcgcagagtt
     1381 tagcgagtgt catagttctc cttttaatta gccactcgat aataactttt aatagctgga
     1441 tagcttttta gttggctttc ttgacgtttc tttttttgtt caaattgatg aatggattca
     1501 ttactgagcc gtagcatgta atcatattca ctgttatttt cttttggtcg tttaacttgg
     1561 acgtgattca ttttagattc tccgttttta attctataat tcctttgtga tttctatttc
     1621 tatcaaaacc aaattgtccc atccagtatg tttcattgtg agccatgtgg gaatatatag
     1681 gacgagtttt ttcaatatga tctttatcgc ctatttgata gatgtctaag caggtacttg
     1741 ctttttcttt aatatctttt agatatttct ctatgtctgg agaatgttta aaaaatagta
     1801 agacatctat gtcttttggg ttttctttct gggtgcaaaa gcttccgtct agccatatac
     1861 gcgtaatgat attgctatct agatttttta agaaagttga gagagcttgg aaatttctag
     1921 ttctggtagt ggagtttggg aagttatcta ctagagttga ttgtatttgt gctagtgaga
     1981 tatttgttag tattcctcca tttaggttac cttgatcatt gaagtggagt ttagcgagtg
     2041 tcatagttct cctttgttat ttataaacct ctctgcggtg ggcgatgtct acggctaaga
     2101 cgactagttt atcgtcttgg atatcacaga tgatgcgcta atcgccaact cggtatcggc
     2161 agtaacctga taggtttcct tttagtagtt tagcgtgttg ataggggttg cttgtgttgt
     2221 ctacgttttt tgtcatccac gagatgattt ttcgtctagt tgctccatcg agttttttga
     2281 gttgtttttg tgctttgctt tcatagacga gtcggtacat taggcgatgt cctctctggt
     2341 cattccaaga ctttccaaga gttcatctgt tgtatatgtt tttggattag ggtcggctaa
     2401 atactccata taggcttggt cggctgctcg tgcgtcttcg atatcttcca tgagtgccat
     2461 gaagtcgtca aaatccatgg tcgttgtgtt gataccgtgt ttgtttaggt agtccgtgat
     2521 gtaggaattt ttttctgtga agttgatagt gatagtcatt agcgttctcc tttgcttttg
     2581 aagtgggcgg ataggacgga tgtgatgaag tcgatatcat cttcatttag tggtttcccg
     2641 tcaaataaca tggtgttggc tgctgctttg cgtaagtcta tgatttgtcc gtttacttgg
     2701 gcaaattcat cactcccagc aatagcaggg ttatctgtac gacctagaag ataatcagtg
     2761 gacacattga agtagtcagc tatttctgaa attcgttcag tagatggttt tgaacttttt
     2821 aaattataaa tggtatttct actgtaacca agtttttctt ccaatgaatt tattgaaagt
     2881 ccttgctttt tggcaagttc tttaattctg tcaaatgtct gaaacattga tttttcaacc
     2941 tttctgagaa catgacaaaa aatatttaat atttctcatt aaaacacttg acaaagttaa
     3001 tgcgaagtat taaaatagtt tttgtaagtt aatgagttag taaaaaacga agttaaaact
     3061 tatctaaaaa taaatagctt tggcgagcaa gaaaattgat agatataagg ttttatcaag
     3121 gtttttaatt atgctttcat tttaatacta tacattaatt ttgtcaagca ttttataaaa
     3181 caatttacta actttttttc ttacttttaa agaaaggagg gaggcagatg ccaaacatgg
     3241 atggtggacg tcaaaaaatc agagattatc tgaaagagca caacttgacg atggcgacgc
     3301 tagcagtaca gtatagcatg gctcgtcagg atgtgacgaa tatcctgaat ggaaagctaa
     3361 aaaatccaca agcgaatcag ttcatcgctc gtgtgattga agattttaaa attcggtaaa
     3421 aatattggtt taagtaggag gaaagatgga agaagacatc agagttcaca tgccttacga
     3481 ggtatttaaa aacctgcttg ttagagcagg cagaataaag cgtgaagaag gcaagcagat
     3541 aacttggaca aataataccg ctccgtttac aaaagagcaa cggagggaaa tagatgaact
     3601 ctacgagcag ttcgcagaag attgaatgtg gagtttgtca ttgatatgtt tgttagcaat
     3661 caataaacat tcattaagcc tatcaaaaat atatctgggt tcttcgattg tgtctgggtc
     3721 ataggactgt tcttcaaatt gcacatccca aatataaagc ccatcggggt ggggatatcc
     3781 agcttgataa cgtagcacat ttctaaaata gatgaatttt ttttgctctt catcgctatc
     3841 taaaaataga taggtcttat aaaagatatc atcgaagttt gtgatgatat ctaaatcaat
     3901 cggaggagca tcgtcaagtc ttgattctac gtatgagacg gttttaccac taatcgatgc
     3961 aaaattttga aaagttgaaa tatataaatc tctatcctga cttagttggg tatttaagaa
     4021 atcaaccttc ttttcttcga gtttattttt attttcaatc tttttttgct ggtaagcaaa
     4081 accgtgaata atcaaatttg ttaaaacagt tgcaccaaca gaaatcaatg ttgttgtaaa
     4141 tacttcagac atacaaatac ctcagataat atttttaaat cattatatca caaacagaaa
     4201 ggagtaggat ggaagaattt attgatgctc ttgaaaaaga aaaagaccac cttgaaaaaa
     4261 tcattgaggt agtcagctct ggtggtaaat ttctgagatt gccgtatcaa aaaagtcacg
     4321 ctcgattagt gagaatctga aattgatttc tcaaaatctt gatagactga gctgtttata
     4381 taaccaaaga ggagaaagaa tgacagacag agaactattt aagttaccag aagattatgt
     4441 agaatctact ggacttgaca agattacatt tgaagtacct tttgaattgt ttacgaaaat
     4501 tctaaaaggg tacggacata agttagcgtg ggaggatttc cgacaaataa aaatccaccc
     4561 aagcactaga acaaaaaaga cggttggaca gtgtcagttt ttgtttagta tttggatgaa
     4621 tgatcatttg aagccagcga ttaaaccttc aaaagagttg cagaaagaac ctgtgcgtaa
     4681 gacaaagaaa cagaaacgtc ttcataagtt agcacaaaat cttcttcatc gcatgaaggg
     4741 ttgaaatcgc caatgtatgc accttgctct gttttgcaga ttagaagagt accatcttcg
     4801 tttgtaactg catctagata aggtcccaaa tcagacttag tcataagtta tcctcctttc
     4861 tgcttatatt atagcagaaa tagaggttag aaatagaaag gagtaggaag atgagaccaa
     4921 aacgatatcc gtatagtggg aaaaaagagt ccacctttgt aaaggcagac cctgagttag
     4981 ttgaaaaact tttaagaaac actagttttc ttgagtgttt acaaaaaaag cctatcaatt
     5041 ttcagataga ctcagaagaa tttaagcgtc ttagctataa agccattcat gatacttctc
     5101 aagtaactca ataggaggaa ggaataaaat gattcaccat tatataactc actatgccag
     5161 caatgggaaa gattacgccg aagcatggat tcaaattgat tttttgggaa tgtgcttttg
     5221 tgtatggaaa aagcgtacaa ccattgaacg attgtacgca aacgaagatt agactttttt
     5281 ccaaccgttg cctttagcag atgtcggagg gagccgatca ccttttccga tagttgcggt
     5341 atgaccatta gtaacttttc cgccacgagg tcctacctct acatagcgac caggtttctg
     5401 attatctgtt ccaggtttta ttggagtatt tgccatacta tctcctcctt tctattggaa
     5461 ttttgactaa aacggtgaga ggtcctagtc aagagtatta tagcaattta ggaggatatt
     5521 acatcagtct tgaggctgat ataggaggtt gaatggaaga taaaattatc gaacttgctg
     5581 attacttcat cagcgaatct acaacgtaca gagaagctaa aatagcgtgt gagaagctat
     5641 tgaaacaagt cagccatgag atagaactca gggcgatgga aagtaggaca gtatgaaaga
     5701 agcagtaaag gaatttctaa aattcaggag ccgatttaca aaaatagaat ggtttgaaat
     5761 taaccaagct atcgaagctc gtttaaatca aaaagccgac cagttgaaac tggacgactt
     5821 agatttagaa atcatttcta gcagactaga aaaagttatc tagaaacgat ttgaatgaac
     5881 attggatgga tacgatagtc agcgccacga tagtgaatgt agatataatc ctgatggtac
     5941 atcgagtttg cttcaggttt agaaattggt gagtagagtt ctgcattttc ttcccaccaa
     6001 atgtaaggat tagccatatt tggtcccatt acacaatcgt cgtcggctga taggttcacc
     6061 caatttccgc aaagacatgc gtgaatttca gtcataatat tacctccttt ctggtttcat
     6121 tatagcagaa aaggagatag caaaaaagca cctgacggca atcaggcgca tgacaaaatt
     6181 attcaagaaa attataacac gaaaggagca aaaatggaag tagttgaaat tgtaagaatt
     6241 aaagatgtga tcattgaaaa agtctctgct aatgatgaag agttaaaacg tatctttgga
     6301 tgttcaaaac gacaagcagg agagcgaaga agagaaatgc aaaaactccc tagtcagcaa
     6361 aaacatcttt tggatagtgg acaacttgta acgattaaag gtttctatga atacttgcaa
     6421 tatcgtggaa ctaaagcttg gaaaaaagaa atggaaacaa gaaagaaaat gaggtcagca
     6481 ggatgaacct actatcaaga atcaaaaact atttttcgga agaggtcaaa gaaactaatc
     6541 tcgactggaa agaggtcgct ttagacctca atcaatcact aattgaaaca caggaaaaac
     6601 ttcaagaagc gaatcaagaa atcgcagact tgaagaaaat cgtagcaatc tacaaagaaa
     6661 aggagaaaga aaaatgatgg aatacattta cctggtaata atcgtaggaa ttggactatg
     6721 gtcgctagta aataaactag atgaccacgc tgaaatgaaa caaaaagagc gccagctgat
     6781 ggcaaacaat gtcgcacgga tgaatctgag aaattcagat aagcaattta cttatgatgt
     6841 agaaccgcct gaagggttga aataaggagg agaaacatga ctcaagcgga acaaattagg
     6901 gaatattata gagagcaccc tgctgcctca tgtgatgaag tggctgaggt tgtcggtaca
     6961 acaaaaagca acgtaagtgc aaacctggcc aaagacatca aggcaggcag atgcgttcgc
     7021 ttggaagaca agtcatacga ctactcccct tactttaatc acacacaagc gctcactgag
     7081 ttggttgatt ggaagaatga taccagacgt gagtgggtgg atatgctgac aagagcagca
     7141 gaaaaagaaa cggatagcaa tgttatgcgt ttgttaatca aagaagcaaa taaattgatg
     7201 aaagaggtga cgaagtaaat ggttcgaaat aaattgacag atttaacaaa tactttgttt
     7261 gctcaattgg aaaccttgga cgatagggat cttactgcag atgaattaaa gacggaactc
     7321 caacgttcaa aacagatggt cgcaatctca ggtcaaatct tacaagcagg tcaattggcg
     7381 ctagatgctg aaaaattcaa agacaaggta ggtgaagtca atgccccgat cgctttgctg
     7441 gaaggatgag tatacagagt acatgcatga gatatgccct ggccgattaa ctcctgaagt
     7501 aaccaggtta ctaaatgaga aatttggtac gacctatacc aagactcaaa taggagaagt
     7561 acgcagacgt ttagggttac ctgttggaaa agtatatcaa ggtaaattgt tgacaaaaga
     7621 acaacatgat taccttgtgt caatccaaaa aaataagatt tctcgcgatg tcgcaaatga
     7681 aatgaaccta aaatttggat tatcactgac tgagaaacag attaagagtt atcgaagaaa
     7741 taataatcta catagtggtt tgacaggaag attcgagaaa ggtcagactc ctcacaataa
     7801 ggggaagaag taccccaata tgccaaaaaa cagcgggcag ttcaaaaaag gtaatcgacc
     7861 tccgaattat gtacctgtcg gtactatcaa ctacacaaca aacggttatc caaaagaaaa
     7921 gattggagaa cctaatcaat gggttttgaa acaccgcaag gtttgggagg accatcacgg
     7981 gctgatacca aaagggtact caatcgtttt tctggacggt gataaaacaa actatgatat
     8041 ttcaaatctg gcatgtttat ctaagaacga aattgctaga atgaatcaaa atcatttatt
     8101 cacgtccaac gctgatttga ctaaaacagg tattggacta acaaaactta caaataaaat
     8161 cagagaggta gaaaaaaatg gctagtttat acgaactaac aggtcagttc ctgacaattt
     8221 accaattgga tatcgatgac gaaacaaaaa cggacacact tgaggccatc gattggcaag
     8281 aacaatttga acagaaagca gaaggatatg cccatgttat caagaatcta gaagccgacg
     8341 tggccatgta caaagctgag gaagagagct tcaaagccaa gaaacaggtg gcacagaaaa
     8401 agctggatta tgtaaaggat aacattatgg cagctatgaa tatcacgggg caaaccgaag
     8461 ttaagagtgg tgccctgatt ataaaaattg ctaagaatcc agaatcagtc aaggtcaacg
     8521 aagacgacct tccgaaaaaa tattttacaa aaaaagtgac gcttgcgccg gacaaaaaaa
     8581 cactcaaaga gttgcttaaa tctggcaaga aagtcaaagg tgcgaaactt gtccggacag
     8641 aaaagttggt gattaagtaa tggaattgat gaataaaaca cgagtaacag attcactagc
     8701 agttgtgatt ggactagaat cgattgaagt acttgttact gaaggttttc tatttgatgt
     8761 tgcgattcgt tttgtaaaag tagacgaaac aaatcttgat caaggaaatg aaaagccagt
     8821 attcactccg gaatgcaagc tggtcacagt tgctaaatac aaggaaaaac ctatctttga
     8881 atcggaggaa gatattcgaa aatttgagaa gcaagcaaaa gaagttaaat cgctatttgc
     8941 ctttgcaaag gtaaataaac aaaattggtt taacactgcc ctttatccag gagtgctgac
     9001 tgagaaagtt ggtgtttgat gaaaatttta gctattgatc caagcagtaa taaaattgaa
     9061 accagcacaa caggagttgt cttgttggat aatgcaagat tagttgatag ctgggttgtc
     9121 tcttatggta tgagaggttt cgctgattgg tttcacgaaa tcggaacaaa tcttgaattc
     9181 gatgtagtta ttgttgaaga atttaaggcg agggataacg acaagtcgaa agataatagc
     9241 gtggcagaaa ccatcgccta tatccaactt tgctatccag gtgccattct tcaattcaat
     9301 gcaggttaca agtcggatat tccaaacgat cttttgaaaa tcttagacct ttggaaattt
     9361 gaaaaaagtc atcatcaaga tattcgagca gcagcaagac ttggattatt ttgggcaatg
     9421 agaaatgata ttgaagaagt ggttcatgat atcggaaagg tggtgagtga gtatcacaat
     9481 aacgctaaga aagtggcaag ctgaagcgat taaaagaagt gaacatttat ctaatggaat
     9541 ctttttagag gctcttgggg gcagaggcaa aactatctgt gcacttgcta ttgcaaaaca
     9601 taaaaaagct aaaaaaatca tcatcacaaa caatcgacta gctattctga atggttggat
     9661 agatgcagtc aagtttatga attttgataa aggtgttgag attatcattc agacagatag
     9721 atatcttcaa aatcaagtca aaaaggggca taaattagat tgtgatgtgc tgatagtaga
     9781 cgaatggcag aatatgtctt ctgacaaaca agtggcctta tatcgcaaaa taaagcgaaa
     9841 atacacgata ggtctttcag cgacaccaat tcggaaaaaa ggacaaaatt tctatccgct
     9901 tgaaaaaacg gtatttggtt gggcaacccc aaataataaa tttgactggc aaaagactca
     9961 tgggaaaatg gtctatgatc catttagcta ttcaaaagag aagtgggaag attttcaaaa
    10021 ttatgaaagt tatatctcga gcttgcctaa tttcttccgc tgggaagaga ttgaaggaat
    10081 tgagaatgca gttgagaata acggttttga gattaagttt taccgaaaga gagtcgcctc
    10141 tggcaatcca gaaaaacttg cagaatttag aaaactaaat cttgtaacag tggacggcaa
    10201 aactgcaatg gccaagcaat cgtttggaag aaagaccttt gaacgctacc ttaatcaaac
    10261 aggcgtagca gtcgattttc caaaattaaa gccagtaaat gcggatacgc cattgatgtt
    10321 acaacttgac ggtttaatcg aacgagcacc acacgatatg ttgattgtca gtaaatctaa
    10381 gcagattgtc aacgtcatta gcgagcgcca tcctgaaatt ggaatctgga cgggcgatat
    10441 tcaagaagga ctttataaga aatccgtggt tgctactagt caagtgttag gtgtcggagt
    10501 agacggcttg caacacaaat accaaactat tgtcgtattg gatccagtag aagaaggttc
    10561 tggagaatat gatgattatc gacaattgct ctggcgcata acaggaagtc gtcagcagca
    10621 tgatgtaaat gtaattgaat tttattataa agaaagttaa aaaaagagga aaacaaaatg
    10681 aataaaacaa ctgaaatgat cgtatttcgt agccgtaaaa ctggagaatt tcttaattct
    10741 tacaaggaca gaagttcttt agcatttgca gctgactttt gcagcttgga atattgtttg
    10801 aagcttcctc gtaaaaaata cgaagacaac aaaaagactt acaaggctct tgctgcagct
    10861 tttgactgtg aaattgtcgc agttgaagcg gaatacaaat tgacctatcc gaatggatca
    10921 gaagttgaac ctatcaagcg tgaccgttca tcaattgagg acatgattaa ggatattatt
    10981 ggaggggttc tctaatggca tttacacttc cagcaaataa accacaagtt cctaaagata
    11041 ccccagaaat tttttcatct acggcgaaac catgagcgga aagtcttatc ttgcaaatga
    11101 attcccaaat ccaatcgttt tgaacacaga cgggaatgca gaagctaaca ctgttccaag
    11161 cattcagctg atcaatgaaa aagatgacaa gggacgaatt accaattcag taattaagca
    11221 gcttggagat atcttgcttg ctctccagac acagaagcac tcttatgaaa cagtcgttat
    11281 tgatgtaatt gacgatgtta ttgaaatgat taagattgca gtttgtgatg aattaacccc
    11341 agttggtaaa cctcgcttga aatccttgtc ggaaattcca tacggcaaag gatacgactt
    11401 ctttaaccaa gctatcacag aattagtcat tgacctcaaa gcattgccaa tgaatgttat
    11461 ttacatcagc cgtcaggtat ctgaatatga tgacaatggc aatgccacca aagacaagcc
    11521 aagcttgaaa gataagtatg tcaatcttat caatggaaac tctgatttga tgatccacac
    11581 tgaaaaactc ggcaacaact acaaccgtga ggttgaccgc aagcgtaaga cctattatgc
    11641 ggaccaggtt gatgacaagg ccatcttgaa aatcttggca actatccgtg gggctgttga
    11701 gcctgcaaag ggcaagctag ctcctaaaaa agaagcagct aagacaacta aaccagctaa
    11761 gatcgaaaaa acaaaagagg cacctaagaa agaagttgac tctgatgatg aactatttta
    11821 agaaataaag gagaatacac atgagcttac tagatatcgc aaaatcaatc aaaaaagagg
    11881 gctttgaccc acgcaaagac agcgccaacg gtcctgcacc aatcccagct ggtacttatc
    11941 cagtagtcct gaagaaagca accttcaacg tatcggacaa aggctgggaa agccttggtt
    12001 atcaatttga aatccgtggc ggtgattaca gtggacgctc tgaatttgca acatttggca
    12061 cactgactga atggaacggt aagaaccttg actgggcagt tgaacgcact atgaaattct
    12121 ttatcaaagc cttggtcctt gctggcgaca gtatgcaagg aaatgaagaa gacggtaaag
    12181 ccttggaaga ggctctacaa cgtaaggcag ttggctctta ctacaacctt gttatctctg
    12241 tgactaaggg gaaagatggc cgtgagttcc gaaactatga ccttgaagaa gaagaagcac
    12301 aaccgctgac tgaagctgat atcgatgaag atgacctccc tttctaaaaa atagcaagtt
    12361 ttgggtcatt gatgaaactg atgaaagatt aggaccattc aatacatttg aagaggctta
    12421 tcaatcgttg ttattttatt taaaaatgac tgaagatgaa tatcagtcaa actatacggc
    12481 ccaggaactt gtttatattt acaaagagga gaaaaaacca tgccgtcgat gaaagaatac
    12541 gcattacagt accaaaagtt agggttctca gtcattccaa tcaatcctaa aaacaagatg
    12601 cctttaatcg aatttgctga taaaccagcc atgactgcaa gtgaaatcga acgattttgg
    12661 gatggatacc caaatgcgaa tattgcttta aggacaacta acttctttgt cattgatatc
    12721 gataagcatg gcaaagagaa tggctttgag tcgttaaaaa aatgggaaca tttaaattta
    12781 attgaaccaa ctcttcaagc caaaactgct agtggtggaa agcatctgtt atatttcaaa
    12841 agagatgatg aaccaattac tcagatgatt aaattcttgc ctggtgttga tatcaaagcg
    12901 cacgagaata actatatcat agtagcacct tcagcaacag agaatggcca gtatgagtgg
    12961 gatttggaga aatcagcgga aggtgggaca atcgtaactc cttcccgtga tctaatccga
    13021 gcaattaaga agcagtatgg taaaactcat ggctatagat acgatggcaa agatggtctt
    13081 agggatttag cgaggcgttc acaaaccaga gaccgaacac agactacaga tctctttgaa
    13141 accatcgccc ttggttttgg tgatgaaggt ggacgaaatg acaaactagc aaaattcgta
    13201 ggtggtctct tatatcgtgc ggtcgacgat ggtgtagttg ttcaacttgc aagattagca
    13261 aatgcaaata gtccaaaccc tttgcctgaa aaggaaatga tgcgtactat tgaaagtatg
    13321 attaaaaaag ataggaggtg attgtgattg gtaatgtagt aagtattgac tcacaaccta
    13381 agatgataac gactgccaag ggagacatca aggccaacag tccaagtaat gtgttgatgt
    13441 ctttcaaagc tgatgatcag ttgagtattt acctaaagca caacgatttt tcccaagagc
    13501 atgaactcct taaagatatc aagatcggca acactctttt taaaaaaggt gagctccctt
    13561 ctaactttga ttcagtcgta aaagtttact ttgaaagtgt gttaggtgtt gctttctcaa
    13621 accaagcgat gcttgatggc atggagactt tcttttcaga aagatcatac aatccagtta
    13681 ttgagtatat ggagagagca actgaaaagt gggacggcag aaaccggatt gaccgcatgc
    13741 ttcaagtata tctcggcgct gaagatatcc ctttagtttc taaaatcgct caaatgtggc
    13801 tagttggtgc agttgctaaa gtttatgatc catacgttaa gtttgactat gttctggacc
    13861 tggtcggtgg acaaggagtt gggaaaacgt ccctccttca aaaattgggt ggcgaatggt
    13921 atacggatgc cgtaacagat ttctctaata aagataatta cgacattatg ttaaagagtc
    13981 taatcgtcaa cgatgatgaa atggtggcca gtaatcggat gagctttgca gaaactaagg
    14041 cctttatttc taaaactagc ctacgttatc gtaaaccata catgaaacga acagaagaat
    14101 ttgccaagaa cttcatctta gccaggacta ctaatcaaaa agaatacctc aaggacaaaa
    14161 ccggtgaacg tcgttttcta ccagttatgg cagatagtaa acggcaaaaa aaacatccaa
    14221 tggaaatcga gccagagaca attgaacaaa tctggggcga agccgttaca atctatcgtg
    14281 ctggtgctga tttgatgttt gatgaaaata cagaggatga actgaatatc taccgtgaac
    14341 agttcatgta tcgtgatgaa gttgaattac aagtgcttga atatcttgat atgcccgtcc
    14401 ctgaaaattg gcaaaactgg tctattcagc aacaacatca atacacaagt aaatatttcg
    14461 ataatagtag cgactttgat cctggaagca aaaaactaga taaggtctca actcgtgaaa
    14521 tgatgtacaa cttatttatg agaaattcga atgacaggaa gctgtcaacg aagattaaca
    14581 tgatcatgga taatcatcct gattggaaaa aaagtgtttt ccgggcagga ggtaaaagta
    14641 caaaagggtt cgtaagagtg aaagattcgg aaaaaaacta atcggtagca attaaaaaat
    14701 tatcggtagt catcggtagc agttgagggg gagatcggta gcattctacc gataaaatag
    14761 gacatcggta gcacatcggt agcagtctaa ccccttgata ttactgactt ttatttaata
    14821 tttatatata atgctactct tctatctata tttttaaaaa aagtatataa aataatagta
    14881 ataataaaga aagcctataa aatagggatt cttgaaaaaa actttttact ttttagaatt
    14941 tatcggtagc acggtagcag tttagaaaaa agaggtaaaa atgtcataca cagtaacatt
    15001 attttttgac aacatggtag acgaaactca cttttttaag aaagagagtg atgctgccaa
    15061 atgcaaggcg cagttagaaa gcaagtatcg agggaatcga atgtataaag ttaagcagga
    15121 gaaattggaa gaatgaataa gcaggaattg attgagaaat ataaagagct ggagaatagt
    15181 tcatttgata ttgcagcgat tgtagtttgt cagctaattt taaaagactt ggaacagcta
    15241 gatgaaccaa aaccggtcaa agttcagcag tttgtggcgg attttatcgc agaacagaaa
    15301 aaactgggtc atacactgtc ctactcaata gacgcaagca tgtctgatat agttgcagaa
    15361 tggtattggg ataattccga actcttcgca ctagcttgga ttttcggcta cgaggtcgag
    15421 gaagagaagc ggtatacagt agtgacgaaa gcaacaaaac aaccgctata ttataatgct
    15481 atggataaga aactattctt ctctatgggc ggactagcta caaaatttac tcgcaaacaa
    15541 ctcaaagaag cggacttagg ctgggttttc gattgtgaag gaattgagat tgaggaggtg
    15601 gagtgatgga agatgagcaa aatattttag agacacaatt gattttaggt aagcaagttt
    15661 tagaaattgt attggatttg ctaaaagacg attcaaaaat aggggtagtt ctacctttaa
    15721 acataaatga ccgtgaattt acaattactg tagaaaagga ggtcacagat cgtgactaag
    15781 gggtgtttgt ggttatgaag gacgtgtatg acaaggtgaa agtcggggat gaggtgatgt
    15841 tgtgaaattc ttgaatttat tcgcaggaat tggcggtttt cgtttaggaa tggagtctgc
    15901 cgggcatgaa tgtataggat tttgtgaaat agacaaattc gctagagcta gttataaagc
    15961 tatacacgat acgaaaggag aaattgaatt acatgacatc acaacagtat cagatgacac
    16021 tattcgagga atcggaagtg tggacattat ctgtggagga tttccgtgcc aagctttctc
    16081 aattgcagga aacagacgag gttttgaaga tacacgagga actttgttct ttgaaattgc
    16141 taggttcgca tctattctca gacctaaata tctattcctt gagaatgtta aaggattgct
    16201 caatcacgaa aatggagtta cattcgagac cattatctca accttggatg aactggggta
    16261 caacgtggaa tggcaagtgc ttaacagcaa gaatttcgga gtcccccaaa atcgggaacg
    16321 agtgtttatt atcggacatc ttagaagaga acgtaccaga agaatttttc ctctcagcgg
    16381 aaaaaatcag tcaactagta accaatcagt catgaaaatt gggaatataa acccatctgg
    16441 caacggaatg aatggggaag tctatcaagc tgatggtcta gctcccacgc taacaacaaa
    16501 caagggagag gggcaaaaga tagctataaa tagctatcac gaaaacaatc atttatcaga
    16561 tggctttcga atcagaaagc taacacctaa agaatgctgg aggttacaag gttttcctga
    16621 ttggactttt gataaggcgc aggaggtcaa ctctaacagt caattatata aacaagcagg
    16681 aaatagcgtg acagttaatg tcatagcagc aatagcaaag gagttatcat gaacgcacta
    16741 gaaaaagtcg aacaatggtt tatcgaccgt gaaatcaaag atcgaaaagg tcgttggatt
    16801 gatggtgctt tcgtcaaaga ggaggatttg gtatgatacc gaaatttaga gtgtgggtaa
    16861 aaataggaaa acgtatggtt ttttcagatg acattcttgc tattgactac gaaaacaaag
    16921 aaatagtgac acaacaagtc tatttcgaga gtggtctagc agttgaaaga gatatctatt
    16981 gttatgattt tgacgatatc gaactcatgc aatcaacagg acttaaggac aagaacggga
    17041 aagaggtctt tgtcggagat attataaaat gtacaagagg atgtctccat gaagtatatt
    17101 tagaaaaaga atacggtggc acattcatag gcggaatgcc ttccatatat ctaaagggat
    17161 tgctaaatgg gtatgcgtgg actgaagacg aggaaatcat cgacaatgtc tacgaaaacc
    17221 ctgaactttt ggaggtaact catgaataaa cgtcaacgca aaaagaaaat tttgaacggt
    17281 ctgaacaaag aagaaagata ccacaggacg cattgtcctg tctgcgatag cgaagctggt
    17341 ttattcgaca gatattttaa tacgtacggt ttctgctctg aatattgtgg ttatgaatac
    17401 tatggaattt caagattata aaataaagga ttgaggttaa aatgacatta tttgatgaaa
    17461 tgcagcaatt aagctcagaa agccacgcaa aatggttcga gcgatatttt gagaaatata
    17521 acctagaaca aaaactaaaa acttctgctc aaaaaggtta tacaggttat ttaatcaatg
    17581 tttggtcagt tagagacgaa tatctcagga atcgattagg agatgaaaga acgttggaag
    17641 cgttaagaga attattagga gctggcttta ctgtcaaata taagctttat ctatctaaaa
    17701 atattttcac tggacaagat ttcgtttcta acaagaaaat tcacattact tggtaaaaca
    17761 aaaaagccaa gacactctct gtctcagcaa taatctcaat aatattatta tatcacaaag
    17821 gagacagaga gtgaacaagg ctaaagaact attgaaagag ttgcagaatc tggacatgga
    17881 cattcaaagc cgtatagatg aaattaacga gcttgaggca ggtttgctct caagtcctaa
    17941 gtggtcagat gtcaaagttc aaggtggtca agctagaaaa gttgatgatg tctatactca
    18001 gcttgtcgtg atgaaagagg ctatagaaca ggatactaaa gaggttatca acagaaagct
    18061 ccaactaggt aggatgatca ataggcttaa aaatccaaaa catagaacta ttttgagaat
    18121 gacttacatc aataagatgt acgttgatga catctgtgac agcatgaggg gcataagttc
    18181 tcctacttac tatcgtttga agaaacaggc agtaaaggag cttgatatta ttctttcaga
    18241 attgatagta aatgatagta actgtacagg catgaagtct aaaatctgtt aaaatggtag
    18301 tatcaagaat tgaaaagaga ggtctcagaa ttggtagatg gttacctgta atgtcagggg
    18361 gctgtaatgg ccttggaggt tcaaaccctc ccctctcctt tgagtgtttg tgtcccagaa
    18421 tggggtaggc aacaggctta gcattcatat atcactcatt aacttacaaa tggttgcgga
    18481 gcgactggac cttgcatgat tgcgtagcta attatattcc ggataagtta taagctagag
    18541 ggtttgattc cctcagaggt tttaaatgac tacaaaaaat aaaaaaagaa gtcaaaattt
    18601 aatacgcacg caaggttgta gtcgccttgc acttttaggg cttagcctag ataatctgtg
    18661 gtaactcagg aaaaggatgt ttttaaatct atcaaacatc ctgccagcaa tggtcaatct
    18721 aagcaatgta atcttaacta tttcagtttt ggaataggtg ggcgaagtta aagcagaaag
    18781 attccaacgg caaggtgctg aggaaatgca aacgtggcag tttggctgtg aaacgagtct
    18841 ataagaggaa agaggtattt ggttcgaggt gcaacaagag cttaatacca tatcttacaa
    18901 aaattgggcg cctcccaaaa gtatgtaagg tgagttgatt gtccgcaaaa caatcgataa
    18961 caagcaggcg ctgtgcattt tgttcttcaa aagaggatga aacacatggc gatgcgtgtc
    19021 tgtgatagat gaaagatgat ttttatattt taaggctatt caagatagaa aaaaattcga
    19081 aaaaagcaaa agtcatcgcc cgtcgcaaac gaaagtgtac ttcggcaatt agattgcctg
    19141 ctcaagtctc gcaaggataa gagtaaagtc aaagagtaaa gcagcttaga cttttagcgg
    19201 ggtcttcgtt aattgaaaaa tggcttagta gtttgtgatg taagaagtga ttggtctaac
    19261 caatcgtgca tgagtgatac aagtaggaat atttgtggac aagataataa actataagtt
    19321 atcaaaagtc actcgtttaa agcagtagtc tcatgctagt taatggatac atggtagacg
    19381 gattaagtcc tgtttaggga attgaaacgt aggcaggttc gaatcctgtc gttccaattg
    19441 cgattttaat tcgcagagag aggtcttgaa aaggtcgcac atcgtgtggc ttttttgatt
    19501 gtttgaaagg tggtgatgga aaattgagtg gattgagaat aaaacaaaag agatttgcag
    19561 atgagtacat catctcaggt aatgcgacgg aagcctataa gaaagcaggt tatcgtgttt
    19621 ctagtgatag agtggcaggc gttgaaggac ataagttact aaagaatcct aagattaaaa
    19681 gttatataga tgaacagttg aaacagcttg attctgaaaa aatcgcagac cagcaagaag
    19741 tccttagtta tctaacctcg gtaatgcgag gagagacaca agaacagact ttgataagca
    19801 tcggagaatt gggtcaaacg attacggata ttaatgttgg agcaaaagac agaatcaagg
    19861 cagccgaact attaggaaaa cgtcataggc tttggacaga caaagtagag gcagacgttt
    19921 ctgggacggt ggtgtttgca aatgagtcag acataccaga ttaaacaaag tgatattgta
    19981 atcgacctac ctaagacagt aggagctgga tacggacagt tctggcgctc aagaaatctt
    20041 tatcgtgttg taaaaggttc ccgtggttcg aagaagtcca agacaaccgc tttgaattat
    20101 gttgtccgtc ttttgaaata tccctgggcc aacttgcttg ttattcgtag atactcgaat
    20161 accaacaagc aatcaactta tacggatttt aaatgggcgt gtaatgtgtt gggtgtgact
    20221 catttgttta aattcaatga atctttgcct gaaataacca taaaagcgac tggtcaaaaa
    20281 atcctattcc gtggtttgga tgatgaactc aaaatcacat ctatcacggt cgatgtcggc
    20341 agtctttgtt gggcatggtt tgaggaagca tatcaaattg agactgaaga caagttcagt
    20401 acagtagttg agtcaatccg tggtagcctg gatgtacctg atttctttaa acaaatcaca
    20461 gtcacattta acccgtggaa tgagaggcac tggatcaaac gtgtgttctt tgatgaagag
    20521 actagccgag ctgatacatt cgctactaca actacttatc gatgcaatga atggctggat
    20581 gaagtcgata tcaaacgcta tgaggatttg tatcatacga accccagacg tgcgagaatc
    20641 gtttgtgatg gcgaatgggg agttgctgaa ggtttaatct atgagaacgt gaccgtcaag
    20701 gatttcgata aggatgaatt gctacgagat ttagctaata agttatgtat cggtcttgac
    20761 tttggtttta ctcatgatcc aactgctttg tgttgttcgt tgataaatga cacgacgaaa
    20821 gagatttatg tctttgatga ggcgtacaag gtcggtctta taaccaaaga agttgctaag
    20881 atgataaagg acaaaggtta tcatcgctca caaatcattg ctgatagcgc tgaattacga
    20941 ctgattgagg aattaaggtc agaacatggt ataactcgaa ttaaagagag tcggaaaggt
    21001 aaggatagta ttatggcagg cgtatccaaa ttgcaaggat acgctattta tgtgcatcca
    21061 gattgtaaaa acatcatgga tgaattttat agttactgct accagcgaga taaagaaggc
    21121 aactggttga ataaaccaga ggataaaaac aaccacttga tggacgcttt gcgttacagc
    21181 cttcaatgta tcgaaggtgg aaaagcaacc gtccgcagac gttctgatta tggtctatag
    21241 agaggaaaga catgtaccaa tatttaacct atccacggga tggatatgat gagggttctt
    21301 tgaagaaaga cctgatttac aaattgataa cgatacgtaa cactgaaagc tcacatttga
    21361 agaagcttaa aagctactac ttgggtaagc atgctatctt aaaacacacg agacgcaacg
    21421 tgaacgcacc caattaccag acggtagcta atcatgccaa ggatatcgca gacatggcta
    21481 cgggctattt tatgggcaat cctatcaagt ataacaatac tgctgacggt gatatcgatg
    21541 aactacttac agcctttgat ggtgctgaga ttgaccaagt agatgctcag aatgctttga
    21601 acatggctat ctatggtcgt gcttacgagt acatctatgc taaagagggt ttgactgagt
    21661 tggattcaac tagtattgat ccagaaaata ccttcatggt ctacgatgat agtattgagc
    21721 ggaagccttt gtttgcggtc tattactatg aagtaaaaga cgatacgaaa gacactacca
    21781 agtaccaggc tgaggtcttt acagaaaatc tgcactatca catggtgctg agaagtacag
    21841 attcaggaac aactcagagc gaggaggcaa cacctcacaa ccttggtcaa atcccaatta
    21901 tcgagtatcg caacaatcac tttgcgattg gcgactacga gcaacagatt agcttgatag
    21961 acgcttataa ttccttgatg ggtaatcgtg tcaatgacaa ggagcaggca gtagagtcta
    22021 tccttgtctt atatggcaca cagttagcag acactccaga agatgccaag gtagcgatga
    22081 agattctttc tgaagaaggt cttttggaat tgccgggcga tagtgcaaga gccgagttct
    22141 tgaagaatac gctggatgaa agtgctacag aaatcttgcg cacagctctg aaagaggata
    22201 tctacacatt cagccatgtg cctaacttga ctgatgagaa cttcgcaggg aatacatcag
    22261 gcgtagccat ggaatttaag ctgatgagcc ttgaaatgat taccaagacc aaggaagcga
    22321 actataagcg tggattaaga caacgtattg cgatttttgc tcattacttg ggtatgaagc
    22381 agattgcttt agagtctcat tcaatcgttc cacaattcag ccgtggtttg cctaaaaact
    22441 tgctggaaat ctctcagatt gtgaacaatt tggaaggtaa agtgaccaat aggcagctta
    22501 tttctctctt gccgtttgtg gaagaccctg atgctgaatt ggaagccttg gaagaagaga
    22561 aaaagaagaa catggaagac atgccgatgt ttaaccaaga caacacgaaa cccgaagacg
    22621 aggtagagga tgaagaatca ggagtattgg gcgaagagga aagccaatct gatttaccaa
    22681 cagatggaca aggccgaaaa gcaggcagac cagttcgata aggtctatca ggaagccaag
    22741 acttacttgg ataaggaagt caataagatt ttcgataaat tccaacgtga ttatggtcta
    22801 agtcaggtag acgctagaca agtcttgaag aacatgaaag ccaagaaaga cttgaatgaa
    22861 cttcgtaagg tgcttgaagc aagaccgaat gatccaaata tccaaagact actagctgac
    22921 ttagacagtc cggcttattc tttccgtatg aagcgtctag aacgtttgag cgatgattta
    22981 gaccgtatgc gtgaatctat ctatcattcg gagaagacag gctcagatgc cttttatagc
    23041 gacctgatga aggatagtta ctacaaggct accttttgac ctacaacagc agacaggact
    23101 agcatatggc ttttctgggc ttcctgagag cgagattaaa catctacagt ctttcagttg
    23161 ggtaggtgac ggaagtacgt actcaacaaa catctggaag aatacaggga agcttacatc
    23221 aagcataaaa gatgaattac tcataagcct tatgacaggc cgagatacac gagaaactgc
    23281 acaagcaatt gctgagcggt tcaatgtggg gcagaacgat gcaaggcgtt tggttcggac
    23341 agaatcagcc ttttttcata accaaatgga gctactcaac tatgaggaag cggatataga
    23401 gaagtatatc tttgtggccg tcttagacaa gcgtacatca cgcatttgtc aggagcatga
    23461 caatcaggtc tatgataggg ataaggctgt ccctggtgtc aattgtccgc ctatgcaccc
    23521 ttggtgtagg tctactactg tcggatacga tgaggacgca gactacagca agttgaagcg
    23581 cagagcaagg aatccagaga caggtaaagt tgagtacgtg cctgccgata tgacttataa
    23641 agagtggtat agcaagtatg ttgcgaaaga tgtaaaaaat gaaatacaag attataagaa
    23701 aagtgacaaa accgtttcaa gatataatac cccaaaattg ttttctgatg ttagtaacgc
    23761 atgggatgaa attgggaggg gtggattatc gaaagaacaa cttgtagact tgctagaatc
    23821 tgaatatgaa ttaggtaatt tttcgagcga tatagcaaaa ttgataggag taagttctgc
    23881 ttatatagat gttagtagtt tagctacttc attagtgaga catggacaac agtactcctt
    23941 agatgagttt atgttaataa aagaggcggt tcaaaaacct tatttgattc tagataattc
    24001 agagagggtt gaaaaatcaa ttatttcata tgtaaaaata cctaacaaag ataaggtcat
    24061 tatggaagcg gtgatggtgc cacgagatga aatgctagtc attcacttta acaaggtggg
    24121 gattcgtcaa gttaaaaaga atgaaaaaaa tatgtcgacg ctttacaaaa agggaaaata
    24181 atgctatact cttggtaaag atagaggttg agaatctgtc accaacgcgc cacttatagt
    24241 gggtcgagaa atgcaggagc cccgacagtc ctgcctatct tgcgcttaga tagtaatcta
    24301 ggcgcttttt tgttgcaata aaccactata aaccgcatcg aaatctaggc ggttttctta
    24361 tgccctaacc gtatggaatc ccgtacggtt tttatattgt ccaaactgta ccgatgacaa
    24421 taaaagctgt gctgttccgt cgccggacgt aaagcgagac tatcgagtgg cgacgtaatc
    24481 gctggaggac aattatgtca gaagaaatca atgcaactgt atctactgaa tcaactgaga
    24541 ctgtcgacac tcaagaaaat gttgatacag tgcaggaaga aaagcacgaa cgaactttca
    24601 ctcgtgctga aatcggtaag atgctatctg ccgagcgctc taaatgggaa gctgagcaag
    24661 aagccaagga aaacgaagct aagaagcttg ctaagatgaa cgctgatgaa aaacagaaat
    24721 atcagttgga tcagcgtgag caagaactag ctgaccgtga aaaggctatt gctcgtaagg
    24781 aattgaccgc agaggctaaa gcaatgctaa gtgaacgtga cttacctgtt gagttagtaa
    24841 atgtagtcga tttgacaaac gcagagacgg tatctgaatc tatcacctct atccaaaaag
    24901 catgggaaga gtcagttcag aagggagtct ctgaacgtat gaaaggtagt gcacctatca
    24961 aaaatgcaca aacagtccag caagaagtca cggaaaaatg gcgtaaagac ttcttgtaat
    25021 aaaagaaaag aggaaaaata aatggcattt gaagaattaa acacagcaga atcacgcaag
    25081 aaacatcttg ggattattga ggatgtactt gcagtaaatt catattcaac accacttgtg
    25141 acatcaagcg atgcagtaac cttgcaaggt cgctctttta cagtagcaac tggtaacaca
    25201 acagagttga aagactacaa acgtaacaaa gacaacgaat ttgatcacgt tgaagttgaa
    25261 gaaaaggttt atacccttga tgaagaaaaa tactggggtc gtttcgtaga tcaattggac
    25321 gaacgtgact ctaatggtca agtgaatatc aattatgtta ttgcccgtca ggctgcagaa
    25381 gtagtcgctc catatcttga tgaactacgt tttggtgcag cacttggaaa cgtaagtgac
    25441 aatgttgcca tgggtaaaac agcaggagcg aacaacgctt ataatgcggt tcttgatgtg
    25501 tctgagaaac ttgatgagct tggaattaca aaagaacgct tgctcttcgt cactccaagt
    25561 ttctacaaag cgatcaagtc tgaaatcgtt cgtctaccac atggtgacgc agataagaaa
    25621 gttcttggaa aaggatatgt tggtgaattg gatgattaca cagtctataa ggttccttct
    25681 aaattcctga aaggtgttaa tgcccttgct actgctccag gtgttgttac atctccagta
    25741 caagtagata atactaagta caacgataac attccagggc gatttggcga attggtagag
    25801 caattgcttt acactggtgc atttgttctt gaacacttca agaaatacat catcacaatt
    25861 gcagattcta agcctgctgc taaaaaatca gcacaaggta aagttgtaaa ccgtgctaaa
    25921 gcgtggaaaa ctggaacaac ttacaaaaaa ggcgaatcag taactcatgc cgataaagtc
    25981 tatgtagcgg ttaaggatat ttctaattca gcaaccgcac cagacacaga tacaactaac
    26041 tggaaagaaa aaactggtaa gaaataggtc cgagttatga aatttaaaat caaacaagat
    26101 ttctatgatt gggaatcaaa tgtgaaacga ctggcaggag aggaacttga gattactgag
    26161 gagcgctatg ccgagctggc tgacaatttt gccagcaatg gtgtcgctat ctcagatgtt
    26221 cttgaggaaa tcctccctga acctgagttt ttcgaagagg attaatatgt ctatagagtt
    26281 gctgaagaaa atgacaggcg aagaagatac tcagcttctc atgttgctcc aaacgagggc
    26341 tacaaatctt atcttgtcag agactaatcg aacatctttg acacctgctt taagtctctt
    26401 aatacctgag gttgctatcg aactccacaa ccgctcagga gcggaaggag agcattcaag
    26461 gaccgaaggt ggtatagcag tagtctacgg agaaaacggc ctgtctacgg gccttttaca
    26521 gcgtatacgt atgcatagac tagcaagggt ggcaggccat gtttttgaag cagagtagac
    26581 tgaaacctta tccaatgcga cggtttgaaa agactgtcac tgaggaaggt gtcgcaaaag
    26641 aaggatatgt caaggaagct gagacaatcc gtcttgagtt gtggccagct agtagtaaac
    26701 tacagtctga attgtatggc gagcgtgtca atgatatttt gaacgcaaat gccaacaagt
    26761 cagctacaat caaagtgaaa gatggtgtgt gtatcgatag ccagacagaa gtgactcaca
    26821 gggttatttc taaaaaggtc tacacacatc atcaagtttt ggagttagag cgtgtcaggg
    26881 ctactagggg cagataggct catagctaaa tgtagacgat tggctagtaa aaaaactgga
    26941 gaggatatcg tcttacgtgc ggtacacaat gctgctataa aggttgtcca agctgatgca
    27001 agaagactcg caccagcgag agatggagag cttataatta gtatcaaaac tagagcaaaa
    27061 atggacggag ataaggctat aggcgaagtt tacaccaacc ttaaatacgc tccttacgtt
    27121 gagtttggaa caggaccaat aggacaagct aaccattcgg gtatctctcc agaggtcagc
    27181 gtaacttaca agtctaatcc ttggtatgtg catgaagacc aaatcaatgt aggaccgtac
    27241 cactttcaaa agattgggga gttctacaag atgtatggtc aacctgctca gccttatctt
    27301 tatccagctt tgagagacaa tcaagagcgt gtgtctaaga atatttcgaa ttatgtccgt
    27361 agaaagataa gagaacaaat ataatgatca atatcaagcc tgttatttat aaagaattgc
    27421 aaaaggtcgc agataatgtg actgatactt atcctagcga ttgggagaat ttcccagtcg
    27481 ttattttttt agaagaacaa aacaagccag gtgattggtt tgacgacaag gaacaaaaat
    27541 cctctattcg ctataaggtg gatatctttg atgataccag cactagtgag ttagctgtta
    27601 aaatcaatca gatttttgag tctttaggtt tgcgaagaac cgactgccaa gacgtgccag
    27661 atccgtctca tttgagacat aaggtcatgc gctttgaagg tgtcgttgat ttagactcag
    27721 agcttgtttt tcaatttaga atggagaatt aaacatgtta gcaaatggaa ttaaattggc
    27781 ctttagtgaa acgaaaggca attatcagaa tcttgtaggg cttaaggaag tacctgaatt
    27841 tggtatcgag cccgaaaaag tagaaaatac tactcttgca gatacggtga agaagtacga
    27901 gtttggtatc ggggacgcag gagaacttaa gtacaagttc gcttataata attcaagcgc
    27961 aacagctcct taccgtgtat tacgtaaggc agcagatggc aagaaaaaac tctactttga
    28021 acaaacctac ccagacggta ctaaggtcac ttttgaaggt caagtatccg ttaaactggg
    28081 cggtggcggc gttaatgccg ttatcgagtt cacacttaag attgcattgc agtctgaatt
    28141 gacattcgtt gatggaattg gaggttaatt aaatggcgtt aaaatacaca acttggaaag
    28201 ttactgacga aaaagagttg aagctacgtt tgacatctca tcaagctgca actgtggaag
    28261 aaaaaatcgg catgaacttg ttaaagattt tcatgcctga agctggcgaa gagttcactt
    28321 taccgccttt gaaagttatg ctgttgttag ttcatggagc cttgcagcag tatgaacatg
    28381 ggtattcctt tgaagatgtc tacgacctat acgatgaata cgtggataac ggcggagacc
    28441 aaacaacctt catgacagag gttttaatgc cactctttga agtatcgggt tttactccac
    28501 gaggaagcaa gggcaagaaa gcttccaaga agaaaatgac agtagtcgag taatcttaac
    28561 ggtaacgcag attattgaga ggctttaccc tatgtttttg gacatcgggg gtaagcctct
    28621 tgatttttgg gatttaacgg tgcttgaaat cagggaaatg attgaaagct acaaccgtgt
    28681 caaaatccaa gagcgtaaag agaagattat tgactcatac atactttcga gaatgataac
    28741 taatcatgtt tccttattac tgtccaatga cgctaagatt gctgagcttt gggaatatgc
    28801 gcctgatttg tttgtagaag agaaacaagc ggtagaacaa gaacgacaaa gacaagcact
    28861 tttgttgcat aaggaacgga tgcgtgattt tgcagagaga cataatcgaa aaaggaagga
    28921 ggaagtaaat ggcaactctt gacgaattga aagtcatgat tgacgctgag atagcgcctt
    28981 tcaggaaaaa gatgaaagaa gtcgagaatc aggtcaaagg aacatctgac caagtgaaaa
    29041 atgccactgc caaagttcgt gaacagtcga attcaatcgg tagtacgttt ggtaagctcg
    29101 ctaagtttgc tggttttgca atccttggta agaaattgct tgatgttggg atgtattcaa
    29161 cgcagacagc tcttgaagta tcagcgtcta tgaaccaaat taagcgacag atgggtgaga
    29221 gttcgcaatc tttcttaaaa tgggttaacg ataacgctaa tgccatgaat atgggtgtgg
    29281 gcgaggctac taactatggt gcagtctact caaacttatt ttctggattt atcaaagaca
    29341 ccaacaagct aagcgcttat actgctaaga tgttgcagac gtcggcagtt gtcgctgaag
    29401 gctcagggcg cacgattaca gacgttatgg agcggattcg ctcaggtttg ctagggaata
    29461 cggaagcgat agacttttgt cgcaccgctt agaaataggc ggattaagaa cttaccaaaa
    29521 tcggtaaaac tctaaatttt aagtaattaa aacatgacga taccgaggta aactaagcaa
    29581 ttaaaaaggc ttagtcaccg tagagcatag ggattgaacc tgtgcttttt gttttgtcaa
    29641 aaagtataga ataaaatatc cccacgagtg gtgagcacct agacaattcg gttgtaggtg
    29701 aaaatatatg ccgaacttac aagaaattgt aagaagtatg gataaaaagc catgcgataa
    29761 cattattgag aagatctagg aatcaacgtc aatgtggcta tgattgagtc cactgaagcc
    29821 tttaagaagt tcgcaaacgg acaaagctgg caacaattag actaccaaac ccagcaacaa
    29881 atccgcctta tggctatcct ggaacaggct acagccaagt atgggaatac cttgtctaat
    29941 tctgtaaatg gtcgtatcag cctgtttaag tcgcttatga aggacgcagc attgaacctt
    30001 ggtaactcta tgttaccgat tatcaatgcc attatgcctg tcttgaactc ttttgctatg
    30061 gttttgaaga acgtgacggc taaactcgct gagtttatcg ctttgatgtt caacaagaaa
    30121 gcaacagtga aagatggtgt tggtggagca gttggagaca tgggtaacgc catgaaagac
    30181 gctgcaggcg gagcaggaga ccttgctgac gcagtagacg atgctggaga ttcagcagga
    30241 ggacttgctg acaatcttgg agactccgcc aaaaacgcta agaaagctgc taaagagttg
    30301 ctaggtcttt tgggatttga tgagattaac atcttgcaaa aaccaaaaga cgacgatgca
    30361 ggcggttctg gaggcggtgg caaaggtggt aaaggaaagg gaggcggtgg cggacctttc
    30421 aaagacatct tgccagaagt cgagttgacc gacatggaca accaattcaa gagcattttc
    30481 gatggccttg gggataagct gaaagggttg tttgattatc taaaaaaact ctgggattta
    30541 tttaaaaaag gtttttctct atcctttaga tgggatagta ttgaaagatt aaagaatgca
    30601 ctacaaggca tttggaaatc tattaaagat atctttgaag atggtactgt attgcaagca
    30661 gctgcaaggt ttggagaaaa gctagctttt gctttaggac aaacgacggg cgctctcgct
    30721 aacgtaatca tgggtattgc tgtctttatc gctgaaagtc tgaataaatc acttaatgaa
    30781 acgaaactag atatcaaagc atggttaatt cgtatgtttg atattggtgg ggaaatcgtc
    30841 gaaagcgtcg gaaatattgc ccagagtatc ggacaaattt tctacgattc aattacaagt
    30901 gaactagcta caaatatggg cgcaggccta ttcagtgctt tcacatacgc ttttatgggc
    30961 gtgaaagaga taaccgctaa atacacaagg gatataatcg gagctattga agaaactatc
    31021 accgaaaatc aggctggcat aacagaaatg tttacgggtc tttttaaagc tgtagatcct
    31081 attgctcaag ctttatcaag ctcaatgaag aaactttttg aaagtgttaa ttaagtatac
    31141 gatgagcata taaaaccttt gtttgaatca agttccgcct tgatgtcaga tgtagttggt
    31201 gcttttgtta atggatggaa tgataatatc caacctgtcc ttgaaaagat aggtcacggt
    31261 ttcgccgata caatcaaaaa ccatattgaa ccagctttag aaaaaatagg tggcatgatt
    31321 ggaagttttg ccgacttttc taaagcgata aatgaagttt tcggcccagt catttccttt
    31381 attgtagaaa agttaacggt tgtactagcc cctgcaattg aatacataag agaagtttgg
    31441 cgtgttttat ttaacactat ctctgatgtg attggtggta ttgctgatat catcaaaggg
    31501 gtatttgatg tacttacagg acttttaact ggagatggcg aaaaaatcaa agaaggattt
    31561 ttgagtatat ttggcggatt aaaagatata gtagttagcg tctttagtgg cattattgat
    31621 cttgtatctg gtgtattgaa actgctttgg gacgttgttg tcgcaatatt caaaagcatt
    31681 tgggacgcaa ttgtagctat cttttctggt gtcagctctt ggtttggaga aaagttccaa
    31741 ggtgcatggg atgctatcgt taacatcttc agtaatctag gctcatggtt cggacaacgt
    31801 tgggcggatg tgactagtgc tttagctaat atcggggcat ggtttacgaa tatgttccaa
    31861 aaagcatgga ctggcttaac aaacatcttt agcaaactag gttcatggtt tggcgataga
    31921 tggaacgatg ttacaagtgc gctttctaaa gtagcgagct ggtttggcga gatattcgga
    31981 aaagcttttg acgcagtaaa agatgctttt agttctattg gcgacttctt taaaggcgtt
    32041 tgggatactg tcaaaagtat cttcgttaat gctggtcaga tggtcggcga ggcagtaggt
    32101 ggagcgttta agagtgcggt caatgcggtt cttggaacga ttgaaaatgt agtcaatggt
    32161 ttcatcggaa tgattaatgg agttttaggc gttgtcagaa acttacctgg tctaggatgg
    32221 gttggtagtg taagcacagt tagcctccct cgtcttgccc gtggtggtat cgtcgatagt
    32281 ccaacaatcg ccatgattgg tgaagctggt aaagaggcgg tcgtaccact tgaaaataca
    32341 ggatttatcc aaacacttgg acgagtagtc agcagtgcgg tagtaaatgc catggctggt
    32401 gttagtccac aaggtggatt ctctggcgac ggcgacatcg ttatccaaat cgcaggccat
    32461 gagttcggac gggtagccat ccaagaaatc aacaaggaac atgaacgagc aggtcaaacc
    32521 ttgctcaaga tttaggaggt taaatggcac aattgacaat caatggggtg gctgtgaagc
    32581 ctcccaaatc ttttcaagtc ggtattcaag atatcgatgg agagacaggg cgtaatgcca
    32641 atggcgatat ggtgcgtgac cgtatcacga ccaaacgaaa actagactgt gaatggggca
    32701 tgctgactca ggaagaaatg agtcagcttt taaatgctgt ttcagcagtc ttttttgagg
    32761 tctcatatcc tgaccctgtt aaaggtcaga cgactgggac tttctacgtc ggtgatagga
    32821 cagctcctag ctatagcttt actgagaagt ttaaaccttg gtctggcgct aaatttaatc
    32881 tggtagagag gtgagaaaat ggacgcttta atcagacgac agtttgacag agctatgttt
    32941 gccaaggata ggacgctggc tattcgtgtt ggtgattatg cttcacgaga tatcaaagag
    33001 gctagttttg agtatggcta tatcaaaggc gatacttaca aacccggtgg aacgtgtgcc
    33061 ggtagcggta agattacctt taccagtatc attaccacgt tcaataaact ggatatacta
    33121 caccctgaga ttggtctatt ggttgggact acctaccagt gggttaagat gggggaatac
    33181 ttcatcaatg atattgagat tgaccgaaac cgaaacacaa ccacgcttga actcatggat
    33241 ggtatgttta agctcaatcg tgagtatgtg acggacttac attttccagc tgaagtacga
    33301 gaggttattc aggaaatctg cctgaaaaca ggcattgagt tagcgaatga ctatttcgga
    33361 atcagcgcta tgcgttacca tatcgagcaa gttcctgagg gcaagaaact ttcctttagg
    33421 gatatgctga gcgctatgac tcagatgatt gggatgtctt gtttcttcaa cagagaaggc
    33481 aagatggaaa tccgtgattt gactgagtcc aatatcacga tcaacgctga cagttacttc
    33541 ttgtatggct tgaccaagag tgagattgag tatcagatat ctggtatcac ttgtaagacg
    33601 gacaagaagt ctctgacggt cggtatgaag acaggtcggt ctttggaact ggacaatgtc
    33661 ttcatgaccc agagcgcttt aaatgacctg tattacaaac tgaaaaacct aacttactat
    33721 ccgtataatc tcaactacca aggacatttg ttacttgagg ttgggcagtg ggtaaccatt
    33781 cagaccaaca agaaagagac ttttaaagtt cccgtgttaa gtcagagctt tacctttaaa
    33841 ggtggtctga gaggtcgtat cagtgcagat agtaaggccg gaaacgatac ccagtattct
    33901 tacgagggta cgattaccaa gcagattaag caacaagatg gcattgaagc caaaatccaa
    33961 gcgcagattg aagcagcaga taaagattct gaccaaaagg tcgacaaaat caaaaaagac
    34021 tttaacgatc aagtagaact tgccaaagcc agagctgaag aagtcaagca agaactgtct
    34081 gacactatca atcagcgctt taatagcttt gacaacgggc cattgaaaga aactaagcgc
    34141 aaggctgagg aagctttgcg aaatgctggc gcaagtaccc tgcttgcaca ggaagttaag
    34201 cggattggac tggattctgt cgctagactt ggagcgttta agtcgcagac tacgagcgca
    34261 caaacggctc tgtcaggtga cttggacgct ctgaaacaga cggtcacaag tgaggtcaat
    34321 caagcttcag agtaccgcag aacgaccaca gaggtcctta gtcgcatgac tggccagatg
    34381 gacggatttg cgacgaaatc tgaagttaga caagatgtgg ctggtctgac agagacattt
    34441 gctaaactta aaactgatac gaataatttg atttctggag ctaaaagtga aatcactcta
    34501 gcaaaaacag aatttcagaa aacagctgat ggcctatctg ctaagatgtc agcagttgag
    34561 cgttacgtta atcaagatgg tcagcgacag gaagccctac agcgctatac tcgtgaggag
    34621 agcgctcgtc aagtcagcgc ggttcgtgag cagatatcca gagattatgt tgggaaatcg
    34681 gcttatcaag aagatgtgag aggtctagaa cgtcgattta gtgcgataag cacccagacg
    34741 aacaatgata tagctaggaa aatcgctcag tacaagcaga cagtcgatgg acaatttgca
    34801 agtattacgt ctcaaatagc tggtaaggct aatcagactg acttccagcg tgttaaggaa
    34861 accagtaaac tttacgagcg gattctgggt aatagtaaaa acgggattgc aaatacgatt
    34921 gcaagaatgg ctatgaccca tcagctcttt caggttgaag tatcgaaaaa cgaccatctg
    34981 aaaaccgttc agaggcagct ggcaggttcc tggtcggttc aaaacatcaa cagtgcaggt
    35041 gatttgattt cgggcattaa tctaggagcc aatggacata accgctttgt tgggaaattg
    35101 acccacatca ctggcgagac cctgattgac agagcagtca tcaagtctgc catggttgat
    35161 aagctcaaaa cggccaattt tgaagctggt tcggtaacga ctacgatatt agacgctgaa
    35221 gcggtcacgg ctgataaagt gagatttgat gctgcgttta ttaggaaaat gactgcaaat
    35281 gacgctttta ttgaccaact gacatctaaa cgtatcttct ctactaaggt tgagtccgtc
    35341 atttctagtt caaccttcct agaagcctat caaggacgaa tcggtggatt cactattggg
    35401 cgttttgctc aaggaagagg tcgctggatt tctggtatca accaattctc agttggcatg
    35461 gggaatggtg aaggaggagg ctataatggc gaaaataccg cattttgggc gaactggggt
    35521 tacagttgga actctcctgg ccccaatgct tggtatgtaa caacatcagg aaatatgtat
    35581 tgccgaaacg gagcagattt ccatgggaag gttgactttt cgaattcatc gagagcaaac
    35641 ttttatggga atactacttt ttctcgttct cctgtgtttt caaatggtat cgaacttgga
    35701 agtaaagacg tctttggtga tggttggaat cccaaaggcg gaaggaatgc ggttgtttgg
    35761 tggaatcagg tcggtagtgg tagcgtgaag tattggatgg aacaaaaatc agacagacgc
    35821 ctaaaagaga acatcacaga tacagctgtg aaagccttgg acaaaatcaa cagattaaga
    35881 atggttgcat ttgatttcat cgaaagtaag aaacatgagg agattggtct aatagctcaa
    35941 gaggctgaaa ccatcgttcc aagaattgtt tcacgagatc ctgagaatcc agatggctat
    36001 ctacatatag actataccgc tttagttcct tacttaatca aggccattca agaattaaat
    36061 caaaaaatag aaaaaatgga gaaaacaata gcatgaataa caacatgttg accaatatcg
    36121 cacttaaagc aattcaggag cttgctcttg aaaatagaaa acgaacagat agattggaga
    36181 acttagaaaa tgaacgcaga acagcttaac caagccttac aaatgacaat tagtgaaatg
    36241 tcaacaactt caacaaattc gatgattaca agtaatgtct tgagtattca gttggatgag
    36301 caaagggaag agaatcaaag acttcaagca cgagtggatg agctggaagc tctgcttgat
    36361 gaacaaacta aaccagcaga caaaggagaa tagacatggc aaaaacaatt caaaacacag
    36421 ataacttgct agaccttaca aaaatcacag aaccatttga tcttgcgagt gctttgcgct
    36481 acatgaaaga aagtggagag ttcattcgtt gcaagaatgt aaacgatgac ttctatatgt
    36541 atcgtgatgt tcaaaaacgt cctgtgttcg taaatggccg tcgccaattc aaggatgttg
    36601 aaaccgtttg ggcgttcaac cagtggggtg gtacaatcac aacaatcaac gtagccattc
    36661 tgttgaatca ggaattctat atcatgaaat ttgatgcaga gggcaatcct gactggacgg
    36721 atccaacggt agaacctaaa gaataggagg ttgtatgcca attgaagaag ctgaaaaaat
    36781 cgctcaaagt caggtagctt gggcgatttt gtttatcttg cttttcttta ttatcattcg
    36841 atatcttatc aagacttcgg acaagcgaga gaagaagatt atggatttgc acgagcaatc
    36901 aaaggccgac tctaatagac gagaagagcg tttgatgact catctagaaa agaccactac
    36961 agaattaacc acaatcactc acgcggtcgg agacattcaa aaagaaatgg ttcgcatgaa
    37021 cgaccgcatg gaagaaatcg aaaaaggaga ataacaaatg caacaaatta ctgaaatcat
    37081 tactaatgga gcaatcagca tcctagtcat tttggcaggg gttgtagtta gggcagtcaa
    37141 ggaatacctc gtcaaaaaag gtggagaaaa gactatcaag attgttgaaa tcttggccaa
    37201 gaacgcagtt aatgccgtgg agcaggtagc tgctgaaact ggctacaagg gagatgaaaa
    37261 actggcacag gctcgcgcta aaattcgtgc tgagcttaca aaatacaata ttagcatgac
    37321 tgacaaagac ttagacacct tcgtagagtc agcagtgaag cagatgaatg acgcatggaa
    37381 aggacgatag ggaatggata tcgatagaaa cagactacgt acaggcttgc cacaggttgg
    37441 ggtgcagcct tatcgacaag tacatgctca ctcaacaggc aaccgcaact caaccgtaca
    37501 gaatgaagcg gattatcact ggcggaaaga cccagaatta ggttttttct cgcacgttgt
    37561 tgggaacggt cgcatcatgc aggtaggacc tgtgaacaac ggaagttggg atgttggggg
    37621 cggttggaat gctgagacct atgcagcggt tgaactgatt gaaagccatt caactaaaga
    37681 agagttcatg acggactacc gcctctatat cgaattgcta cgcaatctag cggacgaagc
    37741 aggcttgccg aagactcttg atacagacga cttggcaggt atcaagacgc atgaatactg
    37801 taccaataac caaccaaaca accactcaga ccatgtggat ccatatccat atcttgcaag
    37861 ttggggcatt agccgtgaac agtttaagca agacatcgaa aacggcttga gcgctgcaac
    37921 aggctggcag aaaaatggca ctggctactg gtacgtacac tcagacggct cttatccaaa
    37981 agataagttt gagaaaatca acggtacctg gtattatttc gatggctcag gctatatgct
    38041 ttcagaccgc tggaagaagc acacagacgg taattggtac tactttgacc aatcaggcga
    38101 aatggccaca ggctggaaga aaatcgctga gaagtggtac tattttgatg tagaaggtgc
    38161 catgaagaca ggctgggtca agtacaagaa cacttggtac tacttagacg ctaaagaagg
    38221 cgccatggta tcaaatgctt ttatccagtc agcagacgga acaggctggt actacctcaa
    38281 accagacgga acactggcag acaagccaga gttcacagta gagccagatg gcttgattac
    38341 agttaaataa atagaaagga aactttctaa attgttcttt caccgcaggc tcaggcttgc
    38401 ggtttttttg tttgctcaaa aggggcaaaa aaggggcaaa agtgtcgtaa atctctgtaa
    38461 aatgatgtaa aaacatttat ttaaaagctc aaaatatagc tgttttaaaa ggtattgtaa
    38521 gatatagtaa aacgatgtaa aggtattttt aaatgcggat gaattataaa accacgaatc
    38581 ctatgtgact cgtggttctt ttttataaac tggtagagtg ttttggttgt actttttgtt
    38641 cagggtcaat gtagttgata gcgttgttga cagcagttgg agcttctccg aggcctgtcg
    38701 caatcagatc aatttttccg tcatagtagc agcagtcacc gatagcatag atacctgctt
    38761 ggctggattc ctgtttgctg ttgacgataa tcttgtgacg gttgaggtcg agcccccagt
    38821 ttttaaggtt accgacagaa gatttgaaac catagttgac aaagaggtgg tctaggtcaa
    38881 tggtttcagt ttcatcagat ttgacttttg tgatttcaag tttatcaagc gtttttccat
    38941 ctccaaggag ttggctaggg gtgaatggtg tcttgatggt tacagatgat tcttgcaagg
    39001 cttgaacact gtgttccaag gcacggaaat tatctctgcg gtgaacaagg gtagttggtg
    39061 cgattttttc aaaagccaaa gtccaatcca cagccgaatc tcccccacca agaatcgtca
    39121 ctttcttacc agcgtattgc tgaatgttag aaacgtggta gtggatattt tcatagccct
    39181 caaccccttc aagttccagc ggacgtggtt tgaaggcacc gccccccata gcgatgataa
    39241 ctgttttagt caggtgactt cctttagaag ttgtgatggc aaattcttct tcttgtttgt
    39301 caatctcaag aaccgtttca ttgagatgaa taggggtatc aaatccattt agctgttcaa
    39361 tcaagcggtt agtcaactct tctccagtca ggtttgggaa gcctggtacg tctaggattt
    39421 ccttttcagg gtagagaata gcaggttgtc cacctagctg gggaagagag tcgatgattt
    39481 gaaccttggc ttggcgtagg tgggcataaa aggctgcaaa aagcccgaca ggaccaccac
    39541 ccacaatggt aatatcatag agttgagaca tagtttctcc tttgtttttt ctagtcagtt
    39601 tattttatca tatttttgtt caatataaaa tggactagga tagggaaaaa aatgccagaa
    39661 aatggtataa taggacgaaa gtgttttgat agggaggata tcagggatga attttcaaca
    39721 attatccaat ctgcaatatt ggaccagttt gtttgcaagt ccatggacga tagctatcaa
    39781 tctgattgat attttgattg ttgcttatat tttataccat tttacaaaaa ctattgcagg
    39841 aaccaagatt atgattttgg tacgtggagt tttggtgttt attttagctc aaatccttgc
    39901 aaatatgatt ggtttgacta cgatttcttg gttaatcaat caaattatta cttatggggt
    39961 tattgcggcg gttgttattt tctctccaga gattcggact ggtttggaac gtttgggaag
    40021 agcgacagat ttcttttcca atgcccctat tagtgctgag gaacagatga ttcgtgcctt
    40081 tgttaagtct gttgaataca tgagtcctcg taaaatcggg gccttggttg ctattcagcg
    40141 tgtacgtacc ttgcaggagt atatttcgac aggaattccc ttggatgcta agatttctgc
    40201 agaacttctc attaacattt ttattcccaa cactccccta catgatggtg cggtgattat
    40261 caaagaagaa cgtatcgctg tgacgtctgc ctatctgccc ttgacaaaaa acacagggat
    40321 ttccaaggaa tttgggacca gacatcgggc ggctatcggt ttatcagaag tctcagatgc
    40381 cttgactttt gtcgtatcag aggaaacggg aggaatttcg ataacctata atggaaggtt
    40441 taagcacaac ctaacacttg atgaatttga aacagaatta cgtgaaatct tacttccaaa
    40501 agaggaagtg ggtcttagtt ttaaagaacg attgctagga ggatggaaac atgaaaaaaa
    40561 atagtttata tatcatatcc tcactctttt ttgcttgtgt tttatttgtc tatgctacgg
    40621 cgacgaattt tcaaaacagt accagtgcta ggcaggtaaa aacggaaacc tatactaata
    40681 cagtaacaaa tgtccctatt gacatacgct ataatagtga taagtatttt attagcggtt
    40741 ttgcttcaga agtatcagtg gtcttgactg gtgcaaatcg cctatcgcta gctagtgaaa
    40801 tgcaagaaag tacacgtaaa ttcaaggtta ctgctgacct aacagatgcc ggtgttggaa
    40861 cgattgaagt tcctttgagc attgaagctt tacccaatgg gctgaccgct gtggcgactc
    40921 cgcaaaaaat tacagtcaag attggtaaga aggctcagaa ggataaggta aagattgtac
    40981 cagagattga ccctagtcaa attgatagtc gggtacaaat tgaaaatgtc atggtgtcag
    41041 ataaagaagt gtcgattacg agtgaccaag agacattgga tagaattgat aagattatcg
    41101 ctgtcttgcc aactagcgaa cgtataacag gtaattacag tggttcagta cctttgcagg
    41161 caatcgaccg caatggtgtt gtcttaccgg cagttatcac tccgtttgat acaataatga
    41221 aggtgactac aaaaccagta gcaccaagtt caagcacatc aaattcaagt acaagcagtt
    41281 catcggagac atcttcgtca acgaaagcaa ctagttcaaa aacgaattaa aaatagaaaa
    41341 ggattttata acaatgggta aatattttgg gactgatgga gtccgtggag aagctaacct
    41401 agaactaaca ccagaattag cctttaaact aggacgtttt ggaggctatg ttcttagtca
    41461 acatgaaacg gaagcgccga aagtctttgt aggacgtgac acacgtattt caggggaatt
    41521 gctggaatcg gccttggtgg caggtctcct ttcagtaggg attcacgtat acaaacttgg
    41581 tgtccttgca acaccagcag tagcttactt ggttgaaact gaaggagcaa gtgccggtgt
    41641 catgatttct gctagccaca acccagccct tgataacgga atcaagttct ttggcggtga
    41701 tggcttcaaa ctagatgatg aaaaagaagc agaaattgaa gccttgctag atgctgagga
    41761 agacactctt cctcgtccaa gtgcagaagg cttaggaatt ttggtagatt atccagaagg
    41821 cttgcgtaag tatgaaggat accttgtttc aactggaact cctcttgatg gaatgaaggt
    41881 tgccttggat acagctaatg gagcagcttc taccagtgcc cgtcaaatct ttgcagacct
    41941 tggtgcccaa ttgacggtta tcggggaaac accagacggt cttaacatca accttaatgt
    42001 tggttcaaca catccaaaag cccttcaaga agtggtcaaa gaaagtgggt cagctattgg
    42061 tttggccttt gatggagaca gtgaccgctt gattgctgtt gatgagaatg gtgacatcgt
    42121 cgatggtgac aagattatgt acatcatcgg aaaatacctt tctgaaaaag gacaattggc
    42181 taaaaataca attgtgacaa ctgtgatgtc taaccttggt ttccacaagg ccttgaatcg
    42241 cgaaggtatt aacaaggcag ttactgcagt tggtgaccgc tacgttgttg aagaaatgag
    42301 aaaatcaggc tacaaccttg gtggtgaaca gtctggtcac gttatcttga tggattacaa
    42361 taccacaggt gatggtcaat tatcagcagt tcaattgact aaaatcatga agaaaactgg
    42421 taagagctta tcagagttgg cggcagaagt aacgatttat ccacaaaaat tagttaatat
    42481 ccgagtggaa aacgtcatga aggaaaaggc catggaagtg ccagctatca aggccatcat
    42541 cgagaagatg gaagaagaaa tggcggggaa cggccgtatc cttgttcgtc caagtggaac
    42601 agagcccctc ttgcgtgtta tggcagaagc gcctacaaca gaagaagtgg actactatgt
    42661 tgataccatc acagatgtag ttcgtgctga aattgggatt gactaaatcc tagaaaacta
    42721 gatgaaaata aaaaattatg gtacaatagc ttataatatt gtagccgatg gagaaagaca
    42781 tgaatcttaa acgtgaacaa gaatttgtta gtcagtatca ttttgatgcg cgtaattttg
    42841 aatgggaaaa tgaaaatgga gctcctgaaa ccaaggtgga tgtgaatttc caattgctcc
    42901 aacatgacca agaaaatcaa gtgacttcct tgattgtcat cttgagcttt atgattgtat
    42961 ttgataagtt tgtcattagt ggaacaatct cacaagtaaa ccatatcgat ggccgtattg
    43021 tcaatgaacc aagtgaattg aaccaagaag aggtggaaac cttggctcgt ccatgtttga
    43081 acatgctcaa tcgattgact tacgaagtga ctgaaattgc attagactta ccaggaatta
    43141 atttggagtt ctaatatgaa attagctgtt ttcacagatt cctctgctta tctcagtgaa
    43201 gagaccttgc aaagagaaga cttgtttgtc ttggatattc ctgttaatat tgatggtgag
    43261 gaatatgtcg aaggcatcaa tctgtctgct gaggaatttt accaaaaaat ggctcaggct
    43321 tctgaattgc ctaagaccag tcaaccaagt attgccaagt tagatgagat tttaacttcg
    43381 ctcaaagaac aaggctatac acatgccttg gggcttttcc tatcttctgg aatttcaggt
    43441 ttttaccaaa atatccagta tatggtcgat gactatgagg gcttaaccat tgcttttcca
    43501 gacactttga ttacaagtgc tcctctgggt atcatggttg aaagcgtctt taattggcgt
    43561 gatcagggcg atgattttgc cagcattcag gataagctag ccattcaaat cagccgtacg
    43621 tcagccttta tcatggtaga tgacttggat catttagtga aaggtggacg cctttcaaat
    43681 ggggctgcca ttttgggcaa tttgcttagc attaagccaa tcctttattt taacgatcaa
    43741 ggtgtgattg aagtttacga aaaagttcgt actgaaaaga aggccaccaa gcgcttaatt
    43801 gaaattatca aggaaacaac ggcttcaagt caatacaggg tcattgtcat tcatgggaat
    43861 gctcctgaaa aggctgaaga attgcgtcag catttgcttg attttggctt gggttcggat
    43921 gtttcacttg ctacatttgg tagtgtcatt ggaacgcacc taggagcagg aagtattgct
    43981 ctgggttata ttccagtgat ttagttggcg cttctaggct agttaagatg ccttgtatct
    44041 tagcaattga acacggacta cctgcctctt gaaaaaagat gtctgtcttg cctttcgtgg
    44101 aaagtcagtg ccatttccta ttttttatgg gtggctaaga tcctttgtat cttgataatt
    44161 gaacacgcct ggaaccctgt gtgaaaaaga tagttcttcc aaggagtaga cacttcttga
    44221 tcagaattcc tattttcact ttgtgttctt acaggctttg tatcttagac aggagtagag
    44281 atgagtattc gagtaattat tgccggtttt aagggaaaga tgggtcaagc tgcttgccag
    44341 atggtcttga ctgattcaga tttggacttg gtagcagttt tggatccttt tgagtctgag
    44401 tcagaatggc agggtattcc tgttttcaag gataaggctg atttagctgg ttttgaagcg
    44461 gatgtctggg tagactttac tactccagct gttgcctacg aaaatacacg ttttgctctt
    44521 gaaaatggct ttgctccagt agttggaacg actggtttca cgagtgaaga aattgcagag
    44581 ctaaaagaat tttctcgtgc ccaagacttg ggtggcctga ttgcccctaa ctttgccttg
    44641 ggtgctgtct tactcatgca atttgcgacg caggctgcca aatatttccc aaatgtggag
    44701 attattgagc tccatcatga caagaaaaag gatgctccga gtggaacagc cattaaaaca
    44761 gctgagttga tggcagaggt tcgagagtca attcagcaag gtgcagcaga tgaggaagag
    44821 ctgattgctg gtgctcgtgg tgctgacttt gatggtatgc gcatccactc agttcgtttg
    44881 ccaggcttgg tagctcatca ggaagtcatc tttggcaatc agggagaagg gttgaccctc
    44941 cgtcatgact cctatgatcg catctccttc atgacaggag tcaatttggg aattaaagaa
    45001 gttgtcaagc gtcatgagct tgtctatgga ttagaacact tattatgaga ttaacgcaaa
    45061 tgccttctga atttcagaag gctttaccag tattagaaaa aattaaagaa gcaggctttg
    45121 aggcttattt tgttgggggc tctgttcgag atgcccttct ccatagccct atccacgatg
    45181 tggatattgc gacgtcttct tatccagaag agaccaagca gatttttccg cgaacagccg
    45241 atatcggaat cgagcatgga accgtcttgg tcttagatgg ggatgaggag tatgaggtaa
    45301 caacctttcg gacagaggat gtctatgtgg actatcgcag acccagtgcg gtttcctttg
    45361 tgcgctcgct agaagaagac ctcaaacgcc gtgatttcac agtcaacgcc tttgccttgg
    45421 atgagacagg agaaatcgtt gacttgttcc atggtttaga agatttggaa aagcaagtct
    45481 tgcgagcagt tggagtggct agtgagcgtt tcaacgaaga tgctttacgg attatgcgtg
    45541 gtttccgttt tcaggctagt cttggttttg cacttgagcc agaaacattt aaagctatga
    45601 agaccttgac gccgcttttg gagaaaattt ctgtagagcg taccttcgtc gagtttgata
    45661 aactcttgct ggctccattt tggagaaggg gcttggcttc catgattgag agtcaagctt
    45721 atgactatct ccctgatatg gcatctagcc gggacaagct caacagactg tttgatttgg
    45781 agactgattt tacttttgaa tcctctgaac aagcctggtc ggctttacta tgggctttgg
    45841 agattgaaaa tgcgcagtca tttttgaaat tttggaagac ctcacgccag tttgccaagc
    45901 aagttcagga tttgctgatt attttggctc tgcgtgaaaa tggagaattg agcaagcgag
    45961 attgttatcg ctttgacata gatttgcttt tacaggctga aaatcttcgt caagctcaag
    46021 gaaaagaagt caacccacaa gccatcacag aaaaatacca aagcttgact atccatgaca
    46081 agaaagagat tcagattaat ggcggtattt tgatcaagga atatggctat cagccaggcc
    46141 cagacttggg agagatttta acagagattg agtttgccat tgtcgatgga gaattggaga
    46201 ataatcgtga agccatccat gcttacctga gggagaaaaa atgagtgatt ttatcgttga
    46261 aaaactaagt aaatccgttg gtgacaagac cgtttttagg gatatttcct ttattatcca
    46321 tgacttagac agaattggtt taatcggtgt caatggaact ggcaagacca cccttttgga
    46381 cgtcctttct ggtgtttctg gatttgatgg ggatgtcagt cctttttcag ctaaaaatga
    46441 ttaccagatt ggttacttga ctcaggatcc tgattttgat gatagaaaga cagttttgga
    46501 tacggttcta tctagtgaac tcaaggaaat ccagctcatt cgtgagtatg aattgattat
    46561 gctcgactat agtgaggaca agcaggcgcg tttggaacgt gtcatggcag agatggactc
    46621 tctccaagct tgggaaatcg agagtcaggt caagaccgtt cttagcaaat tgggcattca
    46681 agacttatct actcctgttg gggaattgtc aggtggtctg agaagacggg tacagttggc
    46741 acaagtctta cttggcaacc acgacctctt gcttttggat gagccgacca accatctgga
    46801 tattgcgatt attgagtggc tgaccctctt tttgaaaaat tctaagaaga ccgtcctttt
    46861 tatcactcac gatcgttatt tcttagacgc tttgtccaca cggattttcg agttggagcg
    46921 agcaggcttg acagaatatc agggcaatta ccaggactat gttcgcctta aggcagaaca
    46981 ggacgagcgc gatgcagctc tacttcataa aaaagaacaa ctctacaaac aagaactggc
    47041 ctggatgcgc agacaaccgc aggcgcgtgc gaccaagcag caggctcgta tcaatcgttt
    47101 ccacaacttg aagaaagaac tttcaggtgg cgttgctgag acagacttaa ctatgaactt
    47161 tgaaaccagt cggattggga agaaagtcat cgagtttcag gatgtttcct ttgcctatga
    47221 aaacaagccc attttgcaag attttaatct cttggtacag gtcaaagatc gtatcggaat
    47281 tgttggggac aacggtgttg gaaaatcaac cttactcaac ttaattgcag gaagtcttga
    47341 gtcaactaaa ggacaagttg tgattgggga aactgttcgc atcgcctatt tctctcagca
    47401 aattgagggc ttagatgaaa gcaagagagt tatcaattac ctgcaggaag tggcagaaga
    47461 ggtcaagacc agtggcggtt ctacgacttc gattgcagag ttgctggagc agttcctttt
    47521 cccacgttcg acgcatggaa ccttgattga gaaattgtca gggggtgaga aaaaacgtct
    47581 ttatctcctc aaactgcttt tggaaaaacc aaatgttctt cttttagacg agccaaccaa
    47641 tgacctagat attgcaactt tgacagtctt agagaatttc ttgcaaggtt ttgcaggtcc
    47701 cgttttaaca gtcagtcacg accgctattt cttggataag gtagcgacca agattctcgc
    47761 ttttgaggat ggcaagattc gtcctttctt tggtcattac accgactatc ttgatgaaaa
    47821 aacttttgaa acagatatgg ccaatcaagt gcaaaaggcc gaaaaggaaa aagtggtcaa
    47881 ggttcgagaa gacaagaaac gcatgaccta ccaagaaaag caggagtggg caagtattga
    47941 aggtgatatt gaagccttgg aaaaacgtat cgctgctatt gaagaggaaa tgcaggctaa
    48001 cggctctgac tttggtaagc tggctactct ccaaaaagaa ttggatgaga aaaatgaagc
    48061 actccttgaa aaatacgaac gctatgagta tctcagtgaa tttgatagtt aagactattc
    48121 gaaaatcaaa gtgcaaacta tgtcggcttc acattgccga ctcaagtaca gcctgtgacc
    48181 ggcttcctaa tttgcacttt gatttttatt gagtataaaa gaatagaaaa atcccgtctc
    48241 atgaaaggat ttctctattc ttttttggtt tcttgatgaa agatattttg ccaggtttcg
    48301 tggcgacgcc agatactggt atggatgata ccatctaact catagcagat gagtttggtt
    48361 ttttgactga tggaagtaat ttgaatgtcc ttgatagcag aatttaactc ttttttggtc
    48421 ttataagcct ctttatccat ctgctctcca tcttggcgaa tatagacaaa atcatcagtc
    48481 aagagttctt ctagttggtt tccttggtca atcaattgtt cacgcataag caatttttta
    48541 tagacattgt ctaaaatttc atcctcaggg ataggtgctg gttcgatatg gacatcggta
    48601 tcaaagacgc caaaacgctc ctccagcata gactcgacct gatccgcgat ttcatggctt
    48661 tcaaaaacag acaagtcagg attcatctct agtgtaatat ccaggtagat gttgctaccg
    48721 taggtgcgac ctctttgcga tttgaccttg ctgattttgg gaatttccat gatagccttt
    48781 tggtagtcct cgagcaggcg gtcgtcaaag ccatctgaaa gactaaagga agattcgatg
    48841 aagatatcat aggcagtctt caagataaag aaagtgatga tgatagcaac cagtttatcc
    48901 acaatcggat aattgaaact actagctagg atggcaatgg cggttccaag tgaggtaaca
    48961 gcgtcagaaa gattgtcctt agcagctgcc ttcagcgcct tggagttgga tttcttactg
    49021 aggcgagtat tgtagagata gaccacaaac ataatcgctg cagaaatgat tcctagagtt
    49081 gcaccaagag gatcaatgac cgtttcttcc cgactgagaa tcttttgaat ggtatctctt
    49141 agaacatcga aaccgacata gaacatgatg atagaagtga tcaagcttgc caaatcttca
    49201 atcttccaat gaccaaaacg gtggtcacgg tctgcaggct ggcgcgccat ccgaatcccg
    49261 attaagaggg ccacatttcc aatgatgtcc gatacgttat taaaaccatc ggccaccaaa
    49321 ctggatgaat gaaggagatg accagctgct aatttggctg cagacaagat caaataggtc
    49381 gaaatactga taatggctcc acgctcagct aacttgagat ttgagataga ttgcttcatt
    49441 caacttcctt tctagtcaca tagcattcta ccattatact ggttttcaag gaaaaattca
    49501 aataaggcag tttttactct ttgaaaactc cccaaaattt ggtataatgg tactactcaa
    49561 gggagtagct ggcagaaacc tgtgatagtg tcgtcattcc gaattttatg ctgaaaagta
    49621 tgctttccgg ccctatctta aacagcgaga cttgttatga ttaacaggtc tctttttgtt
    49681 tgtcgtgaaa agatatgaca gaaagagagt ctgttttgca cacaatgtca aggaggagac
    49741 acatgtcaaa agaacaaaaa cgccaagcgt tttatactca gagccctgaa gaggtcttgc
    49801 aggctgtgga tgcgaccgag caaggtttgt catcaagtga ggcggaaaag cgccttgccg
    49861 aatttgggca caatgaactc gaagaaggcg agaaacgatc aatcttggtc aaattcatcg
    49921 agcaatttaa ggatttgatg attatcatcc tagttgcggc agcaatcttg tcagtcgtga
    49981 cttctggtgg ggaagatatc gcagatgcca ttatcatcct agctgtggtt atcatcaacg
    50041 ctgcctttgg tgtttaccaa gaaggaaaag ctgaagaagc tattgaagcc ctcaaatcca
    50101 tgtctagtcc agttgcccgc gttcttcgtg atggacacat ggcagagatt gactctaaag
    50161 aattggtacc aggcgatatc gttgcccttg aagcaggtga cgtggtacca gcggacctac
    50221 gtttgataga agccaactct cttaaaattg aagaagcagc cttgacaggt gaatctgtac
    50281 cagtcgaaaa agacttgtca gtcgagcttg cgacagatgc tggtattggt gaccgtgtca
    50341 acatggcctt ccaaaactca aacgtaacct atggtcgtgg gatgggtgtt gttgtcaata
    50401 caggtatgta cactgaagtt ggtcatattg ctggtatgct tcaagatgcg gatgagactg
    50461 atacaccact caaacaaaac ttgaacaacc tttctaaggt cttgacctat gctatcttgg
    50521 tcattgccct tgttactttt gtagtgggtg tcttcattca agggaaaaat ccacttggtg
    50581 agttgttgac ttctgttgcc cttgccgttg cagccattcc agaaggactt cctgctatcg
    50641 ttaccatcgt tctttctctt ggtactcaag ttttggccaa acgacattcc atcgttcgta
    50701 agttgccagc agttgaaaca cttggttcaa ctgaaatcat cgcttctgat aagactggta
    50761 cgctgactat gaacaagatg acagtcgaaa aagtcttcta cgatgcggtt ctacatgact
    50821 cagctgatga tattgaacta ggtcttgaaa tgccactact tcgttcagtt ctcttggcca
    50881 atgatacgaa aatcgatgtg gaaggtaact tgattggtga cccaaccgaa acagccttta
    50941 tccaatatgc cttggacaag ggctatgatg tcaaaggttt cttagagaaa tatcctcgtg
    51001 tagctgaatt gccatttgac tctgaccgta agctcatgtc aacagttcac ccattgccag
    51061 atggtcgttt ccttgtagca gtcaagggtg cgccagacca actcttaaaa cgttgtcttc
    51121 ttcgtgataa ggctagggat attgctccga ttgatgagaa ggttacaaat ctcattcata
    51181 caaacaactc tgaaatggct catcaagcct tgcgtgtcct tgcaggtgct tataagatta
    51241 ttgacagcat tccagaaaat ctcacttctg aagagcttga aaatgattta atttttactg
    51301 gtttgattgg gatgattgac cctgaacgtc ctgaagccgc tgaggctgtt cgtgtggcta
    51361 aggaagcggg aatccgtcca attatgatta caggtgacca ccaagacaca gcggaagcca
    51421 ttgccaaacg tttgggaatc attgacgcaa acgatacaga aggtcacatt ttaactggtg
    51481 ctgaactcaa tgaactgtca gatgaagaat ttgaaaaagt cgttggtcaa tactctgttt
    51541 atgcccgtgt gtctccagaa cacaaggttc gtatcgtcaa ggcttggcaa aaacaaggta
    51601 aagtcgttgc catgacaggt gacggtgtca atgacgcgcc agctctgaaa acagccgata
    51661 tcggtatcgg tatgggaatc actggtacag agatttctaa gggggcttct gatatgattc
    51721 ttgcagatga taactttgcg actattatcg tcgcagtgga agaaggacgt aaggtcttct
    51781 caaacattca aaagactatt cagtacctac tttctgctaa tactgctgaa gtattaacca
    51841 tcttcctatc aaccttgttt ggttgggatg tcttacagcc ggttcatctt ttgtggatca
    51901 acttggtaac ggataccttc ccagctatcg ctcttggtgt tgaacctgcg gaacctggtg
    51961 tcatgaatca taaaccacgt ggacgcaagg caagcttctt ctcaggtggt gttttgagtt
    52021 ctatcattta tcaaggtgta ctccaagcag ctcttgttat gagtgtttat ggccttgcga
    52081 ttgcttaccc agttcatgtg ggtgacaatc atgctattca tgcagatgcc ctaacgatgg
    52141 cttttgcaac ccttggtttg attcagctct tccatgccta caatgtcaag tctgtctacc
    52201 aatccatctt gacagttggc ccattcaagt ctaagacctt taactggtcc atcttggtat
    52261 cctttatcct tctcatggca acaatcgttg tagaaccgct tgaaggaatc ttccacgtaa
    52321 ccaaactaga cttgtcacaa tggggaattg ttatggctgg aagcttctca atgattatca
    52381 tcgtcgaaat cgttaagttt attcaacgca aactcggttt tgacaagaat gcgatttaag
    52441 ggaggtttcg gatgtcagca tattaattac cgaccgtatg gcaggatgaa gctagtaatc
    52501 aaggagcttt tacggggcta aacagaccaa cagtaggtgc ccgtttcgaa caaaacttgc
    52561 caaaaggaga acaagctttt cagctttatt cactgggaac accaaatggt gtgaaggtta
    52621 ctatcttatt ggaagaatta ctagaagctg gttttaagga agcggcttac gacttgtata
    52681 agattgctat catggatggg gatcaattcg gatcagactt tgtgaagctc aatccaaatt
    52741 ccaagattcc agccttattg gaccagtcag gtactgaaga tgtaagagtc tttgagtctg
    52801 ctcatattct tctttacctt gctgagaaat ttggagcctt tttaccaagt aatcctgtgg
    52861 aaaaggtaga agttttgaat tggctattct ggcaagcagg tgcagcacct tttctaggtg
    52921 ggggatttgg acatttcttc aattatgctc ctgaaaaatt ggaatatcct attaaccgtt
    52981 ttacaatgga agtgaaacgc cagttggatt tattggataa ggaattggct cagaaacctt
    53041 atattgcagg caatgactat acgattgcag atattgctat ctggtcttgg tatggacagt
    53101 tagttcaagg aaatctttac caaggttctg caaaattctt ggatgcctca agttatcaaa
    53161 atctagtaaa atgggcagaa aaaattgcca atcgtccagc tgttaagcgt ggcttggaag
    53221 taacttatac agaaattaaa tagcaaaaaa gtcatcttcg gatggctttt tgctacagtt
    53281 gagggaaagg gtttgcagtt atcggctttt gtgctataat tgctataata tagtaaaaag
    53341 caagaggagt gtgaggaagt ggaaaagaaa attgtgaagg atatcttatt tttatctcaa
    53401 gtgtctcagc cggcaagtca ggaggacctt tatcttgcca gagatttgca ggatacactc
    53461 ttagcaaatc gtgatacctg tgttggtcta gctgccaata tgattggggt gcagaagcgc
    53521 gtgattatct ttaatcttgg cttggttccc gtggtcatgt ttaacccagt gcttctgtcc
    53581 tttgaaggat cctatgaggc agaagaaggc tgtttgtcct tggtaggtgt gagatcaact
    53641 aagtgttatg aaaccataag ggttgcctat cgtgacagca agtggcagga acagaccatt
    53701 accttgacag gcttcccagc tcagatttgc cagcatgagc tggatcactt ggaaggacga
    53761 atcatttagg aggaaagcaa atgaaacgaa tagtctttga acttattttt atcgcaacga
    53821 cctggtatat ctttttaccg ccccttaacc tgaccagctg ggaatttctc ttcttcctct
    53881 gtgggcattt gttagttgtg gcaatcttat ttggctttgg caaggggata aaccttgtca
    53941 aaacggttca tgtgcgccac ggtaaggcgg aagctgcctt aaatcttgag ggtttcaaaa
    54001 tcaatcggtt agggaaaatt ctgttagctt cgattggagg aattcttctc ttggcagctt
    54061 tggtttcctt ggtaacttct agcatgtttc aggctaaaaa ttatgccaat gtagtcacgg
    54121 ttacggaaaa agactttact gaatttccta agagtgacac cagtaaggtt cctatcctag
    54181 atagaagtac tgctgaaaaa attggagacc gctacttggg ttccctaacc gataaggtgt
    54241 cgcaatacgt agcggcagat acctataccc aattgacaat tgatgggaaa ccttatcggg
    54301 tcacaccact agaatatgca gaccctatca aatggtttaa caatcaagcc aagggaatcg
    54361 gtgagtatat caaggtggac atggtaactg gaaatgcgga tttggtggac ttgaagacac
    54421 caatcaagta ttcagactcg gagtatttta accgtgatgt caaacgtcac ctgcgcttga
    54481 agtacccgac caaaatcttt aaaactccat cttttgaggt ggacgatgag ggcaatcctt
    54541 tctatgtagc aacggtttac caaaagcaat ttggacttgc tgttcctcgt cctgcttcag
    54601 tcattatctt ggatgctaca aatggagaaa ccaaggaata cagcttatca gatgttccag
    54661 aatgggtgga caggatctat ccagcagagg aaaccattga gcaaattaac tacaacggca
    54721 agtacaagga cggtttcttg aatgccatga tttccaagaa aaacgtgacc cagactacca
    54781 agggctataa ttacttgtct atcggtaatg acatctatct ctacacaggt gtgacgtcgg
    54841 ctaatgcgga tgagagtaat cttggtttca tccttgaaaa tatgcgaaca ggagaaatca
    54901 ctaagtatag cttggcttct gcgacagaag aatcagcccg tgaatcagca gaaggtgctg
    54961 ttcaggagaa atcctacaaa gcaaccttcc caatcctcat caacctcaat gacaagcctc
    55021 tctacatcat gggcttaaag gacaatgctg gcttggtcaa agagtacgcc ctggtagacg
    55081 cagtcgagta ccaaaatgtt atcgttgcta ctacagtgga agagatgctc agcaagtatg
    55141 ccaataaaaa cgaccttgaa attgacaatg caacgacaga aagcatcaag ggagtagtag
    55201 cagacctcaa atcagctgtt atcaagggag acactgtcta cttctttaaa gttgatggca
    55261 agatatacaa ggtcaaggct tcagtatccg atgaccttcc ttaccttgaa aatggtaaaa
    55321 ccttcgaagg tcaagtagga aaagacaatt atctcaagac ctttaagcta cggtaaaaat
    55381 aggttttttc agaaagtata tgttataata gagtaaatta agactaatgg aggacaaaga
    55441 aatgggtttt tatttgatgg ttgcttcaat gctactcgga ctgctcgctt tgaagatagg
    55501 tttttctcag ttcaaggaga aaaaagataa attcttatct atcttaacaa gtttagctgg
    55561 cacagccctt gttctcgtgg ctgtctggtt aggatggccc aaataaatag aaaaacctcg
    55621 gtcaaaccga ggtttttcaa atgaatttct tggttgtggc taaaaaatat gctacactat
    55681 caatatgaaa attttaatcc caacagcaaa agaaatgaac acagacttgc caagtatcga
    55741 ggcaattcct ttaaaaccag aaagtcaggc cgtgcttgat gccttggctc tctattctgc
    55801 cagtcaattg gagagtttct acaaggtatc agctgagaaa gcggcggaag aatttcaaaa
    55861 tatccaagct ttgaaaaggc aaactgctca acactatcca gccttgaaac tttttgatgg
    55921 gcttatgtac cgcaacatta agagagataa gctgaccgag gcggaacaag attatcttga
    55981 aaatcatgtc ttcattacct cggctttgta cggtgttgtt ccagccttgt cacccatggc
    56041 tcctcaccgt ttggattttt tgatgaaatt aaaagtcgct ggtaagactt tgaagagcca
    56101 ttggaaggca gtctatgatg aagctctgaa gaaggaagaa gtgattttct ctctcttgtc
    56161 atcagagttt gagactgtat tttctaagga aatcagagca aagatggtga ccttcaaatt
    56221 catggaggat agagggggtc agctgaagat tcactcaact atctccaaga aggcgcgcgg
    56281 ggcctttcta acagctttaa tagaaaatca agtacaaact gtgggggaag cacgtcgctt
    56341 gaactttgct ggatttgttt accgagaaga tttgtcacaa ccacagggat tggtttttgt
    56401 aaaggaagta tagaaatcag atattttgta tttggcaata tgtatcatca gctattttag
    56461 tttataaatg agaaaaaagc gaacaagcta gtcttctgag tgtcagaaaa gtagtcttgt
    56521 tcgcttttat tatgttatac tcttcgaaaa tctaaaatct cttcaagcca cgtcagcgtc
    56581 gccttaccgt atatatgtta ctgacttcgt cagttctatc tacaacctca aagcagtgct
    56641 ttgagtagct tgcggctagc ttcctagttt gctctttgat tttcattgag tattagttta
    56701 taaatgagaa aaaagcgaac aagctaatct tctgagtgtc agaaaagcag tcttgttcgc
    56761 ttttattatg ttagtttagt tacttgatag cattgaccaa gtcttgagct tgtttgtcaa
    56821 gtgagtttag gacagtttct tcaagaacca atttcccgtc tgcccaggca gagtcattaa
    56881 cacgtgcagc agtgaaatcg ccaacgactt gtgtacggat aaatggcaag aggtctttgt
    56941 agatagcgaa gagttgatcg tgccctgcat tggctacaga tgatacggtg acaaacttgt
    57001 cttgaagggc agaagcacca cgtgtatcag acaagtcaag ggcacgagat agccagtcca
    57061 gcaagttctt cactgtacca gggatagaga agttgtagac tggagagaaa atccagatag
    57121 catccgcaac gagaactgct tcacgagcag cagctacagc tggatgtgtt ggaacttcca
    57181 aatcttggct gaagagagga agggctgaat aatcaaggta gctaacttcc gctttaccag
    57241 caagtgcttt ctcagcttcg agcgccattt ggtggttgaa agaaccttga cgtagtgatc
    57301 cgacgataaa taatactttt ttagacatga tgtgtctcct ttttcttcaa atttcgagaa
    57361 ctctctcgtt gttataaaat atattacaac aaaacaaaag aacttgcaag aaatttgctc
    57421 agagtttatt tgaaaattgt aaaatttcgg aaactctagt catttgtgac tgaaaaagta
    57481 agaactgttt cactttcaaa atcggttttg tttgaaggga gaaatagaat tggttgctca
    57541 aagcacagct ttgaggttgc agataaagct gacgtggttt gaagagattt tcgaagagta
    57601 ttcttatatt cacttgtaat aaagcttaat agatggtaaa atagacgagt gaaaaaaaga
    57661 caaaacaaag caaaaaataa tctactgtgg cagtatggtc tagggatgac gattttgttt
    57721 gtggttatca gtgcttcctt tctgtatatg gtttctctta gcatgaaacc ctatcaaaca
    57781 gctaaaagtg aaggagaaaa attagctcag cagtatgcag gattagagca ggccgatcag
    57841 gttgatttat acaatggctt ggaatcttat tacagcgttc ttggtcgtaa taaacagcaa
    57901 gaagcacttg ctgttctgat tggaaaagat gatcataaga tttacgttta tcagctaaat
    57961 cagggtgttt cacaagaaaa agcagaaacg gtttctaagg aaaagggagc tggcgaaatt
    58021 gacaagatta tctttggtcg ttatcaagat aagccaatct gggaagtcaa gtcaggatct
    58081 gatttttatc tagtagattt tgaaacagga gcattggtca acaaggaggg cctatgaaat
    58141 tatccaaccg tgttttagaa atggaagaaa gtgtgacttt ggctgctgga gcccgtgcca
    58201 aagcactgaa ggctgagggc agagatattt tgtctctaac cttgggtgag ccagatttca
    58261 ctactcccaa aaatatccaa gatgccgcca ttgcatcgat tcgagatggg cgtgcttctt
    58321 tttatacagt aacctcaggt ctgccagagc ttaaggcggc ggtcaatagc tactttgagc
    58381 gcttttacgg ctattctgta gcgtcaaatc aagtgacagt cgctgcggga gccaaatatt
    58441 ctctctatac cttctttatg gctgtggtca atccaggtga tgaagtcatc atcccaaccc
    58501 catactgggt tagctatgga gatcaggtca agatggcaga aggcgttccc gtctttgttt
    58561 ctgctaagga agacaatcac tttaaggtga ccgtggagca gttagaagca gctcgcactg
    58621 acaagaccaa ggttttggtg ctgaattcgc catctaatcc cacaggtatg atttacaccc
    58681 gtgaggaact cttggcaatt ggaaactggg ctgtagaaaa tgatattctc atcctagcag
    58741 atgatatcta tggccgcttg gtttataatg gtcatgagtt cacaccgatt tctagcctat
    58801 cggaagcgat tcgcaagcaa acagtggtca tcaatggtgt gtctaaaacc tatgccatga
    58861 ctggttggcg gattggttat gccgttggag aagcagacat tatcgctgcc atgtccaaga
    58921 ttgcaggtca aacaacttcg aatccgtcag cagtagccca atatgcagca gttgaggctc
    58981 tatcaggtga gcaagatact gtagaaagca tgcgtcaggc ctttgaggaa cgtcttaata
    59041 ccatctatcc cctccttgca gaggtgccag gatttgaagt ggtcaaacca caaggggcct
    59101 tctatctctt cccaaatgtc aaaaaggcca tggagatgaa aggctacacg gatgtgacag
    59161 actttacaac tgttatctta gaagaagccg aagtggcctt ggtaacagga gctggatttg
    59221 gagcaccaga aaatgtccgc ctcagctatg cgacagacct agacacgctt aaagaagcag
    59281 tcgaacgctt gaaagcattt atgggtagtg agaataattg atcattttga gattaaggta
    59341 aaggatttac aaattttaga aggattttat aggagttttc tcgctccttt ggactataaa
    59401 ttggctttta agactagttc tctaattagt tttctatccc cgaacagccc tcatcctggt
    59461 ggtgattttt ggctgactca gggaacacaa gatcctgttc attttgcttt tttagcagaa
    59521 aataaggaag aagttcaggc ttgttatgag gctggcttag aggcaggtgg gcgagacaat
    59581 ggggctcctg gttatcgcag tgagcatccg atttactatg ctgcttttat gattgacctg
    59641 gatgggaaca atatagaggt ggtttgccat aaagaataaa aaagaaaagg aaaaataatg
    59701 acaaaacgtg taacgattat tgacgtaaaa gactatgttg gtcaggaagt gacgattggc
    59761 gcttgggttg ccaacaaatc aggaaaaggg aaaatcgcct tcttacaatt gcgtgatgga
    59821 acagccttct ttcaaggtgt ggcttttaaa ccaaactttg tcgaaaaatt tggtgaagaa
    59881 gtgggacttg agaagtttga tgttatcaaa cgcttgagcc aagaaacgtc tgtttatgtg
    59941 acaggtattg tcaaagagga cgaacgttct aaatttggct atgagttgga catcacagac
    60001 atcgaagtga tcggtgaatc tcaagactac ccaatcacac caaaagaaca cggaacagac
    60061 tttttgatgg ataaccgtca cttgtggcta cgctctcgta agcaagtagc tgtgttgcaa
    60121 atccgtaacg ctattatcta tgcaacttat gagttctttg acaagaacgg ttttatgaag
    60181 tttgacagcc caattctttc aggaaatgcg gcagaagatt ctacagaact ctttgaaact
    60241 gactacttcg gaacgccagc ctacttgagc caatcaggtc agctttacct agaagcaggg
    60301 gctatggctc ttggtcgtgt ctttgacttt ggtccagttt ttcgtgctga aaaatcaaaa
    60361 acacgccgtc acttgactga gttctggatg atggatgctg agtactcata cttgacacat
    60421 gatgagtcgc ttgacttgca agaagcttat gtgaaagctc ttctacaagg tgttcttgac
    60481 cgcgcgcctc aagccttgga aaccttggaa cgtgatacag aactcttgaa acgctacatt
    60541 gcagagccat tcaaacgtat cacttatgat caagccattg acctcttgca agagcatgaa
    60601 aatgatgaag acgctgatta cgagcatctt gagcatggtg atgactttgg ttcgccacat
    60661 gaaacttgga tctcaaatca ctttggtgta ccaacttttg tcatgaacta tccagcagcc
    60721 atcaaggcct tctacatgaa accagttcct ggaaatccag agcgcgtgct ttgtgcagac
    60781 ttgcttgctc cagaaggtta cggagaaatc atcggtgggt ctatgcgtga ggaagactac
    60841 gatgcccttg tcgctaagat ggatgaactt ggcatggatc gtacagagta cgaattttac
    60901 cttgaccttc gtaaatacgg tacagttcca cacggtggat ttggtatcgg tatcgaacgt
    60961 atggtaacct tcgcagcagg aacaaaacac atccgtgaag ccattccatt cccacgtatg
    61021 ttgcatcgta tcaaaccgta agaataggaa aacgtccatg tggtgttttt tcgaattcca
    61081 gagtttttac ttgcgtaaaa aatttttttc tagtataata gttcattgtg agcgaacctc
    61141 acttacccct tgcaaagtct tggggtcatt agaccaaaag gaggaacata tcaatggcta
    61201 aatacgaaat tctttatatc attcgtccaa acattgaaga agaagctaaa aacgctttgg
    61261 tagcacgttt tgactctatt ttgactgaca acggtgcaac tgttgttgaa tcaaaaactt
    61321 gggaaaaacg tcgtcttgca tacgaaatca aagatttccg tgaaggactt taccacatcg
    61381 ttaacgttga agcaaacgac gacgtagctc ttaaagagtt tgaccgtctt tcaaaaatca
    61441 acgctgacat tcttcgtcac atgatcgtca aaattgacgc gtaagaaggt aaactatgat
    61501 taacaatgtt gtacttgtgg ggcgtatgac acgtgacgct gagttgcgtt ataccccatc
    61561 aaatgtagca gttgcgactt ttactcttgc agtaaaccgt acatttaaga gtcaaaatgg
    61621 cgaacgtgag gctgatttta tcaatgtcgt tatgtggcgc caacaggctg aaaaccttgc
    61681 taattgggct aaaaaaggct cacttatcgg ggtgacaggc cgtatccaga ctcgtagtta
    61741 cgataaccag caaggacaac gtgtctacgt gacagaggtc gtggctgaga atttccaaat
    61801 gttggaaagc cgtagtgtgc gtgaaggtca cacagttggt gcctattctg caccaactgc
    61861 aaactattca gcgcctacaa attcagtacc agacttttca cgtgatgaaa atccatttgg
    61921 agcaactaat ccattggata tttcagatga tgatttacca ttctaatgga caactaaaat
    61981 tataaaggag aaaaaacatg gctcaacaac gtcgtggcgg attcaaacgc cgtaaaaaag
    62041 ttgattacat cgcagcaaac aaaattgaat atgttgatta caaagatact gagcttctta
    62101 gccgtttcgt ttcagaacgt gggaaaatcc ttcctcgtcg tgtaacagga acttcagcta
    62161 aaaaccaacg taaagtaaca acagctatca aacgcgctcg cgtaatggct ttgatgcctt
    62221 tcgtaaacga agattaaaga aaagaggctc tttgtcaact gtagtgggtt gaagtcagct
    62281 aagctcgaga aaggacaaat ttcgtccttt cttttttgat gttcaaagcg atagaaatcc
    62341 gttttttgaa gttttcaaag tttcgaaaac caaaggcatt gcgcttgata agtttgatga
    62401 gattattggt cgcttccagt ttggcattag aatagtgtag ttgaagggcg ttaacaattt
    62461 tctctttatc tttaaggaag gttttaaaaa cagtctgaaa aagaggat
//
LOCUS       DAVKDZ010000004        59111 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000004 DAVKDZ010000000
VERSION     DAVKDZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 59111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59111
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.004"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(324..2582)
                     /locus_tag="VWZ30_000235"
     CDS             complement(324..2582)
                     /locus_tag="VWZ30_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEU9334052.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTSRGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGDENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(2608..4125)
                     /gene="malQ"
                     /locus_tag="VWZ30_000236"
     CDS             complement(2608..4125)
                     /gene="malQ"
                     /locus_tag="VWZ30_000236"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU9334053.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEHFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            4675..5946
                     /locus_tag="VWZ30_000237"
     CDS             4675..5946
                     /locus_tag="VWZ30_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9334054.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKITLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            6057..7349
                     /locus_tag="VWZ30_000238"
     CDS             6057..7349
                     /locus_tag="VWZ30_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9334055.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            7351..8193
                     /locus_tag="VWZ30_000239"
     CDS             7351..8193
                     /locus_tag="VWZ30_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9334056.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            8454..9254
                     /locus_tag="VWZ30_000240"
     CDS             8454..9254
                     /locus_tag="VWZ30_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEU9334057.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFGLNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTDKVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTKELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFKTFKECYHFILNCL
                     GLPTLITLILGLFGQNMSNLITVQNILFVLYLVTIFYKTHFRDPDYHK"
     gene            9264..10250
                     /locus_tag="VWZ30_000241"
     CDS             9264..10250
                     /locus_tag="VWZ30_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9334058.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            10386..10466
                     /locus_tag="VWZ30_000242"
                     /pseudo
     CDS             10386..10466
                     /locus_tag="VWZ30_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(10542..11483)
                     /locus_tag="VWZ30_000243"
     CDS             complement(10542..11483)
                     /locus_tag="VWZ30_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9334059.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(11461..13224)
                     /gene="aspS"
                     /locus_tag="VWZ30_000244"
     CDS             complement(11461..13224)
                     /gene="aspS"
                     /locus_tag="VWZ30_000244"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU9334060.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            complement(13415..13495)
                     /locus_tag="VWZ30_000245"
                     /pseudo
     CDS             complement(13415..13495)
                     /locus_tag="VWZ30_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(13531..13761)
                     /locus_tag="VWZ30_000246"
     CDS             complement(13531..13761)
                     /locus_tag="VWZ30_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334061.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(13779..14420)
                     /locus_tag="VWZ30_000247"
     CDS             complement(13779..14420)
                     /locus_tag="VWZ30_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9334062.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATSSLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKIGFNRFIWLPALIWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYVSRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(14432..15166)
                     /locus_tag="VWZ30_000248"
     CDS             complement(14432..15166)
                     /locus_tag="VWZ30_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEU9334063.1"
                     /translation="MKKRRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKILIP
                     WISAICLVLVFISILLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHINPQTFFLSFLLFVVFLVAQKYLFKTIAIVRQFDLE
                     FFATPKDILNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            complement(15169..15366)
                     /locus_tag="VWZ30_000249"
     CDS             complement(15169..15366)
                     /locus_tag="VWZ30_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334064.1"
                     /translation="MNKYKVIYYVVVIALSVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            complement(15366..15572)
                     /locus_tag="VWZ30_000250"
     CDS             complement(15366..15572)
                     /locus_tag="VWZ30_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9334065.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(15582..15896)
                     /locus_tag="VWZ30_000251"
     CDS             complement(15582..15896)
                     /locus_tag="VWZ30_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334066.1"
                     /translation="MWVLVFILFMIFFYSNNSKKIKKLEKKIKKLERKEKGNTEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(16356..17681)
                     /locus_tag="VWZ30_000252"
     CDS             complement(16356..17681)
                     /locus_tag="VWZ30_000252"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012948.1,HMM:TIGR01168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEU9334067.1"
                     /translation="MRARHERRNRYSIRKFSVGVASVVVATFFCAGGGGQVVQAADGA
                     RPQGVSSTLDSYRKTLHDLLGRLSLTEGEKEKYRNQIDTATELDKLVELEGTLDNLQN
                     KEIEEAVEKAKVTLPQYMDTRLKELDQTGSGFPDLLKKLQNIIDEYREQLDGLPNKKA
                     VEDLVEQAKEEMDGYIAEYQKTLTAGQDDNNGAGEQAPQAGDNNGAGEQVPQAPQAGD
                     NNGAGEQVPQAPQAPQAGDNNGAGEQAPQAPQAGDQTFTGTVGSVNVTVLFDKPVNAE
                     KVTVKEITEKDLVDKISRQAGGGSIRLFDLSLTKDGKETHVNEERTVRLALEGLGENV
                     QVYHVKPDGQLELLDSKVEDGHVIFRINHFSLFAIKTMSTKSNQETPSNQATPSTQAK
                     STPKETTKDATSKKTLPNTGTADSTALLAAAASTAILGLGLAGRRRKED"
     gene            17991..19517
                     /locus_tag="VWZ30_000253"
                     /pseudo
     CDS             17991..19517
                     /locus_tag="VWZ30_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(19716..19796)
                     /locus_tag="VWZ30_000254"
                     /pseudo
     CDS             complement(19716..19796)
                     /locus_tag="VWZ30_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(19787..21076)
                     /locus_tag="VWZ30_000255"
     CDS             complement(19787..21076)
                     /locus_tag="VWZ30_000255"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU9334068.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVT
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGEVANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(21154..21822)
                     /locus_tag="VWZ30_000256"
     CDS             complement(21154..21822)
                     /locus_tag="VWZ30_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9334069.1"
                     /translation="MSLSMSSADGESLKNEKLSIKYQFLTIYRNLRLVFLKSGQKNFG
                     FMLFAVLLINALGGSLGGIYNIFFLSHSLLNFSYTEALFISQVGALVAVIISSLTGND
                     YFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFFTLYVSGKVQPKISAMLMKN
                     LAPEVLARTSNFLGLLFTLSIPVGTVCFSLVAVWNIQLTWMLFVGISLLAIFLTILNL
                     KNDI"
     gene            complement(22108..22401)
                     /locus_tag="VWZ30_000257"
     CDS             complement(22108..22401)
                     /locus_tag="VWZ30_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334070.1"
                     /translation="MKLLFRNQAYRLLTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALSCSAS"
     gene            complement(22592..23443)
                     /gene="rgg"
                     /locus_tag="VWZ30_000258"
     CDS             complement(22592..23443)
                     /gene="rgg"
                     /locus_tag="VWZ30_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEU9334071.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFLPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSIDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFINAYYFK
                     RVIEEHFYKENETYFRIVYLWAEDLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            23567..23908
                     /locus_tag="VWZ30_000259"
     CDS             23567..23908
                     /locus_tag="VWZ30_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9334072.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIEDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            23966..24046
                     /locus_tag="VWZ30_000260"
                     /pseudo
     CDS             23966..24046
                     /locus_tag="VWZ30_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(24162..25865)
                     /gene="ilvD"
                     /locus_tag="VWZ30_000261"
     CDS             complement(24162..25865)
                     /gene="ilvD"
                     /locus_tag="VWZ30_000261"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU9334073.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETPIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(26098..27030)
                     /locus_tag="VWZ30_000262"
     CDS             complement(26098..27030)
                     /locus_tag="VWZ30_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU9334074.1"
                     /translation="MVRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATNNLEK
                     NFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPRSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPEAVYQDGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(27024..27884)
                     /locus_tag="VWZ30_000263"
     CDS             complement(27024..27884)
                     /locus_tag="VWZ30_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9334075.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFVARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETEW"
     gene            complement(27888..29234)
                     /locus_tag="VWZ30_000264"
     CDS             complement(27888..29234)
                     /locus_tag="VWZ30_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9334076.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(29247..29531)
                     /locus_tag="VWZ30_000265"
     CDS             complement(29247..29531)
                     /locus_tag="VWZ30_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9334077.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKICEEKGLI"
     gene            complement(29535..31565)
                     /locus_tag="VWZ30_000266"
     CDS             complement(29535..31565)
                     /locus_tag="VWZ30_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU9334078.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTDDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTPKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            31757..32764
                     /locus_tag="VWZ30_000267"
     CDS             31757..32764
                     /locus_tag="VWZ30_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9334079.1"
                     /translation="MTEKLINSKPNGVFALILIELTLVLGVFIFIMGVGSQNIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKFPFLGAKSSNDNDVNLEISKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQSSAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            32767..32967
                     /locus_tag="VWZ30_000268"
     CDS             32767..32967
                     /locus_tag="VWZ30_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9334080.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYASETIDEPFEIDI"
     gene            33119..33301
                     /gene="rpmF"
                     /locus_tag="VWZ30_000269"
     CDS             33119..33301
                     /gene="rpmF"
                     /locus_tag="VWZ30_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU9334081.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            33317..33466
                     /gene="rpmG"
                     /locus_tag="VWZ30_000270"
     CDS             33317..33466
                     /gene="rpmG"
                     /locus_tag="VWZ30_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9334082.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(33596..33979)
                     /locus_tag="VWZ30_000271"
     CDS             complement(33596..33979)
                     /locus_tag="VWZ30_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334083.1"
                     /translation="MKTKNIEIRRITFSKFEFNTHNTGEVEEDGRASINLNFPDREEG
                     QENQLILIKMEISYPDSQYRVIADIDARYSVLASDLPEDISQDDEFQQALITPMIEKF
                     RLYVGMFSEGAYGAVLIPNLNFNQD"
     gene            complement(33981..34568)
                     /locus_tag="VWZ30_000272"
     CDS             complement(33981..34568)
                     /locus_tag="VWZ30_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HEU9334084.1"
                     /translation="MMKDKEYYLGLNYPILIEKIYDEGELLFTASIKELPGLIVYGES
                     LGEVYDEIELAKADWIEANLEWGRKIKEPFENSLEEYSGKITLRLPKTLHRDVKERSE
                     IEGVSLNQTIVQLINDGLFKQTQNAVEQLTEQFKDMTKKLSQTIAEKVSAPEQHIHQT
                     IIIKNQSEKIQPYKEQNITNNRLQDNKVVSWSIGG"
     gene            complement(34555..34812)
                     /locus_tag="VWZ30_000273"
     CDS             complement(34555..34812)
                     /locus_tag="VWZ30_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicA family toxin-antitoxin system"
                     /protein_id="HEU9334085.1"
                     /translation="MGKEKELQKIKNNPKSVTPERLESLCKKYGFMWKQGTNHSITSH
                     ALLEESYPIPRHKPIKPVYMKKVIKMIEKVIELEEGLNDER"
     gene            complement(34930..35250)
                     /locus_tag="VWZ30_000274"
     CDS             complement(34930..35250)
                     /locus_tag="VWZ30_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9334086.1"
                     /translation="MYHTEISMFLRGEASQLAKYNKKSGKPYKDIDDLVDFGRPIHIV
                     PHMFRHSFVSVMAEKNVSLNVIREFVGHSEDSREIEKIYLHVMQKGKDKIEQAMIDLA
                     EIIF"
     gene            35406..35612
                     /locus_tag="VWZ30_000275"
                     /pseudo
     CDS             35406..35612
                     /locus_tag="VWZ30_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(35775..36044)
                     /locus_tag="VWZ30_000276"
     CDS             complement(35775..36044)
                     /locus_tag="VWZ30_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334087.1"
                     /translation="MPKTKTLAELADVILWSFDFAIDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREI"
     gene            complement(36431..38311)
                     /locus_tag="VWZ30_000277"
     CDS             complement(36431..38311)
                     /locus_tag="VWZ30_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9334088.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVSVAQSDQASISIKGE
                     DGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEILALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSVEELQEIEAYAQQFDMTF
                     VPCIQTLPHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDSMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILDGVVNRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAGTIADIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(38305..39174)
                     /locus_tag="VWZ30_000278"
     CDS             complement(38305..39174)
                     /locus_tag="VWZ30_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9334089.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWHEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMTINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGIKKAVDNFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(39258..41894)
                     /locus_tag="VWZ30_000279"
     CDS             complement(39258..41894)
                     /locus_tag="VWZ30_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU9334090.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVTAFGRGVKQIGFDNQV
                     LEDEQFTSQFSKMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNRWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHNSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVESLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKSYEVLENTARYAKILLKHELTVPVSADEKLEEEQKGIIEFMKREAGRSE
                     ELTNIPLETEFTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPGASWENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASGELG
                     DWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGSVVA
                     TGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLERPY
                     PVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(42085..43352)
                     /locus_tag="VWZ30_000280"
                     /pseudo
     CDS             complement(42085..43352)
                     /locus_tag="VWZ30_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
     gene            43523..45619
                     /locus_tag="VWZ30_000281"
     CDS             43523..45619
                     /locus_tag="VWZ30_000281"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU9334091.1"
                     /translation="MKPLLEIIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTGQLGGDSLFHRQSSYDRDK
                     ASFQPHYLKLFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADKLTVDQIDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLVSSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKASAQADWENLLHRFDVIETGEANRTFFDHCLYRLFLFPQTF
                     YEINESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFQPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDFLKAE
                     QLTFTLSWLPSQPSTS"
     gene            complement(45857..46231)
                     /locus_tag="VWZ30_000282"
     CDS             complement(45857..46231)
                     /locus_tag="VWZ30_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface anchored protein"
                     /protein_id="HEU9334092.1"
                     /translation="MIIQESAKLDAETAKEATDLLADAKKVFADPKASQDEVDAMVKR
                     IEDFMAKVSPATDHTSPAKDQAAQTPAVAPATAQAANANQAAANARTVAKELPNTGTA
                     DSTVAMVVAAASALPWSCRPSS"
     gene            complement(46376..48064)
                     /locus_tag="VWZ30_000283"
     CDS             complement(46376..48064)
                     /locus_tag="VWZ30_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU9334093.1"
                     /translation="MVREIKPHSPLPSQVQLAYLEDELVAFIHFGPNTFYNQEWGTGQ
                     EDPERFNPTQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSIKVSPWRRG
                     KGDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPAY
                     GNGGKFAEVWMDGARGEGAQKVNYEFETWFETIRDLQGDCLIFSTEGTSIRWIGNERG
                     YAGDPLWQKVNPDKLGTEAELDYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQYPKSL
                     EELVEIYFHSVGRGTPLLLNIPPNKDGLFDAKDVERLYEFAAYRNELYKKDLALGAEI
                     SGSALSVDFACHHLTDGLKTSSGASDVGLPIQLELDLGSPKTFDVIELREDLKLGQRI
                     ATFYVQVEVDDVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLYK
                     TPGLSKKEVVQELAFAEKSLAVTKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAY
                     QDEIQVLQFQAGESKKDLSIPTLYFAADKILDFYLNLTVGGQLIDQAHILVETR"
     gene            complement(48066..51817)
                     /locus_tag="VWZ30_000284"
                     /pseudo
     CDS             complement(48066..51817)
                     /locus_tag="VWZ30_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250572.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
     gene            complement(52055..56503)
                     /locus_tag="VWZ30_000285"
     CDS             complement(52055..56503)
                     /locus_tag="VWZ30_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9334094.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMDAQPVQATE
                     TSSTLISSHYLVRDSSNKELSVDKVESPALSKKEDGSEPQSKKIVPQTASHFSSTEDF
                     VQSPQPSYAVEKIVEAPVSKQAPDEIVPIGTKEEIAGNPKVEQPKAENNSDYKPSPEE
                     GVLNATVEKPELLVTTEEIAFQTIEQEDASLAKGQTKVVQEGVVGERTIYTEVTIVNG
                     EKSSKVIENIVTKEPENKVIAVGTKEEVAPKPTQPVTPEPEEVKPVQPEKIEKKPIVE
                     NETETKPAAGIGQPRPGAEETPGEKQTPDKPEAEPKQPELASPAVGSGREEDQSPAEQ
                     KGEENQLENPVEGAKDAGESAPQEPQKQPEQTAPSPEVNPSQGNEPAPAVQPEPSAPQ
                     EQSDSQVQPTVPSPVTKEKVLDYKTVYTASPALNYKEQQVEVAGENGKEVTTTSYSFD
                     ESTGKIVENTSTKIEKHPVDRVVKVGNVEETTSTTKRGEQFVADESLDKGVKEVRNQG
                     QDEETTTIRVYKVNAQTGDLTEPEVSTKVAKEMQAKITAVGTKPTVQSQEIPFKTVYK
                     ASPDLSYNVQQEETPGEKGRTVSTTKYTVNQETGAISEETTIENIPAKDKIVKVGNVE
                     KIVSPIEITELRKDNPELPKGKEEVEDAGEQGETTVTKTYEVNPETGELTNPVEKTET
                     TKAMRQKVILVGTKEDKPHLLPINSELENAVNVTEATAEMRNVDLLTNEKLKAQLAPS
                     DIEINRDLFLKRKELRKTNPAITDEEVKEILRKEYLEKLSIKETLDATKTDLEASLKK
                     VAAHTLSILGDNQQNREKVKGDIEANKEKILLGLSYINRFYNIDFGDTNIRDILAYNP
                     SSFGKKDLTSLDWLTYLGSMSYDELRLTNSPKTFEKYFGKITDKPTLLEFLDYNRTTF
                     TNMDGDTWLKKATKAIVVEKPSKQKPDEKVELYTKLTTPPEKYGAENLQIESRRQQNV
                     AILLGLVNIKEPSVYAITNIATVTYGNIGTYMDTSLEKTNPVKYKEELEKVKALIELT
                     ATRQAAYVDTLYRITKEENRSKLVTNRVIVDTMKKYTADTSAGIGTTWSKESGPTADK
                     GVKDFMTPLGLYSPSQNVGAEANGVGVRYFIDRVLDDRGSATYSHEMTHLLDRTVLFN
                     NHGRRDGTAAEFYARGIFENSYTPEKDTYFNLNFVYDESKKNGFYNKTPDRFKTDADL
                     KSYMHGSFDVLYSLDYLEAEATKQLTAEDKTKYFKKITPIASKGPRATVTYTNSAVKA
                     THKSEKISEITLAEAEKLTDINSLIDNNILVNRYIINGFYATGDVKANGYYLVDMFDT
                     IYGVSQNDSGMSGDITFRKQAFELMAALGYYEGFVPYVSNQYKQVAESENKPLSDTYI
                     FNKILNGKSYAEFKKAQFKERVAKIDQLKPLTIQYEGQQISLTSQKLKELMQKAVQEE
                     LKQIKAGKTTARTYTFIETPVQKLKKAIYKAYLKDSDDFRQSIYNS"
     gene            complement(56821..57705)
                     /locus_tag="VWZ30_000286"
     CDS             complement(56821..57705)
                     /locus_tag="VWZ30_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9334095.1"
                     /translation="MIFLIVCVFLLLIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     MRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            57962..58819
                     /locus_tag="VWZ30_000287"
                     /pseudo
     CDS             57962..58819
                     /locus_tag="VWZ30_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520758.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
BASE COUNT        17238 a        13074 c        10898 g        17901 t
ORIGIN      
        1 actttatgta agcgaataca agagatatga ggtaaccaac tacgaaaaaa tataaggctg
       61 aagacagtaa aaaccagcat ttaaagcctt cactgtatct aggatttccc tatttttaga
      121 caagattgac ccaaatccac agcatttaaa aaagcgaatt tcaatcgaaa ctcgcttttt
      181 tacaaaatgt attagtataa atttgtatct tatactcaat gaaaatcaaa gagcaaacta
      241 ggaagctagc cgcaggttgc tcaaagcacc gctttgaggt tgcagataaa gctgacgtgg
      301 tttgaagaga ttttcgaaga gtattagttc aagtgccaga tgtcttcgtt atactgagcg
      361 attgtacggt cagatgagaa gaatcctgct ttagaaatgt taacgatgac tttatccaac
      421 catgcgtcac ggtcttcgta gtcagcaagc atttgctctt tgactttgat gtagtcttcc
      481 aagtcaagaa gagtcatgaa ccagtctttg ttgatcaatt cattgtaaag acgttccaag
      541 cgctctttgt ttccagctgc aagaactgca tcgctaacaa tgaagtcaac caatggtttg
      601 atagcttcac gagcgtagaa ttcgcttgat ttatatgctg cttttgcgta aaggtcgata
      661 acagtttctg aatcttcacc aaagatgtag atgttttcgt caccaaccaa ctcagcgatt
      721 tccacgttag ctccgtccat agtaccaagt gtcaaagctc cgttcaacat gaatttcatg
      781 ttaccagtac ctgaagcttc tttagaagca agtgagattt gttctgagat atcacatgct
      841 gggataagga agcttgctgc agtaacgttg tagttttcaa ccataactac ttgcaagtgt
      901 ggagctactg ctggatcgtt agcaataact tctgacatgc aaaggatcaa gtgaatgata
      961 tcttgagcga ttgtgtaggc tggagctgct ttaccaccaa agaagattgt gattggacga
     1021 gcagggatgt taccagcttt gatgtcaagg tatttgtgga tcacgtacaa agcgttcatt
     1081 tgttggcgtt tgtactcgtg aagacgtttg atttggatat caaagataga atttggattg
     1141 atttccacac cttggtgttc tttcaagtga cgagccaatt tacgtttgtt gtgagccttg
     1201 atgctttcca atttttcttt gacagctgct ttgtcttcat aagacaacag tttttcaagc
     1261 tcatctgctt catggtgcca accatctcca agaatctcat ccaagtagtg agacaatctt
     1321 gggttagcat gcataagcca acgacggaaa gtgataccgt ttgttttgtt gttgaacttt
     1381 tctgggtaaa ggtcgtagaa ggctttcaac tcagaatttt tcaagatttc agtatggagt
     1441 gctgcaaccc cgttaacact gtatccgtag tggatatcca tgtgagccat gtgaacacgt
     1501 ccgctctcat cgatgatttg aacagctgga tctttgtact ctgccttcac acgacggtcc
     1561 aattcttcga tgattggtac caagtgagga accacttctt gcaagaattc aagaggccat
     1621 ttttcaagcg cttcagcaag gattgtgtgg ttagtgtagg cagtcatgct acgaacaatt
     1681 gagattgctt cgtcaagatc gataccacgt gaagtcaaaa gacgaatcaa ttcaggaatc
     1741 accattgatg ggtgagtatc gttgatttgg acaactgcgt agtcagcaag gtcatgcaag
     1801 ttgcttcctt tttcgattgc ttcgtcgatg atcaattgcg caccgtttga aaccatgaag
     1861 tattgttgga agatacggag caattcacct tgacggtcac tatcatctgg gtaaaggaag
     1921 agagttaagt tgcgagcgat atctgtcttg tcaaagttga taccatcttt aataatagaa
     1981 gaatcaactg aatccaagtc aaacaaacgc aagcggtttt tagtcgctgt ttcataacca
     2041 gtaacatcaa tatcgtaaag agttgatgtc aaagtaaagt ctgcaaatgg tacttggtag
     2101 ctacggcttg agcgaaccaa ccagttttgc tctgtcaacc atgcatttgg aattgtttct
     2161 tgttggttgt ttttaagaac ttgttggaaa agaccaaagt ggtagttaag accaacaccg
     2221 tcaccattca aaccaagagt agcaattgag tcgataaagc aggcagccaa acgtcccaaa
     2281 ccaccattac caagagatgg ttccaattca acttcttcaa cttcgatcaa gtctttacct
     2341 gcagctgcaa gttctttttt aacatcgtcg taaagaccaa ggttaatcaa gttgtttgac
     2401 aagagtttac caatcaagaa ctcagctgag atgtagtaaa cttttttctt accagtgttg
     2461 actggttttt ggctgcttgc aagcttgctg tagttaagaa gagcaaggta aagctcttca
     2521 ttgctacatt ctgcaatggt tttattgtaa cgattttgta caaattcttg tagtgataac
     2581 atgtttagtt gtctcctgat tattgtctta tttctttaaa tctaccaaat tttcattaat
     2641 tcggcgataa attgttgtca agtcaagcaa accttcctcg acagctggtg tcaattgatc
     2701 ttcagtcata cgccaagacc agtttccacc aagggtagat gggaagttca tacgagctgc
     2761 ctcatccaat tctagtaaat cttgcatagt tgcaattgcc ataaagctaa ctgatgaaaa
     2821 tactgtacga agcatagcgt gtaccactgt ttcgtattct ttacggttcg tgtaacgagc
     2881 catgtactca cgagtcgcat catcaatctc attacggtac caaccaagaa ccgtattgtt
     2941 atcgtgtgtt cctgtgtaca taactgagtt agcaggtgcc aagtgtgggc tatcaatgct
     3001 ttcgtcttct gggttgaagg caaattgaag aatcttcatt cctgggaagc cagtacgttc
     3061 acgcaattcg atcacttcat ctgtcatgaa gccaaggtct tctgcgatga tgtttagctc
     3121 accaagttct tccttaacgg ctgcaaaaag cttgtagcct ggacctttca cccactcacc
     3181 aggtgctgct gtatcggaac cagcagggat ttcccagtaa gattcgaagc cacggaagtg
     3241 gtcgatacga acgatatcgt agattttgaa gctttcacgc aagcgttcaa tccaccattt
     3301 gtagccgtct ttgtccattg cttcccagtc atagattggg ttaccccaaa gctgaccagt
     3361 tacagaaaac tcatctggtg ggcatcctgc gatacaagta gccttaccat tgacatctgt
     3421 tttgaagaga tgtggatttg cccacatatc acttgaatct tccgctacgt agattggcat
     3481 gtccccaacg atttcaatgt ggttgtcgtt agcgtaagct ttcaatttca accattgttg
     3541 gaagaagaag tattgagtca cacggtggta aaccaacttg tctgccaatt gctcacgata
     3601 gctttcaagt gctgaagctt tacgagcacg agcatctgca tctggccatt cagtccaagc
     3661 aagattgtca aaatgctctt tgatagccat atactcagca aagagctcaa gccatgattg
     3721 gttgtcttga gcaaatttct caaaatcttt aacatctccg acttcaaaga aacgtttcac
     3781 cgctttttct aaaagaggac gacgtgcata gtagatttta gcatagtcaa cttcagacgc
     3841 atcgctacca aagtcaactc cttcaaggtc acttgcttcc aacaaacctt gctccaccaa
     3901 gatatctaaa tcgataaaat gagtgtttcc tgcgaaggct gagaaagatt ggtaaggaga
     3961 gtccccgtaa ctagttgctc ctaatggaag gatttgccag taacgttgtt ttgtacggac
     4021 caagaaatca acgaagtcgt aagcactttg accaaatgat ccgattccgt aagctcctgg
     4081 aagagaagag atgtgcatca acacaccact ttgacgtttt ttcataatag cacctcgtgt
     4141 gttaaaataa tggaacgttg cgtattttgc agacgcaaac gtttgcgtta cttataagta
     4201 tactcctttt caagcataat tgcaagcgtt tttaaaaaac ttttttgatt atttttaagg
     4261 agactaactc attttagcaa agataactta aaactatcta atcctaaagt ttgactctca
     4321 cgagacagtc tacaaagtac aaaacctcct tagggagctt gaatttatat gcaacaaagc
     4381 acaaacgtct atagaaaata gatagggatt agaaccaggg aggtagcccc tcctggtttc
     4441 cctctttaga cagattccat atatttttat taaaaatact gtattcttat ctatttcata
     4501 cttttcttct attaaaatgc aaaattatgc tatcaaatca agcttaaaaa ttttttaaaa
     4561 tttttacaaa aaatacttgc aaccgttttc tatttgtgct atactaagct cataaaggaa
     4621 aacgtttgcg tttccttatc aataaaactt gctattcttt gggaggaata cactatgtca
     4681 tctaaattta tgaagagcac tgcggtgctt gggactgtta cacttgctag cttgcttttg
     4741 gtagcttgcg gaagcaaaac tgctgataag cctgctgatt ctggttcatc tgaagtcaaa
     4801 gaactcactg tatatgtaga cgagggatat aagagctata ttgaagaggt tgctaaagct
     4861 tatgaaaaag aagctggagt aaaaatcact cttaaaactg gtgatgctct aggaggtctt
     4921 gataaacttt ctcttgacaa ccaatctggt aatgtccctg atgttatgat ggctccatac
     4981 gaccgtgtag gtagccttgg ttctgacgga caactttcag aagtgaaatt gagcgatggt
     5041 gctaaaacag acgacacaac taaatctctt gtaacagctg ctaatggtaa agtttacggt
     5101 gctcctgccg ttatcgagtc acttgttatg tactacaaca aagacttggt aaaagacgct
     5161 ccaaaaacat ttgctgactt ggaaaacctt gctaaagata gcaaatacgc attcgctggt
     5221 gaagatggta aaactactgc cttcctagct gactggacaa acttctacta tacatatgga
     5281 cttcttgccg gtaacggtgc ttacgtcttt ggccaaaacg gtaaagacgc taaagacatc
     5341 ggtcttgcaa acgacggttc tatcgtaggt atcaactacg ctaaatcttg gtacgaaaaa
     5401 tggcctaaag gtatgcaaga tacagaaggt gctggaaact taatccaaac tcaattccaa
     5461 gaaggtaaaa cagctgctat catcgacgga ccttggaaag ctcaagcctt taaagatgct
     5521 aaagtaaact acggagttgc aactatccca actcttccaa atggaaaaga atatgctgca
     5581 ttcggtggtg gtaaagcttg ggtcattcct caagccgtta agaaccttga agcttctcaa
     5641 aaatttgtag acttccttgt tgcaactgaa caacaaaaag tattatatga taagactaac
     5701 gaaatcccag ctaatactga ggctcgttca tacgctgaag gtaaaaacga tgagttgaca
     5761 acagctgtta tcaaacagtt caagaacact caaccactgc caaacatctc tcaaatgtct
     5821 gcagtttggg atccagcgaa aaatatgctc tttgatgctg taagtggtca aaaagatgct
     5881 aaaacagctg ctaacgatgc tgtaacattg atcaaagaaa caatcaaaca aaaatttggt
     5941 gaataaaaaa tttgttcaag gggggtggaa atcaaatccc cctttgaatt tatcaataga
     6001 gacacaaata atttagcttt cttataaaaa agtagtatcc tatgaaagga gttaatatgg
     6061 aaaagcaaca acctagtaaa gcagccctgc tgtctatcat tcctgggtta ggacagattt
     6121 acaataaaca aaaagccaaa ggttttatct tccttggtgt aaccatcgta tttgtccttt
     6181 acttcctagc acttgcaacc cctgaattga gcaacctcat cactcttggt gacaaaccag
     6241 gtcgtgataa ttccctcttt atgctgattc gtggtgcctt ccatctaatc tttgtaatcg
     6301 tttatgtact cttttatttc tcaaatatca aagatgcaca tacgattgca aaacgcatta
     6361 acaatggaat tccagttcca cgcacactca aagacatgat caaagggatt tatgaaaatg
     6421 gcttccctta cctcttgatc attccatctt atgttgccat gaccttcgcg attatcttcc
     6481 cagttatcgt aaccttgatg atcgccttta ccaactacga cttccaacac ttgccaccaa
     6541 acaagttgtt ggactgggtt ggtttgacca actttacaaa catttggagc ttgagtacct
     6601 tccgttctgc ctttggttct gttctttctt ggactatcat ttgggctttg gcagcttcta
     6661 ctttacaaat cgtaattggt atcttcacag ctatcattgc taaccaacca tttatcaaag
     6721 gaaaacgtat ctttggtgtt attttccttc ttccttgggc tgtcccagcc ttcatcacta
     6781 tcttgacatt ctcaaacatg tttaacgata gtgtcggtgc tatcaacact caagtattgc
     6841 caatcttggc taaattcctt cctttccttg atggagctct tattccttgg aaaacagacc
     6901 caacttggac taagattgcc ttgattatga tgcaaggttg gctcggattc ccatacatct
     6961 acgttctgac cttgggtatc ttgcaatcta ttcctaacga cctttacgaa gcagcttata
     7021 ttgacggtgc caacgcttgg caaaaattcc gcaacatcac tttcccaatg atcttggctg
     7081 ttgcggcacc tactttgatt agccaataca ccttcaactt taacaacttc tctatcatgt
     7141 acctcttcaa tggtggagga cctggtagtg tcggaggtgg agctggttca accgatatct
     7201 tgatctcatg gatctaccgt ttgacaacag gtacatctcc tcaatactca atggcggcag
     7261 ctgttacctt gatcatctct atcattgtca tttcaatctc tatgatcgca ttcaagaaac
     7321 tacacgcatt tgatatggag gacgtctaag atgaataact caattaaact caaacgtaga
     7381 ctgactcaaa gccttactta cctttacctg attggtctat caattgtaat tatctatcca
     7441 ctgttgatta ccattatgtc agcctttaaa gcaggtaacg tctcagcctt taaactagat
     7501 actaatatcg acctcaattt tgataacttt aaaggcctct tcactgaaac cttgtacggt
     7561 acttggtacc tcaacacttt gattatcgcc ttaattacca tggctgttca aacaagtatc
     7621 atcgtacttg ctggttatgc ttacagccgt tacaacttct tggctcgtaa acaaagtttg
     7681 gtcttcttct tgatcatcca aatggtgcca actatggccg ctttgacagc cttcttcgtt
     7741 atggcgctta tgttgaacgc ccttaaccac aactggttcc tcatcttcct ctacgttggt
     7801 ggtggtatcc cgatgaatgc ttggctcatg aaaggctact tcgatacagt gccaatgtct
     7861 ttagacgaat ctgcaaaact agacggtgca ggacacttcc gccgcttctg gcaaattgtt
     7921 cttccacttg ttcgcccaat ggttgccgta caagctctct gggccttcat gggacctttc
     7981 ggggactaca tcctctctag tttcttgctt cgtgagaaag aatactttac tgttgccgta
     8041 ggtctccaaa ccttcgttaa caatgcgaaa aacttgaaga ttgcctactt ctcagcaggt
     8101 gctatcctca tcgcccttcc aatctgtatt ctcttcttct tcctacaaaa gaactttgtt
     8161 tcaggactta caagtggtgg cgacaaggga taatttatcc ccaccaccct ttttcatttt
     8221 atactcttcg aaaatctctt caaaccacgt cagctttatc tccaacctca aagttgtgct
     8281 ttgagcaacc tgcggctagc ttcctagttt gcactttgat tttcattgac tattagcgat
     8341 tgtcactgta aataatatcc ttgtagcaag caatttaccc cctagacttg aaataaagcg
     8401 catttctcta tataataata ctcatataga aaacaccttt tagaaagata cctatgcttc
     8461 catatccatt ttcctatttt tcaagtattt gggggtttcg taagcccctg tccaaacgtt
     8521 tcgggctcaa ctggtttcaa cttctcttta ccagtatctt ccttatcagc ttgtctatgg
     8581 tacccattgc tatccaaaac agctcccagg agacctatcc gctagaaact tttatcgata
     8641 atgtctatga acctctgaca gataaggttg tccaggatct ctctgaacat gctacaattg
     8701 tcgatggcac attaacttat actggaacag ctagtcaagc cccttctgtt gtgattggtc
     8761 caagtcaaat caaggaatta cctaaggact tgcaactgca tttcgacaca aaagaactgg
     8821 tcatcagcaa ggaaagcaag gaattgaccc gtatctctta ccgagcgatt cagactgaga
     8881 gtttcaaaag taaagacagc ttgacccaag caatttctaa agactggtac caacagaatc
     8941 gtgtctatat cagtctcttc ctagttctcg gtgcgagctt cctctttggt ttgaatttct
     9001 ttatcgtctc tctaggggcg agtcttctcc tttatatcac taagaaatca cgcctctttt
     9061 catttaaaac ctttaaagag tgctaccatt ttatcttgaa ctgtttagga ttgccgactc
     9121 tgattacgct tattttggga ttgtttggcc aaaatatgtc aaacctcatc actgtacaaa
     9181 acattctttt tgtcctttat ctggtcacta tcttttataa aacgcatttc cgtgatccag
     9241 attaccataa ataggagatt tttatgcccg ttacgattaa agacgtggcc aaggctgctg
     9301 gtgtttcccc ttcaaccgta acccgtgtta ttcaaaataa atcaaccatt agcgacgaaa
     9361 ctaaaaaacg cgttcgcaag gcgatgaagg agctcaatta ccaccccaac ctcaatgctc
     9421 gtagcttggt aagcagctat actcaggtga ttggtttggt tcttcctgat gactcagacg
     9481 ccttctatca aaatcctttc ttcccatcag tcctacgcgg catctctcaa gtcgcatctg
     9541 aaaaccacta tgccattcag atagcaacag ggaaagatga gaaggagcgt ctcaacgcta
     9601 tttcacaaat ggtctacggc aagcgtgtag atgggctaat ctttctctat gcccaagaag
     9661 aagaccctct cgtaaaactc gtcgcagaag aacagttccc cttccttatc ctaggtaaat
     9721 ctctatctcc tttcatccca cttgtcgaca acgacaatgt tcaagctggt tttgatgcga
     9781 ctgaatattt catcaaaaaa ggctgcaaac gcattgcctt tatcggagga agtaaaaagc
     9841 tcttcgtgac caaagaccgt ttaacaggct atgaacaggc gcttaaacat tacaaactta
     9901 ccactgacaa caatcgcatc tactttgccg acgagtttct ggaagaaaag ggctataaat
     9961 ttagcaagcg attattcaag cacgatccac aaattgatgc tatcatcaca accgatagcc
    10021 tcctagctga aggtgtttgt aactatattg ccaaacacca gctggatgtc cctgttctca
    10081 gctttgactc ggttaatccc aagctcaact tggcagccta tgtcgatatc aatagtttag
    10141 agcttggtcg tgtttccctt gaaactattc tccagattat taatgataat aaaaacaata
    10201 aacaaatttg ttaccgtcaa ttgatcgccc acaaaattat cgaaaaataa gagactgggc
    10261 aaagagtcgt taaaagcaaa aacgcatact atcaggtatt gaaaaaactt gatactatgc
    10321 gttttattgt gggaagattt acttcctttt ctactgaaat tgagtctttt cccaagatct
    10381 ttttatactc aatgaaaatc aaagtgcaaa ctaggaagct agccgcaggt tgctcaaaac
    10441 actgttttga ggttgtggat agaactgacg aagtcagtaa ccatacctac ggcaaggcga
    10501 cgctgacgtg gtttgaagag attttcgaag agtattaatc actaattatc tatctcaaca
    10561 aatcttccta gaatatgaac attttccgag acagagacaa aggagcttgg atccacttgt
    10621 gtcataatct gtttaaattc attaaactct gcacgtgtaa tgacagtgat taaaactgcc
    10681 tttctctcgt gattataggt tccttctgca tcgtggatca tggttgctcc gcggtgcaat
    10741 tttttatgga ttttttcaat taccttctct ggatgatttg tcacaatcat ggcctgcata
    10801 cgcttttgct tagtaaagac tgcgtctgtc acacggctag agacaaagat ggtaatcata
    10861 gaataaagag cgtatttcca accaaaggtc aaacctgcta tcagcatgat agttccattt
    10921 accaagaaag aaatactacc gacattctta cccgttttct tgcgaatagt caggctgacg
    10981 atatccgtcc caccactgga gatattgttt cgaagagcaa aaccaatccc caaacccata
    11041 acaacacccc caaaaaggga attgataatg ggatcctccg tcaaggttgc cacagggaca
    11101 aactggataa agaaggaact catagatacc gtgataaagg taaagacggt gaacttatgg
    11161 ccaatctgat accaagctaa gaccatcaaa gggaagttaa tggcgtagaa gcttagcgaa
    11221 atcggaatat gaaaaccaaa ccagtgatta ctcaaggcag agataatctg tgccagacct
    11281 gttgcaccac tcgaatacac atgccctggt tggaaaaaga aattaactgc tactgctgat
    11341 aaaaaaccat agaccagaga ggccgaaatc ttctcatcat acttttctcg agagatactt
    11401 tgtaagacac gtaaaatttt tatctgataa gcaaagcggc gcagataata gcgccaccgc
    11461 ttaattcgtt ttgtttgttt catcttcttc tacttgtaag ctgagttcct ctagttgttt
    11521 gagagcgact gttgatggag cttgtgtcat tgggtcagtt gccttgttgt tcttaggaaa
    11581 ggcaatgact tcacggatat tttcttctcc agcaagcaac atgacaaagc gatcaagccc
    11641 gatagccaaa ccaccgtgtg gtgggaaacc atagtccatg gcttcaagaa ggaaaccaaa
    11701 ctggtcattg gcttcttcag ttgagaaacc aagagccttg aacatgcgtt cttgaaggtc
    11761 tttttggttg atacgaaggc taccaccacc aagctcataa ccgttcaaga cgatatcgta
    11821 agcaatggca cgaaccttag ccaaatcacc ttctaattcg tgagcagtct cttcctgtgg
    11881 aagtgtgaat ggatggtggg cgctcatgta gcggccttct tcttcagacc attcaaacat
    11941 cggccagtca accacccaaa ggaagttgaa cttatcatta tcaatcaagc caagctcttt
    12001 agcaatacgt ccacgaaggg cacccagtgt tgcattagcc acttcaagcg tatccgccac
    12061 aaagagaacc aagtccttat cttcaagagc aagcgctgtt gtcaattctt cttggatacc
    12121 agtcaagaac ttggcaactg gtccgtttaa ttctccatca accaccttga cccaagcaag
    12181 acctttggca ccatactgtt tggctacttc cgtcatcttg tcgatgtctt tacgtgaata
    12241 gttgtccgca gctcctttga ccacaatcgc ttttacagca ggtgcttctg aaaagacttt
    12301 aaagtctaca cctttgacca cttctgtcaa gtcctgaagc aacatgtcaa aacgagtatc
    12361 tggcttgtca gaaccgtaaa gagccatagc atcatcgtat ttcatacgag ggaacggtag
    12421 cgttacttcg atgccttttg tttccttcat cacgcgcgcg atcaagcctt ctgtaatatc
    12481 ttggatttct tgctcagtaa ggaaggacgt ttccaagtcg acctgagtaa attcaggctg
    12541 gcggtctcca cgcaagtcct cgtcacggaa acatttaacg atttggtagt aacggtcaaa
    12601 accagcattc atcaagagct gtttcgtgat ttgtggactt tgaggaagag cgtaaaaatg
    12661 ccccttatta acacgagacg gcactaaata atcacgcgcc ccttcaggcg ttgacttaga
    12721 aaggaatggt gtctccacgt cgataaactc caactcatcc aaatagttgc ggatagagtg
    12781 ggtcaccttg gcacgaagtt taagattttc caacatttct ggacgacgaa ggtcaaggta
    12841 acggtaacgc aaacgtgtat cgtcatttgc ctcaatgcca tccttaatct caaatggtgt
    12901 tgtcttagct gtgttaagca caataagagc tgtcacgttt aactcaaccg caccagttgg
    12961 caacttatca ttggcttgct cacgcgcagc gacctgacca gtcacctcaa taacaaattc
    13021 gctacgaagg ctttcagctg ttgccataac ctctgcagat actttttcag ggttgataac
    13081 caactgcatg attccttcac ggtcacgaag atcgataaag atcaaaccac caaggtcacg
    13141 acgacggcca acccatcctt tcaaggttat ttcttgtccg atgtgttcct cacgaacacg
    13201 accagcatac atactacgtt tcattatttc tctcctcttt tattctgtta ctattttacc
    13261 ataaaagcgc aggctcttca tgaaaatcat cagaaaagtt tgccagtctt taaaagtcag
    13321 gtgaaagccc taaaaattag cgctaatact cttcgaaaat ctcttcaaac cacgtcagcg
    13381 tcgccttacc gtaggtatgg ttactgactt cgtcagttct atccacaacc tcaaaacagt
    13441 gttttgagca acctgcggtt agcttcctag tttgctcttt gattttcatt gagtataata
    13501 caaaaatccg atgaacttca ccggactctc ttattttgaa tttttgcctg ctttacgctt
    13561 ttcagcaatt tcggctgcct ttcgaggcaa gacaatttcc gttatgtaag ccgtcccaaa
    13621 acgcagtaca cctgcaatag gagcaaagac aactgctaga tagttataga agaaatcgcc
    13681 tttgaaggca taagctagcg ctccaatgat gaaaaataga acaactgctt gaatcactgc
    13741 taataaaatt actcgtttca tgtgacctcc tgactctatt atagcatgag aatcatcaaa
    13801 aagccgacta aattattcaa agcgtgaaga gaaatactgt agaccagacc ttttctgcta
    13861 acgtaagcca aacccaaact aaaaccaagg ctaaaataga caaaaaattg ttgcacatca
    13921 cctggaaaat gaatcaaggc aaatagaaga ctagatacca gaagaaaaat cagggttcgt
    13981 ttactattgt cctgcttagg aaagagatag cgtgctaaca tccctctaaa aacaatctct
    14041 tccgtcaaag gagcaaaaat aaccacagca aaaaatgaga aaagtggttg agacaaggtc
    14101 aagtctgtcg ctatttgttg atttactgaa ggatcatctg gcaagaagaa ttgaacgacc
    14161 agagataaga accaaatcaa ggcaggaagc caaataaatc ggttaaagcc gatcttctca
    14221 atatgaacag gagctttctg ataccatttg taaatgccgt acacataaac tccagccaag
    14281 gccacataaa gcaaggtaac agcatagggt gaagccccta aggcaagcga agaggtcgcc
    14341 agcccctgaa taaagccata gataaagaaa aaggatagaa gggctagaag aaaccagcca
    14401 aggtttttaa gtagtttcat agataactcc tttatttgaa ataacgtttt accataggta
    14461 actgcatcac attgatataa acatggatgg ctcctacaag caagaaagct agtaactgaa
    14521 tctctcctgt caagaaagaa atgataataa gaaaaatata taaggctggt aagacatatt
    14581 ggtgtaattg gaataaaatt cgaaaactct gttccaaatt agcctgacgc tccccttcat
    14641 cataagaatt tatatagttc aagatatcct ttggtgtagc gaaaaattcc aaatcaaact
    14701 gacgaacaat cgcaatggtt ttaaaaagat atttttgagc gactaagaat accacaaaga
    14761 gtaagaaaga aaggaaaaat gtttgagggt ttatatgcaa tataatcacc tcacttaatg
    14821 aaataaaaat agccaatgga atcgctacac ctgtaatatt aaaagcaatg gttccaaact
    14881 caagattccg atacatttgc acataatagg tttcattcag atcatcatcc atttcctctt
    14941 gatacaagga gtgaaatttt ctgcttttct ttaggaaatt gaaagtcaaa agaatgctaa
    15001 tgaaaactaa tactaaacaa atagctgata tccatggtat caagattttt acatctaaaa
    15061 taatttcgtg agattcgaca cgcgctttaa acatccctac aaacatgccc aagaaacctc
    15121 ccaagacaat agacatcaaa aataacaatc tacgtctctt tttcatattc attctccttt
    15181 ttcacttgct agatttttgg atttcttttc aatccattca attactggga tgagagcaaa
    15241 gtagacccaa acaaattggt cgctttgata gggattaaac cagcttaggt ccatcccaat
    15301 cagtagaaat acgctgactg ataaagctat gaccactaca taataaatca ctttatactt
    15361 gttcatcact cgtcctcctc caagcgaaat accgattcga ctgtttcgtt gaaaatttga
    15421 gatattttca gggcaatgat aatggacggg gtgtactcat cccgttctag taggctaatg
    15481 gtctgtctgg aaactcctgc cagcttggct aggtcggttt gattgagacc atcgcgagct
    15541 ctaagctctt ttagacgatt ttttagttgc atgttacaca cctactctcc gtcaaattca
    15601 acggtttgaa tatcctcaat acgttgcaac ttgaattttt cttttcccgt attatctaca
    15661 cgtcgtagct ttacccattc ctcatcaaca tccacaactt cccagttatc tggcccaata
    15721 tacactcccg ttataattgg ttcctttcca atcatttctt gtaataatct cgacatttct
    15781 gtgtttcctt tctcttttcg ctcaagtttt ttgattttct tctctagttt cttgattttt
    15841 ttagaattat tagaataaaa gaaaatcata aatagtataa atactagtac ccacattata
    15901 actcctttct gcttcctatt tcttaacttg aattcattgt aacacctctt tttctttttg
    15961 taaaagaaaa agaggctggg acaaaaatca taaaaaggta tcctatcagg tgttcagaaa
    16021 ccttggtagg atgcctttta taatggaaag atttactgga ttttctcctc aaattgatac
    16081 tcaatgaaaa tcaaaaccta aactaggaag ctggctggat aggactgacc aagtcatcac
    16141 agacctagag caaggcgacg ctgacctgat ttgctccttg atttttgaag agtatagatt
    16201 cttaaaatgg atagataaga caaaaaaaac aaacaagacc gtattctgag tatcagaaaa
    16261 ctcgtcttgt tcgtttttta tatttaaggt tagactttta tcccaggttc ttagctctgt
    16321 taggcaaggc tagaggggac agagggtctt ttcccttaat cttctttacg acgacgtcct
    16381 gctagaccaa gtcctaagat agcagtgctt gctgctgcag ccagcaaggc tgttgaatca
    16441 gcagttcctg tatttggcaa tgttttcttg cttgtagcat ccttggtcgt ttctttagga
    16501 gtcgatttcg cttgagtact tggagttgct tggttagaag gtgtctcctg attagactta
    16561 gtagacatag tcttaatagc aaacaagctg aaatgattga ttctaaagat gacatgccca
    16621 tcttctactt tagaatcaag aagttcgagt tgaccatctg gtttcacatg gtagacttga
    16681 acattctctc caagaccttc aagcgcaaga cgaacagtcc gctcctcatt tacatgtgtt
    16741 tccttgccat cttttgttaa ggataggtca aataggcgaa tagatcctcc acctgcttgg
    16801 cgactgatct tatcaacaag atccttttca gtaatctcct tgacggtcac tttttctgca
    16861 ttaaccggct tgtcaaataa gacggtcaca tttacacttc caactgtgcc tgtaaaggtt
    16921 tggtcgcctg cttgcggtgc ttgcggtgct tgttcaccag ctccgttgtt gtcgcctgct
    16981 tgcggtgctt gaggtgcttg aggtacttgt tcaccagctc cattgttgtc gcctgcttgc
    17041 ggtgcttgag gtacttgttc accagctccg ttgttgtcgc ctgcttgagg tgcttgttca
    17101 ccagctccgt tgttgtcgtc ttggccagca gttaatgttt tttgatattc agcaatataa
    17161 ccatccattt cctctttggc ttgttctacc aagtcttcca ctgctttttt atttggaagg
    17221 ccatcaagtt gttcacgata ctcatctata atgttttgaa gctttttcag aaggtctgga
    17281 aaaccagaac ctgtttgatc aagttccttt aaacgagtat ccatatactg gggcaaagtt
    17341 actttcgctt tttctacagc ttcctcaatt tctttattct gaagattatc caaagtgccc
    17401 tctaattcaa ccaatttatc aagctctgtc gcagtatcaa tttggtttct atatttctct
    17461 ttttctcctt cagttaaaga taaccttcct aacagatcat gcaaggtctt tctatagcta
    17521 tctaaggttg aggatactcc ttgaggtctc gctccatcag cagcctggac tacttgtccc
    17581 ccccccccgg cgcagaagaa ggtcgcaacc actacactgg ctacccctac actaaatttc
    17641 cgaatagaat atcggttccg tcgttcatgt cttgctctca tgagtaagtt cctccaatat
    17701 atttttattt ttttactagt ctatcctaca aatgttaaat aaatatgaat cagactttgc
    17761 tttagatagt ttcttcttgt ttaattccaa ctcgaccttt aaaatggctc tcggagaatg
    17821 ctttactaga ttcataatta tgattcattc ctttggtttc actcaaggag tctgggacaa
    17881 aagtctctta tctccaaaaa aagcaacgga tttgccgttg cttttttgca tggttgcgat
    17941 agtcttggta aaatagaatt gcacaataaa ccatttagaa aggctatccc atgcatattc
    18001 accataacac aaatcaaaca actttaccac tagaaatcag ttccttcttg ccacaagacc
    18061 atctcgtctt cactattgaa aaagtggtga ataccttgga ggattgtcat ttccacgcct
    18121 tctatcatgc ctttggtcgc ccgtcttatc accctaaaat gcttgtatct gctcttctat
    18181 ttgcctattc acaagggatt ttctctggac gaaaaattga aaaaatgatg attgaaaatc
    18241 tggctatgca atacctaaca gggcagttgg ttgtcagcta tcgcaccatc aatcgttttc
    18301 gtgtagctgg agggatggaa gaactcattc gcaatctttt catcgacctc aatcttcgtt
    18361 taaaaatgga agagttcgtg accttagatt gcttgtttat tgacgggact aagattgaag
    18421 ctaatgccaa taagtatagt ttcgtgtgga agaaagcaac ggacaagttt gccgccaaac
    18481 ttcaagaaca gatacaagtc tattttcaag aagaaatcac tccccttatc catcaggcca
    18541 ttaagttgga ggaagaagag cctatctcct cggagcagtt gcttgcattc gctcaagtcc
    18601 ttgaaaaaga attggaaaaa ctgaaccaga acattgagga gacagctatt aaagggaagg
    18661 atgaacgtaa aaaccaacgt cggaaactca agaaagtctt gcgtatagtc aaggaggatt
    18721 tttcagtacg tgctgaaaaa tatgagaact atcaggagac atttcaaggg cgtaacagtt
    18781 tttccaaaac cgatccagat gccactttta tgcggatgaa agaagaccat atgaaaaatg
    18841 gtcaactcaa ggctgcttat aatcttcaaa tcgctacgga aaaccaattt gttcttcact
    18901 atgatgtctt ttcaaatccg acagatacca agactcttct gccattcctt gaaacctatc
    18961 cgcatgactt gaagacagtt gtcgcagatg ctggatatgt aagtgaagat aacctccttc
    19021 gtttagatga aaaggaggta aaccatctga ttaaatatgc catgtttgat aaggaacaaa
    19081 agagaggcta taaatagtca gctaaaaact tagggaattg gcactataat gacaaggagg
    19141 atagctatac acatcctgat ggctggtatt atcgttttca ccataccaaa catcagaaaa
    19201 cacagacaga ctttcaacaa gaaatcaagg tttactacgc cgacgaacct gaatcagccc
    19261 ctcaaaaggg actgtatatg aacgaaggct atcaaaactt gaaagctaaa gaatgtcagg
    19321 cgcttttatc tccccaaggt agacagattt tcgctcaacg caagattgat gtggaacctg
    19381 tctttgggca gataaaggct tgtttgggtt acaagagatg tcatctgaga gggaagcgtc
    19441 aagtgagaat tgacatggga ttggtactta tggtcaataa cctcctaaaa tacaacaaga
    19501 gaacgactca aaattaaaaa gctagagttc cacaattgga aatctctagc ttttttgtga
    19561 ttgagaacta ttttgtccca ggctcacttt atctatcatt aatattcttc gaaaatctct
    19621 tcaaaccacg tcagcttcac cttgccgtag gtatggttac tgacttcgtc agtttcatct
    19681 acaacctcaa aaccatgttt tgagctgact tcgtcagttt catctacaac ctcaaaacag
    19741 tgttttgagc aacctgcggc tagtttccta gttttctctt tgattttcat tgagtataaa
    19801 atcccagttt ttcaaagatt tctgagaagt tttggctgat tgtctcaagt gacacttgca
    19861 cttcttctcg agtttgattg ttcttgaccg tcacttgtcc gctttcgact tcactctctc
    19921 ctagggtgat gagggtctta gccgcaaaga catcggctga cttgaattga gctttgagtt
    19981 tacggttgag gtaatcacgc tctgctttga aaccttgttg gcgaagggct tgtaccaact
    20041 ccaaagcctt gacatttgcc acttcaccca agactgctat atagacatct agggcgtttt
    20101 cgatagggag agccacacct tgcttttcaa gaatgagaag caggcgctct acaccaagcc
    20161 caaaaccaaa tccagcagtt tcagggcctc caaagtaagc aaccaaacca tcgtagcgac
    20221 cacccgcaca gactgtcaag tcattgccct caatctctgt gataaactcg aaaatggtgt
    20281 ggttgtagta gtccagacca cgcaccatat tggtatcgat gatgtaatct actccaagat
    20341 tttccaacat ctgacgcaca gcatcaaaat gagcttggct ttcttcatca agaaagtcca
    20401 agatagacgg cgcattctct actgtcacct tatcttcttt ttccttagaa tccaagacac
    20461 gaagaggatt ttcctccaag cgacgttggc tatccttaga caaggtctcc ttgagcggtg
    20521 tcaaatagtc aatcaaggct tggcggtagg ctgcacggct ctcaggattt ccaagagtgt
    20581 tgaggtgcaa tttgacacct tggatgccga tttctttcaa gaaatgggct gccatagcga
    20641 ttgtttccac atcggtagct ggattgctag agccaaaaca ctcaacacca atctggtgga
    20701 attggcgcaa gcgccctgcc tgtggacgct cataacggaa cataggtccc atatagtaga
    20761 acttgcttgg cttttgcact tctggggcga aaagtttatt ttccacatag gaacggacaa
    20821 cgggtgcagt tccttctgga cggagggtga tatggcggtc acccttgtca tagaaatcgt
    20881 acatttcctt ggttacaata tctgttgtat ctccgacaga gcgactgata acctcgtaat
    20941 gctcaaaaat aggcgtgcgc acttctgcgt agttgtagcg tttgaaaatt tcacgggcaa
    21001 agccttcaac gtactgccac ttagcagact cagcaggtaa aatatcctgc gttccttttg
    21061 gtttttgtaa tttcataggg aatcctcttt aaacttaata gtcttatttt accataaata
    21121 aagggattaa aacagtgaga aaaaaattag gatttagata tcatttttga gattaagaat
    21181 tgtcaaaaaa atagctagca aggaaatacc aacaaatagc atccaagtca actgtatatt
    21241 ccatacggct actagtgaaa aacaaactgt tcccacaggt atggataagg taaacaatag
    21301 acctaaaaaa ttactggtac gagctagaac ctctggagct agatttttca tgagcatggc
    21361 actaatcttt ggttgaactt taccagacac atacagagta aagaagagaa atagcaaacc
    21421 aagcacgact tgattgaata aattagccaa accaactaga ctaagtccta cggtcgccca
    21481 catcatcaat ctaggcaagg actgcttccc aaaataatca ttgcccgtaa ggctactgat
    21541 gatgactgct actaaagcac cgacttgact gataaataac gcctctgtgt aagaaaaatt
    21601 caagagagaa tggctcaaaa agaagatatt gtagatgcct cctaaggaac cacccaaggc
    21661 attgataagc aagacagcaa agagcataaa accaaagttt ttctgtccac ttttaagaaa
    21721 aacgagacgc aaatttcgat aaatcgttag aaattggtat ttgatagaaa gcttctcatt
    21781 ttttaagctt tcaccatcag cagatgacat tgacaggctc aatttgcttt ttcctaaaaa
    21841 gaggatagtg gctgatacta ggaaaaagca ggcattgatt cccgcaacga gagaaaaatt
    21901 gttgaccgat agagctaaga gccagactcc gaaagcttga ccaccaatag ctgaaatata
    21961 ggtgataaac tgtgaaaaag aataagcctc catcaaatca tcttcagcta cttttcctta
    22021 ataagaggca tacgcaggcc acctgcaaaa tcactgatga tatcactaag gacattgatc
    22081 aaacacaggc tagaaaaggc aaagagacta gcttgctgaa caactaaggg ctgctagaaa
    22141 aaatagaacc gcctgaaaca aaccgctata gaccatccat ttgaccttgt ccctcgtgta
    22201 atctgcccga atccctgcaa aaactgtaaa gagggtcgga agaatcatga caatattcgc
    22261 catagcaaca gcaaaagatg cttgtgacaa ggtcgatgca tagacgataa agaccaggtt
    22321 gaaaatcgaa gcaccaaaag cattgaagaa gcgtgacaaa gtcaagaggc gataagcttg
    22381 atttctaaac aatagtttca tagataatct cccttcttgt tcgaagttct acatcaagaa
    22441 tagctttagt atagcactta tgaatttgcg aaacgattct tttgaatata tgcaacaaac
    22501 aaaatgagca aaaaaacgat agaacttttg taatgacctg agagagggca acagttctat
    22561 cgcttcaaat tttttcttgg tttgcagata ttcaacattc ggtcgtattt ctatagtatt
    22621 cggcagattt attacagcca agcatctcaa aaatacggac agcatcctcc atctttttct
    22681 ggccttcctt gactctacct tgcttgctat caaggagatc ttctgcccac agataaacaa
    22741 ttcggaaata ggtctcattt tccttgtaga aatgctcttc gataacacgt ttaaaataat
    22801 aggcattgat aaattcttca cactcaatac tagctaaaaa gccattcaat agtatagtat
    22861 gaaaaaggtt tcgattgcca gacatttcca ttagaaaatc agatttacgt accatttctc
    22921 gtacatatct agtaaaaaga gaaacagata aaaatggaga actgactgaa aataaattga
    22981 gttcatagat tccccagatc tcggtagaaa acaaataatc atgaaggact tttccttcct
    23041 ctgctgttaa ctctaccctt tcatctatgc tcttcatata agacttgatc ataatggcat
    23101 ttagaatatg tttctgtttg ttgtgagaat gggcatgctt ttgatactcc ctgcgatata
    23161 agtcctcaag aggtgctata ttctttggtt ccaagacatc tgtaatttct tttctcaact
    23221 cagaatctgt atcatactgg aaacctctcg ccagaaagag gatctcctcc acactggcag
    23281 atatattttc cagagcaaat agaaactttt ccaccgaaag ctcactctga cctgtttcaa
    23341 aacgggacaa catagacggc aaaaattgtc ctccggttgc ttgtctcagt gagatatttc
    23401 ttgactctcg taattgtcta aagacttttc caatctgctc catagacttc cccttgattc
    23461 cgtattttct tcattttatc atatttttca gaaaattcat caaaaacttg ccaaattgtc
    23521 agaattatga gaaaataggg gatatttatc acgtggaggg actgctatga gagacgatat
    23581 caaaatcaat gaccgtgctt tggccttgca agaccaaatt atcgaaaaac tagagaaagt
    23641 ttttgataca gatgtggaat tggatgttta caatctaggt ctgatttatg aaatcaatct
    23701 ggatgaaacg gggctctgca agattgtcat gaccttcacc gatactgcct gtgattgcgc
    23761 cgaaagcctg cctattgaaa tcgtggcagg tctgaaacaa atcgagggta tcgaagatat
    23821 caaggttgaa gttacctggt cgcctgcttg gaaaatcaca cgaatcagtc gctatggccg
    23881 tattgccctt ggactaccac ctcgttaagc aggccaatca cttttaaaga tgaaaaaagc
    23941 aagccgaaaa tggcttgctt tttgatactc aatgaaaatc aaagggcaaa ctagaaaact
    24001 agccgcaggt tgctcaaaac actgttttga agttatggat agaactgacg aagtcagctc
    24061 aaaacactgt tttgaggttg tggatagaac tgacgaagtc agtaaccata cctacggcaa
    24121 ggcgacgttg acgtgatttg aagagatttt cgagtatgag tttatttttt acctgacttg
    24181 tccatattcc agaagtctgt cacggctccg cgtgaagcag atgatacgat gtgggcatat
    24241 ttaccgagga caccacggct gtaaagtggt ggcaaggttg tttctgcctt gcgtttttca
    24301 agttcttctt cggatacggc catggaaatt tctttggtat cttggtcaac cgtaacgata
    24361 tcgccggtac ggagataggc aattggtcca ccatcctgag cttcaggagc gatatgtcca
    24421 acaaccagac cataagtacc accagagaaa cgtccgtccg tcaagagggc caccttatct
    24481 ccctgacctt taccaacaat cattgaagaa agtgacagca tctcaggcat accaggacca
    24541 cctttaggtc caacaaaacg aacaacgact acatcgccat caacgatttc atctgtcagg
    24601 acggcctgaa tcgcatcttc ttctgagtca aagaccttag ctggcccaac gtgacgacgc
    24661 actttaacac ctgatacctt ggcaactgca ccgtcaggag caaggttccc gttcaagatg
    24721 ataagcggac catccgcacg ttttggattt tcaagtggca tgataacttt ttggcctgga
    24781 gtcaagtctg caaagtcagc caagttttca gctacagtct taccagtaca tgtgatgcga
    24841 tctccgtgaa ggaaaccatt tgccaacaag tacttcataa ccgcagggac accaccgact
    24901 tcgtagaggt cttggaagac atactgacca gatggtttca agtcggccaa gtgaggcaca
    24961 cgttcttgaa tcgtattgaa gtcctcaagt gacaagtcaa catttgcggc atgggcaatg
    25021 gcgagcaagt gaagagtggc gtttgtagaa ccaccgagag ccatcgttac agtgatagca
    25081 tcttcaaagg cttcacgagt caagatatct gatggtttga gaccaagttc caacatctta
    25141 acaacagcac gtcctgctgc ttcgatatct tctttcttat cagctgattc agctgggtga
    25201 gaggatgacc ctggcaaact catccctaga acttcgatag cagttgccat ggtattagca
    25261 gtatacatac caccacaacc accagggcca gggcaggcat tacattcaag acgtttcacg
    25321 tcctcagctg tcatgtcacc gtggttccat tttccgatac cttcaaagac agaaaccaag
    25381 tcgatatctt taccatcaag atttcccggt gcaatagttc caccataggc gaaaatggct
    25441 gggatatcca tgttggcaat agcaatcata gatccaggca tgttcttgtc acagccaccg
    25501 atagcgacaa aggcatcgac attgtgacca ctcatagccg cctcgatgga gtccgcgatg
    25561 atgtcacgag atgttagaga gaaacgcata ccaggcgttc ccatagcgat cccgtctgct
    25621 acggtaatag ttccaaactg tacaggccaa gcgcctgcag atttgacacc ttctttagcc
    25681 agtttcccga aatcatgcaa gtgaatgtta catggtgtat tttccgccca agtcgaaatc
    25741 actcccacaa tcggtgtttc aaagtcctta tctgtcatac cagtcgcacg aagcatagca
    25801 cggttaggtg atttaaccat gctgtcatag atgctactgc ggtgacgttt atctaattca
    25861 gtcatcttat ccctcccatt tcagttttta ctattatagc acaattttcg catgaagaac
    25921 agaatgaaat tcttgaattt tcagaaaatt ctatacacat gtgaaatatt taaaattaaa
    25981 aacaacaaag cggattagtg cactttctga tgaccagaat atgcttttta atccgctttc
    26041 tttaaataac gtactgtaat ttttacagaa attctttcag ataagtgtat ttaacatcta
    26101 tcttgcatta taaatttcta gaaccttctc ttttatattc gattcactca aaccatactc
    26161 attaagaaga taatccattt tccctacttg accgaatctt tcttgaacac ccatccgatg
    26221 aatttttgtt attccatcat cagagaataa ttcacataaa gcactgccaa ttccacctat
    26281 ctgattgtgg ttttctacag taaatatagt ttttccactt aacattgttt ttatctgttc
    26341 tggtatcggt ttgattctaa ataaatctat cacacctact gaataaccta atttagacag
    26401 ttcatctgca actcgaatac ttggagcaac cattatgcca gaagcaacga ttacaagatc
    26461 ttccccgtgt cttaactcaa tgtagccttt agaaaaatcc tctccatctt gatagacagc
    26521 ctctggagct tttctaattg ttcgaatata ttttagtcct tttaagtcta atgtctggtt
    26581 caatatttca cgaaattgga tatcatcagt agcttcaaaa atgattgatc taggaattaa
    26641 acgtaacaag ccgatttctt caaatggcat atgtgttcca ccattcatct ctgcagttac
    26701 tcctgcatct gatccaatca cagtggcatc caattgtgcg tatccaagag aaataaataa
    26761 ttgatcaaat actcttcgtg aagcaaaagg accaaatgta tgaagataag gtctaaaccc
    26821 ctgaatagac aagcctgctg caaggccgac catttctgct tccataatcc caacattcac
    26881 ataacggtct ccgaagttct tttcaagatt attagtagcc atcgaacttg acaaatcggc
    26941 ttctaagact actatatcag aataactttg attagcctct aaaaggaaat ctctatatac
    27001 atgccgtaat tctttcgtac ttctcaccat tctgtttcct ccaattcctg acttaatctt
    27061 tctacaactg aagttaacat ttgtttctcc tctacagtag ggcgaagatg atgattggat
    27121 ttcatttctt ccagctcttg aaccccttga cctttaatag tatctaatac aatgcactta
    27181 ggtgatgaat tatttgactg ttttaattgg acaatccctt cataaatttc tctaatatct
    27241 gaacccttga ccctaatgga ttcaaatcca aatgctgaaa atttttctac gaaatcacct
    27301 ggattacaaa tatcctttgt aaaaccatct aattgttttt tgttatcatc aacaaataca
    27361 attaagttgg ataactgttg atgagaagca aactgtatag cctcccaaca ttgtccctca
    27421 tttaactcac catctccaac aatagcataa gtataaaagg gactctttct tattctctga
    27481 ccatatgcaa gtccagttgc aacactaatt ccttgtccta aagagcccgt tgtcatatct
    27541 atgcctggcg ttagatttct atcaggatga gacggtaatt tggttccatt tgtatttaaa
    27601 gaatataaga attctttgtc aaagaaacca ttcaaataga gtgtactgta tagagctggt
    27661 cctccatgac cttttgataa tatgaaataa tctctatctc gtgctacaaa tatttctgga
    27721 gtcattggca ttatttcacc ataaagcact gctaaaactt ctacaataga cagacttcct
    27781 ccgtaatgtc cgaatccaag atgattcaat gttctaagag tatttaatcg gatgttagtc
    27841 gcaaattttc ttaacccatc ttctctattt ttacttaaaa tcatccctta ttcctccttt
    27901 gcagatggct ttttaataaa ggatactcca aacataactg ctaaaataag aacaagacca
    27961 atcacaatgc ctgcttgtga gccaaattga tttaacattc ctaaaataat tcctgataga
    28021 ccaaaatctg catctgagaa agttgagcct tggaaaccaa gtcctcccaa aactggcatt
    28081 aaaaagactg gaagaaaact gattaaaata ccttgtaaaa atgctccaat agtggctcca
    28141 cgaacaccac cagatgcatt cccaatgaca cctgcagtcg ctccacagaa gaaatgaggt
    28201 acaacgcctg gtaagataac aaccgttcct gaagcaatca taattgccat acttactaaa
    28261 ccaccaacaa aactagagat aaatccaatt agaactgcat taggtgcata agtataaaca
    28321 atcggacaat ccaaagcagg tttggaatta ggtacaagac gctctgaaat acctttaaag
    28381 gctggaacaa tttcacccaa aataaggcga acacctgcta aaataacaaa tacccctgct
    28441 gcaaattgac ctgctaattg taaagcataa actagaccac ttgtaccact actgatttct
    28501 ttttctatat attctgaccc tgcaaagata gctacaataa tgtaaataac tgccatggat
    28561 aaagtaatac taacagtact atcacgtaaa aaagctaaac tctttggaaa tttaatgtcc
    28621 tctgttgatt ttgatttgtc accgataagg ctaccagtaa aaccactcaa ccaatatccc
    28681 aaagaactga aatgacctaa agccaccttg tcatttccag ttaattgaac catatatttt
    28741 tgcacaaatg ctggggaaat actcataata ataccgagtg ctaatcctcc tagtaagatg
    28801 agaggcaagc tagtaaagcc agcaactgat aaaataaccg caatcataca tgccatatat
    28861 agagtgtggt gccctgttaa aaagatatat ttaaatcgag taaaacgagc gattaagata
    28921 ttgaacacca tgcctgcaaa cataatcatt gcagtagctg agccatatgt tgttaaagct
    28981 acagctacaa ttgcttcatt attcggaaca actccagata aatgaaaagc atgttcaaac
    29041 atggtaccaa atggattcaa agaattttgt acaattcctg caccaccaga tacaactaag
    29101 aaaccaacaa aggtcttaat tccaccttta ataatatcag gtaatttctt cttctgaaga
    29161 actaatccta agatggcaat taaagctact aaaatagctg gtgtactaac aatatccaat
    29221 atgaacttca tcatgacgct agcctcctat ataagtcctt tttcttcaca aattttagta
    29281 attaattctc gtagttcgtc catatcaata atactattta agatacgaac atctccaaga
    29341 tgactagctg aatcagctag atcacgacca acaatccaaa tatcagctgc atttggatct
    29401 gctccaccta aatcataatg ttcaacttct acatccgaaa cattcaaatc actcaataca
    29461 gattcaatat tcatctgtac cataaaactt gaacctaatc ctgaaccaca agctgtacca
    29521 attttcaaca ttatctaatc ctcctgttta attatcattt taatgtcatc atagtttttt
    29581 gatgatatta aagtttgaac atgattttta tctcttaaaa ttgttgttaa atgtgacaaa
    29641 gcctttaaat gactctcatt atcaatggct gcaatacaaa tcaacaatct tacctcttgt
    29701 tctggattat ccaataaata aatcggttct tccaaaacta acattgacat tcctatttca
    29761 ttcacacctt catctggccg agcgtgagga attgctactc ccttccctaa attaataaaa
    29821 ggtccaaact cttctacttt ttgaatcatt gcctcagggt agttctcagt tatcttatct
    29881 tgatccaaaa gcggtttagc tgctaaacga atcgcctcct tccatcctaa tttttgcgaa
    29941 ctaacctgat aggtttcttt ggtaataagt tgttctagca ctggtacaat ttcctttcta
    30001 tcattttttt gataaagata attctttaac gccaatctta attccaattc ttgtgtaata
    30061 attccatatc ttttgacaat attcaggatt tgttcaatct caaaatctcc atactctaaa
    30121 ttcggaaaat cttttaacac tagttctact agttgtattg cttgctcttc agtcatcata
    30181 accgaaacta gataatttgg cttttctgtc tccaccttta tggtagaaaa aaccatatca
    30241 tagtcactac tagctttcac ctgtaaatca tcaatctttg gggttcctat aaactcaatt
    30301 tgaggaaata atgctaatag attctctttt aacatcaatg aagaactaac accattagga
    30361 caaatgattg ctgctttata ccatttttga ggcaaagtat ctgctttctt taaataacct
    30421 ccgaaatgga taacaaaata tgctgtttca ctatcaggta tgggattgtc aatagcgtcc
    30481 atcaagggca tcaaagaatc tttgactaat tcaaataaat caggataatg ttctttaaca
    30541 tgcaatacat attcatttga actaggtagg ccgaacttta atctatagta agccggtata
    30601 aggtggcggc gaagattttc tctcaatcct tccctttgtt taaaatgtaa caaagaaata
    30661 tcttccattc tacttataat agcctctgtt aattgattaa agtaaaccgg agcaacatct
    30721 acttcacctt caaagcaact tgataataaa actgtgatat agcgataatc atcctcagaa
    30781 aacaccgtat ctataattcc caaatcaacc actgtatcca ataaaatagt ggttatatct
    30841 tgaataacag gagatactaa tgtctctgaa agacatactc tttcaacatc cctttgatac
    30901 ctacacagaa tgaatactaa accgaaaagg caaactttta attgattaac aataggtact
    30961 agctgtagct tctcataata atctttaact acctgattta tcaaatcatc agttaatgaa
    31021 tacccccaac tggataaaac ataatccaaa ccccaaatcc ctatggagga ttccagcaac
    31081 tcactaacca tttgaaaagc taaacggtgc ttattccact ctgaaccgtg taaagtataa
    31141 ccttttgctc tactgtaccc tagctccaaa tcattatcta acataatctt tcttaatgat
    31201 tgaatatcag ataaggttgt attcttactt actttcaaaa agtcttggta atgattattc
    31261 gatataaaat ctaatcggca aaaagtgtaa agatagatta aagctaagcg agtcgacttt
    31321 ggtaaaacca attcatccga cttaataata tctgtcaaag actgcttcgt acgatttgat
    31381 aaactatagc gaccttgctt tttatccagc actatcccct tattagctag ataaggcact
    31441 aaataatcta ttccttcttt gacttccttt ataggtaagc tcaccttaac agataattca
    31501 tataatgata gctcacaatg atccatcaaa gtcatcaaaa taactagtgc tctataatca
    31561 aacatttctc ttcctttcta actacaatta tagtgtaaaa gttataagaa taaaagagta
    31621 tttgagcaca atataatctc accttttaaa agaaattatt gggctgaaac tttttagtga
    31681 ttatttttca actttctatt gactttataa attaaagtga tatcatttag atatcaaata
    31741 atgggaggtc attcttatga ctgaaaaact aatcaattca aaaccaaatg gtgtattcgc
    31801 attgattctc attgagttga cgctcgtact tggtgtcttt atatttataa tgggggttgg
    31861 ttctcaaaat atttttggta ttattatcgg acctttacta atcgtaattg caggtctagc
    31921 tcatgctggt ttaaaagttg tcaaacctca ggaagctctg gttctgacac tctttggtaa
    31981 ttatacaggt accatcaaag aacctggttt ttactttgtc aatcccttca gcgtagcagt
    32041 caaccctgca aaccacactc gacttggaca aagtggtgat gttagcacaa aatttccttt
    32101 tttaggagct aaatcatcaa atgacaatga tgtaaatctt gaaattagca aaaaacacat
    32161 ttccctcaaa gtcatgacct tgagcaattc tcgtcaaaaa atcaatgatt gcttaggaaa
    32221 ccctgtagaa atcggtatcg cggtaacttg gagagttgtc gataccgcta aggcagtctt
    32281 caacgttgat aactacaaag aatatctttc attgcaatgt gatagcgccc tccgtaatat
    32341 tgtccgtatc tacccttacg atgtatctcc taatgtggat actacgggtg acgggcaagc
    32401 agatgaaggt agtctccgtg gctctagcga aattgttgct aaccgtattc gtgaggaaat
    32461 tcaaagtcgc gtagaagatg ctggcttgga aatccttgaa gcacgtatca cttacctagc
    32521 ttatgctcca gaaattgctg ccgttatgct tcaacgccaa caatcatctg ccattattga
    32581 tgcacggaag atgattgtag atggtgctgt aggaatggtt gaaatggcac tagaacggct
    32641 caatgaaggg gaattggtag aacttgacga agaacgaaaa gctgccatgg tttcaaatct
    32701 ccttgtcgtt ctttgtggca atcatgatgc acaaccaatt gtcaacacag gaagtcttta
    32761 ctaaagatgg ctgaagctaa aaaaaagcag atcccccttc gactctcaac aaagttatac
    32821 gctgcactcg catcatgggc agaagacgac tttcgttcag tcaatgggca aatcgaatat
    32881 ctcctcacag agtgtgtcaa gcaacggaag aaagacggaa aatacgcatc agaaaccatt
    32941 gacgaaccat ttgagattga tatttaacat cttctcctgt ctgacaagtc ggacaggaga
    33001 attttttcat ctttttttgt caagtaactt tactttacaa acaaaatgtg ttatcctagt
    33061 atggttgatg aaaatcagta gattgaatcg aatataaata cctaaaggag aaatcaaaat
    33121 ggcagtacct gcacgtcgca cttcaaaagc gaagaaaaac aaacgtcgta cacactacaa
    33181 agtaacagct ccatctgtaa actttgacga aactactgga gattactcac gttctcaccg
    33241 tgtatcactt aaaggatact acaaaggacg taaaatcgct aaagctgcat cagctgaata
    33301 atagaaggga gataccatgc gcgtaaatat tacacttgaa cacaaagaat ctggtgaacg
    33361 cttgtacctt acttctaaaa acaaacgtaa cactccagac cgccttcaat tgaagaaata
    33421 ctcaccaaaa cttcgcaaac acgttgtgtt tacagaagtt aaataattga gtaaaccaaa
    33481 agcccttgaa accaacattt caaaggcttt tttctttttg ttttaggaaa ataactgaat
    33541 tttaactgat taaatattcc gtaatgatat atgaataata aaaaacggat aacttctagt
    33601 cttgattaaa gtttaaattt ggtatcagaa cagctccgta tgccccttca gaaaacatac
    33661 caacatataa tctaaacttc tctatcattg gtgtaataag tgcttgttga aattcatcat
    33721 cctgcgaaat atcttctggt aaatctgaag ctaagaccga atatctggca tcaatgtcag
    33781 caataactcg atactgacta tctgggtatg aaatttccat ctttatcaga ataagttgat
    33841 tctcttgtcc ttcctctctg tcaggaaaat ttaaattaat cgaagctctt ccatcttctt
    33901 caacttctcc agtgttatga gtattaaatt caaatttact aaatgttatt cttcttatct
    33961 caatattttt tgtcttcatt ttaacctcca atagaccatg aaactacttt attatcttgg
    34021 agtctattat tagtaatatt ctgctcttta taaggttgta ttttttcaga ttgatttttt
    34081 ataatgattg tttggtgaat atgttgctct ggtgcagaaa ccttttcagc aatggtttga
    34141 ctaagttttt tagtcatatc tttaaattgc tctgttagct gttcaacagc attttgagtt
    34201 tgtttgaata acccgtcatt aatcaattgt actatagttt gatttaagct gactccttca
    34261 atttcagagc gttctttaac atctctatgt aaggttttag gtaatcttag cgttattttt
    34321 ccactatact cttccagaga gttctcaaaa ggttctttaa tttttctccc ccactcaagg
    34381 ttggcctcaa tccagtctgc tttagctaat tcaatctcat cataaacttc tcctaaagat
    34441 tcaccataaa cgattaatcc tggtaactct ttaatagagg cagtaaacaa taattcaccc
    34501 tcgtcataaa ttttttcaat taaaatcgga tagttcaatc caagataata ttctttatct
    34561 ttcatcattt aaaccctcct ccaattctat cactttctca atcattttta taactttctt
    34621 catataaact ggctttatcg gcttatgtcg cggtattgga tagctttcct ccaataaagc
    34681 atgagaggtt attgaatgat tggttccttg cttccacata aaaccatatt ttttacacag
    34741 agattctaag cgttcagggg taacagattt ggggttattt tttatttttt gcaactcttt
    34801 ttccttgccc atgatgtacc tcatttacag cgacactata tatggtgtca caataattat
    34861 atcccaaaaa ttcgctaatt tcaaggattt cttttaattt taataaaata tctgaatttc
    34921 ataagataat taaaaaatta tttcagccaa atctatcata gcttgttcaa tcttatcttt
    34981 tcccttttgc ataacatgaa ggtagatttt ttctatctct ctactatctt ctgaatgtcc
    35041 tacaaattct cgaataacgt ttaggcttac attcttctct gccataactg aaacaaagct
    35101 atgtcgaaac atgtgaggaa ctatatgaat aggtctacca aaatctacta agtcatcaat
    35161 atctttataa ggttttccgc ttttcttatt atactttgcc aactggctag cttcacctct
    35221 taagaacatt gaaatttctg tatggtacat tgggctacct tgttgatttt tgcctttaac
    35281 gtagattcta gtaaatatat agcccatatc tttataagtt ggataaataa taccattaag
    35341 gaaagtgcaa gggcgcattt gattgaaagc cagggagtga aggcagtaaa gtcaatgcct
    35401 tttgccgtaa atccagcaaa ctcaagccgc taccagctga agataaagcc tataaccatg
    35461 cgctatccaa ggagagaagc aaggttgaga acatctttgc caaagtaaaa acgtttaaaa
    35521 tatcttcaac aacctatcga aatcatcgta aacgcttcgg attacgaatg aatttgattg
    35581 ctggtattat caatcatgaa ctaggattct agttttgcag gaagtctatt gaaatactaa
    35641 tcctacacag actgattcca attgtttttt agatttcaat tttaccattc ttcatagtca
    35701 aaaccatcat aatctggata ttgttctagc gacgttccaa ccgaacgtac gagataagct
    35761 tcttcgtcct ctgtctagat ttctctcagc acttggcatt caaaacgcca ttcatcaccg
    35821 aagtcgaaaa taaacttaaa tttttgtttg acacttaggc tatccagata gacattttct
    35881 gtgtaacgtt cttcaacatc gtcactaaca aagctaagaa agtaagaatc tgcatgactc
    35941 cactcaacat tatccatgaa aaatgcgtga gcatgatcaa ttgcaaaatc aaaactccat
    36001 aaaatcacat ctgctagttc cgctaacgtt tttgtttttg gaatctatat atgtcggtag
    36061 caaccttttt taaatttgac ggaaatgatc caagcttcta cagatgctgg cttttcagct
    36121 tctatttctt gttttactaa ttttaagtgt agcataatac ctccaattgc tcatcgatag
    36181 aattgctctt aggaaacatt cactttctgc tattttatca taagtttgtc taactgtagc
    36241 atttgaaacc agataattct tctgtttcac gcgaaacacc ctgcgccaag caaggtattc
    36301 ttgtttttat actcaatgaa aatcaaagag caaactagga agctagctgc aggctgctca
    36361 aaacactgtt ttgaggttgt agatggaagc tgacgtggtt tgaagagatt ttcgaagagt
    36421 atgaagagta ttaagtcgta taaatcgtcg acgctgtcgc aatggtatgc cactggttag
    36481 ctgtagttgc tgcgaagtcc ttgtctgcgt agaagtcagt aaatggtagg atttcaactt
    36541 ccagctcgtc gatgcggtca agctggccag ccagataaac ctcgatacgg ctttctgctc
    36601 gtttgatgcg ttgcaagagt ccgcccatac ggatgtcaac tgtatccaaa ccaaagacct
    36661 tgttttcttt cagccattgg tgactaaaga gggcatggaa gtcttcaatt tggcttctaa
    36721 gttctggtaa ttcttgtctg gcgatttgtt gtaaactttc tttatcatcc gcttggtagg
    36781 cctgacgaat gcgtcgtccc acatctactt tgctacttaa aatagcattc aactgggcct
    36841 gagtttcaaa gagataagca tagtttccag ctttttcttt aatgtcagca atagttcccg
    36901 ccgcctgagc gaagtgtggc ttgtcctgtt caggtgtcat gtgtcggtca agtatcggac
    36961 agagaacatc ctgataaaag acatagcggt tgggattgat gccactgaga ttacctggta
    37021 ggtctggtaa gaggttggca aggtcaatct gcataaaatc ctcaaccgtt agaccagtat
    37081 tggtcttgaa atgcgcagac aaactatcta ggtcattgcg atagctgagt tctgcccaga
    37141 tttgcaagct tggtaggata gagaactggg cagtttcacc accattgtct ccccaacccg
    37201 ttacgatgac ttctttaatc tgattggcac ggcaggcttt attagcctcg atagctacta
    37261 gacggctaaa atggttgtga ggtgtaaagc caatccactt ccaagctccc cctgcaaatg
    37321 caaggtcatg gctaatcttg tgatgattgc ggaaattacg gttgtatttt tcctcgctat
    37381 cctgataata atcccagtaa accagagtca cacggtcttt gagacggtct aggtagacac
    37441 gagtttcctc tggaatttcc acatcacggt cgtactggcc atccgctgac atgagtttga
    37501 agaacatatc actccacatc tggcagtgga aaccatattt gtcagcaata tccagcacgc
    37561 gctccaagtg ttggcacatg aggagactac gattcacaac accgtccaga atcaggtagc
    37621 gtcccaaacc aaccaagtgg gcttcatcca tcccgatatt gaccttgcga gtcttcagtt
    37681 tagacaacgt ggcaaacatg ctatcaatca ggtcataaac cttttcttcg ccaatgagaa
    37741 gaatgtcctc tacatcacgg agctcttgca cttccttgac accccatttg acgaaggctg
    37801 acaaatgggg caaggtctgg atgcaaggca caaaggtcat gtcaaactgt tgggcatagg
    37861 cttcgatttc ctgcaactcc tcaactgaat aagctccacg gaaatagcca aagtaaggtt
    37921 gcccttcaat ctggtaagtg tcttccatgt aaagctcaaa ggttgagtag cccatgagag
    37981 ccaatatctc aatcatctgc ttggcagaag cgacatttag caccgcattt cgagaacagt
    38041 caaccatgta agccaaatct tcgtaagctg cttgttcctc aatctctact ttatcagctt
    38101 ctgctagaac tgttaccaac aaggacaagg cacgataaag ttggtgaggt ttgcggtagg
    38161 tcaattgata gtgaccatcc tcacccttga tagagataga ggcttggtca gactgagcga
    38221 ctgacacctc aacatctggc agagaaatgt gaccttttaa aacctcaata gcttgcgttt
    38281 gtttgggact aagtcctgta aatcttacca ttgcttttcc tcctgtagcc agttgacaag
    38341 ggcaccgtag agattggcat ctgcatgata ggtgcaggcc tggataactg gtgcgaccgt
    38401 gtattcttcg taggtatcga caaagttatc aacagccttc ttaatacctt ggataaaatc
    38461 tggattttga ctgatagagc cacccagact gatgacatct ggatcgatca gatactggat
    38521 attgagcaag ccttgcgcca gattgcggtt catgcgctca atggcttctt gacaaagagc
    38581 attaccagct gcggcctctt ggtaaatctt gcgaccgtcc caatcagtat gaccagattt
    38641 ttcaatcaca tatcgtacca tattcccagt tgacgctagt tgcgaccagt tattaagttt
    38701 ttcagcaggg gcaagggttg tcatgtagcc aaattctcca cccagaccgt ggcgacctcg
    38761 atgaagtcta ccattgatag tcatggctcc accaattcct gtcccaatca cgacacaggc
    38821 tgcattttca agctctggat gagctagtag ttcactgagt ccaacgcagt tggcatcatt
    38881 ttctaaatgg acaggtagct gataagagct aagcgcctca tgccaagaaa agccatggat
    38941 gtagggcacc gcactgaagc catcaatcac acctgtctct tgattgactg cacctggaac
    39001 actcatagca atcccactgt aatcctgttc tgacaagcgt tgatctagcc acgctagtaa
    39061 atcctccaag ttttcaggcg ttgaaatact tgtcttatcc agtattttcc catcaggagt
    39121 cagactggca aacttaatcc cagtccctcc gatatcaatc gttgcaatgg tcattgtttt
    39181 taccataaaa aggggcgaat cagcagttag ttcttacttt ttcgcccctc ctttcttttg
    39241 atgtttactt tttgaaatta aatttcttct tttttgatga attctgtacg aatctcttgt
    39301 ggagccaata gtcctgaatg aactggatat ggtcgctcaa gtaagtcaag aatggtttgt
    39361 tgatgttctg atatgcgcac attttcttga ctcatattat agtaacggag gacatagcct
    39421 tcttcatttt ccgccacttt aaaggctgtt ggacaaactt gcggtatgct gagaacagaa
    39481 tggctcaaga ggctaccagt cgcaaccacg cttccttcct gtctagcaag ctgaaggctg
    39541 gtaaacggtg tctgcaaggc tttggcacga cgataggctg agaagcgttc ttgggcttgg
    39601 tggcattcaa gtgcaaactc gacttcaaac tcacgcaagc attgtgcttc tggcgttggg
    39661 aagtagcccc agtcacctag ctcacctgat gcacgcaaaa tggtcacggc aatggtgtta
    39721 tccccaagga tttcgtattc attcaatccc ttattggata cagtcactcc tttttcatcg
    39781 tcatacagac tgacaaaagc ttgttgatgt tgaggatttt cccatgaagc tcctggtttg
    39841 tttggtcgtg tcaccacctc atagatgctt tcagaatcat tgcttggacg cgtgttatga
    39901 gtcttgacca agagacggat acggtgatcc ttggcagtgt tagtaaagcg agtcttgaag
    39961 cggatttgtg gattgtcaac aaagacagta aactcagttt ccagaggaat gtttgtcaat
    40021 tcttctgacc gtccagcctc acgcttcata aactcgatga tacctttttg ctcttcttct
    40081 agcttttcat ctgcactgac aggcacggtc aattcatgtt tgagcaaaat cttagcatag
    40141 cgagctgtgt tttccaagac ctcgtagctc ttaagctctg caaagattgg ctctgttcct
    40201 tttggttgga aatagatata ctcgtttccg atgtccccac ggtcttcaaa gcggataaag
    40261 tcttcataag cttcgtgagt tgtcttgtca taaactgtga tattgtcatc tacactcacc
    40321 gttacgaatg gtgtatcaat cactccgttt tggtaaatac cctcacggtg ttcttgtttt
    40381 ccttccagca attggaaggt tgtccaagaa agcggtgcta ggtgaactgg aatggtcacg
    40441 cgcacttgtc gagcaatacg agcttggcgg aacttatctt ttggtaaatc gtactcaaaa
    40501 ttagctccga ggtcttcgat tgtagcctct acaggacgac catccaagtc ctccacacgg
    40561 tagcttggca agataagagc agccatcttt ttgtagcctt ctgttgggtg caattccttg
    40621 aaatcacaag tcgccacatc aatcactgtg ctgacagtat cgaccttatc atgcaagcct
    40681 gtgttaatga cagtaaagag atagtcactt tgagccttat ccgtagcaat tttacccttc
    40741 cactcgttga gcaagttact tttaacaaag tttcctactt ggttgacctt ggcaaaacgc
    40801 gtttccatct cgcggtgaac ttcgtccacg ctacagccac agatactatc atgtggcgca
    40861 ttctgcaaaa gtgttttcca agcataggtc aactggtcct tgtggttgtg tccaccagtg
    40921 ataatagtca aggattctac cacttgctct aggaggttgc tattttcttg gaaggcttgt
    40981 tttaggtaaa tgcgagatga agaagtgttg gcaagtgtgt accagccatc tgtttcctga
    41041 ctggtcaact cgcctgtaac agttgataag tgttcaggaa gcgcaccttc tacagcttga
    41101 acatattcat caaaagaatt atgaacaaag attacatccg ggaagagttc atttgccaca
    41161 cgaatggctt cgctcagatt tttctgtaca ggctggtggt cacagccgtt catcatcaac
    41221 catcggttgg tcgaagcgta ggcacgcaca tctgacaatt tttgtttcca gaaggtcaag
    41281 gcctcatctt tgtcaactgg aatttcattc ccgttactgt accagttggc aaagagaata
    41341 cctaaaacac gactaccatc cacaccctgc cagtacattt ttgaaaactg ggatgtaaac
    41401 tgctcatctt caaggacttg gttgtcaaat ccaatctgct tcacaccacg accaaaggcg
    41461 gtcacgtgaa tgcctgattt ttgaagaatt tgaggcgctt gtcccatatt tccaaaggta
    41521 tctggaaagt agccaatctg agttgattta ccccatttgg cagcttcttg ttgaccaatc
    41581 aaggtattgc ggacattggc ttcacttgaa atcaaataat catcctgcaa gatgtaaaag
    41641 ggaccaattt taagcttgcc ctcgtcaatg tagcgttgga ccttgtcgcg attttcaggg
    41701 cgaatttgta agtagtcgtc aaggacaata gtttgtccat ccaagtggaa actcttgaac
    41761 tcagggtcat tttcaaagag atcaaagaga ttgtcaaaca attccaccaa ctgcatacga
    41821 tggctttcaa aaggcaagta ccactcacgg tcccagtgac tatgtgagat aatatgtaca
    41881 acaacatttt ccatgaagta aaacctcatt ctaaatttaa attttaacgt tttaaatgta
    41941 aacttgtgat tctgataaga atcggttgag ctaatactca acgaaaatca aagtgcaaac
    42001 taggaagcta gccgcaggtt gctcaaagca ctgctttgag gttgtagata aagctgacgt
    42061 tgtttgagag attttcgaag agtattagcg aatatccaag taatccaaga ccaactcaca
    42121 gaacatcatg ttagcccagg agaaccattc acgagagtag agggtcggat catctacgtg
    42181 gaagctttcg tgcatgacac ctgtaccacc atcgcaggca accagctgat ccagcaagaa
    42241 ttttttctct gccttatctc ttgttgtcaa tccttggata gaaagggcga ttggccagat
    42301 atagcgatag aaggtatgag aacttccgag accgctagcg tattctcctt ggtagaagta
    42361 tggattttca gagctcaaaa tggtacgacg agtagcttga tacacttcat catcgaccga
    42421 acagtagccc agatagggcg cagctagtag acttggtaca tttggatcat ccatgatgct
    42481 ggcatttcct aggccatcca cttcaaaggc ataaatcttt tcacccttac tgttggtggt
    42541 gtaagcatag ttttcaattc cttcttggat ctcatcctga agacgcttgg catcagcaat
    42601 aacactctgg ctatcagcta ggtttaatgc tgcgaagatt tcttgcacat aacccaagac
    42661 tactacagca aacatatttg acggaatcaa gtaactatac tggcaacagt catcactcgg
    42721 acgaaaagct gaccaagtca tacctgtcac tgcaaagtca ggtccaaagc catcatttac
    42781 taaggtgccg tatctcggac aaaacgataa ggagagttct tgtggtcttg ttcaactgtc
    42841 catagatgga gaatttcctt agtcgctgcg acaaaagttt catcaaactg actagtctcg
    42901 ccagtctctt tccagaggag ataagccaac tgcaagggat agcaaagtga atccacctca
    42961 tacttgcgct cccagatcca gccgttaagg tctgtgtggt cagtctcgtg gtgcccttta
    43021 cagttctcct caatgttgaa ggagttagca tagggatcct tgagtaccaa ggtcatctga
    43081 cgtttgacca aacctgcaat ggtctgacgc aggagggcat ctcttttagc tacatgaagg
    43141 tagggtctga gttaggctgt cgaatctcga agccacatgg caggaatatc cccagtcaag
    43201 acaaaagttg aaccatcttc taagatttca accgtattgt ccaaggtatc ggtgtagcaa
    43261 cgctcgaaaa catccaccca ctctggatgg tccttagccc gctctgctac ttcatctagc
    43321 cattctctaa caatttcttt cgaataaatc atcgtgcaat ttcctctttc aaacaagttt
    43381 cattttcagt atatagcttt tgagacagaa aatacataca caaatgatag tcatttttct
    43441 acaaaatagt tatctttaaa actccttttc taaaggtcct ctttttctga tataatgtag
    43501 aaaaacagct aaaggaaaga ctatgaaacc actacttgaa atcatcgata cccgctttgg
    43561 aactaccaac aagcatgcct tttctcgggg aaataccctg ccatacacag gcgtgccttt
    43621 tgggatgaat tactttgtgc cccagaccag tgaccaggat ggatcttggt tttttgatcc
    43681 gcatctgcct atctttcagg ggattcgctt aactcaccag cccagtcctt ggattgggga
    43741 ctactcttgg ctccttctga cacctgtcac aggtcagctt gggggagaca gtctctttca
    43801 ccgccaatct tcctatgata gggataaggc ctctttccaa cctcactatt tgaagctctt
    43861 ttctctgcgt tatcagattg aaacccagct cacaccgact tgctacggtg cttctattcg
    43921 tttgaagcaa aagcaaggca aagccctctc cctctatctt cacgcagcag ataaactgac
    43981 agtagatcaa atagataagc ggactcttgc cctgcgacaa gaaggtaaaa ctgaaactaa
    44041 caaaaattcg ctaacgatgt tcactgccct gcaaatgaac acggatattc ttgctatcag
    44101 ccaagaagct ggagactggc gaattgactt agtaagtagt caaaccgaga tgcaactagc
    44161 cacttctttc atctctcctt ctcaagctct aatcaatcta cctcaagaag attttgatag
    44221 ctgtaaagca agtgcccaag cggattggga aaatctcctc catcgttttg atgttataga
    44281 gacaggagag gctaaccgaa ccttctttga ccactgtctc tacagactct tcctcttccc
    44341 acagactttt tatgagatta atgagtcagg gcaagccatc cacatggatc tggctactgg
    44401 tactgtcaag cctggtgtcc tctttagcaa caatggtttc tgggatacct tccgcaccac
    44461 cttccccctc tttgccctta tcataccgga gcactatcaa cgctttttag aaggtttcct
    44521 caatagctac cgcgatactg gtttccttcc aaaatggctg gctccagatg aacggggtat
    44581 gatgccaggt acactgctag atggcattat cgcagatagc gcctgtaagg acatggcccc
    44641 cgacctagaa ggagaactct tccaagccat gctcgaaaca gccagcaaag ccgaccctct
    44701 cggcatcaat ggccgccacg gactagccca ataccaagaa ctaggttacc tctctaccga
    44761 ccaccacgaa agtgttagcc atactctaga ctatgcctat agtgattttt gtatcgccag
    44821 ctgtgccaaa aaactagaga acatagaaat cgctgaaacc tacaaggctg cttcacaaaa
    44881 ttaccgccag ctatttgacg ctgagacagg ttacatgcga gcacgagaca atcaaggaaa
    44941 ctttcaccct gacttctctc cttatagttg ggggcgagac tacgctgaat gctctgccat
    45001 tcaagctact ttaggcgttc tccacgacat ccctggctta atccaactga tgggtggaaa
    45061 agaaaccttt agcaactatc ttttgaaagc ctgtcaagat gctcccctct ttgaaacaac
    45121 aggctatggt tacgaaattc acgaaatgag cgagatggct actgctcctt ttggacaaat
    45181 agccatttcc aaccagccta gtttccacat tccttatctc ttccgctaca gcgattaccc
    45241 tgactacact gcccttctta tcaagactct tcgtcagaaa gcctttcagc caagttggga
    45301 agcctatcct ggtgatgaag ataatggcag tctctcagcc tggtacatct ggtcagctct
    45361 cggtttttat ccgacctgtc caggaaaacc aagctatgac ctcggaatcc ctctctttga
    45421 ccatctacga gtctacctcg ctaaagaaga taaatggctg gatatccata ctaaacaaaa
    45481 ccacaaccat tttaactttg tcaaagaatg ccgactggac aaaacactcg tatcaactat
    45541 tcaacaccaa gacttcttaa aagctgaaca actaactttc accctcagct ggctaccaag
    45601 tcagccgtca acttcctgaa atgcaaaaat ctcctagcag gctttcctgc taggagattt
    45661 tcttatgaga ggtatttgtt taatactcaa tgaaaatcaa agagcaaact aggaagctag
    45721 ccgtagactg tacttgagta cggcaaggcg aagctaacgc ggtttgaaga gattttcgaa
    45781 gagtttaagc tttaaaaatc ggatttatta ttcgataatt atcaaacaat ctaccaatta
    45841 agcttcttcg tcttctttac gacgacggcc tgcaagacca aggtaaggca cttgcggctg
    45901 ctactaccat agctacagta gaatctgctg tacctgtatt tggcaactct ttagcgactg
    45961 tacgcgcgtt tgctgctgct tggttggcat ttgctgcttg agcagtagct ggagctacag
    46021 ctggtgtttg agcagcttgg tccttagctg ggcttgtatg gtcagttgct ggagaaactt
    46081 tagccatgaa gtcttcgata cgtttaacca ttgcatccac ttcatcttgg cttgctttcg
    46141 gatctgcaaa tactttctta gcgtctgcca ataagtcagt cgcttctttt gcagtttctg
    46201 catcaagctt agctgattct tggataatca attcatctaa ttgatggata gcaccttaaa
    46261 cacaagaaag gtacagagat gcaaaaccat tatactacaa aaggcaaaca tttgacaatc
    46321 gatagccgtc gcttaatcga aagatggaaa aaaagaaatg aattatgaag atattttatc
    46381 tcgtttcaac taaaatatga gcttgatcaa tcagctgtcc accaacagtc aggttcagat
    46441 aaaaatccaa gatcttgtcg gcagcaaaat acagagttgg tatgctcaag tcttttttgc
    46501 tttcccctgc ttggaattga aggacttgaa tctcgtcctg ataggcgaca ccatggacac
    46561 ctgtccccgg ttgaatcgaa atcttagcct ctaaagagga gctactttca ctacgttcaa
    46621 tcctaaaatg aagagtctct ccctttgtaa cagctaggct tttttctgca aatgctagtt
    46681 cctgaacaac ttcttttttt gataatccag gagttttata aagggaaatc ttggtcaata
    46741 caggaataga ttgtgcctct gtaatcatca cgcgcacctt ctgtgcctct actagcggac
    46801 ctcgcaagag gcgcttatga ccaactgtga agcccctgcc gaattcttgc cagacgtcat
    46861 ccacctctac ttgcacataa aaagtagcga ttcgttgccc tagcttcaaa tcttctctta
    46921 actcaattac atcaaaagtt ttaggagaac ctaagtcgag ttctaactgg attggcaagc
    46981 ctacatcgct tgccccagag ctagttttaa ggccatctgt cagatgatga caagcaaagt
    47041 ctacggaaag agctgaacca gatatctcag ctcccagagc caaatctttt ttatagagct
    47101 cattacgata ggccgcaaat tcataaaggc gttcaacatc ctttgcatca aagagcccat
    47161 ctttattcgg cggaatatta agcaggagtg gagttcctct cccaaccgag tgaaagtaga
    47221 tttctaccaa ttcctcgaga gacttaggat actgatcctc atggtagaac cagcccggcc
    47281 ggatagagac atctgcctca ccaattgaaa aaatcgtacc cgaaggatct ccgtgctgaa
    47341 gatagtccag ctctgcttct gttcctagtt tgtcaggatt caccttttgc cataggggat
    47401 cacctgcata gcctcgttca ttaccaatcc agcgaatgct agtgccttct gttgaaaaaa
    47461 tcaagcaatc gccctgcagg tcacgaatgg tttcaaacca tgtctcaaat tcataattga
    47521 ccttttgcgc gccctctcct ctggcaccat ccatccaaac ctcagcaaat ttccctccat
    47581 ttccataggc aggatttgat aagatttcct tcagctgagc cagataatag gcattgtagt
    47641 ccgcttctcg gtccacatga tagaggggac tatgggcatc ccacggtgac aggtagaccc
    47701 ccatatccat atcaaactct gtggcagctt gagatacttc aagaagcaag tcgccctttc
    47761 ctcttctcca aggactgacc ttaatcgaat aatctgtgtg agctgtcgga taaaggacaa
    47821 agccatcgtg gtgcttgacc accaaaatca actttttgaa gcccgtttcc ttgagcacac
    47881 gaacccactc acgtgcgtct aactgtgtcg ggttaaagcg ttcaggatcc tcctgacctg
    47941 ttccccattc ctgattataa aaagtattag gtccaaaatg gataaaggct actagttcat
    48001 cctctaaata cgctaactgg acctgactag gcaaggggct atgcggtttt atttctctta
    48061 ccatactact tcctatctac attttcttaa ctttttggca tgtacttgca attctgttaa
    48121 cgctagtgga tttaccaagt tttcaaatcg aagacgaagg gcataggttt ccaccttgtc
    48181 aaattcaaaa cgaagctctt catccttgtc acctgacact tttcggatag ttgataccgg
    48241 tcgccaattc tcctcttgtt tgagagcaga atccttatcc aaatggtttg gagtccgagg
    48301 caaagtaggc tcattcccta tgtaataatc aataaagcta ggctccaccg ccacgtattc
    48361 ttgattctca acataataca aggtcaggtt atccacaaat cgcggtttta aaatccctac
    48421 atttccaaaa agaataccca cgctggcttg ctctgacttg gtaatccaag tattggctgt
    48481 cgatttcttc cgagaaatga ccttgtcatt gaggtaagag gcagggtgat tcgattctga
    48541 gtgagaagca aagaccagag gtaggttgga gcctgtccac tgattggaaa taacttcccc
    48601 atctacacta gcatctgtca catgaatggt gactgttgct ggtaacttac agcccactac
    48661 ttgtcctgtg aacacacatt ctcctacctg agcatagact tggggttcga gttcatccta
    48721 agtcaccttg gccgtatctc gtctgccatc tgataagact agcttaactc ggtctggtaa
    48781 atgtggggct tccttgacaa gcgtccaaac cctctcagac tcaatagcac aaatcccttg
    48841 aacacaaacc tgagcagaag cctctaaatc cagaccctct agctgaccag ataccgtaaa
    48901 ctcatgaaat cttgccaaat cttcttcaag aatctcctcc cagacaacct tgactcgttt
    48961 tgcaccgcct gactcatagt ctaccaagac taaaaatggt aactgtggat aagttccctt
    49021 atctgtatac aagcgaagtg gtcttacaaa aactgcctgc cctagtgagg tattttctgc
    49081 aatctgcaaa tgagtttgat aggtcttccc ttgataaatg gcgtgaatag ccaagtcgcc
    49141 tgcagacaag caatgaagta ctccctgctt gataatggcg tgggcactac cactcgtctc
    49201 ccaaaacact tcactattcc ctaccttgtt cacagggtta tccacagttt gtggataaag
    49261 tccaatagct ggggaagctc cttcttgcaa acctgcctga tcgtccactc gaatctccaa
    49321 caagcgctga tttttcactg gctggcaatc aaaagcctct cccactaaga gatcaaaact
    49381 tgctggctct agccctctgg cactagcttt tactcttact tgacctgcct gctccaagct
    49441 ctgagcctga taacgttcac gactggcctg cctgccgttg tccacaccca caatacgagc
    49501 tggtccttca attttgaaat gaagctgatt ggaagcactc ggcacccaat ttccatcctt
    49561 gtccaataca tcaaaataga gatagaggag gtcttgccca tctggctcta gttgtgttct
    49621 ctcggcatgt aacccaattt tagcaggctt acccgcagtc accaccctat cttctgccac
    49681 aatctgacct tgacggtcat aggccacaac acgcaattct cccggttgat aagccaaacg
    49741 ccaccggttg ataagccaaa cgccattcga gatagagttg gtcagaacct tctccctctt
    49801 gatattttct gccatctgaa gtccatttct cttgaaaagt cttcctgcct tgagattttc
    49861 cattgagaaa tagctctact ggaccagcat ttgaatacac tcgaaccggg atatccccat
    49921 acttatccac agcttgttga taactcttgt ggatttccca attccagtgg ggcaggatat
    49981 gaaccacggg atgctgatct aggtccaacc actgactttg atagagataa taatcatttt
    50041 taggaatcct cgccgtgtcc acaatcccaa aataagaact cttgacaggc gtgtcatttt
    50101 gattgtgcca aggggtcggc tcaccgatat agtccactcc tgtccagata aactgacctg
    50161 cgtagtctag cctatttcta tctgctatca aagaagccgt cgctgtcttc ccccaaggaa
    50221 ctcgatcatt gccatagtct gactgttcaa aattccgcac gcgacgatta tccccaacct
    50281 attccttatc tgggcgaaaa tagctatcac acgttcgggt agccgatgag gtttcagacc
    50341 cgtaaagtcg ccacttggga tgtcttttgc gaatcccgtc aatattatct tccgagtaat
    50401 tcaaccccac cacatccagc aaatcagcaa tcttttcatg acctccagaa ccatctccaa
    50461 agcgaaattg atccattccc atggtcacaa aacggctatc atcaacctcc ttaacccttt
    50521 ctagcaagcg tttgacagtt ttcaatgaat gaacatcgct atttgcttcg ctgacttcat
    50581 ttcccaaaga ccacataaaa atcgctggat tgttcttgcc acgctcaatc atagtgcgca
    50641 aatcgtaatc cgaccagaaa tcgcctgctt tcgcttctgg atgagtcgct tcttcctcaa
    50701 aaaagcgacc atagtcatat tctttcttgc ctccatacca gatatcaaag gcttcttctt
    50761 ggatgagtag acccattttg gcaaccacat ccagcaaaat actactggct ggattatgcg
    50821 tgattcgaat cgcattgacg cccatttcct tcatctggcg caagcgtctc tcagcagctg
    50881 acctgttttc catagttccc aacgctccgt agtcatggcg caaacaaacc ccatgaatct
    50941 tcaaccagcg accatttaga aagaagccct tatctgcttg ccaatccata tagcggtaac
    51001 caaaattctc ctcttcttca tcagctaaaa tgccattttt gtaaaggcga gtcttcaatt
    51061 ggtaaaggtg aggggaatca atatcccaaa gcaaaggttg gaaaattgat atggcttgtt
    51121 ggaaatgatg tttttcccca gcttcaatca ctacagaatt tgtcgcctgg caatcagaaa
    51181 gctgctgttc ttcataccaa atactctgtt ccaaatacac cgacacagac tgaagtccgt
    51241 catttgaaac cttgctgtca agctgagttt ccacccatcc atctctttgt tccttgagct
    51301 tggggctttc tatcttaatc ccatacaaat ccagatgcac cagatctgta atcaacaatt
    51361 ttgcttctcg gtaaataccg ctaccagagt accaacggct gctaggttgc tgattgtcca
    51421 caaaaactgc aagctggttg gccgtcccat cgtttctcag ataaggcgtg atatcataag
    51481 aaaagggcgt atagccattg ggataatagc ccagcacttg cccgttgata tagacttggg
    51541 cattcatgta aaccccgtca aagagtaaac gcaccgatac aagactggca tcttcttcca
    51601 agtaaaactg cgtccggtac caggcctgac ccccatttag ctgaccaccc tcattttgcg
    51661 ctggcgaata ctggtcaaaa tcattgtaaa tactccagtc atgtggcact tgaatagcct
    51721 gccaattttc cctctgaaaa tccggcgcca aggcctcttc tttccctacc tcttgactaa
    51781 aaaaccagtg atttcgtaaa acttcttctc ttctcataca agcattcctc tttaaacgac
    51841 tcgatttact tgactatagc acaaaacaaa agcgctttca atatttcctc catcacttcc
    51901 taaatactca gaaaagattc tataatttgt ggaaaagtct gaaaatcact ccatattcaa
    51961 gcaaactgta attttcaaga aacagggtca aaccttacaa gagcgcaaaa aacacctctc
    52021 tgaatgttca ttccagagag atgtcccttt tgtcttaact attgtaaatc gactgtctaa
    52081 agtcatctga atctttgaga taagctttat aaatcgcttt tttgagtttt tgaactggag
    52141 tttcaataaa ggtataggtg cgcgcagttg ttttgcctgc cttaatctgt ttcaactcct
    52201 cttgaacagc tttctgcatc aattctttta acttctgact tgtcagactt atttgctgac
    52261 cttcatattg gattgtcaaa ggtttcaatt gatcaatctt agctactctt tccttgaact
    52321 gtgctttttt gaactcagca tagctcttac cattcaaaat tttattgaaa atgtaagtat
    52381 cagatagagg cttgttctca gactctgcta cttgcttgta ttgatttgat acataaggta
    52441 caaatccttc ataatagccc aaagcagcca tcaattcaaa tgcttgtttt ctaaaggtga
    52501 tgtccccact cataccagag tcattttgac taacaccata aatggtatca aacatatcca
    52561 ctaaataata tccattagct tttacatcac ctgttgcata aaatccattt atgatataac
    52621 gattaactaa aatattatta tcaatcaagc tattgatatc cgttagtttt tccgcttctg
    52681 ccaatgtgat ttccgagatt ttttcactct tgtgcgtagc tttaaccgct gaatttgtat
    52741 atgttactgt cgctctaggt cccttcgacg caatcggtgt tatttttttg aaatatttag
    52801 ttttatcttc agctgtaagt tgttttgttg cttccgcttc taaataatct aaactatata
    52861 acacatcaaa acttccatgc atataagatt tcaagtctgc atctgttttg aatctatctg
    52921 gtgttttgtt gtagaagcca tttttcttac tttcatcgta aacgaagttt aagttaaaat
    52981 atgtatcttt ttctggtgtg taagaatttt caaagatacc tcgagcataa aactccgcag
    53041 ctgtaccatc tcgacgacca tgattattaa acagcactgt tctgtctaat aaatgcgtca
    53101 tttcgtgaga gtaagtcgct gagcctctat catccagaac tctatctatg aagtaacgga
    53161 cacctactcc attggcttct gcacctacat tttgcgatgg tgaatacagg cctagaggtg
    53221 tcataaagtc tttaacccct ttatctgctg ttggtcctga ttctttagac catgttgtcc
    53281 ctattccagc agacgtatcc gccgtgtatt ttttcatcgt gtctacaatc actctatttg
    53341 taactaattt agaacgattt tcttcttttg taattctgta taaagtatca acgtatgcag
    53401 cctgtcttgt cgcagtcaat tctatcaatg ctttaacttt ttctaattct tctttatact
    53461 taactggatt tgttttctct aaagaagtat ccatatacgt tccgatattt ccgtaggtca
    53521 ccgtcgctat gttagttata gcatacacgc ttggttcttt aatatttacc aaacctaata
    53581 atatagctac attttgttgt cttctacttt caatttgaag attttcagct ccatatttct
    53641 caggaggtgt agttaatttt gtatataatt ctactttttc gtcaggtttc tgtttcgaag
    53701 gcttttcaac tacaatagct ttagtcgctt tcttcaacca cgtatcgcca tccatattag
    53761 tgaatgtagt tctgttgtaa tctaggaact ctaataatgt tggtttatct gtaattttac
    53821 caaagtattt ctcaaatgtt tttggactat tggttaatct taattcatca taactcatag
    53881 acccaaggta tgttaaccaa tctaaagaag taaggtcttt tttaccgaac gagcttggat
    53941 tataagctag aatatcacgg atattggtat ctccaaaatc aatattataa aaacgattga
    54001 tataggataa ccctagtaat attttctctt tattagcttc aatatcacct tttacttttt
    54061 ctctattttg ttgattgtca cctaaaatac tcaaggtatg cgccgcaact tttttcaaac
    54121 tagcttctaa atcggtttta gtcgcatcga gtgtttcttt aatagataat ttttcaagat
    54181 attcttttcg tagaatttct tttacttctt catcagttat cgccggatta gtttttcgta
    54241 attcttttcg ttttaagaat aaatctcgat ttatttctat atctgatgga gctaactgcg
    54301 cttttaactt ctcatttgtc aacaagtcta catttctcat ctccgcagta gcttccgtta
    54361 cgtttactgc attttctaat tcgctattaa ttgggagtaa atgaggtttg tcttctttag
    54421 tgccaaccaa gattactttt tggcgcatag ctttagttgt ttcagtcttc tctactggat
    54481 ttgttaactc tcctgtctcc ggattaactt cataagtttt agtgacggtt gtttcccctt
    54541 gttcacctgc atcctcaacc tcttcttttc ctttcggaag ttcagggtta tcttttctca
    54601 actcagtgat ttctataggt gacactatct tttccacatt tccaactttt actattttgt
    54661 ctttagctgg aatattttct attgtagttt cttctgagat agctcctgtt tcttgattta
    54721 ctgtgtattt tgtagttgat actgtacgcc ctttttcacc tggtgtttct tcttgttgca
    54781 cattgtaact taaatctggc gatgctttat aaactgtctt aaaaggaatt tcttgtgatt
    54841 gaactgttgg ttttgttcct actgccgtta ttttcgcttg catttctttg gctacttttg
    54901 ttgaaacttc tggctccgtc aagtctcctg tttgtgcatt tactttataa acacgaatag
    54961 tagtcgtttc ttcgtcctga ccttgatttc tgacttcttt gacaccttta tcaagtgact
    55021 catccgcgac aaactgttca cctcttttgg ttgttgaggt tgtttcttct acatttccaa
    55081 ccttaacaac tctatctact ggatgttttt ctatctttgt tgaagtgttt tctactattt
    55141 tcccagtact ttcatcaaaa ctgtaagaag tagttgtcac ttccttacca ttttcgcctg
    55201 ctacttctac ttgttgctct ttgtaattta aagctggcga tgctgtatag actgttttat
    55261 agtccaatac tttttctttc gttactggac tcgggacagt tggttgcact tgtgaatctg
    55321 actgctcttg gggagctgag ggttcaggct gaacagctgg agctggttcg tttccttgac
    55381 ttgggttgac ctctggagat ggagccgttt gttctggttg tttttggggt tcttgtgggg
    55441 cagattcacc agcatctttt gccccctcaa ctggattctc cagctggttc tcctcaccct
    55501 tttgttccgc aggagattga tcttcctctc gtcctgatcc cacggctggg cttgctagct
    55561 ctggttgctt cggctcggct tcgggtttgt caggtgtttg tttctcgccc ggcgtttctt
    55621 ctgctcctgg tcttggttgt cctattccag cagctggttt tgtctctgtt tcattctcta
    55681 ctattggttt tttctcaatt ttttcaggtt gaactggttt aacttcctct ggctctggag
    55741 ttacaggttg tgttggtttt ggtgcaactt cttccttagt cccaactgca atcaccttgt
    55801 tctccggctc ttttgtgact atattttcta taactttgct agatttttcc ccgttaacga
    55861 tagtgacttc cgtatagatg gtgcgttcac caacaacacc ttcttgaaca actttagttt
    55921 gccctttagc taagcttgca tcttcttgtt ctatcgtttg gaaagcaatc tcctctgtag
    55981 taactagcaa ttctggtttc tctactgtgg catttaacac gccttcctca ggacttggtt
    56041 tataatcact attattctct gctttcggtt gttctacttt gggatttcct gcaatttctt
    56101 ccttagtccc tataggcaca atttcatcag gagcttgttt gcttacagga gcttcaacaa
    56161 ttttctcaac tgcgtaggat ggttgaggag attgcacaaa atcttcagtc gagctaaaat
    56221 gagatgcagt ctgtggtaca atcttcttag attgaggttc tgaaccgtcc tcctttttag
    56281 ataatgctgg cgactctacc ttatcaacag aaagttcctt gttactactg tctcttacca
    56341 aataatgact tgaaattagt gttgaactcg tttcggtagc ctgtacaggc tgagcatcca
    56401 taccagcaaa aaataaactc gttcctagca agaccgaaca agctcctatt gcatatttcc
    56461 tcaaagaaaa acgctgcttt cgctcaaatt gaaattcttt catcccatct cccatcattc
    56521 attattactg tatatttagt atatcagaaa tagtttgtat tcacaaatct ttctagttat
    56581 tcccttatca ttccaagtta agggcaacaa catacaataa tttttattta atactcttcg
    56641 aaaatctctt caaaccacgt cagcttccat ctgcaacctc aaaacagtgt tttgagcaac
    56701 ctgcggctag cttcctagtt tgctctttga ttttcattga gtataaatgt atatcaatgt
    56761 tctttgtttt tcttaacaaa cgcaacgcaa aaagagcctg ttgccaagct ctttagtcta
    56821 ttatttcttc tcagcgcgga gggctgacaa gatttgtgta cggatatcat ccacaccatt
    56881 tggcgtattt ggtaaaaaga tcgtttgatt tcctttagaa gcaaaggtat tcaaggtatc
    56941 caagtactgg ttggtcaaga gaatggacat gatttgttct tctgtcatac caacattggc
    57001 ttccttgagt tcggtgatag actctgccaa tccatccaca atcgccttac gttgttgggc
    57061 aatccccaca ccatgaagac gatctttttc tgcttcggct tcagctgcag tgacgatttt
    57121 aatcttgtcg gcttctgcca attcttgcgc tgcaacccgc ttacgttgcg ctgcattgat
    57181 ttcattcatg gattgcttaa cttctgcatc tggttcgacc ttggtaatca aggttttcac
    57241 gataatgtag ccataagttg tcatttcttc tgctacttgg tgttgaactt caagggcaat
    57301 ctcatctttt ttctcaaaca attcatccaa agttaatttg ggaacagaag agcgaagggc
    57361 gtcttcaata taagatttaa tctgagattc tggacgcatg agtttatagt aggcatctgt
    57421 cacgctctgc tcattgacac ggtactgagt cgctacattc atcataacga acacattgtc
    57481 cttggtctta gtctcaacca caatatcgct ttgcaacaaa cgcaactgaa tccgcgctgc
    57541 aatcgagtcg atcccaaaag gcaagcgaat atgaataccg ctattggcaa ccttttggta
    57601 tttcccaaag cgttcaataa tcgccaccga ctgctgacga accacataaa ctgtgctcag
    57661 tgtgactatc agcaatagga acacacaaac aatcagaaaa atcatgaaaa atattgccat
    57721 aatggaacct ccacaagtat ttttctagta ttatagcaca tttaaagaag actgtgcagt
    57781 ttttactgcg atttttcctg aaatgtcaat aattagaggt gaagccaaat ttcaagttac
    57841 taatctcttt ttttcactgc attaacttct atagaatctt catccatagt cagagaatga
    57901 cttgccttaa tttttctctc gtgctatact atttatgaaa ttaatgaata ggagatattt
    57961 catgaaatca gcaaaaacta ctgttacaat cctttctgca cttgcatctg tcgtcttatt
    58021 atctagttgt gcaacaaagt ctacagaaac acagacaagc aataagagct catctgataa
    58081 tcagattaca atgacatatg accaactacg gtcaagagaa aatactatgt caaccctttg
    58141 gtattaaaaa tcagctgaaa ccaaagcctt gtatctacaa ggttataatg ttgccacaga
    58201 tcgtctaaag gaattactga aaacagaatc agacaagcct tattcaatcg ttttgaactt
    58261 agatgaaaca gtcttagata atagtcctta tcaagttcaa aatatcaaag acggaacagc
    58321 attcacacct gaaaattggg atatttgggt tcaaaaagct gcagcaaaag ccgtcccagg
    58381 agctaaggag tttcttcaat ttgccaatca aaacggcatc caaatttact atatttctga
    58441 ccgctcagtt aatcaaatag atgctacgat taaaaatcta gaaaacgaag gaatccctgt
    58501 acaagggcgt gatcacctca tgctcttaga aagtggtgta aaatccaaag aagggcgtcg
    58561 ccaagcagtt caagagaaaa ctaatctagt attactattt ggtgataacc tcgtagactt
    58621 tgcagatttt tcgaagactt ctgaatctga ccgtgataaa caacttgaag aattgcaaaa
    58681 agaattcggt gaaaaattca tcatcttccc aaattcaatg tacggatcat gggaaacaac
    58741 tgtctacgct ggcaataaat tggatgctaa aggtcaagtg gaagaacgcc aaaaggcttt
    58801 gcaaggtttt aataaataaa acaaataagc tgattctata taaactaagg ctaacttaca
    58861 atcttcaaaa agtggatagg aaggaattct gataatcaga aaactttcct atccactttt
    58921 tgaattgata taacatcaag cttttataca tctgatacta tatgtttttt gattttaaag
    58981 actttttgcc tatcccctat atttcaagaa ttaagcaatc ttgatgatct aaatttgttg
    59041 tgatataata tttttgtcat cggttaccga ttacgttcct atgggttcgt gagaggaggt
    59101 gaaactaatg a
//
LOCUS       DAVKDZ010000005        46081 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000005 DAVKDZ010000000
VERSION     DAVKDZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46081
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.005"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(450..1955)
                     /gene="adcA"
                     /locus_tag="VWZ30_000288"
     CDS             complement(450..1955)
                     /gene="adcA"
                     /locus_tag="VWZ30_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEU9334096.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLFQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYVGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKFTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(1965..2771)
                     /locus_tag="VWZ30_000289"
     CDS             complement(1965..2771)
                     /locus_tag="VWZ30_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9334097.1"
                     /translation="MLSLLSYDFMQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFLLISLVRRFIK"
     gene            complement(2764..3468)
                     /locus_tag="VWZ30_000290"
     CDS             complement(2764..3468)
                     /locus_tag="VWZ30_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9334098.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVTISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENDQEVGHA"
     gene            complement(3468..3908)
                     /gene="adcR"
                     /locus_tag="VWZ30_000291"
     CDS             complement(3468..3908)
                     /gene="adcR"
                     /locus_tag="VWZ30_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEU9334099.1"
                     /translation="MRQLAKDIDAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQQFLTALVGEIK"
     gene            complement(4063..5331)
                     /gene="dltD"
                     /locus_tag="VWZ30_000292"
     CDS             complement(4063..5331)
                     /gene="dltD"
                     /locus_tag="VWZ30_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU9334100.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMRHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYEPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIGDVVKADAEKNTSNNDLGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFFKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            complement(5324..5563)
                     /gene="dltC"
                     /locus_tag="VWZ30_000293"
     CDS             complement(5324..5563)
                     /gene="dltC"
                     /locus_tag="VWZ30_000293"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEU9334101.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            complement(5577..6821)
                     /gene="dltB"
                     /locus_tag="VWZ30_000294"
     CDS             complement(5577..6821)
                     /gene="dltB"
                     /locus_tag="VWZ30_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEU9334102.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAAILPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILILLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGMIIELRDGVIKDFTLWKFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            complement(6818..8368)
                     /gene="dltA"
                     /locus_tag="VWZ30_000295"
     CDS             complement(6818..8368)
                     /gene="dltA"
                     /locus_tag="VWZ30_000295"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEU9334103.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQRHTYGDLKADSDS
                     LAAVIDQIGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMNFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            complement(8389..8520)
                     /locus_tag="VWZ30_000296"
     CDS             complement(8389..8520)
                     /locus_tag="VWZ30_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEU9334104.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            complement(8855..9559)
                     /locus_tag="VWZ30_000297"
     CDS             complement(8855..9559)
                     /locus_tag="VWZ30_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9334105.1"
                     /translation="MMSELFGEFLGTLILILLGNGVVAGVVLPKTKSNSAGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTVGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEVEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            complement(9627..11453)
                     /gene="glpO"
                     /locus_tag="VWZ30_000298"
     CDS             complement(9627..11453)
                     /gene="glpO"
                     /locus_tag="VWZ30_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEU9334106.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYEEDGATFSLFRLKVAMDLYDLLAGVNNTPAANKVLSKEEVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQIRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELTLSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRVDVEAALANNDLAELKN"
     gene            complement(11484..12992)
                     /gene="glpK"
                     /locus_tag="VWZ30_000299"
     CDS             complement(11484..12992)
                     /gene="glpK"
                     /locus_tag="VWZ30_000299"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU9334107.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAMLPEV
                     RSNSEIYGKTAPFHFYGGQVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWIRDGLRMVENSPESEKYA
                     RDSHNDDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDAQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            complement(13151..14578)
                     /locus_tag="VWZ30_000300"
     CDS             complement(13151..14578)
                     /locus_tag="VWZ30_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9334108.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKVLDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLC
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWSHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKAPSFFAGQHLPLGTEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQKMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFRGMAEEIFHTLPTFQQGTDLDKKILWEWLQLIEYMAEND
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYNLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            14743..15615
                     /gene="hslO"
                     /locus_tag="VWZ30_000301"
     CDS             14743..15615
                     /gene="hslO"
                     /locus_tag="VWZ30_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU9334109.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGSIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPVVGNGQFLVITDYGIGNPYTSLTPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKEEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            15602..16582
                     /gene="dusB"
                     /locus_tag="VWZ30_000302"
     CDS             15602..16582
                     /gene="dusB"
                     /locus_tag="VWZ30_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU9334110.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWANPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEGLLQLDKT"
     gene            complement(16648..17313)
                     /locus_tag="VWZ30_000303"
     CDS             complement(16648..17313)
                     /locus_tag="VWZ30_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334111.1"
                     /translation="MKKKTYIKLMVAIVLVSTLLLGACGNKKEATETSQSSSSKVASS
                     SKENKIQKSSSSISSEKSKVSSDQSEMATNQSQATQTSSAPESRQGQEDSSQQTPSTQ
                     QHSQAQSNQSTYDPTTDRKLQDKQAEHNKRYKSVLTMVDGDFSATAGNWKGANGETVT
                     VSSDGQFTVETVDGKKENYSIKGYSYTLDDGKYNVKVGDGKVIQITTGADGAVSSVSL
                     IQQ"
     gene            complement(17530..19045)
                     /locus_tag="VWZ30_000304"
                     /pseudo
     CDS             complement(17530..19045)
                     /locus_tag="VWZ30_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(19267..19578)
                     /locus_tag="VWZ30_000305"
     CDS             complement(19267..19578)
                     /locus_tag="VWZ30_000305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334112.1"
                     /translation="MKRIIKLTLTLAVSTLVLVGMANTVQADETNARVSTHQTMRKNP
                     FRLHFEKVVLEAYQAAAEMAKNNYDSYAQLDEEIRNAKEELTDGSRTLIEVASSTILF
                     R"
     gene            complement(19713..21989)
                     /locus_tag="VWZ30_000306"
     CDS             complement(19713..21989)
                     /locus_tag="VWZ30_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEU9334113.1"
                     /translation="MSKSNHERRMRYSIRKFSVGVASVAVASLFMGSVVHATENVSAN
                     PPIPQIVSPGDKKEYEDAVQRVNKEISDYVTSRLDSLDRSVSGFSEIVTKVQVVVDKY
                     RDKIDRVSTKSMVEELGREVKKKVDEEIKLFQNRSGSKSTPKELSLNDGLQGGGDPSV
                     GQGPGVVPQPGGQAGGSMVVPPVTQTPPSTSPSPGQKATEAEKKKLQDLIRQGQEELK
                     KLEDYLREVNNYPELPDNDPDYKVQKKDIWDNSKDTAPKKIQVFKEQLEKQTYTEKTL
                     KDAVAEFIYYQFHAQIETMTRKIATYRKKHPNVAEVERLFSEKLKQTANSTYATLEGE
                     ALKTYFERDFLPVFNKIHSIIEELEKKSSQGELKKQDKVAEAQKKVEEAEKKAKAQKE
                     EDRRNYPTNTSKTIELEIAEAQVEVAKAELELAQAQAQTPQDTDKINTAKAKVETAKS
                     KVKTLEKIKSDSGRAQAGDQKPSMPAPDTKPDLQPQPEVPSTSPEPKPIPQPDGKQPS
                     TPKEPENKKPSVPTQEKPIPQPEKPKPEVKPQPETPKTSKIITASDGKTKVTVVFDKA
                     VDADKVNIKEVTTKELAEKIARQTGGGTVRIFDLSLSKGGKETHVNGERTVRLALGQT
                     ASDVHVYHVKENGDLERIPSKVENGQVVFKTNHFSLFAIKTLSKNQNVTTPKQIKPSV
                     QHGQTQIGENQTGKFQNKEVNHKPLATGNETMAKENPTSATEKNLPSTGAATNLVLEI
                     IGLLGLAGTSLIAMKRRK"
     gene            complement(22072..22599)
                     /locus_tag="VWZ30_000307"
     CDS             complement(22072..22599)
                     /locus_tag="VWZ30_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEU9334114.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGQEFGVENTKRLT
                     FAHIIFYLSCFVEAMVHKTIFDGMSMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKFVDHFLFRTVKHPNYFLNILPELVGLALVSHAYVTFIFIFPIYATILYHRIAEEE
                     KLLQEVIIPNGRMRG"
     gene            complement(22708..24048)
                     /locus_tag="VWZ30_000308"
     CDS             complement(22708..24048)
                     /locus_tag="VWZ30_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9334115.1"
                     /translation="MIKNPKLLTKSFLRSFSLFGAIALIIHIAIYLTFPFYYIQQEGE
                     KFNESATIMVKYLEGKKIQELPSLLESYSKSLRRSAHLKDDVLDKRISLVHDLEIKEG
                     SPTSYIVTIDRQITTADGKKVTIQFIQGVDIYKEATRIMFLYLPYTFLATLVFAFLFS
                     YFYTKRLLEPLFYISKITSKMQELDHNIRFNEMRKDEVGEVGKQINDVYENLLRVIDE
                     SERRNERIVRLQNQKVSFVRGASHELKTPLASLRIILENMQYNIGDYKDHPKYIARSI
                     NTIDQMSHLLEEVLESSKFQEWTECRETLTVKTVLVDILSRYQELAHSRGVTIENQLT
                     DATRVSMSLKALDKVLTNLISNAIKYSDQNGCVMISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKAEGSGLGLYIVNNILESYQMDYSFLPYERGMEFKIDLQID"
     gene            complement(24045..24698)
                     /gene="bgrR"
                     /locus_tag="VWZ30_000309"
     CDS             complement(24045..24698)
                     /gene="bgrR"
                     /locus_tag="VWZ30_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor BgrR"
                     /protein_id="HEU9334116.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIVAKDGQEALEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINRNHDIPVIVLSALGDEETQMQVFDLYADDHVTKPF
                     SLVVLVKRIQALLRRYYVVEDIWHYQDVTVDFTSYQARVKNEEVAIKPKELLVLKCLI
                     QHKNQILSRKQILESISNDFADLPLDRVVDVYIRNLRKKLDLDCIVTVKNVGYKISL"
     gene            complement(24953..27385)
                     /locus_tag="VWZ30_000310"
     CDS             complement(24953..27385)
                     /locus_tag="VWZ30_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9334117.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQAHSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGELVAGKTLKI
                     GVKADQLKFDIA"
     gene            complement(27387..27845)
                     /locus_tag="VWZ30_000311"
     CDS             complement(27387..27845)
                     /locus_tag="VWZ30_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEU9334118.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            complement(27964..28692)
                     /locus_tag="VWZ30_000312"
     CDS             complement(27964..28692)
                     /locus_tag="VWZ30_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9334119.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENLKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            complement(28692..29699)
                     /locus_tag="VWZ30_000313"
     CDS             complement(28692..29699)
                     /locus_tag="VWZ30_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9334120.1"
                     /translation="MKKTWKVFLTVLTALVAVVLVACGQGTASKDNKEAELKKIDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            complement(29738..30496)
                     /locus_tag="VWZ30_000314"
     CDS             complement(29738..30496)
                     /locus_tag="VWZ30_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9334121.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            complement(30459..30749)
                     /locus_tag="VWZ30_000315"
     CDS             complement(30459..30749)
                     /locus_tag="VWZ30_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU9334122.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     regulatory      complement(30820..30922)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(31005..32351)
                     /gene="cbpD"
                     /locus_tag="VWZ30_000316"
     CDS             complement(31005..32351)
                     /gene="cbpD"
                     /locus_tag="VWZ30_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEU9334123.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     gene            complement(32440..32706)
                     /locus_tag="VWZ30_000317"
     CDS             complement(32440..32706)
                     /locus_tag="VWZ30_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334124.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            complement(32708..34060)
                     /gene="dnaB"
                     /locus_tag="VWZ30_000318"
     CDS             complement(32708..34060)
                     /gene="dnaB"
                     /locus_tag="VWZ30_000318"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU9334125.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            complement(34104..34556)
                     /gene="rplI"
                     /locus_tag="VWZ30_000319"
     CDS             complement(34104..34556)
                     /gene="rplI"
                     /locus_tag="VWZ30_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU9334126.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            complement(34553..36526)
                     /locus_tag="VWZ30_000320"
     CDS             complement(34553..36526)
                     /locus_tag="VWZ30_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU9334127.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTSLLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDHVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            complement(36662..37210)
                     /gene="raiA"
                     /locus_tag="VWZ30_000321"
     CDS             complement(36662..37210)
                     /gene="raiA"
                     /locus_tag="VWZ30_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU9334128.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            complement(37290..37952)
                     /locus_tag="VWZ30_000322"
     CDS             complement(37290..37952)
                     /locus_tag="VWZ30_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU9334129.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQFWCKEGVGVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            complement(37949..39247)
                     /locus_tag="VWZ30_000323"
     CDS             complement(37949..39247)
                     /locus_tag="VWZ30_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9334130.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            39303..39938
                     /locus_tag="VWZ30_000324"
     CDS             39303..39938
                     /locus_tag="VWZ30_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU9334131.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYASSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            40223..41143
                     /gene="cysK"
                     /locus_tag="VWZ30_000325"
     CDS             40223..41143
                     /gene="cysK"
                     /locus_tag="VWZ30_000325"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU9334132.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(41340..41738)
                     /locus_tag="VWZ30_000326"
     CDS             complement(41340..41738)
                     /locus_tag="VWZ30_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9334133.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNRHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            41729..42037
                     /locus_tag="VWZ30_000327"
                     /pseudo
     CDS             41729..42037
                     /locus_tag="VWZ30_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(42178..42501)
                     /gene="tnpB"
                     /locus_tag="VWZ30_000328"
     CDS             complement(42178..42501)
                     /gene="tnpB"
                     /locus_tag="VWZ30_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9334134.1"
                     /translation="MMIQLSDLGQVHIVCGKTDMRQGIDSLAYVVKTHFELDSFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGKLTWLSTEKDVKALTSEQVDWLMKGFS
                     ITPQI"
     gene            complement(42747..43787)
                     /locus_tag="VWZ30_000329"
     CDS             complement(42747..43787)
                     /locus_tag="VWZ30_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU9334135.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            complement(43866..44645)
                     /gene="rpsB"
                     /locus_tag="VWZ30_000330"
     CDS             complement(43866..44645)
                     /gene="rpsB"
                     /locus_tag="VWZ30_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU9334136.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            complement(44869..46047)
                     /locus_tag="VWZ30_000331"
     CDS             complement(44869..46047)
                     /locus_tag="VWZ30_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU9334137.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
BASE COUNT        13296 a        10267 c         8379 g        14139 t
ORIGIN      
        1 tttcaagcat taagtaaggt tgatggtcta aatttattgt ggtatactat ttttgtcatc
       61 ggttaccgat tacgttcctt acggttcgtg agaggaggtg aaactaatga accttatcat
      121 agagctcgct cttactatta tagcggacgt catcgcagga attatcttgt atttcgtctg
      181 caaatgacta gatggtaaga agtagaccga ctgactagcc tatcaacacc cgttaaatcg
      241 ctaagatacg tcaaaaaagc ccttaactac ggcaatagtt aggggctttg gtgttctaat
      301 gaaccttgct tatcaagttt attctaacat acaggcccag atatttcaag agttttattt
      361 attttttaca gttctgtaag gtctatagtg aagttagcca tctagtaccc aaaaaccgac
      421 tagctcttat gaactagccg gttttctcat caatgtgcca acatttcttg ggcgatttct
      481 tggccagata ggttatctgg gtagtaggtt ggccagttat ccatttcttc aaagagggct
      541 tcttggcttg tgcctccaaa gaagatatgg aaatgttctg ccttaactgg ggcgatattg
      601 tggtcactaa actgaacata tttgaattgt ccagcgtcag catctgtggc ttcaaagagg
      661 aagcgaacac cacgattgcc tttcttgtaa gtcaagattt tcttaccaac atacttgtag
      721 gtgaatttct tgctttgtcc accttgaaca aattccatag tattatcagt aatattaatc
      781 ttagtcacat ctgtctgata gccttttgta tagtaagcct tgtactcagc ctgggtcatc
      841 ttaccagtca acttagcctt gtagtcaaag acttggtcaa acgtgccatc ttcaaggaaa
      901 ggataaactg attgccagtt acctacatag tcactcaagg tgcgatcctt gacagctgca
      961 tcctcaaagt aaccattttg aactgtcttg gtatcctctg ccttttcagg ctcgattgct
     1021 gggccttctt ggtccgttgt ttgtttcaga gccttgaggt ttttctccat cacggaaatg
     1081 tagttttcgc cagccttggt atcctcttct gtcagacttt ccaaaggatt gaggacatct
     1141 gttttgacac ctgcttcttt tgaaagtgtg ttagcaaggg cttgtgaagc attttcttca
     1201 aagtagatat aggcaatctt atttttcttg acgtactctg tcaattccgc caagcgagca
     1261 gctgatggct ctgcatctgg agagaggcct gaaattgcga cttgtttgag tccatagtct
     1321 aaggcaaggt agttaaaggc tgcgtgttga gtcacaaagc tcttttgttt ggcttgaaac
     1381 aagccttctg cataagcctt atccaaggct tgcaattttt caatataggc agcagcattc
     1441 ttctcaaagg tctctttttt atcaggataa tctgctgaca agctatcacg gatatgctct
     1501 actagtttaa tggcacgaac tggtgataac caaacatggg gatcgaactc atggtgatgg
     1561 ccttcttctc catggtcatg gtctccctct tcttcctcac cacctggtaa gagcaacata
     1621 tcccctgttg ccttgatggt tttgaccttt ttcttatcca aagtatctag caatttaggt
     1681 acccatgttt ccatgttttc attttcataa acaaaggtgt ctgcgtcttg gattttggca
     1741 actgccttgg cagacggttc atattcatgg ggttctgtac cagcaccgat taggagttct
     1801 acattagcag tatctcctgc aacttgcttg gtaaattcat agacagggta aaaggttgtc
     1861 acgatattta gtttgccatc tgcctgcttt tgattggaac aagccactaa aaacaaggca
     1921 catagactgg ctagcaataa gctaattttt ttcatgatat tctcctattt gataaaacgt
     1981 cttactaaac tgattagcaa gaaaacagtt acaaaaataa tggtaatact tgcacttgca
     2041 ggtgtttctg catagtagga aatgtaaagc cctgctacca ttcccaaaaa tccaatagca
     2101 ctggcaagca gcataaccga tttaaagttt ttccccagac gcagggcaat actagctggc
     2161 aagaccatga tagtcgatac cagaagagct cccgcagctg gaatcataag ggcaatggcc
     2221 acccctgtca ccatgttaaa aagaatggac atggtacgaa ctggcaagcc atccacaaag
     2281 gccgtatctt cgtcaaaagt taagatatac ataggacgaa gaaagagaaa ggtcaaaatc
     2341 aaaacaaccg ccgcaatgac aaagagggaa atgacctgtt cttcactgat agtcacgatc
     2401 gaaccaaaga gatattggtc caaactcatt gaactagagc ttttaccctt actcatgaca
     2461 atcagagaaa cagccagacc tgttgacatg agaatagctg tcccgatttc cataaagctc
     2521 ttgtaaaccg tacggagata ctccagaaag accgccgcaa tcaagacaat agcaatcgta
     2581 gaaacagttg gcgaaatccc caaaaccaac ccaaaagcta cacctgaaag tgagacatga
     2641 ctgagggtat cactcatcaa actctgacga cgcaagatga ggaaggttcc aagaactggc
     2701 gagaaaagac tcatggcaat aacagccaga aaggcacgct gcataaagtc ataagataat
     2761 aaactaagca tggcccacct cctgatcatt ctcatgaacg ttgaaacaac gccatggcga
     2821 gtcttggtta cgaactagat gaatattgcg atccgcatag tccttcactt cttcagggtc
     2881 atgggtaatc atcaaaacag ccttgccatg atgatgggcg ctgtggtgca tgagttcgta
     2941 aaattcattt ttacttcctg catccatacc cgttgtcggc tcatctagga taaacacatc
     3001 tgggtcagaa gcaaacatac gcgcaatcac cgctcgctgc ttttgccctc cagaaagaga
     3061 ccccaagcgt ttgtctcgat gttcccacat gccaactgag tccagactgg ccttgatatg
     3121 ctcctcatca tgagcattca aacgacggaa ccatcctttt ctcggatagc gacccgactt
     3181 gacaaattca tagaccgtac ttggaaaacc agcattaaaa ctggcaattt gttgaggaag
     3241 ataggctatt ctcaatttct taccttgcgt atttgtcttt gaaatagtca cctttccaat
     3301 gcgtggttgc agaattccaa gactggcctt gatgagcgtc gtcttagccg ctccattttc
     3361 cccagtcaag gtaacaaatt ccccactatc aacactataa ttgatatgtt caagaacagg
     3421 ctccttatca taatagaagg acaaatcctc taccgtaata tatctcatta tttgatttct
     3481 cctactaaag cagtcaaaaa ctgctgaatc actttttgtt catttggagt aaactgagtc
     3541 gccacttgtt cataggttaa aagtgtatgc tcatgatgat ggtggtgctc ctcagcgatt
     3601 ggacgagcca agtcagtcaa ctgataaaaa atcacacgcg catctttaga atctttagat
     3661 gtttccaaca tcccttcctt gaccaaagac ttaatggcct tggtaactgc cgcctgactg
     3721 acattgagac gacgggccaa ttctgaattt gttaaagatt cctctgacaa gagcataagg
     3781 atatgctcct gggtatttgt cagagccacc tcgctagtgc aatgacctat taggatttca
     3841 tgctgatttt ccgcctgcaa aatcacctca ttcaaaaaag catcgatatc ctttgctagc
     3901 tgtctcatat ctgactcctt tccttttaga cttctctttt ttaagagaaa aatactattc
     3961 tttgacattt tatttaccag ttaattatat cacaagcaaa aaaagagtca agaaaaaacg
     4021 tgaaaactag tttcattctt gaactcttct atattatatt atctattgaa attctttgac
     4081 atctccatca taagtcgccc aatctttgct gaaaaagcgc tcattcagat gataagtcgg
     4141 agctggtgtg ggattggata ggaaaggatc aactgccttg tcaaaagcca accaacccaa
     4201 ccaaccaagg tgaatggtgt ccttcataaa gaaaggctcc ccgccgtcct tagaaaaatc
     4261 tgctatattg gtaaaacctt gactttctaa ctggtagcga atcttctgca ccgtttgttg
     4321 gtacatatcc tctcgtagac cagcatagtc catccatttt ttattaacag gtggaatgat
     4381 aaaaatcggg tttaccttag atttaaaaaa ctgtgttaaa accaactgca agtcattata
     4441 ctctggcgac ttgagatagg taaagctttt ctgagaatcc tttaatttct tcaaatcctt
     4501 cttgatctgc tcattataga aataattttc cattcccaga tcattattgg aagtattttt
     4561 ttcagcatct gctttgacaa catctcctat tgcttgataa gaaaactggt ctggcaagat
     4621 ttttaaatac ttagctacat gcttatcgta gttaacatag cctctaaccg aaaactgacc
     4681 aaaaaaggag gcttggcgtt cattaaaacg agccaataat tcaatcattt cattgtctgc
     4741 tgtcgacaat tcttctttac ttgccaactt ctgaaccagg tccttcatag ctacgtttgg
     4801 gaactgttgc agtaagcgag tcgctgcata ttgactagcc tgatccccag attgatgttt
     4861 cagaaaacta gtcaactggt ctccattaaa atactgctgg aaggctgctg gctcatagcc
     4921 atttttactg aaccactgag gtgagataac atacacaact tgtttattct ccagctgtgg
     4981 tagcatctgt tgcattccaa aatattggtt aagcgatgca gctcccccct gtcctaaaag
     5041 ataaggacgg taggaacgat tgtatttctc agctaatacc gcaggatgag caccgtcaaa
     5101 acgaagccat tcactagagc caaagaaggg aacaaaacgc acatttggat cagatagtgc
     5161 tctgactttt tgacttcgct ccttaaaact atcgatagta gtagccaccg ctgaacgctt
     5221 ttcagctcct agattatgac gcatctcagt aggataaaag aaaatgagca gaaaaaccaa
     5281 caaaccagcg atcaagaccg gtccgaagat catccataag cgtttaagca ttttgtagct
     5341 ccacaatacc agctatgatt ttattagctg tattccagtc gtcacgacca aactctgtta
     5401 cagggacacg aatgtcaaaa cggttctcaa tctccacaat caactcaacc gttcccatac
     5461 tatccaagac acctgcatca aaaagatctt catccatcat gtcagaaaca tcttccataa
     5521 acaactcatc aataatttcg ataacttctg atttgatatc catattttat ttccttttat
     5581 tttttaaacc atagattatt caagaatcca gaaaagatta agaatgacaa catgacaaca
     5641 tggaaagtga caaccatacc aagcaactga atccagcgat tctcaggtag ggcagccttc
     5701 cctgcttttt tccgttcctt attgagcgtt tttttcttgc gaacccaggc atcattgata
     5761 accaagccta gtccatgaaa gagtccatag gcgatatagt accaggtcac gccatgccaa
     5821 aatcccataa tcagcatatt tacaatgtag gccatgcttg aggttacatt acgattttta
     5881 aagactttct ttctggttaa caccatcacc attcgcataa agacaaagtc gcggaaccag
     5941 aaggacagac tcatatgcca gcgattccaa aactccttta aatcccttga taaaaagggc
     6001 ttgttaaagt ttatagggct acggattccc atcaagtttg agatggccaa agcaaacata
     6061 gaataacctg caaagtcaaa gaagagctcc agaccaaaag tatacataac tgccaaggca
     6121 tagagattaa agaagccacc tgactgcaag gctaaattct tcagaggagg tagtaaggtc
     6181 tctcctaaaa catgagctag gataaactta tacaaaaagc cccgcatgat atagcggaca
     6241 gattcatcca gcatatccat caactcatct cgctcaggaa tggcctgata attttcatta
     6301 aatcgcttaa agcgatcgat tggaccactc gagaaagttg gcatgaatag aaggaaacgg
     6361 aggaatttcc agagggtaaa atccttaatc actccatctc tcagctcgat gatcatccca
     6421 accgaacgaa aggtcaggta agaaattccc aagaacccaa gcaaagactg cgttccattg
     6481 atagctggtt gcaccttgac aaagataatc ggaagtaggg acagaaaact aactaagtag
     6541 aagacccact tgccatcctt gctttttcga taatgcttgt agaaaagcag gatcaatatt
     6601 tcccagcaaa ggtaaatacc caaggcagct agttgattgg tctttccacc caccaacatg
     6661 gtgacaataa agaagagact taccaacact tcataccagg caaagcgttt cttgaaaaag
     6721 aggcctataa agatgggcaa gattgcagca atcacataaa caaaatactg aggattgcca
     6781 tatggctcta aatgaggaag ctgttgaaaa aactccatca tctcttattc acctcgttaa
     6841 tcaatccttt gatgtcaatc tttccatttg gagttagtgg caaactgtct cggtaaagga
     6901 atttagatgg catcatgtag gacatcatga tgtctgtcag gtcttccttg atggccttgg
     6961 taatatcgat atctcgctca aactgctcac gaacaccgtc ttttaagatg acataagcca
     7021 atagattttg taccttgtgg tccttgttat agcgcggtac tgcgacagca gattcgataa
     7081 agcgagactt gttgaggttt tgagagacat cttctaactc aatgcggtaa ccgttaaact
     7141 taatctggaa gttcatgcgt ccgccgtaga gaagcaagcc ctcatctgtc atggttccca
     7201 catcgcctgt gtgataggct ggcagacctt caaactcaaa gaaggcttct gctgtttttt
     7261 caggattgtt catataacct tttgaaacag ctggcccaga aacaatgatt tctccctgtt
     7321 cgccatttgg cagtttattt ccttcctcgt caataataaa ggttggagaa tcagccttgg
     7381 tatagccgat tggtaggcgt ttgagagtcg ctaacatctc gtctgtcacg gcaactgctg
     7441 acagagctac tgtcgcttct gttgggccgt aagcattgat gatacgggca tttgggaaac
     7501 gctcgcgcag tttttgagct gttttgaccg tcaattcttc accatcaaag tagaaatgcg
     7561 tgattccagg cattttctca ctgttgaagt attcagacaa catggccata tctgcaaagg
     7621 atggtgttga tgtccagata gcgattggca atgaaaagat agccgcaaag agttgcttaa
     7681 agtcctgagt gatgactgaa ggaagagtga aaagcgtacc accaagtgcc aaggtcggtg
     7741 cccaatacat gacagacaag tcaaaagaat aaggtggctg tgccagcatt tgcggacgac
     7801 tcggtgtcgc aaattcctta tccgtaatca tccagtttgt aaagctgagg agattatcat
     7861 gtgaaatctg cactccctta ggcttaccag tcgtaccaga agtaaagata atgtagtaat
     7921 tatcatctcc cttgactgga tgcgtgattt catagttgtt cccttgggca aaggcttctt
     7981 gaacctgagc tagatttatc attggtgtag aaacctgctc caagggaaag gctgaaatgg
     8041 caataatcaa gcttggctct gctacttcta aaatagctga aactcgctcc aaggccgaat
     8101 ggctatcaat tggaatgtag gcatgacctg acttagtcag cgctacaaag gttgccaaca
     8161 tttcatattc ttggccacca aaaacaacca caggagactt ctcaggcaag cctatttggt
     8221 caatgactgc agccaaacta tccgaatcag cctttaaatc gccataagtg tgtctctgcc
     8281 ccaaaacatt atagacagga tagctaggct gtgtctgagc aaaatgctca atggtttcaa
     8341 tcatatctgc tattggttta tttgacacaa tagggattct ccttcaagtt aaaattcatt
     8401 atagataaag cttccttgac cctgaccaag atagctaaag aagtaaagca gccctagaaa
     8461 gataagaaaa tacaaggctg tccgaccaag aaagaggtac aattcttttc tctgtttcat
     8521 caagaaaaac cattcatttc tgtaattttt cgctaaaata agagtgattc ttactagctt
     8581 atttttctac cattgtacca ctttatatag tatcttttca attgtttacc gtatgtttcc
     8641 aatagatttc agcttatttt aaggattatg cggtttttct atgtctattt caaaagaaaa
     8701 aagcctgaga ttttcatctc aagctctatc aaaataattg ttttctaata ctcaatgaaa
     8761 atcaaagagc aaactaggaa gctagccgca agctgtactt gagtacggta aggcgacgct
     8821 gacgtggttt gaagagattt tcgaagagta taaactagaa aagtgagaat acaagcacgg
     8881 ccaaggctgc tccgataaca gggcctacaa caggaatcca agcgtaagac cagtctccgt
     8941 ctcccttgtt aggaattggc aagatgctgt gcatgatacg aggtccaagg tcacgagctg
     9001 ggttcaaggc ataacctgtt gtcccaccta gtgatagacc gataccgaca atcaaagttc
     9061 ccactgcaaa ggttccgata cctgcctgaa aatcgtaaag acccaaagca aagattgtca
     9121 acaccaaaac aaaagttcca aggatttcgc taatcaagtt tgatacagta tccttgatgg
     9181 ctggtccagt actgaaggtt gccaggatat tgcctgcatt ttcttctacc tcataatgcg
     9241 gtttgaattg caaccaaacc aaaatctgac ccagcatagc ccctgcgaac tgggctaaga
     9301 tataaggcaa aacggaagcc caaggcaagt caccttttaa agctacacca actgtcacag
     9361 ctggatttaa atgagctggg ctgagttttc cagatacaaa tactgcaacc gcaactgcaa
     9421 tcccccaacc catagtaatc acaatccaac ctgcactgtt gctcttggtt tttggaagaa
     9481 ccacacctgc aacaacacca tttcctagaa gaatcaggat taaagtccct aaaaattctc
     9541 caaataattc actcatcatt tttctgtctc cattaaaaaa ggggcgggcg acaaggattg
     9601 ctaccctcca cctcttttat tttttcttaa ttttttaatt ctgctaaatc gttgttagca
     9661 agagctgctt cgacatcaac acggtaagtt gctttttctt cttctgtcca gtcatagaat
     9721 cgtcccattt catccaaaac tggttcaacg atactatcca agctatcacg cataaagagc
     9781 atgtgattgg tacgacgaag aaggaagtca actggactaa gagtcaactc attgcgcatt
     9841 gcatagtgaa gggacaaggt gtctgccaag ctaagacctg gtgcttgttc caagctgtga
     9901 gcaagtgcaa agaccttcgg tgcatttgaa ccgtaaagat ttgctaggta atgagcttcc
     9961 ttgctatcca aaccacgtga aactccaagt tgcgcaaagg cttcgatttc tgaatccaca
    10021 tttgctgggt tcaattctcc acctgaaaca gggtaagtct tagagttgat gagtttaaag
    10081 ctgcggtcaa attctgcttt gaggatgtca accacgcgct ccatagctcc ttcagccatc
    10141 ttacggtagt ctgtgatttt accaccagca agagtcaaaa gaccattgtc atcacggtcc
    10201 aagctagacc cacgagaaac tgcagatgga tccaaatgtt tctcagatgt gctactttca
    10261 agcttgctga cagcagactc aacatcttca cgtgtttttt ctttggagag ataagattca
    10321 acagtcgcaa tcaagttgtc aaagctttca tcactgatgg taccgttatt tccaccatta
    10381 tagtcagagg cactgttccc tgcaatcaat ggacgaagac ctgcccagct gctttcgata
    10441 tcatcaatgg tgatgttgga ttctgggaag cggttgttga caatgccaag tagataatct
    10501 acatcttctt gagttacttt tggatgctcc aaatcacctg tgtagtctgt atcagttgta
    10561 ccaaagtaag tcttgttttc acgtgggaga acaaagacca tacgaccgtc acccaaacct
    10621 gtgtcgaagt aaactggctg tgaaaccttg attttgcttg aatccactac caagtgaact
    10681 cccttagttg gacgtatttg tgagaattgc gttcccttat tagacaaatt gcgtacttta
    10741 tcgctccaag gacctgttgt attgataacc agacgagcct tgatttcaaa gacttggtct
    10801 gtcaagagat cacgagctac aacacctgta atcttgccac tttcgtcaaa gaggaagcct
    10861 tctgctttaa cgtggtttgc aatgagagca ccgtcttggt tggcacgttt gatgttttca
    10921 atcacaagac gcgcatcgtt gttacggaag tcaaggtaaa ccccacctcc taccaaacct
    10981 tctttcttca agtttggctg gcgttccaag acttcttcct tgctcaagac cttgtttgca
    11041 gctggtgtat tgttgacacc tgccaaaagg tcatacaaat ccatagctac tttaagacgg
    11101 aaaaggctaa aggtagctcc atcttcttca taaactggca aaagcattgg gtctggtttt
    11161 ggaatgtgtg gtgcgatttg ttgaaccact gcacgttcag aaaccgtatc tgagaccact
    11221 tctacgtcga attgtttgag gtaacgcaga cctccgtgaa ccaattttgt tgaacggctg
    11281 gatgttcctt ctgcgaagtc ctgcatttca atcaaaccag tctcaagacc gctagctgcc
    11341 gcctgcaagg ctacaccagc ccctgtgatt cctccaccga taatcaagag gtccagggta
    11401 cgttcctgca tttttttaat tgacaattca cgtgttttct ttgaaaattc catacttaca
    11461 cactttctaa ataaatcgct ttattagtcg tctacttccg caaagacttg cgttgctttt
    11521 acagccttct tccagccctt gtagagttgt tccttgcgag attcgttcat agatggttca
    11581 aagagttctc ctgtctcatt caagagtttc aactcatcca aatccttcca atagcccacc
    11641 gacaaacctg ctaggaaggc tgcccctaga gctgttgttt ccaagttttt agcgcgtgcg
    11701 atgtcgattc ccaaaatgtc agcttggaac tgcatgagga agttgttcat agctgctcca
    11761 ccatccacct tcaatacttg aatagctgtc tgcgcatcca cttgcatggt gtcgatgatg
    11821 tcacgtactt gataagcgat agattgcaaa gtcgccttga taaagtcttc tttgcttgtt
    11881 ccacgagtca aaccaaagac ggaaccacga gcattttggt tccagtatgg agctcctaga
    11941 cctgtaaagg ctggcacgac ataaacttca tcatcattgt gagaatcacg agcgtatttt
    12001 tcagattctg gtgaattttc aaccatgcga agaccgtcac gaatccactg aatagcactt
    12061 cctgcgatga atattgaacc ttccaaggca tagtaaacct tgccgttgat tccgtaacca
    12121 atcgttgtca agaggttgtt ttcagacaac tgcatttctt ccccagtatt catgatgata
    12181 aaagaacctg ttccataagt atttttaacc ataccaggtt caaaagccaa ctgtccaaag
    12241 agggctgctt gttggtcccc agccatacct gagattggca cttgtccacc gtagaaatgg
    12301 aatggagctg tcttaccgta aatttcagag ttagaacgaa cttctggaag catagcctta
    12361 ggaatgttga ggatttccaa aatctcgtca tcccatttga gttccttaat gttataaagc
    12421 atggtacggg ctgcatttga gtagtccgtc acgtgagccg caccgtcagt caatttccaa
    12481 accaaccaag tatcgatggt accaaagagc aattctcctt tttctgctcg ctcttgagcg
    12541 ccctctacat ggtccaaaat ccaacgaacc ttggtagcag agaagtaagc atcaatgatc
    12601 aaaccagtct tttcatggaa tttttccaca taaccttgac tttttagttg ctcagccaaa
    12661 ggtgctgtct ggcgtgactg ccaaacgata gcgttgtaga tagggagccc tgttttttta
    12721 tcccagacga ccgttgtttc acgctggttg gtaatcccga ttgcttcgat ttgatttggc
    12781 ttgacaccac tttcgatgaa agctccagcg ataactgact gaacagagtt ccaaatttca
    12841 ttggcattgt gctcaaccca acctgcctga gggaaaatct gggtaaactc tttttgactc
    12901 gagctaacct tttccccttt tttgttgaaa atgatggcac gagaacttgt agttccctgg
    12961 tcaatggcca tgatgtattt ttcttgtgac atgtactgtc ctcctgataa agatcttgag
    13021 gtgtttcctc tttacactta ctagtataca aaataaagcg ctttcttatt tatcaacttt
    13081 ttttaatttt taaaaaaatg tgaggagatt gtgtaaattt cacaaaaaag acctggaaca
    13141 accaagcctt ttaagtgaat aataactgac gaatccctgc caaatcctcc atatccaagt
    13201 cattttttaa ataatagact ggtgtctgtt ccttcttatg aatagggtta ttggtaacca
    13261 gcaaattata atgccgacta gggtcataag cttcaatggt aataaaacga ttgtattcca
    13321 aataccgttt caaaatggct gccatccttg aaaagacaag aaaacctgat gtcaaatcca
    13381 aaccaatccg catatgctgg ccatcatttt cagccatata ctctatcaac tgaagccatt
    13441 cccagagaat cttcttatct aagtctgtcc cctgttgaaa agtgggcaag gtatgaaaaa
    13501 tctcctctgc cattcctcta aaatcatgtt ccatcagtaa atatggatgg cgattctcta
    13561 actggtactt gtagcggtcc tgtaaaatat agcccttata gagatagact tgagcacaaa
    13621 gctgactgag ttcataggta acgtgatcct ctgtataatc acctagcaac tcctccttct
    13681 tcattttttg aatcaattgc gtcagcaaat cggctaactg ccctccaaaa ccaaggatat
    13741 actccatagt atgaagagga agaatgcgat gagataggag gaaagagaag aatatcatca
    13801 tctcaccatc ctcagttcct agaggaaggt gttgcccagc aaaaaaggac ggagcctttc
    13861 tcagcaaacg aaggtagaaa atattgtcaa aataccctcg cattttctct tctatgacct
    13921 gaaactgaca ggcgttgacc cgaagacgtt gttgactgat gtgagaccag agaaccaagt
    13981 ccaatttctg ccccgcagac aaacttgcac cgcagatttc ctctaaattg gcaatctcct
    14041 gttttctctc tggtttctgc atgtgacctt cccattcctg actcgaccag acctttcgga
    14101 aaagacagaa atagaaatag cgaatctgat gttctggacc tcgccaacgt ccattttgga
    14161 tggataaatc aaattctgac aaaatctgat ttaaaccagc caagtgacga ccaagcgtag
    14221 cctcgctaat caccaattct tgagccagct gatgggctaa aaactgttgg tggcagagaa
    14281 gataaaccaa aatttggtat ttaacagcac tctccaaaaa caagctccga atatctctcc
    14341 ccttggtagc tgcaccgata gacagacgca gattttcatc ttctgagtga atagtcagtt
    14401 ctaagccact atccaaaacc ttgtcattga gcagggtgac atatttggtt agggttgctt
    14461 ttgaaaatcc tgtttcttcc attacagcct tgacggtcgt ctgagactct tgtaatagaa
    14521 aggaaaggat tgaaaattga ccacactcgg ctttctccat caaatctcct aaatacattt
    14581 gttacccctt tcattttcta ccttaagcat agcataaatc ttacaaatgc tgaaataatc
    14641 tgtttctctt tttattttca tgctgatttc ctggtccatt atcctgaaaa tcagtaaaca
    14701 cacggctccc cctttgggca tttttatgct aaaatagtag ctatggataa aattattaaa
    14761 actatatcag aaagcggagc ctttcgtgct tttgtccttg atagcacgga aaccgtccgc
    14821 actgctcaag aaaaacatca aacccaagct agctcaactg tagcgcttgg tcgaactctt
    14881 atcgccagcc agattctcgc agccaatgaa aaaggaaata ccaaacttac agttaaggtg
    14941 ttgggatcta gctctctagg ttctatcatc accgtcgctg ataccaaggg gaatgtcaaa
    15001 ggttacgttc aaaatcctgg tgttgacatt aaaaagaccg caactggtga agttctagtc
    15061 ggacctgttg ttggaaatgg gcaattcctc gttatcacag actacggcat tggaaatcct
    15121 tatacttcct tgaccccact catctctgga gagattgggg aagaccttgc tttctaccta
    15181 acagaaagcc aacaaacccc ttctgcagtc ggcctcaatg tccttttgga cgaagaagac
    15241 aaggtcaagg ttgcaggtgg tttcctagtt caagtcttgc cgggagccaa ggaagaagag
    15301 attgctcgct ttgaaaaacg catccaagaa atgccagcta tctctactct tctcgaaagc
    15361 gacgaccata tcgaagccct cctcaaggct atctacgggg acgaagccta caagcgtctt
    15421 tctgaagaag aaatccgttt ccaatgtgac tgtagccatg aacgctttat gaacgctctt
    15481 gccagccttc caagctcaga cttacaggaa atgaaagagg aagaccacgg ggcagaaatc
    15541 acttgtcaat tctgccaaac tacttacaac tttgatgaaa aggacctgga ggaactcatt
    15601 cgtgacaaat cttaatacac cttttatgat tggcaatatt gagattccca atcgtaccgt
    15661 tttagcgcct atggctggcg tgaccaactc agcctttcgt accatcgcaa aagagctcgg
    15721 agctggactc gttgtaatgg aaatggtctc tgacaaggga attcaataca acaacgaaaa
    15781 aaccctgcac atgcttcata tcgatgaggg cgaaaaccct gtctctatcc aactttttgg
    15841 tagcgatgaa gacagcctag cacgcgcagc agaattcatc caagaaaaca ccaagaccga
    15901 tatcgtcgat atcaacatgg gctgccctgt caacaaaatc gtgaagaacg aagctggtgc
    15961 tatgtggctc aaggatccag acaagattta ctccatcatc aacaaggtcc aatctgtcct
    16021 tgatattcca cttactgtca aaatgcgtac cggctgggcg aatccatctc ttgcagtaga
    16081 aaatgccctc gctgctgaag cagcaggtgt ttctgccctt gccatgcatg gccgtactcg
    16141 cgaacaaatg tatactggcc acgcagacct tgagaccctt tacaaggttg cccaagctct
    16201 aaccaagatt ccattcatcg ccaacggtga tatccgtact gtccaagaag ccaagcaacg
    16261 catcgaagaa gttggtgctg acgcagtcat gattggccga gctgccatgg gaaatcctta
    16321 cctcttcaac caaatcaacc attactttga aacaggagaa atcctacctg atttgacctt
    16381 tgaagacaag atgaagatcg cctacgaaca cttgaaacga ttgattaacc tcaaaggaga
    16441 aaacgtcgca gttcgtgaat tccgcggtct cgctcctcac tacctccgtg gaacatctgg
    16501 cgctgccaaa ctccgtggag ccatttccca agctagcacc ctagcagaga ttgaaggcct
    16561 cctgcaattg gataaaactt aaatacgaca ataaaaaccc gtaactcatg aagtttacgg
    16621 gttttattga ttgttgacac tggaagccta ttgttgaatc aaggaaacgc tagagacagc
    16681 tccatctgca cctgttgtaa tctgaatcac tttcccatca cctactttga cattatactt
    16741 accatcatca agtgtgtaag aataaccttt gatagagtag ttttctttct tcccatcaac
    16801 agtttctaca gtgaactggc catctgatga aaccgtgacg gtttcgccgt tagctccctt
    16861 ccagttacct gctgtagctg aaaaatctcc atctaccatg gttaaaacac tcttgtagcg
    16921 tttgttgtgt tccgcttgct tatcctgaag cttgcggtca gttgttgggt cataggttga
    16981 ctggttagac tgggcttgag aatgttgttg agttgaagga gtttgttgac tggaatcttc
    17041 ttgtccttgt cttgattctg gtgccgaaga tgtttgtgtt gcttgtgatt gattagtagc
    17101 catctctgac tgatcagaag atactttcga cttttctgat gagattgaac ttgaggactt
    17161 ctgaatttta ttttccttgc tcgaagaagc tactttagaa ctagaagatt gacttgtttc
    17221 agtagcttct tttttatttc cacaagctcc caaaagcaag gtggaaacca atacaatcgc
    17281 taccattaat ttgatatagg ttttcttttt catttttcta atcatttgta atattttaga
    17341 aatattaggt aatattattg taatattttg cgcaaaaagt ggcaattatt ttctaaattc
    17401 gtttaaaatt tttatttttt ccccacattt agtcctccca ctattctatt cgtaatagaa
    17461 agaggctggg acaaaatagt tctcgaccac aaaaaagcta gagattccca attgcggaac
    17521 tctagctttt taattttgag tcgttctctt gttgtatttt aggaggttat tggccataag
    17581 taccaatccc atgtcaattc tcacttgacg cttccctctc agatgacatc tcttgtaacc
    17641 caaagaagcc tttatctgcc caaagacagg ttccacatca atcttgcgtt gagcgaaaat
    17701 ctgtctacct tggggagata aaagcgcttg acattcttta gctttcaagt tttgatagcc
    17761 ttcgttcata tacagtccct tttgaggggc tgattcaggt tcgtcggcgt agtaaacctt
    17821 gatttcttgt tgaaagtctg tctgtgtttt ctgatgtttg gtatggtgaa aacgataata
    17881 ccagccatca ggatgtgtat agctatcctc cttgtcatta tagtgccaat tcgctaagtt
    17941 tctagctgac tgtttatagc ctctcttttg ttccttatca aacatggcat atttaatcag
    18001 atggtttacc tccttttcat ctaaacgaag gaggttctct tcacttccat atccagcatc
    18061 tgcgacaact gtctttacat catgcggata ggtttcaagg aatggcagaa gagtcttggt
    18121 atctgtcgga tttgaaaaga catcatagtg aagaacaaat tggttttccg tagcgatttg
    18181 aagattataa gcagccttga gttgaccatt tttcatatgg tcttctttca tccgcataaa
    18241 agtggcatct ggatcggttt tggaaaaact gttacgccct tgaaatgtct cctgatagtt
    18301 ctcatatttt tcagcacgta ttgaaaaatc ctccttgact ttacgcaaga ctttcttgag
    18361 tttccgacgt tgggttttac gctcatcctt ccctttaata gctgtctcct caatgtcctg
    18421 gttcaggttt tccaattctt cttcaaggac ttgagcgaat gcaagcaact gctctgaggg
    18481 gataggctct tcttcctcca acttaatggc ctgatggata aggggagtga tttcttcttg
    18541 aaaatagact tgtatctgtt cttgaagttt ggcggcaaac ttgtccgttg ctttcttcca
    18601 cacgaaacta tacttattgg cattggcttc aatcttagtc ccgtcaataa acaagcaatc
    18661 taaggtcacg aactcttcca tttttaaacg aagattgagg tcgatgaaaa gattgcgaat
    18721 gagttcttcc atcccttcag ctacacgaaa acgattgatg gtgcgatagc tgacaaccaa
    18781 ctgccctgtt aggtattgca tagccagatt tttaatcatc attttttcaa tttttcgtcc
    18841 agagaaaatc ccttgtgaat aggcaaatag aagagcagat acaagcattt tagggtgata
    18901 agacgggcga ccaaaggcat gatagaaggt gtggaagtaa ggtattcacc actttttcaa
    18961 tagtaaagac gagatggtct tgcggcaaga aggaactgat ttctagtggt aaagttgttt
    19021 gatttatgtt atagtgaata tgcatgggat agcctttcta aatggtttat tgggcaattc
    19081 tattttacca agactatcgc aaccatgcaa aaaaaagcaa cggcaaatcc gttgcttttt
    19141 ttggagataa gagacttttg tcccaggctc tttaattttg tcatctgtaa ctacatatag
    19201 ggtcttcgtg aagcttattt agttgactgg tattcactaa agtctttatt tacacgatta
    19261 acaaaatcat ctaaagagga ttgtgctact tgctacttca atcagtgtac gcgagccatc
    19321 agtcaattcc tctttagcat tacgaatttc ttcatctagt tgtgcatagg aatcatagtt
    19381 atttttagcc atttcagccg cagcttgata agcctctaaa acaacttttt caaaatgcaa
    19441 acggaatggg ttcttacgca tcgtttgatg agtagataca cgagcattgg tttcgtctgc
    19501 ttggacagta tttgccattc ctacaagtac caacgttgat acagctaatg ttaatgttaa
    19561 tttgattata cgtttcatat caaaacttct atcttataaa aattgggtta aaacggatag
    19621 ataccatgta taatagatat ccttactaga ttggggttct gcccaatcta gcatcaaaca
    19681 tctaatacag attcttttga aaaatgtagt cattattttc ttcttttcat cgcgattaat
    19741 gaagttccag ccaaaccaag gagaccaatg atttcaagaa ctaaattagt tgctgcccct
    19801 gtggatggca aattcttctc agttgctgat gtaggatttt cttttgccat tgtttcattt
    19861 ccagtagcca gtggtttatg attaacttct ttattctgga attttccagt ttggttttct
    19921 cctatctgtg tttgaccatg ttggacagaa ggtttaatct gcttaggtgt ggtgacattt
    19981 tgattcttag aaagtgtctt aatcgcaaac aaactgaagt ggttggtttt aaagacaact
    20041 tgcccatttt caactttaga aggaatacgc tcaaggtcac cattttcctt tacgtgatag
    20101 acgtgaacat ctgatgcagt ctgcccaagc gccagcctaa cagttcgttc tccattgaca
    20161 tgagtttcct tgcctccttt agataaagaa agatcaaaga tgcgaacagt tcctccacca
    20221 gtttggcgag cgattttttc agccaactcc ttcgtcgtca cttcctttat atttacctta
    20281 tcagcatcaa cagccttatc aaatacaacc gtaaccttag ttttcccgtc agaagctgta
    20341 atgatttttg aagtttttgg ggtttctggt tgtggtttaa cctctggttt tggtttttct
    20401 ggttgtggaa tcggcttttc ctgtgttgga acagacggtt ttttgttctc cggttctttc
    20461 ggtgtacttg gttgcttacc gtctggctgt gggattggtt taggttccgg acttgtactc
    20521 ggaacttctg gctgaggttg aaggtctggt ttggtatccg gggctggcat tgaaggtttt
    20581 tgatcacctg cttgcgctct tccactgtct gatttaattt tttctagggt tttaaccttg
    20641 ctcttagctg tttctacctt ggctttagca gtattaattt tgtcagtatc ttgaggtgtt
    20701 tgggcttgag cttgtgcaag ctcgagctct gcctttgcga cttctacttg tgcttccgca
    20761 atttccagtt cgattgtttt actagtattg gttgggtagt tacggcgatc ttcttctttt
    20821 tgagccttgg cttttttctc agcctcttca accttcttct gagcttctgc taccttgtcc
    20881 tgtttcttga gctctccctg acttgatttc ttttctagtt cctcgataat actgtggatt
    20941 ttgttaaaga ccggtaaaaa gtctctttca aaatacgttt tcaaggcttc cccctctaag
    21001 gtagcataag tactgttagc cgtttgtttt agcttttcag aaaatagtct ttcaacttca
    21061 gccacattag gatgtttttt tctatatgtg gctatctttc tagtcatcgt ctcaatttgt
    21121 gcatgaaatt gataatagat aaattcagca acagcatctt ttaaagtttt ttctgtatag
    21181 gtctgttttt caagctgttc tttgaatacc tggattttct taggcgcagt atccttacta
    21241 ttatcccaaa tatctttttt ctgcactttg taatctggat cattatccgg tagctctgga
    21301 tagttattga cttctctgag atagtcctct agctttttca attcttcttg gccttgacga
    21361 atcaagtctt gtaacttttt cttttcagct tcggtcgcct tttgtcctgg acttggggaa
    21421 gttgatggag gtgtttgcgt caccggtggt acaaccattg aaccgcctgc ttgtcctcct
    21481 ggttggggta cgactccagg tccttgtcct actgaagggt cacccccccc ctgtaaacca
    21541 tcatttagag agagctcttt aggagtactt ttactacctg aacgattttg aaataacttt
    21601 atttcttcat ctactttttt cttaacttcc cttcccagct cctctaccat gcttttagta
    21661 gaaactctat caatcttatc tctgtattta tctacaacca cctgaacctt tgtgacaatt
    21721 tctgaaaatc cagaaacaga tcgatccagt gagtctaaac gactagtcac atagtccgaa
    21781 atttctttgt taactctttg tacagcatcc tcatattctt ttttatcacc tggagatact
    21841 atttgtggaa tagggggatt agcagaaaca ttctctgtcg catgaaccac acttcccata
    21901 aaaagactgg caacagctac actagccact cctacactaa atttgcgaat agaataacgc
    21961 attcttcttt catgatttga tttagacata tctatatcct ccataatttt aactaactac
    22021 agtatatcct gctaatatga attgtatatg aaatttgcta cattattttc gctatcctct
    22081 cattcttcca tttggaataa taacttcctg cagtaatttc tcttcctctg ctattctatg
    22141 gtataaaata gtagcatata taggaaaaat aaagatgaat gtcacgtaag catggctcac
    22201 tagggctaaa ccaactagtt caggtaatat atttaaaaag taattaggat gctttacagt
    22261 tctaaaaagg aagtgatcta caaatttgtg attattgaca agcataagct ttactgtcca
    22321 aatatctccc aacaagtgaa tcaccaacat caacatcagc atagaaaaaa taagcaaaac
    22381 taaaccaacc atgctcatgc catcaaaaat tgtcttgtgc accatcgcct caacaaagca
    22441 agagagatag aaaataatat gtgcaaacgt caatcgtttt gtattttcaa ccccgaattc
    22501 ttgcccacca ttgctaagga tccgtttctc attctctatt gaacgtttta aaaaaatcaa
    22561 tcgaataagg aatgttacta aaataattgc taataccata ttagtatcct ttcttttatc
    22621 tatacaaagg ataacacccc tatgttaacc ctatatgaac tgtaacaaaa aagaacggaa
    22681 tactttccgt tcctgctatt tgagcaccta atctatctgc aagtcaatct taaactccat
    22741 accgcgttca taggggagaa aactataatc catttgataa ctttctaaaa tattattcac
    22801 aatgtaaaga cccaaaccgg aaccttcagc cttatctgtt acgatttgag aatgatagaa
    22861 tatatcaaat aaatgctcta gttcttggtc acttagaggc gcacatgtat ttttgattga
    22921 gagatagcca tcttgctcag atatcatcac acagccattt tgatctgaat atttgatagc
    22981 attactaatc aggtttgtta aaaccttatc taatgctttc agactcatgc taactcttgt
    23041 agcatctgtc aattgatttt caattgtaac gcctcttgaa tgagctaatt cttgataacg
    23101 tgataaaata tctactaaaa ctgttttcac agtcaaggtc tcacgacact ctgtccattc
    23161 ttgaaattta gaagactcca atacttcttc taacaggtgg ctcatctggt caatcgtatt
    23221 tatactcctt gctatatact taggatgatc tttataatcc ccaatattgt actgcatatt
    23281 ttctagaata attcgaagac tggctaaagg ggttttcaac tcatgtgatg ctccacgaac
    23341 aaaagaaact ttctgatttt gcaacctgac aatccgttca tttcttctct ctgattcatc
    23401 aatgactctc agtaagtttt catacacatc attgatttgc tttccaactt ccccaacttc
    23461 atcctttcgc atttcattaa agcgaatatt atgatccaac tcctgcatct tacttgtaat
    23521 ttttgatata taaaagagag gctccaataa acgtttcgta tagaagtagg aaaagagaaa
    23581 ggcaaataca agcgttgcta gaaaggtata agggagatag agaaacataa ttcgtgttgc
    23641 ctctttataa atatccacac cttgaataaa ttgaatggtt acctttttac catcagccgt
    23701 cgtaatctga cgatcaattg ttacgatata gctagtggga cttccttcct ttatttccaa
    23761 gtcgtgcaca agggaaattc tcttatccag tacatcatct ttcaaatggg cagatctgcg
    23821 aagagacttc gaataggatt ccagcaaact aggcagttcc tgaatctttt tcccttccaa
    23881 atatttgacc ataatagtcg cactctcatt aaacttctca ccttcttgct gaatgtaata
    23941 aaaaggaaag gtcagataga tagcaatgtg gataatcaaa gcaattgcac caaaaagcga
    24001 aaaacttctt aaaaatgatt tggttaataa tttaggattt tttatcataa gctaatttta
    24061 tatccgacat ttttcactgt tacaatgcaa tccagatcca acttcttacg aagatttcga
    24121 atgtaaacat caactactct atcaagaggc aaatctgcaa aatcatttga gatagactcg
    24181 agtatctgct ttctactcaa aatctggttc ttatgttgaa tcaaacattt taaaaccaac
    24241 aattctttcg gcttaatcgc aacctcctcg ttcttaactc gagcttggta ggatgtgaaa
    24301 tccactgtca catcctgata atgccaaata tcctccacaa catagtaacg cctaagcaag
    24361 gcttgaatac gcttgactag gactaccagt gaaaatggtt tcgtcacatg gtcatcagca
    24421 taaaggtcaa atacctgcat ctgagtttcc tcatctccga gcgcacttaa aacaatcaca
    24481 ggaatatcat gatttctatt aatttctttt aacacttcaa aaccactctt cctaggcatc
    24541 attaaatcca gtaccatgag atggataggg agatcttgaa atttttccaa ggcctcctgt
    24601 ccatccttag ccacaatgac atgataaccc tcttctgtca gaaatgctgc aattccttct
    24661 ctaatcattt cctcgtcatc tgcaactaaa atgttcatcc attccccttc ttaacctatc
    24721 taagattcac tcttttgcta ggtttacatt atttccattt cgtacgtaaa aaagaaacag
    24781 ggctctattg aaatggtgga aatttcagga aactctgctc tttttcattc tatccaaatc
    24841 ttgacatcta tctattctta ttataaccaa catttacata ttgtcaactt gtttcctttg
    24901 gttttataaa aataaatata aaaaaatcag gattgctcct gattctccat ttttatgcaa
    24961 tatcaaattt taactggtct gctttgacac caatcttgag agtcttacct gctaccagtt
    25021 ctcccttgag gaggagttct gccaacttgt cctccacttc tgtttgcagg gttctgcgaa
    25081 gtgggcgagc tcccatctct gggtcatatc cttgatttgc taacaatttc agagctgaag
    25141 cttgtaattt caagtcaatg cctttttcag ccaaacttgc cactaaaggc ttgaccataa
    25201 tcttcaccac ttcctgcata tggtcactag ataggctatg gaagaccacc ttctcatcaa
    25261 tacggttgat gaattccggt ctataagctt ttttcagttc ttcaaacatg cgtttttcca
    25321 tattttcctg gtcaaaacga atatccttag ccccaaaacc aacagtctta tcatcacgaa
    25381 gggcagtcgc acctagattc gatgtcataa tgataatggt atttgaaaaa tcgaccttgc
    25441 gtcccttgct atctgtcaag acaccgtcat ccagaacctg caagagaaca ttaaagatat
    25501 ctgggtgggc cttctctacc tcatcaaaga ggagaacgga atagggttta ttgcgaacct
    25561 tctctgtcaa ctccccacct tcttcatatc ctacatagcc tggaggagct ccgttgagac
    25621 gactagctgc aaatttctcc atatactcac tcatatcaaa gcggataagg gctgattcgt
    25681 cgtcaaaaag aacttctgcc agagccttgg ctaattcagt tttcccgaca cctgtgggtc
    25741 ctaggaacat aaaggaaccg atcggacgct tgtgactgcg aattcctgac tgattacggc
    25801 gaatggcacg actaatactt gaaacagctt gatcttgacc gataacccgt ttatggagtt
    25861 ctgcttcaag atttagatat ttcttagcgt cagtctgagt cagtttttga actgggattc
    25921 ctgacaagcg actcaaggtg gtcaaaatat cagactctgt caccaagtct ttgtagacag
    25981 gtacttcctc ttcttttgcg attagctggg ctgcctgttt ccacttgcca tccatcaggg
    26041 ccttgtcagc tggactcaaa tctgaatcgt ctgcttttac atgctttgcc ttattttgca
    26101 ctgttgctgc tgcttcgtcc aagaggtcga tagcagagtc tggcaagtga cgactggtta
    26161 aataacgatg agccatctta accgctgttt caaccgcttc atctgtgatt tgtacacggt
    26221 gatgtttctc ataagtcgcc ttcaaacctt gtaaaatagt catactatct gccacacttg
    26281 gttcttcaat cgtcacttta gcgaaacgac gagaaagtgc cgcatctttt tcgatatgtt
    26341 tttgatattc ttcctgagtg gtggcaccaa ccgttctcaa agttccacgc gccaaggctg
    26401 gtttcaagat attggccgca tccagagtcg aatcaatccc gctaccagaa cccatgatgg
    26461 tgtggagttc atcgataaag aggatgactt ggccatcttc ttcaatatcc ttgatgatat
    26521 tattcatgcg ttcttcaaag tcaccacgga agcgtgtccc tgcaacgaca ttcatcaaat
    26581 caagttctaa cacgcgcatc ttagccattt ccgcaggcac gtcaccacta gcaatacgct
    26641 gggcaagacc aagggccaga gctgttttcc cgacaccagc atccccaacc aagacagggt
    26701 tgttcttagt cttacggctc aagatttgaa tcatacgtga gatttccttg tcccgaccga
    26761 tgactggttc taacttgcca gaatgcgctt gctctgtcaa atcatgcgta tagtcctcaa
    26821 gaccaccact aggagtctgc ggcatgccca tcatattggc catagaattt tgcttgtcag
    26881 ctactgtacg atggcgttgg cgtaaagcct tgagatcttc acgagtccag cctgcccgtt
    26941 cttctaaatt tcgacgaaga gcagcaatct tgacctgatc tttcttgtct tcataagaaa
    27001 aaccagccct ctccaagata cgagtcgcca aggcattgcc atcatgcaaa atcgcataga
    27061 ggacgtgctc tgtccctagt accttagcat ggaccactga cgctacatac tctgcttcat
    27121 caaaaagaac ctgcaaacga cgggagaacg gcaattccgt aaaggtttca tcctggctat
    27181 agtccgtttc agtcagttcc aaagccacct cttctaaacg gtccatctca tacggataat
    27241 catttaaagt tgcccctgct acactataac tgtgattaga catggcaatc aacaagtgcc
    27301 acgactctag ataacgagct ccaaaatgtc cagcaaccat gtaggcactt tcgatacatt
    27361 cattcaatgc ttttgaatag ttcatcttac ttcccttttc tatctacctc ttgtatgatc
    27421 tgacgtagca tgtttgctcg aacaactgga gcttcttctc ctaaaacgcg atccaaagct
    27481 actgattcta gcaaattcat ctcctgcttg gtcatcaatt cctgctcaac caaaagctgg
    27541 agaatatcct cataaatttc ttgactgact cgctcaccaa tcgagtaaag cagctcccgg
    27601 agcatttcat gatgactaga aaactcaatc cgtcctatac gaatgtagcc tccgccacca
    27661 cgcttacttt caaccaagta gcctctactt tccgtaaagc gtgtcttgat cacgtagtta
    27721 atctgactag gaacaacctg aaaggtatct gccaactgac tccgttgcaa ctccacgata
    27781 ccagattgat ctaaaatcgc cttgatgtag gcctcaatat gatccgatgt atttttaaat
    27841 ctcataacaa acccacctct ctctttaaac cttgaccttg tttgactatt ataccgaaat
    27901 tttctcattt ttaaaagaaa aagggcgctg gtaaaggata atcttcacca actccctatt
    27961 tttctactta tctaagccta attctgccaa gatttgacgt ttgtaggcaa tcttttgaac
    28021 ttccttgtcc tcatcttcag accaatctag tttaatttct gaaacaatct gcccagggcg
    28081 atttttcaag atatagatgc ggtcgctgag attgagggcc tcctcaatac tatgcgtgat
    28141 gatcagggtt gttagctgca actgcttgtg aatctcaaga taccaagcgt ggagttccat
    28201 ctttgtcatc tcatccaagg cgctaaaggc ctcatctaag agaaagagct tgtgcccaaa
    28261 aaggtaggtc cggagtaagg ctacacgctg gcgcatccca ccgctaagtt catgaggata
    28321 cttgtctctt acagctgtca gctggaaggt cgcaagaatt ttatccgctc gggaaatagc
    28381 ttctgcctta tccacctttt gaatcaagag gggcagaatg atatttccaa gcaccgtctt
    28441 gtgctccaag agcagatcct tttgcaacat ataactcacg cgccccttga gattttcttc
    28501 accatcaagg acaattctcc ctgactgaac ttctaaaatc ccagcgatta gattaaagag
    28561 agtggtcttt ccaacaccac ttgggcctag gatggaaacc acttcgcctg aagtcacctg
    28621 taggttgata tcctctaaaa tcctctcctg accataggca taactgacgt gctctagtct
    28681 aatttctgtc attatttcac aaattcgttg gtgaagcctt tgtctgtcaa gtcttcttta
    28741 aggataccat tttctttatc ccatttgtag aaagcattcc agcgagctgc gtcaaattga
    28801 ccccattttt ccttgtcgct tgcgtattct tttgacaagt atttttgaga ttcgatgaca
    28861 aagtcacgtt tttccttgag ttcaggtgca ttcttgatga gaatatctgc agcttcttct
    28921 ggatgttcca tggcatattg gtaacctttt ttgatggctt ggatgacttt acgagcttct
    28981 tctttgttgt ctttgaggta gtcgttgttt gcgatgataa ctggtgagta gtaatcaaat
    29041 tccttgacat agtctttcaa atacatgaag ttagcatcta caccttgaga tttagcaagg
    29101 ataccatccc aaccgtagta aatccaagca gtatcaaaga cgccattggc aatcggtgtg
    29161 attgagtttg agtcgttatt tggtactttt tcaaccttct caaagtctcc accttgagat
    29221 tctaccaagg ttttcaacat agcaagttca gttgggtcat tccatgtccc atatttttta
    29281 ccaaccaagt cttttggact gcttacatta tcagatttac gagagatgat tcctgatgta
    29341 ttgtgttcaa caatagctgc aacggcagtg attcctgctc ctttttccaa tttcttagcc
    29401 atgtagtctt ggaaatatac tgcaaatggt gcctttccgt tgataaccaa gtcagaagaa
    29461 ctttcttctg gtggcaattt caaatcaaca tccactccag cttctttgaa ataacctttt
    29521 tccttggcaa catatagccc tgtgtggtta gtgtttggtg tccagtctag gataaagtcg
    29581 atcttcttga gttctgcttc tttgttgtcc ttagaagcag ttccttgacc acaagctaca
    29641 agcacgacag ctacaagagc tgttaaaaca gttaaaaaca ctttccatgt tttcttcatt
    29701 ttgatttcct cttttctttt tcagaaacat tctaattcta cgaacgtttc catttaatca
    29761 catatttttc actgatatcg accagcttca tacccaaaag actgataatc gacaccagaa
    29821 taataatggc aaacatggta tcatactgaa acagtttttt agactgaatc atataaacac
    29881 caagaccttc aaaacctccc aaccactcag ataccacagt tgtgataaag gcgtaggaga
    29941 cactgaccct cagacctgca taaaagtaag gcaggctaac tgggatttta aaatgccaca
    30001 ggatttgcca aggcttggcc cgcatcagac taaacaaggt cagcatatcc ttgtcgcaat
    30061 gcctaaaacc gtccaaaata ctaacgatga tgggaaaggt tgtcgttaag ataatcaaga
    30121 caatcttggg caaaatccca taacctagcc acaagaccag gataggagct atggcaatgg
    30181 tcggaatggt ctgaatgacc accatcatag ggtaaatcag gtcattgagc caagtcaaac
    30241 tatccatgag cacagccata agacaggcaa tcaaaactcc caaaatcagc cccagtaaag
    30301 ccactctcaa ggtcgcccag ctatggtgcc agagaaattc tctgtcacga acaaagggct
    30361 ggagaatttc aagaggtgtc ggcaggataa acttggggag aagtttaaga aaacctgcta
    30421 actgccagat tgacaagact ccgagaaagc ccaatagact aatgtgtcgt ctcagtatac
    30481 ttttcaagtt tctcatcaat acttaaaatc tctcctacat ttattttgac attggcaaag
    30541 acattgtctg cctcctgccc tgccacttcc agcgcttctt ttagaatgcg cataagctca
    30601 tcaaactccc cttccaagac cgtttcaaat ggtgtcacta ccatcgtcac ttcttgagtt
    30661 tgcagataag caatgacctg atcaataaca gctatccgat caatcccctg tgctaggggt
    30721 aaaacttgca aggcaatgct tgctttcata agaacctcct cttctcgttt tccttttaaa
    30781 aaaaagaaaa acctttacca tacatggaaa aggtgcaaaa ttagactaag tatcgttccc
    30841 tccgctggca ttacccagat caggtgcggt cgaagtttaa cacttcctct cagactgtac
    30901 acagactccc atatattata tgtatatatg atagcgcaaa ataagaaaaa tgtcaaggaa
    30961 actgcttaca gaaaagagca ggaaatttct cctactccaa ttttctatac tcgttctcca
    31021 tcactgttta ctctgtagcc atccaccgtc gtattaacag ctaaggcgcc tgaagagtaa
    31081 gaataatacc acttaccaga gacctggtac cagcctgttt tcattgcccc tgaactaccc
    31141 agataatacc acgagccatt ctcctttaac catcctgtct gcatttcacc agataatttc
    31201 aaataatacc agctagaacc gtctttcagc catcctgttg atttagaacc atttgatttg
    31261 aaatgatacc aactaccatt tattttcttc caaccgacag ttgaactatt attggatcca
    31321 tagctagaga tatttcctga ttgattctga taattttgtg aagaagtcac tccctctagc
    31381 tgtatatagc gacgacttcc gttataagcc gtataactca accacttgta gccgtctttt
    31441 tcaagtatct gatcataatg aaccttctcc ccaggataat agtaatcaat cacagttgca
    31501 ctaactaggg gttcagtttt gatagcagac ttggtcttaa aataatgagt tccacctgtt
    31561 gaagaaagaa cagaattacc tagaggattt ttattcacag cctccaattg aacataacga
    31621 tagcttccat tataggcagt ataactcaac cacttgtagc cgtctttttc gagaatttga
    31681 tcataatgaa ccttctctcc aggataatag taatcaatca cagttgcact aactaggggt
    31741 tcagttttaa tagcagactt ggtcttaaaa taatgagttc cgcctgttga agttgaggat
    31801 tgactattcc caacactgcc accatccaaa tctttaaaat gaataaatcc tgtcatcgtg
    31861 tttgctttta taattcgttt attataggat tctgtataac catagttata ttcctcaatc
    31921 tcaatctgat ctcccattac atttgacacc caggcaacat gaccataagt tcctgcagta
    31981 gaccaagtaa tggaaccaat cgtcggtgta ttatctacac gataaccttc ccgacgagca
    32041 cgatgtcccc attcattcgc atttccataa gctgccggaa tttcaaaacc attgacatta
    32101 ctcaaacgaa aggctacaaa agaagtacat tgacgagaat acatgcgcca ctgatcaatc
    32161 tcctggctcc catttttata ataagcagga taatcatccc cacgcgcaat cgatccattt
    32221 cctctggaat aggcatagac actatcacca gctgctagca tcaatcctac tactaaaaaa
    32281 ggaacaagct ttttaactga catcttcaag taataacttg ttcctcttgc tataaacggt
    32341 aaaattttca ttcttcctcc ttaaaaaata atataaagcg atgattgcct tcacctaaat
    32401 tattcggaga aaaaagaaaa agtgagaaaa tttctcactt tagtcaagat aatgaatcaa
    32461 attcttttct gtaagaatat ctgagtaagt aaaggattca acgtaaacat taacttgttt
    32521 atctccttcc acatccccaa actccacaat aaatagggat ggataaactt caattagctt
    32581 acccaatcta tttttttggc gcttacgacc attctccaaa gtcatttcta cgacttgtcc
    32641 ctcatgtgcc ttaatttctt ctttgatttt tttcatcttg gctacatctg taaatgcatc
    32701 tgacatctta tgcctccctc tttgagatac ttgaaaattt attgtattct ttttggaaaa
    32761 tcaattccac tgttccacga gctccactac ggtttttctc gataataact tccaccttat
    32821 tatttgggat accctcctct tcttcaccac cacgttcata gtagtcatcg cgatagagaa
    32881 aagctacgat atcagcgtcc tgctcaatag acccagattc acgaatatca gacaagaccg
    32941 gtctcttgtc ctgacgttgt tctacaccac gagaaagctg actcagagcg attactggaa
    33001 ccttcaattc cttggctagt attttcaact gacgagaaat ttcagaaact tcttgttgac
    33061 gattttctcg accagttccc gtgataagtt gcaaatagtc tatcaaaatc aaaccaagat
    33121 ttccagtttc ttgagccaat ttacgagaac gagaacgaat ctctgtaatc cgaatacctg
    33181 gcgtatcatc gatatagata ctggcgttag ctagattacc ctgagcaata gtatattttt
    33241 gccactcctc atctgtcaat tgccctgtac ggatagaatg tgactccacc aagccttctg
    33301 cagctaacat acggtctacc aagctttccg cacccatttc gagtgaaaaa atagcaaccg
    33361 ttttgtccaa cttagtccca atattctgag cgatattcaa ggcaaatgct gtcttaccaa
    33421 ctgctggacg agctgctaag ataatcaact cctcctcatg aagtcctgtt gtcatatgat
    33481 ccaaatcacg ataacctgtc gcaatacctg taatatcggt cgtttgttgc gagcgagctt
    33541 ccagatttcc aaagttgaga ttcaacacat ctcgaatgtt cttaaacccg cttcgatttg
    33601 cattttcact gacatcaatc agcccttttt ctgcctgagc aataatttca tcagctggtt
    33661 gtgacgcttc gtaagcttgg ttgacagact ctgtcaactt ggcaattaaa cgacgtagca
    33721 ttgctttttc tgcaacaatc ttagcataat actccgcatt agcagaagtt ggcacagaat
    33781 tgacaatctc aaccaagtaa gacaagccac caatattctg taaatcacct tgattatcaa
    33841 ggatagtacg aaccgttgtt gcatctatgg catcaccacg atcggataaa tcgaccatgg
    33901 cttggaaaat caaacgatgg gcatacttaa aaaagtcccg agactcaatg tattctcgca
    33961 caaaaacaag tttactctca tcaataaaga tagcccctaa aacggattgc tcagctaaga
    34021 tatcttgagg ttgtactcgt aactcttcta cttctgccat cagacttctc ttccttttac
    34081 aatcttgtca agaaggtgta aacttatcct tctttcacac gaagattgat tacacttgtg
    34141 atatcttgat agattttcac tggcacatca atcaaaccaa ccgctcgaat cggagcttgt
    34201 acttgaatat gacgtttatc aatcttaatt ccaaattgct tttgcaattc ttctgcaatc
    34261 ttcttattgg taatagaacc aaaggtacga ccatctggac caactttttc aacaaattct
    34321 acaacagttt cttctgcttc aagttgtgct ttaattgctt ttccttctgc aatcatctca
    34381 gcgtgagctt tttcttccga tttttgttta ccacgaagtt cacctacagc ttgagcagtc
    34441 gcttctttgg ctagattctt tttgataaga aagttttgcg cataccctgt tggtacttcc
    34501 ttaatttcgc cttttttacc ttttccttta acatctgcta aaaagattac tttcattctt
    34561 ctttctcctt ttcctttatt tcatttaata caatttctgt cagtttttca cctgcttctg
    34621 acaaggttac atctttaatt tgagctgctg ccaaattaaa gtggcctcca ccgcctaact
    34681 cttccataat ccgttgtaca ttcagtttac tacgacttcg agctgagata gagataaatc
    34741 cttgtgtatt cttcgcaaga acaaaactcg cttcaatacc tgacatggct aacatggcat
    34801 ctgctgcctt actaataaca actgtatcat agcatttcgt gtccttagcc tctgctatta
    34861 gtacatctga acctaattta cgcccctgta aaataagttc attgacctca cgatattctt
    34921 caaaatctgt cgcagcgatt tcctggatag caatactatc acttccgcgc gttctgagat
    34981 agctagcaac atcaaatgtc cgactagtta ctcgcgaggt gaaattttta gtatccaaca
    35041 tcataccagc catcaagaca cttgcttgca tacgactcaa acgatttttc ttagaattct
    35101 ggaactgaat caattccgtt accaactcac tggcactact tgcaccactt tcgatataag
    35161 taataaccgc attatctgga aaatcctgat cccttctatg gtggtcaata acaatggttt
    35221 gggtaaataa atcataaaat tcttttgata atgttaaggc tgtctttgaa tggtctacaa
    35281 gaatcaacaa agaacgattg gtcaccatcc ccattgcatc cttaacagac aacaacttcg
    35341 taactccttc tttttctatg aatgaaacag ctcgttcaat atctggagac atttgttctt
    35401 catcataaag agcatagcta ttttcaatca cattgctggc gaacaactgc atacctacag
    35461 cagagcccaa agcatccatg tctaaatttt tgtgaccgac tacaaaaaca tgatctacac
    35521 tccgaatctt atctgaaata gctgtcatca tagcgcgcgt acgagtccgt gtacgcttga
    35581 ttgaagcagc agacccacca ccaaaataaa ctggattttt cgtttcgtcg ttttccttaa
    35641 caaccacctg gtcgccacca cgtacttcag ccaagttcaa attgagcaaa gcaactttcc
    35701 ctatctcatc atgatttcca tcgccataag aaaatcccat acttaaggtc aagggcaact
    35761 gtctctgttt cgactcttct ctgaaagcat caataacaga aaatttatca ttcatcaagc
    35821 cctcaagcac cgtgtagtca gtaaatagat aaaatcgatc catacttacc cgacgagaaa
    35881 acatcatgta tttttctgaa aactctgata taaaattagc tacaaaacta ttgatttgac
    35941 taatatctga ctcagaagtt gcatcctcca aatcatcata attatccaca gagacaatcc
    36001 caatcactgg tcgacttgtt accaattcat ctgttatggc ttgttccctg gatacatcta
    36061 caaaatacaa aacaccggaa gaagcatcca tatgaacagc ataacgcttc tcaccaagct
    36121 tggcataagt agacggattt cctactgaag ccttgataat cgtttgaaca gcttctaaat
    36181 caaaatcacc atcttccttg gtcaaaatca attcagcata gggattaaac cactcaacct
    36241 ctccagaaga taaattcaat ttcataacac ctacaggcat ttgttccaat agagatgtca
    36301 aactttcttc cgcttggtgg tttacatact gtatctgttc tacatcactc cttgtataat
    36361 gcactctcag tttcttaaat aaaaaaacat agcctcctac aaaaagaaac aaaattaaaa
    36421 ccgtcaacag attattatta acaaaaataa tgaaagtgga taagactcca aacgcaatca
    36481 atcctactag aataggaaaa attggactta cataaaattt tttcattcaa aacctcttgg
    36541 cacccattat accataatac ccctcaaaaa gcgacttttt aaaagtgtaa tcagtaattc
    36601 tatcaattat aagaaaaagg tagtttacaa ttcagtaaac ctacctttac acatattgaa
    36661 attaagattc tttaacctct aacaaaccaa tttcgccatc ctcacgacga taaatcacat
    36721 tggttgtctg atcttcaaca tccacataga taaagaaatc atgccccaat aaatccattt
    36781 gtagaattgc ttcttccaaa tccattggtt ttaaatcaat ttgttttgaa cgaacaactt
    36841 tagactggac aacatttgaa tcttccacca aagcatctgt aaataattga ccagttgcta
    36901 ccttattttt atttttacgc tcgatttttg ttttattttt acgaatctga cgttcaattt
    36961 tatcagttac aaggtcaatt gaaccataca tatcttgaga tacatcttct gcgcggagag
    37021 taatagatcc aagcggaatc gttacttcca ctttagccgt tttttcacga taaactttta
    37081 agttaattcg ggcatccaac tcttgttctg gttggaagta cttttcgatc ttttcgagtt
    37141 tagaaactac ataatcacga attgcttctg ttacttctag gttttcacca cggatactat
    37201 atttaatcat atgagtacct tctttctaaa catttttgtt tttatgattt tattataacg
    37261 ctttcattct atttttgcaa attttttcct catcttacaa gggaaaatgt ttttacatcc
    37321 ttagcaccag cttcttccaa cagtttctta acacgattta tagttgctcc tgtagtatag
    37381 atatcatcta taagtaggat ttttttagga atagtgactc cacttttaat aaagaaagga
    37441 agttctgtcc ccaagcgctc tgaacgattt ttagaagaac tggctctctc ttctcttttc
    37501 tctaataaat ccagatactc aaagcctgct gcctctacca agccctcaac ctgattaaat
    37561 cctctattag catatctatc aggacttagg ggaattacaa caaattgata ctctttgtac
    37621 tttttcaact cctcacttaa aaatgaagcg aaaacttttc ttaacaggaa gtctccatca
    37681 aacttatacc gactgaaaaa atccttcata gcttgattgt aagtaaaaat cgctctatga
    37741 ctgactccaa ctccttcttt acaccaaaat tgacaatctt gacactttgt tgacaactct
    37801 gttttcatac aatttggaca gttctcttcc ccaattcttt caaaagtaga atcacagtct
    37861 gaacaaagac aagagtcatc attcctcaga agtaagagac tactaaaagt taaaacagtc
    37921 ttcatagtct gcccacataa caagcacttc atagaccagc ctccttattc atcatctgaa
    37981 tttccttaat cgccttcttg attgaagcat ttaacccatc atggaagaaa agcaaatctc
    38041 ctgtcggtct atccatgctt cgtccaactc gtccaccaat ctgaatcaaa ctagacttgg
    38101 taaacaaacg atgattggct tctactacga aaacatccac acaagggaag gtaactccac
    38161 gctccaagat tgtcgtactg ataagtattg tcagttctcc atctcgaaaa gcttgtactt
    38221 gctctaatcg atcctctgtt acagaagata caaagccaat tttctcattt ggaaattgct
    38281 cctgtaagat ttcttctaac tgctcccctt tcttaatttc tgaagcaaaa atgagtaacg
    38341 gataagctgt ctttctctgc ttctcaatat aggactttaa ctttggtgac aaacgattct
    38401 tgtctaagta gcgattaaaa tccgataacc aaattggttt tggaataatc aacggatttc
    38461 catgaaaccg tctcggtaaa ttcagtcttt ttagttctcc taaacggacc tttttatcta
    38521 actcattggt cgaagtcgct gttaaaaaga ttctcaatcc attctccttt acactattct
    38581 tgacagcatg gtaaagcgtg ggattatcaa cataaggaaa agcatctact tcatccacta
    38641 tcagcaaatc aaaagcttga taaaacttca ataactgatg ggttgttgca acaactagtg
    38701 gtgttcgaaa ataaggttcc gattctccat gtagcaaagc tatcccgcaa gaaaaatcct
    38761 gttgcaggcg cttgtacagc tccaaacaaa catctatgcg aggactagcc aaacacactg
    38821 caccacccgc attgatcact ttagccacta cttgataaat catttctgtc tttccagctc
    38881 ctgttaccgc atgaactaag gttggctttt gcttgtctac tgcttgaagc agtccctctg
    38941 acaccttctc ttgaaaagga gttaattggc cgcgccattt gagaacatct tgcttcggaa
    39001 aatcctcctg cggaaaatag tataaagttt gatcacttct gactcgcttc atcagcaagc
    39061 actctcgaca atagtaagca ccgatgggca aataccattc ttctagaata gtactattac
    39121 aacgttgaca gaaaagtttc cccttctcct ttctcattgc tggaagtttc tccgccaact
    39181 gacgttcttc ttctgttaat tcattctcag taaataaacg accgagataa tctaaattta
    39241 ctttcatact tctttattcg taaaaactag cactttagat gattttttag tacaattaaa
    39301 tcatggaatt taggacaatt aaagaggacg gccaagtcca agaagaaatc aaaaaatctc
    39361 gctttatctg ccatgccaag cgtgtttata gcgaagaaga ggctcgtgac ttcattactg
    39421 ccatcaaaaa agaacactac aaagcgacac ataactgctc tgcctttatt attggagaac
    39481 gtagtgaaat taaacgtaca agtgatgatg gtgagcctag tggtactgct ggtgttccca
    39541 tgcttggggt actagaaaat cacaatctca ccaatgtctg tgtggtcgtg acacgctact
    39601 ttggtggtat taaactaggc gctggaggac taattcgtgc ttacgccagc agtgtcgcct
    39661 tagctgtcaa agaaattggt attattgaaa taaaagaaca ggctggcatt gctattcaaa
    39721 tgtcttatgc tcagtaccaa gagtacagta acttccttaa agaacatgat ctcatggagc
    39781 tggatacaaa ctttacagat caagtcgata cgatgattta tgttgataaa gaagaaaaag
    39841 aaactattaa agctgcactt gtggagtttt ttaatggaaa agtcacttta actgaccaag
    39901 gtttacgaga ggttgaagtt cctgtaaact tagtgtaaac aatgaataat acagcgtttc
    39961 gttgacattc tcacaactac tttagcgagc aaaataaaaa gatgcgtacc aaaatatact
    40021 agaaaatgaa gcaattcaaa cgaaacctga tatcgttttc cttcacacct atttactaga
    40081 attaactgaa cgcaatcact tgaaaattaa tgactttgat ctatgatata tagaaatcct
    40141 atcgcttcga taggatttct atattttaca aatggtatgg atagcgttat actaaagata
    40201 tcttatacaa agaggtattc atatgtctat ttataacaac attactgaat taatcggtca
    40261 aacaccgatt gttaaactta acaacatcgt gccagaaggt gctgcagacg tctatataaa
    40321 gcttgaagca tttaatcctg gttcatctgt aaaagaccgt attgccctta gcatgattga
    40381 aaaagctgaa caagatggta ttctgaaact tggttctact attgttgaag caacaagtgg
    40441 aaacaccggt attggacttt catgggtagg tgctgctaaa gggtataaag tcgtcatcgt
    40501 tatgcctgaa actatgagtg tagaacgacg taaaattatc caagcttatg gtgctgaact
    40561 cgtcctaact cctggtagcg agggaatgaa aggtgctatt gctaaggctc aagaaatcgc
    40621 tgctgaacgt gatggtttcc ttcctcttca atttgacaat ccagctaatc cagaagtaca
    40681 cgaaagaaca acaggagctg agatactagc tgctttcggt aaagatggat tagatgcctt
    40741 tgttgctgga gtaggtactg gtggaacgat ttctggtgtt tctcatgcac tcaaatcaga
    40801 aaattctaac attcaagttt ttgcagtaga agcagatgaa tctgctattc tatctggtga
    40861 aaaacctggt cctcacaaaa ttcaaggtat ctcagctgga tttattcctg atacacttga
    40921 tactaaagcc tatgatggta tcgttcgtgt aacatcagat gacgctcttg cactcggacg
    40981 tgaaattggt ggaaaagaag gcttccttgt agggatttcc tcagctgcag ctatctacgg
    41041 agccatcgag gttgccaaaa aattaggtac aggtaaaaaa gtccttgccc tagcaccaga
    41101 taacggtgaa cgttatctct ctacagcact ttatgaattg taaccgtcca ataacgaagt
    41161 ctattgaaaa atctccagac tagagaactc acggatagtt cctaatctgg agatttctta
    41221 tttgcacttt tcttgtacaa ctttagtcca tggtaaatag gcctctaaaa cctctttgtt
    41281 tacgagagtt tccacgtttg gaagacattc tagaagatag gatagatatt tctcactatt
    41341 tataatggat tgaaataaga tatgaacaaa tcgattagga catgatggta aagcgtaatc
    41401 ccttgtttct cagctttccc agacaaaaaa gtccaatagt aagtcagctg actatcactc
    41461 tctagcaccc tataagaagt ttcatccgca tgaagtaagg gctgagtcaa tagtctctct
    41521 cgcaagaggt tataaagggg ctccaaatag tattgactcg tcttgatatg ccgattagag
    41581 atttccttac gtgtgattgg taaacccatc ctagcccaat cttcttcttg gcgataattg
    41641 ggtaccttca gattaaactt ctgatggatg gtgtgagcga taatagaagc tgagccaaag
    41701 ttatgcgcta aaggggcttt aggaatagga gatttcacaa gcttatccag atgattatct
    41761 tttactcgtt atggacaatg ctatatggca taaatcaagt accttaaaga ttccgactaa
    41821 tattggcttt gcatttattc ctccatacac accagagatg aaccccattg aacaagtgtg
    41881 gaaagagatt cgtaaacgtg gatttaagaa taaagccttt caaattttgg aagatgtcat
    41941 gaatcaactc caagatgtta tacaaggact ggagaaggag gtgataaagt ccatcgttaa
    42001 tcggagatgg actagaatgc tttgtgaaaa cagatgagta taaaaagaaa gtcctcattt
    42061 caatagaaat cacgactttc tgatgaattt atagtaaaat gaaataagaa caggacagtc
    42121 aaatcgattt ctaacaatgt tttagaagca gaggtgtact attctagttt caaaccacta
    42181 tatttgggga gtgatagaaa agcccttcat aagccagtct acttgttcag atgtgagagc
    42241 tttgacatcc ttttctgtac ttagccaagt cagttttccg ttctcaaagc gtttatatag
    42301 tagccaaaat ccttgaccat cccagtaaag gactttaaag cggtctttac gtccaccaca
    42361 aaagagaaag acttgaccgg agaaagaatc caattcaaag tgggttttaa ctacataggc
    42421 taatgagtct attccctgcc tcatatctgt cttgccacaa acaatgtgaa cttgacctaa
    42481 atcacttagt tgaatcatca tagtacaata cctttcctcc gataattatt ttttatctag
    42541 tatactggaa gttggggaat taggatagat accttgttat gacgcgctta ctatgaattt
    42601 gaagtatagt ctcctaaatg cacttagccc ttattatagg gctttttgtt ttaattattc
    42661 taatcgagtg agactggggg aaaaacaatt tcaggaaaaa tataagccct atacaaaaaa
    42721 ggaagcaaat tgcttccttt ctattattag ttattcaagg ctgctgccat tgtagctgca
    42781 acttcagctt cgaagtcgtt tgcagctttc tcgatacctt caccaacttc aaagcgagca
    42841 aactcaacta ccgaagcgtt aactgattca aggtatgctt caactgtctt gctgtcatcc
    42901 atgatgtaaa cttgtgcaag aagtgtgtaa gcttggtcaa ctttagtgtt atcaagcatg
    42961 aagcgatcca ttttacctgg aataattttg tcccagattt tttctggttt gccttctgca
    43021 gccaattcag ctttgatgtc agcttcagct tgagcaataa catcatcagt taattgagct
    43081 tttgatccat acttcaagtg tggaagagct ggtttattaa ccattgcacg gctttcgttg
    43141 tcttggtcga taacgtgatt caattgtgcc aactcatctt taacgaattg ctcatccaat
    43201 tctttgtaag aaagaactgt tggtttcatc gctgcgatgt gcattgacaa ttgtttagca
    43261 agtgcttcgt ctccaccttc aacaactgaa ataacaccga tacgtccacc gttatgttgg
    43321 tatgctccaa agtgttgtgc gtctgttttt tcaatcaatg caaagcgacg gaatgagatt
    43381 ttctctccga tagttgctgt tgcagatacg tatgcagctt caagagtttc acctgaaggc
    43441 attatcaaag caagagcttc ttcattgtta gcaggttttc cttcagcaat gactttagct
    43501 gtagtattta ccaattcaac gaattgagcg ttttttgcaa cgaagtcagt ttcagcgttt
    43561 acttcaataa ctgctgcaac attaccgtta acataaacac cagtcaaacc ttctgcagca
    43621 acacggtcag ctttcttagc tgccttagcc ataccttttt cacgaagcaa ttcaatcgct
    43681 ttttcgatgt caccgtctgt ttctacaagc gcttttttag cgtccataac accggcacca
    43741 gatttttcac gcaactcttt tacaagttta gctgtaattt ctgccatttt aattctccta
    43801 tattttttga aaataggaga gccgggctaa gccccgccct cctaggtaat tactatttgt
    43861 ataaattaag cgttgtcacc ttctacaact tcaacgattt cttcaattga atctgcttga
    43921 gtttctgaag ctgcaaattc tgcttcaact gctactgcat cctcaccttg acgtccttcg
    43981 ataatagcgt cagccaattt agctgtgatc aatttaacag cacggatagc gtcatcgtta
    44041 gctgggatga ttacatcgat atcatctgga tcagtattgg tgtcaaccat cgctacaact
    44101 gggattccca attttttagc ttctttaaca gcgatttgct ctttatgtgg gtcaactacg
    44161 tacatcacat ctgggatacg aggcatatct tcgataccac ccaagaattt ttcaagacgc
    44221 gcacgttgtt tgttaagaag tgcaacttct ttcttaggaa gaacttcgaa agttccatct
    44281 tcttccatac gtttaatttc tttcaaacga gcgatacgtt tttggattgt tccccagttt
    44341 gtaagagttc cacccaacca acggtggttg atgaagtatt gacctgaacg tactgcttct
    44401 tcagcaactg catcagctgc ttgtttctta gtaccaacga acaatacaac tgcatcgtta
    44461 gctgctgcat cacgcatgaa gtcgtatgct tggtcagcgt attttacagt ttgttgcaag
    44521 tcgataacgt ggattccgtt acgttcagta aagatgtact tagccatctt aggattccag
    44581 cgacgagttt ggtgaccaaa gtgtacacca gcctcaagaa gttgtttcat tgaaattact
    44641 gccatgagta tttctccttt ttgttttttt cctcttcttg acttcaactc gcaaaacgac
    44701 ccaagggcaa ctgcttcaca attcatcaag aatgagtatt atcgttcaca cgacaatttt
    44761 cattctacca tacttttcca atattttcaa gctattttga tataattttt aatagggatt
    44821 gtcacggtaa aacctgaaaa gagggctcta ttcgagtccc tctgtaaatt aatctgcata
    44881 aatatatgta acaaaacctt cagaagttgt tgttggattg aaccatccac ggtgatttcc
    44941 aattgtacga ttacctgcat aatttgattc tgatacttgg atacgtgttg ttgattcaac
    45001 agctgtaaca accgctacgt gaccatatcc accatcattc caacatgcaa ttgctccaac
    45061 ttgaggtgtt gaacctgtac ggaaacctgc tgctgctgca cttgtagccc actgtgctcc
    45121 attaccccag tagtctccag cccaaggtgc caatgttttt actccccatg tacattctcc
    45181 aattggataa cttgaagcgt ttgtactgta tgttggacga acttttgcac ggacaggtgc
    45241 tgctgcagat tcagatactg cttgcacttg agctgttaag ttagtgtttg ctgaagcaag
    45301 tactgattgt tgttggctag ctcgtttttc tttataagct gcttctgcta ccgcagctgc
    45361 acgagcctct gcctcagctg ctgctttttg ctctaataga cttgcttttt ccccttcagc
    45421 tgtcgctttc tcagcagcaa gacttaattc agcagctttt agttctgcct gtttcgtagt
    45481 caatgcttga gcatcatcag ccaatttttg ttgattagca attacagtat tgatagcatc
    45541 attatttgct acttgttttt cagaaatagc ttttttatct gccttttgtt gttctaacat
    45601 tttgttgttt gcagatacga tttcactcat tgcagcaaca cgtgaaatag cttctgtaat
    45661 tgattttgag tttacaatgg tattgatata gctagttacg gctccatttg tttgagcact
    45721 acgagcttgt ttttccaacg attggttacg agaaacaatg tttttagaaa gttctgtaat
    45781 ctcaccctcg agtttcttag attctgcttg taatctatca ttttcagctt gcaagttaga
    45841 ctgctcagct tgaatagctg atacttgctc ctgaatttgg tcaacttgtt tttgggcttc
    45901 ttgttgttgt gctgttaagt tactaatttt attatcttga gcagcaattt tgtcatcagt
    45961 cgtttctgca tgcgcagttg ttaaaacagc tacttgagaa accattactg tacttaataa
    46021 aagtgacgct aagatttttt tcttcatatt acgtagatac tccttctttt aaagacaata
    46081 c
//
LOCUS       DAVKDZ010000006        45596 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000006 DAVKDZ010000000
VERSION     DAVKDZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45596
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.006"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            114..1397
                     /gene="aroA"
                     /locus_tag="VWZ30_000332"
     CDS             114..1397
                     /gene="aroA"
                     /locus_tag="VWZ30_000332"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU9334138.1"
                     /translation="MKLKTNISHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVVTIQGVGMDGLKAPQNALDMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTIPLKKMGVSISGQTERDLPPLHLKGTKKLAPIQY
                     ELPIASAQVKSALMFAALQAQGESVIIEKECTRNHTEDMLQQFGAHLSVDGKKITVQG
                     LQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EVTEIDLVAKSATLTVESSDLQGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTVDGMIVKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVSDGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            1390..1866
                     /locus_tag="VWZ30_000333"
     CDS             1390..1866
                     /locus_tag="VWZ30_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU9334139.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGISIANFFA
                     EKGESAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIATDKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            1863..2711
                     /gene="pheA"
                     /locus_tag="VWZ30_000334"
     CDS             1863..2711
                     /gene="pheA"
                     /locus_tag="VWZ30_000334"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9334140.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQTVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAIAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAGKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            2708..3982
                     /gene="psr"
                     /locus_tag="VWZ30_000335"
     CDS             2708..3982
                     /gene="psr"
                     /locus_tag="VWZ30_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEU9334141.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSSPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTIDARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFGLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            4261..5106
                     /locus_tag="VWZ30_000336"
     CDS             4261..5106
                     /locus_tag="VWZ30_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9334142.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLICYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTVFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            5096..6199
                     /locus_tag="VWZ30_000337"
     CDS             5096..6199
                     /locus_tag="VWZ30_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9334143.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTINS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            6201..7187
                     /locus_tag="VWZ30_000338"
     CDS             6201..7187
                     /locus_tag="VWZ30_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9334144.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQRQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGYAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            7203..8672
                     /locus_tag="VWZ30_000339"
     CDS             7203..8672
                     /locus_tag="VWZ30_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEU9334145.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGVLARYFVARKKIKVANIGSSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNIQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVMIFVAKILTKENQYLLNKGEKNHV"
     gene            8665..9699
                     /locus_tag="VWZ30_000340"
     CDS             8665..9699
                     /locus_tag="VWZ30_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9334146.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPEDSAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            9899..10636
                     /gene="mecA"
                     /locus_tag="VWZ30_000341"
     CDS             9899..10636
                     /gene="mecA"
                     /locus_tag="VWZ30_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEU9334147.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            10787..12073
                     /locus_tag="VWZ30_000342"
     CDS             10787..12073
                     /locus_tag="VWZ30_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU9334148.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHSAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILATDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            12075..12944
                     /locus_tag="VWZ30_000343"
     CDS             12075..12944
                     /locus_tag="VWZ30_000343"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU9334149.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            13020..13097
                     /locus_tag="VWZ30_000344"
                     /pseudo
     CDS             13020..13097
                     /locus_tag="VWZ30_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            13239..14177
                     /gene="msrB"
                     /locus_tag="VWZ30_000345"
     CDS             13239..14177
                     /gene="msrB"
                     /locus_tag="VWZ30_000345"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9334150.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLL
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILSEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            14237..15961
                     /locus_tag="VWZ30_000346"
     CDS             14237..15961
                     /locus_tag="VWZ30_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9334151.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRNRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFLSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELAFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQELWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            15963..17711
                     /locus_tag="VWZ30_000347"
     CDS             15963..17711
                     /locus_tag="VWZ30_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9334152.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRGRII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDTFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            18013..19272
                     /locus_tag="VWZ30_000348"
     CDS             18013..19272
                     /locus_tag="VWZ30_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEU9334153.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKALGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    19348..19483
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            19522..20022
                     /locus_tag="VWZ30_000349"
     CDS             19522..20022
                     /locus_tag="VWZ30_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU9334154.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            20098..20466
                     /gene="rplL"
                     /locus_tag="VWZ30_000350"
     CDS             20098..20466
                     /gene="rplL"
                     /locus_tag="VWZ30_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU9334155.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DATDAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            20668..20841
                     /locus_tag="VWZ30_000351"
     CDS             20668..20841
                     /locus_tag="VWZ30_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334156.1"
                     /translation="MTKELQSSRHIVISFLVREMGIDIVEAISLMAELEKSGLVRLES
                     SGDLILKELGGAL"
     gene            20838..21617
                     /locus_tag="VWZ30_000352"
     CDS             20838..21617
                     /locus_tag="VWZ30_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEU9334157.1"
                     /translation="MKRITANQYQTSERYYKLPKILFEDEKYMDMKLEVKVAYSILKD
                     RLELSLSRGWIDEEGAVYLVFSNSKLMKLLGCSKSKLLSIKKTLKEYDLIDEVQQSSS
                     EKGRLANKIYLGELSSTPVGNSNRPSVKKRLGQDENDTAPVSHLAPSETEVSETKYSE
                     TDSLFIEDEEERNTQPILKRKVEKVTKYDRDYIWGLVQDQFRREGFSETASEIAMTDF
                     ERIYQYALDNVRFVRRAEVLAEFVFNGLYSVWNNRVRKGGG"
     gene            21716..23074
                     /gene="dcm"
                     /locus_tag="VWZ30_000353"
     CDS             21716..23074
                     /gene="dcm"
                     /locus_tag="VWZ30_000353"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEU9334158.1"
                     /translation="MRFIDLFSGIGGFRLGMESVGHECIGFCEIDKFARESYKSIFQT
                     EGEIEFHDIRDVSDDEFKKLRGKVDVICGGFPCQAFSIAGRRLGFEDTRGTLFFEIAR
                     AAKQIQPRFLFLENVKGLLNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPQNRE
                     RVFIIGHSRKKGTRLLFPFRREGQATNSETLKALGNLNPSKSGMSGKVYYSEGLAPTL
                     VRGKGEGFKVAIPCMTPDRLEKRQNGRRFKDNQEPMFTLNTQDRHGIVVVGDLPTSFK
                     ETGRVYGSEGLSPTLTTMQGGDKIPKILIPEPIQFLKVREATKKGYAQAEIGDSINLE
                     RPSSQYRRGRVGKGIANTLTTSGQMGVVVASYEGEDKQVYQVAGILIDGQFYRLRIRR
                     ITPKECFRLQGFPDWAFEAARKVSSNSQLYKQAGNSVTVPVIAAIAKKLKEIEEKDES
                     IK"
     gene            23058..23507
                     /locus_tag="VWZ30_000354"
     CDS             23058..23507
                     /locus_tag="VWZ30_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334159.1"
                     /translation="MKALNRESILDCDELETELHDAEIKQLDEQIFLMPNYPCEFEVT
                     FLDDYHKKHNYPLFYESYLQNIMEFLESQDIKNGADAFVDDNQNLVFVLYGQGYRAEG
                     KEGILTTQVTVKAYDEDKQPINFANLLDSLIVSEYQMEPNLWEVSHD"
     gene            23500..23880
                     /locus_tag="VWZ30_000355"
     CDS             23500..23880
                     /locus_tag="VWZ30_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334160.1"
                     /translation="MIDLYLSKNNHRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFIEMILKNPELTIRLPLAVSNKRVYPNLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            23893..24126
                     /locus_tag="VWZ30_000356"
     CDS             23893..24126
                     /locus_tag="VWZ30_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9334161.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRHYKMEQRMPQSPWVERIVQVLDLDYEDLFRR"
     gene            24129..24716
                     /locus_tag="VWZ30_000357"
     CDS             24129..24716
                     /locus_tag="VWZ30_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9334162.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKLLCIGELTILQILYGASLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGIGFISLLIISLCFSLIHAQGTTNQANLIGLQHQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTILAWCLYV
                     SLGMFLGLVRYKSDLWGSMGLHLVWNLSVYVLFLL"
     gene            25093..25407
                     /locus_tag="VWZ30_000358"
     CDS             25093..25407
                     /locus_tag="VWZ30_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEU9334163.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            25423..25809
                     /locus_tag="VWZ30_000359"
     CDS             25423..25809
                     /locus_tag="VWZ30_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEU9334164.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            25838..27220
                     /locus_tag="VWZ30_000360"
     CDS             25838..27220
                     /locus_tag="VWZ30_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU9334165.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLHL
                     EKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEYD
                     KLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKE
                     DLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKE
                     NTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGM
                     MFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQA
                     TCEAEVAGVD"
     gene            27223..27375
                     /locus_tag="VWZ30_000361"
     CDS             27223..27375
                     /locus_tag="VWZ30_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU9334166.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            27398..28816
                     /locus_tag="VWZ30_000362"
     CDS             27398..28816
                     /locus_tag="VWZ30_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEU9334167.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDALVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKKGYLSTIPVDRYPKKDIMGDKTVRVRADLHHIIKIETAKNGGNVKEVMEI
                     RLRSKLKSVLIVHYLKILYNRN"
     gene            28865..28948
                     /locus_tag="VWZ30_000363"
     CDS             28865..28948
                     /locus_tag="VWZ30_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEU9334168.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            29073..29810
                     /gene="erm(B)"
                     /locus_tag="VWZ30_000364"
     CDS             29073..29810
                     /gene="erm(B)"
                     /locus_tag="VWZ30_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEU9334169.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            complement(30067..31314)
                     /locus_tag="VWZ30_000365"
     CDS             complement(30067..31314)
                     /locus_tag="VWZ30_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9334170.1"
                     /translation="MSHNDSILNILGIKDKNIKIISVEEAEHNNDSVKEYITLITATL
                     SYPINRCRNCGFPTVNKDGFRKTHVRLASLNGRRYELELRKQRYKCKSCHTTFGAITN
                     LTKENQTLSSDLKNQIMLLARKGLSGQLIAEMCHCSPSSVRRTILERMEPHYRVAKLP
                     KHLCFDEFRSIKSVMSFICCDAETHQIVTKLQDRLSPTIVDYFESRYSKAERECVQSV
                     VIDLNAQYQSFIYRLFPNANIIIDRFHLVQLAGRALDNCRISILKQLDKQSQEYKIMK
                     SHWKLFHKKAEDLHPEEVVFLRGVKQYMTRQNAVDLITSKFSKFAEVYQTYQDITKAL
                     NERNSELLESTILDYQKTNTEMDTAIQTLRQNRKYVLNSAKFEYSNGPLEGINRKIKT
                     LKRTCYGFANQKFFFLRIDCIFS"
     gene            31494..31715
                     /locus_tag="VWZ30_000366"
     CDS             31494..31715
                     /locus_tag="VWZ30_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334171.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            31832..32329
                     /locus_tag="VWZ30_000367"
     CDS             31832..32329
                     /locus_tag="VWZ30_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEU9334172.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            32304..32810
                     /locus_tag="VWZ30_000368"
     CDS             32304..32810
                     /locus_tag="VWZ30_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU9334173.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            32794..35241
                     /locus_tag="VWZ30_000369"
     CDS             32794..35241
                     /locus_tag="VWZ30_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9334174.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            35244..37421
                     /locus_tag="VWZ30_000370"
     CDS             35244..37421
                     /locus_tag="VWZ30_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU9334175.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            37418..38419
                     /locus_tag="VWZ30_000371"
     CDS             37418..38419
                     /locus_tag="VWZ30_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEU9334176.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            38416..39348
                     /locus_tag="VWZ30_000372"
     CDS             38416..39348
                     /locus_tag="VWZ30_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU9334177.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            39593..39709
                     /locus_tag="VWZ30_000373"
     CDS             39593..39709
                     /locus_tag="VWZ30_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEU9334178.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            39725..41644
                     /gene="tet(M)"
                     /locus_tag="VWZ30_000374"
     CDS             39725..41644
                     /gene="tet(M)"
                     /locus_tag="VWZ30_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEU9334179.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            41763..41930
                     /locus_tag="VWZ30_000375"
     CDS             41763..41930
                     /locus_tag="VWZ30_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEU9334180.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(41990..42343)
                     /locus_tag="VWZ30_000376"
     CDS             complement(41990..42343)
                     /locus_tag="VWZ30_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9334181.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            42848..43270
                     /locus_tag="VWZ30_000377"
     CDS             42848..43270
                     /locus_tag="VWZ30_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9334182.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            43267..43497
                     /locus_tag="VWZ30_000378"
     CDS             43267..43497
                     /locus_tag="VWZ30_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9334183.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            43958..44161
                     /locus_tag="VWZ30_000379"
     CDS             43958..44161
                     /locus_tag="VWZ30_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEU9334184.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            44243..45460
                     /locus_tag="VWZ30_000380"
     CDS             44243..45460
                     /locus_tag="VWZ30_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9334185.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
BASE COUNT        14423 a         8148 c         9696 g        13329 t
ORIGIN      
        1 agtttcagaa ttgctaaaat aagaaaataa ctatcctaaa gtcaggaatt tttaaggaat
       61 ttctggcttt ttatgataaa ataggtaaaa gtattgcaag tatgaggtcc agtatgaaac
      121 taaaaacaaa cattagccac ttacatggca gtatccgcgt cccaggtgac aagtctatca
      181 gccaccgttc cattatcttt ggaagtttag ctgagggaga gaccaaggtt tatgatattt
      241 tgcggggtga agacgtcctt tcaaccatgc aggtctttcg tgatcttggt gtagagattg
      301 aggataaaga tggggttgtt accattcaag gtgtcggtat ggacggccta aaagctccac
      361 aaaatgccct tgatatggga aattctggaa cctcgattcg tctgatttca ggtgtccttg
      421 ctggtgcaga cttcgaagta gaaatgttcg gagacgatag tctttccaaa cgtcctatgg
      481 accgtgtgac gattccactg aaaaaaatgg gcgttagcat ttcagggcaa actgagcgag
      541 acttgcctcc ccttcactta aaagggacga aaaaactagc acctattcag tatgagttgc
      601 caattgcctc tgcccaagtc aagtcagcct tgatgtttgc ggccttgcag gctcagggag
      661 agtcagtgat tatcgaaaaa gaatgcaccc gtaatcatac tgaagatatg ctgcaacaat
      721 ttggtgctca tttaagtgtg gatggcaaga aaatcacagt ccaaggactt caaaaactga
      781 caggacaaaa ggtggttgtt ccaggagata tttccagtgc agccttttgg ttggtcgcag
      841 gtttgattgt tccgaattct cgtctagtgc tgcagaatgt ggggattaac gaaacgcgta
      901 ccggtattat tgatgtcatt cgtgccatgg gtggaaaatt agaagttact gaaattgacc
      961 tagtggctaa atctgctact ttgactgttg agtcttctga cttgcaagga acagagattg
     1021 gcggtgcctt gattccacgc ttgattgatg aattgcctat tattgccctt ttggcgaccc
     1081 aagcccaagg tgtaacagtt atcaaggatg ctgaggagct caaggtcaag gaaacagacc
     1141 gtattcaggt tgtggcagac gccttaaata gtatgggagc agatattact cctacggtag
     1201 atgggatgat tgtcaaagga aaatcagctc ttcacggtgc tagagtcaat acgtttggtg
     1261 accaccgtat cggcatgatg acagctatcg cagccctctt ggtttcagat ggagaggtgg
     1321 agcttgaccg tgcagaagcc atcaatacca gctatcctag tttctttgat gatttggaga
     1381 gcttgattca tggctaaggt gttattaggg tttatggggg ctggaaaatc gactattgca
     1441 agaggattgg accctaatta ccttgatatg gatgctctga ttgagaagcg cctaggtata
     1501 tccattgcga attttttcgc tgaaaaggga gaatcagcct ttcgtcaggt agaatcagaa
     1561 gtcctagctg atttactaca aacagaccaa gtcgtgtcaa ctggaggagg agtggttatt
     1621 tctcagagaa atcgtgactt actcaagact aatacagata acatctacct gaaagcagat
     1681 tttgaaaccc tctacctacg tatcgcaact gataaggaca atcagcgacc gctttttcta
     1741 aataatagca aggaagaact agtagctatt tttcaagaaa gacaggcttg gtatgaggaa
     1801 gtggctagtc gggttttgga tgtgaccaag ctaagcccag aggaaattat agaggaacta
     1861 agatgaaaat tgcttatcta ggtcccaagg gatcattttc acaccacgtt gtgcagacag
     1921 cttttcctca tgaggaattg caggcttttg ccaacattac agatgtcatc aaggcttatg
     1981 agcaaggatt agtggactat tctgtggtgc cagttgaaaa ctctattgag ggtagtgttc
     2041 atgaaacctt ggactatctt tttcatcagg ctcacatcca aacagtagca gaaatcgttc
     2101 agcctattca tcagcagttg atggtggttc caggccatac taagattgaa aagatttttt
     2161 cacatccaca ggcattggct caaggaaaga aattcatcga tgaacaatat ccagaggctc
     2221 aaatcgaggt aacagctagt acagcttatg cggcccgttt tatttccgaa catccagacc
     2281 agccctttgc agcaattgca cctagaagtt ctgctgaaga atatggattg gaactgattg
     2341 ccgaggatat tcaggaaatg gaagccaatt tcacacgttt ctgggttcta ggagctggaa
     2401 agcctagtat tcccttgcaa gcacaaactg aaaagatgag tttggccttg acattacctg
     2461 acaaccttcc aggtgcactt tataaggccc tgtcgacctt tgcttggcga ggaattgact
     2521 tgacaaaaat tgaaagtcgt ccactcaaga cagcactggg tgaatacttt tttattatcg
     2581 atgtggatta taccgataag gacttggtcc actttgccca aaaagaatta gaagcgattg
     2641 gaatccagta taaaattctg ggcgcctatc ctatttatcc aatatcagac catggaaagg
     2701 agagaagatg agtaaagaaa atcccttaag tcatcatgag cagttgcgtt atgattattt
     2761 gctaaaaaat attcactatc tcaatgagag agaaaaaaat gagtttgcct atttgcaaga
     2821 aaagctaact cttgctaggg gaaatagtag ctctagcttg gaacaagaaa gagaagagca
     2881 ggttgactta ccaagctatg cgaaccggag tcgctcacaa tccaaatcac aagcactctc
     2941 ttcacctcca aaaaagaaaa gacggaagct ccgtcttaaa cgaattttta tggtgatttt
     3001 ttcactttta gtctgtgtgg ctttggccat ggtattcatg tttttgcgtg gttaccaaga
     3061 tgctagcgca aagaaaacta ttgatgcccg ggcagctcaa gtagaagtct ttaatggtca
     3121 ggacactaga gatggagtta atattttaat catgggtact gatggtcgaa tcggccagaa
     3181 cagtgttgag acacgaactg actctattat ggtattaaat gtcgggggct cagataagaa
     3241 aatgaagcta gtcagtttca tgcgtgataa tttggtctat atagatggtt atagtcaagt
     3301 gattaacggt agaaaacaga cagataacaa gttaaacgta gcctacgagt taggagaaca
     3361 agaggggcaa aaaggggcag aaatggttcg ccaagtcttg aaagataatt ttggcttgga
     3421 cattaagtac tatgccttgg ttgatttcca ggcctttgcc acagcgattg acacgctttt
     3481 ccctgatggg gtgacgattg atgctcaatt ttcaacattg aatgggcgtc ccctaacaga
     3541 agctacagtc ggagatgatt tatacgctac cgagactgag tctccaaccc aaaccattaa
     3601 agtcggaaaa cagcagatga atggctcgac cctgctcaat tatgctcgtt tccgtgatga
     3661 tgatgaggcg gattacggcc gtacaaaaag acagcaacaa gttttaacag caattttaga
     3721 gcaaattaaa gatcctacta aacttttcac tggttctgaa gctcttggaa aggtttttgc
     3781 tatgacctca accaacgtac cctatacttt cctcctaaca aacggtttat ctgttttgga
     3841 tggagcaaaa aatggtattg aaaaattgac gattccagaa ctgggtgact gggtagatgc
     3901 ctatgatgtt tatggaggct tgggcttgct ggttgatcaa aacaaatatc aaaccaagct
     3961 cgctcaaatg ggcttgagat aagataatac tcaatgaaaa tcaaagtgca aactaggaag
     4021 ctagccgcag gctgctaaaa acacagtttt gaggttgtag ataagactga cgaagtcagt
     4081 tacctatacc tacggcaagg tgaagctgac gtggtttgaa gagattttcg aagagtataa
     4141 gacagaaaaa tcaaagtttg atagctattt ggatagcagt caggctttga ttttttacag
     4201 tctgcaatag attttcaccc cttgtttgtg gtataatgaa acgatacgat agaaagaata
     4261 atgaacaata tgactgattt aaaagcaatt caggctcgta gtctggagat ggctgaatat
     4321 tttgtggcct tttgtaaaga acatgatttg atctgttatc tctgtggcgg aggcgctatt
     4381 ggtgcccttc gaaacaaggg atttattcct tgggacgacg acctagactt ttttatgcct
     4441 cgtaaagatt atgagaaatt agcagaatta tggcctcgtt atgcagatga acgttatttc
     4501 ttgtcaaaga gtcacaagga ttttgttgat cgtaatcttt ttattaccat tcgtgacaag
     4561 gaaaccacct gtatcaagcc ttatcagcag gatttggatt tgccacatgg tctggccttg
     4621 gatgttttgc ctttggatta ttatccgaaa aatccagctg agcggaaaaa acaggttcgt
     4681 tgggccttga tttattcact cttttgtgcg caaactattc cagaaaagca tggtgctctt
     4741 atgaaatggg gaagtcgcat tttactgggt ttgactccaa aatctctccg ttatcgcatc
     4801 tggaaaaaag ctgagaaaga aatgactaag tatgatttgg ctgattgtga tggcattaca
     4861 gaattatgct caggtcctgg ctacatgaga aacaagtacc caatcacatc ttttgaagac
     4921 aatcttttct tgccatttga aggaacagag atgcctattc caatcggcta tgatgtctat
     4981 ctcagaactg tttttgggga ttatatgacg cctccaccag cagacaagca ggtaccgcat
     5041 catgatgctg tcatcgctga tatggataag tcttatacag aatacaaggg agaatatggt
     5101 ggctaagaaa aaaatcttat tttttatgtg gtctttttct cttggaggtg gtgcagagaa
     5161 gattctatca accattgttt caaatctgga tccagaaaag tatgatattg atattcttga
     5221 aatggagcac tttgacaagg gatatgaatc tgttccaaag catgtacgca ttttaaaatc
     5281 ccttcaagat tatcgccaaa ccagatggtt acgagctttt ttgtggagaa tgagaattta
     5341 ttttccaaga ctgactcgtc gtttgcttgt aaaagatgat tatgatgttg aagtttcttt
     5401 taccattatg aatccaccac tgttgttctc taaaagaaga gaagtcaaga agatatcttg
     5461 gattcatgga agtattgaag aacttcttaa ggatagctct aaaagagaat cacatagaag
     5521 ccagttggat gctgcgaata caattgtagg gatttcaaaa aagaccatca attctatcaa
     5581 ggaagtttat ccagattatg cttctaaatt acagacaatc tacaatggat atgattttca
     5641 gactattcta gaaaaatctc aagagaagat cgatatcgag attgctcctc aaagtatctg
     5701 tactatcgga cggattgagg aaaataaggg ttctgaccgt gtagtggaag tgatacgatt
     5761 attacaccaa gagggaaaaa actatcatct ctattttatc ggggctggtg atatggaaga
     5821 ggaactgaaa aaacgagtca aagagtatgg gattgaggac tatgtacatt tccttggtta
     5881 tcaaaaaaat ccttatcagt atttatctca gacgaaagtt ctcttgtcta tgtctaaaca
     5941 agaaggtttt cctggagtgt atgtggaggc cttgagtctg ggactccctt ttatctctac
     6001 ggacgttgga ggggctgagg aattatccca agaaggacga tttggacaaa tcattgagag
     6061 caatcaagag gcagctcagg cgattactaa ttacatgact tctgcctcaa actttgatgt
     6121 cgatgaggct agccaattca ttcaacaatt tacaattaca aaacaaatcg aacaagtaga
     6181 aaaactatta gaggagtagc atggaaactg cattaattag tgtgattgtg ccagtctata
     6241 atgtggcgca gtacctagaa aaatcgatag cttccattca gaggcagacc tatcaaaatc
     6301 tggaaattat tcttgttgat gatggtgcaa cagatgaaag tggtcgcttg tgtgattcaa
     6361 tcgctgaaca agatgacagg gtgtcagtgc ttcataaaaa gaacgaagga ttgtcgcaag
     6421 cacgaaatga tgggatgaag caggctcacg gggattatct gatttttatt gactcagatg
     6481 attatatcca tccagaaatg attcagagct tatatgagca attagttcaa gaagatgcgg
     6541 atgtttcaag ctgtggtgtc atgaatgtct atgctaatga tgaaagccca cagtcagcca
     6601 atcaggatga ctattttgtc tgtgattctc aaacatttct aaaggaatac ctcataggtg
     6661 aaaaaatacc tgggacgatt tgcaataagc taatcaagag acagattgca actgccctat
     6721 cctttcctaa ggggttgatt tacgaagatg cctattacca ttttgattta atcaagttgg
     6781 ccaagaagta tgtggttaat actaaaccct attattacta tttccataga ggggatagta
     6841 ttacgaccaa accctatgca gagaaggatt tagcctatat tgatatctac caaaagtttt
     6901 ataatgaagt tgtgaaaaac tatcctgact tgaaagaggt cgcttttttc agattggcct
     6961 atgcccactt ctttattctg gataagatgt tgctagatga tcagtataaa cagtttgaag
     7021 cctattctca gattcatcgt tttttaaaag gctatgcctt tgctatttct agaaatccaa
     7081 ttttccgtaa ggggagaaga attagtgctt tggccctatt cataaatatt tccttatacc
     7141 gattcttatt actgaaaaat attgaaaaat ctaaaaaatt acattagaac gggggtgacc
     7201 aagtgattga tgggaaacga ttattattta gtttgaccat agtcagttat gccttgacgc
     7261 tagtaagtgg aattgtgtat ctgtttaata ataataatgt tagcttactt tctactttat
     7321 tgttcttgtt ggttagtagc ttaattgctt gttggaatga tatcaagtat tacttaatcc
     7381 attttatttt ctatttaacc atttttgtat ttctggtatc aagaccgacc attgattatt
     7441 ttagggatgg tgctttggat acctatcatc caatagccta tcgttttgcc ttcatagttg
     7501 tcatggtttc gattctgggc ttgactacag gaggcgtcct ggctcgttac ttcgtagcta
     7561 ggaagaaaat aaaagtagca aatataggaa gttctctaaa agaggtttat atcaagcggt
     7621 tacgctttgt atcactagga gtttttcttc taacttatcc tttctatttc attcggttat
     7681 ttgaacggct cttgtatcgt ttgcagactt cctactatgc ctactatgca aattttgaaa
     7741 gtaaactgcc ttattttacc tacattttgt ctacctttac ggtctatgca atgtgtatgt
     7801 atctggcaac caagccaaag aaattgcagg ccacagcagt gcttgtctcc tttattgcag
     7861 ctaatactat tcatttggca attgggacac gaaatccctt tattttaagt attttatttg
     7921 cttttgttta ttactttatg cgggagcaaa ctgaaaaagg aaaatggatt gggtttaaag
     7981 aaaagttagc gatttttgta ggttctccta ttctcatgtt agcgatggga gtactcaatt
     8041 atgtacggga taatatccaa gtttcccata caggtttctg ggatatctta cttgacttta
     8101 tctataaaca agggactagt tttggtgttc tggctcgagg ttttctattt aacagtagcc
     8161 tcccttaccg agatttccgt aattttactt ttggtcctgt tcttgattat tttgcaaggg
     8221 ggagtttggg agccattttc ggaggaaaag cctttgaaca tacaaccaat agtgtggaac
     8281 tagctattga tagtaatagt tatgcccaca atctatccta tcttgtcttg aacaaggaat
     8341 acttgaaagg gcatggtatc ggaagtagtt atatcatgga gttgtatacc gactatggta
     8401 tgattggagt ctttctgctt agtttcttac tcggcgtatt atttatagcc atgctgcaag
     8461 tagcctatcg ctcaaggaca atcctatttg ctttatccct actcatcttg aataatctat
     8521 tctttatgcc aagaagcagc ttttcagaaa gtttcttcaa tttatttaca atgcaattct
     8581 ggggaattgt tcttgtgatg atattcgtag caaaaatact tacaaaagaa aaccagtatc
     8641 tactaaacaa aggagaaaaa aatcatgttt gaacattatt cagtagctga tttatttgca
     8701 aatctataca aaaaacgaaa agcaaatatt ctagctctca tcgctttatt tgctctcatt
     8761 gctgtaccat ttacaattaa agcagttagg aataaaaaca ctgtcaaaga tacaacaagt
     8821 tattcaactt atcttatcta taaaatcact cctccagaag attcggccaa aacgattttg
     8881 aatcatcaaa ttggtggtta tagtgatttt tatgggaaat tgattgatgg taatttgaat
     8941 ggagcttatc ttttcaatga tgtagaaccc agtgagttga aaaaaattgc cagtgaatta
     9001 gacacgacag aaacaacctt gaaaaattca acgaatgact attggtggaa aaaattgacc
     9061 gtctactata tgattgacga tgcaggggtt ggtgtgaaaa ttttgacatc aagtaaagat
     9121 gccaatgact tgttagagaa aaaaattgat gggttgattg agaaatttaa acatgcctat
     9181 gcaaatgtga aaattgaaaa actggaaacc atcaactcta aagaattgaa cgcaaatggt
     9241 gaaacagcgc ttggcttaaa tgtgaaaaat ctgattcttc gtttagttgt tattggagtg
     9301 gtttgtgtga ttttggttgt gatgggaaat gtgttagttt atctctttaa tccaacaatc
     9361 aatagagtag gtgatttttc tcagtatcaa attgattttg taacagagat tacaacaatt
     9421 gctaacctag cagatgtttt gtcatacaaa aatactggac aggaattgac catcgttagc
     9481 tcaaataaag ctatcctaga taaattgaaa cagagtcaag aagctttaaa aggaatgcat
     9541 tttgtagatt tacaggatgt atcatctctt ttggaaagag atacagtcct tcttgttgaa
     9601 gagtacggag tgactcgtta taagaaattt gagcaaagtc ttcaaattct cagaaactta
     9661 aatcgttcta tcctcggcgt agcaaccttt aaattataag tattctgatg tataaggtct
     9721 tcaaagtcgg ctaagagccg attttgaagg ctttttctat ctgtctgcag aaattttctt
     9781 ttggagtctc ctatagaaag accctaggga aaatctatcc gtatagagga gccttctttt
     9841 gataaaatcg taaaaatcta gtataataga tagaactgaa agtatgaggt tactagcaat
     9901 gaaaatgaaa caaattagtg atacaacttt aaaaatcacg atgtctttag aggatttgat
     9961 ggatcgtgga atggagattg ctgactttct cgttcctcaa gaaaaaacag aagagttctt
    10021 ttatgctatc ttggatgagc tagagatgcc tgatagcttt ctggatacag gcatgttgag
    10081 cttccgtgtg actccaaaac ctgataaggt agatgtcttt gtgaccaagt caaagattga
    10141 ccaaaatcta gattttgaag acttatcgga tttgccagat atggaagaat tggctcaaat
    10201 gtctccagat gaatttatca aaaccctgga aaaaagcatc gcagacaaaa ccaaggatga
    10261 tatcgaagcc attcaatctc ttgagcaagt tgaagccaag gaagaagagc aagagcaagc
    10321 tgaacaagaa gctgagagta agaaagaacc ttacatctac tacatccttt cttttgctaa
    10381 gttggctgac ttggtagctt ttgccaagac agtgactttt gaaatggaaa cttctgaact
    10441 ctacaaaatg aacgagcgct attatttgac cattttagtg gatattgaaa atcatccaag
    10501 cccatatcca gcttggctgt tggcccgtat gcgcgagttt gcagacgata gtgatatcag
    10561 tcgctcagtc ttacaagagt atggtcaagt cttgatgagt cacgatgcag tgctcaatct
    10621 gcaaaaaatc ggctaatcct ttctggaaag ctattttcag attttaagaa gagcgaatcg
    10681 tctgattcgt tttttctttt tatactgaaa tagtgattta ctataatagg aattttcaca
    10741 aaattctgtt ataatggcta tattagaaaa tttcgaggag ataaagatga cagttaaaat
    10801 tgctttacta ggatttggta ccgttgcaag tggtgtgcct ttcctcctaa aggaaaatgg
    10861 aggaaaaatc aatcaatcag cacattcaga tatcaaagtt gctaaggtat tggtcaagga
    10921 tgaagatgaa aaaaatcgct tgcttgcagc agggaatgac tttaactttg taaccaatgt
    10981 ggatgatatt ttatcagacc aagatattac tatcgtagtg gaattgatgg ggcgtattga
    11041 gcctgctaaa acctttatca ctcgtgcctt ggaagctgga aaacacgttg ttactgctaa
    11101 caaggacctt ttagctgtcc atagcgcaga attgctagaa atcgctcaag ctaacaaggt
    11161 agcactttac tacgaagcag cagttgctgg tgggattcca attcttcgta ctttagcaaa
    11221 ttccttggct tctgataaaa ttacgcgcgt gcttggagta gtcaacggaa cttccaactt
    11281 catggtgacc aagatggtgg aagaaggctg gtcttacgat gatgctcttg cggaagcaca
    11341 acgtctagga tttgcagaaa gcgatccgac gaatgacgta gatgggattg atgcagccta
    11401 caagatggtt attttgagcc aatttgcctt tggcatgaag attgcctttg atgatgtagc
    11461 ccacaaggga atccgcaata tcacaccaga agacgtagct gtagctcaag agcttggtta
    11521 cgtagtgaaa ttggttggtt ctattgagga aacttcttca ggtattgctg cagaagtgac
    11581 tccaaccttc ctacctaaag cgcacccact tgctagtgtg aatggcgtaa tgaacgctgt
    11641 ctttgtagaa tctatcggta ttggtgagtc tatgtactac ggaccaggtg cgggtcaaaa
    11701 accaactgca acaagtgttg tagctgatat tgtccgtatc gttcgtcgtt tgaatgatgg
    11761 taccattggc aaagacttca acgaatatag ccgtgacttg gtcttggcaa atcctgaaga
    11821 tgtcaaagca aactactatt tctcaatctt ggctacagac tcaaaaggtc aggtcttgaa
    11881 gttggctgaa atcttcaatg ctcaagatat ttcctttaag caaatccttc aagatggcaa
    11941 agagggtgac aaggcgcgtg tcgttatcat cacacacaag attaataaag cccagcttga
    12001 aaatgtctca gctgaattga agaaggtttc agaattcgac ctcttgaata ccttcaaggt
    12061 gctaggagaa taagatgaag attattgtac ctgcaaccag tgccaatatc gggccaggtt
    12121 ttgattcggt cggtgtagct gtaaccaagt atcttcaaat tgaggtctgc gaagaacgag
    12181 atgagtggtt gattgaacac cagattggca aatggattcc acatgatgag cgtaatctct
    12241 tgctcaaaat cgctttgcaa attgtaccag acttgcaacc aagacgcttg aaaatgacca
    12301 gtgatgtccc tttggcgcgc ggtttgggtt cttccagctc ggttatcgtt gctgggattg
    12361 aactagccaa ccaactgggt caactcaact tatcagacca tgaaaaattg cagttagcga
    12421 ccaagattga agggcatcct gacaatgtgg ctccagccat ttatggtaat ctcgttattg
    12481 caagttctgt tgaagggcaa gtctctgcta tcgtagcaga ctttccagag tgtgattttc
    12541 tagcttacat tccaaactat gaattacgta ctcgcgacag ccgtagtgtc ttgcctaaaa
    12601 aattgtctta taaggaagct gttgctgcaa gttctatcgc caatgtagcg gttgctgcct
    12661 tgttggcagg agacatggtg accgctgggc aagcaatcga gggagacctc ttccatgagc
    12721 gctatcgtca ggacttggta agagaatttg cgatgattaa gcaagtgacc aaagaaaatg
    12781 gagcctatgc aacctacctt tctggtgctg ggccgacagt tatggttctg gcttctcatg
    12841 acaagatgcc aacaattaag gcagaattgg aaaagcaacc tttcaaagga aaactgcatg
    12901 acttgagagt tgatacccaa ggtgtccgtg tagaagcaaa ataaagaata gaagatagga
    12961 tggggaaact cttgaccaga gggtttcata tcctttttgt gaaaagaagt ttatactcaa
    13021 tgaaaatcaa agagcaaact aggaagttag ccgcaggctg ctcaaaacag tgttttgagg
    13081 ttgtggatag aactgacgaa gtcagctcaa gacactgttt tgaggttgca gatagaactg
    13141 acgaaatcag taacatacct acggtaaggt gacgctgacg tggtttgaag agattttcga
    13201 agagtattag ttaaaaacgt gataaaggag aaataaagat ggcagaaatt tatctagcag
    13261 gtggttgttt ttggggccta gaggaatatt tttcacgcat ttctggagtg ctagaaacca
    13321 gtgttggcta cgctaatggt caagtcgaaa cgaccaatta ccagttgctc aaggaaacag
    13381 accatgcaga aacggtccaa gtgatttacg atgagaagga agtgtcactc agagagattt
    13441 tactttatta tttccgagtt atcgatcctc tatctatcaa tcaacaaggg aatgaccgtg
    13501 gtcgccaata tcgaactggg atttattatc aggatgaagc agatttgcca gctatctaca
    13561 cagtggtgca ggagcaggaa cgcatgctgg gtcgaaagat tgcagtagaa gtggagcaat
    13621 tacgccacta cattctgtct gaagactacc accaagacta tctcaggaag aatccttcag
    13681 gttactgtca tatcgatgtg accgatgctg ataagccatt gattgatgca gcaaactatg
    13741 aaaagcctag tcaagaggtg ttgaaggcca gtctatctga agagtcttat cgtgtcacac
    13801 aagaagctgc tacagaggct ccatttacca atgcctatga ccaaaccttt gaagagggga
    13861 tttatgtaga tattacgaca ggtgagccac tcttttttgc caaggataag tttgcttcag
    13921 gttgtggttg gccaagtttt agccgtccga tttccaaaga gttgattcat tattacaagg
    13981 atctgagcca tggaatggag cgaattgaag ttcgttctcg ttcaggcagc gctcacttgg
    14041 gtcatgtttt cacagatgga ccgcgggagt taggcggcct ccgttactgt atcaattctg
    14101 cttctttacg ctttgtggcc aaggatgaga tggaaaaagc aggatatggc tatctattgc
    14161 cttacttaaa caaataaaac agagagggtg gggtttccca ctttcttcat ttctagaata
    14221 tgaatagaag ggatttatga aacacctatt atcttacttc aaaccctaca tcaaggaatc
    14281 aattttagcc cccttgttca agctgttaga agctgttttt gagctcttgg ttcccatggt
    14341 gattgctggg attgttgacc aatctttacc tcagggagat caaggtcatc tctggatgca
    14401 gattggtctg ctccttatct ttgcagtaat tggcgtttta gtggccttga tagctcaatt
    14461 ttactcagca aaggcagcag taggttttgc taaggaattg acaaacgatc tttatcgtca
    14521 tattctttcc ttgcccaagg atagcagaaa ccgtctgaca acttctagtt tggtcactcg
    14581 cttgacttcg gatacctacc agattcagac tggtatcaat caattcctgc gtctcttttt
    14641 acgagcgccc attattgttt ttggtgccat ttttatggct tatcgaatct cagctgagtt
    14701 gactttctgg ttcttagtca tggttgccat tttgaccatt gtcattgtag ggttatctcg
    14761 attggtcaat cctctctaca gtagtctcag aaagaaaacg gaccaactgg ttcaggaaac
    14821 gcgccagcaa ttgcaaggga tgcgggttat tcgtgctttt ggtcaagaaa aacgagagtt
    14881 acagattttt caaaccctta accaagttta tgctagatta caagaaaaga caggtttctt
    14941 gtctagttta ttaacacctc tgacctatct gattgtcaat ggaactcttc tcgttattat
    15001 ctggcaaggc tatatttcaa ttcaaggagg agtgctcagt caaggtgctc tcattgctct
    15061 tatcaattac ctcttacaga ttttggtgga attggtcaag ctagccatgt tgataaattc
    15121 cctcaaccag tcctatattt cagccaagag aatcgaggag gtctttgcgg aatctcccga
    15181 aaacatccat tcagaattag aacaaaagca agttaccagt ggtcgggttt tacaagtcca
    15241 agaattggct tttacctatc ctgatgcggc ccagccttct ctgagagaca tttcctttga
    15301 tatgactcaa ggacaaatcc ttggtatcat tggggggact ggttctggta aatcaagctt
    15361 ggtgcaactc ttacttggac tttatccagt agacaagggg aacattgacc tttatcaaaa
    15421 tggacgtagt cctcttaatt tggagcagtg gcggtcttgg attgcctatg tacctcaaaa
    15481 ggtcgaactc tttaaaggaa ccattcgttc caacttgact ctaggtttca atcaagaagt
    15541 atctgaccag gaactctggc aggccttgga gattgcgcaa gctaaggatt ttgtcagtga
    15601 aaaggaagga ctcttggatg ctctagttga ggcagggggg cgaaatttct caggtggaca
    15661 aaaacaaaga ttgtctatcg cccgagcagt cttgcgccaa gctccgtttc tcatcctaga
    15721 tgatgcgacc tcagcactgg acaccattac agagtccaag ctgttgaaag ctattagaga
    15781 aaattttcca aacatgagct taattttgat ctctcaacga acctcgactt tacagatggc
    15841 ggaccagatt ctcctcttgg aaaaaggtga gttgctagct gttggcaagc acgatgactt
    15901 gatgaaatcc agtcaagtct atcgtgaaat caatgcatcc caacatggaa aggaggacta
    15961 gaatgaaacg acaaactgta aaccagacgc tcaaacgttt agccgtagat ttagcaaacc
    16021 atcccttcct ccttttccta gcctttctag gaactattgc ccaagttggc ttatcaattt
    16081 acctacctat tctgattggg caggtcattg accaagtcct agtggctggt tcatcaccag
    16141 ttttttggca gatttttctc cagatgctct tggtggtaat aggaaatact ctggtacaat
    16201 gggccaatcc tctcctctat aatcgtctaa tcttctctta taccagagat ttacgggggc
    16261 gaatcatcca taaactccat cggttaccga ttgcctttgt agataggcaa ggtagtggag
    16321 agatggttag tcgtgtaacc acggacatcg aacagttggc agctggcttg accatgattt
    16381 ttaaccaatt tttcattggt gttttgatga ttttggttag tattctagcc atgctccaaa
    16441 ttcatctcct catgactctc ttagtcttgc tattgacgcc actgtccatg gtgatttcac
    16501 gctttattgc caagagatcc tatcatctct tccagaagca aacagagacg agggggattc
    16561 agactcagtt gattgaagaa tcgcttagcc agcagactat aatccagtcc ttcaatgctc
    16621 aaacagaatt tatccaaaga ttgcgtgagg ctcatgacaa ctactcaggc tattctcagt
    16681 cagccatctt ttattcttca acggtcaatc cttcgactcg ctttgtaaat gcactcattt
    16741 atgccctttt agctggagta ggggcttatc gtatcatgat gggttcaacc ttgaccgtcg
    16801 gtcgtttagt gacttttttg aactatgttc agcaatacac caagcccttt aatgatattt
    16861 cttcagtgct agctgagttg caaagtgctc tggcttgcgt agagcgtatc tatggagtct
    16921 tagatagccc tgaagtggct gaaacaggta aggaagtctt gaccagtgac caagttaagg
    16981 gagctatttc ctttaaacat gtctcttttg gctaccatcc tgaaaaaatt ttgattaagg
    17041 acttgtctat cgatattcca gctggtagta aggtagccat cgttggtccg acaggtgctg
    17101 gaaaatcaac tcttatcaat ctccttatgc gtttttatcc cattagctcg ggagatatct
    17161 tgctggatgg gcaatccatt tatgattata cacgagcatc attgagacag cagtttggta
    17221 tggtgcttca agaaacctgg ctcacacaag ggaccattca tgataatatt gcctttggca
    17281 atcctgaagc cagtcgagag caagtaattg ctgctgccaa agcagctaat gcagactttt
    17341 tcatccaaca gttgccacag ggatacgata ccaagttgga aaatgctgga gaatctctct
    17401 ctgtcggcca agctcagctc ttgaccatag cccgagtctt tctggctatt ccaaagattc
    17461 ttatcttaga cgaggcaact tcttccattg atacacggac agaagtgctg gtacaggata
    17521 cctttgcaaa actcatgaag ggccgcacaa gcttcatcat tgctcaccgt ttgtcaacca
    17581 ttcaggatgc ggatttaatt cttgtcttag tagatggtga tattgttgaa tatggtaacc
    17641 atcaagaact catggataga aagggtaagt attaccaaat gcaaaaagct gcggctttta
    17701 gttctgaata agccattctc ttttgaaagt ttatggacga aaaaagttgc cttcgagtga
    17761 cttttttgtt acaatagcta gaaaaattgt tcactgtaat actcaatgaa aatcaaagag
    17821 caaactagga agctagccgt aggttgctca aagcacagct ttgaggttgt agataagact
    17881 gacgaagtca gttcaaaaca ctgttttgag gttgcagata gaactgacga agtcagtaac
    17941 cacacctacg gcaaagtgaa tctgaagtgg tttgaagaga gtacaacttg tcttttagaa
    18001 aaggagccta caatgaaagt ctttcagcat gtaaatatcg tgacttgtga tcaagatttc
    18061 catgtttatc ttgatggaat cttagcagtc aaggattctc aaatcgtcta tgtcggtcaa
    18121 gataagccag cgtttttaga gcaagctgag cagattatag actatcaggg agcttggatt
    18181 atgcctggtt tggtcaattg tcacacccat tctgcaatga caggtctgag agggatccga
    18241 gatgacagca atctccatga atggctcaat gactatatct ggccagcaga atctgagttt
    18301 actcccgaca tgactaccaa tgcggtcaaa gaagccctga cagagatgct ccagtcagga
    18361 acaacaacct ttaacgatat gtataatccc aatggtgtgg atattcagca aatttatcag
    18421 gtggtgaaaa cttccaagat gcgttgttat ttttctccga ctctcttttc ttcagagaca
    18481 gaaacaactg ctgagactat aagcagaact cgatccatca tagacgaaat cttaaaatat
    18541 aaaaatccaa atttcaaggt tatggtagca cctcattctc cgtatagctg cagtagagac
    18601 ttgctggaag cgagtttgga aatggcaaaa gagctaaata ttccgctcca tgtccatgtg
    18661 gcggagacca aggaggagtc aggaattatc ctcaaacgat acggcaaacg cccccttgct
    18721 tttcttgaag aactgggtta tttagatcat ccgtccgtat ttgctcacgg ggtcgaatta
    18781 aacgagagag aaattgaacg cttggcatct tctcaagtgg ctatcgccca caatcctatc
    18841 agtaacctca aactggcatc aggaattgct ccaattatcc agctccaaaa agcgggagta
    18901 gcagtcggaa ttgcgactga ctcggttgct tccaataaca atctagatat gtttgaggaa
    18961 ggaaggactg cagctcttct tcagaagatg aaaagtgggg atgccagcca gtttccaatc
    19021 gaaacagctc tcaaggtact gacaatcgaa ggggctaagg cccttggaat ggaaaatcag
    19081 ataggaagtc tggaagtcgg caagcaagca gattttctgg tcattcaacc acaagggaaa
    19141 attcatctcc aacctcagga aaatatgctg tctcacctgg tttatgcagt taaatctagt
    19201 gatgtagatg atgtttatat cgccggagaa caggttgtta agcaaggtca agtcctgaca
    19261 gtagaacttt aaaagaaaaa tcacgaaaaa ttttaaaaaa agtttgcaaa aatcttgcat
    19321 tctttttttg actatgctat acttatatac ggtttaaaaa aactgcctaa gacagtaggg
    19381 gagctcgact cataaatatc ctaccgagga caagacgtat catgtaaaaa gaagcgtatt
    19441 gtactttcgt gtctaggttt gggcgcgttt ttctttttga aaaattcccc aagcaaaata
    19501 attacggagg tgaacacact aatgagtgaa gcaattattg ctaaaaaagc ggaactagtt
    19561 gacgtagtag ctgaaaaaat gaaagctgct gcatctatcg tcgttgtaga cgctcgtggt
    19621 ttgacagttg agcaagatac agttcttcgt cgtgagcttc gtggaagcga agttgagtat
    19681 aaagttatta aaaactcaat cttgcgtcgt gcagctgaaa aagctggtct tgaagatctt
    19741 gcatctgtat ttgttggacc atctgcagta gcattttcta atgaagatgt tatcgcacca
    19801 gcgaaaatct tgaacgactt ttctaaaaac gctgaagcac ttgaaattaa aggtggtgca
    19861 atcgaaggcg ctgtcgcatc taaagaagag attcttgcac ttgcaactct tccaaaccgc
    19921 gaaggacttc tttctatgct cctttctgta cttcaagcgc cagtgcgcaa cgttgctctt
    19981 gcagtcaaag cggttgcaga aagcaaagaa gacgcggctt aatcttaagc tacatagcgt
    20041 agcctagcta cgaaaaaaac tattataaaa tttaaaactt atttggagga aataacaatg
    20101 gcattgaaca ttgaaaacat tattgctgaa attaaagaag cttcaatcct tgaattgaac
    20161 gaccttgtaa aagctatcga agaagaattt ggtgtaactg cagctgctcc tgtagctgtt
    20221 gctgcagctg atgcaactga tgctggtgct gctaaagatt cattcgacgt tgaattgaca
    20281 tctgcaggcg acaaaaaagt tggtgttatc aaagttgtac gtgaaatcac tggtcttggt
    20341 cttaaagaag ctaaagaact tgttgacggt gcaccagcac ttgttaaaga aggcgttgca
    20401 actgcagaag ctgaagaaat caaagctaaa ttggaagaag ctggagcttc agttactctt
    20461 aaataagagg catataccaa ttagaaatcg gaatactata gtatcagcct tttagaatcg
    20521 tgaacacatt ttagaaactg ataaattaat ttagtttcct accaaaaacc ctaccaaaaa
    20581 ttaatttggc agggtttttc tttttaacaa atttatcgtg gagaacaatt gaatattgtt
    20641 ctcctttttt tattttcagg aggaaaaatg acaaaagaat tacaatcatc acgccatatt
    20701 gtcatttcat ttttagtacg tgaaatggga attgacattg ttgaggccat ctctcttatg
    20761 gctgaattag aaaaaagtgg cttggttcgc ttggaatcaa gtggagattt aatactcaaa
    20821 gaacttggag gagcgctatg aaacgaatta ccgcaaatca ataccaaact tcagaacggt
    20881 attataaatt acctaaaatt ctttttgaag atgagaaata tatggatatg aaactagaag
    20941 taaaggtggc ttattctatt ttaaaagatc gtttagaatt atctctcagt cgtggttgga
    21001 tagatgaaga aggagcggtt tatttagtat tttctaattc taaactgatg aagctattag
    21061 gttgttcgaa gtcaaaatta ctgtccatca aaaaaactct taaagaatat gacttaattg
    21121 atgaagttca acagtcttca agtgagaagg ggagactagc taataagatt tatttagggg
    21181 aattatcttc taccccagta ggtaattcaa acaggcctag tgttaaaaaa agactagggc
    21241 aggatgaaaa tgatacggcc cccgtctcac atttagcccc tagtgagact gaagttagtg
    21301 agactaaata tagtgagact gattctttat ttattgagga tgaggaggag aggaatactc
    21361 aacctatctt gaaaagaaaa gtagaaaagg tcacaaaata tgatcgagat tatatttggg
    21421 gattggtaca agatcaattt agacgagagg ggttttctga aacagccagt gaaattgcta
    21481 tgactgattt tgagagaatc tatcagtatg ctcttgacaa tgttcgcttt gttagacgtg
    21541 cggaagtact tgctgaattt gtgtttaatg gcttgtattc tgtttggaac aaccgtgtta
    21601 gaaaaggagg tggttaaatg tcgccaatcg agtggatatt agttatgctc attgtcattt
    21661 caagtggagt gacagttctg acaatgatag tcgataagaa aggggtgata aagaattgag
    21721 atttattgat ttattttcag gtatcggtgg ctttcgactt ggaatggaaa gtgttggaca
    21781 cgagtgtatt ggattttgtg agatcgataa atttgctaga gaatcttata agtccatttt
    21841 tcaaacggaa ggagaaatag aatttcatga catacgagat gtttcagatg acgaatttaa
    21901 aaaacttaga gggaaagtcg atgtcatctg tgggggattc ccttgtcaag cattttcaat
    21961 cgcaggaaga cgattgggat ttgaagatac tagaggaact ttgttctttg aaattgctcg
    22021 ggcggccaaa caaatccaac cacgttttct ttttcttgaa aatgttaaag gcctactcaa
    22081 tcacgataag ggacggacgt tcaccacaat ccttaccaca cttgatgagt tggggtttga
    22141 tgttgagtgg caggtgctta acagtaagga ttttggcgtt ccccaaaaca gagagagggt
    22201 gtttattatc ggacattcta gaaagaaagg tacccgactc ctatttcctt tcagacgaga
    22261 aggtcaagca actaactctg agactttgaa agcactagga aatttgaatc catcaaaaag
    22321 tggaatgagt ggtaaagtct attattcaga aggtcttgcg ccaaccttag ttcgtggaaa
    22381 aggagaagga tttaaagttg cgattccttg tatgacacca gacagattag aaaagagaca
    22441 aaatggtaga cgtttcaagg ataatcaaga gccaatgttt actttaaata ctcaggatcg
    22501 ccatggtatt gtcgttgttg gagatttacc aactagcttt aaggaaactg gtcgcgtcta
    22561 tggaagtgag gggctatctc caacactgac tacgatgcaa ggtggagata aaatccccaa
    22621 aatactgatt ccagaaccaa tccaatttct aaaagtccga gaggcaacga aaaaaggata
    22681 tgctcaagca gagattgggg attcaatcaa tttagaaaga ccaagttctc agtatcgtcg
    22741 tggtagagtt ggaaaaggta tagcgaatac gttaacaact agtgggcaaa tgggagtagt
    22801 agtggctagc tatgaaggag aagataaaca agtttaccaa gtagctggta tcttaattga
    22861 tgggcaattt taccgtttga ggatacgacg aatcactcct aaagagtgtt ttagactgca
    22921 gggctttcct gattgggctt ttgaagctgc tagaaaagtc tctagtaata gtcagctcta
    22981 taaacaagct ggtaatagtg taaccgttcc tgtgattgcc gcaatcgcaa aaaaattaaa
    23041 agaaatagag gaaaaagatg aaagcattaa atagagaatc aatactagat tgtgatgaat
    23101 tagaaacaga attacatgac gcagaaatca aacagctgga tgaacaaata tttttaatgc
    23161 ctaattatcc atgtgagttt gaggtgacat ttttagatga ttaccataaa aaacataatt
    23221 acccactatt ttatgaatcc tatcttcaaa acattatgga attccttgaa agtcaggata
    23281 taaagaacgg agctgatgcc tttgtagatg ataatcagaa tctagttttt gttttatatg
    23341 gacaaggcta tcgagccgag ggaaaagagg gaatacttac aacccaagta actgtaaaag
    23401 cttatgatga agacaagcaa ccgattaact tcgcaaattt attagattcc ttaatcgtat
    23461 cagaatatca aatggaaccg aatctttggg aggtttccca tgattgatct ctatctaagt
    23521 aaaaataacc atagaaatca acttcttttg gatttcttcc aaaactatgg tatcgaggta
    23581 tcttgtcatt cagtttctga aatgacaaag gacaaattaa ttgagatgat gagctattct
    23641 tcagattgtt tcgagttttt atctcccaat ttattacgat ttaagaaccg tgacaattta
    23701 agattaacgg attttattga aatgatctta aaaaatcctg aactaaccat cagacttcct
    23761 cttgcggttt cgaataaaag agtttatcca aatctgaatc tggaggaggc tagagcttta
    23821 ttgccaagag atacgaaaca attgatttac atggcacaaa cacattattt atctaactaa
    23881 aggagaagca atatggctga acgattttgg gagaacttgt ccattatttt ggctgaaaga
    23941 aatattagct ggattgagtt aaccagaaaa atgtttgcgg gagagtttca ctatccaagt
    24001 gaattgaacc gtttgtatca aaagatacgc cactacaaga tggaacaacg aatgcctcaa
    24061 agtccatggg tagaaaggat tgtgcaggta ttagatttag attatgaaga tttatttcgg
    24121 aggtaactat gaaaagaatc attccagttt atatattcca acaagtaaat gtcctattgg
    24181 tatctctata cttactgaaa ttgctttgta tcggtgagtt aactatacta cagattctct
    24241 atggtgcgtc actcatttct tttttatgga tgtatggcca acgcaaacaa gctcataagg
    24301 tcaatatgaa atctaggatg aaatggcttg gtattggatt cattagccta ctgattataa
    24361 gtctatgttt tagcctaatc catgctcaag gaaccacgaa tcaagcaaac ttaattggac
    24421 ttcaacatca agttccttgg ttttcatttt tattgttctt aatcaatgcg agtatggttg
    24481 aagaatttct gtatcgagaa attttatgga acttggttag aaaattagat attcgagttg
    24541 ctttgacaag tgttttattt gccttagcac atcatccagg aaccattcta gcttggtgtt
    24601 tatatgtttc acttgggatg tttttagggc ttgtacgcta caaatcggac ttatggggca
    24661 gtatggggct acatttggtg tggaacctat cagtctatgt cttatttttg ctttaataat
    24721 agctgttctg tcttacgtat ggattaattt tgaaataaaa atagcataaa aatctagtta
    24781 tccgcataaa aactggactt atcacacttt atcaaggtca aaaccactca atttactact
    24841 aatttactac ttatgaatga gctttgatac gacgatttat ccttgaaaag tgaagatata
    24901 aagatacttc caataaaatt tgaatattta ataggtagac acttcaaaaa atgaggtgtc
    24961 tattttttta cccgattttg aaaggaagtg aacttatgaa aacaaaaaat caagaatcaa
    25021 aaggtcgttc cccactcttt aagaccatca aacattcatt cagccaataa aaaagaaagg
    25081 ataggtaaaa atatggaact taaatttgtg attcccaaca tggaaaaaac attcggcaat
    25141 ttagaatttg ctggcgagga taaagtcgtt cagcgaagaa tcaacggacg gctaactgtc
    25201 ttatcaagaa gctataatct ctattctgat gttcaaagag cagatgatat tgtggtggtg
    25261 cttcctgctg aagctggcga aaaacatttc ggctttgagg aacgtgtgaa gttagtcaat
    25321 ccacgtatta ccgcagaggg ctacaaaatc ggcactcgtg gttttacaaa ttacctttta
    25381 catgctgacg acatgataaa agaataaaga aagagaggaa aaatgatgag attagcaaat
    25441 ggcattgtat tagataaaga cacgactttt ggagaattga aattctctgc tctacgtcgt
    25501 gaagtgagaa tccaaaatga agacgggtcg gtttcagatg aaatcaagga acgtacctat
    25561 gacttaaaat ccaaaggaca aggacgcatg attcaagtaa gtattcctgc cagcgtgcct
    25621 ttgaaagagt ttgattataa cgcacgggtg gaacttatca atcccattgc ggacaccgtt
    25681 gctactgcca cctatcaagg agcagatgtt gactggtata tcaaggcaga cgatattgtg
    25741 ctgacaaagg attctagttc attcaaagct caaccacaag caaagaaaga accgacacaa
    25801 gacaaatagt cgctaggtag aaaggagact ttttcgcatg aaacagcgtg gtaaaaggat
    25861 tcgcccatct ggtaaagatt tagtctttca ttttacgata gcgtcactcc tgcctgtttt
    25921 cctgctggtt gtcggactgt ttcatgtgaa gacaatccag cagatcaact ggcaggattt
    25981 taacctatca caagcagata agattgacat tccctattta attatcagtt tcagtgtcgc
    26041 aattcttatc tgcttgctgg tagcgtttgt attcaaacgg gttcgctatg atacggttaa
    26101 acaactttac caccgtcaaa aactggcaaa gatgatactt gaaaacaagt ggtatgaatc
    26161 tgaacaggtc aaaacagagg gtttctttaa agatagtgct ggtcgtacaa aaaagataac
    26221 ctacttccct aaaatgtatt atcgacttaa aaatggcttg atacagatac gggtggaaat
    26281 cacgctggga aaatatcaag accaactctt acacttggaa aagaaattag agagtggctt
    26341 gtactgtgag ctgacggata aagagttaaa ggattcctat gtggaatata ctttgctcta
    26401 tgacaccata gccagtcgta tttctattga tgaagtagaa gctaaagatg gtaaacttcg
    26461 cttaatgaaa aacgtatggt gggaatatga taagctccct catatgttga ttgctggtgg
    26521 tacaggtggc ggtaaaactt actttatact gacactgatt gaagccttgc ttcatacaga
    26581 ttcaaaactg tatattcttg acccgaaaaa tgctgacctt gcggacttag gttctgtgat
    26641 ggcaaatgtc tactatagaa aagaagactt gctttcttgc attgaaacat tctatgaaga
    26701 aatgatgaaa cgtagtgagg aaatgaagca gatgaagaac tataagactg gcaaaaatta
    26761 tgcttactta ggtctcccgg cacacttctt aatctttgat gaatacgtcg ctttcatgga
    26821 aatgctggga acaaaagaaa acaccgcagt tatgaataag ctgaaacaga ttgtcatgtt
    26881 aggtcgtcaa gctggcttct ttctaatact ggcttgtcaa cgtccagacg caaaatattt
    26941 aggcgacgga atccgtgatc agtttaattt cagagtggct ttaggtcgta tgtctgaaat
    27001 gggctatggc atgatgtttg gcagtgacgt acaaaaggat ttcttcttaa agcgaatcaa
    27061 aggtcgtggc tatgttgatg taggaacaag tgtcatatca gagttttata ctccccttgt
    27121 accaaaagga tatgatttct tggaggaaat taaaaagtta tccaacagca gacagtccac
    27181 gcaggcgacg tgcgaagcgg aagtcgcagg tgtggactga tcttgctggc tggtgtggca
    27241 atagccacgc cagcacttaa ccccccgtat ctaacagggg ggtacaaatc gacaggaaac
    27301 agtcaaaaaa acattagaaa atcctttggt tacaagggat ttacaaaatt tcagcgtatg
    27361 tcaaatgggc tttaaaagtt gacatacgcc tttttgattg gagggatttt tactgaatga
    27421 acaaacttgg ttacagcatt taaaagaaaa acgcttggct tatggactat ctcaaaaccg
    27481 tttagctgtt gcgactggta ttacaaggca gtatctaagc gatattgaaa caggaaaagt
    27541 caagccatca gaggatttac agcagtccct ttgggaagct ctggaacgct tcaatcccga
    27601 cgctcccctt gaaatgctgt ttgattatgt aaggattcgc tttccgacaa cagacgtaca
    27661 gcaggtggtc gaaaacatct tacaactgaa actgtcctat tttcttcatg aggactatgg
    27721 tttctattct tattcagagc attatgcttt aggcgacata ttcgtccttt gctcccatga
    27781 actggacaaa ggagttctgg tggaattgaa aggtcgtggg tgcagacaat ttgaaagcta
    27841 tcttctggca caacaaagaa gctggtatga gttctttatg gacgctttgg tggctggcgg
    27901 tgtgatgaaa cgccttgacc ttgccattaa cgataagaca gggattttga atatccctgt
    27961 actcactgaa aagtgccaac aggaagaatg tatctccgtc ttccgcagtt ttaaaagcta
    28021 tcgcagtggc gaactggtac gcaaagagga aaaggaatgt atgggaaaca ccctctatat
    28081 cggttcatta caaagtgaag tttatttctg tatctatgaa aaggactacg agcagtacaa
    28141 gaaaaatgat attcccattg aagacgcaga agtaaaaaac cgttttgaga ttcgattgaa
    28201 aaatgagcgt gcctattatg cagtccgtga tttactcgtc tatgacaatc cagagcatac
    28261 cgcctttaaa attatcaatc ggtatatccg ttttgtagat aaagacgatt ccaaacctcg
    28321 ttctgattgg aaactgaatg aagaatgggc ttggtttatt gggaacaatc gtgaacgatt
    28381 aaaactaacc acaaaaccag agccttactc cttccaaagg acgctgaact ggctatctca
    28441 tcaagttgcc ccgaccttaa aggttgcgat taaacttgat gaaatcaacc agacgcaggt
    28501 tgtaaaagac attctcgacc atgcgaaact gacagaccga cacaagcaga ttttgaagca
    28561 acagtcagta aaagaacagg acgtgataac aacaaaaaaa ggatatctgt caaccatacc
    28621 agttgacaga tatccaaaaa aagatataat gggagataag acggttcgtg ttcgtgctga
    28681 cttgcaccat atcataaaaa tcgaaacagc aaagaatggc ggaaacgtaa aagaagttat
    28741 ggaaataaga cttagaagca aacttaagag tgtgttgata gtgcattatc ttaaaatttt
    28801 gtataatagg aattgaagtt aaattagatg ctaaaaattt gtaattaaga aggagggatt
    28861 cgtcatgttg gtattccaaa tgcgtaatgt agataaaaca tctactgttt tgaaacagac
    28921 taaaaacagt gattacgcag ataaataaat acgttagatt aattcctacc agtgactaat
    28981 cttatgactt tttaaacaga taactaaaat tacaaacaaa tcgtttaact tctgtatttg
    29041 tttatagatg taatcacttc aggagagatt acatgaacaa aaatataaaa tattctcaaa
    29101 actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa
    29161 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa
    29221 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgtcagaaa
    29281 aattaaaact gaatactcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc
    29341 ctaacaaaca gaggtataaa attgttggga atattcctta ccatttaagc acacaaatta
    29401 ttaaaaaagt ggtttttgaa agccgtgcgt ctgacatcta tctgattgtt gaagaaggat
    29461 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct
    29521 cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca aaagtaaaca
    29581 gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat
    29641 atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc
    29701 agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccatt acttatgagc
    29761 aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt
    29821 cgctttttta aatttggaaa gttacacgtt actaaaggga atggagataa attattagat
    29881 atactactga cagcttccaa gaagctaaag aggtccctag cgcctacggg gaatttgtat
    29941 cgataagggg tacaaattcc cactaagcgc tcgggacccc ttgtaggaaa atgtcctaag
    30001 tgggatatct gtcaactggt atggttgact aaaaatactt cctacgaaaa tgtagggggt
    30061 atttttttac gaaaaaatac aatcgattct taaaaagaaa aatttttgat tggcaaaacc
    30121 ataacaagtt cgttttaggg ttttgatttt gcgattgatg ccttctaaag gaccattaga
    30181 gtattcaaat ttagcgctat ttaagacata ttttctgttt tgacgaaggg tttgaatagc
    30241 agtatccatt tctgtattgg ttttttggta gtctaagatg gttgactcta gtaattcact
    30301 attgcgctcg tttagggctt tcgtgatatc ttggtaagtt tggtatactt cagcgaactt
    30361 ggaaaattta ctagtaatga gatcaacagc attttggcga gtcatatatt gtttaacgcc
    30421 gcgaagaaaa actacttctt cagggtgaag atcttcagct tttttatgga atagcttcca
    30481 atgtgacttc ataattttat attcttggct ctgtttatca agttgcttta ggatagagat
    30541 acgacaattg tccaaagcgc gaccagctaa ttgtacaagg tggaagcgat caataatgat
    30601 attggcatta gggaaaaggc gatagataaa actttgatat tgagcattta aatcaattac
    30661 aactgattga acgcattcgc gttcggcttt tgaataacga ctttcaaaat aatcaacaat
    30721 ggtaggtgat agacgatcct gtaactttgt gacaatttgg tgggtttcag cgtcacaaca
    30781 gataaaggac atcacagact taattgaacg aaactcgtca aaacatagat gcttaggcaa
    30841 cttagccaca cgatagtgtg gttccatgcg ctctaagatt gttcgacgaa cactgctagg
    30901 agagcagtga cacatttcag caataagctg accagataag cctttacgag ctaaaagcat
    30961 gatttgattt ttgagatcac tggataaggt ttgattttct ttggttaaat tagtaatagc
    31021 accaaaagta gtatggcatg atttacattt atagcgttgt ttacgaagct ctagttcata
    31081 tcttctccca tttaaacttg ccagtcgtac atgagttttg cgaaagccat ccttattaac
    31141 tgtgggaaag ccacagttac gacaacgatt aatcggataa gaaagagtag ctgttattag
    31201 cgttatatac tctttaacag aatcgttgtt gtgttcagct tcttcaacag aaataatttt
    31261 aatattttta tctttaattc caagaatatt taggatagaa tcattatggg acatttgttt
    31321 aaccttcttt catgattttt gtggtgaatt gattgtataa cgaggggaca gcaaatgtcc
    31381 tcttttttgt ataaaaaaat ctggcatgga atctctatcc ataccagaaa gtgtataccc
    31441 caaaaaaata actcaaatac aaattcattg aatatagaga ggagaacatt tttatgaatt
    31501 ttggacaaaa cctttataac tggtttctat caaacgctca atcactggtg cttttagcaa
    31561 tcgttgtgat tggcttgtat cttggcttca agcgtgagtt tagcaaactg attggctttt
    31621 taattattgc gattattgcg gttggcttag tcttcaacgc tgctggagta aaagacattt
    31681 tactagagct attcaatcgc attattggtg cttaaataaa accgttcttt tgtggaatat
    31741 aagtggtttt cttatgttcc gcaaaggaat ggtacaccaa acgaagtgcg gtagggattt
    31801 ttgaatctct acaaagaaag gacgtgaata tatggacgat atgcaagtct atattgcgaa
    31861 tttaggcaaa tacaatgagg gcgaattggt cggtgcgtgg tttacctttc ccattgactt
    31921 tgaggaagtc aaagagaaaa tcggcttgaa tgatgaatat gaggaatacg ccattcatga
    31981 ctacgagtta ccctttacgg ttgacgaata cacttccatt ggcgaactca atcgactatg
    32041 ggaaatggta tcggaattac ccgaagaatt acaatcggag ctatctgctc tgctcactca
    32101 tttttcaagc attgaagaac taagcgaaca tcaagaggat attatcattc attccgattg
    32161 tgatgatatg tatgacgtgg cacgctacta cattgaagaa acgggtgctt taggcgaagt
    32221 accagctagt cttcaaaact atattgatta tcaagcctat ggtcgggatt tagacctttc
    32281 aggaacgttt atctcaacca atcatgggat ttttgaaatc gtctattaaa tctgtcggta
    32341 cattactact ggcagatttt ctattttacg gggtggctca atcagctacc cctatttttt
    32401 atgaaaggat tgattacatg aagaaaatac gaagctatac cagtatctgg tctgtggaaa
    32461 aggtactgta ttctatcaat gattttagac ttccgtttcc cataaccttt acgcaaatga
    32521 catggtttgt cgtgtcactc tttgcagtga tgatacttgg caacttgccc cctctttcca
    32581 tgatagaggg agcatttctc aaatactttg ggattcctgt ggctttcaca tggtttatgt
    32641 ctacaaaaac ttttgatggt aaaaagcctt atggattttt gaagtctgtc attgcttatg
    32701 cactgcgacc aaagctgacc tatgcaggaa aaaaagtaac gcttggcaga aaccagccac
    32761 aagaagccat tacagcagtt aggagtgaat tttatggcat atccaattaa atacattgaa
    32821 aacaatctcg tctggaataa agacggggaa tgttatgctt actatgagct tgttccttac
    32881 aattactcat ttctcagtcc agaacagaaa atacaagtgc atgattcttt cagacagctt
    32941 atcgcacaaa atcgtgatgg caaaattcat gctttacaaa tcagtacaga atccagcata
    33001 cgttctgcac aagagcgttc caaaaatgaa gtcactggca agctcaaagc ggttgcctat
    33061 gacaaaatcg accaacagac agacgcttta atatccatga ttggcgaaaa tcaagtgaac
    33121 taccgtttct ttatcggctt taagttgctt ctcaacgatc aggagttttc tatgaaaagt
    33181 cttaccgttg aagcaaaaaa tgctttgtct gattttgtct atgatgtgaa ccataagctg
    33241 atgggcgatt ttgttagtat gagtaatgat gaaatcctgc gatttcagaa gatggaaaag
    33301 ctcttagaaa ataaaatctc tcgtcgtttc aaaatccgca ggttagataa ggacgacttc
    33361 ggctatctga ttgaacacct ttacggacag acaggcactg cctatgaaga gtatgagtac
    33421 catctatcaa agaaaaagct ggataatgaa acgctgatta aatactatga cttgattaag
    33481 cctactcgct gtttggtgga agaaaaacag cgatatttga aaatccagca ggaagatgaa
    33541 accgtctatg tagcttactt taccattaac agcattgtcg gagaactgga cttcccgtcc
    33601 tctgaaatct tctactacca gcaacagcaa tttacattcc cgattgatac gtcaatgaat
    33661 gtggaaattg tagcgaatcg taaagcccta tctactgtcc gcaataaaaa gaaagaactg
    33721 aaagacttgg ataaccacgc ttggcaaagt gataatgaaa ccagctccaa tgtggcggaa
    33781 gctctggaaa gtgtgaatga gctggaaacc aatttagacc aaagcaagga atctatgtac
    33841 aagctgtctt atgtggtaag ggtatcagca aatgatcttg acgaactcaa acgtcgttgt
    33901 aatgaagtga aagattttta tgacgattta agcgtaaaac tggtacgacc atttggggat
    33961 atgctcggct tacatgaaga atttttacct gccagcaagc gttatatgaa tgattatatt
    34021 caatacgtga cctctgattt cctcgctggt ttaggttttg gtgctactca aatgctgggg
    34081 gaaaatgagg ggatttatgt tggctacagc ttagatactg gacgcaatgt ctatctgaaa
    34141 cctgctcttg ccagtcaagg ggttaagggt tcagtaacca atgcgttagc gtcggctttt
    34201 gttggttcgc tgggtggtgg taaatccttt gcgaataacc ttatcgtcta ttatgcggtg
    34261 ctttatgggg cacaagcagt gattgtagac ccaaaagcag aacgtggcag atggaaagaa
    34321 accttgccag agatttccca tgaaatcaat atcgtcactc tgacttctga tgagaaaaac
    34381 aaaggcttac ttgaccctta tgtgattatg aaaaatccca aagattctga atcactggct
    34441 attgatattc tgacattcct tacggggatt tcctctcgtg atggggaacg cttcccaatc
    34501 cttagaaaag ccattcgtgc agtaaccaat agtgaagtac gagggttgat gaaagtgatt
    34561 gaggaattac gggttgagaa tacgccacta agtaccagta tagccgacca tatcgaaagt
    34621 tttacagact atgactttgc acatttatta ttcagtaatg gttatgtgga gcagtctatc
    34681 agcttagaaa aacaactgaa cattatacag gttgcggact tggtacttcc cgacaaggaa
    34741 acttcctttg aggaatatac cactatggag cttttatccg ttgctatgct gattgtcatt
    34801 agtacctttg ctttagactt tatccataca gaccgaagca ttttcaagat tgtagattta
    34861 gacgaagcat ggagcttttt acaggtagca caaggaaaaa cactatctat gaagctggtt
    34921 cgggctggtc gtgctatgaa cgctggggta tatttcgtga cccaaaatac agacgacctc
    34981 ttagatgaaa aactgaaaaa taacctcggc ttaaaatttg catttcgttc cactgacctt
    35041 aacgagatta aaaagacctt agcctttttt ggtgtagacc cagaggacga aaacaatcag
    35101 aagcgattgc gtgatttgga aaacgggcaa tgccttatca gtgatttata tggtcgtgtc
    35161 ggtgtgatac agttccaccc tgtatttgaa gaactgctcc atgcctttga taccagacca
    35221 cctgtgcgaa aagaggtgta aatgtgaaac catcaatagt aaacagaata aaatcaaact
    35281 ggacgctgaa acgtctaggt aaagtggcaa tgacagtggc tttcacactt gtgattgcca
    35341 tttttctttt agccatgctg ggaacggtgg ttcaagctgc gggcttggta gatgatacgg
    35401 tcaatgtggc aaatgaatac agccgatacc cacttgaaaa ctatcaactg gatttttatg
    35461 tggataatag ctggggctgg cttccgtgga actggtcgga cgggattgga aaacaggtca
    35521 tgtatggact atatgccatt accaatttta tttggacaat cagtttgtat gtttccaatg
    35581 cgacaggtta cttagtacag gaagcctatt ccttagactt catttccgct acagcagatt
    35641 ccattggtaa gaatatgcag accttagctg gtgtgagtgc aaacggattt tcaacagagg
    35701 gtttctatgt tggattcctc ttactcttga ttttggttct tggggtttat gttgcctata
    35761 cgggactgat aaagagagaa accacaaagg caattcatgc cattatgaat tttgtgctgg
    35821 tgtttatcct atcggcttcc tttattgcct acgctcccga ctacattaaa aaaatcaatg
    35881 acttttcatc agacatcagt aatgccagtt tatcacttgg cacgaagatt gtcatgcccc
    35941 attccgatag tcaaggcaag gacagcgtgg acttaatcag agatagcctg ttttccatac
    36001 aggttcagca accgtggcta ctgcttcaat acaacagttc agacattgaa agtatcggta
    36061 ttgaccgtgt ggaaagcctg ctctccacca gcccagattc caacaatggc gaagacagag
    36121 aaaaaattgt tgcggaagaa attgaagaca gaagcaatac caatctaacc attacaaaga
    36181 ccattaaccg tttaggtaca gtcttcttcc tatttgtctt caatattggg atttccatat
    36241 ttgtattcct attaacagga atcatgattt tctcgcaggt actttttatc atctatgcta
    36301 tgtttctgcc tgtgagcttt attttaagca tgattccatc atttgatggt atgtcaaaac
    36361 gagccataac aaagctcttt aataccattt tgacacgagc tggaatcaca ttgattatta
    36421 cgacagcatt tagtatttca accatgctct ataccttatc ggctggttat ccgttctttt
    36481 tgattgcttt tctacagatt gtgacctttg caggaatcta cttcaagctg ggcgatttaa
    36541 tgagtatgtt ttctctacag agtaacgatt ctcaaagtgt gggaagtcgt gtgatgagaa
    36601 aacctcgtat gcttatgcac gctcacatgc accgtctaca gcggaaactt ggacgttcca
    36661 tgactactct aggggctggg tctgccattg ttacaggtaa aaaaggacag tcgggttcgg
    36721 ggagttctgc aaggacacaa gcagatcact cccgaccaga cggaaaggaa aaatcaacac
    36781 ttggaaaacg tatcggtcaa accatcggta cagtagctga taccaaagac agaatggtag
    36841 acactgctag tggtttgaaa gaacaggtta aagatttgcc gaccaatgca agatatgcag
    36901 tatatcaagg aaaatccaaa gtaaaagaga atgtccgtga tttaaccagt agtatttctc
    36961 aaaccaaagc ggacagagcc agtggacgca aggaacagca ggaacaaagg cgaaaaacca
    37021 ttgcgaagcg tcgctctgaa atggaacagg tcaaacagaa aaaacagcct gcttcttctg
    37081 ttcatgaaag accgactaca agacaagaac aatatcatga tgaacagacc tcaaaacagt
    37141 ctaatattca gacttcatat aaggaatctc aacaagccaa acaagagcgt ccagcagtta
    37201 agtccgattt ttcaagtcca aaagtggaac gccaaggcaa taccgttcaa gaaaaaaccg
    37261 ttcaaaagcc agcaacttca accactacag cagatagaac ttcacaacgt ccaatcacaa
    37321 aagaacgtcc gtctactgtt caaagagtac cactacaaaa tacaagaagt agaccaccaa
    37381 tcaaaaccgc caccattaag aaagtcggta agaaaccatg aagttgaaaa ctttagtgat
    37441 tggtggttct ggattattct tgatggtctt ctcactgctt ctgtttgttg ccattttatt
    37501 ttcagatgaa caggacagcg gaatttccaa tattcattat ggaggtgtga atgtttccgc
    37561 agaagtgctg gctcataagc ctatggtaga aaaatatgcc aaagaatatg gcgttgaaga
    37621 atatgtcaac atacttcttg cgattataca ggtggaatcg ggcggtactg cggaagatgt
    37681 tatgcagtcc tcggaatccc tcggtcttcc acctaattca ttgagtacag aagaatccat
    37741 taagcaaggt gtgaagtatt tcagtgaatt attagccagt agcgaaaggc tcagtgtaga
    37801 tttagaatcg gttatccagt cctacaatta tggtggtggt ttcttagggt atgtggctaa
    37861 tcgtggaaat aaatatacct ttgaactggc tcaaagtttc tcaaaagagt attcaggtgg
    37921 cgaaaaagtg tcttacccca atcccatagc catacctatc aatgggggct ggcgatacaa
    37981 ctatggcaat atgttttatg tgcaactggt aacgcagtat cttgtcacaa cagagtttga
    38041 tgatgatacg gtacaagcca tcatggacga agcactgaaa tatgagggct ggcgatacgt
    38101 ttacggtgga gcttccccga ctacttcttt tgattgtagc ggactgacac aatggacgta
    38161 tggaaaagct ggaattaact taccacgaac cgcacaacag caatatgatg tgacccagca
    38221 tatcccacta tcggaagcac aagctggcga tttggttttc tttcattcta cctataacgc
    38281 tggctcttat attactcatg ttgggatata ccttggcaat aaccgtatgt ttcatgcagg
    38341 cgacccaatc ggttatgccg acttaacaag cccctactgg caacagcatt tagtgggagc
    38401 aggacgaatc aaacaatgag aaaggaagat ttaatgatga aatttagaaa aaatcagaat
    38461 aaagaaaaac agataccaaa ggaaaagaaa cctcgtgtct ataaggtcaa tcctcataaa
    38521 aaggttgtga ttgccttgtg ggtactttta gggcttagtt tcagctttgc gatattcaag
    38581 cactttacag ctatagatac tcatactatt cacgaaacaa ctatcataga aaaggaatac
    38641 gttgatactc atcatgtaga aaattttgta gagaactttg cgaaagtcta ctattcatgg
    38701 gagcaatccg ataagtccat tgataatcga atggaaagtc taaaaggcta tctgacagat
    38761 gaacttcaag ctctcaatgt tgatacagta cgcaaagata ttcctgtatc gtcttctgta
    38821 agaggatttc agatatggac ggtagagcca actggcgaca atgagtttaa tgtaacctac
    38881 agtgtagacc agctcattac agagggagaa aatacaaaga ccgtccactc tgcttatata
    38941 gtgagtgtct atgtagatgg ttctggaaat atggtactgg ttaagaatcc gaccattacc
    39001 aacataccta agaaatcaag ttataaacca aaagccattg aaagtgaggg gacggttgat
    39061 tccattacaa ccaatgaaat caatgagttt ttaacgacgt tcttcaagct ctatcctaca
    39121 gcgacagcca gtgaactttc ctactatgtg aatgacggga tattaaaacc aatcggaaaa
    39181 gagtacatct ttcaagaact ggtaaatcct attcacaatc gtaaggataa tcaagtcacg
    39241 gtatcgctga cagtggagta tatcgaccag cagaccaaag caacgcaggt atctcaattt
    39301 gatttggtac ttgaaaagaa cgggagtaat tggaagatta tagaataaca aatattggta
    39361 cattattaca gctattttgt aatcacgtac tctctttgat aaaaaattgg agattccttg
    39421 acaaatatgc tcttacgtgc tattatttaa gtatctattt aaaaggagtt aataaatatg
    39481 cggcaaggta ttcttaaata aactgtcaat ttgatagtgg gaacaaataa ttggatgtcc
    39541 ttttttagga gggcttagtt ttttgtaccc agtttaagaa tacctttatc atgtgattct
    39601 aaagtatccg gagaatatct gtatgctttg tatgcctatg gttatgcata aaaatcccag
    39661 tgataaaagt atttatcact gggattttta tgcccttttg ggtttttgaa tggaggaaaa
    39721 tcacatgaaa attattaata ttggagtttt agctcatgtt gatgcaggaa aaactacctt
    39781 aacagaaagc ttattatata acagtggagc gattacagaa ttaggaagcg tggacaaagg
    39841 tacaacgagg acggataata cgcttttaga acgtcagaga ggaattacaa ttcagacagg
    39901 aataacctct tttcagtggg aaaatacgaa ggtgaacatc atagacacgc caggacatat
    39961 ggatttctta gcagaagtat atcgttcatt atcagtttta gatggggcaa ttctactgat
    40021 ttctgcaaaa gatggcgtac aagcacaaac tcgtatatta tttcatgcac ttaggaaaat
    40081 ggggattccc acaatctttt ttatcaataa gattgaccaa aatggaattg atttatcaac
    40141 ggtttatcag gatattaaag agaaactttc tgccgaaatt gtaatcaaac agaaggtaga
    40201 actgtatcct aatatgtgtg tgacgaactt taccgaatct gaacaatggg atacggtaat
    40261 agagggaaac gatgaccttt tagagaaata tatgtccggt aaatcattag aagcattgga
    40321 actcgaacaa gaggaaagca taagatttca taattgttcc ctgttccctg tttatcacgg
    40381 aagtgcaaaa aacaatatag ggattgataa ccttatagaa gtgattacga ataaatttta
    40441 ttcatcaaca catcgaggtc agtctgaact ttgcggaaaa gttttcaaaa ttgagtattc
    40501 ggaaaaaaga caacgtcttg catatatacg tctttatagt ggcgtactgc atttgcgaga
    40561 ttcggttaga gtatcagaaa aagaaaaaat aaaagttaca gaaatgtata cttcaataaa
    40621 tggtgaatta tgtaagattg atagagctta ttctggagaa attgttattt tgcaaaatga
    40681 gtttttgaag ttaaatagtg ttcttggaga tacaaaacta ttgccacaga gaaaaaagat
    40741 tgaaaatccg caccctctac tacaaacaac tgttgaaccg agtaaacctg aacagagaga
    40801 aatgttgctt gatgcccttt tggaaatctc agatagtgat ccgcttctac gatattacgt
    40861 ggattctacg acacatgaaa ttatactttc tttcttaggg aaagtacaaa tggaagtgat
    40921 tagtgcactg ttgcaagaaa agtatcatgt ggagatagaa ctaaaagagc ctacagtcat
    40981 ttatatggag agaccgttaa aaaatgcaga atataccatt cacatcgaag tgccgccaaa
    41041 tcctttctgg gcttccattg gtttatctgt atcaccgctt ccgttgggaa gtggaatgca
    41101 gtatgagagc tcggtttctc ttggatactt aaatcaatca tttcaaaatg cagttatgga
    41161 agggatacgc tatggttgcg aacaaggatt atatggttgg aatgtgacgg attgtaaaat
    41221 ctgttttaag tacggtttat actatagccc tgttagtacc ccagcagatt ttcggatgct
    41281 tgctcctatt gtattggaac aagtcttaaa aaaagctgga acagaattgt tagagccata
    41341 tcttagtttt aaaatttatg cgccacagga atatctttca cgagcataca acgatgctcc
    41401 taaatattgt gcgaacatcg tagacactca attgaaaaat aatgaggtca ttcttagtgg
    41461 agaaatccct gctcggtgta ttcaagaata tcgtagtgat ttaactttct ttacaaatgg
    41521 acgtagtgtt tgtttaacag agttaaaagg gtaccatgtt actaccggtg aacctgtttg
    41581 ccagccccgt cgtccaaata gtcggataga taaagtacga tatatgttca ataaaataac
    41641 ttagtgtatt ttatgttgtt atataaatat ggtttcttgt taaataagat gaaatatttt
    41701 ttaataaaga tttgaattaa agtgtaaagg aggagatagt tattataaac tacaagtgga
    41761 tattgtgtcc tgtatgtgga aataaaacac gattaaagat aagggaagat actgaattaa
    41821 aaaaattccc cctctattgt ccgaaatgca gacaagaaaa tttaattgaa ataaagcagt
    41881 tcaaagtaac tgtgattaca gagccagacg caaagacgca gagccgataa aatgagatta
    41941 atacaatctc attttatcgg ctctttccgt tatgtatgga ttcttttaat tagtcttcga
    42001 tgtttcttgc ttcgttgata ccgctggcta aagattccat taaggatagt tctttgtctg
    42061 taaagctatc catgtatttc tctatctgta atcgtcgggt gctttttacc aagttattag
    42121 caggtaagaa aaattcatca acggaaacat gaagtaacga tacaaggtca taaagaactt
    42181 gtatgctggg gtgttgccct ttattttcaa tattagttaa gtaccgtggg tcaatttcaa
    42241 tcaatgctcc cacttgttca cgagttaaac ctcgtttcaa tcgagcttct ttaatggcta
    42301 aaccaaaggc tctaaaatca tatttatctt cttttttacg catagtagac cacctctata
    42361 cattttattg ttcctactga attaaaaaca ggtatagaaa aacgtgttat atggtttata
    42421 ggtttatatt taataaaaag cactactaaa cgccaataaa aaaaaccgtt atatggtagt
    42481 gctatttacg ctgttaaaat attgtatatt acttccaaat ggcggtttgt tggaggtcaa
    42541 cgtcgccatg aagtacatca tatacaataa atttccttac attgggttct tgtcaaaaaa
    42601 agtcgtctat ctgcaataga taagtacgtc caccaatgtg gttttataaa tcatatagat
    42661 agaataacag aagcatgtaa acagagaaat aaatctgttt atatgctttt ttggctattc
    42721 agaacttttt tacaaagttt atttatcagt aatgcaacaa atcccccttt cacattggga
    42781 ctaagagtga aaggagataa acgagcaagg ctcacttcct ttcctagaca gaaagggggt
    42841 gagaaacatg aaaccatctt cttttcagac cacaatagaa aatcagtttg actatatctg
    42901 taaacgtgct atggaagacg agcgaaagaa ttatatgctt tatctttcaa ggattgcaaa
    42961 gcgtgaggtg tccttttcgg atgttggcga ttatcttgtt agccagtttg cgacaacaga
    43021 taactattca actgactttc agatttttac actcaatggg ttatcagtag gcgttgaaaa
    43081 tgatttgttg agtgaagcat tacgtgagtt gccagacaag aaacgtgaaa ttctactgct
    43141 gttttacttt atggacatga gcgattcaga aattgcagac ctgttgaaat tgaaccgttc
    43201 tactgtctat cggcatagaa ccagtggact agccttaatt aaaaagttta tggaggaatt
    43261 tgaagaatga aaacacaata tcctatgatt ccctttcctc tcattgtaaa ggcaacagat
    43321 ggcgataccg aagcgattaa ccagattcta catcattaca gagggtacat aacgaagcgt
    43381 tccctacgac ttatgaaaga tgaatatggc aatcaaagta tggtcgttga tgaagtctta
    43441 cgtggaagaa tggaaaccag actgattaca aagattttgt catttgaaat taagtaatat
    43501 cctctctcct ttcgtggaag cgtgctaaac cattccacgc ttcccgaaca gggaggtttg
    43561 ttattccacc aaagcatatt gagctttcaa tgtgttttga taggctaacg agccattgtt
    43621 ctttgaaaac tgaataaaag taatcgaata cgtttcgata agaaaagagc caacggaact
    43681 aaccgccatg acctatctta taaagatagc gagcgattca tgttagtgat ccgagaagca
    43741 atctttagca ggattgcctg caacgacatt cttatcgtga taatgatact cccatacagt
    43801 caatagtccg agcgtgataa aaccgtcgca ggcaatgagt atggctacat gagaaccatg
    43861 caggggtgga actcccgtga gctttgctaa agctgttcga ttgctggtaa aacaactttt
    43921 atgaaatcca aataagtgat ttggaaagga ggattttatg aagcagactg acattcctat
    43981 ttgggaacgt tataccctaa ccattgaaga agcgtcaaaa tattttcgta ttggcgaaaa
    44041 caagctacga cgcttggcag aggaaaataa aaatgcaaat tggctgatta tgaatggcaa
    44101 tcgtattcag attaaacgaa aacaatttga aaaaattata gatacattgg acgcaatcta
    44161 gcgtcgccaa agggtcttgt atatgataaa atagtattaa gtcgtatcaa ggctctttcc
    44221 ataaaggaaa ggagcaaatg ccatgtcaga aaaaagacgt gacaataaag gtcgaatctt
    44281 aaagactgga gagagccaac gaaaagacgg aagatactta tacaaatata tagattcatt
    44341 tggagaaccg caatttgttt actcgtggaa acttgtggct acagaccgag taccagcagg
    44401 aaagcgtgat tgtatctcac ttagagagaa aatcgcagag ttacagaaag acattcatga
    44461 tggtattgat gttgtaggaa agaaaatgac actctgccag ctttacgcaa aacagaacgc
    44521 tcaaagacca aaggttagaa aaaacactga aactggacgc aaatatctta tggatatttt
    44581 gaagaaagac aagttaggtg taagaagtat tgacagtatt aagccatcag acgctaaaga
    44641 atgggctatt agaatgagtg aaaatggtta tgcttatcaa accatcaata actacaaacg
    44701 ttctttaaag gcttcattct atattgctat acaagatgat tgtgttcgga agaatccatt
    44761 tgactttcaa ctgaaagcag ttcttgatga tgatactgtc cctaagaccg tactaacaga
    44821 agaacaggaa gaaaaactgt tagcctttgc aaaagctgat aaaacctaca gcaaaaatta
    44881 tgatgaaatt ctgatactct taaaaacagg tcttcgtatt tcagagtttg gtggtttgac
    44941 acttccagat ttagattttg agaatcgtct tgtcaatata gaccatcagc tattgagaga
    45001 tactgaaatt gggtactaca ttgaaacacc aaagaccaaa agtggcgaac gtcaagttcc
    45061 tatggttgaa gaagcctatc aagcatttaa gcgagtgtta gcgaatcgaa agaatgataa
    45121 gcgtgttgag attgatggat atagtgattt cctctttctt aatagaaaga actatccaaa
    45181 agtggcaagt gattacaacg gcatgatgaa aggtcttgtt aagaaataca ataagtataa
    45241 cgaggataaa ttgccacaca tcactccaca tagtttgcga catacattct gtaccaacta
    45301 tgcaaatgca ggaatgaatc caaaggcatt acagtacatt atgggacatg ctaatatagc
    45361 catgacgctg aactattacg cacatgcaac attcgattct gcaatggcag aaatgaaacg
    45421 cttgaataaa gagaagcaac aggagcgtct tgttgcttag tagtacaaat gaatttacta
    45481 cttatttacc acttctgaca gctaagacat gaggaaatat gcaaagaaac gtgaagtatc
    45541 ttcctacagt aaaaatactc gaaagcacat agaataaggc tttacgagca tttaag
//
LOCUS       DAVKDZ010000007        44494 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000007 DAVKDZ010000000
VERSION     DAVKDZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44494
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.007"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(<1..562)
                     /locus_tag="VWZ30_000381"
     CDS             complement(<1..562)
                     /locus_tag="VWZ30_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334186.1"
                     /translation="MSDIRDSLDKILAVSIDSIKDEQNFLNLVLSDSPEGNILKELAI
                     FKDNGALYKLIFSVYATFEYTIKECCNTALLTIDKNSLDSLTDELQMLTFRQKLDELR
                     KKIIEKREDNTVIKPLTDLHIKIKQQTEFNSNENMIDTKSNLNFNNFTEILEIFHFDK
                     KKYKSYSIIIDSIITYRNMIAHGNRKD"
     gene            complement(555..1652)
                     /locus_tag="VWZ30_000382"
     CDS             complement(555..1652)
                     /locus_tag="VWZ30_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEU9334187.1"
                     /translation="MAQNKLVRRNFDISFNELYDMYNDGELVIKPEFQRLFRWSLTQQ
                     SLFIESLLLDMPIPPIYVDEQEDGRYVLVDGLQRISSYLNFRGITLDSPELTAIDPDD
                     DDCEIDFERVEPGFQLSGCEIIDDLNGKEFDELSIEEKRQLKRTFIRVEVLTKENPKE
                     IKYHMFKRLNSGGALLSKQELRNSNIRMIDEKFINFINELANSEDFVELTHYMTESDK
                     QQMKRAEVVLRFFLFKNKFINDNPDVRMGFLDDELTTYLEEVSSQKFPFEYETEKENF
                     LSLVSYLKNHFGSVIFSGISKNNTISKRFVQYNYDGFMQYFSKESNRDSSITIDKINE
                     IKNSDEYKQYRTGGLENSRKRVEVIAGKVNE"
     gene            complement(1747..4398)
                     /locus_tag="VWZ30_000383"
     CDS             complement(1747..4398)
                     /locus_tag="VWZ30_000383"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU9334188.1"
                     /translation="MSKELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPELGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(4391..4969)
                     /locus_tag="VWZ30_000384"
     CDS             complement(4391..4969)
                     /locus_tag="VWZ30_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9334189.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYQLMYLKQIQDLLDNFHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQHQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(4969..5529)
                     /locus_tag="VWZ30_000385"
     CDS             complement(4969..5529)
                     /locus_tag="VWZ30_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9334190.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAACALIDLAFKELGLHKIELTCFGYNLQSQRVSEKLGFTLEARI
                     RDRKDAQGNCCDDLRYGLLRSEWEVI"
     gene            complement(5516..5965)
                     /locus_tag="VWZ30_000386"
     CDS             complement(5516..5965)
                     /locus_tag="VWZ30_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEU9334191.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVVPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(6378..6773)
                     /locus_tag="VWZ30_000387"
     CDS             complement(6378..6773)
                     /locus_tag="VWZ30_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9334192.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(6869..7123)
                     /locus_tag="VWZ30_000388"
     CDS             complement(6869..7123)
                     /locus_tag="VWZ30_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEU9334193.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            complement(7133..8467)
                     /locus_tag="VWZ30_000389"
     CDS             complement(7133..8467)
                     /locus_tag="VWZ30_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU9334194.1"
                     /translation="MTDERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(8467..8700)
                     /locus_tag="VWZ30_000390"
     CDS             complement(8467..8700)
                     /locus_tag="VWZ30_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU9334195.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            complement(9115..9297)
                     /locus_tag="VWZ30_000391"
     CDS             complement(9115..9297)
                     /locus_tag="VWZ30_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334196.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(9299..10082)
                     /locus_tag="VWZ30_000392"
                     /pseudo
     CDS             complement(9299..10082)
                     /locus_tag="VWZ30_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10361..10711)
                     /gene="rbfA"
                     /locus_tag="VWZ30_000393"
     CDS             complement(10361..10711)
                     /gene="rbfA"
                     /locus_tag="VWZ30_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU9334197.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            complement(10962..13754)
                     /gene="infB"
                     /locus_tag="VWZ30_000394"
     CDS             complement(10962..13754)
                     /gene="infB"
                     /locus_tag="VWZ30_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU9334198.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKSAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQVAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            complement(13771..14070)
                     /locus_tag="VWZ30_000395"
     CDS             complement(13771..14070)
                     /locus_tag="VWZ30_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEU9334199.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            complement(14063..14356)
                     /locus_tag="VWZ30_000396"
     CDS             complement(14063..14356)
                     /locus_tag="VWZ30_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU9334200.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            complement(14378..15514)
                     /gene="nusA"
                     /locus_tag="VWZ30_000397"
     CDS             complement(14378..15514)
                     /gene="nusA"
                     /locus_tag="VWZ30_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU9334201.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            complement(15558..16037)
                     /gene="rimP"
                     /locus_tag="VWZ30_000398"
     CDS             complement(15558..16037)
                     /gene="rimP"
                     /locus_tag="VWZ30_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU9334202.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            complement(16163..16798)
                     /gene="trmB"
                     /locus_tag="VWZ30_000399"
     CDS             complement(16163..16798)
                     /gene="trmB"
                     /locus_tag="VWZ30_000399"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU9334203.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            complement(16795..17589)
                     /locus_tag="VWZ30_000400"
     CDS             complement(16795..17589)
                     /locus_tag="VWZ30_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU9334204.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            complement(17751..18362)
                     /locus_tag="VWZ30_000401"
     CDS             complement(17751..18362)
                     /locus_tag="VWZ30_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9334205.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGDFFQNESALIIVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYNSWPLLYYL"
     gene            complement(18392..18640)
                     /locus_tag="VWZ30_000402"
     CDS             complement(18392..18640)
                     /locus_tag="VWZ30_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334206.1"
                     /translation="MYKHLFFLDSKTLDRLTPYIIVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLKWFVLERLDLKNDV"
     gene            complement(18683..19372)
                     /locus_tag="VWZ30_000403"
     CDS             complement(18683..19372)
                     /locus_tag="VWZ30_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9334207.1"
                     /translation="MKKYQLVFKISAVLSYLFFVFGLSQLTLIVQNHWQFSSQVGNFF
                     WIRNILSLLFSGVMIWILVKTGHGYLFRIPRKKWLWYSILTVLVVVLHISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYVTLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFLSLPSLLDIFVFATFGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            complement(19404..19637)
                     /locus_tag="VWZ30_000404"
     CDS             complement(19404..19637)
                     /locus_tag="VWZ30_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComC/BlpC family leader-containing
                     pheromone/bacteriocin"
                     /protein_id="HEU9334208.1"
                     /translation="MDTKMMSQFAVMDNEMLARIEGGDVSDIYRGYANQVSPFGSYPP
                     ILKNSGPFPVSGYCPRGYHDRGYIGAGFHLCGI"
     gene            complement(19883..20341)
                     /locus_tag="VWZ30_000405"
     CDS             complement(19883..20341)
                     /locus_tag="VWZ30_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334209.1"
                     /translation="MKSSEETVTESVRDENTRSDSMNISDKVVSDSKGMSDKKRTEMK
                     ISNANSENSGKVESTNSTSEAKTTQSGNLAMLDNKNQLETLKPIFDAAQENKTSLTDV
                     KDADYVSESITSNEKLLLKTGEEESYQLIRLAVAFFMASIMIKVKQKIHK"
     gene            complement(20484..20909)
                     /locus_tag="VWZ30_000406"
     CDS             complement(20484..20909)
                     /locus_tag="VWZ30_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9334210.1"
                     /translation="MKKQKIKSLLLVPLVFASITAGLAYAEEQPTTSSTQANDSSLVA
                     PDLPSAKEKVEHKPSVVTPEEYEKNVTDFKKIDIEAVRQSFTGDQLEHTIYFGRKTCS
                     HCRQFSPELKEFNNLIEKKLEYYDLDGKDFDGEAREFLF"
     gene            complement(21070..21474)
                     /locus_tag="VWZ30_000407"
     CDS             complement(21070..21474)
                     /locus_tag="VWZ30_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU9334211.1"
                     /translation="MKIFLAKKRNIFLARLFLGQLPLLVSTYLFLSRQFLNFSLVFQF
                     FLVVINLASILVTVYLTREMRIKEFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITA
                     GESYQQIIAYIGAILCLTIMLLLIWGLKYYKK"
     gene            complement(21471..21635)
                     /locus_tag="VWZ30_000408"
     CDS             complement(21471..21635)
                     /locus_tag="VWZ30_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334212.1"
                     /translation="MKKKILIIFVLYLIMSIFLYPLRESAWYQLFYTIAYVIAVMIYF
                     AINKKKGEKK"
     gene            complement(21933..22052)
                     /locus_tag="VWZ30_000409"
     CDS             complement(21933..22052)
                     /locus_tag="VWZ30_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEU9334213.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSPKKDTDSTP"
     gene            complement(22156..22305)
                     /gene="blpO"
                     /locus_tag="VWZ30_000410"
     CDS             complement(22156..22305)
                     /gene="blpO"
                     /locus_tag="VWZ30_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEU9334214.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CVTTV"
     gene            complement(22549..22743)
                     /gene="blpN"
                     /locus_tag="VWZ30_000411"
     CDS             complement(22549..22743)
                     /gene="blpN"
                     /locus_tag="VWZ30_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEU9334215.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGKILGP
                     WGAAIGGAVVCGYLAYTATS"
     gene            complement(22759..23013)
                     /gene="blpM"
                     /locus_tag="VWZ30_000412"
     CDS             complement(22759..23013)
                     /gene="blpM"
                     /locus_tag="VWZ30_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEU9334216.1"
                     /translation="MNTKMMEQFEIMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVALWAGVTGATGGF"
     gene            23294..25447
                     /gene="blpA"
                     /locus_tag="VWZ30_000413"
     CDS             23294..25447
                     /gene="blpA"
                     /locus_tag="VWZ30_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter BlpA"
                     /protein_id="HEU9334217.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFSEWTGVAIFLAPKPSYQPHKDKKNGLLSFLPL
                     IFKQKSLIAYIVLSSLLVTIINIGGSYYLQGILDEYILNQMKSTLGIISVGLVITYIL
                     QQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANSII
                     DALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFEKMNH
                     DVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYSILQT
                     SLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQTKLQS
                     AKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDINLTIK
                     QGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINYLPQQ
                     AYIFNGSILENLTLGGNHMISQEDILKACELAEIRQDIERMPMDYQTQLSDGAGLSGG
                     QKQRIALARALLTKAPVLILDEATSGLDVFTEKKVIDNLISLTDKTILFVAHRLSIAE
                     RTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            25458..26819
                     /locus_tag="VWZ30_000414"
     CDS             25458..26819
                     /locus_tag="VWZ30_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEU9334218.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSFVATKE
                     ITVTSQGEIAPTSAIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFRNFTKQSHDIELGISKTN
                     TEVSNQANLANSSSSAIEQEITKVQQQIGEYQELRDAIINKRARLPTGNPHQSILNRY
                     LVAAQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIIHLSSEFEGKNRI
                     PTGTEIAQIFPIITDTREVLITYYVASDYLPLLDKGQTVRLKLEKIGNHCITIIAQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTAKKTYFDYFKDKILT
                     HSD"
     gene            26876..27031
                     /gene="blpC"
                     /locus_tag="VWZ30_000415"
     CDS             26876..27031
                     /gene="blpC"
                     /locus_tag="VWZ30_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEU9334219.1"
                     /translation="MDKNQNLTSFQELTTTELNQITGGGWWKDLLHRFNVIEQNNTKG
                     FNQPIQL"
     gene            complement(27073..28413)
                     /locus_tag="VWZ30_000416"
     CDS             complement(27073..28413)
                     /locus_tag="VWZ30_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9334220.1"
                     /translation="MNIVWILLHTLITNGLEIVIFFKVDGINLTFERIFKAFLLKILL
                     VFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRREILDTGFQK
                     SLTKINWIMGAYYLVIQSLSYFEYEQGIQSTTVRHLILVFYLLFFMGVIKKLDTYLKN
                     KLHERLNQEQTLRYRDMERHSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYDSVLKDSSQKLQDNKYDLGRLVNIRDRALKSLLAGKFLKARDKKIIFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASAEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGVSSKGEERGVGLYTVMKIVESYPNASLNTTCQDQVFRQVLTMIHTE"
     gene            complement(28427..29165)
                     /locus_tag="VWZ30_000417"
                     /pseudo
     CDS             complement(28427..29165)
                     /locus_tag="VWZ30_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(29170..29508)
                     /locus_tag="VWZ30_000418"
     CDS             complement(29170..29508)
                     /locus_tag="VWZ30_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9334221.1"
                     /translation="MKHMIIQTQKTVYKVNVDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQYGETLMRCHRNCLVNLDKLKSIDFQKRILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            29764..30093
                     /locus_tag="VWZ30_000419"
     CDS             29764..30093
                     /locus_tag="VWZ30_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9334222.1"
                     /translation="MTDTDPIKRAQALITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETADNRVLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLSL
                     YGPTIIL"
     gene            complement(30154..31203)
                     /locus_tag="VWZ30_000420"
     CDS             complement(30154..31203)
                     /locus_tag="VWZ30_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9334223.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWIFVQTLFLLLFAPLFLAMGYGLPVFLVYVLLLGVGKYFLFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKVVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSLLAQVFIEQSWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQAVVRGLTSLVLVVELVVGLVTFQEKLALLALLGAGLVL
                     QVLYLPYQVKRQMQD"
     gene            complement(31200..31931)
                     /locus_tag="VWZ30_000421"
     CDS             complement(31200..31931)
                     /locus_tag="VWZ30_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9334224.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYKELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            31999..32409
                     /locus_tag="VWZ30_000422"
     CDS             31999..32409
                     /locus_tag="VWZ30_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9334225.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            32419..32706
                     /locus_tag="VWZ30_000423"
     CDS             32419..32706
                     /locus_tag="VWZ30_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334226.1"
                     /translation="MKLSNLLLFAGATAGSYLVTKNRQTITDEILNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKILEKDIQTRLAVIKEMQGTEDK"
     gene            32867..33106
                     /locus_tag="VWZ30_000424"
     CDS             32867..33106
                     /locus_tag="VWZ30_000424"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334227.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDKVSSKHCF
                     EVVDRTDEVSNHTHGKPTLTWFEEIFEEYHSPFHN"
     gene            complement(33432..34568)
                     /gene="dnaJ"
                     /locus_tag="VWZ30_000425"
     CDS             complement(33432..34568)
                     /gene="dnaJ"
                     /locus_tag="VWZ30_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU9334228.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(34912..35316)
                     /locus_tag="VWZ30_000426"
     CDS             complement(34912..35316)
                     /locus_tag="VWZ30_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEU9334229.1"
                     /translation="MDEEYLKNTRKVYNDFCNQADNYRTSKDFIDNIPIEYLARYREI
                     ILAEHDSCIKNDEAVRNFVTSVLLSAFVSALVSAMISLEIQTYKFVIPFIIGVIWTVV
                     VFLMINWNYIANTKKRQKYINISVLIGYLKSK"
     gene            complement(35327..37150)
                     /gene="dnaK"
                     /locus_tag="VWZ30_000427"
     CDS             complement(35327..37150)
                     /gene="dnaK"
                     /locus_tag="VWZ30_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU9334230.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNSDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVIAGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKTKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQETGNAGDDVVDGEFTEK"
     gene            complement(37630..38154)
                     /gene="grpE"
                     /locus_tag="VWZ30_000428"
     CDS             complement(37630..38154)
                     /gene="grpE"
                     /locus_tag="VWZ30_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU9334231.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(38181..39215)
                     /gene="hrcA"
                     /locus_tag="VWZ30_000429"
     CDS             complement(38181..39215)
                     /gene="hrcA"
                     /locus_tag="VWZ30_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU9334232.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKAIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVVAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYL
                     YLSSNHYEVH"
     gene            complement(39379..39888)
                     /locus_tag="VWZ30_000430"
     CDS             complement(39379..39888)
                     /locus_tag="VWZ30_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEU9334233.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSCFGLFYFEQ
                     GQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLGGLVVCMSSFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            40496..42829
                     /locus_tag="VWZ30_000431"
     CDS             40496..42829
                     /locus_tag="VWZ30_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9334234.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKSGEHITYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKEDSNWRKVIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIGD
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAMVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPVKVLE
                     TGAKTVSGSTPGFVGEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLDKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            42842..44305
                     /locus_tag="VWZ30_000432"
     CDS             42842..44305
                     /locus_tag="VWZ30_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9334235.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERAALNHKIDNVLADILQLLEDK"
     gene            44305..>44494
                     /locus_tag="VWZ30_000433"
     CDS             44305..>44494
                     /locus_tag="VWZ30_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HEU9334236.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDD"
BASE COUNT        13455 a         9502 c         8066 g        13471 t
ORIGIN      
        1 aatcctttct gtttccatga gcaatcatat ttcgataagt tattattgaa tcaatgataa
       61 ttgaatagga tttatatttc tttttatcaa aatgaaatat ttcaagaatt tctgtaaaat
      121 tattgaaatt cagatttgat ttcgtatcaa tcatattttc attgctattg aattcggttt
      181 gttgtttaat tttgatatgc aagtccgtaa gtggtttaat aactgtgtta tcttctctct
      241 tctcaattat ttttttgcgt aattcatcta gcttttgtcg aaaagttagc atttgtagtt
      301 catctgtcaa tgaatctaga ctatttttat cgattgttag taaggcagta ttacagcatt
      361 ccttgatggt atactcaaag gtagcataga ctgaaaatat caatttatat aatgctccat
      421 tatccttaaa gattgctagt tcttttagaa tattaccctc aggagagtcg gataatacta
      481 aatttaaaaa attctgttca tctttaatac tatcgattga aactgctaaa attttgtcaa
      541 gagaatccct aatatcactc atttactttc cctgctatta cctcaacgcg ttttctagaa
      601 ttttcaagcc ccccagttcg gtattgctta tactcatcac tattttttat ctcattaatc
      661 ttatcaatag taattgatga atctctattg ctctcttttg agaaatattg cataaaaccg
      721 tcgtagttat actggacaaa tcttttagaa atagtattgt ttttgctgat accactgaaa
      781 ataacactac caaaatgatt tttaagatac gaaactaaac tcaaaaagtt ttccttttct
      841 gtttcatatt caaaaggaaa cttctgtgag gaaacttctt ctaaataggt cgtaagttca
      901 tcatctaaaa atcccattct tacatcggga ttatcattaa taaatttatt tttaaacaag
      961 aagaatctta aaacaacttc agctctcttc atttgctgct tatcagattc tgtcatgtaa
     1021 tgtgtcaatt caacaaaatc ttcagagtta gccagttcat taataaagtt tatgaacttc
     1081 tcgtcaatca tgcgtatatt actgtttctt aattcttgtt tagatagcaa tgctccacca
     1141 gaatttaatc ttttaaacat atgatattta atttcttttg gattttcttt tgttaagact
     1201 tctactcgaa taaacgtacg ttttaattgc cgtttttcct ctatgcttaa ctcgtcaaat
     1261 tctttaccat tcaaatcatc aataatttca caacctgata attgaaaacc tggttcaact
     1321 cgctcaaagt ctatctcgca gtcatcatca tctggatcaa tagcagttaa ttctggagaa
     1381 tctaacgtta tgcctctgaa atttagataa gatgaaattc tttgtagtcc atctactaga
     1441 acatacctcc catcttcttg ctcatctaca taaataggag gtataggcat atctaaaaga
     1501 agggactcaa taaagagaga ttgctgtgtt aatgaccatc taaataatct ttggaattct
     1561 ggcttaataa ctaattcgcc atcattatac atatcataaa gttcattaaa cgatatatca
     1621 aaatttcttc tgacaagttt attttgtgcc ataaaaaagt ctcaactttc aataaatatt
     1681 tataatattg attataccat aaaaacacag tttttacact gtgttttatt aaatttatat
     1741 aattgtttat ttcaccaact tcttcatctc atcaatacgt gctacggtcg catcatactt
     1801 ggcttggtag tcagcttgtt tgtcacgttc tttttggacg acttctggtt tggcgttggc
     1861 tacgaagcgt tcgttagaga gtttcttacc aaccatgtcc agttcttttt gccatttagc
     1921 gagttctttg tcgagacgag ctagttcttc ttcaacattg aggagatcgg ccagtggcag
     1981 gtagatttct gctcctgtga tgacacttga catagccagt tcaggtgcag ggatggttga
     2041 tgcgatttcc aaatgttctg gatttgtaaa gcgtttgatg tagttgacat tgctgttaaa
     2101 gaaggcttcc aagtcactat cgctagtctt aacaaggatt gtgataggtt tgcttggtgc
     2161 tacatttact tccgcacgcg cattacgaac ggcacggatc aagtctttga gactttccac
     2221 accagtgtgg gcagcaaggt cttcaaaggc tagattaaca gttgggtatg cagctgtcac
     2281 gatagaacct tctgagattt gtccaaagat ttcctctgtc acgaatggca tgattgggtg
     2341 aaggagacga aggatcttgt ccagcgtata gaggagaaca gatcgagtaa tgaccttatc
     2401 gtcttcattg tcgctgtata gaacttcctt ggtcaactca acataccagt tggcaaattc
     2461 ttcccagatg aagttgtaaa ggatatgacc agccacacca aactcgaact tatcaaagtt
     2521 ttcagtaact tttgcaatgg tttcgttgag attgtggaga atccagcggt ccgtcacatt
     2581 accagcctca cctgttgcaa cttttgtgac attgtcatgc gccacatcca gcgtcaaacc
     2641 ttcattgttc atgaggatat agcgagaaat gttccaaatt ttgttaataa agttccatga
     2701 agcatccatt ttctcgtaag agaaacgaac gtcttgacct ggtgcggaac cgtttgaaag
     2761 gaaccaacga agggcatcag caccgtattt ctcgatgaca tccattgggt caatcccgtt
     2821 accgagagat ttagacatct tgcgtccttg ctcgtcacgg atgagaccgt ggataagcac
     2881 gttttggaat ggctgacgac cagtaaattc caaggactgg aagatcatac gagacaccca
     2941 gaagaagatg atgtcgtaac ctgttaccaa ggttgaagtt gggaaataac gtttaaagtc
     3001 ttctgagtcg acttcaggcc agcccatggt tgaaaatggc cagagggcag aactgaacca
     3061 agtatccaag acgtcttcgt cctgagtcca tccgtcacct tctggagctt cttcgccgac
     3121 atacatttca ccatcagcat tgtaccaggc agggatttgg tgaccccacc aaagctgacg
     3181 agagataacc cagtcgtgga cattttccat ccattgaagg aaggtatcgt tgaaacgagg
     3241 tgggtagaat tcgaccttgt cctctgtgtc ttggttggca atggcgttct tagccaattg
     3301 gtccatcttg acgaaccatt gagtagacaa gcgtggctca actacgacac ctgtacgttc
     3361 tgagtgacca acactgtgga cacgtttttc gattttgaca agggcaccga tttcttccaa
     3421 cttagcaacg actgccttac gagcttcaaa acgatccatg cctgaaaatt caaaggcaag
     3481 ctcattcata gttccgtcgt cgttcatgac gttgacttgt ggcaagttat gacgttggcc
     3541 aaccaagaag tcatttggat cgtgggcagg tgtgattttc acgacacctg taccaagctc
     3601 aggatctgcg tgctcatctc caacgattgg gatgagttta ttagcgattg gaaggatgac
     3661 gtttttacca atcaagtcct tgtagcgcgg gtcttctgga ttaaccgcaa ccgcaacgtc
     3721 cccaaacata gtctcaggac gagttgtagc aacttcaagg gcgcgtgaac catcttccag
     3781 catgtaattc atgtggtaga aggcaccttc tacatccttg tgaatcacct caatatcaga
     3841 aagggctgtg cgagctgctg ggtcccagtt gatgataaac tcaccacgat agatccagcc
     3901 tttcttgtaa agatccacaa agaccttacg aacagctttt gacaaacctt catcaagagt
     3961 gaaacgctca cgagaatagt ctacagaaag ccccatcttg ccccattgtt ccttgatggt
     4021 agtggcatat tcgtctttcc attcccagac cttcgtcaag aaagactcac gacctaggtc
     4081 ataacgcgta ataccctcac cacgtaagcg ctcctcaacc ttagcctgag tcgcaatacc
     4141 agcgtggtcc atacctggaa gccaaagggt atcaaagcct tgcatgcgtt tttgacggat
     4201 gatgatatcc tgcaaagtcg tatcccaagc gtgaccaagg tgaagtttcc cagttacgtt
     4261 tggtggtgga atcacgattg aataaggctt agccttttga tcgcctgaag gcttgaaaac
     4321 atccgcatca agccattttt ggtaacgacc agcctcaacc tcggctggat tgtatttagg
     4381 tgaaagttct ttagacatgt gtgtgtcctt tctctatttt gtttatttta ttttgaattt
     4441 gcttagcagc ttcttctgca gacaaattcg tattatttat tttaaagtag tggtgcaact
     4501 cattcggttg atgttgggaa tttaattgaa gtgtttcagc ggtctctaaa atttctcttt
     4561 cagatacctc aatatgtcgt tttaagggtt tgtgctttaa tcgattctcc gttcgatttc
     4621 gacgtatgca ctcttcaaga cttgtttcca attcaacaaa cagaatctct tgatgaaagt
     4681 tatccaataa atcctgaatt tgctttaaat acatcagctg gtactgattt gaaaaatcaa
     4741 ttacgtctgt taaaattact gatcgctgat ttcttgcact tgctccaagg aaagaaaagg
     4801 taattccacg aacaaattcc cacatctcct cggtataatc ctgatagatc tctagtgcaa
     4861 aatcaatggc ttgatggtta taaaataggg tagcatccgt cagtcgagat aattcttgac
     4921 caatggtcat ttttcctgat gctggagcac caatgatgaa aagatgcatc aaatcacctc
     4981 ccactcactc ctcagcaagc catatctcaa atcatcacag cagttgcctt gagcatcttt
     5041 gcggtctctt atgcgagctt cgagggtaaa gccaagcttt tccgagactc gttgactttg
     5101 aaggttatat ccaaagcaag ttagttcaat cttgtgaaga ccaagttctt taaaagctag
     5161 atcaatcaag gcacacgctg cttctggaac ataacctcgc ccccaatagt ctgggtgtaa
     5221 ggtatagccg atttccagca catcatcttc atggcgatgg ttgaaatcga cagagccaac
     5281 gatcttatcc gttcctttga cgacaatccc gtagcctgct gggagatttt ccttttgatt
     5341 acgctccgga agaatgtgct ccagataata aatctcatct tccaaggtct tgactggagg
     5401 aaaacctgct ggataggcaa cctctggcaa actagcatag tcaaagatat cctcagcatc
     5461 cgccacagta cggactcgta aaacgagacg ttctgtttcg attttatctg gcagctcagt
     5521 tcttgccatc cttcttcctc gcttttttga tgaaactgcc cttcatatct acacgcttgt
     5581 ccagatagcg ataaacgcgc tgatatccat ctcccatgaa ataggttggg gcaaacagtt
     5641 gatttttaaa atgtcccttt tcatccagga gttctggaac aacaagtcgc tcaagaatct
     5701 tggcaaagat gtggcaaata ccgtcttcct caacaatcct atctacccga caatctaaaa
     5761 caagtggaca ggcgtctaaa ataggagtct gagttcgttc agaaatttca taatgcactc
     5821 ccaaacgttc caatttctcc tgatgactga taaaaccagc ctgctccatc gcaagcatag
     5881 aagtttcatc agaaatattc acagtaaatt tttgatactg tttgatctgc tctgcggcat
     5941 tctctctcgc aacgactcca atcacaaccc aatctcctag actataagag gaactacagg
     6001 tcgtgatgtt atagccaaaa ttctaatctt gatatcctaa aataaaaaca ggaaaaccat
     6061 aatatagttt acttgtgtta aaagattgct tcataacaac cccctttgac taagacgtaa
     6121 aagaaaagcc ctgccatcta catgacaggg acgaatgtgt ttatccgcgg taccacccaa
     6181 tttcgggcag ttgcccgcaa ctctcgtctt taaagcaaaa agacactttt atttcaattt
     6241 ttcatccatt agcaaccagt tttgacacat ttgtggactt ctcagcgccg ccactttctg
     6301 taaaatgtgg gattcaaaac acctctaatg gctctattgt agcaagattt tttcggaaat
     6361 gcaaggctca agaaaaatta cttgaacttg atgccatttt ctttcaattt cttgatggta
     6421 gctgggccga ttcctttcaa ggcaaggagt tctttttcag tccagttttt gaaatctgaa
     6481 gcagacttga ttccttcatc atagaaagtc ttggcacgat caagtggaag tccacccaaa
     6541 tttgctgcaa aatcttctac agaattagct actttttgag cttgctcttt ggtagcttct
     6601 actgcttgtt caactttttt agaaacagcc ttaccagctg cgcttgctga ctgttctgca
     6661 cgcttcacga ctttttttgt ctcatctaca accttagtca cagtacttga aaaggcacct
     6721 gcgcgacgga ggccatttcg taattgtttt ttacgattga gtttctttga catgataaaa
     6781 tcctttcaat aaaaaatccc tgctctgaca aggatttaac ataaacattc tatcaagatt
     6841 ttagtgaaaa atcaagaatc tttctcgttt aataatttcg ctcaccaaac aagccttctg
     6901 gcaaatcatg gaatcccttg cctgctttga agttttcaaa cttaccaagc aagaactgat
     6961 aagcatccaa gcgactctcg tagcgaatca tggcatcttt ggcacgctcg tcctggtctt
     7021 cttcgtaaac tttttgactt tccttgtgcg ccatatcgtt aatcatgatt tgggtattga
     7081 cccaagcttc aaattccttc ataaattctt gttcgtaact cattttttct ccttattcta
     7141 atccacgaaa atagtccgta tcaatctcac ggtagggctg gatatcctga acatactcca
     7201 acactccttg gaattctccg tcttcatcgt gtactgcagc ataggtgatg tggacaaact
     7261 tacctcgcga ctcagacttg aaccacattt catacttgtc tttgcttccc tcacgaagcc
     7321 ccttcatgat agttttgacc ttgtccaagt acttaggcgg atggcagagt tcgacattgc
     7381 gcccgacttg ggacggcgtc cgtttgaaaa tcatctcatc agctggcgta ttgtcattgt
     7441 aatactggaa aatatcttct ttattgacaa aggtaatctc catagggaga tgattgagga
     7501 tgagattggc ctgctcgact gaaagatagc cattaccaaa agcctgttga ctatggcggt
     7561 ccagcacagc ttccttttcc ttaggggtaa aggtaatggt aaaatggcct tctggcgtat
     7621 ctatgacttg ttgaacttga ccttctgccg tatctagctg tacaggttcc tctgcaatct
     7681 tttcctcaat aaagctctgt cgttctggca cccatttctc tgacggacgg atgatggcat
     7741 agccataggc atcgctctcc tccgcaatct gaagccagtc atcctgagta aaagactcaa
     7801 ggagaatcat gaggaggatg gactcttcct tgaaaatcat actttcaaac tctgtcgcaa
     7861 aagcttcaaa agcttccttt acactgctaa ttgacacttc tggtagtgac ttggctgtcg
     7921 ttagagctgt ttgaaagagt tccctaatct gatcatccac tccccacata actttgggag
     7981 gtgaatcgtg tccatagcgc tccatgatag gaaagaagag ttcttcctta cgttggtaat
     8041 ggatgtcaaa ttgccccaca agccccatct gacgcaccaa gcccttgcgc atctccgcaa
     8101 gcatttcctc gtcttccata gactcatagg tatctaacaa tctacgaatg cgaatcaagg
     8161 ccgcacggag agccagattt tcttctttga agacacgaac tgggtgacct ggatgctcag
     8221 tatctgaaac ttcgacacct ttgatagcat ttttaaaaag attggcatgg acatcacaga
     8281 gttccataac atcttcaaag gtgacacctg agtctgagtt catcagctcg tgctccataa
     8341 gggaaatctc aatggctgag acacccgcaa aggtcgcatc aaaacgctcc tgaaccgact
     8401 caggagaggc gccattatgc aattctaata aaatatcccg taggacatga atccgttcat
     8461 ctgtcattag tctaatccaa tcacttcgta gccattcgct tccagtgtgc gtacaatctt
     8521 gtccatagga gttcctgcta gcttagaacc ctgtttaagt gatactttac gaccaactgt
     8581 attgcgcatt aagggattgg caaggggttt aaaacccagc tccactagaa tttccaagac
     8641 ttctggatgc ttgtccacca cttctgcaac aggaattgac acatcgatga tattgtccat
     8701 gacgacctcc attatatgtt ctaatactca atgaaaatca aagagcaaac taggaagcta
     8761 gccgcaggtt gctcaaaaca ccgttttgag gttgtggata gaactgacga agtcagtaac
     8821 atatatactg caaggtgaag ctgacgtggt ttgaagagat tttcgaagag tataaaatga
     8881 ttttactgct tatattatac catatggaga gagattaaaa aactagcagt ggagttcctg
     8941 ctagctctct gcgacttgga ttaccatcta gataagttta aagagccaat ccaatatctt
     9001 aaataatatc ttgtaaaaca gcaattgaac tgcaaaagaa atgatcatcc aaaagaattt
     9061 cacaatccca ataatcggtt tgataaaaag aatagcaaga agaccaaaaa aatttcattc
     9121 tctctcctct ggcttaatga gccaccgagc cgtatccatt agcttatctg caataaaaag
     9181 tttgcccaga ccaacaacga catggtcagc tttctttatc gttttcatca cttttacacc
     9241 ttgcacggtc aaaaagtaat taccataaat tttagcaaaa gctttgatag gattcatatt
     9301 actctccttt gatgatttca gcctcttttc ggattgtttc aacatcttct tgatattgaa
     9361 cttcactata gttgactagc ttggataatt ctttctgcaa gctttccccc attggtttca
     9421 tagttgcaaa ggttaaacca cctgaaatga taccacccaa gataggaata aatttcccta
     9481 ttcctttggc cagccctccc ttggtaagat tcacaccaaa tattcttaag acttttttca
     9541 aaatagggta ccaaagcgtc tttgttaaag ctttattagg cactgttttc attacctgtt
     9601 tggcaattgt tataccacca gcacgtagca aacaaagcag cggttccatt cacccctaac
     9661 attacgccta gataaagcaa gagggtattt tgagtatctt cactcaactc ctcacgtgaa
     9721 gcccaaagat cctcataacc ataaatataa cctaattctt gggccaattt cagagagaaa
     9781 ccataaaatt gagccacata agctggaatg gtaattgcca tagcaagccc tccaggtaat
     9841 cctgccaaaa cagaagtccc actcgctaat aaaacattat ccctaataca ggcattagcc
     9901 actcgatcta agatttcttg agataagaga gacgttggac cttgttccaa taaggtagga
     9961 atgtcctgtg gacccaattc tttggaaaac ttatccacta aaaatttcga acgatcaacc
    10021 ttaacaactg gtagttttac cacttgttgt aatacttgca tagctaagtc ttgttcagcc
    10081 atatacaaac tccttatttc ttttttgcac cttatcatat tatatttcct acaaaaaaag
    10141 cattttctaa ttagataaca ttaaaaaata cagaacccct tttttatagt aaaataaaac
    10201 aagaacagga caaatcgatc aggacagtca aatcgatttc taacaatgtt ttagaagtag
    10261 aggcgtacta ttctagtttc aatatatttt aaaagaatat ttttccattt caaattcaac
    10321 ctattttcct ccaccaaaaa agaggggcaa cccctcttct ttagttctta tccagattgc
    10381 gtagcatctc gtcaatcttg tttccatact cgatggactc gtctttgacg aaggtcaaat
    10441 ctgggatttt gtacaatttc aaattgcgac caagttcacg tttgatggta ccagttgctt
    10501 tttcaagccc gatttgggct ttttggttat ccgaagcaag gttactcaaa atggtgtaat
    10561 aaaccttggc aacagacaag tcacccagca tctgaacatc tgtgatggtc acaccttgga
    10621 cacgtggatc acggactttc ttttgcaaaa tctcattgac ttcacgcttg atttccatgc
    10681 ccacacgatc tgtacggaaa tgatttgcca tgatattcct ttctctactt tgaaccaaaa
    10741 gctaggccag cagacctagc tattttaaac ttcttgattt tcagtttaaa ttgaaacgaa
    10801 gttcaatttg aagtcatctg cttctttcgc cgtggtgaaa gtgatggaac tgatttatca
    10861 gtcccatcac aggggatttt tgagacccta ggctcaaaaa taagcaatga aaccatcggt
    10921 ttgcttgcgt ccctcaccac ctaagaaagg agcaaaaaat cttatctctt gatttcttcc
    10981 atgacatacg cctcaatcac atcatccatc ttaatatcat tgtagccgtc gatcatcaat
    11041 ccaccttcac ggccgtttgt aacttcctta acgtcgtctt tatagtgttt caagcttgcg
    11101 agttcaccat catagataac gacaccatca cggataacac ggactttaga gtcacgggca
    11161 accttaccgt tgataaccat aaatccaccg atagttccca ctttagacac cttgaaggtt
    11221 tcacggataa ccgcttcacc aataactttt tcttcaaatt ctggatcaag catccctttc
    11281 atagcttctt ccatctcttc gataaccttg tagataatgc tgtgaagacg gatttccaca
    11341 tcgtcagctt ctgcttgttg acgagcttgt ggtgtagggc gtacgttgaa accaacgata
    11401 aaggcatttg aagcttcggc aagggtcacg tctgattcgt tgatagcacc gaccgctgag
    11461 tggacgatag tcactttgac accttccacg tcaatctttt gaagtgaggc agaaagtgct
    11521 tcaacagaac cttgtacatc ggccttgatg ataacattaa cagatttgag ttccccagct
    11581 ttaagggtat caaagaggtt ttcaaggcta acacgttggg tagcttgacg ttgtttcatg
    11641 agggcacgtt tggcacgctc ttcacctgct gcacgcgcag atttttcatc ctcgtaaacg
    11701 gcaaagtggt cacccgccat cggtgcttcg ttcaaacctg tgatagagac tggtgttgat
    11761 ggtccagcaa ctttaacacg acgaccaagg tcgttggtca tagcacggac acgaccgaag
    11821 gtatttccga caacgattgg gtcttgaaca ttcaaggtac cttgttgtac aagaagggtt
    11881 gcgaccgcac cttttccttt atccaagcgc gcttcgataa ccgtaccgat tgcacgcact
    11941 gttgggtctg ccttgagttc ttggatttca gctacaagaa ggactgtttc caacaattct
    12001 tcgatatttt ggttgaattt agctgagatt tcaacaaatt cagaatctcc accccaagca
    12061 gttgacataa ctccatgctc tgccaattca ccgataacgc gttctgggtt agcacctggt
    12121 ttatcaatct tgttaatggc tacgatgatt ggaacgttgg ccgcttttga gtggttaatg
    12181 gcttcgatag tctgaggcat aaccccgtca tctgccgcta cgaccaagat cgtaatatcg
    12241 gtaacagaag caccacgcgc acgcattgat gtaaaggccg cgtgtcctgg tgtatcaagg
    12301 aaggtaatct tcttaccatt ttccacgatt tggtaggcac cgatatgctg agtaatacca
    12361 cctgcttcac ctgtcgcaac acgtgagtta cgaagagtat ccaaaagggt tgttttaccg
    12421 tggtcaacgt gtcccatgat agtaacaact ggtggacgct caaccaattc atcttcattg
    12481 agataaccat cttcgacaaa gaaacgttcg atgtcagcat tatccacttc aaccttttgt
    12541 ttagcttcga taccgtaatc caccatgagg agttcaattg tttccccatc caaggattgg
    12601 ttttgtgtgg ccatgacacc catcatgaaa agtttcttaa cgatttcagc tggttcacgt
    12661 ttgatacgtt ttgcgatttc cgcaacggtc ataccatctg tatattcaaa ttctgttggc
    12721 aattcatgga atttacgctc cgtaacaggt tttggagtct gattacggtt gttcttgtta
    12781 ttgccttttt tgttcttttt gttgttattc cagttactat tcttttgatt tctcacttga
    12841 ttttgactac ttcgattctt ttgttgtttt ctaggaccat cttcttcatg atcataatcg
    12901 tcacgatttt tgtctggtcg agcttgtttt ttacgacgtg tatccactgc aggttctttt
    12961 ttctcaggga cgacttcaac cactgcttga acttgctgtg cttgttcagc taacttagcc
    13021 gcttcttcaa agatttcctc tggttccttg cgtttgttag cttgagccaa ggcttcttta
    13081 gcagcctgat actgcttgaa gcgttcctca cttgaacgag cgtactctgc attttgctct
    13141 gcttttaggg ctgctgcacg ggctttaaag tcaatacgtg gagccacttg atttgaacgt
    13201 ttgtcctctt gctgacggcg ctcatttcta cgtttttgct gaccttgctg cttcttagct
    13261 tggtcattaa agcgacgatt gtcgcggttg ctttgacctt gttttccacc attacggccg
    13321 tcgtttttct gacggtttcc gttttgttgt tggtcacggt tattgccctt attttgcttg
    13381 cgtcgttctg cctgctcttt agcacgggct tcacgctcag ccttgaaatt acgactttgc
    13441 ggtttcgctg ctactttttc tgttttcgga gcaactggct cagccggttt tgcctcttcc
    13501 ttagctacag ctggtttagc tggctcagat ttttcggctt tcttttctgc acttactttt
    13561 ggtgctgcag gttttgcttc tactttcgga gcagctgcag acttaaagct ggcagcaatt
    13621 tttgcagcga cagcttcttc cacacttgat gagtggcttt tcacatccaa gcccaactct
    13681 tttgcacgcg ctacaacttc tttactttct tttccaagtt cttttgcgat ttcgtacaat
    13741 cttttcttag acaaatcatg tcctcctctt ctattccata agagacctca ttttctttgt
    13801 aaatccagca tctgttacag ccaaaacctt tctcgatttc ccgactgcta tgcttaattc
    13861 cagtgttgaa aacacggtta caatttctac ttgataataa tgacttttat cttgaatctt
    13921 cttggtcaga ttgggtccag catcatgagc tagaaagacc aacttggcct tgccgtcttg
    13981 aatggccttg accaccaatt cttcacccga tatgatgcgc cctgctcgct gagcaagccc
    14041 caagagatta cttatctttt gcttattcaa gtcccaactc tcttcttttc actttgtgat
    14101 ccacataagc gatcaactcg tcataaaagc tttcttccac ttccatgcta aagctgcggt
    14161 taaagacctt cttctttttc gcctctaggg cttctgcatt gtctagtttg atataagcgc
    14221 cgcggccatt ggccttgccc gtaggatcaa taaagacttg tccttccttg ttcttgacaa
    14281 tgcggagcaa atcacgctta tcaatcactt cgttagacac aacagacttg cgcaaaggga
    14341 tttttcttgt tttcatcttt ccctcctcta gcagctttta ttcttctaca gtatcgtttt
    14401 ctacttccaa ctctactgaa gcagcgtctt ccatggcttc aaattcgcta gcagacttga
    14461 tatcgatacg gtaaccagtc aagtgagccg ccaagcgcac gttttgtcca cgacgaccaa
    14521 tggcaagaga aagcttgtta tctggaacaa ccaccaaggc acgtttgctg tcgttttcat
    14581 caaagataac ttggtcaacc tcagcaggag cgatggcatt gtagataaat tcagctggat
    14641 ctgctaccca ctcgataaca tcgatatttt cttcgattgg taccatgcgg tcatttttag
    14701 catcgtaacg agctgggtgg aatttgctag taatcttctt gatattagca ccaccacgtc
    14761 caacgattgt accgatagca tccacgtttg gattgtggct acgaacagca accttcgtac
    14821 ggtcacctgc ttcacgagcc acgctcatga tttcaacagt tccatcataa acttctggaa
    14881 tttcttgctc catcaaacgt ttgatcattt ctggatggct acggctaaca aagacgttca
    14941 caccacgagg gttgtcttca accttgtaaa cataaacttc gatacgatca tgagaagcaa
    15001 aaacttctcc aggaatttgg tcttgttttg acaattgggc ttcgatgcta ccaaggttga
    15061 catagataaa gcggttgtca aagcgttcta ctgtaccaga catgatttct tgctcatgtt
    15121 ctttgtaagt attgtaagtg atggcacgtg tttgcttgcg cattttttcc atgatggttt
    15181 gtttggcaga ttgggctgct acacgaccaa actcagctgg tgcttcttca aacttgattt
    15241 tgtctccaag ttcataagct gaattaatgg caagagcatc tttcaagctg atttccaaac
    15301 ggctatcaaa tacttcatca acaacttcac ggacagtata aactgtaaag tcacctgttt
    15361 tttcgttgaa gtcaatagct acgctgtctg actgaccata gcgtctgcga taagcggaac
    15421 gaagcgactc tactactgcg tcgatgatat cttctttttt gattcccttg tcttcttcca
    15481 aaatgcggaa ggcctctagc atttctttac tcatgttttc ttcacttttg aatcctcaaa
    15541 agctatcctt tctttttcta taatttaact gctaaacgtg cttttgatac taaactgtat
    15601 ggaatttgga cggttttctt acgcgtcttg tccatatatt ccatagtcaa ctcgtcctct
    15661 tcgaaggcca acaaggttcc ttcaaagacc ttttgcttat cgatggcttg gtagagcccg
    15721 acatggatgt attttccaac cgctccagcg acggcatcct tggttttcaa aggacgttcc
    15781 aaacctggac tggtaatttc taggaaatat tgttctggga agggatctgg cttgatggtg
    15841 tctaggacag gactgatcat ttctgtcaag tctgccgtgt cgttcaaggt aattccttcg
    15901 ggtttatcta caaaaatact gagaatcatg tcactgccaa tctttccata ctcgatatcc
    15961 acgagttcaa aaggagcttc tatgacaggt tctacaactt ctctgactaa ttctacgatt
    16021 gttgcgattg cgtccacctc ctcataagca agaggcgaag atatttcccc gcctcacttt
    16081 tcatattctt actatcagta tagcacgttt gccaacttat gtaaagcaaa agcacttgta
    16141 cagcctaatt ttaggctatc tcttaaaatt ctgcctcaac tcggtagata acttgcccct
    16201 tgtttgagaa tttttgctcg tattctgtca tgacattgcc ttcaaaatca ctggcatgca
    16261 aatctaacca gacaccattg agtttcatgc catattgaga aaagctcact aaactgtact
    16321 caaacaagcc acggttatcc gtcttgaaat gaatttctcc attttcaggc aagatacgtt
    16381 tgaaggtatc caagaaggtc ttgtaggtca aacgacgctt ttcatggcgt tttttcggcc
    16441 atggatctga aaagttcaga tacaagcgat caatctcacc gtcttcaaag tagtcagtta
    16501 agtcagaacc atctacccac aagagcttga tgttaggcac tccaacttca agcaccttgt
    16561 ccaaagcgta gctcaaaaca gacttttgaa tatcaatccc gatatagttg atgtcagggt
    16621 tttgcttggc catacctgaa acaaaggcac cctttccact tccaacttcc acatgaatgg
    16681 gattatcatt gccaaacaag tcccgccatt ttgccttggc ttccaaggga ttgaggacca
    16741 catactgggg atttgcctct agtaattctg ttgccccttt acgatttcta actctcatct
    16801 tctctttcca tacttgtctc ggaaatgacg caaaccatga atctcccgat tgacattttc
    16861 taaatcttgg ttcatataat acttggaaat ctgactcaaa taagacaatt gaccatacca
    16921 atacaattta cttaataccg tttgattgta cttgtaaccg tagtaggtca accattcctt
    16981 ccactgatgt tctgaaatat aatggcagag catatgggcc acatcaaaca tgcgatcggt
    17041 caagcgaacc gaatcccaat ctactaaata aatcaagcca ctatctgtct caatccaatt
    17101 actatgtcgt acatctccat ggacaatggt cgcatggtcc tctctaaatc ctggaatagt
    17161 ctgacgtaaa tcagccatca cttcactgat aaaatgattt ttacgcaaag catctggagc
    17221 cgtttcctgc caagactgta gtaaatctac aggtgtttcc atggcatagc ccaaacgact
    17281 caattgtgtc atcaacggac gtgagcgatg aagacgggtt aaaatattga cgatttgctt
    17341 acgattcata tcataggggg tcaatatctt gcctgtcaac cattcttgag cacacatatc
    17401 acgcccatct gccaaacgac gactccataa taattgtgga gcaatttgtt ctctagctag
    17461 accaggtagg attggagagg tgttcatttt tacaaagatg cgcttcccat caggatagct
    17521 acccatataa gccttgccac ttttcccagg tatgggagtc agtgttagct cattatcacc
    17581 caaatccatt tctttcctcc gtataaagtt tccttactat tctactagtt tttggcctat
    17641 tcgtcaacca ctctaacccg attctcaaag aaaagcctct ggagtggttt gggttatcat
    17701 tatagaaaga aaaaggtaag cacaatctcc tacttacctt ttcatgattt ttataaataa
    17761 tataagagtg gccaactgtt gtaaagcata tggactagag tagaaaccca taggttttgg
    17821 ctctttttat aagcaaagcc cagcaacaaa cccccaagta gataaaaaat aaacgaaggg
    17881 acattagaag gtccatgcat gaaagaaaag agagaggaag ttagcacaag ccctaaccaa
    17941 gaattgtcaa aaaccgcacc ttgaagaagt cctctgaaaa tgagttcctc ctggatgggg
    18001 ccaaaaactg aggtagtcaa aatcaaagac agggaaattc ctctgctgac ttccgccaag
    18061 tgttggaccc ctgagccaga agaaacagcg aagaaatgaa tataaaccag cgtctcaagt
    18121 acacttagaa gaaagattgc aatgaaaagc aaaagttgtt tcttgcttaa catcccaaaa
    18181 gaaatcattt caaactttct agcataagca acactcagcg aggttacaag aagacttaca
    18241 ataatcaatg cagattcatt ttgaaaaaaa tccccatggt taatcgtata aggaactgta
    18301 atgacaatta ttagtactaa aaatccaaaa cacaaggcat gaaatttatc aaaaaactcc
    18361 ataaaattat cctcctcaac aaacagactt cctacacgtc attctttaga tctagccttt
    18421 ccaaaacaaa ccattttagc aaccaaaatc cgaccacata gccagcccct atgaatatag
    18481 ccattaaagc tagcatggaa tttaggaaat taaaaaccac cgcagataca aaggttagca
    18541 caaaaacatt aaaagcaatg gtgtcagaag ccaagactat aatatagggt gtcaaccgat
    18601 ctaaagtttt ggaatctagg aaaaataagt gtttatacat gatgacctcc tctatggctg
    18661 aaaagcaagc cttttgtttt ttttacccca agaccctatg tagaaaagtg agcaaaaacg
    18721 ggaaggtcgc tacaatatta ttgatcacat gcaccgcata ggatggataa atactcttgg
    18781 tatagcgggt caaaccggca aagatgattc caaatgttgc aaagacgaag atatctaaca
    18841 gactaggcag actcaaaaaa tgaggaagag caaacaaaat ggaaggaaga agcaaatcaa
    18901 ggccaaatcg cgaatgctta aagaaggcgt gttgcagtaa tcccctataa atcaactctt
    18961 ccattagtgg agtcaggaaa aacagggtta catagatacc tagctcagca aagttggtcc
    19021 cactataacc gatcaatacg gcccaacctt cagcggttga ctgaacatgt ttagctgtct
    19081 gaacgttaaa agagatatgg agcactacca ctaatactgt caaaatagaa taccaaagcc
    19141 atttttttct tggaatgcga aagagatagc catggcctgt cttaaccaga atccaaatca
    19201 tgactccgct aaatagcaga ctcaagatat ttctaatcca gaaaaaattt cctacctgag
    19261 aagaaaattg ccaatgattt tggacgataa gagttaactg agaaagacca aatacgaaaa
    19321 ataggtaaga caagactgca cttattttga aaacaagctg atactttttc atagaaattc
    19381 ttcctcattt cttgtatttc agcctatatt ccacataaat gaaaacctgc tcctatataa
    19441 ccacggtcat gataaccgcg cgggcagtaa ccacttactg gaaaaggacc tgaatttttt
    19501 aatataggag ggtaagaacc gaatggactt acctgattag cgtaccctct ataaatatca
    19561 gagacatccc caccctctat acgagcaagc atttcattat ccataactgc aaattgtgac
    19621 atcatttttg tatccatgac gaatatctcc ttttttatgt ttaatttttg tcctgttgca
    19681 accttgacaa gtttagtata tcatcgttta ttaatttttt tcatccaaat cttgaattgt
    19741 catcgaaacg tcttgaattg ttaaaaaatt taaaaagcaa gcattaacaa catacttgcc
    19801 ctttcactct tgtctatagg caaattctag ctatatgtta ttacaaacaa atagtatcat
    19861 ttcacctgtt taccatctat ttttatttat gaattttttg tttcacttta atcattatag
    19921 aagccataaa aaacgcgaca gctagtcgaa tcagttggta actttcttcc tcgcctgttt
    19981 tcagtaataa tttttcattc gaagtaatag attcgcttac ataatcagca tccttaacat
    20041 cagttaaaga agttttattt tcttgagcag catcaaaaat aggtttgaga gtttccaatt
    20101 gattcttatt gtctaacatt gctaaattgc cagattgagt agttttagct tcagaagttg
    20161 agtttgtact ctccactttc ccactatttt cactattagc attggatatt ttcatttcag
    20221 ttcttttttt atcactcata cctttgctgt cacttacaac tttatcacta atattcatgc
    20281 tgtcacttct agtattctca tcacggacac tttcagttac tgtttcctca gaagatttca
    20341 ctgtctccgt ttgcttaggt gagtttctcg aatacatata atcatacacc tgttgtgcag
    20401 ttgctccgcc accaacccat ccagatgtcg gatgtccatt tcttaaatac aaaattgttg
    20461 gtgttcctgg aataccgact tttttaaaat agaaactctc tcgcttcccc atcaaaatcc
    20521 ttaccatcca aatcataata ctctaacttc ttttcaatca agttattaaa ttctttcaat
    20581 tcaggagaaa attgacgaca atgagagcaa gtttttctac caaaataaat agtatgttcc
    20641 aattgatccc ctgtaaaaga ttgacgaaca gcttcaatat ctatcttttt aaagtccgta
    20701 acattcttct catactcttc tggggttaca acagaaggct tatgctccac cttttcctta
    20761 gccgatggca aatctggcgc cacaagagaa ctatcattcg cctgggtaga tgaagtagta
    20821 ggctgttctt ctgcatatgc aagacctgca gtaatagatg caaatacaag tggtacaagt
    20881 aatagcgatt ttatcttttg tttcttcata agaatactcc tttacatttg ttatctttat
    20941 cttaccttat ttaagattac aaattcatct aaatcttaaa tggtcatcga aacatcttga
    21001 attgcaaaaa cttcattata aaagggtaag tatttcaaaa acacttaccc ctttatactt
    21061 tattaagctc tactttttat aatacttcaa tccccaaatg agcagaagca tgatggtcaa
    21121 gcagagaata gcgccaatgt aggcaattat ttgttggtag gattctcctg ctgtgatccc
    21181 tctatacaaa caaataatag acataaaacc tgtcaagccg atgaacatga gttgattagt
    21241 tctaggacta accaaatcat cgtcttcaaa ctcttttatc cgcatttccc tagtgaggta
    21301 aacagtgacc aaaatagaag ctaagttaat aaccactaaa aaaaattgga aaactaagga
    21361 aaaatttaaa aactgacgag atagaaatag ataagtagag acaagcaagg gcaactgacc
    21421 taagaacaat ctcgcaagga agatgttccg ttttttagca agaaaaattt tcatttcttt
    21481 tctcctttct ttttattgat agcaaaatag atcataactg caatcacata ggctatggta
    21541 taaaatagct gataccaagc actctcccta agcggatata gaaagatgga catgattaga
    21601 tacagaacga aaataatcag tatttttttc ttcataagat ttcctcctaa atgtgtgctt
    21661 tatcttagtt gagcaagaac atttacactg ctagtatagc acttattttg accttggatc
    21721 actcaaatca taaatggtca tcaaaacctc ttgaattgta aaaattaaaa aaagcaagca
    21781 tgaaaaacat actttcctct ttatattgta ttgataccaa cttgtttgta gacttttcat
    21841 cctgctatca catatcattt tgacaggcga aacaaaatta aagaaactcc cctgtaaatt
    21901 aagctagcaa atacagggga gaaatttatt ttttaggggg tactatctgt atcctttttg
    21961 ggagattttg aaaatatttt tctaattaaa tcatccatat aaggaccaaa tataccaact
    22021 actaaaccaa taataaaact tttaaaatcc ataattacca ccaacatgtt gctgcatagg
    22081 ctacacctcc aagtatagct ccacctgcag caccagttac acctattcct atagcaaatg
    22141 gtcccaatag aaatgtcaaa ccgttgttac acacccatca attgcgccat atgcaacccc
    22201 tgctgcacaa ctaatttttc ttccccaatc aatatctcca ccttcaacgc aagcaagcat
    22261 ttcattatcc ataacagaaa attgtgacat catttttgta ttcatatagt atgtcacttt
    22321 tcagttacgg aacaagttta atataaaaat tatcaaaaaa acataggcaa taaagagaaa
    22381 aattaattta tcatagatta gaaataatat gacaaaacaa ttcaatgatg ttaattcaat
    22441 agtcttttgt tttttatcag agatacttat ggatagataa ataagatagg tttgaaaagc
    22501 gaagagaata ataaagaata tagccttcat aaaatttagc tttcattttt atgatgtagc
    22561 ggtataggct aaatatccac aaaccactgc tcctcctatt gcagcgcccc atggtcctag
    22621 aattttccca tatttcactc cacccgctgc acaacctaaa gcagcaacta cagctgctcc
    22681 tccggaatta cctccgtaaa cctcactcag cattgtttca tttatattac aataagtatt
    22741 catacaagtc tccttttgtt aaaatccacc cgttgcccct gttactcctg cccaaagagc
    22801 cacaccaaat ttagctccta tgtatccaca tgctcccata aatggtgctc caacaccact
    22861 cgcagcacaa atagctgtcc ctaaccccca gccaccaaaa gcggcaccac caccttctaa
    22921 gacattagtt tgccaattat tcttgcctcc ttcaatacta gataacatag ttatatccat
    22981 aatctcaaat tgttccatca tttttgtatt catgacaaat actccttttt tattttttat
    23041 ttttgtcttg ttgtaacctt gacaagttta gtatatcatt attttttaat ttttttcatc
    23101 caaatcttga actgtcatcg aaacgtcttg aattagcttt tttatttcaa gccacctcta
    23161 aatgtttaaa aaaaataatt tctaatcact tttttaccat tcaggaagtt ttaatgacta
    23221 ttcaagattt cataaaatat gaacttattt ttatggcata atatacctat ccactatatt
    23281 aaaggaattg ccaatgactt cttataaacg tacatttgtt cctcaaatag atgcgagaga
    23341 ctgtggtgtc gctgccttag cctcgattgc taaattctat ggttcagatt tttctctagc
    23401 tcacttgaga gaacttgcaa agaccaataa agaagggacg actgctcttg gcattgtaaa
    23461 agccgctgat gaaatgggct ttgaaacaag gcctgttcaa gcagataaaa ctctctttga
    23521 catgagtgat gtcccctatc catttatcgt tcacgttaac aaagaaggaa aactccaaca
    23581 ttactatgtt gtctatcaaa caaagaaaga ctatctgatt attggtgatc ctgacccttc
    23641 tgtaaaaatc accaaaatgt caaaagaacg ctttttctct gaatggactg gagtagctat
    23701 ttttctagct cccaaaccca gctatcaacc ccataaagat aaaaagaatg gtctactaag
    23761 cttccttcct ctgattttca aacaaaaatc tctcattgct tacattgttc tctcaagctt
    23821 attggtcact attatcaata tcggtggttc ttattatctc caaggaatct tggatgaata
    23881 catcctaaat cagatgaaat caactttagg aatcatctca gttggtctgg ttattaccta
    23941 tatcctccaa caagtcatga gcttctccag agattatctc ctaactgttc tgagtcagag
    24001 attaagtatt gatgtgattt tatcctatat tcgccatatt tttgaacttc ctatgtcttt
    24061 ctttgcgaca cgtcgtacag gagaaatcat ttcacggttc acagatgcta actctattat
    24121 agatgccttg gcttctacca ttctttctct ttttctggat gtttctattc tgattcttgt
    24181 agggggagtc ttactggcac aaaatcctaa tctcttcctt ctttctctta tttccatccc
    24241 tatatacatg ttcatcatct tttcttttat gaagcctttc gaaaaaatga accatgatgt
    24301 catgcaaagt aattctatgg ttagctctgc cattatcgaa gatatcaacg ggattgaaac
    24361 tataaagtcg ctcacgagtg aagaaaatcg ctatcaaaat atagacagcg aatttgtaga
    24421 ttatttggaa aaatccttta agctcagtaa atattctatt ttacaaacga gtttaaagca
    24481 gggaacaaaa ttagttctga atatccttat cctatggttt ggcgctcaat tagtcatgtc
    24541 gagtaaaatt tctatcggtc agctaattac ctttaacaca cttttttctt actttacaac
    24601 tcctatggaa aatattatca acctccaaac caaactccaa tctgcgaagg tcgctaataa
    24661 ccgtttgaac gaagtctatc tagtcgaatc tgaatttcaa gttcaagaaa accctgttca
    24721 ttcacatttt ttgatgggcg atattgaatt tgatgacctt tcttataagt atggttttgg
    24781 acgagatacc ttaacagata ttaatctcac gattaaacaa ggagataagg ttagcctagt
    24841 tggagttagt ggttctggta aaacaacttt agccaaaatg attgtcaatt tctttgaacc
    24901 ctacaaaggg catatttcca tcaatcatca ggatattaaa aacatcgata aaaaagtctt
    24961 gcgccgtcat attaattacc taccacaaca agcctatatc tttaatggct ctattttgga
    25021 aaacttaacc ttgggcggta atcatatgat tagccaagaa gatattctaa aagcttgtga
    25081 attagctgaa attcgtcaag acattgaaag aatgcctatg gactatcaaa ctcagctctc
    25141 tgatggagct ggtctatcag gaggacaaaa gcaacgaatc gctctcgctc gtgctctttt
    25201 aactaaagct cctgttttaa tactagatga agctactagc ggtcttgatg tctttactga
    25261 gaaaaaggtt atagataatc ttatatccct aactgataaa accattctct ttgtagccca
    25321 tcgtctcagt atagccgaac gaactaaccg tgtcattgtt cttgaccagg ggaaaatcat
    25381 tgaagttggt agtcaccaag agttaatgca ggcgcaaggc ttctaccatc atctattcaa
    25441 taaataagga gaatgttatg aatcctaatc tttttagaag cgtcgagttt tatcagagac
    25501 gttaccataa ctatgcgaca gtgttaatta tacctctttc attactgttt actttcatct
    25561 tgattttctc ctttgttgcc acaaaagaaa ttactgttac ttcccaagga gaaatcgccc
    25621 ctacaagtgc cattgcatct attcagtcga ccagtgataa tcctatcctt gctaatcatt
    25681 tagtggcaaa tcaagtagtt gagaaaggtg acttactcat caaatactct gaaacaatgg
    25741 aagaaagtca gaaaactgcc ttagcaactc aattacaaag acttgagaag caaaaagaag
    25801 gacttggaat tttgaaacaa agcttagaaa aagcgactga tcttttttct ggcgaggatg
    25861 aatttggcta ccataatacc tttaggaatt ttactaaaca atcccatgat attgagctag
    25921 gtatctcaaa gactaacacc gaggtttcaa atcaagctaa tcttgccaat agcagttcat
    25981 cagccatcga acaagaaatt acaaaagttc aacaacaaat tggagaatac caggagctga
    26041 gagatgctat cataaataaa agggctcgct taccaactgg caatccgcac cagtcaattt
    26101 taaatcgtta tcttgtagcc gctcaaggac aaacacaagg aactgcagag gagccatttt
    26161 tatctcaaat taatcaaagt attgcaggtc tggaatcatc tatcgcaagc ctcaaaattc
    26221 agcaagctgg tatcggaagt gtagcaactt atgataatag tttagcaacc aaaattgaag
    26281 tactccgcac tcaattttta cagacagcct cacagcaaca actaaccgtg gagaatcaat
    26341 taacagaatt aaaagtacaa ctagatcaag cgacacagcg cttagaaaac aataccttaa
    26401 cagcaccaag taaaggtatc attcacctaa gcagtgaatt tgaaggtaaa aatagaattc
    26461 caactggtac agaaattgct caaatattcc ctatcatcac agatacaaga gaagtactaa
    26521 tcacttacta cgtagcttct gactatctac ctctactaga taaaggacaa actgtaagat
    26581 taaaactgga gaagattgga aatcactgca ttaccatcat cgcccaactt cagacaattg
    26641 atcaaactcc taccagaaca gagcaaggca atctctttaa attaaccgct cttgcaaaac
    26701 tatctaatga ggatagtaaa ctcatccaat atggcttaca aggtcgcgtc actagtgtaa
    26761 ccgcaaagaa aacatatttt gattatttca aagataaaat tctaacccat tctgattaat
    26821 tttcagataa cactctataa ctatttatta tcttatcaaa aaggagaatt gtaacatgga
    26881 taagaatcaa aacctaactt cattccaaga actaacaact actgaactca accaaattac
    26941 aggcggagga tggtggaaag atttattaca tagatttaat gtaattgaac aaaacaatac
    27001 aaaaggattt aatcaaccaa tacaactata aaaaataaga ccgagaaaca agaattctcg
    27061 gtcttatttt catcattctg tatgtatcat agtaagcacc tgacgaaaga cttgatcttg
    27121 acaggtagta tttaaactgg cattgggata actttccaca atcttcataa cggtatagag
    27181 accgacacct ctctcctccc ctttagaact aactccaaag gagaagattt cagaaatatc
    27241 gatgccctct tctttgatag aattttcaat gataaaggtc tcctgtgctc cattttttaa
    27301 aaaggcaatt gaaacatgag gttgactagc ctctgcactc gcttcaatag cattgtcaca
    27361 aaggatagac acgatggtta gaaaatcaag caggctcatc ccctcgacct gaatctcctc
    27421 aggaacttcg acattaaaga taatcttctt atctctagct tttagaaatt tccctgctag
    27481 aagacttttg agggcacggt cacgaatatt caccaatctg cccaggtcat atttattgtc
    27541 ctgcaatttc tgactggaat cctttaaaac agagtcgtaa acctctttta tctgctccat
    27601 atcctcctct tcaataccca gacgtaaact ggtcaagagg ttggtgtagt catggcgaaa
    27661 actccttact tccttgtaaa gttcctctat atgccgacta tggcgttcca tatctctata
    27721 gcgcaaggtc tgctcttggt tcagtctctc atggagtttg ttcttcaaat aggtatccaa
    27781 tttcttgata acccccataa aaaataggag gtagaacact aggatgagat ggcgaacagt
    27841 cgttgattga ataccttgct catattcaaa gtaagacaga ctttgtatca ccaaatagta
    27901 agcccccatt atccagttaa tcttagtcag ggacttttga aaacctgtat ctagaatctc
    27961 ccttctcaaa ctagtaaaat catagtccaa ccatttcaaa aaggctagag aaatgaagaa
    28021 attgaaaatt attatacata acccagtgaa tgagtagcca tcatatactt gcccttgtcc
    28081 caaaaatgga agcacaaaat aggagactcc tctataaaag agattcacca atatcattgg
    28141 aaagagacca taaaagaaaa ggagtttttt aggaagccct cttaacaata agaaagacaa
    28201 gcctatgccg tacaagggtt ccataaaata agataggtaa acatttccta ctatatagct
    28261 aatcattaca aaaacaaaga ccaacagtat cttcaaaaga aaggccttaa aaatcctctc
    28321 gaaagtaaga ttaattccat ccaccttaaa gaaaatgaca atttctagcc cattagtaat
    28381 aagtgtatgc aataatatcc aaacaatatt cataaactct cctagttcag tgtaagttat
    28441 taatagcctc agacacttcc ctgaccttat aacgcacgat cagacaactt ccaccattgg
    28501 gaaggaaaag gagtttttct ttcttatcca aatgcaccac atttgcagga ttgatgagaa
    28561 aagagcgatg gcactgcaag agacggggtt cctgcttgaa aacctcctct aaactcgctg
    28621 taaattccag cctgtctgtc ttggtataga gaataacacg atgggctctg ggagacattt
    28681 cgagatagta aacctcttta aaaggatatt ggaattgggc aaattttgat ttaaagtaaa
    28741 agcaatcttc cgccagactt ttactatctt gactattggc atagaggagg gctgtctcga
    28801 tacgagattc aaactcctct gccgaaaggg ccttatcaat gtagtccaaa gctgacactt
    28861 ggtagcgaaa ggacagaggc ataaactccg agtgagtcgt cacaaagacg attagggcat
    28921 aagggtcccg atcccgaatc tttctagcca cttccagccc cttcatctcc tcatttcgaa
    28981 tctcaatgtc caaaaagaat agctgatggg ccccccttct catgcacctc tgtcagcagt
    29041 tggtccggct tgccaaatac ctcaaaagag ctaggaatga tatgatgtgc tttcaaaagt
    29101 ttctcaatcg tcgtttcaat tctagtctgt tgggaaaaat catcttctaa aacaaatatt
    29161 ctcatcttct tactctccct ctttcaacca tttttgacga atttctctat agcgacgtct
    29221 ggcatacttg acagcgtatt gaccttcttc tccgagaaaa aggattcttt tttgaaaatc
    29281 aatcgatttt aatttatcaa gattaaccaa acaatttcga tgacatctca tcaaggtttc
    29341 cccatattgg ttctcaagat tacttaaatt ttgaagcata ttaaaactag cttcttctgt
    29401 aacaatctgt acggtatggg ctttagttgg atgtgtttgg atatagtaga tatcgtcgac
    29461 gtttacttta tagactgttt tctgcgtctg aataatcata tgtttcattg gattctcctc
    29521 ctaagaaaag aataacagaa aagttctagc ccaagcccac ccaatcctaa acagtctttg
    29581 aaagctccta aaaggattat ttttctacaa catcatgatg agcttacaac tattttatca
    29641 taaaattcac tcagcgcaat taaagatttg aataaaaaaa aagaattaaa aatgataaac
    29701 taacagtatc aaaacacagt tgccagaagt aagactggca cccagattaa aggaggaatt
    29761 cccatgacag atacagaccc tatcaaaaga gctcaggctt tgattactga cttaaacaaa
    29821 gcctatcaag catgcaaaca ggcaagcgct gacgacgtcc gctttcagga gcaattaaac
    29881 tctattcttg gttttctagc caaggctgag acagcggata atcgagtctt gattgaactg
    29941 gaaaaatttt accagacttc cagtcttctc atgggactca gcgctcttga tccagatgct
    30001 ccaactcgcg ccgcttggcg ggcctatgac cgcttccact ttgaccaagt caagaccaag
    30061 ttgagcctct acggtccaac cattatcttg taaaaaataa aagaagctga gacagattga
    30121 actcaacttc ttttttgttc catataagca atgttagtcc tgcatctgac gttttacctg
    30181 ataaggcaaa tacaagactt gtaaaaccaa acctgcaccc agcaaggcta gaagggctag
    30241 tttttcttgg aaggtcacaa ggccgacaac taattccacc actaagacca aactagtcaa
    30301 tcctcggact accgcctgca agcctttttc cttttgcccc ttgtccagcg gaaaaagttg
    30361 ggtcaaatac tggtagtcaa aggcgtgata gagggccagc aactgaaaga gcaagaggta
    30421 gttaaacaga accactaccg ctgtcgcaat ccaagattgc tcaataaaga cctgcgccag
    30481 caaagaaagc aagagcagac ggagactaag ggcaaaaagg tctccatttc gcaaataaga
    30541 acgcagatag agattttgcc aaatcttccc aggcaccttc tgaacaacct ttagaataaa
    30601 gtccagatag gcacgacgct tgacgctgtt tgaaattccc ttgacctgcg taaagagggc
    30661 aaagaaacga agcaagactt gcttacgctt gctttcttgg gaaatgacat agtcccaatc
    30721 cagtccagtt tcagtgaaaa atttgctggc cttttgacga aagaggaaat attttcctac
    30781 ccccaataaa agcacataaa ccagaaaaac aggcaagcca taacccatgg ctaaaaacaa
    30841 gggcgcaaat aaaagcaaga agagggtctg gacaaaaatc caaaagacta gggaaatgcc
    30901 ggtttgacgc ttgagatgga gcttgatttc ctcttctcca actaagagaa agagcttgtc
    30961 tggagcctcc atataggtgg caattcctcc ccaaagtaaa agtaaaacag acgtaattcc
    31021 tacaaacaaa aggataggcc aatgattttc aggaaaatgt tgtaagagtt gactgtactg
    31081 gtaggctaga aagcccaaca gtacaagcag gaacaagaca aagtggtcat tgagaacata
    31141 gcgcagataa ccaagacact ccttacgaaa ggcctgcttt ctctttaaaa acaagtcttt
    31201 cataggtcct cctcttttgt cagagccaag taaatatcat tcaaactagc ctcaggcata
    31261 tcaaaggctt cgcgtagttg caggagattg cctttggcac gcacctctcc cttgtgaaga
    31321 atgacaaagg catcacacat cttctccgcc gaatccagca cgtgggtact catgagaata
    31381 gacttgccct tttgcttctc cacttccaaa agctgaatca aatcagaaat agccagcgga
    31441 tcaagaccaa ggaaaggctc atccacgatg aaaagacttg gatccaccac aaaagcacag
    31501 ataatcatga ccttctgctt catccctttt gaaaaatgaa cagggaacca gtctaatttc
    31561 tgttccaaac ggaacatttt taacaagggc tctactcgtt cgaaagccac tttttgctca
    31621 ataccgtaag ccatagcaac cgtttcgata tgctctctga gggtcaattc cttatacaga
    31681 ctaggcgtct caggaatgta gccaatctgc ttgcggtagc tagtcgcatc tccttgcaga
    31741 gtcaggccat tgatattgat ggagccacta taaggtgcca acagaccgat aatctcattg
    31801 atcgtcgttg atttcccagc accattgaga ccaatcaaac cgaccaactg cccactttca
    31861 acagtaaagg acacatcttt caaaacagga acatgaacat agccacctgt caggttttta
    31921 atttctaaca tattttctcc aaatctggta taatgtagct atattatatc aaaattcagt
    31981 acagtagagg tagattttat gtcagattgc attttttgta aaatcatcgc aggggaaatc
    32041 cctgcttcaa aagtatatga agatgagcag gtccttgcct ttcttgatat ctctcaagta
    32101 acactaggac acaccttggt cgtgccaaaa gaacactatc gcaatctttt ggagatggat
    32161 gctacgagcg ccagccaact ctttgcccaa gtaccaaaag tagctcaaaa agtcatgaaa
    32221 gtcactaagg ctgctggtat gaatatcatt tccaactgtg aagaagtcgc tggtcaaaca
    32281 gtttttcata ctcacgttca ccttgtgcct cgctacagtg ctgacgatga cctcaagatt
    32341 gattttatcg cccacgaacc agactttgac aaacttgctc aagttgctga aaccatcaaa
    32401 aacgcttaag gagttgctat gaaattatcc aacctactgc tatttgcagg agctacagcc
    32461 ggaagttatc tggttacaaa aaatcgccaa accatcacag atgaaatctt gaataccact
    32521 gaccgcgttc aagctatcaa ggacgatgtg gatattatcc aaaacagcct gcaaatcatc
    32581 gaccagcaaa aagaacttat caaggaatac caagaagact tgacttacaa gtttaagatc
    32641 ttggaaaagg atatccagac tagactagct gtgataaaag aaatgcaggg aactgaagat
    32701 aagtaaaaag agcccccggc tcttttttaa aacgtaaaca agttatagtg aattgaattt
    32761 agaatagcac atcatagttt ctaaagcatt gttagagatt actttaaaat cctcttatca
    32821 atttgttcat attctatttc aatctactat atatatccca tcaactatgc atcataattt
    32881 aggtaactca tactcaataa aaatcaaaaa gcaaactaga aagctaggca cagactgctc
    32941 aaaacaccgt tttaaggttg tggatagaac tgacaaagtc agctcaaaac actgttttga
    33001 ggttgtggat agaactgacg aagtcagtaa ccatacccat ggcaagccga cactgacgtg
    33061 gtttgaagag atttttgaag agtatcattc accatttcac aactaaatcg aagactttct
    33121 gccgtaatga aaacacctga aacagcttgg tttcagctgt ccggaaactt taagacttag
    33181 gttcaaagtt taggtatgga acttcgaagg aggtcgctac cgtccgtcat tacttaggaa
    33241 agtcttaaaa aatctataaa gtaaaaagag cccgacagct ctttttgttt atactcaatg
    33301 aaaatcaaaa agcaaattag ccacgggctg ctcaaaacac tgttttgagg ttgtagatac
    33361 gactgacgaa gtcagtcaca tatatacggc aaggcaacgc tgacgtggtt tgaagagatt
    33421 ttcgaagagt attattctcc atcaaaggca tctttaatat ggtcaaagaa gccttttttc
    33481 tttggattta ctttcaagtc accagcagcc gcgaattctt tcaaggctac tttttggcgg
    33541 tcgttcaagc ctgtcggtgt tacgacatta acagtaacgt attggtcacc aactgcaccg
    33601 ccacgaaggc tcggtgcccc cttactacgt aggcggaact tcttaccagt ctgagttccc
    33661 tctggaataa ccaattcaac atcaccgtga acagttggaa tatctactgt atcaccaaga
    33721 gccgcttgga caaagttgag gttgagattg tagaagatag tcgttccttc acgttcaaac
    33781 ttgtcgctag cttccacaga aactactaca tacaagtcac cataaggtcc accgttaaag
    33841 cctgcttcac cttgaccagc gaggcgaatt tgttgacctg tttccacacc agcagggatt
    33901 ttcacatgta cgctatgagc ttgtttctca tgacctgttc catgacaggt tgtacatgga
    33961 tatttgattt cttttcctcg accgtgacag acatcacagg ttacttggcg acgcatcata
    34021 ccaagaggag tctgcgtatc gacgttaatg acaccagcgc catgacagcg tccacaagtg
    34081 actggactcg tccctggctt agcaccagag ccattacatg tacgacagcc agcttcacga
    34141 tgatacttaa cttccttctc agttccgaag atagcttctt caaaggtcaa attgacacga
    34201 tactggagat catctccttg gcgaggagcg tttggattgc gcgaagaacc gcctccgccg
    34261 aagaaacttg agaaaatatc ctcaaaacca ccgaagccac ctgccccatt gaaaccgccg
    34321 aaaccaccag ctccaccaaa accaccattg gcacctgcag caccatactg gtcataggca
    34381 gcacgttttt ggtcgtcact caaagtctca taggcttctt gaacttcctt gtacttgtcc
    34441 tcagcaccag gctccttgtt gatatctggg tgatattttt tggaaagctt acgataagcc
    34501 tttttgattt cgtctgccga agcgtttttg gataccccca gacgatcata aaattcagta
    34561 ttgttcataa ttcaagatac aaagggcgtc aaacggacac agccatctag gagttttgac
    34621 gacggacgct tacgtcctag agaaaactat ctttttggca cagtccgtag cccgtgttca
    34681 gttacgaaca cccttattcc tttctattga tgttctgaat cgaaccacca tttaggtcgg
    34741 attcaattcc tttcttttat atttagtaag aaattctgaa acccgtgttc agttacgaac
    34801 actctttgtt cctttctatt gatgagacaa aacccaaaca aatgagattc cgtccctttt
    34861 tatcgaaaaa cagaggctgg gttgcaacct ctgcaaataa gtaaattaac tttattttga
    34921 ttttaaatat cctataagta cagaaatatt tatatatttt tgtctcttct tagtatttgc
    34981 tatataattc caattgatca taagaaatac aactactgtc caaatcacac caattatgaa
    35041 cggtattaca aatttatatg tttgtatttc taatgatatc atagctgata ccagcgccga
    35101 tacaaatgca gacaacaata ctgaggtaac aaaattcctt accgcttcat catttttgat
    35161 acaactatca tgttcagcta atataatttc tctatatcta gctaaatatt ctattggaat
    35221 attatcaata aaatcttttg atgttctata gttatcagct tgattacaaa aatcattata
    35281 aacttttcgt gtatttttta gatactcttc atccaataca ctcgtcttac ttttccgtaa
    35341 actctccgtc tacgacgtca tcgcctgcgt ttcctgtttc ttgtgcgcct tctgctcctt
    35401 cttgagcttg ttgcgctgct gcggcttgtt cgtagagttt aacagcaagt ccttgagctt
    35461 tttcgttcaa tgcttcaagt tttgttttca tgtcgtccaa gttgttgtct tcttgagctt
    35521 tcttaaggtc atcaagggca gcttgggcag cgtcacgttc tgcgtcgaag cctttacctt
    35581 cagtttcctt gattgtcttt tcagtcgcaa agattgcttg gtctacttca ttacgaaggt
    35641 ctacttcttc tttacgtttc ttatcggctt cagcgtttgc ttctgcatct ttcatcatgc
    35701 ggtcgatttc ttcgtcagtc aaacctgagt tcgattggat gacaatagtt tgttcttttt
    35761 gagttccaag gtctttggcc ttaacagaca cgataccgtt cttgtcgatg tcaaatgtta
    35821 cttcgatttg aggaattcca cgaggtgcag ctgggatatc agtcaattgg aagcgtccaa
    35881 gagtcttgtt atctgctgcc attgggcgtt caccttgaag aacgtggata tcaacggctg
    35941 gttggttgtc tgctgctgtt gagaagactt gtgatttaga tgttgggatt gtagtgttgc
    36001 gatcgataag ttttgtaaat actccaccca ttgtttcgat accaagtgac aatggcgtta
    36061 catcaagaag gacaacgtcc ttgacatcac cagcaatcac accaccttgg atagccgcac
    36121 ccatagcaac tacttcatca gggtttactg atttgtttgg ttctttacca gtttcagctt
    36181 taacagcttc aacaacggca gggatacgag ttgaaccacc aacaaggata acttcgtcga
    36241 tttctgacaa gctcaaacct gcatctgaaa gggcttgacg aactggaact tttgtacgtt
    36301 caacaaggtc acgagtcaaa tcgtcaaatt tcgcacgagt caaagtcatt tccaagtgaa
    36361 gaggtccagc ctcacctgca gtgataaatg gcaagctgat ttgtgttgaa gttacaccag
    36421 aaaggtcttt cttcgctttt tcagccgcat ctttcaaacg ttgcattgcc atcttgtcag
    36481 tagacaagtc gataccgttt tctttcttga attctgctac caagtggtca atgatttttt
    36541 ggtcaaagtc gtcaccacca agtttgttgt cccctgcagt tgacaatacg tcgaagacac
    36601 cgtcacccaa ttcaaggata gagacgtcga atgtaccacc accaaggtca aataccaata
    36661 ttttttcttc tttgtcagtc ttgtccaaac cataagcaag agctgctgca gttggttcgt
    36721 taacaatacg ttctacttca agaccagcaa ttttaccagc gtcttttgtt gcttgacgtt
    36781 gagcgtcgtt gaagtaagct ggaactgtga taacagcttt ggttactttc tcaccaaggt
    36841 agtcttcagc gtagcctttc aagtattgaa ggatcatagc tgagatttct tgtggagtgt
    36901 attcttttcc atttgcagaa actttttcag aagttcccat cttagatttg atagagataa
    36961 ctgtatctga gtttgtgact gcttgacgtt ttgcagcatc accaacgatg atttctccgt
    37021 ttttgaatga gactacagat ggagttgtgc ggtttccttc tgggtttgcg atgattttgc
    37081 tttcagttcc ttcaagaact gcaactgctg agtttgttgt acctaagtca ataccgataa
    37141 ttttagacat gtgtttttct ccttgttagt taattttcta tttttctttt tgatactctt
    37201 cttaagtggc gacgccgtca gagcttgact tcgtcagtct ctttgactaa actttgagcc
    37261 taaggtctca aaatttgcgc aaatagcgcc acggcgaaga gtatcgtctt tggttcgctt
    37321 cgctcactat tacaaagcaa aacatatttt atctttcttc atagtttatt gtcgtttcgg
    37381 acaagttttc tattatgtaa attgcgacac gaggagtcga aatcgatttt atttcgacga
    37441 cgagttagta aggaagctag gcaaacgcca tagcaattgc cgttttctga cgagttgctt
    37501 tagctaccgt cagaattgcc gaatcgaaca cgccctaagc tctgagtaaa aataggagat
    37561 tttcttgtgt tcatcgaaca cttcgtcaat ctcctatttt tactgcgagc tttttacggg
    37621 ctttgtatct tagttataca ccactaccat tgctgggcgt aggatgcggt catggagttt
    37681 gtagcctttt tgaaagactt gggcgatggt atctactggg tgttcatcgt ctgctgggag
    37741 agtttggatg gccatatggt agttatggtc aaattcgcca tctgctgcga tttcttcaat
    37801 tccttcttct ttcaaagcgt gaatcaagct ttcttgcacc atccccaagc ccttcttcac
    37861 atcatctgtc aaaccttcaa ctgcaagtgc acgctcaagg ttgtcaagag atggtaagat
    37921 tgcttttgcc aagtcctggc tacgataacg ttgcaagttt tgacgttctt cattggcacg
    37981 gcgttggata ttttgcattt ctgcatgagc gcgaagatat ttgttttcga actcatctgc
    38041 acgttcattt gccaagtcca actcagactt ttcaggagtt gtttcttcag ctgtttccac
    38101 aacttcctct tcttgaactt cttctacttc ttcatttttt atatcttggg ccatgttcgc
    38161 ctcctttaat gatttcaatc ttaatgtact tcgtaatgat tactgctgag gtagaggtaa
    38221 aaatctgtca acttcatggt caaaacacgg ttgaccacat tgacttgatt gattagctgt
    38281 tggtaatcca gattaaccgg accgataatg gctagaattc caactccccg ataaggaatg
    38341 aggaacttac tactaatcac tgctaggtca gctaaacagg attcttgacc gtctgcaaca
    38401 cgaacatttt gcatctgatc ctcacgcaac ccctcacgaa tctccaaggc caccttttgc
    38461 ggttggtcaa agaactgata ggctgctaga ttggcaaaat tcaagagatt aaccttgcca
    38521 gccaccacaa tgttttcgtt gaacatttcc ttaaagatgt gttcaaagag atcaatcaca
    38581 ttatccgttg ttgtaaagta acgctggata atctgcggaa tctccgtccg aatcttgtag
    38641 tgaatatcta aaacagtgtg accgaggaaa cgttcctgaa tgattgcctt cagtttcagc
    38701 aaatcctcct gcaagaagtt ccttggaatc agaaactgac tagtaaccgt tcgcgactcg
    38761 tctagggtaa ataccgccaa ggctgtatgt tgccccaaaa caacgatatc aaaggctgtc
    38821 aaccgttgcc tgctcggctc aacatccagt gccactaccg tacagccact caggtctgtt
    38881 agcaagttag cagcctcttg cagaatatcc tccaatttga agaattcctg atcaaaggct
    38941 ttgacaatct catatacctc attttcagcc agccggtcaa aatccagtga gtgtttcaca
    39001 tagtactgaa aaccagcaac acttggcatc cgaccgcttg aagtatgagc cttctcaagc
    39061 aacccttgtt tttctagttc cgccatgtca ttacgaatgg ttgcactgct agagttaata
    39121 gactcttgca aggcttttga tccgacaggt tcgtgcgttt tggtaaagat gtcaataatc
    39181 agatttaaaa tatcctgctg acgctctgta accatctcat cactcctctc tgtctgatca
    39241 attagcactc aaggcatcca agtgctaact tatgattcta ttatacaccc ttagaagaga
    39301 atgtcaagac aaaaactcaa aaaattagca ctcttttatc aagagtgcta aaaatcaatc
    39361 tgaagaccta gaaccttgct attcatctac tcggtatttc ctttttagtc tgaaataacc
    39421 atgaattaga taagaaatca gaaaagcata taaaacaaaa ataagaaaga aagacataga
    39481 aagatcttct ctaaacgaac tcatacaaac aacaagacca cctaagaaag tagctgtaca
    39541 ggaacgaagt ctagatcgca taacttccca tttgagcccc ttactcatat agacttgatc
    39601 ctctggaagt aaatcagaag acgatttacg aagaatcgaa caagaacctg ctcctatcaa
    39661 ttcccaacct ctatctctaa aatcttgcag ttcatgctta tattttttaa gaaatctaga
    39721 atcatagata cggtagatga catcgtctgg ttgaccttgc tcaaaataga acaaaccaaa
    39781 acaactagtt ctatacctcc aacctttcaa atgcatctca tgtaaatact cttcttcctt
    39841 atccaaatca acaatggtga aaatccgaaa ttctactctg ctattcattg tcctacccca
    39901 aaattagaaa acatgcctgg cgttatttat tagataattc tttccacttt tgactcaatc
    39961 tccaaaaaat ataagaaatc tgaatcgcaa aaactatcaa taaaacccaa tctattatga
    40021 aaatcaaaaa tactccccaa ctgaaagaac tacctccagt gacaaacttt gagaaaaccg
    40081 gtaatagagc taaaaagaga agcaaaatag gaagcatccg cattgttaaa atccgtttgg
    40141 cataaaaaaa tctttattta aacgaaaata ttatggcaaa atttacgcca gtttttgaac
    40201 ggctgatgta gatattttat actttcaaaa tgtttaaatg tgattattta tttttgaaaa
    40261 atagctcacc agcccgactg aaagtgctta tagaatgata ataagtcgcc tgccgaaaac
    40321 agcgaaaaat agcggtgtta tgcggagata atctgacgcg atgcgaaagt atattgcata
    40381 cttattttca acaatttagc agagtatttt tataagtgtg atataataga agtataattt
    40441 gttctgatag tttattttat ggagaagtag atttttagaa tgcggagggt tcaatatggt
    40501 tgagtttata aagtctaaga aagaaatgag tgaggaggat attaaagcaa atttcatcac
    40561 tcctgctatt gtatccaaag gatggaaaag tggtgagcat atcacttacg aagaatactt
    40621 cactgatggt cgaattgagg ttagaggaga taaggctcgt cgtaaagaag gaaaaaaatc
    40681 agactattca ctgtattacc aatttggaac tcgaattgca attgttgagg caaaggataa
    40741 taaacacagc gttcgagcag gattacaaca agctattgaa tatggagaga ttttagatgt
    40801 tccatttgtt tattcttcga atggtgatgg ctttattgaa cacgaccgta tcacgagaga
    40861 agaacgtgag ctggagttag acgaattccc tactcgtgaa gaattatttt ctcgtatgac
    40921 gaaggaaaaa ggattgacgt acgaaattac agaagctatc tcaactccat actatacaga
    40981 cgccttctca atgaaaacgc cacgctatta tcagcaaata gctatcaacc gtactattga
    41041 aacagttgcc agaggacaaa aacgagtaat gtttgtgatg gcaacaggaa cggggaaaac
    41101 gttcatggct tttcaaatta ttcatcgcct tcgaaaagct ggtttggcta aacgagtttt
    41161 attcttagca gatagaaaca tcttagtaga ccaaacgatg gctgaagact ttaggccatt
    41221 cgaaaaggta atgacgaaaa ttacaccaaa acttttgact gctcctgaaa aattaaattc
    41281 ttttgaaatt tatctagggc tttatcagca actaactggt gaagatggaa ctgaaacaca
    41341 ttatcaaaaa tttgacaaag acttctttga tttaatcgta attgatgaag cgcaccgtgg
    41401 ttcagctaag gaagacagta actggcgtaa ggtgattgat tatttcagtt ctgcgacaca
    41461 gattgggatg accgctactc ctaaagaaac caagaatgct tccaatacgg aatactttgg
    41521 tgagccaatc tatacttata gtttaaaaca gggaatcgag gatggttttt tggctccata
    41581 tcgtgttatg agggttaatt tagatgtgga tgtggatggt tatcgtccag aaactggaaa
    41641 agttgatgct aacggacaat taataggaga taggtactac ggcaggaaag attttgataa
    41701 aaccattgtc attgatgata gaacgcaaag agttgccaag tttgtttctg attatatgaa
    41761 gcaaaacaat gcacgatttg ataaaacaat tgttttttgt gttgatattg accatgccga
    41821 gcgaatgcgt gctgcacttg taaaagagaa tctagactta gtccaagaag actatcgtta
    41881 tgtcatgcaa gtaactggtg acaacgctga aggaaaagct caactggata actttatgga
    41941 tgtcaattct aattttcccg ctatggtaac aacgtctaaa ttattaacga caggagttaa
    42001 tgctaaaaca tgtcgtttga ttgttttaga ctctaatatc caatccatga ctgaatttaa
    42061 acaaattatt ggtcgtggca cacgtcttta tcctcaaaag gggaaagaat tttttacgat
    42121 tattgatttt cgaaatgtta ccaatctgtt tgctgaccct gattttgatg gtgagccagt
    42181 gaaggtgctg gaaacaggtg cgaaaacagt cagtggttct acgcctggtt tcgtaggtga
    42241 ggaaggtgac ccagtagaaa aatatatcgt tacagacaag caggttacca ttcttaattc
    42301 tactgttcaa gtattggatg aaaacgggaa actgattacc gaaagcctga ccgactacac
    42361 tcgaaagaat atcttaggta gctacgccac tttgaacgat tttatcacag tttggcatac
    42421 ggcagataaa aagaagctta tcttagacga actttataaa aaaggagttt atctagatgc
    42481 tattcgagag tcggagggaa tatcagaaca agaaatcgat gattttgatt tactcctaaa
    42541 acttgcctat ggtcaaaaag aattaaccaa aacggaacgt atcaataaac tcaaacaaag
    42601 cggatattta tataaatata gtgaggaagc gcgtgctgtt ttggaaattt tactggacaa
    42661 atacatggat aaaggtattg gagaactcga aagcattgaa acattaaaac ttccagaatt
    42721 tcagatatat ggtggaacct tcaaaatcat caatacttat tttggagata aaaaacgata
    42781 tttacaagca attaaagaat tggagcaaga gctatttaca gtagcttaat gaaaggaaag
    42841 tatgtcaatt acatcatttg taaaaagaat tcaagatatc actcgaaacg atgctggtgt
    42901 taatggtgat gctcaacgta ttgagcaaat gtcttggtta ttattcttaa aaatttatga
    42961 tagccgtgaa atggtttggg aattagaaga agacgagtat gagtcaatta tcccagagga
    43021 attaaaatgg cgaaattggg ctcatgctca aaatggggaa cgggtattga caggcgatga
    43081 attacttgat tttgtcaata acaagttatt caaagagttg aaagagcttg aaataacttc
    43141 aaatatgcct attcgaaaaa cgattgttaa atcagctttt gaagatgcga acaactatat
    43201 gaaaaatggc gtcttgttac gccaagtcat caatgttatt gatgaagttg atttcaacag
    43261 ccctgaagat cgtcattcgt ttaatgatat ttacgaaaaa attcttaaag atattcaaaa
    43321 tgctgggaac tcaggagaat tttatacgcc acgtgcagcg actgatttta ttgccgaagt
    43381 tcttgaccca aaacttggag aatcaatggc agaccttgct tgcggaacag gaggcttctt
    43441 gacttcgact ctgaaccgtt taagtagtca acgtaaaact agtgaagata caaaaaaata
    43501 taatacagct gtttttggta ttgaaaagaa agcatttcct catcttttag cagttacaaa
    43561 tctgtttctt cacgaaattg atgaccctaa aattgttcat ggaaatactt tggagaaaaa
    43621 tgttcgtgaa tatacggatg atgaaaaatt tgacattatt atgatgaatc caccttttgg
    43681 agggtcagaa ttagaaacaa taaaaaataa ctttccagca gaattacgga gttctgaaac
    43741 ggctgattta tttatggctg tcattatgta tcgtttgaaa gaaaatggtc gtgttggagt
    43801 tattttacct gatggttttc tatttggtga aggtgtaaaa actcgcttga aacaaaaact
    43861 ggtagatgag ttcaacttgc atacgattat taggttgcct catagtgtct ttgcaccgta
    43921 tacaggaatc catacgaaca ttcttttctt tgataaaaca aagaaaacag aagaaacttg
    43981 gttttatcgt ttagatatgc cagatggtta taaaaatttc tctaaaacta agccgatgaa
    44041 gtcagaacac ttcaatcctg ttcgtgactg gtgggaaaat cgtgaagaga ttctggaagg
    44101 taagttctac aaatctaaat catttacacc tagtgaattg gctgagttga attataattt
    44161 agaccagtgt ggttttccaa aagaggaaga ggaaatctta aatccctttg agttgattca
    44221 gaattatcaa gcggaaagag cggctttaaa tcataaaatt gataatgtat tagctgatat
    44281 tttgcagttg ttggaggata aataatgaca ccagaacaac ttaaagcaag tatccttcaa
    44341 agagcgatgg aagggaaatt agtgccgcaa aatcccaatg acgaacctgc aagtgaattg
    44401 ttaaagagaa ttaaagctga aaaagaaaaa cttatcagtg aaggaaaaat caaacgagat
    44461 aaaaaggaaa ccgagatatt tcgtggtgat gatg
//
LOCUS       DAVKDZ010000008        41983 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000008 DAVKDZ010000000
VERSION     DAVKDZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41983
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.008"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            623..802
                     /locus_tag="VWZ30_000434"
     CDS             623..802
                     /locus_tag="VWZ30_000434"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9334237.1"
                     /translation="MKTVTKQVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVRE
                     YITDSISTNTIEEAR"
     gene            1413..1697
                     /locus_tag="VWZ30_000435"
     CDS             1413..1697
                     /locus_tag="VWZ30_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334238.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVKKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            1690..1938
                     /locus_tag="VWZ30_000436"
     CDS             1690..1938
                     /locus_tag="VWZ30_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334239.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVGGYVLEYHGLPATQDVD
                     AFYDQNQKINEIIARVGKQFNLNTHEELWLNNHVAKQI"
     gene            2034..2252
                     /locus_tag="VWZ30_000437"
     CDS             2034..2252
                     /locus_tag="VWZ30_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334240.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDAFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(2535..4859)
                     /gene="pflB"
                     /locus_tag="VWZ30_000438"
     CDS             complement(2535..4859)
                     /gene="pflB"
                     /locus_tag="VWZ30_000438"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEU9334241.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGSTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIDPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDKYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            5207..6268
                     /gene="dinB"
                     /locus_tag="VWZ30_000439"
     CDS             5207..6268
                     /gene="dinB"
                     /locus_tag="VWZ30_000439"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU9334242.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIELMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVVLNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITMTGF"
     gene            complement(6415..7260)
                     /locus_tag="VWZ30_000440"
     CDS             complement(6415..7260)
                     /locus_tag="VWZ30_000440"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU9334243.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            complement(7291..7428)
                     /locus_tag="VWZ30_000441"
     CDS             complement(7291..7428)
                     /locus_tag="VWZ30_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334244.1"
                     /translation="MRYQTHYQLKQKLWLEPFGNRQAIGGNELIHSGLFQVYHLGFGS
                     S"
     gene            7515..8456
                     /locus_tag="VWZ30_000442"
     CDS             7515..8456
                     /locus_tag="VWZ30_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU9334245.1"
                     /translation="MLYVGIDVAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALEDTGHYAFNLLNFLHEQGYEVYTYTPLLIKEFAKSLSLRKTKTDKK
                     DAHGIALKLLSDPNREQFQHDNRQVELKILARHIHRLKKKQSDWKVQYTRCLDIIFPE
                     LDKIVGKHSEYTYQLLTRYPNPQKRIEAGFDKLIEIKRLTASKIQDILSVAPRSIGTT
                     SPAREFEIIKHYKRLIDKVEKCVNDLMAEFNSVITTVTGIDNRLGAVILAEIRNIHAF
                     DNPAQLQAFAGLDSSIYQSGQIDLAGRMIKRGSPHLR"
     gene            complement(8835..10724)
                     /locus_tag="VWZ30_000443"
     CDS             complement(8835..10724)
                     /locus_tag="VWZ30_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU9334246.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFRERTVEKSNLDYTIRLDNLPAKRGAELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVKEKEQSKQLLTWIFPSILSISLLLSICFYFIYRRKTAPSIK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVMDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLHLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGIQLKSSFEAVLKEMQEGVRKRVVFWGLPDYYRPLTGGEKALQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVLYYWKLQLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDQAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINFYVAYGWHSMFYHSTAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            10863..12428
                     /locus_tag="VWZ30_000444"
     CDS             10863..12428
                     /locus_tag="VWZ30_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9334247.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTITGLIIGLAIGVFRTAPLSENKVIYS
                     LQKLVGWILNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMGKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILHFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            12428..13168
                     /locus_tag="VWZ30_000445"
     CDS             12428..13168
                     /locus_tag="VWZ30_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9334248.1"
                     /translation="MTQVILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEEIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(13277..13492)
                     /locus_tag="VWZ30_000446"
     CDS             complement(13277..13492)
                     /locus_tag="VWZ30_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334249.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(14363..15613)
                     /gene="ilvA"
                     /locus_tag="VWZ30_000447"
     CDS             complement(14363..15613)
                     /gene="ilvA"
                     /locus_tag="VWZ30_000447"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEU9334250.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGISTYIKETSPEIEIIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHVQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(15663..15803)
                     /locus_tag="VWZ30_000448"
                     /pseudo
     CDS             complement(15663..15803)
                     /locus_tag="VWZ30_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
     gene            complement(15988..16224)
                     /locus_tag="VWZ30_000449"
     CDS             complement(15988..16224)
                     /locus_tag="VWZ30_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334251.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMNFEQFFKLAQI
                     SREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(16331..17353)
                     /gene="ilvC"
                     /locus_tag="VWZ30_000450"
     CDS             complement(16331..17353)
                     /gene="ilvC"
                     /locus_tag="VWZ30_000450"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9334252.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYIVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGLRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(17419..17895)
                     /gene="ilvN"
                     /locus_tag="VWZ30_000451"
     CDS             complement(17419..17895)
                     /gene="ilvN"
                     /locus_tag="VWZ30_000451"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU9334253.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(17888..19588)
                     /locus_tag="VWZ30_000452"
     CDS             complement(17888..19588)
                     /locus_tag="VWZ30_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU9334254.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(19788..21455)
                     /locus_tag="VWZ30_000453"
     CDS             complement(19788..21455)
                     /locus_tag="VWZ30_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU9334255.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(21458..21823)
                     /locus_tag="VWZ30_000454"
     CDS             complement(21458..21823)
                     /locus_tag="VWZ30_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9334256.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(21980..22168)
                     /locus_tag="VWZ30_000455"
     CDS             complement(21980..22168)
                     /locus_tag="VWZ30_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU9334257.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(22284..22460)
                     /locus_tag="VWZ30_000456"
     CDS             complement(22284..22460)
                     /locus_tag="VWZ30_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU9334258.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
     gene            complement(22571..22768)
                     /locus_tag="VWZ30_000457"
     CDS             complement(22571..22768)
                     /locus_tag="VWZ30_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU9334259.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            complement(23183..24727)
                     /locus_tag="VWZ30_000458"
     CDS             complement(23183..24727)
                     /locus_tag="VWZ30_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU9334260.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYDQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            24940..25242
                     /gene="gatC"
                     /locus_tag="VWZ30_000459"
     CDS             24940..25242
                     /gene="gatC"
                     /locus_tag="VWZ30_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU9334261.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            25242..26708
                     /gene="gatA"
                     /locus_tag="VWZ30_000460"
     CDS             25242..26708
                     /gene="gatA"
                     /locus_tag="VWZ30_000460"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU9334262.1"
                     /translation="MTFNDKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGFPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            26708..28150
                     /gene="gatB"
                     /locus_tag="VWZ30_000461"
     CDS             26708..28150
                     /gene="gatB"
                     /locus_tag="VWZ30_000461"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU9334263.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKTTILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMQTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            28274..29317
                     /locus_tag="VWZ30_000462"
     CDS             28274..29317
                     /locus_tag="VWZ30_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEU9334264.1"
                     /translation="MATMKAARWHAAKDVRIQEIEVPEVLPHQVKVAVKFTGICGTDL
                     HEFLDGPIFIPTDEHVYSGQKAPVTLGHEFSGEIIEVGSDVTCVKVGDRVAVEPILAK
                     NNLVGDYNLDPNLNFVGLAADGGFAKYCVLDGDLVHVIPDSLSYEQAALTEPAAVAVY
                     AVRQSSLKAGDTAVVFGLGPIGLLIVEALRAAGASKIYAVELSPERQAKAEELGAIVV
                     RPEEGETAVEAIHRLTNGGVDVSYEVTGVPVVLGQALAAVHKAGECMVVSIWEREANI
                     NPNEFAIQEKTLKGIIAYRHIFPKVLELMEQGYFSAEKLVTKKIKLENIVEEGFIELT
                     QDKSQIKILVEPE"
     gene            29648..30208
                     /gene="efp"
                     /locus_tag="VWZ30_000463"
     CDS             29648..30208
                     /gene="efp"
                     /locus_tag="VWZ30_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU9334265.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            30230..30619
                     /locus_tag="VWZ30_000464"
     CDS             30230..30619
                     /locus_tag="VWZ30_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9334266.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            30612..31034
                     /gene="nusB"
                     /locus_tag="VWZ30_000465"
     CDS             30612..31034
                     /gene="nusB"
                     /locus_tag="VWZ30_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU9334267.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVAEEQ"
     gene            complement(31083..31310)
                     /locus_tag="VWZ30_000466"
     CDS             complement(31083..31310)
                     /locus_tag="VWZ30_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9334268.1"
                     /translation="MLSGLIFGLSHLILSHRDPISLLYYSLIGFFFAFVYRSTDNLRL
                     TILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(31332..31723)
                     /locus_tag="VWZ30_000467"
                     /pseudo
     CDS             complement(31332..31723)
                     /locus_tag="VWZ30_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835936.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(31829..32014)
                     /gene="briC"
                     /locus_tag="VWZ30_000468"
     CDS             complement(31829..32014)
                     /gene="briC"
                     /locus_tag="VWZ30_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEU9334269.1"
                     /translation="MTGTETFTVISTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            complement(32173..32940)
                     /locus_tag="VWZ30_000469"
     CDS             complement(32173..32940)
                     /locus_tag="VWZ30_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU9334270.1"
                     /translation="MNIAKIVREAREQSHLTTLDFATGIFDDFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLRVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGSRAMEAAELMKITSHELLEMDVVDKVISETGLSS
                     KELIKNVKKELQTELARLSQKSLEELLEERYQRFRKY"
     gene            complement(32937..33803)
                     /locus_tag="VWZ30_000470"
     CDS             complement(32937..33803)
                     /locus_tag="VWZ30_000470"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU9334271.1"
                     /translation="MALFSKKDKYIRINPNRSIREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLVLTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     TMREKTGLDEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEQL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(33840..35207)
                     /gene="accC"
                     /locus_tag="VWZ30_000471"
     CDS             complement(33840..35207)
                     /gene="accC"
                     /locus_tag="VWZ30_000471"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU9334272.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKTEDLVFAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAESVGYENAGTIEF
                     LLDEASGNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQIRIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(35219..35641)
                     /gene="fabZ"
                     /locus_tag="VWZ30_000472"
     CDS             complement(35219..35641)
                     /gene="fabZ"
                     /locus_tag="VWZ30_000472"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU9334273.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            complement(35638..36123)
                     /locus_tag="VWZ30_000473"
     CDS             complement(35638..36123)
                     /locus_tag="VWZ30_000473"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU9334274.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(36126..37361)
                     /gene="fabF"
                     /locus_tag="VWZ30_000474"
     CDS             complement(36126..37361)
                     /gene="fabF"
                     /locus_tag="VWZ30_000474"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU9334275.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(37383..38114)
                     /gene="fabG"
                     /locus_tag="VWZ30_000475"
     CDS             complement(37383..38114)
                     /gene="fabG"
                     /locus_tag="VWZ30_000475"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU9334276.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGVISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(38148..39068)
                     /gene="fabD"
                     /locus_tag="VWZ30_000476"
     CDS             complement(38148..39068)
                     /gene="fabD"
                     /locus_tag="VWZ30_000476"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU9334277.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVSSGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(39061..40035)
                     /gene="fabK"
                     /locus_tag="VWZ30_000477"
     CDS             complement(39061..40035)
                     /gene="fabK"
                     /locus_tag="VWZ30_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEU9334278.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            complement(40154..40378)
                     /locus_tag="VWZ30_000478"
     CDS             complement(40154..40378)
                     /locus_tag="VWZ30_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9334279.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(40438..41412)
                     /locus_tag="VWZ30_000479"
     CDS             complement(40438..41412)
                     /locus_tag="VWZ30_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU9334280.1"
                     /translation="MAFSKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGIIGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(41412..41846)
                     /locus_tag="VWZ30_000480"
     CDS             complement(41412..41846)
                     /locus_tag="VWZ30_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9334281.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
BASE COUNT        12615 a         9356 c         7681 g        12331 t
ORIGIN      
        1 agcaaactag gaagctagct gcaggctgct caaaacactg ttttgaggtt gtagatagaa
       61 ctgacgaagt cagtaacatc tatacggtaa ggtgaagctg acgtggtttg aatttaattt
      121 tcgaagagta ttagttaaat cttgcttaat atatctacac catactattt tacatatagt
      181 atttagcacg acataatact gaagtctctc tccaaaaaac ggttatgcct ctttgaatag
      241 gaagttctca caagaaaagc atctccacgt ttcaacttca tatggctcaa aaacaatcaa
      301 ttgatgctaa aacctgtacc tagatgtttc ggttcataaa accatgaaac tgtaaaaatg
      361 gatgaaattg atagcgggta gtcagatcaa gagacatcat aactctaaaa agtcacaata
      421 tagtagattg aagttgaaat agtacacttt ggtttctaaa acattattat aagttagttt
      481 aaattcctta atcaatttgt acatttttat ttcatttcac tatagataag ttcatcctca
      541 gaaaaatatc attctaattg ttgaaatgga tatataaaca ggggtattaa cattaaatgc
      601 tcatgaaaca acgaatacag gtatcaaaac tgtaacaaaa caagtcccta aatttactaa
      661 agacactgct caactttaca cctgtaaatg gttgttgtat aataaagtta caaagatgta
      721 cgaccacacc gttgtaaatc atagtgttcg cgaatatatt actgatagca tttctacaaa
      781 tacaattgaa gaggctagat agggtttaaa cgaaatatgt cagtgtttta gcattcctag
      841 ccttcatatc atttaaagaa ttgtatagac aaaatttttc ccaatacaga cactcgtaac
      901 aactgcttca tttttctacc aacatattta ggaacaggat aagatacaag agaattaatc
      961 catagatcag ttctatacca atctaagata aataagctaa aaaacaatta ataataagca
     1021 aatagattcc aaattttctc tatctgctca ttttaataaa caatactagt gtaactatcc
     1081 ttccagtcag aagcttgtca aaccacatcg aaaatttttc taaaatttat ctcgttaggc
     1141 aatcaagcaa aaactcgacg atagtacaaa cattatcata caggattgac ttcctaaatt
     1201 atatgcttta gtaaggtttt cggataagaa aaaaggttca ttttacatct ctaaacactc
     1261 ttttctaaga tgaaaaacag aatttttcga ttgtgattta aagcaacaag aagattttca
     1321 gtatcatcct atagatacga gctaattaag aaaaactaca tttttgaata taaactacaa
     1381 taagataaac taaatttcat aggaggaaga caatggattg gtacgattat atgatacagg
     1441 catccaaaca atcacaattc aacgcaagcc attggtttcg ctatttgcga aaagttattt
     1501 ttgaagacta ttcttattta acaaaccaag atgtaaaaaa gttgctagac tccaaagaac
     1561 taacccgttt tcaaaaaatt agcttgaagt atgcctttca agagcatact ccaactcata
     1621 aatatgtgat ttcattaaat aaacctgcta agttaaccaa tgttcaaaaa ttgatggaga
     1681 aatacaaaca tggataaaat gaaaccggtc ttccaagccc taaataagga attaattcag
     1741 gaaaatctga ctttaacaat tatctgtgtc ggtggttatg tcttagaata tcatggttta
     1801 cctgccacac aagatgttga tgctttttat gaccaaaatc agaaaataaa tgaaattatt
     1861 gctcgtgtag gaaaacagtt taatcttaac acccacgaag agttatggct caacaatcat
     1921 gttgcaaagc aaatatgaac aaacaacctc ccttaagttt atgcgaatcc ttgtagtcat
     1981 ttgagaatct gaccgtttta gtagtcccta ttgagtatgt gttaggaatg aagatgatga
     2041 gtatcagaga gcaggatttg caagatattg gtgcaattat taaatacaaa aactttcatt
     2101 caccattcga tgcctttaaa tatttaaaag acatgggatt tgatactatt gacctctctg
     2161 ttctattaga aggttttagt tatgcttatg gtatggattg gttagaaaaa ttttttaaag
     2221 aaaatcaaga taaacttcgc gaattttatt aacagtatca aacaagatga ttgtcaatta
     2281 gacagtcgtc ttgtttttat gatgaaatga aataagaata ggacaaatcg atcaggacag
     2341 tcaaatcgat ttctaagaat gttttagaag taaaggtgta ctattctagt ttcaatctat
     2401 tatatttcag attcggtcca ctattccttc ccaaaaacat tgcatattgc gccgaaggaa
     2461 ttgctcacac tatcccaagc acaaacccct cagtcgattg accgagggtt cctttttatt
     2521 attcaagaac ttgattagct caatgcatcc aaggcatcat ccattgaaag aacttcgtgg
     2581 aagacacgtt gtgtcaattc agttttttgt tctggagtga ggtatttagt gtttacacag
     2641 tatccagaga tacgtacgat aacgtcttcg cctgacatga tcttttcgta aacatcgttc
     2701 aagtccataa cgttcaagtt aacgtgttgt ccaccgtttt caaagtaacc atcaaggatt
     2761 gttaccaagt tatcaacttg ttcatcacga gtcttaccaa gagcgcgagg tgatacttgt
     2821 gtagtcaatg agataccgtc agctgcataa ctaaagtcaa ggctagaaag tgagttcaag
     2881 ttttgcaacc aaccaccttt agctttgtta gatgggttag cacctggtga gaagaattca
     2941 agtttagaca agttcacaga accatcttcg ttgaggtata cacctttgtg aactggtgag
     3001 ttaccagttt gtttagagta agcaacgtta gatgtgattg tcaaaagtga tactgtagct
     3061 tctgcgtctt tgtatagttt gtggctacgt agacgagttg tgtaagcttc gatcaaccat
     3121 tctgccaatt cgtttgaacg tgggtcatct tcaccccagc gtgggtagtc accgattgtt
     3181 tcgtaatcgt agatgtagcc atcttcatca cggattggtt taactgtagc gtatttaatc
     3241 gctgacaatg tatcaacagt gttagcaaat ccacaaatac cgaatcccat gttggcacgt
     3301 tgtttagttg gcaagaaggc catttgaaca gcttcgtagt tatacttatc agtcatgtag
     3361 tggatgatgt tcaaggcatc tacgtaagtg tcagtcaacc agtcaagaga tttttcaaag
     3421 ttcgctttaa ctgattcaaa ttcaagaact tcgtcacgaa taggatcgat gtcaaatact
     3481 ttgtagtctt tgtgaacatc gtcgtaacca ccattcaaac cagtaagaag ggctttgaga
     3541 acgtttacac gagcaccgaa gtactggatg ttgtggcgtt gttcttcatt ttctggatca
     3601 agtggagaca cacagcatga gatacagctc atttcaccat atccgtcttt agccattgtt
     3661 gttacacctt cgtattggat agaagagtgt ttgtggctca tgtgcataca gtagcgacgg
     3721 aagttgtatg gcaatttgtc agtccaaaga actgtcaagt ttggctctgg agagttaccg
     3781 atgttgtcaa gagtgttcaa gaaacggtag tccatcttag taacacggtg acgaccgtcg
     3841 ttacccatac cagccataga agttgtgatg aatgttgggt cacctgagta caattggtca
     3901 taagcttttg tacgagcaaa tttaactgta cgaagtttca taacgaaatc atcaacgaat
     3961 tcttggattt ctgattcagt aaatgtacca cgagcaaggt cacgttctgc aaagatgtcc
     4021 aatacgattg gtacacgacc tagagatgta gcagcaccgt taatcacacg gcagacagcc
     4081 atgaaagcaa tgttaaccca ttggattgct tctttcacgt tcatcgctgg tttgcgaaca
     4141 tcaaccccgt aaaggtcacc caggcgaaca acttgttgca atgcttggta ttgaaggttt
     4201 acttcttcac gaagacggat tgtttcttca tcgatttctt tgattgcatt ccagtcattt
     4261 actttttctt gcatcaagta gtctgcaccg taaagagcaa gacgtgcgta aacaccgatg
     4321 atacgtccgc gtgagtatgc atctggaaga ccagttacag tgtgtgcgtg acgagcgcga
     4381 cgaatatttg aagtgtaggc acggaaaata ccgtcgttaa ctgttgttac atatttagtg
     4441 aagatttcgt gaacagctgg gtctggttcg tatccatttt cttttaaagt agtttcagcc
     4501 atacggatac caccttttgg catgaagttc aatttgaaga gttcatcgtt ttggataccg
     4561 aagataactt cattttcttt gtcgataaat ccagcaggga tatcagcgat agatgttgga
     4621 cgagtgtcca ttgggaaacg agtttcttcg tagtgtgctt tagtttcttc tacaattttc
     4681 ttgatgtgaa gtgaacgctc tgttgatcct gcaaggaagc tttcgtctcc atcataaggt
     4741 gtgtagttag cttgtacaaa gcgtgataca cttgcttttt ctttccaatc tacgcctttg
     4801 aagccttccc aagctttgtc aaaaatatct tgtgcttcaa caactgtctt aacaaccata
     4861 ttaatgtcct cttttttcta gtaacagtca tctgttacat tcatgagaca agtataccat
     4921 atagtaaccg ttttcaacaa gtgaaaaatc cctattttta cactttcttt tctaaaacag
     4981 tctatatttt atttcaaact gtattatatt tttgaaaaaa taaagtcctt ttttcttttt
     5041 ttcagaaaaa agggtataat aaaagaaaat aagcagtaac actcaatgga aatcgaaaag
     5101 caaactagga agctagccgc agattgctca aaacactgtt ttgaggttgc agatagagct
     5161 gacgtggttt gaagagattt tcgaagagta taaaaaggtg ctaggcatgt tgatttttcc
     5221 tttgttaaat gatttgtcaa gaaaaatcat ccatattgac atggatgcct tttttgctgc
     5281 agtggaaatc agggataatc ctaaactcag aggaaaacct gtcattattg gaagcgaccc
     5341 tcggcaaaca ggtggacggg gagtcgtttc tacctgtagt tatgaggcaa gagcttttgg
     5401 tgtccattct gccatgagtt ccaaggaagc ttatgaacgt tgtccccagg ctgtctttat
     5461 ctcagggaat tatgagaaat acaagtctgt gggactccag attcgagcta tttttaagcg
     5521 ctatacagat ttgattgaac tcatgagcat tgacgaagcc tatttggatg tgacagaaaa
     5581 taaactcggt atcaagtcag cggtcaaaat tgctcgcctc attcaaaaag atatctggca
     5641 agaactccat ctaactgctt ccgcaggcgt ttcttacaac aaattcttag ctaaaatggc
     5701 gagtgattat caaaagccac atggtttgac agtgattcta cctgaacagg ctgaggattt
     5761 tctcaaacaa atggatattt ccaaatttca tggagtagga aaaaagacag tagaacgtct
     5821 tcatcaaatg ggcgttttta ctggtgctga tttacttgaa gttcctgagg taaccctaat
     5881 agaccgtttt ggtagactag gctatgatct gtatcgaaag gctcgtggca ttcacaactc
     5941 tccagtcaaa tccaatcgca tccgtaaatc aatcggcaag gagaaaacct acgggaagat
     6001 tctccgtgcc gaggaagata tcaaaaaaga gctgactctt ctatcagaaa aagtcgttct
     6061 caatctacat caacaagaaa aagctggaaa aattgtcatt ttgaaaatcc gctacgagga
     6121 cttttcaact cttaccaaac gaaaaagtct tgctcaaaaa acacaagatg ctagtcagat
     6181 aagccaaata gccctgcaac tctatgaaga attaagtgag aaagaaagag gtgtccgcct
     6241 attggggatt accatgactg gattttaaag ctcaatgaaa atcaaagagc aaactaggaa
     6301 gctagccgca ggctgctcaa aacactgttt tgaggttgca gataaagctg acgcggtttg
     6361 aagagatttt ctaagagtat aaaaccttga agagttgccc cttcaaggtt tttcttatac
     6421 aaataaacgg acaaaactgt aaagtagcaa aacactacca agcacgatac ggtatttacc
     6481 aaaaagggtg aagtcgtgtt ttttcacata gctggtcaag aagcgaatag ccaccatgct
     6541 gaccgcaaaa gctactccca tcgcgaccaa gagcaaaaac aattgcccaa agctcaagag
     6601 ttctccggct ttcacaaatt tgaaaatctt taaggcacta gctccaaaca taacaggaat
     6661 cccaagatag aaggtaaatt ctgtcacaac tgaacgactg gttccattta acaaaccacc
     6721 gacaatcgtt gcacctgaac ggctagtccc tggtaaaaga gcaagaactt ggaagagtcc
     6781 gatatagaaa gcggtcgtat aaggaagctt gtccaactct gttacacttg gctcgatagc
     6841 acgcgcttta ttgcgctttt ccaaatagat gaaggcaacc ccgtagataa tcaacatgag
     6901 agcaactgaa accatgttat ggaagtgggt atcaaaccaa tcatcaaatt taaagacacc
     6961 aagtaaaggt aaagtagcaa tcaagacctt caaccatagt ctccaagtct tacgaacttc
     7021 ctgtttgtcc ttagtcggtt taaaaggatt gagcttgtta aaataaatca ccataactgc
     7081 taagatagca ccaagctgaa tcacgacatt aaacatggac ataaaggctt cattttgatt
     7141 ttggtattgg ataaactcct ctgctaaaat caagtgacct gtactggaaa tcggcaacca
     7201 ttccgtaatt ccttcaacaa tcccgaagaa gatagatttt aaaatttcaa taagatacat
     7261 agattactcc ctatattata tttagcgcag ttagctactg ccaaagccca agtggtatac
     7321 ttggaataag ccactgtgga ttagttcatt tcctccaatc gcttgacgat ttccgaaagg
     7381 ctctaaccac agcttttgtt tcaattggta atgagtttga taacgcatag acgttttatc
     7441 tcgtctaagg taacaaggca aaaagttcgt ggataaccaa gtagacttga aatactatat
     7501 ctggaggtac tcccatgctt tatgtgggca ttgatgtcgc taaaaataaa cacgatgtta
     7561 cagccttgaa tgttccagga aaaactgttc ttaaaccact cactttttca aataataaag
     7621 ctggttttga actcttagat ctgtctcttc gacagctcaa ccaagactgt ctcatcgctc
     7681 ttgaagatac tggacattat gcctttaatc tactgaactt cctgcatgaa caaggatatg
     7741 aagtttacac ctacactcct ttgctcatca aggaatttgc caaatcatta tcacttcgca
     7801 agactaaaac ggataagaaa gacgctcatg gcatcgctct taaacttctt tctgacccca
     7861 atcgtgaaca atttcaacac gataatcggc aagtagaact aaaaatactg gctagacata
     7921 ttcatcgtct caagaaaaaa cagtctgatt ggaaagtaca atacactcgt tgtcttgata
     7981 tcatctttcc tgagttggat aaaatcgttg gaaagcattc agaatatacc taccaactct
     8041 tgacacgcta ccctaatcct cagaaaagga ttgaggcagg atttgataag ctgatagaaa
     8101 ttaagcgatt gaccgcttct aaaattcagg atatcctctc agtcgcacct cgttctatcg
     8161 gaacaacatc tcctgctcgt gaattcgaaa tcatcaaaca ttacaagagg ctcattgaca
     8221 aggtggaaaa atgtgtcaat gacttgatgg ctgagttcaa ctcggtcatc acgacggtca
     8281 ctgggattga taatcgttta ggggcggtca ttttagccga gattcgaaat attcatgcct
     8341 ttgataatcc tgctcaatta caagctttcg ctggactgga ttcttctatt tatcagtcag
     8401 gtcagattga tttagctgga agaatgatca aacggggttc ccctcatctg cggtaggcac
     8461 tcatacaagc tgccaaagca tgcgctcgct tttcacctgc ttttaaggcc tatcttaaga
     8521 ctaagttaga ataaggaaaa cattacaatg tagccatcat ccaccttgca aaaaaactta
     8581 tccgaaccct gttttatatc ctaaaaaaga gctgccattt gacgaacaaa aagtgatgta
     8641 atttctgaaa ttaacacagg attacctttg aaaaaatgga ctttttcaag ggctttttgc
     8701 cgtgctctaa aatcatcgcc aaatcatcaa attgaaataa gaaatagttt agataaaact
     8761 attgactttt cttatagttt gtcttttttc tatcttccat tatatcatac tttttcagtc
     8821 tatggtagct ctctttaaaa ggcaccgata ctgccgccac ctccgcctcc agagaagcca
     8881 ccaccagaac ttccacttcc agaagataca gagtaggtgc ttgctgtatt tgcgacacta
     8941 gcataatgac tcatttgtgc tgttgaatga taaaacatac tgtgccagcc ataagctaca
     9001 tagaaattga tatctggatt ttccacttga atctgatgaa ccttcatcaa atgactaacc
     9061 ttgtccgcat agccaaataa ggtcgcatag accaagaggc gattccagac cacaatactt
     9121 tccagttcag cctgatccaa tcgtgcaatc tcacgcaaca tattttcaaa actggtccag
     9181 agatagtaga cctcagctcc cgcttcattt agaacaccat cacgattatc tagttgaagc
     9241 ttccaataat agagaacaga caaaactaac cctagaaaac caagtattgg caaggggagg
     9301 taaagatagc catgaacgtc taaactgtac aagaacaaac caaatccgat aaataggggc
     9361 aagatagtca agacacccat acccacttgc aaagcctttt ccccaccagt taaaggacga
     9421 tagtaatctg ggagtcccca gaagacaact cgctttctca ctccttcttg catctctttt
     9481 aatactgctt cgaaagaaga tttgagctga atccctcttg cttgaatccg tttttcatca
     9541 gaaactttgg ctctacgata aagactatca gataccttgt aatccgcaaa caaattggaa
     9601 agagtttctt cttttttacc tgaaaaagct agatgtaggc agtctttctc aaagcttgac
     9661 aaaccatctt cttttactag cctcaaacca actgcatctc cttctgaaat aatagaaaca
     9721 ttcccacggt ccatcacatc tagcaaggta gcttgaataa gttggtcaaa agtgaatttg
     9781 ccagccccct tgactaaggg actcacttcc tccaaggagg tcgagtagac agcctctgat
     9841 aaaaccatag gctctaattc cattggcggt tcgtagagac gatgattttt agcatatttg
     9901 attgaaggag cggtttttct tctatagata aaatagaaac agatgctcaa taacaaggag
     9961 atggaaagga tcgaagggaa gatccaagta agtagttgtt tactttgctc tttttcttta
    10021 acaatcgaat cctctatctt attaaactct tctaaacgat tccctttcaa gccctgatcc
    10081 ctagcgctag caaaatcagt tcgaggccag taggcatgca actcagctcc acgcttagcc
    10141 ggaagattgt ctaaacgaat agtataatca aggttactct tttcaaccgt tctctctcta
    10201 aaaagtttcc ctgtatggaa aaagagtttc tcagccccct tgtctcccct tacatgaaat
    10261 tcaaactttt caatagactc tgaactatct gtcagaggtt gccaatttaa ttcagcgata
    10321 tcatcataaa ggaaaagtaa attttttaag ttccagacga gatcaacttc aactatgtcg
    10381 ccctcctgac ctggattata gactctcaca gtataaccat ccgcttcttc tgttacttcg
    10441 ctagtcacat ctgctagttc tgcaccgttt ttcgcggcct gaacctttgg atgagggtca
    10501 atgtcaaacc cgctaggcat cttgccagca cgtccaagtc ccacgatttg gcccttaaag
    10561 tcctcctcaa actggtaaac tatcttctgt ctaaactctg ccgtattatc tgcatgaata
    10621 tacaaatccc cttgataaga gtttatcttg aaatcaatgg caaaaacaga gagtggaaga
    10681 aggcaaaaca agcctaacac cagtaagaaa aaagtttttt tcatcaacta agcccccttt
    10741 ttatctttct tatcattata gcattttttc gtaagcaaag gcttacttat gttgaaaaat
    10801 tacgcatttt tcgataaaat aggagacagt tattttttaa tagattagaa ataggagaaa
    10861 ttatgagaaa aatatactta tctattttca caagtctctt gctgatgcta ggacttgtca
    10921 atgttgctca agccgatgaa tatttacgca tcgggatgga agcagcatat gctcccttta
    10981 actggaccca ggatgatgat agcaacggag ctgtcaaaat cgatgggacc aatcagtatg
    11041 ccaacggata cgatgttcaa atcgccaaga aaatcgctaa ggacttaggt aaagaacctt
    11101 tggttgttaa aaccaagtgg gaaggtctag tccctgccct tacttctggt aagattgaca
    11161 tgattatcgc aggtatgagt ccaactgcag aacgcaaaca agaaattgcc ttttcgagca
    11221 gttactacac tagcgaacca gttctactgg tcaaaaaaga ttctgcctat gcaagtgcta
    11281 aatctttgga tgactttaac ggtgcaaaaa tcacttctca acaaggggtc tacctctacg
    11341 acttgattgc acaaatccca ggtgctaaaa aagaaacagc catgggagac ttcgctcaaa
    11401 tgcgccaagc tcttgaggct ggtgtcattg atgcttatgt ttctgaacgt ccagaagcac
    11461 tgactgctga agctgcgaac tctaagttca agatgattca agtagaacct ggtttcaaaa
    11521 ctggggaaga agatacagct atcgctatcg ggcttcgtaa aaatgacaat cgtattagcc
    11581 aaatcaatgc cagcattgaa accatttcaa aggacgacca agttgccctg atggatcgta
    11641 tgatcaagga acaacctgcc gaagctacaa caactgaaga gactagcagt agtttcttta
    11701 gccaagttgc taaaattctt tctgaaaact ggcaacaact cttgcgtggt gctggtatca
    11761 ctcttttaat ctctatcgtc ggaaccatca caggtctcat tattggactt gctattggtg
    11821 ttttccgtac tgctcctctc tctgaaaaca aagtcattta cagcctacaa aaactagtcg
    11881 gctggatcct caacgtctac attgaaattt tccgtggtac gccaatgatt gttcaatcga
    11941 tggttatcta ctatggaact gcccaagctt tcggtatcaa ccttgaccgt acactagctg
    12001 ctatcttcat cgtttcaatc aataccggtg cctacatgac tgaaatcgtc cgtggtggta
    12061 tcctggcagt tgacaaggga caatttgaag ctgcgactgc tcttggtatg actcataacc
    12121 aaaccatggg taagattgtc ctacctcagg ttgtccgcaa tatcctacca gcaactggta
    12181 atgaatttgt catcaatatc aaagatacat ctgtattgaa cgttatctct gttgtcgagc
    12241 tttatttctc aggaaatacc gtggcgacgc aaacctatca atacttccag acatttacaa
    12301 tcatcgccgt gatttacttt gtcctcacct tcaccgtaac acgtatccta cactttatcg
    12361 agcgcagaat ggacatggat acctatacta caggtgctaa ccaaatgcaa acggaggatt
    12421 tgaaataatg acacaagtaa tccttgaaat taaacacctc aaaaaatcct atggacaaaa
    12481 cgaagtgcta aaagacattt ctctcactgt ccacaaggga gaagttatct ctatcatcgg
    12541 aagctctgga agtgggaaat cgaccttcct acgctccatt aacctacttg aaacgccaac
    12601 tgatggacaa atcctttatc atggacaaaa cgtcctcgaa aaaggctatg acctcacgca
    12661 ataccgtgaa aagttaggga tggttttcca atcctttaac ctctttgaaa atctcaacgt
    12721 tcttgaaaac acaatcgtcg ctcagacaac tgtccttaaa cgcgaacgca cagaagctga
    12781 agaaattgcc aaagaaaacc tggaaaaggt cggcatggga gaacgctact ggcaagccaa
    12841 accaaaacaa ctctcaggtg gtcaaaaaca acgtgtggcc atcgctcgtg ccctctccat
    12901 gaatccggac gctattctct ttgatgaacc aacatcagct ctcgatccag aaatggttgg
    12961 agaagtcctc aaaatcatgc aggacctggc tcaggaaggc ttgaccatga ttgtcgtaac
    13021 ccatgaaatg gaatttgccc gtgatgtctc tcaccgtgtt atctttatgg ataagggcgt
    13081 gatcgctgaa gaaggtaaac cagaagacct cttcaccaat cctaaagaag accgaacaaa
    13141 agagttcctt caacgctatc tcaaataaaa agaaaaggct gcatcaaccg tgcagtcttt
    13201 ttgctgtcct caaaatgaaa aggcagattc tctatctcta ctacttgaag gtcaagaatc
    13261 cgccgttatc caaagattaa tcttcaaatt tttcataatt tttttcatag aaatcaatta
    13321 ggcctagggc tgtttcaaat gaaatatttt ttacttttgc acgaccctgc gccagagcaa
    13381 tgatagacat ttcacgcgca ttcgtttctt tagagatacg gtagcctgtg attttcttat
    13441 cacgaaccca gccaacaact gattctactt tttcaaagtt tgacttagcc atgtttttct
    13501 cctattttct tctttataat atttaattgt atacgttttt actacttttg tcaataaaaa
    13561 aaacatatat tcaacgaaaa taattagttc agaattttct tacttccttt ttaaacccga
    13621 taatatatag gttttgctag cctctacacc ataaatattt gctaataaaa atactgtatt
    13681 attaccctct taaggtgcga aattattcaa gttcttaaaa aatgcttctc tatttccgta
    13741 agatagctta tttcttcgat ttaagaactg aagatgatag ttctaacctt tagaaatcca
    13801 agccttcact agctcaaaaa ctcctatctt tcttcctcaa taaatcatct gagagatagg
    13861 tattccttat atccttcaaa ttcatcactc ctttggatat tgaggtgcta gcctagaagc
    13921 agacacccga ttatctaaac catccttaat agagatgaca agacagataa gctcagttaa
    13981 ttacttatat atctttttaa tatggtatat ttgtcaacaa aaagaaatca aatcatacct
    14041 tatactcaat gaaaatcaaa gagcaaacta ggaagctagc cgcaagttgc tcaaaacact
    14101 gttttgaggt tgcagataga gctaacgtgg tttgaagaga ttttcgaaga gtattaaaga
    14161 cagtgtgact atgcttgtca atagatagga aatcaaaatg aaggatggaa atctgagcca
    14221 aacatcataa atgctataat gaacagaagt aagaattgca cgataagctt cctaaaacca
    14281 taggaatcgc tccctttctt catcttcatt atagcaccta tacatcagtt gtgcaaataa
    14341 tatgatattt ttttattagt cctcagacaa gcatattata aagcgtttca ttaccattta
    14401 agttaatata agctggatca aaaccttcca ttctacggat caaacctgca taatcatgct
    14461 tatctgccaa agcgatccca atcaatactg ggcctgttcc cttgctagct cgtttgatat
    14521 actcaaaacg tgtgatatcg tcatttggac ccagaatatc atttacaaac tcacgcaaag
    14581 cacctggacg ctgtggaaaa ttgaccacaa agtaatgctt gatgccatca taaatcaagg
    14641 cacgctcttc catttctggc atacggttga tatcattatt tcctccagaa atgatacaac
    14701 aaatggtttt ccccttaata tattcagcca aaacctctaa agaggcgata ctagccgctc
    14761 cagcaggttc tgcgacaatc ccttgcttag agtagaggtc aatcaaagtt tcagaaatca
    14821 gcccctcatc aacacctact aaagtttgaa catgttgacg agttgcctca taggtcaact
    14881 gacctacctt ttgcacagca atcccatccg caaatttatc aatttccttg agtttgactg
    14941 gacctccagc ctcaaaggca gctttcatgg aacgcgctcc attcgcctct actccgataa
    15001 tctcaatctc tggacttgtt tccttgatat aggtagaaat cccggcaatg agaccgccac
    15061 caccaacagg aaccaagaca gcatcaaaat caatcgattc ttttcgagct tcttctaaaa
    15121 tctcataagc aactgttcct tgacctgctt gaacatgagc atcatcaaaa ggatcaataa
    15181 aggtacgatt ctcagagact gtaaattctt gagctgcttt ggctgaggca tcaaaggtat
    15241 ctccaactag tttaatagtt acaaaatccc caccaaaaaa gcgaacttga ccaatttttt
    15301 gttgtggcgt agtaatgggc ataaagatag tagcaggaat tttcatctca ttacaagtat
    15361 aggcaactcc ctgcgcatga tttcccgcag aggcacagac taccccacgc tcacgctctt
    15421 ccttgctgag ctgagaaata gcataatagg caccacgaat tttaaaagag cgaacacgtt
    15481 gagcattttc ctttttcaaa taaatcttag caccatactt ctccgataaa taatgatcgt
    15541 aatccagtgg agtattcaca accacaccgt tcaagacctt gtgagccttg attatatctt
    15601 ttgaacttaa catataaaac ctctttttct ataaaaaaca gtcactaatg actgtttttt
    15661 agttattcat ctccgaaaac ttcagtaaca ctttcatggt atttatgagc catacgaatc
    15721 gaaaactctt taggaagttc ataacgattt gtggaaatcc aatcatataa ccattctacc
    15781 aaactttctt cactatcatt acgaaagttt tcgcagtcca gtcatttaaa cccgattctt
    15841 tcaatccaga tgcaataatt tgctctattt gatataaacc gccatctatc gtctcattta
    15901 ttctgcgaag aggaccttca atataggata aagcttctcc gaatctaaca gctgatttag
    15961 gtcctaatga tttaccaact atatttgtta atgtttttta tttctcaaaa tccatttcgc
    16021 tatataacga gcagatttag tcgcaccagc tctctcaaac ggagaatgaa tttcaagacc
    16081 ttcttctcta gatatttgtg ccaatttgaa gaattgttca aagttcatat gcaacctacc
    16141 atcttcaaaa ttagcatcat cataaatgtt aattacagca ttttcatcat acgaccaatc
    16201 tgctttattt tggtctgcta gcactattac cgacggtgta attacattat acaactagaa
    16261 ccaataataa cgatgcaatt tttctcataa tctttttcat aaaatcatct ccagcgctat
    16321 atatttctaa ttagttatag attttgaatg catcatcgtc gtttttacca acgaatggca
    16381 ttgctttacg caattctgca ccaacttttt caatttcaag gttagctgct tgttcacggt
    16441 aagcagtcaa ttttggacgt ccagctttat agtcatttac aaagtcattt gcaaatttac
    16501 cattttggat gtctgccaag acagccttca tattttcttt aacttgttca gtgattacac
    16561 gtagacctga tacatagtca ccgtattcag cagtgtttga aatagattga cgcattttct
    16621 tgaatccacc ttcgtagatc aagtcaacga tcaatttcat ttcgtgaaga acttcaaagt
    16681 aagccaattc tggagcgtaa cctgcttctg tcaagacttc gaaacctgct tcgataaggg
    16741 cagtcaaacc accacaaagt acagcttgtt caccaaacaa atcttcttca gtttcttctt
    16801 tgtaagttgt ttcaagaaga cctacacgag ccgctccaac acctttacac cagtccatag
    16861 caatgttttt agcatttcct gttgcatctt gatatactgc ataaagagct ggaacaccaa
    16921 atccttcttc gtaagtacga cgtaccaagt gtcctggtcc tttaggagca cacatgaaga
    16981 catctacatc cgcaggaact ttgataaatt caaagtggat gttgaaacca tgggcaaatc
    17041 caactgcgtt tccagcttcc aagtttggag cgatttctgc ttcgtacaat tcttgttgaa
    17101 tttcgtctgg cgccaagatc atgataacat cagccaactt agtagcttct gctactatgt
    17161 aagtatcaaa tccatcttct tttgctttat caaaagattt acctggacgt acaccgataa
    17221 taacgtcacg acctgaatca cgcaagtttt gagcatgcgc atgcccttgt gaaccataac
    17281 cgataacggc gattttttta ccgtcaagtg ctgctacttt aacatctttt tcatattcca
    17341 tttgaactgt catagctttt ctctcttttc tattatttat tgccttttag gctggtttaa
    17401 taaatttaag ttggattttt aatcgcgggt aaatccagtt gcacccgttc gagcaatatt
    17461 gcgaataccg tatgggcgaa tgactcgcaa tagggcttcg ctcttttctg catttcccgt
    17521 catctgaatg gtaatcgagc ttggcgctac gtctaccact gttgcacgga aaggttgaat
    17581 aatcgctaaa atctcagctc tcttctcagc tggcgctgac atcttaacca aaatcacctc
    17641 gcgctccaaa tgaggcttgt ctgtaatatc tcgaatgcga atcacatcaa tctgacgatt
    17701 gagctgtttg atgatttgct ccacttcatc atgagaagca acatcaataa taatagtgat
    17761 acgcgataca ttcggatctt ctgttgctcc aacagagatg ctttcaatat taacctgacg
    17821 acgagatagg acacctgtaa agcgattgag gactcctgaa cgattttgta gttttgctgt
    17881 taacattcta cgcatggaac tgcaccccca acatctcatg attactctta ccagccggta
    17941 ccattggtaa cacctgttcc ttacgagaaa tatctacctc aattagcata ggaacatcct
    18001 cagtgatgac ttcaaggtct tgagccaagg tctcaggatt gtcaaactta tagtttttaa
    18061 taccataagc ctgcgccatc aattggaaat caggaagggt atcaaagacc gactctgatg
    18121 ttctgccttc atagaaggat tcctgccact ggcgaaccat tccaagtgaa tgattgttca
    18181 gcataaccac cttgattggc accttgtaaa tattcaaaat agccaactcc tggttggtca
    18241 tttggaaacc accatcccca acaaacaaga ctacctcctt atctgggtta gcaattttag
    18301 caccgattgc tgctggaatt ccaaatccca ttgttcccaa accacctgaa gtcactaact
    18361 gacgttcatt ttggtaggga taatactgag ctgtccacat ttggtgttga ccaacgtctg
    18421 ttaccacaat ggcatctcca ttcgtcaatt caccaattcg ttcaataact gcttgcggtt
    18481 gaaccacacg ctctttcttg tcataagaac gaacacgatt cttgtcttta gtgactttct
    18541 caatccactt ttcagtattg ttgtgaactg ttggttctgc tagcaacatt tgcaaggcct
    18601 tcttagcatc tccaactaca ggaatgtctg cactgataat cttgccaatc tcagctgggt
    18661 caatatcaat gtgggcaacc ttagcattct tagcgaaagt cttaggattc cccgtcaaac
    18721 ggtcatcgaa acgagaacca atactaatca taaagtccgc ttccgtcatg gcaatatttg
    18781 ctgcgaatga cccgtgcatg cctcccattc caagaaagag tgggtgactc gttgcaatcg
    18841 ttccttgtcc caaaagactg gttaccactg gaatttgata gcgttctgca aattcattta
    18901 gttccgtagc agcctcagca taactaattc caccaccagc taacaagact ggctttttag
    18961 ccttggacaa ttgcttcaag attttcttga tttgcatatc attcggctca agagtcggct
    19021 gataacttgg taaattcact tctggtgaat aaatgaagtc tgtttctaaa gcagatatgt
    19081 cttttggtag gtcaattaca actggccctg gacggcctgt agttgcgata tggacagctt
    19141 ccgtaatgat acgcggaata tcagctgtct cacgaacttg gtaattgtac ttagtgattg
    19201 gcatggtaat tcccacgatg tctgcctcct gaaaggcatc cttcccaatc cctgctcgcg
    19261 ccacctgacc tgtaaagacc aaaaggggaa cgctatcgct catggcatcc gcaatccctg
    19321 taatggcatt tgttgctcct ggtccactag tgacgacggc aacacccaac tttccagttg
    19381 atttggcata accttcagct tcatgcaaac aaccttgctc atggcgccct agaatgtggc
    19441 gaatgccttt aaaattatat atcgcatcat aaaaaggcaa aaccgcacca ccaggataac
    19501 caaagatggt atcaactcct aaatcacgaa gtgtttccaa aactaggtcc gaccccgtct
    19561 taggagattc taaactgatt ttctccatta ttcccctttc tttcctctta aaaataactt
    19621 gttactatca taccattttt tcaaaatttt tcaagataaa agaagaaatt ttctgaattt
    19681 tctattttaa cgtttattta tgaatgttat ttttgttttt attaaaaaga taccgatttc
    19741 atttactaaa aagaaaaaag aactgatttc tcagtccttc attaatctta ttccacacta
    19801 aataggtatg ggtaaacagg ttgttgacct tggtgaatct cgacttcaac gtcttcgaat
    19861 tcttctacga tttcttgagc gatttcattg gcaagttctt cgcttccgtc ttcaccgaca
    19921 tagaaggtta cgatttcact atcttcatcc aacatatgtt tcaaggtttc agtcaatgtt
    19981 tggtgcatat cagggtttga cacaagaatt tttccatcca ccatacctag attatcgttt
    20041 tcatggattt ctaagccatc gatcgttgta tcacgcacgg ctgttgtgac gcttccgcta
    20101 acgacatcgc taagagcagc tgtcatacgc tcttggtttt cttcaatgga cttgcttgga
    20161 tcaaaggcaa gaagacttgt cataccttga ggaagagtgc gagcctctac cactactgct
    20221 ggttgctcca aaacttctgc cgcagattga gctgccatga agatgttctt gttgtttggc
    20281 aagaagatga tgttacgggc attgacctgt tcaacagcct tgataaagtc ttctgttgaa
    20341 gggttcatgg tttgaccgcc ttcgataaca taatccacgc cttgagaaca gaagatatct
    20401 gctagacctt taccagccac cacagcaatc aaagcatact ctttttcttc agccgacttg
    20461 ataacttgag tagcttcttt ctcaacctgt gcttcgtgtt ggttacgcat attgtcaact
    20521 tttaccttga ccaagctacc atatttgaga ccttcttgca taacaagtcc tggatcttct
    20581 gtatgaacat ggactttgac aatttcatca tcgttaacaa cgaggagaga atccccaagc
    20641 tcatccaagt agttacggaa ttcatcgtag tcaaaatctt tagcataggt tggaccttgc
    20701 ttaagagcta ccatgatttc agtacagtaa ccaaacgtga tgtcctcagt tgctacgtga
    20761 ccagctacag acttatgatg ctctacattg atcatctcac tcatgttggc aggagtcgct
    20821 acaaagtcct cagatgcaat atattcgcca gtaagggctg aaaggaaacc ttcgtagatg
    20881 aagaccaatc cttgaccacc tgagtccaca acgccaactt ctttcaatac tggaagcatg
    20941 tctggtgttt tagctagagc tgttttagca ccttccaagg ctgcgcgcat gacttcaaca
    21001 gtgtcatctg tttgctcagc ttttttctta gcaccgatag cagctccacg agaaactgtc
    21061 aaaatcgttc cttcaacagg tttcatcact gccttatagg caacttccac acctgattgg
    21121 aaggccagag ccaagtcttg acctgttaac tcgtctttat ccttgatagc ttgggaaaat
    21181 ccacggaaaa gctgagacgt aatcactcct gagttcccac gcgcacccat caaaagccct
    21241 ttggcaagaa tgctcgctac ttctccaact gtagaagctg gcttgtctgc aacttcttta
    21301 gcaccatttt caatggtcat tcccatattt gtcccagtat ctccatctgg aactggaaag
    21361 acgtttaatg aattgacata ttcagcttgc ttattcaagc gagttgatgc agcctgcacc
    21421 atttcttgaa ataagctagt agtaattttt gacacggtta ttctcctaca actttgatat
    21481 tttgaatgta gacatttaca gtctgagcag taattccaag ctggttttcc aagctaaagg
    21541 caacacgctc ttgaatgttt tttgacactt cactaatctt tgttccgtag cttaacacgg
    21601 tatatacatc aactgcaata ctgccatctt cggctgcctt tacgacgaca cctttagaat
    21661 aattttcctt acctagcagg gcttggaaat tatctttgag ggcattttta ctagccatac
    21721 cgaccacacc aaaaatctca gttgctgctc cacctacgac ggttgcaatc acttcatctg
    21781 ttagttcgat ttgaccatct tttgtattaa tttttacagt catccttttt acctcaacta
    21841 gttgatactc tattttatca tatttcagcc caagtgtaaa agcagaatga tgtatcagcg
    21901 gatatttact ctatttttca aacgatgtta tacccacaat aaaagaaaaa agaccctaag
    21961 gtctcctttg cttttattat taaacgcgtt caactttacc tgatttcaaa gcacgagctg
    22021 aagcccaaac ttttttaggt ttaccatcga taagaacagt aactttttga aggtttggtt
    22081 ttacggcacg ttttgtttgg ttcatcgcgt gtgaacggtt gtttcctgat acagtcttac
    22141 gacctgtaaa gtaacatact ttagccattg tgttttcctc ctattagatc taatatagcg
    22201 gatgtgctag caccacatac cgtactatgt tatcacactt tcttgttttt tgcaagggaa
    22261 ttggaagatt ttttatttgt gtcttaaatc aggtcttgcg tgacattttc tgctctccac
    22321 atgccatcgt tgattaacag aacaccagaa ttaaaattat gtgtataaaa atcatctcta
    22381 actgcagcta agggtatagc cgtcaagtcc aaatcccaca gctcatctat cgattttctt
    22441 acaacaatat ctgaatccaa atacagtaca cgagactcgc ttacatactt tggaataaaa
    22501 tacctaaaaa agccgcatat gaaagtccct caaaggggag acgataacct ttcagaatat
    22561 tactgtcaat ctaaacattc acaatctcac tattcaaagt ctctagtctt ttttccatca
    22621 attggaacca ttctcgcgga aggtcatcat taaaaacata aaacttaaga ttataatgat
    22681 gaacacaaag agattttatt gttgtttcaa ctttatccat ataagcatta tctgcaccta
    22741 agacaatcgc ttttttctct tctttcactt tttatctcat ttctttttat tcccatcata
    22801 ttattcccat catatgtttc tacgtaacca ttattttcgc ctattcgttc gtaaaactat
    22861 accagtggag attttagatg aagtcccatt acggtttaca atttttacat tacgacacgg
    22921 agttttacaa atcgatttca tttgccaaac gtagttagta aggcagttag ctaggctacc
    22981 tactttctgc tacgacggaa aaatccacaa atcaatacca ttcgtggatt tttcctagtg
    23041 aagcgtttag gtaagccgcc atagcggtta ccgattatta gattacgaca cggagtttgg
    23101 caaatcgatt ctatttgcca aacgtagtta gtgactcagt accaacaatt tggaacttta
    23161 gttccaaatt gttggtactg agtcacatct tctcctctaa ctctacgtct ggatacttgt
    23221 ccgcaaacca gcggagggca aagtcatttt caaagagaaa gactggttgg tcaaaacggt
    23281 ctttggctaa gatattgcga cttgacgaca tccgttcatc caagtcctca ggcttgatcc
    23341 aacgaacggt ctttttaccc attgggttca taactacttc cgcattgtac tcaccttcca
    23401 tgcggtgttt aaagacttca aactggagtt gacctacagc gcctagcatg tactcacctg
    23461 tttggtaatt cttataaagc tgaacggctc cttcttgcac caattgctca atccccttgt
    23521 ggaaggattt ttgcttcata acattcttag cagaaacttt catgaaaatt tcaggagtaa
    23581 aggttggcag tggttcaaat tcaaacttgt ttttaccaac tgtcaaggta tccccaactt
    23641 gataagtacc tgtatcgtaa accccgataa tatcacctgc cacggcattg gtcacattct
    23701 cacgactttc cgccataaac tgagtaacat tagacagttt agccccctta ccagtacgag
    23761 ggagattaac actcatgccg cgctcaaatt cgccagatac gatacggaca aaggcaatac
    23821 ggtcacggtg acgagggtcc atgttggctt ggattttaaa gacaaagcct gagaaatcct
    23881 tgtcataagg atccacaatt tcaccatctg ttttcttgtg accatgtggt tctggagcaa
    23941 acttgaggaa ggtttcaagg aaggtctgca caccaaagtt tgtcagggct gaaccgaaaa
    24001 agacaggcgt caattctcca gccagaatag cttcctctga aaactcattc ccagcttcat
    24061 ttaaaagctc aatgtcatcc ttgacttgat cgtagaaagg attgctacca aagagtttgt
    24121 ccccgtcttc tagactggca aaacgctcat cccctttgta aagctctaaa cgttggttat
    24181 agaggtcata caagccctca aaggctttcc ccatcccgat aggccagttc atagggtagc
    24241 tagcaatgcc caagatttct tccaattctt gcaagagatc caaaggctca cgaccgtcac
    24301 gatccaactt gttcataaag gtaaagactg gaatgccacg atgtttcaca acctcaaaca
    24361 gtttctttgt ttgagcctcg atccccttgg cagagtccac gaccatgacc gcagcatcca
    24421 ccgccatcaa ggtacgatag gtatcttctg agaagtcctc gtgccctggc gtgtctaaga
    24481 tattcacgcg cttgccgtcg tagtcaaatt gcataacaga tgaagtaaca gaaatcccac
    24541 gttgcttctc gatatccatc cagtcagatt tagcaaaagt ccctgttttc ttccctttta
    24601 ccgtaccagc ctcacgaatc tcacccccaa agtagagtaa ctgctcagtg atggttgttt
    24661 tccccgcgtc cgggtgggaa ataatggcaa aggtacgacg tttcttaatt tcttcttgaa
    24721 tattcataag ttctctttct ttgattctct atttttcttg tttcaatagc tgagaatgat
    24781 ttttacattg gattttacca ttcctttcaa cactccatta tatcggattt tagcattttt
    24841 ttcaatttct atttcttttc acttccccct cccttattta taggaaaata tggtaaaata
    24901 gaacagacta aaaatcatca tttcacgaaa ggatgcaaga tgaaaattac gcaagaagag
    24961 gtaacacacg ttgccaatct ttcaaaatta agattctctg aagaagaaac tgctgccttt
    25021 gcgaccacct tgtctaagat tgttgacatg gttgaattgc tgggcgaagt tgacacaact
    25081 ggtgtcgcac ctactacgac tatggctgac cgcaagactg tactccgccc tgatgtggcc
    25141 gaagaaggaa cagaccgtga tcgcttgttt aaaaacgtac ctgaaaaaga caactactat
    25201 atcaaggtgc cagctatcct agacgatgga ggagatgcct aatgactttt aacgataaaa
    25261 ctattgaaga gttgcacaat ctccttgtct ctaaggaaat ttctgcaaca gaattgaccc
    25321 aagcaacact tgaaaatatc aagtctcgtg aggaagccct caattcattt gtcaccatcg
    25381 ctgaggagca agctcttgtt caagctaaag ccattgatga agctggaatt gatgctgaca
    25441 atgtcctttc aggaattcca cttgctgtta aggataacat ctctacagac ggtattctca
    25501 caactgctgc ctcaaaaatg ctctacaact atgagccaat ctttgatgcg acagctgttg
    25561 ccaatgcaaa aaccaagggc atgattgtcg ttggaaagac caacatggac gaatttgcta
    25621 tgggtggttc aggtgaaact tcacactacg gagcaactaa aaacgcttgg gaccacagca
    25681 aggttcctgg tgggtcatca agtggttctg ccgcagctgt agcctcagga caagttcgct
    25741 tgtcacttgg ttctgatact ggtggttcca tccgccaacc tgctgccttc aacggaatcg
    25801 ttggtctcaa accaacctac ggaacagttt cacgtttcgg tctcattgcc tttggtagct
    25861 cattagacca gattggacct tttgctccca ctgttaagga aaatgccctc ttgctcaacg
    25921 ctattgccag cgaagatgct aaagactcta cttctgctcc tgtccgcatc gccgacttta
    25981 cttcaaaaat cggccaagac atcaagggta tgaaaatcgc tttgcctaag gaataccttg
    26041 gtgaaggaat taacccagag gttaaggaaa ccattctaaa tgccgctaaa cactttgaaa
    26101 aacttggtgc tattgtcgaa gaagtcagcc ttcctcactc taaatacgga gttgccgtat
    26161 actacatcat cgcttcatca gaagcttcat caaacttgca acgctttgac ggtatccgtt
    26221 acggctatcg cgcagaagat gcaaccaacc ttgatgaaat ctatgtaaac agccgaagcc
    26281 aaggttttgg tgaagaggta aaacgtcgta tcatgctggg tactttcagt ctttcatcag
    26341 gttactatga tgcctactac aaaaaggctg gtcaagtccg taccctcatc attcaagatt
    26401 tcgaaaaagt cttcgcggat tacgatttga ttttgggtcc aactgctcca agtgttgcct
    26461 atgacttgga ctcactcaac catgacccag ttgccatgta cttggctgac ctcttgacca
    26521 tacctgtaaa cttagcaggt ttccctggaa tttcgattcc tgctggattc tctcaaggtc
    26581 tacctgtcgg actccaattg attggtccca agtactctga ggaaaccatt taccaagctg
    26641 ctgctgcttt tgaagcaaca acagactacc acaaacaaca acccgtgatt tttggaggtg
    26701 acaactaatg aactttgaaa cagtcatcgg acttgaagtc cacgtagagc tcaacaccaa
    26761 ttcaaaaatc ttctcaccta cttctgccca ctttggaaat gaccaaaatg ccaacactaa
    26821 cgtgattgac tggtctttcc caggagttct accagttctc aataaagggg ttgttgatgc
    26881 cggaatcaag gctgctcttg ccctcaacat ggacatccac aaaaaaatgc actttgaccg
    26941 caagaactac ttctaccctg ataatcccaa agcctaccaa atttctcagt ttgatgagcc
    27001 aatcggttat aacggctgga ttgaagtcga gctagaagac ggtacgacca agaaaattgg
    27061 tatcgaacgc gcccacttag aggaagacgc tggtaaaaac acccacggta cagacggcta
    27121 ctcttacgtt gaccttaacc gccaaggggt acctttgatt gagattgtat ctgaagccga
    27181 tatgcgttcg ccagaggagg cctatgctta tctgacagcc ctcaaggaag ttatccagta
    27241 cgctggcatt tctgacgtta agatggaaga aggttctatg cgtgtggatg ccaacatctc
    27301 ccttcgccct tatggtcaag aaaaattcgg taccaagact gaattgaaga acctcaactc
    27361 cttctcaaac gttcgtaaag gtcttgaata cgaagtccaa cgccaggctg aaattcttcg
    27421 ctcaggtggt caaatccgcc aagaaacacg ccgttacgat gaagctaaca aaacaaccat
    27481 cctcatgcgt gtcaaggaag gggctgctga ctaccgctac ttcccagaac cagacctacc
    27541 cctctttgaa atttctgacg agtggattga ggaaatgcag actgagttgc cagagtttcc
    27601 aaaagaacgt cgtgcgcgtt atgtatctga ccttggttta tcagactacg atgctagtca
    27661 gttgactgct aataaagtca cttctgactt ctttgaaaaa gctgttgccc taggtggtga
    27721 tgccaaacaa gtctctaact ggctccaagg ggaagtcgct cagttcttga atgctgaagg
    27781 taaaacactg gaacaaatcg aattgacacc agaaaacttg gttgaaatga ttaccatcat
    27841 cgaagacggt actatttcat ctaagattgc caagaaagtc tttgtccatc tagctaaaaa
    27901 tggcggtggc gcgcgtgaat acgtggaaaa agcaggtatg gttcaaattt cagatccagc
    27961 tatcttgatc ccaatcatcc accaagtctt tgccgataac gaagctgctg ttgccgactt
    28021 caagtcaggc aaacgtaacg ccgacaaggc ctttacagga ttccttatga aggcaaccaa
    28081 aggccaagcc aacccacaag ttgcccttaa actacttgca caggaattgg cgaagttgaa
    28141 agaaaactag acagaacaaa accagcccta aggttggttt tttcttgaca aatagaaaga
    28201 aaacgtttac aatattgcgt gtaaagtttc caaacaagaa aactaaaaat cgcaacaata
    28261 aggagttatt tatatggcta caatgaaggc agctcgctgg catgcagcga aagatgttcg
    28321 tattcaagaa atagaagttc ctgaagtact tccgcaccaa gtaaaagttg cagttaagtt
    28381 cacaggaatt tgtggtactg acctccatga atttttagat ggacctatct ttatcccaac
    28441 agatgagcac gtctattctg gtcaaaaagc accggtaaca ctgggacatg aattttctgg
    28501 tgaaatcata gaagtcggaa gcgatgttac ctgtgttaaa gttggtgatc gtgttgctgt
    28561 ggaaccaatt ctagctaaaa ataacttagt tggtgattat aatttggatc caaaccttaa
    28621 ttttgtcggt ttggctgcag acggtggatt tgccaaatat tgtgttttag acggtgactt
    28681 agtacatgtc attccagata gcttgagcta tgagcaagct gctcttactg aacccgctgc
    28741 tgttgcagtc tatgctgttc gtcaatcttc tttgaaggct ggcgatacag ctgttgtctt
    28801 tggtttaggc ccgattggtc tattaattgt agaagctctt cgtgcagctg gtgcttctaa
    28861 gatttatgct gttgaactat cacctgaacg tcaagcaaaa gctgaagagt taggagctat
    28921 cgttgtccgt ccggaagaag gtgaaacagc tgttgaagct attcatcgtt taacaaacgg
    28981 tggtgtagat gtttcttatg aagtaacggg tgtgccagta gttttaggac aagccttagc
    29041 tgccgtacat aaagcaggtg agtgcatggt ggtctctatc tgggaacgag aagcaaatat
    29101 taatccgaat gaatttgcaa tccaagaaaa gacactaaaa ggaatcattg cttatcgtca
    29161 tatcttccct aaagtattag aattgatgga acaaggctac ttctccgctg aaaaattagt
    29221 tactaagaaa attaaattgg aaaatatcgt cgaagaaggc tttatcgaat taactcaaga
    29281 taagtcacaa attaaaattt tggtagaacc agaataaaaa atcaatcttt ttaagacaat
    29341 actaacaggt cggagtaaac ttacttcggc cttttgaaat ataatactct tcgaaaatct
    29401 cttcagacca cgtcagcgtc acattaccgt actcaaatac agcttgcggc tagcttccta
    29461 gtttgctctt tgatttccat tgagtatgag aagaaatata gtggatactc tttaacatta
    29521 accactccac ctgactagcc aatattcttt ttcgctccta ccaactccca ataactattt
    29581 tggctttatt tccagagtat tttatggtaa aatgaagagt aataatattt attaaagagg
    29641 taaaaacatg attgaagcaa gtaaattaaa agctggtatg acctttgaaa cagctgacgg
    29701 caaattgatt cgcgttttgg aagctagtca ccacaaacca ggtaaaggaa acacgatcat
    29761 gcgtatgaaa ttacgtgatg tccgtactgg ttctacattt gacacaagct accgtccaga
    29821 ggaaaaattt gaacaagcta ttatcgagac tgtcccagct caatacttgt acaaaatgga
    29881 tgacacagca tacttcatga atacagaaac ttacgaccaa tacgaaatcc ctgtagtcaa
    29941 tgttgaaaac gaattgcttt acatccttga aaactctgat gtgaaaatcc aattctacgg
    30001 aactgaagtg atcggtgtca ccgttcctac tactgttgag ttgacagttg ctgaaactca
    30061 accatctatc aaaggtgcta ctgttacagg ttctggtaaa ccagcaacga tggaaactgg
    30121 acttgtcgta aacgttccag acttcatcga agcaggacaa aaactcgtta tcaacactgc
    30181 agaaggaact tacgtttctc gtgcctaatc tctagaaaga ggtcattcta tgggaattga
    30241 agaacaactt ggcgaaatcg ttatcgcccc acgtgtactt gaaaaaatca ttgctatcgc
    30301 tactgcaaag gtagagggtg ttcactcttt ttcaaacaga tcagtgtctg ataccctttc
    30361 aaaactttca ctcggccgtg gcatttatct taaaaacgtg gacgaagaac tcacagcaga
    30421 tatctatctc taccttgagt acggagtaaa agttcctaag gtagcggttg ctatccagaa
    30481 agctgtcaaa gatgccgtcc gtaatatggc tgatgtagaa ctcgctgcta tcaatattca
    30541 cgttgcaggt atcgtcccag ataaaacacc aaaaccagaa ttgaaagatc tatttgacga
    30601 ggacttcctc aatgactagt ccactattag aatctagacg ccaactccgt aaatgcgctt
    30661 ttcaagctct catgagcctt gagttcggta cggatgtcga aactgcttgt cgtttcgcct
    30721 atactcatga tcgtgaagat acggatgtac aacttccagc ctttttgata gacctcgttt
    30781 ctggtgttca agctaaaaag gaagaactag ataagcaaat cactcagcat ttaaaagcag
    30841 gttggaccat tgaacgctta acgctcgtgg agagaaacct ccttcgcttg ggagtctttg
    30901 aaatcacttc atttgacact cctcagttgg ttgctgttaa tgaagctatc gagcttgcaa
    30961 aggacttctc cgatcaaaaa tctgcccgtt ttatcaatgg actgctcagc cagtttgtag
    31021 cagaagaaca ataaaaagaa aaagtgtttg atagatatca aacacttttt ctttgcctcc
    31081 cactatctaa aaaaatgata atagatataa ttgtaaacaa aaatccagat aggttttgca
    31141 tgattgagaa agttaaaaaa actatggcag agaatcgtta atctcagatt gtcggtagaa
    31201 cgataaacaa aggcaaaaaa gaaaccaatc agactataat ataataaact aattggatct
    31261 ctgtgagata gtatcaaatg actaagccca aagataaggc ctgataagat gacatctaaa
    31321 taatacttgg actagggaaa gaaggtattc ataaaatacc ctctatcaag agtctcctca
    31381 aaaacaggac cgatgattac aggcaggaca aaagataaga tagtcgataa aaaggttggt
    31441 tgtccatttg aaagaaccac ggtaaaatac tcatcatgaa tattcctatg attaatcaaa
    31501 tgagcatagc gtgcccaaaa attaccgaga atctgataaa ccacataagt tgcaaataag
    31561 gagaagacaa atgaccagtt ccagctcttt ttctcaaaga taaagagcat ctttttcttt
    31621 tttaacctcc aaattaatag aaaaggaaac ttcccactaa tcccactgtt aaaataagag
    31681 aatagacatc agctcctaac cctaaaatga tcgtcacata caatccaatt gtttgtggta
    31741 agtaaatagg tagatagtaa aataataagc aaaaatattc caaattgtct tagtttcttt
    31801 gtgtttctca tcgtactttt ttgaaagatt accctgttcg gaagccgtac ttccaagcat
    31861 ctatataaga attaagtgcc ccttgcctca tataaggagc aaattctcta taatataacc
    31921 atctactata tccatcttcc caaacagcaa gaccacctga agtttgctcc aagtcctcag
    31981 ttgaaataac tgtaaatgtt tctgtacctg tcattgcaag taccttctta aaatagattg
    32041 ttgtaggctc acatttatag tatatttctt tttttgtcta ttttatagtc tatctcctca
    32101 actgtcaatt tttcaacctg aattgcattt ttccataaaa aatgagacct ttctagtctc
    32161 atttagtcat tcttagtatt ttctaaatct ttgatagcgt tcttccagca actcttctag
    32221 cgatttttgt gaaagtctag ctagctccgt ttggagttct tttttaacat tcttaatcaa
    32281 ttctttacta gaaagtcccg tttcagagat caccttatcc accacgtcca tttctaacaa
    32341 ctcgtgtgaa gtgattttca tcagttctgc cgcttccatg gcacgactgc cgtccttcca
    32401 tagaatggaa gcaaagcctt ctggactgag aatagcatag atagaatttt ccagcatcca
    32461 gacacggtct gcgactgcta gagccagagc cccacctgaa ccaccttcac cgataatgat
    32521 agcgataata ggaactctca gatcactcat ttccatgaga ttgcgagcaa tggcttctcc
    32581 ctgtccacgt tcttccgctc ccacaccagg ataggcacct gctgtattga taaaggtcac
    32641 aactggacgg ccaaactttt cagcctgttt catcaaacgc aaggccttgc gatagccttc
    32701 tggatgcggt tgtccaaaat tccgcttgag gttatcttgt aaactcttgc ctttttggat
    32761 accaaccact gttacagctt gatcaccaag ccagccaata ccaccaacaa ctgcaccatc
    32821 atcacgaaaa gaacggtcac cgtgtaattg gataaagtca tcaaaaatgc ctgtcgcaaa
    32881 gtccaaagtt gtcaagtgac tctgctcacg cgcttctctg actatttttg caatattcat
    32941 ctaggactcc ctccatgcaa tctgactagg ctagcaatcg tatctggcaa atctcttctt
    33001 ttaacgatag catccacaaa gccatgttcc aataggaatt ctgccttttg gaaatcctca
    33061 ggcaagcttt cacgaaccgt attttcaatg acacgacgcc cagcaaaacc gaccaagctc
    33121 tgtggttcag ccagaataat atcgccttcc atagcgaaag aagctgtcac accaccagtc
    33181 gttggatctg tcaaaatggt caggtaaaag agaccagcat ttgaatggcg tttaactgct
    33241 gcagaaatct tggccatctg catgagactc atgattcctt cctgcatacg ggctccacca
    33301 gaggctgtga agagaacaac tggcaactgt tcaaccgtcg catactcaaa taagcgagtg
    33361 attttttcac ctacaaccgt acccatagaa gccatgataa agttggaatc cataatccca
    33421 agagccacag tctgaccttt aataagagca gttcctgtca caacggcttc atccagacca
    33481 gttttttcac gcatagttgc tagtttcttt tgataaccag ggaaatgcaa gggatccttg
    33541 ctttcaatcc ctgtaaacaa ttccttgaag gttcccatat caatcgtcaa aaccaagcgt
    33601 tcttgggcag aaatacgaaa ggtatagcta cagtgtggac agatacgctc acttcccaga
    33661 tccttctgat agatggtatg cttacaaccc ggacactgag aaaataattc atctggaact
    33721 tctggcttgg cttgaggttt ttccctaatc gaacgattgg gattgattcg aatatactta
    33781 tcttttttac taaatagagc cattgattcc ccttttcggt ttaaactctt gaagtcattt
    33841 tattcttttt cttgatactt gggtaaaaag gtttccatca agaaggaagt atcataatcc
    33901 ccagcaatga cattgcgatc tgaaatgagg tcaagctgga aatctgcatt tgtctgcact
    33961 ccttcaatct ctaattcata aagggcacgt tgcattttca tcaaagcatc aaaacgattt
    34021 tcgccgtgaa cgatgatttt agcaatcata ctatcataat aaggcggaat ggtatagcct
    34081 ggataaactg ctgaatccac gcgcaagcca actccaccac ttggcagata gagattagta
    34141 atcttacctg gacttggagc aaagttaaag gctgggtttt ctgcattgat acgacactcg
    34201 atggcatgac cgcgtaggac aatatcttct tgcttaacag acaaaggctg acctgctgca
    34261 atgcgaattt gttccttaac gatatccaca cctgaaacaa actctgttac tggatgttct
    34321 acttgtacac gagtattcat ctccatgaaa tagaaattgc cacttgcttc atcaagaaga
    34381 aactcaatgg tccctgcatt ctcatagcca acagactctg cagctcgaac agccgcagca
    34441 ccgatttcat ggcgcaacgt ttttccgatt gcaatcgaag gactttcttc caaaaccttt
    34501 tggttattcc gttgaagaga acaatcccgt tcacccaagt gaatcacatg tccatgttca
    34561 tctcctagga tttgaacctc gatgtgacga gctggataga taacccgttc aatgtacatg
    34621 gcaccattgc cataattggc cttggcttca ctagaggcag tttcaaaggc gaaaacgagg
    34681 tcttctgttt tttcaacctt acgaattcct ttaccacctc cacctgctga agccttgagc
    34741 ataacaggat agccaatttt ttctgcaaca atcaaagctt cctcagagtt atgcacttct
    34801 ccatctgaac ctggaatgac aggcacacct gctttgatca tctgagcacg cgcattgatc
    34861 ttgtctccca tcatatccat tacatgacca gatggaccga taaacttgat tcccacctct
    34921 tcacacatgg tcgcaaattt ggaattttcg ctgagaaatc caaaacctgg gtgaatggct
    34981 tctgcctcag tcaagactgc agctgataga accgcattaa tattgagata agactctgtt
    35041 gccttgccag gaccgataca aactgcttca tctgccaaaa gcgtatgaag ggcttcctta
    35101 tcagcagttg aataaaccgc caccgttgcg atccccaatt cacgtgccgc acggataata
    35161 cgaaccgcaa tttcaccacg attggcaatt aaaatttttc gaaacatgga gaacctcctt
    35221 agttcccaat tgcaaaagta agggtaccac tggctgcaag cttgccatcc acttcagcct
    35281 ttgcttcaac cacagctatg gtgccacgac gttttacaaa agtcgctgtc ataaccaatt
    35341 ggtcgcctgg tacaacttgc ttcttgaact taaccttatc cataccagcg taaaagacca
    35401 gttttccttt attttcaggt tttgataact ccaacacacc agcagtttgc gccaaggctt
    35461 ccataatcag aacacctggc ataactgggt attgaggaaa gtggccgtta aagaaaggct
    35521 cgttgatggt cacatttttg atagcaacaa tggtatcctc gctcacctcc aagacacggt
    35581 ccactagaag cataggataa cggtggggaa gggcttcttt gattccttga atatcgatca
    35641 tttgatacgt accaatcctt taccaaactc aaccatttct tcgttagaga cgagaatttc
    35701 cgttaccaca ccatccttag gagctgggat ttcattcatg actttcatgg cttcgataat
    35761 taccaatgtt tgaccttttt tgacactatc accaactgta acgaaggcag gtttatctgg
    35821 tccagcagcc aagtaaacca ctccaacaag tggactctct acaagatttc cctcagtagc
    35881 cacacttgct tcagctggag ctggaacttc ttctgctaca gtctctgctg gagcagatgt
    35941 aggagctact ggactcggtg ttgctagaac gggtgctgga gcgacttgag ttgcaacttc
    36001 aggcacaggt ctcgcttcat tcttgctaaa ctgcaactca tccgtcccat ttttataaga
    36061 aaattctctc aaacttgact ggtcaaattg agtcatcaag tctttaatat cgtttaaatt
    36121 catacttatc tattctccca acgtttgaaa gcaagaactg cattgtggcc tccaaaacca
    36181 aaagtatttg aaatagcgta tggaatttct ttctccaagc cttgtccata aacgacatta
    36241 gcttcgatat aatctgatac ttcacttgtc ccagctgtca ttggtacaaa gttatgacgc
    36301 atagcttcga tggtgacgat agcttctact gcacccgcag cccccagcaa atgtcctgta
    36361 aaagacttgg ttgatgatac aggtacttcc ttaccaagaa cagctacgat agcaccactt
    36421 tctccttttt cattggcagg agttgacgtt ccgtgagcat tgacataggc tacttgctct
    36481 ggagaaatct cagcttcttc caaggctagt ttgatggcct tgatagctcc ctgaccttct
    36541 ggatgtggag aagtcatgtg gtaggcatca caagtatttc cgtaaccaac cacttcagcc
    36601 aggatagtag ctccacgttt ttcagcgtgt tcaagacttt ctagaaccaa catccctgaa
    36661 ccttcaccca taacaaaccc attgcgatcc ttatcaaatg ggatcgaagc acgagttgga
    36721 tcctctgtag tagagagagc tgttaaggct tggaaaccag cgatggcaaa aggtgtgata
    36781 gaagcttctg ttcctcccac caacatcaca tcttggaaac caaacttaat ggagcggaag
    36841 gcatccccaa tcgcatcatt tgatgaagag caggcagtat tgatagattt acaaacaccg
    36901 tttgcaccaa aacgcatggc tacattccca gaagccatat ttggtaaagc ttttggaaga
    36961 gtcattggtt tgacacgttt gggtcctttt tcatgaagac gaagtacctg atcttcaatt
    37021 tccttgattc caccaatacc agatgcaacg ataacaccaa aacgatccct attaagagcc
    37081 tctacatcaa gattggcatg atttacagcc tcttgggctg catacaaggc atataaagaa
    37141 tagttatcaa aacggttggt atcttttttt acaaagtatt tatcgaacgg aaaatcttgg
    37201 atttctgccg cattatgcac atcaaagtca ctatgatcaa attttgtaat gccaccaatg
    37261 ccgattttcc cagttgctaa actattccaa aattcttctg gtgtatttcc gattggagat
    37321 gttactccat aacctgttac cactactcga tttagtttca ttcttttctc ctctagcttt
    37381 cgctacatac ttaagccgcc atcaatggca atcacttgac cagttagata atcttggcct
    37441 gctaaaaata ctgtcaaatc tgcaacctgc tctgcctgcc caaattcttt catcggaatc
    37501 tgagctagtg tagcttcctt aatcttatct gataagatag ctgtcatatc agactcaatc
    37561 attcctggag caatcacatt gactcgtata ttccgactag cgacctcgcg tgccacagac
    37621 ttggtaaagc caatcaagcc agccttagaa gcagcatagt tagcttgacc aatattcccc
    37681 atcaaaccaa caacactaga catattaatg atagcacctt ctctggcttt catcatcggt
    37741 ttcaagactg attgtgtcat attaaaggca ccagtcagat tgaccttgag cactttttca
    37801 aaatctgctt ctgtcatctt gagcataaga gtatcttggg taatccctgc attgttgacc
    37861 aaaacatcta ctgaacccag ttctgcaata gcttgatcaa tcatacgctt agcgtctgca
    37921 aaatctgata catctcctga aatgggaacc accttgatac catagtttga aaactcagcg
    37981 agcaattctt ctgagattac cccacgactg tttaagacaa tgttggctcc tgcttgagca
    38041 aacttgtggg cgatggcaag accaattcca cgactcgaac ctgtaataaa gatattttta
    38101 tgttctagtt tcattttttt cctttcaaaa cttctactta ttttagtcta tttttctaaa
    38161 agtgctacta aactcgcttg atcttccaca tgagctaagt gagcagtttg atcaattttt
    38221 ttaacaaaac ctgacaagac tttccctggt ccaatctcga taaagttgct tatgcctgct
    38281 tcttgcatga ccccaatact ttcatagaaa cgaacgggtt ccttgacctg acgcgtcaag
    38341 agctgagcaa tgtcctcttt ttgcatcaca gcagcttctg tattgccgac taggggacaa
    38401 gtaaaatctg aaaaacttac ctgagctaga gtttcagcta gtttctggct agcaggttca
    38461 aggagagcgg tgtgaaaggg acctgacacc ttaagaggaa tcaagcgttt ggcacctgct
    38521 tcttgcaaaa gttcaaccgc tcgatcaact gcaaccactt ctccagcaat gacgatttgt
    38581 gcaggtgtgt tatagttggc tggagtaacc actccaagtt cagaagcttt ttgacaggct
    38641 tcttcaatga cctctactgg cgtattgaga actgctacca tcttgccaga gtcagcagga
    38701 gccgcttctt ccatataggc tccacgctta gctaccaagg caaccgcatc ttcaaaatcc
    38761 aaggcgccgc ttgacaccaa ggcagagtat tctccaagag acaaaccagc aaccatatca
    38821 ggctgatagc ccttttcttg caataaacgg tagatagcaa ccgaagtcgc tagaatggct
    38881 ggttgcgtat agcgggtctg attgagtttg tcttcttccg tatcgatgag ataacgcaaa
    38941 tcataaccga gcacctgact cgctcgatca atcgtttctt taacaatcgg atactgatca
    39001 tagaaatccc gtcccatccc tagatactgg gcaccttgac cagcaaataa aaaggctgtt
    39061 ttagtcattt cttacaactc ctgtccagcg agaggcttct tcttgaattt tcttagcggc
    39121 tccgtaatac aaatctttta ggatttcttc agctgtttct tctttagaaa caagccctgc
    39181 gatttgacct gccatgacag agccaccatc cacatcaccg tgaacaactg ctttggctag
    39241 ggcacctgct cccatttgtt caaagatttc taaatcagga tcttcctgct taaaggcatc
    39301 tttttcagcc agttcaaaat ctctagtcaa ctgattttta atagcacgaa cagcatgacc
    39361 aaagtgctga gctgaaatcg tagtatcaat atcccttgct tttaaaattt tctccttgta
    39421 gtttggatgg gcattcgact cttttgcaac tacaaaccgt gtccccacct gtacagcctc
    39481 tgcacctagc ataaagccag ccgcagcacc ttcaccatcc gcaattcctc ctgcagcaat
    39541 aacaggaata gatacagctg tggctacctg tcgcaccaag gtcatggttg ttaatttacc
    39601 gatatgcccc ccagcttcca ttccttctgc aataacagcg tctgcaccga ttttttccat
    39661 gcgtttagct aaagcgacac taggaacaac aggaataacg attatcccag cttcatggaa
    39721 acgttccata tacttgcttg gatttcctgc tcctgttgtg acaactttaa caccttcttc
    39781 aataacgaga tccacgatat cttccacaaa gggagataag agcatgatgt tgaccccaaa
    39841 gggtttatca gtcaatgatt tgattttatc aatattggcc ttgacaactt ctttcggggc
    39901 atttccccca ccgataattc ctaatcctcc agccttggaa acagcccctg ccaaatcacc
    39961 atcagcaacc caggccatcc ctccttggaa aataggatag tcaatcttca ataattctgt
    40021 aatacgcgtt ttcatagtgc ctccaacctt ccttgcttac gtaatagttc gatttcacca
    40081 taatttgaca gtcaaactat tacctaaaca agagggagtg ggtttctccc tactccttct
    40141 actaatattc tgcttatttt gcttgctctt caacgtaagc aaccaagtca ccaactgttt
    40201 tcaagtcatt ttctgcttcg atttggatat caaaagcatc ttcgatttct gagattactt
    40261 ggaacaagtc caatgaatct gcgtccaaat catcaaaagt tgattcaagt gttacttctg
    40321 atgcgtcttt tccaagttct tcaacgataa tttcttgtac tttttcaaat actgccatga
    40381 taggactcct ttaaaataaa tagttttttt ataacaatgt gttcaccaca tgattaccta
    40441 aattgtaaga atgagcgtgc cccaggtcaa gcctccaccg aagcctgata gaagaacagt
    40501 ctggctacca tctaaaggga tgagaccttg ttctacacac tctgaaagta aaatcgggat
    40561 actggctgca ctggtattgc catattccat catattggct ggaagtttgg ctcggtcaac
    40621 accaattttt ctagccatct tatccaaaat acggtcattg gcttgatgaa gtagcagata
    40681 atccaagtct gtcacctcta taggagattc atcaatagtc tgcttgatag acttggctac
    40741 atctcgaatg gcaaaatcaa agactgtgcg tccatccatc ttcaaaaatg aatctgcact
    40801 ttcttgatct gaaaatggag aatgcaaacc tgaatgccca taagttaaac actcgctgcg
    40861 acttccatcg ctattgagac tctcagctaa gaaatgctct tgctcgctag cttctaacaa
    40921 gacaccacca gcaccatctc caaacaacac agctgttgat cgatccgacc aatcgactgc
    40981 cttagagagg gtttcactac caatcaccaa gcctttttga aagcgaccag aagcgataaa
    41041 cttttcagca gttgaaagag caaatacaaa tccactgcaa gctgcggtta agtcaaaagc
    41101 aaaggcttta ttagcaccaa tattagcttg aacacgagca gctgtagagg gcatcatcga
    41161 atctggagta atggtagcta ggatgataaa atccagttcc tctcctatta ttccagcttt
    41221 tgccatcagt ttcttagcaa cctctgtagc caaatcactg gtagattctg ttcttgaaat
    41281 atgcctttgt cgtattcccg ttcgacttga aatccactca tcattggtat ccataatctg
    41341 agccaagtcg tgatttgtaa ccacttgctc tggaacataa tgagcaacct ggcttatttt
    41401 tgaaaaagcc attatttcaa atcctccaaa aattggtaaa gattagtcaa acctttaccc
    41461 atgacagcaa tttcttcctc gctcatgcca tcaataattt tttctaccat ggccttgtgg
    41521 aagcgtttat gcagtctatg aatcaagcga cccttctttg tcaaatgcag atgcaccaca
    41581 cgacgatcct gttctgaccg aactcgctca atgtagccct tacgctctaa attgttcaaa
    41641 cttgtcgtaa cagttccaag agttaccatc aactcttttg acacttgact tggagtcacg
    41701 tctggagcct taccgataac atcaatcgta tgcatttctt tgatggagat atccttaaaa
    41761 cgactacctc tcaagttcac ttcctcaatc acaaggacat tgttaaatat agatgttaaa
    41821 tattcattga ttcgttggta gtccattttt catatccctc cttcttcaaa aaacttttac
    41881 aatcaaaaca tttgacaaaa agaattatat caaaattcaa agaatgatgc aagtattttt
    41941 tattttcctg taaatttagg tcttcttctc tccgaatgag ctc
//
LOCUS       DAVKDZ010000009        38681 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000009 DAVKDZ010000000
VERSION     DAVKDZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38681
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.009"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            101..1378
                     /locus_tag="VWZ30_000481"
     CDS             101..1378
                     /locus_tag="VWZ30_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEU9334282.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            1454..2938
                     /locus_tag="VWZ30_000482"
     CDS             1454..2938
                     /locus_tag="VWZ30_000482"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU9334283.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELNYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTAELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCESYS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            2999..4279
                     /locus_tag="VWZ30_000483"
     CDS             2999..4279
                     /locus_tag="VWZ30_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9334284.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTSMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMILKG"
     gene            4283..4939
                     /locus_tag="VWZ30_000484"
     CDS             4283..4939
                     /locus_tag="VWZ30_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9334285.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSMAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLNKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGKHRIQALADISRIFEAERQKDCVSFVTET
                     "
     gene            5069..6211
                     /locus_tag="VWZ30_000485"
     CDS             5069..6211
                     /locus_tag="VWZ30_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9334286.1"
                     /translation="MNKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSVVAEEAPQETTPAEKQETQASPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPESSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            6499..6822
                     /locus_tag="VWZ30_000486"
     CDS             6499..6822
                     /locus_tag="VWZ30_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9334287.1"
                     /translation="MTRYQILLFLLEHSPCNQMAVQERLKINQAALTRHFKILETEGL
                     VERHRNPENQREVLVEAAKYAKEQLVVNPPLQHIKVKEEMESILTESERTELNRLLNK
                     LVLRS"
     gene            6825..7469
                     /gene="pcp"
                     /locus_tag="VWZ30_000487"
     CDS             6825..7469
                     /gene="pcp"
                     /locus_tag="VWZ30_000487"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9334288.1"
                     /translation="MKVLVTGFEPFGGQKGNPTLEVIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVAINQDDARISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLQASVSNTAGTFVCSHLMYQALYLVEKKFPYVK
                     AGFMHIPYMMEQVVNRQTTPAMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(7497..8354)
                     /locus_tag="VWZ30_000488"
     CDS             complement(7497..8354)
                     /locus_tag="VWZ30_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU9334289.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQRFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            8484..9626
                     /gene="tgt"
                     /locus_tag="VWZ30_000489"
     CDS             8484..9626
                     /gene="tgt"
                     /locus_tag="VWZ30_000489"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU9334290.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFRKYFVEKYGYNKSGRNF"
     gene            9727..11544
                     /locus_tag="VWZ30_000490"
     CDS             9727..11544
                     /locus_tag="VWZ30_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU9334291.1"
                     /translation="MRIKWFSLIRITGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALT
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPIVQTLHPLSYTHTILVASTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            11697..12848
                     /gene="nagA"
                     /locus_tag="VWZ30_000491"
     CDS             11697..12848
                     /gene="nagA"
                     /locus_tag="VWZ30_000491"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU9334292.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            13011..14069
                     /locus_tag="VWZ30_000492"
     CDS             13011..14069
                     /locus_tag="VWZ30_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU9334293.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSATILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            14220..14585
                     /locus_tag="VWZ30_000493"
     CDS             14220..14585
                     /locus_tag="VWZ30_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEU9334294.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            14661..15602
                     /locus_tag="VWZ30_000494"
     CDS             14661..15602
                     /locus_tag="VWZ30_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU9334295.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKIASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            15550..16566
                     /locus_tag="VWZ30_000495"
     CDS             15550..16566
                     /locus_tag="VWZ30_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU9334296.1"
                     /translation="MDILQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDRQCVTQMRVGLSQGKSFSEMMESLGCSSAIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
                     LEMIVFVSVGVLLALTFYKRSSKMRVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            16568..16894
                     /gene="comGC"
                     /locus_tag="VWZ30_000496"
     CDS             16568..16894
                     /gene="comGC"
                     /locus_tag="VWZ30_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEU9334297.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLRKLQADGRITEEQAKAYKEYHDKNGGA
                     NRKVND"
     gene            16863..17291
                     /locus_tag="VWZ30_000497"
     CDS             16863..17291
                     /locus_tag="VWZ30_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU9334298.1"
                     /translation="MEEQIVKSMIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVE
                     EQIFFMEFEELYRETQKRSVASQQKTSLNLDGQMISNGSQKLTVPKGIQAPSGQSITF
                     DRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            17296..17556
                     /locus_tag="VWZ30_000498"
     CDS             17296..17556
                     /locus_tag="VWZ30_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334299.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            17519..17980
                     /locus_tag="VWZ30_000499"
     CDS             17519..17980
                     /locus_tag="VWZ30_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9334300.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVESNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            17958..18371
                     /locus_tag="VWZ30_000500"
     CDS             17958..18371
                     /locus_tag="VWZ30_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9334301.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            18508..19095
                     /locus_tag="VWZ30_000501"
                     /pseudo
     CDS             18508..19095
                     /locus_tag="VWZ30_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            19156..20109
                     /locus_tag="VWZ30_000502"
     CDS             19156..20109
                     /locus_tag="VWZ30_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9334302.1"
                     /translation="MDFEKIEQAYIYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFTEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGF
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKVWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            20160..21350
                     /locus_tag="VWZ30_000503"
     CDS             20160..21350
                     /locus_tag="VWZ30_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU9334303.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPKKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEVAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            21499..21870
                     /gene="rnpA"
                     /locus_tag="VWZ30_000504"
     CDS             21499..21870
                     /gene="rnpA"
                     /locus_tag="VWZ30_000504"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU9334304.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGGLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            21845..22669
                     /locus_tag="VWZ30_000505"
     CDS             21845..22669
                     /locus_tag="VWZ30_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9334305.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQTQKDLENRKRKAKKKAQKTK"
     gene            22688..23674
                     /locus_tag="VWZ30_000506"
     CDS             22688..23674
                     /locus_tag="VWZ30_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEU9334306.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVNAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVNDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     ANRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            23725..24348
                     /locus_tag="VWZ30_000507"
     CDS             23725..24348
                     /locus_tag="VWZ30_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEU9334307.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQTKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            24479..25936
                     /locus_tag="VWZ30_000508"
     CDS             24479..25936
                     /locus_tag="VWZ30_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9334308.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKSTVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            25959..26240
                     /locus_tag="VWZ30_000509"
     CDS             25959..26240
                     /locus_tag="VWZ30_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9334309.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            26316..26801
                     /locus_tag="VWZ30_000510"
     CDS             26316..26801
                     /locus_tag="VWZ30_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9334310.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            26818..27483
                     /locus_tag="VWZ30_000511"
     CDS             26818..27483
                     /locus_tag="VWZ30_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEU9334311.1"
                     /translation="MTKRIPNLQVALDYSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     IKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAVDPAGAARAFKDEIKR
                     IWG"
     gene            27487..28350
                     /locus_tag="VWZ30_000512"
     CDS             27487..28350
                     /locus_tag="VWZ30_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEU9334312.1"
                     /translation="MVRPIGIYEKAIPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKAPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            28352..29056
                     /locus_tag="VWZ30_000513"
     CDS             28352..29056
                     /locus_tag="VWZ30_000513"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU9334313.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            29235..30887
                     /locus_tag="VWZ30_000514"
     CDS             29235..30887
                     /locus_tag="VWZ30_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9334314.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVV"
     gene            31020..32111
                     /gene="ulaG"
                     /locus_tag="VWZ30_000515"
     CDS             31020..32111
                     /gene="ulaG"
                     /locus_tag="VWZ30_000515"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEU9334315.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKVTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            32225..34201
                     /gene="tkt"
                     /locus_tag="VWZ30_000516"
     CDS             32225..34201
                     /gene="tkt"
                     /locus_tag="VWZ30_000516"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9334316.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLR
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFVQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSIPSTDVFDKQDAAYKEEILPNAVRRRVAIEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            34318..34617
                     /gene="yajC"
                     /locus_tag="VWZ30_000517"
     CDS             34318..34617
                     /gene="yajC"
                     /locus_tag="VWZ30_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9334317.1"
                     /translation="MNPNYTFLIMLALMVGLIFFTQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            34664..35107
                     /locus_tag="VWZ30_000518"
     CDS             34664..35107
                     /locus_tag="VWZ30_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEU9334318.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQQYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNVSDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYQRVQEGCKVWLERLEKESEDGKS"
     gene            35094..35504
                     /locus_tag="VWZ30_000519"
     CDS             35094..35504
                     /locus_tag="VWZ30_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334319.1"
                     /translation="MENLKSFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     ILKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            35804..38455
                     /gene="adhE"
                     /locus_tag="VWZ30_000520"
                     /gene_synonym="adhC"
     CDS             35804..38455
                     /gene="adhE"
                     /locus_tag="VWZ30_000520"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEU9334320.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWISQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGHNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVKKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        11839 a         6891 c         8691 g        11260 t
ORIGIN      
        1 agtgttagga acgttgttat tcttcgttcc ttttttatat tatttttggt ataattatag
       61 ttattcaaat tttatttaga ttaagaaagt gtaggggagt atgtcttgtt ctatcgattt
      121 attaaaacat cggtatttga aaaatattaa agaaaatcct gaattgtttg tcggaattga
      181 gttggagtat cctgttgcaa gtttagaagg ggatgctaca gatgttgaag ttataaagga
      241 tctatttcat tatttagttt ctactttgga tctcaccgta gcaaaggtag atgattttgg
      301 caatctgatc cagttagtag atccgataag tcaggatgct attttatttg aagtttccta
      361 tacaacgatt gagtttgcat ttggtaaggc tgaaacgatt caagaggtcg aaaatcgttt
      421 caataattat atgaatgtaa ttcagagaaa gttagctgaa tcaaatcatg ctattgttgg
      481 ctgtggtatc catcccaact gggataaaaa tgagaattgt ccagtggctt atccacgcta
      541 tcagatgttg atggattatt tgaatttgag tagaaatatt attaaatcag atttacatca
      601 tttccctgaa tacggtactt ttatctgtgg gagccaggtt cagctggata tttcaaaaac
      661 caactacttg cgggtgatta atgcttttac tcaaattgaa gcggctaagg cttatttatt
      721 tgcaaactct gagttttcgg gtgcggattg ggatacgaaa atttcaaggg atattttctg
      781 ggaagaatct atgcatggta tctatccaga gaatgttggg gtcaatgcta gactccttaa
      841 tgatgaagct gatttttttg actatctaaa tcattctgcg atttttactg cggaacgtga
      901 tgggcagacc tattattttt atcctattca ggctggggac tatttggcta cgcctgaaat
      961 ccaagcattt gctctgaatg gggatgaggt tattatttac ccccaagaga aggattttga
     1021 aactcatcgt agttaccagt accaagactt aacgactcga ggaacagttg agtttcgtag
     1081 tgtgtgtaca cagccacttg ataggacttt tgcttctgca gcttttcact tgggattatt
     1141 ggttaattta gacaagttag aagcttactt agaaacagca cctttcttta aagtatttgg
     1201 ttatgattac aagtctttaa ggagacaatt ttctaagaaa aatcttacag atgaggaaga
     1261 aactacgatt attgaatttt ccaaagactt actcctacta gctgaggagg gactagtggt
     1321 gagaaataag gaagaaatga cctatttaca gcctttgaga gaagaattga gcctataatt
     1381 tctcttataa agggagaatt ttctgaaaaa tcatgatata atggacgaga ctatagataa
     1441 aggatagaga gtaatgacat tagtttatca atcaacgcgt gatgccaaca atacagtaac
     1501 tgccagccaa gcaattttgc aaggtttggc gacggacggc ggtttgttta caccggttac
     1561 ttatccaaag gtagatttga actttgacaa attgaaagat gcttcttacc aggaagttgc
     1621 taagctagtt ttgtcagcat ttttagatga ctttacagtt gaggagttga actactgtat
     1681 caacaatgcc tacgatagca aatttgatac tccagctatt gcaccattag tgaaattaga
     1741 tgggcaatac aatttggaac ttttccatgg ttcaacgatt gcctttaagg atatggcctt
     1801 gtctattttg ccatacttta tgacgactgc tgctaagaaa catggtttgg agaacaagat
     1861 tgttatcttg acagcgacat ctggtgacac ggggaaagct gctatggcgg ggtttgcgga
     1921 tgtgcctggt actgagatta tcgtctttta tccaaaggat ggtgtcagca agattcaaga
     1981 gttacaaatg accactcaga ctggcgacaa tactcatgtt attgctattg atggtaactt
     2041 tgacgatgcg caaacaaatg tgaagcacat gtttaacgac gtggctcttc gtgaaaaatt
     2101 gactaccaac aagttgcaat tttcatctgc taactctatg aatattggtc gtctggtgcc
     2161 acaaattgtt tattatgttt atgcttacgc tcaattggtt aagactggtg aaattgtagc
     2221 tggtgaaaag gttaacttca cagtaccaac aggaaacttt ggaaatatct tggctgcctt
     2281 ttatgccaaa caaattggtt tgccagttgg taaattgatc tgtgcttcaa atgacaacaa
     2341 tgttttgaca gacttcttta aaacacgtgt ctatgacaaa aaacgtgagt ttaaggtaac
     2401 aaccagccca tctatggata tcttggtatc ttcaaacttg gagcgcttga ttttccatct
     2461 tttgggaaat aatgctgaaa agacagctga actcatgaat gctttgaaca cgcaaggaca
     2521 atataagttg acagactttg atgcagagat tttggacctc tttgcagctg aatatgcgac
     2581 tgaggaagaa acggcagcag agatcaagcg tgtttgtgag tcatattctt atatcgagga
     2641 ccctcataca gctgttgctt cagcagttta taaaaaatac caatcggcca ctggagatgt
     2701 aactaagaca gtgattgctt caacagctag tccatacaag ttcccagtag ttgcagtaga
     2761 agctgtaact ggaaaagcag gtttaacaga ctttgaagcc ttggctcaat tacatgaaat
     2821 ctcaggcgtt gcagtgccac cagcagttga tgggcttgaa atagctccaa ttcgtcacaa
     2881 gacaacagtg gcagctgctg acatgcaagc agcggttgag gcttatttag gactttaaga
     2941 cagagggagc aaactcggtt gggaaaccaa ctgagtttct tttcatcagg aggagagatt
     3001 gtttaagaaa aataaagaca ttcttaatat tgcattgcca gctatgggtg aaaacttttt
     3061 gcagatgcta atgggaatgg tggacagtta tttggttgct catttaggat tgatagctat
     3121 ttcaggggtt tcagtagctg gtaatattat caccatttat caagcgattt tcatcgctct
     3181 gggagctgct atttccagtg ttatttcaaa aagcataggg cagaaagacc agtcgaagtt
     3241 ggcctatcat gtgactgagg cgttgaagat taccttacta ttaagtttcc ttttaggatt
     3301 tttgtccatc ttcgctggga aagagatgat aggacttttg gggacggaga gggatgtagc
     3361 tgagagtggt ggactgtatc tatctttggt aggcggatcg attgttctct taggtttaat
     3421 gactagtttg ggagccttga ttcgtgcaac gcataatcca cgtctgcctc tctatgttag
     3481 ttttttatcc aatgccttga atattctttt ttcaagtcta gctatttttg ttctggatat
     3541 ggggatagct ggtgttgctt gggggacaat tctgtctcgt ttggttggtc ttgtgatttt
     3601 gtggtcacaa ttaaaactgc cttatgggaa gccaactttt ggtttagata aggaactgtt
     3661 gaccttggct ttaccagcag ctggagagcg acttatgatg agggctggag atgtagtgat
     3721 cattgccttg gtcgtttctt ttgggacgga ggcagttgct gggaatgcaa tcggagaagt
     3781 cttgacccag tttaactata tgcctgcctt tggcgtcgct acggcaacgg tcatgctgtt
     3841 ggcccgagca gttggagagg atgattggaa aagagtcgct agtttgagta aacaaacctt
     3901 ttggctttct ctgttcctca tgttgcccct gtcctttagt atatatgtct tgggtgtacc
     3961 attaactcat ctctatacga ctgattctct agcggtggag gctagtgttc tagtgacact
     4021 gttttcacta cttggaacct ctatgacgac aggaacagtc atctatacgg cagtctggca
     4081 gggattagga aatgcacgcc tcccttttta tgcgacaagt ataggaatgt ggtgtatccg
     4141 cattggaaca ggatatctga tggggattgt gcttggttgg ggcttacctg gtatttgggc
     4201 agggtctctc ttggataatg gttttcgctg gttatttcta cgctatcgtt accagcgcta
     4261 tatgatcttg aaaggatagg aaatgcaaaa aacagctttt atttgggatt tagacgggac
     4321 tttattggac tcttacgaag cgattttatc agggattgag gagacttttg ctcagttttc
     4381 tattccttat gataaggaga aggtgagaga gtttatcttc aagtattcgg tgcaagattt
     4441 gcttgtgcgg gtggcagaag atagaaatct ggatgttgag gtgctaaatc aggtgcgtgc
     4501 ccagagtatg gctgagaaga atgctcaggt agttttgatg ccaggtgcgc gtgaggtgct
     4561 agcttgggca gacgaatcag gaattcagca gtttatatat actcataagg ggaacaacgc
     4621 ttttaccatt ctcaaggact tgggggtgga atcctatttt acagagattt taaccagtca
     4681 gagtggcttt gtgcggaagc caagtccaga agcggctacc tatctgctaa ataagtatca
     4741 gttgaattct gataatactt attatatagg ggatcggact ctggatgtgg aatttgccca
     4801 gaatagtggg attcaaagta tcaacttttt agagtcgact tatgaaggga aacacaggat
     4861 tcaagcgtta gcagatattt cccgtatttt tgaggctgag cgacaaaaag attgtgtcag
     4921 ttttgtgaca gagacctaac aaactatttc aagtaaccta gtttgttaca aggaatagac
     4981 aattttgtta aataggcccg agagggcttt ttttctgcat tttttgtgtt atcatagaca
     5041 ggtactcatt tgaaaggaat ttgaaagaat gaataaaaga atgttattag cgtcaacagt
     5101 tgccttgtca tttgccccag tattggcaac tcaagcagaa gaagttcttt ggactgcccg
     5161 tagtgtagag caaatccaaa acgatttgac taaaacggac aacaaaacaa gttatacagt
     5221 acagtatggt gatactttga gcaccattgc agaagctttg ggtgtagatg tcacagtgct
     5281 tgcgaatctg aacaaaatca ctaatatgga cttgattttc ccagaaactg ttttgacaac
     5341 gactgtcaat gaagcagaag aagtaacaga agttgaaatc caaacacctc aagcagactc
     5401 tagtgaagaa gtgacaactg cgacagcaga tttgaccact aatcaagtga ccgttgatga
     5461 tcaaactgtt caggttgcag acctttctca accgattgca gaagctccaa aagaagtagc
     5521 atcaagttca gaagttacaa agacagtgat tgcttctgaa gaagtggcac catctacggg
     5581 cacttctgtc ccagaggagc aaacggccga aacaagcagt gtagttgcag aagaagctcc
     5641 tcaggaaacg actccagctg agaagcagga aacacaagca agccctcaag ctgcatcagc
     5701 agtagaagca actacaacaa gttcagaagc aaaagaagta gcatcatcaa atggagctac
     5761 agcagcagtt tctacttatc aaccagaaga gacgaaaata atttcaacaa cttacgaggc
     5821 tccagctgcg cccgattatg ctggacttgc agtagcaaaa tctgaaaatg caggccttca
     5881 accacaaaca gctgccttta aagaagaaat tgctaacttg tttggcatta catcctttag
     5941 tggttatcgt ccaggagaca gtggagatca tggaaaaggt ttggctatcg actttatggt
     6001 accagaaagt tcagaattag gagataagat tgcggaatat gctattcaaa acatggccag
     6061 ccgtggcatt agttacatca tctggaaaca acgtttctat gctccattcg atagcaaata
     6121 tgggccagct aacacttgga acccaatgcc agaccgtggt agtgtgacag aaaatcacta
     6181 tgatcacgtt cacgtttcaa tgaatggata aacccgactt gataacatca ttttgacgaa
     6241 tgagatctag ctttcgtgat ggaaagcgat tctcgttcgt ttttttcttt gtcaatttga
     6301 atggaagaag taagaatctt gtaatgactc aagcaacatt cttgcaatct attttacttt
     6361 acattatact tgattagtca actattgatg aatcaagaaa aagagaaaag agatgttaga
     6421 gattcaagat ttactttatc aactccgctt gtctgagcaa gcgagcacgc aattgtttga
     6481 aaaaggcttg ggattagttt gacacggtat cagattttac tgtttttgct ggagcattct
     6541 ccttgtaacc aaatggcggt tcaggagcgt ttgaaaatta atcaggctgc tttgacacgg
     6601 catttcaaga ttttggaaac ggaaggtttg gtggagcgtc atcgtaatcc tgaaaatcag
     6661 cgagaagtgt tggtagaggc tgcgaagtat gccaaggagc agttagtggt gaatccccct
     6721 ctgcaacata tcaaggttaa ggaagagatg gaaagtatct taacagagtc tgagagaaca
     6781 gagctcaacc gtttattaaa taaattggtt ttgaggagtt agaaatgaaa gtattagtga
     6841 caggttttga gccctttgga gggcaaaagg gcaatccaac tttggaggtc attaaaggtt
     6901 taccagctga aatccatggt gctgaggtcc gttggctaga ggtgccgaca gtttttcaca
     6961 aatctgctca agtattggaa gaagagatga atcgttatca acctgacttt gtcctttgta
     7021 ttgggcaagc tggtggaaga actagtttga cacctgaacg agtggccatt aatcaagacg
     7081 atgcacgcat ttctgataac gaagataatc aaccgattga ccgtcccatt cgcccagatg
     7141 gtgcttcggc ctactttagt agtttgccga ttaaagcgat ggttcaagct ataaaaaaag
     7201 agggcttaca ggcctctgtt tccaatacgg cagggacttt tgtctgcagc catttgatgt
     7261 atcaggctct ctatttggta gaaaagaaat ttccatatgt taaggcaggt tttatgcata
     7321 ttccttatat gatggaacag gtggtgaaca gacagactac tccagctatg agtttagtgg
     7381 atattcggcg agggatagaa gcagcaatcg gcgctatgat agaacatgga gatcaggaac
     7441 tcaagttggt aggcggagaa actcattgat agaaaaaagc ttgaggggaa aaaccttcaa
     7501 gcttttggac gttttcgagc caatactgct cggtaaaaca taattttagt gcattggata
     7561 taaggtagga gtgaaaaact agcaatgcca aaggtaatcc aattgaggaa gtaccaagga
     7621 agaagctgta aatctaggac aaagcgctgg aacttgtagc ccttcataaa ggaacggcta
     7681 gtttttagga ttcgtcttgg tgggacctgt cctaggtcta aactataaca gagaagaaat
     7741 tccacctgtg aataggcata atactgtgga atatagagga tatttcctac aatgatcaag
     7801 atgagacttg caagaaagta gagtccaaag accatgagga aacgctcggt ttcaactgat
     7861 gagagatcta gatttggaaa ctcaggatgt agggtgacga attttttggc taaaaagcta
     7921 ctataaaaga ggaggtaaat cccaagtaaa ttagggatac tccataaaaa gagatagaaa
     7981 cgtttgagaa gtagggtcaa aaaggtttga gaaaagcgct cctcatcaaa gagagctagg
     8041 ctgtttttta cagatggctc cgttttagaa tctttcatga gtgtcagtgt tgcatagacg
     8101 gaactggtca aaagaatagt cccgataaag gagactagta gaggaaagag gtaggcttga
     8161 agtatttggc caagtatgct gaaaaatggc tgttctaaaa cagtcccgtg gatccgagat
     8221 aagggattaa gaaaactaga taagatgacc agcatactgg gaaggatata gaggagaaag
     8281 agacgggggg tgtcagcctg aaaatgtttt gactcctgac gaattgtttt taaatcaatt
     8341 tttggatagt tcattctctt attataccat agttcttata catagttcgt gacagttcct
     8401 actttttttg ataaaatcat acagtgtgtc cttgggcaca ctgtatgaac tgggagtgtc
     8461 tttcccagct tcggaggtaa aaaatgtcag attcaccaat caaatatcgt ttgattaaga
     8521 aagaaaaaca cacaggagct cgtctaggag aaatcatcac tccccacggt acctttccga
     8581 cacctatgtt tatgccagtt gggacacaag ccactgtcaa aactcagtca cctgaagaat
     8641 tgaaggagat gggttcggga attatcctat caaacaccta tcatctctgg cttcgccctg
     8701 gagatgaact cattgcacgc gctggtggtc tccacaagtt catgaattgg gaccagccta
     8761 tcttgacaga tagtggtggt tttcaggttt attctttagc agatagccgt aatatcacag
     8821 aagaaggagt aacctttaaa aatcatctaa atggttctaa gatgttccta tccccagaaa
     8881 aagccatctc tattcagaat aatctgggtt cagacatcat gatgtccttt gatgaatgtc
     8941 ctcagtttta tcaaccttat gactacgtta agaaatcgat cgagcgtacc agccgttggg
     9001 ctgagcgtgg tttgaaggct caccgtcgtc cacatgacca aggtttgttt ggaattgtgc
     9061 aaggtgcagg atttgaagac cttcgccgcc aatcagctca tgatcttgtc agcatggatt
     9121 tctcaggcta ctctatcggt ggtttggcag tgggagaaac ccatgaagag atgaatgcgg
     9181 tcttggactt tacaactcaa ctgctgcctg aaaataaacc tcgttatctg atgggtgtgg
     9241 gagcgccaga tagcttgatc gatggggtca ttcgtggggt ggatatgttt gactgtgtct
     9301 taccgactcg aattgctcgt aacgggactt gtatgaccag tcaaggacgt ttggttgtga
     9361 aaaatgccca gtttgctgag gactttacgc cactggatcc tgagtgtgat tgctacacat
     9421 gtaataacta tacacgcgct taccttcgtc acctgctcaa ggctgatgaa acctttggta
     9481 tccgcttgac tagctaccac aatctttact tcttgcttaa cctgatgaag caagtgcgac
     9541 aagccatcat ggatgacaat ctcttggaat tccgtaaata ttttgtggaa aaatatggct
     9601 ataataagtc aggacgtaat ttctaaaatg gaattaatat aagctaaaaa tcctaagttt
     9661 tctcttagga tttttcttct ttttttgata gaataaagtg tacaatgaaa ggaagaataa
     9721 actcgtatgc gcattaaatg gttttccttg attaggatta caggtttact tttggtactc
     9781 ttgtatcatt tctttcagac catctttcct ggaggatttt tcggggtaga tgtctttttc
     9841 acattttcag gtttcctgat tacagctcta ctcattgaag aattttctaa gaacaatgag
     9901 attgatttga taggattttt tagaagacgc ttttatcgga ttgtgccacc tgtggttttg
     9961 atggtcttgg tgaccatgcc ttttactttc ttggttcgcc aagactatgt tgctggaatt
    10021 ggtggccaga ttgcgggcgt cttaggcttt atgaccaact tctatgaact cctaacaggt
    10081 gggagttatg aatctcagtt cattcctcat ttgtttgttc ataattggag tttggcagtt
    10141 gaggttcact actatattct ttggggattg gcagtttggt tcttatccaa acaagctaaa
    10201 tcaaatggtc agttgaaggg gatggtcttt ctcttatctg ctgctgcctt cttgatcagt
    10261 ttcttctcca tgtttattgg tagttttcta gtgacctctt attcctctgt ttatttctcc
    10321 agtttaactc atgtctatcc attctttttg ggaagtatgt tagcaactat tgtaggcgtt
    10381 cgtcagacga cttccctcgt caagcagttg gataaaatct gggatttacg aaagactttg
    10441 gtagtttttg gaggaggctt tggtttccta gttcttttga ctttctttgt caaattcact
    10501 tatctttttg cctatcttat cggcttcttg cttgccagtc ttgcagctct taccatgatt
    10561 ctggcggcgc gtgtcttaca tgaaaagaca catcatatac aggagccgaa gattatcagc
    10621 tttttagcgg atactagcta tgcggtttat cttttccatt ggcctttcta tatcattttc
    10681 tcacagttga catcaaatct tcttgctgta ttactgactc tgatttgttc ttatggcttt
    10741 gccagtctgt cattctatgt attggaacct tggattgcag gcaagaacac acctattgtc
    10801 caaacccttc atcccctgtc ctatacccat acaatccttg tagcaagtac aggaattttg
    10861 accatcattg tctgcacggt gaccttgttg gcaccacaag tgggagcgtt tgagacagac
    10921 ttgactgtca atggcttgaa gcaagctgca acaaatattg gccagaccaa ggtgatggca
    10981 gaacgggcag atgcaaacag tttgggaatt gctgatggca cgatgttaat tggtgactca
    11041 gtggctttaa gggcaaatac agcactacag acagctcttc ctggagcaca gattaacgcg
    11101 caggtcagcg taacaaccaa gaccgcaaat gaaatcatgc taaataatag ccagaataaa
    11161 tttttaccta agacggtggt cattgcgact ggggtaaata atcctgagaa ttacaaggat
    11221 gactgggaca gtatcgtgaa aaatcttcct aagggacacc atatgatttt ggtgactcct
    11281 tatgagggag ataagacaaa agagacctat gccatcgttg agaaggctgc tgcctatatg
    11341 agagaattgg cagagaagac accttacatt acgatagcag attggaatca agttgcgaaa
    11401 gagcatccag aaatttgggc tggaacagac caggttcatt tcgggagtga gagtagcact
    11461 atcgaagcag gagcaaaatt gtatgcagat acgattgcca cagctttgca gacggctcaa
    11521 gacaagccgg ttaaatcaaa ataacttgta ttaaaaaaga gaagagttgg agaaatccag
    11581 ctcttttaaa atatctccaa aaaataggtc tataccattt acaaataaaa aagaaaggtt
    11641 tataatgtaa ttgacataat aaattgtaga atcaatcttt taaggaggtt aacattatgc
    11701 ctaactatat taaagcggat cagtttttct acccacacgg agttcgtcga ggtggttact
    11761 tggaacttgt ggacggcaag tttggaaaac atgtagagca gattcctgaa ggggctgagg
    11821 tgattgacta tacaggttat agcattgccc caggtcttgt ggatactcat attcatggat
    11881 atgcaggtgt agatgtgatg gacaacaaca ttgaaggtac attgcatact atgagtgaag
    11941 gacttcttag taccggtgtt accagtttct tacccacaac tttaacagcc acttatgagc
    12001 aattgcttgc agtcactgaa aatcttggaa accattataa agaagcaaca ggtgctaaga
    12061 ttcgtgggat ttattatgaa ggtccatatt tcacagaaac ttttaagggg gcacaaaatc
    12121 caacttatat gagagacccg ggtgttgagg agtttcattc ttggcaaaaa gcggcaaatg
    12181 gcttgcttaa taaaattgcc cttgcaccag aacgtgatgg ggtggaagac tttgttcgta
    12241 cagttacggg cgaaggtgtg acggttgctc ttggacattc aaacgcgact tttgatgaag
    12301 ccaaaaaagc agtcgatgct ggagcgagtg tttgggtgca tgcctacaat ggaatgcgtg
    12361 ggttgactca ccgtgaattg ggtatggttg gagccatgta ccaattgcca catacctatg
    12421 cagagttgat ctgtgatggt caccacgtag atccaaaggc ttgcgaaatt cttatcaaac
    12481 aaaaaggaac agaaaacatc gctcttatca cagactgtat gacagctggg ggattggaag
    12541 acggagatta tatgttggga gaattcccag ttgtcgttgc aaatggaact gcacgcctca
    12601 aatcgacagg taacttggca ggttctatcc tcaaactcaa agatggtttg aagaatgtgg
    12661 tcgaatgggg aattgcgaat ccgcatgaag cagtcatgat ggccagcttc aacccagcta
    12721 aatccgttca catcgatgac gtctgtggcc aaatccgtga aggctacgac gctgacttca
    12781 tcgtattaga taaagatttg gaattggtag caacctacct agatggcgta aaacgttatc
    12841 aagcataaga gagaaagcag aagttagaga ctaacttctg cttttttatg cagggtactt
    12901 tactggtaaa taaaaagtta aaaaaatcac aaaaaaagct tgaagaaaca aagaaaaagg
    12961 cttacaattg aactataaat agtacaaata aaaaaacgga ggagtgcttt atgaaagcct
    13021 atacttatgt taaaccagga cttgcttctt ttgttgatgt agacaaacca gttattcgca
    13081 agccaacaga tgctattgtg cgtattgtaa aaaccactat ttgtggaaca gacctccata
    13141 ttatcaaagg ggatgttcct acttgccaaa gtgctaccat tcttggccac gaagggattg
    13201 ggattgttga agaagttggg gaaggagttt ccaacttcaa aaaaggtgac aaggtcttga
    13261 tttcttgcgt ctgtgcctgt ggtaaatgct actactgtaa aaaaggaatt tatgcccact
    13321 gtgaagacga agggggctgg attttcggtc acttgattga tggtatgcag gctgaatatc
    13381 tacgtgtccc tcatgcagat aatactcttt accatactcc agaagacttg tcagatgaag
    13441 ctttggttat gctgtcagac attctgccta ctggatatga aattggtgtc ttaaaaggga
    13501 aagtagaacc tggttgcagc gtagccatta ttggttcagg tccagttgga ttggctgctc
    13561 ttttaacagc ccaattctat tcaccagcta aattgattat ggtagaccta gacgataacc
    13621 gcttggaaac tgccctatca ttcggtgcga ctcataaggt taattcttca gaccctgaaa
    13681 aagccattaa agaaatttat gatttgacag atggtcgtgg tgtggatgtc gctatcgaag
    13741 ctgttggtat tcctgcaaca tttgatttct gtcaaaagat tatcggtgta gacggaacgg
    13801 ttgccaactg tggtgtgcat ggtaaaccag ttgaattcga tttagataaa ctttggattc
    13861 gcaacatcaa tgtaacaact ggtttggtat ctacaaatac gactccacaa ttgttgaaag
    13921 cacttgaaag tcataagatt gaaccagaaa aattggtaac tcactatttc aaactcagtg
    13981 aaattgaaaa agcctatgaa gtcttcagta aggcagcaga ccaccatgcc attaaggtca
    14041 ttatcgaaaa cgatatctca gaagcctaag tagtaaaaat atttttgtac ataagcaaat
    14101 agaaattcag tcatccatca gatggctgga ttttttatca aaaaattaag aaatgagcat
    14161 atttctttcc ttgtctggcg gaattggtta taatatacgg tacaaaggaa tgaatgaata
    14221 tgtatcgtgt tatagaaatg tacggagatt ttgaaccgtg gtggttctta gaaggttggg
    14281 aagaagatat tgtagcaagt agaaaatttg accagtatta tgatgctctc aaatactaca
    14341 aaacttgctg gtttagattg gaacaagaat cccctcttta taaaagtaga agcgacttga
    14401 tgaccatttt ttgggacccg gaagaccaac gctggtgtga tgaatgtgat gagtatttac
    14461 aacaatacca ttctttggct cttttgcagg atgagcaggt tatcccagac gaaaaactac
    14521 gctcaggcta tgaaaaacaa accagtcagg aaaggaatcg ttcttgccgt atgaaattaa
    14581 aatagagaaa agtaactttt ttggagttgc tttttttatt tttctaactc tttgcgaata
    14641 gtataggtga ggaggtaagt atggttcaag aaattgcaca agaaatcatt cgttcagctc
    14701 ggaaaaaagg gacgcaggat atctattttg tccctaagtt agacgcctat gagcttcata
    14761 tgagggtagg agacgagcgc tgtaaaattg gtagctatga ttttgaaaag tttgcagccg
    14821 ttatcagtca ctttaagttt gtggcgggta tgaatgtggg agaaaaaaga cgtagtcaac
    14881 tgggttcctg tgattatgcc tatgaccaga aaatagcgtc tctacgttta tctactgtag
    14941 gcgattatcg ggggcatgag agtttggtta tccgtttgtt gcacgatgag gagcaggacc
    15001 tgcatttttg gtttcaggat attgaagaat taggcaagca gtacaggcaa cgggggctct
    15061 atctttttgc tggtccggtt gggagtggta agacgacctt gatgcatgaa ttgtccaagt
    15121 cactctttaa aggacagcaa gttatgtcca tcgaagatcc tgtcgaaatc aagcaggacg
    15181 acatgcttca gttgcagttg aacgaagcaa tcggcctaac ctatgaaaat ctaatcaaac
    15241 tttccttgcg tcatcgacca gatctcttga ttatcggaga aattcgtgat agcgagacgg
    15301 cgcgtgcagt ggtcagagct agtttgacag gtgcgacagt cttttcaacc attcatgcca
    15361 agagtatccg aggtgtttat gagcgtctgc tggagttggg tgtgagtgaa gaagaattgg
    15421 cagttgtttt gcaaggagtc tgctaccaga gattaatcgg gggaggagga atcgttgact
    15481 ttgcaagcag agattatcaa gaacaccaag cagccaagtg gaatgagcaa attgaccagc
    15541 ttcttaaaga tggacatatt acaagtcttc aggctgagac ggaaaaaatt agctacagct
    15601 aagcaaaaaa atatcatcac cctatttaac aatctctttt ctagcggttt tcatctggtg
    15661 gagactatct cctttttaga taggagtgct ttgttggaca ggcagtgtgt gacccagatg
    15721 cgtgtgggct tgtctcaggg gaaatcattc tcagaaatga tggaaagttt gggatgttca
    15781 agtgccattg tcacccagtt atccctagct gaagttcatg gaaatctcca cctgagtttg
    15841 ggaaagatag aagaatatct ggacaatctg gccaaggtca agaaaaaatt aattgaagta
    15901 gcgacctatc ctttgatttt gctgggtttt cttctcttaa ttatgctggg actacggaat
    15961 tacctgctcc cacaactgga tagtagcaat attgccaccc aaattatcgg taatctgcca
    16021 caaatctttc tagaaatgat agtttttgtt tcagtaggtg tccttttagc actcactttt
    16081 tataaaagaa gttctaagat gcgcgtcttt tctatcttag cgcgccttcc ctttattgga
    16141 atctttgtgc agacctattt gacagcctat tatgcacgtg aatgggggaa tatgatttca
    16201 cagggaatgg agttgacgca gatttttcaa atgatgcagg aacaaggttc ccagctcttt
    16261 aaagaaatcg gtcaagatct ggctcaaacc ctgaaaaatg gccgtgaatt ttctcagacg
    16321 ataggaacct atcctttctt taggaaggaa ttgagtctca tcatagagta tggggaagtt
    16381 aagtccaagc tgggtagtga gttggaaatc tatgctgaaa aaacttggga agcctttttt
    16441 acccgagtca accgcactat gaatttggtg cagccactgg tttttatctt tgtggcactg
    16501 attatcgttt tactttatgc ggcaatgctc atgcccatgt atcaaaatat ggaggtaaat
    16561 ttttaaaatg aaaaaaatga tgacattctt gaaaaaagct aaggttaaag cttttacatt
    16621 ggtggaaatg ttggtggtct tgctgattat cagcgtgctt ttcttgctct ttgtacctaa
    16681 tctgaccaag caaaaagaag cagttaatga caaaggaaaa gcagctgttg ttaaggtggt
    16741 ggaaagccag gcagaacttt atagcttaga aaagaatgaa gatgctagcc taagaaagtt
    16801 acaagcagat ggacgcatca cggaagaaca ggctaaagct tataaagaat accatgataa
    16861 aaatggagga gcaaatcgta aagtcaatga ttaaggcctt taccatgctg gaaagtctct
    16921 tggttttggg tcttgtgagt atccttgcct tgggcttgtc cggctctgtc cagtccactt
    16981 ttgcagcggt agaggaacag attttcttta tggagtttga agaactctat cgggaaaccc
    17041 aaaaacgcag tgtagctagt caacaaaaga ctagtctgaa cttggatggg cagatgatta
    17101 gcaatggcag tcaaaagttg acagttccta aaggaattca ggcaccatca ggccaaagta
    17161 ttacatttga ccgagctggg ggcaattcgt ccctggctaa ggttgaattt cagaccagta
    17221 aaggagcgat tcgctatcaa ttatatctag gaaatggaaa aattaaacgc attaaggaaa
    17281 caaaaaatta gggcagtgat tttactggaa gcagtagtcg ctctagctat ctttgccagc
    17341 attgcgaccc tccttttggg acaaattcaa aaaaataggc aagaggaagc aaaaatcttg
    17401 caaaaggaag aagtcttgag ggtagctaag atggccttgc agacggggca aaatcaggta
    17461 agcatcaacg gagttgagat tcaggtattt tctagtgaaa agggattgga ggtctaccat
    17521 ggttcagaac agttgttggc aatcaaagag ccataaggtc aaggctttta ccttgttaga
    17581 atccctgctt gccctcattg tcatcagtgg gggattactc ctttttcaag ctatgagtca
    17641 gctcctcatt tcagaagttc gctaccagca acaaagcgag caaaaggagt ggctcttgtt
    17701 tgtggaccaa cttgaggcag aattagaccg ttcgcagttc gaaaaagtag aaagcaatcg
    17761 cctatacatg aagcaagatg gcaaggacat cgccatcggt aagtcaaagt cagacgattt
    17821 tcgtaaaacc gatgctagtg gacgggggta tcagcctatg gtttatggcc tcaaatcagc
    17881 tcagattaca gaggacaatc aactggttca ttttcgtttc cagtttcaaa aaggcttaga
    17941 aagggagttc atctatcgtg tggaaaaaga agaaagttaa ggcaggtgtt ctcctctacg
    18001 cagtcaccat agcagccatc tttagtcttt tgttgcaatt ttatttgaac cgacaaatcg
    18061 cccactatca agactatgct ttaaataaag aaaaattggt tgcttttgcc atggctaaac
    18121 gaaccaagga taaagccgag caagaaagtg gggaacaggt ctttaatcta ggtcaggtaa
    18181 gctatcaaaa caagaaaaca agcttggtga cgactgttcg tacgtctaag agccaatatg
    18241 agtttctgtt tccttcagtc aaaatcaaag aagagaaaag agataaaaag gaagaggtag
    18301 cgaccgattc aagcgaaaaa gtggagaaga aaaaatcaga agagaagcct gaaaagaaag
    18361 agaattccta gtcaattcaa ctataatgcg ttgaatccag aatagtccac tgtagtttct
    18421 agaaaattgc tggaaatgga tgttaagctc caattcattt gtttatatct tatttcagtt
    18481 tactatactt tgtgctaaac taaagatatg aaacatgatt ttaaccacaa agcagaaact
    18541 ttcgactccc ctaaaaatat cttcctcgca aacttggtat gtcaagcagt cgagaaacag
    18601 attgatattt tatcagacaa agtaatttta gatttcggtg gtggcacggg tctattagcc
    18661 ttgcccctag ccaagcaggc taagtcagtt actcttgtag acatttctga gaaaatgttg
    18721 gagtaagctc gtttgaaagc ggagcagcaa gcaatcaaga atatccagtt tttggagcaa
    18781 gatttaccga aaaatccctt ggagaaagag tttgattgcc ttgctgttag tcgggttctt
    18841 catcatatgc ctgatttgga tgcggatctc tcactgtttc atcaacattt gaaggaagat
    18901 gggaaactca tcattgctga ttttaccaag acagaagcta atcatcatgg atttgattta
    18961 gctgaactgg aaaacaagct aattgagcat ggtttttcat ctgtgcatag tcagattctc
    19021 tatagcgctg aagacctgtt tcaagggaat cactcagaat tctttttaac agtatcccaa
    19081 aaatcactcg cctagtcagg gagtgatttt tctataagga tggaaaaaag aagggaaatt
    19141 tgataagata ggaatatgga ttttgaaaaa attgaacaag cttatatcta tttactagag
    19201 aatgtccaag tcatccaaag tgatttggcg accaactttt atgacgcctt ggtggagcaa
    19261 aatagcatct atctggatgg tgaaactgag ctaaaccagg tcaaagacaa caatcaggcc
    19321 cttaagcgtt tagcactacg caaagaagaa tggctcaaga cctaccagtt tctcttgatg
    19381 aaggctgggc aaacagaacc cttgcaggcc aatcaccagt ttacgccaga tgctattgcc
    19441 ttacttttgg tatttattgt ggaagagttg tttacagagg aggaaattac tatcctcgaa
    19501 atgggttctg ggatgggaat tctaggcgct actttcttga cctcgcttga taaaaaggtg
    19561 gattacttgg gaatggaagt ggatgatttg ctgattgatc tggcagctag catggcagat
    19621 gtaattggtt tgcaggctgg ctttgtccaa ggagatgccg ttcgcccaca aatgctcaaa
    19681 gaaagcgatg tggtcatcag tgacttgcct gttggctatt atcctgatga tgccgttgcg
    19741 tcgcgccatc aagttgcttc tagccaagaa catacttacg ctcatcactt gctcatggaa
    19801 caagggttta agtaccttaa gtcagatgga tatgctattt ttctagctcc gagtgatttg
    19861 ttgaccagtc ctcaaagtga tttgttgaaa gtctggttga aagaggaggc aagtctggtt
    19921 gctatgatta gtttacctga aaatctcttt gctaatgcta aacaatctaa gactattttt
    19981 atcctacaga agaaaagtga aatagcagtg gagccttttg tttatccact tgctagcttg
    20041 caagatgcaa gtgttttaat gaaatttaaa gaaaattttc aaaaatggac tcaaggtact
    20101 gaaatataaa atagattttg ttataatagt tgaaaacgct taaaaagggg tatcatgtta
    20161 tgacaaaaac aattgcaatc aatgcaggaa gttcaagttt gaaatggcaa ttatacttaa
    20221 tgccagaaga aaaagtattg gcgaaaggtt tgattgaacg tatcggtttg aaagattcaa
    20281 tttcaactgt aaaatttgac ggccgttctg aacaacaaat tttggatatt gaaaatcata
    20341 cacaagccgt taaaatttta ttggatgact tgattcgttt cgatattatc aaggcttatg
    20401 acgagattac aggtgttgga catcgtgttg ttgctggtgg agaatatttc aaagaatcaa
    20461 cagttgttga gggagatgtt ttagaaaaag ttgaagagtt gagtttgttg gctcctctac
    20521 acaacccggc caatgcagca ggtgttcgtg ccttcaagga attgttgcca gacattacca
    20581 gtgtagttgt ttttgatact tccttccaca caagtatgcc aaagaaagct tatcgctacc
    20641 ctctaccaac aaaatattac acagaaaaca aggttcgtaa atacggtgct catggtacaa
    20701 gtcaccagtt tgtagcagga gaagctgcaa aactcttggg acgtccatta gaagacttga
    20761 agttaattac ctgtcatatt ggtaacggag gctcaattac agctgtgaaa gccggcaaat
    20821 ctgtagacac ttctatgggg ttcactcctc ttggtggtat tatgatggga acgcgtacag
    20881 gggatattga tccagctatc attccttatt taatgcaata tacagaggat tttaacacac
    20941 cagaagatat cagtcgtgtt cttaaccgtg aatcaggtct tttgggagtt tctgctaatt
    21001 ctagcgatat gcgcgatata gaagcagctg tagcagaagg gaatcacgag gctagcttgg
    21061 cttatgaaat gtatgttgac cgtatccaaa aacatatcgg tcagtacctt gcagtgctaa
    21121 atggagcaga tgccattgtt ttcacagcag gtgtcggtga aaatgcagag aatttccgtc
    21181 gtgatgtaat ctcagggatt tcgtggtttg gttgtgatgt tgatgatgaa aagaatgtct
    21241 ttggcgttac aggagacatc tcaacagagg tagctaaaat ccgtgtcttg gttattccaa
    21301 cagatgaaga attagtcatt gcccgtgacg ttgaacgctt gaaaaaataa gtgaaactaa
    21361 aaaaatattc aatacaagga gttgggaaag ttatttttcc agcttctttt tctgatgaaa
    21421 ttgtccaaaa ccttgctatg attggctttt ttgaaaaata tggtataata gtagtaattt
    21481 aatagatgga gttgagtttt gaagaaaaac tttcgtgtaa aaagagagaa agattttaag
    21541 gcgattttca aggaggggac aagttttgct aatcgcaaat ttgtggtcta ccaattagaa
    21601 aaccagaaaa accattttcg agtaggtcta tcagttagca aaaaactggg gaatgccgtc
    21661 actagaaatc aaattaagcg acggattcgg catattatcc agaatgcaaa agggggtctg
    21721 gtagaagatg tcgactttgt tgtcattgct cgaaaaggag tcgaaacctt gggatacgca
    21781 gagatggaga aaaatctact ccatgtatta aaattatcaa agatttaccg ggaaggaaat
    21841 gggagtgaaa aagaaactaa agttgactag tttgctagga ctgtctctgt taatcatgac
    21901 agcctgtgcg actaatgggg taactagcga tattacagcc gaatcggctg atttttggag
    21961 taaattggtt tacttctttg cggaaatcat tcgcttttta tcgtttgata ttagtatcgg
    22021 agtggggatt attctcttta cggtcttgat tcgtacagtc ctcttgccag tctttcaggt
    22081 gcaaatggtg gcttctagga aaatgcagga agctcagcca cgcattaagg cgcttcgaga
    22141 acaatatcca ggtcgagata tggaaagcag aaccaaacta gagcaggaaa tgcgtaaagt
    22201 atttaaagaa atgggtgtca gacagtcaga ctctctttgg ccgattttga ttcagatgcc
    22261 ggttattttg gccctgttcc aagccctatc aagagttgac tttttaaaga caggtcattt
    22321 cttatggatt aaccttggta gtgtggatac aacccttgtt cttccgattt tagcagcagt
    22381 attcaccttt ttaagtactt ggttgtccaa caaagctttg tctgagcgaa atggcgctac
    22441 gactgcgatg atgtatggga ttccagtctt gatttttatc tttgcagttt atgcgccagg
    22501 tggagtcgcc ctatactgga cagtgtctaa tgcttatcaa gtcttgcaaa cctatttctt
    22561 gaataatcca ttcaagatta tcgcagagcg cgaggccgta gtacagacac aaaaagattt
    22621 ggaaaataga aaaagaaaag ccaagaaaaa ggctcagaaa acgaaataaa taaggaggaa
    22681 tctggtaatg gtagtattta caggttcaac tgttgaagaa gcaatccaga aaggattgaa
    22741 agaattagat attccaagaa tgaaggctca tatcaaagtc atttctaggg agaaaaaagg
    22801 ctttcttggt ctatttggta aaaaaccagc ccaagtggat attgaagcga ttagtgaaac
    22861 gactgttgtc aaagcaaatc aacaggtagt aaaaggcgtt ccgaaaaaaa tcaatgattt
    22921 gaacgagcct gtgaagacgg ttagtgaaga aaccgttgac cttggtcatg tggttaatgc
    22981 tattaaaaaa atagaggaag aaggtcaagg tatttctgat gaagtcaagg ctgaaatctt
    23041 aaaacatgaa agacatgcca gcactatctt agaagaaact ggtcacattg agattttaaa
    23101 tgaacttcaa atcgaggaag cgatgaggga agaagcaggc gctgatgacc ttgaaactga
    23161 gcaagatcaa actgaaaatc aagacttgaa agagatgggc ttgaaggtcg agcaaagtta
    23221 tgatattgcc caggtggcta cggatgtgac tgcctatgtt caagcgattg tgaatgacat
    23281 ggatgttgaa gctacacttt caaatgatta taaccgtcgt agcatcaatc tacaaattga
    23341 caccaacgaa ccaggtcgta ttatcggcta ccatggtaaa gtcttgaagg ccttgcaact
    23401 gttggctcaa aattatcttt acaaccgcta ttccaaaacc ttctacgtta caatcaatgt
    23461 caatgattat gtcgaacacc gtgcagaagt cttgcagacc tatgcgcaaa aattggcgaa
    23521 tcgtgttttg gaagaaggtc gcagtcataa aacagatcca atgtcaaata gcgaacgcaa
    23581 gattatccat cgtattattt cacgtatgga tggcgtgact agttactctg aaggtgatga
    23641 gccaaatcgc tatgttgttg tagatacaga ataagtaaaa tcagggttga cctgattttt
    23701 tgctagttag aggaggttaa actgatgttg aataagataa gagactatct agacttttct
    23761 ggtttgcagt accgtagtcc tgataaagcg ggagcagagc gagagaagat gctggcgttc
    23821 cgccacaaag gacaagaggc ccgaaaggct tttacagaac tggccaaagc ctttcaagca
    23881 agccatccag aatggcaact ccaacagact agtcagtgga tgaatcaggc ccagcgtttg
    23941 agaccacatt tttgggttta tctacagaga gacggacaag tgacagaacc tatgatggcc
    24001 ttacgtttgt atgggacatc tactgacttt ggaatttctt tggaagtcag tttcatcgaa
    24061 cgtaagaagg atgagcaaac actgggcaag cagaccaaag ttttagacat tccaaccgtt
    24121 aaagggattt attatctaac ctactctaat ggtcaaagtc aacggtggga ggcgaatgaa
    24181 gaaaagcgtc gtactttacg cgagaaggtg aggagtcaag aagttcgaaa agttttagtg
    24241 aaggtagatg ttcctatgac agaaaattcg tctgaagaag aaatcgtaga aggcttattg
    24301 aagtcttatt ctaaaattct tccctattat ctagctacga gaaaataaga taatttgtaa
    24361 aacatcataa atcatacagt ccaagagtga acagtccgct gtgtaattct tggtcttttt
    24421 gtttgcgctt tcgcattata taataaactt acaaaaacaa ttcaaaagga gaacaattat
    24481 ggaagtcgtt tcaagtgttc taaattggtt ttctagcaat attttgcaga atcccgcatt
    24541 tttcgtaggt ttattggtgt tgataggata tgcacttttg aaaaaacctg cccatgacgt
    24601 tttttcaggg tttgttaaat caacagtagg gtatatgttg cttaacgtgg gtgctggtgg
    24661 tttggttaca acctttcgtc caatcttagc agctcttaac tacaaattcc aaattggtgc
    24721 agcggttatc gacccttact ttggacttgc tgcagcaaac aacaaaattg cagcagagtt
    24781 tccagatttt gttggaactg caactacagc tctattgatt ggttttggaa taaatatctt
    24841 gctcgtagct cttcgaaaga ttacgaaggt aagaaccctc tttattactg gtcacatcat
    24901 ggtacaacaa gctgcaacag tatctcttat ggttctattc ttagtaccac aattgcgcaa
    24961 tgcttacggt acagcagcga ttggtatcat ctgtggactt tactgggcag ttagttcaaa
    25021 tatgactgtt gaggcaactc aacgcttgac tggtggtggc ggatttgcga ttggtcacca
    25081 acagcaattt gcaatctggt ttgtagataa agtagcagga cgctttggta agaaagaaga
    25141 aagtttagac aatcttaaat tacctaagtt cctctcaatc ttccacgata cagttgttgc
    25201 atctgctacc ttgatgctcg tattcttcgg agccattctt ttaatcttgg gtccagacat
    25261 tatgtctaat aaagaagtca tcacttcagg aactctattc aatcctgcta aacaagattt
    25321 ctttatgtac attatccaaa cagcctttac cttctcagtt tacttgttcg ttttgatgca
    25381 aggtgtccga atgttcgtat ctgagttgac aaacgccttc caaggtattt caaacaaatt
    25441 gttgccaggt tcattcccag cggttgacgt tgcagcttct tatggatttg gttctccaaa
    25501 tgctgtcttg tcaggattta cctttggttt gattggtcaa ttgattacaa ttgttttgct
    25561 catcgtcttt aaaaatccga ttcttattat tacaggattt gtaccagtgt tctttgacaa
    25621 tgcagccatt gcagtctacg ctgataaacg cggcggatgg aaagcggctg ttatcctttc
    25681 ctttatatca ggtgtccttc aagttgctct aggagctctt tgtgtggccc ttctcgattt
    25741 ggcatcttat ggtggctacc atggaaatat cgactttgaa ttcccatggc ttggatttgg
    25801 atatatcttc aaataccttg gtattgttgg ttatgtactt gtgtgtctct tcttgcttgt
    25861 tattcctcaa cttcaatttg ccaaagcaaa agataaagag aaatattaca acggtgaagt
    25921 tcaagaagaa gcttagtatc tagaaaagga gaaataaaat ggttaaagta ttagcagcgt
    25981 gcggaaatgg aatgggttca tcaatggtta tcaagatgaa ggttgaaaat gctctccgta
    26041 agcttaatca aacagatttt acagtcaatt catgcagtgt cggtgaagct aaaggtttag
    26101 cagtaggata tgacatcgta atcgcttctc ttcatttgat tcaagaattg gaagggcgaa
    26161 ctaatgggaa gttaattggg cttgataact tgatggatga taaagaaatc accgaaaaac
    26221 tcagtcaagc actacagtaa aaggttggag ggggctggac agaaactgag agttatcgtt
    26281 tctgtccttc tccctcttaa ataaaggagg cagatatgaa tttaaaacaa gctttaattg
    26341 acaatgactc gatccgacta ggtttagagg ctaacaattg gaaagaagca gtcaaggtag
    26401 cagtagatcc cttaattgaa agtggggcaa ttttgccaga gtattacgat gctatcattg
    26461 aatcgactga agagtatggg ccttactata tcttgatgcc aggtatggct atgccccacg
    26521 ctagacctga agctggtgtg caaagtgatg ccttttcatt gattacctta caaaatcctg
    26581 ttgtattttc agatgggaaa gaggtatctg ttttgttggc actagcagca acaagctcaa
    26641 aaattcacac aagtgtagcc attccacaaa ttattgccct gtttgaatta gaagattcta
    26701 ttgcacgttt acaggcttgc cagactaaag aagatgtctt ggctatgatt gaagaatcta
    26761 aggatagccc ttatctcgaa ggattggatt tggaaagtta gaaagaggaa taaagaaatg
    26821 acaaaaagaa tacctaattt acaagttgca ttagactatt cagacttgca aggagcgatt
    26881 aaagcagctg tttctgttgg tcaggaagta gatattatcg aagctggaac tgtttgcttg
    26941 cttcaagttg gaagtgaact ggctgaagtc ttgcgtagcc ttttcccaga taagattatt
    27001 gtggcagaca caaaatgtgc tgatgctggt ggaacagttg ctaaaaataa tgcggttcgt
    27061 ggagcagact ggatgacttg tatctgttgt gcaaccatcc ctactatgga agcagctcta
    27121 aaggctatca agactgaacg aggagaacga ggcgaaatcc agatcgagct ttatggcgat
    27181 tggacttttg aacaagctca gctttggcta gatgcaggta tctcacaagc tatttatcac
    27241 caatctcgtg atgctcttct tgctggtgaa acttggggtg aaaaagacct taataagatt
    27301 aaaaaactca ttgacatggg cttccgtgta tctgtaacag gtggtctaga tgtagatact
    27361 ctcaaactct ttgaaggtgt tgatgtcttt acctttatcg caggtcgtgg aattacagag
    27421 gctgtggatc cagcaggagc agcgcgtgcc ttcaaggatg aaatcaaacg aatttggggg
    27481 taaatcatgg tacgtccaat tggaatttat gaaaaggcaa tcccaacaca ctttacttgg
    27541 ctagaacgtt taaattttgc caaggagtta ggctttgatt ttgtcgagat gtctattgac
    27601 gaacgtgacg agcgtttagc aagacttgac tggagtaagg aagaacgctt ggaagttgtc
    27661 aaagcaatct atgaaactgg tgttcgtatt ccttctatct gtttttcagg ccatcgtcgc
    27721 tacccattgg gatcaaaaga tccagttcta gaggaaaaat ctctagaact tatgaaaaaa
    27781 tgtatcgaat tagctcaaga cttgggagtt cgtacgattc aattagctgg ttacgatgtt
    27841 tactatgagg aaaaggcacc ccagacacgc caacgtttta tcaaaaattt gagaaaagcc
    27901 tgtgactggg ctgaagaagc tcaggtggta cttgctattg aaattatgga tgatcctttc
    27961 atcaatagca tcgaaaaata tttggctata gaaaaagaga ttgactctcc cttcctcttt
    28021 gtatatccag atattggtaa tgtgtctgca tggcataatg atatctatag tgagttttat
    28081 cttggtcatc atgccatcgc agctctccat ctcaaggata cttatgcagt gacagaaagt
    28141 tcaaagggcc agttccgaga tgtacctttc gggcaaggtt gtgtcaaatg ggaagaagct
    28201 ttcgatattt taaaggaaac caattataat ggacctttcc taatcgaaat gtggtctgaa
    28261 aattgtgaaa cagtagaaga aacacgcgca gccattcaag aggcgcaagc ttttctctat
    28321 ccactcatta agaaagcagg tttgatgtaa gatgaatcaa gtaatcaatg ctatgcgtaa
    28381 acgagtctgt gatgccaatc aatcattgcc aaaacatgga cttgtcaaat ttacctgggg
    28441 gaatgtatct gaagtcaatc gcgaactcgg tgtcattgtt atcaaaccat caggcgtgga
    28501 ttatgacgaa ttgacacctg aaaacatggt agtgactgat ctagatggta agatcctaga
    28561 aggggattta agaccatctt ccgacctccc aactcatgtg caattatata aggcttggtc
    28621 agaaattggt agtgtggttc acacccattc gacagaagct gttggttggg ctcaggcagg
    28681 tcgtgatatt cctttctacg gaacaaccca tgcagattat ttctacggtt caatcccttg
    28741 cgcccgtagt ttgaccaagg acgaagtaga agtggcctat gaaaaagata ctggcctggt
    28801 tatcgtagaa gagtttgaac atcgcggact taacccggtt gaagtaccag gaattgttgt
    28861 acgcaatcac ggtccattca cctggggcaa aaatccagag aatgctgttt atcactctgt
    28921 cgtactagag gaagtatcaa agatgaatcg ctttacagaa caaatcaatc caagagttgg
    28981 acctgctccc cagtacatac tagaaaaaca ctaccaacgt aaacatggac caaatgctta
    29041 ttatggtcaa aagtaagaac gatgaaggag gagaaaaaga taaatttagc tcctcttttt
    29101 acatttgatt tttattgaga gtaaagttgg agttgaagta attttaaaag attttttaga
    29161 aatagcgctt gatatatata tggtaaaata aaaagaattg ctgtgatatc aatagatttg
    29221 ggggattttt taatatggta ctggataagg caagttgtga tttgcttcaa tatttgatgg
    29281 atcaagaaac gtccaaaacg attatggcga tttcgaaaga tttgaaagag tcaagaagga
    29341 aaatttatta tcacattgac aaaatcaatg ctgctctggg tgacgaggcg cttcacatca
    29401 ttagtattcc acgaattggt attcacttaa cggaagagca gagagatgct tgttgtaaac
    29461 tattatcgga agtagattcg tacgattata tcatgagtgc gcatgaacgt atgatgataa
    29521 tgttactatg gataggtatt tctaaagaac gtattacgat tgaaaaattg atagagttaa
    29581 cagaggtatc taggaatact gttctcaatg atttgaatag tattcgttat caactaactt
    29641 tggaacaata tcaggtgatc ttgcaagtga gcaagtcaca gggataccac cttcatgccc
    29701 accctcttaa taaaattcag tatcttcaat cgcttctata tcatattttt atggaagaaa
    29761 atgccacttt tgtatctatt ttagaagata agatgaaaga gaggttagat gatgagtgtt
    29821 tgctttctgt tgaaatgaac caatttttta aggaacaggt tcctttagtt gaacaagatt
    29881 tagggaagaa aataaatcat catgaaataa cttttatgtt gcaggttcta ccttatttgc
    29941 tgttaagctg tcataatgtt gaacagtatc aagaaagaca tcaggatata gagaaagaat
    30001 tttctttgat aagaaaaaga atagagtatc aggtgtctaa gaaattagga gaacggttgt
    30061 ttcaaaagtt tgaaatttct ttgtcaggac ttgaagtttc tcttgtagct gttctcctcc
    30121 tctcctatcg taaagatttg gatattcatg cagaaagtga tgattttcgg caattaaaac
    30181 ttgctttaga agaatttatc tggtattttg aatcacaaat ccgaatggag attgagaaca
    30241 aggatgattt gttacgaaat ttgatgatcc actgtaaagc cttgttattt agaaagactt
    30301 acggtatttt ttctaaaaat cctctaacaa aacaaattcg atccaagtat ggagaattat
    30361 ttttagtcac tagaaaatct gcggaaattt tagaaggagc atggtttatt cggctaacag
    30421 acgatgatat tgcctatttg acgattcata ttggaggatt tttaaagtat acaccatcgt
    30481 ctcaaaaaaa tatgaaaaaa gtttatctcg tttgtgatga aggtgttgcg gtttcgagac
    30541 ttttgctgaa acaatgcaaa ctttattttc caaatgagca aattgacact gtatttacaa
    30601 cagaacaatt taagagtgtg gaagatattg cacaagttga tgtagtgatt actactaatg
    30661 atgatttgga tagcagattt ccgattttaa gggttaatcc tatccttgaa gcagaagata
    30721 ttttgaaaat gctagactat cttaaacaca atatatttcg taataagagc aaaagtttca
    30781 gtgaaaatct ttctagtctt atttcgtctt atattgtaga cagcaagttg gctagtaagt
    30841 tccaagaaga ggttcaaaca cttataaatc aagaaatagt agtttaatct tttttggaag
    30901 atatttgaag gacagtccaa tgatgaacac aaacctgtgt ttttcttggt cttttttagt
    30961 gttttgaagg gtggtatact aatctcaaag ataacaatta tatccaaagg aggcaacata
    31021 tgccaaacgt caaagaaatt acaagagagt catggatttt agccactttc ccagagtggg
    31081 gaacatggtt gaacgaagaa atcgaagaag aagtcgtacc tgaaggcaac tttgccatgt
    31141 ggtggctagg caactgtggt acttggatta agacaccagc tggtgctaac gttgtcatgg
    31201 acctttggtc aaaccgtgga aaatcaacca aaaaagtgaa agatatggtt cgtgggcacc
    31261 aaatggcaaa tatggcaggt gttcgtaagc tgcaaccaaa cttgcgtgtt cagccaatgg
    31321 ttatcgatcc atttgctatc aacgaactag actattactt agtttcacac ttccacagtg
    31381 atcatatcga cccatacaca gctgcagcaa ttctcaacaa tcctaagtta gagcatgtta
    31441 agtttatcgg tccttaccac tgtggacgaa tctgggaagg atggggtgtt ccaaaagaac
    31501 gtatcatcgt tgttaaacca ggtgacacta tcgaattaaa agatatgaag attcatgcag
    31561 tagaatcatt tgaccgtact tgcttggtaa ctctcccagt gaacggtgct gatgagacag
    31621 gcggtgaact tgctggctta gctgttacag atgaggaaat ggctcaaaag gctgttaact
    31681 atatctttga aacaccaggt ggaaccatct atcacggtgc agattctcac ttctcaaact
    31741 attttgcaaa acatggtaaa gactttaaaa ttgatgttgc tttgaataac tatggtgaaa
    31801 atccggtagg tatccaagac aaagtgacat ctatcgacct tcttcgtatg gcagaaaatc
    31861 tgcgtaccaa agtcattatc ccagttcact atgatatttg gtctaacttc atggcttcta
    31921 ctaatgagat tctagaactt tggaaaatgc gaaaagatcg cttgcaatac gatttccatc
    31981 catttatctg ggaagttggc ggtaagtaca cttatcctca agatcaacac ttagtagaat
    32041 accatcatcc acgtggtttt gatgattgtt ttgaacaaga ctctaacatt caatttaaag
    32101 ctttgttata atataaaaat attctttgga ggttatccgt atgttgcaac atacggataa
    32161 ttttcatata atagtaaaat agaatgtgtg attcaataat cacctcaaat agaaaggaaa
    32221 ttctatgtca aatctatctg ttaatgcaat tcgtttccta ggtattgacg ctatcaacaa
    32281 agccaactca ggtcacccag gtgtggttat gggagcggct ccgatggctt acagcctctt
    32341 tacaaaacaa cttcgtatca atccagctca accaaactgg attaaccgtg accgttttat
    32401 tctttcagct ggtcatggtt caatgctcct ttatgctctt cttcaccttt ctggttttga
    32461 agatgtcagc atggatgaga ttaagagctt ccgtcaatgg ggttcaaaaa caccaggtca
    32521 cccagaattt ggtcatacgg cagggattga tgctacgaca ggtcctctag ggcaagggat
    32581 ttcaactgct actggttttg tccaagcaga acgtttcttg gcagctaaat ataaccgtga
    32641 aggctacaat atctttgacc actatactta cgttatctgt ggagacggag acttgatgga
    32701 aggtgtctca agcgaggcag cttcatacgc aggcttgcaa aaacttgata agttggttgt
    32761 tctttatgat tcaaatgata tcaacttgga tggtgagaca aaggattcct ttacagaaag
    32821 tgttcgtgac cgttacaatg cctacggttg gcatactgcc ttggttgaaa atggaacaga
    32881 cttggaagca atccatgctg ctatcgaaac agcaaaagct tcaggcaagc catctttgat
    32941 tgaagtgaag acggttattg gatacggttc tccaaacaaa caaggaacta atgctgtaca
    33001 cggcgcccct cttggagcag atgaaactgc atcaactcgt caagccctcg gttgggacta
    33061 cgaaccattt gaaattccag aacaagtata tgctgatttc aaagaacatg ttgcagaccg
    33121 tggcgcatca gcttatcaag cttggactaa attagttgca gattataaag aagctcatcc
    33181 ggaactggct gcagaagtag aagccatcat cgacggacgt gatccagtcg aagtgactcc
    33241 agcagacttc ccagctttag aaaatggttt ttctcaagca actcgtaatt caagtcaaga
    33301 tgccttgaat gttgtggcag ctaaattacc aaccttccta ggtggatcag ctgaccttgc
    33361 tcactcaaac atgacttata tcaagacgga tggacttcaa gacgatgcca atcgcttgaa
    33421 tcgtaacatt cagtttggtg ttcgtgaatt tgcaatggga acaatcttga acgggatggc
    33481 ccttcatggt ggacttcgtg tatacggtgg aactttcttc gtcttctctg actatgtgaa
    33541 ggcagctgtc cgcttgtcag ccttacaagg acttcctgtg acttatgtct ttacccatga
    33601 ttcaatcgca gttggggaag atggtccgac tcacgaacca gttgagcatt tagcaggtct
    33661 tcgtgctatg ccaaatctaa atgttttccg tccagcagat gcgcgtgaaa ctcaagcagc
    33721 ttggtacctt gcagtgacaa gtgagaaaac accaactgcc cttgtcttga cacgtcaaaa
    33781 cttgactgtt gaagatggaa cagacttcga caaggttgct aaaggtgctt atgttgtata
    33841 tgaaaatgca gccgactttg ataccatctt gattgcgaca ggttcagagg ttaatcttgc
    33901 tgtctcagct gctaaagaat tggctagtca aggcgaaaaa atccgcgtag tcagcattcc
    33961 atctacagat gtctttgata aacaagatgc agcttacaag gaagaaattc ttccaaatgc
    34021 agtccgccgt cgtgttgcaa ttgaaatggg tgcaagtcaa aactggtaca aatatgttgg
    34081 tctcgatggt gccgttctag gtattgatac cttcggagcc tctgccccag caccaaaagt
    34141 attggcagaa tatggcttta ctgtagaaaa tcttgtaaaa gttgttcgaa acttgaaata
    34201 atcctaaaaa tcagggcgca agctctggtt tttcttacca gaaaagtaag gtacaatctt
    34261 gtaaaagtag ccgaaatttg atatagtagt cctatgtaaa agacaaagga gaagacaatg
    34321 aatccaaatt atacattttt aatcatgcta gcccttatgg tgggcttgat attctttacg
    34381 caacgctctc aaaagaaaca agcacaaaaa cgtatggaaa gcttaaataa actacaaaaa
    34441 ggctatgaag tgattacaat cggtggactt tatggaacag tcgatgaagt agatacggag
    34501 aagggaacaa tagttcttga cgtagatgga gtttacttga cttttgaact agctgctatc
    34561 aagacagtat taccgctgaa agaaacagct tcactagaag gcgcaattga aaaataagac
    34621 gggatcacga actcccgttt ttctataaaa gaaaggaaat gggatgaaaa aattagtctt
    34681 tgtctgtcta ggaaatattt gccgtagccc tatggccgag tttgttatga aatcaatgac
    34741 agataactac gaaatccaaa gtcgagcaac ttcctcttgg gaacatggca atccgattca
    34801 taaggggact cagggaattt ttcaacagta tgagattcct tatgacaaga acaagacatc
    34861 gcttcagatt agtaaggaag attttgaagc ctttgattat attatcggaa tggacgcttc
    34921 aaatgtttcc gacttacgtc agatgtgtcc agtagactgt caagataaga tttactcatt
    34981 ttcatctgaa agtgttccag atccttggta tacaggagat tttgaggaaa cctatcagcg
    35041 tgtccaggag ggttgtaaag tttggttaga acgtttagaa aaggagagtg aagatggaaa
    35101 atcttaagag tttttatgaa aagtatcgtg tctatctgac tcgtccacgt ttagagcttt
    35161 tagcagtagt taccattgtt ttttgtgctg tactcgtctt ttttctaaat attcccggaa
    35221 aaggtattct aaaactcgat aatggaacga ttgtttacga cggcagtctt gtccgtggta
    35281 aaatgaatgg acaaggtacc attaccttcc aaaatggaga ccaatataca ggtggcttca
    35341 acaatggagc cttcaatgga aaaggtactt ttcaatctaa agaaggttgg acctacgaag
    35401 gtgattttgt aaatggtcag gctgaaggaa aaggaaaact aacaacagaa caagaagttg
    35461 tttatgaagg gacttttaaa caaggcgttt ttcaacaaaa ataaagcctc cttatcaaag
    35521 gaggtattgt tagaagtttt aagtaagcgc ttacttgcaa atccttttct ttccaaatcc
    35581 ctcttccaag caagtttgtg aaataaaaaa tatttgaaat aaatttcaca aacttcaaag
    35641 ataaaacctg ataagaaaag aaaatgagaa aagtttcaca agagtttaaa aattctttgt
    35701 gaaatgttta tgaaactgtt tacaaagttg aaataatgcg ttataataaa cttgtgaaaa
    35761 aattaacaaa ggatatcgat ccttgaaagc tatggaggaa aatatggctg ataaaaaaac
    35821 tgtgacacca gaggaaaaga aactcgttgc tgaaaaacac gtagatgagt tggttcaaaa
    35881 agctctagtt gcccttgaag aaatgcgtaa attggatcaa gagcaagttg actacatcgt
    35941 tgccaaagca tcagtagcag ctttggatgc ccacggagaa ttggctttac atgcctttga
    36001 agaaacagga cgtggtgtat ttgaagataa agcaactaag aacttgtttg cctgtgaaca
    36061 cgtagtaaac aacatgcgcc acactaagac agttggcgtt atcgaagaag acgatgtaac
    36121 aggattgact cttattgctg aaccagttgg tgttgtttgt ggtattactc caacaacaaa
    36181 cccaacatca acagcaatct tcaaatcatt gatttcattg aagacacgta acccaatcgt
    36241 ctttgccttc catccatcag cacaagaatc atctgctcat gcagctcgta tcgtccgcga
    36301 tgcagctatc gcagctggtg ctcctgaaaa ctgtgtgcaa tggattagtc aaccatctat
    36361 ggaagcaaca agtgccctta tgaaccacga aggtgttgcg acaatccttg caacaggtgg
    36421 taatgccatg gttaaggcgg cttattcatg tggtaaacca gctcttggag taggtgccgg
    36481 aaacgttcca gcttatgttg aaaaatcagc aaacattcgt caagcagcac acgatatcgt
    36541 catgtctaaa tcatttgata acggtatggt ctgtgcatct gaacaagcag ttatcattga
    36601 taaagaaatt tacgatgaat ttgtagcaga gttcaaatct taccacactt actttgtaaa
    36661 caaaaaagaa aaagctcttc ttgaagagtt ctgcttcggc gtcaaagcaa acagcaaaaa
    36721 ctgtgctggt gcaaaattga acgctgacat cgttggtaaa ccagcaactt ggattgcaga
    36781 acaagcagga tttacagttc cagaaggaac aaacattctt gctgcagaat gtaaagaagt
    36841 tggcgaaaat gagccattga ctcgtgaaaa attgtcacca gttatcgcag ttttgaaatc
    36901 tgaaagccgt gaagatggta ttactaaggc tcgtcaaatg gttgaattta acggtcttgg
    36961 acactcagca gctatccaca cagctgacga agaattgact aaagaatttg gtaaagctgt
    37021 taaagctatt cgtgttatct gtaactcacc ttctactttt ggtggtatcg gggacgttta
    37081 caatgccttc ttgccatcat tgacacttgg atgtggttct tacggacaca actcagttgg
    37141 ggataacgtt agtgccatta acctcttgaa tatcaaaaaa gtcggaagac ggagaaataa
    37201 catgcaatgg atgaaacttc cttcaaaaac atactttgaa cgtgattcaa ttcaatacct
    37261 tcaaaaatgt cgtgacgttg aacgtgtcat gatcgttact gaccatgcca tggtagagct
    37321 tggtttcctt gatcgtatca tcgaacaact ggaccttcgt cgcaataagg ttgtttacca
    37381 aatctttgcg gatgtagaac cggatccaga tatcacaact gtaaaccgtg gtactgagat
    37441 tatgcgtgcc ttcaaaccag ataccatcat cgcactcggt ggtgggtctc caatggatgc
    37501 tgccaaagta atgtggctct tctacgagca accagaagtg gacttccgtg accttgtcaa
    37561 aaaattcatg gatatccgta aacgtgcctt caagttccca ttgcttggta agaagactaa
    37621 attcatcgcg attccaacta catctggtac aggatctgaa gtaacaccat ttgccgttat
    37681 ctctgataaa gcaaacaacc gtaaataccc aatcgctgac tactcattga caccaactgt
    37741 ggcaatcgta gatcctgctt tggtattgac agttccagga tttgttgctg ctgatactgg
    37801 tatggacgta ttgactcacg cgacagaagc atacgtatca caaatggcta gtgactacac
    37861 tgatggttta gcacttcaag ccattaaatt ggtctttgaa aatctcgaaa gctcagttaa
    37921 gaatgcagac ttccactcac gtgagaaaat gcataacgct tcaacaatcg ctggtatggc
    37981 ctttgccaat gccttcctag gtatttctca ctcaatggcc cataagattg gtgcgcaatt
    38041 ccacacagtt cacggacgta caaatgccat cttgcttccg tacgttatcc gttacaacgg
    38101 tacacgtcca gctaagacag caacatggcc taagtacaac tactaccgtg cagatgaaaa
    38161 ataccaagat atcgcacgca tgcttggact tccagcttct actccagaag aaggggttga
    38221 atcttacgca aaagctgtct acgaactcgg tgaacgtatt gggatccaaa tgaattttag
    38281 agaccaagga attgacgaaa aagaatggaa agaacattct cgtgaattag ccttcctggc
    38341 ttatgaagac caatgttcac cagctaaccc acgtcttcca atggtagacc atatgcaaga
    38401 aatcatcgaa gatgcatact atggctacaa agaaagacca gggcgccgta aataattgtt
    38461 tatcagtcta gaagcaagac aaaaactcaa tttgagggaa agaaccagta atttttctat
    38521 gataaaaggc atcctatcaa ggtttttgaa tacctgatag gatgcctttt tatgatgttg
    38581 aggccttttt gccctttttg aaaaactaga atagaaacaa aatatatagt aggttgaaac
    38641 tagaatagta cacatctgct tctaaaacat tgttagaatt c
//
LOCUS       DAVKDZ010000010        38539 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000010 DAVKDZ010000000
VERSION     DAVKDZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38539
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.010"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            72..899
                     /gene="xth"
                     /locus_tag="VWZ30_000521"
     CDS             72..899
                     /gene="xth"
                     /locus_tag="VWZ30_000521"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU9334321.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            911..1054
                     /locus_tag="VWZ30_000522"
     CDS             911..1054
                     /locus_tag="VWZ30_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9334322.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            1165..1293
                     /locus_tag="VWZ30_000523"
     CDS             1165..1293
                     /locus_tag="VWZ30_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEU9334323.1"
                     /translation="MKANYPIYRPLTKRKFRVSDHYIYPHEFAVLDPNGYFLRFSE"
     regulatory      1677..1772
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            2172..2753
                     /locus_tag="VWZ30_000524"
     CDS             2172..2753
                     /locus_tag="VWZ30_000524"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU9334324.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAEKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            2753..4015
                     /locus_tag="VWZ30_000525"
     CDS             2753..4015
                     /locus_tag="VWZ30_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEU9334325.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(4102..4968)
                     /locus_tag="VWZ30_000526"
     CDS             complement(4102..4968)
                     /locus_tag="VWZ30_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnII"
                     /protein_id="HEU9334326.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
                     AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFY
                     SGSGSKLNETARSYKMIAEETKAIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNI
                     NDLEHNILKNLK"
     gene            complement(4955..5725)
                     /locus_tag="VWZ30_000527"
     CDS             complement(4955..5725)
                     /locus_tag="VWZ30_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU9334327.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            complement(5751..6605)
                     /locus_tag="VWZ30_000528"
     CDS             complement(5751..6605)
                     /locus_tag="VWZ30_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEU9334328.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELMPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYLNNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDVFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            complement(6762..7166)
                     /locus_tag="VWZ30_000529"
     CDS             complement(6762..7166)
                     /locus_tag="VWZ30_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU9334329.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERQVRI"
     gene            complement(7236..8717)
                     /locus_tag="VWZ30_000530"
     CDS             complement(7236..8717)
                     /locus_tag="VWZ30_000530"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9334330.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSRKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEHVGISLD"
     gene            complement(8736..9914)
                     /locus_tag="VWZ30_000531"
     CDS             complement(8736..9914)
                     /locus_tag="VWZ30_000531"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU9334331.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPATISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLERDYEVTGLELDALVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            10078..11085
                     /gene="galR"
                     /locus_tag="VWZ30_000532"
     CDS             10078..11085
                     /gene="galR"
                     /locus_tag="VWZ30_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEU9334332.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELIFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(11131..12123)
                     /locus_tag="VWZ30_000533"
     CDS             complement(11131..12123)
                     /locus_tag="VWZ30_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9334333.1"
                     /translation="MDLRPQIIEADDVIIRVVRACVCGSDLWRYRNPETKAGHKNSGH
                     EAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGSCDNHIGNNLGGDFQA
                     EYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHAARVANVQKGDKVVVI
                     GDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVVAERGQEGITKVREIL
                     GGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRALGSTFMQNISVAGGAA
                     SATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDERKTIKSMIVIE"
     gene            complement(12158..12511)
                     /locus_tag="VWZ30_000534"
     CDS             complement(12158..12511)
                     /locus_tag="VWZ30_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9334334.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(12755..13645)
                     /locus_tag="VWZ30_000535"
     CDS             complement(12755..13645)
                     /locus_tag="VWZ30_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU9334335.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGIFGSSAVLADSVHDLVDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINMLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            13778..14314
                     /locus_tag="VWZ30_000536"
     CDS             13778..14314
                     /locus_tag="VWZ30_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9334336.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLQRELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(14481..15287)
                     /locus_tag="VWZ30_000537"
     CDS             complement(14481..15287)
                     /locus_tag="VWZ30_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU9334337.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKTIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(15632..17152)
                     /locus_tag="VWZ30_000538"
     CDS             complement(15632..17152)
                     /locus_tag="VWZ30_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEU9334338.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLAALIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLVQKEKENLVIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGKTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMEPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAEQVAKEFLQEQGLLKK"
     gene            complement(17145..17873)
                     /locus_tag="VWZ30_000539"
     CDS             complement(17145..17873)
                     /locus_tag="VWZ30_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9334339.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            complement(17888..18652)
                     /locus_tag="VWZ30_000540"
     CDS             complement(17888..18652)
                     /locus_tag="VWZ30_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU9334340.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     CIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(18656..19099)
                     /locus_tag="VWZ30_000541"
     CDS             complement(18656..19099)
                     /locus_tag="VWZ30_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9334341.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(19781..20077)
                     /locus_tag="VWZ30_000542"
     CDS             complement(19781..20077)
                     /locus_tag="VWZ30_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9334342.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(20099..21163)
                     /gene="pepA"
                     /locus_tag="VWZ30_000543"
     CDS             complement(20099..21163)
                     /gene="pepA"
                     /locus_tag="VWZ30_000543"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEU9334343.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKLDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            21564..22265
                     /gene="galE"
                     /locus_tag="VWZ30_000544"
     CDS             21564..22265
                     /gene="galE"
                     /locus_tag="VWZ30_000544"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9334344.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNASGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            22688..23647
                     /locus_tag="VWZ30_000545"
     CDS             22688..23647
                     /locus_tag="VWZ30_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9334345.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWVLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            23637..24593
                     /locus_tag="VWZ30_000546"
     CDS             23637..24593
                     /locus_tag="VWZ30_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU9334346.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLNCLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            24590..25342
                     /locus_tag="VWZ30_000547"
     CDS             24590..25342
                     /locus_tag="VWZ30_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9334347.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            25438..26403
                     /locus_tag="VWZ30_000548"
     CDS             25438..26403
                     /locus_tag="VWZ30_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9334348.1"
                     /translation="MKTSLKLYFIALVASFLLLLGACSTNSSTSQTEPSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(26628..26870)
                     /gene="yidD"
                     /locus_tag="VWZ30_000549"
     CDS             complement(26628..26870)
                     /gene="yidD"
                     /locus_tag="VWZ30_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU9334349.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMSLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(26870..27592)
                     /locus_tag="VWZ30_000550"
     CDS             complement(26870..27592)
                     /locus_tag="VWZ30_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9334350.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(27579..28148)
                     /locus_tag="VWZ30_000551"
     CDS             complement(27579..28148)
                     /locus_tag="VWZ30_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEU9334351.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(28167..28895)
                     /locus_tag="VWZ30_000552"
     CDS             complement(28167..28895)
                     /locus_tag="VWZ30_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU9334352.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(28895..29635)
                     /gene="xerD"
                     /locus_tag="VWZ30_000553"
     CDS             complement(28895..29635)
                     /gene="xerD"
                     /locus_tag="VWZ30_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU9334353.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGGKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(29626..30087)
                     /locus_tag="VWZ30_000554"
     CDS             complement(29626..30087)
                     /locus_tag="VWZ30_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9334354.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDTEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(30084..30605)
                     /locus_tag="VWZ30_000555"
     CDS             complement(30084..30605)
                     /locus_tag="VWZ30_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU9334355.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(30581..31552)
                     /locus_tag="VWZ30_000556"
     CDS             complement(30581..31552)
                     /locus_tag="VWZ30_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEU9334356.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(31549..32343)
                     /gene="racE"
                     /locus_tag="VWZ30_000557"
     CDS             complement(31549..32343)
                     /gene="racE"
                     /locus_tag="VWZ30_000557"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU9334357.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            complement(32550..32798)
                     /locus_tag="VWZ30_000558"
     CDS             complement(32550..32798)
                     /locus_tag="VWZ30_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEU9334358.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(33054..34679)
                     /gene="treC"
                     /locus_tag="VWZ30_000559"
     CDS             complement(33054..34679)
                     /gene="treC"
                     /locus_tag="VWZ30_000559"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEU9334359.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDTSENAGFSTGTPWLKVGKSYKDINVENEIKGLIFTFY
                     QDLIRLRKEMPIISEGNYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(34857..36824)
                     /gene="treP"
                     /locus_tag="VWZ30_000560"
     CDS             complement(34857..36824)
                     /gene="treP"
                     /locus_tag="VWZ30_000560"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEU9334360.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTINRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKIEGDTNLQAEFVAQEEAEFVSHEPVELTSVEII
                     SPLTGQVKELSQATDPVFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVIVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATITGTYPTTIQAGASLMVATRI"
     gene            37009..37719
                     /gene="treR"
                     /locus_tag="VWZ30_000561"
     CDS             37009..37719
                     /gene="treR"
                     /locus_tag="VWZ30_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEU9334361.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVGRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            37874..>38539
                     /locus_tag="VWZ30_000562"
     CDS             37874..>38539
                     /locus_tag="VWZ30_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1167A family transposase"
                     /protein_id="HEU9334362.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQI
                     PRIINQKIAQKLIEKMSMTDIAHQLSISTSTVIRKLNDFHFEHDFSRLPKIMSWDEYA
                     FTKGKMSFIAQDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQVKIITMDMFSPYYD
                     LAKQ"
BASE COUNT        11292 a         8886 c         7117 g        11244 t
ORIGIN      
        1 actatacgtt ggggaaatag atcttttttt ggtataataa aatctataat ctgaatgaaa
       61 aaggtaactt tatgaaactt atctcatgga atattgattc cctaaacgct gccctaacta
      121 gtgactcagc tcgtgccaaa ttgtcccaag aagtcctaca aaccttggtc gctgaaaatg
      181 ctgatatcat tgctatccaa gaaaccaagc tttctgccaa aggacctaca aagaaacacg
      241 tggaaatttt agaagaactc ttcccaggct acgaaaacac gtggcgttct tcccaagagc
      301 ctgcccgtaa aggctatgct ggaaccatgt tcctttataa gaaagaactt acacctacta
      361 tcagcttccc agaaatcggt gccccttcta ccatggactt ggaaggtcgt atcatcactc
      421 tagaatttga tgcatttttc gtaacccaag tttacactcc aaacgctggt gacggtctca
      481 aacgcttgga agaacgccaa gtctgggatg ccaaatatgc tgagtatttg gctgaactag
      541 acaaagaaaa accagtcctt gcgaccggtg actacaacgt agcccacaat gaaatcgacc
      601 ttgcaaatcc tgctagcaac cgccgttcac ctggatttac tgacgaggaa cgtgctggat
      661 ttaccaacct tttggcaact ggatttacag acaccttccg ccatgttcat ggcgatgttc
      721 ctgaacgcta cacttggtgg gcacaacgca gcaaaacttc taaaatcaac aatacaggct
      781 ggagaatcga ctactggctc acaagtaacc gtatcgctga caaggtcact aaatctgata
      841 tgattgactc aggtgctcgc caagaccata caccgattgt cttggaaatt gacttgtaag
      901 gattttctat atggactaca atgcggtcat tcccgagttg cttgtatcga atatcgaaca
      961 gtcccggtcc ttctactgtg gtttactggg ttttaggatt gaataccaac gtccagaaga
     1021 aaactttctc tttctcttga agagtgtcaa ctaatgttag aggagggaac aaaggatcaa
     1081 ctagctgaac tgacctatcc atttggtcgt ggagtcaatc tctcctttgg cataaaggat
     1141 gtttcaaaac tctattaaag agtgatgaag gcaaactatc ctatttatcg tcccttgacc
     1201 aagagaaagt ttcgtgtcag tgatcactat atctatcctc atgaatttgc agttttggat
     1261 ccaaatggct attttttaag atttagcgag tagaataata aaaattggga aaagtcttta
     1321 aagttagaaa aaggcatatt gtcaggtgtt taaaaacttg atagtatgcc ttttatgatg
     1381 gaaaaatctt ctaatactct tcgaaaatct cttcaagcca catcaacgtt gccttaccgt
     1441 aggtatatga tactgactct gtcaattcta tccacaacct caaaacagtg atttgaggtt
     1501 gtggatagct tcctagtttg ctctttgatt ttcatttagt ataaatgtta gcttacaacg
     1561 gagttttttg ttcgccctct cttctgtttg ttcgtttttt aaaccaaaac actaacaaaa
     1621 tagttgattt ctaaagttat ttagtgtaaa ataaaaacat tggctcaagc ctatttaaaa
     1681 ttgaatatcg ttttacttgt ttatgtcgtg aattggcacg acgtttctac aaggtgccgg
     1741 aacacctaac aataagtaag tcagcagtga gatatggccg tttttgccat gcctatttgg
     1801 gggacttact tagagattaa gtaggtcttt tttgaatatt tttaatattt cccttaatta
     1861 gtgcggacgg gaggtgaaat tatcctgtga ataatttcag gaatccaggg aatttcatcc
     1921 ctaatttttg agcccctagc tctttcattt ctaggcttag gctaaaatag ttccccgaac
     1981 tattttactc tcaaaaatcc cgtcatagac aataacaaga ttattgtcta tgataccact
     2041 acatcaggaa atatttgata agactcgctt cgctcgtaat agttttatca aatatatagt
     2101 attagcaaaa gggatcacta tagaaatatt taaacaacaa gtaaattaac ttttagactt
     2161 taggaggtct tatgaaatta ttagaagagc gcatcctcaa ggatgggcat atcttgggtg
     2221 ataacatcct caaggtagat tcctttttaa cccaccaagt tgactttagc ttgatgcgag
     2281 agattggtaa ggtttttgcg gaaaaatttg ctgctactgg cattaccaag gtcgtaacca
     2341 ttgaagcgtc gggtattgcc ccagccgttt ttacagctga agccttaaac gttcccatga
     2401 ttttcgccaa aaaagctaag aacatcacca tgaacgaagg catcttaact gctcaagtct
     2461 actcctttac caaacaggtg accagcaccg tttctatcgc tgaaaaattc ctctcaccag
     2521 aggacaaggt tttgattatc gacgatttcc ttgctaatgg ccaagctgct aaaggcttga
     2581 ttcaaatcat cgaacaggcc ggtgccacag tccaagctat cggtatcgtg attgagaaat
     2641 ccttccaaga tggtcgtgat ttgcttgaaa aagcaggcta ccctgtccta tcacttgctc
     2701 gtttggatcg ttttgaaaat ggtcaggtcg tatttaagga ggcagatctc taatgcaaac
     2761 tcaagaaaaa cactcgcaag cagccgttct tggcttgcag cacttactag ccatgtactc
     2821 aggatctatc ctggttccca tcatgattgc gacagccctt ggctattcag ctgagcagtt
     2881 gacctacctg atttctacag atatcttcat gtgtggggtg gcaaccttcc tccaactcca
     2941 actcaacaaa tactttggga ttggactccc agtcgttctt ggagttgcat tccagtcggt
     3001 cgctcccttg attatgattg ggcaaagcca tggtagtggc gctatgtttg gtgcccttat
     3061 cgcatctggg atttacgtgg ttcttgtttc aggcatcttc tcaaaagtag ccaatctctt
     3121 cccatctatc gtaacaggat ctgttattac cacgattggt ttaaccttga tccctgtcgc
     3181 tattggaaat atgggaaata acgttccaga gccaactggt caaagtctct tgcttgcagc
     3241 tattactgtt ctgattatcc tcttgatcaa catctttacc aaaggattta tcaagtctat
     3301 ctctattttg attggtctgg ttgttggaac tgccattgct gctactatgg gcttggtgga
     3361 cttctctcct gttgcggtag ctccacttgt ccatgtccca actccactct actttgggat
     3421 gccaaccttt gaaatctcat ctattgtcat gatgtgtatc atcgcaacgg tgtctatggt
     3481 tgagtcaact ggtgtttatc tggccttgtc tgatatcaca aaggatccaa tcgacagcac
     3541 gcgccttcgc aacggttacc gcgcagaagg tttggccgta cttctcggag gaatctttaa
     3601 caccttccct tacaccggat tttcacaaaa cgttggtttg gttaaattgt caggcatcaa
     3661 aaaacgcctg ccaatctact acgcagctgg tttcctggtt ctccttggac tgcttcctaa
     3721 gtttggtgcc cttgcccaaa tcattccaag ctccgtcctc ggcggtgcca tgctggtgat
     3781 gtttggtttt gtatctattc aagggatgca aatcctcgcc cgagttgact ttgctaacaa
     3841 tgaacacaac ttccttatcg cagctgtttc aatcgctgca ggtgtcggtc tcaacaacag
     3901 taatctcttt gtcagcatgc cgacagcctt ccaaatgttc ttctcaaacg gaatcgtcgt
     3961 agccagccta ctcgccattg tcctcaatgc cgtattaaat cataaaaaga aataagaaaa
     4021 agaggtgcga gcctcttttt tgtttatcac aaattgatta taaatacaat caatcccaat
     4081 tcccatctaa ttgaattgga cctatttcaa attctttaaa atattatgtt ccaaatcgtt
     4141 aatattatat aagaatggta aaatatcaaa tgtttctcgt aaattattct tagccttata
     4201 ccaaccttgt ccatcagtaa tccacataaa ctcaacatta ggaatagctt tagtttcttc
     4261 agcaatcatt ttgtaagatc tagccgtctc atttaatttt gaaccacttc ctgaataaaa
     4321 attaacctca atcagataaa gcacttgttc attttttatg acaaaatcaa aacgtttcac
     4381 agtattccca tcattcgtaa tagcagataa atcgacagaa aaaatttcct ctatttcatt
     4441 ttgttcaatc tctttaaata aattctctcc taaaatatac ccttcagctt caagatagct
     4501 ttgaacaata ttttccatcg catctccagt ccgatttttg cgcccattag tatccatacc
     4561 aacctcaaca cctgtaacgt agtcaactaa atttgatact aaatgatttt gcaataaatc
     4621 aaatatacct gatttttcca aaaacatagt atattcttca atactatagt ttcgtttact
     4681 aaaatcaaaa tagaaatctt ttatcggatc cttaacaata atcgtatctc tcaaacgttt
     4741 agcaataagt agaggcacaa ctttcaaaat ttctggataa ttttgatata agtctaaaaa
     4801 atcctcttgt atatttttac taccaataag actattcata atatttagag caacttttat
     4861 actagataca tttttataaa ccttaggaaa atctgtataa taagtccagt cagctactgt
     4921 gtcggtcatc gttgacaacc actcatcaaa atttctagtt tgtttcattt tctgcctcca
     4981 acctcttcct tgcaattttt aaatattctt tttcagcatc gatacctata aatcttctac
     5041 ctaaccgctt cgcaacaaca cccgtagtgc cactaccaac aaatgggtct agaatatagt
     5101 caccctcttt agtagaggct aaaataatac gttctaacaa atactctggt ttttgagttg
     5161 gatgtttccc agcccatttt tcaactttct ttgttaaaga accggtccag acatctttca
     5221 tttgttttcc atcattcaat tctttcatta aatcataatt gtagtaatga cgagcttttt
     5281 tatcattttt tctggcccat aaaatggttt cagtagaatg ggtaaaataa cgacaagata
     5341 aattgggggc agggtttgtt ttctgccaag taatattatt cagaatttta aaaccttctt
     5401 gttctaatgc cattccaact gagtatatgt tgtgcaaact acctgaaatc cataccgtcc
     5461 cattaggctt cagaacttct tttgctaggc gaatccattt acgattaaac tcatgttttt
     5521 cttcgaatga agaaatttta tcccaatctc ctttatcaac agaaactact tgtcccccag
     5581 aattagatat tccaccatta cttaaaaaat aaggtggatc agcaaaaatc atatccatac
     5641 tttctggttt catttttgat aagaacttga acgtatctga atgaacaaga atcatcttat
     5701 ttttattgta gtatggtttt gtcataagaa cacctccata cttatattcg ttatttttca
     5761 taatttgtga ctataatttc agaaattttt cctcgacttg aagattttgc tccattagtt
     5821 cgggtagctt caacataatg tatattaaaa tccttataca actcctctac taaagcacta
     5881 gaagaatttg ataacataac ataagctcct gtatcactta atctcttaaa gacatctctt
     5941 aatcttactt gatctgcaaa agagaatccc tcatgagtat aagacgtaaa tgcactcgtc
     6001 tcagacaatg gaatatatgg agggtcaaaa tacacaaaat ctcctgttcg aacatctaca
     6061 attgcctttt caaaatctcc cactttaatt tctagttgat tgttatttag ataaactgaa
     6121 atagcagata tcaattcctc atcaacaatt ttaggattct tataacgtcc atatggaaca
     6181 ttaaattggt tcttagaatt cacacgatat agaccattaa agttcactct caacatatat
     6241 agaatacgtg cagctctttg tacttcggac atcatatcta ttctttcatc acgatctgca
     6301 gaacgtaaat ctaaataata ttcttttgaa ttatattcct gatgaacttt caaaatttca
     6361 atcaattctt gaggattgtc cttaatttgt tgatagcaat ttattagttc agcgttaaaa
     6421 tcattaataa ctgcatcttt aggagccaaa tcaaaaaata aagctccacc tccaacaaaa
     6481 ggttcgaaat acctgttata ggttttaggc attaattctc taataacagg caataattgt
     6541 cttttaccac ctgtccattt cgtgaacggt tgtaaagtaa ctttctttat ttcttttatc
     6601 ttcatgcttt taattatacc atagaaatta aaacacaact cttgtatttt ctaccacatt
     6661 actaaatttt ctattttaga attaaatcat atcaatatca aaaaatgagt aaggcaaatc
     6721 ttataaattc caaaattcac ctcactctat taaattactc tttatatcct tacctgtctt
     6781 tcttctgacc gctggcctgt gacaaacatg gtaaaggttg ctttacagac atttctgccc
     6841 tcttgattgg tgatatccac atccatcaca caggttgtac ggccttgatg gacacattct
     6901 cctttaatgg tcaatacatc gtcgagtttt cctgccttaa ggtagttgat agaggattgg
     6961 agtgtcaccc catcaagtcc cagcgagata accaccaaac cactgatttg gtcacatagc
     7021 gtgaatagat agccaccatg ggcattgcca tagtagttga gcgacgagtt cactactttg
     7081 gtcgtcacca caacgtgacc atctctcatt ttttcaattt cgtaattttc aaaggcagat
     7141 atagcgtcaa aatgaaagtc tttcatcgtt tcctcctgat atttcaaacg acactatgat
     7201 actacttttt ataggactgt gcaaggagaa agctcctagt ctagtgaaat tccgacatgt
     7261 tccacaaagc gcataaaggc tgtctgccct tgttctgtct gcttgtagac tcctgcatcc
     7321 tcaagtacac gcacaaagat agcacccaca gagtccttga cgattgcaag ggctttttct
     7381 ttatccgtta ggtctggatg ttgggatttg agttggtctg cccactcctg atgataatcg
     7441 gcaactgtaa cagcttctcc tacaagatag ctagcgactt gctccacttc ttctttcaga
     7501 cgtggtggca agattgccaa gcccatgacc tcaatcaagc cgatattttc cttcttgata
     7561 tgttggacat ccttgtgggg atgatagatg ccatcaggat gctctgctga agtctgattg
     7621 tctcgcaaga ccaagtccaa ctcaaactgt ccatcacgct tacgcgctat tggtgtgatg
     7681 gtgtgatgcg gtgtcccgtc tgtctctgct aaaatctgca cactagaatc tgaatactga
     7741 cgccattcct ggaaaatctt atcagctaag ttaatcaaat cctctttgga atccgaagtc
     7801 aagcgtagga cagacattgg ccacttgata attccagcct tgacctgctc aaaaccagca
     7861 aatcggaagg tcttttgcaa gggagccaat tccataggaa atacgtgacg gcctccctga
     7921 taatgatcat gagttagaat agagcccccc acaatcggca ggtcggcatt agatccagca
     7981 aaatatcctg gaaactgctc tacgatagcc aacagacgtt caaaactctg acgactaatg
     8041 gccatgggac gatgctggcc atctaaaaag atacaatgct cattaaagta agcatagggc
     8101 gaatactgga acccccattc ctgaccaacc atttcaaaac ggataatccg gtggttgctg
     8161 cgagctgggt ggttgacccg accatggtaa ccctcattct ctaaacaaag ttgacactga
     8221 ggataattac tagcttgcac caacttggct accgcaatct ctttcggatc cttttcaggc
     8281 ttagagagat tgatggtaat ttcaagttct ccataatcag atggaacacg ataagcgata
     8341 tttttagcaa tggccttgag tttgatgtag tcatttttcc gactgagttg ataaaaatcc
     8401 tctatcgctt gctcaggaga gtaggcatag gcttcccaga agtcacgatt gacctgactc
     8461 ggacaaggag tcaccaagtc catcagttca gtaccaagga tttcacgcgc agtctgacta
     8521 tcctcaatcg tctctaatcg aacggcttcc tcaactagct ggtccttgag gtcaatcaat
     8581 ttatccagat tggtctcaac ttccaaaaca ccttctccca ctcgtgccaa aacacggttt
     8641 gtcaggtaga ttcgatccat ttcctcaaat gaactttcag aaatgacatg tgttacaaat
     8701 ttatctacta aggtcactat agagcctcct tttgactagt caaggacgcg agtgccacct
     8761 gcaacttcag cgatatagaa gcttggagcg tatccaacta cttcctcgta gtgtttgcct
     8821 acagcttcct taaaggcctc aacagtatct ttttgaacca aggcaatggc acagccacca
     8881 aaaccagccc ctgtcatacg agcaccgaga actccttctt gtgcccaagc tgtgtgaaca
     8941 agagcatcca attccaaacc agttacttca taatcacgct ccagagaaac gtgtgacgca
     9001 ttcatcaaac gaccaaatgt ttccaaatca cctgcttgaa gggctgcttg agctttaagg
     9061 gtacgttggt tttcaagcac agcatggcga gcacgtttca aacgattttc atctttaatc
     9121 aaatagctat attggtcaac ggcccactcg tccaattcac ccagggtctg aatatccaag
     9181 gcaacttgca attcttccac tgctttttca cactcagcac gacgttcatt gtatttagaa
     9241 tccgccaatt cacggcgttt gttggtgttc atgataacaa cgacattgtc cttcaaatca
     9301 agtggcacca aatcatactc taaggtgttg gtatcaaggt aaatagcacg ttgatcagcc
     9361 cccataccaa tagcaaactg gtccatgata ccagagttga ctccgataaa gttattttct
     9421 gtttgttttc cgattttaac caaatccaaa cgctctaatt ttaaatcaaa gagatgctct
     9481 gccactaccc ctgtcaagag ctccaaggat gctgaagaag acaaaccagc accatttggg
     9541 atattcccat aaacataaaa atcaaaacct ttgtcaatca cgtgcccagc ttcttgcaag
     9601 aaatgaagga caccttttgg ataattggtc cagttgtgct ctttttcaaa cttgaggtca
     9661 gcgagaggca cttcgataat gcccttgtcc tcaaagttag ctgagtagaa acgcaaaact
     9721 tggtcgtcac gcttacgagc tgcaccgtaa gttcccaagg aaatagtagc aggaaaaacg
     9781 tgcccaccgt tgtagtctgt gtgttcacca atcaaattga tacgacctgg tgaaaagaaa
     9841 gtttggtctg cttcttgacc aaaaacagca agaaagtctt tgcgaagagt ttcagcagta
     9901 agatgttgtg tcatatgatt tctcctttga ctgtccgtta agccacctta acgtgtaatc
     9961 gttttcttct attgtatcca aggaatttcc caaggtcaat cctttttact aaaattttag
    10021 taaaatatca gtaaaaagaa gaaaaatatg atatactaac ctaatcaagg aggatttatg
    10081 gctaccttaa aagacattgc acagctagcc tctgtctcta tcgcgaccgt atcccgtgtc
    10141 ctcaaccgcg accagagcct atctgttaca gaagaaacca gacaccgtat tttaaccgtt
    10201 gctgaagagc tgggctacac caagcacctc aagacaggcg agtcccataa acccaagcaa
    10261 aagattgcca ttattcaatg ggtcagcgaa caaggggagc tagacgacct ctactactac
    10321 cagattcgcc taggaataga aaaaagagcc caagagttgg actacgatat cttgcgctac
    10381 tttaatgacc acccttttac cctaagcgag gaagtgattg ggattctctg catcggaaag
    10441 tttagtcgag ctcagatttc tgcctttgaa gaataccaaa agcctcttgt atttctagac
    10501 agcgatacac tttccctggg acatacctgt attatcacgg atttttacac tgctatgaaa
    10561 caggttgtcg attatttcct cagccaagga atggaccgta tcgggattct aacaggcctt
    10621 gaagaaacaa ctgaccaaga agaaatcatt gaggataagc ggctggaaaa tttcagaaac
    10681 tacagtcaag caaaaggaat ctatcatgat gaactgatct ttcaaggaaa ctttactgcc
    10741 cagtctggct atgacttgat gaaggaagcc attcagaact tgggagacca actcccacca
    10801 gcatttttcg cagccagcga tagtttagcc atcggtgccc ttcgtgcgct ccaagaagct
    10861 ggaatcagcc taccagaccg tgtcagcctc atttccttta acgacaccag cctgaccaag
    10921 caggtttatc ctcccctctc cagcatcaca gtttatactg aagaaatggg gcgagcaggt
    10981 atggatattc ttaacaagga agtcctccac ggtcggaaaa tccctagcct gaccatgctg
    11041 ggaaccagac tgacattaag agaaagtacc ctaaatcaag aataggataa cataaaaaac
    11101 gaatagagtt ctaaaactcc tattcgtttt ttattcgatt acaatcatag acttaattgt
    11161 cttacgttca tccatatctt tataggcttg gtcgatatct tccagtttat aacttgaagt
    11221 aaagacgcga cctggattga tatcaccatc aaggacggct tttagtaaaa attgcttatc
    11281 gtatgttgta gcagaagctg ccccacctgc tacagagata ttttgcataa atgtcgaacc
    11341 aagagcacga ttattatagt gtgggactcc tacaaagccc atacgccctc cattatgaag
    11401 aacacctagc gcctgttcta tagcagcctc cgtaccaaca cattcaagtg ctgcatctgc
    11461 tcctccaccg aggatttcac gcaccttggt aattccttct tgaccacgtt ctgcaacaac
    11521 agctgtcgca cctgactcca tagccatctt ttgacggtct tcatgacggc tcataaggat
    11581 aatttgtgat gctccacgca tcttagccgc gatgacagca cattgaccaa cagccccatc
    11641 accgataaca acaaccttgt cccctttttg aacatttgca acacgcgccg catgatagcc
    11701 tgtcggcatg acatctgcaa gagtcaaaag ggacttgagc atcccttctg tatagtcaga
    11761 aggttgacca gggattttaa ccagcgccca gtttgcatag tggaagcgaa tatattctgc
    11821 ctgaaaatca ccccccaaat tattgccaat atgattgtcg caagaaccgt caaatccagc
    11881 aagacaggca tcacactcac cacatccatg tgtaaaaggg acaatcacaa aatcacctgg
    11941 tttcaccgtc gtaatggctt ccccagcttc ttcaacaatc ccaatcgctt cgtgtccact
    12001 atttttgtgt ccagctttcg tttctggatt acggtacctc cataaatctg aaccacaaac
    12061 gcacgcacga accacacgaa taatcacatc atccgcttct attatttgcg gacgcaagtc
    12121 caacctgacc tgcctttgta tatactgctg atttcattta aaattttcct tccttataaa
    12181 gtttaatttt gagatttaaa cgatttaaag ctgtctgtag ctgagacaag cgctcctcta
    12241 atttttgctt ttcctcttct aaaataccaa gcctctcctc tctcgtttca tctccctttt
    12301 ggtagagcga catatagtca actaaactat ctacagagac acccgccgaa cgaaaacact
    12361 taataaattc cagcgcttcg atatcctgat cttgaaaatc acgaatccca gtagcagtac
    12421 gagtaatcgg tggcacaaga ccaacccgtt cataataccg aatcgtatcc gctgaaattc
    12481 ccaacaaatc actggcagat ttaatattca cacgctaacc tcctctcatt tcattcactg
    12541 aattcacgct attctaactc acttatctta ttatataact ttgagttgac tccaagtcaa
    12601 gcattttgta tctaatcgaa aaatttttat cattaacaat catatatctg gagtttatac
    12661 aatgctcttc tcttaccaat ttttgaaata tttaaataat aaagaaatta ctctattcca
    12721 aataagatag tacccaagta tttttcactt ttttctaatg ttgatgctca taactccgtt
    12781 ccaagtcaca cacctttcgc ttatgggttt ggtgagtttc taggtcagca tcaatttcaa
    12841 tggtaatatt ttgaaaacca caatctttta ggaaaattcg aatagactct ttacaagttt
    12901 ccatatgttc catttctttt agacaaacat ggacaatggc atttttttcc aaagcatcca
    12961 tagtccagag attaagctga ttaaggctgg ccacattgtc caatcgctcc aggccactct
    13021 ttacttgctt gatatcaaga ccttctggca cagcatccaa gaaaatcttg agtgtagacc
    13081 aaaaacgtgg aagggctttt gaaagaataa agaaagaaat gacaagggac aaaagaggat
    13141 ctaggatata ccagtccgta aatcgaagaa caatcgccat caggataaca gctacccacc
    13201 ctagcgtatc ttccagaaaa tgcagactca gaatagactc attctttgtc tttcccttac
    13261 caaccaccag actcgctaac atattgatag taatcgcaat aattcctaac cagagaatcc
    13321 cctcatcatt gactggttgc ggatgcaaaa tcttcgtgac attttccaaa atgactagaa
    13381 cagagcccgt tacgagaatc acagctgtta ccaaggctcc tagcaggcta aaccgcttat
    13441 agcccaaggt gtactgattg tcttcttcac gattggagat tgtttctaaa aaagctgata
    13501 ttccaattgc aatcgcatct accaagtcat gcacagagtc agcaagaaca gcgctagaac
    13561 caaatattcc acctgcaata aactcaacaa tggcataagt caaatttaag aaaaaagcca
    13621 cccaaacagc atattttgcc ttcatatttc tcattccttt gttataatag atttatgaac
    13681 accttgttca ttatcattat ccactaaaac tcaaagaaag gatagaagca aaacgtcagc
    13741 tttattcagt tctgaacaat ttgccttaag tgtccatatg actaacattg accgccgtat
    13801 cagcaaaacc aaaaaagcca tctatcaagc ttttatacaa cttttgaatg ctaagggcta
    13861 cgaggccact actgttcagg atatcattga tctcgcagat gtgggacgat ccacctttta
    13921 ctgtcactat gagagtaagg agcttcttct ggaccagctc tgccgctacc tcttccatca
    13981 tctttttgaa agagagcaag ccatttcaac cgaggactac ctcgcccacc tctttctcca
    14041 ttttcataaa aaccaagacc atatcaccag tctgctattt tccaaaaatg actacttcct
    14101 ccgtcaactc cagagagagc ttgagcacca cgtctattcc gtgctagctg ataatttaaa
    14161 agaagcccac cctaatctgc ctacttctta cctccaacac ttggtcatgt ccaactttat
    14221 cgagacattg acctggtggc tcaaaaaagg tcaagatttc acagaccagg aagttgtcca
    14281 attttatcta gaccttctca ttcctaaaaa ttgaatatag agtaaagctt cagttgtctt
    14341 atttctaggt tactgagttt tttatctttt caacaacaaa agaggacccg ccgatcctct
    14401 ttttcatact ataaatcctt gattatcaac tatatctgtt ttaatcgaaa tctcaaaaca
    14461 gcactttcaa acatcttttc ctagttaagt aaatcagtat tctgcttagc tgccttgctc
    14521 cattgatacc aaccaactag actgttaatg agataaatta gatatttccc ttgaatttgc
    14581 aggctttctc cccaccagag atagattgaa aagacattgg tagccgccca gaatatccac
    14641 tgttcacggt aaacagctgt catgaggatt tgccctaccc cattggttgc atctgtgatt
    14701 gaatcacgat agggacgatt ggcaccaata gactgataaa tgaagccaaa ggccaaccac
    14761 caaagcacac taatggaaag atactttgtc cagcccttgc cgtccagttt acgcgcgaca
    14821 aactcctgct tttccttctt aaactgtgcc tgataaatcc aaactagaag tccaattggc
    14881 tgcatgactg tgaagtaaag tgtcgtcagc acctcaccat aaaagccttt ctgtagggcc
    14941 aaaataaggt aaataacaga gttaatcaag ccaaaaagat aattacttgc tcgaccttcc
    15001 gatacaaaga ttacacagat aatccctgtc aagctacaaa tcatcccaat ccagtcaaca
    15061 atacgatgtt cgtaaaccaa ctccagccag agaggaaaac ttcctaaaac cagcaaataa
    15121 atccactggg caaaactacg atgggcaaag aggtcatccc agatagtctt catagttcct
    15181 gaaaatccta aatcagccat agccgcaacc atacgacggt aaccacctga catttcacct
    15241 agggttgttt tgatattttc aattttcttt tgcaaataag tatgcatcat ttctcctttt
    15301 gtttttaaag agccgtgtct ggatagactt tcggacgcaa cgctctatta gataatgaac
    15361 tgcctataca caagatttct aaccttagtc gacatgagct gaaacccctt atttgttaag
    15421 tagttcacaa aatattatac atctatttta tgaatagtca agtgtcttta cagtaaaatt
    15481 ttagaaaatc atgaaaattt tctctttctt tccattttaa gtgacattca gtcattctca
    15541 catcaaaaaa gcccagacga aattgtctga gcattctttt atctagtcgt ttaaggaagt
    15601 tgagttcagt atgtttaaag tctctgtccc atcatttctt caacaaacct tgttcttgga
    15661 gaaactcctt ggctacttgc tctgctgact tgccttcaac accgacttgg tagttgagct
    15721 ggctcatctg gctttctgta atcttaccag ccaatgtatt aagaactctt tccaactctg
    15781 ggtgtttctt gagaagagct tctttcatga gtggagcccc ttgataaggt gggaagagtt
    15841 gcttgtcatc ttccaagacc tgtaaatcat aacgctccaa ttccgcatca gtcgaatagg
    15901 cgtccgtaat ttgaatatca cctgactgaa tagcctgata gcgaagggct ggctccatgg
    15961 tcgctacatt gagattgaga ccatacattg attgcaagcc cttatttcca tcttcacggt
    16021 cgttaaactc gagtgtaaaa cctgccttca actgcccttc cacttttttc aagtctgaaa
    16081 tggtcttcaa gccatattct tgagcaatct ttttcggaac agctacagca taggtgtttt
    16141 gataagacat gggtttgaga taggctagat gatcctgctt agcaatgcca tcacgcgcca
    16201 cctgataaac ctgttctggt tcatgactca ccttgggtga tggttgaagc aaactttcag
    16261 tcaccgtacc agtaaattca ggatagatgt caatatcgcc ttttttcaga gcttcataaa
    16321 ggaagcttgt cttcccaaaa ttcggtttaa cagtcgcagt catgctggta ttttcttcaa
    16381 tcagcaactt atacatattg gccaaaattt ctggttctgg acctattttc ccagcaataa
    16441 ccaagttttc cttctctttt tgaaccaaaa gagctggact ataagacaga cccagtaata
    16501 aagccaccaa ggcaaaacct gagaaaatcg tccgtaattt tgctttttcc attactttta
    16561 gtaggaagtt aaaggcaata gcaagcactg cagaagaaag tgccccaatc aaaatcaaac
    16621 tggcattatt acggtcaatt cccaacagga taaaggaacc cagtccccct gctccaatca
    16681 aggccgccaa ggttgccgta ccgataatca aaacagctgc cgtccgaatc ccagacatga
    16741 taacaggcat ggcgagtgga atttcaaatt tcttgagacg ttcccatctg gtcatcccaa
    16801 aggcaatccc agcctcttgc aggttcggat caattccctt cagcccagtg atagtatttt
    16861 gcaaaatagg gaaaatcgca taaatcacta gagctgtcaa agccggcaag gtcccaattc
    16921 ccatcaaagg gataaagagc cccaacaagg ccagagacgg gatggtctgg aaaatacctg
    16981 caatctgcaa gacccagtcg gccagcttct catgatagcg aagaaaaaca gccaagggaa
    17041 tcgcaagcaa aatagctagt aacaaggtca aaagtgacaa ctgcaaatgt tgagatagag
    17101 ctgtcaacca atcactaaaa cgatcctgaa aagttgcaat taaattagtc atgaacacta
    17161 cctccaaaca agtctgctac aaagtctgtt gcaggcactt ttaaaattgt ctcgggattc
    17221 gctacctggc gaatttctcc atcctgcaag acagcaatac ggtccgccaa cttcaaggct
    17281 tcatccgtat catgggttac aaaaatcgtt gtcatcccaa actctttatg caattctttt
    17341 gtcagaacct gcaactgttt tctcgaaata gcatccaagg ccgaaaaggg ttcatccatg
    17401 aggaaaatct tgggctgacc aatcatagct cggacaatac cgacccgttg ctgttctcca
    17461 ccagataatt cactaggtaa gcgatgccca tactcggcta ctggtaaacc aaccttagcc
    17521 aaaagctctt ctgttttctt cgtaatttct tccttgctcc accccttcat ttcaggaatg
    17581 agagcaatat tttccgcaac tgttagattt ggaaaaagag caatagcctg taaaacataa
    17641 ccagtagaaa gacgaagttc acgctcatca tagtctttga tgcgcttccc atccatataa
    17701 atatttccat cagttggttc caaaagacgg ttaatcatct tgagcatggt cgtcttacct
    17761 gacccagaag gccctactaa aaccataaat tccccatcct caatctgtaa gttgacatct
    17821 ctcaagacat ccttttctgt gtagcgcagt gctacatttt tgtattcaat cattctttgt
    17881 cctcaattta aaacttccct cgattagtca agtcttctac cttaggcatg acttccttat
    17941 tatcccaatg ctccacaatt ttcccgttct ctaaacggaa gatatcgtac tgggcataag
    18001 caacgccatc aatctgagtc tgaccatagc taaccacata gtttccttgt cctaagagtt
    18061 ggaaaacaaa gtcaaaagtg acactatatt cagccacata gtttttataa gcagcacttc
    18121 cttgtccaat ctcatgatta tgctgaatca aatcgtctgc cacataatca ctccactgct
    18181 ctagctcccc attttggaaa atttctgtca agaaacggcg aaccagcttt ttattttctg
    18241 ctttcttatc caaatccttg atttcaaaat ctccaaaaat ttgatctagt tggtcatttt
    18301 caggtgttcg atagtagtca atgacatccc aatgctcaac aatacaacca ttctcatccg
    18361 cacggaaagt atccgtcgtc acccattgag cttctccacc attcagatat tgatgaacat
    18421 gaacaaagac cagattgcca tcctcaatgg tgcggacaat cttaatctga cgctctggat
    18481 gacgctcaaa gaaatctgca aagaaggctg caaatccttc tttcccgtca ggaacacctg
    18541 tcgaatgttg gatataggta tcccctacag actgggcttg agcctcagca actcgtccgt
    18601 cttgaatggc atggatgtat aggttgtgag catttttcac ttgttgtgac atattctaaa
    18661 cctcatttcc cttctctttc agattcgcca aaattctttc ttgaaaacct tcaaattggt
    18721 gaatttcttc ctctgaaaat cctttgtaaa agatagtatc caatttctga ctgacacgat
    18781 gccccacttc tttctgggac ttgcctaact ccgttaaaac taaatacttc ttacgcttgt
    18841 cttttccaca cggactaaca attacaagct tttgttcctc tagctttttt atcatagtcg
    18901 tcagcgtatt attcgcaagc ccagtcgcaa gcgcgatatc tgtcgcagtt gcgcagccag
    18961 tttcactatt ccataaaacc gctaaaatct tgccctgttc acccctataa agagcctcag
    19021 gatcttgact cagtaacttt tgaaaaatcc gcccattcaa caaacgaata tgatgggcta
    19081 gcaaatgact atctttcata acacctccaa tttatttcga tgtcgaaatg aataaaataa
    19141 ttgtaacact catcgttcta actgtcaact atttcgattt ggaaataatt tttgataatt
    19201 attcacacca ccatactccg gctcaactaa cttttaacga gagtttctaa attccttcgt
    19261 cctccagtct acaaaagcct tccatccgta ctatcctatt ttttatgagg ggacacattt
    19321 ttcctatcag atcatttatt ttaaagatag aagtaaatca taattgcttc catctgttct
    19381 tttatagtat attgaagttg gactagggca ctgtatcttc taaaacattg atagaaagcg
    19441 atttgaattt cccaatcaat ttgttcgtat ttatagcatt ttgaaactag aataggacgc
    19501 catgactgct aaaagatttc tataaattca tttgattttc ctaatcaatt tgttcatatc
    19561 ttatttcatt ccgctataat ttcaccttac cctatctttt tcgtagcacc cttcaaacag
    19621 cctatcccct accgtttgac gattcctcac ttcactccac ttccattaca gaagtttctt
    19681 cactactatg gtctcggctg acttctcatg attccttgtt actactattt gaacgctcac
    19741 gagataggtc ttataaaaaa tgctttgatc cacaatggaa tcaaagcatt ttaaagagtt
    19801 cctcatacat aagcgcagaa gtcgcagttc ctctgtactt ggcttcttct cttttgataa
    19861 agcgagccaa gttgagcaac tcaggtgctg gatgtttggg atttaggagc aattcacgat
    19921 tgaccaggcc tgagagacga actgcctgca attgctcatt tgtagtaggc agttttttag
    19981 tagtctctag gagagcagca actaaatctt cactcaaatc atgtcgagca tgattgtaaa
    20041 gatcttttat aaggctttct aggtttggtt ctgccatccc taccacctcc cttatggttt
    20101 aataatgttt aatcaaatca accgttgaac gatccaattt cttcaccaag gcttgtaaga
    20161 aagcttgcgc ttctaggaag tcatccattg catagagggt ttggtgagaa tggatataac
    20221 gagcgcagac accgatagtt gttgatggga caccaccatt tttcagatga gctgcacctg
    20281 catctgttcc gcctttacca cagtagtatt ggtacttgat accagcttct tcagccgttg
    20341 tcaaaaggaa atccttcatc cctgggagaa gcaagtgacc tggatcatag aaacgaatca
    20401 aggttccatc tccaatcttg ccttgaccac cgtagacatc acctgctggt gagcaatcaa
    20461 ctgcgaggaa gacttctggg tcaaacttgg ttgtagaggt atgagcgcca cgcagaccaa
    20521 cttcttcttg gacgttagaa cccagataga gttcattgcc gagtttttga cctgataaag
    20581 cttcagctag ctcgcttacc atgaggacac cgtagcggtt atcccaagct tttgagatga
    20641 tatttttttc attggctgtc aaaattgcag aactatctgg tacgatggta tcaccaggac
    20701 ggatgccaaa actttctgcc tcagccttgt ccgcaaaacc accatcaaaa acgatatcgg
    20761 caatggctgg catggttggt cccccctttc cacgagtcaa atgcggagga acagaacctg
    20821 aaatcacagg aatttcatga ccatcacgag tcaagagttt gaaacgttgg ctgctaacca
    20881 ccatggggtt ccagccaccg atttctacga cacggaaggt accatctagc ttgatttcgc
    20941 tgaccataaa accaacttcg tccatatgag aagcgaccaa gacgcgcggt gcatccacag
    21001 cttctgaatg tttgatacca aaaataccac ccaagccatc tgtcaccact tcatccacat
    21061 gcggtgtcaa cttttcacga agataagcac ggacaggcgc ttcatgacct gagactgcag
    21121 caagttctgt tacttcttta atttttgaaa ataatgttgt catttcagtt ccttctttct
    21181 ttcatccatt ttaccacttt ttataggaga aggatagtgg gaaggtggat ttctaagtta
    21241 gtatcttagt cctgctctat cttagaaaag gatagtattc tcttgcatgt agtgcaaaat
    21301 ctagtaaaca ttccaaaatt aactcgaata tttatttcca aacaaaaaaa caatacacca
    21361 tcaaagttgt ttagattttt catgaaattt acagaaaata gttgacttcc ctttcttctt
    21421 tctttaaata tatagttggt tgagtttgga atagtacgct gtagctgcta aaacatttct
    21481 agaaattaat ttgactttcc taatagagtt gttcatatct tatttcaatt tactatagta
    21541 caaaactaga aaaggaaaaa atcatgacca aaacaattct tgttacaggt gggactagct
    21601 acattggctc ccataccgtt aaagctcttt taaatgctgg ctatcaggtg catgtcctcg
    21661 ataatctctc cacaggaaat cgagcggctg tggatagtcg tgctagcttt aaagaactgg
    21721 atgtttatga tgctagcgcc ttaaaggctt acttagaaga aaatcagatt gatgctgtcc
    21781 tccattgtgc aggtgaaatt gttgtgagcg aaagtattga aaatccaagt aaatacttca
    21841 ctgccaacgt tgctggtatg aaccaagttc tcaaagtctt atctgaagtt ggcattcaaa
    21901 aaatcatgtt ctcttcgact gcttctctct atggaaataa ctgtattgac aagccggtga
    21961 ctgaagatac cctgctcgac cctgtcaacc cttatgcaga gacaaaactg atgggcgaac
    22021 gaatgattta ctggatggcc aatcgctacg actggaaata tgttattttc cgttacttta
    22081 atgcttctgg ggctgaaatg gatgcttcaa acggtctgcg tgtgaaaaat ccaactcaca
    22141 tcattccaaa tatcaacaag accgcattgg gacaaaatga tagcttaaaa atatttggag
    22201 atgactacga tacacgtgac ggttcatgta ttcgagatta catttatgtc ttggaccttg
    22261 cataggctca tgttaaagga atgaactacc tatttcaaga agacagttct tctcaaatct
    22321 ttaacttagg aactaaaaaa ggctatacta tcaaagaaat ctttaaaact gctgaagaat
    22381 tactaaatta aaaaatccca cacgagattg ttgctcgacg tgctagtgac ccagccagtg
    22441 tcctagcaga tgcttcaaaa gctaaacaat atcttgattg gacggctagc tactccctcg
    22501 aagatattat tttatcagat taaggtaaaa acattttaga ataggataac aaaggagaaa
    22561 gtcttgttgc tctctccttg ttatatttat taaattgtca gaaattttat tgacagatta
    22621 gaaaaataat gttacattta tatccgcaga tatctttcga taccaaatct acatgaaagg
    22681 acggggtatg aaactttctc attatttaat tggcttactt ctactcctag tctttctctc
    22741 tattagcatt gggaccagtg atttttcatg gggaaagcta tttgatttcg accagcagac
    22801 ctggctcctt tttcaagagt cccgtctccc aagaactatc agtattctcc tgactgcctc
    22861 tagtatgagt atggcaggcc ttctcatgca gactattacc caaaatcagt ttgctgcacc
    22921 gagtacagtt ggaacgactg aagccgccaa actgggaatg gtgctgagcc tttttgtctt
    22981 tccatcggct agtctgaccc aaaagatgct cttcgctttt gtttcatcca tcgtattcac
    23041 cctcttcttc ctagccttta tgaccatttt tactgtaaag gaaaggtggg tgttgcctct
    23101 gattgggatc atctatagcg ggattatcgg ctcagtcaca gaagttatcg cctatcgttt
    23161 caatctggtt cagagtatga ctgcctggac ccagggctcc ttctccatga ttcagaccca
    23221 tcagtatgag tggctcttct taggcctcat catcctgata accgtttgga aattatccca
    23281 aaccttcacc atcatgaatc tagggaaaga aaccagcgag agtttgggga tttcctactc
    23341 cctacttgaa aaactggccc tctttctggt ggcgctaacg acaagcgtca ccatgattac
    23401 cgtgggtggc ctaccatttc tcggagttat cgttcccaat cttgttcgca agcgctatgg
    23461 agataatcta agtcaaacca aactcatggt cgcactggtt ggtgccaatc tagttctggc
    23521 ttgcgatatc ctatcccgag ttctgattag gccctatgag ttgtctgtca gtctcttgct
    23581 aggaatcatc ggtagtctcg tctttatcct acttctctgg agagggggac gaaaagatgc
    23641 agactaaaag caaacatacc aagctcttct ggattctcat tattcttgcc atcggagctt
    23701 gtcttctcta cttttggccc atcactcact tgtcagcctt tgcttggaag ttgcgttccc
    23761 aaaagatcat cgtttatctc ttggtagcca tcgcgactgg gatttcgacc attagttttc
    23821 aaaccctgac ggaaaatcgc ttcctgacgc ctagtatttt gggaattgaa tccttctacg
    23881 tcctactaca aaccctacta ctggtttttg aaagcaagtt tcttcaactt ggcaaatccc
    23941 ctatcttaga attcctagtc ttacttcttg tccagtccct cttctttctc gccttacaag
    24001 gttacttgaa gacactgatg aagcaagacc tggtcttcat cctgctgaac tgtctagcgc
    24061 tcagaagtct ctttcgaaat atcagcacct tccttcaagt cctaatggat ccaaacgaat
    24121 acgataaact gcaaaatagt ctttttgcct cctttcaaca tctcaacact tccatcctag
    24181 ccatcggttc tctgatcatc ctcgctttga caatcttttt ctttcgaaaa gcagtcgttc
    24241 tagatgtctt gcacctgcaa agagaaacgg ctcagatatt gggactcgat gttgaaaaag
    24301 aacagaaaga gctcctctgg ggaatcgtgc ttttgacctc aacggccact gccttggtag
    24361 gacctatggc cttcttcggc tttatgctgg ccaacctcac ctacctgatt gtcaaagact
    24421 atcagcacaa gttactcttt atagtggcca ttctggttgg atttattagc ttaaccttgg
    24481 ggcaagcctt gattgaacga gtctttgcac tggaaattcg tatcagtatg atcattgaga
    24541 gtgtgggtgg cttcttattc tttatcttac tatataggag gtctcgtcag tgaaactgga
    24601 aaacattgac aaatccattc aaaaacagga tattttgcaa ggcatttcgc ttgaagtcag
    24661 tcctcaaaaa ctgacagcct ttattggtcc aaatggtgct ggaaaatcga ctctcctctc
    24721 catcatgagc agactaacca agaaagatca gggagttctc agtatcaaag gacgtgaaat
    24781 cgagagctgg aattcgcaag aactggccca agaactaacc atcctaaaac agaaaatcaa
    24841 ttaccaagcc aaattgactg ttgaagaact ggtcagtttt ggacgttttc cctacagccg
    24901 aggtcgactt agatcagaag actgggaaaa aatccgagaa actctgaact atttggaact
    24961 gaccaactta aaagaccgct acatcaatag cctgtcaggg gggcaactcc agcgcgtctt
    25021 tatcgctatg gtactggccc aggatacgga ctttatcttg ctggacgaac cactcaacaa
    25081 tctcgatatc aagcaaagcg tcagcatgat gcagattctt cgacgactgg tggaggaact
    25141 cggcaagacc attatcatcg tcctccacga tatcaacatg gccagtcagt atgcagatga
    25201 aattgtcgcc ttcaaggacg gccaggtctt tagcaaggga agaaccgatc aaatcatgca
    25261 ggctgacctg ctcagtcaac tttatgagat tcccatcacg ctagctgata tcaatgacaa
    25321 aaagatctgt atctatagct agtaacataa aagctcaagt tagagaacct tcagtctctt
    25381 agtcaataag attaagagac tccctaaatc gttatcacat tttaaaaagg agaaattatg
    25441 aaaacatccc ttaaacttta tttcattgcc ctagtggcca gtttcttgct cctacttggt
    25501 gcatgtagta caaactcaag cactagtcag acagagccca gtagctctgc tccaacagag
    25561 gtaaccatta aaagttcact ggacgaggtc aaactttcca aagttcctga aaagattgtg
    25621 acctttgacc tcggcgctgc ggatactatt cgcgctttag gatttgaaaa aaatatcgtc
    25681 ggaatgccta caaaaactgt tccgacttat ctaaaagacc tagtgggaac tgtcaaaaat
    25741 gttggttcta tgaaagaacc tgatttagaa gctatcgccg cccttgaacc tgatttgatt
    25801 atcgcttcac cacgtacaca aaaattcgta gacaaattca aagaaatcgc cccaaccgtt
    25861 ctcttccaag caagcaagga cgactactgg acttctacca aggctaatat cgaatcctta
    25921 gcaagtgcct tcggcgaaac tggtacacaa aaagccaagg aagaattggc caagctagac
    25981 aagagcatcc aagaagtcgc tactaaaaat gaaagctctg acaaaaaagc ccttgcgatc
    26041 ctccttaatg aaggaaaaat ggcagccttt ggtgccaaat ctcgtttctc tttcttgtac
    26101 caaaccttga aattcaaacc aactgataca aaatttgaag actcacgcca cggacaagaa
    26161 gtcagctttg aaagtgtcaa agaaatcaac cctgacatcc tctttgtcat caaccgtacc
    26221 cttgccatcg gtggggacaa ctctagcaac gacggtgtcc tagaaaatgc ccttatcgct
    26281 gaaacacctg ctgctaaaaa tggtaagatt atccaactaa caccagacct ctggtatcta
    26341 agcggaggcg gacttgaatc aacaaaactc atgattgaag acatacaaaa agctttgaaa
    26401 taagccactt caaactcaat ttaatactca atgaaaatca aagagcaaac tagaaaacta
    26461 gccgcaggtt gctcaaaaca ctgttttgag gttgcagatg aaagctgacg cggtttgaag
    26521 agattttcga agagtataaa attttgacat tctaaaacgc atcctatcaa gtttattcaa
    26581 acctgatagg atgcgttttt atcattttga aatctattta ccccacctca ttccccttct
    26641 tgatttcgtt taagggaaaa gtggtctgga acggggtcct tacctgtttt cgaccaggga
    26701 tgacaacgta aaatccgagc caagctcatc aataccccct taaaaccatg tttttcaata
    26761 gcctgaatca tgtagttgga acaagtcagc tcaaagcgac aagagggtgg aaagactggt
    26821 gagataaaac gttggtaaaa gcgcacaggc gctattaaaa ttcgtttcat tatttcttag
    26881 ttacagccat ggtgtgtagt tggctgattt cttttttatt aagacgacgg gattctcctg
    26941 gacggagtcc tgtcaagtct aggtgtccga aacgagtccg agacaactta tctacttgga
    27001 gaccaacagc ttcaaacatc tttttaacct gatggttacg cccttcatgg atggtcaact
    27061 gcaccacaga gcgatttttg actgggtcca ctttgagaat ttcataaaca gctggcttgg
    27121 ttttcttacc atcaatctca agaccacggg tcaaggggcg aagattgtcc ttattggcca
    27181 cacctttaac acgcgcgaca taaaccttgt caatctcatt acgagggtga atcatctcgt
    27241 ctgtaaagtc cccatcattg gtcaaaatca agactcctga tgtatcccag tccaaacgac
    27301 ccacagggta aatgcgctct ttgacattgg gcaagaggtc gacaaccgtc ttgcgaccct
    27361 tgtcatctgt tacactggaa atgacaccgc gtggtttgtt aagcagataa tagacctttt
    27421 cttcgttgta gataggttga ccttcaactt cgaccttgtc gcctgacttg atagtggttg
    27481 ctagttcacg caccacttgg ccgttaaccg tcaccaaacc ttgcttgatc agctcttctg
    27541 cttttctcct actggccaca cctgcgtggg caatatactt attgattctc atcttcttct
    27601 atcctttcac caaataattg gctttcttgg gcttgaatct caagctcatc aatcactggt
    27661 aattcttcta aatggtttat ccccatgtaa tctaggaaat aatccgtagt cacatagagg
    27721 ttggggcgcc ccaatacttc ctttttcccg tcttccttta tcaggtcaaa agcttgcaac
    27781 tttgccaagg ctccactcga gttaacccca cggatggcat caatttctat ccgcgtaatc
    27841 ggctgtttgt aggcaataat ggacaaggtc tcaagggcag cccgagacaa gctctggttg
    27901 ataggcgcct tagagtattc cttcaaaatc tctgcaaatt gaggcttggt caccaatcta
    27961 taagcaccac ttgtctcaat caaagccaaa ctggaatctg ggtccttttc atacttctgg
    28021 gctaattttc ctaaactctg ctggatgcct gtcggtggca gagagaggag ttcagctaac
    28081 tggcggaccc gaatcccatc ttcacccgct acaaacaaga gcgcttctat ttttgctaaa
    28141 gtactcatct ttcctctcta tcaagtctag ctttgaggca cttgactttc ttccttcttt
    28201 tccatgagat agatatctcc gaaactctcc tcttgcacga ggatcagctc ctgggttttg
    28261 attaactcta gggttgccaa aaagagggtg atgacctctt ggacattctg ggcttccttg
    28321 aacaaatcct gcaagcgcaa ttgatctcgt ccaatcaagg actctttcac gataatcatc
    28381 atgtcctcaa tcttatactc atcccgcaag atagtcgtgt gattttgtgc aaactcctct
    28441 tttttcttgg ctaggatatt tgaaaaagcc aaaaagaggt caatggtcgt cttgtcatgc
    28501 acaagctccg catcttcgta aatcaactct gtcggcgctt tggaataata ctgggcccgt
    28561 tcttggtgct tggcttccaa gtgctcaccc aagagcttga acttgcgata ttcttcgatt
    28621 tgagagagga ggtcctgctc caggtcatcc cccaagtctg tcacttctgc taccttcgga
    28681 aggagtttac gactcttaat cagcatgagc tgactagcca tgaccatgta ctcacccgtc
    28741 acttccagac gcatggcctg cagggttgag acataggcta gatactgttc gatgacttcc
    28801 gtaatgggca catcgtagat atccatctgg tacttagaaa ccagatgcaa gagcaagtcc
    28861 aggggtcctt caaaatcttt taatttaata tccattatct atatttttct aaggtcagga
    28921 ctgtttttaa tcctaatttt tttgcaattt cgtacaaatc gaccttgttt tctatttgtc
    28981 ttaaaataaa ctgttcacgt aagacttgag ctgataagga tggaaaacct ttctccttga
    29041 caaaagattc taactgacga aaggcccact gacgagaata gggtttccct cctctttcaa
    29101 aaagataggt ctgccccatc aagggttcca attctgaaag caaggccgtg ggaatggtga
    29161 caatcctctg ttgggaagcc ttgctgattc gcaacacctg aaaatccaga ttgatgtccg
    29221 caaccttgat ggctaaaatc tcactgggca agagccccat ttctaggatt aagagcgcta
    29281 gcaagcggcc ctctggatgg tcgctttcct gccaaaaaga gtctaggtat agaatctctg
    29341 gcttttccgt cttcttttca gcttgtttgg ctaattccaa gcggtaaaag ctgtccacct
    29401 ctcctttttg atagagaaag tagagaaatt ggttacaggc cgaaatcttt cgcttctggg
    29461 cgctgatttt tagattggct agctgggctt ggtaaatctt gagactggtc tcagaaatcc
    29521 gctcacctac catgtctaaa aattgctcca aatcatactt ataggactgc ttggaattga
    29581 cagacaagcc ctgcttttcc tctaaaaagg ctgaaatcct gtctctcatt ggcatcgaat
    29641 ctcatattcc ttactaaaat catgcaagag ggcattaacg gccttgagga tggacttgcg
    29701 cgtaataatc ccttggaaaa taccctctgt atccacaacc ggtaagaagg actcatctac
    29761 tagcttgtgc aagacctccg taatggtgaa atcaggcgaa acaaccgcta cgtccgtttt
    29821 tgtcatatga acgatatccg tatccgccat gatttcttgg ctcaagtcat gctccatctg
    29881 ataagccata atatctctga gtccaatcgt cccaacaaac tgtttttcat ctgtcacaac
    29941 gggaacacgg gtataggtca tctgactgag caagagggtc gcatgatccg cattgtgggt
    30001 atcaatcaac acagctagat ttttagcagg ggtcaaaaaa gtttcctcct gccccaacaa
    30061 gaaagtctca aactccttgg caatcatcgg ctaaactcct tggacaaacc tggatacacc
    30121 tcgtgatctc gtgtcaaaaa gtccactttg aagtaactat catcaatctc cacacgagca
    30181 tagagacatt ctctgatggt acctcgtggt tgactgatag aacctggatt tagaaagagg
    30241 atcttgcctt ccaaccaagc acttggcaca tgcaagtgac catagaggca gatagcggcc
    30301 tcttcctcct gagcccagta gtccaacttt tgaaagttga aattgatgtc aaacaagtga
    30361 ccatgagttt ggataatctt ggtcgaacca agctcagtca ccagacgttc tgggtagccg
    30421 gcgtagaagt ccatgttccc tttaacaacg cggatgccct cccaaagtgg agaatccgga
    30481 cgtagttcag aatcgccgtt atgaaaaacg gcatcgactt tgcccacata gcgatcacgg
    30541 acttcttcca caatcaagct atcgccatgg gaatcgctca ttacaatgat ggtttgcttt
    30601 gccatgatgg aaatacctcc aaaagtttct taacggctaa ggcacggtga gattgactat
    30661 ttttttcttc cagggttaat tcagctgatg actcacctgt ctctcctaca aggaagaggg
    30721 gatcatagcc aaagccattt tcacccttag gttcaaagtt aatataacct gaccagtctg
    30781 cttcaacaac taaactttcc ttatttgggc tggctacgac tagggttgtg tggaactgag
    30841 ccgagcggtc cttgagttca aagaccatgg ccaattcgtg caagagtttg gcattatttt
    30901 cacggtcagt tgctcccaca cctgcgaaac gagctgacca gacgcctggt aagccaccaa
    30961 ggacatcgac tttgagacca gaatcatctg ccaaaaccat cttgcccgtt aattgagaaa
    31021 tggtttctgc cttaaggcgg gcattttctt caaaggtcat acctgtttct gctacttcag
    31081 gcaggtcagg gtagtcatta agattttcca catcgtagcc taacttatca aagatagctc
    31141 ggaattcctt ggttttacct tcgttacgag tcgcaatcaa caaggtttct ctgaccttgc
    31201 ttgtctcaaa gaaatcatga acattgaccc cttctttagg caattctaca tacaagagtt
    31261 gcccattttc aaccaagagc agggccccat gacgttgaat aacttccaag tttcgtccca
    31321 tttcttgatt aagcatgtct accacaaagg agaacaaacg gtaggtagaa ccatagcgta
    31381 aaacagtaac tgaaagcggg aaaccatact cttcatctcc aaatattttg gagaattcaa
    31441 acagaggaaa atctgtctta tagtcgctca aactgttaac gccaccaaaa atactataag
    31501 acccaacata ccagtcctgg tcatccttat attcataaat tttatttgtc ataattctac
    31561 atgctccaca tgaatctctt tttccagcca ttcttcacca atttgtgcaa aactttggct
    31621 actggctgtt gtgtaaaaac ggtgatggag tggtccagca tcgcgaccac gattgatttc
    31681 aaaataattg agtaagactg agatatcccg tacgcactct gccccactat cgatgagctg
    31741 aacctttggc cccatcacat tttggataat agggcgaagg agtggataat gagtacagcc
    31801 caaaatcagg ctatccacct ttccaaccaa gggacgcagg gtttcataga ccaccttctt
    31861 ggtaacactg gttgacaggg cacctgactc aaccaaggga gcaaacttgg gacaggccaa
    31921 gctctccacc tgtaagtcgg gatccagatc atggattttc tgacggtata tgtctgattg
    31981 taccgtcatg ggcgttccaa tcactccgat tttcccacct tgactggact tgatggctgc
    32041 cgaagctcct ggcaaaatta cacccaagac aggaatatct agttgagcct tgatttcttc
    32101 ccagacgacc gcagtcgcag tgttacaagc aatgacaatc attttgacat ccttggtcaa
    32161 gagaaagttg accagctgcc aagtatattc acgaatttgc tcagcaggac gggggccata
    32221 gggcgcccgc gccgaatctc caatatagac gatttcttca tggggaagct ggcgcatgag
    32281 ctcgcgcaca acggtcaagc ccccgacacc cgaatccaaa aaaccaattg gtcgattatc
    32341 catacagtct tctttctagt cttttttctg attgatcgtt cattatgatt ccttgtactg
    32401 aacgaactga ccgacagctt gatagaatgt caactacctc tctcttaatc ataatcaaat
    32461 atcaatagaa tgcaaggaaa aagtctcatc cctaaaccat aaccaacata gtgagaagtc
    32521 tggaactttc atcccagact tttccttatt tattttttct ttttattgtt agcaagggct
    32581 gccttttgtt ggcggatgat ttggtggtaa acttgttgta ccttagcttc gcttggtttt
    32641 tgaccatttg cactcaaaag agtacgaact gcttcagcat tcaaacgtgg gttgtcagca
    32701 aattcttttt cgatttgctt acgaaccaag tacattcctc caagagctcc tcctagaaaa
    32761 gctagcacaa tcaatacaat tgctaaaagt aaatccatca tttttctcct gtttcttttt
    32821 gaatcccttt cattatacca caaactagcc accctgacca gtttgttcta cactttcaag
    32881 gatggaatag acagcaaaaa gaaccctgct tttctgaaaa agaggtctcc ttgctaagtt
    32941 tatactcaat gaaaatcaaa gagcaaacta ggaagctagc cacaggttgc tcaaagcacc
    33001 gctttgaggt tacagataaa actgacacgg tttgaagaga ttttcgaaga gtattaattt
    33061 acataaatag ccagtgtttg atagggtttg agtagaattt tctcagacac ttctgcatct
    33121 tcatagtttg aaatcaaaat ctgtccattt tggtagactg ctggcaagtc gatttccact
    33181 tctttggcat aaaagttatt gagcactagt aacttttgat cctcaaactg gcgttcaaaa
    33241 gcgtagactt gtttgctatc ttcaaaggct ggtttgtaat ttccttctga aatgattggc
    33301 atttccttac gcagtcgaat caagtcttga tagaaggtga aaatcagacc tttgatttca
    33361 ttttctacat tgatatcttt ataggattta ccgactttca accatggagt gcctgttgaa
    33421 aatcctgcat tttcagaagt atcccactgc atgggaatac gtgaattatc acgcgactta
    33481 gcttgaataa tctggaaggc ctcttgctga ctctttcctt cttctaagag catctgatag
    33541 gcattaagcg attcgacatc cacataatca gccatagaat catagtctgg gtcaatcatc
    33601 ccgatttcct cacccatgta gatataaggt gtcccacgtg acaggtgaat gctggctgct
    33661 agcatggtgg ctccttcctt gcggaagttt tgaatatcga caaaacggtt caaggcacgt
    33721 ggttgatcgt gattgttcca aaagagggca ctccaaccgt ctttatcact catttcctta
    33781 ccccaactat ggtaaagact cttcaactct tcaaaatcaa agggagccaa ggtccacttt
    33841 tgtccatcct tatagtccac cttgaggtga tgaaaattaa aggtcatgga taattcctga
    33901 cgatcaggcg acgaataaag gacacagttt tccatggtgg tagaagacat ttccccaact
    33961 gtcataaagc tatcgtcaga tccaaaagtg gcttggttca tcatacgcaa atagttatga
    34021 acgatgggtt tgtctgtata agctggcttc ccttcatttt caggacagtc cactgaaacc
    34081 tcgtccttac cgatcaaatt gatcacatca aatcgaaaac ctttgacacc cttgtcgcgc
    34141 cagaaattaa caaccttgaa aagctcctta cggacattgg gattgcgcca gttaaggtca
    34201 gcctgggtct catcaaatag gtgaagatag tatttcccag tatccccgaa aggcgcccat
    34261 gcagaaccac caaacttaga ctgccaatct gttggttggt cttggatgaa gaaaaagtct
    34321 tgataatact tatcaccagc tagggctttc tgaaaccatt catgctctgt cgaacaatga
    34381 ttaagtacca tgtccagcat aaagtcaatc ttgtgctctt taccgacaca caccatttcc
    34441 tcaaaatcag ccatatcacc aaaaagagga tccactgcca tataatctga aatatcgtaa
    34501 ccattatccc gttgagggct tggatagaat ggattgagcc agaccatatc cacacctagt
    34561 ttggctaaat agggaatttt ttcgataatc ccacggaaat ccccaatacc gttttcagtg
    34621 gtgtctttgt aagattttgg atagatttga tagactactt ttcctttatc aagtgtcatc
    34681 tgtttctcct tttctgataa aagggaggaa gcagtcttcc gtccctattt gtgctatttc
    34741 aattatactc aatgaaaatc aaagaacaaa ctaggaagct agccacaggt tgctcaaaac
    34801 actattttga ggttgcagat agagctgacg tggtttgaag agattttcga agagtattag
    34861 attcgtgtag cgaccatgag agatgctcca gcttggatcg ttgtcggata agttccagta
    34921 atagtcgctg tataagcatc ttggttggtg atgataacag gagtttctgt caccagacct
    34981 gcagccttaa tgacatccat atcaaaacga atcagttgct ggccaactat aacgtgatct
    35041 ccttggacta caagactttc aaaacctttg ccatcaagac ctactgtatc cataccgatg
    35101 tggatgagca attcaactcc ctcgtcagag acaatgccga tggcatgctt ggtagggaaa
    35161 agaaccgtca ctgttccatt aactggagag gtcaactcac cttggcttgg ttcaatgacc
    35221 agaccttgcc ccatgacacc tgatgcaaaa acaggatccg tcgcttgact caattctttc
    35281 acttggccag ttagtgggct gataatttct accgaagtaa gttctactgg ttcatggctc
    35341 acaaattctg cttcttcttg agcaacgaat tctgcctgca agttcgtatc gccctctatt
    35401 tttgtgaaga gaccagcctt gcgaaagaag aaagtcaaga gcattggaac aacaatcgca
    35461 actagcatag ttcctgcaaa tggcagcatg tattgaggtt gaatagagag aatacctggc
    35521 aaaccaccga taccaataga agccgcagtt acattaaaag taacggataa catgcctgca
    35581 agggctgaac cagtcatccc agcaacaaat ggataaatat attttacgtt aaccccaaaa
    35641 agagctggtt ctgtaacacc gagataggct gaaatggttg caggaagtga aacctgagcc
    35701 tcacgctcat catggcgatg catgaaataa taggcaaaca cggctgagcc ttgagcaata
    35761 ttagaaagag caatcattgg ccatagggca gtgccaccag catccgcaat caattgtgta
    35821 tcaatggcat tggtcatatg gtgcagacct gtgatgacaa atggagcgta gagggcacca
    35881 aaaattgcac cgaagagcca tttaactgga ccagttaaac ctgccaagac aactgatgaa
    35941 agtccttgtc caattgtcca accgattggt cccaaaacag tatgagccaa aatcaaggct
    36001 ggaatcaatg acaagaaagg tacaaaaatc atagaaatga cttctgggat atgcttgcgc
    36061 cagaagattt caagataaga cagactcaaa cctgcaagca aggctgggat aacttgggct
    36121 tggtaaccga tacgattaat agtaaaatag ccaaaattcc aaacccagtt tgccgcgata
    36181 tcagctgctg gcgttgaagc aaccgcatag gcattgagca actgaggcga taccaaacag
    36241 attccgagaa caattcccaa aatttggctg gttcccatct tacgagaaac agaccaagta
    36301 atccctactg gtaagaactg gaagatagct tcaccaggca accagaggaa gtgattaacc
    36361 cctgcccaaa actgagagga ttctgtgatg gtcttgccat ccaacatcga ccaatggaca
    36421 ccttccaaga cattacggaa accgaggatc aatccaccaa caatcaaggc tggaataatc
    36481 ggagtaaaga tttccgccag agtggtcata acacgttgga ccacgttttg attactctta
    36541 gctgcagact tggctgcttc tttggaaaca ccctcaatac ctgaaacggc tgtaaaatca
    36601 ttataaaaga tgggcacgtc atttccaatg attacctgaa attgacctgc atttgtaaag
    36661 gttcctttaa cagctggaat tgactcgata gctttaacat tagccttctt atcatctcct
    36721 aaaacaaacc gcatccgtgt cgcacagtga gttacggcag tcacattttc tttgcctccg
    36781 attgcctgaa gcagatcttt ggcttcttgt tcaaattttc ccatttgatt ctccttatac
    36841 ttgtcaaagc aagtttgatt tgataagtat attgtaaacg ataccaaact tgtctgcaac
    36901 ttgtttggta taaaaagggt gtttttttgt aaattttcta gcacttgtat ggtaaaatag
    36961 atacaggtgt tcattaaact agactaaaaa cctatttaag caggcaaaat gaagaaatac
    37021 caacaattat ttaagcaaat ccaagaaacc attcaaaacg agacttacgc tgtcggagat
    37081 ttccttccta gcgagcacga ccttatggag caatatcaag tgagtcgtga taccgtccga
    37141 aaggccctgt ctctcctcca agaggaagga ttgatcaaaa agataagagg gcaaggttct
    37201 caagtcgtca aagaagaaac cgtcaatttc cctgtatcca acctaaccag ctaccaagaa
    37261 ctagttaaag aacttggact gcgctctaaa accaacgtgg tcagtctgga caagattatt
    37321 attgataaaa aatcctcact gataaccggt ttcccagagt ttcggatggt ttggaaggtg
    37381 ggccgccagc gtgtggtgga tgatctggta tccgttctgg atacggacta tctggatatg
    37441 gaactcatcc caaatctcac tcgccaaatt gctgagcagt ctatctattc ttatatagaa
    37501 aatggcctca aactccttat tgattatgct cagaaggaaa tcaccattga ccactcaagc
    37561 gaccgagaca agattctcat ggacattggc aaagaccctt atgtcgtttc gattaaatca
    37621 aaagtctatc tccaagacgg acgccaattt cagtttaccg aaagtcgcca taagttagag
    37681 aaatttagat ttgtagattt tgcaaaacgc aagaaataaa agactgagac accagatctc
    37741 agcctttttc ggctctataa tatttgtagt gggtaaatcc cttatagata ttatggagcc
    37801 tattttgtta tagaaaaaaa gtcccatatg acctataatg aaaagcgaca aaaccatcat
    37861 tagaaagatt catatggaac aattacattt tatcacaaaa ctgctcgata ttaaagaccc
    37921 aaacatcaag attctagata tcatcaatat ggatacccac aaagaaatta tcgctaagct
    37981 ggattatgag gctccatctt gccctgattg tggaagtcta atgaagaaat atgactttca
    38041 aaaaccgtct aagatccctt acctcgaaac aactggtatg ccttctagaa ttctccttag
    38101 aaaacgccgt ttcaagtgct atcactgttc aaaaatgatg gttgctgaga ctcccctggt
    38161 aaagaaaaat caccaaatcc ctcgtatcat caaccaaaaa attgctcaaa agttaattga
    38221 aaaaatgtct atgactgata ttgcccatca gctttccatt tcaacttcaa ctgtcattcg
    38281 caagctcaat gactttcact ttgagcatga tttttctcgg cttccaaaga ttatgtcttg
    38341 ggatgagtat gccttcacta agggaaagat gagtttcatt gcgcaagatt ttgataatct
    38401 taatattatc actgttcttg aaggcagaac acaagctgtc atccgaaatc actttcttcg
    38461 ctacgataga gccgttcgtt gtcaagtgaa aatcattacg atggatatgt ttagtcctta
    38521 ctatgacttg gctaaacag
//
LOCUS       DAVKDZ010000011        36074 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000011 DAVKDZ010000000
VERSION     DAVKDZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36074
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.011"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            138..264
                     /locus_tag="VWZ30_000563"
                     /pseudo
     CDS             138..264
                     /locus_tag="VWZ30_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915902.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator T protein"
     gene            259..2196
                     /locus_tag="VWZ30_000564"
     CDS             259..2196
                     /locus_tag="VWZ30_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEU9334363.1"
                     /translation="MDYSKVAAEVIEAVGKDNLVAAAHCATRLRLVLKDEAKVNQAAL
                     DNNADVKGTFSTNGQYQIIIGPGDVNFVYAEIIKKTGLKEVSTDDLKEIANKDKKFNP
                     LMDLIKLLSDIFVPIIPALVAGGLLMALRNFLTSPDLFGPQSIEDMYPAIKGFSAMIQ
                     LMSAAPFMFLPVLVGISAAKRFGANQFLGAAIGMIMTTPDLGGKEAFWDILGFHVTQT
                     NYAYQVIPVLVAVWLLANLEKFFHKKLPSAVDFTFTPLLSVMITGFLTFTVIGPVMLV
                     VSDAITNAIVWLYNTTGAFGMGLFGGTYSLIVMTGLHQSFPAIETQLLSAYNNNGTGF
                     GDYIFVVASMANVAQGAATLAVYFLTKNAKTKGLSSSAAVSAFLGITEPALFGVNLKY
                     KFPFFCALAGSAIGAFVAGLTHVIAVSLGAAGFIGFLSIKAGSIPMYIIAEIMSFVAA
                     FAFTYFYGKTKAASVFADEAATATAETVTEPTVEAPVVEETDTLQNETLVTPIVGDVV
                     ALADVNDPVFSSGAMGQGIAVKPSQGVVYAPADAEVSIVFPTGHAFGLKTRNGAEVLI
                     HVGIDTVSMNGDGFEAKVAQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYA
                     SVAPVATGSVAKGDAVIEVKI"
     gene            2345..3232
                     /locus_tag="VWZ30_000565"
     CDS             2345..3232
                     /locus_tag="VWZ30_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9334364.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLAYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGENIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(3275..3892)
                     /locus_tag="VWZ30_000566"
     CDS             complement(3275..3892)
                     /locus_tag="VWZ30_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEU9334365.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQMI"
     gene            complement(4307..4495)
                     /locus_tag="VWZ30_000567"
                     /pseudo
     CDS             complement(4307..4495)
                     /locus_tag="VWZ30_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(5005..5085)
                     /locus_tag="VWZ30_000568"
                     /pseudo
     CDS             complement(5005..5085)
                     /locus_tag="VWZ30_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            5202..6095
                     /locus_tag="VWZ30_000569"
     CDS             5202..6095
                     /locus_tag="VWZ30_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9334366.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            6092..7291
                     /locus_tag="VWZ30_000570"
     CDS             6092..7291
                     /locus_tag="VWZ30_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9334367.1"
                     /translation="MRNMWVVIKETYLRHVKSWSFFFMVISPFLFLGISVGIGHIQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETLLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILVHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMASRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(7644..8411)
                     /locus_tag="VWZ30_000571"
     CDS             complement(7644..8411)
                     /locus_tag="VWZ30_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334368.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(8428..9123)
                     /locus_tag="VWZ30_000572"
     CDS             complement(8428..9123)
                     /locus_tag="VWZ30_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9334369.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            complement(9128..9493)
                     /locus_tag="VWZ30_000573"
     CDS             complement(9128..9493)
                     /locus_tag="VWZ30_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9334370.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     SVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            9633..10106
                     /gene="nrdR"
                     /locus_tag="VWZ30_000574"
     CDS             9633..10106
                     /gene="nrdR"
                     /locus_tag="VWZ30_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEU9334371.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            10107..11276
                     /locus_tag="VWZ30_000575"
     CDS             10107..11276
                     /locus_tag="VWZ30_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEU9334372.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLRHPVYRRLLEKKIGDVAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPISSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRER
                     GQKNQAQKSSTPSPAKSNVPKWSNPDYKNETSEETRLELERKKQELLARLEKGGD"
     gene            11277..12173
                     /gene="dnaI"
                     /locus_tag="VWZ30_000576"
     CDS             11277..12173
                     /gene="dnaI"
                     /locus_tag="VWZ30_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEU9334373.1"
                     /translation="MESVGDVLKRQPSRFHYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVMNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKQASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAIGDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEELELHFAKGKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            12170..12883
                     /locus_tag="VWZ30_000577"
     CDS             12170..12883
                     /locus_tag="VWZ30_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEU9334374.1"
                     /translation="MTETIKLMKAHTSVCRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            12897..14207
                     /gene="der"
                     /locus_tag="VWZ30_000578"
     CDS             12897..14207
                     /gene="der"
                     /locus_tag="VWZ30_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU9334375.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIHEQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            14298..14633
                     /locus_tag="VWZ30_000579"
     CDS             14298..14633
                     /locus_tag="VWZ30_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334376.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            14773..15108
                     /locus_tag="VWZ30_000580"
                     /pseudo
     CDS             14773..15108
                     /locus_tag="VWZ30_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15293..16084
                     /locus_tag="VWZ30_000581"
     CDS             15293..16084
                     /locus_tag="VWZ30_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334377.1"
                     /translation="MKIPLLTFARHKFVYVLLTLLFLTLVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPITIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAILTHFFGTFSPLGWNSLFSDGSSLQR
                     LLDGEITSYLFFTCVLLIGIFINAVYFFKIVDYLGNVTRSAIAYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            16077..16718
                     /locus_tag="VWZ30_000582"
     CDS             16077..16718
                     /locus_tag="VWZ30_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334378.1"
                     /translation="MFKNFNNILLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FLDYTRKRLVVISKFFTQDLFVWFLGLLPIGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLIVSRLEEKFKKG"
     gene            16722..17372
                     /locus_tag="VWZ30_000583"
     CDS             16722..17372
                     /locus_tag="VWZ30_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9334379.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            17383..17670
                     /locus_tag="VWZ30_000584"
     CDS             17383..17670
                     /locus_tag="VWZ30_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334380.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLTFFFFPWDRAILGVRIDIFI
                     IQKVLLVFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            17816..20329
                     /gene="secA"
                     /locus_tag="VWZ30_000585"
     CDS             17816..20329
                     /gene="secA"
                     /locus_tag="VWZ30_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU9334381.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQAMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            20410..21441
                     /locus_tag="VWZ30_000586"
     CDS             20410..21441
                     /locus_tag="VWZ30_000586"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9334382.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYARRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            21443..22474
                     /locus_tag="VWZ30_000587"
     CDS             21443..22474
                     /locus_tag="VWZ30_000587"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9334383.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEVFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            22513..22881
                     /locus_tag="VWZ30_000588"
     CDS             22513..22881
                     /locus_tag="VWZ30_000588"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU9334384.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            22871..23974
                     /locus_tag="VWZ30_000589"
     CDS             22871..23974
                     /locus_tag="VWZ30_000589"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU9334385.1"
                     /translation="MKASPHRPTKVLIHLGAIRQNIQQMGAHIPQGTLKWAVVKANAY
                     GHGAVAVAKEIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            23993..26008
                     /gene="recG"
                     /locus_tag="VWZ30_000590"
     CDS             23993..26008
                     /gene="recG"
                     /locus_tag="VWZ30_000590"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU9334386.1"
                     /translation="MNLHQPLHVLPGVGLKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRVQGSGLVLNWSQEKVTAVKASLPFALTQAQEKSLQEILIDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTAHFAGKAEVALLHGKMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVVSYISSIEGWQEDPEWRMIAFHLEKKEHLD"
     gene            26329..27285
                     /locus_tag="VWZ30_000591"
     CDS             26329..27285
                     /locus_tag="VWZ30_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU9334387.1"
                     /translation="MKNPALLEEMKIYLGRDEVPEDVDAFWDEEVKKVSTLPAYQLEE
                     INFHISQVKCYELTFEGSKQGKVYARVVLPKSEEKVPLIFHFHGYMGRGWDWADMLAF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAVEGRDHLFYKDVYLDIYQL
                     VEIIASLPQVDEKRLSSYGASQGGALALVAAALNPRIQKPVAIYPFLSDFRRVLEIGN
                     TSEAYDELFRYFKFYDPFHETEEEIMAILAHRIKGEVKMITGLDDDVCYPITQFAIYN
                     RLTCDKAYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            28048..28275
                     /locus_tag="VWZ30_000592"
     CDS             28048..28275
                     /locus_tag="VWZ30_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334388.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            28391..31274
                     /locus_tag="VWZ30_000593"
                     /pseudo
     CDS             28391..31274
                     /locus_tag="VWZ30_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285043.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
     gene            31946..35512
                     /locus_tag="VWZ30_000594"
     CDS             31946..35512
                     /locus_tag="VWZ30_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU9334389.1"
                     /translation="MFLYLKGLGKYRKSKAYGLVGCLALVSMIGLAAPDLPVIGGGVV
                     AADVIQGGNDIKDVNVHSKSAEGVAMTYTTYDSGTSGKQTASGSGVFVAPNVMVTVAH
                     NYYDKNQEDKSAVLRGGASARSYVVMNSETEKHNKVPTSGVSETLEKDSIHLYDEKNF
                     GKDYSNDLAVVVTKKTVEAMTGGEDSPRELSHKEVSTGDKISMVGYPNDFSTPNLSAE
                     NKARLKDGKAYSVTTTVSSVNKESGTVTYHSSALGGFSGAPLFNDKGEVVGIHQHGTN
                     TPNAQESERIGGGTLFTEKHRAWIRSMIDKYGIKGWYIDGANRYYYDENHRALKDVES
                     EIDGALYRFDEKGRATLLEGEEKGRVLLRVEDTKGTPLISDKVVQEGSVGSGLNFHLR
                     QNPNFKQLIATSPTAKVVSYNGVPINKLASDTSWSDEYVSKLALGDTIIRAVVDSVTP
                     PSTSSSDFARTEVGKVDLSGKSNLPVPSKEVLQAPNGSENFYATTHIQTPDGSGSGTL
                     IAPNLVLTVAHNFLTVKGSEVVTKSGRTNTVYKATLPSGQPVNFSDDDIVYWNKKDSV
                     FGFKNDLALVRLKEKLTAVSPVEVVSESTSITKGDKVSVYGFPDGRLSPVLDSEVVAT
                     TDFGSGIEGISYGGTKPGASGGGLYNDKGSLIGVHQNGVVGSRSGGLVLSKEQLDWVR
                     SYIEGKPKAPVYVTDNILVDEKDKDKLPSTSKEEKPTTPKVESDKDKPNTPLKPQEKP
                     KTEVITSYEGDSTLEVGKERTEETEGEKEGVSLIYRTVYKGTKSKTEMSPIAFDTVYQ
                     GDETKELGFRSVLEGKEGLVTRTTSYQVDKYTGAVSSKISEEKIAPQSQVITLGIKKN
                     SSTKEVPITERFEDSAELEKGKTEVISEGSVGKEVTTVTYKVLPDGKVIENSRTVDVT
                     PMRERVVRKGVKEVVSPDKVESLVPKDAPIREEQPALSEGFSESDALVSGEKIQGDLG
                     ILIVSSEELVPERVEVPDFVTKVTGGEKLTVEGHRNESKIKTPSKQERSSRPETTAQF
                     TTNGTGSSSLTAVFGGKTDKILLSTVEHSVIKHNQQRGWHKINNQWYFRNSDGKERTG
                     WMKENDAWYYFDTNGTMQTGWLEDTDGNWYYLNDNGRMEIGWFQDSSGAWYYLGSSGR
                     MESNTWIYYKGKWYYIDALGKLLFNSVTPDGYRVNEYGEWIN"
     gene            35789..>36074
                     /locus_tag="VWZ30_000595"
     CDS             35789..>36074
                     /locus_tag="VWZ30_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9334390.1"
                     /translation="MILRHPGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAKNVTIL
                     EGSNIHVLPSMPYSAQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINRLW"
BASE COUNT        10940 a         6447 c         8096 g        10591 t
ORIGIN      
        1 aaatcaatca aatttttacg aaaatgtgaa agataatgaa atgaaagact ttttaaccaa
       61 ggtttcaaac ttatttttca agaaattcct gatgaaacag tcaaaaacaa tcaatctaaa
      121 gcttgcccac taagtagata gaatattttt tctgcaaaac gcttgacata tttttaaaac
      181 atgataaaat aatagtcgta agggcgaata aaatcgcttt caaacaagaa caaaatgtta
      241 tataaggaga tttttgctat ggattatagt aaagttgctg ccgaggtcat tgaggctgtc
      301 ggtaaggata atcttgtagc tgcagctcac tgtgcgacac gtcttcgctt ggtattgaaa
      361 gacgaagcaa aagtgaatca agcagctctg gataataatg cggatgtcaa ggggactttt
      421 agcactaacg gacaatatca aattatcatt ggtcctggtg atgttaactt cgtatatgcg
      481 gaaattatta agaaaactgg tttgaaagaa gtttcgacag atgatttgaa agagattgct
      541 aataaagaca aaaagttcaa tccattgatg gacttgatta aacttttgtc agacattttt
      601 gttcctatta tccctgcctt agttgcaggt ggtctcttga tggctttacg gaacttcttg
      661 acatcaccag atttgtttgg acctcaatca attgaagata tgtatcctgc tatcaaaggc
      721 ttctcagcta tgattcaatt gatgtctgct gctccattta tgttcctccc tgttttggtt
      781 ggtatttcag cagccaagcg ctttggagca aaccaattcc ttggtgcggc aatcggtatg
      841 attatgacca ctccagattt gggtggtaaa gaagccttct gggacatctt aggtttccat
      901 gttacacaaa caaactatgc ttatcaggtt attccagtcc ttgttgcagt ctggttgtta
      961 gctaatttag aaaaattctt ccacaagaaa ttgccatctg cagttgactt cactttcaca
     1021 ccgcttttat cagtgatgat tacaggattc cttaccttta cagttatcgg tcctgttatg
     1081 ttggttgtgt ctgatgcgat tacaaatgcg attgtatggt tgtacaacac aacaggtgcc
     1141 tttggtatgg ggctatttgg tggaacttac tcattaatcg ttatgacggg tcttcaccaa
     1201 tcattcccag caatcgaaac acaattgttg tctgcataca ataataacgg tactggattt
     1261 ggagactaca tctttgttgt tgcttcaatg gcaaacgtag cacaaggtgc ggctactctt
     1321 gcagtctact tcttgacaaa aaatgctaaa acaaaaggat tgtccagttc ggctgctgtt
     1381 tctgccttcc ttggtattac agaacctgct ctctttgggg taaacttgaa atacaaattc
     1441 ccattcttct gcgcccttgc tggttcagca attggtgcct ttgtagcagg tttaactcac
     1501 gttattgcag tttcattggg agcggctgga tttattggat tcctttctat taaggctggt
     1561 tcaattccta tgtatataat tgcggaaatt atgtcctttg ttgccgcctt tgctttcact
     1621 tatttctacg ggaaaacaaa agcagctagt gtctttgctg atgaagctgc cacagcaacc
     1681 gcagaaacag ttacagaacc tacagtagaa gcaccagtag tggaagagac tgatactctt
     1741 caaaatgaaa cacttgtaac tcctatcgtc ggtgatgttg tcgctcttgc tgatgtcaat
     1801 gacccagtct tctcaagtgg agctatggga caaggtatcg ctgtgaaacc aagccaaggc
     1861 gtggtctatg caccagctga tgctgaagtt tcaattgtct ttccaacagg gcacgctttt
     1921 ggtttgaaaa caagaaatgg tgctgaagtt ttgattcacg ttggtattga cactgtatct
     1981 atgaacggtg acggttttga agcaaaagtt gctcaaggta ataaggtgaa agctggcgat
     2041 gttcttggaa catttgactc aaacaaaatc gctgcagctg gacttgatga tacaacaatg
     2101 gttatcgtta caaatacagc tgactacgct tcagtagctc cagtcgcaac aggttcagtt
     2161 gcgaaggggg atgctgtgat cgaagtgaaa atctaatcaa tcctctctaa tgtgaaaacg
     2221 aacaaatgac atgtttgttc gtttttatta gatagtaaga tttacagagg aaaatctaaa
     2281 aaatagagac atttagactt tcgaagtatg ctataataaa gaaaataaaa acaagaggtt
     2341 tatcatgaca aaattatatg gaagcttgga agcgggcggt acaaagtttg tctgtgctgt
     2401 cggtgatgaa aactttaacg ttgtagaaaa aacacaattt ccaacaacaa ctccaatcga
     2461 aacaatcgat aaaaccattg agttcttctc aaaattcgat aaccttgctg gtcttgcagt
     2521 tggttcattt gggccgattg atattgacaa aaactcaaaa acttatggct ttatcacgac
     2581 gactccaaaa ccaaactggg caaatgtgga cttgcttggt gcccttcgtc gcgccctcaa
     2641 cgtgccaatg tacttcacaa cagacgtaaa cagctctgct tacggtgaag tggttgcccg
     2701 taacaatgct ggtggtcgta tcgaaaactt ggcttactac acaatcggta caggtatcgg
     2761 tgcaggcgtc atccaacgtg gtgagtttat cggtggtgtg ggtcaccctg aaatgggtca
     2821 ttattatgtt gctagacacc caatggatat tgaaaaagag tttaagggtg tttgtccttt
     2881 ccataaggga tgtctggaag gctatgcagc tggtccaagt ttggaagctc gtacaggtgt
     2941 acgtggggaa aatattgaac tcaacaaccc tgtttgggat gttcaagcct actatatcgc
     3001 tcaagctgcg gttaatgcga cagtgacttt ccgcccagac gtgattgtct ttggtggagg
     3061 ggtcatggct caacaacata tgctggaccg tgtccgtgag aaatttacat ctcttcttaa
     3121 tggttaccta ccagtaccag atgtgcgtga ctatatcgtg acgccagcag tcgcaggaaa
     3181 tggttctgcc acacttggga actttgttct tgcaaaagaa gtttcaaaat aaagcacaaa
     3241 atccgcttga gaaaccaaac ggatttttta cgtgtcaaat catttgccag aaaaagtcaa
     3301 taatataggt cagaccgtag gtataaacca cgagggtaaa gggtttggtc agaatgatga
     3361 tggtcatgta gcgcttgaaa ctcatcttgg tcagggcagc cagcatacag agaaagtcag
     3421 ctgggctaat gggccaaatc atcataaaaa taaagaagcg gtcaaaacga ttgcccttat
     3481 ctagccagcc gatgtacttg tcgtaggtgc gcttgctgac gacagactgg acaaaggcag
     3541 ctccgtatag gcgcactaga taaaagataa tggcacagcc aatcacgatg ccgatatagt
     3601 tgtagatagt cccgatgatg tgcccgtaga taaagacccc agccaccgag gtcaaggccc
     3661 ctggaatgat agggacgaca gtctgtaaaa tctgtaaaaa gataaagaga ggtggacccc
     3721 agatgcctgc ctgctggata aaggcagaga gggtttcctt ggattgtaaa atcccagcct
     3781 gataagccca aatacagaaa atcaaacttc caaccccacc gacaatcgat gagatattga
     3841 taactcgctg caaaagggga gaaacagctt tcatttgttt atcctgtgac atgtaaactc
     3901 ctcatagata gtatatttct actataccat attttggagg agaaaggggg aatgagtctt
     3961 ttttgataag gaatagaaag taaatagccc ttcgagactt agaataggtc tgtcaactaa
     4021 aggttatatg cattctaggt tttgaataat cgcaattcca taaatttaaa agcattctaa
     4081 gtttcgaata gcttatctga gataattttg aggcattcgc gctttcgaat agtcattttc
     4141 cctaaagttt agatgtattc tgaagctaga aagactaaaa tctaaaagta gtaagagtct
     4201 ctctctgatg gacgtaattt atggctagta tgaatccttt gaattgttag ttaattttaa
     4261 tattaatcag ttctgaaata attactaaat atagttagat ggtgaattaa ttattgctaa
     4321 atttcaagtt caagttagtc atccagaagt cttctctggg ggacttgtag ttcaagcatt
     4381 ttttaggata gttgttaatc cacttttcga tgaatgcgac ttcctttgta gtcgttttct
     4441 tagtttcctg aggtaaccat ctatgaatga gcctgttgtg attttcatta gatcctatag
     4501 gggtgtgcat aatagatata gcagattgaa actagaatag tatacatcta cttctaaaac
     4561 attgttagaa atcaatttga ctgtcctgat caatttgtcc tgttcttatt tcattttact
     4621 atataatcct tagaaaacat atcggacaag cggttgaatt ctgttccgtc cattagtaga
     4681 taaaggatta ctccttcttg aaatgacaga tgaacttgtt tcttataaaa atgttgtctg
     4741 aaactattag cctttttcca gaagttttag aggaagaaaa ctttacgagg aaaaaggagt
     4801 tactcaaata acaattcagg attaaaaata gacagttgag gagcggaagg tataaattaa
     4861 gtttgctgct gtataatgga tttatcacta atactcttcg aaaatctctt caaaccacgt
     4921 cagcttcgcc ttgccgtaga tatagttact gacttcgtca gttctatcca caacctcaaa
     4981 acagtgtttt gagctgactt cgtcagtttt atctgcaacc tcaaagctgt actttgagca
     5041 gtctacggct agcttcctag tttgctcttt gattttcatt gagtataaaa gtgctccagg
     5101 ccatatttta cacagcaata gtcttctaaa aatggacaca aaacgttgta aaggttatca
     5161 aaaaggagta taatgagtgc aagaaatttc ggaggtgaaa gatgctagaa gtaagaagtc
     5221 tagagaaaag ttttggatcc aagcaagttt tgtttggtat tgactttcaa gcgcgaccag
     5281 gtcgtatttt gggactagtc ggaaaaaatg gtgctggaaa aacaacgatt ttccatagta
     5341 ttttaaaatt cctagaatat cagggagaaa tcggtctgga tggtcaggat attcgtcagg
     5401 agacctatgc tcggattggt tatctgcctg aagaacgcag tctcatgcct aaattgacag
     5461 ttcttgaaca agttcgctac ttggcgactc taaaaggcat ggatgccaaa gaagtcaaag
     5521 aaaaactccc tcaatggatg aagaggttgg aagtgaaagg aaagctgaca gataaaatca
     5581 agagtctgtc aaaaggaaat cagcagaaga ttcagctcat tattactctg attcatgaac
     5641 cagacctgat tatcttggat gagcctttta gtggattgga cccagttaat acagaattgc
     5701 tcaaacaagt catttttcag gaaaaagagc gcggagcaac cattatcttt tctgaccatg
     5761 tcatgaccaa tgttgaggaa ctttgtgacg atattctgat gatccgagat ggccgtgtgg
     5821 tcttgcacgg accagttcag gatgtccgca atcaatacgg gaaaacgcgt ctctttgttt
     5881 ccagtgaacg aagcaaggaa gaactggaaa atcttcctca tgtcaaacag gtgagcttga
     5941 ccaaacaagg cagttggaaa ttgattttgg aggatgagag cgctggaagg gaactcttcc
     6001 caatcttgac tcaaggtcaa tatatcgcaa catttgacca acaagcgcca actatcgatg
     6061 aaatctttaa actagaatca ggagtggaag tatgagaaat atgtgggttg taatcaagga
     6121 aacctatctt cgacatgtca agtcatggag tttcttcttt atggtgattt cgccgttcct
     6181 ctttttagga atctctgtag gaattgggca tatccaaggt tcttctatgg ctaaaaataa
     6241 taaagtggca gtagtgacaa cagtgccatc tgtagcagaa ggactgaaga atgtaaatgg
     6301 tgttaacttc gactataaag acgaagcaag tgccaaagaa gcaattaaag aagaaaaatt
     6361 aaaaggttat ttgaccattg atcaagaaga tagtgttcta aaggcagttt atcatggcga
     6421 aacattgctt gaaaatggaa ttaaatttga ggttacaggt acactcaatg aactgcaaaa
     6481 tcagcttaat cgttcaactg cttccttgtc tcaagagcag gaaaaacgct tagcgcagac
     6541 aattcaattc acagaaaaga ttgatgaagc caaggaaaat aaaaagttta ttcaaacaat
     6601 tgcagcaggt gccttaggat tctttcttta tatgattctg attacctatg cgggtgtaac
     6661 agctcaggaa gttgccagtg aaaaaggcac caaaattatg gaagtcgttt tttctagcat
     6721 aagggcaagt cactatttct atgcgcggat gatggctctg tttctagtga ttttaacgca
     6781 tattgggatc tatgttgtag gtggtctggc tgccgttttg ctctttaaag atttgccatt
     6841 cttggctcag tctggtattt tggttcactt gggagatgct atctcactga ataccctgct
     6901 ctttattttg atcagtcttt tcatgtacgt agtcttggca gccttcctag gatctatggc
     6961 ttctcgtcct gaggactcag ggaaagcctt gtcgcctttg atgattttga ttatgggtgg
     7021 tttttttgga gtgacagctc taggtgcagc tggtgacaat ctcctcttga agattggttc
     7081 ttatattccc tttatttcga ccttctttat gccgtttcga acgattaatg gctatgcggg
     7141 gggagcagaa gcatggattt cacttgctat tacagtgatt tttgcggtgg tagcaacagg
     7201 atttatcgga cgcatgtatg ctagtctcgt tcttcaaacg gatgatttag ggatttggaa
     7261 aacctttaaa cgtgccttat cttataaata gaagagcctt gcgaaagcga ggtttttaaa
     7321 ggtgaaaagg gtggaatcac gaacctaatt atgtacacat tatgtaaaca ggttgcttga
     7381 aaactcaatt gtatagtgaa ctgaatctgg aataggataa tattacttct aaaacattct
     7441 tataaattga tttgaattcc aaaatcccat cgttccgttc ttatttcatt ttgcgatata
     7501 gtgaaaggaa ataagtcagc tcacaaaaaa cgcataatat caaggatttt taacacctga
     7561 tactatgcgt tttctaattt taaagacttt ttgcctggtc tcttttgttc tatgagtatg
     7621 ttacatatct aggtaaaaat tatttaaagc aaattaacct tatttctcaa gatgtagtag
     7681 gttccgagat aaaagatagc tatgaatatg agttctttaa caatggctat acctgctccc
     7741 atatagaaat ggtcattgag tcctaccagt gaattgacat agaaattaga actaagattg
     7801 aagaaaagtt caataaatcc aatgacgatt tggataccaa tgtaggctgc aacagcgagt
     7861 gctgtacggt attcattgaa aagctgtcca atggaaatag ccaggtagat gcataggatt
     7921 cctgaaatgg tatttagtag gaaggatata cctgtaagaa agatctgagg gagatgtgtt
     7981 tctacaaatg taatcacata agaaagagga atccattctg gagctgttaa agccagaata
     8041 ataacagcac ttagagccaa tacagcggtg ctaatcaatg accagataaa ggcaccgatt
     8101 agtttggctg tgatgatatg gtgttcagaa actggcaagg tcaaagtcag atagccttgt
     8161 cggtcgtaga cactaccttt gaagcgttta ataatcaaga aaatggttga aatcgcaagt
     8221 gtaagcatca agccaccaaa gactgtagct agaaaaagta gcatagtagc ctgactttct
     8281 tggtaaggta gatttttaaa gccttgtgtc tgtattccga tgagggcaga aaggactagc
     8341 acggctgcgt agagggctaa ataccacttg ttaacatttt taaattcgta gtgaactaaa
     8401 ttccaaaaca taaataatct cctttgctta ggccttaaat tcctgacgga agagttggtc
     8461 aatggattca cctgactcgt agcgaatatc atctacattt ccttgacgga cgacttttcc
     8521 gtcttttagg aagacaattt catccaagat tggctcgata tcagaaatca agtgggtaga
     8581 aatcaaaacg gtagaagttg gtgagtagtt gttgataatg gtattgagga tataagcacg
     8641 ggctgctgga tccaccccac caatgggttc atccaaaaca tagagacgag catcacggct
     8701 cataaccaaa atcagttgaa ccttttcttt gtttcctttt gatagtttct tgagacgact
     8761 attttcatca atgcccaggt ctgcaagtag atgatgagcg cgttcaagat tgaaatcttt
     8821 atagaaggtc ttgaagtagg ttagggcttc tttgaccttc atttgctcat tgagataggt
     8881 cgtatcaggc aaataagcta caacggcctt ggttgctggg cttgggtcca tgtcgttgat
     8941 gaggacacgt ccttgatctg gttgtaagag gccattaatt agtttaatca gggttgtttt
     9001 tcctgagccg tttggcccaa gaaggccgac aatttttcca gctggaatgt caagagaaac
     9061 attttcaagg gctggtgttg ctccatatga tttggataca ttttcaaatg ctagtaatga
     9121 cataggctta aactccttta atataatcac tgactacgcc tggtagttct tctttttcat
     9181 agccaaaatg ggtcatggag gaaacgaagt gttccaattc ttcttctgat aattgtttgc
     9241 gtgactgagc gattagctcc ttatccttag tcacaaatcg tccagttgtt cgcttgctgt
     9301 agacaaatcc ttctcgttca aggtctgata aggctctttg gatggtattg ggattgacac
     9361 tagcctcgct agctagctcc ctcacggttg gaagttgttg attgggttcc agtgtatggg
     9421 aaacaatctg aagtttgatt ttctccataa tctgtaaata gatgggtttt ttgttgtcaa
     9481 atgtccagga catcttagtc tcctttctct tattcttttg tcttaattaa ataatacaac
     9541 aaaacaaaaa acttgtcaag caaaaagaag aattgttttg ggaatcgctt aaaaaatggt
     9601 ataatgaaaa gaaaacgata aggagggaaa ggatgcgttg tccaaaatgt ggggctacca
     9661 agtcaagtgt tatcgatagt cgccaagcag aagaagggaa caccattcgt agaagacgtg
     9721 agtgcgacga atgccaacac cgttttacaa cttacgaacg agtagaagaa agaaccttag
     9781 tggttgttaa aaaagatggc acacgggaac aattctccag agataaaatc tttaatggga
     9841 ttatccgctc agcccagaaa cgtcctgtgt caagtgatga aatcaacatg gtagtcaatc
     9901 gtatcgaaca gaaactccgt ggtcgaaatg aaaatgaaat tcaaagtgag gacattggtt
     9961 cactcgtcat ggaggagttg gctgaattgg acgagattac ctatgtacgt tttgctagtg
    10021 tctatcgtag ttttaaggat gtcagtgagt tagagagctt gctccaacaa atcacccagt
    10081 cctctaaaaa gaaaaaggaa agataaatga agccaattga ccgtttttct tatctaaaga
    10141 ataatcgggt gtcgcaagat acctcatctc tggtacagtg ctacctcccg attatcggtc
    10201 aggaggcact gagcctttat ctttatacaa tcagtttttg ggataatggt aggaaggaat
    10261 atcttttttc aagtatcctc aatcatctca actttgggat ggatagactg ataaaatcat
    10321 tgaaaatctt atctgctttt aatctcttga ccctctatca aaagggggat gtttatcagc
    10381 tagccctcca tgctcctcta tctagtcaag acttcttgag gcatcctgtt tatcgcagac
    10441 tcttagagaa aaagattggc gatgtagctg tggaggattt gaaagttgaa agtgctgatg
    10501 gtgaagaaat acctgtctca ctcaatcaag tctttccaga attggcagaa ctaggcagtc
    10561 aagaagacct tggtctcaag aagaaagtgg ccaatgattt tgacttggaa cattttcgcc
    10621 agctgatggc tcgagatgga cttcgttttg cggatgagca gtccgatgtc ttaaacctct
    10681 ttgctattgc cgaggagaag aaatggactt ggtttgaaac ctatcaattg gccaagtcaa
    10741 cagctgtttc tcaggttatt tcaaccaaac gcatgcgtga aaaaattgct caaaaaccaa
    10801 tttcctctga ctttagtctt aaggaaacaa ccattatcaa agaagccaaa agtaaaactg
    10861 ccctgcagtt cttggcagaa atcaagcaaa cacgcaaggg aaccattacc caaacagaaa
    10921 gagaactctt gcaacagatg gctggcttgg gcttgctgga cgaagtcatc aatatcattc
    10981 ttttattgac ctttaataag gtggattcgg caaatatcaa tgagaaatat gccatgaagg
    11041 tagccaatga ctatgcctat caaaagatac attcggcaga agaggcagtc ttgcgcatcc
    11101 gtgagcgtgg gcagaagaat caggctcaaa aaagcagtac ccctagtcct gccaagtcca
    11161 atgtacccaa gtggagcaat ccagattata aaaatgaaac cagcgaggaa actcgtctgg
    11221 aactagaacg taagaaacaa gaactattag ctcgattaga aaaaggagga gattagatgg
    11281 aaagtgtcgg agacgtactc aagcgtcaac ctagccgttt tcattatcaa gatttggtcc
    11341 agaaaatcat gaaggaccct gatgttgcgg cctttatcca gcaagaatcc ttgaccccag
    11401 aggaattgaa tcgcagtatc tccaagttta atcagtacat caccgagcgc gacaagtttc
    11461 tccgtggtga tacggattat attgccaaag gctacaagcc gattttggtt atgaatcatg
    11521 gctatgcgga tgtttcttat gaagaaactc ctgagctaat cgcagcggaa aaagaagcgg
    11581 ctattaagaa ccgtctcaag ttaatcaatc tgccagccag tctcaagcaa gctagtttgg
    11641 ctcaagtaga cttggatgat ttggggcgct taccagtttt tgaaaagtta ttagcattcg
    11701 tggagcaata tccagctatt cgaaaaggtc tttacttgta tggagacttt ggtgtgggta
    11761 aaagtttcat ggtggctgcc ttagctcatg atttatcaga aaaacgtggt gtttcctcca
    11821 ctcttctcca ctatcctagc tttgtcattg atgtcaaaaa tgctatcggt gatggtaatg
    11881 tcaagacctt ggtggatgag attaagcttt ctgaagtcct gattttagat gatattggtg
    11941 ccgagcaatc aacagcttgg gtgcgtgatg aaatcctgca ggtcattctc caatatcgga
    12001 tgcaggaaaa tttaccgacc tttttcacct ccaacttcaa ctttgaagaa ttggagctgc
    12061 atttcgctaa agggaagcat ggaaatgacg aaacctggga agccagacgc gtcatggaac
    12121 gcatccgtta tttggctgag gagactcgtt tagaaggagt aaaccgtcga tgacagaaac
    12181 cattaaattg atgaaggctc atacttcagt gtgcaggttt aaagagcaag aaattcccca
    12241 agtagactta aatgagattt tgacagcagc ccagatggca tcatcttgga agaatttcca
    12301 atcctactct gtgattgtgg tacgaagtca agagaagaaa gatgccttgt atgaattggt
    12361 acctcaagaa gccattcgcc agtctgctgt tttccttctc tttgtcggag atttgaaccg
    12421 agcagaaaag ggagcccgac ttcataccga caccttccaa ccccaaggtg tggaaggtct
    12481 cttgattagt tcggtcgatg cagctcttgc tggacaaaac gccttgttgg cagctgaaag
    12541 cttgggctat ggtggtgtga ttatcggttt ggttcgatac aagtctgaag aagtggcaga
    12601 gctctttaac ctacctgact acacctattc tgtctttggg atggcactgg gtgtgccaaa
    12661 tcaacatcat gatatgaaac cgagactgcc actagagaat gttgtctttg aggaagaata
    12721 ccaagaacag tcaactgagg caatccaagc ttatgaccgt gttcaggctg actatgctgg
    12781 ggcgcgtgcg accacaagct ggagtcagcg cctagcagaa cagtttggtc aagctgaacc
    12841 aagctcaact agaaaaaatc ttgaacagaa gaaattattg tagaaagtga gaaattatgg
    12901 ccctaccaac tattgccatt gtaggacgtc ccaatgttgg gaaatcaacc ctatttaatc
    12961 ggatcgctgg tgagcgaatc tccattgtag aagatgtcga aggagtgaca cgtgaccgta
    13021 tttatgcaac gggtgagtgg ctcaatcgtt cttttagcat gattgataca ggaggaattg
    13081 atgatgtcga tgctcctttc atggaacaaa tcaagcacca ggcagaaatt gccatggaag
    13141 aagcagatgt tatcgtcttt gtcgtgtccg gtaaggaagg aattaccgat gcagacgaat
    13201 acgtagcccg taagctttat aagacccaca aaccagttat cctcgcagtt aacaaggtgg
    13261 acaaccctga gatgcgaaat gatatctatg atttctatgc cctcggtttg ggtgaaccac
    13321 tgcctatctc atctgtccat ggtatcggta caggggatgt actagatgcg attgtggaaa
    13381 accttccaaa tgaatatgaa gaagaaaatc cagatgtcat taagtttagc ttgattggtc
    13441 gtcctaacgt tggaaaatca agcttgatca atgctatctt gggagaagac cgtgttattg
    13501 ctagtcctgt tgctggaaca actcgtgatg ccattgatac ccactttaca gatacagatg
    13561 gtcaagagtt taccatgatt gatacggctg gtatgcgtaa gtctggtaag gtttatgaaa
    13621 atactgagaa atactctgtt atgcgtgcca tgcgtgctat tgaccgttca gatgtggtct
    13681 tgatggtcat caatgcggaa gaaggcattc gtgagtacga caagcgtatc gcaggatttg
    13741 cccatgaagc tggtaaaggg atgattatcg tggtcaacaa gtgggatacg cttgaaaaag
    13801 ataaccacac tatgaaaaac tgggaagaag atatccatga gcagttccaa tacctgcctt
    13861 acgcaccgat tatctttgta tcagctttaa ccaagcaacg tctccacaaa cttcctgaga
    13921 tgattaagca aatcagcgaa agtcaaaata cacgtattcc atcagctgtc ttgaacgatg
    13981 tcatcatgga tgccattgcc atcaacccaa caccgacaga caaaggaaaa cgtctcaaga
    14041 ttttctatgc gacccaagtg gcaaccaaac caccaacctt tgtcatcttt gtcaatgaag
    14101 aagaactcat gcacttttct tacctgcgtt tcttggaaaa tcaaatccgc aaggcctttg
    14161 tttttgaggg aacaccgatt catctcatcg caagaaaacg caaataaaaa agtagaatct
    14221 ggaatgacag ttccggattt ttttgatata ataaaataat agaaaacgct atcaaaagaa
    14281 ggagggtgat gtcggtgatg gaacatttgt ttaaattctt acttttagca ccgtactttt
    14341 attttgataa ctggattgag aaggccaaca gaaatagtaa gttttttcca attttttatt
    14401 atttttactg gttttacatc cccttctatt ctctttttag ccttgcttgg acagttgttt
    14461 cagttctgtt tttcaatacc gtcttgagaa atgtgacaga tatcaagtta tggggcattt
    14521 ggtttctttt tattctgcta gctattggta tgaattggtt aacttattcc tgtttcaaag
    14581 aaatgtttcg cttgagacag gaactaggga agtctaaggg tggaaggcat tgatttatat
    14641 cggtttctat ttgtaggagg tggcttatgg tacctctaaa atcatttatt acacgctatt
    14701 ccaaggttta tattggtaat aacatttaat agaacgaaac taaagaaaac aatttaaaaa
    14761 aggaggcttg tgatggaaca cttgtttaaa atgataatct tatcaccctg tttttacttt
    14821 ttcagctgga ttgacaagga taatagagaa agtaaatttt tcccaatttt ttactatttt
    14881 tactggattt acatcaccct ctatgctctt tttagccttg cttagacagt tttttcagtt
    14941 ctgtttttca atatcgtctt gagaaatttg acagatatca agttatgggg tatttggcta
    15001 ttattactac ttatcgcttt tgctagtgac tggttagact atatctgttt caaaaaaatg
    15061 cttgacttga gacgggaact agggaagtct aaaggcggaa ggcattgatt tatactcttc
    15121 gaaaatctct tcaaaccacg tcaacgtcgc cttggattat atatgtaact gacttcgtca
    15181 gtcttatcta caacctcaaa gcagtgcttt gagcaacttg cggctagttt cctagtttgc
    15241 tctttgattt tcattgagta ttatattact ttctatttgt aggaggtggc ttatgaagat
    15301 tcctctctta acttttgcaa ggcataaatt tgtttatgtc ttgcttactt tgctttttct
    15361 tactttggtt tatcgtgatg ttttgatgac ttatttcttt tttgatattc atgcgcccga
    15421 tctagctaaa ttcgatggac aagcaattaa aaatgactta ttaaaatcag cattagattt
    15481 tcgtattctc cagttcaatc taggttttta tcaatcattt attattccaa tcaccattgt
    15541 tttgctaggt tttcaatata ttgagctgaa aaataaagtt ttacgattga gtattggaag
    15601 agaagtgagc tatcaagggc taaaaagaaa gttgactttg caagttgcaa gtatcccttg
    15661 tttaatatat ttagtgactg tgctgataat tgcaatttta actcatttct ttgggacttt
    15721 ttctcctctt ggatggaatt ctctattttc tgacgggagt agtttacaaa ggctcctaga
    15781 tggagagata acaagctatt tgttctttac ttgtgtccta ctaatcggta ttttcatcaa
    15841 tgcagtctat ttttttaaaa tagttgatta tttaggaaat gtgactcgtt cggcaatcgc
    15901 ctatttgatg tttctttggc ttggttctat gttactctat agtgccttgc cttactatat
    15961 ggttcctatg acgagtttga tgcaagctag ctatggggat gtaagtttga tgaaactctt
    16021 tactccttat atcctttata ttgtccctta tatggttctt gaaaaatatg aagataatgt
    16081 ttaagaattt taacaatatt ttgctaaaga gaaagattgt tttactactt cgtatagttc
    16141 tgatgatgat tttgataaac tatctattgt caacagcggt tcaaaagcag gatgctgtta
    16201 tctttttcaa gagagaattg atttcaatct tttcctataa tgactattct gaagcgaatt
    16261 tagaaatccc caaactcttg ttaaaccttt cgcttttcat ggtaggatgg ctctctgtca
    16321 ttttacttga aagtgatttg gcagatcatt accatcactt gattcgctat caatcaagct
    16381 cctttttaga ttatacaagg aaacgattgg ttgtcatttc taaatttttt actcaagatt
    16441 tgtttgtctg gtttcttggt ttacttccta taggaattca tttcaaaaca gtcgcacttt
    16501 tctttttact tgctcagtta atgatgttgt acttactact gtcttatctg atagcactga
    16561 ttagtgcggg cgctggtttt tccttttttc tctatttttt agcatttgtg ggacaagaat
    16621 ggatgatgga tcatattgta acagtgtatt tagtactttt aagtttatta gttatgttga
    16681 ttgttagtcg cttggaagag aaatttaaga aaggataaac gatgagactt gaaattataa
    16741 atggacagaa aatttatggg aaaagaccta ttttaaatca gttgaatttg gtgtttcaat
    16801 caggaaaaat ttatggactt aaaggtgata atggatctgg caagacggtt cttttaaaga
    16861 tacttgctgg ttatattaag cttgacaaag gaaaagttct tcaagacggg aaagtctacg
    16921 ggataaaaaa tcattatatt caggatgcag gaattttaat tgaaaaagtc gagtttttat
    16981 ctcatttatc cctgagagaa aatttggaac tgttaaggta tttttcatct aaagttacgg
    17041 aaaaaagaat tgcctattgg attcaatact atgatttaca ggaatttgaa gacgttgaat
    17101 accgtcattt atccttagga acaaagcaaa aaatggcctt gattcaagcc tttatttcct
    17161 ctccttctat actctttctc gatgaaccta tgaatgcttt ggatgagaag agtgtgaggt
    17221 taaccaaaca ggtcatttta tcttacctga aaaaagaaaa tggtctggtt atcctgacgt
    17281 cgcacatatc ggaagatatt tcagaccttt gtacagatgt attagttgtc gaaaatggac
    17341 atatacaata tgtaaaggat atacaatcct aggagatggc ttatggcaca tctaaaatca
    17401 tttattacac gctattccaa ggtttatatt ggtttagttc tgctgatctg gctgactttc
    17461 ttctttttcc cttgggatag agcgattctg ggggtaagga ttgacatctt catcatacag
    17521 aaagtcttgc tagtttttgg aattctgtcc attctcatgg ccttgctgtc caagaaagtc
    17581 agtctctttg tttttggact gatttgctgt ctttctcttt ggattaactt atttatcaca
    17641 tttgccattt tgccgatttt tggcaattaa acagtcataa aagtcggaga ggttagcttg
    17701 aaaactaacc tctttttcct tttcaaaatg gggattcttc cttgaaaata atcagtaatt
    17761 gtgctaaaat taaaggaaca ttctaaaata ttcggaattt aaagtaagga aaaacatggc
    17821 taatatttta aaaacaatta tcgaaaatga taaaggagaa atccgtcgtc tggaaaagat
    17881 ggctgacaag gttttcaaat acgaagacca aatggctgct ttgactgacg accaactaaa
    17941 agcaaaaaca gttgaattta aggaacgtta tcaaaatgga gaatcactgg attcattgct
    18001 ttacgaagca tttgcggttg tccgtgaagg tgccaaacgt gtcctaggtc tcttccctta
    18061 taaggttcag gtcatggggg ggattgttct tcaccatggt gacgtgccag agatgcgtac
    18121 aggggaaggg aaaaccttga ctgcgaccat gccggtatac ctcaatgccc tttcaggtaa
    18181 aggggttcac gtagttacgg ttaatgaata cctgtcagaa cgtgacgcga ctgagatggg
    18241 tgaattgtac tcttggcttg gtttgtcagt agggattaac ttggctacca aatctccaat
    18301 ggagaaaaaa gaagcctatg agtgtgatat tacttactca actaactcag aaatcggatt
    18361 tgactacctt cgtgacaaca tggtcgttcg cgctgaaaac atggtacaac gtccgcttaa
    18421 ctatgccttg gtcgatgagg ttgactctat cttgattgac gaggctcgta cacctttgat
    18481 tgtatcaggt gccaatgcgg ttgaaaccag tcagttgtat cacatggcag accactatgt
    18541 aaaatctttg aacaaagatg actacatcat cgatgtgcag tctaagacta ttggtttgtc
    18601 tgattcaggg attgacaggg ctgaaagcta cttcaaactt gaaaacctct atgacattga
    18661 aaacgtggct ttgactcact ttatcgataa cgcccttcgt gccaactaca tcatgcttct
    18721 cgatattgac tatgtggtga gcgaagagca agaaatcttg attgtcgacc aatttacagg
    18781 tcgtaccatg gaaggtcgtc gttattctga tggattgcac caagctattg aagccaaaga
    18841 aggtgtgcca atccaggatg aaaccaagac atctgcctca atcacgtacc aaaacctctt
    18901 ccgtatgtac aagaaattgt ctggtatgac gggtacaggt aagactgagg aagaagaatt
    18961 ccgtgaaatc tacaacattc gtgttattcc aatcccaaca aaccgtcctg ttcaacgtat
    19021 tgaccactca gaccttcttt atgcaagtat tgaatctaag tttaaagcgg ttgtcgaaga
    19081 cgttaaggct cgttaccaaa agggtcaacc tgtcttggtt ggtacagtag cggttgaaac
    19141 tagtgactac atttctaaga aattggtcgc agctggtgtt cctcacgaag tcttgaatgc
    19201 caaaaaccac tatagagaag cccaaatcat catgaatgct ggtcaacgtg gtgccgttac
    19261 catcgcaacc aacatggcgg gtcgtggtac cgacatcaag cttggtgaag gtgttcgtga
    19321 acttggagga ctttgtgtta ttggtacaga acgtcatgaa agtcgtcgta tcgataacca
    19381 gcttcgtgga cgttcaggtc gtcaaggaga tccaggtgag tcacaattct acctatctct
    19441 tgaagatgat ttgatgaaac gttttggttc tgaacgcttg aagggaatct ttgaacgctt
    19501 gaacatgtct gaagaggcca ttgagtctcg catgttgacg cgtcaggttg aagcagctca
    19561 gaaacgtgtc gaaggaaata actacgatac ccgtaaacaa gtccttcaat acgatgatgt
    19621 catgcgtgaa caacgtgaga ttatctatgc tcaacgttac gatgtcatca ctgcagatcg
    19681 tgacttggca cctgaaattc aggctatgat caaacgcacg attgaacgtg tcgttgatgg
    19741 tcatgcgcgt gccaaacaag atgaaaaact agaggcaatc ttgaactttg ctaagtacaa
    19801 cttgcttcct gaagattcta ttacgatgga agacttgtca ggcttgtctg ataaggccat
    19861 caaggaagag cttttccaac gtgccttgaa ggtttacgat agtcaggttt caaaactacg
    19921 cgatgaagaa gcagttaaag aattccaaaa agttttgatt ctacgagtgg tggataacaa
    19981 gtggacagat catatcgatg cccttgatca attgcgtaac gcggttggac ttcgtggcta
    20041 tgctcagaac aaccctgttg ttgagtatca ggcagaaggt ttccgtatgt ttaatgatat
    20101 gattggttcg attgagtttg atgtgacacg cttgatgatg aaagcacaaa ttcatgaaca
    20161 agaaagacca caggcagaac gtcatatcag tacaacagcg actcgcaata tcgctgctca
    20221 ccaagcaagt atgccagaag atttggattt gagccagatt ggacgcaatg aactttgccc
    20281 atgtggttct ggtaagaaat ttaaaaactg tcacggtaaa agacaataaa atgagatagt
    20341 ttagaggcgg atatcttgtg aaaagtaaat ttttactggg tatccgtttg ctttataagg
    20401 agatgagtta tggtatttac agcaaaaagc tctaaaataa atatagaaga agttcgtgcc
    20461 ttgtcaaaat tagaaggtca ggctttggag aggaaatcac agcgagatca agagctagaa
    20521 gccattatac gtggagaaga ccagcgaatt ctcttggtaa tcgggccatg ctcatctgac
    20581 aacgaagaag ctgtccttga atacgctagg cgtttggcag tcctacaaga agaagtggca
    20641 gatcgtatct ttatggttat gcgtgtttat actgccaaac cccgtaccaa cggagatggc
    20701 tataagggct tgattcacca gcctaacgcg acagaagcgc ctagtcttat caatggaatc
    20761 aaagccgttc gccatcttca ctatcgtgtc atcacagaaa cagggatgac aactgctgat
    20821 gaaatgcttt atcctgaaaa ccttccgctt gtagatgatt tgatttctta catggcagtt
    20881 ggtgcccgtt cagttgaaga ccagcaacac cgctttgtgg caagtggggc aggattttct
    20941 actggtttta aaaatccaac ctctggaaat ctcaatgtca tgtttaatgg gatttatgct
    21001 gctcaaaaca aacaaagttt ccttttctta ggaaaagaag tagaaacaac tgggaacccg
    21061 ctttcacacg ctattcttcg tggtgctctt aatgagtatg gaaaaaatat tcccaactac
    21121 tattatgaca atttaattga taccattgcc cagtatgaga aaatgggctt ggaaaatcct
    21181 tttatcatca ttgataccaa tcatgacaat tctggtaagc agtatattga acagatccga
    21241 attgtccgcc agaccttgat taaccgtgct tggaatgaaa aaattaagca gttcgttcgt
    21301 ggttttatga ttgagtctta tctggaagat ggtcgacaaa atgagccaga agtatttggc
    21361 aagtctatca cagacccttg cctgggttgg gataacacag aagctcttgt cagagaaatt
    21421 tacaaaacgt taggagaata agatggcatt tattgaaaaa ggtcaagaaa tcgatatgga
    21481 agtcatcaag gctgaaaccc aattgtctgc ggaagccttg agactcaagg aaagccgtga
    21541 cagggaattg gcagatatta tttcagggga agatgaccgt attctcttgg tgattggtcc
    21601 ttgctcttct gataatgaag aggcggtctt ggaatatgct cgccgtttat ccgccttgca
    21661 aaagaaggta gcggacaaga ttttcatggt catgcgcgtg tatactgcta agcctcgtac
    21721 caatggagac ggctataaag gattggttca ccagccagat acttctaagg ctccaagcct
    21781 gattaacggc ttgcaggctg tgcgccagtt gcactaccgc gtgattacag agactggttt
    21841 gacaacggca gatgagatgc tttatccgtc aaatctgatc ttggtggatg acttggtcag
    21901 ctaccatgcc gttggagctc gttctgtgga agaccaagag caccgctttg tggcttctgg
    21961 gattgatgca ccagtaggga tgaaaaatcc aacctcagga aatttgggtg ttatgtttaa
    22021 cgccatctat gctgctcaaa acaagcaaac cttcctttat catgggcagg aagttgagac
    22081 atcaggtaat cctttggccc atgttatcct ccgtggagca gtcaacgagt atggtaatta
    22141 tatgccgaat tactactatg aaaacctact ccaagccatt gaacgctatg aaaccatggg
    22201 acttgaaaat ccttttatcc tcattgacac caaccatgat aactcaggca agcaatatat
    22261 ggagcagatt cgaattgttc gccagacctt gcagaatcgt gattggaatg agaaaattaa
    22321 aaagacggtt cgaggattta tgattgaatc ttacctagca gatggtcgtc aaaaccaacc
    22381 agaggtcttt ggttgctcta ttactgaccc ttgcctaggt tgggaaaata cagaggcctt
    22441 ggtagaagag atttatgtta ccttgacaaa ataagtgaaa aggatggagt tggggaatct
    22501 caactccttt tgatgagaat gatagttgga cacggaattg acatcgaaga attggcttcg
    22561 atagaaagcg cagttacacg acatgaagga tttgctaagc gtgtactgac cgctcaggaa
    22621 atggagcgct tcaccagtct caaaggacgc aggcaaatag aatatttagc tggtcgctgg
    22681 tcggctaagg aggccttttc caaggctatg ggaacgggca ttagcaagct cggttttcag
    22741 gatttggaag tcttgaacaa tgaacgtggg gcgccttatt ttagtcaggc accattttca
    22801 ggaaagattt ggctgtctat cagccacacc gatcagtttg tgacagccag tgtcattttg
    22861 gaggaaaatc atgaaagcta gtccacatag accaaccaag gttctgattc atctgggagc
    22921 tattcgacaa aatattcagc aaatgggggc tcatatccct caaggaacgc tcaagtgggc
    22981 tgtggtcaag gccaatgctt atggtcatgg agctgttgcc gttgccaagg aaattcaaga
    23041 tgatgttgat ggcttttgcg tttccaatat cgatgaagcc attgaactca gacaagctgg
    23101 actcagcaag ccaatcctca ttttaggagt ttctgaaatc gaagctgttg ctctagctaa
    23161 agaatatgac ttcaccttga cagtggctgg actggagtgg attcaagcac tcttagataa
    23221 ggaagtggac ctaactggat tgacagtcca cctcaagatt gattcaggga tgggacggat
    23281 tggttttaga gaggccagtg aggttgagca ggctcaagat ttgctccaac aacacggtgt
    23341 ttgtgttgaa ggaatcttta cccactttgc tactgctgat gaggaatcag atgactattt
    23401 taatgcccag ttagaacggt ttaaaactat tttagctagt atgaaggaag ttccagagct
    23461 ggttcatgct agcaattctg caacgactct ttggcatgta gagactattt tcaatgcggt
    23521 tcgtatggga gatgccatgt atggtctcaa tccaagtgga gcggtcttgg atttgcctta
    23581 tgatttgata ccggccttga ccttggagtc tgctctggtt catgtcaaga cagttccagc
    23641 tggagcttgc atgggttatg gagcaactta tcaagcggat agcgagcaag tcatcgcgac
    23701 cgtgccaatc gggtatgcag atggatggac aagagacatg cagaatttct ctgtcttggt
    23761 agatggccaa gcttgcccaa ttgtcggcag ggtttcgatg gaccaaatca ctattcgatt
    23821 acctaagcct tacccactcg gaaccaaggt aaccttgatt ggctccaatg gggataagga
    23881 aatcactgca actcaggtag cgacctaccg cgtaaccatt aactatgagg tggtttgcct
    23941 cctcagcgac cgtattccga gagaatatta ttagaaaaga aaggagtgga gcatgaatct
    24001 acatcaaccc ttgcatgtct tacctggtgt gggactaaag tcagcagaaa aatacgccaa
    24061 actaggaatt gaaaacttgc aagatctctt gctctacttt cctttccgtt atgaagactt
    24121 caaaaccaag caggtgctgg agctggaaga cggtgagaag gcagttcttt ctggtcaggt
    24181 agtgactcct gctagtgtcc agtattatgg tttcaagcgc aatcgcctgc gttttagtct
    24241 caagcaggga gaggtcgttt ttgcggtgaa tttctttaac cagccctatc tggctgataa
    24301 aatagagttg ggagcaaccc ttgctgtctt tggaaaatgg gaccgcgcta aggctagtct
    24361 gactgggatg aaggttctgg ctcaggtaga agatgacctc cagcctgtct atcgtctggc
    24421 tcagggaatc agtcaggcca gtctggttaa ggtcatcaag acggcttttg atcagggact
    24481 ggacctcttg atagaagaaa atctgcccca gtctttacta gacaaataca aactcatgtc
    24541 ccgttgtcag gcagtccgag ctatgcattt tccaaaggat ttggcagaat acaagcaggc
    24601 tcttcgccgt atcaagtttg aggaactctt ttatttccaa atgcagttgc agacgctcaa
    24661 gtctgaaaat agagttcagg gaagtggtct ggttctgaat tggtctcagg aaaaagtgac
    24721 agcagttaaa gcaagtcttc cttttgccct gacccaagct caggaaaaga gtttgcagga
    24781 aattttaatt gatatgaagt ccgaccacca catgaatcgt ctcctacaag gagatgtggg
    24841 gagcggaaaa acggtagtcg ctggcttggc catgtttgcg gcagtgacag caggttatca
    24901 ggctgcccta atggtaccaa cagaaatcct cgcagagcaa cactttgaga gtttacagaa
    24961 cctttttccc aatttgaaac tggctctctt gacaggttcc ttgaaagctg cagaaaagag
    25021 agaagtcttg gagaccattg ccaagggtga ggctgatttg attatcggaa ctcacgctct
    25081 gatacaagat ggggtggagt atgctcgtct tggtttgatt attatcgatg agcagcaccg
    25141 ttttggtgtg gggcaaaggc gtattttacg ggaaaaaggc gacaatccag atgtcctcat
    25201 gatgacggcg actcccattc cacggacgct tgccatcaca gcctttggag atatggatgt
    25261 ttccattatc gaccagatgc cagcaggtcg gaaacctatt gtgacgcgct ggatcaaaca
    25321 tgagcaacta cctcaggtct tgacttggtt agagggggaa attcaaaaag gttcccaagc
    25381 ctatgtcatc tctcctttga ttgaagaatc agaagctcta gatttgaaaa atgccattgc
    25441 cttatcagaa gagctgacgg ctcattttgc aggtaaggca gaagtggctc ttctacatgg
    25501 taagatgaag agtgacgaaa aagaccagat catgcaggat ttcaaggaga gaaaaacaga
    25561 tattctggtt tcaacaacgg ttatcgaggt tggggtcaat gttccaaatg cgactgtcat
    25621 gatcatcatg gatgccgatc gcttcgggct tagtcagctt caccagctca gaggtcgtgt
    25681 cggtcggggg gacaagcagt cctacgctgt tctcgttgcc aatcccaaga cggattctgg
    25741 gaaagaccgc atgcgcatca tgacagaaac gaccaatgga tttgtccttg cggaggaaga
    25801 tttgaaaatg cgtggttctg gtgagatttt tggaaccaga cagtcaggac ttccagagtt
    25861 ccaagtggct gatattatcg aagattttcc gattttagaa gaagctagaa aagttgttag
    25921 ttacattagt tctattgaag gctggcaaga ggatccagag tggcgcatga ttgcctttca
    25981 tctggaaaag aaagaacatc tggattaagc tttctctaag gaaaacttaa gctagctttc
    26041 aggtttggcc cttatactag agtcatcaaa aagaaacgag gactctcata tgacagtaac
    26101 gattaaagta aattacccaa gcacttttca aaagaaagaa gcaacagatt aagattgagc
    26161 agaagaaaaa gggcggaaat gctctttttt cgtttctaaa actactttca gtcaataggc
    26221 ttgaaggaag aaaagctaaa ttcactttct atttaccctt ctttcttgca ttgctttctt
    26281 agatgtgcta cagtggtgct agcgattaca aaacaaaagg agcatgctat gaaaaatcca
    26341 gctttgttag aagaaatgaa gatctatcta ggaagggacg aggttccaga agacgttgat
    26401 gctttctggg atgaggaagt gaaaaaagtt tccacgcttc cagcctacca gttggaggaa
    26461 ataaatttcc acatttctca agtcaagtgc tatgagctaa cctttgaagg aagcaagcaa
    26521 ggcaaggtct atgcacgagt tgttcttcca aagagtgagg agaaggttcc gttaatcttc
    26581 catttccatg gttatatggg acgtggttgg gattgggccg acatgctggc tttcaccgta
    26641 gctggttacg gtgttgtttc catggatgtt cgaggccagt cgggctattc acaagacggt
    26701 ttgcgctctc ctcttggaaa taccgttaag ggacatatca tccgtggtgc tgtggaaggt
    26761 cgggaccacc tcttttataa ggatgtttat ctggacattt accagttggt tgaaattatt
    26821 gctagtctgc ctcaggtaga tgagaagcga ctttccagct atggtgcctc gcaaggaggg
    26881 gctctagctt tggttgcagc agcgctcaat cctcgaattc agaaaccagt tgccatctat
    26941 cccttcttgt cagacttcag acgagtgctt gagattggta atactagcga agcctatgac
    27001 gaacttttta gatatttcaa attttacgac cccttccatg aaactgagga ggagatcatg
    27061 gcgatccttg cccatcgtat caagggtgag gttaagatga ttacgggttt ggatgacgat
    27121 gtttgctatc ccattaccca gtttgcgatt tacaatcgtt tgacctgcga taaggcttat
    27181 cgcatcatgc ctgagtatgc ccatgaagcc atgaatgttt ttgtcaatga ccaggtttat
    27241 aactggctct gtgggagtga gattcctttc aaatatctaa aataaggagt cgactctagg
    27301 cacaaaatct taaaaatcac aaacacgcat agtatcaggg gattaagaag acttgatact
    27361 atgcgtttta tcatggaaat atagtaaaat gaaacaagaa caggataaat cgatcaggac
    27421 agtcaaatcg atttctaaca atgttttaga agtagatgtg tactattcta gtttcaatat
    27481 attatattta tagaattttt tgttgctaga tttgtcaaat tgcttaaaat aatttttttc
    27541 agaaagcaaa agccgatacc tatcgagtag ggtagttctt gctatcgtca ggcttgtctg
    27601 taggtgttaa tacttttcaa aaatctcttc aaaccacgtc agcttcgtct tgccgtaggt
    27661 atggttactg acttcgtcag ttctatccac aacctcaaaa cagtgttttg agcatcatgc
    27721 ggctagcttc ctagcttggt ctttgatttt cattgagtgt aaaaaaacag atgagtttct
    27781 gttttctttt tatgaactat aaatgttcag ctgaaactac tttcaaggac attattatat
    27841 aaaagagttt tttgaaacta aaatctacta tactacacta tattgaaagc gttttaaaaa
    27901 tgaggtataa taaatttact aacacttata aaaagtgata gaatctatct ttatgtatat
    27961 ttaaagatag attgctgtaa aaatagtagt agctatgcga aataacagat agagagaagg
    28021 gattgaagct tagaaaaggg gaataatatg atatttaagg cattcaagac aaaaaagcag
    28081 agaaaaagac aagttgaact acttttgaca gtttttttcg acagttttct gattgattta
    28141 tttcttcact tatttgggat tgtccccttt aagctggata agattctgat tgtgagcttg
    28201 attatatttc ccattatttc tacaagtatt tatgcttatg aaaagctatt tgaaaaagtg
    28261 ttcgataagg attgagcagg aagtatggtg taaatagcat aagctgatgt ccatcatttg
    28321 cttataaaga gatattttag tttaattgca gcggtgtcct ggtagataaa ctagattggc
    28381 aggagtctga ttggagaaag taaagggact ccaaaatgca actgttcatg tggagttcaa
    28441 accggctgct gatggtccta gtttttacaa tctcttttct gcttccagta caactaaagt
    28501 aaatgaatac tttacaatgg caatcaataa tgggacagct ttgatagagg gacgtggagc
    28561 tgatggtagc caattttatg gaagttatac agatgcgcct ttgaagatta gaccaggcaa
    28621 gtataattcg gttactttta ctgttgaaag accaagaaag gatagtccaa atggtcaggt
    28681 tcgtctttat gtgaatggtg tattatctcg tacgaataaa aagtcaggga aattcctggc
    28741 agatatgcca gatgtagata aactccagtt aggtgcaact aatagagcag gagaactgaa
    28801 gtggagctca gatctttcta ttcgtaatct gactgtatac aatcgtgctc taactccaga
    28861 ggaagtcaaa aaacgtagcc agttgtttga tgtgatagat attgagcctt tacttgctga
    28921 aggggcagtc ttgacagaga agcaagagtt gtttatgagt gatgtcaatg gtaagccaaa
    28981 tagtgaggga attaagagtt atcggattcc agctttgcta cgtacggata aaggaacatt
    29041 actggcaggg gcagatcagc gtcgtctcca ccattctgat tggggagatg ttgctatggt
    29101 cgttaggaga agtgaggatg ggggaactac ttggcagcca accttaacct tgaccaacct
    29161 gcgagacaat ccagaagcaa aagatccgca ggcatcatct ccacttaata tcgatatggt
    29221 cttggttcaa gatcctacca caaagagaat tttttcaatc tacgatatgt ttccagaggg
    29281 tcgagctgtc tttggaatgc caaacaaacc tcaaaaagct tatcaacaag ttggagacaa
    29341 gcactatcaa ttactatata aacaagggga aaatcaagca tatactgttc gagaaaacga
    29401 agaagtatat gatgcaaata atcaaaaaac agattatcgc gttgtagtgg atccaaaaga
    29461 agaagcctat agagataagg gcgacctcta taaaggagaa gagcttcttg ggaatatcta
    29521 ctttgctcaa tctgctaaaa ctccatttcg tgtagcctat acgagctatt tgtggctttc
    29581 ctatagtgat gatgatggga aagcttggtc gcaaccaaga gatattacac catcaattcg
    29641 ccaagattgg atgaaatttt taggaacagg tccaggtaca ggaattgtgc ttagaacagg
    29701 agagcacaag ggacgtatcc tagttcccac ttataccacc aatgctatct cccatctaag
    29761 cggctcccag tcttcacgtt tgatttattc agatgatcat ggagaaacat ggcaggctgg
    29821 agctgctgtt aatgatgata ggacggtagg cagaaggaaa attcattcct caactatgaa
    29881 taataggaat gcccaaaata ctgagtcagt tgctgtgcag ttaaataatg gtgatgtgaa
    29941 actctttatg agagggttaa cgggtgattt acaggttgcc acaagtaaag atggtgggca
    30001 aacttgggac aaggaaatca agagatataa tcaggttaaa gatgtttatg tccaaatggc
    30061 tgctattcac accatgcacg aaggaaaaga atatatcatt ttaaccaatt caggaggacc
    30121 taaacggacg aatggaatgg ctcatttggc tcgtgtagag gacaacggag acttgacttg
    30181 ggttacatca tagaccaatt caaaaaggag agtttgccta taattcgctt caagaattag
    30241 gaaatgggga gtatggtatc ttgtatgaac atactgaaaa aggacaaaat gactataccc
    30301 tatcatttag aaaatttaat tgggactttt taacaaagga gccggtatat ccaacgagtg
    30361 taactatcag ggacgttcgt aaattggaaa cagaagaaga ggatgcagaa caaggcatct
    30421 tagctatgca atttgattct gaggtactag tgaatgctat tccgactttg actttagcga
    30481 atggacacaa agctaccttc ttgacccaag cagatcaaaa aactctactt ttcaccttta
    30541 ataaagaaga tgcaggtcaa gaaattacag gtctaatggc cggtagaatt gacagtatgc
    30601 atgatttacc agttacacta gctggtagta gaattcctga agatgcgaaa gaaaatcctg
    30661 tcgagaccat gaatacagta agagaaaatg tatctgagga gatgacagaa aggaagtcag
    30721 agaaggataa attatctttg gagtcttcag atagaatggt agcaaactct cctcttactt
    30781 cttttgctcc tcgttacctc caatcttatg taggagatgt tattaaaact gagactaaag
    30841 ttccaataac gactggttgg aagcaagaaa atggtgcgtg gtatttttat acatctgctg
    30901 gtgaagtggt gaaaggctgg catcaggaag cggataaatg gtactacttg agttctactg
    30961 gtgcgatggc aactggttgg gtcagagatg gtaatcaatg gtattatttg agtgagagtg
    31021 gagcaatgtc tactggctgg gttgaatcca gtggtgtgtg gtactatctc cattctaacg
    31081 gttcaatggc gactggttgg ataaaagatg gagaccattg gtactatcag gaatcatctg
    31141 gtgcaatgag ggtaaatcaa tggttccaag ttggagacaa atggtactat gtcaatgaaa
    31201 gcggaagatt agctgttaat accatagtgg atggttatca agttaactcc aatggagagt
    31261 gggtcaacta ctagagactt ggatgttgat gaataactga gacacgtttt attcgtcata
    31321 gagattaaaa gtttatttag atagttttcg tagagatttc tactctaagg aaaaatcaat
    31381 ttaaatgagc ttctattgcc gggtaaatga cttgctaatc gtaaataaac tgtatatttc
    31441 cgacttactt ccgactggat actactagtc gcaatttaac agttcgcttt aagtgttaaa
    31501 atgcatatag ctagttattg gttatatgcg tttttgttct tgattatttt atttcttgtc
    31561 ttggaatata ggtaaaatta tagtaaaata aaattctgta attcatgttg gtagatatac
    31621 aggaagaaga gtgtattgtt gacaatatag cacatagaat ccaatatgtc cttatactca
    31681 aaaaatcaaa gagcaaatta ggaagctagc cgtagcctgc ccaaaacacc gttttgaggt
    31741 tgtagatgaa actgacgaag tcagctcaaa acaccgtttt gaggttgcag atagaactga
    31801 cgaagtcagt aacatataca tacggcaaga cgaagatgac atggtttgaa gagtatggaa
    31861 caatcataac ttatttgaag attttaaaag attataagct attatatctg gataaataag
    31921 tattagaaac taaagtgagg aaaagatgtt tttatattta aaaggtttag gtaaatatcg
    31981 taagtcaaaa gcttacgggt tggtgggatg tctggcatta gtgagtatga ttggattagc
    32041 tgcaccagac ttaccagtta ttggtggtgg agtcgttgct gctgatgtta ttcagggtgg
    32101 taacgatata aaagatgtga acgttcatag taaatctgcg gaaggtgttg ctatgaccta
    32161 taccacttat gatagcggaa caagtggaaa acaaaccgca tcaggtagcg gtgtctttgt
    32221 agcgccgaat gtgatggtaa cagtagctca taactactat gataaaaacc aagaggataa
    32281 gtctgcggtc ttgcgtggtg gggcgtctgc tcgtagttat gttgtgatga actcagagac
    32341 ggaaaagcac aataaagtac ctacttctgg tgtatcagaa actcttgaaa aagactctat
    32401 tcatttgtat gatgagaaaa attttgggaa agactatagc aacgacttag cagtagtggt
    32461 aactaaaaag actgtagaag ctatgacagg tggtgaagat tcaccaagag aattgagcca
    32521 taaagaggtt tctactggtg ataaaatctc tatggtcggc tatcctaatg acttttcaac
    32581 tccgaattta agtgcagaaa acaaagcacg attgaaagac ggtaaggctt attcagttac
    32641 aacaactgta agtagtgtca ataaagagag tggtacagtc acttatcatt cctcagcttt
    32701 aggaggtttt tcaggtgctc ctttgtttaa tgataaggga gaggtagtcg gtatccacca
    32761 acatgggaca aatactccaa acgctcaaga aagtgagcgt attggtggtg gtaccctctt
    32821 tacggaaaag cacagagctt ggattcgttc tatgattgat aaatatggta taaagggttg
    32881 gtatatagat ggtgcaaacc gttactacta tgatgaaaat cacagagcct taaaagatgt
    32941 agagtctgag attgacggtg ctttgtatcg ttttgatgaa aaaggccgag ctactttact
    33001 agagggtgaa gaaaaaggtc gcgttctact tcgagtggaa gatactaaag gaactccttt
    33061 gatttcagat aaggttgttc aagaaggttc tgttggaagt ggtttgaatt ttcatttaag
    33121 acaaaatcca aacttcaaac agttaatagc aacttctcca acagctaaag tggtatccta
    33181 taacggagtg ccaattaaca aattagcaag tgatacaagt tggtctgatg aatatgtcag
    33241 taagttagct ttgggtgata caattataag agcggtagta gattcggtaa ctcctccatc
    33301 tacgtcttct tcggattttg caagaactga agttggtaag gttgatttga gtggtaaatc
    33361 gaacttacct gtgcctagta aagaggtatt acaagctccg aatggttcag aaaacttcta
    33421 tgctacaacg catattcaaa cgccagatgg gtcggggtca ggtactttaa ttgcaccaaa
    33481 tttggtgtta acagtcgctc ataatttctt aacagttaaa ggttctgagg tagttacgaa
    33541 gtctggtcgc accaatacag tgtataaagc tactttgcca agtggtcagc ctgtgaattt
    33601 ttccgatgat gatattgttt attggaacaa gaaagactca gtatttggat ttaaaaatga
    33661 cttagctttg gttcgtttga aagaaaagct tacagcggta tctcctgtag aggtggtatc
    33721 tgagtcaact tcgattacta aaggtgacaa agtttccgtt tatggttttc ctgatggtcg
    33781 tttgtctcca gttttggata gtgaagtagt agctactact gactttggtt caggtattga
    33841 agggattagc tatggtggta caaaacccgg agcttctggt ggtggtcttt ataatgacaa
    33901 aggttcttta attggagttc accaaaatgg tgttgtagga agccgcagtg gtgggttagt
    33961 cttatcaaaa gaacaattag attgggttcg ttcctatatt gagggtaaac ccaaagcccc
    34021 tgtttatgta acagataata ttttggtgga tgaaaaagac aaggataaac ttccatcaac
    34081 ttcaaaagaa gaaaaaccga ccacaccaaa agtagagtcg gataaagata aaccaaatac
    34141 acctctaaaa ccgcaagaaa agccgaaaac agaggttata acgagttatg agggtgatag
    34201 tacccttgaa gttgggaaag agcgtacaga ggaaactgag ggcgaaaaag aaggtgtttc
    34261 ccttatttat cgaacggtgt ataaaggtac taaatcgaaa acagaaatgt cacctattgc
    34321 ctttgacacg gtttaccaag gagatgaaac taaagagctt ggtttccgtt cagttttaga
    34381 gggtaaagag ggtctagtta ctcgcactac aagttaccaa gtagataagt acacaggagc
    34441 ggtatcctcc aagatttctg aagagaaaat agcacctcaa tctcaagtca tcacattagg
    34501 tattaagaaa aatagcagca caaaagaagt tccaattaca gaacgttttg aagattccgc
    34561 agaactagag aaaggtaaaa ctgaggttat ttctgaaggt tctgtaggta aagaggttac
    34621 tacggttact tataaggttt tacctgatgg aaaggttatt gaaaattctc gtacagttga
    34681 cgttacaccc atgagagagc gtgtagttcg taagggtgtg aaggaagtgg tatctccaga
    34741 taaagtagag tctctagttc caaaagatgc gccgattaga gaagagcagc ctgcgcttag
    34801 tgagggattt tcagagtcag atgctttagt atcaggagaa aaaatacaag gagatcttgg
    34861 gatacttata gtatcttcag aagaactagt ccctgaaaga gtagaagttc cagattttgt
    34921 gactaaagtt acaggtggag aaaaattgac agtagaaggg caccgaaatg agagtaaaat
    34981 aaagactcca tcaaaacagg aaagatcatc tcgcccagaa accactgctc aattcacaac
    35041 gaacgggaca ggttcgtcat cgttaacagc tgtttttggc ggtaaaacgg ataaaatatt
    35101 actttctact gttgaacatt ctgttattaa acataatcaa caaagaggat ggcataagat
    35161 aaataatcag tggtatttta gaaattctga tgggaaagaa cggacaggtt ggatgaaaga
    35221 aaatgatgca tggtattatt ttgatacgaa tggaaccatg caaactggtt ggctagagga
    35281 tacagacggt aattggtatt atctcaatga taatggtagg atggagatag gctggttcca
    35341 agattcaagt ggcgcgtggt actacttagg atcatctggt cgcatggaat ccaatacatg
    35401 gatttattat aaaggaaagt ggtactatat tgatgctttg ggtaaactac ttttcaattc
    35461 ggtaacacca gacggctata gagtgaacga gtatggggaa tggatcaact gaacttgctt
    35521 tatggcatat tgacgagaaa gctaggagta taaaaacact agaattcata gtctagaaag
    35581 tggtaaggct tttctaaaat ggttgatttt gtaatctttt ttgtaaaata tagcttttaa
    35641 taaataatgt gagcaagata tatctctttg caagtgatta gggtcaatag tttttaactt
    35701 tctcacttct tttccgaata aatagataga accatagaat ctagcaaaat tagatttaaa
    35761 actatggtat aatagaggga ggagaaggat aattctcaga catccgggca tcagcccaac
    35821 caatgactta gttgctaaga aaatctttag caatccggaa atcacttgtc aatttattcg
    35881 cgatatgctg gacttgccag caaaaaatgt gactattttg gagggaagta acattcatgt
    35941 tttgccttct atgccttact cagctcaaga tttttacact agtatagacg tcttggcgga
    36001 gttggataat ggtactcaag taatcattga gattcaggtg catcatcaga attttttcat
    36061 caatcgcttg tgga
//
LOCUS       DAVKDZ010000012        35831 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000012 DAVKDZ010000000
VERSION     DAVKDZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35831
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.012"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            34..5250
                     /locus_tag="VWZ30_000596"
     CDS             34..5250
                     /locus_tag="VWZ30_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9334391.1"
                     /translation="MEKYFGEKQQRFSFRKLSVGLVSATISSLFFMSVLGSSSVEAQE
                     TQETKGVHYKYVTESELSSDEKKQLVYDIPTCMENDDETYYLIYKLNSQNQLGELPNT
                     GSKNDMKTLVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSAHALENN
                     LLLNYNTDYELTTGEKLPLPKEISGYTYIGYIKERNITSESKVNNQEKSVDTSKNQQK
                     VDYTIAPNFIESPSKIQVINGQYETVPEFRDGQVGAVATESAATKADTLSSDMNSEVE
                     SHSIPPTVDSSQNGENLGKQGEDATPVEKPKEQEPEKTEEVPVNPTEGTTEGTSNQGA
                     KNPVQPAEESTTNSEKISPDTSNENTGEVSSNPSDSKPPVEETNKPEKDESKDETSTK
                     PENSAHPITESGQTEPDQPKGNSPEDVSTESKTSNSNESEEIKHKTEVEPDKKVEEQE
                     KTIELRSVSDVELYSYEGNQSKQHTSLDKVPDNKSAYFIKVKSAKFKDVLLPVSNIED
                     TTKDGKAVYKITARLEHLKQSVDNQYQDNFTYYLPKLVQNDSPIYTSFKKLITDMNSR
                     PSGTFTLGATMNAREFELNENQESYVTSKFTGTLIGSHQGKHYAIYNLKKPLFNTLNN
                     ATIQNLTLKDVNISGKNHVASVAKEATNSSTLDNVHANGIIAGELGIGGLVQKVDNST
                     ISNSSFTGRITNTFVTTSQYAIGGLVGQLTGTNASVDRSIATVTISSNAEYSNQTIGG
                     IAGIVENNATVQNSYAEGEINNVKPFARVGGVAGSLWTNGSSTEDNSGILQNVLSDMN
                     ITNGNAISGYHFNGMKKKAVYSNKDNKVVNVQAVGDEVMSIDSTVERGTEIEKTAVQA
                     KKEQITKKHTITVEDFTNSVKQDVDYTKATHYQVARDIAYKNIEKLLPFYNRDTIVKY
                     GNQLSTDHNLFKKELLSVVAMKNEEVVSDITSNKNSINHLLLHYKDGTSEKVNVTYHS
                     DFANLAEYTIGTTGLVYTPNAFLKDYTSIIDRVKNDLNTVQYDPTSLKNLLGISDNVK
                     LTELYLDEQFAKTKEHLTETLKKLLSADAAVSGNNDIIDNYIVDKIKRNKEALMLGLT
                     YLERWYDFKFDKASAKDLLMFHMDFFGKGNTSPLDTIIELGKSGYNNLLAKNNVVTYN
                     ALLTNNYGTKDLFSALEGYRKAFAPTQTNNDWFKSQTKAYIVEEKSNILEVKANQEKA
                     GSKYSIGVYDRITSDSWKYRNMVLPLLTLPEKSVFVISTISSLGFGAYDRYRNKEHQA
                     SGDLNSFVEENARETAKRQRDHYDYWYRILDEKEREKLYRNILLYDAYKFGDDHTEGK
                     AKKVATFDDPNPAMQHFFGPVGNKVGHNEHGAYATGDAVYYMGYRMLDKDGAITYTHE
                     MTHDSDQDIYLGGYGRRSGLGPEFFAKGLLQAPDHPNDATITINSILKHSKSDSTEGQ
                     RLQVLDPTTRFNNADDLKQYVHNMFDVIYMLEYLEGKSIISQLSATEKMTALRKIENK
                     YVKDREDGNEVYATNVVQNLTEEDAKKLTSFENLIDNNILSAREYKSKEYERNGYFTI
                     KLFAPIYAALSSDIGTPGDLMGRRIAYELLAAKGFKDGMVPYISNQYEEVAKQNGKKI
                     TIYGKERGLVTDELVLQKVFNGQYETWTEFKKAMYNERVAQFDRLNKVTFNDTTQPWQ
                     TFAKKTTSSVDELQKLMDVAVRKDAEHNYYHWNNYNPDIDSEVHKLKKAIFKAYLDQT
                     DDFRSSIFENKK"
     gene            5299..10413
                     /locus_tag="VWZ30_000597"
                     /pseudo
     CDS             5299..10413
                     /locus_tag="VWZ30_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473008.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
     gene            10673..11524
                     /gene="ylqF"
                     /locus_tag="VWZ30_000598"
     CDS             10673..11524
                     /gene="ylqF"
                     /locus_tag="VWZ30_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEU9334392.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTCALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            11511..12290
                     /locus_tag="VWZ30_000599"
     CDS             11511..12290
                     /locus_tag="VWZ30_000599"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU9334393.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            12306..13856
                     /locus_tag="VWZ30_000600"
     CDS             12306..13856
                     /locus_tag="VWZ30_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU9334394.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQILGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            14440..15510
                     /gene="xerS"
                     /locus_tag="VWZ30_000601"
     CDS             14440..15510
                     /gene="xerS"
                     /locus_tag="VWZ30_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEU9334395.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDLVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
     gene            16211..17488
                     /gene="ltrA"
                     /locus_tag="VWZ30_000602"
                     /pseudo
     CDS             16211..17488
                     /gene="ltrA"
                     /locus_tag="VWZ30_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(17554..17910)
                     /locus_tag="VWZ30_000603"
     CDS             complement(17554..17910)
                     /locus_tag="VWZ30_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEU9334396.1"
                     /translation="MMKYIFSEEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFT
                     SGKVEVFANVTESKIQDIKIYGDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITR
                     YFAGISREEIAEAVVG"
     gene            complement(17978..19663)
                     /gene="lpdA"
                     /locus_tag="VWZ30_000604"
     CDS             complement(17978..19663)
                     /gene="lpdA"
                     /locus_tag="VWZ30_000604"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU9334397.1"
                     /translation="MPKVGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPVMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIATKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            complement(19727..20770)
                     /locus_tag="VWZ30_000605"
     CDS             complement(19727..20770)
                     /locus_tag="VWZ30_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEU9334398.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYSEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            complement(20987..21954)
                     /locus_tag="VWZ30_000606"
                     /pseudo
     CDS             complement(20987..21954)
                     /locus_tag="VWZ30_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
     gene            complement(21970..22938)
                     /locus_tag="VWZ30_000607"
     CDS             complement(21970..22938)
                     /locus_tag="VWZ30_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU9334399.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRCMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGTMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESVFEDIYAD"
     gene            complement(23092..24453)
                     /gene="pdrM"
                     /locus_tag="VWZ30_000608"
     CDS             complement(23092..24453)
                     /gene="pdrM"
                     /locus_tag="VWZ30_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEU9334400.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            complement(24464..24724)
                     /locus_tag="VWZ30_000609"
     CDS             complement(24464..24724)
                     /locus_tag="VWZ30_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334401.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPASNRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            complement(24738..26006)
                     /locus_tag="VWZ30_000610"
     CDS             complement(24738..26006)
                     /locus_tag="VWZ30_000610"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU9334402.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DASGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQEVLQSAAKDKINVKTVATITKNFNGQDLTDFKALLEVGAVGFSDDGIPLESSKVV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAKEHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAQVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPSKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            complement(26019..26483)
                     /locus_tag="VWZ30_000611"
     CDS             complement(26019..26483)
                     /locus_tag="VWZ30_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9334403.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            complement(26493..27146)
                     /locus_tag="VWZ30_000612"
     CDS             complement(26493..27146)
                     /locus_tag="VWZ30_000612"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU9334404.1"
                     /translation="MEHSSWHALIKEQLPKGYFGKINQFMEQVYAQGTIYPPKEKVFQ
                     ALLTTPLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAVQANGHAGQIWEPFTDAVIQVVNQLDKPVVFILWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANALLKETGQEPIDWLR"
     gene            complement(27192..27641)
                     /locus_tag="VWZ30_000613"
     CDS             complement(27192..27641)
                     /locus_tag="VWZ30_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334405.1"
                     /translation="MTTKKANITIEEYIEMSEVDFNETVNYEFTSDTCQLANSIYQSL
                     FKFFDKKNFSGDLIFTWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLN
                     MNRNGCMGDFPHDFFDIYLDHVAKYAYEQKVNNIKEYYPLNCKKWFR"
     gene            complement(27655..28368)
                     /locus_tag="VWZ30_000614"
     CDS             complement(27655..28368)
                     /locus_tag="VWZ30_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU9334406.1"
                     /translation="MSYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQGKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIDRDYDLYAATNGITAIQTGRLAQSGLEPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVH
                     SYQDLLDCLDKNILEKITF"
     gene            complement(28816..29190)
                     /locus_tag="VWZ30_000615"
     CDS             complement(28816..29190)
                     /locus_tag="VWZ30_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEU9334407.1"
                     /translation="MESIGLVIVSHSKNIAEGLVELISEVAKDVPITYVGGTEDGGIG
                     TSFDQVDRVVSENPADTLLAFFDLGSAKMNLEMVADFSDKSIMINRVPIVEGAYTAAA
                     LLQAGAELSVIQTQLSELEINK"
     gene            complement(29190..29765)
                     /gene="dhaL"
                     /locus_tag="VWZ30_000616"
     CDS             complement(29190..29765)
                     /gene="dhaL"
                     /locus_tag="VWZ30_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEU9334408.1"
                     /translation="MNAEQALEWMKLFNEKIQEQKDYLSELDTPIGDGDHGGNMARGM
                     AAVMENLEGKQFETSSDVFKVVSMQLLSKVGGASGPLYGSAFMGMSKADSHSKLEEIL
                     QSGLDMIVKREKAVVGEKTMVDVWTPAIAALSDGQLTHELIDKVVNATKDLLATKGRA
                     SYVGERSLGHIDPGSFSSGLLFTAILETGVV"
     gene            complement(29787..30776)
                     /gene="dhaK"
                     /locus_tag="VWZ30_000617"
     CDS             complement(29787..30776)
                     /gene="dhaK"
                     /locus_tag="VWZ30_000617"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEU9334409.1"
                     /translation="MKKIINKPTQVVDEMLQGLSFMHDDLVERLDGFDVIVRKVEKTG
                     KVGLISGGGSGHEPSHAGFVGDGMLSAAICGAVFTSPTPDQILEAIKAADEGAGVFMV
                     IKNYSGDIMNFEIAQELAEMEGIDVASVVVDDDIAVENSLYTQGRRGVAGTILVHKIL
                     GAAARSGKSLSEMKDLADKLVLEIKTIGLALSGATVPEVGKPGFILEDDEFEYGVGIH
                     GEPGYKKEKMQPSAQLASELVEKLSEGFQLKAGERYGLLINGLGSTPLMEQYVFANDV
                     AKLLHEKGVELSFKKIGNYMTSIDMAGLSLTLIRLANDEWLDALNAPVTTPAW"
     gene            30930..31460
                     /gene="dhaS"
                     /locus_tag="VWZ30_000618"
     CDS             30930..31460
                     /gene="dhaS"
                     /locus_tag="VWZ30_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEU9334410.1"
                     /translation="MASSLITKKRIAKAFRDLLATREFDKISIVDIMESAGIRRQTFY
                     NHFLDKYELLDWIFETDLTEYITNNLDFISGQKLLQELFLYFEQERDFYIQLFDIQGQ
                     NNFYDQFISYCRLLVSKILREYGQIDIDSSAYTCFLLDYHSHALAEIVKAYVNKKSPV
                     PQPDFLIMTIIGKRPQ"
     gene            31457..32446
                     /gene="dhaQ"
                     /locus_tag="VWZ30_000619"
     CDS             31457..32446
                     /gene="dhaQ"
                     /locus_tag="VWZ30_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DhaKLM operon coactivator DhaQ"
                     /protein_id="HEU9334411.1"
                     /translation="MTFISNHKQKIISSYISATVSSHPELEKHPTLPLVYQKNRNPHQ
                     VPILSGGGSGHEPAHIGYVGEGMLTAAIYGQLFTPPTRTEILESIRFLNNGHGVFIIV
                     KNFEADIKEFSWAINTARQEGIKVGYSLAHDDISIEPHNRFQIRGRGLAGTILLHKVL
                     GYAAQNGADIEQLTDLGHELAPEIATIGFATKSASLPQATLPLFNLEEGNISYGIGIH
                     GEEGYRIVPFQSSEILANEIISKLRLHYHWKKGDQFILLVNNLGTTSNLEMGIFINDI
                     LQLLEIEGVTITFIKSGTFMTSLDMAGISVTLCPVKNKQWLKALNAPTTAFAW"
     gene            complement(32508..33080)
                     /locus_tag="VWZ30_000620"
     CDS             complement(32508..33080)
                     /locus_tag="VWZ30_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEU9334412.1"
                     /translation="MPRPKTKEELVLASKENYDKLNRFISQLSEDELQTPFDFSKDQK
                     KKEAHWKRDKNLRDVLIHLYEWQQLLLTWVHSNQKGHERPFLPEPYNWKTYGKMNVTI
                     WKKHQKTSLEEATRLLEQSHREVLELMEGFSNDELFAKGIYKWTGGTSLGSYFVSSTS
                     SHYDWALKKIKAHQKNCKMQMDSLKKNEFF"
     gene            complement(33138..33632)
                     /locus_tag="VWZ30_000621"
     CDS             complement(33138..33632)
                     /locus_tag="VWZ30_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEU9334413.1"
                     /translation="MSKEIDIEYYHQLALQKQKEHRKVLANLKKKPPKNLDKIAQQIH
                     QEVFEEIDCTACANCCKTLGPDFKEADITRIAKYFKMKLPAFEAEFLQVDEDGDKVFK
                     SMPCPFLGGDNLCSIYDVRPKACREFPHTDRKKIHQINHLTIKNTLTCPAAYLFVEKL
                     KDKL"
     gene            complement(33767..35500)
                     /gene="ptsP"
                     /locus_tag="VWZ30_000622"
     CDS             complement(33767..35500)
                     /gene="ptsP"
                     /locus_tag="VWZ30_000622"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU9334414.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFNEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(35506..35769)
                     /locus_tag="VWZ30_000623"
     CDS             complement(35506..35769)
                     /locus_tag="VWZ30_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEU9334415.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADAGDAIAAISETMEKEGLA"
BASE COUNT        11402 a         7070 c         6823 g        10536 t
ORIGIN      
        1 taacatctat aaattgtatt tcaaggagga atcatggaaa agtattttgg tgaaaaacag
       61 cagcgttttt catttagaaa attatcagta ggacttgtat ctgcaacgat ttcaagttta
      121 ttttttatgt ctgtattagg tagttcatct gtagaggctc aagagactca agagactaaa
      181 ggtgttcact ataaatatgt gacagagtca gagctatcat cagatgaaaa gaagcagctt
      241 gtctatgata ttccgacatg catggagaat gatgatgaga cttattatct tatttataaa
      301 ttaaattctc aaaatcaact gggagaattg ccaaatactg gaagcaagaa tgatatgaaa
      361 accttagttg ctggtgctag cttagctgct ctggggattt taatttttgc cgtttctaag
      421 aaaaaagtta agaataaaac agtattacat ttagtattgg ttgcagggat aggaaatggt
      481 gttcttgtat cggctcatgc tttagaaaat aatctcttac tgaattacaa tactgactat
      541 gaattgacca caggagaaaa attacctctt cctaaagaga tttcaggcta cacttatatt
      601 ggatatatca aagagagaaa cataacttct gagtctaaag taaataatca agaaaaatca
      661 gtagatactt ctaaaaatca acaaaaggtg gattatacga ttgcaccgaa ttttatagag
      721 agtccatcaa aaatacaagt gattaacgga cagtatgaaa ctgttccaga atttagggat
      781 ggacaagttg gagctgtagc tacagagtcc gctgcaacta aagctgatac attatctagt
      841 gatatgaact cagaagtgga aagtcactct atacctccaa ccgtagattc ttctcaaaac
      901 ggagagaatc ttggaaaaca aggagaagat gcaactccag tcgagaagcc gaaagaacaa
      961 gaaccagaaa aaactgaaga agttccagta aatccaacag aaggcactac agaaggaacc
     1021 tcaaatcaag gagcaaaaaa tccagttcaa cctgcagaag aatcaacaac gaattcagag
     1081 aaaatatcac cagatacatc taacgaaaat actggggaag tgtccagtaa tcctagtgat
     1141 tcaaaaccac cagttgaaga aacaaataaa cctgaaaaag atgagtcaaa agacgaaact
     1201 tcaacaaaac ctgaaaattc tgctcatcca ataacagaga gcggacaaac agaaccagac
     1261 caaccaaaag gaaattcgcc agaggatgta tcaactgaat caaaaacatc caattcaaat
     1321 gaaagcgaag aaattaaaca taaaactgaa gtagaacctg ataaaaaagt agaagaacaa
     1381 gagaaaacaa tagaactaag aagtgtttct gatgtggaac tctatagtta tgaaggcaat
     1441 caatcaaaac aacatacaag cttagataaa gtaccagata ataaatctgc ttatttcatc
     1501 aaagtgaagt cagctaaatt taaagatgta ctgctcccag tttcaaacat tgaagacact
     1561 acaaaagacg gtaaagcagt ctataaaatt acagctagat tggaacactt gaagcaatct
     1621 gtagacaatc aataccaaga caattttact tattatcttc caaaacttgt tcaaaacgat
     1681 agccctatct acacatcatt taaaaaatta ataactgata tgaatagtag accaagtgga
     1741 acattcacac ttggtgcaac aatgaatgct cgcgagtttg agttaaatga aaaccaagaa
     1801 agttatgtta caagtaaatt taccggaaca ttaattggta gtcatcaagg taaacattat
     1861 gctatctata atttgaaaaa gccgttattt aatacattaa ataatgctac tattcagaat
     1921 ctcaccttga aagatgtcaa tatttctggt aagaatcatg tcgcatcagt tgctaaggaa
     1981 gccacaaatt cttcaacttt agataacgtt cacgcaaatg gaatcattgc tggtgagcta
     2041 ggtatcggag gacttgtcca aaaagttgat aattctacca taagtaatag tagcttcact
     2101 ggaagaataa caaatacctt cgttacaacc tctcagtatg caattggtgg tctagtcggt
     2161 caattaactg gtacgaatgc tagtgtagat cgttctattg caacagtaac catttcaagt
     2221 aatgcagaat actcaaatca aaccattggt ggaattgcag gtatcgtaga aaataatgcg
     2281 actgtacaaa atagctatgc tgaaggtgaa ataaataatg ttaaaccatt tgctagagtt
     2341 ggtggagttg caggttccct ttggacaaat ggctctagca cggaggacaa ttctgggatt
     2401 ctacaaaatg tcctaagcga tatgaatatt acaaatggga atgcgatttc tggttaccac
     2461 ttcaatggta tgaagaaaaa agcagtttac agtaataaag acaataaagt agtcaatgtt
     2521 caagcagttg gagatgaggt aatgtcaatt gattccactg tagaacgtgg tactgagatt
     2581 gaaaaaacag cagttcaagc taagaaagag cagattacta aaaaacacac tataaccgta
     2641 gaagatttca caaattctgt taaacaggat gtagattata caaaggcaac tcattatcaa
     2701 gtggcaagag atattgcata taaaaatata gaaaaattat tgcctttcta taatagagat
     2761 accattgtga aatatggaaa tcaattatct actgatcata acctgtttaa aaaagaattg
     2821 ttgtctgttg tagctatgaa aaatgaggaa gtagtctctg atatcacttc aaataagaac
     2881 tctatcaatc acttactact acattacaaa gacggaacat ctgagaaagt aaacgtaacc
     2941 taccatagtg actttgcaaa tctggctgag tatactattg gaactacagg tctagtatac
     3001 acaccaaatg ctttcctaaa agattataca tctataatag acagagttaa aaatgattta
     3061 aatacagttc agtatgatcc aacttctctt aagaatttgt taggaatctc tgataatgtt
     3121 aaattaacag aactctatct agatgagcaa ttcgctaaga ccaaagagca cttgactgag
     3181 actcttaaaa aattactttc agctgatgca gctgtttctg gaaataatga tataattgac
     3241 aattacattg ttgataaaat caagcgaaac aaagaagcct tgatgctagg attaacctat
     3301 ctagaacgct ggtatgattt caaatttgat aaggctagcg caaaagattt gcttatgttc
     3361 catatggatt tctttggtaa gggaaatacc tctcctcttg atacgattat tgaactaggt
     3421 aaatctggct acaacaatct cttggcaaaa aataatgtgg ttacttacaa tgccttgctg
     3481 acgaataact acggtacaaa agatctgttt agtgctttag aaggctatcg taaagcattt
     3541 gcacctactc aaactaacaa tgactggttt aaatcacaga ccaaggctta tatcgttgaa
     3601 gaaaaatcta atattctaga agtaaaagct aatcaggaaa aagctggtag caagtactct
     3661 atcggtgtct atgatcgaat cactagcgac agctggaaat accgtaacat ggtactacct
     3721 ctacttacct tgccagaaaa atcagtattt gtcatttcaa ccatatctag tctaggtttt
     3781 ggtgcttatg atcgatatcg taataaagaa catcaagcaa gtggtgatct aaatagcttt
     3841 gttgaagaaa acgcgcgtga gacagccaaa cgtcagcgag atcactatga ttattggtat
     3901 cgtattctag atgaaaaaga gcgtgaaaag ctttatcgaa atatcttgct ttatgatgct
     3961 tataaatttg gtgatgatca taccgaagga aaggctaaaa aagtagcaac ttttgatgat
     4021 ccaaatcctg caatgcaaca tttctttgga cctgttggaa ataaggttgg tcataatgaa
     4081 cacggtgctt acgctacagg ggatgctgtt tattatatgg gttatcgtat gttggataag
     4141 gatggtgcca ttacctatac tcatgaaatg acgcacgatt cagaccagga catttatcta
     4201 ggcggatatg gcagaagaag tggactggga ccagagttct ttgctaaagg attactacaa
     4261 gcgccagacc atccaaatga cgcaaccatc accatcaact cgatcttaaa acattcaaaa
     4321 tcggatagta cagaaggaca acgattacaa gtacttgatc caaccacgag atttaataat
     4381 gcagatgatt tgaagcaata tgtccacaat atgtttgatg ttatttatat gttagaatat
     4441 cttgaaggaa aatcaataat cagtcaacta tctgcaacag aaaaaatgac ggccctgaga
     4501 aaaatcgaga ataaatacgt gaaagatcgt gaagatggaa atgaggttta cgctactaac
     4561 gtagtacaaa atctgacaga agaagatgcc aaaaaactga caagctttga gaatttgatt
     4621 gataataaca tcctttcagc tcgtgaatac aagtctaaag aatacgaaag aaatggctac
     4681 ttcactatta agttatttgc accaatttat gcagcattaa gtagtgatat aggaacacca
     4741 ggtgacctga tgggacgtcg tatagcctat gaactactag ctgctaaagg ctttaaagat
     4801 ggtatggtac catatatttc caaccaatac gaagaagttg ccaaacaaaa tggtaaaaag
     4861 ataactattt atggtaaaga acgcggattg gtgacagatg aacttgttct tcaaaaagta
     4921 tttaacggtc aatatgaaac ttggacagaa ttcaagaaag ctatgtataa tgaacgtgta
     4981 gctcaatttg atagactgaa taaagttact tttaatgata caacacagcc ttggcaaaca
     5041 tttgccaaga aaactacaag cagtgtagat gaattacaga aattaatgga cgttgctgtt
     5101 cgtaaggatg cagaacacaa ttactaccat tggaataact acaatccaga catagatagt
     5161 gaagtccaca agctcaagaa agcaatcttt aaagcttatc ttgaccaaac cgatgatttt
     5221 agaagttcaa tttttgagaa taaaaaatag tgtttactat taggaaataa aaagtgaatg
     5281 tagagaatta gaattgtcat tattaaaaaa gataaatttt ccattcggaa aataaagggt
     5341 attgttggtt cggtattctt ggggagtctt ttattcgcac cgtcagttgt tggtgcatcg
     5401 ccttatcatt acttagatta tagtaattta acacaaaatg agcgtgctca actgaaatca
     5461 ggtagacctg ataaatcaaa agagtcatat gctttggttt atgaaaaaga cgcattacca
     5521 aacacaggaa gttctcaatc tattatgact gcattgggtt tgttagctat cgggagtctt
     5581 attgtgatta tcacaaaaga taaaaagagg aaaaaaatag ctacattttt gattgtaggg
     5641 gcgacaggtt tagttacact atcaactgct tcagcactta atttgaatgc taacatccat
     5701 gagtctggac gtgatggtgt gttgcaaatt tccagttaca gatatgttgg ttacttggag
     5761 cttgatgata gaactgtttc gtcagtttct ccagcatcaa ctgtttcgcc agttgagcaa
     5821 ccaaaagttg tgactgaaaa aggggaacca gaaattcatg agaagcctga ctatacgcaa
     5881 ccaataggtg caaaccttgt tgagcctgaa gttcatgaga aactagcata cactgagcca
     5941 gtaggtacaa cagaagtgga tgaaaatgga aacttgattg aaccacctgt tagcgaaaaa
     6001 ccagagtaca ctcaacctgt aggtatggtt cctgacgaag cacctaaaac tgagaaacca
     6061 gaatatactg agccagtagg tacagcagga gtagatgaaa atggaaactt gattgagcca
     6121 cccgttagca acattccaga gtatactgag ccgatatcta cagtttcaga agttgcatca
     6181 gaaagagaag agtcaccttc tttacatacc gatattcgga cagagactat tcctaaaacc
     6241 actatagaag aatctgatcc taccaaattt ataggcgata attctgttaa acaaattggt
     6301 gaggatggcg aacgtcaaat tgttactagc tatgaagagt tacatggcaa aaaaattagt
     6361 gaaccagttg aaacagtaac aattttgaaa gaaatgaagc ctgaaattct tgtaaaaggt
     6421 acaaaagaaa aaccaacggt tgatttaaaa accgataact tatctcaagt cttagagaac
     6481 ttggattatt acaaagatta tacgatttca actactatga cttatgatgt aggtaagggt
     6541 gcagaagtat cgactttgga agatagacct ttacgtttag atttaaagaa agttgagttg
     6601 aaagatattg caaacacaag tctcgtacaa gtgaacgaaa gcggtgtcga aagtgatagt
     6661 aatcacttaa cctctcttcc aagtgatgtt accaactatt acttaaaagt aacctccaga
     6721 gaaaacaaag tgacccgttt agcaattgat aagattgaag aggtcattga ggaaggtaag
     6781 caactttaca aggtaacagc aaaagcacct gacttagttc aacgtgacaa agacggtaag
     6841 ttaagagaca cttacactta ttattttgaa aaaccgaggg ctaccgagga taaggtttat
     6901 tacaacttcc atgatttagc aaaagatatg caagcaaatc ctaccggtga gtttaaactt
     6961 ggtgcagatt tgaatgcagt taacgttaag ccagcaggta aagcttatgt tatggttaag
     7021 tttagaggta ctttatcaag tgtagagaat catcaataca cgattcataa cttagaaaga
     7081 cctttgttta atgaagctga aggtgctaca ctcaaaaact ttaacttggg taatgtaaat
     7141 atcaacatgc cttgggctga taaagttgcc cctatcggta atatgtttaa gaactctaca
     7201 cttgaaaata tcaaagtagt aggttcagta acaggaaata acgatgtaac cggtgctgtt
     7261 aataagttag atgaagctaa tatgcgcaat gtagcattta ttggtaagat taacagtctt
     7321 ggagataaag gctggtggtc tggtggactt gttagtgaaa gttggagaag taatgtcgat
     7381 agctcatata ttgatgctga tatcaaagcc aacaactcta aggtcggtgg gctaattgct
     7441 aaaattgacc atggtgtaaa ccctatggat gtaaaacaac gaggtcgctt aacgaaatct
     7501 gttgttaaag gtactatgac acttaaaaat catggtcaaa gtggtggtgt tattcacgat
     7561 aactacaatt ggggatgggt tgaaaacaac gtcagcatga tgaaagtaaa caatggtgaa
     7621 atcatgtacg gttctggttc agtggattcg ggcgacccag actttggatt ccattacttc
     7681 aagaacaatg tatatgtgag agatgttgcg tcaggcaatg tttcatataa acgttcaaaa
     7741 caaattcagg gtgtagacca agctgaagct gataaacgta ttgccacctt caatatcact
     7801 gctgataagt atgaaatcac ggatccactt gtaaacacac tcaacaacct gacaactcgt
     7861 gataatgagt ataagacaac gcaagattac aaagctgaac gtgaacaagc ctatcgcaat
     7921 attgaaaaac ttcaaccgtt ctataataaa gagtggatcg taaaccaagg gaataagtta
     7981 acagatgagt ccaatcttgt taagaaaacc gttctgtctg taacaggtat gaaagcaggg
     8041 cagtttgtaa ctgatttgtc agatattgat aagattatgg ttcactatgc tgatggtaca
     8101 aaggaagagt taactgtaac tgctaaaact gactctaaag tagctcaagt aaaagagtat
     8161 gatgtagcag gtcaaaacat tgtctatact ccaaatatgg ttatgaagaa tcgtaaccag
     8221 ttagcaagcg gtattaaaga gaagttagct agtgttacct tactttcaga tgaagttcgt
     8281 gctttgatgg ataaacgtga gaagccttta atatactcct gagaagaaga cggagtatat
     8341 taaaggcttg tacttagaag aaagtttcgc agaactaaaa ggaaacttag aaaaacttgt
     8401 tagtcagatt ttagaaaatg aagatcatca gttaaatggg gggaagcagt cgagcgtgct
     8461 ttactgaaga aagttgaaga caacaaagct aaaatcatga tgggacttgc ctacttaaat
     8521 caatattatg gttttaaata cgataagtta tcaattaaag atattatgat gtttaaacct
     8581 gatttttatg gtaagaatgt taatatctta gatttcttaa tcaaaattgg ttcaagtgaa
     8641 agaaatgtta aaggtgatag aactttagaa gcttatcgtg aaactatagg tggaacaatc
     8701 ggaataaatg aattaaatgg attcttacat tacaatatga agctattgac taaccataca
     8761 gatataaatg actggtttaa gaaagctatt gaaaagaatg cgtatgtagt tgaacaacca
     8821 tcaacgaacc ctgcatttgc taacaaaaaa tatcgtctat acgaaggaat taataatgga
     8881 cagcatgggc gtatgatttt acctcttctt aacttgaaaa atgcacattt atttatgatt
     8941 tcaacgtata atactatctc atttagttcg tttgaaaaat acaacaaaaa tactgaagaa
     9001 gaacgtgaag cctttaaaaa agagattaat ctacgtgcca aagagcaagt taattactta
     9061 gatttttggt cacgtcttgc tactgataat gtccgcgata aacttcttaa gagtcaaaat
     9121 gtagttccaa ctcctgtatg ggataatcat aacgctccag gtggctggcc tgatagattc
     9181 ggacatagaa atggaaaacc agactataat ccagtcaggg aattctttgg tcgtattggg
     9241 aagtatcatc cttaccaata tggatacgga gcatatgcat atatttttgc agcaccgcaa
     9301 ccgatggatt cagtttattt cgttatgaca gatttaatta gtgattttgg aacatccgca
     9361 tttacacatg aaactactca cgttaatgat cgtatggttt actatggtgg tcattggcat
     9421 cgccaaggta cggatttaga agcctttgct caaggtatgt tgcaaacgcc atcggtatca
     9481 aatccaaacg gagaatatgg tgctttaggc cttaacatgg cttatcaccg tgaaaatgat
     9541 ggtaaccaat ggtacaatta taatcctgat aagttacaaa ctcgtgaaga catcgatcgc
     9601 tacatgaaga actacaatga agcgttaatg atgttggatt atgttgaagc agatgctgta
     9661 attccaaaat taaatggtga caatagcaaa tggttcaaga aaattgaccg tgaaatacgt
     9721 cgaccaatgg atagaaataa actaagtgct cctcaccaat gggacaaagt gcgtgacttg
     9781 acagacgctg agagaacaac accactgaat tccattgatg acttagtaaa taataatttc
     9841 atgaccattc atggtaatcc aggtaacgga cgttatagac cagaggattt tacaccaaaa
     9901 tcagcttacg taaatgtcaa tatgatggca ggaatttacg gtggaaatac gagtgatggt
     9961 gcacctggtt cattatcgtt taagcacaat gctttccgta tgtggggtta ctatggttat
    10021 gaaaatggat ttatcagtta tgtgtcaaat aaatacaaag cagaagcgga taaaaacaac
    10081 catggtttac taagcgataa gctaataatt actaaagtat caaaaggaaa ctttagtacc
    10141 cttgaagagt ggaagagaca ttggtacgaa gaagttcttg ctaaagctaa gaaaggtttt
    10201 gaggctatcg atattgatgg agtccatatt agcaactatg atgagttgcg cactttattt
    10261 gctgaagcag ttcaaaaaga tcttgatggt atgtcagatc caaaaattaa aaatcatttc
    10321 aaaaatacag tggacttgaa atccaagatc tttaaagccc ttcttaaaaa cacagacggt
    10381 ttctttaatc cattgttcaa aaaagacatt taaatcttta aaatataaag ggaacagttt
    10441 tttgctccct ctgaaaagtc atcatttgat ggcttttttc tatatagggt aaaagatagg
    10501 gtaaaaggct atcatcggac aaaataaaga aggcatgata taatataaag tagatttcta
    10561 tgtcataaaa caagaactgt ttggacatca ttcatttgaa aactctctat gttcaaacaa
    10621 tagtaaaata aaatagggga tctaaatcct tgctacgaaa ggaaaaaact caatggctac
    10681 tattcaatgg tttcctggtc acatgtctaa agcgcgtcga caggtgcagg aaaatttaaa
    10741 atttgttgat tttgtgacga ttttagtaga tgcacgcttg cctctatcta gtcaaaatcc
    10801 tatgttgacc aagattgttg gtgataaacc aaaactcttg attttaaaca aggccgactt
    10861 ggctgatcca gcaatgacca aggaatggcg tcagtatttt gaatcacaag gaatccagac
    10921 gctagctatc aactccaaag agcaagtgac tgtaaaagtt gtaacagatg cggccaagaa
    10981 gctcatggct gataagattg ctcgccagaa agaacgtggg attcagattg aaaccttgcg
    11041 taccatgatt attgggattc caaacgctgg taaatcaact ctgatgaacc gtttggctgg
    11101 taaaaagatt gctgttgttg gaaacaagcc aggggtcaca aaaggtcaac aatggcttaa
    11161 aaccaataaa gatctggaaa tcttggatac accggggatt ctctggccta agtttgagga
    11221 tgaaactgtt gcacttaagt tggcattgac tggagctatc aaggatcagt tgcttcctat
    11281 ggatgaggtt accatttttg gtatcaatta tttcaaagaa cattatccag aaaagctggc
    11341 tgaacgcttc aaacaaatga aaattgaaga agaagcgcct gtgattatta tggatatgac
    11401 ctgcgccctc ggtttccgtg atgactatga ccgtttttac agtctcttcg tgaaggaagt
    11461 ccgtgatggc aaactcggta actatacctt agatacattg gaagacctcg atggcaacga
    11521 ttaaagaaat caaagaactc cttgtgacag tcaaggagtt agaaagccct atttttttag
    11581 agcttgaaaa ggataatcgc tcaggagttc aaaaggaaat cagcaagcgt aaaagagcca
    11641 ttcaagctga attagatgaa aatttgcgct tggaatccat gctttcttat gaaaaagaac
    11701 tttataagca aggattgacc ttaattgcag gtattgatga ggttggtcgt ggtcctcttg
    11761 ctggtcctgt agtcgctgcg gccgttattt tacctaaaaa ttgtaagatt aaaggtctca
    11821 acgacagcaa gaaaattcct aaaaagaaac atctggagat tttccaagcc gttcaagacc
    11881 aagccttgtc gattggaatt ggtatcatag ataatcaggt catcgaccaa gtcaacatct
    11941 atgaagcaac caaactagcc atgcaagaag caatctccca gctcagccct caaccagagc
    12001 accttttgat tgatgccatg aaactggact tgcccatttc acaaacctcc attatcaaag
    12061 gagatgccaa ctccctctct atcgcagcag catctatagt agccaaggta acacgtgatg
    12121 aattgatgaa ggaatacgat cagcagttcc ctggctatga tttcgctact aatgcaggat
    12181 atggcacagc taaacatctg gaaggcctca caaaactagg agttacccca attcaccgaa
    12241 ccagctttga acccgttaaa tcactggttt taggtaaaaa agaaagttaa ttgaaaggaa
    12301 ataccatgga ggaacagtcg gaaatagtcc gttctaagaa agaattcgcc tttgcatcca
    12361 gcactatact atcccaagtt ggtcgaggaa tcattgtcgg cctcatcgtt ggaattatcg
    12421 tcggatcctt tcgtttctta attgaaaagg gcttccacct gatacaagga gtttatcaag
    12481 atcaagggta cttagtgcgc aatctttttg tactggtttt gttttatata ctcatctgtt
    12541 ggctcagtgc caaactaaca cggtcagaaa aagatattaa aggctcagga attcctcaag
    12601 tcgaagccga actgaaaggc ctcatgtccc tcaactggtg gggcattctt tggaaaaaat
    12661 atgtgctagg tattcttgct attgccagtg gactcatgct gggtcgagag ggacccagta
    12721 ttcaacttgg agcagttggt ggtaaaggaa ttgccaagtg gctcaaatcc agtccagtag
    12781 aggaacgctc cttgattgcc agtggagctg cagcaggttt agccgcagcc tttaatgctc
    12841 ctattgcagc acttctcttt gttgtagaag aagtctatca ccatttttcg cgctttttct
    12901 gggtctcaac tctagcagcc agcatcgtag caaactttgt gtctctactc atgttcggtt
    12961 tgacaccagt attggatatg ccagataaca ttcctcccat gaccctagat cagtattgga
    13021 tatatctcgt catgggaatt ttccttggat tttcaggctt tctctatgag aaagctgtat
    13081 taaacgttgg aagagtttat gacttgattg gtcaaaaaat ccatttggat agagcttatt
    13141 atcccatctt ggcttttatc cttatcatac cagtcggaat cttcttacct caaatccttg
    13201 gtggcggaaa tcagcttgtc ctttctttaa ctgaacaaaa ttttagtttc caagttttat
    13261 tagcttactt tttgatccgc tttatttgga gtatgattag ctatggaagt ggactgccag
    13321 gaggaatttt cctccccatt ttagctcttg gttctttgct tggtgcctta gttggtgtta
    13381 tctgtgtcaa tcttggactt gtcagtcaag agcaattccc tatatttgtc attctaggaa
    13441 tgagtggcta ttttggagcc atatcaaaag ctcccttaac cgctatgatc ctcgtaactg
    13501 agatggtagg agatattcgc aacctcatgc cacttggtct tgtcactctt gtttcttata
    13561 ttatcatgga tttgctcaaa ggtacgccag tctatgaagc catgctggaa aaaatgcttc
    13621 cagaagaagt atctagcgaa ggagaagtta cacttatcga aataccagtt tctgataaaa
    13681 ttgctgggaa acaagttcat gaactcaact taccacacaa cgtcctcatc acaactcaag
    13741 tccataatgg caagagccaa acagttaacg gctcaaccag aatgtatctg ggtgatatga
    13801 ttcacctggt tattccaaaa agtgaaattg gaaaagtcaa agatttgttg ttgtagtatg
    13861 attatttaca taatttatgt tatgtaaata atcagcttga tttatttaga aaaccgattc
    13921 tcagaaatga gatcggttat tttttactga tgaggaattt tacatataaa taattgaact
    13981 ttattaaaaa taagactata attaagttag aaatgataaa gtataaagct agaaaggagt
    14041 ttactgtatc aaatctgtac agtaagatta aaatcatgaa aaagaaaaca atagcaatta
    14101 tatagtgaaa tgaaatagaa atatgataaa acaatcagga caatcaaatc aatttctagc
    14161 aatgttttag aagtccagat gtactattct agtttcaatc tattatacaa tgtgttttgt
    14221 atctcatagc tccttatata gctcttcagt tatgtagtat taacagaagt ttagtgggtg
    14281 agatttttat tattttcctt attctgtttt gtttgtaggt ctaagtcttt ttatcacttt
    14341 gaaaaactcc tataacatct ttctgaaaaa ctataatttt cttgaaaaat atacaagtct
    14401 atgctatact actagtatac ttacttatgg agaaaataca tgaaacgtga gattttactg
    14461 gaacgaatcg acaaactaaa acaactcatg ccctggtatg ttctggaata ctaccaatct
    14521 aagctggctg tgccctacag ttttacaacc ctgtacgaat accttaagga atatgaccga
    14581 tttttcagct gggttttgga gtctggtatt tcaaacgctg ataaaatatc cgatattcct
    14641 ttatcagttt tggaaaatat gtctaagaaa gacatggaat cctttatcct ttatctacgt
    14701 gaacgtccct tgctgaatgc taatacaaca aaacaaggtg tttcacagac aactatcaat
    14761 cgaaccttat cagcactttc tagtctttac aagtatctaa ccgaggaggt tgaaaacgat
    14821 cagggggaac cttatttcta tcgtaatgta atgaaaaaag tttcaaccaa gaaaaagaaa
    14881 gaaacccttg ctgccagagc tgaaaatatc aagcaaaaac tctttctagg tgatgaaaca
    14941 gaaggttttc taacttatat cgatcaagag cacccacaac agctttcaaa tcgagctctc
    15001 tcatcattca acaaaaataa agaacgagat ttagccatta ttgcccttct cttggcatct
    15061 ggtgttcgct tatctgaagc tgttaatcta gatctaagag atctcaatct aaaaatgatg
    15121 gttattgatg ttactcgaaa aggtggcaaa cgtgacttag tcaatgtcgc tgcttttgct
    15181 aaaccttatt tagagaatta tctggccatt cggaatcaac gctataaaac ggaaaaaaca
    15241 gatacagccc tttttttaac tctctacaga ggtgttccta atcgtatcga tgcttctagc
    15301 gttgagaaaa tggttgctaa atactcagag gattttaaag tacgtgtaac cccccataaa
    15361 ctgcgccata cgctagcaac taggctctat gatgcgacta aatcacaagt tttagtcagt
    15421 caccaactag ggcatgctag cacacaagtc actgacctct atacccatat tgttagtgat
    15481 gaacaaaaga atgctctgga tagtttataa ttttacgtat tttaaattat gtaaataaat
    15541 atcaaaaaaa gaagttggtt agttggccaa cttctttttg aagtacgacg ggcatgtcgt
    15601 gcatctgagg tgtaagtcct ccgtgggcac ccgctaccgg tgaacccaat agcgattccc
    15661 aagcctgact atcgtgaggt agcagggaga ggaagggata gcgaaatcgt ggctctacga
    15721 acaggaacgt gatagtaagg cgtatatagc ggataagggg cctcaaactc taaagtccaa
    15781 aaaggtagtc gtaacctata tgcgtaaatc acgagagtaa ttgaattcgg actaaggttt
    15841 gtgtgaaaaa gataaatctt tctagagtct aaagactctg cgtcagattt cctattttca
    15901 ctgtaacctt ttaacgtcct catatcttgt ataaacgagg aaagatgtac gacttatccc
    15961 gtgaggtctc atgagcgatg aaagcgtagt aacaacgaat catgagaagt cagccgaacc
    16021 catagtagtg aggaaacttc tgtaatggaa gtggagcgaa ggggtgaaca actaattgaa
    16081 gtaatcctac ttcaattgtg tctgtaaaac aagcggtctt atagaactgg cctctgtcac
    16141 gtattgacta gatgaaggtt caccaatata agatatccct caggcaccaa aacaagaaag
    16201 gaatacgcac atgtcaaaat tgctagataa gatattatca cgcgaaaata tgcttgaagc
    16261 ctacaatcaa gtaaaatcca ataaaggctc agctgggatt gatggaatga ctatcgaaga
    16321 gatggataat tatctcagac aaaactggcg cttgactaag gaactgataa aacagagaaa
    16381 atataagcct caaccagttc ttagagttga gattcctaaa ccaaacggag gcatccgtca
    16441 actaggaatt ccaacagtta tggatagaat gattcaacag gccattgtcc aagtcatgag
    16501 ccccatttgt gaaccccatt tctcagatac gagttatggt ttcagaccaa ataggtcatg
    16561 tgaaaaagcc atcatgaagt tcttagagta cttaaatgac ggctatgagt ggatagtgga
    16621 tatagaccta gagaaatttt tcgatacggt tcctcaagat agattgatgt ccttagtaca
    16681 taacattatc gaagacggag atacggaatc cttgattcgt aagtatcttc attcaggtgt
    16741 tatcattaat ggtcagcgtc ataaaacgct agttggcacg ccacagggag gaaatttatc
    16801 tcctctctta tccaatatca tgcttaatga attggacaag gaattagaaa agaggggact
    16861 tcgatttgtg cgctacgcag atgattgtgt gattacagtc ggaagcgagg ctgccgctaa
    16921 gcgtgtgatg tattcagtca gttattttat tgagaaacgg ctaggtttga aagtaaacat
    16981 aaccaagact aagattacca gaccaagaga gttgaaatat ctaggtttcg ggttctggaa
    17041 atcatcagat ggttggaaaa gtcgtccaca tcaagatagt gttcggagat ttaagcttaa
    17101 attgaagaaa ctaacacaga tgaaatggag tatagactta acaagacgta ttgagcaact
    17161 gaatttgtcc attcgaggct ggataaacta tttctcattg gggaatatga aaagtatagt
    17221 cactagcata gatgagcgct tgcgtactcg cctacgagtg attatctgga agcaatggaa
    17281 gaagaaatcg agacgattat ggggattgct taagttagga gttcctaaat ggatagcaga
    17341 taaggtatct ggctgggacg accattatca attagtagct cagaagtagg tacttaaacg
    17401 tgctatatca aaaccagtcc tggaaaaacg tggactggtt tcgtgtttgg attattacct
    17461 tgaacgacat gcgttaaaag ttagttgaac cgccgtatgc cgaacggcac gtacggtggt
    17521 gtgagagggg ctagagatta tcccctactc gatttatcca actaccgctt cagcgatttc
    17581 ttcacggcta ataccagcga agtagcgtgt gatatcaatg gtttttagcg ccttaagaac
    17641 atcttcgcgt tcgtatttca ccccacgaag gacatcttct actgcagcaa cgtcttcaat
    17701 accaaagaag tcaccataaa tcttgatgtc ttggattttt gattcagtaa cgttagcaaa
    17761 gacttcaacc ttaccactag tgaatttggt tccacgacgg acgttaaatt caggtgattt
    17821 accatagttc cagtcccaag ttccaaactt agtatccttg atgcgattga tttcggccaa
    17881 ttcttcttct gaaaaaatgt atttcatgat atccctttac tttatatgat atatgataga
    17941 aactggaaat aaccttccag tctaatctat tttcgtttta ttttttctta ggtgaatgga
    18001 tggccattcc tagaacatct gcaaacgctt cgtacatcac ttcagagtaa gttgggtgcc
    18061 cgtggatggt cttcagcatt tcctcaacag tgatttccat ttcgatgatg cttgatgctt
    18121 cgtttattaa ttctgcggct gcaggaccaa taatgtgtac accaaggatt tctccgtatt
    18181 tcttatcagc gataactttt acgaaacctt gagcagcgtc agatgcaata gcacgaccgt
    18241 tagcagcaaa gttaaactta ccgatggcaa catcgtattt ctcacgggct tgttcttctg
    18301 tcaaacctac tgctgctact tcagggagag tgtagatggc tgcaggagtc aaattcaatt
    18361 tggcaactgc atgatttcct ttaagggcat tttcagcgga aacttcaccc atgcggaaag
    18421 ctgcgtgagc caacatctta gtaccgttga tgtcacctgg tgcataaatg cctggaactg
    18481 aagtttccat gtattcgttg accttgatac aaccacgatc caattcaaac tcaacctctc
    18541 caataccttc aaggtctggc atacgaccaa ttgaaagaag agctttggtt gcgatgatat
    18601 cgtcttttcc ttcaaccttg atacgaagtt gaccattttc ctcaatgatt tcttgcagtt
    18661 tagtaccagt caagatggtc attcctttac gctcaaggat caagcgaagg ttcttagaaa
    18721 cttccgcatc cataactgga actatacggt ccatcatttc gataacagtc acttttgaac
    18781 caaatgtcat gaaggcctga ccgagttcga taccgacaac tccaccaccg atgataacaa
    18841 ggctttctgg cacttcgttc atttcaagaa tgtcatcact agtcatgaca agtggagatt
    18901 ccataccagg gacgttgatc ttgctgactt ttgaaccacc agcaagaatg attttcttgg
    18961 tttcaagcaa ttcagaacca tttaccaaga cgttcttgtc tttagtgatt gtaccaattc
    19021 ctttatgaac agtaactccg tagctacgaa gaagtcctgc aacaccacca acaagagtat
    19081 taacaacttt agatttagtt tctaaaagtt tttccatatc aacagtgaag ttaggatttt
    19141 caatcacgat accacgattt gcagcatgac cgatattttc aataatttca gcgttatgaa
    19201 ggtaggtctt ggttggaata catccacggt ttaagcaggt tccaccaagt tcagatttct
    19261 caacaagggc aaccttaccg ccgaattggg cagctttaat ggctgcaaca taaccagcag
    19321 gacctccacc aatcacaacg atatcaaaag catcatcgct cttaccatca tcgtttgagg
    19381 tacttgctac aggtacaggg ctagcttctg gcgatgctgc tccagctgtt gggatgtttt
    19441 ccctttcttc accaaggtaa ccgataactt ccgttacagg gacagtttca ccatctcctt
    19501 tgagaatggc aatcaagtac ccatcttctt cggcttccaa ttccatgctg actttatcag
    19561 tcatgatttc caaaaggatt tctccttctt ttacaaattc tccgactttt ttattccatt
    19621 ggacgatttg tccttctgtc atatccacgc cgacttttgg cataattact tctaaggcca
    19681 tgtcttgctt cctttatcta tatcttaaaa atgaatactc tttctcttaa attaacattg
    19741 agattggcgt ttcaatcaac tctttcaagt ccttcataaa cttagcacca gccataccat
    19801 ctacgacacg gtggtcaatg gttaatccta aactcatgat tgggcgaatc acaatttcac
    19861 cattgacgac aactggcttc tcgattgtcg aactgacacc aaggatagct gagttgggtt
    19921 ggttaataat cggaccaaag gactgaacac caaacattcc caaattactg attgtgaatg
    19981 ttgaattttg taactcactt ggagccaatt taccatccaa ggtacggcca ataacatcct
    20041 taaaggctac aaccagttct gaaagactca tcttctcagc attgtaaaca acaggtgtca
    20101 tcaatccatt atccatccca actgccatgg caagattgac atagttgtga gtgataatag
    20161 tcttgccatc ttctgtcaat gaagcgttga tgtatgggtg tttcataaga gtcttaacaa
    20221 ctgcaagcga aagaaggtct gttacagtag tcttcttccc agttgcttcc atgattggct
    20281 caagaacctt cttacgaaga gccaacattt cagtcatatc aacttcatag ttgagggtga
    20341 aggttggcgc agtcaagtaa gattcaacca tgcgttgggc aataacctta cgcattggtg
    20401 tcattggaat acgctcaatt tcactatatg gtgttacgtt atcagggact tcttccactt
    20461 tttcaatctg agcaggagat ttgatgctat cgttttcgat attttcagga agcagggcca
    20521 aaacatcctt cttcatgatt ttaccacgat gaccggttcc ttggatttcc tgccaagcaa
    20581 tgttatgttc gagggcaatt cgttttgcaa gtggcgaaat gcgaaccacg tttgtgtctt
    20641 tataagtttc cacgtcttct ttgtggacac gaccgtttgc acctgagcca gaaacgtcgt
    20701 agaggtttat ccctaaatca tccgctaact ttctagctgc aggagtcgct cttagcttgt
    20761 catcagccat gatctctcca attctattta tgatacaaag ggcgtcaaaa gcgactgaaa
    20821 aataggaaat ctccttggct tcgatgaagc caaggagatt tatctttttt ccgagctttt
    20881 agcccgtgct ctaatctaag atattaatga cgaagagctc tgcacctaaa agatataaag
    20941 tttctcgtca gctttgtttt agttacataa cttatattat gtaactctat tctttgttat
    21001 aagtttttcg gattgcatct ttgatacttt caactgttgg aatcattgca ttttctaggt
    21061 tttgtgcata aggcatcggc acatcttctc ctgcacaacg gcggattggt gcatctagat
    21121 agtcaaatgc ttctgattct gaaataatag ctgaaatttc accgatatag ccacttgttt
    21181 tgtgggcatc gttgaccaga acaaccttac cagtcttctt cactgagttt atgatgatat
    21241 ccttatcaag cggaacaagg gtacgtgggt caacaatttc aactgaaatt ccttcttctg
    21301 ctaattcttc agcagcttga accacacggc gaagcatttt tccataagta acaactgtta
    21361 catccgttcc ttggagtttg atttcaccaa ccccaagtgg aattgtgtag tctggatcaa
    21421 ctggcacttc ccctttttgg ttaaattctg acttgtactc aagtataata actgggttgt
    21481 tatcacggat agaagactta agcaggcctt tcatgtccgc aggtgttcca ggtgccacaa
    21541 ccttaagtcc tgagattgtg agcgctgggc ggcagagcca actccgttac cagctgcaca
    21601 acgaacagtc attggaacct gacctttacc accaaacatg taacgtgttt tagcagcttg
    21661 gttgacgata ttgtccatgg caataacaga gaagtccatg aaggtcatat cgacgattgg
    21721 acgaagtcct gtcatggctg ctcctgctgc tgctccagag atggcagctt cagaaatcgg
    21781 acagtcacgg acacgttctg gaccaaattc ttcaagcatt ccaacagaag taccgaagtc
    21841 tcctccaaag acaccgacgt cttctcccat caagaacaca ttttcatcgc gacgcatttc
    21901 ctcagacata gcaaggataa tggtgtcacg gaaggacatt gtttttgttt ccattttatc
    21961 tctttctcct tagtctgcgt aaatatcttc aaagactgat tcaagcggtg ggaatgggct
    22021 ttcctctgca aatttaacag aagcttctac tgcttccttt acttgcgctt ggatttcttc
    22081 caattcttcg gcacttgcaa tgttattttc aataaggtaa ttgcggaggt tttcgattgg
    22141 atctttttgt ttccacaatt ccacttcttc acgcgtacga tatttaccag ggtcagatga
    22201 tgagtgaccg agccagcgat aagttacact ttcaatcaag actggaccat tgccactgcg
    22261 aacatggtct acagctttct gaaatccttc atagacatcg atgacattgt taccgtcttc
    22321 gatgaacatt ccaggaattc cataagcggc gctacgttga tggatatgtt ctatattggt
    22381 cattttcttg atatccgcag agataccgta accgttgtta atgcaataga aaatgactgg
    22441 caggttccag atagaagcca tgttcactgc ttcgtggaaa acaccttcat tggtcgcacc
    22501 atctccaaag aagcaaacaa cgattttacc ggtattttgc atttgctgac tgagggctgc
    22561 accgacagcg atccccatac caccacctac gataccattg gcaccaaggt tcccagcatc
    22621 aaggtcagcg atatgcatag ttccaccttt ccctttacag gttccagtgt atttaccaag
    22681 gatttcagcc atcattccgt tgaggtcaat ccctttagca atagcttgcc cgtgtccacg
    22741 gtggtttgag gtaatcagat catctggatt gagagctaac atagccccca cgttagctgc
    22801 ctcttcacca acagaaaagt gcgtcattcc tggcactttc cctttcttta ctaattgtgc
    22861 aatttttaag tccatgcaac ggatttcttc catcttacgg aacatttcta gcaaaagatt
    22921 tttatctaaa gttgacatct tcttgccttt ctaactttct tcttacctta ctattttacc
    22981 gcttttggca aatactgtca aagtttttct aaaagaaatt tcacaaaata aaaaagaaaa
    23041 ccccgtaaaa acaagggatt ttcttgtcaa gaatattttt tcacaaactt tttagcgttt
    23101 ggattttgct aaagattcaa atctcttcat aatcacagtt aaacgccaac ggtagagcgc
    23161 cccgctcaca atcaaactaa taatcaagcc gatccagtaa gaataagctc caaaatctgt
    23221 tagggaatca aatagcgtag ccacagggat tgctacgccc caataaccaa gcaaaccaag
    23281 gtaaaaagga ataactgtat ccttataccc ccgcaaaatt ccctgaagcg gcgccgcaaa
    23341 ggtatctgct aactggaaga aaagactata agttaaaaaa cgcgctgtca aatcgataaa
    23401 ttttgggtcg ttaccataaa gactggccac atttccccta aaaatgtaaa ggaaggttaa
    23461 ggtgaaggcc gcaaaaatga gggcagtcca tcttcctaga ccaatatagg ttttcgcatc
    23521 atcaaatcgc ttggctccca cttcatagga aacgacaata gccatagccg atgagatact
    23581 cataggaaag gcgtacataa gacttgaaaa gttcatagct gactggtgac tagctataat
    23641 caaggacgaa aacttagcca taatcaagcc aactactgaa aagatagcca cttccgcgaa
    23701 gacagttccc ccaataggca gacctaaacg aacgccttcc ttaattttat ccatattaag
    23761 tggaattcgt ttctcaaggt gtaaggcttt gagcttctcc tgtttaaata aaaccagaac
    23821 agaaatccca agcaagaccc agtaggccaa ggatgttcct aaaccagcac cagcccctcc
    23881 cagttctgga acaccaaagg caccgtaaat caagagatag ttaaatccgc tattgagagg
    23941 gagtaacaaa agcatgaggt acatggacag tttggtcaag cctagcgaat ccagcaagga
    24001 acgaatgacg ctaaagagca acaaggggat aatcccgata gataaaaacc aaagatagcg
    24061 aaccgctact gccgctactg ctgcttctaa cccaatatga ttcaagattg ttggtgccaa
    24121 gaaaagtacc atccccagca agaccacaga taggcccaag gccaaataaa taaattggta
    24181 aaaatcagac gcaacttctt cctttttgcc tcgaccaaga tggtgaccaa tgataggcac
    24241 caaggctgac acaatccctg ttagaaatgt aaagaaagga ttccagatac tggttgccat
    24301 agatacacca gccaagtcca tagtgttgta ttgacctgtc attgcagtat caacaaaaga
    24361 ggcagaataa ttggcaaatt ggtagataag gattgggaag aaaattttta aaaataatac
    24421 taacttctct cgtaaacact ttgtcttata catacttctc tttctattct gatttatcta
    24481 aaccaaagag tttcagacca tagtttttca aacttagcgg aggtttatta gattttgaag
    24541 tagtatgcca acacgcacat gtacgacaat aatagcttct aactaaacct ccgttatcat
    24601 attgaaccgc atggtcagct ttttctttag tttcatattg aattttagaa cgattagatg
    24661 cgggacagta aattccgcta ttagattttg cttgtctctc cctacgtttt cgaaaataat
    24721 tcatattcca actcctatca agcttgatag acgatttgtc ccttacagat ggtgtattta
    24781 acctgccctt ttaaggtttc accgatgaat ggtgaattag ctgctttcga agcaaaatgg
    24841 gagtccacaa ggcggtcagc cttagcatca aaaatagtga tgtccgctgg accattctca
    24901 gccaagtaac ctgcttcaaa attgtaaagc ttggatgggt tgtatgtcat tttttcaagt
    24961 aattccatca agcttaactc accagcttcc accaaataag ttaaaccgag agacagagag
    25021 gtttctaagc cagtcatacc agatggcgct ttggtaatat cctcaacatt tttttcatct
    25081 acatgatgag gcgcatggtc agtcgcaata actgtgatga cacctgactt gagaccttca
    25141 atcacggcac gacggtctga ttccaaacga agcggcggat tcatcttggc attacttcct
    25201 tgtgtcaaaa ggagcgcttc ggtcttagaa aaatgctgtg gcgctacttc tgctgtgact
    25261 tgcgcaccca aaccttgagc aaactccact actttaacac tttcttcctt agacaaatgc
    25321 tgaatatgaa catgggcctt ggttgcatag gcaatcatga catcacgcgc catcatagca
    25381 tactcagcca ccccagtagc accgcagata tgaaaatgtt ctttagcaat attttcgtta
    25441 aagccaagaa caccgttcaa tcctggatct tcctcatgaa ggctaataaa ggtattgagt
    25501 tttttggctt cctccatggc ttccttgaca actttactgc tttcaagtgg aatgccatca
    25561 tctgaaaatc caactgctcc aacttctaaa agtgccttaa agtcagtcaa atcttgccca
    25621 ttaaaatttt tagtaatggt cgcaaccgtc ttaacattaa tcttatcttt ggcagctgac
    25681 tggagaactt cttgcaaagt ctctacatct gaaatggttg ggctagtatt agccatcatg
    25741 acaacagttg taaaaccacc tgcagcggct gctagggcac cagtatgaat gtcttcttta
    25801 tgtgtttgac caggttcacg gaaatgcaca tgaatatcga ccaaaccagg agcaaccaca
    25861 agaccactag catcaatcgt ttctgctcct tcttcagtga tttcaggcgc aattttgata
    25921 attttcccat cttgaactaa gacatcacac acttgatcca aaccagattt gggatccatt
    25981 acacgaccat ttttaattag tagcatctgc tttctccttt attcatagaa atcaacttgg
    26041 gtatccaaca atttatcccc atcataaaca aacttggctg aaaagaaggg tttgtcctct
    26101 aaaagccact caacaaaggt gtggtcacct tcccaagttg gcttgctcaa aacttcatca
    26161 tagggaaccc attctagcgt tccctcgtta cagtcaatta agtcaccctc gaactccgtc
    26221 actttaaaaa cataggtgta ccagtctaaa tctggtgtaa attcaggaaa agtgatgaca
    26281 ccttttagaa ctggcttggc tttgagccct gtttcctcaa ggatttcacg cgctgcacat
    26341 tcctgaggcg tctcaccacg ctctagctta ccacccacac caatccattt cccttcatgg
    26401 acatcatttg gcttcttatt acggtggagc atgagcagtt ctttcccatt atcaatgtag
    26461 caaattgtcg ctaactgagg catattttct ccttatctaa gccaatcgat tggctcttgt
    26521 cctgtctctt ttaataatgc attggccttg gaaaaaggtt tggaacccca aaatcctcta
    26581 taaaccgaca aaggacttgg atgggctgat tcgataatca gatggtgagg attggtaact
    26641 aaggccttct tcttgcgtgc ataagctccc cagagtataa agactactgg tttatctaat
    26701 tgattgacca cctgaatcac agcatccgta aaaggctccc atatctgccc agcatgacca
    26761 ttggcctgta cagcaggaac cgtcaaacaa gcgttaagaa gcaagactcc ttgttcagcc
    26821 caagctgtca aatcatgaga tttcttaacc ccgatatcat ctgacaattc tttcaagata
    26881 ttttgcaagg atggcggagc tgggatagag tcaggtacag aaaaactcaa gccctgcgct
    26941 tgacctggcc cgtgataggg gtcttgccct agaatcacta ccttaacttc ttcaagcggt
    27001 gttgtcaaga gagcctgaaa aaccttttcc ttgggtggat aaatagttcc ctgagcatag
    27061 acctgttcca taaactgatt gattttcccg aaataacctt taggcaattg ttcctttatc
    27121 aaagcatgcc aagacgagtg ttccatagcc aactccttcg tttttactgc ctctattata
    27181 gcaaaaagtg gctatctaaa ccacttttta cagtttaaag gataatactc tttaatatta
    27241 ttaacttttt gttcgtaggc atattttgct acatgatcta ggtatatatc aaaaaagtca
    27301 tgaggaaaat ctcccataca gccattacga ttcatattga ggctatattt tcgattagta
    27361 agataattcg gaaatatatt tcctattact ctaagattat ctacttgtga atcccttctc
    27421 ccaatataat ccccttcttt gactaatgat ggagatttcc aagtaaaaat taaatcgcca
    27481 gagaaatttt tcttatcaaa aaacttaaaa agagattgat aaatactatt tgctaattga
    27541 caagtgtcag atgtaaattc ataattaaca gtttcattaa aatcaacttc agacatttca
    27601 atgtactctt caatagtaat attagctttt tttgtagtca ttagctcgta tcctttaaaa
    27661 tgtaatcttt tcaagaatat ttttatccaa acaatccagc aagtcttggt aagaatggac
    27721 ttcgtaagtt ggctgggctt gtgtgtgatt ttcgaggtga tgagggttgt accagatcgt
    27781 gtcaattcca gcattattgc caccttgaat gtcggcggtt agagaatctc caatcatcag
    27841 cgtcttttct ttgctaaagc cagcaatctg ctgaccgatt ttttcataaa atagagcatc
    27901 aggcttttga gtttgcaatt gctcagagat aaagacttga ttgaaataag gttctagacc
    27961 agattgagcc aaacgtcctg tctgaatggc agtaatgcca tttgtcgcag catacaagtc
    28021 ataatcacga tcaatgaggc tgtccaagag atcatgagcg cccgatagtg tttgtccctg
    28081 ctgggcgagg taaaattggt aacgttgggc aagaaaacta ccatcttttc cctgtccaaa
    28141 atgagaaaat aaacgagaaa agcgcgtgtt aaccagctct tgtttactga ttttcttcag
    28201 ctccaagtct ttccagagag ccttgttcat aggaacgtaa taatctttat aagcctgaat
    28261 atccgcaact ccttcttctt ttagaagttg ggtcaaagcc acatcctcag cagcatcaaa
    28321 atcaagaaga gtgtggtcga ggtcgaagag tagaaatttg taggacaatt tgaggttttc
    28381 ctttctgaaa attcattaag aacattatat cataaagcac ctcatacaat taactaattt
    28441 aatcacttga aaaaaattcg aacactatcc tatacaattg acagctcaaa tctttcagaa
    28501 tagaacaatc ctaactatcg aacaccctga cttaataaat atccatgtga ttctaaacct
    28561 agaattccta taaactaaat gttttcgtac tcttctaagt aattgattgc cttaaacttt
    28621 aatttttgaa ggattctaaa gctagaatag tctcatcaca atcagttttg aatgattcac
    28681 aatttagaaa cactataggt ttactcttgt taaaataaaa aggaactgcc caaagcaatc
    28741 cctttttatt tacttaaaat ttgatagttg agataatcaa tagcccacct ataaaaagtg
    28801 ttatagtaaa attccttatt tattgatttc aagctccgac aactgtgttt gaataactga
    28861 cagttctgca ccagcctgaa gaagagcagc tgcagtataa gcaccttcta caattggaac
    28921 cctattgatc atgatacttt tatcactgaa atcagccacc atttctaagt tcattttagc
    28981 agaacctagg tcaaaaaagg ctaataaagt atctgctgga ttttcggaaa caaccctatc
    29041 tacttgatca aaacttgttc caattcctcc atcttcggtt cctcctacat aagtaattgg
    29101 aacatcttta gctacttcac taatcagttc aacaagacct tctgcaatgt ttttggaatg
    29161 tgaaacgata acaagaccaa tactttccat caaaccaccc cagtttctaa aatagcagta
    29221 aagagtaatc ctgatgagaa tgatccagga tcaatatgtc caagagaacg ttctcctaca
    29281 taagatgctc ttcctttagt tgcaagtaaa tctttggtag cattcactac cttatcaata
    29341 agctcatgag tcaattggcc atcagacaag gcagcaatag caggagtcca aacatcaacc
    29401 attgtcttct ctccaactac tgctttctca cgtttgacaa tcatatccaa accactttgt
    29461 aagatttctt ctaattttga atgagaatca gccttggaca ttcccataaa agcagagcca
    29521 tacaagggac cagaagcacc gcctacctta ctcagtagtt gcattgaaac aactttaaac
    29581 acatcactgc ttgtctcaaa ttgctttcct tctaaatttt ccataactgc agccattcca
    29641 cgcgccatat ttccaccgtg gtctccatcc cctataggag tgtctaactc actaaggtaa
    29701 tctttctgtt cttgaatctt ttcattaaaa agcttcatcc attcaagtgc ttgttcagca
    29761 tttatgcgac atttcctcct tgagttttac caagctggtg tagtaacagg tgcatttaga
    29821 gcatccaacc attcatcatt tgccaatcga atcaatgtca atgacaagcc agccatatcg
    29881 attgaggtca tatagttccc aattttctta aatgacaact caacaccttt ttcatggagt
    29941 aatttagcca catcatttgc aaatacgtat tgctccataa gaggggtgct tcctaaacca
    30001 ttgatgagaa gcccatagcg ttcaccagct ttaagttgga aaccttcaga tagtttttca
    30061 accaattctg aggctaattg tgctgaaggt tgcattttct ctttcttata tcctggctca
    30121 ccatgaatac caactccata ttcaaattca tcatcttcca gaataaatcc tggtttccca
    30181 acctcaggaa cggttgctcc agacagggct agcccaattg ttttaatttc taagacaagt
    30241 ttatcggcca agtccttcat ttcagataat gatttaccag aacgagctgc agcccccaaa
    30301 attttatgga ctaatatagt acctgcaaca cctcgacgcc cttgggtata gagactattt
    30361 tcaacagcga tatcatcatc aaccacaacg cttgctacat caataccttc catttcagca
    30421 agttcttgtg caatttcaaa gttcataatg tcaccagaat agttcttgat gaccatgaaa
    30481 actccagcac cttcatcagc cgccttgatg gcttctaaaa tttggtccgg agtaggtgaa
    30541 gtgaagactg ctccacaaat ggcagcagac aacattccat ctcctacaaa tccagcatga
    30601 cttggttcat gtcctgaacc tccacctgaa atgagtccaa cttttcctgt cttttctacc
    30661 tttctaacga tgacatcaaa accatctaag cgttctacca agtcatcatg catgaatgac
    30721 aatccttgca acatttcatc aacaacttgt gtcggtttat ttataatttt tttcatgaga
    30781 aactcctttc ggcatttact tttgttttcg ctttcattat atatttttta ttgctggatg
    30841 ataagggaca gattctatta tttgtcccct tttaaacccg tttaaaacat ttttttggta
    30901 aaatgaagta agtaaaagaa aggcttccta tggcatcatc actcattaca aaaaaacgga
    30961 ttgccaaggc atttagagac ctattagcta ctagagaatt tgacaaaatc tctattgtag
    31021 atatcatgga atcagctggc attcgtagac agacctttta caatcacttt cttgacaaat
    31081 atgaactgct agactggatt tttgaaactg acttaaccga gtatattacc aataacttgg
    31141 acttcatttc gggccaaaaa ctattacaag aattatttct ttatttcgaa caagagcgtg
    31201 acttttatat tcaacttttt gatattcaag ggcaaaataa cttctatgac caatttatca
    31261 gctactgtcg cttgcttgta tcaaaaattt taagagaata tggtcagatt gatatcgatt
    31321 catctgccta tacttgtttt ttgttagact atcattcaca tgctctagca gaaatcgtga
    31381 aggcttacgt caataaaaaa agtccagttc cacaaccaga ctttctaatc atgacaatta
    31441 ttggaaagag accacaatga catttatttc aaatcataaa caaaaaatta tttcaagtta
    31501 catttcagca actgtcagca gtcatcctga attagaaaaa caccctactt taccactagt
    31561 ctatcaaaaa aatcgcaatc ctcatcaggt tccaatattg tcgggtggag gttccggtca
    31621 cgaacctgcg catattggat atgtgggaga aggcatgctt accgctgcta tctatggcca
    31681 attatttact cctcctacaa gaactgaaat cttagagtcc attcgttttt taaacaatgg
    31741 tcacggtgtc ttcatcattg taaaaaattt cgaagcagac atcaaagaat ttagctgggc
    31801 cattaatact gctagacaag aaggaattaa ggtcggctat agtctagcac acgacgatat
    31861 ttctattgaa cctcacaata gatttcaaat tagaggaaga gggcttgcag ggactatttt
    31921 acttcataaa gttctaggat atgcagctca aaatggtgcc gacatcgagc aactaactga
    31981 tttaggtcat gaacttgctc ccgaaatcgc aacaattggc tttgcaacga aatctgctag
    32041 cctccctcaa gccacccttc cactatttaa cttggaagaa ggaaatattt cctatggtat
    32101 tgggatacac ggtgaagaag gttatcgtat tgtcccattt caatcatcgg aaatcctggc
    32161 aaatgaaatt ataagcaaat taagattgca ctatcattgg aaaaagggtg atcaatttat
    32221 attgcttgta aataatctag gcactactag caacctagaa atgggcatat ttatcaatga
    32281 tatccttcaa ctcttagaaa ttgaaggagt cactattacc tttataaaat caggaacatt
    32341 tatgaccagt ctggatatgg caggtatatc cgtaactctt tgccctgtaa aaaataaaca
    32401 atggttaaaa gcgctcaatg ctccaacgac agcttttgca tggtaattct attctataac
    32461 tcaaaagggc tacgtcactt gatagccctt ttaatcttat ctcatgttta aaagaattca
    32521 ttctttttca aagagtccat ctgcatttta caattcttct gatgtgcttt gatctttttt
    32581 agagcccaat catagtgact ggaggtgcta ctgacaaagt aggaacctag acttgttcct
    32641 cccgtccact tatagatacc tttggcaaag agttcgtcat tgctgaagcc ttccatcaac
    32701 tctaaaacct ctctatgtga ttgttctagg agtctagtcg cttcttctaa ggaggttttc
    32761 tggtgctttt tccaaatagt gacattcatc ttcccataag ttttccaatt ataaggttca
    32821 gggagaaaag gtctctcatg ccccttttga ttagaatgta cccaagtcaa aagtaactgc
    32881 tgccattcat agagatggat cagaacatct cttagatttt tatctctttt ccagtgcgct
    32941 tcctttttct tttggtcttt tgaaaaatca aatggagtct gtagctcatc ttcacttagt
    33001 tgggagatga aacggttgag cttgtcatag ttttccttag aggctagcac tagctcctct
    33061 tttgtttttg gtctaggcat aggagtactc ctttcatatc cctagttatt attagagatt
    33121 ttttatcctt taaatctcta caacttatcc tttaatttct ccacaaagag ataggccgct
    33181 ggacaggtca aggtattctt aatcgtcaga tggttgattt gatggatctt tttgcggtca
    33241 gtatgaggga attcacgaca ggcctttgga cgaacatcat agatagagca gagattatct
    33301 cctcctaaaa atgggcaagg catggatttg aaaaccttgt ccccatcttc atctacctgc
    33361 agaaactccg cttcaaaagc tggtaatttc atcttaaaat acttggcaat acgagtaata
    33421 tccgcttctt taaagtcagg tcccaatgtc ttgcaacagt tagcacaggc ggtacaatca
    33481 atctcctcaa agacttcttg gtgaatctgc tgggctatct tgtctaggtt tttaggaggt
    33541 tttttcttta gattggctaa aacttttcga tgctccttct gcttttgcaa ggctagctga
    33601 tggtaatact caatatcaat ttctttagac atggtttctc tcttattcta tttgtttccc
    33661 cctattatat catgcctctg actcattgaa aagtggactt tataagacca tacttttcca
    33721 aaatgcgtca aaaaaacctt gcaactaggt tacaaggtta atgacattaa tcgaaattaa
    33781 cgtattcttt ttgaagttca aggacttctt ccattgttga acactctgta agggcacggt
    33841 tagcgtactc ttccatctta gcagtatcta atttcttcat caagctacgt gtacgaagga
    33901 cagatgttgc tgacatagag aactcatcca agcccattcc gacaagaagt ggaacagctg
    33961 tttggtcacc agccatctca ccacacatac cagcccattt accttcagcg tgagctgcct
    34021 taataacgtt gttaatcaag cgaaggattg atgggttgta tggttggtaa aggtatgaaa
    34081 cttgttcgtt catacggtct gctgccattg tatattggat caagtcgttt gtaccaattg
    34141 agaagaagtc aacttcttta gcaaattggt ctgcaagcat agccgctgca ggaatctcga
    34201 tcatgatacc aacttggatg ttatccgcaa ctgcaacacc ttcagcaaga aggtttgctt
    34261 tttcttcatt aaagactgct ttcgctgcac ggaattcttt caagagcgca accattggga
    34321 acatgatacg caattgaccg tgaacagacg cacgaagaag agcacggatt tgtgtgcgga
    34381 acatagcatc tccagtctca gagatagaga tacgaagagc acggaatcca aggaatgggt
    34441 tcatttcgtg aggcatatcg aagtaaggaa gttccttatc tccaccgata tccattgtac
    34501 gaacgacaac aggtttaccg ttcattcctt caagaacagc cttgtatgct tcatactgct
    34561 catcttcagt tgggaagtct tgagaatcca tgtacaagaa ctctgtacgg taaagtccaa
    34621 cagcttctgc accgttgttg ttaacacctt caacgtcttt tggagtaccg atattagcag
    34681 ccaactcgaa gtgtttaccg tcagcagtca ctgtttgagc atctttcaag agcgcccatt
    34741 cagctttttg tttcgcataa gcttcaccag ctgctttaaa ttctgccgct tgttcatcag
    34801 ttgggttgat aataacctca ccagtgatcc cgttaacagc aaggatgtca ccgtctttaa
    34861 cgatttcagt gatgttgttt gtacctaata cagctgcaat ttcaagtgta cgtgccatga
    34921 tagctgagtg gcttgtacgt ccaccgatgt tggttacaaa agcttttaca aaatttttgt
    34981 ccaattgagc tgtatcagat ggtgtcaagt catgtgcaat cacaatcact tcttcattga
    35041 tagaagctgg gtttggtaat tttttaccaa gaaggtttgc caatacacgt tttgtcacgt
    35101 cgcggatatc cgctgcgcgt tcttgcatgt atgggttgtc ttccatgcct tcaaagatag
    35161 tgataaacat atctgtaact tctttcagac ctgcttctgc attcactttc ttcgcacgga
    35221 tagtttcctt gatttggctg atcatttctg ggtcagcaag aaccattaag tgagcatcaa
    35281 aaacttgagc tgcttcttca ccgagcgtac ctactgcttt ctcgcgaata acagaaagct
    35341 cgtcttgtga tgcctgtaga gcggcatcaa ggcgagcttc ttctgcgttt gtatcttcga
    35401 ctgtaatagt ctcaaatgac aaatccggct gaacgagtag atatgctttt gcaactgcaa
    35461 caccgtcaga tgctgcgatt cctttaagca tttctgtcat ttcccttatg ccaatccttc
    35521 tttttccatt gtttctgaga ttgcagcgat agcgtcacct gcatctgcac cttcagctga
    35581 gatagttacg tcagcacctt gaccaacacc aagactcata acacccataa ttgatttaag
    35641 gttaactgat ttacctttgt actcaagagt gatatctgaa gcaaatttgc tagcagtttg
    35701 taccaacaat gttgctggac gtgcgtgaat acctgtttct gccactacgt ggaaatcttt
    35761 agaagccata gtttgactct ccttttgttc tttctttttt tgagttatat gtgataaccc
    35821 ttacaattta g
//
LOCUS       DAVKDZ010000013        34721 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000013 DAVKDZ010000000
VERSION     DAVKDZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34721
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.013"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(242..460)
                     /locus_tag="VWZ30_000624"
     CDS             complement(242..460)
                     /locus_tag="VWZ30_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEU9334416.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
     gene            612..692
                     /locus_tag="VWZ30_000625"
                     /pseudo
     CDS             612..692
                     /locus_tag="VWZ30_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(1210..1347)
                     /locus_tag="VWZ30_000626"
                     /pseudo
     CDS             complement(1210..1347)
                     /locus_tag="VWZ30_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(1671..2360)
                     /gene="rplA"
                     /locus_tag="VWZ30_000627"
     CDS             complement(1671..2360)
                     /gene="rplA"
                     /locus_tag="VWZ30_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU9334417.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(2568..2993)
                     /gene="rplK"
                     /locus_tag="VWZ30_000628"
     CDS             complement(2568..2993)
                     /gene="rplK"
                     /locus_tag="VWZ30_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU9334418.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            complement(3257..3973)
                     /locus_tag="VWZ30_000629"
     CDS             complement(3257..3973)
                     /locus_tag="VWZ30_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU9334419.1"
                     /translation="MKKRYLVLTALLTLSLAACSQEKAKNEDGEAKTEQTAKTDGTVG
                     SKSQGTAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFSISDHYSGFRSYETQTKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AESGLSLEEYYGFEGGDYVD"
     gene            complement(3976..4446)
                     /locus_tag="VWZ30_000630"
     CDS             complement(3976..4446)
                     /locus_tag="VWZ30_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9334420.1"
                     /translation="MCLICQRIDLIKKEENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVTELHHLKKETRLRFLEEMSLVQEAVAKAFTAEKMNIELLGNGDAHLHWHLFPRR
                     TGDMNGHGLNGRGPVWWVPFEEMTAETCQAKSDEIKQLVKRLSSELDKLLEIKE"
     gene            complement(4448..5485)
                     /locus_tag="VWZ30_000631"
     CDS             complement(4448..5485)
                     /locus_tag="VWZ30_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU9334421.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEEHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVGLA
                     KEQEILFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSGLVD"
     gene            complement(5583..6908)
                     /gene="brnQ"
                     /locus_tag="VWZ30_000632"
     CDS             complement(5583..6908)
                     /gene="brnQ"
                     /locus_tag="VWZ30_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEU9334422.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(7079..7171)
                     /locus_tag="VWZ30_000633"
     CDS             complement(7079..7171)
                     /locus_tag="VWZ30_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9334423.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(7214..7366)
                     /locus_tag="VWZ30_000634"
     CDS             complement(7214..7366)
                     /locus_tag="VWZ30_000634"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9334424.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            complement(7526..8104)
                     /locus_tag="VWZ30_000635"
     CDS             complement(7526..8104)
                     /locus_tag="VWZ30_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU9334425.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKYYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(8158..9558)
                     /gene="pepV"
                     /locus_tag="VWZ30_000636"
     CDS             complement(8158..9558)
                     /gene="pepV"
                     /locus_tag="VWZ30_000636"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEU9334426.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(9571..10176)
                     /locus_tag="VWZ30_000637"
     CDS             complement(9571..10176)
                     /locus_tag="VWZ30_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU9334427.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYSEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            10347..11147
                     /locus_tag="VWZ30_000638"
     CDS             10347..11147
                     /locus_tag="VWZ30_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9334428.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRDLDLSVVDLPSVDSPNN
                     YIVFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(11215..12048)
                     /locus_tag="VWZ30_000639"
     CDS             complement(11215..12048)
                     /locus_tag="VWZ30_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9334429.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEVLDGEYGLEYPKEIQSMRMIQFLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPNKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNIQFRKV
                     AYDYEAELELAKSKRLPFIEMYEELRREDNYQGHNLELLASLIEKHGYVEDVKDYFDF
                     L"
     gene            complement(12038..13882)
                     /gene="uvrC"
                     /locus_tag="VWZ30_000640"
     CDS             complement(12038..13882)
                     /gene="uvrC"
                     /locus_tag="VWZ30_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU9334430.1"
                     /translation="MNNLIKPKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVRAANEIKRLLDRIFPFRKCTNLPSKVCF
                     YYHIGQCMAHTICKKDEVYFKSMAQEVSDFLKGQDDKIIDNLKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDQEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVAGPDDYASMREVIRRRYGRVQRDGLTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEVVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            complement(13921..14610)
                     /locus_tag="VWZ30_000641"
                     /pseudo
     CDS             complement(13921..14610)
                     /locus_tag="VWZ30_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
     gene            complement(14689..15921)
                     /gene="murN"
                     /locus_tag="VWZ30_000642"
     CDS             complement(14689..15921)
                     /gene="murN"
                     /locus_tag="VWZ30_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEU9334431.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     IKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(15926..17146)
                     /gene="murM"
                     /locus_tag="VWZ30_000643"
     CDS             complement(15926..17146)
                     /gene="murM"
                     /locus_tag="VWZ30_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEU9334432.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDAGQARVPLAATLTLEFGQTSVNLYAGMDEDFRRYKAPILTWY
                     ETARYSFERGILWQNLGGVENNLDGGLYRFKANFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(17258..18037)
                     /locus_tag="VWZ30_000644"
     CDS             complement(17258..18037)
                     /locus_tag="VWZ30_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9334433.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNVTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQDFSPESQNLG
                     TPAYWRGVFGEIKDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEGFLTTLPIDFKLEERLT"
     gene            complement(18106..19767)
                     /locus_tag="VWZ30_000645"
     CDS             complement(18106..19767)
                     /locus_tag="VWZ30_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9334434.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(19942..22164)
                     /gene="recJ"
                     /locus_tag="VWZ30_000646"
     CDS             complement(19942..22164)
                     /gene="recJ"
                     /locus_tag="VWZ30_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU9334435.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEEM
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPYRDLFIAFGGHAGAAGMTLEVEQLSDLSQILEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQYFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYRFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESHIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            22440..23108
                     /locus_tag="VWZ30_000647"
                     /pseudo
     CDS             22440..23108
                     /locus_tag="VWZ30_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            23106..23792
                     /locus_tag="VWZ30_000648"
                     /pseudo
     CDS             23106..23792
                     /locus_tag="VWZ30_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726191.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
     gene            23805..24482
                     /locus_tag="VWZ30_000649"
     CDS             23805..24482
                     /locus_tag="VWZ30_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9334436.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYSLPLISDHTIMIPIYWTAVLCVGL
                     YHGAYTAEVIRSGIQSIPSGQMEVALSQGFTYISAMRLIILPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGVALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            24492..25151
                     /locus_tag="VWZ30_000650"
     CDS             24492..25151
                     /locus_tag="VWZ30_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9334437.1"
                     /translation="MESILEVLSPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPILRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            25445..26635
                     /locus_tag="VWZ30_000651"
     CDS             25445..26635
                     /locus_tag="VWZ30_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU9334438.1"
                     /translation="MKSIKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTAHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FVSIILVAYLGKYIQYEAWRWIHRMVYLAYIFGLFHVYMMMGNRLLTFNLLSFLVGSS
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQAGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQVGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYNFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(26955..27836)
                     /locus_tag="VWZ30_000652"
     CDS             complement(26955..27836)
                     /locus_tag="VWZ30_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEU9334439.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(27977..29305)
                     /gene="vncS"
                     /locus_tag="VWZ30_000653"
     CDS             complement(27977..29305)
                     /gene="vncS"
                     /locus_tag="VWZ30_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEU9334440.1"
                     /translation="MKRTGLFTKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTKNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQFF
                     SGNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVAQD"
     gene            complement(29302..29958)
                     /gene="vncR"
                     /locus_tag="VWZ30_000654"
     CDS             complement(29302..29958)
                     /gene="vncR"
                     /locus_tag="VWZ30_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEU9334441.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(30007..30090)
                     /gene="pep27"
                     /locus_tag="VWZ30_000655"
     CDS             complement(30007..30090)
                     /gene="pep27"
                     /locus_tag="VWZ30_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEU9334442.1"
                     /translation="MRKEFHNVLSSGQLLTDKRPARDYNRK"
     gene            complement(30237..31616)
                     /gene="vex3"
                     /locus_tag="VWZ30_000656"
     CDS             complement(30237..31616)
                     /gene="vex3"
                     /locus_tag="VWZ30_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEU9334443.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSFKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIDTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AVQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIVFVLALVLVVAVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(31668..32315)
                     /gene="vex2"
                     /locus_tag="VWZ30_000657"
     CDS             complement(31668..32315)
                     /gene="vex2"
                     /locus_tag="VWZ30_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEU9334444.1"
                     /translation="MTLLQLQDLTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(32442..33719)
                     /locus_tag="VWZ30_000658"
     CDS             complement(32442..33719)
                     /locus_tag="VWZ30_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9334445.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VETNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVS
                     GEQSVERDDLSAADKNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHGKIALDAGQSEAGKGQTVEFEIVGIFSGKKQEKFTGLSSDFSENQVFT
                     DYDSSQTLLGNSEPQVSAARFYVENPKEMDGLVKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKTSIFLQF
                     CLEVVLVSLGALLPAFVVGNAITSYLLQTLLASGEQASLQDTLAKASSLSTNILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            complement(33870..34427)
                     /locus_tag="VWZ30_000659"
                     /pseudo
     CDS             complement(33870..34427)
                     /locus_tag="VWZ30_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035094.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         9830 a         7885 c         6501 g        10505 t
ORIGIN      
        1 agattcagtc tactatacat aaactaacct cctcattatt agcaaggctt ctactgatta
       61 taacctaatt cttagactac ttcaagacta actgtccaat aataatctac agataaatca
      121 tccactctta atcaaatttt attcaattca tctactcatt cccaaaaggt tgctgtataa
      181 tgcaagacat ttttcttact gcttttactt ttccttaccc tcaatttagt gagctaataa
      241 attattcttc ttcaaaaggt tgagatttat ctagtaactc taaaacatta atggtaggta
      301 gcaacattgc ttcactgcta tcaattgccg aaactattga aacaatagaa cgtaaataag
      361 gataaagaat agcagtacca tttacgtata gaatatcggt gatattttca tctatattta
      421 tttcaaaata tcctattaca gttacagata tttttttcat ggcatctttg tcaactaaag
      481 aaacactaag ttttacggct ccatgactat aatcatctgt atgaccaacc ttaactatgg
      541 gatataatat atcgaaggta aatgtgaaca aactgattag gaaatttaaa ccaacttcta
      601 acaatgtgtt atactcaatg aaaaccaaag agcaaactag gaaactagcc gcaggttgct
      661 caaaacactg ttttgaagtt atggatagaa ctgacgtagt cagctcaaaa cactgttttg
      721 aggttgtgga tagaactgac gtagtcagct caaaacactg ttttgaggtt gtggatagaa
      781 ctgacgaagt cagctcaaaa cactgttttg aggttgtgga tagaactgac gtagtcagct
      841 caaaacactg ttttgagatt gtggatagaa ctgacgtagt cagctcaaaa cactgttttg
      901 aggttgtgga tagaactgac gtagtcagct caaaacactg ttttgaggtt gtggatagaa
      961 ctgacgtagt cagctcaaaa cactgttttg aggttgtgga tagaactgac gtagtcagta
     1021 accatacgca cggcaaggcg acgttgacgt ggtttgaaga gattttcgaa gagtattaga
     1081 aagctcgatg gactattcca gattcattcc actagatttc gaaatcgagt aggggataat
     1141 ctctagcccc tctcacacca ccgtacgtgc cgttcggcat acggcggttc aactaactgt
     1201 taacgcatgt cattcaaggt aataatccaa acacgaaacc agtccacgtt tttcaaggac
     1261 tggttttgat atagcacgtt taagtaccga cttctgagct actaattgat agtggtcgcc
     1321 ccagccagat accttatccg ctatataggc cttattatca cgttcctgtt cgtagagcca
     1381 cgatttcgct atcccttcct ctgacgattc ttatttccag cgtcagactt gggaatcgct
     1441 attgggttca ccggtagcag gtgcccaccg aggacttaca cctcaaatat ataataaaat
     1501 gaaataagaa taggacgaat cgatcaggac agtcaaatca atttctaaca atgttttaga
     1561 agtagatgtg tactattcta gtttcaatct actatacaac atgcccgtcg tacatcaaaa
     1621 aaagttgaga catagcgtct tcaacttttt tacattactt taactactga ttaaagtgag
     1681 tttacgtcaa ctttgatacc aacaccttga gtagttgtga tagtcaagtt tgttacgtaa
     1741 gttcctttag ctgtagctgg ttttgctttt tggattgttt cgttgaaagc tttaaagttt
     1801 tcaaccaatt tttcagcttc aaatgatact ttaccgatga ttgcttgaac gttacctgca
     1861 cggtcagcac ggtaagtgat tttaccacct ttagactctt caactgcttt agcaacatcc
     1921 attgttacag taccagtttt agggtttggc atcaagttac gtggtccaag gacacgtcca
     1981 agacgtccaa caagagccat catatcaggt gtagcgataa ctacgtcgaa gtccaaccaa
     2041 ccgtcgttga ttttagcaac aaggtcatct tcaccaacaa agtctgcacc agcagctttt
     2101 gcttcttcag cttttgcacc acgtgcgaaa acaagaacac gtgaagtttt accagtaccg
     2161 tttggcaata ccattgctcc acggatttgt tggtcagctt ttttaacgtc gatgttcaag
     2221 ttgtaagcaa cttctacagt tgcgtcaaat tttgcaaagt tagtttcttt tgcaagtgct
     2281 acagcttctt ctacactgta tgcttttgtg ctgtcgattt tctcaagagc agcacgaagt
     2341 tgtttgcttt ttttagccat tttctattct ccttgtaagt ggttcaatcg attttcatct
     2401 cccacgtcac ttctcgaaat gatgaagtct tgcgggtttt cagtctacta tttttctctt
     2461 cctacggcct taagctccct tgctgtaccc aagtacaagg ctcagtcact tgcctagtat
     2521 gaaaagcaac taatagactg ctgtgagatt ttctactgtg ttattgatta gtcaacaaca
     2581 gtgaatccca tagaacgagc agtaccttcg atcatacgca ttgcagactc tacgtttgct
     2641 gcgttcaaat ctggcatctt agtttctgca atttcttgta cttgtgcacg agtaactgta
     2701 gcaactttag ttttattagg tgtacctgat cctttttcaa cacctgcagc ttttttcaaa
     2761 agaacagcag ctggtggtgt tttcgtaacg aaagtaaatg atttatcttc gtaaactgag
     2821 ataacaactg gaatgatcat accagcttgg tcagctgtac gagcgttgaa ctcttttgtg
     2881 aatcccatga tgttgatacc agcttgacca agagcaggtc caaccggtgg agctggtgta
     2941 gctttaccag cagggatttg caattttaca agtttttcga cttttttagc catttctaaa
     3001 tcctcctttg tggttttggc ggtaatgtaa gatttttacc tcccacaagt atgtttttca
     3061 catacccatc cattatacac tatttatctg gaattgcaag agaaaagttt attttttata
     3121 ctcaatgaaa atcaaagagc aaactaggaa gctagccgca ggctgctcaa aacactgttt
     3181 tgaggttgtg aatagaaccg acgcagtcag taggtaaggc gacgctgacg tggtctgaag
     3241 agattttcga agagtattaa tcgacgtagt ctccgccttc aaagccatag tattcttcca
     3301 aactgagacc actctcagca atttcttttg cttcttttcc gacataacgc aagtgccatt
     3361 cctcagccat atagcctgtt tccttttcct tgcctttgag ataacggaca acaaagccat
     3421 aatcagcagc atggtccaag agccattggg ctgctttttc ttctgtcacc aaatcaccat
     3481 cagtcccaat cacatcaaag gccaagcctg tttggtgttc gctatagcca ggtcgagcag
     3541 aataacggtc agcagcttct ttcccatctt gattgacata atcttggtag agcttggtct
     3601 gagtttcata acttctaaag ccactgtaat ggtcactgat tgagaaacct gcctcttgca
     3661 tcgctttgat gagtttaacc aactcagcct tggctgttgg attttcccct ggattatagt
     3721 ctttagacaa tggatagtgt ttgttggcta cgatgatttc atcgtatttc ccttgaatgc
     3781 tgtaatagtc tcctttgtta accacttctg ctttcttctg ggcagttcct tgagacttac
     3841 ttccgactgt tccatcagtt ttggctgtct gttctgtctt agcttctccg tcttcatttt
     3901 ttgctttttc ttgtgaacaa gctgctagac tcaaggttag caaggctgtc aagacaaggt
     3961 atcttttttt catttctact cctttatttc taatagttta tctagttctg acgataaacg
     4021 tttgactaat tgtttaatct catccgattt tgcttggcag gtttctgctg tcatttcttc
     4081 aaaaggaacc caccagactg gaccacgtcc attgagaccg tgaccattca tgtcacctgt
     4141 tcgtctagga aacagatgcc aatgaagatg ggcatcgcca tttcctagca gttcgatatt
     4201 cattttctca gcagtaaagg ccttagcaac tgcctcttgg actagactca tttcttcgag
     4261 aaaacggagt cttgtttcct ttttcaaatg gtgcaattcg gtaacgtgtt ccttagctag
     4321 aaaaagacta tatcctgcaa aatactggtg gtctccaatc acaagatagc ctgtttccaa
     4381 ctctttgaca aagtaaggat tttcttcctt cttgatgagg tcaattctct ggcaaatcag
     4441 gcacatatta gtctaccaac ccactcttaa gataagcatc aaccatacgc tctgttgcga
     4501 ccaccgagtc aatatgagta cgctcataag aatgactaga ctcgataccc gcaccaagaa
     4561 gagcgtgttt aacttctgca cctgcagaca tagccgctga agcgtccgat ccataaaatg
     4621 gatagatatc caacttaaat agaatttctt gttctttagc caatcccacc aagtgttgac
     4681 ggaagtcata gtgataagga cctgaagcat ccttgacaca gatagatact gtgtactcat
     4741 ctgtctgctg gtcatctccc atagctccca tatccacagc cagatattct actacctgag
     4801 caggaatgtt agaattagca ccatgtccta cttcttcaaa aactgaaaaa gcaaaatgag
     4861 ttgttactgg caattcaatc ttctctttct tataaatacg aagcaggttg agcaaaatcg
     4921 ctgcgctgac cttatcatcc aaatgacgag acttgataaa acctgtatca gtcacaacag
     4981 ttcgtggatc gaagctgata aaatcaccga cctcaatccc aagagcacga gtttcttttt
     5041 cattgcttac ttttacatcc aaacgcactt ccatattgtc ctgcgtgcgc tctgcagttc
     5101 ctgcatcctt gtagacatgg caagaagttt ggtggatgag gatggttcct gatacctttt
     5161 gacctgtgct agccacatga acggtacagt tttcaccttc aatcatgttc caaggaaaac
     5221 caccgatacg gtccaattta agacgaccat ctggtttgac agcacgaaca atagcaccca
     5281 gcgtatccac atgagcagtc acatagcgat gctcttcatc attttgacct ttgatggtca
     5341 cattgacacc acccttagct gtgcgaactg gctggtaacc aaaaccttct agagtcttga
     5401 ctaaataatt cgaaatttca cgagtaaagc ccgttggcga tgcgatggct gtcagttctt
     5461 tgatatattc tactgtttga ttcatttctt cacctctttc gctacctatt ataacacatt
     5521 tatcatcgga taaaaaaaga aaatcactcc tgtagacttg gctacaaaag tgatttaagt
     5581 gattattcca tttcaaaaac atcgctttct tgcttgtttg gtagaaccaa tgagagcaag
     5641 attccaacaa tggctggcac caaccatggg agagatgcct tggcaaaagg aagaacgcta
     5701 acaagatttg caagaaactc aaccttaaac gagcttccta gtacacttgc aatggcaata
     5761 actgtaacca cagcaattgt caactgcata cctggttttg aaagggcaac aaatttgttg
     5821 acaatgacaa tcataacgat agcaatcgtg attgggtaca agataaccag tactggaatt
     5881 gagtacttga taatcgcatc aagacccaaa ttggcaatag caaatccaat caaggtaaag
     5941 gctgtcgcat aaaccttgta gctgatttgt gggaagcgct cattaaagaa ctcagctgtt
     6001 gacacaatca aaccaacagt cgttgtgaag caggttacgg taaccatagc tgcaaggaag
     6061 agttgagctg ttgagccaaa gatttcttga gtggcttgtg acaagatgta aacacctggt
     6121 gttccaccct tcatcgcttc agctggtact gggaaatgat ttccaagaaa acctaaaccg
     6181 atgtaaagag cgctgaaggc aagggcaaca acgataccaa caacccaaat agttgaaatg
     6241 tattctttct tacttgaaaa tccaagttgt ttcaaggttt gaactgcgat tacgctaaag
     6301 gccactgagg caagggcgtc caaggtatta taaccttcta ggaaacctgt accaaaggca
     6361 gaagcttgat aagcagctga agcagcttga ggacttgttc caccatattt gatagctccc
     6421 agaacgacca agataacaat caaaattgca aagactggcg ttaaaatacg tccaatgcgg
     6481 tctaagattt ttgatggatt aagcgaaatc aaataggctg ccgcaaaata cagaaccgta
     6541 aatacaatca agccaagtcc tttatttgca tccgacaaaa gggggctaat ccctacttcg
     6601 taagctgttg tagcagtacg tggggtagca aagaatggac cgattgacaa gtaaagaact
     6661 gagaggtaaa gagtcgcaaa ccaaggcgct atcttcgttg aaatctcgta gatatatcct
     6721 ttaggattta gcgttccaat aataagggtc aagacggcga taccaacgcc tgaaaagaca
     6781 aaacctgcga tggcaggaag aaaatgttct ccagatagag cacctagaga aggcggaaaa
     6841 atcaagttcc ccgcaccaaa aaatattcca aacaggagca aacctgttag ggtacctttt
     6901 ttagccataa aaatctcctt catatttata aaatactgac ctagtatacc acgattagga
     6961 gtctgaaaaa agcatcacga ggcaaatatt aaatgttttc aattttctta aaaataagaa
     7021 ttatctaaaa ataaaactct cctacccagt ccacaccaag taggagaaat gcaaaatttt
     7081 aaagttcttc aaaggctaac atgccaccct ggacattgat aacattataa ccttgttcta
     7141 ataggaattg gcaagcacgc gccgatctca ttccagattt gcaaataata taatgcaagc
     7201 gatctttgtc caattaatca taactatcag ccaattgact aagcggtagg ttgtgggcac
     7261 cttctaaatg aagagctgca aactcatcca cttctctcac atccactaga gaaagctggt
     7321 cgttttggta aagctggtaa aatgcgtcaa aggctatttc tttcattctt tttcttctaa
     7381 tactcttcga aaatctcttc aaaccacgtc agcgtcgcct taccgtgcgt atgttaccga
     7441 cttcgtcagt tttatctgca acttcaaagc tgtactttga gcagcctacg actagcttcc
     7501 tagtttgctc tttgattttc attgactata aaatggtttt aattcttttt ttcaaatctg
     7561 gcactacttc tgcctcaaac caaggatttt tggccatcca gatttgattt cgtggtgatg
     7621 ggtgaactag cggaaaatag gctggcagat agtctttata gtatttcacc ctctccgtta
     7681 ccttcccact gattttctcc tgtaaatagt aggcttgggc atattgccca atcaagaggg
     7741 ttaactgaat atcaggcaat tcctgtaaga gctgcggatg ccatttttct gcaaaacctg
     7801 tacgaggcgg aagatcaccc gacttgccat gtcctggaaa gtagaaatcc ataggcaaaa
     7861 cagcaaaata acctgaattg taaaaggtat cttcatccac acctagccag tcccgcaagc
     7921 ggtcaccact tttatctttc cagtaaaggc ctgcttcttg agttttaagt cccggagcct
     7981 gaccgatgat attgatgcga gcagtttttg gcgctgcaaa gagaggctca atgccacgct
     8041 ctgtatagct ggcattctgt gaatccgcca tgatagcctg tttgattctt tcgatttgag
     8101 acatctactt tccctccaaa aagaagtcca agcataagat ctcagacttc tatcgtttta
     8161 tttgatcaat tcgtaaatag cttcggcata aattgctgct gctcggaaaa gatcatccaa
     8221 ggcgataaat tcattggctt ggtgcatggt atcaatcgag tctgggaaca tagcaccgta
     8281 ggcaactccg cgttctagca agcgaccaaa ggttccacca ccgatgactt gttcatgacc
     8341 tttaaggcca gtttgttttt catagacatt caacaaggtt tgcacaagtg gatcttccat
     8401 tggcacatag tgaggcgtgt gaccgtgttc agacaggcta acagaaacaa ctggcaagtt
     8461 ttcaaggatt gacttgattt gttctggact tgttcctttt ggatagcgga tgttgagggc
     8521 aatggtatta tcagcacttg tttcatcgaa gcggaagacg ccggcattca tagaaagagc
     8581 acccatcttt tcatccacat gagcaatctt aagattttca ccctcatgat cgttcaagag
     8641 aattttacct gcgatgtcaa ggtagtcttt ggctggacca gcaaagccaa actggctgag
     8701 gaagagggca aggtaagttg cgccattgac acctgaagca ggcatagcac cgtgggctga
     8761 tttaccaatg atcgtcacct tgtatttgcc agcttcttct tggagttctc ctctaagttt
     8821 gtgttctgca acaaaggcat ctagtttagc ttgcaagtca gccaagtcac ctgaaacgac
     8881 tgctgttgct gattctggta ccatattttc acgtaaacca cctgtaaagc tgtgaagacg
     8941 ggcaacacct gtattttctc ctgcaaagtg gaggtattcc gtgatatttc ctttttcacc
     9001 attgatgatt ggaaattcag catctggtga gaaaccgaaa tctggtttgg caagtcctac
     9061 gtgctcaaag tagtagtcca tgtctgccca gcctgattct tcgtctgttc caacgatgaa
     9121 gcgaactttc ttagaagttg gaagacccaa ttctttgatg attttcaaac catagtaaca
     9181 agctgttgta ggacccttat cgtccgaagc cccgcgcgca taaaggcgac catctttgat
     9241 agttggtgtg taagggtctg tgtcccaacc gctaccagca ggcaccacat ccatatgggc
     9301 aaagattccg agaacttctt ctccatcacc aaactcaaaa tgtcctgcat agttatcaac
     9361 attcttagtt gggtagccat cgcggtctgc gatttcaagg aatttctcca aggcttttac
     9421 tggaccaggc ccaaatggat gctgggcatc agccttgctg tcatcacgtt ctgaattgat
     9481 ttccaaaagg ctaaacaagt cagccaagag gtcttctttg cgtttttcta cttctgctgt
     9541 aaaatcaatt gctgtcattt tttcttcttt ctatcttttc tcgatgattt catctactgg
     9601 caagcggtag cttggttcca atttttcgtc tgtataaccc actgtgatca agagttctgg
     9661 gcggaaacgg tcttcgattt ccaaaacttc attaactttt gatttgtcaa aaccaagaat
     9721 aatgttagaa ccaattcctt ggtctgtcaa tgcaagaacc aagttcatgg caaccaaacc
     9781 tgcattgaga gctaggtagt cgctgacttg ttgctcactg taacgggcaa actcagctgg
     9841 cagatttttc ataaaatatt gaagttgctc ttcagaaaag ttattagcac caccaacacg
     9901 ggcaatctta cgagcacgtt tggctaagtc cgtatctgta aacaaggcaa tggttacagg
     9961 cgctgatgat acctgttcaa aattggaacc ataagctaac tttgccagtt cagcattttt
    10021 ctcacgtacc accacaaatt tccaaggctg gctgttgtgg gcgcttggcg ccaaggttgc
    10081 aatttcgata gccgtacgca catctttggg atcaacaggc ttatcagtaa aatgcttagt
    10141 cgcatgacgt tttttattta attcaagaaa tttcataatc gatttccttt tctaattcta
    10201 ctgcttccat tctaccataa tttgaaagag cttgcaggga tttttcatga ttaagttttt
    10261 ctatcacaga cataaaagat atatattggt ttatcaggaa agtttctgat aaactagcaa
    10321 gtactttaca tttgaatgga gacagtatga aaaaatttag cctattacta gctatcctac
    10381 catttttggt tgcctgtggg aatcaagcta cacccaaaga gactagcgct caaaagacaa
    10441 tcgtccttgc tacagctggc gacgtgccac catttgacta cgaagacaag ggcaatctga
    10501 caggcttcga tatcgaagtt ttaaaggcag tagatgaaaa actcagcgac tacgagattc
    10561 aattccaaag aaccgcctgg gagagcatct tcccaggact tgattctggt cactatcagg
    10621 ctgcggccaa taacttgagt tacacaaaag agcgtgctga aaaatacctt tactcgcttc
    10681 caatttccaa caatcccctc gtccttgtca gcaacaagaa aaatcctttg acttctcttg
    10741 accagatcgc tggtaaaaca acacaagagg ataccggaac ttctaacgct caattcatca
    10801 ataactggaa tcagaaacac actgataatc ccgctacaat taatttttct ggtgaggata
    10861 ttggtaaacg aatcctagac cttgctaacg gagagtttga tttcctagtt tttgacaagg
    10921 tatccgttca aaagatcatc aaggaccgcg acctagatct ctcagttgtt gatttacctt
    10981 ctgtcgatag ccccaacaat tatattgttt tctcaagcga ccaaaaagag tttaaagaac
    11041 aatttgataa agcactcaaa gaactctacc aagacggaac actcgaaaaa ctcagcaata
    11101 cctatctagg tggttcttat ctcccagatc aatctcagtt acaataagat atattaaaag
    11161 cgattgggcc agccaatcgc ttttttagtt tgcattggtt attttaggaa acttttacaa
    11221 aaaatcaaaa taatctttca catcctctac atacccatgc ttttctatta agctggctaa
    11281 aagttccaga ttgtgtccct gatagttatc ttcacgacgt agttcttcat acatttcgat
    11341 aaagggaagc ctcttggact tggccaattc taactcagcc tcgtaatcat aagcaacttt
    11401 acgaaattgg atattgacca attccccgtc ttcaacttct atcacggcat actgggcacg
    11461 gtgatttttt aacgcctccc aattaaaata gggcatgcca atcgaccctg gattgatgat
    11521 ttgttgccct tgacttccat aacgaagcaa ctgcttgtga acatgaccat aaactgccac
    11581 gtccgtttcc gcatctagca gttggtcaaa tttctctgta tcattctcaa ctagcaagtc
    11641 accaccatag ttcttatttg gcaaattatg agagatagaa aagcgcaatc cgtcaatttc
    11701 tttcttttcc agcaaaggca agcttcgtag ccagacaatc gttgcaggat ccattcgctc
    11761 catcaaaaac tggatcatgc gcattgactg gatttctttc ggatattcca aaccatattc
    11821 gccatccaag acctcaagga cacgatcatc ccaattgcct cgaacactgg ctgtgatagg
    11881 aaggtccttt agcagggcga ctaagtcatt tgcgcctgga ccaggaagaa aaatatctcc
    11941 cagaagccaa tattcactgg ccccttgatt tttagcatct gcaatcactg cttctagcgc
    12001 cgtcgcattg ccatgaacat ctgataaaat tgcgatttta tggttcattg tggtttcctt
    12061 ccgtttggta atctactctt tcttctgcta cttgaggcaa ggcttctccc tgcgggttca
    12121 actttctttg cacaacctct gctactgctc ttggcactcc aacttcgaca atctcatcca
    12181 cactggcttc cttgatttta gtcaaagact tgaaatgctt catgagattc tgcttgcgtt
    12241 taggtcccag accgtcaatc ccatccaatt gagatgaaaa ggaatttttg gagcgcagtt
    12301 ggcggtgaaa ggtgatggca aagcggtgca cctcatcttg aatgcgttga aggaggaaaa
    12361 attcttgaga attacgagac aagtccacca cttccagcgg atctccaaag agcaattcat
    12421 gagtttggtg cttatcattc ttttgcagac ctgcaattgg aatatccaag cccagttcct
    12481 cttggataac ctgcttagcg atattgactt gaccttgtcc cccatcaatg acaatcaaat
    12541 ctggaggagt caaaccgtca cgctgtactc gaccatagcg cctgcgaatg acttctctca
    12601 tactggcata gtcgtctggc ccggctacgg tcttgatttt atacttacga taatcttttt
    12661 tacttggttt gccgttgaca aagaccacca tggctgaaac aggactggtc cccatgatgt
    12721 tggagttatc gaaagattcg atacgaactg gggttgggat ttgaagcaag cgtcctagat
    12781 tttcaatagc cccttgggtc ttttcgacag atttctctag caggttgaat ttctgctcta
    12841 ggctgactcg agcattcttt atagccaaat tgaccagctg ctttttctct ccacgctggg
    12901 gcttgagaat cttggtatct accaaagcct tgaccgcttc ttggtcaata tcctgcggaa
    12961 tcagtacctc attgggaact agatgagatt tttcttgata gaattgtcct acataggtca
    13021 aaaaatcctc atctggatca ttgaagtagg ggaatagatt gacatcgcgc tcgatgagct
    13081 tgccctgacg aacaaagaaa acttgcacac acatccagcc cttatccaca tagtagccaa
    13141 agacatcgcg attttggaga tccttagcca tgacccgttg cttggttcga agcgttccaa
    13201 tagcctgaat caggtcacgg tattccgccg cacgttcaaa ctccatagtc tgggccgccg
    13261 ctgccatttt gcccttgagg ttatcaatga ttttgtcatc ctgccctttt aaaaagtcag
    13321 aaacctcctg agccatagat ttgaaataga cctcatcttt cttacagata gtatgggcca
    13381 tgcattggcc gatatggtag taaaaacaaa ccttagaagg aagattggta cacttacgaa
    13441 aaggaaaaat ccgatccagt agtcgcttga tttcattggc cgccctcaca tctggataag
    13501 gtccaaagta gagacctccg tccttcttga cctgacgagt gataatcaaa cgaggatagc
    13561 gctcattagt gattttgatg aagggatagg acttatcatc cttgagcatg atattgtatt
    13621 taggtttatt ttccttgatc aggttgattt ctaggagaag tgcctcaata ttagactcag
    13681 taacgataaa ttcaaaatct acaatttcag acaccaaagc ctctgttttg gtatcatgac
    13741 ttccacggaa ataggaccgc acgcggttac gcagattttt agcctttcct acatagataa
    13801 tggtgccgtt tttatccttg tgaatgtagc aaccagggct ggtcggcaag agctctagtt
    13861 ttggtttaat caagttgttc atagttccat tatagcaaaa aagtagggaa agatagtacc
    13921 ctactcacta gtctcatata tttttcggag gtttgcaaca tcctcttcct ggataccata
    13981 aaaggaacct gctggttgca tgacagactt ctcatctgta tggtccaagc caatcgcatg
    14041 acctaactca tgttctgccg tatggacaag gcgttcatag gaatagccat agtatggatt
    14101 ggacaaataa taatgattca accgcaccgt tacggacaag aattgccctg ttaagagatt
    14161 agtttgactt tctgcctctc ctgccacagg agtgcctccg tcgttcatct ccgtagccgt
    14221 aatatccgcc ttactagact cagtcacgag ttcaaagtta aaagcaccaa tttgattcca
    14281 gttctggatt gcttctaaat aagcctcttg aaagcgtgaa tccatctggg gatccaggta
    14341 aatacgagca gaggcctgtg gccaccttcc ttcagaatgc tggtgactat ctgtctggct
    14401 taacttaggc agttgccctg ttttctctca ttggtcaata ctttgttgac caatttttac
    14461 tgaccgttct gcttgcttta gcgctccttg aaaatccccg ttcagatacc agagaagtcc
    14521 gaaagcaagc acacataaga gcacaactat ccaaaacaaa cgccacacaa aagaaaagaa
    14581 agcccctatc aaacgaaaaa gccagcgcat gtctctccac ctttctagca aataagaatt
    14641 attttactaa aagaagctaa aagaaatcta aatactgact ttttcatctt aacgtcctac
    14701 tatttttttg agtaactgga tagctttgta ttttaaaggc gatggatggt aacggaaagt
    14761 acctgctttg cgtacaatat agccattaaa attctgttta aaacgcaaaa caccatcact
    14821 tccatcaaaa atcccttgaa tgcctaggaa gttgtattta ggtattccac gttttatgct
    14881 ttccaacata acatattttt gaagcagtgc aggggcatag aacttattaa actcagtgta
    14941 ggaaccacta aagagataag tcgtttcctg aggcatataa acaaataaac tcccagctaa
    15001 aacaatatct tcttctccat atttttcaat caagtctcgc gcttctgctt ttcgaacttc
    15061 aaacgtttca aattgactag aatattctct cagttgattt tgttttttct cagaatgagg
    15121 atttttactc aaatcaagtc gcaacttgtc caagttttct tctagtttac tttgttcacc
    15181 ttgcaatttg ctcatatact ccgaaaaatt taagcttgct atgagaaact ccgcttgttc
    15241 tccaaaagta tcataaaaat gctcataata ttctaaactt ttatcactat attctctacg
    15301 ttcagaggtt tcttttgtta tattcttaaa aatcgatagt tcttcacgtt ttaacttttt
    15361 caaccgaatg ccaaaggttt cagccttttt caccaagggt ttaccctttt tgctaaaact
    15421 tttaagcaaa ctcttttcag ttaattcagt taaatcttta tagtataacc aatctggttc
    15481 tccacctggg taacctgttg ttaagccatc aaattgataa cctaaatcag tcaaaccttg
    15541 aataatactt tttttctcag catctattgg attaccttgg ctatcaaaag tttgataagt
    15601 ttcataaggt tttataagca actctaatac accattttgc ttggcatatt cttttaactc
    15661 tgcataaaaa actggaagag catcttgttg ggtataaatc ggccccgaat tgagttccat
    15721 atgcagacca cccagcatgg gcaagctata aaccagagct gcaacttgaa tttctccttc
    15781 ttgtttcaaa gcaagataaa caattcgagc ccctcttttt tctagcaaat cccccatctg
    15841 gacagattgc ataaaggaac gagaagaaac ctgatcagaa taagtctgaa actcttcttt
    15901 cgtgagtgtt gttagtgcca tatacttact ttctatgttt ttttcttaat gttttacgga
    15961 aatcaagagc aagtcttaac agaggataga gaggatgagt gggcattgta aattcaccca
    16021 agtattcttc aatcgttgga ttaaaattag ctttaaaacg ataaagtcca ccatctaaat
    16081 tattttcgac tcctcctaaa ttttgccata aaatgccacg ttcaaaggaa tatctagctg
    16141 tctcatacca ggtcaaaata ggggctttat atcgtctaaa atcttcatcc attcctgcat
    16201 atagattgac agaagtttgt ccgaattcta aagtcaaggt agccgctaaa ggaactctcg
    16261 cttgacctgc atctatatat tcctgcaaga aggtcaattc ctctaacaaa cgttcttttt
    16321 ccttcttctg cgcttctact tttgaagttc gagtcgattc agtaaaggtc tcttccaagg
    16381 ctctgttttt cgctaactgt tcttctaatt cttgcgaacg tttagagaca tccaaggttg
    16441 ccaaggtgat ataggccttg tccttaaaat tatctaacaa ttttttataa taggcttcat
    16501 tcctcaaatg aatctctttt cgcttctcag tttttttcat caactccgaa aatgaatcta
    16561 atagttccag tccaccatat tgaatctcaa gccctttgtt tcgtgctgtt cgaatagcct
    16621 gttttgttga cttggaaagt ttatcttctt caaaattttc cttgtatatt tttgcctgaa
    16681 tacgaggttg aatggtgtct cccatttcct ccgtttttcc tgaccacctt actcccattt
    16741 gttgcaaact atcaataata gccagatttt caggaaattc tgtcttttcc tgattgatta
    16801 aactttgaga taggcaaata cttgggtcaa aagtcacaaa aaccgctctc ttactgcgag
    16861 cataggactt aatagactga atggcaaaat tcaagagttc tttatcccca taatccaata
    16921 taggtcctct tgggatgtaa aacattttat agcctagcgg aagagttcta atcaaaatac
    16981 tagctgtcgc cagtaatttt tcttccctgt aaacaccaaa cttctcatgt tgccaattag
    17041 acttaacttc ctcccaagca ctactttgta atacattggc taattcatgt tctttgacaa
    17101 actgatcata ttctaatgtg ggaatgccaa tttgataacg gtacatttcc tactctcttt
    17161 cctccagtat tttattacta ttctactact ttcgtttgaa aagtctagtg aaaacatatt
    17221 ttattttagt tctactcccc ctatgctgaa gttcaaacta agtcagtctc tcttctaatt
    17281 tgaaatcaat tggtagggtt gttaaaaaac cttccaattg tttttcccaa taataccact
    17341 cgtgagttcc agcgctatgg ctataggtca catcaaaacc tagttttttg agatttttca
    17401 ctgcgagatt attggcttcg tacaagaaat cctgttcgcc acaccacgcc caaagtttgg
    17461 tctttttatc cgatttttta gccagacttt caagagaata gggactagtt gtccagtctt
    17521 taatctctcc aaaaacacct ctccagtatg ctggcgttcc cagattttga ctctcaggag
    17581 aaaaatcttg aaagctcaat gcacctgaaa agctagctgc atgagaaaaa cgatttgtcg
    17641 taagagccag tttgaagcag ccgtagcctc ccatagaaag accagcgata aaggtctttt
    17701 cacgcttgct agtcacatta gggaagaagc gtttcagaac ctgtggcaat tcctctgcta
    17761 gagccgtgta gtagtcaaaa ccatactggg tatcggtgta ccaaccattg ctggtattgg
    17821 gcataacaac gatgagatta gttcctcgaa gcaagcgttc tacattggtc cgcttaagcc
    17881 aactattatg atttccagac atcccgtgca aaaggtacaa gacgggaata tcttcacact
    17941 ctggttcttc cactcgattg gcatcagggt agaggacatt caccccccac tccatatcca
    18001 atacttgtga gtaatactcg attttcatta ctgccatgat tttctccttc tatttttcta
    18061 taagaaaaag ccgaaactcg actttctctc tgtttatttc aacgattatt ttgcttccat
    18121 gactactggt aaaatggctg ggcgacgctt ggtttgatca aagaggtact tggtcagatt
    18181 gtcacgaacc ttacctttga gatctgccca gtcaaagtca tctccttgaa gatactcttc
    18241 taccgtttgg ttaatcaatt ctgaactttc acggagaata tcgcgactct tcttgagata
    18301 aacaaatcca cgcgtgtgaa cacgagccct agccacaatt ttcttctcac gacggttgac
    18361 tgtaatagcc acgatgaaaa ttccatcctc tgacaagacc ttacggtcac gaagaacaac
    18421 atttccaaca tcaccaatgg cattcccatc aatcaagata tctcctgctg aaaccgatcc
    18481 agctggaaca aagtctccat tctcgtaagc catagtcgtc ccctttttag gaatgaagat
    18541 gcgttctggc aacatcccaa ctgccatggc agccttagcg tgagcatcca actcacgata
    18601 ctccccttgg acagggaaga ggtacttagg ttgcaaaaga ttgatcatca gctgcaaatc
    18661 acgcacattt ccgtgccctg atacatgtaa actttgggta atcaatttga caaccccacc
    18721 tgcctgataa atcatatttt ccacacgcgc aacaaaggct tctttagcaa tagacggagc
    18781 cgtagcaata tagaccaggt ccccatcctt gatttctaca taacgatggc gaccaatcga
    18841 catcttacga agtccattga taggctcacc catacgacct gtctcaagaa taatcaactc
    18901 atggtcttca aagcgagaca tatctttagg cttaatcaaa agaatttcgt tggctaaaga
    18961 caacttctta agacgaatcg ctgtgcggac gatattttca atatcaaatc ctgtcaagac
    19021 gatacgtcga cctgttttat ccgcagcgtc aaaaatctgc tgaatacgag aaagattact
    19081 ggaaacagct gcaacgatga tacgaccttc ccagtcagca atagtttggg taatttcatc
    19141 cctaacttca ctttcactag ccacctgaat attgctgtct gcattggccg aatcactgag
    19201 gagagccagg acgccgtcac gaccaatctc tgccaaacga gcaaaatcag ttgcataaga
    19261 ttcactagcc gtttggtcaa atttgaagtc acctgtataa acgatgcttc cttccgatgt
    19321 cttcaagaca attcccagac tctctggaac ggagtaagtc gtagggaaga aggaaaccac
    19381 tgtcccacca aaatcaatct ccgtattctc atcaatgaca tggaaatcat taaatttctt
    19441 aacggcatca tttcctttga caaagagctt tgccaactca atggtcaact cagacccaaa
    19501 tacaggaact ttagcctctg ccaagagata aggtagagca ccaatggcat ccgcatgccc
    19561 gtgggtcaag aaaaccccag caatacggtc gctattttca aaaaggtaat ccatgtttgg
    19621 aatcaccaca tcgaccccta attgttcatt ttcaggatat tttaacccta cattcaaaac
    19681 aaaaatggac tctccaattt cagcaatgta catatttttt ccattctcac gcacaccacc
    19741 aagtgttgtt aaactgatat tactcatttt tcctccaaaa tcttagttta tcttgattat
    19801 agggcaactc taccctctta ttctaaaagt tctaatactt tcagccgaat cttttcctac
    19861 cattatacca tttttttgat gattttcaaa atgaaggtct gttttcaaaa aagagttgat
    19921 ttcccaactc tttcctaact tctactcttt ttccatcaaa aaatcataaa tttcttgcac
    19981 cgtacccagc gccatcattt cttggtcttt aacggtttgt ttgagatttt ggtaaatatg
    20041 actttctcct atctcccgct ttggcgcctc tttattgact gtcatcacac catcttttat
    20101 cgtcacaaag cctagttctt caaatacttg aatcatcttg accagcaaga tttgttgaat
    20161 attaagatat gcagccaaat ctttcagctt gtagcgaata tcaaactctg ggaaccggta
    20221 aatagtcttg tacaatttgg caaactgatc tctagtccca taacctgtca gataataggc
    20281 cttgtcaata tcatttttga aatagacagc agagaaatac tgttcctgaa aaatggtctt
    20341 cagctgagta atatcctctg gaatgttttt tacgacaaca gcttcactag ccgcaagatt
    20401 tggcagttct ccaggaaaat ccaagactgg aacaccttct ggcaagactg cattttttcc
    20461 acgaatatta aaaagttgaa caccttccac tcgcgcatcc accatcatca actggagggc
    20521 agtttggcca ttccattggt tgacagacaa tttaaccgct aactctagat tcttggtttg
    20581 agaaaactct gtcgcccatc tgccttgacc aaaggctacc acttcaaaac tcgcctcacc
    20641 cttggaaatt ttcagcttta gatgggcatt acctgccccc atagtacgag cactttcgac
    20701 ctgaaaattc ttgatataaa aaataggttt ctgattatcc attccaaaag gagctaaacg
    20761 ttcaaaactt ttgaccgttt ccaagctaag tgcctccaaa tccaactctt catctaggtt
    20821 taacttattc ttgccaccag catctgcacc tttttcacga acataatctt ccaaaatctg
    20881 agataaatct gagagttgct caacttccag cgtcataccc gctgcacctg catgacctcc
    20941 aaaggcgatg aagaggtctc gatagggatc cagagcttca aaaatatcga ccgcttccac
    21001 actacgagca ctgcccttgg cacgaccgtc ttctatatta agaacaatga ctgtctgtcc
    21061 caattcttcc aataaacgac cagccacgat tcctagaacc ccaggattcc agccttcctt
    21121 ggccaagacc tgaaccttct tctcaggatc caccatggtc ttggcttctt catagataga
    21181 ctgaacgatt tccttgcgct cttcgttttt ctggtgaatc ataagggcaa tctcatgcgc
    21241 ttcctcatca tcaaatccag tcaacaaatc aatggcagga ttaggatcat ccaagcggcc
    21301 caaggcattc aaacgaggag caatctggaa accaaccatt tcttctgtta cttcgttggc
    21361 agcaatccca gccatgtcta gcatttcttg tagaccaata cgctgagtgt ggcccaacat
    21421 ttccagacca tattgcacca aaatgcgatt ttcatccgtc agactcacca tatcagcaat
    21481 tgtaccaata gcgaccaaat caagcaattc cacttgcact tcttctaaca gggcacaagc
    21541 caacttgaaa gcaactccac aaccagccaa atatttaaaa ggataatccg catctggatg
    21601 ttcaggatgg acaatagcat aagcatctgg cagggtttca ggcatggaat gatggtctgt
    21661 cacaatgaca tctactccca tagactgagc caatgcaata gcctcatgac cagcaacccc
    21721 attgtccacc gtcacaatca aggaaattcc ttcttgctcg ataaagtatt tataaacact
    21781 agcattaggg ccatagccat cggtaaaacg atttggcagg taaactcggc actcagcacc
    21841 aagttgttcc aaactttcct tcacaataga agccgaagtc atgccatccg catcatagtc
    21901 tccataaacg agaatatttt ccccttcttc aatagcctga cgaatccgct ccactgcctt
    21961 gtccatatca tggagcagat aagcatcatg taagtcctcc aaggaaggtt ctaaaaactt
    22021 cttcagactt tcttggtcct gaatccctct ctcaaacaat aaccgagcca cctcaggacc
    22081 cagtccagcc ttcttggcta tctttgtaaa atccgcatct tctacctgca gggcaaactg
    22141 ccattcataa gtaggtgtta tcaaaaagac atccactctt tctaagaatt ctattgcttc
    22201 attataacat gatttagacg ttttctctat ccagattgct tttttataac attttatagt
    22261 atattgaaac tagaatagta cacctctact tctaaaatat tgttagaaat cgatttgact
    22321 gtcccgatcg atttgtcctg ttcttatctc actatagtga ttttttcaga tatggtttga
    22381 caagaacatt cttttattgt agaattgtgt tataaaatct aacataaagg agatctctca
    22441 tggctttagt agaatttaaa aacgtcgaaa aatattacgg agactaccac gcactccgca
    22501 acatcaatct ccgttttgaa aaaggacaag ttgttgtcct gcttggacct tctggctctg
    22561 ggaagtccac tcttatccgt acgatcaatg gtttagaggc tgttgacaaa ggaagtctcc
    22621 tagtcaatgg gcaccaagtt gctggtgcca gccagaaaga tttggtacct cttcgcaagg
    22681 aagtcggcat ggtttttcaa cattttaacc tttatccaca caaaactgtg ttagaaaacg
    22741 tgacacttgc gcccattaaa gttctaggaa ttgataaaaa agaagctgaa aaaaccgccc
    22801 aaaaatatct ggaatttgta aatatgtggg acaagaaaga ttcctatccc gccatgctat
    22861 ctggtggaca aaaacagcgg atcgccatcg ctcgtggtct tgctatgcat acggaactcc
    22921 tcctctttga tgaaccaaca tctgctcttg atcctgagac tatcggagat gttctagcag
    22981 ttatgcagaa actggcgcat gatgggatga acatgatcat cgttacccac gaaatgggct
    23041 ttgctcgaga ggttgcggac cgcattatct ttatggccga cggagaagtt ttagtagata
    23101 cgacagatct ggttgtcggt gtcaaacaag acgttcccaa ttttggttac aaagatccca
    23161 agaccggtac ttattctggt atcgaaaccg acttggccaa gatggtagct gatgaactca
    23221 aggtcaagat tcgctatgtg ccggttacag cacaaacccg cggccccctt ctagacaatg
    23281 aacaggtcga tatggatatc gcgaccttta ccatcacgga cgaacgcaaa aaactctaca
    23341 actttaccag tccctactac acagacgctt ctggattttt ggtcaataaa tctgccaaaa
    23401 tcaaaaagat tgagaaccta aacggcaaaa ccatcggagt cgcccaaggt tctatcaccc
    23461 aacgcctgat tactgaactg ggtaaaaaga aaggtctgaa gtttaaattc gtcgaacttg
    23521 gttcctaccc agaattgatt acttcccttc acgctcatcg tatcgatgcc ttttccgttg
    23581 accgctctat tctatctggc tacactagta aacggacagc actactagat gatagtttca
    23641 agccatctga ctacggtatt gttaccaaga aatcaaatac agagctcaac gactatcttg
    23701 ataacttggt tactaaatgg agcaaggatg gtagtttgca gaaactttat gaccgttaca
    23761 agctcaaacc atctagtcat accgcagatt aaggaggata ccctatgaca gatttatcat
    23821 cttggacagc ctattttcag gattttggac aatttttcaa tggtttcctc tttactctcg
    23881 ccctagcgat tggatccttt atcctcgcca tggtcttagg aatcttcttt ggtgccatgt
    23941 caaccagtaa acgtccgatt ttgcgaatct tagctcgcat cttcgtggaa ttttaccaaa
    24001 acactcccct cttggtgcag tttgttattg ttttttatag cctacctctt atcagtgacc
    24061 atactatcat gattccgatt tattggacag ctgttctctg cgtgggactc tatcacggcg
    24121 cttataccgc tgaggttatt cgttcaggga ttcagtctat tcctagtggt cagatggagg
    24181 tcgccttgtc gcaaggtttt acctatatca gtgccatgcg cttgattatc ttgcctcagg
    24241 ccttccgcat cattctccct ccattgacca accaaattgt taacctcatc aagaacacct
    24301 ctactgtagc tatcatctct ggagtagact tgatgtttgt gactaagtct tggtcggctc
    24361 tcaacggaaa ctatatccca gcctttttag gtgttgctct tctctacttt gccctatgct
    24421 tccctgttgc ccagtttggt cgcaagatgg agcaagccaa caaaaaagcc tattcacttt
    24481 aggaggttac tatggaatct attttagaag ttttaagccc agataaccta gtctttatct
    24541 ttaaaggatt tggcttgacc ctctatattt ctctgattgc catcatcctc tctactatca
    24601 tcggtaccgt gcttgccgtc atgcgaaatg gaaaaaatcc tatcttacgc attatttcca
    24661 gtatttatat cgagtttgtg cgcaacgttc ccaaccttct ctggattttt actatctttt
    24721 tggtgttcaa aatgaaatcc acgccagcag gtattacagc ctttactctc tttacatcag
    24781 cagccttggc tgagattatt cgaggcggtc tcaatgccgt agacaaggga caatacgaag
    24841 cagggatgtc acaaggcttc acctcagccc aaatcctcta ctacatcatt ctcccacaag
    24901 ccatccgcaa aatgctgcca gccatcattt ctcagtttgt aaccgtgatt aaggacacca
    24961 gtcttctcta ctctgttatc gccctacaag aactctttgg agccagccaa attctcatgg
    25021 gtcgttattt cgaaccagag caggtcttca gtctttatat cctgattgcc ctcatctact
    25081 tcagctttaa cctagccatt tcgagcctgt ctcatatgct agcaaaacgt tggcaacaag
    25141 ctgcagaata atactcttcg aaaatctctt caaaccacgt cagcttcacc ttgccgtata
    25201 tatgttactg acttcgtcag ttctatccac aacctcaaaa cggtttttga gcagcctgcg
    25261 ggctagcttc ctagtttgct ctttgatttt cattgagtat aaacagccac tctccagttc
    25321 aattggagag ttttttataa gaacagagat ttacacacct agatttgaca ccatacattt
    25381 tctatgataa aatgtaacaa atttatttac aacaaatcga aatctaaatg aaatggaatt
    25441 ttctatgaaa tcaatcaaag gactactctt tatcatagct agttttatct tgactctttt
    25501 gacttggatg aacacttctc cccaattcat gattccagga ctagctttaa caagcctatc
    25561 tctgactttt atcctagcca ctcgtctccc actactagaa agctggtttc acggtttgga
    25621 gaaggtctac accgcccaca aattcacagc ctttctctcc atcatcctac taatctttca
    25681 taactttagt atgggcggtt tgtggggctc tcgcttagct gctcagtttg gcaaccttgc
    25741 catctatatc tttgtcagta tcatccttgt cgcctattta ggcaaataca tccaatacga
    25801 agcttggcga tggatccacc gcatggttta cctagcctat atttttggac tctttcatgt
    25861 ctacatgatg atgggcaatc gtctccttac atttaatctt ctaagttttc ttgttggtag
    25921 ctctgccctt ttaggcttac tagctggttt ttatatcatt tttctatatc aaaagatttc
    25981 cttcccctat ctagggaaaa ttacccatct caaacgctta aatcacgata ctagagaaat
    26041 tcaaatccat cttagcagac ctttcaacta tcaagcagga caatttgcct tcctaaagat
    26101 tttccaagaa ggctttgaaa gcgccccaca tcccttttct atctcaggcg gtcacggcca
    26161 aactctttac tttactgtta aaaattcagg tgaccatacc aagaatatct atgacaatct
    26221 tcaagtcggc agcaaagtaa ccctagacag agcttacgga cacatgatca tagaagaagg
    26281 acgagaaaat caggtttgga ttgctggagg tattgggatc acccccttca tctcttacat
    26341 ccgtgaacat cctattttag ataaacaggt tcacttctac tataacttcc gtggagatga
    26401 aaatgcagtc tacctagatt tactccgtaa ctatgctcag aaaaatccta attttgaact
    26461 ccatctaatc gacagtacga aagacggcta tcttaatttt gaacaaaaag aagtgcccga
    26521 acatgcaacc gtctatatgt gtggtcctat ttctatgatg aaggcacttg ccaaacagat
    26581 taagaaacaa aatccaaaaa cagagcttat ttacgaagga ttcaagttca aataagtact
    26641 catactctcc tgggaattct aggagagttt tttatagcca aatgaaataa gtccggaacg
    26701 atggaatttt ggcattcaaa tcaattgata agaatgtttt atagtggagt gaaataggat
    26761 acgaacaaat tgattaggaa aatcaaatga atttatagaa atcttttagc agttatgttg
    26821 tcctattctc gtttcaaaac gctatagaag cagcattgta ctagtctaga ttcagtttac
    26881 tatactaaaa cgagtagctt gaaatcaaaa aacccaccct cacaggcagg ttttatctgt
    26941 attattcagc tagattatgc tttaccttct gaaccgaata cgtcgatacg ttcttcaacc
    27001 gatgcttgga tagcttttac accgtcagcc aagaattttc gtgggtcgaa gagttttttc
    27061 ttgtcgtatt ctgcttcgtt tgcttcgtag tcacgagcaa atttacgagt tgcgttagcg
    27121 aatgcgattt ggcattctgt gttaacgtta actttggcaa caccaagttt gatagctgct
    27181 tggatttgct catcaggaat acctgatcca ccgtgcaata cgattgggaa tcctggaaga
    27241 gcttctgtca atttttgcaa gtggtcaagg tcaagacctt cccagtttac tgggtaaggg
    27301 ccgtggatgt taccgatacc agctgccaag aagtcgatac cagtttcaac cattgcttta
    27361 gcgtcttcga ttggagccaa ttcaccttta ccgatgattc cgtcttcttc accaccgata
    27421 gtaccaactt cagcttctac tgagatacct ttagcgtgtg ctttttcaac aacttcttta
    27481 gccaatttaa ggttttcttc aactggaagg tgtgaaccgt caaacatgat tgaagtataa
    27541 ccaacttcga tacactcaag tgcatcttcg tagtgaccgt ggtcaaggtg gatagctacc
    27601 ggtacagtga tacccattga ttcaacaagg ttagcgatca agttgcgagc aactttataa
    27661 ccacccatgt atttagcagc acccattgaa gtttggatca aaactggagc ttttttagct
    27721 tctgctgcgc gcaagatagc ttgagtccac tcaaggttgt ttgtgttaaa tccaccaact
    27781 gcataaccgt tgtcacgggc tgcttggaca aatttttctg ctgaaacgat tgccatttta
    27841 tcaggcctcc tgtatatttt tatgggtcat cccatttaca ttgttcattt tatcactttt
    27901 tgccaaaaaa atctagtttt tcccgcagtt tcgattgatt ttcttctaac tccatctatg
    27961 taaacccttt ctctccctag tcttgggcga cttttggaaa agctataaag aaggttaaac
    28021 gattctcctc catctcgaaa cgataagcta atttttcatg ttctaataga ctcttaacca
    28081 caaagagccc cataccagac cccttgacct tgcgactagc attgccagaa aaaaactggg
    28141 ctagtttttc ttgttcctct gagctacagc tattttcgat aaaaagttct ccttctcttt
    28201 ctccaattcg aactaagcca cctggaacag agtgcttaat ggcattgctg atgagattag
    28261 aaagaatcaa cttcataact gatgggttta gataagcctg ctgatgggtc aaactattgt
    28321 ctatctggag ctctctttcc ttggctagca aggcataatc tttgaccaga tttttcgtca
    28381 tctggaggag gtcaattgtt tccctatcat ctcgcaattc ctgcacagaa gagagggaaa
    28441 gtatctgcag aacatggtga ttgagttcat ccacaatccc caaggcaact cccagatact
    28501 ggtctctatc cttataacga ccgatattct ctctcatatt ttcgattagg attttcaaac
    28561 tagccagcgg tgttttcaat tcatgagaag ctcctcgtag gaattcgacc ttcatcttct
    28621 ccagctggag aatggcttca ttcttttcat gcaagtccgc aataacagtc aagagatgct
    28681 ggtagaggct attgatttgt tccttgagat tacctatctc atccttagaa tccacgcgca
    28741 atcgcacttg ggaatccagg tccatcatcc gacgggtcac ccgcttgatt tccaaaatcg
    28801 gtgcaacaat agtccgagcg tagatgtagg ccaccaagag ggaaatcaga aaggaggcca
    28861 gcaaggtata gggaagaaac tggagactga tttgctccgc ttccttttgt aaatccatgg
    28921 aagctagaaa ctggagaatc atagtaccac cgtcttgcgt tttcacctcg cgctcctcaa
    28981 taaagagaga ggttgtctgg cggtctgtgt ccagaggaag actgtccttg acttctaact
    29041 tgtcctcggt catctcacct ttgacggtcc ccttgatatc actagtctgg gaatacaagt
    29101 ctaacacttg ctcgatactc tgcctatctt tcccttctag ggactgggca atggctgttg
    29161 ccttttgacc aatggtttcc tgacgatgac tcagataagt cgaaggaaaa agaaaataaa
    29221 tagctaaatg aaggcagata accagaacac taaatatcga gaaagtatat ataaatatct
    29281 ttgtaaataa acctgttcgt ttcattttcg ctccaattta taaccaacat tgcgcacagt
    29341 gaggatacaa tccaagtcta gctttttccg caattccttg atataaacat caataacacg
    29401 gtcaaaggga acctcatctg tcgctttcca gacggcatcg ataatctgag accgagtcaa
    29461 ggcccggcct tcatttttca ctagatagtc cagaatttcc aactctttgg cattgatagg
    29521 cacttcttga cctgcgaggc ttgcactgta gctttcaaag tccaccttgg tatccttgta
    29581 agaaaagatt cgtcctgtat cgtagtagcg cttgaaaatc gcgtccaccc tcacttttaa
    29641 gagggagagg gagaaaggtt tttccagata gccatctgcc aaagaggcaa aggcactcat
    29701 cttgtattcc tcatcctgaa aagctgtcaa catcaagaca ggaacctgac tggttttacg
    29761 aatctcagct aggacttcta ggccgttaag cttgggcatc tggatatcca gtaaaaccag
    29821 ggccacctca tagctagaaa attgctccag agcttcctga ccgtccgctg cctcaatagt
    29881 ttcatagcca caatccgtca aataatcact gaccccctca cggatcatct cttcatcttc
    29941 tacaattaaa attttcatac tttaactgct ctctattttt tatttttctt agaataaata
    30001 cctactctat tttctattat agtctcttgc tggccttttg tctgtaagca actgaccact
    30061 agataaaacg ttgtgaaatt cctttctcat aaatttcata tatttagtat attatattta
    30121 agcactaaag tacaaagaaa acaactgaaa gcaatgattt tcaccactgc tttctgcttc
    30181 atttatcatt ttaaaacttt cagttgctag cctcattttt cactctggca tcggtattat
    30241 tcaccatcca gcaagagttc ttttggttgt tttctaagga gattacttga agcaagcgcc
    30301 ataacggcca ccactagaac caaggcaagg acaaagacga tgataaaatc tgatgtctga
    30361 atagaaatgt ctaggctcga taaggtctta ctaaagccat ctacttctgc accgccacca
    30421 aggttagagg cttgaaccgc cttactagcc tgtttggcaa cacctgaagt cacattggca
    30481 aggacagtgt ttccaattgc acgggcagtg taattagcta ggaagtaagc agaaactaga
    30541 gcagggatag caatcaagat agattcggtg atgaattgac ccaagatact tgcctgcttg
    30601 aggccgatag agaggagaat tcccacttcc ttgcgacggg cgttgatcca aaggctgagc
    30661 aagagggcaa ggaggagaac tgagaagctc aagctacccc agaagaggag gttggccatc
    30721 ttgtacatac cagagataga ttgctcaaga gctgggtagt tagaggagct cttgacaagt
    30781 gtgtagctct tccagttgat accactgatg ccattcaact ctttcataac atcatccaag
    30841 ttcttgtctg ctgttacaaa gaaggttgcg tccccataaa tggctgtgtc ttctgtgtat
    30901 ccataaagtt ttgcagcagt gtcaatgtct gtaatagctg tgttttcgta aagttcttgt
    30961 gagtaggtta ctgctgactt attatgacca tcaaagagtc ccttgattgt cacttcaact
    31021 gtttccttgg ctcctttttc attatctgca tcgtagatat tagagtccag tttaaccttg
    31081 tcccctactt tccagccgtg tttggctgcc aagtccttgt gcaagaggat tttatccttg
    31141 tcgtcgttgg ttaagtgctc tccttcgact agtttataag aaccagagac aaacttgtct
    31201 tctttaaaag agtcattgac acctgtaatc atcaagctac ttccaaaacg tttggcacga
    31261 tcagcagtga gattcttctt ggtttctggc gtttcaatca ggtcatatcc agtcaaatct
    31321 ccgatagcgt tgatacgttt gacataagac tcgatggcct tgttttcggt gatttttttg
    31381 atgtcttcac ccttgatatt cccagcacca cgaggcgttc cttggttgac gcgacgattg
    31441 atttgcatgg agaagctatt ggtgatattt ttaaaggtct cctgagaagc cttggcagta
    31501 gctcccttga ttgacaagcc gaccaaactc aagctcgcca tgaggagaat aatcaggaag
    31561 atgacaatcg atttaaaaaa cttccttgta acataggcaa atgcgttgtg taacatagat
    31621 tccctttcta gattttgttt taatcattct atcaaaataa gctcaaatta tttactagta
    31681 ttgcgcgttt cagtcagttt cttatccttt aattcaagtg taatatctga cgcttgtgcc
    31741 acttctttac tgtgagttac gacaatcaca catttacctg ttttctgggc aagtgatttg
    31801 agcagttcga caatatctcc agcagtttta ggatccagat ttcctgttgg ctcatcagcg
    31861 agaataactg gagcttctga tactaaacta cgagcaatgg caacacgttg ttgttgacca
    31921 cctgacaact ggagaacatt ccgcttgatc tgactttcat ccaaaccaag ctcaagaagt
    31981 gtatccttgc tggccttttt gttaaccaag cggatatttt ccagtggaga aagataatct
    32041 atcaagttat aattttgaaa gaccaaggaa atatggtgca tgcgatggta agaatagccc
    32101 ttcttacgaa tatcctctcc ttgaaaaagg atagaacctt caacaggact atctagacca
    32161 gcaagtaggg acaagagagt ggattttcct gctcctgact ccccaataat actgtaaaat
    32221 ttcccgggtt caaaattata attgatctga tataggactg cttcagcagt gttcttataa
    32281 cggtaggtaa ggtcttgtaa ttgtaataaa gtcatgattt ctccttctta tactcaatga
    32341 aaatcaaagt tcaaactagg aagctagctg caggttgctc aaagcacagc tttgaggttg
    32401 cagatacagc taacgcggtt tgaagagatt ttcgaagagt attaactaat agatgataaa
    32461 atttctttag gcgattttct aaataagaat aggaaacaaa gtgctacaga caagcaacta
    32521 agcagaacta gaaagacata ggattctgca aatgatagga tattagttga tagactgctt
    32581 gctttagcta gcgtgtcttg taagcttgcc tgctctccac ttgctagtag agtttggagt
    32641 aggtaagatg tgattgcgtt tcctacaaca aatgctggaa gcaaagcgcc gagagatacc
    32701 aaaactacct ctaaacagaa ttgtaggaag attgaggtct tgccttttcc aagtgccagt
    32761 aaaatcccca cttcgtagac cctttctctc aaccagagag acaaaacaag aattaaggcc
    32821 cctgctcctg ctatcaacat cccataaagg aagatggtca gaaaggtttg gaaggttgca
    32881 actgagtctt tgatttgttc aaaagccttg ttttcctttt cgacttggta gccttgattt
    32941 tccaaggcca agttttctac ctgcttcacg agtccgtcca tttccttagg attttctaca
    33001 tagaagcgcg ctgcactgac ttgcggttca ctatttccca aaagagtttg gctactgtca
    33061 tagtctgtaa agacttgatt ttcactgaag tcagaagaca agcctgtgaa tttctcctgt
    33121 tttttaccag aaaagatgcc gacaatttca aactcaactg tttgtccttt accagcttcg
    33181 gactgaccag catctaaggc aatcttgcca tgaagcgaaa gaccattctt cttagccaac
    33241 tcttcgtgga tgagaatttt ctttgaatcc cctttttgaa gatgtctccc ttcttttaga
    33301 ttgaaagccg aactggtaaa ggtcacatcc ttggatgaat cctccagagc tgttaagcta
    33361 accaagttct tgtccgcagc tgacaaatca tcacgttcaa cgctctgctc gccactcact
    33421 gcttccttgt cttttagttt tgcgaccgtc tccagttcag gagaaacatt ttccagtccc
    33481 ttaatcttgc ttacagatgc taggtctgac aacttgaatg tctgaccatt ttctatcttc
    33541 ttaattgaaa aagaggtatt gagtgatttg taaagattgg tttccactgt tttgttggac
    33601 ttcatcagag tcaaacaggc tgagattccg gccaatagga ccaataaaat cagaaataaa
    33661 ataaaacttc tcagtcgttt tctgctgaca taagcccaag atctttggat tggattcatt
    33721 tgtcacctcc atatttgtaa gactattata aaacccaaat atgaaatgtt tatgaaatac
    33781 gaaaaaaaaa atatcaaaaa gagcaacggc tttcaccgtt gctctttcaa tcactattct
    33841 agctagtttc cgactagttt aagcttgact tatcttaact gagccagaat agctttgagt
    33901 ttctcaatca actcatcttc tgtgtgatca gactctttct cattggctag gaaaggaact
    33961 gcttgagcga gagcttctcg tgcttggtcg aggagtttcg aatctacact tgtaaggtcg
    34021 ctttcttgca gaaccttagt caatcccttg gtcaatttct tgagttctga ctctttctta
    34081 cgacgcgcga tgaggaagag aaggagacca agaaaggcaa gcaggctagc aatcatcgta
    34141 ccatatggaa gatgaccttg ctctttgctt gtttcttgca tttcctcttc atccttagtc
    34201 gcttgcttgc ctttcaagga ttcgagtgct tctttgacct tagcagtaat ctctctcaag
    34261 cctgcctctg tcaagtcagt atttctaagg gctacttgac tctctacgag aatgatctta
    34321 gcagcatcga tgacagattg atcaatacca tccaagtttt tcaagcgagt ttccaattct
    34381 tcaaccaatc gagcaagttc aaacttgtca acaggacgag cctcactagg agttgctgta
    34441 ttgcttgaat tatttggagt gcttggagaa actggtgttt cttgagctgg agtacttgct
    34501 tgagcctgtc ctccaacagt agacgatgga gtagcagagg tcgtgttcct actatcgtcg
    34561 gtcgtcacat taggcgctgg agtgttggca ctgttatctg gtgtcacact gttactattg
    34621 ctatcagcag gggtggtagt accaccgttg ttggtgttag cactattgtt agtgttgcca
    34681 ctgttgccat ccttacttgg tacagaagtt ggcttatcta c
//
LOCUS       DAVKDZ010000014        34640 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000014 DAVKDZ010000000
VERSION     DAVKDZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34640
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.014"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(713..1285)
                     /locus_tag="VWZ30_000660"
     CDS             complement(713..1285)
                     /locus_tag="VWZ30_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334446.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIHSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGNWFVNDVALE
                     SAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(1908..2756)
                     /locus_tag="VWZ30_000661"
     CDS             complement(1908..2756)
                     /locus_tag="VWZ30_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9334447.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYNKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            2908..3186
                     /locus_tag="VWZ30_000662"
     CDS             2908..3186
                     /locus_tag="VWZ30_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334448.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            3188..5251
                     /locus_tag="VWZ30_000663"
     CDS             3188..5251
                     /locus_tag="VWZ30_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9334449.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRICVTD
                     NQMNSRLLQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLTRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(5329..6585)
                     /locus_tag="VWZ30_000664"
     CDS             complement(5329..6585)
                     /locus_tag="VWZ30_000664"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU9334450.1"
                     /translation="MHIFDELKDRGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQELDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            6727..9189
                     /locus_tag="VWZ30_000665"
     CDS             6727..9189
                     /locus_tag="VWZ30_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9334451.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYYQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSTWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSRARR"
     gene            9254..10165
                     /locus_tag="VWZ30_000666"
     CDS             9254..10165
                     /locus_tag="VWZ30_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9334452.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVSVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            10395..11093
                     /gene="dapD"
                     /locus_tag="VWZ30_000667"
     CDS             10395..11093
                     /gene="dapD"
                     /locus_tag="VWZ30_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEU9334453.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            11161..12291
                     /locus_tag="VWZ30_000668"
     CDS             11161..12291
                     /locus_tag="VWZ30_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEU9334454.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVDAVSSFLKKKAAE"
     gene            12303..12842
                     /locus_tag="VWZ30_000669"
     CDS             12303..12842
                     /locus_tag="VWZ30_000669"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU9334455.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            12826..13503
                     /locus_tag="VWZ30_000670"
     CDS             12826..13503
                     /locus_tag="VWZ30_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU9334456.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIILRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(13570..14469)
                     /gene="galU"
                     /locus_tag="VWZ30_000671"
     CDS             complement(13570..14469)
                     /gene="galU"
                     /locus_tag="VWZ30_000671"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU9334457.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYKRTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFTGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(14491..15507)
                     /locus_tag="VWZ30_000672"
     CDS             complement(14491..15507)
                     /locus_tag="VWZ30_000672"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9334458.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            15719..16158
                     /locus_tag="VWZ30_000673"
                     /pseudo
     CDS             15719..16158
                     /locus_tag="VWZ30_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206583.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            complement(16273..16698)
                     /locus_tag="VWZ30_000674"
     CDS             complement(16273..16698)
                     /locus_tag="VWZ30_000674"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9334459.1"
                     /translation="MNNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(16766..17416)
                     /gene="phoU"
                     /locus_tag="VWZ30_000675"
     CDS             complement(16766..17416)
                     /gene="phoU"
                     /locus_tag="VWZ30_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9334460.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELACARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(17431..18183)
                     /gene="pstB"
                     /locus_tag="VWZ30_000676"
     CDS             complement(17431..18183)
                     /gene="pstB"
                     /locus_tag="VWZ30_000676"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9334461.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(18185..19000)
                     /gene="pstA"
                     /locus_tag="VWZ30_000677"
     CDS             complement(18185..19000)
                     /gene="pstA"
                     /locus_tag="VWZ30_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9334462.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLVIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(18993..19856)
                     /gene="pstC"
                     /locus_tag="VWZ30_000678"
     CDS             complement(18993..19856)
                     /gene="pstC"
                     /locus_tag="VWZ30_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9334463.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(19974..20849)
                     /locus_tag="VWZ30_000679"
     CDS             complement(19974..20849)
                     /locus_tag="VWZ30_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU9334464.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGKYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(21016..22347)
                     /locus_tag="VWZ30_000680"
     CDS             complement(21016..22347)
                     /locus_tag="VWZ30_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEU9334465.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSALKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(22348..23055)
                     /locus_tag="VWZ30_000681"
     CDS             complement(22348..23055)
                     /locus_tag="VWZ30_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9334466.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(23139..23441)
                     /locus_tag="VWZ30_000682"
     CDS             complement(23139..23441)
                     /locus_tag="VWZ30_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9334467.1"
                     /translation="MTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            complement(23428..23649)
                     /locus_tag="VWZ30_000683"
     CDS             complement(23428..23649)
                     /locus_tag="VWZ30_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9334468.1"
                     /translation="MNKSYFNPLAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINL
                     AGKGRALYTSIYFLLRRNESFTFSPIDSR"
     gene            23611..24169
                     /locus_tag="VWZ30_000684"
                     /pseudo
     CDS             23611..24169
                     /locus_tag="VWZ30_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(24559..26250)
                     /locus_tag="VWZ30_000685"
     CDS             complement(24559..26250)
                     /locus_tag="VWZ30_000685"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU9334469.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILNLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIATELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKAQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            26386..26832
                     /gene="argR"
                     /locus_tag="VWZ30_000686"
     CDS             26386..26832
                     /gene="argR"
                     /locus_tag="VWZ30_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9334470.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLANETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            26883..29417
                     /gene="mutS"
                     /locus_tag="VWZ30_000687"
     CDS             26883..29417
                     /gene="mutS"
                     /locus_tag="VWZ30_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU9334471.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVAIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSCQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIREILEGMEQPALAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREESA
                     NLEYEIFMRIREEVGKYIQRLQSLAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IQKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLITGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAETAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIESGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            29564..31258
                     /gene="patA"
                     /locus_tag="VWZ30_000688"
     CDS             29564..31258
                     /gene="patA"
                     /locus_tag="VWZ30_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEU9334472.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLDALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNFYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQTIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQDGAVKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(31587..31850)
                     /locus_tag="VWZ30_000689"
                     /pseudo
     CDS             complement(31587..31850)
                     /locus_tag="VWZ30_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            32047..33813
                     /gene="patB"
                     /locus_tag="VWZ30_000690"
     CDS             32047..33813
                     /gene="patB"
                     /locus_tag="VWZ30_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEU9334473.1"
                     /translation="MKTVQFFWNYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNVVLYIGLILV
                     MFSRNVTLALITIASTPLAFFMLIFIVKMARKSTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLVNTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            33952..>34640
                     /locus_tag="VWZ30_000691"
     CDS             33952..>34640
                     /locus_tag="VWZ30_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9334474.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPLGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
BASE COUNT         9989 a         6575 c         7554 g        10522 t
ORIGIN      
        1 taattgtagg aaagaaggtg tttttatgat atacacactt aaattggtgt tgtttattac
       61 ctttcttgta ataagcttgt tacctgataa gatttttgga aaaaataaaa aaatttggaa
      121 aatagttttt gcaatattga cggcagtggc agcattgtca tttatgtact aagttatttt
      181 aagaatgtag ggaaataaac cctacattct ttttagtttt ttctgttttc taaattctat
      241 ttatccaagc gcttcaacat ttcttgcttc ttcgcttcaa gttctgcacg cttttcttcg
      301 atttcggcat gttttttctc gagttcagaa caacttgcac cattgctaaa ttcttttcgc
      361 catcaggaga tagggtgagt cgacatgtct attactcacc caaagcagtc ctacaaagca
      421 ggaattttct gttacttttt tggaaatagt aacgtttata cagctttgac acttcgtatc
      481 aaagcgccaa acacactccg agggatttac agaaagcaga aaaggaatga tctggtataa
      541 gatcattcct tttctctctt tttctttaag taattatata caatgtacga cgaagtcgtc
      601 attgcaatgc tgatccacca cctaaaggga actttaaaca acattgataa gataaagaat
      661 ataaacaacg aaaatacgtt atacccaatt aattttattg tatatctcat gattaaaagt
      721 taatccttcc gttgttagga atggcatcat ttttatccca taattgtgct aaataagtcc
      781 ccggtgataa taaattcata gcggattcta aagcaacatc atttacaaac caattaccta
      841 gatatctaga aattgctgaa cgaatagcac tttttgctgc atgttttcct tttactttaa
      901 ttagatttgc aaggcctgca gtagttcctc ctaatgctaa agctattgca gtatctaata
      961 gagcacccat ttgattaact gtaatacctt gccaagctgc tctaaatgga gagtatgtag
     1021 gtgggattgt ataatcgcct tgtaattgtc ggttaattac ttctttgatc cattgttgtg
     1081 agacgtctgg atgaaaagag tggatttcgt ttgcaagtgt attgatttgt tcttctgtta
     1141 gagaagtgac aggttgaagt tccatatttg tttcaatttg tgatacttgt tcagaagcgt
     1201 atacagctga aacacttgga atcgctgata caattaacac aattgacgtc aaaaaaaccg
     1261 aaataaattt cattaatttg ttcatgagct tttctccttt ttatttgtat ctgcttacat
     1321 tttattatat actgttatta tagtcaagaa aaatatgcta ttatgttaaa aaaatatttt
     1381 taaaaatata aatggacgga tttattttgg attttatttg ttattttgac ctgcctctat
     1441 attggtaacc atgatttgtt tactttcaat catcaagaat tctcttttcg tggtagcgtt
     1501 tggggtctgg tactggcctt atatcactta ttgttcattg ataagtttgt aatttcgaat
     1561 cgaaaataaa gattagaact atgattgact ggctactttt aatattgatt ttggaggaag
     1621 attttgtctc tattatttat tattttaaat ttatttattt tgtataagat ctattctttg
     1681 aggcgggaaa aatcgaaata cttgatttat acggcctata tcatatttgg ggtaaatgta
     1741 gtatatggta ttcaatggtt attaagagaa ctgatttcaa ctatttcccc ttaatgtgta
     1801 tggaaactat aacaaaacgc ataagattaa ggtttgtgta cctgttctta tgcgttttta
     1861 taatattgaa gattttcggt aagttttata cctttgggaa gtctagttta gaatgctttc
     1921 ccaaccaaaa tctctgcttc gatggtaatc tctgtcagtt ggctccagtc aatcttgctc
     1981 tggtcaacgt gaaagaggag aggagtcata ctgagtaggg cttggagttg ttctgctgtg
     2041 atagtcttag tcagagaggc agtttgacta gataggatgg taaagtgttc ctggaaatgc
     2101 tccttgatat cttggttaga atactccttg tttgtcagct ggtcctgtac tctttgacgg
     2161 atttctttga ggtgattttc agttgggata acctttatca agataccgtc tttggataaa
     2221 acgcgacgaa attctccata gttggcaggt gagaagatat caagcagaat atccatgtta
     2281 gcgtctttta taggaagtcg tgccaagtcg ccaacaaacc aattgactgc ccagttgggt
     2341 tcacttttag cagcgatttg gactgaatct ttggagatgt caaaggcata gaaagttttt
     2401 tcagagtgac tttcttgtag tttgcgagaa tagaatcctt caccacaacc gatatccaaa
     2461 attgttgtgg tagtttttga gcttgcaagc aagtcagata cagcatctaa gatagcttgg
     2521 taaaagccgg cttctaggat ttgttgacgg ttttgaaaat tttccttgtt atagttagca
     2581 gattgcttga tttgaggtgc tagattgaca tagccaaatt tcgccaagtc aaaagaatga
     2641 cggttgcagc acttgaaatt agtctctaac agagtcagat tttcttgaca gataggacag
     2701 gcaaaggcag tcgcagaagc aaaacgctga agtttgggct tgagatttgt attcatagtt
     2761 taagtgtatc aaaagaggca aatgaaagca actttaggaa taagtagatg ggagattcag
     2821 tagaaataaa actaattctc caatttatag aatgaaattg cttttatatc taaattgcta
     2881 tataataatg ataaggagga aataagtatg ttaaaagaag tattaaccgt tgcaaaagtt
     2941 gcgaaaaaat cttcattatt tttgggtggt gtcgcatttg gtacccttgg tttgaaaatc
     3001 ttagcaagta aggaagctaa aaaaggttat tctaaagctt tggctaaggc ttacaagttg
     3061 aaagacgagc tagatgcatc tgtttctgtt gtgaagcaac atggagacga tgtcttgcaa
     3121 gatgccaaat atttgtacga gcaagagaaa aaagaagagc aattagatag ccttataggt
     3181 gaataatatg tcttttaaag tgctacatag aggataccaa catatccgac tatcatcttc
     3241 tttttcactt accttggata ttcaagacta tcttcgttcc ttggcgagag atgaaaaggg
     3301 gattgagtct atccagtttt acatggatca acagcacttt actctacgca taaaagaagg
     3361 cttttctgta ttagataatg cagaagcctt tttaaaaaga attgataaag ggaaagtttc
     3421 tgagttgatg actcttccca ttcgtagaga agagagtgct tattctattg tttcaggtgc
     3481 agcggttaag cgtgtacttt ttcgtagttt tgtgccgtat cctattcgct atatatggac
     3541 ttgttatcag gctttgggtt atattagaga agcctatcaa acactagcgc gtaaggaact
     3601 aacgatggaa gtcttggact gttcggctat tttattgtct ttgtttatga accaatccaa
     3661 gacagctagc aatatcatgt ttatgcttga tttggggaat catttagatc agtggtcctt
     3721 gaaaaaaact gcaacagatt tagaacagag tcttcttgca aaagagagcg atgtattcct
     3781 agtacagggc gatacggttg ttagtatcaa gagttccgat gttcaaatag gagatgtctt
     3841 gatcttatct caaggaaatg aaattctgtt tgatggacaa gtagtttcag gtttaggtat
     3901 ggtcaacgaa agttccttga caggagagag ttttccagtt gaaaaaagag agtctgattt
     3961 ggtttgtgca aatacagtat tagaaactgg agagttacgc atttgtgtaa cagataatca
     4021 gatgaacagc cgtcttttac agctgattga gttgatgaag aaatctgaag aaaacaagaa
     4081 aacgaaacaa cgctatttca tcaagatggc ggataaagtc gtcaaatata atttcttggg
     4141 ggctgggctg acttacctat tgacaggttc tttttctaag gctatttctt tcctattggt
     4201 cgatttctcc tgcgctttga aaatctctac tcctgtagct tatttgacag ttatcaagga
     4261 agggttgaac cgtgaaatgg tgattaagga tggagatgtt ctggagaaat atctggaagt
     4321 tgatactttc ttgtttgata agacaggaac aatcacaact agttatccta tagttgaaaa
     4381 ggtgttacct tttggagact atagtgagga agatattctc agaatcagtg cctgtcttga
     4441 ggaacacatt tatcatccta ttgctaatgc catcgtcaag caagctgaga tagagggaat
     4501 tgaacatgag gaaatgcatg ggaaactcca atatatcgca agcaagggga tcaaatctca
     4561 tatagatggc caaccagttc ttattgggaa ttatgtcttg atgcaggatg agcagattca
     4621 tatcagttca gaacaaaatg ctttaattga agagtacaag agtcactaca atctcttatt
     4681 cttggcttat caaaatgaat tgattggaat gttctgcatt catactcctt tgagaaaaga
     4741 agcaaaaaca gccttggata aacttaaggc acaggggaaa aaattgattc tggcaacagg
     4801 agacaccttg actaggacag aggaattagt caaagatttg ccctttgatc aggtctatac
     4861 agacttgaaa cctgatggga aatttgagtt agtagagaaa ctgcagaaag caggtcacac
     4921 tattttgatg gttggagatg gattgaatga ctcagcggct ttaactctat cagatatcgg
     4981 tgtggtgatg aatgagagtg cagatatttc taagcagatg agtgatatct tattgttaga
     5041 taatcgtttg gatttcttcc aagagttgga ttcgttatca tcatctttgc aaacactcat
     5101 caagaaaaat attcaagata ccgttgtcgt aaatagtagt ttgattggct tcggcttatt
     5161 taattggctc agtccttcaa atctctctat cttacataat ctaacaacct tgcgcattgt
     5221 cctgcgtagc ctgtctatta aaaatagata gatgagagtt attaacagca aaaaggagtt
     5281 acctttctcg aggctaactc ctttgtttat agataaatgt tgaatagttt agtaagtcaa
     5341 tacaaagtat tttttcttcc cacgacggat aacagtcagt tcattctcta acttatcagc
     5401 gtcactcaag acatagtcaa gctcttggat gcggtcgccg tttacgtaga tggctccgtt
     5461 ttggacgtct tcacgggctt ggcgttttga gttaactata ccagatgaga cgagcagttc
     5521 cacgatattg ttgttttcgt ctgcctgtac ttggtagttt ggcacaccac gaagtccttg
     5581 tttgagctct ttgacagaaa ggtttttgat gtttcctgca aagagttgct cagtgatgtt
     5641 aagtgcttct ttgtaggctt cttctccgtg aacaagtgta acaacttcac gagccaagac
     5701 tttttgagcc aagcgttcgt gtggcgctgc ttcaaattgt ttacgaatat cttcaatctc
     5761 atcaagtgac aagaaagtaa agattttcaa gaagcgaaca gcgtcagcgt ccatcacgtt
     5821 catccagaat tggtacattt cgtatggaga agtcttttcg ggattgagcc agacggcatt
     5881 tccttctgat ttaccaaatt tcttaccagt tgcatctgtg attagtggaa cagtgataac
     5941 gtgaccagtc ttgtccgctt tacgacgaag caattcggta ccagctgtca tatttcccca
     6001 ctggtcagaa ccaccgattt gaagagtgac attatggtct tggttaagga cgaagaagtc
     6061 gtacccttgc atgatttggt aagcgaactc agtgtaagaa attcctgttt cgattcgttt
     6121 ttttacagat tccttactca tcatgtagtt gaccgtgaag tattttccaa tatcacggag
     6181 gaagtcaatg aagctgatgc tgccaaacca gtcgtagttg ttgaccatga cagccttgtt
     6241 ttcgccattt tcaaagtcaa gaaaacgaga aagttgtcct tggatagact tgacccagcc
     6301 atctactgtg tcttttgttt ggagactacg ttcagcatct ttgaaggacg gatctccgat
     6361 gagacctgta gcaccgccaa cgagcgcata aggtttgtga cctgctagtt gcaagcgacg
     6421 acttgtcaag attgcgacaa ggtggcctag gtgaaggctg tcagcagttg gatcgtagcc
     6481 agtataataa gaaacttgac cttcttctag ggctttacgc aaagcttctt catcagtcgt
     6541 ttgaaatatc aaaccacgat cttttagctc atcaaaaatg tgcatgtgtc ttttctcctt
     6601 tttaaaatta ttgtttctac ctattgtatc acaaacttgg gcaatagcct agcagaatag
     6661 ggaagaaagt ggctatttta ttgaaaattt cccttttgtg gtataataga tagagtgagg
     6721 aaatccatgc aaaatcaatt aaatgaatta aaacgaaaaa tgctggaatt tttccagcaa
     6781 aaacaaaaaa ataaaaaatc agctagacct ggcaagaaag gttcaagtac caaaaaatct
     6841 aaaaccttag ataagtcagc cattttccca gctattttac tgagtataaa agccttattt
     6901 aacttactct ttgtactcgg ttttctagga ggaatgttgg gagctgggat tgctttgggg
     6961 tacggagtgg ccttatttga caaggttcgg gtgcctcaga cagaagaatt ggtgaatcag
     7021 gtcaaggaca tctcttctat ttcagagatt acctattcgg acgggacggt gattgcttcc
     7081 atagagagtg atttgttgcg cacttctatc tcatctgagc aaatttcgga aaatctgaag
     7141 aaggctatca ttgcgacaga agatgaacac tttaaagaac ataagggtgt agtacccaag
     7201 gcggtgattc gtgcgacctt ggggaaattt gtaggtttgg gttcctctag tgggggttca
     7261 accttgaccc agcaactaat taaacagcag gtggttgggg atgcgccgac cttggctcgt
     7321 aaggcggcag agattgtgga tgctcttgcc ttggaacgcg ccatgaataa agatgagatt
     7381 ttaacgacct atctcaatgt ggctcccttt ggccgaaata ataagggaca gaatattgca
     7441 ggggctcggc aagcagctga gggaattttc ggtgtagatg ccagtcagtt gactgttcct
     7501 caagcagcat ttttagcagg acttccacag agtcccatta cttactctcc ttatgaaaat
     7561 actggggagt tgaagagtga tgaagaccta gaaattggct taagacgggc taaggcagtt
     7621 ctttacagta tgtatcgtac aggtgcatta agcaaagacg agtattctca gtacaaggat
     7681 tatgacctta aacaggactt tttaccatcg ggcacggtta caggaatttc acgagactat
     7741 ttatacttta caactttggc agaagctcaa gaacgtatgt atgactatct agctcagaga
     7801 gacaatgtct ccgctaagga gttgaaaaat gaggcaactc agaagtttta tcgagatttg
     7861 gcagccaagg aaattgaaaa tggtggttat aagattacta ctaccataga tcagaaaatt
     7921 cattctgcca tgcaaagtgc ggttgctgat tatggctatc ttttagacga tggaacaggt
     7981 cgtgtagaag tagggaatgt cttgatggac aaccaaacag gtgctattct aggctttgta
     8041 ggtggtcgta attatcaaga aaatcaaaat aatcatgcct ttgataccaa acgttcgcca
     8101 gcttctacta ccaagccctt gctggcctac ggtattgcta ttgaccaggg cttgatggga
     8161 agtgaaacga ttctatctaa ctatccaaca aactttgcta atggcaatcc gattatgtat
     8221 gctaatagca agggaacagg aatgatgacc ttgggagaag ctctgaacta ttcatggaat
     8281 atccctgctt actggaccta tcgtatgctc cgtgaaaagg gtgttgatgt caagggttat
     8341 atggaaaaga tgggttacga gattcctgag tacggtattg agagcttgcc aatgggtggt
     8401 ggtattgaag tcacagttgc ccagcatacc aatggctatc agaccttagc taataatgga
     8461 gtttattatc agaagcatgt gatttcaaag attgaagcag cagatggtag agtggtgtat
     8521 gagtatcagg ataaaccggt tcaagtctat tcaaaagcta ctgcgacgat tatgcaggga
     8581 ttgctacgag aagttctatc ctctcgtgtg acaacaacct tcaagtctaa cctgacttct
     8641 ttaaatccta ctctggctaa tgcagattgg attgggaaga ctggtacaac caaccaagac
     8701 gaaaatatgt ggctcatgct ttcgacacct agattaaccc taggtggctg gattgggcat
     8761 gatgataatc attcattgtc acgtagagca ggttattcta ataactctaa ttacatggct
     8821 catctagtaa atgcgattca gcaagcttcc ccaagcactt gggggaacga gcgctttgct
     8881 ttagatccta gtgtagtgaa atcggaagtc ttgaaatcaa caggtcaaaa accagggaag
     8941 gtttctgttg aaggaaaaga agtagaggtc acaggttcga ctgttaccag ctattgggct
     9001 aataagtcag gagcgccagc gacaagttat cgctttgcta ttggtggaag tgatgcggat
     9061 tatcagaatg cttggtctag tattgtgggg agtctaccaa ctccatccag ctccagcagt
     9121 tcaagtagta gttctagcga tagcagtaac tcaagtacta cacgaccttc ttcaagggcg
     9181 agacgataat tctctaaatg aagtggccaa tcaatggatt gccacttttt tcctttcgtg
     9241 ttacaataag ggtatgaatc agtatcagaa aaagattgtt aacggaaaaa tttattcgct
     9301 cctatccggc ttaatatggg gaatctgtgg aattttagga gagtacttct ttactcatta
     9361 tcaggtgtct tcgggctgga ttacctctat gcgtttgaca ctggcaggga gtcttgtact
     9421 catttggtct gcaatacaat taaaatcgca agtgctagat atttggcgag acaagaaaaa
     9481 ttacctgccc tttttagcct atgctatttt ggggattttt tcagttcagt attttttcta
     9541 tctctgtgta gaatactcaa atgctacgac agcaactatt ttacagttta ttagccctgt
     9601 ctttatcctc ttttataatc gcttggttta tcaaaaacga gcgtcaaaaa gcgctgtttt
     9661 ctatgttttg gttgccatgc tgggtgtttg cttgatggcg acaaagggag atctctctca
     9721 gttatccatg acgccgctag ctcttataac aggtttgctg agtgccatgg gtgttatgtt
     9781 taatgttatc ttgccccaac cttttgctaa gcgttatggt tttgttccta cggttgggtg
     9841 ggggatgatt ttggcaggtt tgtttagcaa tgtcctctcg ccggtttatc agctttcctt
     9901 tactcttgat atttggagta tcttgatttg cctcattatc gctttctttg gaacggcttt
     9961 tgcttttttc atttccatga aggctgtgtc cttggtttct cctttggtgg tttccgttat
    10021 cagtgccagt gaacctctct cttctgctct cttgagtgtt ttgttcttag gattagtagt
    10081 ggattggtcc ctccttctag ctatagcctt gattatttta cccatgatat ttttgtctat
    10141 agaagaagcg aaagaaagta gataaaaagt cttttcttaa ttctaaaagt gtgctatact
    10201 agaatagtca ataaaacacg gggagatagt tgtgaagagt gtttttaggt tgtatcggta
    10261 ggggcttgct tttaccgaca gcacgtttta tctcacaacc ctaaaaaatc atacctaaaa
    10321 acagacaaaa aggtttcaaa tttgaggcct tttttggtag aataggtatc attataacga
    10381 accaggaggc acctatgact gctacaaaaa tgaacgctca agaaattatc caatttatcg
    10441 ccaatgctga aaagaaaacc agtgtcaaag taacctttga ggggcaactc gcaactgctg
    10501 tgcctagctc tgttgtcaaa ctaggaaatg tcctattcgg agactggaag gacgtggctc
    10561 cgcttcttga aggtttggta gaaaatcaag attatgttgt cgagcaagat gctcgtaatt
    10621 ctgcagttcc tttgctagat aagcgtgcta tcaacgctcg tatcgagcca ggtgctatta
    10681 tccgtgacca ggtggaaatt ggtgacaatg ctgttatcat gatgggagct gttatcaata
    10741 tcggtgctga aatcggtgct ggaaccatga ttgacatggg tgccatcctt ggtggccgtg
    10801 ccatcgttgg aaaaaatagc cacgttggtg caggtgcagt tttggcaggt gtgattgagc
    10861 cagctagtgc tgaaccagtc cgtgtcggag acaatgttct tatcggtgct aatgcagtgg
    10921 ttatcgaagg agtccaaatc ggtagtggtt cagttgtcgc agcaggagct attgttaccc
    10981 aagatgtccc agaaaacgtg gtagtagcag gtgttccagc tcgtattatc aaagaaattg
    11041 atgcccaaac tcaacaaaaa acagcgctag aggatgcgct tcgtaccttg taattgtaaa
    11101 agtaaaaaag aggcggaacc ctttttccag cctctttctg ctatatcgga ggacagatag
    11161 atgttagatt tgattcagac tagacgagat ttacaccaga taccagagat tggcttggag
    11221 gagttcaaga ctcaggctta tttgctggat gtgattgaga aattgactac gggcaaggat
    11281 tttgttcaaa ttcgtacttg gcggacaggg attttggtct acctgcaggg aagtcagccg
    11341 gagcgaacca ttggttggcg gacagatatt gatggcctgc ctatcgtcga acaaacaggt
    11401 ctaccttttg cttctcaaca ccaaggtcgc atgcatgcct gtggtcatga ttttcatatg
    11461 accattgcct taggctgtct tgagcgcgcc cttgaggagc aacccaagaa taatctgctc
    11521 tttctatttc agcctgctga agaaaatgaa gctggtggta tgctcatgta tgaggacggt
    11581 gcttttggag attggttgcc aaaccaattc tatggtctcc atgttcgtcc agatttgaaa
    11641 gtcggccaga ttgcgactaa tactcataca ctctttgcag gaacttgcga ggtgaagatc
    11701 cgtttcaaag gcaaaggtgg ccacgcagct tttcctcatg aagccaatga cgccttggtg
    11761 gcggctagtt actttgtgac ccaagtgcag tcagttgtta gccgcaatgt caacccaatc
    11821 gagggagcag tggtgacctt cggcgttttt caagctggaa ccaccaacaa tgtcatcaca
    11881 gacacagctt ttttacatgg aactattcgt gccttgacac aggacatgag tctcttggtg
    11941 caaaagaggg taaaaacagt tgcagaaggg gttgcagctg cctttgatat ggaagtcgaa
    12001 gtggaactca aacaaggtgg atacctacct gttgagaaca atccagcctt ggcgcgtgaa
    12061 ctgatggact tctttgacga aaaagacgga atcgagttga ttgatatcga acctgctatg
    12121 acaggcgagg actttggtta tctcctttca aaggttgatg gcgtgatgtt ttggctaggt
    12181 atcgatagtc cctacgctct tcatcaccct caaatgagtc ctaaggaaga agtcttagct
    12241 attggggttg atgcagtctc tagtttcctg aaaaagaagg cagcagaata gagggcttgt
    12301 atatgaaatc ggaattacgc aagcaagtct tgcatgaaat gaaggcttta tctcaagaac
    12361 aaaaacaggc tatagaccaa gctttaaccg agcgaatttt acaacacccc ttttatcaag
    12421 aagccaaggt catcgcaacc tacctctctt tttctcatga gtttcaaacg cgggaactga
    12481 ttgagcaggc gctgaaggac ggcaagaagg ttttaatacc caaaacttat cccaaggggc
    12541 gcatggactt tgtagtctat gatccgcagc agttggtaaa aactgccttt gccttactgg
    12601 agccacaggg agatttggaa gtggtggatg tatctcagat tgatttgatt catgttcctg
    12661 gcctggcttt tacgacggat ggatatcgga ttggatatgg tggaggttat tatgaccgct
    12721 atctggaaca tttttctggt catactttga gtacggttca tccttgtcaa attcaggact
    12781 ttatacctga aaaccatgat attcctgttc aggaggtatt aattgatgaa ggaaatcttt
    12841 gatagacgtt accctgtgac gagtttcttc ctcttagtga cggccttggt atttttacta
    12901 atgttggtca ctgcaggcgg aaactttgac agggcagata cattatttcg atttggagcc
    12961 atgtatgggc cagctattcg cctctttccc gagcaggttt ggcgtctctt gtctgccatt
    13021 tttgttcata ttgggtggga acatttcatt gttaatatgc tttcacttta ttatcttgga
    13081 aggcaggtag aggagatttt cggctctaag cagttctttt ttctctatct cttatcagga
    13141 atgatgggca atctctttgt ttttgtattt agtcctaaat ccttagcagc aggcgcctct
    13201 acctctcttt atgggctatt tgccgcgatt atcattcttc gctatgcaac tcgcaatcct
    13261 tatatccaac agctagggca atcctatctg acactttttg tggttaacat tattggaagt
    13321 gttctgattc caggaatcag cctagcaggc catatcggtg gtgcagttgg tggcgcattt
    13381 ctagcagtta tctttccagt tagaggagaa aaacggatgt ataacaccag ccagagatta
    13441 ggagcggtag tcttgttcgt aggactcgcc attttgcttt tctacaaggg aatgggaatg
    13501 tgacgaagtt gaaagaaaat ttgtgtaatg aaaaaagata aggcattttt tgaaccttat
    13561 ctttttattt tattccttct cagtcaattc ttttccaagt tggatgaggt aattcttcaa
    13621 atcatctttg acttgtggat gtttgagggc gtagtcaata gatgttttca taaagccaaa
    13681 cttatcccca acgtcgtaac gagctcctgt aaattcacgg gcaaaaacac gctgtgtttt
    13741 attaagagta tcgattgcat cagtaagttg gatttcattt ccagcaccag gagcttggtt
    13801 ttcgaggatt tggaaaattt caggcgtgag gaggtagcgt ccgataatag caaggtcgct
    13861 aggagcgtcc tctggagctg gtttttcaac aaaggtttca acactgtaaa gaccatcttt
    13921 tccttcgcct tgcggagcaa taaccccgta agcagatact tcgtcatgag ggactggcat
    13981 gacagcgata gtagacgcgt gggtacgctt gtagtcatcc atgagttgtt tggtaagtgg
    14041 aacagccttt tcgtctgtga tatccatcaa gtcatcacca agcataacga caaaaggttc
    14101 atttccgacg aaagccttgg cttgcaaaac agcatctccg agaccgcgtg gatgagtttg
    14161 gcggataaaa tgcagacgca tgccagttgt ttcatcaact agcttcaaaa gatctgtttt
    14221 ccctttttct ttgaggttat attccaattc gaagtttgaa tcgaagtggt cctcaataga
    14281 acgttttgat ttaccagtga caactagaat atcttcaata cctgatttga gagcctcttc
    14341 cacgataaac tggatagttg gtttgtctac gattggcaac atttctttgg caagggcctt
    14401 ggttgctggt aaaaatcgag ttcctagtcc agcagcaggg ataactgcct ttctaacttt
    14461 tgatgtcata aaaatccttt ctatagaggg ttaagaccac tcattttctg ctttaaattc
    14521 attgttcatg atgtcataaa tggcatcttt gatattggtt ccgtgataaa taacttggta
    14581 aatagcctgt gtaatgggca tatagactcc aagttcttgg gctagttcat aggctgctcg
    14641 agtcgttgaa attccttcga ttaccatgcc catattagct tctatatcag ctagggattc
    14701 tcctcgtccg agagcatctc cagctctcca gttacgagag tggatggaag ttcccgttac
    14761 gatcaaatct cccacaccag ataagccgct ataggtcaat ggactggccc cgagtgctac
    14821 ccctaggcgg gtgatttctg ctaaacctcg agcgatgatg gctgccttag cattatcacc
    14881 aaatccaaga ccatgtaaag ctccagcacc gacagcaata atatttttaa gagcaccagc
    14941 agtttcaacc ccgataacat ccgtattggt ataaagtcgg aagtagtgat tactaaatag
    15001 cttctgaacg tattgagctg tttgtaaatc tttagaagca gcagttatta aagttaggtc
    15061 acgcacaatg gtctcttctg catgactagg ccctgaaaca acgacgatat cactacggag
    15121 atgttcagga atttcttctt caagaatggt tgataatcgt ttatggctat caggttctaa
    15181 tccctttgat gcgtgcatga tgataacctt atggtccaag gtttgtgcaa cttgctgggc
    15241 aacaagtcgt gtcacttttg ttgggacaac aaacaaaatc gcatccacat ctttcaatgt
    15301 ttctgctaag tcggtgtagg caatgatatt ttcgtctaga acgacatctt taaagtagtg
    15361 cttattagta tggtgtgtat taatttcatt gatttgctcg ggaagatttc cccaaatacg
    15421 tacctcgtgt ccattgtcat ttaagacttg tgaaagggcg gttccccaag aaccaggccc
    15481 caagacggcg acggtttgtt tttccatgat ttcctccttg atagagttct ttctcttatt
    15541 ctatcatatt ctagaggatt ttgcacttgc attactgggg tatggtggaa aaatacacaa
    15601 aaaccgagac gaagggataa actcttagtc tttagttaaa gtgagaagaa atctgataga
    15661 tagtttgatt cagtatatgc ttataataga ggtaaacaac tcaggaggtt cttgtaggtt
    15721 gcgagagttt ggcgaaaaaa ttaaaagatt acgtttggct aaaaaaatca gtcgttcaga
    15781 attttgtggt gatgagtctg aattaagtat ccgtcaatta attagaattg aaaatggaga
    15841 atccagacca atactaacaa agttaaaata tattgctgaa cgtttggggg ttgaagatta
    15901 caagttgatg ccaagttata tagagttgga taaggaatac ctagaattga agtatttctt
    15961 gatgaggatt cctacatacg aagatgaaac tatcgcccaa aagaaagaga gtgttttgat
    16021 aagatttttg aagagtatta tgataggtta cctgaggaag aaagatttat catcccaaat
    16081 tattcatatc tggcactaac gaactacaca gttcaaaaat taccagaaaa gctagttgaa
    16141 atactgtcct tctggtgatt ttgcttaatt tctgatgttc gagacataat tgaggagatt
    16201 tgtcttgatt ttttcctaaa aaagggcaga ctcctccctt ggtttgatgc tcgattttta
    16261 aaataaggtg acttattctg ttaggcatgt tttcccctgc cctctagctc tatatggaac
    16321 agggagtatc agatttatct ggcagattgt atcaaataag gcttgaaact gttttgaata
    16381 ggcgtataga cttgagaatt tgagaatatg tcgtctagca gtagagacat attttccggc
    16441 aatatgaagg aagagacgtc gaaaacgctt gatagtcaag gcctttactt catcaccagc
    16501 tagctgcttt aaaaagaggt agagattata agccagacag cccatcatca tacgtacttc
    16561 attcttaatc atggtcgaac tatctgtctt atccccaaag aatcctgctt tcctttcttt
    16621 gataaagttt tcagcattcc ctcgttcacg atagaattga aacagctcct cactatctac
    16681 actgcttaaa ttgttcaaat gaactttttt acaaccataa aaaagagcac ttaatcgtac
    16741 tcttctcttt taaggattag ttgaattaat tcaaatccac cagttctcct gtttctaggt
    16801 agactaggcg ttcacaaatg ttagcaatgt aatcagcgaa gcgttccaga tgccctatga
    16861 tataaaggta ttgagttcca ttgggaattg aggtttcttg gtctttcata agtccaatga
    16921 tttcctttga taaggcataa taatattggt caatctgttc atctttttga gcaatactaa
    16981 tagcttttga ggcttggtgc aaaggaaagg caaccaataa atcggctagc atgctgaggg
    17041 ataatttacc catttggtgt aactgttctt cgtcaggggc tagttgattt tcttttagtt
    17101 gcaaaacagc tttggcaatg cctgccatat ggtctcccat acgttcaagg tctgaacaag
    17161 aagacatgat gctaattaca aatcgaaggt cagacacttg tggctgctgc aaggctaaca
    17221 aacgggcaca ggccaattcg atagcgcttt gaccttggtt gatagcatga tccttattga
    17281 taattagctc tgccatctcc ttgtctttgg aggctaaggc cagtaaggct tttgaagctg
    17341 tttcaaggac aagttgccct agtcctaaaa aggattgttc taattcatgc aattctaagt
    17401 caaattgatt tctcataggt ttttccttcc ttatccgaac cgtcctgaaa tatagtcttc
    17461 tgtgcgctga tcttttggat tggtaaacac gtcaacggta tctccaaatt cgcaaatttc
    17521 tcctgttaag aaaaaagcag ttttatctga aatacgtgaa gcttgttgca tgttatgggt
    17581 aacaatgata atcgtataat ccttttttag ttgctgaatg aggtcttcaa tttttaaagt
    17641 ggagataggg tctaaggctg aagtcggctc atccattaac agaatatcag gttctactgc
    17701 taaagctcgc gcaatgcaaa ggcgttgctg ctgaccgcca gataaggaca tggcactctt
    17761 tttaagatca tctttgactt cttcccaaat ggctgcccct tttaaagatt tctccactaa
    17821 ggcatctaat tgttttttgt ctcgaatacc atgtgtcctt gggccataag ccacgttatc
    17881 atagatagac atggcaaagg gattaggctg ttgaaaaacc atccctacac gcttacgtag
    17941 ctgattaagg ttgaatttgc tactataaat atcttgctca tctaagagga cttggccttc
    18001 aatatggcaa gaaggaacca aatcgttcat ccgattaagg gtttttagaa aagttgattt
    18061 accacaacca gatgggccta tcaaggcagt aatctgtctt tctggtaatt gaatcgaaat
    18121 attttttaag gcttgaaaat ccccgtaaaa taagtctagg tgtctgactg aaaatgttcc
    18181 catactagga agctcctttc acaagtttcc gagataataa gcttgataga gtatttatca
    18241 ttaaaacagt aataatcaaa atcacaccgg tagcataggc ttcattgaca tgtagcccct
    18301 cacttgacag catatacata tgcagggcta gagaacgacc tgaagacatg agactacttg
    18361 gcgtattggt agaggtacct aatgtataca tgagggcagc tgtttcacca acgatacggc
    18421 caatagctag tatcactcca gctaaaatac ctggcatggc aactggtaga acaattctaa
    18481 aaacagtccg taacttacct gccccaagtc cataacttgc ttgacgcatg ctatcactaa
    18541 cagataaaag ggcttcttct gttgagcgaa taatgactgg caacaccatg ataactgagg
    18601 ttaagattcc tgataacaga gagtattgaa aacctaagaa gactacaaag aagagcatgc
    18661 caaacagacc aaaaacaatg gaaggaatcc cagataaggt atctgaggcc aatcgcatga
    18721 ttttaacaca aagggaatct ttttttgtat attccacaag ataaaaacca gcaaaaatcc
    18781 ctatgggcaa ggctaaaaga agagcaccaa agaccagaat aacggtggaa ataatcgctg
    18841 gcataaggga aatgttctca gaagtataag tccaagaaaa gagggataga cttagatgag
    18901 gtaagccttt gatgaggata aaaccaatga ctaaaaagag agagccaaag gttaaagctg
    18961 aaaaacaata aacgagaagt tttagcaggt atttactcat aagatgattt tcctttcaag
    19021 taggcaaagt aggcattaat caagagaata aggaaaaaga gaactgctga ggttgcaata
    19081 agggcttccc tatgctgacc tgatgcgtaa gccatttcca gaacaatatt ggttgttaag
    19141 gttctggttc ctgaaaagag tccacttgga ataatcggtt ggttgcctgc taccaaaata
    19201 actgccatgg tttcacctac tgcgcgaccg attcctaaaa taactgctga taaaatacca
    19261 gatctcgcag ctggcaagat gacactaaaa atactccgtt catgactagc tcctagagcc
    19321 aagctaccag aataatacgt tttgggaact gttcggatag cagattctga caaactgata
    19381 atggttggca aaatcattat tcctaatagt aacgaagcgg ttaggacact catgccattt
    19441 cctaaaaagc ttctaatcca aggcaccaat aattgtaggc cgaaaaaacc ataaacaata
    19501 gatggaatgg ccgccatcaa gttgatagct gattttaaga agccatagac gggctttgga
    19561 caataataaa ccataaacac cgatgtcaag atgcctgttg gcaccccaat cacaatcgct
    19621 cctaaggtaa ttaataagga accaacgatc attggtaaaa taccatagct tgccggaatg
    19681 ttcgttggcg accaatcact gcctaataaa aaacgggcaa agccgtagtt agccatgaaa
    19741 ggtaagccat tactaaaaat aaagaaacag attagcaaaa tagctacaac agctactgtt
    19801 gcactcatga aaaaaattgc cctaaaaact gcttctttga aggcttgttt tatcacatct
    19861 tgtcctttct agtgaagaaa gtaagggaga tacgacacct ccctacttgc cttctttatc
    19921 ttattgtacg atgaaacgtc tgcatctctt tagagattta tggagcaaac attttattta
    19981 atcttgtccc aggtggttaa tttgccacta aaaacgtctg caagttcagc catactgact
    20041 tggcttgcct tattgtcatt attgaccaca acagcaatac cgtctaaagc aatagcatca
    20101 tgggtgagac tcttaccttc ttcaggagtt aattccctag aaaccatacc aatatcagcg
    20161 gttttctcct taacagcggt aatacctgct gaagacccat tagaggtaat atcaatcgta
    20221 acttctggat tttctttttt ataagcttct gctaattttt ccattaaaga agatactgaa
    20281 gtggaaccta caacagacaa cttgcctgat aagtgttggc tggtatattc tgtggtttcg
    20341 gttttagctt caataaattt attatctgtg accacttgtt gaccttgttt ggagtggata
    20401 aagctgataa aatcttgacc tagcttggaa agattagaag accaaacaat gttgaaggga
    20461 cgttgaagag ggtatttacc atctaaaact gtgtctcgac tagccttgac accatcaatc
    20521 tctaaagcct tgacagattt cgttaaagat cccaaggaga tgtagccgat agcattagca
    20581 ttcccttgaa ctgctgagag aacaccttct gtactatttt gaatcacagc tgttttggca
    20641 gtgttgtcaa tttttttatc accgtctttt ttgagaatcc ctgtgatttc tgtgaaggca
    20701 ccccgtgtcc cagagccatt ttctcgtgaa atcacctcaa tcgttcctga agctgactgt
    20761 ttggaagcag ctgactgatt gccacaggca acaagcccaa atcctgataa gccaatggct
    20821 gcaagagtaa gcattttttt gaatttcata ataatcacct ttatctctat gtatttttct
    20881 tgtgtaggct tactacattt atagtctaac aagtctttgt aaaggtttat ccctgattca
    20941 tgtaaagatt gtgtaaagaa tcaaaaaaag ccacttttga aaaatggctg ctcctaaaaa
    21001 tagctttaaa aattattagt cctgtgcgaa agattggtta ggaagaaaaa tcgtgaagca
    21061 actgcctctg ccaagctgac tcgtcaccgt gacttggcca cctaataatt gactgagttc
    21121 tttgacaatg gcaaggccaa gaccagtgcc accagtttgt ctgcttcgac ctttattaac
    21181 tcggtaaaaa cgttcaaaaa tacgatcctg ctctaattga ctaataccaa tccctgtatc
    21241 tgatacagaa atcttaatgc cttcgttcac cttttgggtc ttgacctcaa tttttccccc
    21301 ttgttcagtg taacggatgg cattggataa aagattgagt aagatttggg aaagtaattg
    21361 actatctgat acgagggtga catcatctgg cacctgcacc tttagctgta aatccttctt
    21421 cttgagctga ggttgcaagc tttgagtcaa atcctgtaca aattctgcca aagaaagggt
    21481 cgtccattgt ataggcattt gttgagcctt agataaggta agaagatgct caacaatatg
    21541 ctcaagacgc aagctttctt tgtaaataat gtctagaaag tcatccttga gcgcttcttc
    21601 ttcagctgac atccccttaa tggtttcagc aaagccctta atcgaagtaa ctggtgtcct
    21661 caattcatgg gaggcatttg agacaaaggc taaatttaac ttttcataag ttctaatcgt
    21721 tgttaaatca tatagcaaga cgagcacagc ttccacagat tgggtggggc taaaaacggg
    21781 aactgctgtc acttctaaaa tcaagtcacc ctcatgaaac ccacttactt cttgttttaa
    21841 ccttgttttt tgatcaaagg cttggtgaac taaatttcga atatccatcc gtttgaggtc
    21901 atcaagtgaa cttatgtcgc cgtccacatc gggaaaataa tgaggcagag agcgactgga
    21961 taataacatc tgaccttgag cggaaactaa aaacgtcccc atggttaggt gcgacagaag
    22021 aacctccatt gtttcggcta ggtccttgta ttgctgatcc tgttgggaga ctttggtttt
    22081 tagggcagac acatactgag ccaaagactt taagtcttct tgcccttttt ctaaaaagta
    22141 ttcactactg gtcaagagag gttggtgcaa ggtctcaaaa gcaacttccc atttccaaag
    22201 gcaaaagagc cagtagccac cttgtcccaa agaaagggct agaagaaaga gaccgatgcc
    22261 tttactgatc caagttaatg ccatccctgc aatcagaatg aggctaacac ttagattgac
    22321 tagccaaaat tgaaggtagc gtttcatcta taactccttg aacttataac cataaccccg
    22381 aatggttcga ataaattgag gggctttagg attgtcttca attttttccc tcaacttacc
    22441 aatatgaacg tccaccaaac gtgtttcctg cccaaagtca tacccccaga tacgttccaa
    22501 aagacgctct ctagtcagtg tcatgttggg atgtttcata agatagagca agagttcaaa
    22561 ttcttttggg gtcaaactca gtaacttatt cgccttgtag acttcatgac gctcagggta
    22621 tactttcaag gtcccaaata gccaagaatc gtcagcgata ttatctgaat catctccttc
    22681 ttgttctcct ttagttcgcc tgaggacagc cttgacacgc gccagcaatt ctctagggct
    22741 aaaaggcttg gtcaggtagt catcagcccc taattccaag gccaaaacct tatcaaattc
    22801 atcacttttc gcagaaacca tcataattgg agttttgacg cctttggctc tcagccgctt
    22861 acaaacttcc atgccatcta attgtggtaa catgatatca agcaagataa aatcaaaggg
    22921 ttctgtttct gccaaagcta aggccttccg tccatttgtt accaattgag tagaaaagcc
    22981 ttccttactt aaatggtagt caagcaattt cagaatgtgt tcttcatcat ccactaataa
    23041 gacttgtttt gtcatctatt atctcctatt ggtaacatta taacacaatt atcagaaatc
    23101 ctaacattgc taaatcagat taaatttgct tatcaagact agtatctggt caaacgctca
    23161 atcatctcct tgtgctctgg ataggtcgcc agtagatcta ccctttcaaa taattcaaaa
    23221 tcctcaaatt caaaaccagg agcaacaaga caagaaacca gagcataatc cttatcaact
    23281 gttgatcccc aaatagtgcc cttaggaaca cagtagtgaa gttgttgccc tttggatatg
    23341 tccaggccta aagtgactgc ttcgtagtga ccatctgctg taatcatgtg aacagtaagt
    23401 ggggatcctg catgaaaata ccagatttca tctgctgtca atcggtgaaa atgtgaagga
    23461 ttcgtttctt cttaataaga aataaatact ggtataaagc gcccttccct taccagcaag
    23521 gtttatagtg tctgaagctt tttttgtttg tctaaaatag ccaccttcaa catggggagc
    23581 taactctaga gttcttatca agtcttcttt atccgtcgga gccaatgggt tgaagtaact
    23641 cttgttcaaa gtggtgttac gatttcaaga actcctctca gttctgagga cacggtaatg
    23701 attgatgcga cggaagtaca aatcaatcgc cctaaaaaaa gaattagcga attattctgg
    23761 taaaaagaaa tttcacgtta tgaaggctca agcgattgtc acaagtcaag ggagaattgt
    23821 ttctttggat atcgctgtga actattgtca tgatatgaag ttgttcaaaa tgagtcgcag
    23881 aaatatcgga caagctggta aaatcttggc tgacagtggt tatcaagggc tcatgaagat
    23941 atatcctcaa gcacaaactc cacgtaaatc cagcaaactc aagccactaa cagttgaaga
    24001 taaagcctat aaccatgcgc tatccaagga gagaagcaag gtcgagaaca tctttgccaa
    24061 agtaaaaacg tttaaaatgt tttcaacaac ctatcgaaat catcgtaaac gcttcggatt
    24121 acgaatgaat ttgattgctg gtcttatcaa tcatgaacta ggattctagt tttgcaggaa
    24181 gtctattatt tggttaggtg aattagtgaa gcgtttaggc aagtgtctct ggttacgacg
    24241 taaagaagca taaatcgata atatttatgt ttctttacta gtgaaacgcc tagccaaatt
    24301 accatagtta cgacgtcatg gcctctaaat cgattatatt taggggtcat gactagtgaa
    24361 gcagttagct agttcgcata taagcggcta gcgtctaaca attaggaact ttagttccaa
    24421 taactttaag attacgacgt tttaggacat aaatcgatca tatttatgtc ctaaaactag
    24481 tgaagcgcct agccaaagtc cgaataggat ttggcgttag ttacttagat tgctttgcaa
    24541 tcaagtaact ttggcgattt acatcttctc tggcgcttct actccaagca agcgaagggc
    24601 ttctttgaga acgactgcgg ttgcgtagct gagggctaga cggctgtcgc gttctgggct
    24661 ttcatccaag atacgtgtat gtgcatagta tttgttaaag gattgagcca ggctaattgc
    24721 aaatttagca atgatagaag gttcaaagtt atctgccgca cggttgataa tacgtgggaa
    24781 gtcttgaatg agtttaatga tttcccagct ttcagtatca ttcaagctat agttgccagc
    24841 tgtttctggt ttgaaatcgg ctttacgtaa gatagattgg atacgagcgt aggcatattg
    24901 aacgtaaggt ccagtttcac cctcgaagga taccatagct tctaggtcga agtcgtatcc
    24961 atttgtacgg tcggttttga ggtcatagaa tttaatggct ccaaccccaa cagcatgtgc
    25021 tacctgatct ttattttcca attcaggatt ttttgcttca atttgggctt tggcacggct
    25081 aacagcctct gcaacagtag gctctagcaa gatgacattc cctttacgag tagagagttt
    25141 cttcccttct tttgtaacca gaccaaaagg aacgtgagta atgtcgtcac tccagtcgta
    25201 gcccatctct tgcaagacag ctttgagctg tttaaagtgg gcagattgtt cttgaccaac
    25261 gacatagata gatttagcaa attggtattc gtttttacgg taaagggctg cagccaagtc
    25321 acgtgtgata tagagagttg caccatcaga cttcttgatg agggcgggat gttcaattcc
    25381 atatttctca aggttcacaa cttgggcacc ttctgattca agaagtagtc ctttttcaga
    25441 aagaatgtct acaactgcat ccatcttatc attgtagaag gcttctccgt tatagctgtc
    25501 aaattcaacc ttcaattcat tgtaaaggcg gttaaattcc actaaacttt catcgcggaa
    25561 ccattgccaa agagcgagag cttcctcatc tccattttca agtttacgga accattcgcg
    25621 cgcttcttca tccaagctag ggtcattttc agcttcagcg ttgatgcgga catagagttt
    25681 aaggagttca tcgattggat gagcttttac agcttcttcg tcgccccatt ttttgtaggc
    25741 aacaatcaac atcccaaatt gtttacccca gtctcccaaa tggttgacct tgaccgtttg
    25801 ataaccgatt ttttggaaaa tatgtgacaa gctatctccg ataacagttg aacgcaggtg
    25861 gccaatagaa aatggtttag cgatattcgg actagacatg tcgataacaa cattttcttg
    25921 tttaccaata ttttggtcag catagtgttc tttttcagtg gtaacagctt gcaatacttg
    25981 agcagaaatg gcagatttat caaggaaaaa gttaacgtaa ggtcctgttg cgacaacctt
    26041 ttcaaaggct tggctgttca ttttttcagc cagttcagtc gcaatcattt gaggtgcttt
    26101 acgttcgact tttgcaagag aaaaagcagg gaaagcgatg tctcccattt ctgagttttt
    26161 aggggtttcc agtaaattta aaatagcctc ttggtccagg ctatcaatga tgctagataa
    26221 ttcgctagca atcaattctt ttgtattcat taagagctcc tttttggact tttctactat
    26281 tttatcacaa ttttaaagaa agaagaaaaa atttttgaaa tctcccgttt ttttggtata
    26341 atatagctat aaatatagtt ataaatatac atataagagg attttatgag aaaaagagat
    26401 cgtcatcagt taataaaaaa aatgattact gaggagaaat taagtacaca aaaagaaatt
    26461 caagatcggt tggaggcgca caatgtttgt gtgacgcaga caaccttgtc tcgtgatttg
    26521 cgcgaaatcg gcttgaccaa ggtcaagaaa aatgatatgg tgtattatgt actagcaaat
    26581 gagacagaaa agattgattt ggtggaattt ttgtctcatc atttagaagg tgttgcaaga
    26641 gcagagttta ccttggtgct tcataccaaa ttgggagaag cctctgtttt ggcaaatatt
    26701 gtagatgtaa acaaggatga atggatttta ggaacagttg ctggtgccaa taccttattg
    26761 gttatttgtc gagatcagca cgttgccaaa ctcatggaag atcgtttgct agatttgatg
    26821 aaagataagt aaggtcttgg gagttgctct caagacttat ttttgaaaag gagagacaga
    26881 aaatggcgat agaaaagcta tcacccggca tgcaacagta tgtggatatt aaaaagcaat
    26941 atccagatgc ttttttgctc tttcgtatgg gtgattttta tgaattattt tatgaggatg
    27001 cggtcaatgc tgcgcagatt ctggaaattt ccttaacgag tcgcaacaag aatgccgaca
    27061 atccgatccc tatggcgggt gttccctatc attctgccca acagtatatc gatgtcttga
    27121 ttgagcaggg ttataaggtg gctatcgcag agcagatgga agatcctaaa caagcagttg
    27181 gggttgttaa acgagaggtt gttcaggtca ttacgccagg gacagtggtc gatagcagta
    27241 agccggacag tcagaataat tttttggttg ccatagaccg cgaaggcaat caatttggcc
    27301 tagcttatat ggatttggtg acgggtgact tttatgtgac aggtcttttg gatttcacgc
    27361 tggtttgtgg ggaaatccgt aacctcaagg ctcgagaagt ggtgttgggt tatgacttgt
    27421 ctgaggaaga agaacaaatc ctcagctgcc agatgaatct ggtactctct tatgaaaaag
    27481 aaagctttga agaccttcat ttattggatt tgcgattggc aacggtggag caaacggcat
    27541 ctagtaagct gctccagtat gttcatcgga ctcagatgag ggaattgaac cacctcaaac
    27601 ctgttatccg atacgaaatt aaggatttct tgcagatgga ttatgcgacc aaggctagtc
    27661 tggatttggt tgagaatgct cgctcaggta agaaacaagg cagtcttttc tggcttttgg
    27721 atgaaaccaa aacggctatg gggatgcgtc tcttgcgttc ttggattcat cgccccttga
    27781 ttgataagga acgaatcgtc caacgtcaag aagtagtgca ggtctttctc gaccatttct
    27841 ttgagcgtag tgacttgaca gacagtctca agggtgttta tgacattgag cgcttggcta
    27901 gtcgtgtttc ttttggcaaa accaatccaa aggatctctt gcagttggcg actaccttgt
    27961 ctagtgtgcc acggattcgt gagattttag aagggatgga gcaacctgct ctagcctatc
    28021 tcatcgcaca actggatgca atccctgagt tggagagttt gattagcgca gcgattgctc
    28081 ctgaagctcc tcatgtgatt acagatgggg gaattatccg gactggattt gatgagactt
    28141 tagacaagta tcgttgcgtt ctcagagaag ggactagctg gattgctgag attgaggcta
    28201 aggagcgaga aaactctggt atcagcacgc tcaagattga ctacaataaa aaggatggct
    28261 actattttca tgtgaccaat tcgcaactgg gaaatgtgcc agcccacttt ttccgcaagg
    28321 cgacgctgaa aaactcagaa cgctttggaa ccgaagaatt agcccgtatc gagggagata
    28381 tgcttgaggc gcgtgaggag tcagccaacc tcgaatacga aatatttatg cgcattcgtg
    28441 aagaggtcgg caagtacatc cagcgtttac aatctctagc ccaaggaatt gcgacggttg
    28501 atgtcttaca gagtctggcg gttgtggctg aaacccagca tttgattcga cctgagtttg
    28561 gtgacgattc acaaattgat atccagaaag ggcgccatgc tgtcgttgaa aaggttatgg
    28621 gggctcagac ctatattcca aatacgattc agatggcaga agataccagt attcaactga
    28681 ttacagggcc aaacatgagt gggaagtcta cctatatgcg tcagttagcc atgacggcgg
    28741 ttatggccca gctgggttcc tatgttcctg ctgaaaccgc ccatttaccg atttttgatg
    28801 cgatttttac ccgtatcgga gcagcagatg acttggtttc gggtcagtca acctttatgg
    28861 tggagatgat ggaggccaat aatgccattt cgcatgcgac caagaactct ctcattctat
    28921 ttgatgaatt gggacgtgga actgcaactt atgacgggat ggctcttgct cagtccatca
    28981 tcgaatatat ccatgagcac atcggagcta agaccctctt tgcgacccac taccatgagt
    29041 tgactagtct ggagtctagt ttacaacact tggtcaatgt ccacgtggca actttggagc
    29101 aggatgggca ggtcaccttc cttcacaaga ttgaatcggg accagctgat aaatcctacg
    29161 gtatccatgt tgccaagatt gctggcttgc cagcagacct tttagcaagg gcggataaga
    29221 ttttgactca gctagagaat caaggaacag agagtcctcc tcccatgaga caaactagtg
    29281 ctgtcactga acagatttca ctctttgata gggcagaaga gcatcctatc ctagcagaat
    29341 tagctaaact ggatgtgtat aatatgacac ctatgcaggt tatgaatgtc ttagtagagt
    29401 taaaacagaa actataaaac caagactcac tagttaatct agctgtatca aggagacttc
    29461 tttgacaatt ctccactttt ttgctagaat aacatcacac aaacagaatg aaaaggagct
    29521 gacgcattgt cgctcccttt tgtctatttt ttaaggagaa agtatgctga ttcagaaaat
    29581 aaaaacctac aagtggcagg ccctggcttc gctcctgatg acaggcttga tggttgctag
    29641 ttcacttctg caaccgcgtt atttgcagga agtcttagac gccctcctta ctgggaaata
    29701 tgaagctatt tatagtatcg gggcttggtt gattggtgtg gccgtagtcg gtctagttgc
    29761 tggtggactc aatgttgtcc tcgcagccta tattgcccaa ggagtttcat ccgaccttcg
    29821 ggaggatgcc ttccgtaaaa ttcaaacctt ttcttatgct gatattgaac aatttaatgc
    29881 gggaaatcta gtcgttcgaa tgacaaatga tatcaaccag attcagaacg ttgtcatgat
    29941 gaccttccaa attcttttca gacttcccct cttgttcatc ggttcgttta tcctagcggt
    30001 tcaaacctta ccttctctgt ggtgggtgat tgttctcatg gtagtcttga tttttggttt
    30061 gactgctgtc atgatgggaa tgatggggcc tcgttttgct aagtttcaaa cccttcttga
    30121 gcgcatcaat gccattgcca aggaaaattt acgtggcgtt cgtgtggtca agtcctttgt
    30181 ccaagaaaaa gagcaatttg ctaagtttac agaggtctca gacgagcttc ttggtcaaaa
    30241 cttttacatt ggttatgcct tttcagtagt ggaacccttt atgatgttgg ttggttacgg
    30301 ggcggtcttc ctctctattt ggctggtcgc gggaatggtt cagtcggatc cgtctgttgt
    30361 tggttccatc gcttcttttg ttaattacct aagccagact atctttacca ttgttatggt
    30421 tggatttttg ggaaattctg tcagccgtgc catgatttcc atgcgtcgta ttcgagaaat
    30481 tcttgacgca gagccagcta tgaccttcaa ggatatccca gatgaagagt tggttggaag
    30541 ccttagcttt gaaaatgtga cctttaccta tccaatggac aaggaaccga tgctgaaaga
    30601 tgtgagcttt actattgaac ctggtcaaat ggttggtgta gttggagcga ctggtgcagg
    30661 aaagtcaacc ttggctcaat tgattccacg tctctttgat ccacaggacg gggccgttaa
    30721 aatcggtggc aaggatattc gagaagtgag tgaaggaacc ctgcgtaaaa cagtttccat
    30781 cgttctccaa cgtgccattc tttttagtgg aacgattgca gataacttga gacaggggaa
    30841 gggaaatgct actctatttg aaatggagcg tgcagccaat attgcccagg ctagtgaatt
    30901 cattcatcgt atggagaaaa cctttgaaag tccagttgaa gaacggggaa ccaatttctc
    30961 tggtggacaa aaacaaagga tgtcgattgc gcgtgggatt gtcagcaatc cacgtattct
    31021 gatttttgac gattcgacct cagccttgga tgccaagtca gagcgcttgg tgcaagaagc
    31081 tttgaataag gacttgaagg ggacgacaac cattattatt gctcaaaaaa ttagctcggt
    31141 tgtccatgca gacaagatct tggttctaaa tcaaggacga ttgattggtc aaggcacgca
    31201 tgcagacttg gttgccaaca atgccgttta ccgtgaaatc tatgaaacac agaaatgaaa
    31261 gacaaactat aagaaaagtc aatagtttta tctaaactat ttcttatttc aatttgatga
    31321 tttggcgatg attttagagc acggcaaaaa gcccttgaaa aagtccattt ttcaaaggta
    31381 atcctgtgtt aatttcagaa attacatcac tttttgttcg tcaaatggca gctctttttt
    31441 aggatataaa acagggttcg gataagtttt tttgcaaggt ggatgatggt tatattgtaa
    31501 tgttttcctt attctaactt agtcttaaga taggccttaa aagtaggcga aaagcgagcg
    31561 catgctttgg caacttgtat gagtacctac cgcagatgag gggaaccccg tttgaccatt
    31621 cttcctgcta aatcaatctg acctgactga taaatagaag aatccagtcc agcgaaagct
    31681 tgtaattgag caggattatc aaaggcatga atatttcgaa tctcggctaa aatgaccgcc
    31741 cctaaacgat tcccaatccc agtaaccgtc gtgatgaccg agttgaactc agccatcaag
    31801 tcattgacac atttttccgc tttgtcaatg atcctcttgt aatgtttgat gttttcatta
    31861 cacgagataa aacgtttatg cgttatcaaa ctcattacca attgaaacaa aagctgtggt
    31921 tagagccttt cggaaattgt caagcgattg gaggaaatga actaatccac agcggcttat
    31981 tccaagtata ccacttgggc tttggcagta gctaactgcg ctaaatataa tataaggagg
    32041 agtaaaatga agacagttca atttttttgg aattatttta aggtctacaa gttctcattt
    32101 gtagttgtca tcttgatgat tgttctggcg acttttgccc aagccctctt tccagtcttc
    32161 tctggacaag cggtgacgca gctagccaat ttagttcaag cttatcaaaa tggcaatcca
    32221 gaacttgtat ggcaaagcct atcaggaatc atggtcaatc ttggtctgct ggttttggtt
    32281 ctatttattt ctagtgtaat atacatgtgt ctcatgacgc gcgtgattgc agaatcgacc
    32341 aacgagatgc gcaaaggcct ctttggtaag cttgctcagt tgacggtttc tttctttgac
    32401 cgtcgacaag atggcgatat cctgtctcat tttaccagtg atttggataa tatcctccaa
    32461 gcctttaacg aaagcttgat tcaggtcatg agcaatgttg ttttatacat tggtctgatt
    32521 cttgtcatgt tttcgagaaa tgtgacgctg gctctcatca ccattgccag caccccattg
    32581 gctttcttta tgctgatttt catcgtgaaa atggcacgca aatccaccaa cctccagcag
    32641 aaagaggtag ggaagctcaa cgcctatatg gatgagagca tctcaggcca aaaagccgtg
    32701 attgtgcaag gaattcaaga ggatatgatg gcaggatttc ttgaacaaaa tgagcgcgtg
    32761 cgcaaggcaa cctttaaagg aagaatgttc tcaggaattc ttttccctgt tatgaatggg
    32821 atgagcctgg ttaatacagc catcgtcatc tttgctggtt cggctgtgct tttgaatgat
    32881 aagtctattg aaacaagtac agccctaggt ttgattgtta tgtttgcaca attttcacag
    32941 cagtactacc agcctattat ccaagttgca gcgagttggg gaagccttca gttggccttt
    33001 actggagctg aacgaattca ggaaatgttt gatgcagagg aggaaatccg acctgaaaag
    33061 gctccaacct tcactaagtt gcaagaaagt gttgaaatca gtcatatcga tttttcatac
    33121 ttgcctgata aacctatttt gaaagatgtc agcatttctg cccctaaagg ccagatgaca
    33181 gcagttgttg ggccgacagg ttcaggaaaa acgactatta tgaacctcat caatcgcttt
    33241 tatgatgttg atgctggtgg tatttatttt gatggtaaag acattcgtgg ctatgactta
    33301 gatagtctta gaagcaaggt gggaattgta ttgcaagatt cggtcttgtt tagcggaacg
    33361 attcgagaca atatccgatt tggtgtgcca gatgctagtc aggaaatggt tgaggtagca
    33421 gcaaaagcaa cccacattca cgactatatc gaaagtttgc ctgataagta cgatactctt
    33481 attgatgatg accagagcat cttttcaaca gggcagaagc aattgatttc aatcgctcga
    33541 accctgatga cagatccaga agttctcatt ctcgatgaag caacttcaaa cgtagatacg
    33601 gtgacagaaa gcaagattca gcatgccatg gaggtggttg tagcaggtag aactagtttc
    33661 gtcattgccc accgcttgaa aaccattctc aatgcagatc agattattgt ccttaaagat
    33721 ggagaagtca ttgaacgtgg taaccaccat gaacttttga agctaggtgg cttttattca
    33781 gaactctatc acaatcaatt tgttttcgaa taagaaagaa gttgtcctat gtgggcagct
    33841 ttttcttgtc cataaaaaat gtttatcaca gccttaaaaa aaacatatta gacgaaagtc
    33901 attttgagtg atatgatagg actatcgtta gcattcgaaa ggagaggcat catggctaga
    33961 acggttgtag gagttgctgc aaatctatgt cccgtagacg cagaaggcaa aatcattcat
    34021 tcatctgtat cttgtagatt cgcagagatc attcgtcaag tcggtggtct ccctttagtc
    34081 attcctgttg gtgatgagtc agttgtacgt gattatgtgg aaatgattga caagctcatt
    34141 ttgacaggag gccaaaatgt tcatcctcag ttctatggag agaaaaagac cgtcgagagc
    34201 gatgattaca atctggtccg tgacgaattt gaattggcac tcttgaagga agcgcttcgt
    34261 cagaataaac caattatggc aatctgtcgc ggtgtccaac ttgtcaatgt tgcctttggt
    34321 ggaaccctca atcaagaaat cgaaggtcac tggcaaggac tacctttagg gacatctcac
    34381 tctattgaga cagtagaagg aagcgtggtg gctaagctat ttgggaaaga aagtcaggtc
    34441 aactcagtcc atcgtcaaag cattaaagat ttggcaccta atttccgtgt aactgctatt
    34501 gattcaagag accaaactat tgaagcgatt gagtctatcg acgagcaccg cattatcggt
    34561 ttacagtggc atccagagtt tctggttaat gaagaagatg gcaatttaga attatttgag
    34621 tatttattga atgaactgta
//
LOCUS       DAVKDZ010000015        34227 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000015 DAVKDZ010000000
VERSION     DAVKDZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34227
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.015"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            57..1949
                     /gene="pepO"
                     /locus_tag="VWZ30_000692"
     CDS             57..1949
                     /gene="pepO"
                     /locus_tag="VWZ30_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEU9334475.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(2072..2701)
                     /locus_tag="VWZ30_000693"
     CDS             complement(2072..2701)
                     /locus_tag="VWZ30_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9334476.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            3695..5917
                     /locus_tag="VWZ30_000694"
     CDS             3695..5917
                     /locus_tag="VWZ30_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU9334477.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            5947..6390
                     /locus_tag="VWZ30_000695"
     CDS             5947..6390
                     /locus_tag="VWZ30_000695"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEU9334478.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(6657..6842)
                     /locus_tag="VWZ30_000696"
     CDS             complement(6657..6842)
                     /locus_tag="VWZ30_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334479.1"
                     /translation="MKKIIFIKTIQLLVIDGIMLAFLTFKRELTWDWILIYSGWLIFF
                     HPVLLTYLSNQLCDHFS"
     gene            complement(6861..7274)
                     /locus_tag="VWZ30_000697"
     CDS             complement(6861..7274)
                     /locus_tag="VWZ30_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334480.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGILTIDFLFFVLLAFISSQRFSSLMP
                     IITARHSTFYYMTADYLGENYPDFYDKHISLWECL"
     gene            complement(7405..8051)
                     /locus_tag="VWZ30_000698"
                     /pseudo
     CDS             complement(7405..8051)
                     /locus_tag="VWZ30_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
     gene            complement(8228..8878)
                     /locus_tag="VWZ30_000699"
     CDS             complement(8228..8878)
                     /locus_tag="VWZ30_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU9334481.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            8990..9832
                     /locus_tag="VWZ30_000700"
     CDS             8990..9832
                     /locus_tag="VWZ30_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9334482.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADAQQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            9908..10345
                     /locus_tag="VWZ30_000701"
     CDS             9908..10345
                     /locus_tag="VWZ30_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU9334483.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            10414..10716
                     /locus_tag="VWZ30_000702"
     CDS             10414..10716
                     /locus_tag="VWZ30_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334484.1"
                     /translation="MYLVIEIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYSGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(10807..11880)
                     /locus_tag="VWZ30_000703"
     CDS             complement(10807..11880)
                     /locus_tag="VWZ30_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEU9334485.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFNWKIEDKHFVQLDKTNSDSRQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYHAWKNR"
     gene            12036..12713
                     /locus_tag="VWZ30_000704"
     CDS             12036..12713
                     /locus_tag="VWZ30_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9334486.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            12706..13680
                     /locus_tag="VWZ30_000705"
     CDS             12706..13680
                     /locus_tag="VWZ30_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9334487.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(13729..15672)
                     /gene="thrS"
                     /locus_tag="VWZ30_000706"
     CDS             complement(13729..15672)
                     /gene="thrS"
                     /locus_tag="VWZ30_000706"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU9334488.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(15730..15906)
                     /locus_tag="VWZ30_000707"
     CDS             complement(15730..15906)
                     /locus_tag="VWZ30_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU9334489.1"
                     /translation="MTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENF
                     LSDKEKQLNVRTRK"
     gene            complement(15913..16053)
                     /locus_tag="VWZ30_000708"
     CDS             complement(15913..16053)
                     /locus_tag="VWZ30_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU9334490.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            complement(16310..16684)
                     /locus_tag="VWZ30_000709"
     CDS             complement(16310..16684)
                     /locus_tag="VWZ30_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334491.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKGLYINNLL
                     FFTPLVILIISLLYSYRFRFSLWWLVGNGLLFYFTIITFGEFILIYLLIYETVALVSM
                     DSGISIKHILQKMKNKKLSQNP"
     gene            16923..17552
                     /locus_tag="VWZ30_000710"
     CDS             16923..17552
                     /locus_tag="VWZ30_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9334492.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQSESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGSGS
                     FYIDETKTTRLLDTSDFPEEFKPLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            17693..17962
                     /gene="rpsO"
                     /locus_tag="VWZ30_000711"
     CDS             17693..17962
                     /gene="rpsO"
                     /locus_tag="VWZ30_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU9334493.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            18777..19391
                     /locus_tag="VWZ30_000712"
     CDS             18777..19391
                     /locus_tag="VWZ30_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEU9334494.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAILGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(19684..20433)
                     /locus_tag="VWZ30_000713"
     CDS             complement(19684..20433)
                     /locus_tag="VWZ30_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU9334495.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            20566..22902
                     /locus_tag="VWZ30_000714"
     CDS             20566..22902
                     /locus_tag="VWZ30_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU9334496.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNLAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEQNFLRKSMLRALPSALMVVFSVLFVKIF
                     GSSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            23004..23681
                     /locus_tag="VWZ30_000715"
     CDS             23004..23681
                     /locus_tag="VWZ30_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEU9334497.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            23668..24465
                     /locus_tag="VWZ30_000716"
     CDS             23668..24465
                     /locus_tag="VWZ30_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU9334498.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQEDDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIATLLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            24482..25585
                     /locus_tag="VWZ30_000717"
     CDS             24482..25585
                     /locus_tag="VWZ30_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU9334499.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESNLE
                     ELYQLALQRREESPLIGQLAILNQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEVSHVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            25841..26860
                     /gene="galE"
                     /locus_tag="VWZ30_000718"
     CDS             25841..26860
                     /gene="galE"
                     /locus_tag="VWZ30_000718"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9334500.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGIIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            26862..27827
                     /locus_tag="VWZ30_000719"
     CDS             26862..27827
                     /locus_tag="VWZ30_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9334501.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLTIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(27878..28084)
                     /locus_tag="VWZ30_000720"
     CDS             complement(27878..28084)
                     /locus_tag="VWZ30_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU9334502.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDGPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            28136..28612
                     /locus_tag="VWZ30_000721"
     CDS             28136..28612
                     /locus_tag="VWZ30_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU9334503.1"
                     /translation="MAKEPWQEDIYDQEESRAGRRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSHPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            28621..29292
                     /locus_tag="VWZ30_000722"
     CDS             28621..29292
                     /locus_tag="VWZ30_000722"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU9334504.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            29423..29761
                     /locus_tag="VWZ30_000723"
     CDS             29423..29761
                     /locus_tag="VWZ30_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEU9334505.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            29764..30393
                     /locus_tag="VWZ30_000724"
     CDS             29764..30393
                     /locus_tag="VWZ30_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEU9334506.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            30633..31784
                     /locus_tag="VWZ30_000725"
     CDS             30633..31784
                     /locus_tag="VWZ30_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9334507.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVMPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            32097..32846
                     /gene="truA"
                     /locus_tag="VWZ30_000726"
     CDS             32097..32846
                     /gene="truA"
                     /locus_tag="VWZ30_000726"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU9334508.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            32836..33609
                     /locus_tag="VWZ30_000727"
     CDS             32836..33609
                     /locus_tag="VWZ30_000727"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9334509.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNVGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            33587..34048
                     /locus_tag="VWZ30_000728"
     CDS             33587..34048
                     /locus_tag="VWZ30_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9334510.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNDWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT         9842 a         6465 c         7853 g        10067 t
ORIGIN      
        1 attccctagt gatttttgtt aagataaatg caaatacaaa tgaaagcgag aacaagatga
       61 cacgttatca agatgatttt tatgatgcta tcaatggaga atggcaacag acagctgaaa
      121 tcccagcaga taagtctcaa acaggaggtt ttgttgattt agaccaggaa attgaagacc
      181 tgatgctggc gacaacagac aagtggttag caggtgaaga agtgcctgag gatgctatct
      241 tggaaaactt tgtcaaatac caccgcctag ttcgtgattt tgacaagaga gaagctgacg
      301 gtatcacacc tgtcttacca ctccttaaag aattccaaga attggagact tttgcggatt
      361 ttacagctaa actagcagag tttgagcttg caggaaaacc aaacttcctt ccttttggtg
      421 tatcgccaga ctttatggat gctagaatca atgttctatg ggctagcgct ccaagcacaa
      481 tcttgccaga tacgacctac tatgcagaag aacatcctca gcgcgaagag ctcttgactc
      541 tttggaaaga aagcagcgca aatctcctca aggcttatga tttctctgat gaagaaattg
      601 aagacttgct agaaaaaaga cttgaattgg accgccgagt tgcggcagtg gtgctctcta
      661 atgaagaaag ttcagaatat gctaaactct atcatccata ttcttacgaa gatttcaaga
      721 aattcgcgcc tgccctacct ttggatgact tcttcaaagc agttattggg caattaccag
      781 acaaggttat tgtagacgag gaacgtttct ggcaagcagc agagcaattc tacagtgagg
      841 aagcctggtc tctccttaaa gcaaccttga ttttgagtgt tgtcaatctt tcaaccagct
      901 atttaacaga ggatatccgt gttttgtctg gtgcctacag ccgtgccctt tctggagttc
      961 cagaggcaaa agataaggtc aaagcagctt atcatctagc acaagaacct ttcaagcaag
     1021 ccctgggtct ttggtacgcc cgtgagaagt tctctccaga agccaaggcg gatgtggaga
     1081 aaaaagtggc aaccatgatt gatgtttata aggagcgtct gcttaagaat gactggctca
     1141 ctccagaaac ctgtaaacag gctatcgtga agctcaatgt gatcaaacct tatattggct
     1201 atccagaaga attgcctgca cgttacaagg ataaggtagt gaatgaaact gccagtcttt
     1261 ttgagaatgc tctagccttt gcgcgtgtgg aaatcaagca cagttggagt aagtggaacc
     1321 agcctgtaga ctataaggaa tggggcatgc ctgctcatat ggtcaatgcc tactacaatc
     1381 ctcagaagaa cctgattgtc tttccagcag ccattttaca ggcgcctttc tatgacttgc
     1441 atcagtcatc ttctgctaac tacggtggta ttggggcagt gattgcccat gaaatttccc
     1501 acgcctttga tactaacggg gcttcctttg acgaaaatgg tagcctcaag gattggtgga
     1561 cagagagcga ctatgctgcc ttcaaggaga aaacacaaaa agtcattgac caatttgatg
     1621 gacaggattc ttatggagca accattaacg gtaaattgac tgtatcagaa aacgtggctg
     1681 acttgggagg aatcgcagca gcgcttgaag cagctaagag agaagcagac ttctcagcag
     1741 aagagttctt ctacaacttc ggtcgcatct ggcgcatgaa aggtcgacca gaatttatga
     1801 aacttttggc tagcgtcgat gtgcacgcac cagccaaact ccgtgtcaat gtgcaagtac
     1861 caaacttcga cgatttcttt acaacctatg atgtcaaaga aggagacgga atgtggcgtt
     1921 caccagagga gcgcgtgatt atttggtaat acaaaaaatc tagtcatcag aaaaaaggca
     1981 tccaatcacg tgtgaaaacc ttgataggat gccttttgta atagaaagat ttgctggata
     2041 gtttacttaa atttcgattg tcatcatctt gctagaaaaa gggattgaag gccttttcgt
     2101 gagcgatagt agtagctgga ccatgtcctg gatagacatc gtagtttggt agggtgaaga
     2161 gttgggtctg gatactatga aggagttgct ccatgctacc agtcggaagg tcggtccgtc
     2221 cgatagtttc gcggaataga gcatctcccg tcaagactag gtgagcatca ggaaagacta
     2281 gggaaacacc accgatagag tgccctgggg tcggtagaac cttaaaacga aattcctcta
     2341 gttggtattc ttcgtgaaag acaaaggtgt gttctgcagg ttttgtgacc acatctgcca
     2401 tatcatcgtg gcgagggaga ccggagagat tatcgacagg agtgtagagc cagctggctt
     2461 cgctctctgc gatatagaca ggaggattgc caaacgtctc gcgaaccaag tccagactca
     2521 tgatatggtc ataatgggcg tgggtcaaga gaatagcaca gatcggtttg ttgatcttct
     2581 cgattgtctg acgaatggct tcccaatgac taccaggatc gacgacgatg aggtgctttt
     2641 cgccttctag ataataggta ttttcatagg caacaggatt cacggtttta tggattttca
     2701 tactagctcc aatctcaaag aatgtactaa tactcttcga aaatctcttc aaaccgcgtc
     2761 aactttatct gcaacctcaa agcagtgctt tgaacagcct gcggctagct ttctagtttg
     2821 ctctttgatt ttcattgagt ataaattaag tatagcacag ttagggagaa taggtaagga
     2881 tttaaaatga acttaaaaac cagcatgact agatgaagac aggctggtta tcaatttatc
     2941 aatattggaa aggtgtcaat tgcccttcat aggttgcata aactccgtca ttagcttgtt
     3001 tgatagacgt gatgtttaga gtgctaccgt agttggcttc tcatttgtcg ctatattcac
     3061 tataagttat cctactgggc aggttctggt cgttagcata gtattgaacg agttttttat
     3121 agctttgctg gctgaatagg aacaggcagc aaactcctaa cattaggaga gcaaagatag
     3181 aaatagcagt ttttttcata ggtattctcc ttataaacta aaggatagaa gaaagtatct
     3241 tgaggatttt tatactcttc aaaaatctct tcaaaccgcg tcaacgtcgc cttgccgtat
     3301 atatgtgact gactttgtca gtcttatcta caacctcaaa gcagtgcttt gagcaacctg
     3361 cggctagttt cctagtttgc tctttgattt tcattgagta ttaattccaa aatccagtca
     3421 aggtaccagt gtatgtgata cgagttccac cgtttatctt tcgtatcttc aatggaattg
     3481 gggaaatcag aaagtctttg ggttacaata tggtttccgt tcttctatct ctccttctat
     3541 cacctaagtt taaaatctaa cttcgtagta tattatgggt caataattca gaattatcaa
     3601 gcaattttga attatttttt tcttattctg ttccaatcta gttttcatat tcttaaaaaa
     3661 gtgataaaat ggtaggaaga attggagagt agagatgccg aaagaagtga atttaacagg
     3721 cgaagaagtt gtcgctttaa ccaaagaata tttaacggaa gaggatgttc attttgtcca
     3781 taaggccttg gtctatgctg ttgaatgcca cagtggtcaa tatcgcaaat caggcgagcc
     3841 ttatatcatt caccctatcc aagtggcagg tattttagct aagctaaagc tggatgctgt
     3901 aacagtagct tgtggattct tgcatgatgt ggtggaagat acagatgcga ccttggacga
     3961 tttggaaaga gagtttggtc ctgatgtgcg ggtgattgtt gacggagtta ccaagcttgg
     4021 caaggtcgag tacaaatcga tcgaggagca attagcggaa aatcatcgca agatgctcat
     4081 ggccatgtct gaggatatcc gcgttatttt ggtcaaactg tctgaccgct tgcacaatat
     4141 gcggaccctg aaacatcttc gaaaagacaa gcaggagcgt atttccaaag aaaccatgga
     4201 aatctatgcc ccacttgccc atcgtttggg gatttccagt gtcaaatggg aattagaaga
     4261 cttgtctttc cgttatctca atccaacgga gttttacaag attacccata tgatgaagga
     4321 aaagcgcagg gagcgtgagg ccttggtgga tgaggtagtc acaaaattag aggagtatac
     4381 gacagaacgt cacttgaaag ggaagattta tggtcgtccc aagcatattt actcaatttt
     4441 ccgcaaaatg caggacaaga gaaaacggtt tgaggaaatc tatgatctga ttgctattcg
     4501 ttgtatttta gatacccaaa gtgatgttta tgccatgctt ggttacgtgc atgaattttg
     4561 gaaaccgatg ccaggtcgct tcaaagacta tattgccaac cgcaaggcca atggttatca
     4621 gtctatccat acgactgttt atggaccaaa agggccgatt gaattccaga ttcgaaccaa
     4681 ggaaatgcac gaggtggctg agtatggggt tgcggctcac tgggcttata agaaaggtat
     4741 aaaggggcaa gttaacagca aggaatcagc tattggaatg aactggatca aggagatgat
     4801 ggagctccaa gaccaggctg atgatgctaa ggaatttgtg gactctgtta aggaaaacta
     4861 tctggctgag gagatttacg tttttacccc agatggagct gtccgttccc ttcccaaaga
     4921 ttcaggaccg attgattttg cctacgaaat ccataccaag gtcggtgaaa aagcaactgg
     4981 tgccaaggtc aatggccgca tggttccact gacaaccaag ttaaagacag gggatcaggt
     5041 tgaaattatc gccaacccga actcctttgg acctagccgt gactggctca atatggtcaa
     5101 gactagcaag gcgcgcaata agattcgcca gttctttaaa aaccaagata aggaattgtc
     5161 tgtcaacaag ggtcgtgaga tgctgatggc tcagttccaa gaaaatggct atgtggcaaa
     5221 taaatttatg gacaagcgcc acatggatca agttctgcaa aagaccagtt acaagacaga
     5281 agactccctc tttgcggcca ttggttttgg ggaaatcggt gcgattaccg tctttaaccg
     5341 tctgactgaa aaggaacgcc gtgaggaaga gcgtgccaag gccaaagctg aggcagagga
     5401 gcttgtcaaa ggtggcgagg tcaaggttga aaataaagaa accctcaagg tcaagcatga
     5461 ggggggagtg gttattgaag gtgcttctgg tctcctagtg cggattgcta agtgttgtaa
     5521 ccccgtgcct ggtgacgata ttgttggcta cattaccaag ggtcgtggtg tggctattca
     5581 ccgtgtggac tgtatgaacc tgcgtgccca agaaaactac gagcaacgtc tccttgatgt
     5641 ggaatgggaa gaccagtact ctagctcaaa taaggagtat atggcccata tcgatatcta
     5701 cggtctcaac cgtacaggac tgttgaacga tgtactgcaa gttctttcaa atacaaccaa
     5761 gaatatttca acggtcaatg cccaaccaac caaggatatg aagtttgcta atatccatgt
     5821 gtccttcggt attgccaacc tctctacact gaccacggtt gtcgataaaa ttaagagtgt
     5881 gccagaagtt tactctgtca aacggaccaa cggctagttg tcctagctct tactagaaag
     5941 gctattatga aaatcattat ccaacgggtt aaaaaagccc aagtgagtat agaaggccag
     6001 attcagggaa aaatcaatca gggactttta ttgctggttg gtgttggacc agaggaccaa
     6061 gaggaagatt tggactatgc tgtgagaaaa ctggtcaata tgcggatttt ttcagacgca
     6121 gaaggcaaga tgaacctgtc tgtcaaagat attgaaggag aaatcctctc tatttctcag
     6181 tttaccctct ttgcggatac taagaaaggc aatcgtccag cctttacagg ggcagctaaa
     6241 cctgatatgg catcagactt ctatgatgct ttcaatcaaa aattagcgca agaagtgccc
     6301 gttcagacag gcatctttgg agcagatatg caggttgagc tggttaataa cggacctgtt
     6361 accattatcc tagatactaa aaagagatga gaaagaccaa gcccagtcgg cttggtcttt
     6421 ctcatctatc ataaaatact ccaaaaagaa atcggttctt gatatgcttg ggggaagtct
     6481 cttttcaggc tttggcagat gcgataggaa gggatgagat gtcctaggat gaggagagtt
     6541 ccctgaagga aaagtggaat atcacttaaa gacaccaagg agagaatcat caggctatcc
     6601 cataggagaa tttgtaaagc aaaacgccag aatctcggtc taatctggga atagagttaa
     6661 ctaaagtggt cacaaagttg gtttgaaaga taggtcaata gcacaggatg aaagaaaatg
     6721 agccaaccgc tataaatcaa aatccagtcc caagtaagct cccttttaaa tgtcaaaaat
     6781 gccagcatga ttccatcaat gacaaggagt tgaatggttt tgatgaagat gatttttttc
     6841 atgctaaaac ctccttttcc ttagagacac tcccataaag agatgtgttt gtcgtaaaag
     6901 tctggatagt tttctcctag gtagtcagct gtcatataat aaaaagtaga atggcgagca
     6961 gtgatgattg gcataagaga tgaaaaacgc tgagaactaa tgaaggctaa gagaacaaaa
     7021 aatagaaaat caatggtgag aatccctaaa tagtagaagc gaatcttttt attgatttga
     7081 ggataaatct cagcgaattg atgtttgagt cggacgacca agagaaatag caggagtcct
     7141 tgagcaagga agaaaattaa accagagagc aagagccact caaaagatgt ctcgagtcta
     7201 gtgataaaaa atgcaaatag tagcaaatca ataactgcaa ttgctataaa atggattcta
     7261 tagagttttt tcatgacaag acctccctct tttatctaac ttcattctac tctaaaagaa
     7321 tgggagttac aactaaaatg ataaaaatag cagaggggag attctcttaa gttgactagt
     7381 attctttatt tgagtttcct tctattatct aacttcttca tcattccaga caaataaagc
     7441 tccgattgca ttgaggatat aaaagatgta tttaccgata ttggcgaagt ttccttgaat
     7501 accagctttt gtcagctgaa cgaaattgta aatcaaccaa aagccccact gtccctcatt
     7561 gcaaatgcat tcaaagcatt ggcaatgagg gacagtgcaa aggcaatagt tgttacgtag
     7621 gcaaggagat tcatcttgcc cccatatccg atatagttgg tcacaaaggc aaagaggaag
     7681 gcgatgatgg aaatgatgat ggccgccaat tttacctgtt tttggctcat ttggttgggt
     7741 ctgccttctt gcgaagcttt ccatttcttt atagcaaagg tataaatgag gaaggtgacg
     7801 ggataggtaa tgatggccgc cttatttcca aggatataat caatagcacc ggacaaaatg
     7861 gtattaacaa taccaaagta atttccccat ttgcttaatt tccccgtgaa acgagtggac
     7921 aacatggaaa tcccaacgtt ggttacggaa atcaatccaa agggtacaag agctgtccat
     7981 agtccccagt ctacaaattt atcgaggcgt gagttgagat aaccagatgc aatcgcaatc
     8041 ccaacgacca aagcaacccc gaagaggtca aatcatttag atgtagcaaa aatttttagt
     8101 gattttttca taggttaaac tacctttctt tttttcaaat actctaccac caaatgaaag
     8161 taaaatcaaa tgatagaaat aaaaccctga aaataaaact ttcagggttg gtagggaact
     8221 tgagaaatta gttgattttc tcgacataga gttgttttgc aatgtccata ttcacttgta
     8281 aatcctcgtc gttactgagg atagtgaagg tattgctgaa gccatcaaac tgcttgactt
     8341 ggagctggtc accgatgtga agtgagtgct tgtccagata atggagaatg tcaaaactat
     8401 cgtgcacccg agtcaggcgg taggcgccag cttccttgat atcagctagt gggaggttat
     8461 tgatttcaac gaggagttct cccttggcag gaatagttcc tccgtggggg caggttttag
     8521 ggaaacctag cagtttatct agtctttcca cgaacaggtc agagacagtg tgttccaaga
     8581 cctcagcttc ctcgtgaatc tggtcacttg tatagtctaa atgatgaact agaaaaactt
     8641 caatcaagcg gtgcttacga tagagctcag agaccagttt gagaccgagg tcagtcagta
     8701 gatagccaca ttccttgtcc tttaggatga gattttcact tttcattcgt ttgatcattt
     8761 cagttacggc agggggagag acttgcatgc gagccgcaat ttccttgttg gtaatcttat
     8821 gcaggtctat gccaatttca taaatacatt ttagataatc ttctttgttt ggggtcattc
     8881 gtttcctctt gtctaatatc tttatctagt atatcaaaaa aagctagatt tctctagctg
     8941 tgacttttta tgttatactt attgcaagag aaaaaacgag gagatggata tgacgaaaat
     9001 agctcttctt tcagatattc atggaaatac caccgccttg gaggctgttt tggcagatgc
     9061 tcagcagcta ggagtggatg aatactggct tttgggagat attctcatgc cagggacagg
     9121 acgtagaagg attttggact tgttggatca actaccgatt acggctagag ttttgggaaa
     9181 ctgggaagac agtctttggc atggtgtccg taaggaattg gacagtactc gccccagtca
     9241 acgctatctc ttgcgccagt gccagtatgt tttagaggaa atttccctag aagaaattga
     9301 agtgctccac aatcaacctc ttcaaattca tcgtcagttt ggggatttga cggtgggaat
     9361 tagccatcat ctgcctgata agaactgggg gcgagagttg attcatactg gcaaacaaga
     9421 ggagtttgac cgcttggtga ctcatcctcc ctgtgatatt gctgtttatg gacatattca
     9481 ccagcagttg cttcgttacg ggactggtgg gcaattgatt gtcaatccgg gttcgattgg
     9541 ccaacctttc tttttggacg ctcagctacg gaaggacttg cgggcccagt atatgatttt
     9601 ggagtttgat gacaaaggcc tggtagatat ggacttccga cgggtagact acgatgtggc
     9661 agcggaattg cagctggcta aagacctgag acttccctat tttgaggttt actatgaaag
     9721 tctggtcaat gggatccacc atactcatca tcaggaattt ttgagagaat tggctcagaa
     9781 agagggctgc gatcgggagt tagacgactg gttgaaaagt ggtaacgatt gacattacaa
     9841 ctaaaaaggg tataataata gaaaaagaag agtagaggca gggtagcctc gtaaaaagga
     9901 gaagaggatg caaattccaa gtagatttac cattgcaact catatgctga taatcattgc
     9961 cctcgagggg aaggaaagca aggtgaccag tgattttctg gctgctagtg ttggggtcaa
    10021 tcctgtcatt atcagaaaga tcttgtccca gttgaagaag gcagagctga tttcagtagc
    10081 gcgtggaacg ggcggaacag agattgtcaa ggaccttaag gatattagtc ttttagatgt
    10141 ttatcaggcg gtcgaatgtc ttggtaagac aggtcaactc ttcagtttcc atgacaatcc
    10201 gaatccaaat tgccctgtag gagctcatat tcatgatgtt ttggatcaaa aattggagag
    10261 aattcagttg actatggagg cagaacttgg tcagaccagt ctagaaaaag tcgtggccga
    10321 tgcagagagt cagatgaagg attaagaggg ataaatgccc tctttttttc tttggataat
    10381 tatgataaaa tgtagctatt aaaggagtaa gaaatgtatc tcgttataga aattgtatta
    10441 gcctttatag tgtcattttg gaaggataat agaagtttgt ggaatccagt attattttta
    10501 ttatccctca tttcaagtta tttttatcta tcctaccttt tttataaaaa tggatacgag
    10561 aatgttcagt tagcttttta tgtatttgcc tttgtcttac ttcctttttt gctttttcta
    10621 agtggcatct ttttaatcta tagtggggtc attttgttga aacgagaggg acgttccaag
    10681 ccccattatc tctcaatgct ctttgatttt tattgactat tagattctat ttaaaaagac
    10741 ttagaatgca catgaaagta tgtattctag gtcttttgtt ttgtatacat ttaattttca
    10801 agagtattat ctatttttcc atgcatggta acgagtatca atcaataact gggtaaggcg
    10861 acccaaggtt tctctttctt ctggagtgag ggattgagct tggacaaaga gatgatgaat
    10921 gtattcaagc gcctttaagg aagccaagtc attgatagag ctaataccag catcaagaat
    10981 agtgccaaag aagaggtcga agtagagctg aagttcttcg tcagggactg tggccaccca
    11041 ttctttaaag gttgtgtcta cttgccggct atcactgttg gtcttatcca gttgaacgaa
    11101 gtgcttgtcc tcaatcttcc aattaaaggt atcgtgctgg gcgatgccac ccagggcagt
    11161 actctgaacg atgatttggt gagcaggaat ttccagcatc ataccgataa tggaaccttg
    11221 tggaatgaat atcttgcttc tatccattat cctttgataa cccgcagtct gtgtcaattc
    11281 ttgatggaga ccaggtgcat caaatgtata aactgctgtg atctgatttt gcaaactttg
    11341 ctcaatttgg ctagcagcat agatagcgag atttcctccc ttggaatgcc cagccagaat
    11401 aaccttttgc ttaggatgat gggcaaaaaa gttctttaaa tagcgaaggg cgtgcttttg
    11461 agcaggaatt tccttcatat aggtcaggtg gaaatcttcc ttccagccaa tgatactgtc
    11521 atctgtccca cgaaagacaa tcagataggt atcgaggctg acacgataag tcatagccgc
    11581 aaattgcttt tgcagttcag ggtcgatgtc gttgataaaa tgggagagtt tgcaattttt
    11641 gaagcgcttg tgttgagcca attcatctaa taattgaagg cgatttttgc tagtaagcat
    11701 ggtgggctct cttggaacct gaggtgctag atctaaaaga cgttgaggaa gtgtggagac
    11761 cagattatca aaggagaggt aggtgatttc tattaaggtt agaatgtcta actcattcaa
    11821 gggaaggtcg taataagaat catatgcgac atctttcaga tagtcaaaaa tattggccat
    11881 aagagctcct ttctttttat ttatcttagc aaatttccct caaattagct agtagcattg
    11941 cctgtttgta ctggctaaaa acaggctatt tcaaattcag tttcagacca tctggcatgg
    12001 aaaaatctgt tataataatg gaaaaggaga agcgcatgca caagatttta ttaatagaag
    12061 atgatcaggt cattcgtcaa cagattggga aaatgctctc tgaatgggga tttgaagtgg
    12121 tcctggtaga agactttatg gaagttttga gtctatttgt tcagtcggaa cctcatctgg
    12181 tcctcatgga tattggtttg cccttgttta atggttatca ctggtgtcag gaaatccgca
    12241 agatttccaa ggtacctatc atgtttcttt cttcgagaga ccaggctatg gatattgtca
    12301 tggcaatcaa tatgggggcg gatgactttg tgaccaagcc ttttgaccag caggttcttt
    12361 tagctaaggt tcagggcttg ttgcgtcgtt cctatgagtt tgggcgtgat gagagtttgc
    12421 tggaatatgc tggtgttatc ctcaatacca aatccatgga tttacattat caagggcaag
    12481 tcttgaattt gaccaagaat gaattccaga ttttacgcgt gttatttgag catgcaggca
    12541 acatcgtagc acgtgacgac ctgatgcggg aactttggaa cagtgacttt ttcattgatg
    12601 ataataccct ctctgtcaat gtggctcgtt tgcgtaaaaa gttggaggag cagggattgg
    12661 taggatttat cgagaccaag aaaggaatag ggtacggatt gaagcatgct tgattggaaa
    12721 caattttttc tagcctatct gcgctcccgt agtcgtcttt ttatctatct gctttctttg
    12781 gcatttcttg tcttactctt tcagttttta tttgccagtc taggaattta cttcctctac
    12841 tttttcttct tgtgttgctt tgtaaccatc ttatttttca cttgggacat attggtggaa
    12901 acgcaggtct atcgccagga acttctctat ggagagaggg aagccaagtc tcctttggaa
    12961 atagctttag cagaaaaatt agaagcgcgt gagatggaac tctatcagca gaggtcaaaa
    13021 gcagaaagaa aactgacgga tttgctggat tactatacct tgtgggtcca tcagataaag
    13081 acccccattg cagccagtca actcttagtt gcagaagtgg tcgaccgcca actgaagcag
    13141 cagctagaac aggaaatttt caaaatcgac tcctatacca acctagtttt acagtacctg
    13201 cgtttagaaa gtttccatga tgatttgctc ttaaagcagg ttcaaattga ggacttggtc
    13261 aaggaaataa ttcgtaaata tgctcttttc tttattcaaa aaggcttaaa tgtcaatcta
    13321 catgaccttg ataaagaaat cgtgacggat aaaaaatggc tgctagtggt tattgagcaa
    13381 atcatctcaa acagtctcaa gtacaccaag gaaggtggtc tggagattta tatggatgac
    13441 caagagcttt gtatcaaaga tacgggaatc gggataaaaa acagtgatgt cctccgagta
    13501 tttgaacgtg gcttttcagg atacaatggc cgtttgaccc agcagtcctc tggacttggc
    13561 ctttatctat ctaagaaaat ttctgaagaa ctggggcacc agattcgtat cgagtctgag
    13621 gtcggaaaag gaacgacagt gcggattcag tttgctcaag tgaacttagt ccttgagtaa
    13681 aaatagatga gaaaaagcct ccagattgga ggcttttatg ctagactctt atttctcaac
    13741 gcgtgatttg ttggcgatat cagctaggat agcttgaaca aaattatcaa ctgagacagt
    13801 ttgtgtttct ttttggccgt agcgacgaac gttgactgtt tcgtcttcca tttctttgtc
    13861 tccaacaatt aattggtaag gaatcttgct ggtttgtgaa gcacggatct tgaactgcat
    13921 tttttcattg cgctcatcta cgtctgcacg gacaccgcgg tcacggagtt tcttggccac
    13981 ttcccaagcg tagtccacgt gtttttcgtt agatactggg atgagggtta cttggtgtgg
    14041 tgccagccat gttgggaagg cccccttgta gttctcaatc aagatagctg tgaagcgttc
    14101 catagttgag ataaccccac ggtggatcat gactggacgg tgatcttcgc catcagctcc
    14161 gatgtatttg aggtcgaagc gttctggcaa caagaaatca agttggatag tagaaagggt
    14221 ttcttctttt ccaagggcag ttttaatctg gatatccaat tttggtccgt agaaggctgc
    14281 ttcaccttcg gcttcaaagt agtccacgcc catttcatca agagctgcac gaagcatggt
    14341 ttgggcattt tcccacatct catcgttatc aaagtactta tgagtatctt gagggtcacg
    14401 aagagagagg cggaagcggt agtcagtcaa gttgaagtct tcataaacat cgataatcaa
    14461 ctgaagggca cgttggaatt cttcttggat ttgttctgga gtaacgaata ggtgaccgtc
    14521 gttgagtgac atttcacgta cacgttgaag gccagtgagg gcaccagatt tttcgtaacg
    14581 gtgcatcata ccgatttcag cgatacggat tggcaattca cggtaagagt gaacatggtg
    14641 tttgaaaact tggatgtggt gcggacagtt cattggacga aggacaaatt cttccccgtc
    14701 acccatgtcc atggttggga acatgtcttc ttggtaatga tcccagtgac cagaagtctt
    14761 gtaaagctca acagaagcaa gtggtggagt gtagacgtgt tggtagccag aagccaactc
    14821 tttgtttacg atgtagcgtt ccaattcacg acggatagtc gcaccatttg gcaaccagaa
    14881 cggcaaacct tgtcccactt cttgtgaaat cataaagagg tcaagctctt taccaagttt
    14941 acggtggtca cgttccttag cttcttcacg catttgaagg tagtttttca agtctttctt
    15001 gtcaaaccaa gctgtaccgt agatacgttg catcatagcg ttgtcgctgt ttccacgcca
    15061 gtacgcacca gctacatgga gaaggtggaa gatttggata cgacctgttg atggaacgtg
    15121 aggtccacgg cagaggtcta catattcacc ctgacgatag atagtcaaac cgccttcgtc
    15181 ttctgagtgt tcttcaatca attccaactt gtaagggtca tttttgaaga tttcacgtgc
    15241 ctcgtcttta gtcacttctt cacgaataga tgggaagttt tctttgacga ttttttgcat
    15301 ttcttcttcg atacgaggaa ggtcttcgtt agagatttga ccagctgtgt tgtcagtatc
    15361 gtagtagaaa ccatcttcga tggctggacc aactcccaag tgaatgtccg ggaaaagacg
    15421 acgagctgct tgggcgaaca agtgagctgc tgagtgacgc aagattggaa gggcatcttc
    15481 gtgatcaggt gtcacaattt cgatgcttcc atcttcagtg atagcgcgag tagtgtcgat
    15541 gagtttgccg ttgaatttac cagccaaggc ttttttagct agggaattgc tgatagattg
    15601 ggcaatttca aaagttgtta cgccagattc gaattcacga acagcgccat ctgggaaagt
    15661 aatgttaatc atgatgttct ccttttttat tatttcatag tttctaagca ttgatttttt
    15721 atcagcagac tacttccgag tcctaacgtt caattgcttt tctttatcag agaggaagtt
    15781 ttccatttcc gtcagaatgt ctatatccaa acgttgagaa agttctaata gccaccagat
    15841 attttctgaa agtttttgtt ccagtgtgta gggtgtttca tcatagtagc gtccttgctt
    15901 tgtcatcacc agtcattgga aatttccaat atcattagat aaagccaaga ggtcttcttc
    15961 taccgtccac ttgaaatcat gatgcttaac ttccagttcg tgataagctt gtcggattgc
    16021 ccaactgcgc tccactaatt cttgcaactc cataattagt atcctaataa aaaaatgcga
    16081 catcctcgaa aggacgtcgc aacgtggttc caccttcatt tatgtacctc aaaagagagg
    16141 gacacctctg atcggctcta acgtggccac cgttttatgt tttcataaaa aactcaagag
    16201 tagtatcagt ttagacttcc tatgcgtttc cagcaaccac gcactctcta gtagaaaggg
    16261 actaagtgac ttgtctctat ggattattat agcatgattg tgagattttt caaggatttt
    16321 gtgaaagttt tttgtttttc attttttgta gaatatgctt gatgctaata ccagaatcca
    16381 tgctcaccag agcaactgtt tcatagatta gcaagtaaat tagtataaac tcaccaaagg
    16441 ttatgatagt aaagtaaaag agcagtccgt taccaactaa ccaccaaagt gagaaacgga
    16501 aacggtagct atagagtaac gatataatca agattaccag aggtgtaaag aagagaagat
    16561 tgtttatata gagtcccttg agtgtaaggg ggtcagaaac aactagcaag aacaactcat
    16621 aaagtggcca ataaaagaat aatatgacca gatagtaggg gagatgggaa tagaatctac
    16681 gcatgataat cacctcgttt taaaaaaaca gacaacttag atctcttgct atgattatta
    16741 tagcatgttt aaaacaagaa agtaaatttg ttgacaaaga agatttttct tttccctaga
    16801 atcatagtta gttttcttgg tagaaatatt ttgataataa ttaagcaaga ctattgacat
    16861 tgattattaa gagtgtcaca attgttgtaa ctaaaataat tacaacaaga gaggtataaa
    16921 aaatgaaact agcagttatt gcagcaaatg gacaagcggg gaaggcaatt gttgaagaag
    16981 cagtcaaacg aggacatgaa gtaacggcta ttgttcgttc tgaaaacaaa agtcaatctg
    17041 agtctattat taaaaaagat ttatttgagt tgacaaaaga tgatcttact ggatttgatg
    17101 ctgttatttc agcgtttggt gcttatacac cagatacgct accgctccac agcaaatcaa
    17161 tcgaactatt taatcagctt cttgctggaa cacagacacg ttttctagtt gtagggggat
    17221 caggaagctt ttatatagat gaaacaaaaa cgacacgact tttagatacg tctgattttc
    17281 ctgaagaatt taagccacta gcaaaagctc aagcggatga attggatctt cttcgtacaa
    17341 aaaataatct caactggact tttgtcagtc ctgctgtaga ttttattccc gatggtgaaa
    17401 aaacaggaaa ctacattcta gcaggagaaa tttttactac taatgaaaaa ggaataagcc
    17461 agattagtta tgcagattat gctattggat tggtagatga acttgaaaaa ggtcatcata
    17521 taaaagaacg tatctcttta cttgaaaaat aataactatg agagtattaa tgtttgacag
    17581 aaaatttaaa tcttggtaga atagggattg tcgtaaagac aaataacttc ttcttggtta
    17641 caggcatgcc aacctgtcac tcggatgaag ccaaataaaa aggagaaaca tcatggcaat
    17701 ctcaaaagag aaaaaaaatg aaatcatcgc acaatatgca cgtcacgaag gtgatacagg
    17761 ttcagtagag gttcaagttg ctgtccttac ttgggaaatc aaccacctta atgaacacat
    17821 caaacaacac aaaaaagacc acgctactta ccgtggattg atgaaaaaaa tcggtcgccg
    17881 tcgtaacttg cttgcatact tgcgtaaaaa cgacgttaac cgttaccgtg agttgatcaa
    17941 ctctctagga cttcgtcgct aattcaagat acaaaggctg tcaaaagcac aaagtaaaaa
    18001 taggaaaatt gacgaagaaa cttcagtttc taggagattt tatctttttt gccaagtgct
    18061 taggccgtgt tcaattgagc atatcttgat aatgaagcta ctctaaattg ggtagctttt
    18121 ttgtgttgtc accttacctc gatatactca agtataatct ttggttacgg ttcctagcac
    18181 tgtaaggtaa aataaaccag cttttctccc ttcggggaga ttttttttgt ttcactgaaa
    18241 gtttcgtacg atcttcgcct cgtcgcctag tctataaacg atttatctag caagaatcat
    18301 gatgctaagg gcgtcaaaaa tccgtatgaa aatagggaaa tgacacagtg ttcgatgagc
    18361 acaaggagtt tcatcttttt cactaggatt ttagcccgga ctcaaatcag ctctctgact
    18421 tcagagggct tttttattga ggttttatcg gtaacaattt tggagactac aaaaacctca
    18481 aacggtatca gctaattaca ccataaaggt gcgcagttac tcggcttttc aagccgagta
    18541 gttgtatgca agccccctag aagagcccac tctttacaaa gtaaagtata gtatgttata
    18601 ctttacatgg aagtagtcac tgaattccag ttagaaatta ctttgtaact acgttttgag
    18661 gaggagtaaa atgctttcct acgttcgata ttacccacta gcgatagcta aattaatgtg
    18721 tctgtgctct cctaaaatct gctgatttat tactgactaa tacaggagtt ttttttatgg
    18781 gacagacaat catatctgct attggtgttt atatttccac cagtatcgat tatttaatta
    18841 ttttaattat tttatttgca cagctatcac agaataaaca gaaatggcat atttatgcgg
    18901 ggcaatatct aggcacaggc ttacttgtag gggcgagttt agttgctgct tatgtcgtta
    18961 atttcgtgcc tgaagaatgg atggttggat tgcttggttt aatccctatc tatttaggga
    19021 ttcgctttgc aattcttgga gaagatgcgg aagaagaaga ggaagaaatt attgaaagat
    19081 tagaacaaag caaggcaaat caactgtttt ggacagttac attgctgaca attgcgtctg
    19141 gcggagataa tttaggtatc tatatacctt attttgcttc gttagattgg tcacagaccc
    19201 tcgtggcctt gcttgtgttt gtaatcggca taattatctt ttgcgagatt agtcgggtgt
    19261 tatcctctat tccgttaata ttcgagacaa ttgaaaaata cgagcgaatc attgtgccct
    19321 tagtattcat tctacttgga ctatacatca tgtatgaaaa tggcacgata gagacttttc
    19381 tgatcgtgta gatttttttg tttcactagg attttagccc gagctcaaat cagctctctg
    19441 attttcagag ggctttttca ttgagtataa atggacaatc ttaaagttac tctattgtag
    19501 agtggctttt gatttgatta ggagattctt ataaatatag taaaatgaaa caaaaacagg
    19561 acaaatcgat caggacagtc aaatcgattt ctaaaaatgt tttaagaagt agaggtgtac
    19621 tattctagtt tcaatttgat atataaatga aaaccttagc tgtttggcca aggttcattt
    19681 cttctatgca agttcttctc ttttcttatc tgggttccag atgaagcttg cgataaagct
    19741 aaagatgatg gttaggatag cgaggataat agcaaggatg agggcaggga tgcggataaa
    19801 ccaccagagt ggatttggtt ttttatcatt gtgccattgt ttcgtttctt cgtccagacg
    19861 ttggaattct gtttgaatac gattggcgac tgtttcaatg ccttcatcat tcattttctt
    19921 gatatctgag atatcgattg gatttccaaa gttcatatcg acacgttcac ggctaatcaa
    19981 gcccttcaaa gtcatgggac cggtgtaggt aaccggcatg atacggactt tggccatttt
    20041 ggcaatcagt gctgcgcccc ccttgacatc gtttgagtgg cggctaccac ttggaaacat
    20101 gatgagagag cggtcacttt ttttgagaac gttgatagga tatttgatgg ctgaggcgct
    20161 gggattttca cggtcgatgg gaaaggcgcc acacatacga atccaccaac caaagatacg
    20221 gttggtaaag agttcttttt ttgccataaa gatgaactgt tttggcttgg tcgcaaaggc
    20281 catataaaca ggatcccacc aggtacggtg aggcgcaact aaaatataat tttcatcttg
    20341 attaggaatt ttatcagtat tatgatagtg agcattgcca ttgatggacc ataagagcaa
    20401 tacaactaat ccacgcaaat aagtataaaa catgcgatct ccttcgattg ttttcttgtt
    20461 attattatac cttatcaaag gagggctggc aaacttttcc cttgactaga tgcatatttg
    20521 ggatgaaatt agaattttgt taaaaaaaat gatataatag aatttatgga taaaaataag
    20581 attatgggat taacccaaag agaagtcaag gaaagacagg ctgagggttt ggtcaatgac
    20641 tttaccgcat cagccagtac cagcacttgg caaatcgtta aacgaaatgt ctttaccctt
    20701 tttaacgctt tgaacttagc cattgctttg gctcttgcct ttgtgcaggc ttggagcaat
    20761 ctggtcttct ttgctgttat ctgctttaac gctttttctg ggattgtgac cgagctacga
    20821 gccaaacaca tggtggacaa gctcaatctc atgaccaagg aaaaggtcaa aaccatccgt
    20881 gatggtcagg aagttgctct taatcctgaa gaattagtgc taggagatgt cattcgtttg
    20941 tctgcaggag agcagattcc tagtgatgcc ttggttttgg aaggctttgc ggaagtcaat
    21001 gaagccatgt taacgggaga aagtgatttg gtgcaaaagg aagttgacgg cttacttttg
    21061 tcaggaagtt tcctagccag tgggtcagtt ttatctcaag ttcaccatgt cggtgcagac
    21121 aactatgctg ccaaactcat gcttgaggct aagaccgtta aacccatcaa ctcccgtatc
    21181 atgaaatcgc tggacaagtt ggctggtttt actgggaaga ttatcattcc ctttggtctg
    21241 gctctcttgc tggaagcctt gcttttaaaa ggcctgcctc tcaagtcatc tgttgtaaac
    21301 tcgtcgacag ctcttttggg aatgttgcct aagggaattg cccttttgac cattacttcg
    21361 ctcttgactg cagtgattaa gttgggcttg aaaaaggtct tggtgcagga gatgtactct
    21421 gttgagacct tggcgcgcgt ggatatgctc tgtctggaca agacgggcac catcacccaa
    21481 ggaaagatgc aggtggaggc tgttcttcca ctgacggaaa cttacggtga agaggctatt
    21541 gccagcatct tgactagcta catggcccat agtgaggata agaatccaac tgcccaagcc
    21601 attcgccagc gttttgtggg agatgttgct tatcctatga tttccaatct tcccttctcc
    21661 agcgaccgca agtggggggc tatggagtta gaaggcttgg ggacagtttt cttaggggca
    21721 cctgagatgt tgctggattc tgaagtccca gaagccaggg aggccttgga gagaggatca
    21781 cgtgtcttgg tcttagctct cagtcaggag aaattagacc atcacaaacc acagaaacca
    21841 tctgatattc aggctctagc cttgctggaa atcttggacc ccattcgaga gggagcagca
    21901 gagacgctgg actatctccg ttctcaggag gtgggactca agattatctc tggtgacaat
    21961 ccagttacgg tgtccagcat tgcccagaag gctggttttg cggactatca cagctatgta
    22021 gattgctcaa aaatcaccga tgaggaattg atggccatgg cggaggagac agctattttc
    22081 ggacgtgttt cccctcatca aaagaaactc atcatccaaa cgttgaaaaa agcgggtcat
    22141 acaacggcta tgacagggga cggggttaat gatatcttgg cccttcgtga ggcggattgt
    22201 tctatcgtga tggcggaggg ggatccagca acccgtcaga ttgccaatct ggttctcttg
    22261 aactcagact ttaatgatgt tcctgagatt ctcttcgagg gtcgtcgcgt ggtcaataac
    22321 attgcccaca tcgccccgat tttcttgata aagaccatct attccttcct gttagcagtc
    22381 atctgtattg ccagtgcttt actaggtcgg tctgaatgga tcttgatttt ccccttcatt
    22441 ccaatccaga ttaccatgat tgaccagttt gtggaaggtt tcccaccatt cgttctgact
    22501 tttgagcgaa atatcaaacc tgttgagcag aatttcctca gaaaatccat gcttcgtgcc
    22561 ctaccaagcg ctctcatggt cgtctttagc gtcctgtttg tgaaaatatt tgggagtagc
    22621 caaggttggt ctgagttaga aatctcaact ctcctctatt atctcttggg gtcaattggt
    22681 ttcttatccg tatttagagc ctgcatgcca tttaccctat ggcgtgtcct cttgattgtt
    22741 tggtcagtag gaggtttcct agccacagct ctcttcccaa gaatccaaaa actgcttgaa
    22801 atttcaacct tgacagaaca aacgttgcct gtttatggtg tcatgatgtt ggtctttaca
    22861 gtgattttca tcctgactag tcgctatcaa gctagaaaat aaagaaagac tgcaatctgt
    22921 ggattgcagt ttttttaggt acaagattgc cagccgaaat gtggtataat aagaggtaat
    22981 agagttttgg aaagtgagag aagatgattt caaagagatt agaattggta gcttcctttg
    23041 tgtcacaggg ggctatttta ctagatgtgg gaagtgacca tgcttatctg cctatcgagt
    23101 tggttgagag aggccaaatc aaaagcgcta ttgcaggtga ggtggtggaa ggtccctatc
    23161 agtctgcggt taaaaatgtt gaggctcacg gcctaaagga gaaaatccaa gtccgtttag
    23221 ccaatggctt ggcagctttt gaagagactg accaagtgtc tgtcattacc attgctggca
    23281 tgggtggtcg tttgattgct aggattttag aagaaggttt ggggaagtta gctaatgtag
    23341 agcgtttgat cctccagccc aataatcgtg aagacgactt gcgtatctgg ctacaggatc
    23401 atggattcca gattgtagca gaaagcatct tagaagaagc tggaaagttt tatgagattt
    23461 tggtggtgga agcaggacaa atgaagctat cagccagtga tgttcgcttt ggtcccttct
    23521 tgtccaaaga agtcagtcca gtatttgtcc aaaaatggca aaaagaagct gagaagctag
    23581 agttcgccct cggacaaatc ccagaaaaaa atctggaaga acgtcaagtt ctagtagata
    23641 agattcaagc tatcaaggag gtgctccatg ttagcaagtg aagtgatcca agcttatgaa
    23701 gccttttgcc ctcaggaatt ttctatggag ggagacagcc gtggtctgca aattggcact
    23761 ttagacaagg gtatccaaag ggtcatggtt gccctcgata ttcgtgaaga gacagtggca
    23821 gaggccattg aaaagggtgt ggacttgatt atcgtcaagc acgcgcctat ctttcgtcct
    23881 atcaaggact tgcttgccag ccgtccgcaa aatcagattt acatcgacct gattaagcat
    23941 gatatcgcag tttatgtcag ccataccaat attgatatcg ttgaaaatgg gctcaatgac
    24001 tggttttgtc agatgctagg aatcgaggag acgacttatc tgcaggaaac aggtccagaa
    24061 cgtggaattg gacgtattgg gaatattcag cctcagacat tttgggaatt ggcccaacag
    24121 gtcaagcaag tctttgacct agatagcctt cgaatggtgc attatcaaga ggatgatttg
    24181 cagaagccta tttcaagagt agcaatttgt ggtggaagcg ggcagtcttt ctataaggat
    24241 gctttggcaa agggggcaga tgtctatatc actggtgata tctactacca tactgctcag
    24301 gatatgctgt ctgatggctt gttagcattg gatccaggtc actatatcga agtgcttttt
    24361 gtggaaaaaa tcgcaacact ccttactcaa tggaaagaga aaaaaggctg ggaactagag
    24421 attttaccta gtcaagcatc gaccaatcct ttccgtcata tctagttaga aagtgaagac
    24481 aatgaaaaaa gttgccatta ttggagcagg aattgtggga gcaacagctg cctactacct
    24541 ctcgagagaa agtgacctag aggtgaccgt ttttgaccat gagcaaggtc aagccaccaa
    24601 ggccgcagca ggaattatca gtccttggtt ttccaaacgc cgtaataaag cctggtacaa
    24661 gatggcgcgc ttgggggctg atttttatgt ggatttatta gctgatttag agaaatcagg
    24721 acaagaaatc gacttttacc agcgttcggg agtctttctc ttgaaaaagg atgaatccaa
    24781 tttggaagaa ctttatcaac tggccctcca gcgcagagaa gaatctccct tgatagggca
    24841 attagccatt ctgaaccaag cctcagctaa tgaattattc cctggtttgc agggatttga
    24901 ccgcctgctc tatgcttctg gtggagcgag agtagatggc caacttttag tgactcgttt
    24961 gctggaagtc agtcatgtca agctggtcaa agaaaaagtg actctgacac cgttagcatc
    25021 aggctaccag attggtgaag aggagtttga gcaggttatt ttggcgacgg gagcttggtt
    25081 gggggacatg ttagagcctt taggttatga agtggatgtt cgtcctcaaa aaggacaact
    25141 acgagattat cagcttgccc aagacatgga agattaccct gttgtcatgc cagaagggga
    25201 gtgggatttg attccctttg caggtgggaa attatcctta ggcgctaccc acgaaaatga
    25261 catgggattt gatttgacgg tagatgaaac cttgctccaa caaatggagg aggccacctt
    25321 gactcactat ctgattttgg ctgaagctac ttcaaaatct gagcgtgttg gaatccgtgc
    25381 ctacaccagt gatttctctc ctttctttgg gcaggtgcct gacttaactg gtgtctatgc
    25441 agccagtgga ctaggttcat caggcctcac aactggtcct atcattggtt accatctagc
    25501 ccaactgatc caagacaagg agttgacctt ggaccctcta aattacccaa ttgaaaacta
    25561 tgtcaaacga gtaaaaagcg aataatactc aataaaaatc aaagaacaaa ctaggaaact
    25621 agccgcgggt tactcaaagc actgctttga ggttgtggat agaactgatg aagtcagtaa
    25681 ccatacccat ggcaaggcga cgttgacatg gtttgaattg gattttcgaa gagtataagc
    25741 attttactga aattttatca ggtagtttag gatggcaaat gacattccct gtcaaaaatg
    25801 gtaaaataag aaaaaataat ctgagaatcg aggaaaaaag atgcaagaaa agattttggt
    25861 aactggtggt gccggtttta ttggaaccca cactgttatt gaattgatcc aagcaggcca
    25921 tcaagttgtt gtggtggata atcttgtcaa tagcaatcgt aagagtttag aagttgttga
    25981 aagaatcaca ggagttgaga ttcctttcta tgaggcagat attcgtgaca cagataccct
    26041 cagagatatt ttcaagcagg aagaaccgac tggtatcatt cactttgctg gtttgaaggc
    26101 tgttggcgaa tcaacacgta tccctcttgc ctactatgac aacaatatcg ctggaactgt
    26161 cagccttttg aaagccatgg aagaaaacaa ctgtaaaaac atcatcttca gttcttctgc
    26221 gacagtttac ggggatccgc acacagtgcc catcttggaa gatttcccac tttcagtgac
    26281 caacccatac ggtcgtacta agctcatgct agaggaaatt ttgactgata tttacaaagc
    26341 agactcagaa tggaatgttg tcttgcttcg ttactttaac ccaatcggag cccatgagag
    26401 tggtgatttg ggagaaaatc caaacggtat tccaaacaat ctcttgccat atgtgactca
    26461 agtagccgtt ggaaaattag agcaagtgca agtgtttgga gacgattacg atacggaaga
    26521 tggaacaggt gttcgtgact atatccacgt tgtcgatttg gctaagggtc acgttgcagc
    26581 tttgaaaaaa atccaaaaag gttcaggact aaacgtttat aaccttggaa ctggtaaagg
    26641 ttactcagtt cttgaaatta tccaaaacat ggaaaaagcg gtgggccgtc ctattcctta
    26701 ccgcatcgta gaacgtcgcc caggtgatat cgctgcctgc tactcagacc cagcaaaggc
    26761 taaagcagaa ctcggttggg aagcagaact cgacatcacc caaatgtgtg aagacgcatg
    26821 gcgttggcaa agcaagcatc caaatggatt tgaagactaa gatgatgatt tcaatcatcg
    26881 tcccttgttt aaacgaagag gaagtacttc ctctttttta tcaggctctg gaagctttac
    26941 ttccagattt ggaaacagaa atcgagtatg tctttgtcga tgatggatca agtgatggga
    27001 ccttggaact cttaaaggcc tatcgggagc aaaatccggc agtccattat atttctttct
    27061 ctcgaaattt tggcaaagaa gcagcccttt atgcaggctt gcaatatgcg acaggagatt
    27121 tggtggtggt gatggatgca gacctccaag atcctcctag tatgttgttt gagatgaaaa
    27181 atgtactaga caaaaatgta gacttggact gcgttgggac acggagaact agtcgggagg
    27241 gagaaccctt ctttcgcagt ttctgtgctg ttctctttta tcgcctcatg caaaaaatca
    27301 gcccagtagc tctgccgtcg ggtgtccgtg attttcgtat gatgagaagg tctgtagtcg
    27361 atgccatttt aagcttgacc gagtccaatc gtttttcaaa ggggctcttc gcctgggtgg
    27421 gtttcaaaac ctactatttg gactatccaa atgtcgaaag gcaggctggc aagacaagtt
    27481 ggagttttag gcaactcttt ttctactcca ttgaagggat tattaatttt tcagatttcc
    27541 ctttgactat agcctttgta gctggtctcc tatcttgttt tctttctctg ctgatgacct
    27601 tttttgttgt ggttcggacc ctcattttgg gcaatccgac atctggttgg acctctctga
    27661 tggctgttat tctctttctt ggaggcattc aactcttgac cattgggatt ctcggtaagt
    27721 atatcagtaa gatttattta gagactaaaa aaagaccact ttatcttatc aaagaaaaaa
    27781 gtgaccttcc tgattttaca gaaaaaaata aagtgaaaag actataattt tacatggaaa
    27841 tgtgctaaac tagagggagt ggattatcgc cattgattta ggcttcctgg tttccaatca
    27901 gggcgcgagt tgggcaattt ttaacagcct ctaagacatc ctgactagga gaaatttctt
    27961 tttccagttg gtcagggcca tcgtaaaaac gcacgattcc attatcgtgg taatcaaata
    28021 aatcagaata agtttggcaa agcccacagg cgatacatcg ttcaggtata agtgtgattt
    28081 tcatgttcat atttatattg taataagaaa agtaaaaaaa aacaaggagt aaggtatggc
    28141 aaaagaaccg tggcaagaag atatctatga tcaagaagaa tcaagagcag ggcgtcggca
    28201 tcgaaaccac ggaggggctg ataggatggc taatcgtatt ttgacgatcc tagctagtat
    28261 tttctttgta attgtggtgg tgatggtcat cgttctcatc tatctatcat cggggggaag
    28321 taatcgcaca gcagccttaa aagactttca tgattctgat gcaagtgtag tacaaatctc
    28381 atcttcaagc agttctcatc ctgagcagag ttcagagcca gaatctactt ctagtagttc
    28441 agaagaagct gctaatcctg aaggaacgat taaagttctc gcaggagaag gggaagcagc
    28501 tattgccgct cgtgcaggaa tctccattgc tcagttagag gccttgaatc ctgggcacat
    28561 ggctacagga tcttggtttg ctaatccagg tgatgttata aaaataaaat aggagccgat
    28621 atgaaaacaa ttcaaattgc tattgatggt cctgcttcca gcggtaagag tacggtcgca
    28681 aagattattg ctaaggattt tggattcacc taccttgata caggagctat gtatcgtgca
    28741 gcgacctata tggctcttaa gaaccaatta ggagttgaag aagtcgaagc ccttctagcc
    28801 ttgttggacc agcatccaat cagctttgga cgttcagaaa ctggagacca gcttgttttt
    28861 gtaggagatg tggatattac ccatcctatc cgtgaaaatg aagtgaccaa tcatgtttct
    28921 gctattgcag caattcctga agtgcgtgag aaactggttt ctctccaaca agaaattgcc
    28981 cagcaaggcg ggattgtcat ggatggtcgc gatattggaa ctgttgtatt gccacaagca
    29041 gaattgaaaa ttttcctagt agcttctgtt gatgagagag cagagcgtcg ttacaaggaa
    29101 aatattgcca agggaattga aacagacctt gaaaccctaa aaaaggaaat tgctgcgcgt
    29161 gactacaagg atagtcatcg tgagacttct cctctcaaac aagcagagga tgctgtctac
    29221 cttgatacaa ctggtttgaa cattcaagaa gtagttgaaa aaatcaaagc agaagctgaa
    29281 aaaagaatgt aaatgtagaa aagccgatag attgatatcg gtttttttct aaatttgtca
    29341 aaatgctgat tttagggtat aatagttgct gttcgagaaa ttttgatttt caaagaatag
    29401 tgaatgatga taaggagaaa ccatgaacaa tttaccaaat tgtccaaaat gtaactcaga
    29461 gtatgtctac gaagacggtg ccctactggt ttgcccagag tgtgctcatg agtggaatcc
    29521 tgctgaagtt gcagaagtag aagaaggtct tgtcgctatc gatgccaacg gaaataaatt
    29581 gactgatggt gatacagtaa ctctcatcaa ggacttgaaa gtaaaaggtg cgccaaaaga
    29641 tttgaaacaa gggacgcgcg tgaaaaatat ccgcatcgta gaaggcgatc acaatatcga
    29701 ctgtaagatt gatggttttg gtgccatgaa acttaaatca gagtttgtga ggaagattta
    29761 agcatgtcaa aacactataa acttgtattt tatagccgta tcttcttgtt tctagcggct
    29821 tttacgggag tttatcttga aattactagg catggtggtt ttgggatgct tctctattac
    29881 acggttctgt ccaacctctt ggtaactatt tttacccttt atcttctaaa ggttatgagc
    29941 cgtgtaggtg aaaattggca aaggccaagt ctcttgcgct taaaaggtgg ggtcaccatg
    30001 agtatcatga ttacctgtgt gatttaccat ttcctcttgg cgcccattgc gactaatttc
    30061 tataccctag aaaatttcct ttgccactat atcgttccca tctggttttt agcggatacc
    30121 ctcttttttg acaaacaggg tcaatacaag atttgggatc cagcagtgtg gaccatttta
    30181 ccctttctgt atatgatgtt tgctcttttt aatggcttgg ttctaaaact caatattcca
    30241 aatgccaagg ataatccttt cccttacttc tttttgaatg tgaacaaggg ttggaatgtt
    30301 gtgtttaagt ggtgtctgat tatctttgtt gcctatatgg tagcaggatt tattttctac
    30361 tttatcaagc aaatcaagag aaagtcatcc taatactctt cgaaaatctc ttcaaaccac
    30421 gttagtttcc atctgcaacc tcaaaacact gttttgaaca acctgcggct agctttctag
    30481 tttgctcttt gattttcatt gagtatgaga tacaagggat tcattttcga atttaagaag
    30541 gagtctacaa accaatgagg cttcttcttt tcttgcttat ttgataacta tcaaaaaagt
    30601 agaagattaa gagaaatata gaaagagatt tcatgaaaca atttttagaa cgggccagca
    30661 ttttggctct ctccctcgtt ttgattacct ccttttccat ttcgagtgcc ctaccagcca
    30721 tgtttgacta ttatcagggt tattctaagg aacaaattga gctcttggtg agcttgcctt
    30781 cctttggaat catgatgatg ttactgctaa atggtttctt agaaaaaata tttcctgagc
    30841 gcttacagat tagtttgggc ttgctgattt tatcattgag cggtacagct cccttctggt
    30901 accaagccta tccctttgtc tttggaacac ggcttctctt tggtttgggt cttgggatga
    30961 tcaatgccaa ggccatttct attatcagtg aacgctacca aggaaaaagg cgaattcaga
    31021 tgttagggct acgcgcttct gcagaggtcg ttggagcttc tctcattacc ttggccgtcg
    31081 gtcagttgtt ggcctttggt tggacagcta tctttctagc ctatagtgct ggatttttgg
    31141 tgatgcccct ttatctgctc tttgtccctt atggaaaatc aaagaaagaa gtcaagaaaa
    31201 gagcgaagga agcaagtcgt ttaactcgag aaatgaaagg cttgattttt accttagcta
    31261 tcgaagcggc agttgtagtt tgtaccaata cagctattac catccgtatt ccaagtttga
    31321 tggtggaaag aggattgggg gatgcccagt tatctagttt tgttcttagt atcatgcagt
    31381 tgatcgggat tgtggctggg gtgagttttt ctttcttgat ttctatcttt aaagagaaac
    31441 tgctcctctg gtctggtatt acctttggct tggggcaaat cgtgattgcc ttgtcttcat
    31501 ccttgtgggt ggtagtagca ggaagtgttc tggctggatt tgcctatagt gtagtcttga
    31561 cgacggtctt tcaacttgtc tctgaacgaa ttccagctaa actcctcaat caagcaactt
    31621 catttgctgt attaggctgt agtttcggag cctttacgac cccatttgtt ctaggtgcaa
    31681 ttggcttact aactcacaat gggatgttgg tctttagtat cttaggaggt tggttgattg
    31741 taatctctat ctttgtcatg tacctacttc agaagagagc ttaggattga ttcctaagtt
    31801 tttcttttga tactcaatga aaatcaaaga gcatagttga ttgagtttgg aatagtacgc
    31861 tgtagctgct aaaatatttc tagaaattaa tttgactttt ctaatagagt tattcatatc
    31921 ttatttcaat ctactataga aagctagccg caggttgctc aaaatagtgt tttgaggttg
    31981 cagatggaaa ctgacgtggt ttgaagagat tttcgaagag tatgagattt ttatacccat
    32041 acaatatttg ttttaggtcc ttgtttacag actaatttct tgataaaata gaggttatga
    32101 cgagatataa agcaactatt tcctatgatg gttatgcctt tgctggcttt cagcgccagt
    32161 ctcatgcgcg tagcgttcag gaagaaattg aaaaaacctt gaccagatta aataaagggc
    32221 aaaccattac tgttcacggt gctggtagga cagatagtgg ggttcatgcc ctgggacagg
    32281 tcattcattt tgacctgcct tatcagatgg atgaggagaa actccgtttt gccttggata
    32341 cccagtctcc tgaagatatt gatgtgattt cgattgagct tgtggcagat gattttcatt
    32401 gccgttatgc caaacatagc aagacctatg agtttattgt ggatagagga cgtcccaaaa
    32461 atcctatgcg ccgtcactat gccacccact ttccctaccc actcgatgtg gaacgaatgc
    32521 agattgcaat caaaaagcta gagggaaccc atgattttac cggttttaca gcctctggga
    32581 ctagtgtaga ggataaggtt cgcaccatca cagaagctag tttaatagtc gatgagacag
    32641 gacaattttt gacctttacc ttttcaggaa atggtttctt gtataaacag attcgcaata
    32701 tggtggggac tcttctcaaa atcggtaaca accgcatgcc agtagagcag attgatctca
    32761 tcttggagaa gaaggacagg caacttgcag gtcccactgc agcaccaaat ggtttgtatt
    32821 taaaggagat tcgttatgaa gaataatcgt attttagcac tttctggaaa tgatattttt
    32881 agtggtggtg gactgtcagc tgatttggct acctatacct tgaacggctt gcatgggttt
    32941 gtagcagtga cttgtttgac agccttgaca gaaaaaggat ttgaagtctt tccaactgat
    33001 gataccattt ttcaacatga attagatagc ttacgtgatg tggaatttgg gggaattaag
    33061 attggtcttc tccctactgt cagtgtggct gagaaggcct tggactttat caaacaacgc
    33121 ccaggagtac ctgtggtgtt ggatcctgtc ttggtctgca aggaaacgca tgatgtagct
    33181 gtcagtgagc tctgccaaga gttgattcgc tttttccctt atgtcagtgt gattacgcct
    33241 aatctcccag aagcagaatt attatccggt caggaaatta aaaccttgga agacatgaaa
    33301 actgcagcgc agaaattgca tgatttagga gcgccagcag tcattatcaa gggaggcaat
    33361 cgtcttagtc aggacaaggc tgtggatgtc ttttatgatg gacagacctt tactatccta
    33421 gaaaatccag ttatccaagg ccaaaatgtt ggtgcaggtt gtacctttgc ctctagcatt
    33481 gccagtcact tgattaaagg tgataaactt ttgccagcag tagaaagctc taaggctttc
    33541 gtttatcgtg ctattgcaca agcagatcag tatggagtaa gacaatatga agcaaacaaa
    33601 aacaactaaa atcgcccttg tatccctatt aaccgccctt tctgtggttc taggttattt
    33661 cttaaaaatc ccaacaccta caggaattct aactctttta gatgctggtg tcttctttgc
    33721 ggccttttac tttggtagtc gtgaaggagc ggtagtcgga ggactagcaa gtttcttgat
    33781 tgacctctta tcaggctacc ctcagtggat gttctttagc ttggtcaacc atggcttgca
    33841 gggatttttc gcaggattta aaggaaaaag tcagtggtta ggccttattt tagcaactat
    33901 tgccatggta ggaggctacg ccttgggttc tactttgatg aatgactggg cagcagccct
    33961 cccagaaatt ctaccaaatt ttatgcaaaa tatggtaggg atgattgtag gatttattct
    34021 tagtcaaagt atcaagaaga ttaagtaata ctcttcgaaa atctcttcaa accacgtcag
    34081 cttccatctg caacctcaaa acactgtttt gagcagcctg aggctagctt tctagtttgc
    34141 actttgattt tcattgagta taaagaggtt gagtaaaaag tctttcaaat aagaaaaacg
    34201 catagtatca ggtgttgaat gaactgc
//
LOCUS       DAVKDZ010000016        33856 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000016 DAVKDZ010000000
VERSION     DAVKDZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33856
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.016"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            145..543
                     /gene="spx"
                     /locus_tag="VWZ30_000729"
     CDS             145..543
                     /gene="spx"
                     /locus_tag="VWZ30_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEU9334511.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            609..1178
                     /locus_tag="VWZ30_000730"
     CDS             609..1178
                     /locus_tag="VWZ30_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9334512.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGSATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSNYISDRLANGATEQ"
     gene            1265..1531
                     /locus_tag="VWZ30_000731"
     CDS             1265..1531
                     /locus_tag="VWZ30_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEU9334513.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            1535..1954
                     /gene="ruvX"
                     /locus_tag="VWZ30_000732"
     CDS             1535..1954
                     /gene="ruvX"
                     /locus_tag="VWZ30_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU9334514.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            1970..2275
                     /locus_tag="VWZ30_000733"
     CDS             1970..2275
                     /locus_tag="VWZ30_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEU9334515.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            2582..3832
                     /locus_tag="VWZ30_000734"
     CDS             2582..3832
                     /locus_tag="VWZ30_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9334516.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            3829..4374
                     /locus_tag="VWZ30_000735"
     CDS             3829..4374
                     /locus_tag="VWZ30_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEU9334517.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKSIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDID
                     GTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVN
                     GNQIVVDDMDRDPSDQIVLTK"
     gene            4520..6052
                     /gene="cls"
                     /locus_tag="VWZ30_000736"
     CDS             4520..6052
                     /gene="cls"
                     /locus_tag="VWZ30_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU9334518.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            6130..6273
                     /locus_tag="VWZ30_000737"
     CDS             6130..6273
                     /locus_tag="VWZ30_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334519.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            6310..7848
                     /locus_tag="VWZ30_000738"
     CDS             6310..7848
                     /locus_tag="VWZ30_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9334520.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSFLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            7968..10175
                     /gene="nrdD"
                     /locus_tag="VWZ30_000739"
     CDS             7968..10175
                     /gene="nrdD"
                     /locus_tag="VWZ30_000739"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEU9334521.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLDDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKQEDYAWK
                     KAQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            10187..10327
                     /locus_tag="VWZ30_000740"
     CDS             10187..10327
                     /locus_tag="VWZ30_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334522.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            10371..10877
                     /locus_tag="VWZ30_000741"
     CDS             10371..10877
                     /locus_tag="VWZ30_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9334523.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDVEDFSYEEWL
                     EGNRNKEMGIWLPENRVPSIQFVLFGESGCALGFLNLRLRLNEGLLNYAGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            10870..11460
                     /gene="nrdG"
                     /locus_tag="VWZ30_000742"
     CDS             10870..11460
                     /gene="nrdG"
                     /locus_tag="VWZ30_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU9334524.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            11457..12074
                     /locus_tag="VWZ30_000743"
     CDS             11457..12074
                     /locus_tag="VWZ30_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEU9334525.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            12341..12649
                     /gene="rpsJ"
                     /locus_tag="VWZ30_000744"
     CDS             12341..12649
                     /gene="rpsJ"
                     /locus_tag="VWZ30_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU9334526.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            12866..13492
                     /gene="rplC"
                     /locus_tag="VWZ30_000745"
     CDS             12866..13492
                     /gene="rplC"
                     /locus_tag="VWZ30_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU9334527.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            13517..14140
                     /gene="rplD"
                     /locus_tag="VWZ30_000746"
     CDS             13517..14140
                     /gene="rplD"
                     /locus_tag="VWZ30_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU9334528.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            14140..14436
                     /locus_tag="VWZ30_000747"
     CDS             14140..14436
                     /locus_tag="VWZ30_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU9334529.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            14454..15287
                     /gene="rplB"
                     /locus_tag="VWZ30_000748"
     CDS             14454..15287
                     /gene="rplB"
                     /locus_tag="VWZ30_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU9334530.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSCWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            15391..15672
                     /gene="rpsS"
                     /locus_tag="VWZ30_000749"
     CDS             15391..15672
                     /gene="rpsS"
                     /locus_tag="VWZ30_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU9334531.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            15684..16028
                     /gene="rplV"
                     /locus_tag="VWZ30_000750"
     CDS             15684..16028
                     /gene="rplV"
                     /locus_tag="VWZ30_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU9334532.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            16041..16694
                     /gene="rpsC"
                     /locus_tag="VWZ30_000751"
     CDS             16041..16694
                     /gene="rpsC"
                     /locus_tag="VWZ30_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU9334533.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            16698..17111
                     /gene="rplP"
                     /locus_tag="VWZ30_000752"
     CDS             16698..17111
                     /gene="rplP"
                     /locus_tag="VWZ30_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU9334534.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            17121..17327
                     /locus_tag="VWZ30_000753"
     CDS             17121..17327
                     /locus_tag="VWZ30_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU9334535.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTVRLKEVKKQIARIKTVQSEAK"
     gene            17352..17612
                     /gene="rpsQ"
                     /locus_tag="VWZ30_000754"
     CDS             17352..17612
                     /gene="rpsQ"
                     /locus_tag="VWZ30_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU9334536.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            17638..18006
                     /gene="rplN"
                     /locus_tag="VWZ30_000755"
     CDS             17638..18006
                     /gene="rplN"
                     /locus_tag="VWZ30_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU9334537.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            18084..18389
                     /gene="rplX"
                     /locus_tag="VWZ30_000756"
     CDS             18084..18389
                     /gene="rplX"
                     /locus_tag="VWZ30_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU9334538.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            18413..18955
                     /gene="rplE"
                     /locus_tag="VWZ30_000757"
     CDS             18413..18955
                     /gene="rplE"
                     /locus_tag="VWZ30_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU9334539.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            18973..19242
                     /gene="rpsN"
                     /locus_tag="VWZ30_000758"
     CDS             18973..19242
                     /gene="rpsN"
                     /locus_tag="VWZ30_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU9334540.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            19456..19854
                     /gene="rpsH"
                     /locus_tag="VWZ30_000759"
     CDS             19456..19854
                     /gene="rpsH"
                     /locus_tag="VWZ30_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU9334541.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            20046..20582
                     /gene="rplF"
                     /locus_tag="VWZ30_000760"
     CDS             20046..20582
                     /gene="rplF"
                     /locus_tag="VWZ30_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU9334542.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VDGTEVTLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            20666..21022
                     /gene="rplR"
                     /locus_tag="VWZ30_000761"
     CDS             20666..21022
                     /gene="rplR"
                     /locus_tag="VWZ30_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU9334543.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            21040..21534
                     /gene="rpsE"
                     /locus_tag="VWZ30_000762"
     CDS             21040..21534
                     /gene="rpsE"
                     /locus_tag="VWZ30_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU9334544.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            21506..21730
                     /gene="rpmD"
                     /locus_tag="VWZ30_000763"
     CDS             21506..21730
                     /gene="rpmD"
                     /locus_tag="VWZ30_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU9334545.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            21832..22272
                     /gene="rplO"
                     /locus_tag="VWZ30_000764"
     CDS             21832..22272
                     /gene="rplO"
                     /locus_tag="VWZ30_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU9334546.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            22285..23595
                     /gene="secY"
                     /locus_tag="VWZ30_000765"
     CDS             22285..23595
                     /gene="secY"
                     /locus_tag="VWZ30_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU9334547.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDKTE"
     gene            23746..24384
                     /locus_tag="VWZ30_000766"
     CDS             23746..24384
                     /locus_tag="VWZ30_000766"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU9334548.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMVNQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGVINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            24501..24719
                     /gene="infA"
                     /locus_tag="VWZ30_000767"
     CDS             24501..24719
                     /gene="infA"
                     /locus_tag="VWZ30_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU9334549.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            24744..24860
                     /gene="rpmJ"
                     /locus_tag="VWZ30_000768"
     CDS             24744..24860
                     /gene="rpmJ"
                     /locus_tag="VWZ30_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU9334550.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            24878..25243
                     /gene="rpsM"
                     /locus_tag="VWZ30_000769"
     CDS             24878..25243
                     /gene="rpsM"
                     /locus_tag="VWZ30_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU9334551.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            25261..25644
                     /gene="rpsK"
                     /locus_tag="VWZ30_000770"
     CDS             25261..25644
                     /gene="rpsK"
                     /locus_tag="VWZ30_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU9334552.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            25687..26622
                     /locus_tag="VWZ30_000771"
     CDS             25687..26622
                     /locus_tag="VWZ30_000771"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU9334553.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            26634..27020
                     /gene="rplQ"
                     /locus_tag="VWZ30_000772"
     CDS             26634..27020
                     /gene="rplQ"
                     /locus_tag="VWZ30_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU9334554.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            27285..27551
                     /locus_tag="VWZ30_000773"
     CDS             27285..27551
                     /locus_tag="VWZ30_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU9334555.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            27561..28898
                     /locus_tag="VWZ30_000774"
     CDS             27561..28898
                     /locus_tag="VWZ30_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEU9334556.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSAVVQKGYQKGDEILINSIPRALTETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGAAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMRVNGASSVDFISRGGQIPAPIHSFKN"
     gene            29142..29834
                     /locus_tag="VWZ30_000775"
     CDS             29142..29834
                     /locus_tag="VWZ30_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9334557.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEGGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(29937..31582)
                     /locus_tag="VWZ30_000776"
                     /pseudo
     CDS             complement(29937..31582)
                     /locus_tag="VWZ30_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(31572..31877)
                     /locus_tag="VWZ30_000777"
                     /pseudo
     CDS             complement(31572..31877)
                     /locus_tag="VWZ30_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809465.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
     gene            complement(32013..32650)
                     /locus_tag="VWZ30_000778"
                     /pseudo
     CDS             complement(32013..32650)
                     /locus_tag="VWZ30_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862596.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(32663..33784)
                     /locus_tag="VWZ30_000779"
                     /pseudo
     CDS             complement(32663..33784)
                     /locus_tag="VWZ30_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931989.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
BASE COUNT        10518 a         6204 c         7515 g         9619 t
ORIGIN      
        1 gatagtcatt tagttgtttg tcaagaaaaa agtacgaaaa tgacgaaaaa agtcaaaaaa
       61 aattaaaaat aggtcgcaag tcggatgttt tttatggtat aatagactaa actgatagta
      121 acatgtagcg aaaggggtag gtacatgatt aaaatttata cagtctcaag ttgtactagc
      181 tgtaaaaaag caaaaacctg gctcaatgcc caccagttaa gttataaaga acaaaacctt
      241 ggtaaagaag gaattacgag agaagaatta ctggatattc taaccaaaac agataacgga
      301 atagccagca tcgtttcgtc taaaaatcgc tatgccaaag cccttggagt ggatattgaa
      361 gatttgagtg tcaatgaagt tctcaatctg attatggaaa caccgagaat tttaaagagc
      421 ccaatccttg tagatgaaaa acgtctgcaa gttggctaca aggaagacga tattcgtgcc
      481 ttcctaccac gctctgtccg taatgtagaa aatgcagaag cacgtttgcg tgcagctcta
      541 taaacatcaa ggctgggagc acttcccagt cttattctat tttaatttca aaaagaaaga
      601 agaaagaaat gaaaaaaata gttcttgtta gtctagcttt cctttttgtc ctggttggtt
      661 gcggacagaa aaaagaaact ggatcagcta caaaaacaga aaaagatacg cttcagtcgg
      721 cattgccagt tattgaaaat gctgagaaga atacagttgt aactaagact ttggtcttgc
      781 ccaagtcaga tgatggtagc cagcagacac aaacaattac ttacaaggac aagacttttt
      841 tgagtctaac tatccaacaa aaacgtccag tctctgatga gttgaagact tatattgacc
      901 aacatggagt ggaggaaact caaaaagctc ttcttgaagc ggaggagaag gataagtcta
      961 tcattgaagc tcgtaaattg gcaggtttca aacttgaaac aaaactattg agcgcaacgg
     1021 aacttcaaac aacgactagt tttgattttc aagttctgga tgtcaagaag gcttcccagt
     1081 tggaacatct gaagaatatt ggtttggaaa atcttttgaa aaatgaacca agcaactata
     1141 tttcagatag attggcaaat ggcgcgacag aacaatagaa aatccaaata aatagactgc
     1201 ctgaattgta gaaggtaagg aattatgcta taatggtact attctaagga aagagggtgt
     1261 tagaatggga tttactgaag aaacagtacg ttttaaattg gacgattcca ataaaaaaga
     1321 aattagcgaa actttgacag atgtttatgc ttcgttgaac gataagggtt acaacccaat
     1381 taaccaaatc gtaggttacg tattgagtgg agaccctgcc tacgttcctc gttataataa
     1441 tgcacgaaat caaatccgta agtatgagcg tgatgaaatc gttgaggaat tggttcgtta
     1501 ctacctcaaa ggacaaggag tcgatctata acctatgaga attatgggat tggacgtcgg
     1561 ttcaaaaacg gtaggggtgg cgattagcga tccgcttggt tttacagctc aagggcttga
     1621 aatcatccag ataaatgaag aacaaggcca atttggtttt gaccgcgtta aggaattggt
     1681 tgatacttac aaggtggaac gatttgtagt gggcttgcct aaaaacatga acaatacaag
     1741 tggaccgcgc gtagaagcta gtcaagccta cggagcaaag ctagaagagt tttttggttt
     1801 accagtagac tatcaggatg aacgcttgac aacagtggct gctgagcgca tgttgattga
     1861 acaagcagat atcagtcgca ataagcgcaa gaaagtcatt gataagttag cagctcagct
     1921 gattttacaa aattatttag atagaaaatt ttaatataaa ggagaggcta tgtcacacga
     1981 tcataaccac gaccacgaag aacgtgaact aatcacacta gtagatgagc aaggaaatga
     2041 aaccttgttt gaaatccttt tgacaattga tggaaaagaa gaatttggta aaaactatgt
     2101 tcttctagta ccagttaacg cagaagaaga cgaagacgga caagttgaaa tccaagctta
     2161 ctcattcatc gaaaacgaag atggaacaga aggcgaattg caaccaatcc cagaagactc
     2221 agaagacgaa tggaacatga ttgaagaagt cttcaacagc tttatggagg agtaaaaacg
     2281 tccgggggac gtttttagcc ctgcgctaga aattagaaac gcaggtagtc tgggagactg
     2341 tatcagccca actcccagaa atggggaaga gttggaacgt ccagtgaaca tttttacccc
     2401 aactcttaga aattgagaag agttggaaca tctagtggat gtttttagcc catattcaca
     2461 ttcatagtag ctagttatta gctaaccagg taagaggtcg ggacgaaaat cctggccttg
     2521 tttttattag taaccatact ttaatgcgat aattgattgg acttttgtca aggagatgtg
     2581 tatgtttgaa gtagaagaat ggctccatag tcggattggt ttgaattttc gatcaggttt
     2641 gggccgaatg cagcaagcgg tggatttgtt aggaaatcct gagcagtctt accctattat
     2701 ccacgtaaca gggactaatg ggaaaggatc taccattgct tttatgaggg aattatttat
     2761 ggggcatggc aaaaaagttg cgacctttac ctcccctcat atcgtctcta tcaatgaccg
     2821 aatctgcatt aatgggcaac ctatagcaga cgcagacttt atccgtttgg ctgatcaggt
     2881 caaggagatg gagaaaacgc ttctgcaaac tcctgaccag ttgtcctttt ttgaattgct
     2941 gaccttagtt gcttttcttt attttaggga gcaagaggtg gatttggttt tattagaagt
     3001 gggaattggt ggcttacttg acacgaccaa tgtggtaact ggagagcttg ctgtcatcac
     3061 ctccattggg cttgaccatc aagaaacctt gggtgatagt ctagaagcaa ttgcagagca
     3121 gaaagctggt attttcaagg ctggtaagaa ggcagtgatt gcgaaattgc ctccagaagc
     3181 taggcttgtc tgtcagaaaa aagccgaatc tttagctgtt aacctttatc aggcaggtca
     3241 agatttttta atgctgaatg gtgatttttc aagctcttta ctaaatcttt cacagctgaa
     3301 cataggctta gaaggagtct atcagcagga gaatgcagcc ttggcgttgc aaacttttct
     3361 tctttttatg ggagaaggaa aggaagctgt tgatgaacag gctgtaagaa aggccttgga
     3421 acagacccat tgggctggtc gcttggagcg tattcgccca cagatttatt tggatggtgc
     3481 tcataacctc cctgccttga ctcgcttggt tgagtttatc aaagaaaaag agcaggaagg
     3541 ttatcgacct caaatcctct ttggatcctt gaaacgtaag gattatcaag ggatgttggg
     3601 ttatctgact gaaaatttac ctcaagtgga actcaagatg actggctttg actatcaagg
     3661 gtctttggac gagacagatg taacaggtta ccatgtaatt ccctcttacc gagaatttat
     3721 cagcagtttc gaagaaaggg cagacgctca agacttgctg ttcgtaacag ggtctctcta
     3781 ttttatctca gaagtacggg gctacctgct ggaccgtgag cagataaatt gacccccttt
     3841 tttgaacttg ttatactagg ggaattgctg gttagagaaa atttctctaa attggtagca
     3901 gaaaggaaat ccatcatgaa attaaaaaga ttcacacttt ctcttgcttc tctagcaagt
     3961 tttagtctct tagtagcttg ttcacaaaga gctcaacagg ttcaacagcc tgttgctcag
     4021 cagcaggtcc aacagcctgc tcaacagaat accaatactg caaatgcagg gggtaaccaa
     4081 aatcaagcgg ctccagtaca aaaccaacct gttgctcagc cgaccgatat tgatgggact
     4141 tatactggtc aggatgacgg agaccgtatc actttagtgg taactggaac gactggtaca
     4201 tggactgagc tcgaatctga cggggatcag aaagtcaaac aggttacatt tgattcagca
     4261 aatcaacgca tgattattgg cgatgatgtc aaaatttaca ctgtaaacgg taatcaaatc
     4321 gtcgtagatg atatggatag agacccatcg gaccaaatcg ttttaactaa ataagactat
     4381 gcaagtagaa agggctggat ttttcagcct ttttactttt acctagaaaa taatcataaa
     4441 tacttgcctt ctaccgaata cctgcgttat actagtatca attacttgtt tttagcattc
     4501 aaactatcaa ggaggggata tgaaatatag gaaatttcaa ttattgatgt ccaagtatgg
     4561 ctttagtctt tcgattatgc tacttgaact ttgtctggtt tttggtctct ttctttattt
     4621 aggacgcatg gctcccattt tatggattac tgtcctcatt ctactgagta tcattacaat
     4681 catttcgata gtcaaccgta atacgactcc tgagaataag gtaacctggt tgttagtagc
     4741 ctttgtgcca gtatttggcc ccttgctcta tctgatgttt ggtgaaaggc gattgtcaaa
     4801 aaaagaaatc aaacaactga agaagctagg ctccatgcat ttccaagaag caaatagcca
     4861 gctactaaaa gagaaattaa aagaaagtga caaggcagct tatggagtca tcaagtcctt
     4921 attgagtatg gataccaatg ctgacatcta tgatcaaact gcctctacat tttttcctaa
     4981 cggagaagct atgtggaaaa agatggtaga agatcttaaa aaggctgaga aatttatttt
     5041 cttggaatat tacattatag aagaaggttt gatgtggaat cgcatactag atatactaga
     5101 gcaaaaggta gctcagggtg tagaggttaa gatgctctat gatgatatcg gctgtatggc
     5161 tactttaaca ggagattatg cacatcgact tcgtcagctg ggcatcgagg cccataaatt
     5221 caataaagtt attcctcgtt tgacagtggc ttataataac agagatcata gaaaaatatt
     5281 gattgttgat ggtcagatag cctatactgg tggggtcaat ctggcagatg agtacattaa
     5341 ccacgtcgag agatttggtt attggaagga tagtggaatt cgcttagacg gactagcagt
     5401 aaaagccctg acacgcttat ttttgaccac ttggtacatt aatcgaggag aaattagtga
     5461 ttttgatcaa tatcatttag aaaatcattc tatcccgagt gacggtttaa ccattccata
     5521 cggaagtgga cccaagccaa tttttcgagc gcaggtaggg aaaaaagttt atcagagttt
     5581 aatcaatcaa gcaacggaat cggtctatat tacgacacct tatttgatta tagattatga
     5641 tttaacagag acaatcaaaa atgcagctat gagaggggtc gatgttcgaa ttatcacccc
     5701 ttacatacca gataagaagt tcattcagtt agtcacgaga ggagcttatc ccgaccttct
     5761 ttctgctggt gttcggattt atgagtatag tccaggtttt attcatagta agcagatgtt
     5821 ggtagacgaa gattttgcgg tggtggggac aatcaatctc gactaccgga gcttggtaca
     5881 ccattatgaa aatgcagtct tactctataa aactccttct ataagggaaa tcgcccgaga
     5941 ttttcgaaat atatttgcag attctcagga agtctatcct cattctatca aaacgagctg
     6001 gtatcaaaag cttgtaaaag aaatcgccca gctattcgcc cctatcttat aagaaatcta
     6061 gaacttagga tacaaaccag acctattttt cttgtctttt acgaatagaa agaaagagga
     6121 ggggaattca tgctaactaa ggaagaagtg aatgatatga ttgagttcaa acagacacat
     6181 ttacaccgct ttcgggaaat tgaaaccttt gtgaaactat gcaaaaaatc actcaaacag
     6241 ccatcgcaag ctgacaatcc taggactttt tgatatacta agacagataa attgaacagg
     6301 agaaacgata tgagtgtgta tggtagagta gaagaagttc ataaggagaa tcgtgaacct
     6361 ctagaatacc aaatcgaaca agaatcgcat catcgtgaat caagtcgtct tcctttggtg
     6421 aaaattttac tatggagtac gcttgtaacg gggataactc taggagtacc gctattactc
     6481 gacttaatga gtgcacaaga agtgcaggat ttttatgcag gttgggcgct tcatcagaca
     6541 gggaagattt acagcgacta ttatggaagt caaggtttgc tttattattt gctgacttac
     6601 gtgagtcagg gcggattttt ctttgccatc tttgagtggt tagccttggt agcaggagga
     6661 tttttccttt ttagatcagc ggacaccttg acagagcaag gagaccaagc tggacatctg
     6721 gtgactattt tttacatgct agttacaggt cttgcttttg gtggaggcta tgcgactctt
     6781 ttagcgcttc ctttcttatt cgcagccttt agtttagttg cggcttacct aagcaatcca
     6841 agccatgata agggatttgt acggattggg ctagctttgg caggcggatt tttctttgct
     6901 cccttatcat cgttcctgtt tattgctgta gtgagtttag gcttgttggt ctttaacctt
     6961 gggcatagac gctttgcgca tgggttttat cagtttcttg cagtggcttt aggtttttca
     7021 cttgtctttt atccaactgc ctactatagt gctgcaacag gaagttttgg ggatgcgatt
     7081 agtggtattc gttatcctat tgacagtatt cgctttgatt ttacttctaa aattttagag
     7141 aatatgtttt tttatggctt gttgtccctt ggtttgggat ttgtggtttg tatcttttta
     7201 ggtctctttc aatccaaacc ctttaaacta tacgtcattt cggttcctgc aagtcttgtg
     7261 gtaattttag gtttgatttt acttttcttt tcacaagagc ctctgcacgc ttcttatttg
     7321 atggtcgtct tccctgtttt cctactttta ttggtaacca atattaagag tcaacagagg
     7381 gggcgtagtg ctagaagaag ccgaagagaa acgccagtta gcctatggag tcgtttcttc
     7441 aaaggaaatc tatatctgct agtttttggg tttgtctatc ttttgtctgt tccttttttg
     7501 atgaagtttg tcctttatcc agtaccttat caagaacgta atcgtcttgc tgatttggta
     7561 aaagaggaga caaatacgga agatgctatc tatgcatggg atgatactgc gactctttat
     7621 cgtaagagtg agcgcttgtc gccatcggcg attttgtccc cgttgcacta tacagcaact
     7681 gaggaaaatc gtaataagtt acttaatgac ttgaaagaaa aacaacctaa ggtgattgtg
     7741 gtaaatgata aggtggtagt ctggtctgaa gtggaaacac tcttaaaaga aaattaccaa
     7801 caagtaaaga ctgattactc agagtttaaa gtctataaaa ttaaataacc aaatcaatat
     7861 cttgtgtatt tttaaaaatt ttaggatttt taacacaaga tattgatttt tctttttaga
     7921 gtggtataat actttttaga aagaacattt tagaaaagag catgcatatg attgcactag
     7981 aagaaaaaat tacaattttg ccaactctct tcgtcgagaa acgagatggg agacgtgttg
     8041 tatttgatgt ggacaagatt gacaaggctc tccacaaggc ggctgacaag gttatggatg
     8101 tgacacccct ggttgaaaaa cgcctcgatg atctgactga gcgaattatt acagaaattc
     8161 atagtcgctt tccacaggga attaagattt acgaaattca aaatatcgta gaacatgaac
     8221 tccttgaagc caaagaatat gcgctggctg aggagtatat tacttatcgg acacagaggg
     8281 attttgagcg ctcaaaagcg acggatatca actttagtat tcataaactt ctcaacaaag
     8341 accagacagt tgtcaatgaa aacgctaata aagacagtga tgtctttaac actcagcgtg
     8401 atttgacagc agggattgtt gggaaatcaa tcggactgca aatgcttcct aagcacgtag
     8461 ccaatgccca ccaaaagggg gatatccact atcacgattt ggactacagt ccctataccc
     8521 ctatgaccaa ctgctgtttg attgatttta agggtatgtt ggaaaatggt tttaagattg
     8581 gaaatgcaga ggtagagagt cccaagtcta tccagactgc gacagcacag atttctcaaa
     8641 tcattgccaa cgttgcttct agccagtacg gtggctgttc agctgaccgt atcgatgaaa
     8701 ttttggcgcc ttatgcagag aagaattatc aaaaacatct caaagatgca gaagagtggg
     8761 tattgcctga aaaacaggaa gattacgctt ggaagaaagc gcaaaaggac atctacgatg
     8821 ccatgcaatc tcttgagtat gaaatcaata ctctcttcac ttcaaatgga caaacacctt
     8881 ttacttcgtt aggttttggt ctgggaacca gtcgttttga acgagaaatt caaaaagcta
     8941 ttttaaacat tcgcatcaag ggtcttggtt cagaacaccg tacggctatc tttcctaaac
     9001 ttatctttac gcttaaaaga ggcctcaact tagaggaagg aactcccaac tatgacatca
     9061 agcagttggc tctagagtgt gcaaccaagc ggatgtatcc agacgtcttg tcttatgata
     9121 agattgttga tttgacaggt tctttcaagg tgcctatggg ctgccgttct ttccttcaag
     9181 ggtggaagga tgaaaatggt gtagaagtca attcaggtcg catgaatctg ggtgttgtga
     9241 cggttaatct gcctcgtatt gctcttgagt ctgaaggtga tatgaataag ttctgggaaa
     9301 tcttcaacga gcgaatgaat atcgcagaag atgctcttgt ttaccgtgtc gaacgcacta
     9361 aagaggcgac accagcgaat gctcctattc tttatcagta cggtgctttt ggccatcgtc
     9421 taggtaaaga agaaagtgtt gaccagctct ttaagaatcg tcgtgcgacc gtttcgctgg
     9481 gctatatcgg cttgtatgaa gtagcgacag ttttctttgg taacagctgg gaaagtaatc
     9541 cagatgctaa ggaattcacg ctagacatca ttcacgatat gaaacgccgt gtagaagagt
     9601 ggtcagacca atatggctac catttctcta tctactcaac accatccgaa agtctgacag
     9661 accgtttctg ccgactagat atagacaagt ttggctctat tcctgatatc acagacaagg
     9721 aatactacac caattctttc cactacgatg ttcgtaaaaa tccaacaccg tttgaaaaat
     9781 tagactttga gaaagtctat ccggaagcag gtgcgtcagg tggtttcatc cattattgtg
     9841 agtatccagt ccttcagcaa aatccaaagg ccttggaagc tgtctgggat tatgcttatg
     9901 accgtgtagg ctatctaggc accaatactc cgattgaccg ttgctacaag tgtgactttg
     9961 aaggggattt tgaaccaact gagagagggt ttgcttgtcc aaactgtggc aatagcgatc
    10021 ctaaaacagt agacgttgtt aaacggactt gtggctatct tggcaatcct caagcgagac
    10081 caatggttaa cgggcgtcac aaggaaatcg ctgcgcgtgt caaacatatg aatggttcaa
    10141 cgattaaaat agctgggcat caagtaacaa attagaaaga aatgaaatgg gaaaatatca
    10201 actagacgat aaggggcgcg cacaagtgac ccgttatcac gagaagcact ctaaaggtgg
    10261 agctggtaag aaagaacgct tgcttagctt cagagaacaa tttttaaaca agaacaagaa
    10321 aaaataaaag tgagagccag ctctcgcttt tctcatagtg ggaggtaggg atggaattac
    10381 gcagaccaag attagcggat aagaaagctg ttttagatat gatgacagag tttgaaaaga
    10441 gccaatcagc ccatgatgga ggattttggg atgttgagga tttttcttac gaggagtggc
    10501 tagaaggtaa ccgtaataaa gaaatgggaa tatggctacc tgaaaatcgc gttccatcga
    10561 tacagttcgt attgtttggt gaatcaggtt gtgctctagg atttttgaat ctgcgattga
    10621 gactgaatga ggggttactg aattatgctg gccacattgg ctactccatt cgtccatctg
    10681 aaagaggcaa gggttatgca aaagagactc tccgtcaggg cttgcaagtt gctaaggaaa
    10741 agaacatcaa gaaagctctg gtgacctgta gtgtgaataa tcctgctagc agagcagtca
    10801 ttctagcaaa tggtggaata tttgaggatg ctcgcaatgg agtcgagcgt tattggatag
    10861 aggtagcgaa tgaataatcc aaaaccacaa gaatggaaaa gcgaggaact tagtcaaggt
    10921 cgtatcattg actacaaggc ctttaacttt gtggacggcg aaggcgtgcg caactctctc
    10981 tatgtatcag gctgtatgtt tcactgcgag ggatgttata atgttgcgac ttggtctttt
    11041 aatgctggca ttccctatac agcagaatta gaagagcaga ttatggcaga ccttgcccaa
    11101 ccctatgttc aaggcttgac tttgctggga ggggagcctt ttctcaatac tggcattctc
    11161 ttgcctctag ttaaacgcat ccgaaaggaa ttgccagaca aggacatttg gtcctggacg
    11221 ggctacactt gggaagaaat gatgttggaa actccagata aactggaact cttatcattg
    11281 attgacattc ttgtcgatgg acgatacgat cgaactaaga gaaatctcat gctccagttt
    11341 cgaggttcgt ccaaccaacg aattatcgat gtgcaaaaat cgctcaaaag tgggcaagta
    11401 gtgatttggg acaagctcaa tgacggaaaa gaaagctatg aacaggtgaa gagagaatga
    11461 agaaaaagga cttagtagac caactagtct cagagatcga gacggggaaa gtcaggacac
    11521 tgggaatata cggtcatgga gcttcaggta aatcaacctt tgcacaggaa ttgtaccaag
    11581 ctttagattc tactacagta aatttgctag agacagatcc ttatatcacc tcaggacgcc
    11641 atctggtagt acccaaggac gcgccgaatc aaaaggtgac agccagtctg ccagtggcgc
    11701 atgaactgga gagtttgcag agagatatcc ttgccttgca ggcgggtatg gatgtcttga
    11761 caattgaaga accttggaag gctagtgagg tcttgtctgg agccaaacca attttgattg
    11821 tcgaagggat gtctgttggc tttctaccca aggaactctt tgaaaaaacc atctgtttct
    11881 acacggatga ggagaccgaa ttaaagcgac gccttgctag agatacgact gtgagaaatc
    11941 gcgatgcatc ctttatattg gctagccatc agatgagacg ggagcagtat ctgcgatact
    12001 ataaagaaac tgagtctaag gcggatatct tagtggacca atcagaagat aaatttgatg
    12061 tcaagaggac ttaacttatg tatagaagaa atatagaaga aaatccctaa ttttagaaag
    12121 aaaaagtagg aaaacagttt catcccaaaa aaacgaaaaa acagcaacaa atcccttgca
    12181 atcgcagggg ctttgtgtta ttctattatg gtgctgtaaa ttacagcctt agttttgatg
    12241 caagaggttg cgacacgctc ggttgcattg ccacgcaaca ccgcgtcggt tttcttgtgg
    12301 agctagccta ttatcttaaa tagacgaaaa ggagaaaaag atggcaaaca aaaaaatccg
    12361 tatccgtttg aaagcttacg aacaccgtac gcttgacaca gcggctgcaa aaatcgtaga
    12421 atcagctact cgtacaggtg cacaagttgc gggtccaatc ccacttccaa ctgaacgtag
    12481 cctctacaca atcattcgtg cgactcacaa atacaaagac tctcgcgaac aatttgaaat
    12541 gcgtacacac aaacgtttga tcgatatcgt taacccaact caaaaaacag ttgatgcctt
    12601 gatgaaattg gatcttccaa gtggtgtaaa cgtagaaatc aaactttaat ctaaaatata
    12661 aaagagcaga ggctggtgtt tcaatctaat tgaacacgga ctaaactcgg tgtgaaaaag
    12721 ataaacttcc tagtgtctgc tagacactgc gtcagtttcc tattttcacg ttgagtttga
    12781 cgccctttgt atcttagact tgagcataaa aaacgctcgt taaaaacttt ttgaataaaa
    12841 aatatagaaa aggaactatt ttctcatgac aaaaggaatc ttagggaaaa aagtgggaat
    12901 gactcaaatc ttcactgaag ctggcgaatt gatccctgta acagttattg aagcaactcc
    12961 aaacgttgtt cttcaagtta aaactgttga aacagacgga tacaacgcta tccaagttgg
    13021 tttcgatgac aaacgcgaag tattgagcaa caaacctgct aaaggacatg tagcgaaagc
    13081 taacacggct cctaagcgct tcattcgtga attcaaaaac gttgaaggct tggaagttgg
    13141 cgctgaaatc acagttgaaa cattcgcagc tggagatgtt gttgacgtaa cgggtacttc
    13201 taaaggtaaa ggtttccaag gtgttatcaa acgccacgga caatcacgtg gaccaatggc
    13261 tcacggttct cgttaccacc gtcgtccagg ttctatggga cctgttgcac ctaaccgcgt
    13321 attcaaaggt aaaaaccttg cagggcgtat gggtggcgac cgcgtaacaa ttcaaaacct
    13381 tgaagttgta caagttgttc cagaaaagaa cgttatcctt atcaaaggta acgtaccagg
    13441 tgctaagaaa tctcttatca ctatcaaatc agcagttaaa gctggtaaat aataaagaaa
    13501 ggggaaatca gtcacaatgg caaacgtaac attatttgac caaactggta aagaagctgg
    13561 ccaagttgtt cttagcgatg cagtatttgg tatcgaacca aatgaatcag ttgtgtttga
    13621 tgtaatcatc agccaacgcg caagccttcg tcaaggaaca cacgctgtta aaaaccgctc
    13681 tgcagtatca ggtggtggac gcaaaccatg gcgtcaaaaa ggaactggac gtgctcgtca
    13741 aggttctatc cgctcaccac aatggcgtgg tggtggtgtt gtcttcggac caactccacg
    13801 ttcatacggc tacaaacttc cacaaaaagt tcgtcgccta gctcttaaat cagtttactc
    13861 tgaaaaagtt gctgaaaaca aattcgtagc tgtagacgct ctttcattta cagctccaaa
    13921 aactgctgaa tttgcaaaag ttcttgcagc attgagcatc gattctaaag ttcttgttat
    13981 ccttgaagaa ggaaatgaat tcgcagctct ttcagctcgt aaccttccaa acgtgaaagt
    14041 tgcaactgct acaactgcaa gtgttcttga catcgcaaat agcgacaaac ttcttgtcac
    14101 acaagcagct atctctaaaa tcgaggaggt tcttgcataa tgaatttgta tgatgttatc
    14161 aaaaaacctg tcatcactga aagctcaatg gctcaacttg aagcagggaa atatgtattt
    14221 gaagttgaca ctcgtgcaca caaacttttg atcaagcaag ctgttgaagc tgctttcgaa
    14281 ggtgttaaag ttgccaatgt taacacaatc aacgtaaaac caaaagctaa acgtgttgga
    14341 cgttacactg gttttactaa caaaactaaa aaagctatca tcacacttac agctgattct
    14401 aaagcaatcg agttgtttgc tgctgaagct gaataatcta aggaggaaat atcgtgggaa
    14461 ttcgtgttta taaaccaaca acaaacggtc gccgtaatat gacttctttg gatttcgctg
    14521 aaatcacaac aagcactcct gaaaaatcat tgcttgttgc attgaagagc aaggctggtc
    14581 gtaacaacaa cggtcgtatc acagttcgtc accaaggtgg tggacacaaa cgtttctacc
    14641 gtttggttga cttcaaacgt aataaagaca acgttgaagc agttgttaaa acaatcgagt
    14701 acgatccaaa ccgttctgca aacatcgctc ttgtacacta cactgacggt gtgaaagcat
    14761 acatcatcgc tccaaaaggt cttgaagtag gtcaacgtat cgtttcaggt ccagaagcag
    14821 atatcaaagt cggaaacgct cttccacttg ctaacatccc agttggtact ttgattcaca
    14881 acatcgagtt gaaaccaggt cgtggtggtg aattggtacg tgctgctggt gcatctgctc
    14941 aagtattggg ttctgaaggt aaatatgttc ttgttcggct tcaatcaggt gaagttcgta
    15001 tgattcttgg aacttgccgt gctacagttg gtgttgtcgg aaacgaacaa catggacttg
    15061 taaaccttgg taaagcagga cgtagctgtt ggaaaggtat ccgcccaaca gttcgtggtt
    15121 ctgtaatgaa ccctaacgat cacccacacg gtggtggtga aggtaaagca ccagttggtc
    15181 gtaaagcacc atctactcca tggggcaaac ctgctcttgg tcttaaaact cgtaacaaga
    15241 aagcgaaatc tgacaaactt atcgttcgtc gtcgcaacga gaaataatat tagactagtc
    15301 gcttaagcaa ctagtaaatc cgccagctcg gtagcgctcc ataggagtgc aagccgctgt
    15361 ggtacaacat ttaaaggaga aaatataaaa atgggacgca gtcttaaaaa aggacctttc
    15421 gtcgatgagc atttgatgaa aaaagttgaa gctcaagcta acgacgaaaa gaaaaaagtt
    15481 attaaaactt ggtcacgtcg ttcaacgatc ttcccaagtt tcattggtta cactattgca
    15541 gtttatgacg gacgtaaaca cgtacctgtt tacatccaag aagacatggt aggccacaaa
    15601 cttggtgaat ttgcaccaac tcgtacttac aaaggtcacg ctgcagacga caagaaaaca
    15661 cgtagaaaat aaggagaaca taaatggcag aaattacttc agctaaagca atggctcgta
    15721 cagtacgtgt ttcacctcgt aaatcacgtc ttgttcttga taacatccgt ggtaaaagcg
    15781 tagccgatgc aatcgcaatc ttgacattca ctccaaacaa agctgctgaa atcatcttga
    15841 aagttttgaa ctcagctgta gctaacgctg aaaacaactt tggtttggat aaagctaact
    15901 tggtagtatc tgaagcattc gcaaacgaag gaccaactat gaaacgtttc cgtccacgtg
    15961 cgaaaggttc agcttcacca atcaacaaac gtacagctca catcactgta gctgttgcag
    16021 aaaaataagg aggtaaaatc gtgggtcaaa aagtacatcc aattggtatg cgtgtcggca
    16081 tcatccgtga ttgggatgcc aaatggtatg ctgaaaaaga atacgcggat taccttcatg
    16141 aagatcttgc aatccgtaaa ttcgttcaaa aagaacttgc tgacgcagca gtttcaacta
    16201 ttgaaatcga acgcgcagta aacaaagtta acgtttcact tcacactgct aaaccaggta
    16261 tggttatcgg taaaggtggt gctaacgttg atgcactccg tgcaaaactt aacaaattga
    16321 ctggaaaaca agtacacatc aacatcatcg aaatcaaaca acctgatttg gatgctcacc
    16381 ttgtaggtga aggaattgct cgtcaattgg agcaacgtgt tgctttccgt cgtgcacaaa
    16441 aacaagcaat ccaacgtgca atgcgtgctg gagctaaagg aatcaaaact caagtatcag
    16501 gtcgtttgaa cggtgcagat atcgcccgtg ctgaaggata ctctgaagga actgttccgc
    16561 ttcacacact tcgtgcagat atcgattacg cttgggaaga agcagatact acatacggta
    16621 aacttggtgt taaagtatgg atctaccgtg gtgaagttct tccagctcgt aaaaacacta
    16681 aaggaggtaa ataaccaatg ttagtaccta aacgtgttaa acaccgtcgt gaattccgtg
    16741 gaaaaatgcg cggtgaagca aaaggtggaa aagaagtagc attcggtgaa tacggtcttc
    16801 aagctacaac tagccactgg atcactaacc gccaaatcga agctgctcgt atcgccatga
    16861 ctcgttacat gaaacgtggt ggtaaagttt ggattaaaat cttcccacac aaatcataca
    16921 ctgctaaagc tatcggtgtg cgtatgggat ctggtaaagg ggcacctgaa ggttgggtag
    16981 caccagttaa acgtggtaaa gtgatgttcg aaatcgctgg tgtatctgaa gagattgcac
    17041 gtgaagcgct tcgacttgct agccacaaat tgccagttaa atgtaaattc gtaaaacgtg
    17101 aagcagaata aggagaaggc atgaaactta atgaagtaaa agaatttgtt aaagaacttc
    17161 gtggtctttc tcaagaagaa ctcgcgaagc gcgaaaacga attgaaaaaa gaattgtttg
    17221 aacttcgttt ccaagctgct actggtcaat tggaacaaac agttcgcttg aaagaagtta
    17281 aaaaacaaat cgctcgtatc aaaacagttc aatctgaagc gaaataatag actagggaag
    17341 gagaaatttc aatggaacgc aataatcgta aagttcttgt tggacgtgtt gtatctgaca
    17401 aaatggacaa gacaatcaca gttgtagttg aaacaaaacg taaccaccca gtctatggta
    17461 aacgtattaa ctactctaaa aaatacaaag ctcatgatga aaacaatgtt gccaaagaag
    17521 gcgatatcgt acgtatcatg gaaactcgcc cgctttcagc tacaaaacgt ttccgtcttg
    17581 tagaagttgt tgaagaagcg gtcatcatct aatcaaacct gaaaggagaa aactgaaatg
    17641 attcaaacag aaactcgttt gaaagtcgca gacaacagcg gtgctcgcga aatcttgact
    17701 atcaaagttc ttggtggttc aggacgtaaa tttgcaaaca tcggtgatgt tatcgtggca
    17761 tctgtaaaac aagctactcc tggtggtgcg gttaaaaaag gtgacgttgt taaagcagtt
    17821 atcgttcgta ctaaatcagg tgctcgtcgt gctgatggtt catacatcaa atttgacgaa
    17881 aacgcagcag ttatcatccg tgaagacaaa actcctcgcg gaacacgtat ctttggccca
    17941 gttgcacgtg aattgcgtga aggtggcttc atgaagatcg tgtcacttgc tccagaagta
    18001 ctttaatttt taggaacaaa ctagtcccct agcttcaagc tagggtgccc ttatgggcgt
    18061 aagaaaaatc aaggagaaac ctaatgtttg taaaaaaagg cgacaaagtt cgcgtaatcg
    18121 ctggtaaaga taagggaaca gaagctgttg tccttactgc ccttccaaaa gtaaacaaag
    18181 ttatcgttga aggtgttaac attgttaaga aacaccaacg tccaactaac gagcttcctc
    18241 aaggtggtat catcgagaaa gaagcagcta tccacgtatc aaacgttcaa gttttggaca
    18301 aaaatggtgt agctggtcgt gttggttaca aatttgtaga cggtaaaaaa gttcgctaca
    18361 acaaaaaatc aggcgaagtg cttgattaat cacgaaggaa aggagaagta taatggcaaa
    18421 tcgtttaaaa gaaaaatatc ttaatgaagt agttcctgct ttgacagaac aattcaacta
    18481 ctcatcagtg atggctgtgc ctaaagtaga taagattgtt ttgaacatgg gtgttggtga
    18541 agctgtatca aacgctaaaa gccttgaaaa agctgctgaa gaattggcac ttatctcagg
    18601 tcaaaaacca cttatcacta aagctaaaaa atcaatcgcc ggcttccgtc ttcgtgaagg
    18661 tgttgcgatc ggtgcaaaag ttacccttcg tggtgaacgt atgtacgaat tcttggataa
    18721 attggtatca gtttcacttc cacgtgtacg tgacttccac ggtgttccaa caaaatcatt
    18781 tgatggacgc gggaactaca cacttggtgt gaaagaacaa ttaatcttcc cagaaatcaa
    18841 cttcgatgac gttgacaaaa ctcgtggtct tgacatcgtt atcgtaacaa ctgctaacac
    18901 tgacgaagag tcacgtgcat tgcttacagg ccttggaatg ccttttgcaa aataatatag
    18961 gaggtaaatc taatggctaa aaaatcaatg gtagctagag aggctaaacg ccaaaaaatt
    19021 gttgaccgtt atgctgaaaa acgtgctgca ttaaaggcgg caggggacta cgaaggttta
    19081 tctaaattac ctcgcaacgc ctcaccgact cgtttacata atcgttgtag agttacgggg
    19141 cgcccacatt cagtttaccg caaatttggt ctgagtcgta tcgcttttcg cgaacttgcg
    19201 cataaaggtc aaattcctgg tgtaacaaaa gcatcttggt aatatcatga tacaaagagc
    19261 gtaacaacca cagcgaaaat aggaaatttg gtgagggagc gatgctccaa aacaaattta
    19321 tcttttttgc acaggttgta gctcgtgttc aaataagagt tctctcattt gaatgtgtca
    19381 atactatctg agcccagctc aatcattaac tagcaagtgc aacttgcaaa ctactagtaa
    19441 gaggagaaaa acaaaatggt tatgactgac ccaatcgcag acttcctaac tcgtattcgt
    19501 aatgctaacc aagctaaaca cgaagtactt gaagtacctg catcaaacat caaaaaaggg
    19561 attgctgaaa tccttaaacg cgaaggtttt gtaaaaaacg ttgaaatcat tgaagatgac
    19621 aaacaaggcg tcatccgtgt atttcttaaa tacggaccaa atggtgagaa agttatcact
    19681 aacttgaaac gtgtttctaa accaggactt cgtgtctaca aaaaacgtga agaccttcca
    19741 aaagttctta acggacttgg aattgccatc ctttcaactt ctgaaggttt gcttactgat
    19801 aaagaagcac gccaaaagaa tgttggtggt gaggttattg cttacgtttg gtaaaatcaa
    19861 gatacaaagc tcgtaaagaa caaagcaaaa ttaggaagtt ggagaagttt gtttacaaac
    19921 aagccaactt atctattttg cacagttctt agagcgtgtt cagttcagct cttgaactaa
    19981 ataagtatct gaaccccgtg aaaactggcc gttctggcct gacaatttaa caggagaaaa
    20041 taaacatgtc acgtattggt aataaagtta tcgtgttgcc tgctggtgtt gaactcgcta
    20101 acaatgacaa cgttgtaact gtaaaaggac ctaaaggaga acttactcgt gagttctcaa
    20161 aagatattga aatccgtgtg gatggtactg aagtaactct tcaccgtcca aacgattcaa
    20221 aagaaatgaa aactatccac ggaactactc gtgccctttt gaacaacatg gttgttggtg
    20281 tatcagaagg attcaagaaa gaacttgaaa tgcgtggggt tggttaccgt gcacagcttc
    20341 aaggatctaa acttgttttg gctgttggta aatctcatcc agacgaagtt gaagctccag
    20401 aaggaattac ttttgaactt ccaaacccaa caacaatcgt tgttagcgga atttcaaaag
    20461 aagtagttgg tcaaacagct gcttacgtac gtagccttcg ttcaccagaa ccatataaag
    20521 gtaaaggtat ccgttacgtt ggtgaattcg ttcgccgtaa agaaggtaaa acaggtaaat
    20581 aatgttgagt ggttgatcat caaccaccaa cctattttcc aactttgtgc atagcacacg
    20641 atttaaaact aaagaggtga aaactgtgat ttcaaaacca gataaaaaca aactccgcca
    20701 aaaacgccac cgtcgcgttc gcggaaaact ctctggaact gctgatcgcc cacgtttgaa
    20761 cgtattccgt tctaatacag gcatctacgc tcaagtgatt gatgacgtag cgggtgtaac
    20821 gctcgcaagt gcttcaactc ttgataaaga agtttcaaaa ggaactaaaa ctgaacaagc
    20881 cgttgctgtc ggtaaactcg ttgcagaacg tgcaaacgct aaaggtattt cagaagtggt
    20941 gttcgaccgc ggtggatatc tatatcacgg acgtgtgaaa gctttggctg atgcagctcg
    21001 tgaaaacgga ttgaaattct aataggagga cactagaaaa tggcatttaa agacaatgca
    21061 gttgaattag aagaacgcgt agttgctgtc aaccgtgtta caaaagttgt taaaggtgga
    21121 cgtcgtcttc gtttcgcagc tcttgttgtt gttggtgacc acaatggtcg cgtaggattt
    21181 ggtactggta aagctcaaga agttccagaa gcaatccgta aagcagtaga tgatgctaag
    21241 aaaaacttga tcgaagttcc tatggttgga acaacaatcc cacacgaagt tctttcagaa
    21301 ttcggtggag ctaaagtatt gttgaaacct gctgtagaag gttctggagt tgccgctggt
    21361 ggtgcagttc gtgccgttgt ggaattggca ggtgtggcag atattacatc taaatcactt
    21421 ggttctaaca ctccaatcaa cattgttcgt gcaactgttg aaggtttgaa acaattgaaa
    21481 cgcgctgaag aaattgctgc ccttcgtggt atttcagttt ctgatttggc ataagaaagg
    21541 ggataaaatg gctcaaatta aaattacttt gactaagtct ccaatcggac gcattccatc
    21601 acaacgtaaa actgttgtag cacttggact tggcaaattg aacagctctg ttattaaaga
    21661 agataacgct gctatccgtg gtatgatcac agcagtatct cacttagtaa cagttgaaga
    21721 agtaaactag atatagtcat ctatgatgac atcgtatagg cgagttgatg ggggagacaa
    21781 ccttttctcc cttatcggcg ctagcatttt acaaaagagg agaaaataaa aatgaaactt
    21841 catgaattga aacctgcaga aggttctcgt aaagtacgta accgcgttgg tcgtggtact
    21901 tcatcaggta acggtaaaac atctggtcgt ggtcaaaaag gtcaaaaagc tcgtagcggt
    21961 ggcggagttc gccttggttt tgaaggtgga caaactccat tgttccgtcg tcttccaaaa
    22021 cgtggattca ctaacatcaa cgctaaagaa tacgcaattg tgaaccttga ccaattgaac
    22081 gtctttgaag atggtgctga agtaactcca gttgttctta tcgaagcagg aattgttaaa
    22141 gctgaaaagt caggtattaa aattcttggt aacggtgagt tgactaagaa attgactgtg
    22201 aaagcagcta aattctctaa atcagctgaa gaagctatca ctgctaaagg tggttcagta
    22261 gaagtcatct aagagaggtg acctatgttt tttaaattat taagagaagc tcttaaagtc
    22321 aagcaggttc gatcaaaaat tttatttaca atttttatcg ttttggtctt tcgtatcgga
    22381 actagcatta cagttcctgg tgtgaatgcc aatagcttga atgctttaag tggattatcc
    22441 ttcttaaaca tgttgagctt ggtgtcgggg aatgccctaa aaaacttttc gatttttgcc
    22501 ctaggagtta gtccctatat caccgcttct attgttgtcc aactcttgca aatggatatt
    22561 ttacccaagt ttgtagagtg gggtaaacaa ggggaagtag gtcgaagaaa attgaatcaa
    22621 gctactcgtt atattgctct agttctcgct tttgtgcaat ctatcgggat tacagctggt
    22681 tttaatacct tggctggagc tcaattgatt aaaactgctt taactccaca agtttttctg
    22741 acgattggta tcatcttaac agctggtagt atgattgtca cttggttggg tgagcaaatt
    22801 acagataagg gatacggaaa cggtgtttcc atgattatct ttgccgggat tgtttcctca
    22861 attccagaga tgattcaggg catctatgtg gactactttg tgaacgtccc aagtagccgt
    22921 atcacttcat ctatcatttt cgtaatcatt ttgattatta ctgtattgtt gattatttac
    22981 tttacaactt atgttcaaca agcagaatac aaaattccga tccaatatac taaggttgca
    23041 caaggtgctc catctagctc ttaccttccg ttaaaggtaa atcctgctgg agttatccct
    23101 gttatctttg ccagttcgat tactgcagcg cctgcggcta ttcttcagtt tttgagtgcc
    23161 acaggtcatg attgggcttg ggtaagggta gcacaagaga tgttggcaac tacttctcca
    23221 actggtattg ccatgtatgc tttgttgatt attctcttta cattcttcta tacgtttgta
    23281 cagattaatc ctgaaaaagc agcagagagc ctacaaaaga gtggtgccta tatccatgga
    23341 gttcgtcctg gtaaaggtac agaagaatat atgtctaaac ttcttcgtcg tcttgcaact
    23401 gttggttccc tcttccttgg tgtgatttcc attttaccga ttgcagctaa agatgtattt
    23461 ggtctttctg atgttgttgc ctttggtgga acaagtctct tgatcattat ctctacaggt
    23521 atcgaaggaa tcaagcaatt ggaaggttac ctattgaaac gtaagtatgt tggtttcatg
    23581 gacaaaacag aataaaagta tttactgaat cagtaaatac tgagggagtg gaggtttaaa
    23641 ctctgacatt tgtaagagtt ggatctcccc tcttctattt tgtttttaaa taggggtgaa
    23701 aagacttttt gcttctattt aaaaataaaa taaggagatc aaatcatgaa tcttttgatt
    23761 atgggcttac ctggtgcagg taagggaact caagcagcaa aaatcgtaga acaattccat
    23821 gttgcacata tctcaacagg tgatatgttc cgcgctgcaa tggtaaatca aactgaaatg
    23881 ggtgttcttg ctaagtcata tattgacaag ggtgaattgg ttcctgacga agttacaaat
    23941 ggaatcgtaa aagaacgtct ttcacaagat gatattaaag aaacaggatt cttattggat
    24001 ggttacccac gtacaattga acaagctcat gccttggaca aaacattggc tgaacttggc
    24061 attgaactag aaggtgttat caatattgaa gtgaaccctg acagcctctt ggaacgtttg
    24121 agtggccgta tcatccaccg cgtaactgga gaaactttcc acaaggtctt taacccacca
    24181 gttgactata aagaagaaga ctactaccaa cgtgaagatg ataagcctga gacagtaaaa
    24241 cgtcgtttgg atgttaatat tgctcaagga gaaccaatca ttgctcacta ccgtgccaaa
    24301 ggtttggttc atgacatcga aggtaatcaa gatatcaatg atgtcttctc agatattgaa
    24361 aaagtattga caaatttgaa ataaagcgtt tttcacactt gcaaaaatcc gctacaaatg
    24421 ttatactgag atagtctgac ttataattgt tgtctctgtg tctagaggca tcgaatcgaa
    24481 atttatggag gtgcttttgc gtggcaaaag acgatgtgat tgaagttgaa ggcaaagtag
    24541 ttgatacaat gccgaatgca atgtttacgg ttgaacttga aaatggacat cagattttag
    24601 caacagtttc tggtaaaatt cgtaaaaact atattcgtat tttagcggga gatcgtgtta
    24661 ctgtcgaaat gagtccatat gacttgacac gtggacgtat cacttaccgc tttaaataat
    24721 cgaaaaactt ggagggataa gaaatgaaag taagaccatc ggtcaaacca atttgcgaat
    24781 actgtaaagt tattcgtcgt aatggtcgtg ttatggtaat ttgcccagca aatccaaaac
    24841 acaaacaacg tcaaggataa gatagaaagg agaaaacatg gctcgtattg ctggagttga
    24901 tattccaaat gacaaacgcg tagtaatctc attgacttat gtttatggta tcggacttgc
    24961 aacatctaag aagattttgg ctgctgctgg aatctcagaa gatgttcgtg tacgtgatct
    25021 tacatcagat caagaagatg ctatccgtcg tgaagtggat gcaatcaaag ttgaaggtga
    25081 ccttcgtcgt gaagtaaact tgaacatcaa acgtttgatg gaaatcggtt cataccgtgg
    25141 tatccgtcac cgtcgtggac ttcctgtccg tggacaaaat actaaaaaca acgctcgcac
    25201 tcgtaaaggt aaagctgttg cgattgctgg taagaaaaaa taatatagga ggtaaaagtc
    25261 ttggctaaac caacacgtaa acgtcgtgtg aaaaagaata tcgaatctgg tattgctcat
    25321 attcacgcta catttaataa cactattgtt atgattactg atgtgcatgg taatgcaatt
    25381 gcttggtcat cagctggtgc tcttggtttc aaaggttctc gtaaatctac accattcgct
    25441 gctcaaatgg cttctgaagc tgctgctaaa tctgcacaag aacacggtct taaatcagtt
    25501 gaagttactg taaaaggtcc aggttctggt cgtgagtcag ctattcgtgc gcttgctgcc
    25561 gctggtcttg aagtaacagc aattcgtgat gtgactccag tgccacacaa tggtgctcgt
    25621 cctccaaaac gtcgccgtgt ataatcatcg cattacactg cttttcgttt aagagggagt
    25681 aactaaatga tcgagtttga aaaaccaaat ataacaaaaa ttgatgaaaa taaagattat
    25741 ggcaagtttg taatcgaacc acttgaacgt ggctacggta caactcttgg taactctctt
    25801 cgtcgtgtac ttctagcttc tctaccagga gcagctgtga catctatcaa cattgatggt
    25861 gtgttacatg agtttgacac agttccaggt gttcgtgaag acgtgatgca aatcattctg
    25921 aacattaaag gaattgcagt gaaatcgtac gttgaagacg aaaaaatcat cgaactggat
    25981 gttgaaggtc ctgctgaagt aacagctggt gacattttga cagatagcga tattgaaatt
    26041 gtaaatccag atcattatct ctttacaatc ggtgaaggtt cttctctaaa agcgactatg
    26101 actgttaaca gtggtcgtgg atatgtacct gctgatgaaa ataaaaagga taatgcacca
    26161 gttggaacac ttgctgtaga ttctatttat acaccagtta caaaagtcaa ctatcaagtg
    26221 gaacctgctc gtgtaggtag caatgatggt ttcgacaaat taacccttga aatcttgaca
    26281 aatggaacaa ttattccaga agatgcttta gggctttcag cacgtatttt gacagaacat
    26341 cttgatttgt ttacaaatct tactgagatt gctaagtcaa ctgaagtgat gaaagaagct
    26401 gatactgaat ctgacgaccg tattttagat cgtacgattg aggaactgga cttgtctgtg
    26461 cgttcataca actgtttaaa acgtgccggt atcaatactg tgcatgattt gacagaaaaa
    26521 tctgaagcag agatgatgaa agtacgaaat cttggacgca agagtttgga agaagtgaaa
    26581 ctcaaactca ttgatttggg tcttggatta aaagataaat aaaggaggaa tacatggctt
    26641 accgtaaact aggacgcact agctcacaac gtaaagcaat gcttcgcgat ttgacaactg
    26701 accttttgat caacgaatca atcgtgacaa ctgaagctcg tgctaaagaa atccgtaaaa
    26761 ctgttgaaaa aatgattact ctaggtaaac gtggtgattt gcatgcacgt cgtcaagcag
    26821 ctgctttcgt acgtaatgaa atcgcatctg aaaactatga tgaagcaact gataagtaca
    26881 cttctactac agcacttcaa aaattgttct cagaaatcgc acctcgttat gctgaacgta
    26941 acggtggata cactcgtatc cttaaaactg aaccacgtcg tggtgatgca gcgccaatgg
    27001 cgatcatcga attagtataa aatcatcaat tttgttgagt gttatgatga tggagtcttg
    27061 tgctcttagt ctagctctgg tctaccgcta ggatttcggt cctagcggga acactcatca
    27121 taagttggga tagtagacgc ttgtttacga aattgttttt ttcttaagaa caacttcgta
    27181 agcaggcgtt tttgagtatt ttcgttagaa ttatgctata ctatttgaaa agaatcctgt
    27241 ttaatgttaa ggtttcttat tttaagaaga attggagttt acttatgaaa gccattataa
    27301 ctgttgttgg taaagataaa tctggaattg ttgcaggtgt ttctggtaaa attgcagaat
    27361 taggattgaa tattgacgat atctctcaaa ctgtcttgga tgaatatttt acgatgatgg
    27421 ctgttgtatc tagtgatgaa aagcaagatt ttacctatct tcgtaatgaa tttgaagctt
    27481 ttgggcaaac tttgaatgta aaaatcaata ttcagagtgc agcgattttc gaagctatgt
    27541 ataatatcta ggaggtcatc atggatatta gacaagttac tgaaaccatc gccatgattg
    27601 aggagcaaaa cttcgatatt agaaccatta ccatggggat ttctcttttg gactgtatcg
    27661 atccagatat caatcgtgct gcggagaaaa tctatcaaaa aattacgaca aaggcggcta
    27721 atttagtagc tgttggtgat gaaattgcgg ctgagttggg aattcctatc gttaataagc
    27781 gtgtatcggt gacacctatt tctctgattg gggcagcgac agatgcgacg gactacgtgg
    27841 ttctggcaaa agcgcttgat aaggctgcga aagagattgg tgtggacttt attggtggtt
    27901 tttctgccgt agtacaaaaa ggttatcaaa agggagatga gattctcatc aattccattc
    27961 ctcgcgcttt gactgagacg gataaggtct gctcgtcagt caatatcggc tcaaccaagt
    28021 ctggtattaa tatgacggct gtggcagata tgggacgaat tatcaaggaa acggcaaatc
    28081 tttcagatat gggagcggcc aagttggttg tattcgctaa tgctgttgag gacaatccat
    28141 ttatggcggg tgcctttcat ggtgttgggg aagcagatgt tatcatcaat gtcggagttt
    28201 ctggtcctgg tgtggtgaaa cgtgctttgg aaaaagttcg tggacagagc tttgatgtag
    28261 tagccgaaac agttaagaaa actgccttta aaatcactcg tatcggtcaa ttggttggtc
    28321 aaatggccag tgagagactg ggtgtggagt ttggtattgt ggacttgagt ttggcaccaa
    28381 cccctgcggt tggagactct gtggcacgtg tccttgagga aatggggcta gaaacagttg
    28441 gcacgcatgg aacgacggct gccttggccc tcttgaacga ccaagttaaa aagggtggag
    28501 tgatggcctg caaccaagtc ggtggtttat ctggtgcctt tatccctgtt tctgaggatg
    28561 aaggaatgat tgctgcagtg caaaatggct ctcttaattt agaaaaacta gaagctatga
    28621 cggctatctg ttctgttgga ttggatatga ttgccatccc agaagatacg cctgctgaaa
    28681 ccattgcggc tatgattgcg gatgaagcag caatcggtgt tatcaacatg aaaacaacag
    28741 ctgttcgtat cattcccaaa ggaaaagaag gcgatatgat tgagtttggt ggtctattag
    28801 gaactgcacc cgttatgagg gttaatgggg cttcgtctgt cgacttcatc tctcgcggtg
    28861 gacaaatccc agcaccaatt catagtttta aaaattaaga aaataggaga aattttaagt
    28921 tctatttaag attagacgtg tatactataa tcattaaata aagacctcct aatattattt
    28981 gaaacagata acattgaatt agtttgaatt tgattttcat ctaatatctt tatttaatga
    29041 actcctaaac tttttcataa taatctcctt caaaagtcgc ctgtatgggt ggcttttatt
    29101 ttatcattca tgatataata gaagcaaacg gaggacggaa aatggtaaaa gtacgattgt
    29161 atttggtacg tcatggcaag accatgttta acacgattgg tcgcgcgcaa ggttggagcg
    29221 atactccctt aactgctgaa ggtgaacgag ggattcaaga gttaggaatc ggtttgcgag
    29281 aatctgatct acagtttgag cgcgcttatt cgagtgattc tggtcgtacc attcagacca
    29341 tgggaattat ccttgaagag cttggcttgc aggggaaaat cccttatcgc atggacaagc
    29401 gtatcagaga atggtgtttc ggtagttttg atggagccta tgatggcgat cttttcatgg
    29461 gcattattcc tcgtatcttt aatgtggacc acgttcacca attgtcttat gctgaactgg
    29521 ctgagggctt ggtagaggtc gatacagctg gttgggctga aggctgggaa aaactcagtg
    29581 gccgaatcaa ggaaggcttt gaaatgattg caaaagaaat ggaagatcaa ggtggaggta
    29641 acgcccttgt tgtcagccat ggaatgacta ttggaaccat tgtttatctg attaatggca
    29701 tgcatccgca tggtctagat aatggtagcg tgacgattct tgaatatgag ggcggtcagt
    29761 ttagggttga agttgtcggt gaccgtagtt accgagagct aggacgtgag aagatggaag
    29821 aaggctctat ttaatcagtc tagacttgct tgccatgagc tagggatttg ataagaatat
    29881 caagataaga aaaaacagcc gagggcactc ctttcggctg tttttgatgt ggaaaactaa
    29941 agggtaaagc tattgctttt agagattttc ataaacaaga gcaaggaacc tactgttaga
    30001 acagtcagga tagttgacaa ggctgcggct acaccgtaat ttcctctgag aacctctgta
    30061 taaatagcta cagtcattgt tcttgttttg acattgtaga ggaggataga agtagagagt
    30121 tttgaaatca ttgtgaccca agataagatg actccagaaa taatacccga tagcatcatt
    30181 ggagttgtaa tcttagcaaa gatattgaga cgactacttc ctaagctttc agcagcttct
    30241 tcaatacttg gtgctatttg ttgtaagcta gcaacagatg agcgaatagt atacggtaat
    30301 cttctgacag atagagacat aatcaagatg aaagcagtcc ctgtaatcat aagaaatcca
    30361 cttccaaata gaccagtatt gaaggaagaa atgaaggcaa tccctagaac ggttcctggt
    30421 acaatataag gtaccatact gaggctgtca attaagtttg taaacaaatt ccgttttcta
    30481 acggctaggt aggagataaa tgtcgcaaat agaacaacta gaactaaggc aatcaaaggg
    30541 atacgaatgg tattgaaaat agcagatccc atacgattga aagctacctt gtaactgttt
    30601 ggagaataac ctttaacaaa taccatacct gatgttttta ggaaagaggt ataaattaag
    30661 tagatttgag gtaaaacaga gataaagata attccgtaga ctgttgcata aatggcagcc
    30721 atttttcctt ttgtagtttt tttaggctca attggatgga gcagattcat gctgaaactg
    30781 tagcggtttg caatgtgttt ttggataagg aaaattgcca aggcaatgat aatcgccata
    30841 attgcaaaag cagaatttcc tccaacctcg ctaataaatt gggtataaat caggacaggg
    30901 aaagtccgat atccttcgcc aatcaacata ggcgttccaa agtctgagaa tgctctcata
    30961 aatacaagca aggagctgct agtaaggttg gaactaggag aggtaaaaca accgttacga
    31021 taggtttaaa tccgaaggac cccatgcttt cagctgcttc aagtagagaa ttgtcaatac
    31081 tgttcattgt tccagcaaca tatagaaata ccagtgggaa tagttgcagt gtaaagacaa
    31141 gtacaattcc tttgaatcca taaatatcga tagctggaag atgaagggca tttgtcaaaa
    31201 atttagtgat gaccccattt cgtcccagca agagaaccca ggagtaggct cctacgaaag
    31261 gagctgacat ggaagcaatg ataatcaata tttgtagaaa tttcttcccc ttgaagtcat
    31321 acatagagaa gagataagct aatagggttc ctacaactaa ggaagtgaca gtagcggtaa
    31381 tggaaaccct gaaactgttg actagtgtct cagagtagta ggctttacta aagaaagtga
    31441 caaaattagc tagtgagaat tgtccttcat gtataagtgc ttgcttgagc acggtaacga
    31501 taggataaac gagaaagaca agataggtaa gaaagatgaa gaaagaggaa gctgtccaaa
    31561 tatttagttt tttacattcc atggttgact ccttttatca ggttttggga accatctgca
    31621 gaaaagatgt ttaatttttg cgtattgatt cgtagacgaa tacgattgcc tttttgtagt
    31681 tcttcttcaa aagttgattc ttcactaact taaatttttg aggcaaaacc tgtctcaatg
    31741 aaatactccg tatttagtcc aagatagacg ctatcgctaa tagttccttc gatatctcca
    31801 gatttatctt tgataaactc ttcgggacga atgcttacat gaatagcttg ctcctcaacc
    31861 tgatcaagag ctggcattcg aagggcatag ccatctgaaa agacgatata agcgccgtcg
    31921 ctccgttttt caagattggc agggataata tttgtgcgtc cgataaaggt tgccacaaac
    31981 tcattagctg gtttatgata gagttatttt ggtcagccga tttgttggat caccccatct
    32041 ttcataacag caatttggtc tgaaatagcc atggcttctt cttggtcgtg ggttacataa
    32101 acagttgtaa ttcccacttc gtgttggatt tctcggatgg cttgacgcat atccaagcga
    32161 agtttggcct ccagattact aagtggctcg tccatgagga gaacacttgg attaaccgct
    32221 aaggcgcatg ccaaggtgac acgttgttgt tgtccaccac tgagtttatc gggctttcga
    32281 tccgcatatt gagcaatttg catgagttca agatacttgt tggtctgttg aatcaattct
    32341 tcttttggaa ccttcttttg cttaagacca aaagcaacgt tgtctcggac agtcaaatgt
    32401 gggaaaatag cgtagttttg gaaaaccatc ccgatattgc gtttgctgga ttccatatta
    32461 ttgatttttg tatcatcgaa gtattgatac tgttgaaacc tgcaatcata cgaagaaggg
    32521 tcgttttccc gcatcctgaa gctccaagaa gggtaaagag acttcctttt ggaattgtaa
    32581 tgttcaaatt ctcaataaca gggacatcgt ggtagatttt tttggcgtta ataatttcga
    32641 tctcactcat agtgaacctc ttttactgtt tagattggat atctgtaaag acttcgttgt
    32701 atttcttaac gatatctgat ttattcttga tgacataatc ataatcttca gtgagtgttt
    32761 tgattttgtc aattggtttc atgttttcgc ttgttttagc atttttacga acaggacggt
    32821 tagtagtggt tgtaccaagt gtatcttgta cttcttgaga gataataaaa tcgataaatt
    32881 tcttggcagt ttccatattt ttagattttt taacgatagc agcactagca ggtaggaaga
    32941 cggttccttc ttttggatag actaccttaa tgttagctcc gtcatttaag agtttaactg
    33001 ctggatcttc ataagagaga ccaacagtca tttctacatc agcgactgct ttatagacac
    33061 cagatgaact taaaccgatt ttatagtata ttgaaactag aatagtacac atctgcttct
    33121 aaaacattgt tagaaatcga tttgactgtc ctgattaatt tgtcctattc ttatttcatt
    33181 ttactatacc atcaataagt gtgaaaagat cttttacata agaccaagcc ttatcatctt
    33241 tgtaaccacc ttgagcttgt agcatatttg ttaattgagc aaaggcgcta gaagagtttg
    33301 ctgggtcagc agttgcgatt tttcctttta gttcaggttt gagaaggtcg ctatatcctt
    33361 cgatgttcat gcctttagtt aaatcagggt ggacgattaa aacactacca tctagtgtat
    33421 aaggagtaga gaagccagtt gtgttttgat attctttgat aacattatca ttttcttttg
    33481 aaatatagtt ttcaaagagt tctccgtggg tagcatattg tgtataagaa ccaccaaaga
    33541 taacatcagc tacaggaact tctttttctg actctagttt tttgaaaagt tctccagtac
    33601 cagcttgaat cagttctatt ttgataccat atttttcttc aaaggcagga atagttgctc
    33661 caattaagcc ttctgagttt ggtgaataaa cgactagcga accaccgtct cctttatcag
    33721 atgaactgtc atcggcagat tcattagaag aacaagcagc ataatacatc catttctttt
    33781 tcatgatgga tacctccgtt gtgttattta agtttatttt aaaacaatgt aagcgttttt
    33841 aaaacctaca attcta
//
LOCUS       DAVKDZ010000017        32402 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000017 DAVKDZ010000000
VERSION     DAVKDZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32402
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.017"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..331
                     /locus_tag="VWZ30_000780"
     CDS             <1..331
                     /locus_tag="VWZ30_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334558.1"
                     /translation="RIVKEKIKYDKIINATQEKYREEKKKLLSTLRKEYYSIPTEFRT
                     YNHISGIQYFLNADIASNLQQAIHYYSNEYNSKRMQDQIYSLHHSNTILQDRIKELEL
                     ERELEKY"
     gene            352..744
                     /locus_tag="VWZ30_000781"
     CDS             352..744
                     /locus_tag="VWZ30_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334559.1"
                     /translation="MTKFNLQSNQIYSELSKFEIEEILYNATKQLNGSVERQSSEEIS
                     YSGQGNFFDYMKHPGLSRYTCWQVLVTISDLGDKRKVELTAVGESPISYLLNNFLSFI
                     TFYTLFQRHRNLNFGISKEKAKYLSNLL"
     gene            complement(930..2492)
                     /gene="guaA"
                     /locus_tag="VWZ30_000782"
     CDS             complement(930..2492)
                     /gene="guaA"
                     /locus_tag="VWZ30_000782"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU9334560.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREINPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPSDFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIQK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRVLGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            2634..3332
                     /locus_tag="VWZ30_000783"
     CDS             2634..3332
                     /locus_tag="VWZ30_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9334561.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(3375..4271)
                     /locus_tag="VWZ30_000784"
     CDS             complement(3375..4271)
                     /locus_tag="VWZ30_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9334562.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMILAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLIPLKDLFLEK"
     gene            complement(4280..5215)
                     /locus_tag="VWZ30_000785"
     CDS             complement(4280..5215)
                     /locus_tag="VWZ30_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEU9334563.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKVGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(5212..5937)
                     /locus_tag="VWZ30_000786"
     CDS             complement(5212..5937)
                     /locus_tag="VWZ30_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEU9334564.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLVSISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLCKDRNNVELWFEEV"
     gene            complement(6021..6656)
                     /locus_tag="VWZ30_000787"
     CDS             complement(6021..6656)
                     /locus_tag="VWZ30_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEU9334565.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLHGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEKYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQMEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(6658..7485)
                     /locus_tag="VWZ30_000788"
     CDS             complement(6658..7485)
                     /locus_tag="VWZ30_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9334566.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAKTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYKRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(7507..8564)
                     /locus_tag="VWZ30_000789"
                     /pseudo
     CDS             complement(7507..8564)
                     /locus_tag="VWZ30_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813133.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            complement(8707..8898)
                     /locus_tag="VWZ30_000790"
     CDS             complement(8707..8898)
                     /locus_tag="VWZ30_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334567.1"
                     /translation="MKEILICDIIKRKGLHKKVGRMKMQRRQNRNQGGLAFRFMKGLV
                     NFLGVIAIGAIRDLWRYSC"
     gene            9087..9698
                     /locus_tag="VWZ30_000791"
     CDS             9087..9698
                     /locus_tag="VWZ30_000791"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9334568.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9744..10472
                     /gene="rlmB"
                     /locus_tag="VWZ30_000792"
     CDS             9744..10472
                     /gene="rlmB"
                     /locus_tag="VWZ30_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU9334569.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNLEKVKELAT
                     EKKVSISWISKKSLSEMTEGPVHQGFVLRVSEFAYSELDCILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(10511..11422)
                     /gene="trxB"
                     /locus_tag="VWZ30_000793"
     CDS             complement(10511..11422)
                     /gene="trxB"
                     /locus_tag="VWZ30_000793"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU9334570.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(11488..11712)
                     /locus_tag="VWZ30_000794"
     CDS             complement(11488..11712)
                     /locus_tag="VWZ30_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEU9334571.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(11790..12533)
                     /locus_tag="VWZ30_000795"
     CDS             complement(11790..12533)
                     /locus_tag="VWZ30_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9334572.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(12533..13333)
                     /locus_tag="VWZ30_000796"
     CDS             complement(12533..13333)
                     /locus_tag="VWZ30_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9334573.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMDISAPDTVQTDVKGDLR"
     gene            complement(13491..13847)
                     /locus_tag="VWZ30_000797"
     CDS             complement(13491..13847)
                     /locus_tag="VWZ30_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9334574.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNREAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13841..14371)
                     /locus_tag="VWZ30_000798"
     CDS             complement(13841..14371)
                     /locus_tag="VWZ30_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU9334575.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGEPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRNNRRRSTC"
     gene            complement(14371..14865)
                     /locus_tag="VWZ30_000799"
     CDS             complement(14371..14865)
                     /locus_tag="VWZ30_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9334576.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            15000..15446
                     /locus_tag="VWZ30_000800"
     CDS             15000..15446
                     /locus_tag="VWZ30_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEU9334577.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYINQVCTPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            15443..16090
                     /locus_tag="VWZ30_000801"
     CDS             15443..16090
                     /locus_tag="VWZ30_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU9334578.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMITGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(16111..16692)
                     /gene="pdxT"
                     /locus_tag="VWZ30_000802"
     CDS             complement(16111..16692)
                     /gene="pdxT"
                     /locus_tag="VWZ30_000802"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU9334579.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(16693..17568)
                     /gene="pdxS"
                     /locus_tag="VWZ30_000803"
     CDS             complement(16693..17568)
                     /gene="pdxS"
                     /locus_tag="VWZ30_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU9334580.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLRKDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(17720..19099)
                     /locus_tag="VWZ30_000804"
     CDS             complement(17720..19099)
                     /locus_tag="VWZ30_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU9334581.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKDKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            19394..20317
                     /locus_tag="VWZ30_000805"
     CDS             19394..20317
                     /locus_tag="VWZ30_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU9334582.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGISPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            20377..20982
                     /locus_tag="VWZ30_000806"
     CDS             20377..20982
                     /locus_tag="VWZ30_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9334583.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDVIFDDFRIFVKITEKLRNAQELLRKDAEEFNWENL"
     gene            21000..22244
                     /locus_tag="VWZ30_000807"
     CDS             21000..22244
                     /locus_tag="VWZ30_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9334584.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRNFKFKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(22361..22618)
                     /locus_tag="VWZ30_000808"
     CDS             complement(22361..22618)
                     /locus_tag="VWZ30_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEU9334585.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRVV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(22660..24696)
                     /locus_tag="VWZ30_000809"
     CDS             complement(22660..24696)
                     /locus_tag="VWZ30_000809"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU9334586.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGGRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKKIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYALLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTLKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(24956..25873)
                     /gene="glyQ"
                     /locus_tag="VWZ30_000810"
     CDS             complement(24956..25873)
                     /gene="glyQ"
                     /locus_tag="VWZ30_000810"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU9334587.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQCCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            complement(26066..26827)
                     /locus_tag="VWZ30_000811"
     CDS             complement(26066..26827)
                     /locus_tag="VWZ30_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9334588.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QPVIWSDFNAATQIIRESAVPTYTNNRRVVMTPDLAVWKELYLYQLMDYECSEQTQAI
                     ESHYHSLSENFLLHIVGHELAHWSEHFLDDFDGSDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTDKTLPLLNWFVQQKLIEKEI"
     gene            complement(27100..27942)
                     /locus_tag="VWZ30_000812"
     CDS             complement(27100..27942)
                     /locus_tag="VWZ30_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU9334589.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEEVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTTAVQSGAPRVD
                     DVDF"
     gene            complement(28056..29447)
                     /locus_tag="VWZ30_000813"
     CDS             complement(28056..29447)
                     /locus_tag="VWZ30_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU9334590.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYGIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(29682..30059)
                     /locus_tag="VWZ30_000814"
                     /pseudo
     CDS             complement(29682..30059)
                     /locus_tag="VWZ30_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            30292..31269
                     /locus_tag="VWZ30_000815"
     CDS             30292..31269
                     /locus_tag="VWZ30_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9334591.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGKQVQLPIQQAPHT
                     MTEEVAAFSHMIQQPDLNLYQTWLDDAGSVHELLYTMRQTAGIRFEAEK"
     gene            31266..32348
                     /locus_tag="VWZ30_000816"
     CDS             31266..32348
                     /locus_tag="VWZ30_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9334592.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGSEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLNNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
BASE COUNT         9697 a         7226 c         5796 g         9683 t
ORIGIN      
        1 cagaattgtt aaagaaaaaa taaaatacga taaaataata aatgcgactc aagaaaaata
       61 tagggaagaa aagaaaaaat tattatctac tttgcgaaaa gagtactact ccattccaac
      121 tgaatttaga acatataatc atatttcagg tatacaatat tttttaaacg ctgatattgc
      181 atcaaatttg caacaagcta ttcactatta ttcaaatgaa tacaattcta aaagaatgca
      241 agaccaaatt tatagcttac atcattcaaa cacaatatta caagatagaa ttaaggagct
      301 cgaattagaa agagagctgg aaaaatacta atcaaaaagg agaatactac aatgacaaaa
      361 tttaatttac aatcaaatca aatttatagt gaattaagta agtttgagat agaagaaata
      421 ctgtataatg ctacaaaaca attgaacggt agtgttgaac ggcaaagttc tgaagaaatt
      481 tcatactctg gacagggaaa tttttttgat tacatgaagc acccaggttt aagtagatat
      541 acatgttggc aagtcttagt tactatatca gatttagggg ataaacgaaa agttgagcta
      601 actgcagtcg gagaaagtcc gattagttac ctactaaata attttctatc attcattact
      661 ttttatacct tattccaacg tcatcgtaat cttaattttg gcatatcaaa agaaaaggct
      721 aaataccttt ctaatttgtt gtagaagata atttatatac aaaaaactct cttagtacat
      781 attcacacta agagagtttt ttgtaatcta tttgccaagt ttggtgggaa aattggcggg
      841 gtaacttttc taaaaagata aatttatacc attctaactt ctataaaccc gatagaatcg
      901 gtgtaaaaca ttcattttta ctttctatat tattcccact caactgttgc aggtggttta
      961 cttgtaatat cgtagacgat acggttaaca tgatccactt catttacgat acgtacagag
     1021 attttttgaa ggacttccca aggaattttg gcaaagtcag ctgtcatacc atcgatagaa
     1081 gtgatagcac ggattgcaat cgtgtagtca tacgtacgac cgtcacccat aacaccaact
     1141 gaacgaacgc ctgtgttaac agtgaagtat tgccagatat cacggtcaag acctgcttta
     1201 gcaatttctt cacgaagaat agcatctgac tcacgaactg tttcaagttt ttcttcagtt
     1261 atttcaccca tgacacggat cgcaagacct ggtcctggga atggttggcg ccatacgata
     1321 tggtctggca taccaagctc tgtaccaagg acacgaactt cgtctttata aagagtattg
     1381 agtggttcaa tcaattcaaa ctgcatgtct tctggaagac cacccacgtt gtggtgtgac
     1441 ttgatagttt gagctgtatc cgtaccagac tcgatcacgt cggtatataa ggtaccttga
     1501 gcaaggaatt tcacatcttt gagcttgctt gcttcgtcat caaatacata gacaaactcg
     1561 ttaccgataa ttttacgttt ttgttcaggg tcagaaacac ctgcaagttt atcaaggaaa
     1621 cgcttagctg catctgcttt gacgatattc aaaccaaact taccaccaag catgtccata
     1681 acttggtctg cttccccttt acgaagaaga ccgtggtcaa cgaagataca gattaattga
     1741 tctccaattg ctttttggag aagaactcca acaactgagg agtcaacacc gcctgataga
     1801 ccaagaagga cacgtttatc accgacggtt tcacgaattt tttggatctg catatcgatg
     1861 aagttatcca ttgaccagtc gcctttagct ttacagatgt taagggcaaa gttacgaaga
     1921 atatcatttc cgtatacaga atgacgaact tctggatgga attggatacc gtaaatatgt
     1981 ttatcagggt tttcaatggc tgcgtatggg cagtcagcgg atgtacctgt acgaacaaag
     2041 tcagaaggaa tttcagtaac tgcatcacca tggctcatca atacagtttg ttcatcaggt
     2101 gttgattcaa aaagagctga tggtgtatgt gttagggttg attgaccgta ttcacgattt
     2161 ccagcatcac ctgcaggaac aacttttcct ccaagcttat gggtcaataa ctgcataccg
     2221 taacaaattc ccaaaatagg aattccgagt tcgaagattt ctgggtcaat atcaaatgaa
     2281 ccatcttcat atacagaatt tggaccacct gaaaggatga ttcctacagg attgatttca
     2341 cgaacttcag cagctgaaat tttatggctt tttagttctg aaaaaacacc aatctcacgg
     2401 atacggcgtg aaatcagctg gttgtactgg ctaccatagt ccaatacgat gattttttct
     2461 acatcttgca aatcagttga aatgttgctc atctttttcc tttctcaaaa gaaatatctt
     2521 ttctcaatat tctatcacaa ataagggcga attaccaact aaacaaggcg aagtttgact
     2581 ttttctagtt tattggggta caatagagaa aagatagaaa tgaagtgaaa aatatgctac
     2641 ctgcttatat gaaaatccat gatcagataa aaaaggatat tgacgagcac cgttgggcta
     2701 ttggtgagag gcttcccagt gaaagagatt tagctgagca gtttgcggtc agtcgcatga
     2761 ccctccgcca agccgtatct ctattagtcg aagaaggcgt cttagagcgc cgtgtaggaa
     2821 gcggcacctt tgtttccagt actcgagtac aagaaaagat gcgagggaca accagtttta
     2881 ctgaaattgt caaatcccaa ggtaaagttc cctctagcca gctcatttcc tacaaaaaaa
     2941 ccattcccaa tgagcaagaa gttgccaagc taggaatttt tccaacggac aatattatcc
     3001 gaatggagcg ggtccgctat gccgaccaag ttcccctagt ttatgaagtt gcttctattc
     3061 ctgaaaaatt cattaaggac tttaaaaaag aagaaatcac cagtcatttc ttccaaacct
     3121 tgcaaaaaca tggctatcgt atcggcaaat ctcaacagac catctatgct cgccttgcta
     3181 aagaaaagat tgcccactat ttggaagttg aaaaaggaca tgctattctt ggattgactc
     3241 aggtttccta cctagaagat ggtactgctt ttgaatacgt aaaaagtcaa tatgtaggcg
     3301 aacgctttga attttatctt gaaaataatt agattaccac gcaaaaactc tctgtcacgg
     3361 acaaagagtc ttttttattt ttctaagaat aaatctttca aaggaatcaa agttacagca
     3421 aacaatatca ttgccatacc aagaaaatca atgggataaa attgttcatt cattattaag
     3481 aaggcaaaga aaaccgccga tattggctca attgaagcca acaagcttga cttgaccggt
     3541 cctatcagac tggctccttt aaggaaagct gtataggcaa agacagtccc gataaggata
     3601 atgcctgcaa acgcaaggag aaaatcaaga ctagttggga tatcggcctg tagaacccct
     3661 gtaaaaggaa gggcgaccaa acctgctatg accattccca caccaatgac caagctgctc
     3721 ccccactttt taatcaaggc tatgggtaaa atgatataca gagcataagt caaggcagaa
     3781 aagagacccc agaacaggcc agcaggtgtc atggataact ggtccaactg cccatgtgtt
     3841 gcgatcagga aggttcctcc gatggcgaat atgatggaaa ctatctctcc cagtgtcggt
     3901 gccaccctat ccttgataca gctataaatt aaaattccga caggacaaac atactgaagc
     3961 accgtcgctg ttcccgcatt ggtctcctga atagcagaca gataggcgaa ttggttgaga
     4021 aaaagaccaa tcagagcaaa aataagaaga gacagcaaac tctttctatc ctttaaaaag
     4081 accagtattt tatcctttgc agtagcataa gccaagatca tgagaattcc accagcgatt
     4141 aaaagacgca agttggtcaa gaccagagcc gaaattccgt gtgccattag gtattggcca
     4201 ctcgttcctg acaaccccca agcaatacca gccacaactg ttagtaaagt cccttttaaa
     4261 ctatttgaca tgtctctcct tactcttctt tacgaatcaa gttcatcact tgattgcggt
     4321 ctaatatcca ttgagctctc tcgtcctttt cataggtacg attcgatagg aaaatagtgg
     4381 cttcttgctt ctgacgattc cacatgataa aggtacctgt atagcccgta tggtctagcc
     4441 aatctccttc caaattccat gctaaagaac gttccttgtc atccaaagga gaaaaatttt
     4501 gatttaagtc tcttgcaaaa tcatctgcta aatagtgttc taaaaagatt tgtaaatcct
     4561 ttatagtcga aaataaacca gcactcccag catgtctacc caggagacga gccttgggat
     4621 catgcactat gcctgcctct acacctctaa ctgttggaac agcaagctca actggcccaa
     4681 ccttggtttc cgtcattccc caaggtttcc agacttgatc ctttaaaatc acatccaaat
     4741 cttgattaaa aattctttcc aaaataaagc ccaacagcaa aaaatggaca tccgaataaa
     4801 gaaaggctgg ctgacttcgt ctattgagat gaaacatcgc ttcctttaat tcaggggctg
     4861 ttaaaagatc acgattagga ataaaaggat caaggtctgt tgcatgagtc aagagctggc
     4921 gaatagtgat gtctggataa tcactctcag gtaaaaaatc tattaccagt ctatcaatat
     4981 ctaattgacc tatttcccac aagaaggtac aaactgtgcc aaccccaaca accttactga
     5041 cactagctag gtcataaact agtcctgcct cagtctgcaa gccatgctct gggtcactct
     5101 ggcctaaata gaactctgtc cattgattgt ccttaaaata cgcaaaagag gctccgggat
     5161 aaattcctgc ctcgatttgt tcttctattt ttttaataat cttggtccac ttcatacttc
     5221 ttcaaaccac aattcaacat tatttctatc tttacaaagg aagaattttt cagacttagg
     5281 aataaaatat tcagtagact caaacttctg gcgaagactt gctatgtcta attcattgac
     5341 caagaacttg agcatagata agtcccaggt aaccgtattg tccacagtca aatcctgccc
     5401 ctgagctgga ataaaatcaa gggatgcccc aatttcagat gattccaaga aactttcgat
     5461 atcagttggg agatgtaatt ccatagaaat ctcaaattta cttaaagaaa ttgataccaa
     5521 atctgtttga aattcaggct tttctcctac ttctactaga cttgctctgt catcttccgc
     5581 atgaatcaaa atcaaatcat cttctggtga gaaaatttca aaagcgtagc cattttgacc
     5641 tttatataat cgatgaatcg aatctgtttt agataagatt ccttcaattt ctaagggatt
     5701 ttccaccttg acaatcaatc tagctagttt ttttcttccc tctaccttac gagtacgcat
     5761 actgggagct tcttctaaaa ccagcttttc aagacccgtt tggtcaccta gtgacagaaa
     5821 ggccgattct tctagcaagg ccttcattcc aagggtttca atataaaatg tttcatttaa
     5881 ttttctatta ttagctttta aagtaggaat aatccgtaca atctgattta cattcataaa
     5941 ttcctccgtc actttttatt ttaaaggatt ttagaatttt ttacaagata ttatgctcaa
     6001 atttaacatt atttagctat ttagtaaaga tagtctttca aggcttttga caaagcctga
     6061 taaccagcaa tactgaggtg cagtccatca gttgtatatt cttttttgag ttggcctgct
     6121 tggtctgtca aacaatcaaa tactggcaca aattccatct gcatataggc agatgcaagc
     6181 tcttgatagg cttgattcca attctgaatt ttttcattcg agcggatata gactgcctgc
     6241 tggtacttct ctccctcatt gacaggcaaa atggaaagca atttaatctc tgtcaatgga
     6301 taatcgcgag caacggattg aatgatagct tcgagattat tgagagcctc attcacagga
     6361 acatcctttc cgatatcatt tgtcccaatc agaagaaaaa ttttatctac tgctccacca
     6421 tgtagatgag catcaaggtt ctctagtaac agtcctgtct gatagccacg aattcctcga
     6481 ttgacaatcg tctttgaagt cccaaatagc tcctgtagag gataatactc gacaatggaa
     6541 tccccaataa aaagaatgtt tggttctaca acagaaatgt gatttaggtg acgatacttg
     6601 gtttgaattt tttcttgttc ctttaggagc caattttcta ataactgtac tgccacttca
     6661 ttctcctttt tctaaccagt tttccaagac ttgataaact cctccttggc tgttggctgg
     6721 tgctagagca gtcgccacag ctttggcttt ctcatcagca ttttccatcg cataggcaat
     6781 tccagccatt tcaagcattt caacatcatt ttcactatca ccaaaagcca tgatttcttg
     6841 ggatttcaag tcccagcgct tgagtaattc ctccaagccc catgctttat gaatcccagc
     6901 ttggaggata tcaatgcaac catagccact ggatacagct cggacacggc catcaaagag
     6961 agcattgatt tcttccaaaa ccgaactcaa acgttcctca ccaacaacca tgctcatctt
     7021 gagcacacca ccaaagaggt cagatgttaa ttcatccaca aattgcatcc gtttgtagaa
     7081 tttttcaatc atttctggag tcataaaact ttcaagatct gtaaaaatcg taccttcctt
     7141 gacaaaatca cccttcatcc ccgttacaac aaactggtcc tgacacgctc gacccttgaa
     7201 atgagccaaa gccttgttga caatggcgtc atcccatgtc ttagcctgaa tcaattcatt
     7261 gttttcaaaa atacgagcgc cattagcaac aaccagaacc actcgatcca ccaagggact
     7321 cagtagttgt ctcatgcggt gaatttcatt gcccgtcgca atgacaaaac gaatgcccct
     7381 ttgatccaac tgatctaaaa tcttttccaa tcgtgggaga tcaagctgac ctctagcatc
     7441 cagcaaggtc ccatccatat ctgtcgcaat taccttaatc atcatttact ttcctctgga
     7501 ttcaagctaa taccacttca atcccacatg ttcattcatt tctttatagg ctgtatcaat
     7561 tcgttcctta gtcgcttcat ctaactggtc acgatgactg ccaccctcta taaaatcttc
     7621 caaaatataa gtttggtaat gatacaaggg atatccttct ggtgaaaagg ttccctttgg
     7681 tgttcgattg acccaagtag gatgagcttt agctgttccg atagttgttt ttacatcctt
     7741 cttcttgatg gtgacatcca tgagaacacc acgttcagtc cacttagcat tctcttcatc
     7801 tcccatagat tcaattcgtt gattggaaat gaagttcccc attgaataga taatgagctt
     7861 cttatctcca tctttttcaa ccgtttcaga tggttcaaca acgtgaggat gccctccaaa
     7921 gataatatcc gctccccaat cgatcatctt gtgataaaga gctttttgtt cttcagttgg
     7981 ttccaatcga tactcaacac ccatctgagg catgataatg gtgatatctg cttccttctc
     8041 tgcccgttca acttcagcct ttatcttatc ttctcttcgt ttaaatctga aagataacga
     8101 ttatagtctt cctgagaaat atactgctca attccattga aaccatagga ataggctaac
     8161 aatgcaacct tgataccatt cacttcctta atgaccagcg gagcctgatc acgtggttcg
     8221 tgcgtataaa ctccgattgg aatgattcca gctttctcaa taatatcggc cgttgaaata
     8281 actccctcaa tttgcgaatc caaaatatga ttatgagcta aatccagcac atgataacct
     8341 gcctccttaa tagcatccat aacttcagca ggagcattaa agagaggata acccgctaaa
     8401 taatgatcct tattaatggt tccttcaaaa tcaccaatag ctaaatctgc ttgcttgagc
     8461 caaggagtca catactcaaa attttcatga aagtcatagg taccgtcttc ttttttagct
     8521 gaaaagaaaa gtccatcgtg gtagagcaaa tccccgtgtg ccataattct ggcagttttt
     8581 tcctcttcct actcctgaga cttttgctta gtcccctctt gagaaatagt atctcgtttt
     8641 tgactagtca agggattcct tggaagcttt caaacaacaa gaccaagccc attgataaac
     8701 caactgctag caagagtatc gccacaaatc ccttattgct ccaattgcga taactcctaa
     8761 aaagtttacc aagcccttca taaaacgaaa agctaaacca ccctgatttc gattttgtct
     8821 tctttgcatc ttcattctcc ctactttctt atgcaagcct tttcttttta ttatatcaca
     8881 gataagtatt tctttcacaa ttgaattgaa cttcctatct attttctata aatcctaaat
     8941 gccataatgc tttcaatcct tgtcattttg tgatatcatg tagaggtaat gaaaggaaat
     9001 gaactaatcc acagtggctt attccaagta taccacttgg gctttggcag tagctaactg
     9061 cactaaacat aatataagga gagaaaatgt ctgcaataga acgtattaca aaagctgctc
     9121 acttaattga tatgaacgat attatccgtg aagggaatcc tactctacgc gcgattgctg
     9181 aggaagtcac tttcccccta tctgaccagg aaatcatcct aggcgaaaag atgatgcaat
     9241 tccttaaaca ttcccaagat cctgtcatgg ctgaaaaaat gggactccgc ggtggtgttg
     9301 gactggctgc tccccagtta gatatctcaa aacgcattat cgctgttttg gtacctaata
     9361 ttgttgaaga aggcgaaact ccacaggaag cctacgattt ggaagccatt atgtacaatc
     9421 caaaaatcgt ctctcactct gttcaagatg ctgctcttgg cgaaggagaa ggttgcctgt
     9481 ctgttgaccg taacgtgcct ggctatgttg ttcgccatgc ccgcgttact gttgactact
     9541 ttgacaaaga tggagaaaaa caccgtatca aactcaaagg ctacaactcc attgttgttc
     9601 agcatgaaat tgaccacatt aacggtatca tgttttacga tcgcatcaat gaaaaagacc
     9661 catttgcagt taaagatggt ttactgattc ttgaataaag aaaatcccgt tgcaaagacg
     9721 gggttttgtg ttataataga ggcatgaaaa caaatgatat tgtctatggc gtccacgccg
     9781 ttaccgaagc cctccttgca aatacaggaa acaaactcta cctccaagaa gatctccgag
     9841 gtaagaatct tgagaaagtc aaggaactag ctacagaaaa gaaggtgtcc atttcttgga
     9901 tatcaaaaaa atctctctct gagatgactg aaggtcctgt tcatcaaggt tttgttctac
     9961 gagtgtctga atttgcctat agcgagctag attgcatcct tgcaaaaaca cgccaagaag
    10021 aaaatccact tctattgatt ctagatggtc taaccgatcc ccataatctg ggttctatct
    10081 tgcgaacagc cgatgcgacc aatgtttcag gtgtcatcat tcccaagcac cgtgctgtcg
    10141 gagtaactcc tgtcgttgcc aaaacagcca caggtgctat tgaacacgtt ccaattgccc
    10201 gagtgaccaa cctcagtcaa accttagata aactcaagga tgaaggcttc tggacctttg
    10261 gaacggatat gaacggtact ccttgccaca agtggaatac aaaagggaaa atcgccctca
    10321 tcattggaaa tgaaggaaaa ggtatctcta gcaacataaa aaaacaggtc gatgagatga
    10381 ttaccattcc gatgaatgga catgttcaaa gcctcaatgc cagtgttgct gcggcaattc
    10441 tcatgtacga agttttccga aatagactat aaaaaagttt ccagtcatct gattggaaac
    10501 ttttttatga ttaactatgt tctgtaatga atttataggc ttcttgacca gcgatagctc
    10561 catctccaac cgctgttgtt acttggcgaa ggtctttcaa gcgaacatct ccaactgcaa
    10621 agataccgtc gactgcagtt ttcatgtggt tatccgtcac aatccatcct gcctgatctt
    10681 ggatattcaa ttctttaaca aaatcgctaa gagggtccaa tccaacatag ataaagacac
    10741 caccgaaggc ttgttctgtc acttgacctg ttttcacatt ttcaaatacg actgattcta
    10801 ctcggttttc acccttgatt tcccttacta cagaatccca gataaagctg attttttcat
    10861 tcgcaaaggc gcgatcttgt aaaacctttt gggcacgaag ttggtcacga cggtgaacaa
    10921 tggtaacagt cttagcaaaa cgagtcaaga agagtgcttc ttcaacagct gaatctccac
    10981 caccaactac caataaatct tggtcacgga agaaagcacc atcacacaca gcacagtaag
    11041 aaacaccacg actgttcagt tcttcttctc caggcactcc caaaggacgg tgtttagaac
    11101 cagttgctac gataacggta cgtgtttcat atgtttggtc atcagtcatc actttcttaa
    11161 aatcaccatg gtcttcgaca ttttcaacat aaccataaat gtgctcaaca ccaagatttt
    11221 caagtggttc aaacatcttt tcagccaatt caggtccact aatattagcg tatcctgggt
    11281 aattttcgat atcagatgta ttattcatct gaccacctgg cagaccacct tcaatcaaag
    11341 ctacttttag attgcttcga gcagcataca aggccgcagt catccctgca ggtccagcac
    11401 cgataataat agtatcgtac atatagattc cttctttctt gttgtaacta tctttattct
    11461 aactctttct tgtcaatcac gcaaggatta tgccttgaaa acaagaatta aactcataac
    11521 cgcaaaggat aatactggga caaccaagac tccaatcata atcatatcat agagatgggc
    11581 ttggcgagcc ttggctgtct tatcaactcc cgacatggct ctcagaccaa tccaaatccc
    11641 taaaaaaatc agaacaagga tggtggtcaa gatcaaactc tcgaaatata aagaaaatag
    11701 ttgcagtagc atgatttctc tcatttctat cttttttaaa gagtaaactc agctagtcca
    11761 actaactgag ttttccttta tctattatat caaatataag tccgtttgta actagcgaag
    11821 aattcttttg tccgctcttc tttaggggtg tggataatct catccggtgt tccagactcg
    11881 atgattttcc ccttatctaa gaagagaatc ttatcagcca cttgggctac aaaggacatg
    11941 tcatgactaa ccaaaatcat ggtttgacct gacttagcag catctgcaat agacttttct
    12001 acttcaccaa ccaattctgg gtcaagggct gaagttggtt cgtctaagag caaaacatct
    12061 ggtttcatag caagcgcacg cgctagggca acccgttgct tctgtccacc tgataaatgg
    12121 cgaggataat ggttttcacg gtccgaaagc ccaaccttag ccaactcttc cttggcaatc
    12181 ttagtcgctt cttggtcaga taatttcttg acaacaacca agccttcttt cacattatca
    12241 agtgctgttc ggcgttcaaa caaattaaac tgttggaaaa ccatagacaa cttacgacgt
    12301 agggcaagga tttcttcttg agtgatttta gaaaaatcaa ctgaaaaacc atcaatctga
    12361 atagagccac tgtcaggtgt ttcaagataa ttgagactgc gaagaaaggt tgattttcca
    12421 gctcctgaag aaccaatcaa ggctacaact tccccttttt gaatatccaa gtccagatga
    12481 tccaagacag tctgtcctga aaaggatttg cttaaattcg aaatcttaat cattaacgaa
    12541 ggtctccttt cacatctgtt tgcactgtat caggtgcaga aatatccatt tttctctcga
    12601 tgaaacgacc gaggctttca attccgatat tgaccaccca ataaacaagg gcaacggaga
    12661 tgaagcgttc aaaatagcga taatcagctc cacctagaat ctgagcttgg gcaaagactt
    12721 ccacaacacc cgcactaaaa gctagagatg ttcccttggt caaaccgatg agggaattaa
    12781 tcaaggttgg agtagctacc accgctgcat taggaataat cactcgtcga taaacttgcg
    12841 ctcgggtcat acccagactg cgtgccgcct caatctcacc aggattaact gagagaatgg
    12901 ctgcacgaat ggtttcacta gcataagctg cctcattaaa ggcaaaggcg acaatcgcaa
    12961 aagctgcagc tggaatcgca ttgatattga gaccagttcc ccattgctga ttgagggctt
    13021 tcaaagccaa agggattccg tagtaggtca acatgagttg caccaaaatc ggtgtccctt
    13081 ttaagaaact aacaaagaag gcctgcaagg gatataaaat cttgacacga ttgatcttca
    13141 caatggcaaa aagaagcgcc aaaaccaagc caaaaagggc accaccaatt gtcaacataa
    13201 ttgttgttgg aagttgttgg acaattctag ggattccatc aaagaccgaa cgtaggctaa
    13261 acagcttgcc atccggaatc aattgcatca agttttggta ccaatctgat gctaaaatcg
    13321 ttgtaacatt cataaaaaca tcctcttctg ataatgattc attctaccat gcttctgccg
    13381 tcaatgaaat tatttgctac ggtcagatac agactttgtc tacctactag tttatcatac
    13441 tttgaaacag ggaagttata tattttatct atcaaagaga tagataaaaa ctatttcagt
    13501 cccagttctt cataagattt tcgataaccg atttgcttaa cagttccatt ttctactaaa
    13561 atgggccgtt ttaacaacat accgtcactt gctagcaact cagccgcttc tcggtttgac
    13621 aaacttccta ccttatcttt tagccctaat tcacggtatt tgataccact ggtgttgaaa
    13681 aattgcttca attcaaatcc tgaggtttct agccaattca aaatgacttc ttggctaggt
    13741 gtttcttcca cgatatggac ggctttatag tccacaccta attgatttaa ttcttgtttt
    13801 gcttttttac aagttgaaca tttggggtat tcgataaatt ctaacatgtg cttctccttc
    13861 tattgtttct atctttaaaa tctactcctt catgctccaa gagccaagct ttcttttcca
    13921 ctcctgcagc ataacctgtc agacgcttgc ctgctcccaa cacacgatga caaggtacta
    13981 ggatagacca aggattgcgt cccactgctc caccaattgc ttgagcagaa gccacttgca
    14041 ggtcttgagc aatttgtcca taggtcactg tctgaccata aggaatgccc tgtaaatagt
    14101 cccaaactct cttttcaaaa ttcgttccga ttggcgccaa gagcaagttg gataaatcct
    14161 gaggctcgcc tttaaagtaa tcatctaagc aagcaataac tgggtctaaa ataggatgac
    14221 taacaacttc ttctatcgtt tcatctccta gtcccctctc aaaatgcttc tgctcctgaa
    14281 cccaaattcc atacaaataa tggtcatcag ctacaagtga tagaatacca attggtgagg
    14341 agtagagtat ttttacatac ttcttttgca ttatttcttt aatttttgat aggccaagcg
    14401 ttcgccatct actggcacct taccaacttg tttaaaacca agtttttcaa aaatatgttg
    14461 cataaccttg ttttcagcat gcgtatctga gcgaaaatca agataatcaa aaccttcaat
    14521 caagccctct aagaaggttt gagcaactcc ttttccctgc acatctgctg ccacagcaat
    14581 acggtgaaag actagatact ctgactctcc agcttgccag tttccctcat aaatagcttc
    14641 ataggcctcc tctggactct tggtcacagc agcataggct agcagttctc cctcttccaa
    14701 ggctacgtag gcttgccctg agatgatatc atcgataata acatctgcat ttggatagcc
    14761 attttgccac tggtcactac cagcatctgc taaacatttt ttggcatcct ccatcacctg
    14821 catgatggca tctacttcat ttggaaaagc taaacgaatt tccatctttt ttcctcattt
    14881 ctgactagtt tctcccatca tagcataatt taagtatttc tacaagcagt taaaacttga
    14941 ctttgtgttt ttgttatttt ataatctagt tatcaaaact atataaaaag gagttgaaaa
    15001 tgaaaactac cttttcctac ccaaaatggg cagaaattcc aaacattgac ctctatctgg
    15061 accaggtttt gctctatatc aatcaggtct gcacccctat ctctcctaat aaagacaagg
    15121 gcctaacagc atctatggtc aacaattatg tgaaaaatgg ttacctgaca aagcctgaca
    15181 aaaaaaaata ccaacgccaa cagattgccc gtttgattgc tatcacaacc ctcaagtctg
    15241 tattttctat tcaagaaata gcccagacac ttaatactct acaaactcaa gcaagttcag
    15301 accaactcta cgatgctttt gtggactaca tgaaccaagg aattgaccca gctaacccta
    15361 ttatccaaac cagctgccaa accgttaaac tctatcatca aactctagac ttaatcgatc
    15421 atactcaaga ggaggtaatc caatgaacac tagtcttaaa ctcagcaaac aactcagttt
    15481 tggagaggag attgctaata gcgtgaccca tgctgtgggt gcagtcatca tgcttatctt
    15541 gctgcctatt tcatccatct atagttatga agcacacgga tttttatcct ctatcggcgt
    15601 ttccattttc gtcatcagtc tctttctcat gttcctatca tccaccattt atcactctat
    15661 ggcctatggt tcgacccaca aatatgtttt gcgaatcatt gaccattcta tgatttacgt
    15721 tgccattgcc ggctcataca cgcccgttgt cttgaccttg atgaataact ggtttggcta
    15781 tctgattatt gtcatccaat ggggaacgac catctttggt attctctata aaatctttgc
    15841 taaaaaggtc aatgagaaat ttagccttgc tctttacctg attatgggct ggttggttct
    15901 ggctatcatt cctgccatta tcagtcaaac gacacccgtt ttctggagtc tcatgataac
    15961 tggcggactc tgttatacag ttggagctgg attttatgcc aagaaaaaac cttatttcca
    16021 catgatttgg catctcttta tcctagctgc gtccgcactc caatacatcg ctattgttta
    16081 ttacatgtaa aaaagttgag aagttcaatc tcaacttttt tctttacaca tattgataaa
    16141 gtactggtgc aagcgcacat catcagtcaa ttctggatga aaagaactta ccaacatatt
    16201 tttttcttgg gctgcaacaa tttgattgtt cactgttgct aaaatttcta caccctcacc
    16261 aacactactg ataatcggac cacggataaa ggtcattgga atcttgccaa ctcccttaca
    16321 ttctgcttcc gtgtagaaac ttcctaattg gcgcccataa gcattacgct cgaccaccat
    16381 atccatagtt cctagatgac tctctttctg agaagtgatt tccttagcca gcaaaattaa
    16441 gcccgcacag gtcccaaaca ctggtaagcc agatagaatg gcttctcgga tgggaagtag
    16501 catgttctgg tcacgtaaga gcttgcccat ggttgtagac tcaccaccag gcaaaatcaa
    16561 acccgacaag tcactctgat cttgctgaaa atcatctaga tttctgagtt ctacactctc
    16621 gacacctaat tgatctagca cttttgcatg ttctgcaaag gccccttgca aggccaatat
    16681 tccgattttc atctattttc ctcgttcagc catgagaatt tggatttcat tttcattaat
    16741 accaaccatg gcttctccta aatcttcaga gatttgagct aggatttgag gattacggaa
    16801 gttagtcaca gccttaacaa tggcactcgc tcgtttaaca ggatctcctg acttgaaaat
    16861 acctgaaccg acaaagaccc cctctgcccc taattgcatc attaacgcag catctgctgg
    16921 cgttgcaaca cctccagcag cgaaatttac aactggcaat tttccatgtt catgaacata
    16981 ttggaccaat tctacaggga cttgcaaatc cttggcagca acataaagct cgtccttacg
    17041 taagttttga atgcggcgaa tttcctgatt catcatacgc atatgacgaa cagcttggac
    17101 gatatcccct gtccctggtt ctcctttggt acgaatcatg gaagcgcctt cagcgatacg
    17161 acgcaaggct tcacccaaat ccttagcacc acagacaaaa ggaacttgga attctttctt
    17221 gtccacatgg aaacggtcat cagctggaga tagaacttca ctctcgtcga tataatcaat
    17281 ttcaatagcc tctaaaatct gagcttcaac aaaatgcccg attctgacct tagccattac
    17341 tggaatacta accgcttctt ggatttcctt aatcatcttt gggtcgctca tgcgggaaac
    17401 tcctccagct gcacgaatat cagccggaat tcgttccaag gccatcacag ctgccgcacc
    17461 agcagcttct gcgatacgag cctgttcagg attctgcaca tccataataa caccaccctt
    17521 gagcatctgt gccaagtttt tatttagttc ataacgattt tcagtcattt gttttatcct
    17581 catcatttgt attggttgct accttttcat tttaagtcag aacagaatga agcacaagat
    17641 aaaaaaacta taattgtatg gggacagatt ggctaaaaac tatctgacct taacttaagg
    17701 ccagatagct cattcatttt tatttttcag ccgtaagggc agccattgtg atgtagttgt
    17761 atggtttatt gaagtgtggc aagaagaaga ggtctgtcaa tgccaattta tcaattgtca
    17821 catgctcttg gatagcaagt gagaacatgt ggattcccat gctaattgca atatcatgtg
    17881 aaaccatttg ggcaccaaga atttcacggc tatctttgtc aaagacaatc ttaattgcta
    17941 cttcatggtt gtcatgtttc atgaattctg gtttttgaag atcgttaaag cctgtttcag
    18001 ttgcgttgta accagcagct ttcgcttttt caagagtcaa accagttgaa accatgtgaa
    18061 gaccgtagat tgagatacca tttgaacctt gaacaccgat tccttccaat tcatgtccac
    18121 aagcgttgta ggcaccaacg ataccagtgc gaacagcatt tgaagcaaga gcgatatagc
    18181 ttgtatcttt acgagcattg tcataaacag tcgcacagtc accaacagcg taaacgcctg
    18241 ggatagatgt ttcttgtttc ttgtctacaa ggaaggcacc gttgcggaag agttcgatct
    18301 taccatcagc aagggctgtg tttggacgga aaccaactgc aaggataacc atatccacgt
    18361 caaagctttc tttgtcagta atcaagcgtt caactttacc atcaccttcg attgctttaa
    18421 cagtttgacc tagagccaag cggatgttgt gatcttccaa gtttttcgcc atcatttgtg
    18481 tgaagtcttt gtcatagtaa ccgttcaaga cagtatcaac gatatcaaca aggacaactt
    18541 cttttccaag acgttcaaag gcttcagcaa gttcaacacc gatgtaacca ccaccaacaa
    18601 cggcgatacg gtcgagatgt tggctcttgt cagaaagttt attgataact tcttcagcat
    18661 tttggtacaa tttcacgaat tgtacgtttt caagagttgc tttaaattcg cggtttcctt
    18721 taacaatttc aacaccttcg attggtggca agattggtgt agaacctgta gcgaaaatca
    18781 atttctcgta tgattctttg tgttcttttc cttcaacttc tgctgtaacc actttgttat
    18841 catagtcgat tgaaagaact ggtgaattca tataaacctt agcaccttta gcttctaatt
    18901 tgtctttatc agaatagaac aagccttcag caccgtcaat ttgttcacca atccaaagag
    18961 ccattccaca tcctaggaaa gagatgttag agttttggtc aaatacaaca atttcgttct
    19021 catttccaaa attatccaac atggtattga tacatgctgt accagcgtgg ttagcaccga
    19081 ctacaacgat tttactcata aaaaatttcc tgccttcatt ctaaatttac cttactagta
    19141 taccatttac cgttagcgtt tacaaggggg gtactattat ttcagcattt tttatcaaaa
    19201 ataattcccc atttatcctg ttttcaagcc tcatgaattt tatgagcata tttcttgact
    19261 ttttccaaat tttgtttgtg aaaattctaa ctttatggta ttctaataac atgaaaataa
    19321 aatgtaaggg cttcaattta gataattatt ttatattaca acttattttc tataagagaa
    19381 aggagttggt agcttgactc ttcgttcaca ttctgaacgg ctaatgggga ctactatcac
    19441 tatttcatta gtggatgaac aagccgatat ctttctccaa aaatcctttg acttgctcaa
    19501 agagcttgaa taccgcttca acgccaatag tcaagaatct gagttgatgg aaatcaatta
    19561 tcaagcagga atatcccccg tcacggttca tccagacctg tttgagctga tttcacttgg
    19621 gttagagcat agcctagcgc tctctagcca cctcaatatc agcattggtc ccttaattca
    19681 aacctggcgt atcggttttt cagatgccaa ggtcgcccag cctcaagaaa ttgaatcggt
    19741 gctgcctcta atcaatcctc atggtatcga gttagattct tctacttcta ctgtgttttt
    19801 aaaacagaaa ggaatgaaga tcgatctagg ttgtttagcc aaaggataca gtgcggataa
    19861 ggttgcccaa tttcttagga aagagggggt gacttctgcc ttgatcaatc tgggagggaa
    19921 tatcctgacc attggaaaaa atcaggcaag aggggataac ccatggcaaa tcgggattca
    19981 agacccagcc aatcctaggg gaaatcattt aatgaccatc cctgttgtca ataaatctgt
    20041 cgtgacttca ggcatttatg aacgtcacct gaccgtcgat ggacaagatt accatcacat
    20101 ttttgacagt caaacaggat atcctgttga aacggaacta gcgagtctaa caatcatctc
    20161 tgataaatca gtcgatggcg aaatctggac gactcgactc tttggagaaa gacctgcttc
    20221 tatcctctgg caagtcgaaa gtttggaggg catcgaagtc atcctcatcg ataaagaagg
    20281 ccacctaagc tgttcttcag gaattcctac tctatagaga caactgtttc aatggaattt
    20341 taaaattgta ttatgtaaaa agagaaagga aatctcatgt taaaacttat tgctattgtt
    20401 ggaacaaatt caaaacgttc tacaaaccgt caattgcttc aatacatgca aaaacacttt
    20461 gctgacaaag ctgaaattga acttgttgaa atcaaggcca ttcctgtctt caacaaacca
    20521 gctgacaagc aagtacctgc tgaaatattg gaaattgctg ctaaaatcga agaggcagat
    20581 ggcgttatta tcggtactcc tgagtatgat cactctattc cagctgtttt gatgagcgct
    20641 cttgcttggt tgtcttatgg tatttaccca cttttgaaca aaccaatcat gattacaggt
    20701 gcttcttacg gtacgcttgg ttcatctcgt gcccaattgc aacttcgtca aatcttgaat
    20761 gctcctgaaa tcaaggcaaa tgttcttcca gatgaattct tgctctcaca ctctcttcaa
    20821 gcatttaacc caagtggcga cttggttgac cttgatgtta tcaagaaatt ggatgtcatc
    20881 tttgatgact tccgtatctt tgtaaaaatc acagaaaaat tacgtaatgc acaagaattg
    20941 cttcgcaaag atgcagaaga atttaactgg gaaaatttgt aagataggag accgaaagaa
    21001 tgaaatttgt tggacttgtt ggatcaaact acgatcaatc atataaccgc aaactcttgg
    21061 aatttatccg tcgcaatttc aaattcaaat ttgaattaga agtacttgaa atcgacgaag
    21121 ttccaatgtt taaccaagac gaaaaatggg acgaaagctt ccaattgcgt ttcttgtata
    21181 acaagattac acgtgctgat ggtgtcatta tcgccactcc tgagcacaac cacactatct
    21241 cagcttcact caaatctgta cttgaatggc tttcatacga agttcatcca tttgaaaaca
    21301 agcctgttat gattgtgggt gcatcatact atgaccaagg aacatcacgt gctcaagttc
    21361 accttcgtaa aatccttgat gctccaggtg ttaatgccta cacgcttcca ggtaacgaat
    21421 tccttcttgg taaagctaag gaagcttttg ataacaacgg aaatatcacc aacgaaggca
    21481 ctgttaaatt ccttgaaact tgcttagata actttgttaa atacgtagga gtcgtttcga
    21541 aattgaaaaa accaaaacca atcgaatcag aagacttgga ttgtggaaaa ccaatcgcta
    21601 caaccatcac agaagttgac cctgacgatc cagaatgggt agaaaaagtt gcagcaatca
    21661 ctggtgctgt ttctggtgat acctatgtca aattggacca cggtatccta acagttaacc
    21721 aaattgatat gttcttgaaa gctatgccat ttgaattgac atacgctgac gacaacaacc
    21781 aattcctcta ctacaacaac gctcaccaag atccagacac tatgttcgct aaacgtgtac
    21841 cacctcaatc aggtagccgt atgtcgactg ttcatggttc tcttccacca gcacgtatga
    21901 agaacgtaga gtgggttatc ggaacacttc gcaacggaaa ccaagaatac gtccgtacga
    21961 tcgttccagg ttctcctgca ggtgttatca acactcacaa ctaccaagca atgtactatc
    22021 ctgatggatc atacgctggt atcaatgaaa tcgtctttaa cttccaacca tggcttgact
    22081 ggtacctaaa agagactggt caacgtttgg tgggtggtag cggaccattt gctcctgctg
    22141 ccggaggtca tggagacgcc gatgctactt ctggcgcttc tgattcaggg gatgctggag
    22201 gccacggtgg tgacgcagac gctacagccg gtgcaagtta ctaatgatag tttttaggaa
    22261 ccattatggt tcctttttta gcgaaaaaag actagcaaac acaaactgct agtctctttg
    22321 ttatcgtttc atctatcttt gttaaaagaa agaatacata ttatgaattt gggtcatcaa
    22381 gactacggcc atgtaatcct ttttcacgtt gtacttggcg tagtttttct ggtgtaacat
    22441 cgtttccttc ttcgtccaca attttgattc cttcaatgtg gtgacgaaca acgcgacgat
    22501 aaccttcgat gtactcctca cgtagtttgg cttgttccac tttttcttct ggtgttaagc
    22561 cttctgtttt tttcttttta gcaagctcat tgatacgagc aatttttttc ggatccattt
    22621 cttctcctct gtaataagat aaagtccgtt tatcaaattt tatttagtgt taatttggtt
    22681 aaaacaagca aacttagctg ctttcttggt tagttgtgac aagattgcca aacgattttg
    22741 acggacagcc tgatcttcag ccattaccat agtattttca aagaaagcat caatgactgg
    22801 gctaagcgca aagagttgtt tcaattgctg acttgcaggt cctgataaaa cgagtgtttc
    22861 tactgcttct gccaaagctt tttcttggtc attctcaaat agtgctgaat caactgtagc
    22921 aaccccttct gccttctcag ccaggttaaa ggcacgagaa agtgattcaa cagatggttt
    22981 aaaatcttct tccttgctta cttctacgag agcacttgct gcttccaaca tatctgccac
    23041 aacaaagttt gaacctgcaa gaactgcttc cttgatatct tttagagtag agcccatcat
    23101 cttatcaaca cgagccttga taaagtccat aacctctgct ttattttcat aagtcaaact
    23161 gtcaaatttc aatgcataaa ggctatcaat cagctcatcc atagcaatgt gccaaccaaa
    23221 ggcatccaag atacgaacca caccttgggt cgcgcgacga agggcatacg ggtcattaga
    23281 acctgatgga atcaaaccta ctgagaagaa actcaaaatc gtatccaatt tatctgcaat
    23341 agcaagcact gcaccaacct tgctctctgg aagttctcct tcagctgatg taggcatgta
    23401 gtgttcacga atagcagctg ctaccgctgg agtttcacca gcaagaaggg catatttttc
    23461 acccataatt ccttggagtt cgtcaaattc accaaccata cctgtcaaca agtcaaactt
    23521 gtaaatggct gctgcacgag caaggtcaac tgtttcatcc actgacaaac cagctttttc
    23581 tgccaaaagt acagtgattt gacccgtacg aatcatgtgt tcacgaaggg aaccaatctt
    23641 ctcatggaag gtgacattgt ttaatttttc aacaagatct gaaatcacca atttttggtc
    23701 ttcacgccag aagaattctc cgtcttccaa gcgggctacc aagacttttt catttccttt
    23761 gatgacattt ttcaaacgct ctgcgtttcc gttacgaaca gaaatgaagt ttggcaagag
    23821 ttttccatct tgatcacgaa caacaaagta acgctggtgt tccttcatag aagtcaccaa
    23881 gacttcttct ggaacttcaa ggtatttagc atcaaaactt cccatgaagg cagttgggta
    23941 ttcaaccaaa ttcaagactt cattcagcaa atccgcatca atttcgatac gtacaccatg
    24001 ttttgcctca attttcttga tttggtcaac aatcatttgc tcacgttcac atggatctgc
    24061 gattacaaac tgcttacgaa ggtcttcttc atagctcaag gctgactgaa ttttagtttc
    24121 tttccccaag aaacgatggc cacggctcac acgacctccc ttgatatcaa ggaaatccaa
    24181 gtcaaactct tgctcatcca agagaacagt taaagtgtga acagggcgga tgtattcaaa
    24241 gctatttcca gcccagtgca tgctgacagg gaaagtcaat gacttcaaga catctacaac
    24301 accaggaaca atggcctcaa ctgcttgacc aacttcttcc ttagtgacat agacatattc
    24361 ttcacccttg atttcacgga attcgatatc ttcaacagtc aagccttttc cacggacaaa
    24421 tccttgagct gctttggtga agtttccatc actatccaag gcaattttct ttgctggtcc
    24481 cttgaaatct tctgtcaaat cagactgttt gtctgataaa ccagtcacac gaacagccaa
    24541 acgacgtggt gttgagaagg tttgaatggc ttcaaaagac aggcggtttt ccttgaggaa
    24601 ggctgccatt ttttcgccta gttgtttttc acttggtgtg acaacatagg ctggtaattc
    24661 ttcaagaccg agttctacta ataagttttt tgtcatgttt tctcctttac attttcttca
    24721 atcttttttg atatgcacct taggtactgg ggacgtcgct aactaactct gtgagtctgt
    24781 atggaaatcc aatgctaaat ttatcaagat ttccaacaat ctcatcaaag tggatttagc
    24841 tgactgattt ttgaacctaa ggtttcaaaa atccccacat aacagtacgg cggtgcatat
    24901 ccctctagca agtcttcgac ttgcctctaa cgattttaga gtacagtatt actcactatt
    24961 ctgcgtcttc tgctaaaagt ttaactcgtg tttcttcatc caaaagtggg taacctaggc
    25021 gtttgcgttc tgcaacaaag gttttggcta caacacgggc caagttacgg atacgagcga
    25081 tatagcctgc acgctctgtt acagatacgg caccacgcgc gtcaagcaga ttaaaggtat
    25141 gtgaacattt gagaacatag tcataggcag ggtgtaccaa gccttcttct aatgcacgac
    25201 cagcttcttt ttcaaactta tcaaagtttt caagcaacat ttcttggtcc gaaatttcaa
    25261 atgaatattt tgagtgctca tactcaggct ggataaagat ttctccgtat tttacaccat
    25321 cagcccactc gatatcatag acagagtcta cttcttgaat gtaagaagcc aagcgctcca
    25381 aaccataggt aacttccgca gtcacagggc cagttgccaa tccaccgact tgttggaaat
    25441 aagtgaactg agtgatttcc attccgtcaa gccaaacttc ccaaccaaga ccagctgaac
    25501 cagttgatgg gttttcccag ttgtcctcaa caaaacgaat atcgtgctcc aaaggattga
    25561 ttcccaattt ttccaaagac tcaaggtaaa gttcttggat atttgatgga gaaggcttca
    25621 tgaccacctg gaattggtgg tgttggtaga gacggttagg gttttcccca taacgaccgt
    25681 cagcaggacg acgtgatggc tctacataag ctgcattcca tggctcaggt ccgatagcac
    25741 gaaggaaagt gtaaggactc attgttcccg cacctttttc attatcataa gcctgcataa
    25801 gcatacaaca ttggtcattc caaaattgtt gcaaagtaag gatgatttcc tggaaagtca
    25861 atttcttaga cattgtttac tcctttttat ataaattact tgcgacatga gtctgcctcg
    25921 tcgattatac gaggcaagac gagttagtac tgcgtctagc tcaggcactc tagtgctgag
    25981 cgtggggcag tccgactgta aggaggtttc tgccactgat gcagtgaaat gttaatttct
    26041 tagacattgc ttactccttt agtttttata tttctttttc aattaatttc tgctgaacaa
    26101 accaatttaa caagggaaga gttttatctg tatttgccca tagatgataa gaatctaaga
    26161 ccgcttgtac actacctaaa ttttctacca acttatctac tgtcaaaaag ctgcgccagt
    26221 attcataaaa aatacttgca tagttcttat cataagtcga agaaccaaaa tcattcaatg
    26281 aatgccaacc atacttcttc tgaaaaagtt ctactagaga ttgattacaa attttttccg
    26341 cttgaaattc ctcttctgtc aagaaatact tgcgactaat atattcaacc atcccctctt
    26401 cgaaccagat ataagagtca gaaccatcaa aatcatctaa aaaatgctcc gaccaatgag
    26461 ctaactcatg tcctacaata tgtaagagga aattttcaga taaagaatga tagtgacttt
    26521 ctattgcttg agtttgctca gaacactcgt agtccatcaa ctgatacaaa tacaactctt
    26581 tccaaacagc taaatcaggc gtcataacca ctcgtctatt atttgtatag gttggaacag
    26641 cactttccct aataatctgt gtagcagcat taaaatctga ccaaataacg ggttgcggta
    26701 aatcacagac tgcaagttca ttctttaaaa aagattgata atctttcaac ttttcagtat
    26761 ttcttactac aaaatcacga aatgtagcta gttgactatc gtcttttaca agataaaggt
    26821 tgtccacggc tctcctttct ttcacacaat agactgaata agcaaaagaa ccacatctga
    26881 caatcctagg ttgatttaac ctaggggcgt cagaaacgcg gttccaccct aatttattac
    26941 ttcattgttt ttgaaaatac tacagaaagc gccagctcat tattttgccc tacttggctc
    27001 ccactgtccc aagctcgctt gaagaacgcc ataagacttt ctttcctgct gactattata
    27061 tcaaaaatta gaataatgta caattctttt tatgattttc tagaaatcca catcatcaac
    27121 tcgtggagcc cccgactgaa cagcagtcgt ttttaaggtt tctctctcct catgactcag
    27181 ttcaattcca aaacaatcaa gattagcttg aatacgagag gttgtgacag attttggaag
    27241 tggtaaaaat ccttctgcca agctccaggc caaggctatc tgagcaaccg attttccgtg
    27301 atttgctgct atttcttgga cttgcttgct atcaaacagt tctccttgtc caaaaggccc
    27361 ccaagcttcc aataaaattc ccttttcacg acagtaagct acgacttcct cttgatacac
    27421 acctggcgcc aagcgaactt gattgaccgc aggaacgata gttgcagttt caagcaaggc
    27481 atccaaatga tggggaagaa aattgctaac gccgatagca cggattttcc cttcttgata
    27541 gaggtcttcc atcgctctcc aaacttccgc attgcgagtt ttccatgcgt cattttctct
    27601 gagcggtttt gggttcggcc aatgaatcaa atacaaatcc aaataatcca agcccagttt
    27661 ttctatagat ttttccaaag cttgacgagt ttgctcatag gtttgctgac tattccaaag
    27721 cttggtagtt acgaacattt cttcacgtgg aactccgcta tctttgattg cttgaccaac
    27781 actttcttca ttctgataaa tcgccgccgt atcaatatga cgataaccag ccttcaaggc
    27841 ttctaacact gcacgatagg cttcttctcc atccttagcc ttaaaagttc caaatcccaa
    27901 tactggaatt tctactccat tatttaattg ataggtattc atatgcttct ccttctattt
    27961 tctttatcat ttatagcata atttttttgc taattagtac tatcttctgt aaaaaataag
    28021 tctatcacat atctgacaga cctatttttt cttgcttatt catcacgact atagtacagc
    28081 tcatgagctt ttaggcgagt attgagcatc tctggtatgg tcacaaaggt ataaccttga
    28141 tttttcaaat actcaatgac ccttggcaag gcattgactg tcggactgtg aatatcatgc
    28201 atcaaaacga tagatccgtt agctacttga tgctgaattt ctgtcaaaat agatgcttca
    28261 tttttactct tccagtccag actatccaca tcccacatga taaagctcaa atccaagcta
    28321 ttgcgaatat catctgtaat agcaccataa ggtggacgca tgagtttaga actagaaccc
    28381 agcactttag ttagcacatc ctcagtatca gtaatctgct ttttagcttc atcaagagag
    28441 agttgcgaga gaatcggatg gctccaacta tggtttccaa caacatgacc ttcagatttt
    28501 atcctcttca ccaagtcctc attcccagaa acatttttcc caagcacaaa gaaagtcgct
    28561 ttaataccat atttagctag ggtctctaat acctgcgggg tcgttgctgg atttggacca
    28621 tcatcaaagg ttagagcgac aactttttga tatttcttat caaagtaaga ttggtacaag
    28681 gccgcatctt tttcgagtaa gtacgaagat tggataacat caaagaaagc agatactggc
    28741 aaggctatct cttctaaatt ttcaaccaca ggacttggat aaaggataat ctgactatcc
    28801 ttgtaatcaa aattccatgc agacaagtct tggtcagaga agttttttac aatctgctca
    28861 ctctggtctt gctctatttt tttatcctct atgaaggagg tcaactcttt tatcagctgt
    28921 tccttagcct tactagcatc tgaaaacagt tggtcaagtg taaaaggttg cccatcttct
    28981 gttaagtgaa cctttcctag actggtcttt tcagtctctt caaccttaaa agcaactaaa
    29041 tcatagattt gtttggtcac attacgatta acgactccct ttaaacctga ctcttcttgc
    29101 tctgtatagt agaaaacaag attgtcctta ctttctagct tgtccttaac atcttgattt
    29161 atcaactccc taacagagga aatcactttc tccccttgga gaggataata ggcaatcact
    29221 tcggcttgcc cctgacgaaa atgctccttc tgatttccct cactcaattg atcatctttc
    29281 tcttttttga gcgattcaat cttttgctca aaactttttt gctggtagat cttaaaagca
    29341 ataaatccgc ctaataggca aatagaaatt gctaaaatac caatcaaagc caataataca
    29401 tgtctcgttt tcccgtgtct gccacgtcct agtctacttt tattcatatt tatatcataa
    29461 caaatttgag caataatttc aatgatacaa tttggcaatc cacaattcct ctagcaagcc
    29521 aaagcatctt acatcaactc caacatccgt atctgtagag attatgtagt tttctatcta
    29581 tggaatttct agtagattga agctagaata gtagacctat acttctaaaa cattgttaga
    29641 aatcgatttg attgtcctga tcgatttgtc ctgttctcat ttcattttac tataaaacta
    29701 tgaaaagcta ggtcatctga gaagtaatta ctatcgacaa ttgcgatagc gtatactggt
    29761 gcgatttgtt tgtagctctg gtgggtatca ccttcacgtt ggcgaatttt ttctaggttt
    29821 tgattaacct gactgcgcag ataagcccat aggcgattga tgaaaaaatt ctgatgatga
    29881 acctgaattt cgatgataac ttagattcca ttatctaact ccgccaagac gtctatacta
    29941 gtatagaaat cctgcgctga gtagggcatg gaaggcaaga cgtgaatgtt acttccctcc
    30001 aaaatcgtca cattttttgc tggcaagtcc agcatatcgc ggataaattg acaaatcatt
    30061 agttgggctg atacccggat gtctgagaat cctccttttc ctccttctat tatagcacat
    30121 ttttaaatct aggtttgcta gattctatgc ttctatctat ttattcggaa aagaaagctt
    30181 ttcttcagaa gaaagctagt agcttatttg aaattttctc ctctctgttg gatagtcccg
    30241 tctatctatg ttataatgaa agaaggattt aaaatcggaa aaggagtatt tatgctaaaa
    30301 ttaggtgtca tcggaactgg cgctatcagc catcatttca tagaagcagc ccataccagt
    30361 ggagaatacc aactggtcgc aatctattct agaaaactag aaactgcagc aacctttgct
    30421 tctcgctatc agaatatcca actattcgat caattagaag tcttcttcaa atcttctttt
    30481 gatttagtct atatcgccag tccaaactcc ttacattttg ctcaggcaaa agctgccttg
    30541 tctgctggta aacatgttat tctcgaaaag ccagctgtca ctcaaccaca agaatggttt
    30601 gatttgattc aaacagctga aaaaaataac tgttttatct ttgaagcagc tcgtaattac
    30661 cacgagaaag cctttactac tatcaaaaac tttttagcag ataagcaagt gttaggagca
    30721 gatttcaact atgccaaata ctcttccaag atgcctgact tgttggctgg acaaacacca
    30781 aatgtctttt cagaccgttt tgctggtgga gccctcatgg atttggggat ttatcccctc
    30841 tatgctgctg ttcgtctttt tgaaaaagct aatgacgcga cttaccatgc tcaacagctt
    30901 gacaatagca ttgacctaaa tggagatggt atcctcttct acccagacta tcaagttcac
    30961 atcaaggctg gaaaaaacat cacttccaat cttccttgcg agatttatac aacagatgga
    31021 accttgactc tcaacacgat tgagcatatt cgctcagcta tttttaccga ccaccaaggg
    31081 aagcaagtcc agcttcctat ccaacaggct cctcatacga tgactgagga agtcgctgca
    31141 ttttcacaca tgatccagca accagatctg aatctctacc aaacttggct ggatgatgca
    31201 ggctctgttc atgagctact atataccatg cgccagactg ctggcattag atttgaggca
    31261 gaaaaatgaa aaccaaacta ccgactgaat ggcaagaact gagtgaccaa ctcggtttcc
    31321 aagaattcac ccccattcaa actcaactat ttgagcccct tcttgctgga gaaaacctcc
    31381 taggagtgag ccaaaccgga actggtaaga ccctagcgta tctcctacca agtcttctca
    31441 gactacaaaa gaaaaaagcc caacaactct tgattctagc accaaatacg gaacttgctg
    31501 gacagatttt tgatgtatgt aaaacgtggg cagaagctat cggattgact gctcaactct
    31561 tcctttcagg ttcaagtcag aaacgacaaa ttgaacgcct taaaaagggg tcagaaattc
    31621 tgattggaac tcctggccgt atctttgaac tcattaaact taaaaaaatc aagatgatga
    31681 atgtggaaac catcatcctg gatgaatttg accaattact agatgattct cagattcact
    31741 ttgtagagaa aatcactcac tacgcacctc gtgaccacca actggtctac atgagtgcca
    31801 cgaccaagtt tgaccaagaa aagattgtgc cgaatacacg cactattgat ctctctgatc
    31861 aaaaattgaa caatatccag catttctaca tgcaggtaga ccaacgtcat cgagtggata
    31921 tgctacgaaa actggctcat gtagaggatt tccgtggtct agtcttcttc aatagcctgt
    31981 cagaccttgg gaatgccgag gaaaaactac agtatcgtga tatattggct gtttccctcg
    32041 ctagtgatgt taatgttaaa tttagaaaaa tcatcttaga aaagtttaaa gataaccaac
    32101 taaccctgct tcttgcaact gaccttttgg ctcgtggaat tgatatcgat agcctagaat
    32161 gtgtcgtaaa ctttgatatt cctagagata gcgaaaccta cacacaccgt gctggccgta
    32221 ctggtcgtat gggcaaggaa ggttatgtta tcactctcgt cactcatcca gaagaaatca
    32281 aaaaacttaa aaagtttgca agtatccgag aaattgtcct aaaaaaccaa gaactctata
    32341 tcaaataaag ccccattgct ctatacacat ccagtgtatg gagctttttt ataagcaatt
    32401 ca
//
LOCUS       DAVKDZ010000018        31945 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000018 DAVKDZ010000000
VERSION     DAVKDZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31945
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.018"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(<1..2669)
                     /locus_tag="VWZ30_000817"
     CDS             complement(<1..2669)
                     /locus_tag="VWZ30_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU9334593.1"
                     /translation="MDQEKGRPKTIGHRLDRKLEKVRKNYRTVQQDSAKQLTKLQSQT
                     HSKRQLVKKSRSQFRASKNKVKKIQKEYKRIQKEYKNIQKQGRNLGKEGKSLRPEEIK
                     KQVFLKQELDRNNKKRVFLKQELDKAKLEKKTSKRTFKVAQEANGGSRKKKFLRAAKQ
                     SVERVANQQASSVAHQDDTLSDIARTKYRYQDIQIQGKRIKTIGKYSGKLGQGMVKSS
                     YSLGNRVYNKAKGRGFTQTPREFSWEGKLSRRIQDYKKRLATSKTGKVAKKARKFYRV
                     GSKPLKVIIKNPFSLKAYLIAFGLLLFLAILGIGSSGITRQDEFDMNDTWLYLSKLDR
                     EKSNDKVDYWTKIDDPLLYLNYKYDDISDKLRIDGNNYFSQQNRGKLYLDTLWKNLNG
                     DKDNLKTMEELYTKNNLYKLDKDELEEYKELLEIARDSGKYMLLQELDNPFYTEEQPE
                     SQAPLQIIERFGYKSKTEVFNGSVLQVSGGQSLLAVLDGKVEVKGSDIEISDQDSKFL
                     YKNVDTIRYKTGDHVKAGDIIGKVAPEGNQTVYYQKLEPDRLNKKDKDGNVEKSWTYV
                     NVGFYFQRVEYTQATSVVSNIETSGEKGRRARAFADAIKKNIPEATDEGIAAVLGGFD
                     IESSITFKRYETDYLTNNQFDKVAKEPTAENLVGNWGAFQAMYPTLPLNERGYLVNGL
                     HYIGIGIGQFTGPRALALWNFAKSVNGDIWSAEVQTKFLLEEDDPTRRAAFRRIVTST
                     GSVEALAEDFLNAWLGVPGNKLLERQNAARQWLNFLKNKGGGSTGVSSKQVPAEYKDK
                     LPYGLPTDQALLEGQGYSGNAYELGNCTWYVYNRFAQIGVGIHPYLGNANQWVDSGQA
                     QGYDISTTPKPGSAAVFMNGVAGASPIYGHVAF"
     gene            complement(2680..5043)
                     /locus_tag="VWZ30_000818"
     CDS             complement(2680..5043)
                     /locus_tag="VWZ30_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEU9334594.1"
                     /translation="MSLFKSKREKLEKAEKKKIDRLRRQMKPTTQNTINYTLLHEDGL
                     MHIAREKYSRSFMLGDASYSTAQQDEKESIIDTTMDALNSLDEGSNYQLLVINRRVSD
                     NSLQNILYEETNDGFDDYREELNEMIKERFTTHANNFEVHKYVTISTNADDRKQAQLI
                     LQDIGVSLGTQFQESDITFRDMNGLDRLKVFSELLRDNPYVNFDYKEIALTGLFTKSF
                     IAPNRIEFQEKRMIIDDRWCKVLYARRYPNWMSDRLIRDLTTLGLELAITIHAEPYEN
                     VEILEKIDDAETDAEIGKLRSKRKAAQSGIFDDAVVAGRDQEISEATKKWRDEITEYD
                     QKIFSGLIAVYFKADNQEELQRNEQKIKRAGRKLGVEFEDLYYYQEEALNTVLPIGEC
                     FLNVKKNFMRKMTTANIATQVPFTNVDLKSESSLARYYGQNQLSNNIITLDRKRDLNT
                     GSGVVLGSSGSGKSTTIKGGEVIPTLLKYPEDRVIIVDPEDEYSDIGRAFGAQMVDIS
                     IGSKTHINLLDLPDLDRLDDEDDDPIGDKANLLMGLFESILSEVTDAQIGIIDRVTGV
                     TYERYLTENFTPTLKEWHQVLKEQPEPEAQDLALAVETYTTGSQDIFAHNTNVDLNHR
                     FVIFNLKKLSHKLKPFALMVIQDYIWNQIVENQGKCTTWVYFDEMQILFKNKAQADFF
                     TTLYSRIRKYGAIPTGITQNVETLAAVEEGRKLLSNSEFMILLKQKPQDLAILKEIVN
                     LTDKQIRYLARPKAKGTGLIVAGPVVVPFENPIPKNTKLYNLVATDA"
     gene            complement(4985..5359)
                     /locus_tag="VWZ30_000819"
     CDS             complement(4985..5359)
                     /locus_tag="VWZ30_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334595.1"
                     /translation="MKKIGSEFLREFETEDKPILFGLTSRMLVLYLCLIIAVSGTTAS
                     YFFGLPDWYIYLWDALFVVPGGMYGTGMTKRMRIKEKINFFFRIQQRTYEIEFGKVGM
                     YTKDEFIQKQKRKTRKSRKEKN"
     gene            complement(5458..5961)
                     /locus_tag="VWZ30_000820"
     CDS             complement(5458..5961)
                     /locus_tag="VWZ30_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334596.1"
                     /translation="METLKEIGNKQFNDLQKKHGTRELKDKITSLEQEITRLSWFAYE
                     HELLSEPLLEWILDGKVKISEIPRAVRMSSYGDELYIYAWRYAEAKQDAFYGMRILTL
                     LQEDITYCAIADSISQTEYVYRLEQWIKYMDRGKMVFKGDENFERYFQEQKTANRSLF
                     DTEGVGI"
     gene            complement(6049..6798)
                     /locus_tag="VWZ30_000821"
     CDS             complement(6049..6798)
                     /locus_tag="VWZ30_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL"
                     /protein_id="HEU9334597.1"
                     /translation="MNLDIRELTSSLSSYGSATNQAVNMMADAVRPIGYILLGLFFWF
                     EMASWSQMVKSRGGSLTQKIWLEALMKYLFAFIMISASSQICDAILELLNIIVKVIAH
                     VTPTVKATIDSNISGGKGIVEKTIITFVGKAVSGVANVILGIIVFLRYLDLYMLKALA
                     PLLVAFFMMDSLRSVTINFLKYFAASALIGVILIIVSVVYDSIVAADILKVTTGSSDS
                     WGTAFASIAKGVIYIFVMVGSSRKAKQLLGV"
     gene            complement(6808..7035)
                     /locus_tag="VWZ30_000822"
     CDS             complement(6808..7035)
                     /locus_tag="VWZ30_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334598.1"
                     /translation="MEIITKIITGLGVVGTITGLIWIWNGAVDYIQGRKNKDKQRQDD
                     GSDSMINGAFLAVASAGIAAAVVAALSQLKF"
     gene            complement(7118..7537)
                     /locus_tag="VWZ30_000823"
     CDS             complement(7118..7537)
                     /locus_tag="VWZ30_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334599.1"
                     /translation="MKFGSDFSWLWVAIIRIFTAPFYIVLWCINVVKSTIGMFILWVI
                     AKICITIVLIGGMAIIHHLFNFPSENIIDNIFGWYTPHILGMSHDSLITAGQVVDAPK
                     GGGLFFPYPNFEVPIIIGLSILVATVRTIYREEFEEL"
     gene            complement(7609..12054)
                     /locus_tag="VWZ30_000824"
     CDS             complement(7609..12054)
                     /locus_tag="VWZ30_000824"
                     /inference="COORDINATES: protein motif:HMM:TIGR04228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9334600.1"
                     /translation="MNRINQKNASIRAENEKNQRENQAEIDRINQENAEIRKRNEAKR
                     GAYESSLTDYTKKLATIKAERDAIQTSKPLFGSETGFKVYGGYNSAGRGSLDYYNDFT
                     VVPDDNLPVESMRGFLGYHADTYVTGGAGTRVSKDSTETYDIIKSPTFGDTFYIHNIG
                     TLTDGRKIMAKVMVSDLGDYQGEVRNGVPVTDSDIYLKGGDGGSFYFVYNNHTRLEMV
                     FDFYIEGTTTPVSLLIGTVITDVDWGQGSNLSYGSSGRGMVLNPSGSGLDFDGRVMKG
                     VENGVNDTSDIPKASFASVGYGSSLTYLHTSSPGSTEGRTPAEWDAENLSGNVQNVVF
                     TILGEGAELKSIPPVNPPRKPTYEVETTPPNSPTGKPEEVLPPKPEEIKEKEIPSLVS
                     PPTVRVRYARLQAMPDLEKFVKNSSGESIDKSYVPKLSTVQWELTTKPLPANREAITD
                     FEIVDALPSGFVLDVEASKKISSDFELTYDESSHVVRMKGLESLKSKLNQDLSKEVQV
                     PAPILVGKVTNDGATYKNNFQLKINNKYASYSNIVQISTPGKPNDPDNPNNNFIQPLK
                     HNYNKDKVIIDGKPVLVGSTNYYHITLDYDQYKGMKADSSTILKGFGAIDDYPEEAVT
                     INQSDIRYIDSEGKEVAGISVYQYDSIDAVDNDKVKAFLASSEIKPKGAFQVFLVDDP
                     EAYFNQYIKSGKSVTIIDPMVTKEELRNTGKSFENTAYQVDFGNGYQTDTVVNNVPTV
                     KPTKKNLNKAGVNIDGKQVLAGSVNYYKVTADYSQYKGIEADKDRIGKGFYIVDDYPE
                     EAVTINQDGVQVTDSKGQVVKGLKMALYDSLDKAPSGVQESLESSHFTPKGAIQVFEA
                     ENPEEFYKTYVQAGEVLTITNPMTVKKELGQTGGKYENTAYQLDFGSGYQTDKVENNV
                     PTAKPTKKNLNKAGVNIDGKQVLAGSVNYYKVTADYSQYKGIEADKDRIGKGFYIVDD
                     YPEEAVTINQDGVQVTDSKGQVVKGLKMALYDSLDKAPSGVQEALKSSHFTPKGAIQV
                     FEAENPEEFYKTYVQAGEVLTITNPMTVKKELGQTGGKYENTAYQLDFGSAYITDTVV
                     NNVPTVKPTKKNLNKAGVNIDGKQVLADSVNYYKVTADYSQYKGIEADKDRIGKGFYI
                     VDDYPEEAVTINQDGVQVTDSKGQVVKDLKMSLYESLAKAPSGVQEALKSSHFTPKGA
                     IQVFEAENPEEFYKTYVQAGEVLTITNPMTVKKELGQTGGKYENTAYQIDFGMDYVTE
                     TVVNNVPKIEPKKDVVIDHLSKESLDGKEVKLNQTFNYKLVGSLVPKDRSEQLFEYKF
                     SDDYDETHDDYQSVYKVFAAVDFETSDGQKFKAGDELTKFTSQVVDKAKGKVDISFDD
                     ALLKSILETSEFQAEVYLQMTRIQSGAVENTYYHTVNGVEVASNTVVTQTPEEPKTPE
                     EHPQQPERSLPSTGEQASAELLLAGLAMGGAASGLLYSKRKKKEA"
     gene            complement(12072..12578)
                     /locus_tag="VWZ30_000825"
     CDS             complement(12072..12578)
                     /locus_tag="VWZ30_000825"
                     /inference="COORDINATES: protein motif:HMM:NF037874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334601.1"
                     /translation="MHKARETKTYGSIRKSKIYGTCGVILGLAALSMISPVIADERTE
                     NKATNAPYAQTSPSSISTENQGKSEEKTGTLEVSISHSSLDETIRKAQEAGLKVEFDS
                     VVDKGTASTASELEKSKRKSRVIIAHKQIVLRKLLKNIVKIKDKIKRTERKSKMKILR
                     RRNNIKRI"
     gene            complement(12724..14658)
                     /locus_tag="VWZ30_000826"
     CDS             complement(12724..14658)
                     /locus_tag="VWZ30_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC10/PgrA surface exclusion domain-containing
                     protein"
                     /protein_id="HEU9334602.1"
                     /translation="MKLRTTILATTASVTLLGLGNSQPVYANSTTSSQVESLKSEFIK
                     AKREYEQAKSIYDNALSSSPSNTIILSDKYIKALKTAFSDFNISQTERDSAKSILQSE
                     SLRLKNQNSFHKDVADEGERLDVNNLPLAVRQELSFFAQDLINQVRSQVGTPRVSVSI
                     SALDFADKVAKAYVQDNWGWHKMSVSGTLGHDATGINHVAREYGLPTTNSEEEKKGEQ
                     NYENLASRLPGFKTANKAQLKEAIYIGMIEFMFNDTEWMHAQSIAGLNWGNVNSKDYF
                     GLSFSSRSSVSSAHFITVSQEDIKRASKSSFSTAAVSDPTSVNRRQAIKKLEEDYKAK
                     EKIYQDFQKQADSKGSQGQSNQGSATVTEPSKPSAGSAEPTKSIENTSDLRDQWKQEG
                     SYWYYFDRAGKALVNSWKGNYYLKSNGVMARNEWVYDTNYKAWYYFKSDGSYAQNSWQ
                     GSYYLKSDGKMAQSEWLYDSSYKAWYYLKSDGSYAQNSWQGSYYLKSDGKMAQSEWLY
                     DSSYKAWYYLKSDGSYAQNSWQGSYYLKSDGKMAQSEWLYDSSYKAWYYLKSDGSYAQ
                     NSWQGSYYLKSDGKMAQSEWLYDSSYKAWYYLKSDGSYLRDQWFKDGSAWYYLKADGK
                     MAQNETIGAYYLDYSGKWIS"
     gene            complement(14850..15164)
                     /locus_tag="VWZ30_000827"
     CDS             complement(14850..15164)
                     /locus_tag="VWZ30_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334603.1"
                     /translation="MNQSKLVTEVTLADLRRLGNQGNATARLDNGDEIKLTSRYGLVP
                     KKGYLAGKLETVWMIVEYSKIYKEIRTIKRGDVLVVRRIKQGNTNRLLLTGKGYHRPT
                     KH"
     gene            complement(15476..16570)
                     /locus_tag="VWZ30_000828"
     CDS             complement(15476..16570)
                     /locus_tag="VWZ30_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEU9334604.1"
                     /translation="MADITVNQVATQTFYQIPQIFMVNVKKKFSAEGKPTGKIKMTSL
                     YAKKLSNDGKIAYGVLYDRCMLSIYSYRNGELTYVDENGSVFLVYTVEDLMEILDRSK
                     PTVLKIKKELKELGLLREVKQGKNRPNRLYLQNVNAELQEYEHYDAEVVEKGRDKGTV
                     VYHHVKTLDYKGCVLFEIERPGSEEESSILKDENSVLEKPIKSINSGSQKSLPPEVKN
                     FNPIKTEKSHTENNSTRHSSRKPASNSKELDQTAGTCDTPSQKTSGKKFIKPQVYSLL
                     QEIADSYRDHLPWYQHYNMTHRQKMIVGQYIQTGWITSSEVLHCIERIPECDSPFAYL
                     IQSIENLIGERKSEAKMQAHREAEYRYLSN"
     gene            complement(16575..16739)
                     /locus_tag="VWZ30_000829"
     CDS             complement(16575..16739)
                     /locus_tag="VWZ30_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334605.1"
                     /translation="MTKEMFVQFSKKAAVNEVMRKTGMSKKRAVKAVEELEREGVLFS
                     MGNGTVMMGV"
     gene            complement(16736..17014)
                     /locus_tag="VWZ30_000830"
     CDS             complement(16736..17014)
                     /locus_tag="VWZ30_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334606.1"
                     /translation="MWLETIVCFDCDYEEKWEEDNIFKPIHCPECRDVLDVVERDWLG
                     ETILRSTVQCERCNFLFDHLDFQLGLSECPSCFSKELKLVRQKKEIVE"
     gene            complement(17281..17610)
                     /locus_tag="VWZ30_000831"
                     /pseudo
     CDS             complement(17281..17610)
                     /locus_tag="VWZ30_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282476.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17829..19013)
                     /locus_tag="VWZ30_000832"
     CDS             complement(17829..19013)
                     /locus_tag="VWZ30_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334607.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIVLLLFASVLGVTFYLNSQTANSVS
                     LESRLESRIVDNGRAINENEAKLSQMSDTSSEEYQFAKNNLDLQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIESWGSSRKKEVFN
                     RS"
     gene            complement(19010..19603)
                     /locus_tag="VWZ30_000833"
     CDS             complement(19010..19603)
                     /locus_tag="VWZ30_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9334608.1"
                     /translation="MLQLTHVTLKTRQVILQDVDFTFEKGRIYGILAINGSGKTTLFR
                     TISNLIPISSGNIAAPPSLFYYESVEWLDGSLSGIDYLRLIKNIWKSDLNLRDEIAYW
                     EMSDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDVCDRVVTIHQGQIEEVPL"
     gene            complement(20003..22243)
                     /locus_tag="VWZ30_000834"
     CDS             complement(20003..22243)
                     /locus_tag="VWZ30_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU9334609.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSVSYFALLGFVFLLVCL
                     FIQFPWKSAGKVLVICGVFGFWFLFQTWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSDGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     VFLPLLSILFALSFLYPVIQLNFIFEWLEGMIRLVSQVASRPLVFGQPNAWLLILLLI
                     SLALVYDLRKNIKRLAVLSLLITGLFFLTKYPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESEKKIEKWQEKATTSNAQRTLIPYLKSRGVSKIDQLILTNTDKEHIGDL
                     LEVTKAFHVGEILVSKGSLKQKEFVVELQATQNKVRSVSVGENLLIFGSQLEVLSPRK
                     IGDGGHDDTLVLYGKLLDKQFLFTGNLEEKGERDLLRQYPDLEVDVLKASQHGSKTSS
                     SSAFLEKLKPEITLISVGKSNRTKLPHQETLTRLETINSKVYRTDQQGAIRFKGWKSW
                     KIESVR"
     gene            complement(22227..22877)
                     /locus_tag="VWZ30_000835"
     CDS             complement(22227..22877)
                     /locus_tag="VWZ30_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU9334610.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            complement(22944..23513)
                     /locus_tag="VWZ30_000836"
     CDS             complement(22944..23513)
                     /locus_tag="VWZ30_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9334611.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYTSDKGNTRY
                     TFPTNQILEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTPETNRAVIELAFEKIGMNKLVALYDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            23690..24801
                     /gene="ald"
                     /locus_tag="VWZ30_000837"
                     /pseudo
     CDS             23690..24801
                     /gene="ald"
                     /locus_tag="VWZ30_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253293.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            complement(25168..25428)
                     /locus_tag="VWZ30_000838"
                     /pseudo
     CDS             complement(25168..25428)
                     /locus_tag="VWZ30_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(25431..25931)
                     /locus_tag="VWZ30_000839"
     CDS             complement(25431..25931)
                     /locus_tag="VWZ30_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9334612.1"
                     /translation="MIDQLSKYYSCRILTEKNIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFIGFWNGSDLVAVMDFVYAHPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKACLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIV
                     EQSLED"
     gene            25967..26146
                     /locus_tag="VWZ30_000840"
     CDS             25967..26146
                     /locus_tag="VWZ30_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334613.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQQYI
                     LLQANFLKISRVQLQ"
     gene            complement(26124..27092)
                     /locus_tag="VWZ30_000841"
     CDS             complement(26124..27092)
                     /locus_tag="VWZ30_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU9334614.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMALVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVILAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(27178..27393)
                     /locus_tag="VWZ30_000842"
     CDS             complement(27178..27393)
                     /locus_tag="VWZ30_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEU9334615.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            complement(27402..28256)
                     /gene="cvfB"
                     /locus_tag="VWZ30_000843"
     CDS             complement(27402..28256)
                     /gene="cvfB"
                     /locus_tag="VWZ30_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEU9334616.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            complement(28316..28873)
                     /gene="frr"
                     /locus_tag="VWZ30_000844"
     CDS             complement(28316..28873)
                     /gene="frr"
                     /locus_tag="VWZ30_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU9334617.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLII
                     PVLTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            complement(28894..29619)
                     /locus_tag="VWZ30_000845"
     CDS             complement(28894..29619)
                     /locus_tag="VWZ30_000845"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU9334618.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRSAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQPGNIKRVVFGENIGTTVSNNI"
     gene            complement(29703..31037)
                     /gene="trmFO"
                     /locus_tag="VWZ30_000846"
     CDS             complement(29703..31037)
                     /gene="trmFO"
                     /locus_tag="VWZ30_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU9334619.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESATSGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            complement(31381..>31945)
                     /locus_tag="VWZ30_000847"
     CDS             complement(31381..>31945)
                     /locus_tag="VWZ30_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU9334620.1"
                     /translation="NNDGAKFVNKAKQALKKIEDASEEKLTEVYRQLFGRKFAQSTST
                     TNRASNEEFIDELFNVNIEYNIVLECEITQDGFRKALLSDFLKSRFKIKNRKSLDFEI
                     IKSDIPDNLLSDVKYYWKVRNIGAEAIRRNCERGAIFSGKIKHHEDSQFKGDHYVECY
                     AVLDNTVIARDRITVPIDPLCGKDFIE"
BASE COUNT         9108 a         7185 c         5424 g        10228 t
ORIGIN      
        1 cagaaagcaa cgtgaccata gattggcgat gccccagcta caccattcat gaatacagct
       61 gcagaacctg gtttaggagt cgttgaaatg tcatatcctt gcgcttggcc actatcaacc
      121 cactgattgg catttcctag atagggatga ataccaactc caatttgagc aaagcgatta
      181 tagacatacc aagtacaatt ccctagttca taagcgttac ctgaatagcc ttgtccttct
      241 aaaagtgctt gatcggtagg tagtccataa ggtagcttat ctttatattc tgctggaact
      301 tgcttgctag aaacacctgt cgaacctcct cctttatttt ttaagaaatt caaccattga
      361 cgagctgcat tttgtcgttc taagagctta ttacctggta ctcccaacca agcgttcaag
      421 aagtcctctg caagagcttc tacggaaccc gtcgatgtca caattcttct gaatgctgca
      481 cgtctagttg gatcatcttc ctccagaaga aattttgttt ggacttctgc agaccaaata
      541 tctccattca ctgattttgc aaaattccac aatgcaagag cacgagggcc agtaaattga
      601 ccaatcccaa tcccgatata gtgcaaacca ttgactagat accctctttc atttagaggc
      661 aaagtagggt acatagcttg aaaggctccc cagttgccaa ctaaattttc tgcagttggt
      721 tctttagcaa ctttatcaaa ctgattatta gttagatagt ctgtttcata gcgtttaaaa
      781 gtgatggaac tctcaatatc aaatcctccc aaaacggctg caatcccttc atcagttgcc
      841 tccggtatat tctttttaat cgcatccgca aaagctcttg cacgtcttcc tttctctcca
      901 gatgtttcaa tattactgac aacagacgtt gcttgagtgt actctacacg ctggaagtaa
      961 aaaccgacat tgacataagt ccaactcttt tcaacattac catctttgtc tttcttattt
     1021 aatcgatcag gttctagctt ttgataataa acggtttgat ttccttcagg tgctaccttt
     1081 ccaataatat ctccagcttt gacgtgatca cctgttttat agcgaatcgt atctacgttc
     1141 ttatacagaa acttggaatc ctgatcacta atttctatgt cactcccctt aacttcaact
     1201 ttcccatcta aaacagctaa cagagactga cctcctgaaa cctgcaaaac agatccatta
     1261 aaaacttccg tcttcgattt atatccaaaa cgctcaataa tctgaagagg tgcctgcgat
     1321 tcaggttgtt cttctgtata aaatggatta tctagttctt gaagcaacat atattttcca
     1381 ctatctctag caatttctaa gagctcttta tactcctcta actcgtcctt atcaagctta
     1441 tagagattat ttttggtata aagctcttcc atcgttttaa gattgtcttt atccccattc
     1501 aaattcttcc ataaggtatc taagtaaagt tttccacgat tctgttgtga aaaatagtta
     1561 ttcccgtcta tacgaagctt atctgaaata tcatcatact tataattcaa gtataaaagc
     1621 ggatcgtcaa tttttgtcca ataatctacc ttgtcattcg atttttcacg atccaatttt
     1681 gaaagataca accaggtatc attcatatca aattcatctt gacgggtaat tcctgaacta
     1741 ccgataccaa gtatagccag aaacaaaagc agaccaaatg caatcagata agcctttaag
     1801 ctaaatggat ttttgataat aactttaagc ggtttagaac ctacacggta aaacttccta
     1861 gccttcttag ccactttccc tgttttagaa gtagccaagc gtttcttata atcctgaata
     1921 cgacgagaga gttttccttc ccaagaaaat tctcttggag tttgagtgaa gcccctaccc
     1981 ttagccttat tgtagactct atttccaagg ctatagctcg atttaaccat tccttggcca
     2041 agtttaccgc tatacttccc aatcgttttg attcgtttcc cttgaatctg aatatcctga
     2101 taccgatatt tagttcgtgc tatatctgat agtgtatcat cctgatgcgc aacagagcta
     2161 gcctgttgat tagcaactcg ttctacagat tgttttgccg cacgaagaaa cttcttcttg
     2221 cgtgaaccac cattagcctc ttgagcgact ttaaaagttc ttttagaagt ttttttctcc
     2281 agtttcgcct tatctaattc ctgcttaagg aagacccgct ttttattgtt cctatctaat
     2341 tcctgcttaa ggaagacctg ctttttaatt tcctctggac gaagtgactt tccttctttt
     2401 ccaagatttc ttccttgttt ctggatattc ttgtactctt tttggattct cttatactct
     2461 ttttgtattt tcttgacctt gttcttagaa gctcgaaatt ggcttcgact cttctttacc
     2521 aactgacgtt tagagtgagt ctgagattgt aacttcgtaa gttgttttgc gctatcttgt
     2581 tgaaccgttc ggtaattctt acgaactttt tctagctttc gatctaacct atgaccgatt
     2641 gtttttggtc tgcctttctc ctgatccata gtgagacccc taagcgtctg ttgcgactaa
     2701 gttatacaac ttagtattct ttggtattgg attttcaaat ggcactacaa ctggcccagc
     2761 tacgattaag ccagtacctt tagcttttgg ccgtgctaag tagcgaatct gtttatcagt
     2821 cagattgaca atctctttta ggatagccaa atcttggggc ttttgtttga gcaaaatcat
     2881 aaactcacta tttgagagca atttccgacc ttcttcaaca gcagccagcg tttctacgtt
     2941 ctgggtaatt cctgttggaa tcgcaccata tttccgaatc cgtgaatata acgtagtaaa
     3001 gaagtctgct tgagccttat ttttaaatag aatctgcatc tcatcaaagt aaacccaagt
     3061 agtacatttt ccttgatttt ctacaatttg attccagata taatcctgaa tcaccatcaa
     3121 agcgaacggc ttcaatttgt gcgacaactt cttcaggtta aagataacaa agcgatgatt
     3181 aagatccacg ttggtattgt gagcaaaaat atcctgagaa ccagttgtat aggtctctac
     3241 tgctaaagct aagtcttgtg cttcaggttc gggttgttct tttaagacct ggtgccactc
     3301 ttttaatgtt ggggtaaaat tctccgtcaa atagcgttca taagtcaccc ctgttacccg
     3361 gtcaataatc ccaatctggg catctgtcac ctctgacaga atcgattcaa acaatcccat
     3421 taataagttt gccttatctc caatcggatc gtcatcctca tcgtccaaac gatctagatc
     3481 tggtagatct aagagattga tatgtgtttt agatccaatc gaaatatcca ccatctgagc
     3541 accgaaagca cggccaatat cagaatattc atcttcggga tccacgatga tcactcggtc
     3601 ttctggatat ttcagtaaag ttggaataac ttctccaccc tttattgttg tagatttacc
     3661 agatcctgaa gaacctaaca caactcctga acccgtgttc aaatcacgct ttctatctag
     3721 tgtaatgata ttgtttgata actgattttg accatagtag cgagcgagtg agctttcaga
     3781 cttcaagtcc acgttggtaa aaggaacttg cgtagcaata ttagctgtcg tcatctttct
     3841 cataaaattc ttcttcacat tcaagaagca ctctccaata ggtaaaaccg tatttagagc
     3901 ctcttcttgg taataataga gatcttcaaa ctctacccct agcttccgtc cagcacgctt
     3961 aatcttctgc tcattccgtt gtagttcctc ctggttatcc gccttaaaat acactgcaat
     4021 caaaccagaa aagattttct ggtcatactc agtaatctcg tctcgccact tcttggtagc
     4081 ttctgagatt tcttggtcac gaccagcaac gaccgcatca tcaaagatac cagattgagc
     4141 cgccttacgt ttcgaacgta acttcccaat ttcagcatct gtctctgcat cgtcaatctt
     4201 ctctaagatt tcaacgttct catagggttc tgcatggata gtaatagcca gttccagccc
     4261 cagtgtcgtc aaatctcgaa taagccgatc agacatccag ttaggatagc gacgtgcata
     4321 gagcaccttg caccaacgat catctataat catgcgtttt tcttgaaatt caatcctgtt
     4381 tggcgcaata aatgatttag tgaataaacc tgtcaaagca atttctttgt aatcaaaatt
     4441 gacatacggg ttatctcgta acagttcaga aaagaccttt aggcgatcca aaccattcat
     4501 atccctgaaa gtaatatcag attcctgaaa ttgagttcct aaggaaactc caatatcttg
     4561 taggatcaac tgcgcctgct ttcggtcatc cgcattcgtt gaaatggtca catacttatg
     4621 tacctcgaag ttgttagcat gtgtcgtaaa tcgctccttg atcatctcat ttagctcctc
     4681 acgatagtcg tcaaaaccat catttgtttc ttcatacaag atattctgca aggagttatc
     4741 cgatactcga cggttaatga ctaagagctg gtagttacta ccctcatcca gtgaattaag
     4801 agcatccatc gttgtatcaa tgatactctc tttttcatct tgttgagcag ttgagtacga
     4861 agcatccccc aacataaagc tccgagaata cttctcacgt gcaatgtgca tcaatccgtc
     4921 ttcatggagc agtgtataat tgatggtgtt ttgtgtcgta ggtttcatct ggcgacgcaa
     4981 gcgatcaatt tttttctttt ctgctttttc tagtttttct cttttgcttt tgaataaact
     5041 catctttcgt atacattcct acctttccaa attctatctc ataggttcgt tgttggattc
     5101 ggaaaaagaa attgattttt tctttaatcc gcattcgctt agtcattccc gtaccgtaca
     5161 tccctccagg aacgacaaat agagcatccc aaagatagat gtaccaatca ggtagaccaa
     5221 agaagtaact agcagttgtc cctgagacag ctattatcaa acacaggtat agaacaagca
     5281 ttctactagt taagccaaat agaataggct tgtcctcagt ttcaaattct cttaaaaatt
     5341 ctgatccaat ttttttcaat tagtttcctt cccatcaaaa aaaaacagtc cgtttagaac
     5401 tgttagtctt atgtcattta tttatcacgt tcctttacaa gcatagctac agcaatctca
     5461 gattcctacc ccttcagtat cgaataggct tctgttagcc gttttttgtt cctgaaaata
     5521 gcgttcaaag ttttcatctc ctttgaagac cattttacca cgatccatat atttaatcca
     5581 ctgttccaaa cggtaaacat actctgtttg actgatacta tcagctatag cacagtacgt
     5641 tatatcttcc tgaagaaggg taagaattct catgccataa aaagcatctt gcttagcttc
     5701 tgcatagcgc caagcataaa tatatagttc gtcaccataa ctactcatcc gaacggcacg
     5761 aggaatctca gatatcttaa ctttaccatc caatatccac tccaataaag gctccgataa
     5821 taattcatgt tcataggcaa accaagaaag acgtgttatt tcttgttcaa gagaagttat
     5881 tttatccttc aactctcttg tcccatgttt cttttgtaaa tcattaaatt gtttatttcc
     5941 tatctccttt aacgtttcca ttgatagtca cctctttcta tagaaaacat ccacacgatt
     6001 ctctaaacat aggtaacatc gattaattct aatttttcta cacatagatt acactcctaa
     6061 aagctgcttg gcttttcgac tagatccaac cattacaaag atatagataa cccccttggc
     6121 aattgaagca aaggctgttc cccaggaatc actggaacca gtcgtaacct ttaggatatc
     6181 tgctgcaaca atcgaatcat aaacaacaga aacaataatt aggatgacac caataagggc
     6241 agaggctgca aagtatttca ggaagttaat cgtaacagat cgcaacgaat ccatcataaa
     6301 gaaggctact aacaatggag ctaaagcctt aagcatatat aaatccaagt aacgtaagaa
     6361 aacaataatc cctaaaataa cgttagctac tccacttaca gctttcccta cgaaagtaat
     6421 gatagttttt tcaactattc ctttcccacc agaaatatta gaatcaatgg ttgctttaac
     6481 agtaggtgtt acatgagcaa ttacctttac aataatatta agtaattcca gaatggcatc
     6541 gcaaatttga gatgaggcag aaatcatgat gaaggcaaat aaatacttca tcaaggcctc
     6601 tagccaaatc ttttgggtta gcgaacctcc tctggactta accatttgtg accacgatgc
     6661 catttcaaac cagaaaaaaa gacctaaaag gatataccca ataggtctga ctgcatcagc
     6721 catcatattt acagcttgat ttgtagcaga gccataacta gaaagagagc ttgttagctc
     6781 tctaatatct aaattcatag gctactccta aaattttagc tgtgataatg ctgcaacgac
     6841 tgcggccgca atccctgcag aagcaacagc caagaacgca ccattaatca tagaatcaga
     6901 cccatcatct tgtctttgct tatctttatt ctttcttcct tgaatataat caaccgcacc
     6961 attccagatc cagataagac ctgtgattgt tccaactaca cctagaccag taataatttt
     7021 tgtaatgatt tccattttgt tttacctctt ttaatttcat ttttttataa caaaaggcac
     7081 tgcagagatt tctacagtgc caaacacaat actattttta taattcttca aattcttcac
     7141 gataaatcgt ccgaacagta gctactagaa tactgaggcc aataataatt ggaacttcaa
     7201 aattcggata tgggaaaaat aaccctcctc cttttggtgc atcaaccact tgaccagcag
     7261 taataagtga atcatgggac attcctaaaa tatgcggtgt ataccatcca aaaatattat
     7321 caattatgtt ttcgcttggg aaattgaata gatgatgaat gattgccatt cccccaatca
     7381 atacgattgt tatacatatt ttagctataa cccacagaat aaacatacca atagtggatt
     7441 ttacaacatt aatacaccaa agaacgatat aaaagggagc tgtaaaaatt ctaatgatag
     7501 ctacccataa ccaggaaaaa tcacttccaa atttcataat cctactcctc cacgatttac
     7561 cgttattata ccataaaaat agcttatttt aaagattcct agagtattct aagcctcttt
     7621 tttcttgcgc ttgctgtaga gcaatcctga agcagcaccc cccattgcca gaccagctaa
     7681 cagcaattct gcagaagcct gctcacctgt agatggcagg cttcgttctg gttgttgcgg
     7741 atgttcttca ggagttttgg gctcttctgg agtctgagtc acaaccgtat tggaagcaac
     7801 ttccacaccg ttaactgtat gatagtaagt attttctact gctcctgact gaatgcgtgt
     7861 catttgtaga tatacttctg cttgaaactc tgatgtttct aaaatcgact ttaagagagc
     7921 atcatcaaag ctaatatcta ctttaccttt agccttgtct accacttgac ttgtgaactt
     7981 agttaattca tcaccagctt tgaatttttg gccatcactt gtttcaaaat ctacagcagc
     8041 aaataccttg taaacactct gataatcatc atgtgtttca tcgtagtcat cgctaaattt
     8101 atactcaaat aactgttctg agcgatcttt tggcactagg gaaccaacta atttgtaatt
     8161 aaatgtttga ttcagtttga cctcttttcc atccaagctt tctttgctta gatggtcgat
     8221 cacaacatct ttcttcggtt cgatctttgg tacattattg actactgttt cagttacata
     8281 atccatccca aaatcaattt gataggctgt attttcatac ttaccacctg tttgacccaa
     8341 ttccttctta acagtcattg gattggtaat ggtcagaact tctccagctt gcacgtaggt
     8401 cttgtagaat tcctctggat tctctgcctc gaatacttga atcgctcctt tcggagtgaa
     8461 atggctagac ttcagggctt cttggacacc tgatggtgcc ttagctagac tctcataaag
     8521 agacattttc aaatctttga ctacttgacc tttagaatcc gttacttgaa caccgtcttg
     8581 attgatggta acagcttctt ctggataatc atcaacgata tagaaccctt taccaatacg
     8641 gtctttatct gcttcaatgc ccttgtattg gctataatct gccgttacct tgtagtagtt
     8701 gacagagtct gccaatacct gtttcccatc gatgttcacg cctgctttat tcaaattctt
     8761 cttggtaggt ttaacagtag gaacattatt gacaactgta tctgtaatat aagctgaacc
     8821 aaaatcaagc tgataagctg tattctcata cttaccacct gtttgaccca attccttctt
     8881 gacagtcatt ggattggtaa tggtcagaac ttctccagct tgcacgtagg tcttgtagaa
     8941 ctcctctgga ttctctgcct cgaatacttg aatcgctcct ttcggagtga aatggctaga
     9001 cttcagggct tcttggacac ctgatggtgc cttatccaga ctatcataaa gagccatttt
     9061 caaaccttta actacttgac ctttagaatc cgttacttga acaccatctt gattgatggt
     9121 aacagcttct tctggataat catcaacgat atagaaccct ttgccaatac ggtctttatc
     9181 tgcttcaatg cccttgtatt ggctataatc tgccgttacc ttgtagtagt tgacagagcc
     9241 tgctaagacc tgtttcccat cgatgttcac acctgcttta ttcagatttt tcttggtagg
     9301 tttcgcagta ggaacattgt tctctacctt atccgtctgg tagccactac caaagtctaa
     9361 ttgataagct gtattctcat acttaccacc tgtttgaccc aattccttct taacagtcat
     9421 tgggttggta atggtcagaa cttctccagc ttgcacataa gtcttgtaga actcctctgg
     9481 attctctgcc tcgaatactt gaatcgctcc tttcggagtg aaatggctag actccaagga
     9541 ttcttgtaca cctgatggtg ccttatccag actatcataa agagccattt tcaaaccttt
     9601 gaccacttgc cctttagaat ccgtcacttg aacaccgtct tgattgatgg taacagcttc
     9661 ttctggataa tcatcaacga tatagaaccc tttgccaata cggtctttat ccgcttcaat
     9721 gcccttgtat tgactataat ctgccgttac cttgtagtag ttgacagagc ctgccaagac
     9781 ctgtttccca tcgatgttca cacctgcttt gttcaaattc tttttggttg gtttaacagt
     9841 aggaacatta ttgacaactg tatcagtttg atatccgtta ccaaaatcaa cttggtaagc
     9901 cgtattctca aatgattttc ctgtatttcg cagttcttcc ttagttacca ttggatcaat
     9961 aattgtaacc gattttcctg atttaatata ctggttaaaa taagcttctg gatcatccac
    10021 taaaaatact tggaaagcac ccttgggctt aatttcagaa ctagcaagaa aagctttaac
    10081 cttatcatta tcaacggcat ctatagaatc atactgatac accgagatac cagcaacttc
    10141 ttttccttcg ctgtcaatat aacgaatgtc cgattgatta atcgtaacag cctcttctgg
    10201 gtaatcatca attgctccga atccttttaa aatagtagat gaatctgcct tcatcccctt
    10261 atattgatca taatccaagg taatatggta gtaatttgtc gaaccaacta gaactggttt
    10321 accatcaatg ataactttat ccttattgta attatgttta agaggttgaa tgaaattatt
    10381 attcggattg tccggatcat tcggtttacc aggtgttgaa atctgaacaa tgtttgaata
    10441 acttgcgtac ttattgttaa tctttaattg gaagttattc ttgtaggtcg ctccatcatt
    10501 cgttacttta ccaactaaaa ttggagctgg tacttgtact tccttactta aatcttgatt
    10561 aagcttagat tttaaacttt ctaagccttt catccgaaca acatggctcg actcgtcata
    10621 agttaattca aaatctgagc taattttttt ggaagcttca acatctaaca caaaacctga
    10681 aggcaaggca tccacaattt caaagtctgt aatagcttca cgattagctg gaagaggctt
    10741 agttgttagt tcccactgta ctgttgaaag tttaggtaca tagcttttat caatagattc
    10801 accagaagaa ttttttacaa acttttctag gtcaggcatt gcctgtaagc gtgcatatct
    10861 aacccttact gttggagggg atactaaaga ggggatctct ttttccttta tctcctcagg
    10921 tttgggtggg agtacctctt caggttttcc agttggagaa ttgggtggag tagtttcaac
    10981 ttcataagtt ggtttgcgag gtggatttac tggtggaatg cttttcaact ccgccccctc
    11041 cccaagaatt gtgaagacaa cgttttgaac atttccactc aaattctctg catcccattc
    11101 agcgggagtt cttccctctg tcgaaccagg agatgatgtg tgaaggtacg ttaaactaga
    11161 accataccct actgaagcaa aggaagcctt aggaatatct gaggtatcat taactccatt
    11221 ttctactccc ttcataacgc gaccatcaaa atccaaacct gaaccacttg gattaagaac
    11281 cattccacga ccagatgagc cataactcaa atttgaacct tgaccccaat ctacatcggt
    11341 aataactgtt ccaattaaga gggaaacagg agttgtcgta ccttcaatat aaaaatcaaa
    11401 aaccatttcc aaacgggtat gattattata gacaaagtag aaacttccac catctccacc
    11461 cttgaggtag atatctgaat ctgtcacagg aacaccattt cgaacttctc cctgatagtc
    11521 tcctaaatcc gaaaccatga cttttgccat gatctttcta ccatctgtca acgttccaat
    11581 attatgaata taaaatgtat ctccaaatgt tggagattta atgatatcat aagtttccgt
    11641 actatcctta ctaactcgag ttcctgcgcc tcctgttaca taagtatctg catgataacc
    11701 taaaaaacca cgcatactct ctactggtag attatcatct ggtactactg taaaatcatt
    11761 ataatagtct aagcttcccc gaccagctga attatatcct ccataaactt tgaaacctgt
    11821 ttcagatcca aataaaggct tacttgtttg aattgcatct cgctcagctt taatagttgc
    11881 tagcttcttt gtatagtctg tcaaagagct ttcataagct cctctcttag cttcatttcg
    11941 ttttcggatt tctgcattct cttgattgat acgatctatt tctgcctgat tctctcgttg
    12001 atttttttca ttctctgcac gaatactagc attcttctga ttaattcgat tcacttcagc
    12061 ttgataagaa gttaaatcct tttgatattg ttccttcttc gcagtatttt catcttggat
    12121 tttctttcgg ttcgtttgat tttgtctttg atcttcacga tatttttcag tagctttctc
    12181 aatactatct gcttgtgtgc aataatcact ctcgacttcc ttttgctttt ttccaactca
    12241 gaggctgtac ttgcagttcc tttatctact acagaatcaa attccacctt caatccagct
    12301 tcttgtgcct ttcgaatagt ttcatctaaa ctggaatgag aaatagaaac ttctaacgtt
    12361 cctgtttttt cttcactctt tccttgattt tcagtagaaa tactgcttgg actcgtctgg
    12421 gcataaggcg catttgtagc cttattttca gttcgttcat ctgctataac tgggcttatc
    12481 atgcttaaag ctgctaaacc tagtatcact ccacaagttc cataaatttt tgatttccga
    12541 atagatccat atgtttttgt ttctcttgct ttgtgcattc tatctcctta tctaaaatat
    12601 caatcaatat aaaacctcaa atgttggctc tcgaggtctc ttataatata aaatcatctc
    12661 tttcctttcc aatagaaaaa agccagcttt cactgacttt tctatacaat attgtaatga
    12721 attttaagaa atccacttac cagaataatc taaataataa gcaccaatcg tctcattttg
    12781 tgccatctta ccatctgctt tcaaataata ccaagcactt ccgtccttga accattgatc
    12841 tctcagataa cttccatctg atttgagata gtaccaagct ttataactgg aatcatatag
    12901 ccactcactt tgtgccattt ttccatctga cttaaggtag taacttcctt gccaactatt
    12961 ttgtgcatag cttccatctg atttgagata gtaccaagct ttatagctgg aatcgtatag
    13021 ccactcactt tgtgccattt ttccatctga cttaaggtag taacttcctt gccagctatt
    13081 ttgtgcatag cttccatctg acttgagata ataccaagct ttatagctgg aatcgtatag
    13141 ccactcactt tgtgccattt tcccatctga cttaaggtag tagcttcctt gccagctatt
    13201 ttgtgcatag cttccatctg acttgagata ataccaagct ttatagctgg aatcgtatag
    13261 ccactcactt tgtgccattt ttccatctga cttaaggtag taacttcctt gccaactatt
    13321 ttgtgcatag cttccatctg atttgaaata ataccaagct ttatagtttg tatcataaac
    13381 ccattcatta cgtgccatca caccatttga tttgagataa tagtttccct tccaactatt
    13441 aacaagagct ttccctgcac gatcaaaata ataccaataa cttccctctt gtttccattg
    13501 gtcacgcaaa tcagatgtgt tttcaatcga tttagtaggc tcagctgagc cagcgcttgg
    13561 tttacttggt tctgtcacag tagctgaacc ttggttagac tgcccttgag atcctttact
    13621 atctgcttgt ttttgaaaat cttgataaat tttttccttg gctttgtagt cttcctctag
    13681 ctttttgatt gcttgacggc gattgacact agttggatca ctcacagcag ccgtgctaaa
    13741 gcttgatttg cttgcacgct tgatatcttc ttgggaaacc gtgataaaat gggctgaact
    13801 aacagaagaa cgactagaga atgaaagccc aaaataatct tttgagttca catttcccca
    13861 gttcaagcca gcaatactct gagcatgcat ccactcagta tcattgaaca taaactctat
    13921 catcccaata tagatagcct cttttaactg agccttgtta gctgttttga aaccaggtag
    13981 acgagaagct agattttcat agttttgctc cccttttttc tcttcttcag aattggttgt
    14041 aggcagtcca tattctctcg ccacatgatt gattccagtc gcatcatgac caagtgtacc
    14101 agatacgctc attttatgcc aaccccagtt atcttgaaca tatgctttcg ccaccttatc
    14161 tgcaaagtca agtgccgaaa ttgaaacact gactctaggt gtcccaacct gagaacgaac
    14221 ttggttaatt aaatcttggg caaaaaatga caactcctga cgaacagcta gcggtagatt
    14281 gttgacatcc agacgttctc cctcatcagc aacatctttg tggaaactat tttgattctt
    14341 taatctcaaa ctttctgact gcaaaataga tttcgcactg tcacgttcag tctggctaat
    14401 attaaaatca gaaaaagccg tcttcaaagc ctttatatac ttatcactca gtataatcgt
    14461 attgctaggt gaagatgata aagcattgtc atagatactt ttagcttgtt catattctct
    14521 ctttgcttta ataaattcac tttttaagct ctctacttga ctactcgttg tactatttgc
    14581 atacacaggt tgactatttc ctaacccaag caacgttacg ctagcagttg ttgccaagat
    14641 ggttgttctc aatttcataa tcttatctcc tttttgttta aaaacgttct taaaatattt
    14701 attttatact tttattttac catttttaga ggttgttctc aatttttttc ttaattttca
    14761 ttcatctatc cgcttaatca aagataggac gtttaaaaca gttcaaatct cctctccttg
    14821 atgaaacaga tagatgaggc tctatctgtt taatgcttcg ttggacgatg atatccttta
    14881 ccagtcagca gtaaacgatt ggtatttcct tgcttaatgc gtctaaccac taaaacatca
    14941 cctcgtttga tggtgcgaat ttctttataa atttttgaat attctacaat catccaaact
    15001 gtttctagtt tacctgctaa atatcctttt ttaggtacca aaccatatct gctggttaat
    15061 ttgatttcat cgccattatc aagtcttgcg gttgcatttc cttgatttcc taagcgtcta
    15121 aggtcagcta aagtgacctc tgtaactaat tttgattgat tcatcttatc tcctccttac
    15181 tgaatcaagg ctgatttaca gccatgccga ggtatgacgt acatacctgc tgtatttttt
    15241 acacacggtt ttaagtctag aaaccgcaaa ctccttcagc ttaggctttg cctgctttta
    15301 cgtggtggaa cagcactaga tatccttttc tagcctacac accgcccttg ttcttctttt
    15361 tatgcttagt tccgctaatc aggacagtgg ggatattcaa ttttcaaagg tcaaaagtag
    15421 ttttatgact tgctcaggtc aaaaaagcta gatttagtct catgtcatct atagcttagt
    15481 tactcagata cctatactct gcttctctat gagcttgcat cttggcctcg ctcttacgtt
    15541 cgccaatcaa attttcaata gattgaatca agtaagcaaa aggactatca cattcaggaa
    15601 tccgttcgat acagtgaaga acctcactac ttgttatcca tcctgtctga atgtattggc
    15661 caacaatcat cttttggcgg tgagtcatat tataatgctg gtaccacggt aaatgatctc
    15721 ggtagctatc tgcaatttcc tgcaagagag aatagacttg aggtttgata aatttcttcc
    15781 cacttgtttt ttgagaagga gtgtcacaag ttccagcagt ctgatccaat tccttagaat
    15841 tggaagcagg ctttctagaa ctatgtctag tactattatt ctcagtatga ctcttctcag
    15901 tcttaatagg gttaaaattt tttacctccg gaggtaaaga tttttgactt ccagaattta
    15961 ttgatttgat aggcttttct aaaacagagt tttcatcttt caaaatagaa gattcttctt
    16021 cactgcctgg acgttcaatt tcaaataaga cacaaccttt gtaatctaaa gtttttacat
    16081 ggtggtagac tacagttcct ttgtcacgtc ctttttctac gacttcagca tcatagtgtt
    16141 catattcttg taattcagca ttcacatttt gcagatacaa acgattcgga cgattcttcc
    16201 cttgtttcac ttcacgaagc aagccaagct ctttcaattc tttcttgatt ttcaacacag
    16261 ttggcttact acgatctaaa atttccatta agtcctcaac cgtatagaca agaaatactg
    16321 aaccattctc atcaacataa gtcagttcac cgtttctgta agaataaata ctcaacatac
    16381 atcgatcata gagtacacca taagcaattt tgccatcatt tgataatttc ttggcataga
    16441 gactcgtcat cttgattttc ccagtaggtt tcccttcagc agaaaatttc ttcttaacat
    16501 ttaccatgaa aatttgtggg atttgataga aggtctgtgt tgccacttga tttactgtaa
    16561 tatcagccat gttatcacac ccccatcatc acagtaccat ttcccataga gaacagaact
    16621 ccttctctct ctaattcttc aaccgcctta acagcacgtt ttttactcat tcctgttttt
    16681 ctcatcactt cgttaacagc tgcttttttt gaaaattgca caaacatttc tttagtcatt
    16741 ctactatctc ctttttctgt ctcactaatt tgagctcctt tgaaaagcaa ctcggacatt
    16801 cagatagacc aagctgaaaa tccaaatggt caaataaaaa gttacatcgc tcacactgca
    16861 ctgtgcttct aagtattgtt tcacctaacc aatctctttc tacaacatct aatacatctc
    16921 tacattcagg acaatgaata ggcttgaaaa tattatcttc ttcccatttt tcctcataat
    16981 cacaatcaaa acaaacgatt gtttccaacc acatggatta actcccttcc aatgttcctt
    17041 ctaattcgcc tatcttgaac ttaaattcct tgattttctt tctcacaaga tcacgttctg
    17101 cactcattag ttgtacctca cttttttgta aggttttctt aagacttcta gtcttgcctc
    17161 atatagctcc agtgaacgct tataataatt caacatttct ctttctagtc ttgtcatgct
    17221 atctcggcct ctctcgtttc aaacagaagc aagtaactca ggagtaaatt ccatcgtcgt
    17281 ctacaggcct tcatcataag tcagttggct ggtttgtccc cattccccat gttccatctc
    17341 tagctcttct gaaatacgga taagtgccaa gcttaattct aagatataat tcatgaataa
    17401 catcatcttt tatgactttc cataaaatac tagcgagctt ataataatcg tttccaagcc
    17461 aaactctagc aaggtcattg tctggagata cttttcatca gaatttcctt cagaaataca
    17521 atcaaccata ttttcattct tatcaagatc tattacatcg aaaatttcta gcgaatcaat
    17581 atccataaca atttcaatat tatctgacat tttttctcct taatttttgg caacgaaaaa
    17641 agagaacgga ttttttcgtt ctctacattt atttatattc tttctgcatt tctcaaaact
    17701 ctagacactt acagttattt ttttgaaaag tttctgtttg tattttggtt tggttttcga
    17761 atcaaaaaga gagaatcaaa ttgattccct ctctatgtta gtttttactt attttcccta
    17821 taggaaagct aagacctatt aaaaacttcc tttttacgtg aacttcccca gctttcgata
    17881 aatagaatcc ccactaacaa aaggataatc aggcaaggaa gtaagaccat ccccatgctc
    17941 caatttagat cgacattatc aatctgctta gataatcttc cagataaaat ctccactgaa
    18001 cgcaagtaag taaagggaat cagatgtgca atcctttgaa gaggctgaat ggtttggatg
    18061 ccaaacaata agccaacaat cccaatgagt gaaagaaaga ggacaggcat tttttgcttg
    18121 aaaaagtaag caatcaagta cacaacttcc acaatgacga taaaggctaa gaaagctaag
    18181 agcaagccag gaaataatac atcttgtatt ttcccaatag ttacctcttg attcactaag
    18241 ctataaatcg ggtagggata atctaactgt ccaaaaccac ttatcagact tcccactaga
    18301 aaagaaaagc cacagattcc gataaacagc acagttacat atcccactcc aactccaaga
    18361 gaggatattg caaatgtcac ttttgaaaca ggatataagt gagctgtgtc cagatgattt
    18421 tgatatcttt ctgcaaatag ttgtgttagc ataaaaataa tcgcaaccac aaacaagctt
    18481 gggatgataa cctctaaaat ccagacaatc tgatcaatcc cgtgggtcgg aaactccaaa
    18541 gtatgtgctt ttatgttcaa gggatacagg gcttggtaaa tcttccgttc gcggtcaacc
    18601 cccattttta agccagagct agcagtcggg tcatttgata caaattcata attcttctct
    18661 tcatcttgcc actgcagata ataggcctct ttccagcgcc cttcttttaa taaagtcaga
    18721 atttctgtct ttcgcgtcaa aagatttttt tgcaagtcta aattattttt agcaaactgg
    18781 tattcctccg agctggtgtc agacatttgg gaaagtttcg cttcattttc attgatggct
    18841 ctcccgttat ccacaatgcg actttccaac ctactctcca agctgacgga gtttgcagtc
    18901 tgactattta aataaaaggt aacaccaagt acagatgcaa ataaaagtaa gacaatccag
    18961 tttaagcggc ttttgaaaac ttttttcaat aaaaatagac taacatcttt cataacggaa
    19021 cctcttctat ctgcccctga tgaatggtta ctactctatc gcagacatca accaactctt
    19081 ccttatagtg ggaacttaaa agaaccagct gctcttgtct atcgatttgt gcaagcctat
    19141 caaaaaactt ctgtcgataa tactcatcta agccatttgt aatctcatcc ataagccagc
    19201 atttggcctg actgaggaaa tacatggcaa tcaccaagcg ttgcttcatg cctaaggaat
    19261 acttgcgaat gggaaggctg atatagtcag acatttccca ataggcaatt tcatccctca
    19321 agttcaggtc tgacttccag atgtttttga taaggcgaag atagtctatc ccacttaagc
    19381 ttccatccag ccattcaacg ctctcataat aaaataaaga aggaggggct gcaatatttc
    19441 cgctacttat aggaattaaa ttgctaatgg tgcggaatag tgtcgtcttt ccagaaccat
    19501 tgatagcaag aataccataa atcctaccct tttcaaatgt aaaatcaaca tcttgtaaga
    19561 tgacttgtcg cgtttttaag gtcacatgag taagctgcaa catacctagc cctccttttt
    19621 atcactcttt aaggattaat agcctccgct gtagtagttt attacctcat aacgatcata
    19681 gttccagcct ccgccaactt tatactcaga atagctgtaa taacgagacc atgacggaat
    19741 atcactatat ctatgtctgt gataattcat actagactta tagaagatat tccaatcata
    19801 ccgataagtt ttaagaacgc tcacagcaga tacactggac tgaaaaagac caatagataa
    19861 caaactagct aataaaacaa cttttgctga tttttaatga ttttatatca tcaatatcga
    19921 agacgctttc attatatcgt ttttatcatt attatgcaac agatttttag tttattttac
    19981 taatatataa tatttgtcct tcctatcgaa cactttcgat tttccaactt ttccacccct
    20041 taaagcgtat agctccttgc tgatcagttc gataaacttt gctattgata gtttccagtc
    20101 gtgtcaatgt ttcctgatgg gggagtttcg tccgattact ctttccaact gagatgaggg
    20161 taatctctgg tttgagtttt tctaaaaacg ctgaacttga tgaggtttta gagccatgtt
    20221 ggctagcttt caaaacatcc acttctaggt caggatattg cctcagcaag tctctctctc
    20281 ctttctcctc caaatttcca gtgaagagaa attgcttatc caagagtttc ccatacagaa
    20341 ccagggtatc atcatgaccg ccatctccaa ttttccttgg ggataggact tctaactgac
    20401 ttccaaaaat tagaaggttc tctcccactg acacactacg tactttgttt tgagtcgcct
    20461 gtagttctac cacaaattcc ttctgtttca gactgccttt tgacactaaa atttcgccta
    20521 catggaaagc cttggtcacc tccaacaaat ctccaatatg ctccttgtcc gtatttgtca
    20581 aaatcagctg gtcaatcttg ctcactcctc gacttttaag atagggtatc aaggttcgct
    20641 gagcattgct ggtcgtcgcc ttttcttgcc atttttcgat tttcttctca gattctgcct
    20701 tgccaccaac atctatgaga atggttttcc cagttacatc ccgtaggaaa atactttccc
    20761 cttgcccaac atccagcatg gtgatttcat tttccagtgg atacttggtc aggaaaaaga
    20821 gacctgtaat caataagctc aacactgcta gccttttaat gtttttcctc aaatcataga
    20881 ctaaagccaa ggaaattaac aataaaatta aaagccacgc attaggttgt ccaaagacta
    20941 gaggcctact tgccacctgt gataccaagc gaatcatccc ctccaaccat tcaaaaataa
    21001 aattcagctg aatgactgga tagagaaagg aaagggcaaa taagatggac aagagcggta
    21061 agaagaccaa gtcaaataga aaggaaaaga caaaggtcaa aaggatagac caaggttgaa
    21121 attccgcaaa atagaaggat agaatgggca atatgcccaa ggagatgact agactttcac
    21181 tagcaacagc cttgagcccc tccccttctt tgctggtcat ggtcaggata aaagcataag
    21241 cgcaggacaa gactcctcct gctgtcaaga aaaagtttgg catgacaata aagaggacaa
    21301 gcaccgtcaa ggcaaaatta tccaagccct taaccccatg ttgagccagt agcttttgca
    21361 agagactgcg aataaccgat gctgaaaatc cagttagtcc cgcatagata agggaaaagg
    21421 gataagtcaa ccatttcaac ttttcttggg tcaagcccaa tcgcaagaga agtttcttaa
    21481 atccattcat gaaaaaacct acctgcatgc cagatagggc aaagaggtgg ataattccta
    21541 gactggaata aagctcattc atctcctcaa agtcggtgtc cagatgtccc agcaagagtc
    21601 ctgtcatgta attgcgcata gggtctggaa agtgcgtctt aatccaaacc acagcctttc
    21661 gacgtaaact ggacaagttt tctcctatat cccaactgcc aatcttttga agtgactgga
    21721 ttgttttgat attgagagtc tggtaaattc cctgagtctt cagataggct tggtaattaa
    21781 agccaccaaa atttctctgc ccttctggct ccgaaagctt cccttctagt cctatctcat
    21841 gcaggtcggt taaagcttga aaggcttctt tctcctcctc ggactggagt ttataataga
    21901 cttggaaagc acgaccatca gacttaccac gaaaggacag actgtcaccg ttgaccttaa
    21961 tagtgtctgg taaaatccgt accctttcaa cagaatccgc cagattttga ctcgcttgac
    22021 tctgttgcca agtttgaaac agaaaccaga agccaaagac tccacaaatc actagaactt
    22081 tacctgctga tttccaagga aattggataa agaggcagac tagcagaaaa acaaaaccca
    22141 acaaagcaaa ataggatact gagaaaatgg cgtagtaaag ccatagtaac agaaaactca
    22201 ggtaaattag gggaatagag aaattcttaa tccactgtaa catagtcttt aagtttttct
    22261 atggtcttgg caccaatgcc agagaccttc ttgagctcgt ctactgactt gaacttgcca
    22321 tttgcctcac gatggtcaat aatgtcctga gctcgttttc ctcccagtcc cttgacctgc
    22381 ttgagttctt ccagactggc cttgttgaga ttgaccttct tttccttgct tgttgaagaa
    22441 gctgtccccg aaccagtctg ttgactaact gcttcttctc ccttagtagg aacgtaaacc
    22501 agagcctcat cactaacttt ctgagctaga ttgagcgact tgctgtctgc ttgctctgtc
    22561 aagccaccag ccttctgaac agcatcattg actcgactac ctacaggcaa gtcataaatc
    22621 cctggcgatt tgacagcacc tttgacatct actgtgatta gatcttgttc aactggttct
    22681 tccttctctt ccttgttcac ttccttttcg gataccaaat ccttggaaac agctgcaact
    22741 tcagcctgca aattcgtttc ctttacaggt gtttgtggag ctggctttag caggaaaaat
    22801 ccgcctacaa gcaagcccag accagtacag atgacgatga ttttatactc tttgattttc
    22861 tcgataattg cttccatatt ttctcctctc ttagattatt cgtaagagga agaaaaaaca
    22921 gtcgaaaatt tcttttcaac tgcttattta tttgcaaaat agtcttcctt ggtcaagaca
    22981 taatgaactc ttgtcacgat tcggcctttt tcatgctggt ccatacaagc atatggttct
    23041 gcatgggaaa aacgcatgcc tgatttctcc atgacctttc ctgacgcagg attgtcctta
    23101 tcgtataaag caactaactt attcattcct atcttctcaa aagctagctc aatcacagca
    23161 cgattggttt ctggcgtcaa tccttgattc caatactttt tattgataat atagccaata
    23221 gctgccttct taagaacagg gtcaatcttg tgtaagtcaa tagttccgat aaacttgccg
    23281 ttatctttta attcaatccc ccaacgtccc aagggattag ccaagtagaa ctgagcaatg
    23341 ttattcttgg tttcttccaa gatttgattg gttggaaaag tgtaacgtgt attacccttg
    23401 tccgaggtat agtcaaacat tgcttccgca tcatccaaag ttacaggtct aagcaataaa
    23461 cgctccgttt ctatagacgg atactgggca aatttcacaa atattgattc catacttttc
    23521 cctccattag agcttgattt tcactagtct agcataaaaa aatttggatg acaagttttt
    23581 cttgattttt gaagcggttt catatacaat gaaaccgtca catttttgat ttatgaactg
    23641 gaatgtgaca accaaaacaa gaccgtcaat tgaaaggtag gttatctcaa tgttaatcgg
    23701 aatcccaaaa gaaattaaaa ataacgaaaa ccgtgtcgcc ctcacacctg caggtgttca
    23761 tagcttagtt agtcgtggtc atcgtgtcct tatcgaaaca aatgctggtc tcagttctgg
    23821 ctttactgat gctgactatc aaaagcaagg agctgagatt gtcgctactg ctggtgaagc
    23881 ttgggcagca gagttggttg tgaaagtaaa agaaccttta agttctgaat acggttactt
    23941 gcgcgacgat cttcttctct tcacctactt gcacatggcc gctgctccag aattagcaga
    24001 tgctatgtta gcagcaaaaa caacaggaat tgcctatgaa actgttcgtg acaatcaagg
    24061 acaactaccg ctcctcgttc ctatgagtga ggttgcaggt cgtatggctg ttcaaatcgg
    24121 agctcacttc cttactaagc aagctggtgg ctctggtgtt ctacctggtg gtgtaccagg
    24181 tgttccaaaa ggaaaagtaa ctatcatcgg tggtggtgtc gtcggtacac atgctgcccg
    24241 catcgccctt ggtcttggtg ctcaagtgac tattttagat attagtgcca agcgtctctc
    24301 agttctagaa gaagtctttg gaagtcaaat tcaaactctt atgtctaatt cattcaacat
    24361 tgaagcaagt gtgagagatg ctgatgtggt gattggagcc attctcatcc ctggtgcaaa
    24421 agcaccggaa ttggtgacag atgagatggt caaacaaatg cgtccaggct ctgtatcgtt
    24481 gacgttgctg ttgaccaagg tggcgttatc gaaacagctg accgtgtgac aacgcacgat
    24541 gaacccgtct atgaaaaaca cggtgttctc cactatgccg ttgccaatat ccctggtgcg
    24601 gttacccgca cttcaaccat cgccctaacc aatgtcactc ttccttatat cgaagctttg
    24661 gctggcaaag gattcgcaca agcaatctct gaagatgaag gcttgcgtca aggtgtgacc
    24721 acttatcaag gttacttgac tagcctacca gttgctcaag gacttaatcg tgactacact
    24781 gatatcaatg atttagtata aattttgaaa agaaaagggt gacttcacaa gtcctaccca
    24841 ctgtgctttg tgtcatcagc tattacatcg ttggtttatt gtactgtata ctttgaaact
    24901 ggagactttc gttccagact caattgtgca taagaaactc taaattgaat aaagtcaaca
    24961 gagtttcgcc caaccacttc gtcttgtaat gacaattata taaaccaaag gaagctgagc
    25021 tctttagtcc cagcttcttt ttatatataa aattttaccc gtgcaaagac agggccttag
    25081 cagacttctt ttttacttcg ttcacccttg ctttttcttt ttaggtttgg gcgttggcag
    25141 ttggttatac atagctaaaa tcaggtctta tagaaacatc ttattatcaa gttcttccac
    25201 tcaaatcatt tctttggcac ctttgtatgg aaactcaaaa gaagattggt caatcttatc
    25261 taagactgct tgcacgggtt taactaaaaa gcgatcgtca taaatgccgt caataatctt
    25321 gccgcggaag taaagaatat actcccccat catggaacgg taagtcacat catctaatcc
    25381 tgataattgt tccaaaacaa attccaaata gttcttactt gatgccattt ctaatcttct
    25441 aggctctgtt caacgataac aaccgtatag agttcttgct taacctcgca tccaattgat
    25501 ttaaagccct gcttttccca aaaatgctga gattgcggat ttcccttaac ataagccaaa
    25561 catgcctttc gaaagttctt agcaaaataa gctagtgctt ctgtcacaat atgactacca
    25621 atccctttcc tctgataggc ttgatcaacc ataaacaaac caataaaaac agtctcctca
    25681 tcaggatgtg catagacaaa atccataaca gccacaaggt cagatccatt ccaaaatcca
    25741 ataaaaaact tatcagcctt agctttacct tcaggtagac aaagcatgtc ctcttttaca
    25801 gttgcaaaat ttggctctgg tggacaatgc tgaaaataca gaggattact ttcatataaa
    25861 gataaaatac ttggaatatt cttttcagtt agtatcctac aactgtaata cttagatagt
    25921 tggtcaatca tcttttcaaa ttcctacttt cttgtgccct gtgattatga cacaggaaga
    25981 tgcactgatc gtcatcagcc acataaaagt tctttccatc gtgcctaatc gttgtctcaa
    26041 acctttggat aaaaccttta gcctatacaa ctggattttc ctctctcaac agtatattct
    26101 tttgcaggcg aacttcctca aaatcagtcg tgtgcaactt cagtagaata ttcataggct
    26161 cggataatct gagcgacaac tggatggcga accacatcct tggctgaaaa atgaacaaag
    26221 tcaatctgat ggatgttctt gagtttctct tgagcatcaa tcaaaccgga cttgacatta
    26281 cgtggcaggt caatctgact gatatctcca ttgacaatca tcttagaatg aaagcctaaa
    26341 cgcgtcaaga acatcttcat ctgcatgatg gtcgtgtttt gcgcctcatc gagaatgaca
    26401 aaggcatcat ccaaggtccg gccacgcata taggcaaggg gcgcaatttc gataatttca
    26461 cgctccatga gacgagtcgt ttggtctttc ccaagaattt gatacaaggc atcgtaaaca
    26521 ggacgaaggt aaggatccac cttctcctta agatcacccg gaagaaatcc aagactctct
    26581 cccgcttcca ccgctggacg agttaggata attcgcttga cttgcccacg tttaagggca
    26641 gtcactgcca aaataactgc aagaaaggtc ttccccgtcc ctgctggccc aattccaaag
    26701 gtcacatcat gctgtttgac actgtccaca taaagctttt gccctagggt tttgactcgg
    26761 ataggcttcc ctgtattgtc cttgatgatt tcttcttcgt aaagggcgac aaacttgtcg
    26821 atttcatcat ttttgaccat actaatagcc gtcactacat ctggcgtacc aacggtcatc
    26881 ccacgattta ccaaggccat gagagcttga ataacctgac gggcttcctc gcaagcagac
    26941 tcttctccca aaacctggac aatctccgta cgagcatgaa tcacaacatc aagctcttct
    27001 tccatcaaac gaagatggcg ttcattggaa ccaaaaagat gaaacaggtc atctggatga
    27061 ctcagttgaa tgtctattga atgttccttc aaataaagaa cctctctaat ccttttattt
    27121 ctttttatta tagcagaggg atggagaaat tactagtcca aacccaatga ataaatgcta
    27181 gtgttctaga tattcctgcc aaatgctgtc aaaatctcct aggttaaaag agaaactggc
    27241 atgctcctcc aaaaagcgac tgacctcatc aaaatcatct gtgtgttttg gaaaagctgc
    27301 ctcttcaaaa gctaggtctg ccaagatagc tttgggactg ttacttttag gattgcgctc
    27361 ggtcatgagc caagtgtaaa atgattttct cataaatctc cctaaatcaa ctctgtccca
    27421 aactggtctt gcttgatttt accagccttc ataagaccac ctaaagcttt cttgaactga
    27481 cctttagaaa tgccaaaggt tgccttgatg tcgtctggag atgacttatc atttaaggtc
    27541 atgaaaccgc cattgctttc caaataagtc aaaatcatct gagcatcgtt ttccaacatt
    27601 tcaaaggagc gtggtttgag ggagaggttc agagtgcggt ccacttcacg gaaaccaata
    27661 acgcgcgcat ctaatacttg ccccaaacgt ggctctgcgt aacgctcgct aggatgaata
    27721 aaaccaagca tattattttc tggtaggtaa acaaaagttc ctgacagctt gagacggtaa
    27781 acaatggctg gccagttttg gttctgcatg ttgttgtaag caggacgagc gagacgttgg
    27841 aagtcttctt gataagccaa gaggccccag atacggtctt tcttatccac ttcaagacgg
    27901 atgtagagtt ggtcgccctt cttaggccag agttccttga gctcagggag aatatcgagt
    27961 gacacaacga tttccttgtc aggaaggcct gtatccacaa agacacccaa gtccttacga
    28021 acctctgtga cacgtcccca accaaattgg tcctgagtgg cagtcacttc taaggttgtc
    28081 aggcggagtt tttgcttcat atccgtgtat gcaaaacctt tgaccgtatc ccctactgta
    28141 tgttggccct cttccttagc aagagcatag gtttgaccat ccttttgcac aaagtaaaaa
    28201 cggtcatttt catcgatgat cagtccaacg ataaaacttg caagatttgt attcatattt
    28261 ccttctttcg aataaaactc agccagcaat gccaactgag tttttctgtt tatttttaga
    28321 cttccaaaag ttctttctct ttgttggcag tcatatcgtc gatgtgttta acagcatcgt
    28381 ctgttacttt ttgaatgtct ttttcaagag tcttcaattc gtcttcagtg atttcttttg
    28441 ctttttcttg tttcttagct tcgtccatag catcgcgacg gatattgcgg acagccactt
    28501 tagcattttc gccgaccttc ttcacttctt tagcaaggtc acgacgagtt tcttctgtaa
    28561 gaactgggat aatcaagcga atcacagaac cgtcattagc tggtgtgata ccaagatcag
    28621 aagcgttcaa ggcacgttcg atgtctttca atgaagactt gtcaaatggt gttaccaaca
    28681 aaacacgcgc ttctggaatc gtaattgaag cgatttggtt aagaggagtt tcgactccat
    28741 agtattctac atgtacacgg tcgagcaagc ttgcattggc acggccagca cggataccac
    28801 caaattcacg agcaagtgat tggtgagact gggtcattct ctctttagct ttttcaataa
    28861 ttacgttagc cataatcttt cttcttcctt ttcttagata ttatttgaaa ctgttgttcc
    28921 gatattttca ccaaatacga cacgtttgat gttgcctggt tggttcatgt tgaagacaac
    28981 caagtcaatg tcgttatcca ttgagagggt tgaagctgtt gagtccatga tacgaagacc
    29041 tttgttgata acatcacggt gggtcaattc ttcaaactta acagctgtct tatctttctt
    29101 aggatcggca ttgtaaacac catcgacacc atttttagcc ataagaatgg catccgcttc
    29161 gatttcagct gaacgaagag ccgctgttgt atcggttgag aagtaaggtg aaccaattcc
    29221 agcaccaaag ataacgatac ggcctttttc aaggtggcga agagcacgtc cacgtacgta
    29281 aggttctgcc acttgttgca tagcaatggc tgtttgcaca cgcgtatcga caccaacttg
    29341 ttgcaatgaa tctgccatca caagagcatt cataacagtc ccaagcattc cagtgtaatc
    29401 tgcctgaaca cggtccatac ctgcttctgc tgcaggttct ccacgccaga gatttcctcc
    29461 accaataaca agggcaattt cgatacctaa gctatgaact tcttgaatct cttttgcgat
    29521 tgtttgaact gtttggatat caatccctac gccacgttca ccggcaaggg cttcacctga
    29581 taacttgatt aaaatacgtt tatacttggg attcgccatt ttcactctcc ttctttcatc
    29641 ctacctattt tatcacaatt tctaagattt ttatagtatc atgagcaatt ctttcaaaaa
    29701 aattagacag tcaaaaattc ctctaagtcg gcaagggcac gctctgcaat tttttcataa
    29761 cgagccttct tatcacggat acgctcgcct tccaactcct tgatgatccc aaaattgaca
    29821 ttcattggtt ggaaatgttt gctgtcggca tgggtaatgt aatgagctaa gcttccaatc
    29881 gctgtcgtct cggggaaaat aacctcgctt tcttccttga agagacgagc tgcgttaatt
    29941 cccgcaacta agcccgaagt cgccgactca acatagcctt ccacccccgt catctgacca
    30001 gcaaagaaga gatttggttg tttcttggaa cggtaagtct gctcaagaag atttggtgaa
    30061 tccatgtaag aattgcgatg catgacacca taacggacaa actccgcatt ttcaagaccc
    30121 ggaatcattt ggaagacacg cttttgttct ccccatttga ggtgggtctg gaaaccaaca
    30181 atattgtata ggctaccagc tgcattatcc tgacgaagtt gcacaaccgc ataaggtgtt
    30241 ttaaattctc catcacgagg tcctgtgtag tcgtccggat actcaagacc gactggcttc
    30301 atagggccat aaagcatagt tttaatgcca cgtttggcca taacttcgat tggcatacat
    30361 ccttcaaagt acttttcttt ttcaaaagaa ttgagcggtg cttcttccgc attgaccaag
    30421 gcttcatgga aatccataaa ctcttgcttg gtcattggag cattgagata ggctgcttct
    30481 cccttatcat aacgagattt gagatagacc ttactcatat cgatagtgtt gacatcgata
    30541 ataggcgctg ctgcatcgta gaaatagaag ccatcgccat tattaagggc atgaatcttt
    30601 tcagccaggg catcgctagt caagggacca gtcgccacaa ctgtaataac atctgttggc
    30661 aattctgtaa tttcatcacg caccacttca atcaaggggt ggttgaccac tttttcggtc
    30721 accatttgag agaaaccatc acgatccacc gcaagggctc caccggcagg aacacgtgta
    30781 gcctcagcag attccaagat aacagaaccc aagcgacgca tttcttcctt gagaagacca
    30841 actgcatttg tcaaagcatc cccacgcaaa gaattggaac aaaccaactc agcaaaattg
    30901 tctgttttgt gctgaggagt cgacttgaca ccacgcattt cataaagttt aactggaata
    30961 ccacgctctg cgatttggta agctgcttca gaacctgcca aaccagcacc gataacattg
    31021 atataagatt gagacacgac actaatacct ctttgggagt gtgaagttaa gattcacatt
    31081 gaaaaagcca atcagactta cgagctttcg agtttcttgg ctcaggctga aaaagtccat
    31141 tggacttact tcgctctcga atttcttggc tcaggctgaa aaagtccccc agactttcct
    31201 cgctctcgaa tttcttggct caagctgaaa aagtcccccg gactttttca ctcccacaaa
    31261 tctttctatt tttttcttct actagtataa caaaaaaagg gaagaaggca aacttccctg
    31321 tttagtcatt ttcttgatgt aaagagatag agagtattcc agttaagaat caattatatg
    31381 ctactctata aaatcttttc cacataacgg atcgataggg actgttattc tatctcttgc
    31441 tataaccgta ttatctaaaa cagcataaca ttcaacatag tggtctcctt taaactgaga
    31501 atcttcgtga tgttttattt tacctgaaaa aatagcacca cgctcacaat ttcttctaat
    31561 cgcctcagct ccaatatttc tcactttcca ataatattta acatcagaca aaagattatc
    31621 tggaatatct gattttataa tttcaaaatc aagagatttt ctatttttaa tcttaaatct
    31681 actttttaaa aagtctgaaa gaagtgcttt tcgaaaacca tcttgtgtaa tttcacactc
    31741 caaaacaata ttatattcga tattaacatt aaacaattca tcaataaatt cttcattcga
    31801 agctcgattt gttgtagaag tagattgagc gaattttcta ccaaatagct gtctataaac
    31861 ttcagtaagt ttctcttctg aagcgtcttc tattttcttt agagcctgct tagctttatt
    31921 gacaaatttt gctccatcat tatta
//
LOCUS       DAVKDZ010000019        29531 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000019 DAVKDZ010000000
VERSION     DAVKDZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29531
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.019"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(1..593)
                     /locus_tag="VWZ30_000848"
                     /pseudo
     CDS             complement(1..593)
                     /locus_tag="VWZ30_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436461.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            787..1284
                     /locus_tag="VWZ30_000849"
     CDS             787..1284
                     /locus_tag="VWZ30_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU9334621.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
     gene            1284..2939
                     /gene="dnaX"
                     /locus_tag="VWZ30_000850"
     CDS             1284..2939
                     /gene="dnaX"
                     /locus_tag="VWZ30_000850"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU9334622.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2967..3161
                     /locus_tag="VWZ30_000851"
     CDS             2967..3161
                     /locus_tag="VWZ30_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEU9334623.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            3311..4081
                     /gene="sufC"
                     /locus_tag="VWZ30_000852"
     CDS             3311..4081
                     /gene="sufC"
                     /locus_tag="VWZ30_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU9334624.1"
                     /translation="MSVIEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDMVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            4109..5371
                     /gene="sufD"
                     /locus_tag="VWZ30_000853"
     CDS             4109..5371
                     /gene="sufD"
                     /locus_tag="VWZ30_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU9334625.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIESLELPVIERVKFH
                     RWNLGDGTITESEPSANVPDFTALDNHLKLVQVGTQTVFEQIPVELAQQGVVFTDFHS
                     ALEEIPELIEELFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEIKEPIEGVFYQDS
                     DSDVPFNKHIMIIAGKNSKISYLERLESRGEGSTKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQIQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            5382..6608
                     /locus_tag="VWZ30_000854"
     CDS             5382..6608
                     /locus_tag="VWZ30_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9334626.1"
                     /translation="MLDVEVIRKDFPILDQIVNDEPLVYLDNAATTQKPLAVLETINR
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     YAEEVLTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMVDLRAKLTD
                     KVQFVSLAHASNVLGVVNPIKEITQMAHQVGAIMVVDGAQSTPHMKIDVQDLDVDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MVGAIGLAAAVDYLEKIGMDAIEAHEQELIAYVFPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLDVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            6595..7035
                     /locus_tag="VWZ30_000855"
     CDS             6595..7035
                     /locus_tag="VWZ30_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU9334627.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFNAEDRLEDIAFLNSGCTISTASASMMTEAVLGKTKQEILELATIFSEMVQGQ
                     KDDRQDSLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            7093..8505
                     /gene="sufB"
                     /locus_tag="VWZ30_000856"
     CDS             7093..8505
                     /gene="sufB"
                     /locus_tag="VWZ30_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU9334628.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEDVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDEVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFEKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANVGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSKVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8736..9977)
                     /locus_tag="VWZ30_000857"
     CDS             complement(8736..9977)
                     /locus_tag="VWZ30_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU9334629.1"
                     /translation="MKKIFLTLLTISLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLRDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLESMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVAHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            10080..11189
                     /locus_tag="VWZ30_000858"
     CDS             10080..11189
                     /locus_tag="VWZ30_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9334630.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVRVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            11835..12182
                     /gene="ssrA"
                     /locus_tag="VWZ30_000859"
     tmRNA           11835..12182
                     /gene="ssrA"
                     /locus_tag="VWZ30_000859"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(12352..12439)
                     /locus_tag="VWZ30_000860"
     tRNA            complement(12352..12439)
                     /locus_tag="VWZ30_000860"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12403..12405),aa:Ser,seq:gga)
     gene            12601..13341
                     /locus_tag="VWZ30_000861"
     CDS             12601..13341
                     /locus_tag="VWZ30_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9334631.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQGIKEKTEVIEV"
     gene            13338..14249
                     /gene="pfkB"
                     /locus_tag="VWZ30_000862"
     CDS             13338..14249
                     /gene="pfkB"
                     /locus_tag="VWZ30_000862"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEU9334632.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            14246..16198
                     /locus_tag="VWZ30_000863"
     CDS             14246..16198
                     /locus_tag="VWZ30_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9334633.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            16362..18587
                     /locus_tag="VWZ30_000864"
     CDS             16362..18587
                     /locus_tag="VWZ30_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU9334634.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(18631..19059)
                     /locus_tag="VWZ30_000865"
     CDS             complement(18631..19059)
                     /locus_tag="VWZ30_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334635.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITATITIMN
                     ALDNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVKE
                     EKKEEQKTATQDSTTPATSKPATENEKQPNTPTSENKSNQ"
     gene            19197..20339
                     /locus_tag="VWZ30_000866"
     CDS             19197..20339
                     /locus_tag="VWZ30_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9334636.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHLAVK
                     ESALWLKSQGFEVDFAPVDKKGFVDVEALADLIRPDTTLVSIVAVNNEIGSVQPIEAI
                     SELLADQPTISFHVDAVQALAKIPTEKYLTERVDCATFSGHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFIFSAEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            20348..21562
                     /gene="thiI"
                     /locus_tag="VWZ30_000867"
     CDS             20348..21562
                     /gene="thiI"
                     /locus_tag="VWZ30_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU9334637.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVR
                     ADRDRAHAHLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            21698..22522
                     /locus_tag="VWZ30_000868"
     CDS             21698..22522
                     /locus_tag="VWZ30_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9334638.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFYSKESFIGHSWKIIPAIKRNSDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     KSPIPGQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYIECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            22544..23284
                     /locus_tag="VWZ30_000869"
     CDS             22544..23284
                     /locus_tag="VWZ30_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9334639.1"
                     /translation="MHIEYLSHWSGHLNREMYLNRYGHAGIPVVVFASSGGSHNEYYD
                     FGMIDACTSFIEEGRVQFFTLSSVDGESWLATWKNAHDQAEMHRSYERYVIEEAIPFI
                     KHKTGWFDGMMTTGCSMGAYHALNFFLQHPDVFTKVIALSGVYDARFFVGDYYNDDAI
                     YQNSPVDYIWNQNDGWFIDRYRQAEIVLCTGLGAWEQDGLPSFYKLKEAFDKKQIPAW
                     FAEWGHDVAHDWEWWRKQMPYFLGNLYL"
     gene            23297..24463
                     /locus_tag="VWZ30_000870"
                     /pseudo
     CDS             23297..24463
                     /locus_tag="VWZ30_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
     gene            24971..25936
                     /locus_tag="VWZ30_000871"
     CDS             24971..25936
                     /locus_tag="VWZ30_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9334640.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDTV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLTNPPFKGSLDYNSTYNDLLATVKTKKQNYSFFLFSCEL"
     gene            25924..26463
                     /locus_tag="VWZ30_000872"
                     /pseudo
     CDS             25924..26463
                     /locus_tag="VWZ30_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            26476..27061
                     /locus_tag="VWZ30_000873"
                     /pseudo
     CDS             26476..27061
                     /locus_tag="VWZ30_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            27121..27357
                     /locus_tag="VWZ30_000874"
     CDS             27121..27357
                     /locus_tag="VWZ30_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEU9334641.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            27354..27767
                     /locus_tag="VWZ30_000875"
     CDS             27354..27767
                     /locus_tag="VWZ30_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEU9334642.1"
                     /translation="MTIYLTEKQIEKINSLAIQRYSPNEKIQTVSPSALNMIVNLPEQ
                     FVFGKPLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            27885..28839
                     /locus_tag="VWZ30_000876"
                     /pseudo
     CDS             27885..28839
                     /locus_tag="VWZ30_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            28873..29508
                     /locus_tag="VWZ30_000877"
                     /pseudo
     CDS             28873..29508
                     /locus_tag="VWZ30_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         8929 a         5303 c         6447 g         8852 t
ORIGIN      
        1 tcgaatgata gcttgtgttc ttccctcaag aacagtgatg atattgagat tgttaaaatc
       61 ttgcgcaatg aagctcatct ccatctcccg attgaaacag tcactccccg gactgtttca
      121 acctcccaag acataatctc aggcagattt ctaaaattac actcaaagtg gaagtcattg
      181 agcttgcgaa tgacagttga agttgaaatg gccagctgat gagcaatatc agtcatagaa
      241 attttttcaa ttaacttttg agcaattttt tggttgatga tacgagggat ttggtgattt
      301 ttctttacca ggggagtctc agcaaccatc atttttgaac agtgatagca cttgaaacgg
      361 cgttttctaa ggagaattct agaaggcata ccagttgttt caagataagg aattttagaa
      421 ggtctttgaa agtcatattt cttcatttga cttccgcact cagggcaaga tggggcgtcg
      481 tagtccagtt tggcgatgat ttctttgtgt gtatctttat tgatgatatc tagaatcttg
      541 atgtttgggt ctttaatatc gagcagtttt gtgataaaat gtaattgttc catatgattc
      601 tttctaatga gttgttttgt cgcttttcat tataggtcat atgggacttt ttttctacac
      661 aaaaataggc tccataatat ccatagggaa tttacccact acaaatatta tagagcccaa
      721 aatcctttgt ttactaaaca agggattttt cttttgtctc tgctcctttt ttgatataat
      781 agttctatgt taaaatcaga aaaacaatca cgttatcaaa tgttaaatga agaattgtcc
      841 ttcctattgg aaggcgaaac caatgttttg gctaatcttt ccaacgccag tgctctcata
      901 aaatcacgtt ttcctaatac cgtatttgca ggcttttatt tgttcgatgg aaaggaattg
      961 gttttaggtc ccttccaagg aggtgtttcc tgcatccgta ttgcactagg caagggtgtt
     1021 tgtggtgagg cagctcactt tcaggaaact gttattgttg gagatgtgac gacctatctc
     1081 aactatattt cttgtgatag tctagctaaa agtgaaattg tggtgccgat gatgaagaat
     1141 ggtcagttac ttggagttct ggatctggat tcttcagaga ttgaggatta cgatgctatg
     1201 gatcgagatt atttggaaca atttgtcgct attttgcttg aaaagacagc atgggacttt
     1261 acgatgtttg aggaaaaatc ttaatgtatc aagcacttta tcgaaaatat agaagtcaaa
     1321 acttctccca gttagttggt caagaagttg tggctaagac tcttaaacaa gcggtggagc
     1381 aagagaaaat aagtcacgct tatctttttt ctggtcctcg tggaacggga aaaaccagtg
     1441 ttgctaaaat ctttgccaag gctatgaact gtcccaatca agtgggtggc gaaccttgca
     1501 ataactgcta tatttgtcaa gcagtgacgg acggtagttt agaagatgtc attgaaatgg
     1561 atgcagcttc taataatggg gtagatgaaa ttcgcgaaat tcgtgataaa tctacctatg
     1621 cgcctagcct tgctcgttat aaggtttata tcatagatga ggttcacatg ctgtctacag
     1681 gggcttttaa tgccctccta aagacgctgg aagaaccaac acagaatgta gtctttattt
     1741 tggccactac tgaattgcac aagattcctg ctactattct atcccgtgtg caacgttttg
     1801 agtttaaatc aattaagaca caggatatta aggaacatat tcactatatc ttagaaaaag
     1861 aaaatatcag ttctgaacca gaggctgtgg aaatcattgc cagacgggcg gaaggtggaa
     1921 tgcgggacgc cttgtctatt ttggatcaag ccctgagttt gacacaggga aatgagctga
     1981 cgactgctat ctctgaagaa attactggca ccattagcct atcagccttg gatgattatg
     2041 tggcggcctt gtctcaacag gatgttccca aagctttgtc ttgcttgaat cttctttttg
     2101 acaatggtaa gagcatgact cgttttgtga ccgatctttt gcactattta agagacttgt
     2161 taattgttca aacaggggga gaaaatactc atcatagttc agtctttgta gaaaatttgg
     2221 cacttcctca aaaaaatctg tttgaaatga ttcgcttagc aacagtgagt ttagcagata
     2281 ttaagtctag tttgcagccc aagatttatg ctgaaatgat gaccgtccgt ttggcggaaa
     2341 tcaagccaga accagctcta tcaggagcgg ttgaaaatga aattgctacg ctgagacagg
     2401 aagttgcccg tctcaaacaa gagctttcta atgtaggtgc ggttcctaaa caagttgcac
     2461 cagctcctag tcgaccagct acgggcaaaa cagtctatcg tgtcgatcgt aataaagtgc
     2521 aatctatctt acaagaggcc gtcgaaaatc ctgatttagc acgtcaaaat ctaattcgtt
     2581 tgcagaatgc ctggggagag gtaattgaaa gtctaggtgg gccggacaag gctctgctag
     2641 ttggttctca accggttgct gccaatgaac accatgctat tcttgctttt gagtctaact
     2701 tcaatgctgg tcaaactatg aaacgagaca atctcaatac catgtttggt aatatcctca
     2761 gtcaggcggc aggtttttca cctgagattt tagctatttc catggaggaa tggaaagaag
     2821 ttcgcgcagc cttttcagcc aaagccaaat cttctcaaac tgaaaaagaa gtagaagaaa
     2881 gcctgattcc agaaggattt gaatttttgg ctgataaagt gaaggtagag gaagactaaa
     2941 gaaagatttc atgatacaat aagtttatga ataaacaaca atttattatt atggcgctgt
     3001 ttacagctgc tgagacctat tttttcaatg aagcctggat gactggccgc tatattatgg
     3061 cagccttttg ggcaatttta ctctttagaa atttccgagt cagttatgtg atgggtaaaa
     3121 tcgttgatgt catcgatcag cattttaata ggaaagacta gccctcagct tccagacaaa
     3181 atcaaagcct tttaggcttt tttttgttat actagaaaag tatatttata gaatttttgc
     3241 tctatttctg gggaaatcag acgtttttct agtaagtact gtaaaagttt tgaaaaagaa
     3301 aggaactatt atgtcagtaa tagagatcaa agatcttcac gttgagattg aaggaaaaga
     3361 aattttaaaa ggggttaacc tgaccctgaa aacaggagaa attgccgcta tcatgggacc
     3421 aaatggtaca ggtaaatcga ctctttctgc cgctatcatg ggaaatccaa actatgaagt
     3481 aactaaaggt gaagttttgt ttgatggcgt aaacatcctt gagttggaag tggatgagcg
     3541 tgcgcgtatg ggacttttcc ttgctatgca atacccatca gaaatccctg gaattaccaa
     3601 tgctgagttt cttcgtgccg ctatgaatgc gggtaaagaa gatgatgaga agatttcagt
     3661 tcgtgagttt attactaagc tagatgaaaa aatggaattg ctcaacatga aagaagaaat
     3721 ggcagagcgt tacctcaacg aaggcttctc tggtggtgag aaaaaacgca atgaaattct
     3781 tcaacttttg atgctggagc caacatttgc tcttttggac gagattgact caggtcttga
     3841 tattgacgct cttaaagttg tgtctaaagg tgtcaatgcc atgcgtggtg aaggttttgg
     3901 tgctatgatc attactcact accaacgtct tttgaactat atcacacctg atatggtaca
     3961 cgtgatgatg gaaggtcgtg ttgtcctttc tggtggtcca gaattggctg cgcgtttgga
     4021 acgtgaagga tacgcaaaat tagctgaaga acttggctac gactacaagg aagaattgta
     4081 attccctcgt atcttttagg agaagtaaat gactagagaa aatattaaac ttttttcaga
     4141 aatgcacgct gaaccaagct ggttggctga tctccgtcaa aaagcttttg ataaaatcga
     4201 gagtttggaa ttaccagtta ttgagcgtgt gaaatttcac cgttggaatc tgggtgatgg
     4261 aacgattaca gaaagtgagc catcagcaaa tgttccagat ttcactgcac tagataatca
     4321 cttgaagttg gtgcaagtag gaactcaaac tgtttttgag caaattccag ttgagttggc
     4381 tcaacagggt gttgtattta cagacttcca ttcagcttta gaagaaattc cagagcttat
     4441 tgaggaatta ttcatgtcat ctgtcaagta tgacgatgat aagttggcag cctaccatac
     4501 agcttatttc aacagtggtg ctgttcttta cattcctgat aatgttgaga ttaaagaacc
     4561 aatcgaaggt gttttctacc aagacagtga tagcgatgta ccgtttaaca agcatattat
     4621 gattatcgct ggtaaaaatt ctaagattag ttatctggag cgtttagagt cacgtggtga
     4681 aggaagtaca aaagcaactg caaacatcac tgtcgaagtg attgcacgtt ctggtgctca
     4741 agtgaagttt gctgccatcg accgtctagg tgaaaacgtt actgcctaca ttagccgtcg
     4801 tggtaaatta ggcaatgatg caagcattga ctgggcaatc ggtgtcatga acgaagggaa
     4861 cgtcgttgcg gactttgata gcgacttgat tggaaatggt agccatgctg acctgaaagt
     4921 tgtagctctt tcaagtggcc gtcagataca agggattgat acccgagtga ctaactatgg
     4981 ttgcaactca atcggaaata tccttcaaca tggggttatt cttgaaaaag caaccttgac
     5041 cttcaatggt attggccaca ttatcaaggg tgccaaggga gcagatgcgc aacaagaaag
     5101 tcgtgttctc atgctttcag accaagcgcg ttcagatgcc aatccaattc ttttgattga
     5161 tgaaaatgac gtaactgcag gacatgcggc ttctatcggt caggtagatc cagaagacat
     5221 gtactacctc atgagtcgtg gtttggacaa ggcaactgca gagcgtttgg ttgttcgtgg
     5281 tttccttgga tccgttatcg ttgagattcc agtcaaggag gttcgtgatg aaatgattgc
     5341 gactatcgaa gaaaaattgt caaaacgcta aggggtagcc tatgttagat gtagaagtga
     5401 ttcgcaagga ttttccaatt ttagaccaga ttgttaatga tgaaccattg gtttatttgg
     5461 acaatgctgc gacgacacaa aaaccactag cagttctgga aacaatcaac cgctactatg
     5521 agcaggacaa tgccaatgtt catcgtggcg ttcatacctt ggcagaaagg gcgacagctt
     5581 cttatgaggc agctcgtgaa accattcgta agtttatcaa tgcagggtct acaaaggaag
     5641 ttctctttac tagaggaacg acaaccagtc ttaactgggt agcgcgttat gctgaggaag
     5701 tcttgactga gggagaccag gttttgattt ctgtcatgga acaccattct aatatcattc
     5761 cttggcagga agcctgccgt aagactggag cggagcttgt ctatgtttat ctcaaagacg
     5821 gagctttgga tatggttgac ttgcgagcaa aattgacgga caaggtacag tttgtttccc
     5881 tagctcatgc ctcaaatgtt ctgggtgtgg tcaatccgat caaagaaatc actcaaatgg
     5941 cccaccaagt gggagccatc atggtggtgg atggtgctca gtctactcct catatgaaga
     6001 ttgatgttca ggacttggat gtggacttct ttgccttttc aggtcacaag atggctggtc
     6061 caactggtat cggtgttctt tacggcaaag aaaagtatct ggaacaaatg tcaccagttg
     6121 aatttggcgg cgaaatgatt gatttcgttt atgaacaatc tgctagttgg aaggaattgc
     6181 cttggaaatt cgaggctgga actcctaaca tggttggtgc aatcggactt gctgctgcag
     6241 ttgattatct ggaaaagatt ggtatggatg ccattgaagc ccatgaacag gaattgatcg
     6301 cctatgtctt tccgaaatta caggcgattg aaggactgac catttatggt tcacaggact
     6361 tggctcaacg ttcaggtgtc attgctttta acctaggcga ccttcatcct cacgaccttg
     6421 caacggcttt ggattatgaa ggtgtggcag ttcgtgcggg tcaccactgt gcgcaaccct
     6481 tgctccagta tttggatgtt ccagcaacag ctcgtgcaag tttttatatc tacaatacca
     6541 aggcagattg cgacaagcta gtcgatgcct tacaaaagac aaaggagttt ttcaatggca
     6601 ctttctaaac tagatagcct ttatatggca gtggtagcag accattcgaa aaatccacac
     6661 caccaaggga agttggaaga tgctgagcaa atcagtctca acaatccgac ctgtggcgat
     6721 gttatcaacc tctctgtcaa gtttaatgca gaagaccgtt tggaagatat cgcttttcta
     6781 aactcaggtt gtaccatctc aactgcctct gcaagcatga tgacagaggc agttttaggc
     6841 aagaccaagc aagaaattct agaacttgca accatctttt ctgaaatggt tcaaggacaa
     6901 aaagatgaca gacaagatag tcttggagat gcagccttct tgtcaggtgt tgctaaattt
     6961 cctcaacgaa tcaagtgtgc gaccctagct tggaacgccc tgaagaaaac aattgaaaat
     7021 caagaaaaac agtaaggcaa gtttcttttg tcttatgaat cagtagaaat gaagaatgaa
     7081 agaaaggata ttatggctga agaaagagta gaaccaaaac caattgatct tggtgaatat
     7141 aaatttggtt tccatgatga tgtagagcct gtcctatcga caggaaaagg attgaatgaa
     7201 gatgtcattc gtgaactatc agctgccaag ggagaacctg agtggatgtt agagttccgt
     7261 ttgaagtctt atgaaacctt taaaaaaatg cccatgcaaa cttggggagc agacttgtca
     7321 gagattgact ttgatgactt gatctactac caaaaaccat ctgataaacc agctcgttca
     7381 tgggatgagg ttcctgaaaa aattaaagaa acctttgagc gtatcgggat tcctgaagct
     7441 gagcgtgctt atctagccgg tgcctctgcc cagtacgagt cagaagtggt ttaccacaac
     7501 atgaaggaag aatttgagaa gttaggtatt atctttacag atacggattc tgccctcaag
     7561 gaatacccag acttgtttaa acaatacttt gctaagttgg taccgccaac agataacaaa
     7621 ttggctgccc ttaactcagc agtatggtct ggtggaacct ttatctacgt accaaaaggg
     7681 gtcaaggtag atattccact tcaaacttac ttccgtatca acaacgagaa tacaggtcag
     7741 tttgaacgta ccttgattat tgttgatgag ggagcaagtg tccactacgt tgaagggtgt
     7801 acagcgccaa catattcaag caacagcttg cacgctgcca tcgtagaaat ctttgccttg
     7861 gatggtgctt atatgcgtta tacaaccatc caaaactggt ctgataacgt ttataacttg
     7921 gtaacaaagc gtgctaaagc tcaaaaggat gccactgttg agtggattga tggtaacttg
     7981 ggtgccaaaa caaccatgaa atacccatct gtttaccttg atggagaagg ggcgcgtggg
     8041 actatgcttt cgattgcctt tgctaatgta gggcaacacc aagatacggg tgctaagatg
     8101 atccacaatg ctccacatac tagctcatct atcgtgtcta aatccatcgc taaaggtgga
     8161 ggtaaggttg attaccgtgg tcaagtaacc tttaacaaga actctaagaa atctgtttct
     8221 cacattgagt gtgataccat tatcatggat gacttgtcag catcagatac cattccgttt
     8281 aatgaaatcc acaactcgaa agttgctttg gaacacgaag caaaggtttc taagatttca
     8341 gaagagcaac tctattacct catgagccgt ggattgtcag aatctgaggc aactgagatg
     8401 attgttatgg gatttgtaga gccctttacg aaagaacttc caatggaata cgcagttgag
     8461 ctgaaccgct tgattagcta tgaaatggaa gggtcagttg ggtaaaattt gattttatac
     8521 tcttcgaaaa tctcttcaaa ccacgtcagc atcgccttac cgtatgtatg gttactgact
     8581 tcgtcagttt catctacaac ctcaaaacag tgttttgagc aacctgcggc tagcttccta
     8641 gtttgttctt tgattttgag tattagattt actcaaaatc aaggattttg aagatgaact
     8701 tgtatcaaaa aatcgcggtt taaaatcgcg attttttata atttctcgtt aacaaagcgg
     8761 acaaactgat tccaccaaac ttttaagaaa aaggcttttt caattttctt gtctgctacc
     8821 atttcgaaac tagggcgctc tgtggtgatg taaccttgac caatcaagtc cttgtcttca
     8881 taagtcaaat gggcaaccac tgttccagct tcaagtggtg ctgggattgc tttggaatca
     8941 ggtgtgaatt gaacagattg ggaagattga ttcccaacac gttcgattag atagatatcc
     9001 tctggagcca ctgcagttac tgtatcttct tttccatctt gtacaggggc tttgctatct
     9061 tgataggcat cgccttgttg aacgattttg cgaagtgtaa atgtagaaga aatataatcc
     9121 attagggaag atgtagctgt aaatcgagcg taaggattat tgtcttgatg atctgcattt
     9181 aaaacaactg tgatgactct catgcctttt tcgacagtag taccaacaaa agactctcca
     9241 gccttatctg ttgttcctgt ttttagccca tcaaaaccac cacggtaagc aggcatactt
     9301 tctaacatgt agttggttga agtgattgtc attccagcaa aagtagaaga aggttttttg
     9361 gtgatttcca agacttgtgg gtattttttg atgaggttgc gagcaacaat agcgacatca
     9421 taagcactaa gcttattttc ctcatctttt ttagaacctg ggtaaatgtt atcccttaga
     9481 gtttcattgt taagacctgt cgtattgaca acagtggcat cctgaattcc ccattccaag
     9541 agttttgccc gcatcatatc gacgaaatct ttttctgagc cagcaatttt ctcagctagg
     9601 gcaatagcgg cgctgttggc actagatacc agagttgctt caagcaactc ttcgacagta
     9661 taattacggg cctccatagg aatattactg gcttcagaat ttgtcgtcaa ttgataagga
     9721 taatcagaaa tatctacagg agtggagagg gtaatacttc cgttttccaa agcttcatag
     9781 accagataaa cagtaatcaa ttttgttatg gaagcaattt cgacaggttg cgttgcatcc
     9841 ttctcataga gaattttacc agtatttgcc tcaacagcaa tcgcatgttt agcggcaatg
     9901 gtaaaatctt gagcaacagc agtagaagca ccccctaaaa gagagatagt taataaagtt
     9961 aaaaatattt ttttcatagt agtcttattc tatcataaag aaaaaaaata ttcttgcttt
    10021 aataattcat ctgttaagct ttttgaaaat atggtaaaat aaagtgaggg aggtaactca
    10081 tgtttcgtag aaataaatta tttttttgga ccacagaaat tttactctta accatcatct
    10141 tttacctatg gagacagatg gggtctttga ttaacccttt tgttagagtg cttaatacaa
    10201 ttatgattcc atttttatta gggggctttc tttattattt gacaaaccct attgttactt
    10261 tcttaaataa agtctgtaaa ctcaatcgtt tgcttggtat tttaattacc ttgtgtactt
    10321 tggtctgggg aatggtcata ggtgttgtct atctcttacc tattttgatt aatcagttat
    10381 ctagtttgat tatatctagt caaactattt atagtcgagt acaagactta atcatagact
    10441 tatctaatta tcctgcgctc cagaatttgg atgtagaagc tacaattcag cagttaaact
    10501 tatcctatgt tgatattctt caaaatatcc taaatagcgt atcaaatagt gtggggagcg
    10561 tcttgtcagc tcttatcagt actgttttga ttttgattat gactccagtt tttttggttt
    10621 atttcttatt agatggacat aaattcttgc ccatgcttga aagaacaatt ctaaagaggg
    10681 atcgcttgca tattgcaggc ttattaaaga atttaaatgc gacgattgct cgctatatta
    10741 gtggagtttc gattgacgca atcattatag gttgtttggc ttatattggc tatagtatta
    10801 ttggtttaaa atatgcttta gtttttgcca ttttttctgg tgtagccaat ttaattcctt
    10861 atgtgggacc aagtattggt ttgattccta tgatcatcgc aaatatattc actgatcccc
    10921 atagactgct gattgcagtg atttatatgc ttgttgttca gcaggtagat ggcaatatct
    10981 tatatcctcg aatcgtagga agtgttatga aggttcatcc aatcacgatt ttagttttac
    11041 ttttgttgtc aagcaatatc tatggtgtag ttggaatgat tgtcgcagtg ccaacctatt
    11101 ctatcttgaa agaaatttct aagttcttat cccgtttgta tgaaaatcat aaaataatga
    11161 aagaacgaga aagagaatta gctaagtaaa agtcaggaga accctgattt ttctttactg
    11221 gaagtggcct ttagattaga agactgaaaa taagttaaag tcttaaacta attttcacag
    11281 ctaagaatag tagaagttaa tctgataaaa atcgaaaaaa ccagtggaat tccgtgtcag
    11341 ggtaagttcc actggttttc atagtctatt aaagttcgaa tgaaacctat ttatagtata
    11401 ttgaaactag aatagtacac ctctaattct aaaacattgt tagaaatcga tttgactgtc
    11461 ctgatctatt cgtcctattc ttatttcatt ttactatatt ttggtgcaat aagtgaaaag
    11521 tagtccgaat aatataagga ttaattttat agtttttaaa ctcaaatgaa ttaaaataaa
    11581 gagagtacga aaattctcat ctgaaagtat tttagaataa ttctcttcgt gaatttcttc
    11641 aaaacagata gcttcatctt aggtatgtgt taattggatt tcgtaagctc tatttacaat
    11701 cgtaaagtta tgcactgaga aagttgtagt tagtcttcca gtttgctaaa tttctcgaat
    11761 agtatctttt ttaggtatgt gatttctttt tgcatttttg agttagataa ggtataatgg
    11821 ttttattgtc ttttggggtc gttacggatt cgacaggcat tatgaggcat attttgcgac
    11881 tcgtgtggcg acgtaaacgc tcagttaaat ataactgcaa aaaataacac ttcttacgct
    11941 ctagctgcct aaaaaccagc aggcgtgacc cgatttggat tgctcgtgtt caatgacagg
    12001 tcttattatt agcgagatac gattaagcct tgtctagcgg cttgataaga gattgataga
    12061 ctcgcagttt ctaggcttga gttatgtgtc gaggggctgt taaaataata cataacctat
    12121 ggttgtagac aaatatgttg gcaggtgttt ggacgtgggt tcgactccca ccggctccat
    12181 tattcctttg cattcttttg cattccttgg taaaacgttg ttaaatcaac gttttttatt
    12241 tttatctttg gtattccttt gcattctttt gctaaaaagg gagtcacaaa cagaccctat
    12301 tttaaaaaag gatacaacat ttgttgcatc ctaaaaataa tcttttttcg acggaagata
    12361 tgggattcga acccacgcac gctattacac gcctaccgcg tttccaacac ggcctcttaa
    12421 gcctcttgag taatcttcca atacttactc aaatagtcta ccataaaggc tcttatcttg
    12481 caataaaaat tctagaaata agaaaaatga tagattttga aagaaaatga taaaaaatgc
    12541 ttgacttcaa aataaagtat ggtagaatga atagtgtaaa cgataacagg aggtgattca
    12601 gtgttaaaaa cagaacgtaa acaactgatt ttagaggagt taaatcaaca tcatgtagtt
    12661 tctctagaaa aattagttag tttgctagaa acttcagaat caacggttcg aagagacttg
    12721 gatgagttgg aagcggaaaa caagcttcgt cgtgtgcatg gtggagcaga actcccccac
    12781 tccttacagg aagaagaaac cattcaagaa aaatctgtca aaaaccttca agaaaagaag
    12841 ttgctggctc agaaagcagc ctctctcatt aaagaaaaag atgtcatctt tatcgatgct
    12901 ggaacaacaa ctgctttttt gattcatgaa ttggtcaata agaatgttac agttgtgacc
    12961 aactccattc accatgccgc tcagttggtt gaaaagcaga ttccaactgt catggttgga
    13021 ggaaacgtca agacggcgac agatgctagt atcggtggcg ttgctcttaa ccagattaac
    13081 caattgcact ttgaccgtgc ctttatcgga atgaatggtg ttgacgatgg ctattatacg
    13141 actcctgata tggaggaggg agctgtgaaa agagctattt tggagaatgc caagcagacc
    13201 tacgtcttgg tggattcgtc aaaaattgga caaacttgct ttgccaaggt agcaccactc
    13261 aaacgcgcta tcgttatcac tagtcaaggg catgagctct tgcagggtat taaggagaaa
    13321 acggaggtaa tagaagtatg atttatacag tcacactcaa tccatccatt gactatatcg
    13381 ttcgtttgga ccaagtcaaa gttggtagtg taaatcgtat ggacagtgat gataagtttg
    13441 ctggtgggaa aggaatcaat gtcagccgtg tcttgaaacg tttgaatata ccaaatacag
    13501 cgacgggatt tatcggtggc tttactggta aatttatcac agatacttta gcagaggaag
    13561 aaatcgagac acgttttgtc caggtggcag aagatactcg tatcaatgtt aaaatcaaag
    13621 cagaccaaga aacagaaatc aacggaacgg gtccaactgt tgaaccggtt cagctagaag
    13681 aattgaaagc tattttatct agtctgacag cagaagatac agttgtcttt gcaggttcaa
    13741 gtgctaaaaa tctaggcaat gttatctata aggatttgat ttccttgacg cgccagactg
    13801 gtgcgcaagt ggtctgtgac tttgaaggac agaccttaat tgatagtttg gactaccagc
    13861 ctcttcttgt aaaaccaaac aatcatgaac ttggagcgat ttttggggtt aaactcgaaa
    13921 gtttagatga aattgagaaa tacgctcgtg agttactggc taagggtgct caaaatgtta
    13981 ttatctctat ggctggtgat ggcgcccttc ttgtcacatc tgagggagct tacttcgcta
    14041 aaccaatcaa aggaacagtc aaaaattcag ttggagctgg tgattctatg gttgctggat
    14101 tcacaggtga atttgtcaaa tcaaaagacg cagtagaagc cttcaaatgg ggagtggctt
    14161 gcggaacggc aactaccttc tcagatgact tggcaacggc ggaatttatt aaagaaacat
    14221 atggaaaagt tgaggtagaa aaaagatgaa aattcaagac ctattgagaa aagatgtcat
    14281 gttgctagat ttgcaggcaa ctgaaaaaac agctgtcatc gacgagatga ttaaaaattt
    14341 gacagagcac ggttatgtaa cagattttga aacatttaaa gaaggaattt tggcgcgtga
    14401 agctttgact tctactggtt tgggtgatgg aatcgcaatg cctcacagca aaaacgctgc
    14461 tgtcaaagaa gcgacagttc tatttgctaa gtcaaataag ggtgttgact acgagagctt
    14521 ggatggacaa gcaactgacc tcttcttcat gattgcagct ccagaaggtg ccaatgatac
    14581 tcacttggca gccttggcag aattgtctca atacttgatg aaagacggtt ttgcagacaa
    14641 acttcgtcaa gcaacatctg cagaccaagt tatcgaactt tttgaccaag cttcagaaaa
    14701 aactgaggaa cttgttcaag cacctgctaa tgactctggt gactttatcg tagctgttac
    14761 agcttgtaca acaggtattg cccacactta catggcccaa gaagcccttc aaaaagtagc
    14821 tgctgaaatg ggggttggta tcaaggtcga aaccaacggt gctagcggtg ttggaaatca
    14881 actaactgca gaagatatcc gtaaggctaa agctattatc attgcagcag acaaggccgt
    14941 tgaaatggat cgatttgatg gaaaaccatt gatcaatcgt ccagttgctg acggtatccg
    15001 taagacagaa gagttaatta acttggctct ttcaggagat actgaagtct accgtgccgc
    15061 taatggtgca aaagctgcaa cagcctctaa cgaaaaacaa agccttggtg gtgccttcta
    15121 caaacacttg atgagtggtg tatctcaaat gttaccattc gttatcggtg gtggtatcat
    15181 gattgccctt gccttcttga ttgacggtgc tttgggtgtt ccaaatgaaa accttggcaa
    15241 tcttggttct taccatgagt tagcttctat gttcatgaaa attggtggag ctgcctttgg
    15301 tttgatgctg ccagtctttg cgggttatgt tgcctactct attgctgaaa aaccaggttt
    15361 ggtagcaggt ttcgtggctg gtgctattgc caaagaaggt tttgcctttg gtaaaattcc
    15421 ttatgccgca ggtggtgaag caacttcaac tcttgcaggt gtctcatctg gtttcctagg
    15481 tgcccttgtt ggtggattta tcgcaggtgc cttggttctt gccatcaaga aatacgttaa
    15541 agttcctcgt tcactcgaag gtgctaaatc aatccttcta ttgccacttc ttggaacaat
    15601 cttgacagga tttgttatgc tagctgtaaa tatcccaatg gctgcaatca atactgctat
    15661 gaatgacttc ctaggcggtc ttggaggagg ttcagctgtc cttcttggta tcgtccttgg
    15721 tggaatgatg gctgttgaca tgggtggacc agttaataaa gcagcttatg tctttggtac
    15781 aggtacgctt gcagcaactg tttcttcagg tggttctgta gccatggcag cagttatggc
    15841 tggaggaatg gtgccaccac ttgcaatctt tgtcgcaact cttcttttca aagataaatt
    15901 tactaaggaa gaacgtaact ctggtttgac aaacatcatc atgggcttgt catttatcac
    15961 tgagggagcg attccatttg gtgccgctga cccagctcgt gcgattccaa gcttcatcct
    16021 tggttcagca gtagcaggtg gactcgttgg tcttactggt atcaaactca tggcgccaca
    16081 cggaggaatc ttcgttatcg cccttacttc aaatgctctc ctttacctcg tttctgtctt
    16141 ggtaggagca atcgtaagtg gtgtggttta tggttaccta cgcaaaccac aagcataaaa
    16201 aatagaaaaa tgaaaagatt ggaccgtttg gtgcagtctt tttctcttcc cgaaatgcct
    16261 gtgaaatatg gtataataga agaatggcaa acaagaatac aagtacaaca agacggagac
    16321 cgtctaaagc agaactggaa agaaaagaag cgattcaacg aatgttgatt tcgttaggaa
    16381 ttgcgatttt attgattttc gcagccttca aattaggggc tgcaggtata accctttata
    16441 atttaattcg cttgctagtg ggtagcctag cttatctggc gatattcggc ctattaatct
    16501 atctcttctt tttcaagtgg atacgaaaac aggaaggact cttatctggc tttttcacca
    16561 tatttgctgg cttactcttg atttttgagg cctacttggt ttggaaatat ggtttggaca
    16621 agtccgttct aaaagggacc atggctcagg ttgtgacaga tctgactggt tttcgaacga
    16681 ctagctttgc tggagggggc ttgatcgggg tcgctcttta tattccaaca gcctttctct
    16741 tttcaaatat cggaacttac tttattggtt ctatcttgat tttagtgggt tctctcctag
    16801 tcagcccttg gtctgtttac gatattgctg aatttttcag tagaggcttt gccaaatggt
    16861 gggaagggca cgagcgtcga aaagaggaac gctttgtcaa acaagaagaa aaagctcgcc
    16921 aaaaggctga gaaagaggct agattagaac aagaagagac tgaaaaagcc ttactcgatt
    16981 tgcctcctgt tgatatggaa acgggtgaaa ttctgacaga ggaagctgtt caaaatcttc
    17041 cacctattcc agaagaaaag tgggtggaac cagaaatcat cctgcctcaa gctgaactta
    17101 aattccctga acaggaagat gactcagatg acgaagatgt tcaggtcgat ttttcagcca
    17161 aagaagccct tgaatacaaa cttccaagct tacaactctt tgcaccagat aaaccaaaag
    17221 atcagtctaa agagaagaaa attgtcagag aaaatatcaa aatcttagaa gcaacctttg
    17281 ctagctttgg tattaaggta acagttgaac gggccgaaat tgggccatca gtgaccaagt
    17341 atgaagtcaa gccggctgtt ggtgtaaggg tcaaccgcat ttccaatcta tcagatgacc
    17401 tcgctctagc cttggctgcc aaagatgtcc ggattgaagc accaatccct gggaaatccc
    17461 taatcggaat tgaagtgccc aactccgata ttgccactgt atctttccga gaactatggg
    17521 aacaatcgca aacgaaagca gaaaatttct tggaaattcc tttagggaag gctgttaatg
    17581 gaaccgcaag agcttttgac ctttctaaaa tgccccactt gctagttgca ggttcaacgg
    17641 gttcagggaa gtcagtagca gttaacggca ttattgctag cattctcatg aaggcgagac
    17701 cagatcaagt taaatttatg atggtcgatc ccaagatggt tgagttatct gtttacaatg
    17761 atattcccca cctcttgatt ccagtcgtga ccaatccacg caaagccagc aaggctctgc
    17821 aaaaggttgt ggatgaaatg gaaaaccgtt atgaactctt tgccaaggtg ggagttcgga
    17881 atattgcagg ttttaatgcc aaggtagaag agttcaattc ccagtctgag tacaagcaaa
    17941 ttccgctacc attcattgtc gtgattgtgg atgagttggc tgacctcatg atggtggcca
    18001 gcaaggaagt ggaagatgct atcatccgtc ttgggcagaa ggcgcgtgct gcaggtatcc
    18061 acatgattct tgcaactcag cgtccatctg ttgatgtcat ctctggtttg attaaggcca
    18121 atgttccatc tcgtgtagca tttgcggttt catcaggaac agactcccgt acgattttgg
    18181 atgaaaatgg agcagaaaaa cttcttggtc gaggagacat gctctttaaa ccaattgatg
    18241 aaaatcatcc agttcgtctc caaggctcct ttatctcgga tgacgatgtt gagcgcattg
    18301 tgaacttcat caagactcag gcagatgcag actacgatga gagttttgat ccaggtgagg
    18361 tttctgaaaa tgaaggagaa ttttcggatg gagatgctgg tggtgatccg ctttttgaag
    18421 aagctaagtc tttggttatc gaaacacaga aagccagtgc atccatgatt cagcgtcgtt
    18481 tgtcagttgg atttaaccgt gcgacccgtc tcatggaaga gcttgagatg gcaggtgtca
    18541 ttggtccagc tgaaggtacc aagccaagaa aagtattgca gcaataaaaa atagcttctt
    18601 tccaaggttg gaaggaagct atttttctgg ttattgatta cttttatttt ctgaagttgg
    18661 agtattgggc tgtttttcat tttcagtggc aggtttactt gtagcaggtg tagtagagtc
    18721 ctgagttgct gttttctgct cttctttttt ctcttccttg acgctagatt ttggcgtttc
    18781 ttcttgttgt gttttttctt gagaagtgtt attttcctta tttggagtag tattttcctg
    18841 aggggattct ttttgaattt ctttgacaaa ggttgtcgtc tggcttgtcg taggttcttt
    18901 tttggtattt ttattattat ccaaggcatt catgatcgtg atagtcgcag taatcaagcc
    18961 gaggatacta ccgatagtag taatggtttt ttgaaaaaca gtaaagcctt ttttgatatg
    19021 ttctgttttc ggttttgaag ttctacgata attagacatg cattctctcc tttgattatg
    19081 acttattata ctctgtttct ttaaaaaaat cttaaaaaaa gagaaattgc cctttcttgt
    19141 cgcaagctct gtttcatgat acaatagagg gagtgatttt agaaagcgtg agaaacatga
    19201 tttactttga taattcagcg acgaccaagc cttatcctga agcccttgaa acctatatgc
    19261 aggtcgcttc aaaaatttta ggaaacccat ctagtctcca tcgtttggga gaccaagcaa
    19321 cacgaatatt agatgcttct cgccaacaga ttgcagattt aatcggtaag aaaagcgatg
    19381 aaatcttctt tacctctggt ggaacagaag gggataactg ggttatcaag ggtgtggcct
    19441 ttgaaaaagc tcagtttggc aagcacatca ttgtttcagc cattgaacat ctagcagtca
    19501 aagagtcagc cctctggttg aaaagtcaag gatttgaagt ggattttgct cccgttgata
    19561 agaaaggctt tgtcgatgtt gaggcgttag cagatttgat acgtcctgat acgaccctcg
    19621 tttccatcgt ggcagttaac aatgaaattg gctctgttca gcccattgag gctatttcag
    19681 aattgttggc agatcagccg actatttctt tccacgttga tgcggttcag gcgcttgcca
    19741 agattccgac tgaaaagtat ctgacagaac gggtggattg cgcgactttc tctggtcaca
    19801 agttccacgg agttcgtggt gttggttttg tctatatcaa gtctggcaag aagattacgc
    19861 ctcttttaac aggtggtggt caggagcgcg actatcgttc gacaactgaa aatgtggcag
    19921 ggattgcagc gacagccaag gctctgcgtt tgtctatgga aaaactggat atctttagga
    19981 gtaagacagg gcagatgaag gcagtgattc gccaagctct tttagattat ccagatatat
    20041 ttatcttttc agctgaggaa gactttgccc ctcatatcct gacttttgga atcaagggtg
    20101 ttcgtggtga agtcatcgtt cacgcctttg aagactatga tattttcatc tcaacaacct
    20161 ctgcttgttc gtccaaggct gggaaacctg caggtacctt gattgccatg ggagtggata
    20221 aggataaggc ccagtcagct gtgcgtctta gtctagacct tgaaaatgat atgagtcagg
    20281 tcgagcagtt tttgaccaag ttaaaattga tttacaatca aactagaaaa gtaagatagg
    20341 agcattcatg cagtattcag aaattatgat tcgctacggt gagctgtcaa ccaagggtaa
    20401 aaaccgtatg cgtttcatca ataaacttcg caataatatt tcagacgttt tgtctatcta
    20461 tccccaagtt aaggtaaggg cagatcgcga ccgtgcccac gctcacctca atggagcaga
    20521 ttacacagca gtagcagaat cgctcaaaca agtatttgga attcagaatt tttctcctgt
    20581 ttataaggtt gaaaaatctg tagaagtttt gaagtcttct gtccaagaga ttatgcggga
    20641 catctacaag gaaggtatga cctttaagat ttctagcaag cgtagcgacc acaactttga
    20701 acttgatagt cgtgaactca accaaacact tggaggggct gtattcgaag ccattccaaa
    20761 tgtgcaagtt caaatgaaaa gtcctgacat caatcttcag gtggagattc gtgaagaagc
    20821 agcctatctt tcttatgaaa ccattcgtgg ggctggtggt ttgccagttg gaacttcagg
    20881 taaagggatg ctcatgttgt caggagggat tgactcacct gtagcaggtt atcttgctct
    20941 taagcgtggg gtggatatcg aggcagttca ctttgctagt ccaccatata ctagtcctgg
    21001 tgccctcaag aaagcgcagg acttgacccg taaattgacc aagtttggcg gaaatatcca
    21061 gtttattgag gtgcctttca cagagattca agaggaaatc aaagccaaag cgccagaagc
    21121 ttatttgatg actctaactc gtcgctttat gatgcggatt actgaccgta ttcgtgaggt
    21181 acgaaatggt ttggttatca tcaatgggga aagtctaggt caagtagcca gccaaaccct
    21241 tgaaagtatg aaggctatca atgctgttac caacactccc atcattcgtc ctgtggttac
    21301 catggacaag ttggaaatca ttgacatcgc ccaggaaatc gatacctttg acatttcaat
    21361 ccaaccgttt gaagactgtt gtaccatttt tgcaccagat cgtccaaaaa caaatcctaa
    21421 aattaagaat gcggagcagt acgaagcgcg tatggatgtt gaaggcttgg ttgagcgagc
    21481 agtggctgga atcatgatta ctgaaatcac acctcaagcc gaaaaagatg aagttgatga
    21541 cttgattgac aatctgctct aattcagaaa atccaaaaga atagcgaaaa tcagtaaaaa
    21601 aagttagttt tttctctaaa aacaggtaaa aaactaactt ttttctattt ttatgatata
    21661 atgatataaa attttgaata tagagagttt tctgacaatg aatcaatcct acttttatct
    21721 aaaaatgaaa gaacacaaac tcaaggttcc ttatacaggt aaggagcgcc gtgtacgtat
    21781 tcttcttcct aaagattatg agaaagatac agaccgttcc tatcctgttg tatattttca
    21841 tgacgggcaa aatgtttttt atagcaaaga gtctttcatt ggacattcat ggaagattat
    21901 cccagctatc aaacgaaatt cggatatcag tcgcatgatt gtcgttgcta ttgacaatga
    21961 tggtatgggg cggatgaatg agtatgcggc ttggaagttc caaaaatctc ctatcccagg
    22021 gcagcagttt ggtggtaagg gtgtggagta tgctgagttt gtcatggagg tggtcaagcc
    22081 ttttatcgat gagacctatc gtacaaaagc agactgccag catacggcta tgattggttc
    22141 ctcactagga ggcaatatta cccagtttat cggtttggaa taccaagacc aaattggttg
    22201 cttgggcgtt ttttcatctg caaactggct ccaccaagaa gcctttaacc gctatatcga
    22261 gtgccagaaa ctatcgcctg accagcgcat cttcatctat gtaggaacag aagaagcaga
    22321 tgatacagac aagaccttga tggatggcaa tatcaaacaa gcctacatcg actcgtcgct
    22381 ttgctattac catgatttga tagcaggggg agtacatctg gataatcttg tgctaaaagt
    22441 tcagtctggt gccatccata gtgaaatccc ttggtcagaa aatttaccag attgtctgag
    22501 attttttgca gaaaaatggt aagttaagaa agaaaaaaac gaaatgcata ttgaatatct
    22561 tagccactgg agtggtcatc ttaaccgtga aatgtacctt aaccgttatg gacatgctgg
    22621 gattccagtt gtggtctttg cttcatcagg tggtagtcac aacgaatact atgattttgg
    22681 catgattgat gcctgcactt cctttatcga ggaaggccgt gtccagttct ttaccctatc
    22741 tagtgtggat ggtgagagct ggttggctac ttggaaaaat gctcatgacc aagcggaaat
    22801 gcaccgttcc tacgaacgtt atgtgattga ggaggccatt ccttttatca agcacaagac
    22861 aggttggttt gatggcatga tgacgacagg ttgctctatg ggagcctatc atgcactcaa
    22921 tttcttcctc cagcatccag atgtctttac caaagtgatt gctctcagtg gtgtttacga
    22981 cgcacgtttc tttgtcggtg attactacaa cgatgatgct atttaccaaa actcgccagt
    23041 agattatatt tggaaccaaa acgacggctg gtttattgac cgttaccgtc aggcagagat
    23101 tgtgctgtgt acggggcttg gagcctggga acaagatggt ttgccatcct tttacaagct
    23161 caaagaagcc tttgacaaga aacaaattcc agcctggttt gctgaatggg gacatgatgt
    23221 cgcccatgac tgggaatggt ggcgtaaaca aatgccttat ttcctcggta atctctattt
    23281 ataaaaggag ttacctatga attaccttgt tatttctccc tactatccac aaaactttca
    23341 acagtttacc atcgaactag ctaataaagg catcacagtc ttgggaattg gtcaagagtc
    23401 ttacgagcaa ttggatgagc ccttgcgcaa tagcttgatc gagtattttc gtgttgataa
    23461 tcttgagaac atagatgaag tcaaacgtgc agttgctttt ctcttttata aacatggtcc
    23521 aattgaccgc atcgagtctc acaatgaata ctggcttgag ctagacgcaa cactcagaga
    23581 ataattcaat gtttttggtg ccaaaccaga ggatctcaaa aagacgaaat ataagtctga
    23641 aatgaagaaa cttttcaaaa aagcaggtgt tcctgtggta cctggagctg ttatcaagac
    23701 ggaagcagat gttgatcaag cagtgaagga aatcggtctt ccaatgattg ccaaacctga
    23761 taatggagtg ggagcagccg caacctttaa acttgagaca gaagacgata tcaatcactt
    23821 caagcaagaa tgggaccatt caacccttta tttctttgaa aaatttgtca cttccagcga
    23881 aatctgtacc tttgacgggc tcgtggacaa ggatggaaag attgtcttct caacaacctt
    23941 tgactacgcc tatacaccac ttgacctcat gatttataag atggacaatt cttattatgt
    24001 gctcaaggat atggatccta aactgcgcaa gtatggggaa gcaattgtca aagaatttgg
    24061 tatgaaagaa cggtttttcc atattgagtt cttccgtgag ggggacgatt atattaccat
    24121 cgagtacaat aaccgccctg caggtggttt taccattgat gtttataact ttgctcattc
    24181 cttgaacctt tatcgtggct atgcagctat tgtcgcagga gaggagttcc cagcgtcaga
    24241 ctttgaaact cagtattgtt tggctacttc tcgccgtgca aatgctcact atgtttattc
    24301 agaagaggat ttgcttgcca aatatagcca gcagttcaag gttaaaaaag tcatgccagc
    24361 tgccttcgcg gaacttcaag gagattacct gtatatgctg accactccga gtcgacaaga
    24421 aatggagcag atgattgcag atttcggaca acgtcaagaa taagaactat cggattaagg
    24481 aaattaactc ccttaatgct tttgttttgt ctgataaaaa ataagagcat cccaacaagg
    24541 tagctatcat aaaacttgtt cgataactat ttgaagcagg attaggtggt tagaaattga
    24601 attttaatat ttcaattgag tcatagtatt gtgttttcgt atccttaaat cagctaaaag
    24661 gatccatgac gacacctata cgatatagtt ttcaagatac caaacaagtc tattaatact
    24721 caatgaaaat caaagagcaa actaggaagc tagccgcagg tttctcaaaa cactgttttg
    24781 aggttgtgga tagaactgac agagtcagta tcatatacct acggcaaggt gaagctgacg
    24841 tggtttgaag agattttcga agagtataaa atattcaggt gacgcataga tatagttaat
    24901 tgaagctttg tttgaaatct gataaaataa tgatattact aagttttaaa aactaaagaa
    24961 aagggaagat atgattacag gcgaattaaa aaataaaatc gatcagttgt gggaaattct
    25021 ttggacagaa ggaaatgcaa atcctttaac aaatattgaa cagttgactt atctcttatt
    25081 tatgaaagat ttggatactg tcgagcttgg acgtgaaagt gatgctgaat ttctagggat
    25141 tccttatgag ggagtttttc caaaagataa acctgaatac cgttggtcaa cttttaaaaa
    25201 cataggagat gctcaggaag tttatcgttt aatgactcag gagatttttc cgtttattaa
    25261 aaatctcaag ggggatacag atgatacagc cttttcacga tatatgcgag aagctatttt
    25321 tcaaataaat aaacctgcta cgcttcaaaa ggcaatttct atcttagatg tttttccaac
    25381 taggggatta gatgtagatt ttgataatga caaacaaagt attactgata tcggagatat
    25441 ctatgaatat ctgttatcaa aattgtcgac cgcaggtaaa aatggacagt tccgtacacc
    25501 tcgtcacatc atcgatatga tggttgagtt gatgcaacca actatcaaag atatcatctc
    25561 agatcccgct atgggttctg ctggcttctt agtatccgct agccgttact taaagcgtaa
    25621 gaaagatgaa tgggaaacca atacagataa tatcaatcat tttcataatc agatgtttca
    25681 tggaaatgat acggatacga ctatgttgag acttggggcg atgaacatga tgctacatgg
    25741 agtagaaaac ccacaaatca gttaccttga ctcgctgtct caagataatg aagaagccga
    25801 taaatatact ttggttttaa caaatcctcc ttttaagggc tcacttgact acaattcaac
    25861 ctataatgac cttcttgcaa ccgtaaaaac caaaaaacag aattactctt tctttctctt
    25921 ttcttgcgaa ctttaaaacc aggtggacga gcagcagtta tcgtacctga tggtgtcctt
    25981 tttggttcgt ctaaagctca taaaggaatt cgtcaggaaa ttgtagagaa ccataagatt
    26041 aatgctataa tctcaatgcc tagtggtgtt ttcaagcctt atgctggagt ttcaactgcc
    26101 attctcatct ttacaaaaac tggcaatggt ggtaccgaca aggtctggtt ttacgatatg
    26161 aaagcggatg gtttgagttt ggatgataaa tgacaaccaa ttagtgacaa tgatattcca
    26221 gatattatcg aacgctttca tcatcttgaa aaagaagtag aacgtcagag aacggatcaa
    26281 tctttctttg ttccagttgc tgagataaag gaaaatgatt atgatttgtc tatcaataaa
    26341 tataaagaga ttgagtatga aaaagttgag tatgaaccaa cagaagtcat attaaagaaa
    26401 atcaatgatt tagaaaaaga aattcaagct ggcttggctg aattggaaaa attactcaag
    26461 tagggaggtg gctgtatgaa aaaagtgaag ttgggggaag tcttatctct aaaaaaaggc
    26521 aagaaagcca ctgtacttgc tgaacaaaca actctaagcc aacgttatat tcaaatagat
    26581 gatttaagaa ataataataa tttaaaattc actgaaagtt taaatatgac tgaagcactc
    26641 ccagatgata ttctgatagc atgggatgga gctaatgcag gaacagttgg ttatggatta
    26701 tcgggagctg ttggtagtac aattacggtc ttaaaaaaga atgagcgata caaagaaaaa
    26761 attatatcag attacttggg agtctttttg gaaagtaaat ccagttttct tatggaagct
    26821 tattacaaat gagaaaacaa aattaaaaat taagagaatt tcctctggaa ctagtgggag
    26881 tatgaaaaat atttcgaaat cacaattgtt acaaattaga gttcccttcc ccccactagc
    26941 cctccaaaat gagttcgcag actttgtatc cctggtcgac aaatcacaat tggcaatcca
    27001 aaaatctctg gaagaacttg aaactttgaa gaaatctctg atgcaggagt attttggctg
    27061 atattctgcc attgtaatta cggtaatgat ttgttataat acttcaaagg aggaaatcag
    27121 atggtagtaa aaacaagaaa acaaggaaat tcaatcacca ttacgattcc aagtgaattt
    27181 aatattccaa gtggtgttaa atacgaagcg aaattgttac caagtggtga gattatcttt
    27241 actcctgaag aattggggca gcaggtttct tatgtatctg atgatgcctt tgacttaaat
    27301 ttagataaaa tatttgacga atacgacgat gttttcaaag ctttggtgga aaaatgacaa
    27361 tctatctgac agaaaagcaa attgaaaaaa taaattcttt agcaattcaa cggtattctc
    27421 caaatgagaa aattcaaaca gttagtcctt ctgccttaaa tatgattgtg aacttaccag
    27481 aacaatttgt ctttgggaag cctctttatc caacaatttt tgataaagca acgatactat
    27541 ttgtccaatt gataaagaag catgtttttg ctaatgctaa taaaagaact gctttcttcg
    27601 ttttggtcaa atttttacaa ttaaacggct atcgtttttc tgtaacggta gaagaagcag
    27661 taaaaatgtg tgtaaccatc gcagtagaag ctttaactga tgaaaaaatg acaagctact
    27721 ccaaatgggt ttctgaacat tctgttagag aaaaggtcaa aaagtaacct agtatgctgg
    27781 atttgaatga gcacaagaaa ataaatgaac agacaatatt agaattctgt aatgcagaaa
    27841 ctgatattgt ctctttttat tgatgaataa gaaagtgaga aattatggaa tcaaaagtta
    27901 caattatcat gcaagaaatg ttacctcttt taaataatga acaattacta gcgttgagag
    27961 agagtttaga acatcatcta gtagacggaa aaaagcagca gaagtattcg aataataacc
    28021 tgttgcaact atttattacc gccaagcagg tagagggctg tagctcaaaa acaattcgtt
    28081 attaccagag gacgattgaa aacttgttta atgctattaa agagtctgtg acacaactca
    28141 caacagatga tttaaggagt tatttagcaa attaccagtc tgaaaaggat tgtagtaagg
    28201 caaacttaga caatattagg cgtatattgt cttctttttt tgcttggctt gagcaagagg
    28261 aatatatcat taaaaatccc attcgacgga taaagaaaat taagactgag caaaatgtga
    28321 aggaaactta tactgatgaa catttggaaa ttatgcgtga taactgtgaa aatttgagag
    28381 atttggcaat aatagaccta ctagtatcgt gtaggggagc ttgtacagtt gaatcgttca
    28441 gatattgatt ttgaaaacag agagtgtgtt gtctttggta aaggaaagaa ggagagacca
    28501 gtatattttg acgctcggac gaaaattcat ttaagaaatt atcttaacga cagaaaagat
    28561 agtcaccctg ctctttttgt aacgctagtt ggaaaagccc agagacttgg aattgctggt
    28621 gtagagattc gcttaagaaa attaggagac aaactcggca tacaaaaggt tcacccacat
    28681 aagttcagaa gaactttagc gactaaggca attgataaag gtatgcctat cgaacaagtc
    28741 caaaaactgc taggtcatag caagattgac acaaccctgg cctatgccat ggtcaatcaa
    28801 aataatgtca agcattcaca ccaaaaattc atctcttaaa agcaaatccc gatattccga
    28861 ggaggtggtt gcatgaaaaa agtgaaatta gggcaagtgg ctacttttat caatggctat
    28921 gcttttaaac ctcaagattg gtcatctgaa gggaaagaga ttatcagaat acagaatctg
    28981 actaaaactt caaaagggat aaattattat tcgggtacga ttgataaaaa atacattgtt
    29041 gaagctggtg atattcttat ttcgtggtcg ggtacattag gggtttttca gtggtgtgga
    29101 agatccgcag tgttaaatca gcacattttt aaagtggttt ttgataagat agatatagat
    29161 aaatcgtatt ttaaatatgt tgttgaaaaa ggtttgcaag atgctgtaaa acatactcat
    29221 ggttccacaa tgaaacattt aactaaaaaa tattttgata atattatggt ttcatataca
    29281 aatttgagag agcaacaaag aattgcaagt gagctggatt tgttaagcaa gctgatttta
    29341 aggagacaag aacaacttga agagcttaat ctacttgtaa aatcccgatt taacgagatg
    29401 tttggggaaa ataaaatatt tgaaagcatt gataacttat ttgatattat agatggtgat
    29461 aggggcaaaa attatcctaa atcagatgag ttgtttagtg aggagtactc gggactttga
    29521 cagttgtgaa g
//
LOCUS       DAVKDZ010000020        28598 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000020 DAVKDZ010000000
VERSION     DAVKDZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28598
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.020"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            273..2042
                     /locus_tag="VWZ30_000878"
     CDS             273..2042
                     /locus_tag="VWZ30_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEU9334643.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGSLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPIANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMLIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGASPIAEIEGDISTSASVDSQEIDFNHIDAVVVAYGKAQ
                     GSATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQTAPNS
                     QFLIVDSLVTTPEYDKMAVRMYK"
     gene            2066..4021
                     /locus_tag="VWZ30_000879"
     CDS             2066..4021
                     /locus_tag="VWZ30_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9334644.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECMKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQAYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            4023..4460
                     /locus_tag="VWZ30_000880"
     CDS             4023..4460
                     /locus_tag="VWZ30_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9334645.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            4522..5658
                     /locus_tag="VWZ30_000881"
     CDS             4522..5658
                     /locus_tag="VWZ30_000881"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEU9334646.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKSGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLITKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDPELIEQIVEYI"
     gene            5910..6959
                     /locus_tag="VWZ30_000882"
                     /pseudo
     CDS             5910..6959
                     /locus_tag="VWZ30_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
     gene            complement(7059..10829)
                     /gene="spuA"
                     /locus_tag="VWZ30_000883"
     CDS             complement(7059..10829)
                     /gene="spuA"
                     /locus_tag="VWZ30_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEU9334647.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDATSIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEVHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGPVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSVKEAVRNESV
                     ENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNKKEN"
     gene            11116..12462
                     /locus_tag="VWZ30_000884"
     CDS             11116..12462
                     /locus_tag="VWZ30_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HEU9334648.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDVISLVANMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFRHLFLH
                     VVGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPGRKASLMMH"
     gene            12954..13367
                     /gene="rpsL"
                     /locus_tag="VWZ30_000885"
     CDS             12954..13367
                     /gene="rpsL"
                     /locus_tag="VWZ30_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU9334649.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            13387..13857
                     /gene="rpsG"
                     /locus_tag="VWZ30_000886"
     CDS             13387..13857
                     /gene="rpsG"
                     /locus_tag="VWZ30_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU9334650.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            14282..16363
                     /gene="fusA"
                     /locus_tag="VWZ30_000887"
     CDS             14282..16363
                     /gene="fusA"
                     /locus_tag="VWZ30_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU9334651.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            16467..20858
                     /locus_tag="VWZ30_000888"
     CDS             16467..20858
                     /locus_tag="VWZ30_000888"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEU9334652.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     GAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNEIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNDYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            20960..21223
                     /locus_tag="VWZ30_000889"
     CDS             20960..21223
                     /locus_tag="VWZ30_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEU9334653.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            21216..21494
                     /locus_tag="VWZ30_000890"
     CDS             21216..21494
                     /locus_tag="VWZ30_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEU9334654.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            21515..21667
                     /locus_tag="VWZ30_000891"
     CDS             21515..21667
                     /locus_tag="VWZ30_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334655.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            21689..22930
                     /locus_tag="VWZ30_000892"
     CDS             21689..22930
                     /locus_tag="VWZ30_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU9334656.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            22940..23170
                     /locus_tag="VWZ30_000893"
     CDS             22940..23170
                     /locus_tag="VWZ30_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9334657.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            23438..24160
                     /locus_tag="VWZ30_000894"
     CDS             23438..24160
                     /locus_tag="VWZ30_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9334658.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            24377..25711
                     /gene="pepC"
                     /locus_tag="VWZ30_000895"
     CDS             24377..25711
                     /gene="pepC"
                     /locus_tag="VWZ30_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEU9334659.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAK"
     gene            complement(25767..26678)
                     /locus_tag="VWZ30_000896"
     CDS             complement(25767..26678)
                     /locus_tag="VWZ30_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9334660.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(26702..27505)
                     /locus_tag="VWZ30_000897"
     CDS             complement(26702..27505)
                     /locus_tag="VWZ30_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEU9334661.1"
                     /translation="MSIISMVLVIVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAVIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(27533..28522)
                     /gene="manX"
                     /locus_tag="VWZ30_000898"
     CDS             complement(27533..28522)
                     /gene="manX"
                     /locus_tag="VWZ30_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEU9334662.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
BASE COUNT         8286 a         5463 c         6457 g         8392 t
ORIGIN      
        1 aattgatttg ttcgtatctt atttcaaact actataaaaa gaggaagctg ggcaaaaact
       61 caatttgaag agaaaattca gtaaatcttt ccatgataaa acgcatcata ttaaggtttt
      121 gtacacctga tatgatgcgt tttatcattt ttaagccttt ttgcccagcc tcgtcaaaag
      181 taaagttttg gcacaaaatc tgtgacaaaa ctttagtttt aaaggttttt aactttgtat
      241 atactagttt taagaaaagg aggatgatcc taatggaaga aaaagtatca ttgaaagtca
      301 gagttcaaaa actagggaca tcgctttcaa atatggttat gcccaatatt ggagcattta
      361 ttgcttgggg agtgttgact gccctcttta tcgctgatgg ctatctgcca aatgaacagt
      421 tagctactgt tgttggtcct atgttaacgt atttattgcc aatcctgatt ggttacacag
      481 gtggatatat gatccatggt caacgtggtg ccgttgtagg agctattgct actgttggtg
      541 caatcacagg ttctagtgtt cctatgttta tcggagctat ggtaatgggc tcactgggag
      601 gatggactat caagaaattt gatgaaaagt tccaggaaaa aattcgtccc ggatttgaaa
      661 tgttagttaa taatttctca gctggtctcg ttggttttgc attattgctt ttggctttct
      721 acgcaatcgg tccagtcgta tcgactctta ctggagctgt tgggaatggt gttgaggcta
      781 ttgttaatgc tcgcctcctt cctatagcta atattatcat cgaaccggct aaagtccttt
      841 tcctcaataa tgccctcaat catggcattt ttactcctct gggagtagaa caggtagctc
      901 aagctggtaa gtcaattctc ttcctattgg aagctaatcc tggaccaggc ctgggaattc
      961 tattagctta tgctgtattc ggtaaaggtt ctgctaaatc ttcttcttgg ggggcaatgc
     1021 ttattcattt cttcggaggg attcatgaaa tttactttcc ttatgttatg atgaagccta
     1081 ctctattttt agctgctatg gcaggaggta tctctggaac ttttactttt caactcttag
     1141 acgctggtct taaatctcca gcttcaccag gttctattat tgcgattatg gctacggcgc
     1201 caaaaggcgt ttggccccat ctaaatgttc ttctaggtgt tttagtggca gcagttgttt
     1261 ctttccttgt agcagccctt attcttcatg cagacaagtc aactgaggat tcgctcgaag
     1321 ctgctcaggc ggctacccaa gcagctaagg ctcagtctaa aggtcagtta gtatcaactt
     1381 ctgttgatgc agttgtttcg acagactcag tggaaaaaat cattttcgcc tgcgatgctg
     1441 gtatgggaag ctctgctatg ggagctagta ttcttcgaga taaggttaaa aaagcaggtc
     1501 tagagattcc agtatctaat caggcaatct caaatttgct tgatacacca aaaacattaa
     1561 ttgttactca ggaagaactg acaccaagag ctaaagacaa gagtccaagt gctattcatg
     1621 tttctgttga taatttctta gcgtcccctc gttatgatga aattgtagct tcattaacag
     1681 gagcttctcc aatagcagaa attgaaggag atatatcaac ttcagcatca gtagatagtc
     1741 aggaaattga ctttaaccat attgatgctg tagtagttgc ttatggtaaa gcacagggat
     1801 ctgcaactat gggctgtgaa acgattcggg ctatctttag aaacaagaat attcgtattc
     1861 cagtttctac tgccaaaatt tcagaattag gtgaatttaa ttctaaaaac ataatgattg
     1921 taacaactat ttctttacag gcagaagtgc agcaaacagc accgaattct caatttctta
     1981 ttgtggatag tttagtaaca acaccagaat atgacaaaat ggctgttaga atgtacaaat
     2041 agaactagag gtttctaaat tacgaatgct attaaccaaa cgagaagaac aattattgaa
     2101 ggctttccta catgtaggga agctttcaat gcaagacatg actgaaatct tacaggtttc
     2161 atctagaaca atttatcgaa ctttatcaga tttgacagat agcatggagc aatatggaat
     2221 cgaaataacg aagcatggga aatactatat tttgactgga gagttggatg atttgccgac
     2281 agaacttgaa gtgttagttg agtatagtcc ccaagaaaga caagagttga ttacctatcg
     2341 ccttctgact gagagtggtt ttgtcaccaa tgaagcattg caagagtgca tgaaagtcag
     2401 taatgtaact attattcagg atatttcaga tattgataag cgtcttttag actttgatct
     2461 gaaaattgaa cgacaaaaag gttatcgtat ttctggtgat tcagttggta agagaagatt
     2521 tttggctatt ttactgacaa actgtatctc agtagcagat ttttcaaccg gtaattttgg
     2581 gagctttgat attttagaag cagatagaac taggctggcc agtcagattg ttaataagca
     2641 actgtcaggt tttccagata tggatgctag gatgaagatg ttttttgcga tcttgttatc
     2701 tcttataggt caggagcaaa acattgaaaa ttcacctaat actagtaagc aggctttgga
     2761 aatttctcaa aaaatttttc aagcttactc taagcagact gcacaatttt atagtattca
     2821 ggaaattatc tattttgcga gcatcttaga tgaattaatc attaaacgtc aggacaatcc
     2881 gctctttacg gagaaatttg atggtgaatt tttctacaat atttcaaatc tgattgatac
     2941 ggtttccatg tataccaaga ttgacttttt taaggacaag gttttattca attttctttt
     3001 ccatcatatt cggctcagtt taggcgtccc tatccttttt cagggtgaaa atttgccaga
     3061 atctgtccag attttagttg aaaggaataa atttctttat acagtcatca gtcttttagt
     3121 gaatgatatt tttccgaaat atcttcatac agagtatgag tatggcatga ttgccctaca
     3181 ttttatctct agcttaggcc gtagtccaga gatttatcca gtccgtgttt tgcttttaac
     3241 ggatgaacgt cgggtcacta gagatttatt agtcagtaaa attaagagtg ttgctccttt
     3301 tgtagagttg atagatattc agtctctagt agattaccac agtattgatc tcagtcagta
     3361 tgattatatt ttatctacca agccgctgac taatcaggaa atcgatgtaa tttctagttt
     3421 tccaaccgtc aaagaactgc ttgaattaca ggaacgactt cagtatgtac aggcacatcg
     3481 tacaattgtc gcgcgtgatg ctatcgctcc agagaaaagt tatgacttgc aagattattt
     3541 aatatctagt agtcagcttt tgagtcaatt cgagttggtt caattggaga ataatcaatc
     3601 atttgagcac acggtagaac aaatcatcca atatcagaag aatgtgagtg acagagatta
     3661 cctaacaaga aaattgttat ctcacttcca gaatagtcct atggctattc ctaatactgg
     3721 tctggtgctt ttacatagtc agtctagcaa agtaacaaca aatagtttta ctatgtttga
     3781 actcaaacta cctatctccg cattgtcaat gaaacgagag gaagaagagg tcaaaaggtg
     3841 tctgctaatg ctaatgtcta aagaagctag cgaggaagcg agagatttaa tgacagctat
     3901 tagtcagtcg attattgaaa atcatcttta tacagagatt tacaagacgg gaaatcaatc
     3961 cattatttat cagatgctaa atactatttt taacgaaaaa attaagaaat tggagaacta
     4021 atatgaaact tgaaaaacat ttgattaagc ttaataaaca attttctaac aaggaggaag
     4081 ctatttgtta ttgtgggcaa gttctttatg agggtggata tgttaatgaa gactatattg
     4141 aagccatgat tgagcgagat aaagagctat ctgtttacat gggtaacttt atcgccatac
     4201 cgcatggaac agatgcagca aaaaaggatg tcctcaagtc tggtattaca gtcgttcaag
     4261 tccctagagg ggttgatttt gggaatgtat ctaaccctca agtggcaacg gttctttttg
     4321 gtattgctgg tattggtaat gaacacttag aaattattca gaaaatttct atcttctgtg
     4381 cagatgtaga taatgttctt aaactagcag atgctcagtc aaaagaggaa gtattgcgct
     4441 tatttgatgc tgttgaataa ttgaatttcg tcatttgtca tctagtatat atgtccctca
     4501 aataggaaaa ggagaaattg aatgaaacat tctgttcatt ttggtgccgg taatatcggt
     4561 cgtggtttta taggtgaaat tctatttaaa agtggtttcc atattgattt tgtggatgtc
     4621 aataatcaga taattcatgc tctaaatgaa aagggcaagt atgaaattga aattgcacag
     4681 aaaggacagt ctcgtataga agtaactaat gtggctggca ttaatagcaa agaacatcct
     4741 gagcaagtca ttgaagcgat tcaaaagacg gatattatta ctactgcaat cggacctaat
     4801 atactccctt ttatcgccga acttctagcc aaaggaatcg aagctcgccg agttgcagga
     4861 aatacacagg cattggatgt tatggcctgt gaaaatatga ttggcgggtc tcaatttctt
     4921 tatcaagaag tcaagaaata tttaagtccg gaaggtttga catttgctga taactacata
     4981 ggttttccaa atgctgcagt agacaggatt gttccagcac aaagtcacga agattccctt
     5041 tttgttgtgg tcgaaccctt taatgaatgg gtcgtggaaa ccaagcgtct taaaaatcca
     5101 gatttacgtc taaaagatgt gcattatgaa gaagatttag aaccctttat tgagcgaaaa
     5161 cttttttcag tcaattctgg acatgcaact tcagcttaca ttggtgcgca ttatggtgcc
     5221 aagacaattt tggaagctct tcaaaatcct aatatcaaat ctcggattga atctgtatta
     5281 gctgaaattc ggagtctctt gattaccaaa tggaactttg ataaaaaaga attggagaat
     5341 tatcacaaag tcattataga acggtttgaa aatcctttca tagtggacga ggttagtcgc
     5401 gtagctcgta ctccaatccg aaaattaggc tataatgaac gattcatccg gccgatacgt
     5461 gaattgaaag aactcagttt gtcatataaa aacctactta aaacagttgg ctatgccttt
     5521 gactatcgcg atgtaaatga tgaagaaagt attcgattag gtgaattgtt ggctaaacaa
     5581 tcagtcaaag atgttgttat acaagttaca ggtttagacg acccagaatt gattgagcaa
     5641 attgtagagt atatttaatc tttttcgaaa atctcttcaa atcaggttag catccatctg
     5701 caacctcaaa acagtatttt gagctgactt catcagttct atctaccacc tcaaagcagt
     5761 gctttgagct gactccgtca gtcttatctg caacctcaaa acagtgcttt gagcgcctgc
     5821 ggctagcttc ctagtttgct ctttgatttt cattgagtgt tagaataatt tttcaaattg
     5881 aaggacagag atagacaagg agaatcacaa tggtagaatt gggaatttca acatttgggg
     5941 aaataacgga gcttgaaggg actggacaaa cttacagtca tgctgaacgc attcgccagt
     6001 tggtggcaga gattgagctg gctgacaagg ttggtttgga cgtatatggg attggcgagc
     6061 accatcgggc ggattttgca gtatcagtcc cagagattgt cctggccgct ggggcagtca
     6121 atatcaagaa aatccgtttg accagtgcag tcagcattct gtcaagcatg gctctgattc
     6181 gtttgttcca acagtatgcc actatcgatg ctttgtcaaa tggacgatcg gagattatgg
     6241 ctggaagggg ctctttcacg gaatctttcc ctctgtttgg ttatgacttg aaagactacg
     6301 actctctttt tgatgagaaa ttagacttgc ttcagttagt caatgaaaag accaagttag
     6361 actggcaagg tcgattgact caaacgattg ctggtaaaga agtttatcct cgtccagttt
     6421 aggacaaatt gcccttgtgg atagctacag gtggtcatgt cgaatcaaca gtgaagattg
     6481 ctcaggctgg tctaccgatt gtatatgcta ttattggtgg taatccacgt tattttaaaa
     6541 agttgattca ggcttatcgt gagattggaa gcgaagcggg ccatgctgac aaagacttga
     6601 aagtgggagc ccattcttgg gggtggattg cggaagatgg tgagcaggcg gtgaaagatt
     6661 atttccatcc gactaagcaa gtggtggatg ctatttccaa agaccgtccg cactggcagg
     6721 aattgcgtta tgagcaatat ttggagcagg tagggccaaa tggcgccatg tttgttggca
     6781 atccagatca ggtggccaaa aaattgattc gcatgataga ggatttagga ttggaccgct
     6841 tcatgcttca tctaccgctt ggttctatgc ctcatgacca ggttctgaga gctattgaac
     6901 tcttcggaac acaagttgca cccaaagtac gagcttattt tgctatgaaa gaggcttaat
     6961 aaaaaatcct aatcaagttt attaggacaa caaatattgt acaaaaatta atcccccgag
     7021 ctataatgct tgggggattt ttctatagga gggctagttt agttctcttt tttatttttt
     7081 agtaagaaac cgagaccaag gagcgcaagg aggctgattc ctgcaaatag gagtttgttt
     7141 tcgtttttga ttcctgtatt tggaagttca gcttgtttat ctggggttgc aggtatattt
     7201 tccttgctag agttttctac cgattcgttt cgaaccgctt cttttacaga tgatgcttgt
     7261 gcttcttgtg ctggttgttc agcttgtgaa tcagctgtag cttggctagg tttattttcc
     7321 gcatcagcta ctttggcatc tggtttagta gaatcaggtc tagcttctgg agctggatct
     7381 tgatgttcag gcttggaagg ggttgagtct ggtttctctt ctgcagttga ctcatggcta
     7441 gttccgtttt gagagactcg aagaacagta gctgtaaggg cattcaattt caagcctttt
     7501 tcagtccatt caagtccttt cgggttggca attccgactg gtcctgcttg gttttcatct
     7561 gccaaaactt ccgcatttct tagatgtgca aaggcagttc ccaaattaaa ttcgcgagct
     7621 ttttcatccg cattgacaaa gactgcgtag atatcgccgt ttggagcagt gatttggtag
     7681 ccaatcacta catcctcttt ttccacacca ttttggcctg ggacagtgat gaggtggaca
     7741 cggtctttga tatcttgaag actcttaagt cggaaggcat ctgtagattg acgaagggca
     7801 atcaaacctt tcatatagtc acggctcttg acattttcag gataagcttt accatctgta
     7861 gccttagtcc agtcaaactt gttgactgca tcgctagaat cgtaagaatc atggatgaag
     7921 taaggatagt caaatgggtt gccgtcctta tcacgcaaca agtgagattt gtttggaacc
     7981 ttatcctctg ctactggagt cttgtaggct gggtcacgga attgtttagt acgtccatat
     8041 tcctgaccgg agtggataaa tggagttcct tgagctgtca agaccatgag atttccaagt
     8101 cgtaaacgac ggtggacttc agcatagttc tcagccttgc ttgggtcttt tttgatagac
     8161 tgggcaatga tgtcaaagag ggtcaagtta tcatgggctg cgatgtattg gatgacatct
     8221 ccagggctgt cagcttcaaa gttagttggt tgagcaatga gatttttaaa gatggtgttg
     8281 acatcacgct tgccacctgt gataaaggca ggttgacctt cgtttggata accagatttg
     8341 aggttgttac ggatgtcatc tgaaaagaca gcgacagtat cggtatgttt catccaatct
     8401 tggtcagcag ctttagtagg catgttttca tcaccggcat aggttctcca accttcacca
     8461 agcatgatga ggtttggatt gagggcgcgt gcagccttgt aagcttcttc gatagaagtg
     8521 gcgtcatggt ctcccatcat atcgaagcgg aagccatcca ctttgtaggt atccactagg
     8581 tatttgatag agtcaactag gagccgtttg gtcatatggt gggttgtccc caagcgtcca
     8641 ccaccaaagc tagttcgagg tgtgccatcg gcatccataa agtggtagta gtttggctct
     8701 aggtcttcaa agatatcgac tttggctgtg tggttataaa cgacatctag gatagctccc
     8761 ataccacgtt tgtggatttc gttgatgagg tttttaaatt ctgcgattcg tttttctgga
     8821 ttcttaggat cgcttgagta cataccagtc aatgagaagt agttttgagg gtcatatccc
     8881 cagttgtagt tgctgttgct tgaagcatag tcagacaagc gttcatggtt tttcaattca
     8941 ttgacaaagt agtaagacaa gactggaagg agctggatgt gggttacacc caagtctttg
     9001 aggtagtcta gtttttcgat aaaggcttca aaagtcccaa atggtttggt caagtctttt
     9061 gcaatggcag gatctgaagt gaaatcacgc acatgagctt cgtagataac ggcgtcttca
     9121 cgagtcttga aattgtgaat cttaccataa gtcaagtctt gaggtccgag tttagctgga
     9181 tctacaaagg cggctttagc cactttatgg gcatcgtcaa tcttggcatc gtcgctattc
     9241 caagcagcaa gagatttagc gtaaggatcg agtgcaagaa cagttttacc ttgacgctcg
     9301 atttggtatt gataatagta gccagtgaaa tctgtgattc cgagtttgtt tgtgctgtct
     9361 agagtttgtt tccaagttcc tctttcccct ttttcgagag cgacagttcc aactactttg
     9421 tcagggtcat tcttgtcgta gacaacaaca gaaaccttat cagcacttgg tgaccaaagg
     9481 gtcaaatcaa cttgttttcc ttcttctttt aggtcagctc ccagtttgcc atcatagctg
     9541 tatgtctcat ctttcaggcg ccagcttgtt ttggtagtga attggtcgga attgtagcta
     9601 acagtataag gatgttttgt gtcagagaaa tctccgctgt aggtcacttt cttaccagct
     9661 tcatcgattg caacatcggt aatagttacc ttgtttccta ggtgattagt gatgttggag
     9721 tgtttgagga tatcttcttt tttagcaccg acaagtgttg aaaagctact ttcaatgcta
     9781 gaagtgccta cgtgttgggc tcctgtcata cggatatcat ggacatagta tggatttgtg
     9841 taaatcgatt catcatcgtc ttttaggaaa atttggctat gatttttcaa atctgtgaac
     9901 ttataatttt ctttacggat tttcacgtcg tctccttgtt tgctctcatc tagtaataaa
     9961 aatccaaatt ctcttgcggc ttcattaaga ggaatgtcga tatagcggcc atatttgcct
    10021 gtagccgtaa agtctgttcc gtcaggccat tgagcgctac ttggattttt cacatctccc
    10081 cagtaccaga gagatttctt gtcatagttg ccatctgtgc ggtagtagtt gacgcgaaca
    10141 gttcctgcag gctgtggctc gtaagagaaa accttgtaat cttggtctaa ccaagcctcg
    10201 ttcatttttg gaactagttt ttctacagat ttatcgccgg ttagattttt tccagctgta
    10261 ttgttgatga ggaagctaat tttcttggct tgttctccct ttaatttgac atctaggtaa
    10321 tagccgtagt catctttctt ggcatccttg aaggacaaag ctccgtttgg ccagttttca
    10381 gatggttttt caacatcgtc ccaagtccat agtccttgag catccttgtt ttcttcagga
    10441 agttttttga catggatacg gaagtagtta tcttcgatgg gctcttctgc cttagtttca
    10501 gttgttactg gactagtaga agtagttggc tctgttgcag gtttttcttc tgttttctct
    10561 agggaagcgt ttgcatttct aggtgaagaa atatcacttt cgttcttacc agagccagct
    10621 gctgtatcag taagaggttg ggcaagggtg gttgtatttc tgttgttggg ttcgttagtg
    10681 gttggtgtac tttcgtcgga tgagatagtt ggtgtagcca tggcaagcaa gacaaggctt
    10741 gcgccgataa ggacagaacc agttccattt ttgagggaac ggatgctgta gaccattttt
    10801 ttctcagtgt gagatggtgt ttttctcata atgattctcc aatcaataaa tttctatatc
    10861 agtatagcat gtagtaaaac aatatgcaag cgttttcttt gatttgtgaa aaaaattctt
    10921 acttttgaga agttttctgt actattttaa ccccaattat tcaccccaaa tctaaaaacc
    10981 atccagaatc cttgccttag cttagatcct ggatggtttc ttttttcacc caatgggtgt
    11041 tttttactag acaaaaaaga gtttcccctt tatggtataa gtgtagaaaa aaacacaaaa
    11101 agaaaggaaa ctcacatgaa cagtttacca aatcatcact tccaaaacaa gtctttttat
    11161 caactatctt tcgatggagg tcatttaacc cagtatggtg gtcttatctt ttttcaggaa
    11221 cttttttccc agttgaaact aaaagagcgg atttctaagt atttagtaac gaatgaccaa
    11281 cgccgctact gtcgttattc ggattcagat atccttgtcc agttcctctt tcaactgtta
    11341 acaggctatg gaacggacta tgcttgtaaa gaattgtcag ctgatgccta ctttccaaaa
    11401 ttgttggaag gagggcagct tgcttcacag ccaaccttat cccgttttct ttccagaact
    11461 gacgaggaaa cagtccatag tttgcgatgc ctcaaccttg aattggtcga attcttttta
    11521 cagtttcacc agctaaacca actcattgta gatatcgatt ctacccattt cacaacttat
    11581 ggcaagcaag aaggtgttgc ttataacgcc cactatcgtg ctcatggcta tcatcctctt
    11641 tatgctttcg aggggaagac aggttattgt ttcaatgccc agcttcgtcc tggtaatcgt
    11701 tattgttctg aagaggcaga cagctttatc acacctgttt tagaacggtt taatcaactt
    11761 ctctttcgaa tggatagtgg ctttgcgacc ccaaaattat acgatttaat tgaaaaaaca
    11821 gggcaatact acctcataaa actcaagaaa aatactgttc tgagccgtct tggagacctt
    11881 tccctccctt gcccacagga tgaggactta accatcttgc cccactccgc ctactcagaa
    11941 actctctatc aagcaggatc ttggtcgcac aagcgtcgtg tctgccagtt ctctgaacga
    12001 aaagaaggaa acttgttcta cgatgttatt tctctcgttg caaatatgac gagtggaaca
    12061 agccaagacc agtttcagct ttatcgtgga cgtggtcaag ccgagaattt catcaaggag
    12121 atgaaggagg gattttttgg cgataaaacg gatagttcaa ccttaatcaa aaacgaagtt
    12181 cgtatgatga tgagctgtat cgcctacaat ctctatcttt ttctcaaaca tctagctgga
    12241 ggcgacttcc aaactttaac aatcaaacgc ttccgccatc tttttcttca cgtggtagga
    12301 aaatgtgttc gaacaggacg caagcagctc ctcaaattgt ctagtctcta tgcctattcc
    12361 gaattgtttt cagcacttta ttctaggatt agaaaagtca acctgaatct tcctgttcct
    12421 tatgaaccac ctggaagaaa agcgtcgtta atgatgcatt aaagaacagt cgagatgaaa
    12481 aaatcgtgtg acgcaccaag ggaggagtct gcccttttga ggaaatctag cgaggaaaaa
    12541 cgatactgga acagcagaaa gtaaaactga cctcatgagg aggaagaaag tggctcatga
    12601 ggtcaggggt tttgtaagtt acatctagtt gagagaggta tgaatgattt gggtttatat
    12661 atcagtataa atgacggaga taaaaatgtc acataagcca agccgtatta ttaggaagat
    12721 gatgtgttcc cagctatttt tattcagaaa ttgatctcta acattaccaa taaaaaagaa
    12781 aaatatcttg acaagcaagg gaaaattttg ttacaataat agacggtact ttttactttt
    12841 ggtctctcaa gagtgtacag ggacgtgctg acaaatgttg caaaagtaca cacagatgat
    12901 atctgtcacc aagtgtatca tcaccaaaaa taaaaaaaca caggagaatg tagatgccta
    12961 caattaacca attggttcgc aaaccgcgta aatcaaaagt agaaaaatct aaatcaccag
    13021 ctttgaacgt tggttacaat agtcataaaa aagttcaaac aaacgtttct tcaccacaaa
    13081 aacgtggtgt tgcaactcgt gttggaacaa tgacacctaa aaaacctaac tcagcccttc
    13141 gtaaattcgc tcgtgtacgt ttgagcaacc ttatcgaagt tactgcctac atcccaggta
    13201 tcggacacaa cttgcaagag cacagcgtgg tgcttcttcg cggtggacgt gtaaaagacc
    13261 ttccaggggt acgttaccat atcgtccgtg gtgcacttga tactgcaggt gttaacgatc
    13321 gtaaacaagg ccgttctaaa tacggtacta aacgtccaaa agcataagga aaggggataa
    13381 agagaaatga gtcgtaaaaa tagagctcca aaacgtgacg tattgccaga tccgctatac
    13441 aattcacaac tagttactcg tcttatcaac cgcgttatgc ttgatggtaa acgtggtact
    13501 gctgcttcaa tcgtttacgg tgcttttgag caaatcaaag aagctactgg caacgatgca
    13561 cttgaagtat ttgaaacagc tatggaaaac atcatgcctg tacttgaagt acgtgcacgt
    13621 cgtgttggtg gttctaacta ccaagtccca gttgaagttc gtccagaacg tcgtacaaca
    13681 cttggacttc gttggttggt aacaatcgct cgtcttcgtg gtgaacacac aatgcaagac
    13741 cgtcttgcaa aagaaatctt ggatgctgct aacaacactg gtgcagctgt taagaaacgt
    13801 gaagatactc accgtatggc tgaagctaac cgtgcattcg cacacttccg ttggtaagat
    13861 aggatgcgaa agcgttaaga aagtcccaga gaaaataggg aatcgaagca ggttgcggtt
    13921 gcaaccaatg agattcatct ttttctccag acttttagct tgagctcaac taaatcatga
    13981 tgctaggcac ggtaaggatg caaggtgaaa ataggaaact gacgcagtat tcaacgaata
    14041 caaggagttt tatctttttc acgcagcatc ccgttccagc tcacatcggc taactaactt
    14101 tagcccgggt tcaaattagc taaatcgatt agtattagct ataactcagc ttaccatcta
    14161 gtaagttgaa accaacaata gcatgaaaac attgagaacg ggtaggtcct gcctatccgt
    14221 ttttattaaa atcgtgttat aatagaatag aaatcaaaaa taaataggag aaacaaacct
    14281 catggcacgc gaattttcac ttgaaaaaac tcgtaatatc ggtatcatgg ctcacgtcga
    14341 tgccggtaaa acaacaacta ctgagcgtat tctttactac actggtaaaa tccacaaaat
    14401 cggtgaaact cacgaaggtg cgtcacaaat ggactggatg gagcaagagc aagaacgtgg
    14461 tatcacgatc acatctgctg cgacaacagc tcaatggaac aaccaccgcg taaacatcat
    14521 cgacacacca ggacacgtgg acttcacaat cgaagtacaa cgttctcttc gtgtattgga
    14581 tggtgcggtt accgttcttg actcacaatc aggtgttgaa cctcaaactg aaacagtttg
    14641 gcgtcaagca actgagtacg gagttccacg tatcgtattt gccaacaaaa tggacaaaat
    14701 cggtgctgac ttcctttact ctgtaagcac acttcacgat cgtcttcaag caaatgcaca
    14761 cccaatccaa ttgccaatcg gttctgaaga tgacttccgt ggtatcattg acttgatcaa
    14821 gatgaaagct gaaatctata ctaacgacct tggtacggat atccttgaag aagacatccc
    14881 agctgaatac cttgaccaag ctcaagaata ccgtgaaaaa ttgattgaag cagttgctga
    14941 aactgacgaa gaattgatga tgaaatacct cgaaggtgaa gaaatcacta acgaagaatt
    15001 gaaagctggt atccgtaaag cgactatcaa cgttgaattc tttccagtat tgtgtggttc
    15061 agccttcaaa aacaaaggtg ttcaattgat gcttgatgcg gttatcgact accttccaag
    15121 cccacttgac atcccagcaa tcaaaggtat taacccagat acagacgctg aagaaactcg
    15181 tccagcatct gacgaagagc catttgcagc tcttgccttc aagatcatga ctgacccatt
    15241 cgtaggtcgt ttgacattct tccgtgttta ctcaggtgtt cttcaatcag gttcatacgt
    15301 attgaatact tctaaaggta aacgtgaacg tatcggacgt atccttcaaa tgcacgctaa
    15361 cagccgtcaa gaaatcgaca ctgtttactc aggtgatatc gctgctgccg ttggtttgaa
    15421 agatactaca actggtgact cattgacaga tgaaaaatct aaaatcatcc ttgagtcaat
    15481 caacgttcca gaaccagtta tccaattgat ggttgagcca aaatctaaag ctgaccaaga
    15541 caagatgggt atcgcccttc aaaaattggc tgaagaagat ccaacattcc gcgttgaaac
    15601 aaacgttgaa actggtgaaa cagttatctc aggtatgggt gaacttcacc ttgacgtcct
    15661 tgttgatcgt atgcgtcgtg agttcaaagt tgaagcgaac gtaggtgcgc ctcaagtatc
    15721 ttaccgtgaa acattccgcg cttctactca agcacgtgga ttcttcaaac gtcagtctgg
    15781 tggtaaaggt caattcggtg atgtgtggat tgaatttact ccaaacgaag aaggtaaagg
    15841 attcgaattc gaaaacgcaa tcgtcggtgg tgtggttcct cgtgaattta tcccagcggt
    15901 tgaaaaaggt ttggtagaat ctatggctaa cggtgttctt gcaggttacc caatggttga
    15961 cgttaaagct aagctttatg atggttcata tcacgatgtc gactcatctg aaactgcctt
    16021 caagattgcg gcttcacttt cccttaaaga agctgctaaa tcagcacaac cagctatcct
    16081 tgaaccaatg atgcttgtaa caatcactgt tccagaagaa aaccttggtg atgttatggg
    16141 tcacgtaact gctcgtcgtg gacgtgtaga tggtatggaa gcacacggta acagccaaat
    16201 cgttcgtgct tacgttccac ttgctgaaat gttcggttac gcaacagttc ttcgttctgc
    16261 atctcaagga cgtggtacat tcatgatggt atttgaccac tacgaagatg tacctaagtc
    16321 agtacaagaa gaaattatta agaaaaataa aggtgaagac taatccgtcc tcactctaga
    16381 aggaagtcac ttagtggctt ccttttgtct ttagaaaata cctctaaata tggtaaaata
    16441 gtagaagaat aatgtgagga aaatgaatgt caaatagttt tgaaattttg atgaatcaat
    16501 tggggatgcc tgctgaaatg agacaggctc ctgctttagc acaggccaat attgagcgag
    16561 ttgtggttca taaaattagt aaggtatggg agtttcattt cgtattttct aatattttac
    16621 cgattgaaat ctttttagaa ttaaagaaag gtttgagcga agaattttct aagacaggca
    16681 ataaagctgt ttttgaaatt aaggctcggt ctcaagaatt ttcaaatcag ctcttgcagt
    16741 cctactatag ggaggctttc tctgaaggtc catgtgctag tcaaggtttt aagtcccttt
    16801 atcaaaattt gcaagttcgt gctgagggta atcagctatt tattgaagga tctgaggcga
    16861 ttgataagga acattttaag aagaatcatc ttcctaattt agccaaacaa cttgaaaagt
    16921 ttggttttcc aacttttaac tgtcaagtcg agaagaatga tgtcctgacc caagagcagg
    16981 aagaggcctt tcatgctgaa aatgagcaga ttgttcaagc tgccaatgag gaagcgctcc
    17041 gtgctatgga acaactggag cagatggcac ctcctccagc ggaagagaaa ccagtctttg
    17101 attttcaagc gaaaaaagct gcagctaaac ccaagctgga taaggcggag attactccta
    17161 tgatcgaagt gacgacagag gaaaatcgtc tggtatttga aggggttgtt tttgatgtgg
    17221 agcaaaaagt gactagaaca ggtcgtgttt taatcaactt taaaatgacg gactatactt
    17281 caagtttttc tatgcaaaag tgggttaaaa acgaggaaga ggcccagaag tttgacctca
    17341 tcaagaagaa ttcttggctc cgagttcgag ggaatgtgga gatgaataac ttcacacgcg
    17401 atttgactat gaacgtacag gatctgcagg aagttgttca ctatgagcgg aaggatttga
    17461 tgccagaagg tgagcgtcgg gttgagtttc atgctcatac taacatgtcg actatggatg
    17521 ctttgccaga ggtcgaagag attgttgcaa cagctgctaa gtggggacac aaggcggttg
    17581 ctatcacgga ccatgggaat gtccagtcct ttccacatgg ctataaggcg gctaagaaag
    17641 cgggaatcca gctgatctat ggaatggaag ccaatatcgt ggaggaccgt gtccctatcg
    17701 tctataacga agtggagatg gacttgtcag aagcaaccta cgtggtcttt gacgtggaaa
    17761 cgacgggact ttcagctatc tataatgact tgattcaggt tgcggcctct aagatgtaca
    17821 aggggaatgt tattgctgaa tttgatgaat ttatcaatcc tgggcatccc ttgtcagctt
    17881 ttactacaga gttaactgga attacagatg atcatgtcaa aaatgccaaa ccactagaac
    17941 aagttttgca agaattccaa gaattttgca aggatacggt cctagttgcc cacaatgcta
    18001 cctttgacgt tggctttatg aatgctaatt atgagcgtca tgatcttcca aagattagtc
    18061 agccagttat tgatacgctg gagtttgcta gaaacctcta tcctgagtat aaacgccatg
    18121 gtttggggcc tttgaccaag cgttttggtg tggccttgga acatcaccac atggccaact
    18181 acgatgcgga agcgactggt cgtctgcttt tcatctttat caaagaggta gcagaaaaac
    18241 atggtgtgac cgatttagct agactcaaca ttgatctaat cagtccagat tcttacaaaa
    18301 aagctcggat caagcatgcg accatctatg tcaagaatca ggtaggtcta aaaaatatct
    18361 ttaagctggt ttccttgtct aataccaagt attttgaagg agtgccacgg attccgagaa
    18421 cggttctaga tgcccatcga gagggcttga ttttaggttc agcctgttca gagggtgaag
    18481 tttttgacgt ggtcgtttct caaggtgtgg atggggcggt tgaggtggcc aagtattatg
    18541 actttatcga ggtcatgcca ccggctatct atgcgccctt gattgccaaa gagcaggtca
    18601 aggatatgga ggaactccag accattatca agagtttgat agaggttgga gatcgccttg
    18661 gcaagcctgt tctggctacg ggaaatgttc actatatcga accggaagaa gagatttatc
    18721 gtgaaattat tgtccgtagt ttgggacagg gtgcgatgat taatcgaact atcggtcatg
    18781 gtgaacatgc ccaaccagca ccacttccaa aggctcattt tcgaacgact aatgagatgt
    18841 tggatgaatt tgcctttttg ggagaggaac tggctcgtaa actggttatt gaaaacacta
    18901 atgccttggc agaaatattt gaacccgttg aagtcgttaa gggtgacttg tatacgcctt
    18961 tcatcgacaa ggctgaagaa acagttgctg agttgaccta taagaaagct tttgagattt
    19021 atggaaatcc gctgccagat attgttgatt tgcggattga aaaagaatta acatccatac
    19081 tggggaatgg atttgctgtg atttatctgg catcgcagat gctggtgcaa cgttctaatg
    19141 aacggggtta tttggttggt tctcgtgggt ctgtcggatc tagtttcgtt gcgaccatga
    19201 ttgggattac ggaggtcaat cctctctctc ctcactatgt ctgtggtcag tgtcagtaca
    19261 gtgagtttat cacagatggt tcgtacggtt caggatttga tatgccccat aaggactgtc
    19321 caaactgtgg tcacaaactc agtaaaaacg gacaggatat tccgtttgag accttccttg
    19381 gctttgatgg ggataaggtt cctgatattg acttgaactt ctcgggagaa gatcagccta
    19441 gcgcccactt ggatgtgcgt gatatctttg gtgaagaata tgccttccgt gcgggaacgg
    19501 ttggtacggt agctgccaag actgcctatg gatttgtcaa gggttacgag cgagattatg
    19561 gcaagtttta tcgtgatgca gaagtagaac gcctcgctca aggagcggcg ggtgtcaagc
    19621 ggacaacagg ccaacacccg gggggaatcg ttgttattcc gaactacatg gatgtctacg
    19681 attttacgcc tgtccagtat ccagcagatg atgtcacggc tgaatggcag accactcact
    19741 ttaacttcca cgatatcgat gagaacgtcc tcaaactcga tgtactggga catgatgatc
    19801 cgaccatgat tcgaaaactt caggatttgt ctggtattga ccctaatgaa attcctatgg
    19861 atgacgaagg cgtgatggca ctcttttctg ggactgatgt gctaggggta acacctgaac
    19921 aaattggaac gcctacgggt atgttgggga ttccagagtt tggaacaaat ttcgtacgtg
    19981 gaatggtaga cgaaacccat ccgacaacct ttgcggaatt gcttcagctg tctggtctgt
    20041 cccacggtac tgacgtttgg ttggggaatg ctcaggatct gattaagcaa ggaatagcgg
    20101 acctatcgac tgttatcggt tgtcgggacg acatcatggt ttacctcatg catgcgggtc
    20161 tggaacctaa gatggccttt accattatgg aacgggtacg taagggtttg tggctaaaga
    20221 tttcagaaga ggagagaaat gactatatcg aagccatgaa ggccaataag gtgccagagt
    20281 ggtatatcga atcctgtggg aaaattaagt acatgttccc taaggcccat gcggcagcct
    20341 acgttatgat ggccttgcgt gtagcttact tcaaggttca ccatcctatt tattactact
    20401 gtgcttactt ctccattcgt gctaaggctt ttgatatcaa gaccatgggt gcgggcttgg
    20461 aggtcatcaa gcgcagaatg gaagaaatct ctgaaaaacg gaagaacaat gaagcctcta
    20521 atgtggaaat cgatctctat acaactcttg agattgtcaa tgagatgtgg gaacgaggtt
    20581 tcaagtttgg taaattagat ctctaccgta gtcaggcgac agagttcctc atcgacgggg
    20641 atacccttat cccaccattt gtagcaatgg atggtctggg agagaacgtt gccaagcaac
    20701 tggtgcgggc gcgtgaagag ggagaattcc tctctaaaac agaactacgc aagcgtggtg
    20761 gactctcatc aaccttggtt gaaaagatgg atgagatggg tattcttgga aatatgccag
    20821 aggataacca gttgagtttg tttgatgagt tgttttaaaa aattgcttaa taatctatta
    20881 aaagaggcta acgtatatcc aatagattta cattagcttt cttttttgtt aaaatagtct
    20941 atggaaagag ggtgagagta tgtcaaagat gagtataagc atccgtctgg atagtgaggt
    21001 taaggagcag gcccaacagg tgtttagtaa tctgggaatg gatatgacaa cagctattaa
    21061 tattttcctt cgtcaggcaa ttcaatatca gggattacct tttgatgtta gactagacga
    21121 aaatcggaag ttgctccaag cgttaacgga tttagaccaa aatcgtaata tgagccagtc
    21181 ttttgaatca gtctcagatt tgatggagga cttacgtgct taagattcgt tatcataaac
    21241 agtttaaaaa agattttaag ttggctatga agcgtggttt gaaggcagaa ttattagaag
    21301 aagttttgaa ttttctggtt caagaaaaag aacatcctgc cagatatcgt gatcattcat
    21361 tgacggcatc caagcatttt caaggagttc gtgaatgcca tacccagcca gattggcttt
    21421 tggtttataa agtagacaag tcggaattga ttttaaattt gctgaggaca ggcagtcaca
    21481 gtgatttatt ttaatctatt ttaagggggt tctcatgaaa ctaagaatat ttgcggaaga
    21541 taagccggct aagaaggtat ttgaatatca attagaactt gctgatcgta caattcttct
    21601 atcgacagca ctcttgtcag gtgctattgc tttagcagga atcttttctg ctttgaaaga
    21661 aaaataaaaa tagaaaagag aaaacagaat ggttttacca aattttaaag aaaatctaga
    21721 aaaatatgcg aaattgttgg ttgcgaacgg aattaacgtg caacctggtc acactttggc
    21781 tctctctatt gatgtggagc aacgtgaatt ggcacatcta atcgtgaaag aagcttatgc
    21841 cttgggtgcg catgaggtca tcgttcagtg gacagatgat gtgattaacc gtgagaaatt
    21901 cctccatgcc ccgatggagc gtttggacaa tgtgccagaa tacaagattg ctgagatgaa
    21961 ctatcttttg gagaacaagg ctagccgtct tggggttcgt tcatctgatc caggtgcctt
    22021 gaacggagtg gacgctgaca agctttcagc ttctgctaaa gctatgggac ttgccatgaa
    22081 gccaatgcga atcgcaactc aatctaacaa ggttagctgg actgtagcag ccgctgctgg
    22141 acttgagtgg gctaagaaag tcttcccaaa tgctgcgagc gacgaagaag cagttgattt
    22201 cctttgggat caaatcttca aaacttgtcg tgtctacgaa gcagatcctg ttaaggcttg
    22261 ggaggaacat gcagccattc tcaagagcaa ggccgatatg cttaataagg agcaattttc
    22321 agcccttcac tacacagcgc caggaacaga tttaacactt ggtttgccaa agaaccacgt
    22381 ttgggaatca gctggtgctg tcaatgcaca gggcgaagaa ttcttgccaa atatgccgac
    22441 agaagaggtc ttcacagcgc ctgacttccg tcgtgcagat ggttatgtca cttctacaaa
    22501 accgcttagc tacaacggaa atatcattga aggcattaag gtgaccttta aggatggaca
    22561 aatcgtagat atcactgctg agaagggtga tcaggttatg aaagaccttg tctttgaaaa
    22621 tgcgggtgcg cgtgccttgg gtgaatgtgc cttggtacca gatccaagtc caatttctca
    22681 gtcaggcatt accttcttta acaccctttt cgatgaaaat gcgtcaaacc acttggctat
    22741 cggtgcagcc tatgcgacta gcgttgttga tggagcggag atgagcgaag aggagcttga
    22801 agctgcaggg cttaaccgtt cagatgttca cgtagacttt atgattggtt ctaaccaaat
    22861 ggatatcgat ggtattcgtg aggatggaac gcgggtacct cttttccgta atgggaattg
    22921 ggcaaattaa ggagataata tgttaggaag tatgttcgtt ggtctcctag tgggattttt
    22981 agcaggtgct atgaccaatc gtggagagcg aatgggatgt tttggaaaaa tgtttctcgg
    23041 ttggatcgga gcctttctag gtcacttgct ctttggaact tgggggccag ttttatcagg
    23101 aacagctatt atcccagcga ttttaggagc catgattgtt ttagctattt tttggagacg
    23161 aggaagttaa tttcctaaat ctgatactca atgaaaatca aagagcaaac taggaaacta
    23221 gccgcaggtt gctcaaagca ctgctttgag gttgcagata gaactgacga agtcagctaa
    23281 aaacactgtt ttgaggttgc agatagaact gacgaagtca gtaacatata tacggcaagg
    23341 cgacgttgac gcggtttgaa gagatttttg aagagtatga aacgaaaagg aggttggtca
    23401 ttgtaccagc ctccttttga tatgatataa tagttctatg agattagata aatttttagt
    23461 tgcctgtgcg gtgggaagtc ggactgaggt caaaaacttg ctcaaggctg ggcgcgtgac
    23521 tgtaaatggt aaaaaagaaa aatcagctaa attgcagatt gatgaaaaaa tagatgagat
    23581 tcgctttgat gggcaagtgt tggagtatga agagtttgtc tactacatga tgaacaagcc
    23641 caaaggagtt atctcagcga ctgaggatcc caagcacaga accgttctgg acttgttgga
    23701 tgacttggcg cggagtaagg aagttttccc agtaggacgc ttggatattg acacgcatgg
    23761 tcttttgctc ttgaccaatg atggtcagct ggctcatgtt cttctttcgc ccaagcgtca
    23821 tgtggataag acttatctgg cacaagtcaa gggaatcatg acccaagaag atgtggagac
    23881 atttgctgag ggtattcctc tcaaagactt tacctgtcaa cccgctatac tggagcttgt
    23941 atccatagat acagaaaaga atcagagcca aatccgtgtg accattgcag aagggaagtt
    24001 tcatcagatc aagcgtatgg tgggctactg tggcaaggaa gtggtggact tgcaacgatt
    24061 gactatggga actttagtat tagatgagaa cctggaacga ggggaatggc gtcgcttgac
    24121 caaggaagaa ttagaaattc tccgcgctaa tattatttaa tttagtttaa tactcaatga
    24181 aaatcaaaga gcaaactagg aaactagccg caggctgtac ttgagtacgg caaggcgacg
    24241 ttgacgtggt ttgaagagat tttcgaagag tataaattcg aaaaaggtga gggagaatgt
    24301 ccttaccttt tatgtttttt agttgcttcc tttgtgaaaa gagttataat agactgtaga
    24361 ataaaaggag gaatctatga acgcgattca agaatcattt actgataaac tatttgccaa
    24421 ctatgaagca aatgtaaaat accaagcgat tgaaaatgct gccagccaca acggaatttt
    24481 tgcagctctg gaacgtcgcc aaagccatgt agacaacaca cctgttttct cattggattt
    24541 aaccaaggac aaggtcacta accagaaagc gtctggtcgt tgctggatgt ttgcggctct
    24601 caacaccttc cgccacaaac tcatctcgca atacaaattg gagaactttg agttgtcaca
    24661 agcccacact ttcttctggg ataagtatga gaaatcaaac tggttcttgg agcaagtcat
    24721 tgcgacttca gaccaagaat tgactagccg caaggttagc ttcttactcc aaacacctca
    24781 acaagatggt ggtcaatggg atatggtcgt ttccctcttt gaaaaatacg gtgtcgtgcc
    24841 taagtcagtt tatcctgagt ctgtttcatc tagcagcagt cgtgagctaa atgcgatcct
    24901 taataaattg cttcgtcaag atgctcaaat cttgcgtgat ttgcttgttt ctggtgcaga
    24961 tcaagtgact gttcaagcta agaaagaaga cctcttgcaa gaaatcttta actttcttgc
    25021 tatgtcatta ggacttccac cacgcaagtt tgactttgct tatcgcgata aagataacaa
    25081 ctacaaaagt gaaaaaggaa tcacaccaca agagttctac aagaaatatg tcaatcttcc
    25141 tttagaagac tacgtttctg ttatcaatgc tccaactgct gataaacctt acggaaaatc
    25201 ttacacagtt gagatgttgg ggaatgtggt tggtagccgt gcagttcgtt acatcaacgt
    25261 tccaatggag cgcttgaaag aattggcgat tgcccaaatg caagcaggtg agactgtttg
    25321 gtttggttct gatgtcggcc agctcagtaa ccgtaaggct ggaatccttg cgacagatgt
    25381 ttatgacttt gaatcaagca tggacattaa acttactcaa gacaaggctg gacgtttgga
    25441 ctatagcgaa agcttgatga cccacgccat ggtcttgaca ggtgttgact tggacgaaaa
    25501 tggtaaatca accaagtgga aggttgaaaa ctcatgggga gacaaggtcg gtacagatgg
    25561 ttactttgtt gcctcagacg cttggatgga cgaatacaca taccaaatcg ttgttcgtaa
    25621 ggaattgctg acagcagaag aacaagctgc ctatggagca gaaccaattg tacttgcacc
    25681 atgggatcca atgggagcct tggctaaata aaagcataga aaaaaggaat cagattttag
    25741 aacctggttc ctttttagtt gcttgattac atgatgtgaa gaacatgtgc cacaataccc
    25801 actgcgaaga gggcaaggat aatagtgatt ggagatactt ttttcttaag taagtacatg
    25861 caaagtaaag taaggagtag tcctgataat ccaggaatca acatatccaa gttttgttgg
    25921 aaagtagtaa ctttttcagg agtttgagac aatccttgtc ctacttgtgc gaatgcttct
    25981 tggatacctt tagacccttc tggcaattta tcccaatgga tataagcctt ttcatctagt
    26041 tgaactttag aaacatcgaa agcaaattta atatttaccc agcgttgaac aaggacagca
    26101 agaatgaaca tcccaaggat agaagctcct ttagtgatat cttgaaggat accaccagac
    26161 atatctttag tgatttctga tccagccttg tatccaatct cttgaacata ccacaagaat
    26221 gacatacgaa tcaagttcca tgcaacaaag aagaggagtg gccccaagat attgccagta
    26281 agggcaagtg aagcaccgag agatccaagg attgggcgta ctgtaaacca gaatactggg
    26341 tcaccgatac cagcaagagg tcccatcata ccgattttaa ccccttggat agcagcgtca
    26401 tcgatttcca caccgttagc acgttcttct tcaagcgcaa gagtaacccc catgactgga
    26461 gcagctacgt atggatgagt gttgaagaac tcaaggtgac gctcaagagc agcgatttga
    26521 tcttcttttt tagtatagag ttttttgata gctggaatga gtgtataagc ccagcccaag
    26581 ttttgcatcc gttcaaagtt ccaagaccct tgtaagaagg ttgaacgcca ccaaactttt
    26641 ttacgatctg atttagttaa ttgaagtttt tcagtcatga tgttttcagt cctttcttat
    26701 cttagtagtc ttctaggata tcgccgattg ggtcgttaga agttgcggct cctccgccac
    26761 catttccacc agttttagaa aggtgaaggt agataagagc gatagcaaca ccgatagcac
    26821 cgaatccgat tagagtaata tctgacacag cagcgagaac gaaaccaaga gcgaagaatg
    26881 gccatacttc acgagttgcc atcatgttga taaccatggc gtaaccaacg gcaacgacca
    26941 taccaccacc gatagccata ccatctttga gccagtctgg catggcacta aggatacttt
    27001 gtacagtttc agttggtacc ataaggagaa gagctgcagg aagcgcgata cgaagtcctt
    27061 ggaaaagtag cgcaatgaaa tgcgcacgct ccacagcgcc gaagtcacct tttttagcgg
    27121 cagcatctgc ggtatgaacc aaaccaactg aaattgtacg aacaatcatt gtcaagaaaa
    27181 gtccagctac ggcaagaggg atagcaaccg cttgggcaac accgatacca gtcttggtaa
    27241 agtcaccacc aagaaccatg ataatgacag cagcgacaga agcaagtgca gcatcaggag
    27301 cgatagcagc accgatattt gaccaaccaa gggcaatcat ttgaagcgat ccaccgagga
    27361 taatccctgc ttccaagtga cctgttacaa gcccaataag ggtacaggct acaagtggtt
    27421 ggtgaaattg gaactggtcg aggatgcctt caagacctgc aaagaaggct acaacgatta
    27481 ctaaaaccat agaaataata gacatgttta aaatcctttc ataaataatg gcttatttga
    27541 cattggcttt gttaatcaag tcaaacaaat cttttttaga atcatttggt actttacgga
    27601 catcaaattc aacacccaag tcacgcattt tttcaaatgt agcaacatct tctttgtcca
    27661 tagacaaaac ggtattgacc aatgttttac ctgttgagtg agccatagaa ccaacgttaa
    27721 gagtcttgat tggcacgccg ccttcgatgg cacgaagggc atcttgaggt gtttcaaaca
    27781 agataagggc atgtgtttct ccaaaacgtg ggtcttttga aatctcaatc agtttttgaa
    27841 ttggaaccac gttagccttg acattacctg gagctgcttg tttaatcaat tctttacgaa
    27901 ggtcgtcttt agccacgtta tctgaagcaa cgatgatacg atttgctttt gaatctggag
    27961 tccaagcagt tgcaacctga ccgtgaagta gacgtgtgtc aagacgggca agattgattt
    28021 tcaatttacc gtctccgata acagttcctt ctgggatagc agtttgggca actggagcag
    28081 ctgcagcgct tgcaacttct tcaactggat ttagctcttc tggaagagct ttgatgccat
    28141 ctttggcttc tttaatgata ttagcagcga ctttttctac acctgcagca gcgtccatga
    28201 ggcgctctgt gtaggcttga atcaacatcg gtaagttaag tcctgtgatg atggcaaact
    28261 tacgctcagg attttctccc atcacgcgac tagcttggtt aaatggagaa ccactccaaa
    28321 ggtcagccaa aactagaacc tcatcttctg cgtcaaatgc agcaacagcg ttattaaact
    28381 tagcgtatag atcatcagga ccttcatttg gcataaaggt cacaacttga accttttctt
    28441 gttcaccaaa gatcatagat cctgactgat gaataccagc agcaaattcg ccgtggctcg
    28501 caataatgat tccgatactc attattgtca ttcctccttt tttgttttag ttttcattta
    28561 agaaaacttt aagactgttt aagtataaac cgttttca
//
LOCUS       DAVKDZ010000021        28186 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000021 DAVKDZ010000000
VERSION     DAVKDZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28186
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.021"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            92..162
                     /locus_tag="VWZ30_000899"
     tRNA            92..162
                     /locus_tag="VWZ30_000899"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:124..126,aa:Cys,seq:gca)
     gene            complement(360..1691)
                     /locus_tag="VWZ30_000900"
     CDS             complement(360..1691)
                     /locus_tag="VWZ30_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU9334663.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(1982..2806)
                     /gene="endA"
                     /locus_tag="VWZ30_000901"
     CDS             complement(1982..2806)
                     /gene="endA"
                     /locus_tag="VWZ30_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEU9334664.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     APEAPSQELAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNREETGNGSTSWTPPGWHQVKNLKGTYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEDSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(2845..3033)
                     /locus_tag="VWZ30_000902"
     CDS             complement(2845..3033)
                     /locus_tag="VWZ30_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9334665.1"
                     /translation="MNKKSGYVIKRLLLVILVLILGLLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(3026..4309)
                     /gene="murA"
                     /locus_tag="VWZ30_000903"
     CDS             complement(3026..4309)
                     /gene="murA"
                     /locus_tag="VWZ30_000903"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9334666.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASERETVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(4333..4563)
                     /locus_tag="VWZ30_000904"
     CDS             complement(4333..4563)
                     /locus_tag="VWZ30_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEU9334667.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(4621..5658)
                     /locus_tag="VWZ30_000905"
     CDS             complement(4621..5658)
                     /locus_tag="VWZ30_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU9334668.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMVYAWLTPFTDIRSVQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPALRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGGAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(5642..6130)
                     /gene="coaD"
                     /locus_tag="VWZ30_000906"
     CDS             complement(5642..6130)
                     /gene="coaD"
                     /locus_tag="VWZ30_000906"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU9334669.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLCVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(6120..6659)
                     /gene="rsmD"
                     /locus_tag="VWZ30_000907"
     CDS             complement(6120..6659)
                     /gene="rsmD"
                     /locus_tag="VWZ30_000907"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU9334670.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(6724..7716)
                     /locus_tag="VWZ30_000908"
     CDS             complement(6724..7716)
                     /locus_tag="VWZ30_000908"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU9334671.1"
                     /translation="MKKSFIHQQEEISFVKNTLTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(7827..8510)
                     /locus_tag="VWZ30_000909"
     CDS             complement(7827..8510)
                     /locus_tag="VWZ30_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU9334672.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFFASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(8566..9306)
                     /locus_tag="VWZ30_000910"
     CDS             complement(8566..9306)
                     /locus_tag="VWZ30_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU9334673.1"
                     /translation="MTIITSKANSVVKNTKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFTLENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQREEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            9340..9618
                     /locus_tag="VWZ30_000911"
     CDS             9340..9618
                     /locus_tag="VWZ30_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU9334674.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYPDFKIAN"
     gene            9696..10622
                     /gene="yidC"
                     /locus_tag="VWZ30_000912"
     CDS             9696..10622
                     /gene="yidC"
                     /locus_tag="VWZ30_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9334675.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFASQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIINYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(10697..11491)
                     /gene="pflA"
                     /locus_tag="VWZ30_000913"
     CDS             complement(10697..11491)
                     /gene="pflA"
                     /locus_tag="VWZ30_000913"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEU9334676.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(11605..12867)
                     /locus_tag="VWZ30_000914"
     CDS             complement(11605..12867)
                     /locus_tag="VWZ30_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU9334677.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYTRELGATINLDAFEDIEHLERAAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTPAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKKTYRTYLGVDASAINLMRPAMYGAYHHITNLTHPDGPAEVVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     VRQIRRAERPKDYFATLYGFDFEEESEK"
     gene            complement(12934..13761)
                     /gene="purR"
                     /locus_tag="VWZ30_000915"
     CDS             complement(12934..13761)
                     /gene="purR"
                     /locus_tag="VWZ30_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEU9334678.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQVIDVEVGNIFDEDK"
     gene            complement(14028..14969)
                     /locus_tag="VWZ30_000916"
     CDS             complement(14028..14969)
                     /locus_tag="VWZ30_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEU9334679.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQVGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(14959..16215)
                     /locus_tag="VWZ30_000917"
     CDS             complement(14959..16215)
                     /locus_tag="VWZ30_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU9334680.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIKLHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQVSFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYEVGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(16217..16879)
                     /locus_tag="VWZ30_000918"
     CDS             complement(16217..16879)
                     /locus_tag="VWZ30_000918"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU9334681.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMRQIAIEDGQNLIAYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIGREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(16842..17498)
                     /locus_tag="VWZ30_000919"
     CDS             complement(16842..17498)
                     /locus_tag="VWZ30_000919"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU9334682.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVANPENHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            complement(17509..18387)
                     /gene="rsgA"
                     /locus_tag="VWZ30_000920"
     CDS             complement(17509..18387)
                     /gene="rsgA"
                     /locus_tag="VWZ30_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU9334683.1"
                     /translation="MQGQIIKALAGFYYIESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNNLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIIYISKMDLLEDRSELDFYQQTYGDIGYDFVTSKEGLLPLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     TAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGTIAPFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(18389..19261)
                     /gene="rsmA"
                     /locus_tag="VWZ30_000921"
     CDS             complement(18389..19261)
                     /gene="rsmA"
                     /locus_tag="VWZ30_000921"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU9334684.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGTLTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESDIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLT
                     EFASLAAALKGQGL"
     gene            complement(19392..19700)
                     /locus_tag="VWZ30_000922"
     CDS             complement(19392..19700)
                     /locus_tag="VWZ30_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334685.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLWVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            complement(19693..20334)
                     /locus_tag="VWZ30_000923"
     CDS             complement(19693..20334)
                     /locus_tag="VWZ30_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9334686.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKIIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVEALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVGLKDENRMIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(20336..22366)
                     /locus_tag="VWZ30_000924"
     CDS             complement(20336..22366)
                     /locus_tag="VWZ30_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9334687.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWNQFSDRYRLSFGW
                     SSVFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPDSLREQSAYYQGLFTDYL
                     QNFSSESVEVTSRKHYFPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLSGMTIYELHGKYLLAQGG
                     VLLLGLILSSVLTGDSLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(22635..23534)
                     /locus_tag="VWZ30_000925"
     CDS             complement(22635..23534)
                     /locus_tag="VWZ30_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9334688.1"
                     /translation="MLIGQKIKEIRIEKGVSRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFLFLWKQALREERDFSLAESFYQSSKTFAQ
                     LIGDEFLVKKLTEEWQEDVKKYL"
     gene            complement(23606..24166)
                     /gene="rnmV"
                     /locus_tag="VWZ30_000926"
     CDS             complement(23606..24166)
                     /gene="rnmV"
                     /locus_tag="VWZ30_000926"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEU9334689.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(24166..24939)
                     /locus_tag="VWZ30_000927"
     CDS             complement(24166..24939)
                     /locus_tag="VWZ30_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU9334690.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQIYATIGWHPTEAGTYTEEVEAYLLDKLKHSKIVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIAYLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(25244..26074)
                     /locus_tag="VWZ30_000928"
     CDS             complement(25244..26074)
                     /locus_tag="VWZ30_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9334691.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLETTPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKNKQDEIKGAPLSDKEKAELLARVEAETIGVQAIAMVTVPKRPVAPNAAPKT
                     TSAPQATAGTMQDVTYQSPAGKQLPNTGSVSSAALASLGLVVATSGFALLGRKTRRRK
                     "
     gene            complement(26358..26492)
                     /gene="rpmH"
                     /locus_tag="VWZ30_000929"
     CDS             complement(26358..26492)
                     /gene="rpmH"
                     /locus_tag="VWZ30_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU9334692.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(26637..27851)
                     /locus_tag="VWZ30_000930"
     CDS             complement(26637..27851)
                     /locus_tag="VWZ30_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9334693.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYFGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(28007..>28186)
                     /locus_tag="VWZ30_000931"
     CDS             complement(28007..>28186)
                     /locus_tag="VWZ30_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334694.1"
                     /translation="YPSFIGFLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLY
                     DSFLGLLFLGLSFGM"
BASE COUNT         8053 a         6605 c         5297 g         8231 t
ORIGIN      
        1 cttggcatca cattcgaatc acgctatggt acaaaaaact gaaaaaacta ttgactgaaa
       61 atcattttca aggtataata ataaacgtta aggcggtata gccaagtggt aaggcacggc
      121 tctgcaaaag cttgatcgtc ggttcaaatc cgtctaccgc cttctataac ttgatttatc
      181 aggtttcaaa tgaacagaaa gcccaatttg aagggctttt tttattttcc ctcgaataaa
      241 tacgtataac tttaaaaact tttggagcga gtttgtggca gagttctttc catggcataa
      301 ttcccttttg aaatcaattg aaaaaagctt cgtcacgcct ggtgacaaaa gcctctagtt
      361 tattctgttt cttcttctat ttcgacttct gtgatttgga cttttacctt ggtaacacgt
      421 ccattcttca ctttatcatt ggttaagata aactgtttgt tttggctgac caattcatag
      481 ctgagtttct cagtcgttgg aatggttccc actcctgtca aataataacc agcgatggta
      541 tcaacgtcat cactttccag ttcaacatca aagtaattat tgaaatcatt gagggtcata
      601 gttccttgaa caatgtaggt atcctcgcca atctgatgaa catctattgc tgctttgtcc
      661 gtttcatcat caatttcacc aacaatttcc tctaagaggt cttccaaagt caccaaacca
      721 gccataccac cgtattcatc cagcaagatt gccatttgtc tttgggtatt tcgcagttct
      781 tttagcaagt catccacaaa aatagtttca ggtacaaaaa gtggatcttg taaaattctc
      841 ttccaaacaa tattgtcaaa accgtccaca aagcctgcct taaggagact cttggtgtga
      901 atgattccaa ttacattgtc cttatcccca tcataaaccg ggatacgaga ataattttgt
      961 tttaaaatac tttggataat ggcttgacta tcatcctgaa tatccaccat aaaggcatcc
     1021 gttcgaggaa ccataacctc tcgtgccatc agttcatcga gcgaaaagac accttgtagc
     1081 atctcaatct catcagcatc caatgtttct tcactatttg tcaacatata ggcaatttca
     1141 tcacgggtca tcttttcatc cgcatcatcg aatgacatag gagtcaaatg gctcaagaaa
     1201 ttggtcgaag cagctaaaag ccaaacaaaa ggactgacta gttttccgat cccaatgata
     1261 atcggcgctg tacgaattgc caaggcatcc tttagattaa gagcgattct cttaggatat
     1321 aattccccaa aaacgatgga aatataggtc aaaaatgcca aggatagaaa agttgccacg
     1381 gcttgtgctg tttcgccatt cccaagccaa gaggcaatca cacgtcctag agtatcagtt
     1441 aaactcgccc ctgataagat tgtaatcagg gtgattccta cctgaatggt tgataaaaag
     1501 tggttaggat tttctagtac cttcagcagg cggatgtagc gtctgtctcc ttcttccgcc
     1561 ttttgttcaa ctcgggcacg attaagagaa accatggcca tttctgtggc tgagaaaaag
     1621 gcatttaata ccgtcaagat aaacaataaa acaaattgta gcaacaaatt ctgactactt
     1681 gggtcttcca tagttcttct cctaaaatag tatcttatcc tttattatat cacaaaattt
     1741 aatccaatac atattatttt tttcttagcg taacttccat ctctctccca aaactggaag
     1801 ttagtctaag acggatttcc actgatttca cgcgtttttc tcatactaaa agctaggttg
     1861 tgggaaactg gaagttagtt ttagaagtta ccattttttc ttagtaccta ttctagccct
     1921 gcttaaacta gtaaaaagaa ctcctacatc cataagtgac ataggagttt aggcttatct
     1981 tttactgagt tacagttact tctccagttc ggtaatccag ttgaagtccc ttttgaacat
     2041 tgggaactag aacattgaat tccaattctc catccgaaga cttggcttca atctgtgaag
     2101 ctgagggaac taaatcctcg tttgaagcgt agtaaagggt tacacggtaa cggacacgct
     2161 tgttttggtc caaggcttta cgcaccttgc tttcatagta gttttgacca gtcgaatcct
     2221 cggcttgtgc ctgatttgcc caggctgttt gaacagcaat gtttttagga ttgcttgtcg
     2281 aggcatcaaa accatccaag ccaccgatta aggcatagcc taacaagtga cctctatcga
     2341 ccgcatgggt ataagtcccc tttagattct tgacctgatg ccaacctgga ggagtccaag
     2401 aagttgaacc attcccagtt tcttcacgat tcttgtactg acgagtggcc ttagacaaaa
     2461 gggcattagc tacggttgga acagtttcct tgcccactgt ctttgtttta ttgtcagcgt
     2521 agggcttact tgaaaccttg gcatctagat ttgttttatt accattgacg ataaaagcac
     2581 ctgagccatt ccactccaga ctccccttta tttgactctt gactgcgtct gttaagacac
     2641 tttctgccaa ttcttgacta ggagcttcag gcgcttgttt tttctgacta agattggttt
     2701 tgggactatt aggtgccgac tgcatctgct tgatataata gctccctgta gacaaaagca
     2761 ataacactag cagtccgatt agtgtctgtc ttgttttttt gttcatattt ctccttatct
     2821 ttagaaaaag gctggttctc cagcctaatt tcctgtaaat ttatgaatca attcctgcca
     2881 ttttgccgga gacaggatag cccacggatc ttgacccttg cccaagattc catagcctac
     2941 cattaaaccg attcctaagg ctagaaggcc taaaatcagt accagaatga ctaaaagtaa
     3001 acgcttgatt acgtaacctg atttcttatt catcttcatc acttgcctca atccgctgaa
     3061 tcttagcacc tagctgcgcc aacttctcat ggaaaccgta gtaacctcta tccaagtgaa
     3121 ccaatttacc gaccacagtt tctccctgtg ctaccaaacc tgtcaaaatc aaagctgcac
     3181 tggcacgaag gtcagttgaa agaacttctg ctccctgcaa aggctgtcca ccaacaatac
     3241 gagctgtatc acggataatc tcagaatgca agcccatgcg gcgcatctct tctaggtgtt
     3301 ggaaacgatt ttcgaaaact gtctccacca tggttgattc gccttttgca actgtcatca
     3361 aggctgtaaa ttgagcctgc atatctgttg gaaatcctgg gtggggcaag gttttcacat
     3421 gaacagcttt tagattttct agttgagaac gaacacgaat tccttcgtct tcttcaatta
     3481 cttcaacacc catttcaagt aacttggcaa tcaagggacg gttgtgctcc cagacagcgt
     3541 ctcgaatcaa gacatcacca ccagtcatgg cagcagctac cataaaggtt cctgcttcga
     3601 tacgatcttg gactacattg tgagtcgtac catgaagttt ctcaacacca gtaatggtta
     3661 tagtctctgt accagcacct ttgaccttgg ctcccatttc attaaggaga atggctaagt
     3721 caacaatctc aggctcacgc gcagcattct caatcactgt caccccatca gccagagtcg
     3781 ctgccatcat caagttctgc gttgcaccaa cacttggaaa gtccatatag atatgagcgc
     3841 catgcaagcg ttctgccttg gcttctatgt aaccagctgt ctgactaatc ttaaccccca
     3901 tagcttccag acctttcaaa tgaagatcaa taggacggct accaatcgta caaccacctg
     3961 gcatggatac cttggcatga cccacacggg caaggattgg ccctaataca acgatggagg
     4021 cgcgcatctt gctgacatac ttgtaagggg cttcctcagt gatgtcgcca gtagcatcca
     4081 ccttgacaag atgagcttcc tcatcaaagt caaccttggc attcaaacca ccaactacct
     4141 gattcataat aaagacatcc gacaaaatcg gaacattctg caagacggtc tctctttcac
     4201 ttgctagaat agtcgctgcc aacaagggta agactgcatt ttttgctccc tcgatcgtca
     4261 cgcttcctac cagacgatta tcgccacctt gaaccacaat tttatccata ctcgtttcct
     4321 ttactcttga ttttataata atcctctaag cgcttcttgc cacaaatgat acaggctaat
     4381 aaagaaagaa ctcaggatgt agcctatgac aatgctaaaa aaggctacta ataaacgaac
     4441 ttttcttgta ttatcatctg tcacttttaa aaccttctcc catctaacaa gatcctttaa
     4501 aaggtaaaaa ctcacataaa taaagagcat atgactactt agagtgaata ataattgaac
     4561 catttgacta ttataccatc ttctctgttt gtgcgctagt ttggaaactt cactcaaaaa
     4621 ttagggattg tttttcaagt aatcaatcgc atcttgtagg gttttaacgg gcacgatttt
     4681 catatccgtc ttgattgttt tagccgcttc tagggctgtt tggtagttgt ttttcgcgtc
     4741 cggatgcgcc ttttgttctt cttcgctaac agggttatca ggagcaaaga aaatagcacc
     4801 acctgcccta gccgaagcta caaccttctt atcaatacct ccaatgtctc ccacattacc
     4861 atcgcggtca atggtacctg taccggcaac aatacgacca ttacgaagag ctgggtgagc
     4921 tatttgagta tagatagcta gactaaacat gagaccagca cttgggccgc caataccagc
     4981 tgttgaaaag ctaattggga cattgctgat tacctctgta cgatcaatca agccgattcc
     5041 aatcccattt tttccatttt ccaaggtgat aatctttcct tctgcagact tggtttgccc
     5101 atcctcttca taggtgacct tgacggagtc ccctaatttt tgagaactga cgtaatcaat
     5161 caagtctttg gaactatcaa aggtctgatc attgactgct gtgaccgtat cagagatatt
     5221 gagaatccct ttaaaggttg aattatccgt cacattcaag acataaaccc caaagtactt
     5281 gagttcgata tccttaccag ctgtttttag accttgatac ttggccatat tttgtgatgt
     5341 ttgcatgtag aattgattga ttcgcataaa ttctacatcg gaagaaccac ctgtggtctc
     5401 ctgaacacta cgaatatctg taaaaggcgt caaccaagca taaaccatat gagctaatgt
     5461 ggcatgttga acaccaaccg taacgaattg ataggcacca gcttccttat cttctgtgtc
     5521 atttacttta aggacttggc gaatatcttc cgaaccacct ggaacctcta tataataagg
     5581 caagggcact acaaatgcca agaaagtcac aatcaaggcc gcaatgacgt ataagggcca
     5641 tctaatcttt tttttcattt cttatttcct ccaaaatact ctcgggaaca tagcaggcaa
     5701 tatcctgacc aaacttcaaa agctctctaa cgcctgatga actgatatag agatgttcag
     5761 gtcgactatg taaataaata gtctctatat cagaagacag ctgatgattg tagtaatcaa
     5821 aactggcttc atattgcaaa tccgacgcat tcctcaaacc acgcactaga caagtagcac
     5881 ccaatctttt tgcaacatcg accaccaatt catcatgaga agccacgact tcaacatttt
     5941 ccaaatgtcc caaagccttt tctagccccc gtttacgatt ttcaagaggg agaaatcctt
     6001 gtttgtgggg attaaaaaaa atacccacac aaagcttatc aaaaagtctg ctcgcccgtt
     6061 caatgatatc cagatgccca tttgtcatcg gatcaaatga gcctgtgaat aagccaatct
     6121 tatctgacat agactgtcac cttactaatt ccataaatct tttccttcca gatacccaga
     6181 caggcaatct cttctggaag ttcaacggcc ttatccgtct cgcacacaac cataacatct
     6241 tcagaaaaaa gctctctctc agccattttt tcaatatctg ctacgatttg ttccttggca
     6301 tagggagggt ctaagaaaac gaggtcaaat tccccagata cctgttccaa tgccctctct
     6361 gcatccatct tgaggagttg aaattttcca acttccttgg tcatctggat attttcagcc
     6421 acgatggtct gagccttacg gtctcgctcc accaaaacag cactggacat gccacgcgat
     6481 actgcttcga tagataaacc accactacct gcataaaggt ccaagactcg tcccccttca
     6541 aagtagggac caatcatgtt aaaaatggct cccctaacct tatccgaagt aggtcttgtt
     6601 gtcttgcctt ctagtgtctt gaggggacgt cccccataga ttcctgatac gattttcata
     6661 ctgtttattg taccaaatta tagacaaaaa gagaaagaaa accgaacctt gcggttcgat
     6721 tctctacaaa atattttcgt aagtatcgcg gacttcttga ggccaaacac ttgtttgcac
     6781 ttctccgatg tgtctcttgc gaagtaggaa catggccata cgagattgtc caattcctcc
     6841 accgattgtc aatgggaata ggccattcaa caaagacttg tgccattcca attccaagcg
     6901 gtcttcatca cctgtaattt ccacctggcg tctaagagtt tcttcatcta cacgaattcc
     6961 catagaagac aactcaaagg ctccacctaa agactcattc cagacaagaa tatcaccatt
     7021 tagacccttg tagccattct cagactcgct tgtccagtca tcatagtctg gtgcacgtcc
     7081 atcgtgcggt ttaccatctg gcaactcgcc accgatacca atcaaaaaga cggctccaaa
     7141 ttctttacaa atcgcatttt cacgttcttt cggtgtcaag tctgggtagc gttctaccaa
     7201 ttcttctgta tggataaagg tgatttgttt tggcaagata gactcgatgt catagcgagc
     7261 ttcaacagct agctcagtca ggcgaatagc cttataaatc ttctcaactg tttcttttag
     7321 ataaacgatg ttacgcttac catttgggat aaccttctcc cagtcccact ggtcaacata
     7381 aacagagtgg gttgcatcca aggaatcctc atctggacga agggctttca tgtggacaaa
     7441 gagaccctct ccttcaccaa agccaaaacg agccaaggtg tggcgtttcc atttagcaag
     7501 tgagtgcacc acttcataag tagcatcagg gatttggaga accttgaccg atactggatt
     7561 ttccacacca gacaggttgt cctgcattcc gtcaccgacc ttactcaaga taggaccttg
     7621 aacttcgaca acttctagct tatctttcaa atactgggtc aaagtgtttt tgacaaagga
     7681 aatttcttct tgttgatgga taaaactttt cttcatagac aactcctcaa aaatttaata
     7741 aacgtattat acacctattt ccaagaaaag aaaagagaaa atttaaaaaa atcagtgtca
     7801 ctcaaaacac tgatttcata gaccttttag tcattgcgac caaagatacg tagaatactt
     7861 aggaaaagat tgataaaatc aagatagatg ctgagagcca ttgacaccac ccagcctgtc
     7921 gctacacgac cttgtgattg ttcataggcg aggcgaatct tttggttatc ccaagcaatc
     7981 aaccctgaaa aaaccaaaac catggctacg ctaatcatat aatcaaagaa gccactagca
     8041 aagaaaatat tgactaccat agcaatcagt aaaccaatga gagctgccat cattgctcga
     8101 ccaatcccac tcaagtcttt cttggtaaag ataccaactg ccgccatgac aaagaagaga
     8161 agggcgctcg atacaaaggc agataaaact gtacccggag tatagaaagc caccacaaaa
     8221 ctaagggtaa agccgtttaa aacggagtaa agtaaaaata ctggaagagc agctggacta
     8281 tttcttgaag ccatgctact ggcaacgaaa accaaagcca actccgcaaa agtagcaatg
     8341 gtcaaccaga gacgcccctg catcaaaaag taaaccaact gagactgaaa gaccgtcaac
     8401 ataaggcctg ataccaaagc agatagtccg attcccagac caacaaaggc ataaacctta
     8461 gcgtaaaatt gattgagacc tgcacggtca tgtataatag tgtgattcat ccatttctcc
     8521 ttttctgtga acatctttcc ttgattataa caaaaaaagt taaaattagc ttaaatggaa
     8581 gattaaaata ccggctgcaa ccgcaacatt caaactctcg gcctgcccct tcatgctaat
     8641 atggaccaac tggtctgcac tttcagccat aaggggacta attccttgac cctcatttcc
     8701 cataactagt acaaaatttt ctatagaagg cagttctctg taatcaacag aatctttaga
     8761 tagggttgtt gctagcactg ggatagctac ctttttagtt tcgtcaagaa gcgcttgact
     8821 agtcatccgg taaatgggca gatggaaatg actaccttgc atggaacgta gggttttgag
     8881 actgtagatg tctgccgact tatctgaaac aatcactcct gtaaaacctg ctgcatccgc
     8941 agttcgaatg atagttccta cattaccagg atcttgcaca tcttccaaaa acaagaactt
     9001 gccctgactc aaatcagctt gtcctacttc ttctctttga accacggcaa caattccctg
     9061 tggagtctga gaatccgcca aatctagcaa aatatcctct gacacccaga cagtttgcga
     9121 aaaagtagct aactgatctc gataattttc tagggtaaag attttctcaa tcgtcactcc
     9181 agcttgaaca gcttcttcaa acaagtgcca gccttcaatt aaataggcag acttgcggta
     9241 ttttttttgg tgtaatttct tggtattttt tacaacagaa ttggcttttg aggttataat
     9301 agtcatagaa atattataac acaatcaaag gggtttggta tgcaaaaggt tagaatgatt
     9361 gcccaaggta gggtgcaggg agtcggcttt cgttggggtg tttatagcct ggcccttgaa
     9421 atcggtggca tcacaggtcg ggtatggaat aacgacgatg gcacagtgga aatcttagcc
     9481 caagcagact catctgctat catggcaaaa tttatccaag aaatccgaaa aggaccgaca
     9541 cctttttcaa aagtcagtta cttagatgtc aaactgagca actttcctcc ctaccctgac
     9601 tttaaaatcg caaattaggt ctccagaact attgtatatt ttgtaaaaaa acagtagaat
     9661 agaaaggtat aatttttaaa gaaggaataa aaacagtgaa atctattaaa cgttttgcat
     9721 tctcggctat gggagtggct atgttgcttg tcttgactgg ctgtgtcaat gtcgataaaa
     9781 ccacaggtca gccaacagga tttatttgga atacgatcgg agcgcctatg gctgaagcca
     9841 tcaagtactt cgctactgat aaaggtctag gctttggtgt cgctatcatt atcgtaacca
     9901 ttatcgtacg cttgattatc ttaccacttg gtatctacca atcatggaag gcaacgcttc
     9961 actctgaaaa gatgaacgct ctcaagcatg tccttgagcc acaccaaaca cgtctcaaag
    10021 aagcaactac tcaagaagaa aaactcgaag cccaacaagc cctctttgcc gctcaaaaag
    10081 agcacggcat cagcatgttt ggtggtgtag gatgtttccc tatcctcctt caaatgcctt
    10141 tcttctctgc tatctacttt gcttcccaac atactgaagg ggttgctcaa gcaagctacc
    10201 taggcattcc tctaggttct ccaagtatga ttttggttgc ctgtgctggt gtcctttact
    10261 atcttcaatc gctcctttca cttcacggag tagaagatga aatgcaaaga gaacaaatca
    10321 agaaaatgat ttacatgagc ccactcatga tcgtcgtctt ctccctcttc tcaccagcta
    10381 gtgtcacact ttactgggtt gtcggtggtt tcatgatgat tctccaacag tttatcatca
    10441 actatatcgt tcgtccaaaa cttcgcaaaa aagtccgtga agaactagcc aagaacccac
    10501 caaaagcaag tgctttctct aaaccaagtg gacgaaaaga cgttacccct gaacaaccaa
    10561 ctgctatcac aagcaagaaa aaacacaaaa atcgcaacgc tggaaaacaa cgttcgagat
    10621 aagaagaaaa aggcttagct gatttgctaa gtctttttgc aaatcaaaag cccgatgttt
    10681 ccatcaggct tcttttctat ccatgtacac gtttcatata atcttgataa ctttcggtat
    10741 ccatgagttt tttagcgttc ttgacgcgat ctgctgttgg tggtttgact ccttcgaggg
    10801 aatatggaat tccaagttca cgccacttga actcacccat ggtgtgataa ggtagaattt
    10861 caaacttatc aacatttttg agggtcttga cgaacttacc aagttcaatc aagtcatcat
    10921 ctctgtctgt caatcctgga actagcacat ggcgaatcca gacaggtttt ccaatatctg
    10981 atagatactg ggcacaagcc aagatatttt tattggtttg gctagtgaca atcttgtgct
    11041 gttcttcgtt gatttccttg atatccaaaa gaaccaagtc agtgacagcc atgagtttgt
    11101 caaacttctc aaggtaacgt ggtttattac ggaaaggaag agcacaggtg tccaaggtac
    11161 agtggattcc ttgttcctta gccttggtga agagggcaat caggaaatca atctgcaaga
    11221 gagcttctcc tccactgact gtaatcccac ccttatttcc ccagaaacca cggtagcgca
    11281 aggcctctgt caagacatca tctaccgtcc gttcacgtga cttattggac tccatagccc
    11341 aagtgtctgg gttgtggcaa tactggcaac gcatgtgaca gccctgcaaa aaaacaataa
    11401 agcgaatacc aggcccatct actgacccaa agctttctgt cgaatgcacc attcctgtca
    11461 cttgtccata atcaattgtt tcttcagaca tatcgttacc ttccttgaaa acgttttata
    11521 gttttattat atcacgactt gaaggaaaaa caaggttttt gcctattttt tagaaagcaa
    11581 tctgtttttc aatttcattt tcaattactt ctcagattct tcttcaaaat caaagccata
    11641 caaggttgca aagtagtcct taggacgctc tgcacggcgg atttgacgga ctttgccttc
    11701 ttcagtatag aggatttccg cagaacgaag tttggcattg tactggtaac ccattgaaaa
    11761 tccatgggca cctgtatcat gaatgaccag caaatcaccg atttctgtat gaggcagttc
    11821 gcgattcact gcaaatttat cattgttttc acagagtgaa ccgaccacat ctaccacttc
    11881 agctggtcca tctggatgtg tcagattggt aatatgatgg taggctccat acatggctgg
    11941 acgcataagg ttgattgctg atgcatccac acctagatag gtacggtagg ttttcttctt
    12001 atgagtgact cttgtgacca aagctccgtg aggtgccagc ataaaacgac ccaactcagt
    12061 aaaaatctta acctgaccaa gacctgctgg cgtaaggacc tcttcataca ccttacgaac
    12121 tccctcacca atcaaggcaa tatcgttcgg ctcctggtct ggacgataat taacaccaat
    12181 accgccagaa agattgataa agtctaacga aatgcccaac ttttccttga tttcaacagc
    12241 cagttcaaag agctgacgag ccaactctgg ataatagaga tgggtcacgg tattggacgc
    12301 taggaaggag tgaagcccaa aagtcttggc tcctttttcc ttcaagatag caaaggcttc
    12361 aaagagctgg tccttggtca tgccaaactt agcctcccca ggattgtcca taatgtctgt
    12421 ccccagttca aaaacgcctc caggattata acgacaagag atgatttctg gaatgcctgc
    12481 tgctctctcc agatgttcaa tatcttcaaa ggcatccaag ttaatggtcg cacccaattc
    12541 acgtgtatag gcgtattcct tgtctggcgt gttgttggaa gagaacataa tctcagaacc
    12601 cagaaagtcc agtttatggc tcatcaaaag ctctacataa ctagagcagt ccacaccaca
    12661 accttcttct tggagaattt tcaaaatagc tggagttgga gtagccttaa ctgcaaaata
    12721 ttccttaaag cccttgttcc acgaaaaggc ttggttgacg gctcttgcct tctcacgaat
    12781 ccccttctca tcatacaagt gaaagggagt cgggaactcg gcaacaatcg cttctaactc
    12841 ttctctatta ataaatggtg ttttcataag cttccttcta ttctgtttgc aaagaaaggc
    12901 tgggacaatc gttccaacct ttagttctat ctcttattta tcttcgtcaa agatattgcc
    12961 aacctcaaca tcgatgactt ggttcttgac atcaatattg gttaccttca agagtgactt
    13021 gtagtcaaac tgcttttcac gttcttcttg ggcattgtcc gcaaagaccg ctacacctgc
    13081 cagttctgag tcgaactcgc gcaagagact aatcatcccg ttgacagttc cgccaccttt
    13141 caagaagtca tccacaatca agacacggct gcctgccttg agactgcgtt ttgaaaggaa
    13201 cattttctcg atacggtcac cacttgaacc tgaaacatag ttgacgctaa cagttgaacc
    13261 ttcggtaatt ttcaggtcac ggcgcacaat gacaaaagag acattgagga cattggcaac
    13321 tgcatttgca agtggcacac ccttagttgc tacggtcata accgcgtcaa ttttttggtc
    13381 cataaagctt ttggcaataa tacgaccaat atttttcaag atggctggtg tgctaagcaa
    13441 atcagacaga tagatataac cacctggcaa gatacggtca ctttctgaca acttggtacg
    13501 caagtcttca accatttcct tagcatcctg actcgaaata gacggtgtga agatgacacc
    13561 tccgccagcc ccagtcactg tctggatatg accgatttca atttcctcaa aggcgcgttt
    13621 gataatgacg atatcttctg agatggatga tttagcagac tcatactttt cagcaaaagt
    13681 attgagacta gttagtttat aaggattatt aatcaaatag ttggaaatga caaccatccg
    13741 atcacttctt cttaatttca tttctatttc ctattccatt tttattcaaa aaatcaaaaa
    13801 gcaaactaga aagctggtcg ctggtggttc aaaacactgc tttgagattg tcgatagaac
    13861 tgacaaaccc tgtaatatac ctgcatatat acatacgaca aggcgatact accctagttt
    13921 gaagagattt tcgaagagta ttcatttttg tcttttactt attataccat attcacataa
    13981 aaaaacgaac attcttatcc taaaaaatgc tcatttttct taaattatca atctaaatct
    14041 ggtttataga aggaacgatt atccatagcg aagattttat tggtcatctc tcctttatcc
    14101 accaaagcca gagctgttga catcatcatc atgcttgcat ccagattgtc aatcatatgg
    14161 ataatctctg cctccataat acgtggacgg actggacttc catattcaag caagccgtgg
    14221 tgactgagga tgacatgacg aagcaaaacg acttcttcct tggtatcatc gatgccgagt
    14281 tccataactg tcttggtaat ttcgctatca atgagagcga tatgtccaag aagattacct
    14341 cgcactgtgt actctgtctg gtctggcccc gtcaactcga tgaccttagc caagtcatgc
    14401 aacataatcc ccgcatagag caggctctta ttgagctgag gataaacttc gctaatagcg
    14461 tctgccaaac gcaccatggt cgccgtatga taggccaagc ccgtttcaaa ggcatggtgg
    14521 ttggtcttgg cagctggata ggagtagaat tccttatcat acttggtgta gagatttcgg
    14581 acaatccgtt gccagacagg attttcaatt ttgaaaatca tttgcgacat gtagtcacga
    14641 atttccttga catcaactgg tgacttgacc ttgaaatcgg ctgggtcatt gggttcacca
    14701 acttgaggca ggcggagagt aatttgattg acttgagggg tattgttata aacttctcgg
    14761 cgtcctttca tgtggacaac cttacctgcg gtaaaggcct caatgttatg aggttgggca
    14821 tcccagagct tcccatcaat ctcgccacta tcatcttgga aggtaaaggc taggtagttt
    14881 ttcccagctc gagtttgcct caggtcagct gatttgatta ggtaaaagcc ttcaaataac
    14941 tcatcttttt tcatgtgact aatcttcata ttcttcctca ttttcttgaa aatggagtag
    15001 atcaagcgca ggctcacctt ctgacaactc aatgtgacgg agcgtccgct cgatagctat
    15061 ggtacgacgg tttaataatt catcaatatt gccagaggca tgttggagat gtttttgcgc
    15121 cttgaccaga ataccaccaa acttgccaaa ctcggtcttg acactggcaa gagtcttgct
    15181 gatatggtcg gcactctttt ggatattaag ggtcttgaaa ccaactgata gggagttaag
    15241 aagggctgat agggtacttg gtcctgcaac aataatctgt tcttcccgtc tcaaatcatc
    15301 aaagaagacc ggattgcgga cgatttctga gtagagacct tctgtcggaa caaacaaaac
    15361 tccaaaattg gtcgtccgag gtggtgctat gtacttgttc ctaatatccc tagcaaaacg
    15421 cttgacactt gctaggagtg acttacgaca gcgttcgatt tcatccttgt cacctacttc
    15481 ataggcttct tctaagcggt aataatctgc cagtggaaac ttagagtcaa ttggtaggta
    15541 gacgtattct tggtcgcctt gtccgggtaa cttgatggca tactccactc gttcactaga
    15601 gttttcaacc gttgcgtatt ctcgttcgta ctgggcaggt gtcatgatgt cttcaataat
    15661 ttgccccagt tgcaattctc ccagaatccc tcgcgtcttg gttccagaga gaaccttgtt
    15721 aagagctccg acatcacggg caactgtctg catttctcca aggccacgat tgacagactc
    15781 cagttgttta gaaactgtct caaaggaaac ctgtaagcgt gtctgcaagg tcttttctag
    15841 tttttcctcg accgtctggc gcatttgttc caaacgttgc tcatttgatt cctgcaaggc
    15901 ttggagacgt tggtctgtct tgtctctagt ttggaggaga ttatctgtca tttcttgacg
    15961 gacttgggtc agaccttgat gcaatttaat ccgcacttct tgcaaacggt cgctgacagc
    16021 cacttccaaa tctttttggt ctaactggct ggcttgtctg gcttggtcaa agcggtaatc
    16081 caactggtct gacaaatgat ctgcctgatc ctccaagctc ttacttaagt gtttctcctg
    16141 cctatcctgc ctttgccaaa tcagaaagag accagctagg ttggcaatta ataatagaat
    16201 aagtaaactt tccatcctac ctcctgtcct tgctatgcag tacgaccaca taaccatctg
    16261 ggcaagttac cgacacttcc ctacctatat attcgttaga agcgtacact tttttaaaga
    16321 aaaaattttc ctctgtcaac tcatacttgg ctccaagaat agtcagctgg ctatcccgaa
    16381 ctggcataaa ggctagatag tcgtagtctg aacgaggttc tagctgactg gtcccttctg
    16441 gacaataggc aatcaagttt tgcccatcct caatcgctat ctggcgcata tagggtgcca
    16501 acttaggatt gctaggtaga aagacattgg ccaacatatg gtcaatacgg ccacccaagg
    16561 caccgaaaat agtgacctga gcctgaggat tttgttcaaa gatggttaag agagccaatt
    16621 ccagatctgt atcatccttt tctggtcgtg cttggacaaa atactgggca cgtttttgaa
    16681 tcacctgtcg ctcttcttcc gtcacagaat caaaatctcc gactgctaga gcaagaggta
    16741 agtcttcttc caatacccag agcgagcctc gatccacccc aacaaaagca tcaaaatctg
    16801 tccgataatg accgcggttt ccgcctgcaa aaactgcaac cctagtccag ttgttttctg
    16861 agagtttgta ctcgctcatt gacttctccc ttaaagacat aggaacctgc tacaaaaaca
    16921 gtcgcaccag cttctttggc ttgagcaata gtttggtcat caatcccacc atccacttcg
    16981 atttcaaagt tcaaaccttt ttcctcacga agagcaacca actcacgaac cttatccatg
    17041 gtttctggca gaaaggcttg cccaccaaaa cctggattaa ccgtcatgac taaaacttgg
    17101 tcaactagat gaaggacgtg cttgatggct tcaactggtg tgccaggatt gataacgact
    17161 gaaggcttaa ctccgagtga acgaattttt tggagggcgc catgaatatg aggtgttgct
    17221 tctacatgga tactgatgat gtctgcacct gcacgcgcaa agtcttccag atgattctct
    17281 ggattagcta ccatcaagtg gcagtcaaag accatcttgc tatgaggtcg aagactctcg
    17341 accacacctg caccaaaact gatttgcggt acaaaatgac tgtccatgat atcgatatgg
    17401 gcatattctg ccccagttgc ttctaggcgt ttgatttcac gttcaaagtt ggcataatct
    17461 gctgctagaa ttgacggagc aatcttgtat tgagacatag gtttctcctt attttggaat
    17521 ttttttgctg acttttttat aggtttctct gcgattttca atttcgctga ggaattgcag
    17581 gtagttgtca aaacggaagg gcgcaatggt accttcttca actgctggct tgacagcaca
    17641 ggacggctca tgggtatggg tacaagtacg gaacttacag tctcgactga cagtagcaat
    17701 ctctggaaag gcctgattga ggtcttcagc cgttgacact tcataatcca gtgatgaaaa
    17761 gcctggtgtg tctgcgattt tacccccatt gaggttgtaa aaactaactg ctcgagtggt
    17821 atggcgcccg cgacccagac tgtctgagat ttctcccgtt tcaagattaa ggtctggtgc
    17881 gattttattg agaagggttg attttccaac acctgtctgc cccataaaga ctgtaacctt
    17941 gcctgttaac aaaggaagga gtccctcttt actggtcaca aagtcatagc caatgtcacc
    18001 ataggtctgc tggtaaaaat ccaattctga ccgatcttcc agcaaatcca ttttagaaat
    18061 ataaatgata ggatggatgc ccttatgctc caaaagaacc aagaaacgat ccagcaaatt
    18121 gctgttaaaa tcaggttcct tgacagacat gatcaccaca gcttggtcaa tattgacaat
    18181 aggcggacgg accagattgt ttttccgttc atgaatcttg aggatatagc cttctgaatt
    18241 ttcctcggca gagaaatcta cccagtcccc aacgtaagga gtatggcctt ttttacggaa
    18301 attcccacgc gcgcgtgttt gatagacctg gccatcactc tctatatagt agaagcctgc
    18361 caaggcctta ataatttgtc cctgcatctt agagcccttg ccctttaagc gcggctgcta
    18421 gactggcaaa ttctgtcaag ctgagtgctt ctccacgcac acttggtgac aagcctgctt
    18481 ggtccaaggc cttagtcagc ttgtccttga cttcttcagt cttgccaaag taacctgtca
    18541 ggttattcca taaggtcttg cggcgatgag tgaaactagc cttggaaacc ttaaagaaga
    18601 agttctcgtc ttccactgct acagctggct ctggacgacg caccattttc aagatggctg
    18661 aatccacatt tggcgctggc acaaagaccg tacgaggcac gataaaggca accttggctg
    18721 tcatgtaata ctgcacggcg atagacaagc taccgtaagc cttggtgtta ggctgggctg
    18781 aaatgcggtc cgctacttct ttctgcatca tgaccacaaa ctcacaaaaa ggaatgtcac
    18841 tctcaatcaa gtgcatgaga ataggcgtcg tgatgtagta aggcaaatta gccactacct
    18901 tgattggcag gtcaggattt ttaaaattct ggatatgttg cgccaaatca accttgagaa
    18961 tatcttcgtt aactacggtc acattatcaa aatcacgcag ggtatctgcc aaaattggca
    19021 ccaaacggtg gtcaatctca aaagccatga cttgggctgc acgctcagcc aaaaattctg
    19081 tcaaggtacc aatacctggc ccgatttcga tgacattgac ctgatcatca atttcagccg
    19141 tatccacaat tttttgaagg atattggtat ccgtcaaaaa attttgccca aaggactttt
    19201 taaaggtaaa accgtgacgc tccagcactg ccttggtcac gctataatct gcaattctca
    19261 tctattctct tttctattat ctgttactac tattgtaaca cattcccctt gtattttggg
    19321 aatgaaaatc ataccaaaaa actatctttc ctcatgaaga aagatagtct tggaaatctt
    19381 taaaagtggc cttagcagtt ctctcgaaac atgcgttcaa aaaaatcagc ttcttgataa
    19441 cttcgatttt cgggtagggt caaatccaca tcctcctcca catcatccac tgtcagttca
    19501 accgtctgag gtaaattttt caaacgataa tgcttttgcc tttgcccttc aatactagca
    19561 aaatcaagca aatagccatg cactcggatt tgaacccaaa ggagatgatt ggtcatattt
    19621 tccatgatga ggatatcttc taaaccacct ctggcactag gataatcaga taccctgcga
    19681 atacggattt tttcacacat gagcaagttt cctcatatca atgatttcat cagccttatt
    19741 ccagactagg ggattatggg tcgcaatgat gatcattcga ttttcatctt tcaagcccac
    19801 caagagattc ataacctcct ctgaattttc aggatcaaga gctgctgttg gttcatctgc
    19861 caaaatcaag ggtggatttt tcaaaatagt cttagcaagg gcaactcgtt gggcctctcc
    19921 cccagataaa gtataaattt tttgttctag atccaaatac cctagattaa ctttttctaa
    19981 agcctccact tgcctttcca aacgttctac ttttgaaatc ttttgaccaa caaagcccaa
    20041 atccaaattt tctttgattg attggttttc taaaaggcca aaattttgaa agagatagcc
    20101 catctgatct cgaaaatact cacgagtggg aatgattttt aaatctttcc cctgatagag
    20161 aacccttcct ccatctatct tctctagctt tcccaagata ttcagcagcg tcgtctttcc
    20221 gcttccactc ttcccgatta aggcatagac cttgcccttc tccagtttga gattcaaacc
    20281 agagaaaatc gctcggtcat taaatttctt ttccaatcct tgaatatcaa tcatcttatt
    20341 ttcctttcaa tactgccatg ctaccagctt cttccttttt atcctgcctt actaaaatca
    20401 agagggcgtt aagagtaaat aaagctacaa ctaaagcgct gatcaagcta tctcctgtca
    20461 aaacactaga tagaattagg ccaagcaaga gaactcctcc ttgcgccagt aagtacttgc
    20521 catgaagctc ataaattgtc ataccagaaa gacgtttaat cataagctcc cgtctaaact
    20581 gctcaaaata gagaagattc atggaatcaa ataacaagat agccgtagac agggtcaaaa
    20641 tcgtcccaat aaggagagag taaaactcca cccgtttgtc atttagtacc ttggcaaaaa
    20701 atagctggct ttttaccaag taagaaacct tgtgatacag accttgctct tttagagctg
    20761 tgatgctgtc tccatatcga tctagtttca aggcactatt agccgtagtt ccccacaaca
    20821 tccctgcaac aggtcttgtt ccagtcgctt gcggcgttag aactacaata atcggatctt
    20881 ttaggtactg gcgtgttgtc ttgtacccat cattatagag gaaacgttcc tgtcctgttt
    20941 ctgtaaaagc tagccttacc tgtgggaagt agtgtttccg actcgtcact tctacacttt
    21001 cagatgaaaa gttttgcagg taatctgtaa acaatccttg gtagtaggca gactgctctc
    21061 gcaagctatc aggcaagatc agcccaaact ctccctcaga caagttttgc atcttgtcca
    21121 taaactctga agaaacggta atcttttctt ctatcagata gcgtggtgag acatagataa
    21181 cattccctga cggtgtgtag tcactgagac gattgccatc taggtccact tctgctccat
    21241 ctgagaaatt gtcaacattg ctcataatat aaaaagagtc tgtattggct aaccgttctt
    21301 cagtaaatgt ctgccactca cgattatcct tacgcgttcc ttcttcatcg gcaaatacac
    21361 tagaccaacc aaaggataga cggtaacggt ctgagaactg gttccattta ttgctagctc
    21421 tctccatttc ctgcatttca cggtagtggg gtaggagagc tgtcgcactc gatccgacca
    21481 ccaatacagc taagagttgc cccaccatca tcaatgtcat catacgtttg agagggagtt
    21541 tccctttcaa tagatccacc agactatttt cctgtaaacc aagtagatag acgacactta
    21601 gtaaggtaga aatccctgcc aaggtcaatc cataaagtag aagagcaatg atgaccagtt
    21661 gcaccgttgc cataaacaag gcaccttgat accagagaat ccccaatccc aaaagactgg
    21721 ataccagcac tgagcagata agctgtctca catcttctaa cactggtctg agagcaactc
    21781 caaacaagct ctcaccagct attaagcgaa tccctgcctg acgaagggat ttgatccgat
    21841 aaataagggt cagactcata aaggtcagca gaaaaatagc taaactcagt agcaccatag
    21901 gggtggccgc caatagtaag actatcgaaa atggatcttc tccattcgaa acaaagcctt
    21961 gataaccaag ctctttcaag gtggtctgta agctcactcc atccaaactt cctgatacaa
    22021 tcaagtaaga ccctaataaa tcactcgttt ctgcactctc cttggaggaa atggtcagac
    22081 cttctggaag ctttccctca ccataagtgg cataggtaaa gcgtgtttct ccagcttcat
    22141 ttggctcaac aatccgacgg gcgatgagac tattatgctc taccgccaag tgactcaaag
    22201 cctctgtcac ctcttcgcga ctagctactg gttgatttat catcattcct gtcacatcaa
    22261 cagaaggata gttaccaaat aaaatctctt ctcgatggtc aacggaagtt atcaccatat
    22321 aaagcgatac caataccatt gaaatcaaaa taaataaacg tttcatcatt tcctccaaaa
    22381 agttacttgg tcacttagtg agaaggtgac cacagtcact gccatgttaa gaagtttatt
    22441 aagttttttc tactagcaac atcttcaaat atattatatc cgttttttca agaactatca
    22501 agggcatcaa tgtccttaaa atctaaccat ttcgtgtcat ttttagctta tatctaaaaa
    22561 aacagcccta aagacgaact ttagaactgt tttgatgcga gaacaaggac atttttgtca
    22621 ctgattcact atgtttataa atattttttg acatcctctt gccattcctc tgtcaatttc
    22681 tttactagaa attcatctcc aattagctgc gcaaatgttt tagaagactg ataaaatgat
    22741 tcagctaaac taaaatctct ttcttctctt aatgcttgct tccacaaaaa cagaaataca
    22801 agaggtttct tttgataatc ttgagtttta tccataattt cttgtaaaca cgatagataa
    22861 tcattaaaac aatcagttac tcctaccttt tcaagacaac acattcctga aataagagta
    22921 tctctaatta taaagaagtt ttctaattta gttacctttt tctgttccaa aagaaagagc
    22981 attagctttt cgatttgatt tataaatttg gcaagatctt ttctaatgag catttgataa
    23041 aaaaacaaac gaatcaacaa catatcattt gttgaataaa cttctttttc tatgatctga
    23101 ggcatatgtt cctcaagtat taactctgca tattcaggcc ttctagttct agaagtatcc
    23161 aaacacgcct gcaatacttc acacgctaat tgttcttctt taggaagatt atcaaagtat
    23221 gttttataaa tctcttccaa acacgcttcc ttcttatcgt actcctcttc tttaccatag
    23281 attggttcac gtaaaatctg gtatttcaat tctaaataag cagaaggaag agctgaaaaa
    23341 tccggcataa ggctataaac tggaactcct agccggcgag caatatagtc taacttgggc
    23401 aaactcggtt gcgaagctcc actttcaatt cgcgacagtt gacgaactgt cagttcttgc
    23461 tcatctccac aaaaatctgg acgactaact cctttttcta tccgaatctc tttaattttt
    23521 tgaccaatta acatcagacg cctcctcttc tacctgcttc gaaaaatact gtgaaactta
    23581 aaaaattgta acggctacat ctttcctact cataagattt catagcttct tccacttctg
    23641 ccaaagtaac cccaaacaac tctaggcgtt tgaggagttg cttgccgtta gaatagccga
    23701 ttcggagagt ctctccgaga tattctctac gcttacggct gtctgcccct gctagaaaac
    23761 caaggcgaat caaatcgcta cgactaatgt caaactgact ctcatgttca aattgttctg
    23821 tcacctgagc tagagccgtt ttcaggtctt catagctggc atgctcaatt cccagagaac
    23881 gccccttggt cttggacttg ggaacagctt catctcgctt gagaaaggca tgctgaactg
    23941 ttggaatggc cgtcataatc atgcgccgaa tccgctcccc attaaaatct gggtctgtaa
    24001 agacaatgac tccatgacgt tggtgtagac gctgaattcg ctctatatcc tgatcattga
    24061 tggcagaacc tcgtgtctca taggtctcca catcgaaata acgcttgaga ttgaccgtat
    24121 catcgcgacc ttcaaccacg ataacttgag aaattttctc tttcattact tgctgtccaa
    24181 tccaaaaatt cgttctgcat ttgcagtcgt tgctaccgcc agctcttctg tcgtcatacc
    24241 acgcaagtaa gcgataaagt cgaccacata gcgagtatag gctgttttat tttcacgacc
    24301 acgcttgggt acaggtgcta agtaaggcgc atctgtttcc accaacatct tgtccaaagg
    24361 taactcttta gctgcttctt ggaggtcagt tgccttcttg aaagtcacca ctcctgagaa
    24421 ggaaatggtc ataccaagat ccacaaactt ctctgcccac tcaagcgtcc ctgaaaatga
    24481 atgcatgata ccaccacgag gaccaacgcc ctcactcttg ataatctcat aggtatcttc
    24541 cagcgcatca cgggtatgga caacaaaagg caaatccaag tccttagata gctgaatctg
    24601 acggcgaaaa acctgctcct gcacctcttt gggcgctgtc atccaatggt agtctaagcc
    24661 aatttcacct aaagccacaa tcttggaatg ttttaactta tccaacaagt aagcctcaac
    24721 ttcctctgta taagtaccag cttctgtagg atgccaacca atagtcgcat agatctgctc
    24781 atactcatct accaactcca aggcatgctc aatcgtcggt ttatcaaaac caacaatatt
    24841 catctgtgtc acacccatct cagcagccaa ggcaatttct tctgcctcac gacctgcaaa
    24901 ttcttctaca ttcaagtgtg tatgtgtatc aaaaatcatc tcttctaacc tcgttttcta
    24961 tcttctatta taccaaaaaa gaggaggggc acctaatttt tcggtttccc ctcctctctt
    25021 caataaagag ctattctgct atcttttcta tccgatattg cccatctcct attccacagt
    25081 tagagacaga agagattggc tacattgata gaatattcct attcagctaa agtatatgat
    25141 tatcttgggt tctcactttt ctcacccatc actaggattg atgacttctc aaaatccccc
    25201 ttataattta actttaagta gagaatagaa ttttctagct gttctatttt ctacgtctag
    25261 tctttcttcc tagcaaagca aaaccacttg ttgccaccac tagaccaaga ctagcaagtg
    25321 ctgcacttga tactgaacct gtgttaggta attgtttgcc agcaggtgac tggtaggtaa
    25381 catcttgcat tgttcctgca gttgcttgcg gtgcacttgt tgtcttagga gcagcattag
    25441 gagccactgg tctcttagga actgtaacca tggcaatggc ttgcactcca atcgtttctg
    25501 cttccactct tgctaaaagt tctgcttttt ctttatcaga aagcggtgcg cctttgattt
    25561 catcctgctt gttcttggct gctttttcaa tggcattttt agctgattct ttttcagtag
    25621 ctaattgctt agaagcttgt gattgagctg agttttgtga acctgtgtga gctgatccac
    25681 ccgcctggct accaccgcct tcattcgcat tagcatctga gcttccagta ttgcctccct
    25741 tatctccacc tggtgtagtt tctagcttat actctggtgt agcttcttga gttacagatt
    25801 cacctttcgc agattgttga actgtatctt ttcctagaat cgtcactact gaaccatctg
    25861 ggaatgtgat tgttgctgta ccatctccag ctacattgat tgtagctccg tctaatgctg
    25921 aaccatttgc ttgtaaaata gcaaccttaa ctttttcttc atctgttaaa tgagttatat
    25981 cttttacatc tactctttga gcaggtgctt taacagttga tttttcagca gtcggtttag
    26041 tactatcttg tgcttttgga tcttgtgcta ccaattgatt tcctggaatt gtaatagctg
    26101 aaccatcttt aaagacgatt gtcgctgtgc catctccagc tacttcaact ttatagtaaa
    26161 ataaaataat aacaggacaa atcgatcagg acagtcaaat cgatttctaa caatgtttta
    26221 gaaacagagg tgtactattc tagtttcaat ctactataac atcgcttccg aatccatttg
    26281 catctactga acttttttac aaaaataaaa atcagtagac tcgagttcct actgattttt
    26341 gatagaattc gtttggatta tgcagccaaa actttgcgtc ctttacgacg acgagctgcc
    26401 aatacgcgac gaccattttt agttgacata cggttacgga atccgtgttt gcgcgcacga
    26461 cgaagtttac ttggttgata agtacgtttc acgatgaata cctcctcata gattttgtat
    26521 tcgtttagcc ggctataaag tgatcagtta ctaaacatac tttactattc tatctgattc
    26581 tttcactttt gtcaatagct ctaggcaaat gtttccagct ttttcgaatg caagccctat
    26641 ctacgatact gtttcaagaa acgagtcatc ttttcttgga tttgggctaa ctcatcaaca
    26701 cgaggaaggt aaacgatacg gaagtggtct ggttcctgcc agttaaagcc tcgaccatga
    26761 accaagagaa ccttttcctg cttcaagaaa tcaaggacaa actgctcatc atcatcgata
    26821 cggtacatat tgcggtcgat ttttgggaag atatagagcc ccgccttggg tttaacggca
    26881 gacaaacctg gaatatcttg aatggcatta tagatgaaat ttctttgctc gtagattcgt
    26941 ccaccaggaa gaagcaattc atcgactgat tggtgacccc ccaaggaagt ttgtacgact
    27001 tgttgggcca aaacgttaga gcaaaggcgc atattggaca gcatattgag cccttcgata
    27061 tagcccttaa catgagtctt agggccagac aagaccatcc aacccacacg gaaacctgct
    27121 atgcggtggg attttgacag accattcatg ctgacacaga agacatctgg tgccaagctc
    27181 gccacaggcg tatgcacatg tccgtccatt accatgcggt catagatttc atccgcaaag
    27241 ataatcaaat cgttttgacg ggcaatctca ataatctcca gcaagagttc cttaggataa
    27301 agggctccag ttgggttatt tggattgata aggacgattg ccttggtatt ggaagtgatt
    27361 tttgacttga tatcgtcaat atctgggtac cattcaacag cttcatcaca gatatagtga
    27421 acggcatttc ctccagctag gctgacagcc gccgtccata gaggatagtc tggcattggc
    27481 actaagacct catccccatt gtccaaaagc ccctgcatgg acatgacaat caactcactg
    27541 acaccatttc caaagtaaat atcatcaata tctacgtttg ggaatttctt cagctggcaa
    27601 tactgcatga tggccttacg ggctgagaaa atccctttgg agtcagaata gccttcacta
    27661 tcacgcgcat tcataatcaa gtcatgaatg acctcgtctg gcgctgtaaa gccaaattct
    27721 gctggatttc ctgtattcag acgtaaaatc ttttctccat tcgctcgcat ccgcatggct
    27781 tcttccaata caggaccacg gatatcataa gcaacatgct ctaacttact agacttgtta
    27841 tattctttca tcttgtttcc tcaattcatc aggatagtaa cattatacca ctagaaagag
    27901 aatgcgacaa gtttactgaa atatgttact agtttcgtat agaagaaaaa ccttgctaaa
    27961 ggcgaggtta agaacgtaga gatagcaggt attttgcaaa gacacatcac ataccaaaag
    28021 ataaacctag aaaaagcaag cctagaaagg agtcataaag aagattaaac atgaggaaaa
    28081 gcccccatat catcaagtaa tcccaacgcc cacttcgttt tgcaactaga aataagatta
    28141 gatggtagag tacatgaaag actaaaaatc caataaaact tggata
//
LOCUS       DAVKDZ010000022        25351 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000022 DAVKDZ010000000
VERSION     DAVKDZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25351
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.022"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(144..1778)
                     /locus_tag="VWZ30_000932"
     CDS             complement(144..1778)
                     /locus_tag="VWZ30_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334695.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMIICFSNIAIHFPFEEMPVYFSFMVAILLVIAFSTSLTAFYNVFYESK
                     DLASYSPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLALYISLAPSLWLGLPLMLL
                     SLTLLFVSVTLVTVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFVLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTIKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFIALFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPILTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAISLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1759..2508)
                     /locus_tag="VWZ30_000933"
     CDS             complement(1759..2508)
                     /locus_tag="VWZ30_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9334696.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVED
                     LRKDYPDQSLESIYLSLAGRKEEVADASQGH"
     gene            2687..4141
                     /locus_tag="VWZ30_000934"
     CDS             2687..4141
                     /locus_tag="VWZ30_000934"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEU9334697.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADNREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFDASRAGSNYNLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEVLDRLLTIR
                     KDLAYGEQTDYFDDANCIGWVRSGAENQSPIAVLISNDQENSKSMFIGQEWAKQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSAASVSVWATNTI"
     gene            complement(4231..6849)
                     /gene="alaS"
                     /locus_tag="VWZ30_000935"
     CDS             complement(4231..6849)
                     /gene="alaS"
                     /locus_tag="VWZ30_000935"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU9334698.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGSSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVEAVSEGTASLIFAETPFYAEMGGQVA
                     DHGQILNASGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLVIDTNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIVDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(6871..7356)
                     /locus_tag="VWZ30_000936"
     CDS             complement(6871..7356)
                     /locus_tag="VWZ30_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEU9334699.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7631..7870)
                     /locus_tag="VWZ30_000937"
     CDS             complement(7631..7870)
                     /locus_tag="VWZ30_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334700.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8131..9201)
                     /locus_tag="VWZ30_000938"
     CDS             complement(8131..9201)
                     /locus_tag="VWZ30_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9334701.1"
                     /translation="MKKLYSFLAGIVAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLEQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKLEGLENIGLEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDPQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNAAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDA
                     AYAFINFMLKPENALKNAEYVGYSTPNLPAKELLPEEKKEDKTFYPDAETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9198..9971)
                     /locus_tag="VWZ30_000939"
     CDS             complement(9198..9971)
                     /locus_tag="VWZ30_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9334702.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLIVTQTFFLAFLSAFIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTKLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(9968..10774)
                     /locus_tag="VWZ30_000940"
     CDS             complement(9968..10774)
                     /locus_tag="VWZ30_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9334703.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGHRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10755..11912)
                     /locus_tag="VWZ30_000941"
     CDS             complement(10755..11912)
                     /locus_tag="VWZ30_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9334704.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDILLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILSGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(12072..12977)
                     /gene="murB"
                     /locus_tag="VWZ30_000942"
     CDS             complement(12072..12977)
                     /gene="murB"
                     /locus_tag="VWZ30_000942"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU9334705.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSSITLEREVRILGESK"
     gene            complement(13089..13841)
                     /locus_tag="VWZ30_000943"
     CDS             complement(13089..13841)
                     /locus_tag="VWZ30_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEU9334706.1"
                     /translation="MTIHIIIIMLLLLVFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLVYIIYGLAMAAFFEETARLVFFKCLEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLSQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(13851..14582)
                     /gene="budA"
                     /locus_tag="VWZ30_000944"
     CDS             complement(13851..14582)
                     /gene="budA"
                     /locus_tag="VWZ30_000944"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEU9334707.1"
                     /translation="MDKNVQEPAKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGTGEQPEIVEVSPDTLIPYAAVVPHQAEVIFRQRFEMTD
                     KELEERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     GGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14724..15995
                     /locus_tag="VWZ30_000945"
     CDS             14724..15995
                     /locus_tag="VWZ30_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU9334708.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFDQALYCKTNQLVLTSGTQQALFILSQISFP
                     SQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(16015..16830)
                     /locus_tag="VWZ30_000946"
     CDS             complement(16015..16830)
                     /locus_tag="VWZ30_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9334709.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     CLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(16987..17640)
                     /gene="phoU"
                     /locus_tag="VWZ30_000947"
     CDS             complement(16987..17640)
                     /gene="phoU"
                     /locus_tag="VWZ30_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9334710.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(17652..18410)
                     /gene="pstB"
                     /locus_tag="VWZ30_000948"
     CDS             complement(17652..18410)
                     /gene="pstB"
                     /locus_tag="VWZ30_000948"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9334711.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(18423..19226)
                     /gene="pstB"
                     /locus_tag="VWZ30_000949"
     CDS             complement(18423..19226)
                     /gene="pstB"
                     /locus_tag="VWZ30_000949"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9334712.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(19237..20121)
                     /gene="pstA"
                     /locus_tag="VWZ30_000950"
     CDS             complement(19237..20121)
                     /gene="pstA"
                     /locus_tag="VWZ30_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9334713.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(20111..21028)
                     /gene="pstC"
                     /locus_tag="VWZ30_000951"
     CDS             complement(20111..21028)
                     /gene="pstC"
                     /locus_tag="VWZ30_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9334714.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(21028..21906)
                     /gene="pstS"
                     /locus_tag="VWZ30_000952"
     CDS             complement(21028..21906)
                     /gene="pstS"
                     /locus_tag="VWZ30_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU9334715.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTMLEGRQ"
     gene            complement(22480..23784)
                     /locus_tag="VWZ30_000953"
     CDS             complement(22480..23784)
                     /locus_tag="VWZ30_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEU9334716.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(23785..24558)
                     /locus_tag="VWZ30_000954"
     CDS             complement(23785..24558)
                     /locus_tag="VWZ30_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU9334717.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(24548..24826)
                     /locus_tag="VWZ30_000955"
     CDS             complement(24548..24826)
                     /locus_tag="VWZ30_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEU9334718.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKEEGKISLGK"
     gene            complement(24826..25227)
                     /locus_tag="VWZ30_000956"
     CDS             complement(24826..25227)
                     /locus_tag="VWZ30_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU9334719.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
BASE COUNT         7597 a         5796 c         4900 g         7058 t
ORIGIN      
        1 aaatagaact atgttatatt tcttattcaa aacattcctc cctatatatt tttgattacc
       61 aatcttaatc atttacaact acattctaac aaactataaa agcgtttgtc gaattgaatt
      121 tatcaagcaa gcgaccaacc agctcatctt ttttctattt ctgccaatat gcgtgacagg
      181 tagtaatgat agccaaaaat agcaagagca agcaagacga taagagctcc tactcccaag
      241 ctgatggcaa ggatagggga gagagactga accaagaata tgctcccaat tacaagagcc
      301 atcaggattg cactataaat aaacaataaa actatggcga ctatgccatt tgaacgattc
      361 accaggtccg taatgctact ccaattggtt gacagatttt taacgtcctt aaagtaatgg
      421 tggcaagaaa ggatgacact ggcaatgatc cagactacaa gaaggtaaat catcgaaagg
      481 atggacaagc ctagatatag agaaagaccg agtaaagtca gaattggtaa aaaggactga
      541 acagcaaaga taatccaaaa tttcactttc acataacgag caaagtcaaa gggtaaactc
      601 ttcagaaaat caacattttc cctctccaag gacaaggcaa ttgaatgcag gctggtgata
      661 ttgttattga caactgctat aaagagagct ataaaaaaca agggtaacca gtatggagga
      721 tgaatgtctg gaactatctg agaatctcgg attttggaaa tcagactgat catcatgaga
      781 taaggaagga aagcacttgt aaaaagcact gtaatcacgc cagtcccctg tcccaagagg
      841 gtgaggtggt agcgtaaaac catacggaaa aagccctttt taatagttga aatcctctcc
      901 ttgctgcgac gttctttttt gaccttctcc tcactgttaa gcaggatcac gtcataaaaa
      961 cgaggaagga ccttcttttt ggtcagataa agcaggaaga gagttagtcc tatccaagcg
     1021 agcagaccca ctaaggcttc tgtcgaaaaa ggctccactg ctattttgta aaagatatga
     1081 agaggataaa ggacaaatgg aatgtctcta actttgtcaa caatacttcc aaaagtcgac
     1141 tgaagaaaga agataaatat taaaggtatg agaactccta tcccaatcat cacattcgaa
     1201 aaaatagact gatactttct gaagacccta gtctgagcca agaaatgtac tgccactacc
     1261 gtcactaaag taacagagac aaataataag gtcaaggaca gtagcatcaa aggcaaaccc
     1321 agccaaagag aaggagctag actaatatag agggctagaa aataagctag gattggtaca
     1381 attccagtta gagctggcaa aaggacagac agtcctttag caattataat ctctgattct
     1441 ttaaaagcat agggactata ggaggccaaa tccttactct cataaaaaac attgtaaaat
     1501 gcagttaaag aagttgaaaa ggcaatcact agtaaaatag caaccatgaa actaaaataa
     1561 acaggcattt cctcaaaagg aaaatgaatg gctatattac taaaacagat gatcatcaag
     1621 agactggaaa aaatgtaaga acttaagact ctagcggaaa catttacttt tttcccagga
     1681 ttcttagcct gcttctttcg tagattagcc agattagctt cttgagatga ataaaggata
     1741 ttgatatcaa ctaatttttt aatgaccttg agacgcatcc gcaacctcct cttttctacc
     1801 agcaagacta aggtagatac tttccaaaga ctggtctggg taatcttttc tcaagtcctc
     1861 tacactacca caataaatca aatgcccctt tttcaaaatg gcaatccgat cacagacttg
     1921 ctctgccacc tctaggacat gagttgaaaa caagactgtc ttcccttttt gtgcatgttc
     1981 cttcatcatc tgtttcaaat caaaggcagc ctggggatcc aaaccagtca agggttcatc
     2041 caagacccaa atatcgggat cagacaagag tgctccgatg acaaagactt tctgacgcat
     2101 tccgtgagaa agagtttcaa taacctgata gcgattttca gcaaaatcaa aaacgttcaa
     2161 tagcctagct agactagcct ccaagtcaga tctactcaga tcataggatg aggcgatcaa
     2221 ttcccaaaat tcattggccg ttaagcgtaa aaataagtca ggcgagtctg ctacgtagcc
     2281 aatctttcgt ttaatagcca agcgattttc cgataactcc tgaccgtcta ccaaaatacg
     2341 accactgctg ggtgaaatga tactgactag ggattttata gtggtcgatt ttccagcccc
     2401 attatgacca atcaagccca taatctctcc attttcaatc tgcaaattga gattactcaa
     2461 ggcctcttta tcaccataca acttactaac attttgaaat tcaatcatgg gacaactcct
     2521 atctttatct gtattataca tcgctatact agcactttga tacaaaaaaa tcaatacttt
     2581 attttaagta gttaaaatag tttccttcta tcttacgcaa acgtttgctc cgagagatac
     2641 tttatgatag aataaagaac aagattgaca agtaagagga aacatcatgc aaaatcaaac
     2701 actcatgcaa tactttgaat ggtatctgcc ccacgacggt caacactgga cgcgtctggc
     2761 tgaaaatgct ccacacctag ctcatctggg gatcagtcac gtctggatgc caccagcctt
     2821 caaggcaacc aacgaaaaag atgtcggcta tggggtctat gacttatttg acttaggaga
     2881 gttcaaccaa aaagggactg tccgcaccaa gtatggtttc aaagaagact atcttcaagc
     2941 cattcaagcc cttaaagcac agggaattca acccatggct gatgtagttc tcaaccacaa
     3001 ggctgctgcc gataacaggg aagcctttca ggttatcgaa gttgatcctg tagaccgtac
     3061 agttgaactt ggagaaccct tcaccatcaa tggctggact agttttacct tcgatggtcg
     3121 ccaagatacc tataatggct tccactggca ttggtaccac ttcaccggta cagactacga
     3181 tgccaaacgc agtaaatctg ggatttatct gatccaaggg gacaacaagg gctgggctaa
     3241 cgaggaattg gtcgataacg aaaacggaaa ctacgactac ctcatgtatg ccgacctaga
     3301 ctttaaacat cctgaagtca tccaaaacat ctatgactgg gctgattggt tcatggaaac
     3361 gactggtgta gctggtttcc gtttggatgc cgttaagcat attgactctt tctttatgcg
     3421 caacttcatc cgcgatatga aggaaaaata cggtgacgat ttctatgttt ttggtgaatt
     3481 ttggaaccca gacaaggaag ccaatctgga ctatctcgaa aaaacggaag aacactttga
     3541 ccttgtcgat gttcgtctcc accagaatct ctttgatgct agccgagcag gttccaacta
     3601 taaccttcgt ggcattttca cagatagcct ggttgaactc aaacctgaca aggctgttac
     3661 ctttgtcgac aaccacgata cccaacgagg acaagctctt gaatctactg ttgaagaatg
     3721 gttcaaacca gcagcctatg ccctcattct attacgccaa gatgggcttc catgtgtctt
     3781 ttacggcgac tactatggga tttcagggca atatgctcaa gaagatttca aagaagtcct
     3841 tgaccgcctc ctaaccatcc gaaaagattt ggcctatgga gagcaaacag actactttga
     3901 tgatgctaac tgtatcggtt gggtacgttc aggcgctgaa aatcaatccc caatcgcagt
     3961 cttgatttca aatgaccaag aaaacagcaa gtcaatgttt atcggccaag aatgggctaa
     4021 acaaaccttt gtagatttac ttggaaacca ccaaggtcaa gttacaattg atgaggaagg
     4081 ttatggacaa ttcccagtct cagctgcttc ggtaagtgtc tgggcaacca atacaatcta
     4141 atagctaatg ataatcaagc caggtccaat cggatttggc ttttttgcat tcacaaaaag
     4201 acctacccaa atggatagat ctttactcga ttacaattta cctgctactg catccaacaa
     4261 ttcttgaatt ttagcttggt tgcttcctcc tgccatggcc atgtctggtt ttccaccacc
     4321 acgtccatcg acgattggtg ccaattcttt gacaaggttt cctgcatgaa ggtcttttgt
     4381 cttgcttgct acaaggacat tgactttgtc accgatagcg gcaactagga caagaagatc
     4441 agagtagtct ttttgtttcc aattatctgc aaaagtacga agggcacctg catctgatac
     4501 agaaacttgg ctagcaatgt aacggtgacc gttgacttcc ttcacatcct tgaagatatc
     4561 gcctgcggct gcagctgcgg ctttttcttt caattcagca ttttcttttt gaagttgacg
     4621 aagttgttct tgaagtcctt ctaccttgtg aggtacttcc ttgacttgag gtgctttcaa
     4681 ggttgctgcg acagctttaa gagcgtcttc ttgttcacgg taagcttcaa aggcttcctt
     4741 accagtcact gccaagatac ggcgagttcc tgatccaatc ccttcttctt tgacaatctt
     4801 gaaaagacca atttcagaag tgttgccaac gtgggtacca ccacaaagct cgatagagta
     4861 gtcaccgata gtaacaacac ggacttcctt accgtatttc tcaccaaaga gagccatagc
     4921 tcccatttct ttagcagtgt caatatccgt ttcaactgtc ttcacttcaa gagcttccca
     4981 gattttctcg ttgacttgct gttcaatcgc acgcaattct tcagcagtta ctgcttggaa
     5041 atgcgtaaag tcaaagcgaa ggaattcaac ttcgttaaga gatcctgctt gtgttgcgtg
     5101 atttccaagg atattgtgaa gggcagcgtg aagcaagtga gtcgcagtat ggtttttcat
     5161 gacacggtga cgacgatttg tatcaattac caaggtatat tcttggttca aggcaagcgg
     5221 tgcaaggact tcaactgtat gaagagcttg tccgtttgga gctttctgaa cattggtcac
     5281 agtagccacg accttacctg acgcattcaa gatttgtcca tggtcagcta cctgtccgcc
     5341 catttcagca tagaatggcg tttccgcaaa gataagtgag gcagttcctt ctgatacagc
     5401 ttctacttct gcattgtccg ccacgatagc taccaattta gaagacaatt ggctagcatt
     5461 gtagttgaag acactttcta cagtgatgtt ttgaagagtt tcattttgca tacccattga
     5521 gctacccttg acagctgacg cacgcgcgcg ttcttgttgt tctttcatgg ctgcttcaaa
     5581 accttcacgg tctacagtca taccagcctc ttcagcaatt tcttcagtca attcaactgg
     5641 gaatccgtat gtatcataga gtttaaagac atcttgacca gcaatgacag attgaccttt
     5701 ttctttcaag tctgccacaa tgccttgggc aaagtgttga cctgagtgaa gggtacgggc
     5761 aaatgattct tcttcgctct taacgatttt ctcaataaag tcacgtttct caagcacttc
     5821 tgggtagtag ctttccatga tttttccaac agttggaacc aatttgtaaa ggaaaggctc
     5881 gttgataccc aatttttgac catgcataga agcacgacgg agaagacgac gaagaacata
     5941 accacgacct tcatttcctg gaagggcacc atcaccgata gcaaatgaaa gagaacgaat
     6001 gtggtctgcg ataaccttga agctcatgtt gtcgccatct tggtcataaa ccttaccaga
     6061 caatttctcg acttcacgga taatcggcat gaagaggtcc gtttcaaagt tggtcttagc
     6121 cccttggata acagccacca aacgctccaa accagcgccc gtatcaatgt tcttatgtgg
     6181 caattccttg tattcgctac gaggaacagc agggtctgcg ttaaattgtg acaaaacgat
     6241 gttccagatt tcaatataac ggtcgttttc aatatcttct gcaagcaggc gaagaccgat
     6301 attttctggg tcaaaggctt ccccacggtc aaagaagatt tctgtatctg gtccagaagg
     6361 tcccgcaccg atttcccaga agttgtcctc aattggaatc aagtgacttg gatccactcc
     6421 cacttcaatc cagcggttgt aagaatcttt atcgtctgga tagtaggtca tgtaaagttt
     6481 ttcagcaggg aaatcaaacc attcagggct tgtcaaaagc tcataagccc aagtgatagc
     6541 ttcgtcacgg aagtaatccc cgatagagaa gttccccagc atttcaaaca tggtatggtg
     6601 acgcgcagtc ttccctacgt tttcgatgtc gttggtacgg atagcctttt gggcattggt
     6661 aatacgtgga ttttcaggga taatggtccc gtcaaagtat ttcttaaggg ttgctacccc
     6721 agagttgatc cacaaaagag ttgggtcatt tacaggaacc aaacttactg atggttctac
     6781 tgagtgacct ttggtcgccc agaaatcaag ccacatttgg cgtacttgtg cactagatag
     6841 ttgtttcata ttgtctcctt attcacttgt ttaatgtgat tggctttcca gcatttccac
     6901 atagtcaatc gcgacacaga gggaaatgac taggtctgca taagcgtctt caagaaccgt
     6961 tacggtatag gtagaggtca gatggaagag ttccttctta atttccgcaa tcaactgatc
     7021 gcgatcatcc agcaatttga aattcaaatc ccagatattg ccctcgatac gaagacctag
     7081 attatcaaac tcatacttat ctcgccagaa ggtcaacttc ttacgaatga caaaactcga
     7141 gccatcccga agctgaatct caaaacgagg aagcaaggtc aagatttctt tactaatctc
     7201 actgacttgt tcaccagccg catcatagat ggtaaaggtt ttaggaatct taaaaaatga
     7261 tccctccacc tgataggcaa tttctcccct gtcatccttg atagcgaagc gttcgcctcc
     7321 aagacgaaac ttttgtttga caagaaatgt tttcatcaac acctccaaaa atcaaaagac
     7381 aagctcatat cacgaagggc gaaaaaccgc ggtaccacct tcattcaatg aacttgtcat
     7441 tctcttgttc ttatgcaatt gtatgattga gtagcatgac ttcctagctt agatggctcg
     7501 cagcaccgcc atttctctgg actaagacaa gtgaaaatca attctcaact ctcttattat
     7561 aacgtttttt taagcttgcg tcaactggaa atgatctccg ttgaattaga ccaattccct
     7621 acatctctga ttactttttc aggatatatt ttttcttact gccatttttc tttttatccc
     7681 aaattttcat attactaaac acagctacta gaatatttcc aaatataaag gtgcctatca
     7741 cccaatatat ggactcagtt gttaggtatt gtcgatccaa gccatccttt aaatggaata
     7801 gtataacagt ttggttaaca atcataaagg ttggccagaa actttttttg aaaaaagtag
     7861 acattttcat tatttgttgc cgctttctgt aaggttaata ctcaataaaa atcaaaaagc
     7921 aaactaggaa gctagcctca agctgtactt gagtacggca aggcaacgct gacatggttt
     7981 gaagagtata ggcttagtat actactaggc aagcaaataa gcaaataagc aaataaacaa
     8041 ctagaataga aaaagatagg gctctaaaaa ctgacttcta ttccttaaaa acgaaccagc
     8101 ttgactgatt cgtcttctta cgtttatctc ctacttccga tacattttaa actgtaggaa
     8161 gaggtcgcta tattttcctg tccatttatg gtcaaatttc tcataaacct ctaggtgttt
     8221 catggtttca gcatcaggat agaaggtctt gtcttctttt ttctcctctg ggagtaattc
     8281 cttagctggt aggtttggtg ttgaatagcc gacatactcc gcatttttga gagcattttc
     8341 gggtttcaac ataaagttga taaaggcata ggctgcatct tggtttttga ctgttttggg
     8401 aatgaccata ttgtcaaacc aaaggttgct ggcttctgtc ggtaccacat agcgtagatt
     8461 ttcatttttt tctagcattt ggctagcttc cccagagaag gtcacaccga tggcagcatt
     8521 gttctgaatc atgtagccct tcatctcgtc ggcaacgata gccttgatat ttggagtcaa
     8581 tttatagagc ttatctactg tctcttccaa ctgctgtgga tccttggagt tgaggctgta
     8641 gccaagggaa ttaagtccta gtcccagcac ctcacgcgcc ccatcaaaga gcataataga
     8701 gttcttatac tctggcttcc agaggtcatc ccagtgctca ggcgcttcat ctaccatagt
     8761 ttcattgtag acaattccca gggttcccca gaagtaaggg atagagaatt tatttcctgg
     8821 gtcaaaggac tggttgagga actctaatcc gatattttca agaccttcaa gttttgaata
     8881 gtcaagcggt actaagaggt cttcgtcctt catcttgtta atcatgtatt cactaggaat
     8941 ggcaatatcg taggtcgttc caccctgctt gatcttggta tacatggctt cgttggagtc
     9001 aaaggtctca tactggactt ggatgcctgt ttcttctgta aattgctcca agagttctgg
     9061 atcgatatag tccccccagt tgtagataac cagtttttga ctatctcgac tattgatttt
     9121 actatcgaga tgagtcgcaa ttccccacaa gacgaggata atggctacaa ttcctgctaa
     9181 aaatgaatag agttttttca tgcttgctcc tccttctcac gtgagataaa gtaatatcca
     9241 acaactagga taatactaaa gagaaagaca agggcagaca gggcattgat ttctagcgaa
     9301 atccccttac gagcacgaga gtaaatctcg actgacaggg ttgaaaagcc atttcccgtt
     9361 acaaagaagg tcacagcaaa gtcatctagc gaataggtga aggccatgaa ataaccagca
     9421 atgatagatg gagtcaggta aggaagcata atttccttga acatctgaaa ttgactggca
     9481 ccaaggtcat aggccgcatg aatcatatcg ccattcattt ccttgagacg aggcaagacc
     9541 atcaagacca cgataggaat ggagaaggcc acgtgactag atagaacggt caaaaagcca
     9601 agtgaaaact tgagtttggt aaagaggatc aagaaactgg caccaatcat aacgtcaggc
     9661 gcaaccatga ggatattatt gagtgaaaga aaggcttctt gatatttctt acgagactgg
     9721 tagatgtaaa tagcaccgaa agtcccgata atggtcgcaa taaaggctga taaaaaggcc
     9781 aagaaaaagg tttgagtcac aattaacata aggcgaccat cgccaaacat ggtttcaaag
     9841 tgagtccagc taaaacctgt aaagctattc atatcatcac cagcattaaa ggcatagcca
     9901 atcaaataaa aaatcggcag gtagaggaca agaaagacca gtcctagata aaggttggca
     9961 aattttttca tcgttctctc ctttccttgg tcacccacat ggtgatgaac atggtcaaga
    10021 tgagaattac accgatggtt gaacccatac cgtagttgtc attggttagg aaattctgtt
    10081 caatagccgt ccccaaggtg ataacgcggt gcccaccaat caaacgggtc agcatgaaga
    10141 gactcaaact tgggataaag acggattgaa ccccacttct cacaccgttc atcgatagag
    10201 ggaagatgac atgacggaag gtctcccact tggtcgcacc gaggtcatag ctggcattga
    10261 tgagattgtt atccatatcg tccaagacat tgaaaatcgg caaaatcata aagggaagct
    10321 cgatgtagct tgcgacaaag ataaaggaga aatcggtaaa gagcaactgt tgcgaaccaa
    10381 ttccgataaa ttccaagaat tgattaatag agccattctg accaaaaatc ccgataaagg
    10441 cataggcttt aaggagcaaa ttgatccagg ttggcaggat aatcagcatg agccagagtt
    10501 gacggtgctt gagacgggtc aaaaagaggg ctgtcggata gctgataagc aaggtcacaa
    10561 aggttacaat ccctgcatag agtacagagt tgaaactcat ttttaggtag gtcaagtttt
    10621 gtgacgcaaa gtaagatttg taattttcta aactgaactg gccttcgatg ttgaaaaagg
    10681 attgaccgaa aatcaagacc aagggtgcca gaacaaagag ggcaatccaa agcatgtagg
    10741 gcactacaaa gagtttagag cttgttttct tcatctcttt cctcctcgat tgcattgatc
    10801 aaacctgctt cttgctcttc gatttccacg tattcttcga tacgagcatc gaactcttct
    10861 tctgtttcgt tgagacgcat gatatggatg tcttctggtt caaagtccag accgatttcc
    10921 tcaccaacaa tagccttacg ggttgagtgg atcatccatt cattcccaag ctcgtcatag
    10981 gcgataattt catagtgaac tccacggaag agctgggtat ccaccttaac ttggagcttg
    11041 ccttcttcag gaagggtaat gcgcaagtcc tctggacgaa taacgacctc aacaggttca
    11101 tttggcttca tccccccatc gactgcttcg aagcgtttgc cgttaaattc gaccaagtag
    11161 tcctcaatca tggttcctga caagatattt gactcgccga taaaggtggc aacaaagtga
    11221 ttgattggct catcgtagat atctactggt gttccagact gaacaatctc gccatcattc
    11281 ataacgaaaa tccagtcact catggccagc gcttcttcct gatcatgagt aacaaaaaca
    11341 aaggtgattc ccaatcgttg ttgcagttca cgcagttcgt actgcatgtc tgttcgtaat
    11401 tttaagtcca gcgctgacag aggttcgtcc aacaacacca cacggggttg gttgatgatg
    11461 gcacgggcaa tagccacacg ctgacgttgt cctccagaaa gtttgcggat ggaacgtttt
    11521 tcataccctt ccaactgaac catcttgaga acttccgcta cgcgttgctc gatttctttc
    11581 ttgtcaattt tacgcaagcg aagtggaaag gcaacatttt caaacacatt catatgcgga
    11641 aacaaggcat aagattggaa aacggtatgg acgtctcgct tgttggttgg gatgtcattg
    11701 atacggacac catccagcaa aatatctcct gtcgtcgcat ccagtaaccc tgcaataatg
    11761 tttaggatgg ttgatttccc cgaaccagat gcacctagaa gggtgtagaa ttttccttct
    11821 tccaactcaa agttgatgtc tttgagaacc ttggtgttgc tgtcttcaaa aactttagag
    11881 acgtttttga attcgataat tggttttttc aattggcata aattccttct ttttcataga
    11941 ttaaccgatc ggggctctgt caggtcccca ctacctcttg cagggagtaa aaccacctgc
    12001 atacatcttc gctaccgata ggctttcacc ccctttccat gacatagcag cagcttttca
    12061 aatacagctt cctacttgct ttcacccaag atccggactt ctctttcaag cgtaatactt
    12121 gagtgttcct taactttttc gataaccgat tggatcaagt cctcgtagtc tttggccgtt
    12181 ccatctgcga cattgatcat aaatcctgca tgcttttctg acacttctac gccaccgata
    12241 cgatagcctt tcaagccagc ttctgaaatt aactgacctg caaaatgccc gactggacgc
    12301 ttaaagaccg agccacaaga tgggtattcc aaaggttgct tgagttcacg taggtgcgtc
    12361 aagcggtcca tttcctgctt gataacctga tgggttcctg gggctagggc aaatttaact
    12421 gacaagacaa ctgcaccaga ctcctgaata gctgaatggc ggtaaccaaa agccaagtct
    12481 ttagcagaca gggtttcgat ttctccatcc ttggtcaaga ccttacaaga ctgcaagatg
    12541 tgagcaatct cgccaccata ggcacccgca ttcataaaga cagcaccgcc aacgcttcct
    12601 ggaataccac aagcaaactc aaagccagtc aaactatgac ggagggcaat gcgagttgtt
    12661 tcaatcaagt tagccccagc ttctgcttca atggtatagc catcaacaga aacgttattg
    12721 agcttgtcac acaagatgac aaatccacga atcccaccat cacgaacaat gatattgctt
    12781 gcattaccaa gaaccatcca agggatattt tcttggttgg caaatttcac aacgcgagcc
    12841 aactcaaaac gatttcgtgg aaagaccaaa taatcagcct ctccacctac ttttgtataa
    12901 ctatagctat gcaagggttc cttaaaacgg atatcaattc cttctaagat ttcaagcatt
    12961 ttttctctta cagacatgtc actcttcctt ttacaaaatt cattccatta taccattttt
    13021 agagacattt gacgaccata aaaatacctt gtttggattt tgcataagaa aaagaggttc
    13081 cccccttttt atgatttttt acaaaagatt tccttggttc cataggcgac cagaacgagc
    13141 tccagtgcta gaatcacttc aaccaagact ggatttgtca accagcctac ttgggaaaga
    13201 gatggtgcca gatcaaagaa ggcatgcaag ccataggctg ctaggagata aatccatttc
    13261 ttctggcgaa cagcttggta aacccaaact gtcaaaagta attggaaacc aagcgccaag
    13321 attcgctcaa aaccaagcaa ataaatctgc cagaccgaaa gtgactgaat ggtttttaac
    13381 atattttcag acagtaattg cataacctgt ggattctgag tttgaactgc cgaaagaaca
    13441 atgtaaagat tgagtaaact agtaaggcct agaaaaatca actccaagcc accatgccca
    13501 agtccatagg ccaaggcgtc tgccttttcc aaacttctct ttttctccaa gcatttgaag
    13561 aaaacaagac gagccgtttc ctcaaaaaat gctgccatgg ctaggccata aataatatag
    13621 acaagcggat gatcttgcaa gagggcaata ctaccgtctt tttgaggatg caaaatcaag
    13681 atatgcacca gtttttccaa aatctgcgaa gagacaaaga aagcgactgc acccaagccc
    13741 aagacagcta gattaatctg gtatttcttt ttggcaaacc aaatgcttcc tatcagaaaa
    13801 actagcaaca gcaacatgat aataatgata tgaatggtca ttttcttctc ctattctgcc
    13861 ttttcaatat cttttttcat ctcgtcaaca ttgaacttag caaacaagta ttgacggtct
    13921 tggactggga aacgttggtc caactggtca actgctccca cctcgataat gccttccttg
    13981 atgacaaaat ccatgacatg tccaccgaag gtcaaatcat ctgatatgaa gtgcagatgg
    14041 tagcctgcca cactgacccc atggaaaatc tcaggcgtcc agaaaccaac aatagtcccc
    14101 ccaacattgt cacgactata ttccggttga tgggttgcga catcagcaaa cttggtatcg
    14161 ggtgttgact taggaatcat acgcacatgc atatgtgaaa attctccccg aatcttgata
    14221 gaacggaaaa gattttcccc atcataataa gactcaattc gttcttccaa ttccttgtct
    14281 gtcatctcaa agcgctggcg gaaaatgacc tctgcctgat gcggtactac tgctgcataa
    14341 ggaataaggg tatctggtga cacttccaca atttctggct gttctcctgt tccttttgcc
    14401 tgataagcct tgccatctaa gacaatcaat tccccatcaa tagaatccaa ggttcccaaa
    14461 ccaaggtcac catgctctag caattctccc actgtcatgg taccaccata aaggccagcc
    14521 atcaaggctc caagagtatt gtattgaaat aatttcgctg gttcctgcac gttcttatcc
    14581 attcactttc ttgtctgtta ttctataaat gttactccta agtataccac atttgcccct
    14641 agatgtgaac gagagaaacg ctctagacat tgccaagaag gaaaaaaaag ggtacaatgt
    14701 aacaaaatca agggaggtct ggaatgaaga aacaaagcaa gtacaaagag gtcgtttcct
    14761 atctgaaaaa tggtatcgag tctggacgat ttccgacggg tagtcgcctg ccttctatcc
    14821 gtcaactgag ccttgacttt cactgcagca aggacaccat tcaacgagcc ctgctggaat
    14881 tacggcacga acaatacctc tatgccaagc ctcagagtgg ctactatgta ttagaacaag
    14941 ggcaacatca agacctagaa atcgaggtta ccgacgaaca tgccagtgcc tatgacgatt
    15001 tccgactctg tgtcaatgaa accttgattg gccgagaaaa ctacctcttc aactactatg
    15061 acaatcaaga aggattagaa gacctaagac aatccattca caaactcctc tttgaccaag
    15121 ctctctactg caagactaac caactagtac tgacttctgg aacccagcaa gccttgttta
    15181 tcctatctca aatatccttt cctagccaag ccaaggaaat cttggtggaa cagccaacct
    15241 accatcggat gaatcgcctc ttgattgcac aggggctgga ctatcaaacg attgaacgag
    15301 gcattgatgg gattgacttg gaggagctgg aaggccactt caaaacagga aaaattaaat
    15361 ttttctacac cattccccga tttcactatc ctctgggaca ttcctattct gagcaagaca
    15421 aacgatctat tcttaactta gctgccaagt atgatgtcta tatcgtagag gacgattatc
    15481 tgggtgattt ggactccaag aagggccaaa ccttccacta tcttgataca gaggagcatg
    15541 ttatttatat caagtccttc tcgaccagcc tttttcctgc ccttcgtatt acagcactca
    15601 ttcttccaaa tgctatcaaa gaagcatttg tggcctacaa aaatatccta gactacgaca
    15661 gcaacctcat tatgcaaaag gccctgtcac tctatattga cagtcaattg tttgaaaaaa
    15721 atcgtttggc tcgcttgacc aatcatgaat cttaccaaaa acaaatcgaa gaaaggataa
    15781 ctaaaacacc ttgtcccctc cctcattatc ccctacacga tggtttattg ctagacctga
    15841 gacagtatcc taaaatcgcc agtctcaaac acagtcaact gggcttggac ttctttgaag
    15901 aggcctattt aagcacctgt ccttatcaat ttgccaaggt gtccctagac aatctggaaa
    15961 atgttttaaa ctatttaaaa gcagaattgg aatgacatcc aactctgctt tttcttattc
    16021 ttcaacttca gttagacttg ctgctttttc aagatagctt tggtaagttc cttcatcctt
    16081 aagtttttgg ataaccttgt ctaccacttc tttcaaatca tcactatttt ttctaagagc
    16141 aacggcattg gcgtcgccgt ccttcatctt caagcagaca gttgcgacag ctaagccagc
    16201 gtttttagca gcataactaa gtgcaacagg ttcatccata tgaacagcat ctacttttcc
    16261 agcctgcaat tcattgactg cttcacccat attagttagg gaagttaatt gagcttttgg
    16321 caattgttcc ttgaccattg attctggaac agtccctttt tgggctgcaa tattagcact
    16381 ttctaggcta gttaaatcct tgtatttttc cacatcagcc ttacgaacca agaaactaat
    16441 cttgttttca tagtatggga ttgaaaaatc aaagacttct tttctctcgt cagtagcact
    16501 aattcctgca actgctaggt cagccttacc agtttgaaga ctggtcaaaa cattgtcaaa
    16561 actcatgctt gagatttcca acttaacccc aagttcatca gcgatagcct gagccatgtc
    16621 gatgtctgca ccgactacct ggttctttcc atcaaccaat gattgaaatt caaagggtgc
    16681 atagtcagga ctggttgcca caactaattt ccccttttgc ttaatggcct caacagctga
    16741 ctgagaacca ttagaacctg actggcaagc tactaagaag aaacttgcaa gaaaattaca
    16801 taaaacaaat atccattttt ttgatttcat aaataaacaa cctctctgtt aattttgtat
    16861 aattataacg atatccaagt tacttgtcaa gtgtttttta aatttttatc tcaaaaatat
    16921 ttttttgttc aaaaaaagga gccatcagtt gattccaagc tcctttttat acagaattaa
    16981 actattttat agttcgacaa tcttacctgt ttcaaagtag acaacccatt cacagatatt
    17041 tttagcatag tcaccgatac gctccaagta ggaaataact tggaaataat cacgacccgt
    17101 aacaatggct tctggatttt tcttaatctc ttcagtcgca aggtcacgga tagtttcaaa
    17161 atagtggtta atttgctcat ccatggaggc cacccggtat gcgtcgtcaa cagaaccatt
    17221 aagataaaga tcaagtgctg cttcaacaac gcttttaact tcacgtccca tttttttaat
    17281 ttcttcctct acagctggga tgcgttcttc ccccttcata cggatggttg cctgggcaat
    17341 ggctacagcg tgatccccca tacgctccac atctgataca gccttaagga cagtcaagac
    17401 tgtacgcaaa tcttgagaga ctggttgttg gagtgcgatc atttcaaatg atttcttttc
    17461 cagtttcact tcgtattcat ttacttctgc atcatcttcg atgacctctt ttgccaggtc
    17521 acggtcatgc gtgacaaaag cacgtaccgt acgattgatt tgtgagagca cttcttgtcc
    17581 catagcgtag aactggttat gtaatttctc taaatcttct tcaaattgag atcgtaacat
    17641 ctttcatctc cttatccaaa ttttcctgta atatagtctt ccgtttcctt gtgttgggga
    17701 tcaaggaaca tctgcttggt atcattaaat tcaatcaaat ctccatctag gaaaaatcct
    17761 gtcttatcag agatacgtga agcttgctgc atggaacggg ttaccagaag catggtgtac
    17821 ttgtctttta gaccatacaa ggtttcctca attttaccag ctgaaatcgg atccaaagcc
    17881 gaagttggct catccaagag gatgatttta ggactagttg ccaagacacg ggccacgcag
    17941 acacgctgct gttgaccacc tgacaatcca atagctgaat catatagacg atccttgacc
    18001 tcatcccaga tagaggcacc ttgcaaggct ttttctacgg cttcatccag aacctgctta
    18061 tccttaattc cattgatacg aagcccgtag acaacattct catagatagt catagggaaa
    18121 ggattaggtt gttggaaaac cattccgatt tccttacgta attcaaccgt atctgtacgc
    18181 ggactgtaga tgttgtgacc attgtacacc acggatccag ttgtggtcac ctctggattg
    18241 agatctccca tgcggttaag agacttgagg agggttgact tccctgatcc agatggacca
    18301 atcaaggctg taatttcctt aggttggaaa gatagggaaa cactattcaa agccttcttt
    18361 ttattataat aaacggacag gtctgatacc tgtaaaatcg catctgtcat acggtttcct
    18421 ttctaaccaa agtgaccaga tacatagtca ttggtggact gtagcttggc attttggaaa
    18481 atagttgcag tcttgtcata ctcaatcaaa tcacccaagt aaaagaagcc tgtatagtca
    18541 cttgcacgag cagcctgctg catattatgc gttacaatga tgatggtaaa gtttttcttg
    18601 agctcaaaca tggtctcttc tagttgcatg gtcgcaatcg gatccaaggc tgaggctggc
    18661 tcatccatta agaggatatc tggcttaaca gagatggcac gagcgataca gagacgttgt
    18721 tgctgaccac ctgataaggt caaggctgac ttgtggagat cgtctttaac ctgatcccag
    18781 agggcagcct gacgaaggga ggtttctacg atttcatcta ggacttgctt atccttaact
    18841 ccagcacgtt catgcgcaaa ggtaatatta cggtaaattg acttagcaaa tggattgggg
    18901 cgttgaaaaa ccattccaat gtgtttacgc atttcataaa cgttgatttc tggacggttg
    18961 acatcaattc cacgatagag aatctgccca gttactttag caatatcaat agtatcattc
    19021 atgcgattga gactgcgtaa gtaggtagat ttccccgatc ccgacggacc aatcaaagct
    19081 gtaattttat ttctttcaaa ttgcatatca atccccttaa tggattcatt tttaccatag
    19141 taaacatgga catccttagt agaaagggct actttttctt caggaaaggt aaggatatgc
    19201 ttctcatccc agttatatgt tgacatggct tctcctttag gcagcggtta atttcttgtg
    19261 tagatagctt ccgaacttac gagctccaaa gttaaaaatc aggataaaga tcaggagcac
    19321 agcggcagaa cctgctgata caattgttcc atctggaata gtgccttcac tattgacttt
    19381 ccagatatgg acagccaagg tttctgcttg acggaagata gagatggggc tagtcacact
    19441 gaggatattc cagttagacc agtcaagagc tggcgccgat tgccctgctg tatagatcag
    19501 agctgcagct tcgccaaaga tacgaccaga tgccaagacg acacccgtta caatacctgg
    19561 aagcgcttct ggaataacaa catgaaccac tgtctcccag cgagaaatcc caagagccag
    19621 accagcctca cgttgggtat ggtgaacgtg tttcaaacta tcctctacat tacgcgtcat
    19681 ctgaggcaag ttaaagactg tcaaggccaa ggcacctgaa atgattgaaa atccatactc
    19741 aaactggact acaaagatca agtaaccaaa gagacccacc accactgatg gtaaagagga
    19801 caagatttca atacaagtcc gcacaaagtt ggtaacagga ccttttttag catattcagc
    19861 caagtaaatc ccagctccca tagaaagagg tacagaaata atcaaggtaa tgaccaatag
    19921 gaaaaaggaa ttgtaaagct gaatgccaat cccaccacct gcttgaaaag cagaagacct
    19981 tccagtcaag aaagaccaag agatatgggg caagccccga accaagatat agagaatcaa
    20041 ggaagccaag attgtcacaa tgatgctagc aatcgtatag aggacagctg ttgcaagttt
    20101 atctaatttc ttagcgcgca taatttttct ttcctctttc tttcgtaatc aatttaatca
    20161 cactgttaaa aactaaactc atcaagagca gtaccaaggc cagtgaccag agaacattat
    20221 tatttacagt tcccatgaca gtgttcccaa ttcccatagt taatatagaa gttaaagttg
    20281 cagctggtgt ggtcaaggaa gttgggataa cagctgagtt tccgacaacc atctggatag
    20341 ctagagcctc accaaaggca cgcgccatcc caaagaccac tgcagtgaaa ataccagaac
    20401 gggccgcctt caagatcaca cgccagatag tctgccagcg agtagctccc atagcgaaac
    20461 tggcctcacg ataataacga ggaaccgcac gcaagctatc cgttgtcata aaggttacgg
    20521 tcggcaaaat catgacaaag aggacggaaa tccctgacaa aatcccaaaa ccagtcccac
    20581 caaagacact gcgaacaaag ggaacgacga cttgcaagcc aataaatccg tacactactg
    20641 aaggaatccc aaccaggagt tcaatagctg gttgcaaaat cttcgcccct tttggtgata
    20701 cttcggtcat aaaaactgct gcaccaatag caaagggtgt tgcgataagg gctgagagaa
    20761 tggtaacgat aaaggaaccc aaaatcatag gaagggcacc aaattcttta ctagaaggat
    20821 tccaagttcc tcccaaaaga aagtcaaaga tattcacacc attgacaaag aaggtcgaca
    20881 agcctttttg cgctacgaaa accaaaatca tggccacaag gatgactatc aaagaaagac
    20941 aggcaaaggt caaacctttt cctaatttct ccagacgaga attctttgat ggaagcaaca
    21001 ttttcttagc taattcttct tgattcatta ttgtctccct tccaacattg tcacagttcc
    21061 ggcagcatct ttttcaacct tcatttcctt aatcggaata tacttcaatc ctttgacaat
    21121 cccttcttgg gtctcatccg agagaacaaa attgagaaat tctgcagcca actcattggg
    21181 ctgccccaat gtatacatat gctcataaga ccacaagggc caattattgc tacttatatt
    21241 ttctggactt aagtcatagc cattcaactt catgcttttg accgaatcat ctatataggt
    21301 aagagataaa taagagatag ctcctggact ttttgatacg attgatttta ccgctccatt
    21361 tgaatcctgc tcctgacttt gcatggcaga ctgaccttcc ataatgacag tatcaaaggt
    21421 agcacgagag ccagagccgg ctgcccgatt gataacagag atgggtaagt ccttaccacc
    21481 aacctctttc caattggtta cctcacctat gaagatttga cgaagttgct ctgtcgttag
    21541 gttatcaaca tcaacctcct tattgacaat cagagccaag ccagctaccg cgaccttgtg
    21601 gtcaacaaga gcagaagcat caattccgtc tttttcctca gcaaatacat ctgagtttcc
    21661 tatatcaact gccccagact gaacctggga caagcctgta ccagaacctc ccccttggac
    21721 attgaccgtt tttccaacat ggatcgtgcc aaattcatct gccgctactt caaccaaggg
    21781 ttgcaaggca gttgagccaa cagccgttat ggattctcca cgatcaatcc agctagcaca
    21841 gcctactaaa caagccgtca gccaaaaagc gataagagac agagcaagct tttttctttt
    21901 tttcactgtt tttctcctcg aaaataatta tgaatactgt gaatttttta agtagttctt
    21961 tatgagttga cgcatgaatt cttaccaaat ttctgcgcaa ttgattattt atataatata
    22021 ggctatatta ctctttccta acctcctttt ttcatatgtg gataaaatct cttgtctatc
    22081 ccttccccca ttgtcaccca ttatagtcat ttcgtgtctc tttttcccct ttttaatgca
    22141 agggaaatta ctctcctcag atgataatcc aaaagctaga aaggtatctc aaacctctct
    22201 actctcccag actagtttac aactaaaagg aaaagattct attttatgag aaatctagtt
    22261 tacaagcggt aagaacgcta ataactaaac ttcttgtact ctttgaaaat ctcttcaaac
    22321 cagtgttttg agctatctat ggctagcttc ctagtttgct ctttgatttt catcgagtag
    22381 taaaactaca tgtgatggca atcaagatat caagaatcat cctactaaaa aaatccatac
    22441 tttcactata acatagaata agatatttga ctagcatttt catttgaatc tgaggccttt
    22501 tggaaaataa tttttcaaaa catttccagt aacctttgca aagcccaagc cattgccttt
    22561 aaccaaaact tggtaccaac catttggcag actttctgcc agctgaacgg tttctccagc
    22621 cgcatacttg acaaacgctt cttggccaat ttcaaccgac tgttcgacct gactcggttt
    22681 caaggctaaa ccaagagcga aactgggctc aaagcgtttc ttcttaaaag tgcccagatg
    22741 cagtccattg cgagcaatct tgagcttccc taaatctggc aaaagttctg gcaagagata
    22801 aagctggtct ccaaaagtct gcaagatacc cggtagattg accttcaaat ggttttgggc
    22861 aaattcctgc cacaaggcaa cttgttcacg gctgaggtta ctcttacttg ccttaaattt
    22921 aggagctgga ttgttaccct taaactgtag atgggcaaca aactgaccct ctcccttaaa
    22981 ctgatgaggg tacatccgag ccgtttctgg caggtcaata ccagctacca ttccattgat
    23041 atgctctact ggcaacaagt caaaatcata ctcttccagc aaccaattga caatctcttc
    23101 gttttcctcg ggtgcccagg tacaggtcga ataaaccaga cgaccacctt cagctaacat
    23161 ggtcactgca tcctccagaa tttctctttg caagctagca cattgactcg gataatctaa
    23221 gctccaatag tccatagcat caggctgctt acgaaacatt ccttcaccag agcaaggggc
    23281 atcaagaacg attaagtcaa aatagccttt aaagaccttg accaagcggt cggcagattc
    23341 attggtcacc acgacatttg tcgctccaaa acgctccatg ttttcaacca aaatcttagc
    23401 ccgtttgctt gaaatttcat tggaaacaag tagcccctcc cctgctagat aggctgccag
    23461 ttgagttgat ttgccccccg gtgcagcagc caagtccaag accttcatac caggactggg
    23521 ttgggctact tgagccacca tttgagcagc aggttcttgc gaataaacta aacctgtagc
    23581 atgctcaggc gatttccctg aaaccttccc atagtggccc caaggggttt gagtaatggc
    23641 atcagaaaag gaaagttgct cttcttttaa gggattgacc cgaaaggccg aaaccgcttc
    23701 ctcctcaaaa gaggcaagaa aatctcttgc ctcatctcct agtatttctt tatatttttc
    23761 aacaaatcct tctggaaatt gcatttaagt tcttttcctt tcgtaaatat aggactgaat
    23821 ttcctcctgc atctcaagag gcaccatcat gacaggctgt ctggtttgaa aatcaggagc
    23881 ttcaccaaaa agggtcacaa cccgatagcc cagactttcc cctaaaatac tagctgcggc
    23941 ataatcccat ggttgcagat aagtgagata ggtcaacaaa cgccctgaca aaatcttggc
    24001 aaaactaatg gccgcacttc catagacacg aacaccaaga accgctcggc tcaaatcagc
    24061 cagcccccat tcattggttt ccagcatacc actattccct gcaatgagaa aatctccaag
    24121 tggtttagtt ttaaaaggag ctaggggcct atcatttaga caaactggaa attccccacc
    24181 accgtggtaa caatcccctt tgaccacatc ataaatcaga ccaaactgtc cctgaccatt
    24241 ttcaaaataa gccatcataa cagcaaaatc ttcctgctgg gctacaaaat tattggtacc
    24301 atcaatggga tcaatgaccc aaaccttgcc ctcttgaacc gaggctcgca gacaaccttc
    24361 ttcagcacaa atcttatcct caggataacg ggacaaaatc tcaccaacca agagttcctg
    24421 aacttctttg tccagtctgg tcaccaaatc tgttggagag gacttggttt caacacgcaa
    24481 gtcttcctgc atatggtcaa gaatgtactg acctgctttc ttaacaagct ctttagcaaa
    24541 ttcaaattta ctttccaaga gaaatctttc cttcctcttt ttctttggca gcctgaactg
    24601 ctcggtaaag tgaatagcca ctaaccgttt caaattctcg tcccaaacgt ttctcttcgc
    24661 tcttgcttgg cacgaccgct ttgaatccct tataggagtc cagtaacttt ttagcctcta
    24721 ctttgccttc ataggcagct tcaacatcat taaaaaaaga aagcactgaa gcaagttctt
    24781 cagtgctcca cgacaaatct agtgggtaac tatactgttt gttcattaac taataccagc
    24841 tctcattctt gcttctttta gttcttgctt acgataacta cgagggagaa aagcacgaat
    24901 ctcatcttca ttaaaaccga tttgcatacg cttggcatca ataataattg gacgacgcaa
    24961 aagactagga tactgctcaa tcaaatgaag caattccgat accgaaatac tctctacatc
    25021 aatattcaat ttttgaaaaa tttttgaacg agttgaaatg atgtcatcag taccattttc
    25081 ggtcaaggaa aggatgtgtt gcaattcttt tcttgttaaa ggactggtca taatattgtg
    25141 ttccacaaag ggaaccttat gtttttctaa ccaggcctta gccttacgac atgatgtaca
    25201 gctcggtgat agaaatagtg taatcatgct tttctcttct tatctatact ttgctacttc
    25261 tattatacaa aaaaataagg cgcttgacta gggattttta gaaaaaaagc ctattttttc
    25321 aagaaaaata ggctgtttgc gaacgattga c
//
LOCUS       DAVKDZ010000023        25061 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000023 DAVKDZ010000000
VERSION     DAVKDZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25061
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.023"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            139..468
                     /locus_tag="VWZ30_000957"
     CDS             139..468
                     /locus_tag="VWZ30_000957"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9334720.1"
                     /translation="MIRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVR"
     gene            590..1468
                     /gene="mvk"
                     /locus_tag="VWZ30_000958"
     CDS             590..1468
                     /gene="mvk"
                     /locus_tag="VWZ30_000958"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU9334721.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLSHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            1450..2403
                     /gene="mvaD"
                     /locus_tag="VWZ30_000959"
     CDS             1450..2403
                     /gene="mvaD"
                     /locus_tag="VWZ30_000959"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU9334722.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMAFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEYLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            2390..3397
                     /locus_tag="VWZ30_000960"
     CDS             2390..3397
                     /locus_tag="VWZ30_000960"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU9334723.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKAAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLTSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            3381..4391
                     /locus_tag="VWZ30_000961"
     CDS             3381..4391
                     /locus_tag="VWZ30_000961"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU9334724.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SSQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEANDQMK
                     KA"
     gene            4469..5167
                     /locus_tag="VWZ30_000962"
     CDS             4469..5167
                     /locus_tag="VWZ30_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEU9334725.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAVTILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            5164..6159
                     /locus_tag="VWZ30_000963"
     CDS             5164..6159
                     /locus_tag="VWZ30_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9334726.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQPQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            6173..6805
                     /locus_tag="VWZ30_000964"
     CDS             6173..6805
                     /locus_tag="VWZ30_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9334727.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVSSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            6806..7048
                     /locus_tag="VWZ30_000965"
                     /pseudo
     CDS             6806..7048
                     /locus_tag="VWZ30_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            7030..7284
                     /locus_tag="VWZ30_000966"
     CDS             7030..7284
                     /locus_tag="VWZ30_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU9334728.1"
                     /translation="MAANYLKAMQSYLKETDLPKLEQLVVTKSWWDTVDILDRVVGSL
                     VYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLQLAS"
     gene            7309..7575
                     /locus_tag="VWZ30_000967"
                     /pseudo
     CDS             7309..7575
                     /locus_tag="VWZ30_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            7725..7964
                     /locus_tag="VWZ30_000968"
     CDS             7725..7964
                     /locus_tag="VWZ30_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334729.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            8009..8638
                     /locus_tag="VWZ30_000969"
     CDS             8009..8638
                     /locus_tag="VWZ30_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9334730.1"
                     /translation="MVLSKYYGVADGMNVEGRGSANFIKDNVLITAAHNYYRHDYGKE
                     ADDIYVLPAVSPSQEPFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLG
                     TLGLPTSQKNLTGITVTITGYPSYNFKIYQMYTDKKQVLSDDGMFLDYQVDTLERSSG
                     SAVYDASHRVVGVHTLGDGANQINSAVKLNERNLSFIYSVLKGYSLEGW"
     gene            8937..9329
                     /locus_tag="VWZ30_000970"
     CDS             8937..9329
                     /locus_tag="VWZ30_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9334731.1"
                     /translation="MDSQKSLVSYRVIGISDRVLEILKEYPLLEEGDYIFTNNGSSLM
                     ITCFNYFLKNSFRKSEIKKDDFVLTAHVFRYSHISLLAELEVPITAIMDCVDYTNETK
                     ILSVYTHVTEKMKSNITEKLDKLVLEND"
     gene            9490..10773
                     /locus_tag="VWZ30_000971"
     CDS             9490..10773
                     /locus_tag="VWZ30_000971"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU9334732.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQVEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQSLLSADMLKHDITIKKAVELITSTVTVK"
     gene            complement(10821..13187)
                     /locus_tag="VWZ30_000972"
     CDS             complement(10821..13187)
                     /locus_tag="VWZ30_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEU9334733.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLSKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLIRILEKGKQKSQDLETIQKHITTVEQELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLTQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLINDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     IEEKQQAVSETVTSSASEQSYILTEENWDRIPAMIGITDTDLKEIFGK"
     gene            complement(13315..13929)
                     /gene="lepB"
                     /locus_tag="VWZ30_000973"
     CDS             complement(13315..13929)
                     /gene="lepB"
                     /locus_tag="VWZ30_000973"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU9334734.1"
                     /translation="MNLFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQSFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            complement(13941..14813)
                     /locus_tag="VWZ30_000974"
     CDS             complement(13941..14813)
                     /locus_tag="VWZ30_000974"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU9334735.1"
                     /translation="MASITLTPSKKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKDVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLAVAVSSIIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLNFC
                     AKLHFKNTEKAKNA"
     gene            14900..15202
                     /gene="zapA"
                     /locus_tag="VWZ30_000975"
     CDS             14900..15202
                     /gene="zapA"
                     /locus_tag="VWZ30_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU9334736.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSSDDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            15199..15747
                     /locus_tag="VWZ30_000976"
     CDS             15199..15747
                     /locus_tag="VWZ30_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU9334737.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            15851..18187
                     /locus_tag="VWZ30_000977"
     CDS             15851..18187
                     /locus_tag="VWZ30_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU9334738.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTDKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QNFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRGVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGVFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLASEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDDRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKHVNVVKRTSGRGPQARLDLRGKRYEEAMNELDAFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     regulatory      18351..18439
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            18530..19852
                     /locus_tag="VWZ30_000978"
     CDS             18530..19852
                     /locus_tag="VWZ30_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEU9334739.1"
                     /translation="MLELLKSIDAFVWGPPLLILLLGTGIYLTIRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDQGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPATIALVFTSAFSPLAVVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     gene            20070..20618
                     /locus_tag="VWZ30_000979"
     CDS             20070..20618
                     /locus_tag="VWZ30_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU9334740.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKANNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLT
                     ILCNYANNLANTPINPELQPYA"
     gene            20728..21627
                     /locus_tag="VWZ30_000980"
     CDS             20728..21627
                     /locus_tag="VWZ30_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEU9334741.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIADFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLTE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(21652..22926)
                     /gene="serS"
                     /locus_tag="VWZ30_000981"
     CDS             complement(21652..22926)
                     /gene="serS"
                     /locus_tag="VWZ30_000981"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU9334742.1"
                     /translation="MLDIKRIRTDFETVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQTRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(23140..23511)
                     /locus_tag="VWZ30_000982"
     CDS             complement(23140..23511)
                     /locus_tag="VWZ30_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEU9334743.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNIE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(23514..24878)
                     /locus_tag="VWZ30_000983"
     CDS             complement(23514..24878)
                     /locus_tag="VWZ30_000983"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU9334744.1"
                     /translation="MKVVKFGGSSLASATQLEKVLNIVKSDSDRRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYIHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREIGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRELTPIKEEEILRQLVQKAKVDHAEIERDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
BASE COUNT         7205 a         4837 c         5642 g         7377 t
ORIGIN      
        1 tatattttta catcttgaat tgtcaaatta agtgtataac tttaccgtag catacttcat
       61 aaggcgtttt ccacttaaga aattttcgtg gtcgattatt tagaacctgt attcacaaaa
      121 aaatgttaaa ctagagatat gatacgcaaa gcatatgata ctgatttaaa cgatcaagag
      181 tgggcgaaga ttgaacccta tttctccaaa caccgcacct ataaatggcc gaaacgagta
      241 cttgttaatg agactttgta cgtcactaaa acaggttgtc aatggcgcat gctccctcac
      301 gactttcctc tttacttaat ggtatggagt ttctttcgtc gttctatgac gacaggttgg
      361 ttccaggtca atggcagatg gtactacgct tatagctcag gtgccttggc agtgaatacg
      421 actgtagatg gctattctgt caactataat ggtgaatggg ttcggtaatg aaagaagcga
      481 ttgtgaatgg aaacaatcgc tttttttgtg aaaatataat aaaatagata ggaaagaatt
      541 aatacttgta tgaaaaataa gacgtttttt cgtctagtaa aaggaaaaaa tgacaaaaaa
      601 agttggtgtc ggtcaggcac atagtaagat aattttaata ggggaacatg cggtcgttta
      661 cggttatcct gccatttccc tgcctctttt ggaggtggag gtgacctgta aggtagttcc
      721 tgcagagagt ccttggcgcc tttatgagga ggataccttg tccatggcgg tttatgcctc
      781 actggagtat ttggatatca cagaagcctg cattcgttgt gagattgact cggctatccc
      841 tgagaaacgg gggatgggtt cgtcagcggc tatcagcata gcggccattc gtgcggtatt
      901 tgactactat caggctgatc tgtctcatga tgtactagaa atcttggtca atcgagctga
      961 aatgattgcc catatgaatc ctagtggttt ggatgctaag acctgtctca gtgaccaacc
     1021 tattcgcttt atcaagaacg taggatttac agaacttgag atggatttat ccgcctattt
     1081 ggtgattgcc gatacgggtg tttatggtca tactcgtgaa gccatccaag tggttcaaaa
     1141 taagggcaag gatgccctac cgtttttgca tgccttggga gaattaaccc agcaagcaga
     1201 agttgcaatt tcacaaaaag atgctgaagg actgggacaa atcctcagtc aagcgcattt
     1261 acatttaaaa gaaattggag tcagtagccc tgaggcagac tttttggttg aaacgactct
     1321 tagccatggt gctctgggtg ccaagatgag cggtggtggg ctaggaggtt gtatcatagc
     1381 cttggtaacc aatttgacac acgcacaaga actagcagaa agattagaag agaaaggagc
     1441 tgttcagaca tggatagaga gcctgtaaca gtacgttcct acgcaaatat tgctattatt
     1501 aaatattggg gaaagaaaaa agaaaaagag atggtgcctg ctactagcag tatttctcta
     1561 actttggaaa atatgtatac agagacgacc ttgtcgcctt taccagccaa tgtaacagct
     1621 gacgaatttt acatcaatgg tcagctacaa aatgaggtcg agcatgccaa gatgagtaag
     1681 attattgacc gttatcgtcc agctggtgag ggctttgtcc gtatcgatac tcaaaacaat
     1741 atgcctacgg cagcgggcct gtcctcaagt tctagtggtt tgtccgccct ggtcaaggct
     1801 tgtaatgctt atttcaagct tggattggat agaagtcagt tggcacagga agccaaattt
     1861 gcctcaggct cttcttctcg gagtttttat ggaccactag gagcctggga taaggatagt
     1921 ggagaaattt accctgtaga gacagacttg aaactagcta tgattatgtt ggtgctagag
     1981 gacaagaaaa aaccaatctc tagtcgtgac gggatgaaac tttgtgtgga aacctcgacg
     2041 acttttgacg actgggttcg tcagtctgag aaggactatc aggatatgct gatttatctc
     2101 aaggaaaatg attttgccaa gattggagaa ttaacggaga aaaatgccct ggctatgcat
     2161 gctacgacaa agactgctag tccagccttt tcttatctga cggatgcctc ttatgaggct
     2221 atggcctttg ttcgccagct tcgtgagaaa ggagaggcct gctactttac catggatgct
     2281 ggtcccaatg ttaaggtctt ctgtcaggag aaagacttgg agtatttgtc agaaattttc
     2341 ggtcagcgtt atcgcttgat tgtgtcaaaa acaaaggatt tgagtcaaga tgattgctgt
     2401 taaaacttgc ggaaaactct attgggcagg tgaatatgct attttagagc cagggcagtt
     2461 agctttgata aaggatattc ccatctatat gagggctgag attgcttttt ctgacagcta
     2521 ccgtatctat tcagatatgt ttgatttcgc agtggactta aggcctaatc ctgactacag
     2581 cttgattcaa gaaacgattg ctttgatggg agacttcctc gctgttcgcg gtcagaattt
     2641 aagacctttt tccctaaaaa tctgtggcaa aatggaacga gaagggaaaa agtttggtct
     2701 aggttctagt ggcagcgtcg ttgtcttggt tgtcaaggct ttactggctc tctataatct
     2761 ttcggttgat cagaatctct tgttcaagct gactagcgct gtcttgctca agcgaggaga
     2821 caatggttcc atgggcgacc ttgcctgtat tgtggcagag gatttggttc tctaccagtc
     2881 atttgatcgc cagaaggcgg ctgcttggtt agaagaagaa aacttggcga cagttctgga
     2941 gcgtgattgg ggatttttta tctcacaagt gaaaccaact ttagaatgtg atttcttagt
     3001 gggatggacc aaggaagtgg ctgtatcgag tcacatggtc cagcaaatca agcaaaatat
     3061 caatcaaaat tttttaactt cctcaaaaga aacggtggtt tctttggtcg aagccttgga
     3121 gcaggggaaa gccgaaaaag ttatcgagca agtagaagta gccagcaagc ttttagaagg
     3181 cttgagtaca gatatttaca cgcctttgct tagacagttg aaagaagcca gtcaagattt
     3241 gcaggccgtt gccaagagta gtggtgctgg tggtggtgac tgtggcatcg ccctgagttt
     3301 tgatgcgcaa tcaaccaaaa ccttaaaaaa tcgttgggcc gatctgggga ttgagctctt
     3361 atatcaagaa aggataggac atgacgacaa atcgtaagga cgagcatatc ctctatgccc
     3421 ttgagcagaa aagttcctat aatagctttg atgaggtgga gctgattcat tcttccttgc
     3481 ctctttacaa tctggatgaa atcgatcttt cgacagagtt tgctggtcga aagtgggact
     3541 ttccttttta tatcaatgcc atgactggtg gaagtaataa gggaagagaa atcaatcaaa
     3601 agctggctca ggtggcggaa tcctgtggta ttttatttgt aacgggttct tatagcgcag
     3661 ccctcaaaaa tccaacggat gattcttttt ctgtcaagtc tagtcatccc aatctcctcc
     3721 ttggaaccaa tattggattg gacaagcctg tagagttagg acttcagact gtagaagaga
     3781 tgaatcctgt tctattgcaa gtgcatgtca atgtcatgca ggaattactc atgcccgagg
     3841 gagaaaggaa gtttagaagc tggcaatcgc atctagcaga ttatagcaag caaattcccg
     3901 ttcctattgt cctcaaggaa gtgggctttg gaatggatgc caagacaatc gaaagagcct
     3961 atgaattcgg tgttcgtaca gtggacctat cgggtcgtgg tggcaccagc tttgcctata
     4021 tcgaaaaccg tcgtagtagc cagcgtgatt acctcaatca atggggtcag tctaccatgc
     4081 aggcccttct caatgcccaa gaatggaaag ataaggtcga actcttggtt agtggagggg
     4141 ttcggaatcc gctggatatg attaagtgct tggtttttgg tgctaaggct gtgggattgt
     4201 cacgaaccgt tctggaattg gttgaaacct acacagttga agaagtgatt ggcattgtcc
     4261 aaggctggaa agcagatcta cgcttgatta tgtgttctct taactgtgcc accatagcag
     4321 atctacaaaa agtagactat cttctttatg gaaaattaaa agaagcaaat gatcagatga
     4381 aaaaggcgta accagcgcct ttttttccat cctcagactg aggtgacttt tttgaattgt
     4441 gataaaatag aagggagagg atgaacctat gagaaaattt aaaatctttt tatttatcga
     4501 agcctgtctt ctgacaggag ctctgatttt gatggtatca gagcattttt cgcgttttct
     4561 gctgatacta ttcctctttt tgcttttgat tcgctactac actggtaaag agggaaataa
     4621 tcttctttta gtagcggtaa ccattctctt ctttttcatc gttatgctca atccttttgt
     4681 gattctagct atttttgttg cggttatcta tagcctcttt cttctttacc cgatgatgaa
     4741 ccaggaaaaa gagcagacca atttggtttt tgaagaggtc gtgacggtta agaaggagaa
     4801 aaatcgttgg tttggaaatc ttcatcattt ttcaagctac cagacttgcc aattcgatga
     4861 tatcaatctc tttcgcttca tgggcaagga cactattcat ctggagaggg tcatcttaac
     4921 caatcatgac aatgtcatta tcctcagaaa gatggtagga acgaccaaaa tcatcgtacc
     4981 tgtagatgtg gaagtcagtc tcagcgttaa ctgtctctat ggggatttga cttttttcaa
     5041 ccagcccaag cgagccctcc gcaatgaaca ctatcatcaa gaaacaaaag actatctcaa
     5101 gagtaacaag agtgtcaaga ttttcttgac cactatgatt ggtgatgtgg aggtggttag
     5161 aggatgaaaa aacaagccta tgtcattatt gctctcacct ccttcctatt tgtctttttt
     5221 ttctcccaca gcttgctgga aatacttgat tttgactggt ctatcttttt gcacgatgtc
     5281 gaaaaaacag aaaaatttgt ctttttattg ttggttttca gcatgtccat gacctgtctc
     5341 ttagccctgt tttggcgagg gatcgaagag ctttctctaa gaaaaatgca ggctaatctc
     5401 aagcgtttat tggcagggca agaagtagtt caggttgtag atccagattt ggatgccagt
     5461 ttcaagtcct tatcaggtaa acttaacctt ttgacagagg ctcttcaaaa agctgaaaat
     5521 catagccttg ctcaggaaga ggaaatcatc gagaaggaac ggaagcgaat tgctcgggat
     5581 ttgcacgata cagtcagtca ggagttgttt gcggcccaca tgattttatc gggtatcagt
     5641 cagcaggctt tgaaattgga tagagaaaag atgcagcccc agttgcagag tgtcacagct
     5701 attttagaaa cagcccagaa ggatttgcgg gttttgctct tgcatttgcg accagttgaa
     5761 ctggagcaga agagcttgat agaagggatt caaattctct taaaagagct tgaggacaag
     5821 agtgatctta gggttagtct caagcagaat atgacgaaat tgcctaagaa aatcgaggag
     5881 catatcttcc gtatcctgca agagttgatt agcaatacgc tccgccatgc ccaggcatct
     5941 tgcctagatg tctacctcta tcagacagat gttgaattgc aactgaaggt ggtggacaat
     6001 gggattggtt tccagttagg gagcttagac gacttgagtt atggactgcg aaatatcaag
     6061 gagcgggttg aagatatggc tggaacagtt caactcttga cagctcccaa gcaagggctg
     6121 gcggttgata tccgtattcc cctgttagat aaggaatgat aaaggagtaa agatgaaaat
     6181 tttactagta gatgaccatg aaatggtccg attgggcttg aaaagctact ttgacctcca
     6241 agatgatgta gaagttgtgg gtgaggcgtc caacgggtct caagggattg acttggcctt
     6301 ggaactgcgt ccagatgtca ttgtcatgga tattgtcatg cctgagatga atgggattga
     6361 cgcgacctta gcaatcctta aagaatggcc tgaagccaag attttgattg tgagctctta
     6421 tttggacaat gaaaaaatca tgccagtctt agatgctggt gccaaaggct atatgctcaa
     6481 gacttctagt gcagatgaat tgcttcatgc cgtcagtaag gtagctgctg gcgagctggc
     6541 cattgagcaa gaggttagca agaaggttga ataccaccgc aatcatatgg aacttcatga
     6601 agaattgact gcgcgtgagc gagatgttct ccaactcatc gccaagggct acgaaaatca
     6661 gcgcatcgca gatgacctct ttatctctct caagacggtc aagacccacg tgtccaatat
     6721 tcttgccaaa cttgaagtca gcgatcgtac ccaggcggct gtctatgcct ttcagcacca
     6781 tttggtgggg caagaggagt tttagatgag tttagcagat ttacttgagg agctagaagc
     6841 agcaaaagac tctaaaaaag caaggcccat gtaagcctat atgcgccatc aattttcttt
     6901 tctaggtatt gcggtgcctg aaagaaataa actctataaa aatatttacc agaagcgaaa
     6961 aaaacaaaga ttatagattg ggattttgca gacacttgct gggaaaagga gcctagaaaa
     7021 taccaatatg tggctgctaa ctatttgaaa gcaatgcagt cttatctaaa ggaaaccgat
     7081 ttgcctaagc tagagcagtt agtcgtgaca aaatcctggt gggacacggt agatatccta
     7141 gatcgagtag tagggagttt ggtgtatgaa caccctgaac tagaagaaat aatcttaata
     7201 ctcttcgaaa atctcttcaa accacgtcag ctctatctgc aacctcaaaa cagtgttttg
     7261 agcaacctgc agctagcttc ctagtttgct ctttgatttt cattgagtat aaaatggagc
     7321 ctatcagata atatctggct gagacgtgtc gctattgacc accagttgtt aagaaaagag
     7381 aagaccaata ctcaattaat ggaaaagatt ctacttcata acttgaacca aacagaattt
     7441 tttatcaata aagccattgg ctggactctg cgagactact ccaaaacgaa tcccacttgg
     7501 gtaacatgct ttattgagaa aaacaaggaa agaatggctg aacttagtat caaagaagca
     7561 agtaagtacc tctagcgtga ttgaaaagcg cagtcattaa ttgaaaagtt tacctgttta
     7621 tgtgataatg gagggtattt aaagaaggtg gtagatttct tgattctata gtagattaag
     7681 aaatttagaa tagtacgcta taattacatt aggaggatac aataatgaag aaaactgttt
     7741 ataaaaaatt gggtatttca attattgcga gtactttatt ggctagccag ttatcgacag
     7801 tatctgcttt gagtgttatt tctagtacgg gtgaagaata tgaggtaagt gagacactag
     7861 aaaaaggtcc aggatctaat gattcttcat tatctgagat ttcaccaaca tatggttcat
     7921 actaccaaaa gcaatcagaa gtattatcgg taatgatgat ttgattaaaa ttgataatac
     7981 tttacagtat ccgtattcga cttcagctat ggttttatct aagtattatg gagtagccga
     8041 tggaatgaat gtagaaggga ggggaagtgc gaattttatt aaagataatg tgttaattac
     8101 agcggctcac aactactaca gacatgacta tgggaaagaa gcggatgata tttatgttct
     8161 tccggctgtt agtccaagtc aagaaccatt tggaaagatc aaagtaaagg aagttcgtta
     8221 tttgaaggaa tttagaaatt taaattctaa ggatgcaagg gaatatgact tggctttatt
     8281 aattctagaa gagcccattg gtgcaaaatt agggactttg ggtcttccta ctagtcaaaa
     8341 aaatttgaca ggaataactg tgactatcac aggatatcca tcatataatt ttaaaattta
     8401 tcaaatgtat acagataaaa aacaagtttt aagtgatgat ggcatgttct tggattacca
     8461 agttgatact ttagagaggt ctagtggatc tgcagtttat gatgctagtc accgcgtagt
     8521 aggagtgcat actttaggag atggagctaa tcaaattaac agtgcagtta aattaaatga
     8581 acgaaatttg tcatttattt attcggttct taaaggttac tctcttgaag gatggtgaat
     8641 gggttaggta atgaaggcta attgtaaact gtgatggata cttaactttg tataataggt
     8701 ggataaaagt ctttaaaata ggaaaaaacg catattatta ggtgtttaaa aaccttgata
     8761 atatgcgttt tataatggaa agatttcttc aatttcgtct tgtatagtag attgaaacta
     8821 taatagtaca cctctacttc taaaacattg ttagaaattg atttgactgt cctgatcgat
     8881 ttgtcctgtt cttatttcgt tttactataa tgtaatcgtc caataacgaa gtgtttttgg
     8941 atagtcagaa aagtttagtt tcatatagag taattggtat tagtgataga gtgttagaaa
     9001 ttttaaaaga atatcctctt ttagaagaag gtgattatat atttacaaat aatggttctt
     9061 ccttaatgat aacttgtttc aattactttt tgaaaaacag ttttaggaaa agtgagatta
     9121 agaaagatga ttttgtattg actgctcatg tttttagata ttcgcatata agtttgcttg
     9181 ctgaattgga agtacctata acagcgataa tggattgtgt agattatact aatgaaacaa
     9241 agattttaag tgtttatact catgttactg agaagatgaa gtcaaatata actgaaaaat
     9301 tagataaatt agttttggaa aatgactaac caaaagatat ccaaagtagt ctaaaattgt
     9361 ctatacttta tgagtgtttt agttaggaaa aaggcttgtt gtctataatt ggctgcatta
     9421 gtctagattt tatttataga aaatgttata atagactgta tttaaaaaat tttaaggaga
     9481 aatgacagaa tgtctgtatc atttgaaaac aaagaaacaa accgtggtgt cttgactttc
     9541 actatctctc aagaccaaat caaaccagaa ttggaccgtg tcttcaagtc agtgaagaaa
     9601 tctcttaatg ttccaggttt ccgtaaaggt caccttccac gtcctatctt cgacaaaaaa
     9661 tttggtgaag aatcacttta ccaagacgtt atgaacgctc ttttgccaaa cgcttatgaa
     9721 gcagctgtaa aagaagctgg tcttgaagtg gttgcccaac caaaaattga cgtaacttca
     9781 atggaaaaag gtcaagactg ggttatcact gctgaagtcg ttacaaaacc tgaagtaaaa
     9841 ttgggtgact acaaaaacct tgaagtatca gttgatgtag aaaaagaagt aactgacgct
     9901 gatgttgaag agcgtatcga acgcgaacgc aacaacctgg ctgaattggt tatcaaggaa
     9961 gctgctgctg aaaacggcga cactgttgtg atcgacttcg ttggttctat cgacggtgtt
    10021 gaatttgacg gtggaaaagg tgaaaacttc tcacttggac ttggttcagg tcaattcatc
    10081 cctggtttcg aagaccaatt ggtaggtcac tcagctggcg aaaccgttga tgttatcgta
    10141 acattcccag aagattacca agtagaagac cttgcaggta aagaagctaa attcgtgaca
    10201 actatccacg aagtaaaagc taaagaagtt ccagctcttg acgatgaact tgcaaaagac
    10261 attgatgaag aagttgaaac acttgctgac ttgaaagaaa aataccgcaa agaattggct
    10321 gctgctaaag aagaagctta caaagatgca gttgaaggtg cagcaattga tacagctgta
    10381 gaaaacgctg aaatcgtaga acttccagaa gaaatgatcc acgaagaagt tcaccgttca
    10441 gtaaatgaat tccttgggaa cttgcaacgt caagggatca accctgacat gtacttccaa
    10501 atcactggaa ctactcaaga agaccttcac aaccaatacc aagcagaagc tgagtcacgt
    10561 actaagacta accttgttat cgaagcagtt gccaaagctg aaggatttga tgcttcagaa
    10621 gaagaaatcc aaaaagaagt tgagcaattg gcagcagact acaacatgga agttgctcaa
    10681 gtacaaagct tgctttcagc tgacatgttg aaacatgata tcacaatcaa aaaagctgtt
    10741 gaattgatta caagcacagt aacagtaaaa taatcttaat aaacagaaaa cccacctgaa
    10801 ttggtggatt ttctgatgca ctattttcca aaaatctctt tgaggtctgt gtctgtaatc
    10861 ccaatcatgg ctgggatgcg gtcccagttt tcttcggtta ggatgtagga ttgttcagag
    10921 gcacttgatg tgactgtttc agagacagct tgttgctttt cttcaatatt ctccagtaga
    10981 tcactgaagc gttcaatcag ataggttttt cgggcagttc cgatgtgttg ggtagcatag
    11041 tcgaaggctt gtaattcgcc tagtaagatg agtttgctct tggcacgtgt aatggctgtg
    11101 tagatgagat ttcgctccag catacgtcgg ctagcactag taatcggtag gatgacaact
    11161 gggaactcac ttccctgaga tttatgaata ctcatggcat aggccaagcg aatcttgtac
    11221 cattcgttac gggggtaaga gacttcatta ccatcaaaat caatgacaat ctcgtcttgt
    11281 ttcgattcgg tgtatttacc aggaatcagg tctgtgatag ctcctaaatc cccattaaag
    11341 acattgattt cagcatcgtt aatcaaatga atgaccttgt ctcccttacg atagtggcac
    11401 tgaggagctt caaaactgag ttgatctttt tgtgggggat tgagcaggtc ttgcgtgagt
    11461 tgattgatag catcaatccc tgccgtccct cggtacatag gagccagaac ttggatatca
    11521 cgggcaggga taccatttct gagggcggca cctaagattt tttcaatggt ggcaggaata
    11581 tggccactag caatttcaaa gtaggaacgg tcagcttttt tttgggtgaa atcagctggc
    11641 aagatgccct gtcgaatctg actagctagg gtgacgatgg ttgattcttc gctttgtcga
    11701 taaatttttt ccaagcgagt ctgaggaatc aagggaatat gaagcagatc cgctagaacc
    11761 tgtccagggc tgacagaagg caactggtcg ctgtctccca cgatgaggat cttactgtta
    11821 gaagagatgt tggagaagag ttgattggcc agccaagtat ccaccataga gaattcatcc
    11881 acgatgataa agtcagcatc taggtaatct tccagatgac tggtatcatc gtcacctgtc
    11941 attcccaagt ggcggtgtat ggtcgcgcta ggcaaacctg tcaattcatt catgcgacga
    12001 gcagctcgac cagtcggagc agcaagaaga atgggcagat tgcttttctt cctgaagtca
    12061 agtccttcta aaagggcata aacagcgatg attccattga taacagttgt cttaccagta
    12121 ccaggcccac ctgtcaggat aaagaccttg ttctggatag cgtcacaaat agcttgtttt
    12181 tgaatgttat catactcaat ccccagttct tgctcgacag tagtgatatg cttttgaata
    12241 gtttctaaat cttggctctt ctgttttcct ttttcaagga tacgaatcaa gtgactgcgg
    12301 ataccttcct cagcgaaaaa gaggctgttg tcaaagatct tggtatcaat ctgctgaacc
    12361 ttgtcttctt cgatcaggta ggagagctct tgggcaactt ggctggggtc tagttccacg
    12421 ggacgggaag actcaaggag agtaagggtt tgttccagca aatcccgtgc ttcaacataa
    12481 gtgtctcctg tttccataca ggcctgaaaa agactgtgaa ctagaccggc gcggaagcgt
    12541 tcaggagcct gactttcgat gcctagttcc tcagctagtt ggtcagcaat ggtaaagccc
    12601 aaacccttga tatcctcaac cagctggtag ggataatttt caaccacatc aagggtttct
    12661 tccttgtaaa agtcttgaat ctgaaaggct agtttgttgg gaatgccgta gttggctagt
    12721 ttggacaaaa ccatctccgt tccgtagttg agacggagag tggagacgaa agcctcgcga
    12781 tttttggcag agagtcctgc gatgccttct aacttttctg ggtgttgcaa aatttcgtca
    12841 atggtatttt cgccataggt atctacgatt ttctgagctg tcttgagacc aatccccttg
    12901 aaatggctac ttgaaaagta cttgaccaag cccttactag ttggttttgc gcgatcataa
    12961 cgactaattt gcagttgttc tccatacttg gagtgctgga caatttgccc ccaaaaagta
    13021 tagtcttcgc cctcaattac atcagccatg gttcctgtga caatgatttc aaaatcatca
    13081 aaatcctctg cgtccgtatc gtcgatttct aggaggagga tgcgataaaa attgctggga
    13141 ttttcaaaaa taatccgttc aatggttcct gaaaaataaa cttccataaa attcctttgc
    13201 atgaataggt gagagttgag gtgtttctgt tctggtaagt ttagatagtt accaatcatt
    13261 ttctcacgat agaagaagag gctgagattg gtgattctcg gcctcttagg tctcttaaaa
    13321 tgttccgata cgggtgattg gccagaagcg gaatttagct tcccctgtga tatcttttgc
    13381 tttgaaggta cctacgtggc ggctgtcgct cgaaaccaag cggtcgtctc caaggagaag
    13441 gtattctcct tctggaactg taaagctaaa gttggtattg tagttgacat caactgtgaa
    13501 ggattgagct ttttgagcga tacttctaaa gaaagttcct ttatttcctt caaatccctt
    13561 gcctgagtag gtgctttgga gtttgtcgtc cttgaagcgt ttgatatagt ctgctagata
    13621 aggttcgtcc gtttctttgt cattgatgta gagtttatca ttttcgtaac ggatggtatc
    13681 gccaggcatt ccaatcacgc gcttgacgat gtccttattg ccatcttcct catgggccac
    13741 cacgatatca aaacggtcaa tagggaggtg cttaacaaca aagaggattt caccatccgc
    13801 tagggtcgga tccatggaat gtccttctac gcgaacattg ctccaaaaaa agatacggct
    13861 caaagctagt aatgatagaa tcaggaggaa taatccccac tcttttaaga aatttttaaa
    13921 taaattcata acttaccttt ctaagcgttt ttcgcttttt cagtattttt aaagtgtagc
    13981 ttagcgcaga agttgagtcc ctgcatacca taagcttgca aaatctggct agccaccttg
    14041 tcagaagccg ttccagctcc acttggaagc tgataaccca gttctcgtcc caaattttca
    14101 agattttcca gaaagagatc acgcgcaatg atggaagaaa ctgcaacagc caagtatttg
    14161 ccctcagcct tttcttctaa gctgatagga ttgctgaaac gattggcctc ttgtgccaag
    14221 tacttgtcat aatttttagc actggtaaag gcatcaatca caattttctc aggctgaaca
    14281 tctttttgaa ggaggagata gatagcctga ttatggaggg caaccttaac cgaaacagca
    14341 ttgtagcggt ctccgatgac ctcgttgtac ttgctgggtg agagaaggag tgcctggtgc
    14401 tggatttttt ccttgagaat aggagcaatc tgacggatct tttggtcggt cagagtctta
    14461 gaatccccca caccgagttt tcgtaaaaag tcgtgctggt caggtgtgac aaaggcagcc
    14521 acaactgcaa gcccaccaaa gtaggaacca tttcccacct catctgtccc aattaaagga
    14581 agattttgtc cgctggtttg ctctacagct tgatagccaa agaaactggc gtatttttca
    14641 gccccttcac cctgaagcaa gatttttcca gaagtataga tagaaaccgt tgcttgaggt
    14701 agtttcaaaa agtagcggat atagggattc ttgctaggag ccagactggt ttgatagtgt
    14761 tcaagaaaag cctgaatatc ctttttgctt ggtgtgagtg tgatacttgc catagtttct
    14821 attgtaccat aaaagcagta aaatttgtaa aaactgacaa aattagcgaa ttttggtata
    14881 atatcgtgag gtgaatttta tggcaaatct aaatcgattc aaatttacat tcgggaaaaa
    14941 atcgttaacc ttgacaagcg aacatgataa cctttttatg gaggaaatcg ctaaggttgc
    15001 gacagaaaaa taccaagcaa ttaaagaaca aatgcctagc tcagatgatg aaacaatcgc
    15061 tcttttgttg gcagtcaact gtttatcaac tcagctcagc cgtgagattg aatttgacga
    15121 taaggagcaa gagctagaag aactccgtca caagcttgtg acttgtaagc aagaacagag
    15181 caagattgag gattccttat gatttcattc cttcttctat tggtcttggt ttggggattt
    15241 tatatcggct atcggagagg cctgctctta caggtttatt acctgatttc agccatggca
    15301 tcggctttta tggctggcca gttttataag gggcttggag agcaattcca tttattgctc
    15361 ccttatgcaa attcgcagga aggtcagggg actttctttt tcccatcgga tcaactcttt
    15421 cagctggata aggtctttta tgcaggtatc ggctacttgc ttgtatttgg gattgtctat
    15481 agcattggtc gtttgcttgg tcttctctta cacttgattc ctagcaaaaa actgggtggt
    15541 aagttgttcc aagtttcagc aggtatcttg tccatgttgg tgaccttatt tgtcttgcaa
    15601 atggccttga caatcttggc gaccatcccc atggcagtta tacaaaatcc tcttgaaaag
    15661 agtatcgtcg caaaacacat catccagagc ataccgataa caaccagttg gctcaaacaa
    15721 atctgggtga caaatttaat cggataaaaa gggcaggagt tttcctagcc ctttgtttac
    15781 agatttgact cgaatctatc agaatgtaaa aagctaccac acctagacat tcaaagacaa
    15841 ggaaataaag atgaataaga aaatattaga aacattagag ttcgataagg tcaaggcctt
    15901 gtttgagcct catttgttga ccgagcaggg cttggagcaa ttgagacagc tggctccgac
    15961 tgacaaagca gataaaatca aacaggcttt tgctgagatg aaggaaatgc aggctctttt
    16021 cgtcgagcaa ccgcatttta ctattctctc aactaaggaa attgcaggag tctgcaagag
    16081 gttggagatg ggagcggatc tcaatatcga ggagttccta ctcttgaaac gcgtgcttct
    16141 tgccagccga gaacttcaaa atttttacgc caatctggaa aatgtcagct tggaagaatt
    16201 agccctttgg tttgagaaat tacatgattt tccgcaatta caaggaaatc ttcaggcctt
    16261 taatgatgca ggcttcattg aaaattttgc cagtgaagaa ttggcgcgaa tccgtcgaaa
    16321 aatacatgat agcgagagtc aggtacgcgg tgttttacaa gacttgctca agcaaaaagc
    16381 gcaaatgttg acggaaggaa ttgttgctag cagaaatggc cgtcaggttt taccagtcaa
    16441 aaacacctac cgcaataaga ttgcaggtgt cgttcatgat atttctgcta gtggaaacac
    16501 cgtctatatc gaaccccgtg aggtagtcaa actgagcgaa gaaattgcta gtctgcgagc
    16561 agatgagcgc tatgaaatgc ttcgcattct ccaagaaatt tctgagcgtg tccgccctca
    16621 tgcggctgag attgctaatg acgcttggat tatcggtcat ctggacttga ttcgtgccaa
    16681 ggttcgattt atccaagaaa gacaagcagt cgtgcctcag ctgtcagaaa atcaagagat
    16741 tcaactgctc catgtctgcc atcctttggt caaaaatgcc gtcgcaaatg atgtccattt
    16801 tggtcaagat ttaacagcta ttgtcattac aggtcccaat acaggtggga agaccatcat
    16861 gctcaaaact ctgggcttga cacaggtcat ggcccagtca ggattgccga ttttagcaga
    16921 caagggaagt cgtgttggtg tttttgaaga aatctttgct gatattggag atgagcagtc
    16981 tattgagcag agcttgtcta ccttctctag ccatatgacc aatatcgtgg atattcttgg
    17041 caaggtcaac caacattcac tcttactttt ggatgagttg ggggctggta ctgatcccca
    17101 agagggagca gcccttgcca tggctattct ggaggacctt cgcctgcgtc aaatcaagac
    17161 catggcgacg acccactatc cagaactcaa ggcctacggt attgagacag cctttgtgca
    17221 aaatgccagt atggagtttg atactgcaac tcttcgcccg acctatcgct ttatgcaggg
    17281 tgttcctggc cgaagtaatg cctttgaaat tgccaaacgt ctaggcctat ctgaagttat
    17341 cgtaggagat gccagtcagc agatcgatca ggacaatgac gtcaatcgta tcattgagca
    17401 actagaagag cagacgctgg aaagccgcaa acgcttggac aatatccgtg aggtggagca
    17461 agaaaatctc aagatgaacc gtgcgctaaa aaaactctac aacgagctta atcgtgaaaa
    17521 ggaaaccgag cttaacaagg cgcgtgaaca ggctgctgag attgtggata tggccctaag
    17581 tgaaagtgac cagattctca aaaatctcca cagtaaatcc caactcaagc cccacgaaat
    17641 cattgaagcc aaggccaagt tgaaaaaatt ggcttctgaa aaagtggact tgtctaaaaa
    17701 taaggtcctt caaaaggcca aaaaaaaacg agctccaaag gtgggagatg atatcgtggt
    17761 tctcagttat ggtcagcgtg gtaccttgac tagtcagctc aaggacgacc gctgggaagc
    17821 ccaagttggc ttgattaaga tgaccttgga agagaaagag tttgaccttg ttcaagccca
    17881 gcaagaaaaa ccagtcaaga agaaacacgt caatgttgtg aaacgaactt ctgggcgagg
    17941 acctcaagct agactggatc ttcgaggcaa gcgctatgaa gaagccatga atgagctaga
    18001 tgccttcatc gaccaagcct tgcttaacaa tatggctcaa gttgatatca tccatggtat
    18061 cggaacagga gtcatccgtg aaggagttac caaatacttg caaagaaata aacatgtcaa
    18121 gagtttcggc tatgccccac aaaatgctgg aggcagtggt gcgactattg tcacttttaa
    18181 aggatagcag tattctggac tttataaagt aaaaactgtt gaactaattt ttactaataa
    18241 acacattgac aaaagccaac attttttgta aaattagaat caattaaata ccaacaccga
    18301 atgaagttta atagaagtgg ggaatcgttt gattttccat gactgtaaat ggacggaact
    18361 ctggagagac cgtaaaggca ccgaaggggc aaggcaggca actgctcaaa ctctcaggta
    18421 aaaggacaga gctaggatag acggcttttt agcatttatc taagcattcc agagtacatg
    18481 tatcttgcat gtgctctttc ttttggggtt gaaacgatag gagaaggaaa tgttagaatt
    18541 gcttaaatca atcgatgctt ttgtttgggg accgcccctc ttgattttat tgctcggaac
    18601 agggatttac ctaactattc ggctaggact cttgcaggtt ttgcgtctgc ccaaggcctt
    18661 tcagcttatt tttatccagg ataagggaca tggtgatgta tccagttttg cagctctgtg
    18721 tacagccttg gcatcaactg ttggaacagg aaatatcata ggagttgcga cggctatcaa
    18781 ggttggtgga ccaggagctc tattttggat gtggatggcg gctttctttg gaatggctac
    18841 caagtatgcg gaaggactct tggccatcaa ataccgcacc aaggacgacc aaggtgcagt
    18901 agcgggaggt cccatgcact atatccttct agggatggga gaaaagtggc gaccacttgc
    18961 tgttttgttt gcagtagcag gagtattggt tgctctcttg ggaatcggaa ccttcaccca
    19021 agtcaactcg attacagaat ctatccaaaa tacaacgacg atttcgccag ccatcacagc
    19081 tctcgtcttg tctgtctttg tagcgattgc agtctttggt ggactcaagt ctatttctaa
    19141 ggtttcaact actgttgttc cttttatggc catcatttat atcttaggaa ctcttacagt
    19201 tattttcttt aatatcggaa aaatccctgc cacaatcgct ttagtcttta cctcagcttt
    19261 tagtcccctt gctgtggtag gtggatttgc tggtgctagc gttcggatgg ctattcaaaa
    19321 tggtgtggcg cgtggtgtgt tctcaaacga atctggtcta ggttctgctc ctattgcagc
    19381 tgcagctgcc aagacaaatg aaccagtaga gcaaggtttg atttccatga caggaacctt
    19441 tattgatacc ctcatcattt gtactctaac tggtttgacc atcttggtaa ctggagtttg
    19501 gagtggtgac ttgaatgggg ttgccttgac tcagtcagct ttctcaacag tcttttcaca
    19561 ctttgggccg gccctcttga ccatcttcct tgtacttttt gcctttacaa cgattctagg
    19621 ttggaactat tacggagaac gctgttttga gttcctcttt ggggttcgct ttatctggct
    19681 ctaccgtgtg gtttttgtgc tcatggtctt gttaggagga tttatcgagt tggatatggt
    19741 ctggattatc gcagatatcg ttaacgcctt gatggctctg ccaaacttga ttgccctctt
    19801 ggtcttgtcg ccagtcgtta ttgctgaaac taaaaagtat tttgacaaat aatggaatca
    19861 cacagctcgt gtgatttttt tgcctataaa taatttttat cagcctgata tataatatat
    19921 attatacaag tgattggaag tgtgataaac taatataaca atgaaaatct tatgagagaa
    19981 taaagcttta tcttagataa acttaggaat caggatttta taagattttc caataatatt
    20041 agtgtcaata ataagaaaag aggtatctta tgacaacatt tactatccat acagtagagt
    20101 cagcaccagc agaagtgaaa gaaattcttg aaacagtaga aaaagccaac aatggctata
    20161 ttcccaacct aatcggtctc ttggccaatg ccccgactgt tttagaagcc taccaaattg
    20221 tctcatctat ccaccgtcgc aacagtctga cacccgttga gcgtgaagtg gtgcaaatca
    20281 cggcagccgt gaccaatggt tgtgccttct gtgtcgcagg tcacacagcc ttttccatca
    20341 aacaaatcca gatgaatgat gacttgattc aagctcttcg caatcgtact ccaattgaaa
    20401 cagatcctaa attggataca ctagctaagt ttaccttggc agttatcaat accaagggtc
    20461 gtgtaggaga tgaagccttg tctgagtttt tagaagctgg ctacactcaa caaaatgcct
    20521 tggatgtggt ttttggtgtc agcctaacaa tcctctgtaa ctatgccaac aacttagcta
    20581 atacaccaat taatccagaa ttgcaacctt atgcctaatt catatctgag taaaatgaag
    20641 tctgaaataa gcggactgcg tttttttaaa tcctcattta agcgctttta tgttatactg
    20701 aaactaacat gattattgga ggttaggatg aaaaaactcc ccttagtatt ttcaggttgt
    20761 ttgctaggtt tggcaggagc tggaaatctt attttagata cgttgccggt tctatcccat
    20821 ctttttagtc tgactggttt ggttttatgg atttactttc taattctgca tctctttaat
    20881 tggaaagaaa ccaagcaaga attgaccaag ccccctcttt tgtcaggaat ggcaaccttt
    20941 cctatggctg ggatgatttt atcgacctat gtctttcgcg tattctctta tcttcctttg
    21001 gtagcacaag ggatttggtg gttttcattt ctcttggatt tgaccttgat tgctgacttt
    21061 accatcaagt ttgcttgtcc agggcggagg gttcatgcca ctccaagctg gacggttctc
    21121 tatgtgggga tagcagtggc tgccttgacc tatcctctgg taggtattat cgaaattgcc
    21181 tatgcgacct tgagttttgg ttttctcttg accttctatc tctatcccct tatttatagc
    21241 gatttaaaga aacatccact cccactagcc ttgcttggac aagaaggaat ctactgtgct
    21301 cctttctctc tactcttggc ttctctagtt cgagtaggag gaaccagcct gccgacttgg
    21361 gtcttgattg tcatgatttt ggcttctcaa tccttctttt tctttgtttt aactcgtctg
    21421 cccaacattt taaaacaagg ttttcaacca gccttctcag ccctcacctt cccaaccatt
    21481 atcacagcga cctcgctcaa gatggctcag ggaattttga aacttccatt tctggattac
    21541 ctggtattga ctgaaaccat tatatgccta actattttat tctttgtact aggtgcttat
    21601 ctgatttggt tacgaaaaaa ggtctagcta gaaatagcta aaccttattt tttatggttt
    21661 gatgacttca gctccaccca tgtatggacg aagtgcttct gggatggtca cagaaccatc
    21721 ttcattttgg taattttcaa gaattgcagc cactgtacgt ccaactgcaa gtccagaacc
    21781 gttcaaggta tgaaggagtt tcaccttgcc atctgcttca tcacggtaac gggtttgggc
    21841 acgacgggct tggaaatctt ctgtgtttga acagcttgag atttcacggt aattgttttg
    21901 tgctggaatc cacacttcca agtcgtaagt cttcgcagct gagaagccca tatctccagt
    21961 agagagagca acgacacggt atggaaggtt gagtttttga agaatgtttt cagcgttggc
    22021 tgtcattttt tccaattctt cgtaagattc ttctggtttg gcaaatttga ccatttcaac
    22081 cttgtggaat tggtgcaaac ggatcaagcc acgcgtatca cgaccggcag aaccagcctc
    22141 agaacggaat gacggactca tagcagtgaa gtagattgga agatctttgc cgtctaagat
    22201 ttcatcacgg tagtagtttg tcagaggaac ttcagcagtt ggaatcaaga caaagttggt
    22261 atcgctgagt tcaaaagtat cttccttaaa ttttggatac tgaccagtac caaacataga
    22321 atcatggttg actatgtaag gtgtgatgac ttcagtatag ccttcttttc catgttcatc
    22381 caacataaag ttgtagatag cacgttccaa acgagcaccg aggcctttat agaagaggaa
    22441 gcgagcgcct gttaccttac caccgcgttc ccagtcaagg ataccaaggt cttcaccgag
    22501 atcccagtga gctttaggtt cgaagtcaaa ctcgcgtgga gtaccccaac ggcgaacttc
    22561 cacattgtcg tcttcgtcag ccccaacagg aacgctgtca gctgggatat ttggaagagt
    22621 cgttgtaaat tctgtcaatt tagcatcgat ttctgccaat tcagcatcca aggctttaac
    22681 ctcagcagat agattttgca tggcagcaat cttgtcatct gtattttcct tgttgcgctt
    22741 agcttgggca atctcagcag aaactgtgtt acgttctgct ttgagagttt caaccttgac
    22801 caagatgtta cgacgtttag catcgatttc tttcatttca ttcaagacag cagcatctac
    22861 accacgtgta gctaattttt ctgcgacagt ttcaaaatct gtacgaatac gtttgatatc
    22921 taacataaga actcctttat gaaaaaagca cgcctgacaa aacgttggag tggcagggcc
    22981 acggttccat ccaacttcac aggtgtgcac ttgattgtgt atgtaattgt tactaacggt
    23041 agaatttcac ctatccctcc tatctgctcg cagtacccgc agactttctg aaagaagaag
    23101 ataacctact tatccgttgc tatgattata ctaaagtttc tacttttttg caaatagatt
    23161 tttaaatttt tggctaattg tctgaatcag ggtcggaagt ttgacgacct tgtcattgcc
    23221 tagtttttcg cgtgcaattt tgagaatggc acctgagtct tttgaagcaa agaggaattt
    23281 tcctttgtct gtaaagactt cgaagtggcg gctgattttg cgtccagtga cattggctcc
    23341 aatctgattg atatggctcc aaggaatctg gataaattgt tcgatattga catctgggta
    23401 aaattccaaa gcctgatctc cgacaaggaa tttcccaact ttcccagcga tagagaggta
    23461 ggaagtgcct gtcgtactga ggagtactgt tttgttaagt gattgagcca tgcttagtct
    23521 tccttacttt ctccaaaaaa ggcattgtag agggctttaa ttgctgcttt ctcttggtct
    23581 ttattgacaa caaacataat agaaacttca ctagaacctt gagacatcat ctggatgttg
    23641 attttgtttt cagatagagc gcgtgtcgca gtagcagtca ctccgatatg gctcttcatt
    23701 ttttcaccaa caatcataat gatagaaagg tcgcgttcga tttctgcatg atctacttta
    23761 gccttttgaa ccaactgacg caggatttct tcttccttga tgggagttag ttcgcgagaa
    23821 cggagaatga tagaaagatc gtcgatacct gttggcatat gttcccaacc gatgttaagt
    23881 tcttccagga tttgtagaac cttgcggcca aatccaatct cacggttcat gaggtatttc
    23941 gacatgttaa tgctgacaaa gcctgagtca ccagcaattc ccacaactgg aaattcatca
    24001 ttactgtgtt ttagaacgat acgggtacct ggatggtcag ggttgttggt attcttgata
    24061 accagaggaa tttttccacg gtaggcagga agaagagcct cgtcatgaag gactgagaag
    24121 cctgcatagg ccaactcgcg catttcacgg taggtcaact caggaatcga gtgtggttgg
    24181 tggataatac cagggtgggc tgcaaagata ccatcaacgt ccgtaaagtt ttcatagagg
    24241 tcagctttga caccagcagc aatgatagaa cctgtaatat cagaacctcc acgtgagaaa
    24301 gtacagattt gattttcctt agtgacacca aagaaaccag gaatgacaag gacttcattg
    24361 gtgtttgtca attcttcaat cttgtcataa cttgatggaa tgatgcgagc gtgaccaggt
    24421 tcacttgtga ccacaatccc agcttctcta gggtgaatat agcgtgcatc gataccattt
    24481 tggttaaagt aggcagcaat caatttggca ttgttatttt caccggctgc taggaaagta
    24541 tcgtagagaa attcattttc ttcaatagga agagtggcca aggcgtgaat gcttttagaa
    24601 attttttcta gcacagctgg ttttagtccc aattcactaa ccatagcagc atagcggtcg
    24661 ataatccagc tttggctctt gctaatatcg ttacccgcaa catagtcgcg gtagtattta
    24721 atcagggcat ccgtaacctt agtatcttca gcattgcgtt taccaggcgc agaaacgact
    24781 acaaaacgac gatctgaatc gcttttgacg atgtttaaaa ctttttctaa ttgagtagca
    24841 gaggcaagag aactacctcc aaatttaaca actttcataa gaactcctaa agtaagtatt
    24901 ctatacgatt atagcagaaa gaaaggcttt tttcaatgaa gaaaatgcaa tcttgtttgg
    24961 gtgaaaaatg acaagtattt tgataataac ttgccaacat ctatcactta tcggaatgtt
    25021 aaaaaagtta gcgttttgct gttcaaattt gtaaaaatag t
//
LOCUS       DAVKDZ010000024        24259 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000024 DAVKDZ010000000
VERSION     DAVKDZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24259
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.024"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(176..430)
                     /locus_tag="VWZ30_000984"
     CDS             complement(176..430)
                     /locus_tag="VWZ30_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEU9334745.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(450..2291)
                     /gene="typA"
                     /locus_tag="VWZ30_000985"
     CDS             complement(450..2291)
                     /gene="typA"
                     /locus_tag="VWZ30_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU9334746.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            2453..3178
                     /locus_tag="VWZ30_000986"
     CDS             2453..3178
                     /locus_tag="VWZ30_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU9334747.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLKGDILVDSCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            complement(3218..3445)
                     /locus_tag="VWZ30_000987"
     CDS             complement(3218..3445)
                     /locus_tag="VWZ30_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334748.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(3534..3914)
                     /locus_tag="VWZ30_000988"
     CDS             complement(3534..3914)
                     /locus_tag="VWZ30_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9334749.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNKEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            complement(3914..4183)
                     /locus_tag="VWZ30_000989"
     CDS             complement(3914..4183)
                     /locus_tag="VWZ30_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEU9334750.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            4247..5155
                     /locus_tag="VWZ30_000990"
     CDS             4247..5155
                     /locus_tag="VWZ30_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9334751.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLSHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            complement(5209..5964)
                     /locus_tag="VWZ30_000991"
     CDS             complement(5209..5964)
                     /locus_tag="VWZ30_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU9334752.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(5985..6914)
                     /locus_tag="VWZ30_000992"
     CDS             complement(5985..6914)
                     /locus_tag="VWZ30_000992"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU9334753.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCAGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            complement(6929..7552)
                     /locus_tag="VWZ30_000993"
     CDS             complement(6929..7552)
                     /locus_tag="VWZ30_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU9334754.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKSFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            complement(7545..8783)
                     /gene="hflX"
                     /locus_tag="VWZ30_000994"
     CDS             complement(7545..8783)
                     /gene="hflX"
                     /locus_tag="VWZ30_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU9334755.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            complement(8776..9660)
                     /gene="miaA"
                     /locus_tag="VWZ30_000995"
     CDS             complement(8776..9660)
                     /gene="miaA"
                     /locus_tag="VWZ30_000995"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU9334756.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSETKMTIEGIHNRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVDQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            9781..9951
                     /locus_tag="VWZ30_000996"
     CDS             9781..9951
                     /locus_tag="VWZ30_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEU9334757.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            complement(9999..10838)
                     /locus_tag="VWZ30_000997"
     CDS             complement(9999..10838)
                     /locus_tag="VWZ30_000997"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU9334758.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLIQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(10935..11894)
                     /locus_tag="VWZ30_000998"
     CDS             complement(10935..11894)
                     /locus_tag="VWZ30_000998"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEU9334759.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDREKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPIACTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            complement(12021..13019)
                     /locus_tag="VWZ30_000999"
     CDS             complement(12021..13019)
                     /locus_tag="VWZ30_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9334760.1"
                     /translation="MNKRLFLKMSLATLPVLALFSQPVLAEENIHFSSCKEAWGNGYS
                     DIHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTATQINTSSATTSGWVKQDGA
                     WYYFDGNGNPVKNAWQGSYYLKADGKMAQSEWVYDSSYQAWYYLKSDGSYAYSTWQGN
                     YYLKSDGKMAQSEWAYDSSYQAWYYLKSDGSYAYSTWQGNYYLKSDGKMAKGEWIYDS
                     SYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGDDGVWKEGQASTA
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDD"
     gene            complement(13069..13686)
                     /locus_tag="VWZ30_001000"
     CDS             complement(13069..13686)
                     /locus_tag="VWZ30_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9334761.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSSYQKA
                     KERVYQHLAQETEPFVLIGLSLGAALALELSSYDLPNLRALVLSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMIGVSEELKTLDLTDIAGICPYPTLLICGSKDRPNL
                     SSMKTLHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKIRSFLELLK"
     gene            complement(13687..15408)
                     /gene="pabB"
                     /locus_tag="VWZ30_001001"
     CDS             complement(13687..15408)
                     /gene="pabB"
                     /locus_tag="VWZ30_001001"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU9334762.1"
                     /translation="MHRKTVIDFRALGERYTFTQPIKEIKTRNVAEVADLLAQVESYQ
                     KQGYYVVGYVSYEAAPAFEEKFAVHKAPLLGEYLLYFTVHDSVETSPIPLTYDEVDLP
                     SDWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVIEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNGRELATRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVEHLCQVEQYSTVWQMTSTIKSRLRADVDLI
                     TIFRSLFPCGSITGAPKIATMEIIKNLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYQEVHQKAAVLYRKQARFQLITTGKISQKNLLFEA
                     QHLERLRKASRYFASPFDAEDLKQKIKEECQDCDVNQDYRLRISLSKSGEIEVNRQVL
                     TPLSRTFCYAQLCLQEANLQQAFTYFKTSHRPHLGLRKQEIIYHNAAGELLETSIGNL
                     VLKISGRLYTPPIELGILPGIYRQHLLERGQVEEKVLTLADLAQAEAIYGCNAVRGLY
                     ELILQEN"
     gene            15595..16851
                     /locus_tag="VWZ30_001002"
     CDS             15595..16851
                     /locus_tag="VWZ30_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9334763.1"
                     /translation="MEQLHFITKLLDIKDPNIQFMDIINMDTHKEIIAKLDYDAPSCP
                     NCGSQMKKYDFQKPSKIPYLETTGMPTRILLRKRRFKCYQCSKMMVAETSIVKKNHQI
                     PRIINQKIAQKLIEKASMTDIAHQLSISTSTVIRKLNDFRFKHDFSYLPEIMSWDEYS
                     FTKGKMSFIAQDFKKLNIIAVLEGRTQAIIRNHFLRYDRAIRCQVKIITMDMFSPYYA
                     LAKQIFPCAKIVLDRFHIVQHLSRAMSRVRVQIMKQFERKSHEYKAIKRYWKLIQQDS
                     RKLSDKRFYRPTFRMHLTNKEILDKLLTYSEDLKHHYQLYQLLLFHFQNKEPEKFFGL
                     IEDNLKQVHPLFQTVFKTFLKDKEKIVNALQLPYSNAKLEATNNLIKLIKRNAFGFRN
                     FENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(16888..17196)
                     /locus_tag="VWZ30_001003"
     CDS             complement(16888..17196)
                     /locus_tag="VWZ30_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334764.1"
                     /translation="MKKLLGIVFLVAAATVVYFGSVGWPSLDVNLWSLIPVGLFLFFT
                     LENLLKKDYKASLMCLIIAFIIANAILDLLPISSGLVIGAGVLACVGIGYLFPDKEGK
                     "
     gene            complement(17680..24171)
                     /locus_tag="VWZ30_001004"
     CDS             complement(17680..24171)
                     /locus_tag="VWZ30_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU9334765.1"
                     /translation="MKKSTVLSLTTAAVVLAAYVPNEVVLADTSSSEDALTISDTEKV
                     VVDKEAENKEKHENIHNDIETSKDTEEKKTTVIEEKEVVSKESVIDNKTSNEEATIKE
                     DSNQSQEDKANSSASKDPESPKKEDKLVYIAEFKDKESGEKAIKELSNLKDTNVLYTY
                     NTIFNGVSIETTPDNLDKIKLIEGISSVERSQKLQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKGSMRFKKEDLKGTDKNFWLSDKI
                     PHAFNYYNGGKITVEKADDGSDYFDPHGMHIAGILAGNDTEKDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGENFKYRNIGAFFDKNKITTNEDGSKAPDKLKFVYIGKGQDQELIGLNL
                     KGKIAVMDRIYTKDLKNAFKKATDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEAT
                     KSQVFSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRVDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLEGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNKDSKGLVNSYGS
                     ISLKEIKGEEKYFTVKLHNTSDRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKIKGANITFEHDTFTIGANSSFDLNAVINVGEAKDKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSKSKTMEGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAAIIQNRKDKNITSLDQDPDLFAFNNQGIHAESTSG
                     SNVARVYPLDSKGEPQSVSLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENEPESKDNQDPATR
                     IRGQFESIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIISIDFSNPDKIKLIA
                     KDTYHKVKDEYKHETLFARDQKEHPEKFEEVANEVWYAGAALVDEYGDVEKNLDVTYA
                     GEGEGRNRKLDKDGNTIYEISGAGDLRGKIIEVIALDGASNFTKIHRIKFDDQADEKG
                     MISYYLVDPDKDASKYEKLGEISEEKLKNAKSPEENTNNNQAKDEDSKPDEESSVEGE
                     ASLEINKTISTIRDFENKDLKKLIKKKYREEYDFVTGGKRTVELDYQYDDKGNITAYE
                     DESALEYETEKLDDVKSKLGGVLSPSKDGHFEILGKVSNVSKNAKAYYGNDFKLIEIK
                     ASKYDPQTKTLTFDLFANTNDVVDGLSFTGDMNILVKDKGETKAKTKIRMPEKNKETK
                     TEYPYASSYGNVIELGEGDLSKNKPNNLTEMESGKIYSDSEKQQYLLKDNIILRKGYA
                     LKVTTYNPGKTDMLEGNGVYSNEDIAKIQKANPNLRVLSEKIIYADSRNVKDGRSTQS
                     VLMSALDGFNIVRYQVFTFNMNDKGEAIDKDGNLVTDSSKLVLFGKDNKEYHGEEYSN
                     VEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWEL
                     RVNESVVDNYLIYGDLHIDNTRDFNVKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGN
                     VYLQTGYSNLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYANGKSVVFNINDKRDN
                     GFNGEIQDKHIYVNGKEYTSFDDIKQLTDKTLNIKILVKDFARNTTVKEFILNKDTGE
                     VRELKPHTVTVTMQNGKEMSSKIVSEEDFILPVYKGELEKGYQFDGWEIAGFEGKKDA
                     GYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQR
                     EDHSQKSDSTKDVNATVPGRNDKNFENKTEVYPNEPENIANKDTNIDSKLTTNNLNKS
                     PKTGTVSGVFTSLVAGIMFTVGAFLGLKKKD"
BASE COUNT         6919 a         5282 c         4234 g         7824 t
ORIGIN      
        1 ttatttcaaa ataatatgca accagtacta agcaaatata aaatagattc cattaaagaa
       61 ttttattcaa gtttttccca ttcatattat ccaaacaaaa gagatggtga taactcaaaa
      121 ataaattgca atcttgtaaa attcctagca aaaaagagag ccgaaactct cttttttatc
      181 ttcttttact ttttttgact ggcatgagtg tgatgtctct aacactaaag taagctagga
      241 tcaacatggc tattgctagg aatatttctg ttggtaattg aaaaattttc agaaaagata
      301 gaaccaataa aatcaagagt gccactaaaa tacataccat agcgacgata ttgacagtcc
      361 ctttaatgct ttctggtgtc gcaaatacat agagtaggag cagtaaaatt cctaggacta
      421 aatagaccat ctttctctct ttctaactct tattcagctg attttttctt cttgttagct
      481 ttctcacgct ctgctttgtt aaggatttgt ttacgcaaac ggatagactc aggcgttact
      541 tccatgtact catcgtcgtt caagaactca agagactctt caagggtcaa gatacgaggc
      601 gtcttgataa cagctgtttg gtccttagta gctgaacgaa cgttggtcat ttgttttgcc
      661 ttcgtgatgt taactgtcaa gtcattttca cgagagtttt caccgatgat cattccttca
      721 taaacctcag tacctgggtt gacaaagatc gtaccacgtt cttcgataga catgattgag
      781 taagttgtag ccttaccagc atcgatagaa acaagggcac cacggtgacg tccaccaatt
      841 tcccctggaa tcaatggcaa gtattggtcg aaggtatggt tcatgatacc gtaaccacga
      901 gtcattgaca agaactcagt tgagtatcca atcaaaccac gcgctggaac aaggaagacc
      961 aaacgagttt gaccattacc agttgaaatc atatccaaca tttcaccttt acgttcagaa
     1021 aggctttgga taacagaccc ttggtattct tctggagtgt cgatttgtac acgttcaaat
     1081 ggttcacatt taacaccgtc gatttctttt acgataactt ctggacgaga tacttgaagt
     1141 tcatagccct cacgacgcat tgtttcgata aggattgaca agtgcaattc tccacgtcct
     1201 gaaacagtcc atttatctgg tgaatcagtt gggtcaacac gaagggaaac gtctgtttgc
     1261 aattctgcct gcaagcgttc ttccacctta cgagaagtta cccatttacc ttctttacca
     1321 gcaaatggtg agttgttgac caagaaagtc atttgaagag ttggctcatc gatgtgtagg
     1381 attggaagag cttctactgc atctgtcgga gtgatggttt caccgacaaa gatgtcttcc
     1441 atacctgaaa cggcaatcaa gtcacccgct ttggcttctt ggatttcacg acgttccaaa
     1501 ccaaagaaac cgaagagttt tgtaacacgg aagtttttag ttgtaccgtc aagtttagaa
     1561 agggtaactt ggtccccaac cttaactgta ccacggaaga cacgaccgat accgatacgt
     1621 ccaacgaagt cattgtagtc caaaagtgac acttggaact gcaaaggctc atctgagtta
     1681 tctactggag ctgggatatg gtcgataatc gtgtcaaaga ttggtgccat agtcgcttct
     1741 tggtcagctg gatcatctga caatgaagaa gttccgttga tcgctgaagc ataaaccact
     1801 gggaaatcaa gctggtcgtc atctgcacca agctcgatga aaagttccaa gacttcatcc
     1861 actacttctg ctggacgagc tgatggctta tcgattttgt taacaaccac gattgggaca
     1921 aggtcttgtt ccaaggcttt tttcaatacg aaacgagttt gtggcatggt tccttcatag
     1981 gcatctacga ccaagacaac accgtcaacc attttcatga tacgctcaac ttctccaccg
     2041 aagtccgcat gtcctggtgt gtccataatg ttgatacgag ttccgttgta agcaacggca
     2101 gtatttttag caaggatggt aatcccacgc tctttttcga tatcgtttga gtccatagca
     2161 cgctctgcca attcagtacg tgcatcaagc gtttctgatt gtttcaataa ttcgtcaacc
     2221 agggttgttt taccgtggtc aacgtgggcg ataatcgcaa tgttacggat atcttctctt
     2281 aattttgtca tgatttcctc tataatattc aaaatttatt ttctaactga acgattatac
     2341 cataatttca aataaataac ataactcaag caagtgtaaa tgttttcact ccgcttttct
     2401 tttcacgtca agccttttca aagcaagcga cttatgataa gataggcaca gtatgcgttt
     2461 agataattta ttagctcaag aaaaaatcag ccgaaaggcc atgaaacaag ccttactcaa
     2521 aggggacatt ctcgtcgata gttgcccagc ccgatcccta gcccaaaata tcgatacagg
     2581 actacaagaa ctcctttttc aggatcgaat cattcaaggc tatgaacaca cctatcttat
     2641 gcttcataaa cctgctggtg tcgttacagc caacaaagac aaggaacttc cgaccgtcat
     2701 ggacctgctt ccatctgata tccagtctga caagctctat gccgtcggcc gactggaccg
     2761 agatacaaca ggactccttc tcttgaccga taacggtccc ttgggctttc agctcctcca
     2821 tccccaatat catgtcgata agacttatca agttgaggtt aatggacttc taacacctga
     2881 ccatatccaa gcctttcaaa agggaattgt ctttttagat ggtactgtct gtaaacccgc
     2941 aaaactagag attctatctg caagtccctc cctcagtcaa gcctctatca ccatttcaga
     3001 aggaaaattt catcaaatca agaaaatgtt cctctcggtt ggtgttaagg taactagcct
     3061 caagagaatc caatttgggg acttcacatt gaacccagat ttagcagaag gtaactaccg
     3121 ccctttgaac caaaaagagt tacaaatcat taaaaactat ttagagatga gtcgataaaa
     3181 taaagaaagc tttaaaacta aagctttttt cttttattta ccgaaaaatt aaggcgattg
     3241 ctacaatcca gttaactaca gaaatcacaa ttcctaagat attaagaatc ttttctattt
     3301 tatagtctaa ttgtgactct ttttggtatg aaatagccaa gaccaatcct atgataccca
     3361 aaatcaggcc tacaattgga aataacaaac caagaataat cgacaagata cccacaaaaa
     3421 gtggattttt cttcttttct ttcatgttct aagaactctt taaattttat acaaattaat
     3481 tatactataa aacaatagct tcatcctatc attcgactaa tttggaaata aggttagcta
     3541 gtcttcactt tccctttcca agaatccaag ccataagaaa ggatataaat ctcagaaaaa
     3601 ccttgttttt tcaagtaaag agctgcattt gtaactcgtt gcgcacgttg gttttcgtag
     3661 agaaggacag gtttatcttt acgaagggct gcaagactag ttttcaactg acttgaagga
     3721 atattgcgtg caccaaggat atgttttctg tggaattctg ctgggtcgcg caaatcaatc
     3781 aattgacccg tacgaatcaa ggcttcaaac tccttattgt ccacaatttt agccgcacgg
     3841 cgaatacgaa gatagttaaa gcccatccac gccaacattg ctagtataag tgcccacaaa
     3901 atccaagtaa ccattagttc ttttctccat ttttctcaat ataatccaat tctaccttgt
     3961 gctctctgcg aagaactgct tctgcctcta gatagtctaa tttatccatc aaccctgcat
     4021 cgtaaatccg agatagttcc aacttcatca gttcaatatc atataagcgt tttcccatgt
     4081 aaacaataat accaaatcgt ttgaggaatt gctgcacatc atagaatgtt ttcataagac
     4141 tcattctagc aaaattttgt gtttttttca agaagagact cacacaatgc tccttatttt
     4201 cctatcttct ttagcgattc taaggcaagt atggtacaat aaaaacatga gaattcaaca
     4261 attacattat attatcaaaa tcgtcgaaac tggctccatg aatgaggcag ccaagcaact
     4321 ctttatcact cagccaagtc tctccaatgc agtgcgagat ttggaaaatg aaatgggcat
     4381 tgagatcttt atccgcaatc ccaagggaat caccttgacc cgtgatggga tggagtttct
     4441 ctcctatgcc cgtcaggttg tcgagcagac ccagcttctg gaggaacgct ataaaaatcc
     4501 tgtcgcccac cgcgaactct ttagcgtttc gtctcaacac tatgcctttg tggtcaatgc
     4561 ctttgtctct ttgctcaaga aaagcgatat ggagaaatac gaactcttcc ttcgtgaaac
     4621 tcggacttgg gagattattg acgacgtcaa gaatttccgt agtgaggtcg gtgtcctatt
     4681 tttgaacagc tacaaccgtg atgttttaac caagatgctg gatgacaatc acctgctatc
     4741 ccaccatctc ttcacagcgc aaccgcatat ctttgtcagc aagaccaacc ctctggcaaa
     4801 gaaagacaag gtgaaactgt ctgatttgga gaatttccct tacctcagct atgaccaagg
     4861 gacgcacaac tccttctact tttcagaaga gattctttct caagaacacc acaagaaatc
     4921 cattgtggtc agtgaccgtg ccaccctctt taatctcttg attggtttgg atggttatac
     4981 cattgcgaca gggattttga acagcaacct aaacggagac aatatcgttt ctatcccact
     5041 ggatattgat gacccgattg agctggtcta tatccagcat gagaaaacca gcctatctaa
     5101 gatgggcgaa cgctttatcg actatctact agaagaagtt cagtttgata gttgagaaat
     5161 gataagaacc aatatgtagg ctagcaacaa cctgcacatt ggttcttttt acttataatt
     5221 aaaagtttcc cctgccaact tatcagctag cttgggaaag agagtataaa acttatgggc
     5281 taggttcaac aaaatcggga gattgagttc tcgtttattt ttgcctataa tcttgacaat
     5341 ctttttagcc actgcatctg cctctaagag gaagcggtca accgatttaa gataagttcc
     5401 atctgggtca gcttggtcaa aaaatcctgt tcggattgga cctggattga ctgttgtcac
     5461 atagactcca tagggcataa gttcgagtcg cagagcattt gaaaaaccaa tagccgcaaa
     5521 cttggtcgct gagtaaagac tagacttgcc agtagctatt agacctgcca tgctgacgat
     5581 gttgatgata tgccctttgc tgctttcctt catacgagcc gcaaggtgac gagacagatt
     5641 catcagggca aaggtattga cctcaaacat ctggtgaata tctttatcag caatctggtc
     5701 aaatccctca aaaatcccgt aaccagcgtt gttaatcaag acatcaatct tgccatagcg
     5761 gagataaaga tcagttacca gagcttctag ggctgaatcg tcggtaatat caatttcaat
     5821 caattctgca tgggaataat ttccgtagag ttgggctaat ttttccttat ttctaccaag
     5881 caagatgagt tggtcattgg gcaggagttt gaccatttct tgagctagac caccgctagc
     5941 tccggtaatg agaatagtag gcatacttat cctttctgtg actgctagat ttccacttct
     6001 tccaagtctt tgaccacatg gacattttca aaaattgtgg cagcgtcttt cttgagtttg
     6061 ctaatatctt ttgagaggaa acgggcactg atatggttga gtaggaggcg tttggcacct
     6121 gcttctaccg ctacttgtgc agcttgcata ttagttgagt gaccatggtt acgagcaatt
     6181 ttttcatcac ccttgccata agtggactca tgaactagga catctgcatt gacagccaga
     6241 cgcacactgg catccgtttt tcgagtatct cctaaaatag tgataatctt acctggacgt
     6301 ggcgctgaga tatagtctgc tgccttgatt tcagttccgt cttccaaaac aagatcctgg
     6361 ccgtttttga ttttaccaaa aagcgggccg aacggaacac cagcagcctt gagtttttca
     6421 gcatccagcg tcccttctag atccttttgc atgacacgat agccagcaca gaaaatagtg
     6481 tggtccagct cctctgcata cacagtgaat ttatcggttt caagaatttt acccagagaa
     6541 tcttggtcaa actcatggaa atgaatgcgg tagggcagac gagaacctga cacacgaagg
     6601 ctggttaaga caaatgactt gattccttga ggtccgtaga tttccaaatc tgtctgctct
     6661 tcattggcct gaaaggcacg gctagaaagg aaacctggca aaccaaaaat gtggtctcca
     6721 tgcagatggg taataaagat tttgctgacc ttacgtggtc gaattgtggt ttccagaatg
     6781 cgattttgcg taccttctcc acagtcaaag agccaaactt cgttaatctc gtccaaaagt
     6841 ttcagggcga gacttgaaac gttgcgggct ttagagggct gaccagcccc cgttcctaaa
     6901 aattgaatat ccattcgata ctttctaatt aatcaatata taacatggct gtgcggtttt
     6961 ccgatcggaa atagcgtttg ccagaaaaag cagcagcttc ttgcaataaa tcctcttggc
     7021 tgtagccttt gagacgtttt cgaccatcag ccaatctttc caaatcagtc aaagctgtga
     7081 gactttctag gctgacaact tcctcgtcct cgacaggctt catgtaaatc ttaccagact
     7141 cttcaaagac taattgatgg gggaaaattt gcgcaatttc aaagagcaag tcatccgaga
     7201 ttttctcctc attttcagag aaaatccgac caaagccgtc actctcataa caaaaaccaa
     7261 aggatttacc agacagatta agccgaataa aagacttatt ttctagggtg aaacttggct
     7321 cagtattgta aagattcagt tcctgactga gttctgcaaa ataatccgtc gcagcctgag
     7381 gacttttttt ctgatagagt tctgcaaagt aggcattaac aacacttggc ggaggtgtaa
     7441 taagtgttaa ctgctcctgc tctgtcttac cagctagaag ctgatccaga tagaccttgt
     7501 ccagacttgt ataacctcca tactttagag ccaaagtttt aatatcagtc ataaaattct
     7561 tctaacctcc atttattttt ctcggaaatg tagcctgtaa tcacttcgcc gtcttcctga
     7621 taatcacgtt cttccagaat tgcaacactc tctaaatcat gaatcttgta ggactttgaa
     7681 aaaggcactc gcagggtaaa tgcttcaaaa atttccttaa tcttatctag caataatgct
     7741 tgcaagtttt cacgactgtc ctcagacttg gcagaaatga gggtatatgg cgtttgggta
     7801 ggcgtgaaat cctccaccaa atccgcttta ttataaagcg tcaagtgagg aatatcttcc
     7861 atgtccaggt ctttcatgat ggagagaacc gttttttcat gttcctcgtg gtaaggattg
     7921 ctagcatcga taacatgaac cagaaggtcc acatgcttgc tttcttccaa ggttgacttg
     7981 aaactggaca ccaactctgt cggcaaatct tggataaagc caacggtatc tgtcaaagtt
     8041 acttggaggt tgcctcccag atgaatactc ttggttgtcg catccagagt cgcaaagagc
     8101 tcatctgctt catactgggt cttactggtc aagatgttca tgatagttga tttcccagca
     8161 ttggtataac caatcaaacc aatcttaaaa gtgctagact ccaaacgttt ttctctgaca
     8221 gtcgcacgat ttttctcaac caccttgagc tggcgctcga tatccgtgat ttgattgcga
     8281 acgctacgac ggttcagctc cagttggctt tcaccaggac cacgggaacc aattccccct
     8341 gcctgacggc tgagcataat cccctgacca accaagcgag gcaagaggta tttgagttgg
     8401 gctaggtgga cttggagctt cccttcatgg cttcgagccc gcatggcaaa gatatccaaa
     8461 atcaactgca tacggtcaat gaccttaaca ccgagaactt cctctagatt gacattctgc
     8521 cttggggtca gacggttgtt gacgatgaca gtagtgattt cttctgcatc caccataagc
     8581 gcaatctctt ccaacttacc agagccgacg aaggtcttgg aatcatattt ttcacgtttt
     8641 tgtctgtagc tatctacaac gactgcccct gccgttttcg ctaaactagc caattcttcc
     8701 atggagaggt caaaactgtc cataccctgc aattccacac caatcagcag gactcgctcc
     8761 tcttttttct ccgtttcaat catctaaaaa ctcctctatc tggcttaaaa tgcggtcttg
     8821 tacaccagat tctccaatct gataaaaggt gacctgcatg cgattacgga accaggtcag
     8881 ctgacgcttg gcaaaacgac gggtcgcctg tttgagactc tcactagctt cctccaaggt
     8941 ctgctctcca cggaaataag gaaagagttc cttatagcca attcctttag cagcctgtac
     9001 attaggggaa tggtcaaaca gccacttggc ttcatccaaa agcccagcct caaacatcaa
     9061 atccactcgg tggttgatac gctcataaag ttgactacgt tcatcatcca agcagataat
     9121 cagcggttca tacaaggtct cttgattttc caaatcctga ccaaaatggg caatttctaa
     9181 ggcacgcata gcacgacgac gattaaactg gggaatctca aggcctgctt gatccaccaa
     9241 atgggctaat tcctcatctg aatatggctc caaactagct cgataagcta aaatctcctc
     9301 atgaggagtc tctccaccta ggtggtaacc ttctagcaag ctctggatat aaagtccagt
     9361 cccaccggcg ataatggcta gcttgccacg gttgtgaata ccctcaatag tcatcttagt
     9421 ttctgaaaca aaatcaaaag ccgagtaaga ctcggttatc tctctaacat cgattaaatg
     9481 atgaggaaca gctgcctgct cttctggact agccttggcc gtcccaatat caagtcctcg
     9541 atagacttgc tggctatctc cactaaccac ttcgccatta aaacgctttg caacttcaat
     9601 agctagagcc gtctttccaa cagcagtcgg tccaacaatc acaattattt ttgttttcat
     9661 cttttttcct tgaaaaattc ccattttttc gttactatta ttataacaca aaaaggtcag
     9721 tcagaaaaat gtgacctctt gaggaggctg gctgattgag aatataaagg aggaagactc
     9781 atggctaaag gattcgctaa aggtcttgta acaggtgtcg caggaactgt cgctgccgtt
     9841 gcaggtgcag tatacgcatt taaaaagaaa gtaatcgaac cagaagagca aaaagcagct
     9901 ttcatcgaag aaaaccgtaa aaaagcagct cgtcgccgcg tatcacgtta agaaacaaaa
     9961 gaagttggga ttattctcaa cttctttttt ctattctttt aaatagctag gtcaaacttc
    10021 aactgtggct taacagggtc ataatccacc aactcaaaat cttctgcttt gatatcaaag
    10081 aaattagtcc catcaggaac atttaaaacc aagcgtggtt ggcagtttga cggctcccga
    10141 cggagcaatt cctgagcttg ttcaaattga ttatcataga tatggaggtt gttgatgaag
    10201 tagaagaact tcccaacctt ccagccaaaa tgtttggcaa tcatcatctg caaagccaca
    10261 tactgcatag cgttgatgtg gtgggctact agcatgtcat tggagcgctg gatcaaggtc
    10321 gcatccagat agatttcccc atcaacacgc cggacatcaa acatggtctg aaaggcgcac
    10381 gggagcagcc catctgtttc ttcgaaagct tggtaatccc agagcgaaat aatattgcgg
    10441 cggttccaag gattggtttc caactgtttg agaagcttat tgataatgtc gtgtttctta
    10501 acaacggcac catagcgctc accaatggtt cccgtgtctc caacttccca gtcattccag
    10561 tagtgaacat tgtacttgtc attaagcact tctaagctat ttgactggtc ttggtaaatc
    10621 cagagaactt ctttgatggc ggatttgatt gcaatgggac gcaaggttgt gataggaaat
    10681 tctcctttag ataagtcgta ctcggaaaag gcaccagtta catacttgga attagcaaca
    10741 gtcccatcct tgtacttagg acgagcctgc tctgagaaaa caccttcctt tagaatgcgt
    10801 tcaatattct ctttaaaaat cgtatctgct tttgtcattt cgtttcacct cattcattgt
    10861 cctaactagt atagcataaa aaataaaaga ggaacattac taactctagg ttagcatttt
    10921 cctctctcat tatcttattg caatacaagt gatgctgctc cgataactcc agcgtcattt
    10981 cctagagttg caagagctaa tttagtagat gtgcgtactt gtgggaaact attttcatcg
    11041 taaacttttt gaacaccttg tagaaggaat tctcccgcag ctgacacacc accaccgatg
    11101 acgattgttg atgggtttag gattgaacca atgttagcac aagcgattcc caagtaacgt
    11161 gagaagttac ggtaaacaat caaagcaagg tcgtctcctt cttttgcgag atcaaagaca
    11221 gtcttagcag ttacttcttc tccgttatca atcaagcgtt tcaaggctgc atcgccttcg
    11281 tattcatcgg catagcgacg agtcaagttg acaatccctg ttgctgaagc aactgtctca
    11341 aggcagcctt tcttaccgca agtacatgcg attggctggt caaagtcaac agtgatgtga
    11401 ccaagctcac ctgctgcacc agcaacaccg tgaagcaatt tgccttctgc gacgataccg
    11461 ccaccaacac cagtaccgag tgtcataaag acaacgtctg gttggttatc tccagcaccc
    11521 atccagcgct caccaagagc tgctacgttg gcatcattat cgatgaaaaa tggaatgccc
    11581 aaagcttttt caatcttttg tttgattggt tgaagggttt tccagttcaa gttgtaggca
    11641 ccgataacag tccctttttc acggtcaacc acacctggtg atcccattcc aatgccttgg
    11701 aagtccgctg ctgccaatcc aagcaagtcc aaacgatgtt gaatagactc aatcatatca
    11761 tcaacgatat gacttccctc atccaaaatg tttgtcttga ttgaccattt tccttggatt
    11821 tctcctgctg ttgttaagat tgcaaatttg atagaagttc caccaaggtc aatcccaata
    11881 atcttttgac tcatcatatt ctcctttttc attgtgtagt aattgaaaat gcttacagta
    11941 ctagtatatc aaaatccatt cacttatgca aaggtttgcg taaatgaatt ataaggaatt
    12001 ttttcgccta ccagtatctg ttaatcatct aaatgatcaa tggcatattg agctgcatca
    12061 tttgaaaatt tatcaaaatc agaagttaat tgtctataca tacgtttttt tgacatgtgg
    12121 aataacgaat tataactttt tgcctttcct aaagcagcag aatattcact attgctatca
    12181 ttactagaag aagctgtact tgcttgacct tccttccaaa ctccgtcatc gccaacataa
    12241 taacgtccac catcaaccca ttcattgaca gccattttac catccgattt tagatagtaa
    12301 tttccttgcc atgtactgta agcataagaa ccatctgatg tcaaataata ccaagcttga
    12361 taagaagagt cataaatcca ttcaccttta gccattttac catctgattt caaatagtaa
    12421 ttgccttgcc atgtactata agcatacgat ccatctgatt tcaagtaata ccaagcctga
    12481 taagaagagt cataagccca ttcgctctgg gccattttac catctgattt caaatagtaa
    12541 ttgccttgcc atgtactgta agcatacgat ccatctgatt tcaagtaata ccaggcttga
    12601 taagaagaat cataaaccca ttcgctctgg gccatttttc cgtctgcttt caggtaatag
    12661 cttccctgcc aagcattttt cactggattt ccatttccat caaagtagta ccaagcgccg
    12721 tcctgcttaa cccaaccact tgttgttgct gaacttgtgt tgatttgagt agccgttgac
    12781 tgttttgctt taaaagcacc cttaggagca tttttcaatt cgcaagccac accatcatga
    12841 tcacggtcta acttggcaga ataaccaggt tctccctcgt gaatatccga atagccattc
    12901 ccccaagctt ccttacagct cgaaaaatgg atgttttctt ccgccaaaac tggttgtgaa
    12961 aacaaggcta aaactggtaa tgtagccaga ctcattttta aaaatagacg tttgttcatt
    13021 tgtttctcct ataacgataa tatcgatttt agtattattt tacaaaactt atttcagcaa
    13081 ttcaaggaaa cttctgatct tttctacaaa ctcctttggt tttgccttat tcaagacatg
    13141 agggccatca ggaatgatct gaaactgcga atctgtcaac agtctatgga gagttttcat
    13201 agaactaaga ttcggtctat ctttgctgcc acaaattagt aaggttggat aggggcaaat
    13261 ccctgctata tccgttaaat caagtgtctt caattcctca gaaactccga tcataagagt
    13321 cttatctgct ccctgttttt caaataccct tttgggaagt agtttaaaaa tcagcaactg
    13381 aagataaaat agaatattcc ctgctaattt aagcgggcat cctgatagaa ccaaagctcg
    13441 aagatttggt aaatcgtaac tggaaagttc tagtgccagg gcagcaccta aggacaatcc
    13501 aatcaaaaca aaaggttctg tctcttgagc taggtgttga taaactcgct ccttggcttt
    13561 ttgatagctg ctaactccag aaggaaataa ctcaatagcc tcagaaggat aatctgtcag
    13621 tagatttcga acttctttcc aagactctgc tgactgccct aacccatgta aaaatatcag
    13681 tttcatctag ttctcctgta agattaactc atacaagcct ctcactgcat tacagccgta
    13741 aatagcttct gcttgggcta aatctgccaa ggtcaagact ttctcttcta cctgtcctct
    13801 ttccagcaaa tgctgacggt aaattcctgg caagattcca agttcgatag gcggtgtgta
    13861 gagtctccca gaaattttca gaaccaaatt tccgattgag gtttccaata gctctccagc
    13921 tgcattatgg taaataattt cctgcttcct taggcccaag tgaggtcggt ggcttgtttt
    13981 gaagtaggta aatgcttgtt gcaaattggc ttcctgaaga cagagttggg catagcagaa
    14041 ggttcgactg agaggtgtta atacttgacg attgacttct atctctccag acttgctaag
    14101 gctaattcgc aagcggtaat cttgattaac atcacaatcc tgacactctt cctttatctt
    14161 ttgtttcaag tcttctgcat caaaaggaga agcaaaatag cgactagctt ttctcagcct
    14221 ttccagatgt tgtgcttcaa acagcaggtt cttctggctg attttcccag tagtaattag
    14281 ctgaaaacga gcttgtttac gatagagaac agctgccttt tgatgaacct cttggtattc
    14341 agattcccaa gtgctatccc atgtaatgcc tccgccaact ccatagattg cttgaccttt
    14401 gtgaagttga atggtacgaa tggccacatt aaaaatccgt cgtccatttg gaagcaagag
    14461 accaatcgtt ccacagtaga ctccgcgtgg ttgaggctct aaatttttga taatctccat
    14521 agtcgcaatt ttcggagctc ctgtgatgga tccacaagga aagagagagc gaaaaatggt
    14581 aatcaagtca acatctgctc gcaaccgact cttgatggtc gaggtcatct gccaaacagt
    14641 agaatactgc tctacctgac acagatgctc cacgtgctca ctgcccactt cagaaatacg
    14701 gttcatatca ttgcgcaaga gatccacaat catcatgttt tcagagcgat ttttggggtc
    14761 ctgttctaac caactggcct gttcaagatc ttcttggtca gttaccccac gctgggtcgt
    14821 tcccttcatt ggtctcgtag ccaattcacg gccattttgc tcaaaaaaga gctctggact
    14881 catggaaatc actgccatct cgtcatgttc cacataggca ttgtagcccg cctcctgctc
    14941 tatcaccata cgattgtaga tggcaaaagg attggcattt aacttttgct taagttggac
    15001 ggtgtagttg acctgatagg tatctccctg ccgtaaatga tgatgaattt gggcaatcgc
    15061 cttctcatag tctgctgcag acgtcacttc ctgccaatca gaaggcaaat caacctcatc
    15121 ataagtcaga ggaatagggg atgtttctac actatcatga actgtaaaat atagcaggta
    15181 ctctcccagt agaggagcct tatgaactgc aaacttctcc tcaaaagcag gagcagcctc
    15241 gtagctgaca tagcccacca cataatagcc ttgcttttgg tagctttcca cttgtgccag
    15301 caagtctgcc acttctgcta catttctcgt ttttatctct ttgataggct gggtaaaggt
    15361 gtatctctcc cccaaagccc taaaatcaat cactgttttt ctatgcatac cttaagtata
    15421 gcataaaaag gcagatcaag aaaaacttaa tctacctttt tatgatttta tggctctata
    15481 atatttgtag tgggtaaccc ccctatggat attacggagc ctattttgtg tagaaaaaaa
    15541 atcccataag atctataatg aaaagcgaca aaacaactca ttagaaagaa tcatatggaa
    15601 caattacatt ttatcacaaa actccttgac attaaagacc ctaatatcca atttatggat
    15661 atcatcaata tggatacaca caaggaaatc atcgctaaac tggattatga tgccccatct
    15721 tgtcctaatt gcggaagcca aatgaagaaa tatgactttc aaaaaccatc taaaatccct
    15781 taccttgaaa cgactgggat gcctactaga attctcctta gaaaacgccg attcaagtgc
    15841 tatcagtgct cgaaaatgat ggtggctgag acttctatcg tcaagaagaa tcaccaaatc
    15901 cctcgtatca tcaaccaaaa gatcgctcaa aagttgattg aaaaggcttc tatgaccgat
    15961 attgctcatc agctgtctat ttcaacttca actgttattc gcaagctcaa tgacttccgg
    16021 tttaagcatg atttttctta cctccctgag attatgtcct gggacgagta ctcctttacc
    16081 aagggaaaga tgagctttat tgcgcaagat tttaaaaaac tcaacatcat cgctgttctt
    16141 gagggcagaa cacaagccat cattcgaaat cactttctgc gctacgatag agctattcgt
    16201 tgtcaggtaa aaatcattac tatggatatg tttagccctt attatgcctt ggctaaacag
    16261 atttttccgt gtgctaaaat cgttctggat cgctttcaca ttgttcaaca cttaagtcgt
    16321 gctatgagtc gtgtacgtgt tcaaatcatg aagcagtttg agcgaaaatc ccatgaatac
    16381 aaggctatca agcgctactg gaaactcatt caacaggata gccgtaaact cagtgataag
    16441 cgattttatc gccctacttt tcgtatgcac ttaaccaata aagagattct tgataagctt
    16501 ttgacctatt cagaagactt gaaacatcac tatcagctct atcaactctt gctgtttcac
    16561 tttcagaata aggaaccgga gaaatttttc ggactcattg aggacaatct aaagcaggtt
    16621 catcctcttt ttcagactgt ctttaaaacc tttctaaagg acaaagagaa aatcgtcaac
    16681 gcccttcaac taccctattc aaacgccaaa ttggaagcga ccaataatct catcaaactt
    16741 atcaagcgca atgcctttgg ttttcggaac tttgaaaact tcaaaaaacg gatttttatc
    16801 gccctgaata tcaaaaaaga aaggacgaaa tttgtccttt ctcgagctta gcttttcttc
    16861 aacccactac agttgacaaa gagcctatta ttttccttct ttatcaggaa agaggtaacc
    16921 aatacctaca caagccagca cacctgctcc aatcaccaag ccacttgaaa ttggcaacag
    16981 gtccaaaatt gcatttgcga tgataaaggc aataatcaaa cacatcagac tagccttgta
    17041 gtcttttttc aaaagattct ctagagtgaa aaagaggaac aatcctaccg gaatcagtga
    17101 ccagagattg acatctaaac ttggccagcc aacagaacca aaatacacta ctgtcgctgc
    17161 tgctactaaa aagacaatcc ccaataattt tttcatttct ttatctccga tttctttttt
    17221 aggaacttga gttgtctaat atcgacgcct tgcgtctctt acaaaaatca ttatagcaga
    17281 ggtcagtttc aagaacaagt tcgtttgcct atctggtctc aatctcagtc taagtggttg
    17341 aataatagcg ataaaataag gaagcaggtg aaaaacttcc ttccaaataa aaaggaatta
    17401 agcaaccaaa cactacttaa ttccttgaaa ttatctcatc acctctattg acctttaaat
    17461 tttgatagac tctattttct aatcaatatt gtcaatacta accctttcaa tcaattcatt
    17521 agttgtcgaa actaagtcta ttaatagatc caaaaagagt atcaatagta aagtaaacta
    17581 aataattgag ttataaatag ctattttcac aaaactgttt catcatcata ctatctcagt
    17641 acataaacca ttttttcatc tatagctttt gtcttaatct taatcttttt tctttaatcc
    17701 aagaaatgcg cctacggtaa acattattcc ggcaaccaat gacgtgaata ctccgctaac
    17761 tgttccggtt tttggtgact tattaagatt gttagtagtt aatttactat cgatattggt
    17821 atctttattg gctatgttct ctggttcatt aggataaact tcagtcttat tttcaaagtt
    17881 tttatcattt ctaccaggaa ctgtagcatt gacatcctta gttgaatcag atttttgtga
    17941 atgatcttct ctttgtaaat cctcttttct gtgactttca tttaattgac tatggtttac
    18001 ttgtgggtta tctttctttt tcgatacatc aaaagtaggt ttattttctt cctccttttt
    18061 ctcctctatt ttcttgaata caggttttat aaaggtatct tttgatagat taataacata
    18121 gccagcatct tttttacctt cgaaaccagc aatttcccaa ccatcaaatt ggtatccttt
    18181 ttctaattca cccttataga caggtaaaat gaaatcttct tccgacacta tcttagaact
    18241 catttctttt ccattttgca tggtcacagt taccgtatga ggttttagtt ctcttacctc
    18301 tcccgtatct ttgtttaaaa tgaattcttt tacagttgta tttcttgcaa aatcttttac
    18361 aagaatctta atgtttagtg tcttatctgt tagttgttta atatcatcaa atgatgtgta
    18421 ttcttttcca tttacataaa tatgtttgtc ttgaatctca ccattgaatc cattatctct
    18481 tttatcattg atattaaaga ctacagattt tccgttagca tattcgatac ttgtatttcc
    18541 cttgggatca atgtttactt ctggtttaac attatcatat aaaaattgat agtgtactcc
    18601 aaccgctttc gcattcaaat tgctatagcc agtttgaaga taaacatttc catccatatc
    18661 tgttacctta tctggaaatc cgtttgcttt ataatctttc atcccccagt ccatgatgtc
    18721 accgtcttta acattaagct taacgttaaa atctctagtg ttatcaatgt gtaaatctcc
    18781 gtagattaaa taattatcta caaccgattc attaactctc aattcccagt taaaaccacc
    18841 cttatcagaa atcttacctc ttaaataaaa ttctggattt cgtacataaa ttttattaga
    18901 tttagatgga ttaaagtagt tcttatccat tgaaaggttt actggtttgg tatcaataaa
    18961 taacatggag ccatcttctt ttatagcttc tacattagaa tattcctccc catgatactc
    19021 tttattatct ttaccaaata atacaagttt agaggaatct gtcacaagat ttccatcttt
    19081 atcgattgcc tcccctttat cattcatgtt aaaggtaaac acttgatatc ttacaatgtt
    19141 aaagccatcc aaagccgaca ttaatactga ttgagtgctt cttccatctt taacatttct
    19201 actatcagca taaattattt tttctgaaag gactcttaga ttaggattgg ccttttgtat
    19261 ttttgctata tcttcattgc tatagactcc atttccttct aacatatccg tttttccagg
    19321 attataggta gtcactttta gtgcatagcc ttttctcaga atgatattat cctttaatag
    19381 atattgttgt ttttctgaat cagaatagat tttaccagat tccatttcag ttaaattgtt
    19441 tggtttgttt tttgaaagat caccttctcc taattctatt acattcccat aacttgatgc
    19501 atagggatat tctgttttag tttccttatt tttttcaggc attctaattt tagttttagc
    19561 ttttgtttca cccttatctt ttacaaggat attcatatca cccgtaaagg atagaccatc
    19621 gactacatca tttgtgtttg caaataagtc aaaggttaga gttttagttt gtgggtcgta
    19681 tttacttgcc ttaatttcta ttaatttgaa gtcattgcca tagtaagcct tagcattttt
    19741 ggatacatta cttacttttc caagaatttc aaaatgtcca tcctttgatg ggcttaatac
    19801 tccaccaagc tttgatttaa catcatcaag tttctcagtt tcatattcta gagcactctc
    19861 atcttcatag gcagttatat ttcctttatc gtcatattga taatcaagtt ctactgttct
    19921 ttttccacca gttacaaagt catattcttc cctatatttc tttttaatga gtttctttag
    19981 gtctttattt tcaaagtctc taattgttga aatagtttta tttatttcaa ggctagcttc
    20041 tccctcaact gaactttctt catctggttt tgaatcttca tccttagctt gattattatt
    20101 ggtattttcc tctgggctct ttgcattttt gagtttttct tcagaaattt cacctagttt
    20161 ttcatattta gaagcatctt tatcaggatc tactaggtaa taagaaatca ttcctttttc
    20221 atcagcttgg tcatcaaatt taattctatg gattttggtg aagttgcttg caccatctag
    20281 ggcgattact tcgatgattt ttcctcttaa atctcctgct ccgctaattt cgtagatagt
    20341 atttccatct ttatcaagct tcctatttct tccctcaccc tctccggcgt aagttacatc
    20401 aagatttttc tcaacatctc catactcatc gacaagagct gcacctgcat accatacttc
    20461 gttggctact tcttcaaatt tctcaggatg ttctttttgg tctctagcaa acaaggtttc
    20521 gtgtttgtac tcatccttta ccttgtggta ggtatctttt gcgatcaatt taattttatc
    20581 tggattagaa aaatcaattg aaataatttt tggagctgta ttatcaattt ttactggtat
    20641 ataagaaact tgccatggat aatccttggt taatctatat ttgaatttgt agaagtattg
    20701 accctcagct attgattcaa attgtcctct tatcctagta gctggatctt ggttatcctt
    20761 actttctggt tcattttctt ctctacctct agggttatag atgagtccat cccacttcaa
    20821 atcaccccaa acttttagtt tagatgattt gattcccttt gcatcattgc ttttagaatt
    20881 taaaattcct ctaataaagt gttctctcga aatgactttt aagtctcttt gattttctcc
    20941 ctctttattt gtatttacta ttgaaatcaa tccttcttct gcacttctta atacaagtgg
    21001 agaaggtgtt aatcctcttt ctagtgaaac actttgtggt tctccctttg agtcaagtgg
    21061 atatacccta gcaacattag atccactagt ggattctgca tgaatacctt ggttgttaaa
    21121 ggcaaataag tctgggtctt ggtctagtga tgtaatattt ttatcttttc tgttttgaat
    21181 aatagctgct ggattaaatt tatctatacc atgttctcca ccaataccct tgttcaaggt
    21241 tccaggaatt ttcggtttac catcatcatc ataaccttcc attgtttttg attttgaacc
    21301 ctcttcccag gcccatttat caaggattgg ttcgtggttc caattcccag caaatcccat
    21361 tagaggcatc gacaaagaag gttggaagtt tattttcttc ccgttagagt ttagagcttc
    21421 catttcttcc actgactcaa aatgaataaa tgattctaca aatttatttt tgtctttagc
    21481 ctctccaaca tttataaccg cattcaaatc aaagctagaa tttgcgccta tagtgaaagt
    21541 atcatgctca aatgtgatat ttgctccttt gattttttct ggatggattt ctggaacaat
    21601 ttgcttccca tctggagatt tttcatcttt atatgtttca tccagtttta atctgtcagt
    21661 tagagaatct gtagttatcg ctgatgctga aactttaaag gttaaaggtc tgtctgatgt
    21721 attgtgaagc ttaactgtaa aatatttttc ttctcctttt atttctttaa gagaaataga
    21781 accataagag tttaccaaac ctttagaatc cttgtttttg aaagtcgcta caacttcatt
    21841 tctcaaagca ttggcaacat taattagccc tgctccctgt tgtctaggtg atgcaaagta
    21901 ttggcttttt tctttccaag acgttgcgtc catcataggt cgcgcagtat tttgtaaagc
    21961 aatttttgta agacttgtaa ggtctatttt gtcatctccc tcaagatttt tcaatacagg
    22021 tctttcaagc atttccttta acttcggtct aatcaaaaca gtagaagctg ctacgattgg
    22081 agttgccata ctagttcctg acatatagcc ataagttgat ttaccattaa tgacattaag
    22141 cgtggattta atatttttac caggtgctga aacatcgggt tttaaaagta aatctactct
    22201 tggcccccaa gatgtggatc ctgctggaac gatgatatct tctttataga gttctttgtc
    22261 tgtgtcaggt gcaaacttaa agtcaatctc tttttcgtca cctacattcg gtttgttgga
    22321 attaaaactt tccatatcaa ttggatagta ttgctccaat ttatctttaa aatcttcttt
    22381 attatttctt ttgacttcag tttttttatc aggattaatc atgttccata gctttacacc
    22441 atcatctcct gaaattgaaa acacttgact tttagtagct tcatccgctt catatcccat
    22501 agctggaagc tctgtccaat tatctctatt gtagtaattg acagtattta caaccataat
    22561 ggcgcgtgcg cccttatccg tagctttttt aaaagcattt tttaaatcct ttgtataaat
    22621 tctatccatt actgcaattt tgcccttaag attcaaacct atcaactctt ggtcttggcc
    22681 cttgcctata tatacaaatt tcaatttatc aggagctttt gaaccatctt catttgttgt
    22741 gattttattc ttatcgaaaa aggcccctat atttctgtat ttaaaatttt ctccacctat
    22801 gttgacttta tcaaactcaa ctgtctgatt tttagcagaa gcgaccgcta tcgcatcttc
    22861 atgtgctgca gttcgcgtta catttccagt atctgtcatt ttcagatgat tatttgccac
    22921 taaatcccat gaagagcttg aagcagaagt cgcatagtta cccgtagcta caaccattgg
    22981 aatgcctgct tttcttaatg ctctaatagc ttgccaatat ttctcaccta caagacctgt
    23041 tcctgtaaag ccagatgata ccgaaacaac atcgacattg tgtttgatcg aatcttcaat
    23101 agcatgaaac attgtttcat ctcctgcgaa gccagatcct gcgtcagagt acattttata
    23161 agagaatatc tgtgcattag gagcaattcc atctattcca ttaaagttct tgatgtcttt
    23221 ttcagtatca ttccccgcaa gaatccctgc aatatgcatc ccatgtggat caaaataatc
    23281 gctcccatca tcagcttttt ctacagttat tttaccacca ttataataat tgaaagcatg
    23341 aggaatctta tcactcaacc agaaattttt atcagtacct tttaagtctt cttttttaaa
    23401 tctcattgag cctttagcat catcatcaat cctcatggcc ttatgcctat aatctgtccc
    23461 ggtatcgata tttgaaatga ccatacctct accatcaaaa tttttaccaa atggagcatt
    23521 gatagacttt aggtaatcga tagcctcctc aactccaatt tcctttcggg catgattcat
    23581 catgggttgg agtttttgtg accgttcaac cgatgaaata ccttctatta gtttaatttt
    23641 atccagatta tctggagttg tttctattga aacaccatta aaaatcgtat tataggtata
    23701 taaaacgttt gtatccttaa gattggataa ttccttgatt gctttttctc cagattcttt
    23761 atctttaaat tcagcaatgt agacaagttt atcctctttt ttgggacttt ctgggtcttt
    23821 acttgcagac gagttagctt tatcttcttg ggattgattg gaatcttctt tgattgttgc
    23881 ttcttcattg ctagttttgt tatctatcac agattcttta ctaacaactt ctttttcctc
    23941 aataaccgtt gttttcttct cttcagtatc cttggaagtt tctatatcat tatgaatatt
    24001 ttcatgtttt tctttatttt ctgcttcctt atctactact actttttctg tatcagagat
    24061 agttaaagca tcttcagagc tagatgtgtc tgctaagact acctcattag ggacatatgc
    24121 tgctaaaaca actgcagctg tggttaatga caatactgta ctttttttca ttttaactcc
    24181 ttacatattt atttaacttc taatagatag aaaactttaa ctttgctagc ctttgttata
    24241 aaaagtttta ctaagtatt
//
LOCUS       DAVKDZ010000025        21663 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000025 DAVKDZ010000000
VERSION     DAVKDZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21663
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.026"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(1..456)
                     /locus_tag="VWZ30_001005"
     CDS             complement(1..456)
                     /locus_tag="VWZ30_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334766.1"
                     /translation="MGAARDFLELINYKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNAIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
     gene            complement(782..2470)
                     /locus_tag="VWZ30_001006"
     CDS             complement(782..2470)
                     /locus_tag="VWZ30_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEU9334767.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQLFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DHHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPALYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
                     DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSA
                     LAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIRKYA
                     SVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALASNC"
     gene            complement(2633..3466)
                     /locus_tag="VWZ30_001007"
     CDS             complement(2633..3466)
                     /locus_tag="VWZ30_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU9334768.1"
                     /translation="MDYKEKLENKAKEYFEYVETKITEKALDATHFAEEASREQYDAV
                     VVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLEINQDIDGAIDELDFDLT
                     NKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILTYAVNTMKSVMTDQVFNI
                     KVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANILILKDASIFSKLSVIPDL
                     LKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPVEIKVLAQRVEVFSKPKE
                     D"
     gene            complement(3628..4482)
                     /locus_tag="VWZ30_001008"
     CDS             complement(3628..4482)
                     /locus_tag="VWZ30_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU9334769.1"
                     /translation="MLGAITGDIVVSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            complement(4540..5298)
                     /locus_tag="VWZ30_001009"
     CDS             complement(4540..5298)
                     /locus_tag="VWZ30_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9334770.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMEIRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYELDIGVTRDGRTLVVEVNDGYALGNCGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            complement(5599..6165)
                     /locus_tag="VWZ30_001010"
     CDS             complement(5599..6165)
                     /locus_tag="VWZ30_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334771.1"
                     /translation="MVILELYQNDYSKDLVAFNSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESCGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            complement(6433..7791)
                     /gene="rlmD"
                     /locus_tag="VWZ30_001011"
     CDS             complement(6433..7791)
                     /gene="rlmD"
                     /locus_tag="VWZ30_001011"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9334772.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTDSFI
                     KASAQTGVDRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVECVSLLEK
                     RN"
     gene            complement(7865..8839)
                     /locus_tag="VWZ30_001012"
     CDS             complement(7865..8839)
                     /locus_tag="VWZ30_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEU9334773.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVTFFKSFGS"
     gene            complement(8913..9527)
                     /gene="pmp23"
                     /locus_tag="VWZ30_001013"
     CDS             complement(8913..9527)
                     /gene="pmp23"
                     /locus_tag="VWZ30_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEU9334774.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSISG"
     gene            complement(9527..10501)
                     /locus_tag="VWZ30_001014"
     CDS             complement(9527..10501)
                     /locus_tag="VWZ30_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEU9334775.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSENLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(10509..11765)
                     /locus_tag="VWZ30_001015"
     CDS             complement(10509..11765)
                     /locus_tag="VWZ30_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU9334776.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVESLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMALIEPGDTVMGMDLAAGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFRETADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEDLAKKINSAIFPGIQGGPLEHIVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQAPDFRIISGGTENHLFLVDVTKIVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     AENEAVLEEVRSAVKELTDAFPLYED"
     gene            complement(11826..12257)
                     /locus_tag="VWZ30_001016"
     CDS             complement(11826..12257)
                     /locus_tag="VWZ30_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9334777.1"
                     /translation="MLRDLRETDVKAICEINQEALGYSFSPEDTASQLAKLSQDSHHF
                     LLGYEDAASHVLLGYVHAEVYESLYSKAGFNILALAISPQAQGQGIGKSLLQGLEQEA
                     KRRGYGFICLNSADHRLGAHAFYEKVGYTCDKVQKRFIRIF"
     gene            complement(12259..12861)
                     /locus_tag="VWZ30_001017"
     CDS             complement(12259..12861)
                     /locus_tag="VWZ30_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEU9334778.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDCVYQLKRR
                     PRDKALNLNVASLEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TVGFRMPSHPITLDLIRETGPLIGPSANISGQTSGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTILDLSGDKVKILRQGAIKREDILAQLPEISFEEE"
     gene            complement(12845..13684)
                     /gene="prmC"
                     /locus_tag="VWZ30_001018"
     CDS             complement(12845..13684)
                     /gene="prmC"
                     /locus_tag="VWZ30_001018"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU9334779.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLATHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLEENLQVLDIGTGSGAIALALAKNRPAWSVTAADISQDALELASENAKNQNLQ
                     IFFKKSDCFTEISEKYDIIVSNPPYISREGESEVGLNVLYSEPHLALFADEDGLAIYR
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPALFKKHLPEKRVRTLKDQFGQDRMVVVDD
                     GQD"
     gene            complement(13684..14763)
                     /gene="prfA"
                     /locus_tag="VWZ30_001019"
     CDS             complement(13684..14763)
                     /gene="prfA"
                     /locus_tag="VWZ30_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU9334780.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(14760..15350)
                     /locus_tag="VWZ30_001020"
     CDS             complement(14760..15350)
                     /locus_tag="VWZ30_001020"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU9334781.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            15473..15655
                     /locus_tag="VWZ30_001021"
     CDS             15473..15655
                     /locus_tag="VWZ30_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEU9334782.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            15792..17165
                     /gene="mnmE"
                     /locus_tag="VWZ30_001022"
     CDS             15792..17165
                     /gene="mnmE"
                     /locus_tag="VWZ30_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU9334783.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(17212..18147)
                     /gene="dapA"
                     /locus_tag="VWZ30_001023"
     CDS             complement(17212..18147)
                     /gene="dapA"
                     /locus_tag="VWZ30_001023"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU9334784.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(18203..19279)
                     /locus_tag="VWZ30_001024"
     CDS             complement(18203..19279)
                     /locus_tag="VWZ30_001024"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU9334785.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(19864..19936)
                     /locus_tag="VWZ30_001025"
     tRNA            complement(19864..19936)
                     /locus_tag="VWZ30_001025"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19901..19903),aa:Thr,seq:ggt)
     gene            complement(20036..20545)
                     /locus_tag="VWZ30_001026"
     CDS             complement(20036..20545)
                     /locus_tag="VWZ30_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU9334786.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(20543..20639)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            20668..21099
                     /locus_tag="VWZ30_001027"
     CDS             20668..21099
                     /locus_tag="VWZ30_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU9334787.1"
                     /translation="MKIPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQILIIIINYILSKIYIFRK"
     gene            21181..21558
                     /gene="mscL"
                     /locus_tag="VWZ30_001028"
     CDS             21181..21558
                     /gene="mscL"
                     /locus_tag="VWZ30_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU9334788.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKEE
                     KTAEKKPTELEVLQEIKALLEKK"
BASE COUNT         6336 a         4650 c         3909 g         6768 t
ORIGIN      
        1 ctaaaagtaa gattccattg tatttccttg ttggtctttc atatcacgaa ttttattttt
       61 cagaatatta aagttacaaa tttcatctct aatatcgtta tccgctctgt agccattaat
      121 catctcagtc agagttttcc ctttactacc attctttata gtagtattta ctctttttat
      181 actgctttca aaaggctcat atgttttata cttaattact tcgtctttaa aattaaataa
      241 aggtgtatcc ggcttagcat tccataaaat aatcaaccac attatagcat tataaagctg
      301 ctttgcagtt tcattccact tttcaggttg taaatttcca ctccctgtat tgtcatattc
      361 ttgtctatta tttacatgag aatcacctac aagcggatta aaaagaggta gccctttgta
      421 attaattagt tctaaaaaat cccttgccgc acccatgcaa cactgaataa cttccttctt
      481 gccatcaatt ttactctcag cgataaatac ataatattca tcttcaatag ttgctcctgc
      541 atcactatgt ttttgttgat ttgctaacag cttaatatgt gctttaggct aaacttcaaa
      601 atctctaaca attctttgtc ttgtttcatg tcctctacaa ttcatttaag ttctccttta
      661 caagtttagt aactagcctt attatactat ataagatatg aaatgtacca taatcttcat
      721 caactagttt ttagaaaatc acaaatccat ctggagagat gacaagctga ttctccaatt
      781 cttagcaatt gcttgctagt gctactgctt ctttctctaa aagataatct tctgttgatt
      841 ggttgaatat tgctttgagg atccgtcctt cgtatttcca ttccagagct actagatcag
      901 atttgatttc ttgaaggcta tacttgccat gcgaaatgat tactgacgcg tatttccgaa
      961 tcttaattag cctcttcata aagttcagca tatcattgtc acttgataca cgttcccaag
     1021 gcatacaacg acggcaatct ggatctggtc ctccagtcaa ggctagctcg gttccgtaat
     1081 aaatgcacgg tgttcctttt tgtaaaaaga gaaaggctaa ggctgattta accagttgaa
     1141 catcttcatt ggccgtccac aggattcgct ctgtatcatg tgaatccaag agattaaaca
     1201 tgacctctga aatctgctgc ttgtaataca tagactctcc attgatttca tcgatgaact
     1261 ggtctgtctt cttaattcct cgtaagaaat agtccttgat actatcagat aaaggataat
     1321 tcatgacggc atggaactca tctccattta gccaaggctg agatgtatgc cagacttctc
     1381 ctaggatata aagagcagga tttttagcta aaactgcctt acgaaaatcc ttccagaact
     1441 gatggtcaat ctcattagcc acatccaaac gccaagcatc gatattaaac tcttcaatcc
     1501 aataagtcgc aacctttaaa agataattct tgacctctgg attggctgta tttagcttag
     1561 gcatatagtc ctcgaaacca aaaacatgat agggtaagtc tctcttatta actagctttt
     1621 cagttgtcac tgggaattgt tgaatatgga accaatcctt ataagcagac tgttcaccat
     1681 ttttgacgac atttttccat tgaagagatt gcgaaccaat atgattaaat accgcatcca
     1741 gcatgacttt catgccacga tgatgcgctt gatccaccag ttcccgaaag gtctccttgt
     1801 ctccaaaatg atggtcaatt tcaaagtaat ctgtcgtatt gtacttgtga ttgcttgtag
     1861 attcaaagat gggacaaaga tatagtccag taatacccaa gtcttgcaag taatccatat
     1921 gatcaataat cccctgtaaa tcaccaccaa agaaatcatc gctcttaggt gtgacagatg
     1981 aatcccagtc taaagtccct tctgggttta atagagcatt gccattggca aatctttcag
     2041 gaaatagctg ataccatacc gtatttgaaa cccagtcagg aaccttgcag gcatcaatct
     2101 catgaagata aggcaactta aatccattcc caattgcatg aagattttct agagaatttt
     2161 ccacacaccc tttatcgcca tacaaaatat tttgaccttc tgtatctctg agctcaaaga
     2221 gatactggat acgtgcaaag tcaactgaca cttcaacctg ccaatggtca aataaggtac
     2281 cagaagttat cttgaccatt tcttttgtat cctgataaaa ctcctccata aagataaaag
     2341 ggtccccata gtgcaagttg atgctttcaa tgtccccttt cttagttcga attcgaatat
     2401 ggagtttctt atccttataa agataggcat actccgactc aggcctatgg taaatagcac
     2461 tcaattccat caatttgtct cctaaagtat tcattcaaat tttacgcaaa cgttttcaaa
     2521 cttaatgaat ccagtatact atttttgtaa actatttgca aggaatgtcc gttgttaaag
     2581 gaactatctt ttaaagtata caaaaagcct taaaaaaggc tttctctata tactaatcct
     2641 ctttcggttt tgaaaatact tctactcgct gagctaggac tttgatttct acaggtaggt
     2701 tatctgattt atctccgtca acatctgact ccaattcact atctgaagag attttaatat
     2761 tacgcgcttt gatatactcg atattatcat ttgcgacaac atcccctttt aataaatcag
     2821 gaatgacgga taatttggag aatatagagg catctttcag aatcaaaatg ttggcatagc
     2881 cgtccttgtt ttcttcaaag attttcttat cagcgaagta atttgtcaaa aggaccaaaa
     2941 catggctagc ttcaccaaca taatttccat tttctgtctc aaccttaatg ttaaagacct
     3001 gatctgtcat gacagacttc atggtattta cagcataggt taaaataccg aattttgttt
     3061 tgtcctctat ttcaacattg tgaatcgcct caggcagaga accgatacta aagatataac
     3121 caaaatagtt gtcatttgct ttaccgatat caatcttatt ggttaaatca aaatccagtt
     3181 cgtcaattgc gccatcgatg tcttgattga tttccaaaag ttttgtaatg aggttacccg
     3241 taccgcctgg gataatccct aacttaggaa tgtagtctct ctcatcaata cctgaaatga
     3301 cttcattgac agttccatct ccaccaaaca caaccactgc atcatactgc tcacgagaag
     3361 cttcttcagc aaaatgtgtt gcatccagcg ctttttcggt aattttggtt tcaacatatt
     3421 caaagtattc ttttgcttta ttctccagct tttctttgta atccaaagcc ttctcgccac
     3481 agaagtaggg ttgataatta ccattgcttt tttcattgat tttatcctta attttaaaca
     3541 gaaatgttta catttcgtcg tatgcaagta aatgtaatcc tattatacaa tgaaaataca
     3601 gaaaagagaa atctgacgta ctggagatta atacgctttt attctatttt cccatcgcct
     3661 aactacatcc tttaagggtt catccaagta agaataggcc ttatccttga tccaatcagg
     3721 aataccgtaa gctgcctctg ctatgctaca agtgattgct gcgagagtat cactgtcgcc
     3781 accaagtgag atggcatttc ttatcgcatc ttcgaagtct ctactttcaa gaaaggcgat
     3841 aatggcttga gggacagttt cctgacatgt ttcgttaaaa cgatagttag gacggatttc
     3901 atctaaagtt tgagatagat tgtaatcgta ttctttttct atataatcct taattcgtct
     3961 tttacactct gtaggattgt cgttaattga ttgctcgtac tctctacaat aaccaccaaa
     4021 gtaaaaacga catagaaaga tagcatcagc tgtagccata gcgcctttga caccttctgg
     4081 atgattatga gttacctctg cagaaagact cgtaagtcct ctagatgatg gccatatacc
     4141 agttttcgca taaaaaccac agtccatgat ccaagcacat ggagaaatac gcatagctga
     4201 tccattccca aagctattat aaggctcacg gttatcgctg tttagccatg cattaaaccg
     4261 agcaccgtaa tcagcattcg gatacattct gccatatttc ttcatcgcgt caatgaagtc
     4321 gtctttttgt ccaccattca taattgcttc tgcaacagca caggtcataa ccgtgtcatc
     4381 tgtaaaaaag cagtccttcc gaaataaagg aaagtccttt gttttgatat tgttccattc
     4441 gtaaacagaa acgacaatat ctccagtaat tgctccaagc atcagattcc tccttgttca
     4501 ttttgatgct ttttatattg gttatctacc atatttattt tagaaaataa catcctgttg
     4561 gattttaaaa atttcatttt tttcaaaata gggttttacc atttctttcc acctagctct
     4621 atgaaaattg attgatttta aaggagatag gccacaattt cccaatgcat aaccatcatt
     4681 tacttcaaca acaagtgttc tgccatcgcg agtaacaccg atatctagtt cataagctat
     4741 tggcgcatct ttccaacatg atatcgcttc atcaattaca cttgcatcaa attgtgcatg
     4801 ataatcacct gtatagggtc gaacatctaa tacgcgacca tctaacacaa aacaacgcca
     4861 ttcagctatg aattctacaa cctcactaat ccatatagga tagtcgaaag gtagaccaat
     4921 acctattaaa tcatgggttc cattaacaac tcttccagta aagacttttg aaccagcttt
     4981 aggcttaata aattttcccc aattatcagg tatattcaca atctctccta aaataccagc
     5041 ataaatcttt cgaccataaa actctttaag ctcaatagga taatcataaa ccggaacgtt
     5101 taagcccatc atttttagta atgctctaat ctccattata taatctacaa ctatatcttc
     5161 acttgttaac tcttttattt cagaaaaaga ttgatataaa ataacttctt ctccttgtaa
     5221 gtaggcacct acttgagcat tgtatttatt aattgaaacc tcacttggta atttactttg
     5281 tctaatataa acaaccattt catcactcct atatcactag tgtcaactgg cttttataat
     5341 acattagttt aaaagtggag aggattttta acacagtaac tttaaatctt tggtattaaa
     5401 aaattttcac aatatttata gaaataaaat ctgtctcaaa tcagttatca aatctagtat
     5461 aaattatgag cggccactct aatactttcc ctctaaacaa gaaaaagact tacactcaag
     5521 ggttttctta cccccttcgt tataacgttt tgactctttt actagcaaag gtatatattc
     5581 acaaggaact ttggttgact attgaatctc tccaacttct tctttaacat atccttctac
     5641 atcttcaatc tctacaaaca ttgggtctaa gtgacacaag aaatgccaaa cttcgatccc
     5701 tttttttctg taaagaatcg cttcaccgtc ttcacttccg aaaaagcttc tgtcgatttc
     5761 atatccgcag ctttctaaga agtcttttgc tttacgatag ttcgtttctc ttgtttcgac
     5821 ataggcttta acttcatggt tgttaacgac atatgcatca atttttgaat atccttcgat
     5881 cactctatca tttttgaggg ataaatttga aatctctttc caaataatgt ttacattttc
     5941 ctcaggatcg aacataaatt tagataaagg aacaatattt ccgttaaaaa taatttccat
     6001 ataatccggt atgtttttag gattaaaata ctccacttca aaaccatctt ctgtttccag
     6061 agtgtatccc gggatttgag ctacaaaggc tttcccatct tctatggaat taaatgctac
     6121 taaatcttta gaataatcat tttggtacaa ttccaatata accatcgata atctctccat
     6181 tttcattatc aggctaatgt aaataagcac gtcacctgac caattcaggc tctctgtatc
     6241 atctcatcat atttcctact tactttacga gtcctatacc cagaacacac cttatcgacc
     6301 ttcggtctca cctcgtcgca ttggctgaac atctactttt acttcgctga tgcttcaact
     6361 cgtacaagca gtgataccgc ctcagcgtga tgcctcagtg ggactcaaaa ggttcaggga
     6421 accttttgag gattaattgc gtttctctaa caaactcaca cattcgacgt gatgcgtttg
     6481 aggaaataga tccaccggct ggactttctt caattcatat cccaattctt ggtagagttt
     6541 aatatcacgc gccatggttg cgacattgca ggaaatatag gcgatgcggt ctactcctgt
     6601 ttgagcgctt gctttgataa agctatcggt caatcccttt cgtggtgggt cgaccaagat
     6661 aacggttggt tgaatacctt ccttgagcca tttcttcatg gcattttcag ccgtgtcaca
     6721 gacatagtgg gcgtttgaaa tattgttcag ctgagcattt tttttactat tctcaaccgc
     6781 ttctggaatc agttcaacac cgtagacttc tttaacatgc ttggcgactg ataaaccaat
     6841 ggttccaata ccagaataag catcaataac cacgtcatct ttttttaact ctgcaaagtc
     6901 aatggctgtt tgatagagtt tctccgccat ttcagtattg acttggtaaa aggctgggcc
     6961 agcgatttgg aagtcatttc ccaacatctg gtccgtaata tagtcttgac cataaagagt
     7021 gcgccactcc ttaccaaaaa tcgcattggt attctggtcg ttgatatttt gcatgacaga
     7081 cacaatctct gggaactgct tgataacttg ttcaatcaat tggtcaacac gaaaaacttt
     7141 tggacgagtt gtcaccaaaa cgaccatgat ttgtcctgaa tagtgaccac gacgcaccac
     7201 aagattccga atcaatccag actgttcctt ttcgtcataa ggttttaaat caaaacgacg
     7261 gagcaggtct cgaagagcta ctacgacttg gtcaatgaca ggatcctgga taaagaaatc
     7321 ttcaaggggc atgaggttgt gcgaattctt acggaaaaat cctgtttcca agacaccatt
     7381 cactcgacga acgggcacct gcgccttatt gcgatacttg actggatgtt ccataccaag
     7441 cgtttcagca acttctacat ctgcaattcc agcaatcttg tagagactgt ccttgacttg
     7501 cttggtttta aacttgagct gttctggata agaaaggtgt cctaaatccg cgattcctga
     7561 acgcaggtaa gccaaatcta gatcttgatt acggtgtggt gactggacaa ggtatttttc
     7621 aacttttcca aagccaatct ttttattgac cttgaggaca cgcatgagaa ttttttcact
     7681 cggtaaagca ttctctacaa aaaagaccaa accatctacc ttggcaactc ctgccccttc
     7741 atgggtcaag tcaacaattt caacttctac aatatcattt ttctttaaca ttcttttctc
     7801 tttctattgg ccgacaaaaa agacggcaac gttacggtta ccatccatta taccatgaaa
     7861 tttcttaaga accaaaactc ttgaagaagg tgacaagggc ttgccagagg gagcttaaga
     7921 agttaccacc gtcctctagc gccttatccg catcaaagtt aaggttgata tttttaaaac
     7981 tgtcgccagc ttgtgatacg atgctttgtt taaggtcatt tagggtttta gtgaaatctg
     8041 cattgctgag gatatcactc tttgagagat tcaaggcaaa attgatgatg atattgatct
     8101 ggtttcctgt tatgacctga tcaagtttgt aattttttaa ggtatcttca acaatcttgc
     8161 ggatatcttc ttctgtcaga tttcccttgc tttctttagc tttggcgagt cctgacttga
     8221 tatcagctag ggcaacgttt aatttattag catcatagcc tgatttgtcc ttgttttcag
     8281 cattgatatc tgacaaagcc ttcaactctt cttgagccaa atctttatta gcttgtggca
     8341 ccttggctcc attagcctct agcgaatagt aaatccctgc taaagcactt tctcctgtaa
     8401 ctggaatagg ggctgctaca gtgattttgg catgttccat acccagcgtt actgctgcgt
     8461 ttcggtacat atcctgagtc accttagtga tattttctgg tgtttcaatc ttgacctcaa
     8521 gtggcgattt gtcacctagc ttttgaatct tggctgatga atacaactgt aagctagagt
     8581 cattggccac attcatgatt ttagaataaa catcaggtgt catggtcttg agttctttgg
     8641 tatctgttga ggcattgtac cccagttttt taagagtttg atttttttgg tcttcagata
     8701 gggaagaacc taggacatat tcaggttgga cataggtttc atcgataact ttttgaacat
     8761 ctgttgctgc atggacgcta ttcatagctg ttactgccca caagatcgca gcgctagtca
     8821 gaaagagttt ctttctcata gggaatttcc tcctttactt ctttagagta atatatctat
     8881 cttaaagaaa acttataaca aaaacacctg gtctagccag atattgaaaa gagagtgaaa
     8941 catttgatga tgtaaaggtt aagtcgtacc tgtctagaat aataatagtt tcctccattt
     9001 acatagagtt cagcaccgtg aaaaatggaa atggggtgaa tataactata agtctttcca
     9061 gtcgtattac caagcaaggg ggcaacagtc tcacgagagt actgtttggc tagagccaag
     9121 gtattttcct tgccattttg ggcgataaaa tcgatatagg caggtccaaa attataggct
     9181 tgaacagctg tccagatatc tacacccttc ttctgcgcca gatagagatt gcctgtcaga
     9241 gtttgaatgc cttgccgaat gctagaggca ttatcattga tggtgttggt tgaaccactt
     9301 gcagactcac tagactgcat aacatcgcct tcttttcctt ttgtttcagt ataaatcata
     9361 gcaagcacaa gctcttcgtt tgctggagtg tctttttcac tcaagatttc tcgcaccatg
     9421 ggttgatagg tcatgacttg tttgacatct tgatgaacgc ggtaagcttt atagccagca
     9481 aaaaggaaga ctgctagtac aagcactctt cgaattcgtt taaacattat ttactttgga
     9541 tatcctcgat atttttgatt aagatagagt aggttccatt ttcgttttgg ataaactcaa
     9601 cagactcggc gtcttgatag acgttattgg gaacgatgag ttcaattcca tttgataagg
     9661 agagtttttg gttttcaaat ttctttaatt ggcgactggc atcaatttca tcaaattgaa
     9721 caggttctgg tacggcttct ttgacttggt caataaagct caaacgagcc gtcagattgt
     9781 tgtcaaaaag gtcattagcc aatttctcag gtgacaattc attgctttct tctagattgt
     9841 tgaaaatagc tgatttgacc ttggattgaa attgaaaatc atctgtgtta aaagattcgg
     9901 caattctctg ggctgttttt tccagttcct tgatggattt cttaggtgaa atcttggggg
     9961 caacagctag gagattttct gaaaaatagt tcaaaaaagt cccgttgtac ttgattcgtt
    10021 tttcaatcag atggtacttg cgactctgaa gattgactac caaggcctcg tcagcacccg
    10081 ttccaaatcc aggcaggtta ttctgagtca gcttgattgg attatcaact tctcctccga
    10141 ggtgggtcaa ggtctcccgc agggcaattc gcaagaaagc gaaatgttct acaccttctt
    10201 tagaaaattg aacaaaaatc aagtcattgg tcttgagatt ttcagaaatg ctaaactcct
    10261 ctttccagag attagccagc gttactgatg tctccaacaa atcgtctgta atatgattga
    10321 agaagggatt ttcttcttcg aaaatcccag tcttggcttc atctgaatac acatgttcaa
    10381 tttttttacg caggtattct tcgatttttg gagtaatatt gagaaactta tctgctaaga
    10441 acagctcggt atcatccgga ctgaactggt gaataatggc tttcttaata taaatgtcca
    10501 taaaagtttt agtcctcgta taatgggaag gcatctgtca attctttgac tgcacttctc
    10561 acttcttcta atacagcctc attttctgca tttttaaggg ttttaatgat gagttcagcc
    10621 actttgcgac tttcttcttc accaaatcca cgtgcagtaa tggctgctgc tccgatacga
    10681 atcccacttg tcttgaatgg tgacaaggtt tcgtaaggga ttgaattttt atttagggta
    10741 atattgactt catccagcaa gttttgagca acttttccgt tttctacaat cttagtcaca
    10801 tcaacaagga agaggtggtt ttcagttccg ccagaaataa tacggaaatc aggggcttgc
    10861 aagaagacat ccgccatagc cttgctgttc ttgataacat tggcagcata ttccttgaag
    10921 gctggatcca aaacttcttt gaaggaaact gccttagcag ccacaatatg ctccaaagga
    10981 ccaccctgaa taccagggaa aatagctgaa ttgattttct tagctaaatc ctcatcattg
    11041 gtcaaaatca aaccaccacg aggaccacga agggttttgt gggtcgttgt tgtagtgata
    11101 tgagcgtatg gcactgggct tgggtgaaga ccagctgcaa ccaagccagc gatgtgggcc
    11161 atatcaacca tgagcttagc cccaacagca tctgcggttt cacgaaattt tgaaaaatcg
    11221 ataatttgag aataggctga agcaccagct acaatcagtt ttggttttac ttcttgggct
    11281 tgtttcaaga tagcatcaaa gtctaagagt tccgtttcag gatcaacact ataagaaaca
    11341 aagttgtagg tttgaccaga gaagctaaca ggagccccat gagtcaaatg accacctgct
    11401 gccaaatcca ttcccataac cgtatcacct ggctcaatca aagccatgta agccgcacag
    11461 ttagcctgac ttcctgagtg aggttggaca ttggcaaatt tagcaccgaa aatttctttt
    11521 gcgcgttcaa tggctagaga ttctaccaca tctactacat cagttccacc ataataacgg
    11581 cgtcctgggt aaccctcggc atatttgttt gtcaagatag acccttgagc tgccataaca
    11641 gccttggaaa ctacgttttc cgaagcaatc aactcgatat tgttttgttg gcgttcttct
    11701 tctttggcaa tagcattcca gagatcagca tcgtatgctt taaaatcatc tttatcaaaa
    11761 atcataggtc ttctccttta ttttgtgact agtccattag tttgatttta caataagaaa
    11821 ataaactaaa agatgcgaat aaaccgtttc tgcactttat cacaagtata accaactttt
    11881 tcataaaatg catgagctcc cagacgatga tcggcagagt ttaagcagat aaacccataa
    11941 ccacgtcttt tggcttcttg ttccaaccct tgtagtaaac ttttaccaat accttgacct
    12001 tgcgcttgag gtgaaattgc taaggctaag atattaaatc ctgctttgga atagagtgat
    12061 tcgtaaactt cagcgtggac atatccaagt aaaacatgac tagctgcatc ctcatagcca
    12121 agaaggaaat gatgggaatc ctgagataat ttagctagtt gactagctgt gtcctctgga
    12181 ctaaaagaat aacccaaagc ctcttggtta atttcacaaa tggctttcac atcagtttct
    12241 cttaaatctc ttagcatctc attcctcctc aaaagaaatc tctggcaact gagcaagaat
    12301 atcttctcgc ttaatggccc cttggcgtaa gattttcacc ttgtctccag acaaatccaa
    12361 aatagttgaa tcctgtccag ttagaaaagc atcgtcttcc agacccagaa cttcttggtc
    12421 aaaatccttc aaaatttgtt caaaggttac accacttgtc tgacctgaga tattggcaga
    12481 cggcccaatc aagggacctg tctctcgaat taaatccagt gtgatagggt gactgggcat
    12541 ccgaaatcca acagttgcaa ggtcagaatt tacccaatag ggaactcggt cattggcttc
    12601 gagaataatg gtcaaggggc ctggcaaaaa ggtctctaca agtttttgta gataagctgg
    12661 ctgattcttt gaaaagtgca agatgtcctc taaagaggca acattgagat tgagcgcctt
    12721 gtctctagga cgacgtttga gctggtaaac acagtcaact gctttttcgt ctagcgcctt
    12781 ggcaaaaaga ccataaaccg tctctgtagg tagaacgaca gctccaccat tttccaactc
    12841 ttgtctaatc ctgtccatca tcaaccacga ccatcctatc ttgaccaaat tggtccttga
    12901 gtgttcgaac tcttttttca ggaagatgtt tcttaaaaag cgcaggaaca ctttgacctt
    12961 gcttgtatcc aatttcaagg taaatcttac caccatcttt gagatagtct tttgcatctt
    13021 ccgcaattct acggtaaata gctaggccat cctcgtctgc aaagagagct agatgaggct
    13081 ccgaatacaa gacattcaag cctacctctg actcaccttc acgagagata tagggtggat
    13141 tggaaacaat tatatcatat ttttcagaaa tttctgtaaa acagtcagat tttttaaaaa
    13201 atatttgaag attttgattt ttagcatttt ctgatgcaag ctctaaggcg tcttgggaaa
    13261 tatctgctgc tgttactgac caagctggtc tgttttttgc taatgctaga gctatggcgc
    13321 cacttccagt cccgatatct aggacctgaa gattttcctc aagattttca gttagaataa
    13381 gctccactaa ctcctctgtt tctggacgag gaatcaaaac ccgttcatcc acctttaaat
    13441 gcattccata aaaatctgcc tgtccaatga tgtactgagc tggcttgtga gttgctagtt
    13501 gctggtaaat atcttctaca aattgttttt cttcctctgt tgtcacctcc tgctggaggg
    13561 caaaaataaa gtctgtaaaa gatagatttt tcagactacg atagacaaaa gagaggcttt
    13621 ccgcttcctc tccttgtctt atcaactctt cttcaaaatt tgaaaataat tgagctaatt
    13681 tcattatttg tttaattctt ctagtttttg tgtttggtca taaagcacca aggcatccac
    13741 aacttcgtcc aatttaccag acaaaatcgt atctagtttt tggagggtca agccgatacg
    13801 gtggtctgtg acacggtttt gtgggaagtt ataagttcgg attcgttctg aacggtcacc
    13861 agtaccgatt gtcgacttac gctcagcgtc ctgctcatct tgagcaatct gagcaaagtg
    13921 gtcagcaaca cgggcacgaa tgattttcat ggccttctca cggtttttct gctgggtacg
    13981 ttcttcttgc atctcaacct tgatattggt tggcaagtga acgatacgca cggcagtcgc
    14041 aaccttattg acgttctgac caccagcacc tgatgcgtgg taaatatcaa cacgaagatc
    14101 ttttggatcg atatcgtatt ctacttcttc gacttcaggc atgacaagaa ctgtggcagt
    14161 tgaggtatga acacgacctt ggctttctgt cacggggaca cgctgcacac ggtgggcacc
    14221 tgattcatac ttaagcttag agtatacaga ctgacctgaa accatagcaa ccacttcttt
    14281 aaaaccaccg acaccattca tagaggcttc catgacttca aagcgccaac cttgggcttc
    14341 cgcatacttt tggtacatag ttagcaaatc tccagcgaaa agtgccgctt cgtctccacc
    14401 agctgctcca cggatttcaa ggatgatatt cttgtcatcg tttggatcct ttggaaggag
    14461 caaaattttc agtttttctt catattcttc tttttcagcc ttggcatctt tgagttcttg
    14521 cttggccatt tcttccaagt ccgcatctcc gcctgattcc ttaatcatct cttcggcatc
    14581 gacgatattt tgaaggactt gtttatactc acggtaggct attacggtgt cacgattgga
    14641 agcttcttct tttgaaagct ccataaaacg cttggtgtct gaaacgacat cagggtcact
    14701 cagcaattct cctagttctt cataacggtc ttctacagct tgtagttgat catagatgtt
    14761 catttttctt cattctcctt attgatttca ggggcaaaat aatgtttacg acaaaccgag
    14821 atataggttt cattaccacc aatctggatc tgttctccat cgtaaacggg cagtccatcc
    14881 tgtgttcgca acaccatggt cgcctttttc ttgcaatact gacagatggt cttgatttcg
    14941 tcaatcttgt ctgctaaaag caagagatat ttggaacctt cgaacaattc attgcgaaag
    15001 tcatttttca agccaaaagc catgacgggt atgtctaact cgtctacaac acgagctagg
    15061 tcataaacat ggtggcgttt gagaaactgg gcttcatcga ccaaaacaca gtaaggtttt
    15121 tctggtaggt ctcggatata gccaaagata tccgttgttt cctcaatcgc aagggcaggg
    15181 cgtttcatgc caattcgact cgacacatag ccaacaccgt cacgcgtatc cagagccgag
    15241 gtcataatca caacaccttt tccttgctcc tcgtagttat aggccacttt gagaatctca
    15301 atcgttttac cagagttcat ggtcccataa cgatagtaca actgtgccat gtttcttgct
    15361 tcacgtccat ttctaaattt ttgctacatt ctagtatatc ataattttct taagctttaa
    15421 acggcaaaat gtggtaaaat ggaagaaatc aaaactagtg gaggaagcta ttatgccatt
    15481 tgtacgcatc gatttatttg aaggacgcac gctcgagcaa aagaaagctc ttgctaagga
    15541 agtaacggaa gcagttgtcc gcaacactgg agcccctcaa tctgctgtcc atgtcatcat
    15601 caacgacatg ccagaaggaa cttacttccc acaaggggaa atgcgtacta aataagctag
    15661 cttaagcaga attgcttagg ctttttcaat ctccaagtag cattcattga agaaatatcc
    15721 taaatttgtt acaatttgaa aagaaacttg gagaatttcc aagaaaagag ctattaatta
    15781 aaggaaacat tatgattaca cgtgaatttg ataccatcgc tgctatctct actccactag
    15841 gtgaaggggc tattggtatt gtccgcctga gcggaacaga cagttttgct attgcgcaaa
    15901 agatttttaa aggaaaagac ttgaacaagg ttgccagcca cactctcaac tacggtcaca
    15961 ttattgatcc tctgactggt aaagtcatgg acgaggttat ggttggggct atgaagtctc
    16021 caaagacctt cactcgtgag gatattatcg agattaacac ccacggtggg attgcggtga
    16081 ccaatgaaat tctccagcta gctattcgtg aaggggctcg gttggcagaa cctggtgaat
    16141 ttaccaaacg tgctttttta aacggtcgcg tagacttgac acaggcagag gctgtgatgg
    16201 atatcatccg tgccaagact gacaaggcca tgaacattgc ggtcaaacaa ttagacggct
    16261 ccctttctga cctcattaac aatacccgtc aagaaatcct caatacactt gcccaagttg
    16321 aggtcaatat cgactatcct gaatatgatg atgttgagga agctactact gccgttgtcc
    16381 gtgagaagac tatggagttt gagcaattgc taaccaatct tcttagaaca gcccgtcgcg
    16441 gcaaaatcct tcgtgaagga atttcaacag ctatcatcgg acgtcccaac gttgggaaat
    16501 ccagccttct caacaacctc ttgcgtgagg acaaggctat cgtaacagat atcgctggga
    16561 caacacgaga tgtcatcgaa gagtacgtca acatcaatgg tgtacctctc aaattgattg
    16621 atacagccgg tattcgtgaa acagatgaca tagtggaaca aatcggtgtt gagcgttcga
    16681 aaaaagccct caaggaagcc gacttggttc tactagtgct aaatgccagt gaaccactga
    16741 ctgcgcaaga cagacaactt cttgaaatta gccaagatac caatcgcatt attctactta
    16801 ataaaaccga cctgccagaa acgattgaaa cttcgaaact acctgaagac gttatccgta
    16861 tttcagtcct taaaaaccaa aacatcgaca agattgaaga gcgaatcaac aacctcttct
    16921 ttgaaaatgc tggcttggtc gagcaagatg ctacctactt gtcaaacgcc cgtcacattt
    16981 ccctgattga aaaagcagtt gaaagcctac aagccgttaa tcaaggtctt gaactaggga
    17041 tgccagttga cttgcttcaa gttgacttga cccgtacttg ggaaattcta ggagaaatca
    17101 ctggagatgc tgctccagat gaactcatca cccaactctt tagccaattc tgtttaggaa
    17161 aataagaaaa atccatgatc cttcattcgg tcatggattt tattgtcttt attagtaatc
    17221 tggtcttaag acccctgtta cagttgcctt agttgcttcg tagtcgccat ctacgacaac
    17281 cttgataatg cgtttggcat cttcttctgg tgctggaaca agaggtagac gagtgggtcc
    17341 agcttcaaat cccatatagt taagaattgc cttaactgga gcaggacttg gataagagaa
    17401 gagagcatta accttaggaa tgaatttacg ctgaattgct gcggctttct tcatatcgct
    17461 ttctgcaatg gcagtaaaca tctcgtgcat ttcatcccca tttgtatgag aggcaacaga
    17521 aataacccca tccgccccaa ggttcatggc atggaaagca tctccatcct cacctgtata
    17581 aatcaagaac tcttcaggct tgtgctcaat caagtaagcc atattagcca agctagtaca
    17641 ttctttgaca ccgataatat ttggatggtc agccaaacga agcatggttt ctggagtcaa
    17701 ttcgacaact acacgccctg gaatgttata gataataatt ggtaggtcag aagcatctgc
    17761 aatagcctta aagtgctgat acatcccttc ttgagaaggt ttgttgtagt aaggaacaat
    17821 agcaagccca gctgcgaaac caccaaattc cgctacttct ttgacaaact caatagagtc
    17881 acgcgtatca ttagtaccta cacccgcaat caaaggaacg cgtccattga caaccttttg
    17941 tacagccgca aacaactcca actcctcatc gtgggtcaaa gttggactct cagcagtcgt
    18001 tcctgcgaga agaattccat ccgtatgatg ggccaataaa tgctcaatca aggctggaat
    18061 agcatcaaag ttaatggaac catcctcatg gaagggggta ataaaggctg taatgatttt
    18121 acattctttt aaatcttgat aagacacgaa aacacctctc tatttcaaag aaggagttca
    18181 tctgaactcc taaacgatat gactatttta attcaaattt caattcggct gttggacgaa
    18241 ccaatccacg ttcatgaaga gtttcagcaa tctgaactga gttccaagca gcacctttga
    18301 gaaggttatc tgaaacaacc cacatgtgaa ttcctttttc tgcatccaag tctttacgga
    18361 tacgaccaac aaaggtatca cgcgaaccaa ctgcattgat ggcttgagga tagatttgat
    18421 gagctacatc atcttcaaga acagcacctg ggaaggctgc gatagctgct tttacttctt
    18481 cgattggagc cacttctttt gtttcgatat aaacagactc agagtgagct gacaagactg
    18541 gaatacgcac acatgttgca gatactgcaa tgctatcatc ttccataatt ttcttagttt
    18601 ccttggtcat cttcatctct tcgtaagtgt aatcattgtc agtgaagaca tcgatttgtg
    18661 gaagagcatt gaaggcgata ggataatgtt tcttgtcacc gcctgaaggt aagatttccg
    18721 catgcaaatc acgtggtttc actccatcat tcaagacctc acgaagttca cgttgtgtct
    18781 caagaattgc tcccatacca gcacctgaaa cggcttgata agttgaaaca atgatacggt
    18841 ccaagcccca tttttggcga accggttcaa gagccaccat catttggatt gttgaacagt
    18901 tagggcaggc aatgattccg ttgtgggcat caagtgcatg agcattgacc tctggaacaa
    18961 ccaaaggaac atctggattt tgacggaaat aagatgtatt atccactact accacgccag
    19021 cttttactgc gtatggtgca tacttagctg atgtcgaacc acctgctgaa aagagagcaa
    19081 tatcaactcc ttcaaaagct gtttcagtcg tttcttcaat cgtaatatct tgatctttaa
    19141 atttcaatga cttacctgct gaacgtgcag aagcaaggta acggatttta tcgattggaa
    19201 gtgttgattc ttccaacatt tttatcatct gagcaccgac agcacctgtc gcgccgacta
    19261 cagcaactgt atatcccata aataacctct ttcggaattt tctaaaaatt tctataatag
    19321 aagtattata ctactttttc catgaattgc aaagcttttt catcattttt tggaaaataa
    19381 aaatcccagt cgctagacta gggattaaga ggcatcttca tgtgatgagc aggttcacac
    19441 aactcatcaa ggtccgctcc tgcgttatga cctccttatg ctcaatagta gcccgaagac
    19501 tacctatcga ctcatcgcat ctactattct actaaagaga atgacttttg tcaacagacg
    19561 aaactcttta tactcttcga aaatctcttc aaacttcgtc agcgtcgcct tgccgtatgt
    19621 atggttactg acttcgtcag tttcatctac aacctcaaaa acatgtttta agctgacttc
    19681 gtcagttcta tctacaacct caaagcagtg ctttgagcaa cctgcggcta gtttcctagt
    19741 ttactctttg attttcattg agtattagtt ttatttacac aggtgcataa gaaatatatt
    19801 ggaaaaattg ggggcagaca taacaaaaac ccttgtaaat caagggcttt tctgtctatt
    19861 taatgctaat tgccgggatt gaaccggcga cctcatcctt accatggatg cgctctgcca
    19921 actgagctaa atcagcttac ttaagaagta tactatattc agaaaaactt gtcaagtttc
    19981 cttagaaatt ttccatagga aaaagccagg taagaactac ctagcttatc ttcttctatt
    20041 tacttaaatc aattcgacga ccaattttac caataataat cgctccaata atcaatgaag
    20101 caaattcacc gattcctgtt gtaaaccaag taaggaagaa tggtaattct gctacaatat
    20161 ttaactctgc agcgatagta aacatggaaa ttgaaaagag gattgaaaag aagaaatgat
    20221 cttttcgaat caatccgtta aagaggtaat ccttgctata ttttgcaaaa agccaaacac
    20281 ctaaactgag gaatactaag gttgaaccac caccaacaaa gacatctagc agtccgaagc
    20341 taaagaaatt agcaatcata caaccgatgg taactccgat gatgtactta ggattgtaaa
    20401 aagccataaa gttcatcatt tctgaaatac gaaactgata agcaccatag ctgatggcat
    20461 ttagaggcgg tgtgacagtc aaaacgacgt agatagcagc gacgattgca atatctgcaa
    20521 catcacgaat agttaatttt ttcatctttt ctcctttggc ggttgcccgc gtgtaatatg
    20581 cttggtgaaa gaagctaagc accaagggtt gctttattca accctactag tatagcacaa
    20641 tagcctgctt tatgctatac ttaacacatg aaaattccta ttcaaaaatt ttttaacaat
    20701 gaaatcttag cctatctctt ttttggcctt gcgacaactc tagtttcgat tctctcacga
    20761 ctagtcattt atcaactaag tcatcaggaa ctcctggcta ctgccctagc aaatattatc
    20821 ggtatcctat ttgcctttat cacaaatgat acgattgtat ttaagcaaaa aaagaaaaat
    20881 agacttatcc gtttagtgaa attttctctt gctcgccttt ctacttttct gttagacttg
    20941 tttttaactt ttctctttgt aacccaattt cctcacatca tagggcaatt cgtaactgaa
    21001 aacattgata agataaatgc tattgaaaca ataattgcac aaatactcat tattatcatc
    21061 aattatatct tgagcaagat ctatattttt agaaaataat gctccattta tgagcctttt
    21121 aattgccttc tataatgatt taggatacaa tgaaacacga aacattgaaa ggaaaacagt
    21181 atgttaaaaa atctaaaatc gttcttgctt cgaggaaatg ttattgacct tgctgtcggt
    21241 gttgtaattg cctctgcttt tggtgctatc gttacttcac ttgtaaacga cattatcact
    21301 cctcttattt taaatcccgc tttgaaagct gctaaagttg aacgtatcgc tcaactttct
    21361 tggcatggag tcggctatgg taacttctta agtgctatta tcaattttat ctttgtgggt
    21421 accgccctct tctttattat caagggcatt gaaaaagcac agaaactgac tggcataaag
    21481 gaagaaaaaa ctgccgaaaa aaaaccaacc gaattggaag tccttcaaga aataaaagct
    21541 ctccttgaga aaaaataatc gattaaaagg atctagcgca aagctcaatg tcattaagtt
    21601 agtgatctaa ctacttgaat aggaggtgtg attgggatca tcaaagtgat tctctatcac
    21661 atc
//
LOCUS       DAVKDZ010000026        21565 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000026 DAVKDZ010000000
VERSION     DAVKDZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.027"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(1..819)
                     /locus_tag="VWZ30_001029"
                     /pseudo
     CDS             complement(1..819)
                     /locus_tag="VWZ30_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1338..3065)
                     /gene="ezrA"
                     /locus_tag="VWZ30_001030"
     CDS             complement(1338..3065)
                     /gene="ezrA"
                     /locus_tag="VWZ30_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEU9334789.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVEEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(3147..5093)
                     /gene="gyrB"
                     /locus_tag="VWZ30_001031"
     CDS             complement(3147..5093)
                     /gene="gyrB"
                     /locus_tag="VWZ30_001031"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU9334790.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEIVGDTDKTGTTVHFTPDPKIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKREG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSESRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(5108..5671)
                     /locus_tag="VWZ30_001032"
     CDS             complement(5108..5671)
                     /locus_tag="VWZ30_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9334791.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDISNKGGHHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(5756..6310)
                     /locus_tag="VWZ30_001033"
     CDS             complement(5756..6310)
                     /locus_tag="VWZ30_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU9334792.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLSAICAA
                     PIALNQAEILKNKRYTCYDGVQEQIFDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(6321..7544)
                     /locus_tag="VWZ30_001034"
     CDS             complement(6321..7544)
                     /locus_tag="VWZ30_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9334793.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(7581..10031)
                     /locus_tag="VWZ30_001035"
     CDS             complement(7581..10031)
                     /locus_tag="VWZ30_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEU9334794.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDYYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEEIYRNQSILTSPDLVQVQGLYF
                     KKTAASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPHNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(10231..11160)
                     /locus_tag="VWZ30_001036"
     CDS             complement(10231..11160)
                     /locus_tag="VWZ30_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9334795.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            11300..11587
                     /locus_tag="VWZ30_001037"
     CDS             11300..11587
                     /locus_tag="VWZ30_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEU9334796.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(11621..12955)
                     /gene="ciaH"
                     /locus_tag="VWZ30_001038"
     CDS             complement(11621..12955)
                     /gene="ciaH"
                     /locus_tag="VWZ30_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEU9334797.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12945..13619)
                     /gene="ciaR"
                     /locus_tag="VWZ30_001039"
     CDS             complement(12945..13619)
                     /gene="ciaR"
                     /locus_tag="VWZ30_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEU9334798.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(13728..16274)
                     /locus_tag="VWZ30_001040"
     CDS             complement(13728..16274)
                     /locus_tag="VWZ30_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU9334799.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEITARVDLISREKAAVEAVVLQYGNA"
     gene            complement(16397..16837)
                     /locus_tag="VWZ30_001041"
     CDS             complement(16397..16837)
                     /locus_tag="VWZ30_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU9334800.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVKPFNRVSADHAYKEGE
                     GNKTLVYWRQVHEDFFRDCLGEAGLTFTPESKVVLEEFRKVYPL"
     gene            complement(16812..17681)
                     /locus_tag="VWZ30_001042"
     CDS             complement(16812..17681)
                     /locus_tag="VWZ30_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEU9334801.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTKVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(17659..18159)
                     /locus_tag="VWZ30_001043"
     CDS             complement(17659..18159)
                     /locus_tag="VWZ30_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9334802.1"
                     /translation="MEIKKHFGVYAVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETRFTVRSYSNPRIYDVFVREELKNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(18170..18868)
                     /locus_tag="VWZ30_001044"
     CDS             complement(18170..18868)
                     /locus_tag="VWZ30_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU9334803.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18861..19127)
                     /locus_tag="VWZ30_001045"
     CDS             complement(18861..19127)
                     /locus_tag="VWZ30_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEU9334804.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(19195..20121)
                     /locus_tag="VWZ30_001046"
     CDS             complement(19195..20121)
                     /locus_tag="VWZ30_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEU9334805.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSQAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(20196..21011)
                     /locus_tag="VWZ30_001047"
     CDS             complement(20196..21011)
                     /locus_tag="VWZ30_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334806.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWENFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILILAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRWCLVQDSPKMKIGTYI
                     VVGILVLLVVMGYVGIGSFVQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(21015..21335)
                     /locus_tag="VWZ30_001048"
     CDS             complement(21015..21335)
                     /locus_tag="VWZ30_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9334807.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSLDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(21425..>21565)
                     /locus_tag="VWZ30_001049"
     CDS             complement(21425..>21565)
                     /locus_tag="VWZ30_001049"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334808.1"
                     /translation="VFEVVDETDEVSSKHCFEVVDETDEVSNHTYGRATLTWFEEIFE
                     EY"
BASE COUNT         6487 a         4714 c         3828 g         6536 t
ORIGIN      
        1 tttaaggtac ttgatttatg ccatatagca ttgtccataa cgagtaaaag ataatcatct
       61 ggataagctt gtgaaagctc ttctaaaaag gcgttcatcc actcagtatt acatccacca
      121 gctattaaga aaaatgattc gcctgtatgg gcatcaacag ctccataaca atagcgaaat
      181 tctcgtatat agtgactatg gatatgtgga cctactccta ttggagccca acaagatccc
      241 agtttactga ttctaccgaa accagcctca tcttggtaca tcaagcgaac cttacgaaaa
      301 cgtctactgg ttttaaagcg ctttctcgtc ttcttgaatt gagattttat ttttagacgc
      361 gacaatggtt tgagcgtctg ctttcctaag atgttctgga cgtggcataa tatttcgcca
      421 accatggcgc ttcaacagtt gatagaaggc atcacgtgtg taggaacgac ctaactcctt
      481 tttataagcc tgaaataagg catcaattgt aacaaattct cctgcctctg tagccttcaa
      541 atggcgggca agaaaggctt tctcttcctc aactgtcata tatgcatggt tacgaccacc
      601 acgtgtttct tgaaggagag agtcgagtcc gaactcctca tattttttta catttcgcca
      661 aatcattgtt tgattacagt ctaaaagttc tataatctct ttataagatt tgcccatcag
      721 acgaaataga acgatttgaa ggcgtttatg atatttagct gtacgagagt cttttaaaag
      781 tgttttgatg gtttggattt cttctttaat tgatttcata ttactataat gtcttccaaa
      841 cgaggaaact ctcgtaaaca aagaggtttt agaggcctat ttaccgtgga ctaaagttgt
      901 acaagaaaag tgcaaataag aaatctccag attaggaact atccgtgagt tctctaatct
      961 gaagattttt caatagactt cgttattgga cggttaccaa gttactcaaa gtacagattt
     1021 gaagctgcag ataagactga cgaaatcact ttaatactaa atgaaaatca aaaagcaaac
     1081 taagaagcta gcctcaggct gctcaaagta cagctttgag gttgcagata aaactgacga
     1141 agtcagtaac atacgcacgg taaggcgacg ctgacgtgat ttgaagagat tttcgaagag
     1201 tataaaacac tgttttgagg tggtggataa atcggctggg caaaaactca atctcaggag
     1261 aaaatgaagt aaatctcccc acaactatag aaaaagattt gattctgctc ctcacacaat
     1321 aaaatctttt tcttttatta aaaacgaatc gtttcacgtg ttttctcata tgaggtaaca
     1381 aagcgattgg ttacaccagg ctcttccact tccaatgctt gagaaatctt gtcaaatgaa
     1441 gcgtgataat caaattcttt ttcaaaaata tctaaagctt cgttaaatgc ttcttgaatg
     1501 cgttcatcaa atgagcgata gcggttagaa tattgcaaga gttgctctgt caaagttgca
     1561 tattgtacaa tattataagt ttccgtttct aaagcttcca tatcattcgt tgcaatttca
     1621 agaactcggg taacagattc aatgttaatc attttttgtt ctaactcaac cattaaatcc
     1681 tcggtattat tgcttgccgt aaagaataac ttcaagaaag tttgtggaat acctggcaga
     1741 ttgcgttttt ccatgtatcg cttgatagta tggagacgat tgacataaac attggccttt
     1801 tgacgtgcat taatatcatc tttctcaatt tgtgtcaggc gctcactaac tgaaatttgc
     1861 tcatcttcaa tatcttttag ttgagtttgt aaatcctcaa gattttcttc aagaactgaa
     1921 taagcttggg ttggttcttc ttgatttgaa gttacctcaa caatggctgc ctcaaaactc
     1981 tctaattctg tctgaatacg acgaacatgg cttgcagctg tctcaggaag taaataggtc
     2041 ttgttcaaac gtgcaatatc ttctcccaat aaagtattat tctctttcat atgttgaagg
     2101 taagttggaa gagttgcaag tagattttcc actactttct gagcagcaat ttctcgagta
     2161 aaaatatcat acaaggcatt gatttcctct tgtgcctgtc cattctcata ttcggcatta
     2221 tccaattcca actgacgaat attctcttgg tttttcttga atgcttcata aagcaagtgg
     2281 aaacgcgctt caatatccgt ttcaacaaaa tgataattag catcaattag tttacgataa
     2341 ccggcttcca aatcctgtaa ttgatctggc aattctgtag aaagcgtcgt aaccaaggct
     2401 ggaacacgat ccacaatatg acttaaggcc aaaatgtgat tttctgtatt atccaaaatc
     2461 actgcggctt ccacagggtc acccgatgaa ttcaaggtta caaattgtga aaattcagat
     2521 tggatatttt ctaattgttt ttcaatttca tccaaggctt gaccatactg ttctgaattt
     2581 tcagcaactc tatgctgaag ttcctcaaat aaatccaaag catgaagaac acgaccacta
     2641 tttttagatt cttgcttctc taagtctgcc aaagcattgc gaattgccgc aatatcttct
     2701 tcaatcaaag taatttgact ctcaatttgg tcaatttgat gactggcctt gagaaaacga
     2761 aatgaatggt tatagccttc tgcttcaaag agattatttt caatatcggc aaaagagttg
     2821 agagataaat cgacccattt ttgattccat tcacggaaag ccacttgact ttgtccaatc
     2881 aagtgcatat tttttacagc ttctacttca tcatttactg gaagattgta tagttcttct
     2941 tttctttctt ctagcgcctc taatctcccc tcgtttcgct tacgtagaaa gattgccact
     3001 acataagcca gaactaaaat gactgcaatt gcaaccatta aataaattag ttgtccatta
     3061 gacatatcaa actccttttt tacttgaaac aatcgtaacg attatatcat attttttaga
     3121 ccaagggaac tacttctccc tattttttag acatcaagtg tactatagac agcattttct
     3181 tcgataaact cacgacgagg ctctactcga tcccccatca acatatcaaa gattttatcc
     3241 gcttctgcag catcatctac agaaactcta gccatcaagc gatgttcggg atccatggtt
     3301 gtttcccaca gctgatgatc gtccatttca cctagcccct tataacgctg aatagtcggt
     3361 ttggtacgac tttcactata acgggctaaa gcttcttgga gtttgatttc ttgatctgca
     3421 cccggctgga tatattcttt aatctcgctt ccaaccttga caccatagat tggtggctgg
     3481 gcaatataaa cataaccagc ttctaggatt ggtttcatat aacgataaat caaggttaaa
     3541 agaagggtac gaatgtgggc tccatcgaca tcggcatcgg tcatcaaaac gagtttttgg
     3601 taacgggctt tcgaaacatc aaattctgcg ccaaatcctg ttcccatggc tgtgaaaaga
     3661 ctacgaattt cttcgttagc tagaatctta tccatacttg ctttttcaac gttcaaaatc
     3721 ttaccgcgaa ttggaaggat agcctgaaac tcacggttac gaccagattt ggctgatcca
     3781 ccagctgagt ctccttcgac gatgaagagt tctgtttcag cagggttatt agaagaacag
     3841 tctgctagtt tccctggaag gttggaaatt tccaaaccag attttttacg tgtgacttca
     3901 cgcgcacgct tggcagccac acgagccttg gcagccaaaa ttcctttttc tacgatacgt
     3961 ttggcaatct gtggattttc catgaggaaa tcggagaagg cttcactgaa gaggcgattg
     4021 gtaatcttga ccacttcgct atttcccaat ttggtcttgg tttgtccttc aaactgtgga
     4081 tttgggtgtt taactgagat aactgcagtt aagccttcgc gaacatcttc ccctgttagg
     4141 ttgtcttcat tgtctttcag taacttattt ttacgagcat aatcgttgat aacacgtgtc
     4201 aaggctgtac ggaaaccttg ttcatgcgtt ccaccttcat gtgtatgaat attattggcg
     4261 aaactcatga cattttcatg gtaacccgtt gtgtactgca tggctacctc aactgtgata
     4321 tcatccatct caccgtctgt atagattggt gtatcaaaga ttacatcctt gttctcgttg
     4381 atatattcaa cgtaactggc aatcccacct tcataatgat aatgcttggt ttgttccaaa
     4441 ccttcacgct tatctgtaat tgaaatttga agaccgcgat ttagaaaggc caactcttga
     4501 atccgtttat ttaatttatc aaaatcaaag attgttgttt cagtgaagat ttttgggtcc
     4561 ggtgtgaagt gaacagttgt tcctgtttta tccgtatctc caactatttc aagatctgcg
     4621 acaacatgac cacgacggta ttcttggtaa tgaatcttac catttttgtg aacatgaacg
     4681 tctaattgag ttgaaagggc attaacaact gacgacccca ccccgtgaag accacctgaa
     4741 accttgtatc caccaccgcc gaactttcct ccagcgtgaa ggactgtaaa gacggtctca
     4801 acagcaggac ggcctgtttt ttcctgaata tcgactggga taccacgccc atcatccaca
     4861 acagtaatcg aatcatctgg ctcaataaaa acttgaatat ggctggcaaa tcctgccaag
     4921 gcctcgtcaa ttgagttatc aacaatttcc cagactagat ggtgaagacc ttcttttgag
     4981 gttgatccaa tgtacatccc tggacgcata cgaacagcct ctaagccctc taaaacttga
     5041 atttgactgg catcataatc ctgtgcctgc agatttttga tttcttctgt catcttattc
     5101 ctttttctta tatgtctaat acttgtctta aattcacgat actggtaaac aagtgggtat
     5161 caagtcctgc agcttgacct gcttcgatat caatcggccg atcaccaatg acaagaccag
     5221 agctaatctg atacttttct cttaaataaa gcatggattc gggattcggc tttctcttaa
     5281 agcctgagct agaagtcacc acttctgtaa aataagctgc tatagaggtt ttttctaaaa
     5341 tttccaaaac ctgatcattt cgatgagaga ccaaaaaatg atggccacct ttatttgaaa
     5401 tgtcttccaa taggtcagaa actccttcaa ataaaatcgg gtgttcaagc tctctggctt
     5461 cattttcctt gtacttttct aaaaaattct ctaaattggg agcgaatgtc tcaatcgcaa
     5521 aaggagtaga aacctttaaa gcttgataga cactgtcatg gtcttgtgtg ataccataca
     5581 gtgccaatgt ttcaacaaat gcagctgttg aagtttcata attatccagt aaagttccac
     5641 ctaaatccca gatgtaatcg tgatatttca tacccttcat tatagcattt ttttatgaaa
     5701 aaagcgatga tttccctgag ttttccacat aaaaaacgag agaataactc ccgttttact
     5761 gatttttacc aaagacatct cgatagagca ttcctgttcg taaactctct gcgtcccctc
     5821 ctagttgctc caccaactcg taggcaaagg caagggctgt tgaaggaccc cgactggttg
     5881 tcaactgacc atctaccact actgtttcct tgacgtagtg accatcaaag atttgctctt
     5941 gaacgccgtc ataacaagtg tatcgcttat ttttcaatat ctctgcttga ttgagggcaa
     6001 ttggtgccgc acaaatggct gatagtttct tcccttcttg ctcgaagctt tgcaattctt
     6061 gaatcaaggt ctgattatca cgtaaatgtg cagaaccagg catacctcca ggaagaacaa
     6121 tcatatcata gtctgataaa tctccatcaa agacatgatc tgctcttact tggattgcat
     6181 gcgaacccgt tacttgctct tcaaaaccaa ccatatcaca tgtgatattg gctcgacgca
     6241 agacatctac aactgtcaag gcttcaattt cttcaaagcc ctgagctaac ataacggcta
     6301 cttttaccat gattttctcc ttatttaatt tgttttaata caaccttttt ccgtttgaat
     6361 gggacttttc cgctcttttc ttcagctaaa ttagtctggt aactcatcga taaaagcaaa
     6421 ccaacaccaa tcaaattact gataatagct gatccccctt gcgaaatgaa aggcaaggga
     6481 atccccgtca aaggaagtag tccagtcaca gcaccgatat tctcaaagat gtggaagagc
     6541 aacatcataa tcaaacctgt ggaaatataa gtgtagaact ggttatttga tttaagagta
     6601 atcttcaaca tacggtaaat caacatgaga tagagggcaa taaccaggac agagccaata
     6661 aagccaaaat cttctgcaat aaccgtaaaa atcatatctg actctcgaac tgggataagc
     6721 agattcgaag cattaaatcc ctgaccaaat aagccaccac tcccaatggc aatctgccct
     6781 tgagcctgct ggtaagtcgt tgtttgggca aactcaaagg gattgagcca agccaaaatc
     6841 cgattaattt ggtaggtcgg cattccaatt tggtgaagaa aagctcgtcc gtccttgcta
     6901 ataaagatag ctaagaaacc agcaactcct gttacagcag tcacaaatac tgggataata
     6961 attttccaag aaacccctga taataaaacg attcctgaga aaatggctac aaaaaccaaa
     7021 gccgtcccca agtcactttg aagtgctaaa agaactagga ctggaatggt aaagagaatc
     7081 atccagaaaa ttaacaaaaa gtccagcgga accgtgcgtc tccattcctt atgtttcttt
     7141 gtaaattgga caatgacacg agccaacatg aggatatagg atatcttcat aaattctgac
     7201 ggttgaaata gggtaattcc atttattgat acccagtttt tggcacccgt tgatgcaact
     7261 aagcttggat tataaaatac aatcggcaag atcataagtc ccaagcctaa aatatataga
     7321 aagggggtca ccttccaaag aaattctgta ttaaagagca tgaccacaaa accaatcaca
     7381 agccccaagg caatccaggc gacctgctgc cctaaaatgg gcagaatatt attgggataa
     7441 tcatgactaa cggctatata gatagccacc acaccgatga ccagtagaaa aaatactggc
     7501 aagagcaaac tataatcgac tctagagtcg agagaacgtt tcatatatac taaccttata
     7561 ctttcataca atactattta tcaaagttca ttaaaaaatc tatcaatagc ctcgtcaact
     7621 tcggatcgag agatggtttt aacagtcgct tcttctgcta gagatgctac tatttgtttg
     7681 ccatatcgtt ttccgacgat tctcctatcc aaaataagag ttaaggaacg ttggtattca
     7741 cgtctcatac ttcttcccaa agcctgtttt aaacgaataa tggccattgg caattgataa
     7801 tcataaaagg catttttccc ttcttgattc agttcttgat taatcttttt cgtcaagggt
     7861 tcttgaggat tttggaaagg aagcctcggt acaacttgaa tcacaaaagg atggcttgaa
     7921 aaatcaactc cctcccagaa acttgctgca ccaagcaaga tttgttgttc acctttttca
     7981 aagcgtttct ttagctgatg aacatcccca tttttatact gggccaagtg gctaactgta
     8041 agtaaatccg atactgctag aagcatgtct ttagcggtaa agagaaccaa aatcggttgc
     8101 tggaaagctt gaatttccac tagtaaagca gctacctctc tggcatagac ttctaaggag
     8161 gtttctgtta ccagagggaa atctttgacc atgaccactt cttgttcctg ttttccccga
     8221 gattcaatct tgacaaattt ggcttcagga tagcctaaaa ggtctgccaa agaaaccctc
     8281 tgactaatct caagagtagc cgatactccc aagacttggc aactctcagg aagtaaagag
     8341 gacagaagag tacggcctga ttttgtagaa gaaatctgaa ttttcttttg actcgtttca
     8401 gttacttcaa gccagtaatc accttcagct gtaaaatagc gaaccagctc atcaaagcct
     8461 tctacttcta attctgaaaa atactgatgg agattgtcca gagaatctaa gatatttttc
     8521 ctagatttac cagactgaaa ttgttctatc aagtagagac actcaaagcg aatactttct
     8581 agtatccgtt gttgaaccct gttttcttct cctactaaag ccttatcaat taaatcgata
     8641 atagattgta tatcgtaggt ctcttgaagc agattttcta gagctaacaa aatcttttgg
     8701 acttcatcaa taatcagtaa acggtcactg acaaattcag gattatcttc aagtctggtt
     8761 acgagatagg catgattagt cactaaaagc ttgcaagtct ctgccctttc ttgactacgt
     8821 ttccaaaaat cttccgtcac aaataagctc tgggatgata aattcccatc atgacgaagg
     8881 tctgctagaa aatgttggta acggtagagt tgcccgattt catccaaatc tcctgtctct
     8941 gtctcagtaa gccagaccaa gacttgcatt ttaaagcgtc taaataagcg attttcatca
     9001 ttttcctgca aggaatgata aaaggcatcc aacttcagat aattgtgtgg tccctttaag
     9061 ctatgaatat ctgtatggaa cacttccttg agacgtttac cttcttcttc catgatttga
     9121 ttttgaagaa tctttgtcgg aacactaaga acaatttgtc gctctttgga ttgagataaa
     9181 gcgggtaaga gatagccata ggttttccca atccctgtcg gcgcttgaat cagagagaca
     9241 ggttcatctt tcaatagcaa gccaacctct ttagcaaaac tttcttgttc ctccctcact
     9301 tcaaggttca acagagaaat atttttagaa aagtcttgag atagttttcg tggctccaga
     9361 gaagctgccg ttttcttaaa atatagacct tgaacttgga ccaagtctgg agaagtcagg
     9421 atagattggt tgcgataaat ttcctcaata accaggtagg actcatatag gagagcgtca
     9481 gccatttcca gcaagcgttc caagagacct ttaggaagct gggtcatctt ttcccgtaaa
     9541 aaaagaagta attctgctgt agcttgggca tctgaaaggg ctgtgtgtgc gtgtttaaga
     9601 ggaattccta attctcgaca caaaatcggc aagctatatt tttccagttc agggaaaaag
     9661 acctgggcca attcgaccgt atcaacacga gggtttctta gctcatagcc ttcaaaaaat
     9721 aaattttccg ccaagagatt agcatcaaac tgaacattat gggctacaaa aatcccatcc
     9781 tccaccaagt caaagatttt tctggcaact tgcgaaaaat caggtgcttg cgccagacgt
     9841 tggtctgtca atcctgtcag ttctttgata tgagcatcca agggttcatg tggattgaca
     9901 tccgtcgtat agtaatcgac gatttctcca tcctcaatca cgacaattcc cacttggata
     9961 attttagcct tactacctgt gctagttgcc tctaaatcca ccacaacata gcgattacca
    10021 atcgttttca ttataaaaaa ttataccaaa aaaagggcca tttggcacct gcaaacatag
    10081 gataattttc aaactcaaga acactacttt tcttaaaaat cccatcgaat agagcattct
    10141 agcctgctga tttttttaaa atagtgaaga aatacgaaaa attgagagaa gaaatgtttg
    10201 catcttcctt ctctcaacga atcatgtatt ttatttaacc atgtcaggat cgtagtcata
    10261 aaatcctgta tcaaggaaac ggtttttagg gtgaagttta cgaacttctt catccagcaa
    10321 gaaggcttgg tcatggctaa agttgccctc ttgaatcaag ctacgcgctt ggataaagag
    10381 ttttgcaatc tcaacaaagt tctgaccagg agtgtagagc ccttctagtt tccagcgagt
    10441 aaagccatgc tccaccaatt ctgttaattt gatcatcaaa tcaaggtcat tgttggcaaa
    10501 gatatgggtc ccatgattat cttcaaaaat ggaatagtgg ctctctggat cacttggctc
    10561 agccaagaag aggtcacgtt tatgcgtctt ttcatcatcg atatgtgtaa agttatagta
    10621 gttttgcaag agcggacgtt tagaatgatg gatgacacta gcaccgtaaa ccaaaacttc
    10681 agcaggaatt tccaaaatct ctggcatttt gaaaagttca gctgatggaa tttcacgcgc
    10741 caaaacagcc tcagatgcgc cagccttttg tccccagaag ttaatctgac gactgctagt
    10801 taccatggtt gaagcatcgt agatggtctt aaatgaataa ccatcgcggt tgactacgta
    10861 aaagacgcct gcatccccaa tcgtaatata gtctgtcttg atttcttcca agaagtctaa
    10921 gaaaggcttg atacggtcca tcatatcttg gtgcatgaga gcattgaccg caacgatcaa
    10981 ttccttacca gcatcatgaa ccaacttagc gatttcacgt aattggtcat aactaaaggt
    11041 cgttggcaga cgaaggccaa aatctttctc accgacatag atacggtcta cgccagcttc
    11101 gagtagttgt tcaacttgtt caatactttc agcagttgct gtaatgacaa tcttttccat
    11161 aagaaaaatt ataccacatt tctccaaaat cagccatgct ttgaaaatga aaagggactt
    11221 tattcttttt gtaacctttc tgtaataatt ctctgccgaa aaaatgataa aataggtatg
    11281 tactgttaag gagagaatca tgcccgtaag aaaattacaa tcctatgagg tagactatca
    11341 agaagaatta aaccagcagc ttcctcatta ccaagcttat acacctgaag cgcaatctga
    11401 tgccaatctc aaggaaatcc tattttttat taatatcgct gttttttgta tctgtattgc
    11461 tatctttagt tttatctttt tagcattaaa attatcaact gctcttgcct ttgccgcagc
    11521 aatcggattc agcttacttg ttttaaaagt tcaacggtct attatcaaac gaaaacgtag
    11581 aagataaatc caaaatatcg ccccaattgg agcgatattt ttattttttc tttttagatg
    11641 gtgtctgaat ggcaatcttc acttcaaaga ttgttccctt gggtttatta tctttgacag
    11701 taacagttcc ttttagagca tctacaattt gcttggctag ggataatcct aaaccaaaac
    11761 cacctttttg ccgggttcta gctttgtcta ctcgataaaa acggtcaaaa attttctttt
    11821 tatcttctgt cgaaataccg attccattat cagaaacaag taaataaaga ttgcgatcgg
    11881 tcgccgagat aagaaaatca atttcaccat cctcctcagt atacttgacg gcattatcga
    11941 aaagaatggt catcagttgt ttcagaagaa gctgatctgt gacaattgtt cgatggatac
    12001 gattttcaaa acggaagaca cgattatttt ccgaagcaat catctcgtag tttgtgaaag
    12061 ttgtattaaa aaagctagtt ggaacttctg caagctccgg cttaatccca tcatctctcc
    12121 gagctaagtt cagcaagctt gtcgttaaaa aacgcatatt tcggacttct tccaaactcg
    12181 atgcaatgct ttcgctcaca tccataatgg tagcttctgg cttacgaaaa agggtctcta
    12241 agcgattttg caaaactgcg agtggagttc gtaactcatg actggcattt tccacaaaag
    12301 actgttgctt ctgcatactc tcaagcaggg gcctaacact gaccctagct agatagagac
    12361 tggcaagtaa agacaaaatc cagaaactag ccatcacgac cacaatcaat tgctcatgct
    12421 tttgactagc ctgctccaac tgactggtat taatcaagac agcagcatac tttatattgg
    12481 ttgaaaccga actaatattg gtctccatca aaatcacacg atagatttct tcctgcccat
    12541 agctattaaa aacctgaatc tggtaaatat gtcctagttc tttcttttct aacttaatct
    12601 tatccaagcc caaaaatcga tttccagaaa gaagttgagt aaagtcttta tcaaagagaa
    12661 tgacttccgt attggaactg acattaggtt ttatttctac tttactagcg tccgccctag
    12721 cattttctaa atctttaatc tcttctgttg ccctatttat agccagctga ataactgctt
    12781 gaggattttc actcagtcca tgaagcttat cgtccaccga agtataaaga ctcgaatgca
    12841 tgacttgtaa aataatcaga gtcattgtag aaaaaatcag ggtgaagaca ccgaagttgc
    12901 ggataaaata actaaagtca tccgcatacc atgttttttt aagtttactg aacatctttt
    12961 aaaagatacc caacactacg caaggtttgc aaattctctg caaaagtggt tccctttaat
    13021 ttcttacgga cttttgaaac atagacttcg acaaccgaaa tcgttgtatc actatcaaat
    13081 ccccatagac ggtcaaaaat ctgcgtctta ggcaaaatca cattttgatt ttgaaggaaa
    13141 taaactagta aatcgaactc tttccccagc aattcgacag gagtatcttc aactttaacg
    13201 gtattggttg ataaattaac cacgatattc ccataagtca aggtgttttc attaaacttc
    13261 cctgaacgtt tgagaagggc ctgaatccgc attttaagtt cttctaggta gaaaggtttg
    13321 gtcagataat catccgctcc cagttcaaat ccatgtccct tgtcatccaa actttccttg
    13381 gcagtcataa tcagaactgg tgtcgtaatt cccttttcac gcaattcttt taagacttgg
    13441 aaaccatttt tttctggcaa catcaaatcc agcaaaatca agtcatagac accactctca
    13501 gcttcgtaga gaccttcttc tccatcaaat acctgcataa catccgcaaa atcgtctaaa
    13561 aagtcaaata ctgaatttga cagacctagg tcatcctcaa ccaataagat ttttatcatg
    13621 agaaactcct ccttattaaa actattatac caaatttgcc ttaaaaaaaa ctcaactctc
    13681 tgcattttac atgagatagc tgagttttct ttttatttta ggcttattta tgcatttccg
    13741 tattgaagaa caactgcttc gactgcagct ttttcacggc taatcaagtc aacacgtgct
    13801 gtaatttcct tgattcccat accgatgtta cggctaagag caaggtcaga aagttgcggc
    13861 tcaaagaact ccttgtattc cgccaagcgt tgctgagtct taaatacatg agcaggaagg
    13921 ataacaaagc tatcaaagct catatctcct ccaagggctg ccttaatcca agcccagttt
    13981 tcacgcgccc aagaccaagc tgttttctga gttgcttgat gagctaggaa ttggtaatac
    14041 caagcagaca agtcctgtgg tttgaccaca aatttgtcct tccaagaagt aatcaagttt
    14101 tggatattat ccgcatctgt actgtatgca agagctgctg ccaactggcg tttaaagaca
    14161 gcatctgttg cgtgagtata agtatcaaga taaagtgcta acaagtcttt agtctcatga
    14221 tgtttcatct cattaatcag aacttgtgag cgaatagctg ctgggagtcc tgcaagattc
    14281 tccttgtgtg ttgcgaagat ttggctagcg acttgactag cttctgcatc atttgagcga
    14341 atcatcatcg aaacagccag ctgacgaacc aattcatcct catctgattc tccgtcttta
    14401 gcttcaaaac caagacggtc atagttatga cgagccaatt tagcaaccag tcctttgaag
    14461 gctgtttcag catccgttcc ttcatcaata aagcgctcaa gggctgaaat cacttgagaa
    14521 acagctgaaa ccaccagata agactcttcc ttagcaagtt tatcaagaac tggaagcaag
    14581 tctgcataag aaatgtgccc tgcctcagcc aacaaacgac gttcttgaac aatttgcagt
    14641 ttgcttgtgt tatcaagtgt ctctagctca gcaagaacag cttctaacaa gtctccttga
    14701 tagtcggtaa tatagtgggc agtattttca gtgttgagac gaagagctcc ttcattttca
    14761 gcaagaagag ctgcgtagcc agggatttcg atactttcag tttcgagtgt atcaggcaag
    14821 cctttccagt tgctattgag gggcaccacc cagagacggt tcttgtcttc gttctcaccg
    14881 atgaagaatt gtttttgtga aatcttcaag acatcatttt caactttgac agtaagaact
    14941 gggtaaccag gctgttccaa ccaagaatcc atgaaggctg cgacatcacg tcctgacgct
    15001 tgaccaaggg catcccaaag gtcactacca atggtgttgc tgtattggtg tttttcaaag
    15061 taggcatgca aacctttagc aaaatcagca tctcctagcc aacggcgaag catgtgcatg
    15121 agacggcttc ctttggcata gacgatagcg ccgtcaaaga gtgtattgat ttcatctgga
    15181 tgtttaactt cgacgtggac agactgaacg ccatcagtag cgtcacgttc aagagcaaga
    15241 ggtactccac ctgtttggaa atcttcaaag atattccagc ttggttcgat ggtatccaca
    15301 cagacgtatt ccatcatatt agcgaaactt tcattgagcc aaaggtcatc ccaccatttc
    15361 atagtcacga ggttcccaaa ccattggtga gccaattcat gggccacaac aagggcaact
    15421 tgttgacggc tagcaaatgt agagttctca tcgacaacca agtaaacttc acggtaggtc
    15481 acaagacccc agttttccat agcaccagct gagaagtcag gaagggcgat gtggagagat
    15541 tgaggaattg ggtacttaac tccatagtaa tcttcgtaaa actcgataga gcgaacagcg
    15601 atatccagtg agaaatcaag atttgaaagt ggatgtgctt tggttgagta gacacctacc
    15661 agggtaccat ttttagtttt agcggtcacc ccttgcaaat caccagcaac aaaggccaac
    15721 aagtaagaag acatgcgagg tgttgtctca aacttccaga tacctgtttc cttacggttt
    15781 tcaacatcga tttctggcat gtttgacaag gccaattcac cttctgcttg gtcaaagcga
    15841 agagagaggt caaaagttgc tttggcttca ggctcatcca cacatgggaa agcttcgcgc
    15901 gcaaaatggc tctcgaactg agtagacaag acctccttct tgactccatc aactgtataa
    15961 taagaagggt aaatccctgt catgttgtct gtaattttac cagaaaaggc aagaaccaat
    16021 tcaacttgac cagcctcagc caattcgata tgaagggctt cattgtcatg gtcaactgta
    16081 aatggacgag cttgacctgc aacttctaca gaggtgattt ccaagtcttt ttggtggagg
    16141 gagatgcggt cactctgtgc ttgaccagtg atggtcactt tcccagaaaa agtcttggtc
    16201 tcacgactca aatctaaaaa taaatcataa tgttcaggaa caaattgctt aataaaatgt
    16261 tcaactgctt gcatagtttt ctcctattct aagtttaaga gctgctgctc ttcttcaaac
    16321 aaacttatta tatcatattt tgcctgagaa aaaaggattg gacagcgatt tccaaaactt
    16381 tcttccttct aatagtctac agtgggtaga ccttgcgaaa ttcttctaaa acaaccttgc
    16441 tttcaggtgt aaaagtcaat cctgcttcac ccaagcagtc tctgaaaaaa tcctcatgaa
    16501 cctggcgcca atagacaagt gtcttgtttc cctcaccttc cttataggca tggtcagctg
    16561 acactcgatt gaagggttta acagaaacct ttgtaatttc gacaatgcag acagactgat
    16621 tttgactatc taaaatgaca tcgaaggtcc ctacttgggg aagtggttcg tcttctagta
    16681 catagaggtc ataggctgat gctgttgctg tcttttctcc tttaaacacc aaatccgcta
    16741 aaaggtctgg ttcaactcca aaagcccaag catcgatttc atctccgatc aaaggattga
    16801 tttgcttgta tttattccac atttcttgcg gtatcatggg tactcctttg taatttttta
    16861 ctttcttctt ttatgtgttt aagatgatct ggatggtcaa tctctaaatc aaaaatctct
    16921 ggaatagaac tgtagtggat aatgcacttg atacccaact gattcatttt ttgtatgaaa
    16981 gaagtattca gatagcctgc tacagcaaaa ttaatcttgt tctttcttgc tttatcctgc
    17041 atatctctta gcatatctaa cattattgga ctttccatat catgccattg actgtttctc
    17101 atagtcgcaa aaacaaagga agtcaaatca ttcattccaa ctacaatctt tgaaatgccc
    17161 gtttccagta tactagataa gtcaaaatac gctgacggta attcaatcat cgttccgact
    17221 ttcccagtaa agccatactg acgcaatact gtaatagctt gttttaattg gtcggcatca
    17281 ttgacaaaag gaaagataag agatagattg ggattggttt gataaacttt tgtaacgaca
    17341 tgtgcttcag cctgaaattc atccaaacac gccagtaaac gcctggttcc tctatagcca
    17401 aacaagggat gcccttcgtc aaaaaactct ctagtcccca ctaaacaatt ggcctctgta
    17461 ttcgttaatt cagaaaaacg ataccaaact tcttcatctg agtacaggga gcagatagtc
    17521 tctagataat cttttacaaa ttgctgacaa cttgctaata tgatgttttg attaagctct
    17581 ctcaacaaat attccccacg aatcatgccg acgtggtgaa atagttgagg ataaattttt
    17641 tcaagaattt tttcgccact aagggcaagt tgatttctca tcattcacct tccaattcat
    17701 gtaagaagtc ttgtccagtt ctggaaatcc taataattca gacttaacct tcaagactaa
    17761 tggcgatgca ttttcttctg taatttcttg aatatccatc caaatatatc caagtgaatc
    17821 attcgcacca tcagacacag cttccgaagt cgtaacttga ggtgcactct cattcatttc
    17881 aacatcatac aaggccatga catggtgaac cataaaattt tttaactctt ctctgacgaa
    17941 aacatcgtag attcgaggat tagagtagct tctaacagta aatctcgtct cttccataac
    18001 ttctctagtc agcgtttccg tcagtccttc accaagttgc tgactgcctc caggtagatc
    18061 ataccgatgt tgataagggc ctctcgtttt ttcaatgcaa agtaactttc cattttcaaa
    18121 gcaaacagcg tagactccaa agtgtttttt gatttccatc caactcctcc tacttcaaag
    18181 accagccacc atctattgtc aagatttgtc cttgcatggc gctcgctttt ccacttgcta
    18241 aaaaaagact aagctctgct atttcctctg gctcaatcca gcgcttgatt ggggtttcac
    18301 tagccaccca gtcagccaaa ccacctggtt caaaatccgc agcggtcata gctgtcttga
    18361 ctgctcctgg agcgatacca aagacctgaa tcccagcttc agcatagtct agagccaact
    18421 gcttggtgaa gccagccaag gcatgcttgg atgaagtata ggcgtgacca cctccacctg
    18481 ctaggctaga agcaatggaa cacatattga tgatgattcc ctttttattt tccagcattt
    18541 gtgtcaaata ataccgagtc aactctactg gaataatgta gttgatttca aaaatctctt
    18601 gaatgtcctg cgccgtttgt tccaacagtg gtttgtaatc atccaaaact ccagcagtat
    18661 tacacaaaac atccacctga gggcaccagt caaaaatagg ttccaagtcc aaggtcaaat
    18721 ctctctgtaa aaagtgaaaa tcaccctcta agagtggctt ttcaccttgg tcaactccat
    18781 aaacttgata gcccttctct aaaaagaggc gagcttgagc caatccgatc cctgaactca
    18841 ctcctgtaat gagtacacgt ttagtcatgc acttctaccc aatccgttgc caaaacatca
    18901 caaactgtcg ggctccacat ggaaaaacct tctccttcgc cagaaacgtt gattaggaaa
    18961 taaggtgtca tttcaagtgc aagcccattt tgctcgatgg tatcaaagag ttggacatag
    19021 ttttccgcac ctccccaacc agttcgtaca tattttctct tagcctttaa cccaggcagg
    19081 atctcttcaa atgtcatgtt tttctccttt aattctacat tcttcattta attataacaa
    19141 aaaaaccgct ttaaaacggc tttttgactg tgatttattt acatcttctt ctacttacgg
    19201 caaattattc cctgcagcaa gataaatttc ataccattct tttcttgtta agctaaagtt
    19261 tgccgcttgg ctaacttctc tcaagtgctt aggatttgtg gtacctacaa ctgcctgcat
    19321 ttttgctgga taacgcaata tccaagaaat ggcaatagtt gaagaggtta ctccatattt
    19381 aatagctaaa cgatcaagta cttgatttaa agcttgaaat ttctcatttc caacaaaatt
    19441 ccctttaaaa tacccgaatt gcaagacaga ccatgcttga atgaccacat cgtgtaattg
    19501 gcaatattca aaaatgctgc catctcgcat agctgcttga ctatcttcca tattaacatg
    19561 aaaagctgat tcaaatcctg gagtaaaagc cgcactcaat tgtagctgat taacagctaa
    19621 cggctgcttg acatcttttt taagcaactc catcatcata ggattttgat tagaaactcc
    19681 aaaatctcga actttacctt gtttataaag gagattaaag gcttctgcta cttggtcaga
    19741 ttccatcaaa gcatctggtc gatgaaggag caagctatct agatgatcaa tcttcaatct
    19801 ttgcaaaata ccgtctactg attttataat atagtcctta gaaaaatcaa aataggtaaa
    19861 ttcttcaatg cgaatgccac atttggactg aatccacatc ttttctctta aatctggacg
    19921 attttttagg acaagaccta acagttcttc acaacgacca cgaccataaa tatcagccaa
    19981 gtcgaaggca ttgattccaa cagaaagtgc tgtttctaca agctcttcaa cttcttttac
    20041 agacttatct tttattctca tcattccgag aacaatttct gataattctt tgtcatcttg
    20101 accaagagtt atgtatctca tcaaattttt ctcctttaat ttctaacatt cttcccttca
    20161 ttataacaaa aaaccgcttt gcaacggctt tttgactata cttcactcca ttttatcttc
    20221 ttaaacccac ggaacaagac aaagattcca ataaagagga cagctaaagg aataactttt
    20281 gtaaggaaaa catttgaaat tcccatccac tcataataac ggagcagaga acccaccaca
    20341 agatgggcaa taatcatact gacaaatgga cgaaagaccg cttctttcca attccaaata
    20401 ccgataacta gcgaaatcgt aaagacagac aaactatccc agggagccgg aatataaaag
    20461 gctccttctt gtacaaaact tcctattcct acatatccca taacaactag cagaacaaga
    20521 atcccaacga caatataggt gccaattttc attttaggag aatcttggac taagcaccaa
    20581 cgtaagattg tggccacaag tccaaatcca atcagaaaaa taagaagttg ccctaaaaat
    20641 gtgagcaaat tgactgttaa gagaggacct ttagaaaaat cacttagtag ttgataataa
    20701 cgtaataccg ctaggataag aattggcgtc aaaagggact ctttgataga actgcgaggt
    20761 gctcccttga gaatctcttt cattattttt ttaggattct tacctagata atcctctgca
    20821 ctcatgccat ctcgttctgc ttctgagaaa tctagcatca tcaaatagat ctgctctctg
    20881 agatagtctt catcatagag aaatccagca agattaaaat tttcccacaa ctcctcaaaa
    20941 tacttttgat tctcctcaga aaactcatgt agcaaagcgc ttgtttcttc gtaatacttc
    21001 attttcttca tggtttaacc cccattctta atcccttcta ctttttgact caaatcgtcc
    21061 cattgttgcc aaaagactga gacacgctct tctccttctt tcattaatga aaaatacttc
    21121 cgatctggac catctagcga cgggcgcatg tcgcctctta tccattgatt tttttctaac
    21181 ttttgcaaca aaggataaat agttcctgga acgatagtat caaatccagc ctctcgcaaa
    21241 gtctgaacca actcataacc ataccgctct ttttgaccaa tcatatccaa gacacaacct
    21301 tcaagaacac cttttaatag ctgagtttct ttcatcactt ctcccttcta atctattttg
    21361 taatacctac tagtaacttc acctatagta tatcacttct acactagttt gtaaagcata
    21421 atagttaata ctcttcgaaa atctcttcaa accacgtcag cgtcgcccta ccgtatgtat
    21481 ggttactgac ttcgtcagtt tcatctacaa cctcaaaaca atgttttgag ctgacttcgt
    21541 cagtttcatc tacaacctca aaaac
//
LOCUS       DAVKDZ010000027        21476 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000027 DAVKDZ010000000
VERSION     DAVKDZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21476
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.028"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(<1..481)
                     /locus_tag="VWZ30_001050"
     CDS             complement(<1..481)
                     /locus_tag="VWZ30_001050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEU9334809.1"
                     /translation="MIKQIKAHLNKSIQSIIGQKVEFVKQDEQAFTRKRRLSLETMIR
                     TILGMGGKSLSKELLDARLTVSNSAFVQRRYQIKPEAFYALFKEFTAPIPLNTDFPIF
                     AADGSDICIPRNPMDTETSIQTQTDVKSYNLIHINALYDLTTGVYRDVSIQDKHAQHE
                     "
     gene            complement(590..1045)
                     /locus_tag="VWZ30_001051"
     CDS             complement(590..1045)
                     /locus_tag="VWZ30_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9334810.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWPGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDCRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(1065..2240)
                     /locus_tag="VWZ30_001052"
     CDS             complement(1065..2240)
                     /locus_tag="VWZ30_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9334811.1"
                     /translation="MKHFDTIVIGGGPAGMMSTISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTIIELEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVNGGEVLSLDVLPQLSEKNLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(2299..3144)
                     /locus_tag="VWZ30_001053"
     CDS             complement(2299..3144)
                     /locus_tag="VWZ30_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9334812.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRTVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            3269..3826
                     /locus_tag="VWZ30_001054"
     CDS             3269..3826
                     /locus_tag="VWZ30_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9334813.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            3845..4312
                     /locus_tag="VWZ30_001055"
     CDS             3845..4312
                     /locus_tag="VWZ30_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU9334814.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            4401..5030
                     /gene="upp"
                     /locus_tag="VWZ30_001056"
     CDS             4401..5030
                     /gene="upp"
                     /locus_tag="VWZ30_001056"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU9334815.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            5204..5794
                     /gene="clpP"
                     /locus_tag="VWZ30_001057"
     CDS             5204..5794
                     /gene="clpP"
                     /locus_tag="VWZ30_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEU9334816.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            5873..6121
                     /locus_tag="VWZ30_001058"
     CDS             5873..6121
                     /locus_tag="VWZ30_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEU9334817.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            6223..7383
                     /locus_tag="VWZ30_001059"
     CDS             6223..7383
                     /locus_tag="VWZ30_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9334818.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVIDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            7651..8520
                     /locus_tag="VWZ30_001060"
     CDS             7651..8520
                     /locus_tag="VWZ30_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9334819.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGIVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILGISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            8524..9480
                     /locus_tag="VWZ30_001061"
     CDS             8524..9480
                     /locus_tag="VWZ30_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9334820.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILTVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVTS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            9480..10244
                     /locus_tag="VWZ30_001062"
     CDS             9480..10244
                     /locus_tag="VWZ30_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9334821.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            10244..10954
                     /locus_tag="VWZ30_001063"
     CDS             10244..10954
                     /locus_tag="VWZ30_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9334822.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVASGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            11262..11918
                     /locus_tag="VWZ30_001064"
     CDS             11262..11918
                     /locus_tag="VWZ30_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9334823.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            12026..13121
                     /gene="prfB"
                     /locus_tag="VWZ30_001065"
     CDS             join(12026..12097,12099..13121)
                     /gene="prfB"
                     /locus_tag="VWZ30_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU9334824.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIVILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            13139..13831
                     /gene="ftsE"
                     /locus_tag="VWZ30_001066"
     CDS             13139..13831
                     /gene="ftsE"
                     /locus_tag="VWZ30_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU9334825.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            13824..14750
                     /locus_tag="VWZ30_001067"
     CDS             13824..14750
                     /locus_tag="VWZ30_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9334826.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEEAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            15036..17216
                     /locus_tag="VWZ30_001068"
     CDS             15036..17216
                     /locus_tag="VWZ30_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9334827.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPNQMTEV
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     NAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            17268..18083
                     /locus_tag="VWZ30_001069"
     CDS             17268..18083
                     /locus_tag="VWZ30_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9334828.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLSEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPL
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            18223..19566
                     /locus_tag="VWZ30_001070"
     CDS             18223..19566
                     /locus_tag="VWZ30_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9334829.1"
                     /translation="MSFTKFQFKNYIREALEELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            19781..20971
                     /locus_tag="VWZ30_001071"
     CDS             19781..20971
                     /locus_tag="VWZ30_001071"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU9334830.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
BASE COUNT         6473 a         3966 c         4637 g         6400 t
ORIGIN      
        1 gctcatgttg tgcatgtttg tcttgaatag agacatctct atacactcca gtcgtcaagt
       61 cgtatagggc atttatgtgt atgagattat aggatttaac atccgtttgg gtttggatag
      121 aggtttctgt atccatggga tttcgaggaa tacagatatc actcccatct gcagcgaata
      181 ttggaaaatc agtattaagt ggaataggtg ctgtaaattc tttaaatagg gcatagaaag
      241 cttcaggttt tatctgatag cgtctttgaa caaaggctga atttgaaact gtcagtctgg
      301 catctagtaa ttcttttgat agtgattttc ctcccatgcc cagaattgta cgaatcatag
      361 tttctagtga taaccttctt ttacgagtaa aggcctgttc atcttgtttg acgaactcaa
      421 ctttttggcc aatgatactc tgaatactct tattcaagtg ggcttttatc tgttttatca
      481 tgggaaatac ctccttaact atagtttatc acataaaaaa gagacccaaa tacagaattg
      541 tatttgaatt tcttaactta atgacattgg gaatttccta gcatattttt tacttcaaat
      601 cataaagcca gtcgtcaccg tttttgtagt aaaagaattc actgagatct tcttctagaa
      661 acacacgaag catatcagac atatcatcgg ttgcaagttt tagatgagaa agattttcaa
      721 agtcctccca ccaaactttc ccttcgtctg aagactggag ttcaccagta aagtgttctg
      781 tcttgtaaaa aaggacgaca taacgacaat ccttgtcgtc ataccagttt ttgataccac
      841 agagttgggg tttggaaatg atcagaccag tttcttcttt cacttcacga atgacagcat
      901 cgacaaagga ttcgccacgt tcaacatgac caccaggaaa agtaatgcca ggccagtcgg
      961 gactaactcg gtcttggacc aggaccttat ctccgttttt aatcatacac atgttaacaa
     1021 attcgactgc ctctcttctg ttcattcttc acaaccttta atctttaatc ataatgcaga
     1081 cttcccgcca cccagccggt acagagggca gaagtgatgt taaagccacc cgtgtgggca
     1141 ttgatatcca taacttcgcc tgcaaagtgg aggccaggta ccagcttact ttcaagggtt
     1201 ttaggattga tttccttgag actgactcca cccttggtaa caaaggactt tgcaagggac
     1261 atttttccag ttacaggaat tttaagttct ttaatggact ggacaagctg ttctcgttcc
     1321 ttttcagtca gctgtttgac tttttcagga tatccttgta caaaaaattc ggccaagcgt
     1381 tctggtaaca aggtttttaa agcgtttttc aaggattttt cccgattttc ttctagaaat
     1441 gtaaccaagt tcttctcaga aagttgaggc aaaacatcga gtgagagaac ctccccaccg
     1501 tttacaaagc tagacatgcg tagggcagca ggacctgaca aaccaaagtg ggtaaagagt
     1561 aaatcatgag tgatgacatg cttaccataa cttagggtca catcgtccag agaaatccct
     1621 tgtaaggctt tatgtggaaa atctgttaat aaaggacttt cagcagcctc aagctcgatg
     1681 atggtatgct taaaatggcg agcaatctcg tgaccaaaac cagtcgaacc agtcgaagga
     1741 taagacttac cacctgttgt gacaatgagt ttctcacaag tgaaggtttg atccgctgac
     1801 ttaaggacaa actggtcatc tactttttta acagaaacga tttctatttg agtagcaact
     1861 tgaccaccta gttcagtgat tttcttttcc aaagcttcga taatagtccg agacttgtca
     1921 ctggctggaa agacgcgtcc gtggtcttcg accttaagtt taacaccatt ttctgtaaaa
     1981 aagttgatga tgtcatgatt atcgaactgg gagaaaacac tgtaaagaaa gcgtccgttt
     2041 ccaggaattc cagctagcag gttgtctaag ctaccattgt tggtcacatt gcaacgtccc
     2101 ccaccagtcc cagctaattt ttttccaagt ttccgatttt tttcgatgag gagagttttc
     2161 tgtccataaa agctactgga aatcgtagac atcataccag caggtccccc accgatgaca
     2221 atagtatcaa aatgtttcat agctctattg taccacaaaa aaacaagaga tgatggtcac
     2281 ctcttgtcaa gaatgcaatt aatcaatttc atagcccatc agcaaaccgc cctcttctgc
     2341 atagaaactg cagagaccag aggttggtag aattttaata tccgcttgtg ggaaggtttc
     2401 acggattcgc tctgagagct gttgacaaca tttttcgtta ttgcgttggg ccatgacagt
     2461 acggccacca gcatatccag cttttactaa ctcatcatag gcagcttgaa ctgatttctt
     2521 tgatcccctt gctttttgta gcaattcgag agttccagtt tcactagctt ttccgaccat
     2581 acgaatgttg agaaggccaa cgaccgtacc gataagcttg ctcaaacggc cgttcttcac
     2641 caagttatcg actttggcta ggacaaagag caacttagtt ttttcttgat aggcggtgat
     2701 agcttcaacc acttcttcaa aagacaagcc ctggtcaatc aagtcattca atttttctac
     2761 gagtaggtca acttcaccac cagcagataa actatcaatc acatgaatct tagtgtcagg
     2821 atggtcttcc agataaatat tctttgctag ttgagcacta ttgtgactgc cagaaagggt
     2881 acctgtgatg gttactagga aaatgttttt ggcaccttca aatgctcgca aatagtcatc
     2941 tgggcttgga caagccgatt ttgaagcttc tgcagttgca tacatggttt ccatcatttg
     3001 gtcaatatcg agactggcgt catcaacaaa gacctgatca gctacttgaa tggttaaggg
     3061 gacacttaca aaggttgtgt taatagctgg tgttggcagt tgacgataat cacaaccaga
     3121 gtcagcaata atcttccaag tcatagaaat tctccatctt tgtcagttat acattgacaa
     3181 aggttctgtc tttttttaca attatatcat gaaaaccctt gaaacaaaag catcatccgt
     3241 ctgttgtgac aagtagaaag aaaatgttat gtctgaacgt agaatctctg aaaagtctct
     3301 tgaaaatctc agaaaatcaa accaagaatc caatttatta accagagaag ccattgaaac
     3361 agccctcttg caactcttgg aaaaaaagga actgaccaag attagtattt ctgaattggt
     3421 caaacgtgca ggtgtttcgc gtgcggcctt ttatcgcaat tatgactcca aagaggaaat
     3481 tttagagagc gtctttaaac gaactgtcca caatattatg gaacagctgc atcattacga
     3541 tttaaagaca gacctttatt tggtttgggt tcaccttttc cgggaggcca gaaaggaagc
     3601 tagagtaatt caattggcct tggattacca tctggaaaaa atctttgtcc aagccatgca
     3661 ggaatttcta gaaaaatacc atgggaaatc aaaaggtgtc agctcttatc ttcattcctt
     3721 ctggagctca gccatcgtct ctgtccttct aaaatggatc aaggatggca tgaaggtacc
     3781 agctgaaaag attgcagatt taggtttacc attttttaaa aaatagagaa aaaggagaaa
     3841 agagatgact gaaaaaagac tggcctggga tgagtatttt gcagcccaag ctctactaat
     3901 tgcgaatcgt tccacttgta aacgtgctaa agtgggcgcg attctggtaa aagataataa
     3961 ggttatttcc actggttaca atggttcggt gtcagggact gagcattgta ttgatcacga
     4021 atgtttggtc attgaaggcc actgcgttcg cacccttcac gctgaggtca atgctatcct
     4081 tcaaggtgct gagcgtggtg ttcccaaagg ctttacagcc tatgtaaccc attttccttg
     4141 tctcaactgt acaaaacaat tgcttcaggt cggctgcaag cgcgtggttt atatcaacca
     4201 gtaccgaatg gacgactacg cccaatacct ttatcaagaa aaagggacag aattgaccca
     4261 tttaccactt gagacagtac agacagctct taaagaggct gatctaatgt aaaaattatc
     4321 aaaaataaat ggtttagaaa gatttttaaa ccgttttttg gtataataag aagaataaat
     4381 tgaaagaagg aattccaaaa atgggaaaaa ttgaagttat taatcaccca ctgattcaac
     4441 acaaattgtc aatcttgcgt cgtacagata cttctacaaa agcttttcgt gagctagtag
     4501 atgagattgc aatgttgatg gggtatgaag tacttcgtga tcttccacta gaagatgtgg
     4561 aaatcgaaac accaattaca aaaacagttc aaaaacaatt ggcaggtaag aaattggcca
     4621 tcgtcccaat cttgcgtgca ggtatcggga tggttgatgg tctcttgagc ttggttccag
     4681 ctgctaaagt tggccacatc ggtatgtacc gtgatgaaga aacacttcaa ccagttgaat
     4741 acttggtgaa attgcctgag gacattgacc aacgtcaaat ttttgtagta gacccaatgt
     4801 tggcaacagg tggctcagca atcttggctg ttgattctct taaaaaacgt ggcgcatcaa
     4861 atatcaaatt tgtctgcctt gtatctgctc cagagggtgt aaaagccctt caagaagctc
     4921 atccagatgt agaaatcttt acagcagcct tggatgaacg tttgaacgaa cacggttata
     4981 tcgttccagg tcttggagat gctggagacc gcttgttcgg tacaaaataa gatcgaaaag
     5041 aagaatgact tggaaaagta tttctagtca cgaaaggagg ttgggttttt gtttctgtct
     5101 aatgaaagca gagcaaaaat ttgacctttt ttgaccaaga tattataata gtcttatctt
     5161 cagtcatttg accaacaaaa ttaaaactca aaaggagaaa tgaatgattc ctgtagttat
     5221 tgaacaaaca agccgtggag aacgttctta cgatatttac tcacgtcttc tcaaagaccg
     5281 tatcattatg ctgacaggtc cggttgaaga caatatggct aactctgtta ttgcccaatt
     5341 gcttttcttg gatgcccaag atagtacaaa agatatttac ctttatgtca atacaccagg
     5401 tggttctgtt tcagctggtt tggcaatcgt agataccatg aactttatca aggcagatgt
     5461 ccaaaccatt gttatgggaa tggctgcatc tatggggact gtcatcgcat caagtggagc
     5521 aaaaggcaaa cgtttcatgc ttccaaatgc tgaatacatg attcaccaac caatgggcgg
     5581 tacaggtggt ggtacccaac aaactgatat ggctatcgct gcagaacact tgctcaaaac
     5641 tcgtaatacc ttggaaaaaa tcttggctga aaattcaggt cagtcaatgg aaaaagtcca
     5701 tgcagatgca gaacgtgata actggatgag cgcccaggaa acacttgaat atggctttat
     5761 tgatgaaatt atggccaaca attcattgaa ctaatgatga tagaaggcaa actcgactgg
     5821 gtttgctttt tttggtataa tagggagaga tttcttagaa agaggattta tcatgtttga
     5881 aaaagtcaat cgctctggct tgattatcta tctttactat aatcgtgatg ccaaaaaact
     5941 gcaggattat ggagatatta cctatcattc caagaaacat cgttacttac aactctatgt
     6001 tccaactcaa gaagtggagc aattggtcgg acgcttgagc aaggaaaaat ttattaaaaa
     6061 agttagggtt tgtcatatcc aagagttgga aacacccttt gtgggcaatc tttatcgata
     6121 ggaaaacgtt atcatcgaaa aagttcaagg aaaacattga caattttctg ataattcggt
     6181 atattcttaa cagactattt aagaaataag gagacaaaaa agatgaagaa aaaatttgcc
     6241 ctatcgtttg tggcgcttgc aagtgtagca cttcttgcag cctgtggaga agtgaagtct
     6301 ggagcagtca acactgctgg taactcagta gaggaaaaga caattaaaat cgggtttaac
     6361 tttgaagaat caggttcttt agctgcatac ggaacagctg aacaaaaagg tgcccaattg
     6421 gctgttgatg aaatcaatgc cgcaggtggt atcgatggaa aacaaatcga agtaatcgat
     6481 aaagataata agtctgaaac agctgaggct gcttcagtta caactaacct tgtaacccaa
     6541 tctaaagtat cagcagtcgt aggacctgcg acatctggtg cgactgcagc tgcggtagcg
     6601 aacgctacaa aagcaggtgt tccattgatc tcaccaagtg cgactcaaga tggattgact
     6661 aaaggtcaag attacctctt tattggaact ttccaagata gcttccaagg aaaaattatc
     6721 tcaaactatg tttctgaaaa attaaatgct aagaaagttg ttctttacac tgacaatgcc
     6781 agtgactatg ctaaagggat tgccaaatct ttccgcgagt catacaaggg tgaaatcgtt
     6841 gcagatgaaa ctttcgtagc aggtgacaca gacttccaag cagcccttac aaaaatgaaa
     6901 gggaaagact ttgatgctat cgttgttcct ggttactata atgaggctgg taagattgta
     6961 aaccaagcgc gtggcatggg aattgacaaa ccaatcgttg gtggtgatgg attcaacggt
     7021 gaggagtttg tacaacaagc aactgctgaa aaagcatcaa acatctactt tatctcaggc
     7081 ttttcaacta ctgtagaagt ttcagctaaa gctaaagcct tccttgacgc ttaccgtgct
     7141 aagtacaatg aagagccttc aacatttgca gccttggctt atgattcagt tcaccttgta
     7201 gcaaacgcag caaaaggtgc taaaaattca ggtgaaatca aggataacct tgctaaaaca
     7261 aaagattttg aaggtgtaac tggtcaaaca agcttcgatg cagaccacaa cacagtcaaa
     7321 actgcttaca tgatgaccat gaacaatggt aaagttgaag cagcagaagt tgtaaaacca
     7381 taatagaaaa atgttgaaat agggaatgag cctttgactc actccctgtt tcgatgttta
     7441 atactcttcg aaaatctctt caaactgtgt caacgtcgcc ttggattata tatgtgactg
     7501 acttcgtcag tcttatctac aacctcaaag cagtgctttg agcaacctgc ggctagtttc
     7561 ctagtttgct ctttgatttt cattgagtat aagaacctat caaaaagtga gggaaacccc
     7621 tcggaattat aaatagaaag agtgaatctt atgctccaac aactcgtaaa tggtttgatt
     7681 ctaggtagtg tttacgcgct gttagcccta ggatatacca tggtttacgg aattatcaag
     7741 ctcatcaact tcgcccatgg tgatatttat atgatgggag cctttatcgg ttatttcttg
     7801 atcaattctt tccaaatgaa tttctttgta gcgcttattg tagctatgct agcgacagct
     7861 attcttggta tcgtgattga gtttcttgct tacagacctt tgcgccactc tactcgtatt
     7921 gctgttttga ttacggctat tggggtttct ttcctattgg agtatggaat ggtctatctg
     7981 gttggtgcca atacccgtgc cttccctcaa gcgattcaaa cagttcgata tgatttggga
     8041 ccaattagct taacaaatgt gcagttaatg attttgggca tttccttgat tttgatgatt
     8101 ttgttacaag tcattgtcca aaagactaag atggggaaag ccatgcgtgc agtatcagta
     8161 gatagcgacg cggcgcaatt gatggggatc aatgtaaacc gtacgattag ctttaccttc
     8221 gctttgggtt ctgctcttgc gggtgcggct ggtgttctga ttgctcttta ttataactct
     8281 cttgagcctt tgatgggggt tactccaggt cttaaatctt tcgttgccgc agtacttggt
     8341 ggtatcggaa ttattcctgg tgctgctctt ggtggctttg tgattggtct attggaaacc
     8401 tttgcgacag cctttgggat gtcagatttc cgtgatgcca ttgtttatgg aatcttgttg
     8461 ttgatcttga ttgtccgccc agctggtatc cttggtaaga atgtgaaaga gaaggtgtaa
     8521 acgatgaagg aaaatttaaa agttaatatt ctatggttac tccttttgtt agctggctat
     8581 agcttgatta gtgtactggt ttcagtcgga gtacttaatc tattctatgt acagatttta
     8641 caacaaattg gaattaatat tattttgact gttggtctca acttaatcgt tggtttttca
     8701 ggacaatttt cacttggtca tgctggtttc atggcgattg gtgcctatgc agcagctatt
     8761 attggttcta aatcaccaac ctacggtgcc ttctttggag ctatgcttgt aggggctttg
     8821 ctttcaggag cagttgcctt acttgtcggc attccaacct tgcgcttgaa gggggactat
     8881 cttgcggtag caactctggg tgtttctgaa attatccgta tctttatcat caatggtgga
     8941 agccttacaa atggtgcggc aggtatctta gggattccta actttacaac ttggcaaatg
     9001 gtttacttct ttgtcgtgat tacaaccatt gcaaccttga acttcttgcg tagcccaatt
     9061 ggtcgttcaa ccctctctgt tcgtgaagat gaaatcgctg ctgagtcagt tggggttaat
     9121 acgactaaaa ttaaaatcat cgcttttgtc tttggtgcca ttactgcaag tattgctggg
     9181 tcacttcagg caggatttat cgggtctgtt gtaccgaaag attacacctt catcaactca
     9241 atcaacgttt tgattattgt tgtatttggt ggactcggtt ccattacagg tgcgattgtt
     9301 tcggctattg ttctgggaat tttgaatatg cttctccaag atgttactag tgtgcgtatg
     9361 attatttacg ctttggcctt ggtattggta atgattttca gaccaggtgg actccttgga
     9421 acatgggaac tgagcctatc acgtttcttt aaaaaatcta agaaggagga acaaaactaa
     9481 tggcattact tgaagtaaaa cagttaacca aacattttgg tggtctaaca gctgttggag
     9541 atgtgactct tgaattgaac gaaggggaac tggttggatt aatcggtcca aacggagctg
     9601 ggaaaaccac ccttttcaac cttttgaccg gtgtttatga accaagcgag ggaacagtaa
     9661 ccctagatgg tcaccttttg aatgggaaat caccttataa gattgcctct ttgggacttg
     9721 gacgtacttt ccaaaatatc cgtctcttta aagatttaac agttttagat aatgttttga
     9781 ttgcttttgg aaaccatcac aaacagcatg tttttactag tttcttacgc ttaccagctt
     9841 tttacaagag tgaaaaagaa ttaaaggcta aagctttgga attgttgaaa atctttgatt
     9901 tagatggtga tgcagagact cttgctaaaa atctttccta cggacaacaa cgtcgtttgg
     9961 aaattgttcg tgcccttgct acggaaccta aaattctctt cttagatgaa ccagcagcag
    10021 gtatgaaccc acaggaaaca gccgaattga ctgagttaat tcgtcgtatc aaagatgaat
    10081 ttaagattac aatcatgttg attgaacacg atatgaatct ggtcatggaa gtaacagaac
    10141 gtatctacgt acttgaatat ggccgtttaa ttgctcaagg aactccagac gaaattaaga
    10201 ccaataaacg cgttatcgaa gcttatctag gaggtgaagc ctaatgtcta tgttaaaagt
    10261 tgaaaatctt tctgtgcatt acggtatgat ccaagcagtc cgtgatgtaa gctttgaagt
    10321 taatgaagga gaagttgttt cccttatcgg tgccaacggt gcaggtaaga caactattct
    10381 tcgcaccttg tcaggtttgg ttcgaccaag ttcaggaaag attgaatttt taggtcaaga
    10441 aatccaaaaa atgccagctc agaaaatcgt ggcaagtggt ctttcacaag ttccagaagg
    10501 acgccacgtc tttcctggct tgactgttat ggaaaatctt gaaatgggag ctttcttaaa
    10561 gaaaaatcgt gaagaaaatc aagctaactt gaagaaggtt ttctcacgct ttcctcgtct
    10621 tgaagaacgg aagaaccaag atgcagccac tctttcaggg ggggaacaac aaatgcttgc
    10681 catgggacgc gccctcatgt caacaccaaa acttcttctt ttagatgaac catcaatggg
    10741 acttgcccca atctttatcc aagaaatttt tgatatcatt caagatattc agaagcaagg
    10801 aacaacggtc ctcttgattg aacaaaatgc caataaagca cttgcaatct ctgaccgagg
    10861 atatgtactg gaaacaggga aaatcgtcct atcaggaaca ggaaaagaac tcgcttcatc
    10921 agaagaagtc agaaaagcat atctaggtgg ctaaaacaat ccagtggatt gttttagtcg
    10981 gcagatggag attacgaagt aatcatcaat atagtccggg ggaccttttt agtcggtaga
    11041 ttgagattgc aaacaaatct gcatctacat tgaaagctta atttctaata attgaaaaaa
    11101 tcgaatgaaa aatttcttac cttcattcac agagctcgat ttcagagctc tttttgctag
    11161 cttattcata cttttctgaa tttcgaaaaa gaaatgtaag cgtttgatag atttacaaaa
    11221 agattgtata atagggataa gaatagaaaa ggagaagtct catggcagtt aaagatttta
    11281 tgacccgcaa ggtagtttat attagtccag atacaacagt atctcatgca gcagatttga
    11341 tgagagagca aggtttgcac cgtctgcctg ttatcgaaaa tgatcaatta gttggtttgg
    11401 tgactgaggg aaccattgca caagcaagtc catctaaagc aacaagtctt tctatctatg
    11461 agatgaatta tcttctgaat aagacaaaag taaaagatgt catgattcgc gatgttgtca
    11521 ctgtctcagg ctatgctagt ctagaagatg caacttatct gatgttgaaa aataagatta
    11581 gtattctccc tgtcgtagat aaccatcaag tatacggagt tattactgac cgtgacgttt
    11641 tccaagcctt tcttgaaatt gcaggttatg gcgaagaagg gattcgtgta cgctttgtta
    11701 cagaagatga agttggtgtt cttggaaaaa ttgtttcttt gattgtagaa gaaaatttga
    11761 atatctccca tacagtcaat attccgcgta aggatggtaa ggtgattatc gaagtgcaaa
    11821 tcgatggatc aattgattta ccagccttga aagaaaaatt tgaagcaaat ggtattcaag
    11881 tggaagaaat cgctcgcact tcagcaaaag tcttgtaaga agggaagccc aaaggcttct
    11941 tttttcatga aaaggggatt agagaaaaag atggaaagaa atgataaaat atgctataat
    12001 gaaataatgt aaaaaaggag tatttatgga catttcagta attcgtcaga aaattgacgc
    12061 aaatcgtgaa aaattagctt ctttcagggg gtctctttga cctcgaaggg ctagaggaag
    12121 agattgtcat cttggaaaac aagatgacag aacctgattt ttggaacgat aatattgcgg
    12181 cccaaaaaac gtcgcaagaa ttaaatgaat taaaaaacac ttacaatacc ttccataaga
    12241 tggaagagtt gcaggatgaa gtcgaaattt tattggattt tttggctgaa gacgagtcag
    12301 tgcatgatga actggtagcg cagttagccg aacttgataa gataatgacc agctacgaga
    12361 tgactctact cttgtcagaa ccttatgacc acaacaatgc catcttggaa atccatccag
    12421 gttctggtgg tactgaggcg caggactggg gtgatatgtt gcttcgtatg tatactcgtt
    12481 atggtaatgc taaaggcttt aaagtggaag tgttggatta ccaagcaggt gatgaggctg
    12541 gtattaagtc ggtaacttta tcatttgaag ggcctaatgc ctatggtctc ctcaagtcag
    12601 aaatgggtgt tcaccgctta gtgcgaatct caccatttga ctctgccaaa cgtcgccata
    12661 cctctttcac atctgtagaa gtgatgccag aattggatga tactattgaa gtggaaatcc
    12721 gtgaagatga tatcaagatg gataccttcc gttcaggtgg tgccggtgga caaaacgtca
    12781 ataaggtttc aacaggtgta cgtttaaccc acattccaac tggaattgtt gtccaatcaa
    12841 cagtggatcg tacccagtat ggaaatagag atcgtgccat gaagatgttg caggctaagc
    12901 tctatcaaat ggagcaagag aagaaggctg cggaggtaga ttctctcaaa ggtgagaaaa
    12961 aggagatcac ttggggaagc caaatccgtt cttatgtctt cacgccttat actatggtaa
    13021 aagatcaccg aactagcttt gaggttgctc aggtagataa ggttatggat ggggacctag
    13081 atggttttat cgatgcttat ctcaagtggc gaattagcta agatagaaag gaactcacat
    13141 gtcaattatt gaaatgagag atgtcgttaa aaaatacgac aacggaacga ctgctctacg
    13201 cggtgtttcg gtcagcgttc aaccggggga atttgcttac atcgtaggac cttcaggagc
    13261 agggaagtca acttttattc gttctctgta tcgtgaagta aaaatcgata aaggaagcct
    13321 atcagttgct ggttttaatc tggttaagat caaaaagaaa gatgtcccgc ttctacgtcg
    13381 tagtgttggg gttgtcttcc aggattataa attgttacca aagaaaactg tctatgaaaa
    13441 tattgcttac gctatggaag taatcgggga aaatcgccgt aatatcaaaa gacgagtgat
    13501 ggaagttttg gacttggttg gattgaagca taaggttcgt tctttcccaa atgaactctc
    13561 aggtggggag caacagcgga ttgcgattgc gcgtgcaatt gtaaataatc ccaaagtatt
    13621 gatagctgat gagccaacag gaaatctgga tccggataat tcatgggaaa ttatgaatct
    13681 cttggaacgg attaacctac aaggaacaac tattttgatg gcgactcata atagccagat
    13741 tgtaaatacc ttgcgccacc gtgtcattgc cattgaaaat ggccgtgtcg ttcgtgacga
    13801 atcaaaagga gagtatggat acgatgatta gtagattttt tcgccattta tttgaagcct
    13861 taaaaagttt gaaacgaaat ggttggatga cagtagctgc tgtcagttca gtcatgatta
    13921 ctttgacctt ggtggcaata tttgcatctg ttattttcaa tacagcgaaa ctagctacag
    13981 atattgaaaa taatgtccgt gtagtagttt atatccgaaa ggatgtggaa gataatagtc
    14041 agacaattga aaaagaaggt caaactgtta caaataatga ctaccacaag gtatatgatt
    14101 ctttgaagaa catgtctacg gttaaaagtg ttaccttttc aagtaaagaa gaacaatatg
    14161 aaaaattaac cgagataatg ggagataact ggaaaatctt tgaaggagat gctaatcctc
    14221 tctatgatgc ctatattgta gaggcaaaca ctccaaatga tgtaaaaact atagccgaag
    14281 aggctaaaaa aattgaaggt gtctctgagg ttcaagatgg cggtgccaat acagaaagac
    14341 tcttcaagtt agcttcattt atccgtgttt ggggactagg gattgctgct ttgttaattt
    14401 ttatcgcagt tttcttgatt tcaaatacca ttcgtattac cattatttcc cgcagtcgcg
    14461 aaattcaaat catgcgcttg gtcggagcta aaaacagtta tatccgtgga ccgttcttgt
    14521 tagaaggagc ctttatcggt ttattgggag ctatcgcacc atctgttttg gtctttattg
    14581 tttatcaaat tgtttaccaa tctgtcaaca aatcgttggt agggcaaaat ctatccatga
    14641 ttagtccaga tttatttagt ccgttgatga ttgccctact atttgtgatt ggggttttca
    14701 ttggttcatt gggatcagga atatccatgc gccgattctt gaagatttag gtaaaatagc
    14761 tgcttttatg aggagattgc aaaatctcct tttttgctac aagagttttt gaaaagagat
    14821 gcgtagaaga aaagggcttc caaagaagtc ccccagagaa gacttcttga tggctaattt
    14881 gaacttgaaa tttggcgcac tagaaatttt ttagaaaaaa gtgtaatttt tttataaaag
    14941 cctttacaaa aaaaattaaa gtggtataat taaatcatag aaggtgcaaa cgttttcgtg
    15001 aaacgtttgc ctaaataaaa ataaaggaga tttgaatgat gaaagataca ttcaaaaatg
    15061 tcttgtcttt cgaattttgg caaaaattcg gtaaggcttt gatggtagtt atcgcggtta
    15121 tgccggctgc tggtttgatg atttcaatcg gtaagtctat cgtgatgatt aacccaacct
    15181 ttacaccact tgtcatcaca ggtggaattc ttgagcaaat cggttggggg gttatcggta
    15241 accttcacat tttgtttgcc ctagccattg gaggaagctg ggctaaagaa cgtgctggtg
    15301 gtgctttcgc cgctggtctt gccttcatct tgattaaccg tatcactggt acaatctttg
    15361 gtgtatcagg cgatatgttg aaaaatccag atgctatggt aactactttc tttggtggtt
    15421 caatcaaagt tgctgattac tttatcagtg ttcttgaagc tccagccttg aacatggggg
    15481 tattcgtagg gattatctca ggttttgtag gggcaactgc ttacaacaaa tactacaact
    15541 tccgtaaact tcctgatgca ctttcattct tcaacgggaa acgtttcgta ccatttgtag
    15601 ttattcttcg ttcagcaatc gctgcaattc tacttgctgc tttctggcca gtagttcaaa
    15661 caggtatcaa taatttcggt atctggattg ccaactcaca agaaactgct ccaattcttg
    15721 caccattctt gtatggtact ttggaacgtt tgctcttgcc atttggtctt caccacatgt
    15781 tgactatccc aatgaactac acagctcttg gtggtactta tgacatttta actggtgcag
    15841 ctaaaggtac tcaagtattc ggtcaagacc cactatggct tgcatgggta acagaccttg
    15901 taaaccttaa aggtactgat gctagtcaat accaacactt gttagataca gtacatccag
    15961 ctcgtttcaa agttggacaa atgatcggtt cattcggtat cttgatgggt gtgattgttg
    16021 ctatctaccg taatgttgat gctgacaaga aacataaata caaaggtatg atgattgcaa
    16081 cagctcttgc aacattcttg acaggggtta ctgaaccaat cgaatacatg ttcatgttca
    16141 tcgcaacacc tatgtatctt gtttactcac ttgttcaagg tgctgccttc gctatggctg
    16201 acgtcgtaaa cctacgtatg cactcattcg gttcaatcga gttcttgact cgtacaccta
    16261 ttgcaatcag tgctggtatt ggtatggata tcgttaactt cgtttgggta actgttctct
    16321 ttgctgtaat catgtacttt atcgcaaact tcatgattca aaaattcaac tacgcaactc
    16381 cagggcgcaa cggaaactac gaaactgctg aaggttcaga agaaaccagc agcgaagtga
    16441 aagttgcagc aggctctcaa gctgtaaaca ttatcaacct tcttggtgga cgtgtaaaca
    16501 tcgttgatgt tgatgcatgt atgactcgtc ttcgtgtaac tgttaaagat gcagataaag
    16561 taggaaatgc agagcaatgg aaagcagaag gagctatggg tcttgtgatg aaaggacaag
    16621 gggttcaagc tatctacggt ccaaaagctg acattttgaa atctgatatc caagatatcc
    16681 ttgattcagg tgaaatcatt cctgaaactc ttccaaacca aatgactgaa gtacaacaaa
    16741 acactgttca cttcaaagat cttactgagg aagtttactc agtagcagac ggtcaagttg
    16801 ttgctttgga acaagtaaag gatccagtat ttgctcaaaa aatgatgggt gatggatttg
    16861 cagtagaacc tgcaaatgga aacattgtat ctccagtttc aggtactgta tcaagcatct
    16921 tcccaacaaa acatgctttt ggtattgtga cggaagcagg tcttgaagta ttggttcaca
    16981 ttggtttgga cacagtaagt cttgaaggta aaccatttac agttcatgtt gctgaaggac
    17041 aaaaagttgc agcaggagat ctccttgtca cagctgactt gaatgctatc cgtgcagcag
    17101 gacgtgaaac ttcaacagta gttgtcttca caaatggtga tgcaattaaa tcagttaaat
    17161 tagaaaaaac aggttctctt gcagctaaaa cagcagttgc taaagtagaa ttgtaatata
    17221 cttgaggttg gaagctgtat tccaacctct tattttggga gaaaagaatg aaatttttaa
    17281 cactcaatac tcatagttgg atggagaaag aagcagagga aaaattccag attttgcttg
    17341 aagatattct tgaaaaggac tatgatttga tttgtttcca agaaattaat caggagatga
    17401 cctcgtcaga ggtggaggtt aatgaccttt atcaagcttt gccagcagct gagcctattc
    17461 accaagatca ctatgttaga ctcttggttg aaaagttatc tgagcaaggg aaaaattact
    17521 actggacctg ggcctataac catatcggct atgaccgcta ccacgaaggt gtggctatct
    17581 tgtctaaaac acctattgaa gccagagaaa ttttggtttc agatgtggat gatccaacag
    17641 actatcatac tcgccgtgtt gccctagctg aaactgtagt cgatggcaag gagctagcag
    17701 ttgccagtgt tcatctctct tggtgggata aaggttttca agaagaatgg gcacgatttg
    17761 aggctgtctt gaaaaaattg aacaagccac ttttactagc tggagatttt aacaacccag
    17821 caggtcagga aggttaccaa gctattttag ctagtccatt aggcttacaa gacgcatttg
    17881 aagttgctca agagaaaagt ggtagctata ctgttccgct tgaaattgat ggctggaaag
    17941 ggaacactga accccttcga atcgattatg tctttactac caaagagtta gcggtggaaa
    18001 atttacatgt cgtatttgat ggtaacaaga gtccacaagt gagtgatcac tatggcttga
    18061 atgctatgtt aaactggaaa taataactga aaagaggttg gaactataaa attccagcct
    18121 tttcttacta gagaagctac tggaaatagc ctaaataagt gagactactg taatggaata
    18181 aaatatggta taattgataa gatagataga atcgaggatg ttatgtcatt tacgaaattt
    18241 caatttaaaa actatattag agaagccttg gaggagttaa aatttacaac tccaacagag
    18301 gtgcaagaca agttgattcc tattgttttg gcaggtcgtg acctagtagg agaatcaaaa
    18361 acaggttcag gtaagactca tactttcttg ttaccgattt tccagcaatt agatgaagct
    18421 agcgatagtg tacaagcagt gattactgca ccgagtcgtg agttggctac tcaaatttac
    18481 caagtagcgc gtcagatttc agctcactca gatgtcgaag ttcgtgtggt taattatgtg
    18541 ggtggtacgg ataaggctcg ccagattgag aaattggcaa gcaatcagcc tcatattgtt
    18601 attggaacac caggccgtat ctacgacttg gttaaatctg gtgatttagc tattcataaa
    18661 gccaagacat ttgttgttga tgaagcggat atgaccttgg atatgggatt cttggaaact
    18721 gttgataaga ttgctggcag tcttccaaaa gacttgcaat tcatggtctt ctcagcgact
    18781 atcccacaaa aactgcaacc attcttgaaa aaatacttat ccaatcctgt tatggagaaa
    18841 attaagacca aaacggttat ttctgacacc attgataatt ggttgatttc gaccaaggga
    18901 catgataaga atgctcaaat ttaccagttg actcagttga tgcagccgta tttggcaatg
    18961 atttttgtta acactaaaac gcgtgctgat gaattgcatt catatctgac tgctcaaggc
    19021 ttgaaggttg caaaaatcca tggcgatatt gcccctcgtg aacgcaagcg aatcatgaat
    19081 caggtgcaaa atctggattt tgagtatatt gtcgcaacag atttggcagc acgtgggatt
    19141 gacattgaag gtgtcagcca tgtcatcaat gatgccattc cgcaagactt atcttttttt
    19201 gttcaccgtg ttggtcgtac tggacgaaat ggcctaccag gtacagctat taccctttat
    19261 cagccaagtg atgactcgga tatccgtgag ttggagaaat tgggaatcaa gtttagtcct
    19321 aagatggtca aagacgggga atttcaagat acctatgacc gtgatcgtcg tgccaaccgt
    19381 gagaaaaaac aagataaact tgatatcgaa atgattggtt tggttaaaaa gaaaaagaaa
    19441 aaagtcaaac cgggttataa gaagaaaatt caatgggcgg ttgatgaaaa gcgccgtaaa
    19501 accaagcgtg ctgaaaatcg cgctcgcggt cgtgcagagc gtaaagctaa acgtcaaaca
    19561 ttttaataga aattgttgga gtactgagct ccaacttttt gctttatgag aacgaactat
    19621 ctaaaccgaa acactacatt aaagactgca aattgcgatt aaaaatgcta taatgataaa
    19681 gttatatagt cccgataaga tggtaggtat ttattacgaa gagttttcct atcagtactt
    19741 tgtaactcta taacaatatt ttttaagggg ggacattttt atgtcagaac gtaaattatt
    19801 cacgtctgaa tctgtatctg aggggcatcc ggataagatt gcagaccaaa tttcagatgc
    19861 gattttggat gctattttag caaaggatcc agaggcgcac gttgctgctg aaacagctgt
    19921 atatactggt tctgtccacg tttttggtga aatttctaca aatgcctatg tggatattaa
    19981 ccgtgtggtt cgtgatacca ttgcagagat tggttatacc aatacagaat atggattttc
    20041 tgctgagacg gtgggagtac acccatcttt ggtggaacaa tctcctgaca tcgctcaagg
    20101 tgttaacgaa gccttggagg ttcgtggaaa tgctgatcaa gatccactgg acttgattgg
    20161 agcaggtgac caagggctca tgtttggatt tgcagtagat gaaacagaag agcttatgcc
    20221 attgccaatt gcactcagtc ataaattggt tcgtcgtctg gcagaacttc gtaagtctgg
    20281 agaaattagc tatctccgtc cagatgcaaa atcacaagtt acagttgagt acgatgaaaa
    20341 tgaccgtccg gtacgtgtag atacagtcgt tatttctact cagcatgatc cagaggccac
    20401 taatgaacaa atccatcaag atgtgattga caaggtcatc aaagaagtta ttccatcttc
    20461 ttatcttgat gataagacaa aattctttat caatccgaca ggtcgttttg taatcggtgg
    20521 tcctcaaggg gactcaggtt tgactggtcg taagattatt gtagatactt atggtggcta
    20581 ctctcgtcat ggtggtggtg ccttctctgg taaagatgcg actaaggtgg atcgttcagc
    20641 ctcttatgcg gctcgctata ttgccaagaa tatcgttgca gcaggccttg ctaagaaggc
    20701 agaagtgcag ttggcctatg ctatcggtgt tgcgcaacct gtttctgttc gtatcgatac
    20761 tttcggtaca ggaacagtag ctgaaagtca acttgaaaaa gcggctcgtc aaatctttga
    20821 ccttcgccct gcagggatta tccaaatgct ggacctcaag cgtccaattt accgtcaaac
    20881 atcggcttac ggtcacatgg gacgtacaga tattgatctt ccatgggaac gtttggataa
    20941 ggtagatgct ttgaaagaag cagtaaaata agattttaag aggggaacgt cctctctttt
    21001 ttatagtttt taactatact gggatactgt tctgaaaatc cattttgcga aagtagagat
    21061 ttacatgtat agtagattga aactagaata gtacacctca acttctaaaa cattgttagc
    21121 aatcaatttg actgtcctga tcgatttctc ctgttcttgt ttcattttac tatatttctt
    21181 taaaaatgat aaaggttaag atttctcctc gtaatagata atcttgggga tatttcaatc
    21241 caaagtttta ttcgttatca cttgactatt gcaaggtttt ctagagcaac agagtcatgg
    21301 aatggactca tggtggagat ttctccttgt tgcttggact tcattcaaaa gtctgttacc
    21361 caagccttgt tcaaacttct aatacactag ctgtttccat agcatgactt ctgtactaga
    21421 ctttcttttc cgaataaata gatagaacca cagaatctag taaacctaga attaaa
//
LOCUS       DAVKDZ010000028        21001 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000028 DAVKDZ010000000
VERSION     DAVKDZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21001
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.029"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            243..900
                     /locus_tag="VWZ30_001072"
                     /pseudo
     CDS             243..900
                     /locus_tag="VWZ30_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            890..1213
                     /locus_tag="VWZ30_001073"
     CDS             890..1213
                     /locus_tag="VWZ30_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU9334831.1"
                     /translation="MVSKYLLLAVIFSGLVTWIPRMIPCILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLAF"
     gene            1317..2147
                     /locus_tag="VWZ30_001074"
     CDS             1317..2147
                     /locus_tag="VWZ30_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9334832.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPKPFIYEENGELTGYEIEVVRAIFKDSDKYNVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2301..3155
                     /locus_tag="VWZ30_001075"
     CDS             2301..3155
                     /locus_tag="VWZ30_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9334833.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKSLFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            3255..4628
                     /locus_tag="VWZ30_001076"
     CDS             3255..4628
                     /locus_tag="VWZ30_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU9334834.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQRHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEDRMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            4621..5682
                     /locus_tag="VWZ30_001077"
     CDS             4621..5682
                     /locus_tag="VWZ30_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU9334835.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            5684..6376
                     /locus_tag="VWZ30_001078"
     CDS             5684..6376
                     /locus_tag="VWZ30_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9334836.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6406..6975)
                     /locus_tag="VWZ30_001079"
     CDS             complement(6406..6975)
                     /locus_tag="VWZ30_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9334837.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            7110..7724
                     /locus_tag="VWZ30_001080"
     CDS             7110..7724
                     /locus_tag="VWZ30_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9334838.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSFYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATFLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLETVYEIIHEKLATK"
     gene            7774..9358
                     /locus_tag="VWZ30_001081"
                     /pseudo
     CDS             7774..9358
                     /locus_tag="VWZ30_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843414.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            9370..10656
                     /locus_tag="VWZ30_001082"
     CDS             9370..10656
                     /locus_tag="VWZ30_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9334839.1"
                     /translation="MYKVLIVDDEYMVTEGLKRLIPFDKWDMEVVATVSHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVGY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDGAGFVSYLGDKENWWIGLSKEK
                     QGSFTTPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKALFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFGHLKADDLTDIVKTIHAIQSFDELVPYIKGTLTSFFGQYRMNENVVSVLEVIGRD
                     YQKELSIKDISKDLFINPVYLGQSIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKLPKAYRKQVETIL"
     gene            10717..11250
                     /locus_tag="VWZ30_001083"
     CDS             10717..11250
                     /locus_tag="VWZ30_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9334840.1"
                     /translation="MKKKPIYLWVLLILSALISVSSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            11328..11798
                     /gene="nrdI"
                     /locus_tag="VWZ30_001084"
     CDS             11328..11798
                     /gene="nrdI"
                     /locus_tag="VWZ30_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEU9334841.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKYVAAIIADLYELEKEN"
     gene            complement(12091..13353)
                     /locus_tag="VWZ30_001085"
     CDS             complement(12091..13353)
                     /locus_tag="VWZ30_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU9334842.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(13663..14121)
                     /locus_tag="VWZ30_001086"
     CDS             complement(13663..14121)
                     /locus_tag="VWZ30_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEU9334843.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFLLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREDKKVDHINQLLEHRK"
     gene            complement(14118..14558)
                     /locus_tag="VWZ30_001087"
     CDS             complement(14118..14558)
                     /locus_tag="VWZ30_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9334844.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            14914..16863
                     /gene="mutL"
                     /locus_tag="VWZ30_001088"
     CDS             14914..16863
                     /gene="mutL"
                     /locus_tag="VWZ30_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU9334845.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(17188..17655)
                     /locus_tag="VWZ30_001089"
     CDS             complement(17188..17655)
                     /locus_tag="VWZ30_001089"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU9334846.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWIPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(17656..18861)
                     /locus_tag="VWZ30_001090"
     CDS             complement(17656..18861)
                     /locus_tag="VWZ30_001090"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU9334847.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMIENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIEKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQILKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANAYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(18881..19516)
                     /gene="ribE"
                     /locus_tag="VWZ30_001091"
     CDS             complement(18881..19516)
                     /gene="ribE"
                     /locus_tag="VWZ30_001091"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU9334848.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTWEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(19501..20601)
                     /gene="ribD"
                     /locus_tag="VWZ30_001092"
     CDS             complement(19501..20601)
                     /gene="ribD"
                     /locus_tag="VWZ30_001092"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU9334849.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCKILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPIGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(20734..20848)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         6075 a         4007 c         4529 g         6390 t
ORIGIN      
        1 aatagtccat gctgtggctg gattcttttc agcctactta tactcttcga aactctcttc
       61 aaaccacgtc agcttcacct tgccgtaggt atggttactg acttcgtcag ttctatccac
      121 aacctcaaaa cagtgttttg agctgactac gtcagttcta tctgcttttc attgagtatt
      181 attaagacaa tatgaggaga aagatgaaag aaaaaggatt ttaggagggg gcgcaggcag
      241 ccatgccaac ggctcttggc tatgtcagta tcggcctggc ctgtggaatt atcggtgcgc
      301 cctatgtgac acctgttgag atgggcttga tgagtctctt tgtttatgct gggagtgccc
      361 agtttgccat gttggcactg attgcggttc aagctcctgt ggcagctatt gctatgacgg
      421 tttttctaat caatttgcgt ctctttttgt tgagtttaca cgcatcgact tatttccgtc
      481 ataccagtct ttggtacaat atcggtatgt ctagtatctt gacagatgag acctatggcg
      541 ttttgatggg tgaattggcc catacagaca aggtaaatcc gatgtggatg cacggaaaca
      601 atcttaacag ctatgtggct tggtttgtgg ggacagtagt cggaacggct ctgggtggcc
      661 tgctaccaaa tccagaaatc tttggcttgg attttgcccc tggttgggat gtttattggt
      721 atttttgctt cgcaattcca gatgatgcaa agacggattc ctgtccgcaa tctgctcatt
      781 atcctagcag ttgttgcggt gtccttcttt ttgctcttga cagtggtgtc tcagtcacta
      841 gctgttctgt ttgcgacgct acttggttgt agcatggggg tggttttaga tggtcagtaa
      901 gtatctttta ttagcagtta ttttctctgg cttggtgact tggattcccc gtatgattcc
      961 ctgcatctta gtcaagtata agggcttgcc tgcaatcgtt gagcgttttt tgaagttctt
     1021 gcccgtttcc attatctttg ccttgattct ttcaagcgta gtgacaggta aggttgggag
     1081 ccttcctcaa attaaatggc tagacttctt agccgtcttt ccaacagctt gggtagcctt
     1141 tcgctaccgc aatctagtcg gaacagttct ctttggagta gtcttgattg ccatcttgcg
     1201 tttggccttt taaatcaact ataaaaaaaa cttatcacag agatagatat catataatgg
     1261 cgtaaatgct ctttttctgt taagattata aggtattcta ttttggagga aatgacatga
     1321 aaaaaatcgt taaatactca tctcttgcag cccttgctct tgttgctgca ggtgtgcttg
     1381 cggcttgctc aggtggtgct aagaaagaag gagaagcagc tagcaagaaa gaaatcatcg
     1441 ttgcaaccaa tggatcacca aagccattta tctatgaaga aaatggcgaa ttgactggtt
     1501 atgagattga agtcgttcgc gctatcttta aagattctga caaatataat gtcaagtttg
     1561 aaaagacaga atggtcaggt gtctttgctg gtcttgatgc tgaccgttac aatatggctg
     1621 tcaacaatct tagctacact aaagaacgtg cggagaaata cctctatgcc gcaccaattg
     1681 cccaaaatcc taatgtcctt gtagtgaaga aagatgactc tagtatcaag tctctcgatg
     1741 atatcggtgg aaaatcgacg gaagtcgttc aagccactac atcagctaag cagttagaag
     1801 catacaacgc tgaacacacg gacaacccaa ctatccttaa ctatactaag gcagatttcc
     1861 aacaaatcat ggtacgtttg agcgatggac aatttgacta caagattttt gataaaatcg
     1921 gtgttgaaac agtgatcaag aaccaaggtt tggacaactt gaaagttatc gaacttccaa
     1981 gcgaccaaca accgtacgtt tacccacttc ttgctcaggg tcaagatgag ttgaaatcat
     2041 ttgtagacaa acgcattaaa gaactctaca aagacggaac gcttgaaaaa ttgtctaaac
     2101 aattcttcgg agacacttac ctaccggcag aagctgatat taaataattt cttgaaatca
     2161 tccattctga gtaagatggg tggtttctca gtttttaggg atatagtttt aaactatata
     2221 tctgactatc taataacaat ttgtgaaatc aaacaaattg tgagatacta gtacggtatt
     2281 atttttaagg agaaagaatc atgaaaatca aaaaatggct tggcctagca gcccttgcta
     2341 cagtcgcagg tttggctctt gcagcttgcg gaaactcaga aaagaaagca gacaatgcaa
     2401 caactatcaa aatcgcaact gttaaccgta gcggttctga agaaaaacgt tgggacaaaa
     2461 tccaagaatt ggttaaaaaa gacggaatta ccttggaatt tacagagttc acagactact
     2521 cacaaccaaa caaagcaact gctgatggcg aagtagattt gaacgctttc caacactata
     2581 acttcttgaa caactggaac aaagaaaacg gaaaagacct tgtagcgatt gcagatactt
     2641 acatctctcc aatccgcctt tactcaggtt tgaatggaag tgccaacaag tacactaaag
     2701 tagaagacat cccagcaaac ggagaaatcg ctgtaccgaa tgacgctaca aacgaaagcc
     2761 gtgcgcttta tttgcttcaa tcagctggct tgattaaatt ggatgtttct ggaactgctc
     2821 ttgcaacagt tgccaacatc aaagaaaatc caaagaactt gaaaatcact gaattggacg
     2881 ctagccaaac agctcgttca ttgtcatcag ttgacgctgc cgttgtaaac aataccttcg
     2941 ttacagaagc aaaattggac tacaagaaat cacttttcaa agaacaagct gatgaaaact
     3001 caaaacaatg gtacaacatc attgttgcaa aaaaagattg ggaaacatca cctaaggctg
     3061 atgctatcaa gaaagtaatc gcagcttacc acacagatga cgtgaaaaaa gttatcgaag
     3121 aatcatcaga tggtttggat caaccagttt ggtaataaga aacagggagg tgggagagag
     3181 aattccacct cttgcttttg tatagagcat ggagtgtaaa gaagactatt cgttcataga
     3241 aaggtagaga gaatatggtt tttcctagcg aacaagaaca gattgaaaaa tttgaaaagg
     3301 atcatgtagt ccaacattat tttgaggttt tgcgtacctt gatttctaag aaatcagtct
     3361 ttgcccagca ggttggactc aaggaagtcg caaattatct gggtgagatt ttcaagcgtg
     3421 ttggagctga agtggagatt gatgagagct atacagcgcc ctttgtcatg gcacatttca
     3481 agagttcgcg tccagatgcc aagaccttga ttttctataa ccactatgac actgtgccag
     3541 cggatgggga tcaggtctgg acagaggatc cttttacgct ttcggtccgc aatggcttca
     3601 tgtatgggcg tggggttgat gacgacaagg gtcatatcac agctcgcttg agtgctttga
     3661 gaaaatatat gcagcgccat gatgatttac ctgtcaatat cagctttatc atggagggag
     3721 cggaggaatc ggcttcaaca gacctagata agtatttgga aaagcatgca gacaaactcc
     3781 gtggggcgga tttgttggtc tgggaacaag ggaccaaaaa tgccttggaa caactggaaa
     3841 tttctggtgg caataagggg attgtgacct ttgatgccaa ggtaaaaagc gctgatgtgg
     3901 atatccactc gagttatggt ggcgttgtgg aatcagctcc ttggtatctc ctccaagcct
     3961 tacagtctct tcgtgctgcg gatggccgta tcttggttga aggcttgtac gaagaagtac
     4021 aagagcccaa tgaacgagaa atggccttgc tagaaactta tggtcaacga aacccagagg
     4081 aagttagtcg gatttatgga ttggagttgc ctctcttaca ggaggatcgg atggcctttc
     4141 taaaacgttt ctttttcgag ccagcgctta atatcgaagg aatccagtct ggttatcaag
     4201 gtcagggtgt taagactatt ttacctgcag aagccagtgc caagctagag gttcgtctgg
     4261 ttccgggcct agaaccgcat gatgttctgg aaaaaattcg gaaacagcta gacaaaaatg
     4321 gctttgataa ggtagaatta tactatacct tgggagagat gagctatcga agcgatatga
     4381 gcgcaccagc cattctcaat gtgatcgagt tagccaagaa attctatcca cagggcgttt
     4441 cagtcttgcc gacgacagcg gggacaggac ctatgcatac ggtctttgat gccctagagg
     4501 taccaatggt tgcattcggt ctaggaaatg ccaatagccg agaccacggt ggagatgaaa
     4561 atgtgcgaat cgctgattat tacacccata tcgaattagt agaggagctg attagaagct
     4621 atgagtagag atattatcaa gttagatcag atcgatgtga cttttcacca aaagaagaga
     4681 accatcacag cggttaagga tgtgaccatt cacatccaag aaggggatat ctacggaatc
     4741 gttggatatt ctggagcagg gaaatcaacc cttgtacggg tgattaatct cttgcaaaaa
     4801 ccatctgcag ggaaaattac cattgacgac gatgtgattt ttgatggtaa ggtcaccttg
     4861 acggcagagc agttgcgtcg taaacgtcaa gatatcggga tgattttcca gcattttaac
     4921 ctgatgagcc aaaagacagc agaggagaat gtagcctttg cccttaaaca ctctggactc
     4981 agcaaggaag aaaagaaggc taaagtagct aagttgttgg acttggttgg tttggcagat
     5041 cgtgctgaaa actacccttc acaactatct ggagggcaaa aacagcgtgt ggcaattgcg
     5101 cgtgccttgg ccaatgatcc aaaaatcttg atttcagacg agtcaacttc tgcccttgac
     5161 cctaagacaa ccaagcagat tttggccttg ttgcaagatt tgaaccaaaa attaggattg
     5221 acagttgtct tgattacgca tgaaatgcag attgtcaaag acattgccaa ccgtgtggcg
     5281 gttatgcagg atgggcattt gattgaagag ggaagtgtcc ttgaaatctt ctcaaaccct
     5341 aaacaacctt tgactcaaga ctttatctca acagccacag gtattgacga agccatggtc
     5401 aaaatcgaga agcaagaaat cgtggaacac ttgtctgaaa acagtctctt ggtgcaactc
     5461 aagtacgctg gagcttcaac agacgagcca cttttgaatg aattgtacaa gcattaccaa
     5521 gtaatggcta atattctcta tgggaatatc gaaattctcg atggtactcc tgttggagaa
     5581 ttggtggtgg tcttgtcagg tgaaaaagca gcgttggcag gtgcccaaga agccattcgt
     5641 caagcaggtg tacaactaaa agtattgaag ggagtacagt aagatggaat cattgattca
     5701 aacctattta ccaaatgtct ataaaatggg ttgggctggt caggcaggct ggggaacggc
     5761 tatctactta actctttata tgacagttct ttccttcatt atcggaggct tcttggggct
     5821 agtggcaggt ctctttctcg tcttgacagc gccaggtggt gtcttggaga ataaagtcgt
     5881 attctggatt ttagacaaaa ttacctcaat ttttcgtgcg gttcccttta tcatcctctt
     5941 ggcaatcttg tcaccacttt ctcacttgat tgttaaaaca agtatcgggc caaatgcagc
     6001 ccttgtccca ctttcttttg cagtctttgc cttctttgct cgtcaggtgc aggttgtctt
     6061 ggctgaattg gatggcggtg tcattgaggc ggctcaagcg agcggagcga ctttctggga
     6121 catcgtgggt gtttacctat cagaaggtct tccagatttg atccgtgtga cgactgtgac
     6181 cttgatttcc cttgttgggg aaacagctat ggccggtgcg gttggagctg gtggtatcgg
     6241 taacgtagcc atcgcttatg gatttaaccg ctacaatcac gatgtgacca tcttggcaac
     6301 catcattatc attttgatta tctttgcaat ccaattcttg ggagatttct tgactaagaa
     6361 attgagccat aaataaaaaa gagccgcgtg gctcttttta actgatcaga ttttctgtgc
     6421 aaatttttta ctcaaggctt gtccaatcaa ggcacccact agggctccga tgacaatact
     6481 tgcgataaat agaaggacag ttccagggtt tggagcgacc atgatgcggt cgatatattc
     6541 ttgggatttt cctcttgcca gaagagtagc catataggct ttgggcgcaa tccacataag
     6601 caagattggt cctgttgtac taaaggcgaa aataatgaaa gaaaggaagt tctttgtttt
     6661 gtccttgtat tttcctaaat gagctactcc atctgctagg aggccacaga taattccagg
     6721 aaggaaggaa ccagcaccgt gtttagttcc caagaaaaag agggcaatga caaggccgat
     6781 agtggtaatg gctccaaagc gcggaacttt tgcgactagg atcatataga cgctaccgcc
     6841 gacaagggca gtaaaggcag gcgcataaaa catgtttcca gtttggtcaa agagattgcc
     6901 caaaaggaca ccaatcccca tgcagaggaa gtaaagaact gcaaaaagca gtgtagttaa
     6961 gatagatttc ttcatgaatt gtctcctgat aattttttca caattctcat tttaccatgg
     7021 tttggtggga ttgtaaatgg atactagaag tttttgaaaa cgcttgaaat atgatataat
     7081 ggtttcatag ttatttttag gggaatatca tgggtagatt tttagacttt gtctttaatc
     7141 gtttcttttt agggatgatt gcgacagcct tcttttggtt attaacttta gcaggaggga
     7201 ttatccttgg tctagcgccg gctagtgcca ccttgatgag cttatatgca gaacatggtt
     7261 atagctttcg ggaatacagt ttgaaggagg cttggtcttt ttacaagcaa aattttgtct
     7321 caagcaacct gattttctat agctttttag gtgtgggtct agttttgacc tatggtttgt
     7381 atctcttggt gcaattgcct catcagacca ttgttcattt gattgcgacc tttttgaatg
     7441 tcctagtagt tgccctgatc tttttggctt atacagtatc tttaaaatta caagtttatt
     7501 ttgccttgtc ctatcgaaat agtctcaaat tatccttgat tggcatcttt atgagtctag
     7561 cagctgtggc caaggttctc cttgggactg tgctacttgt agcaattggt tactatatgc
     7621 ctgctcttct tttctttgta ggaattggga tgtggcattt ctttatcagt gatatgttgg
     7681 aaactgtcta tgaaatcatc catgaaaaat tggcgacaaa atagaatgaa gcagttttgg
     7741 ctacatacgc ttctaagaac ctatagttca gtgatgatca ttatcattgc gagttttgca
     7801 atcttactct cttacgctga ctgggattca cgtgaaaagg aagcccagag agtagcccag
     7861 cgtgtaactg ctaattgaat attaccatag agagtcaacc cagatagctc agactttagt
     7921 tgaaaatcaa gctcgtattg agggaatcta taaatacttt agccttagca tgccagacta
     7981 tttttactgg caattagagc ggaaagcttc gccttatata tcagtctctc tgtatgaaaa
     8041 tgttgatgac ctctatgttc gaaatgattt tgtaactgga gtggccatcg cttttcaaga
     8101 ttacaaggaa gtctatgttt ctactaaaga caaacgtagt ggagaaaaaa taagggctga
     8161 ggatttcaaa ccagcaggaa atagttttgc cattccgatg tcagatccag tgtcagatca
     8221 agatttagga gtgatttaca tctccttgga tcctgctgtt ttataccatg ccattgataa
     8281 tactagaggt catactccga tggcagtaac agtgacctca ccttttgata cggagatttt
     8341 tcatattggt gagacagttg ataaggagag tgaaaattgg ctcgttggct taacacctca
     8401 tggctatcag gttcaggtgg cagttcctaa aaactttgtt ttacaaggaa cagtgactag
     8461 ctctgctttg attgtgggtt tgagccttct ctttattgtc attctttatc tgactttgag
     8521 gcatactttt gctaattacc aaaagcaggt agtggattta gtagaatcca ttcaaatcat
     8581 tgctcaaggc gaagaggggc gtcggattga catttccgaa aaagatcagg aattactcct
     8641 aatcgcggag acgaccaatg atatgttgga tcgattggaa aagaatatcc atgatattta
     8701 ccagttagag cttagtcaaa aagatgccaa tatgcgagcc ttgcaggcgc aaatcaatcc
     8761 tcattttatg tataatacgc tggagttctt gcgcatgtat gcagttatgc agagtcaaga
     8821 tgagttggca gatatcattt atgaattcag tagtctcttg cgtaacaata tttccgacga
     8881 aagagagacc ctcctcaaac aggaattaga attttgccgt aaatacagct atctctgcat
     8941 ggttcgctat cccaagtcca ttgcctatgg tttcaagata gatccagagt tagagaatat
     9001 gaagattccc aagtttacct tgcaaccgct ggtagaaaac tatttcgcgc atggtgttga
     9061 ccacaggcgg acagataatg tgattagcat caaggctctt aaacaggatg cttttgtgga
     9121 aattttggtg gtcgataatg gtagaggaat gtcggctgaa aagttggcaa atatccgaga
     9181 aaaattaagt cagagatatt ttgaacacca agccagctac agtgatcaaa ggcagtctat
     9241 cgggattgtc aatgtacacg agcgttttgt gctctatttt ggagaccgct atgccattac
     9301 gatagagtct gcagagcaag ccggtgttca gtatcgtatt acaattcaag atgagtagaa
     9361 agggagaaaa tgtataaagt attaatagta gatgatgagt acatggtgac agaaggtctg
     9421 aaacgtttga ttccctttga taagtgggat atggaggtcg tcgcaacagt cagtcatgcc
     9481 gatgaagctc tagaatatgt tcaggaaaat cctgtcgatg tcatcatttc cgatgtcaat
     9541 atgccagaca aaacagggct tgatatgatt cgggagatga aagagatctt accagatgct
     9601 gcctatatcc tgctctcagg ttatcaggag tttgattatg taaaaagagc aatgaacctt
     9661 agtgtggtgg gctatttggt caagcctgtt gataaggtag agctgggaaa tctgctggag
     9721 aagattgcag gtcagctcgg cgagagaggg aagaaaagtc agactcttag tcaagaatta
     9781 gacggggctg gatttgttag ttatttaggg gataaggaga attggtggat aggtctatcc
     9841 aaggaaaaac aaggttcctt caccactccc tactatgtct tgggtcaaga ctggcagatt
     9901 ttcatttctg accaacccct agatggttta gtcgttacac cttttgaagc tccttatcaa
     9961 gaacactttg aacgctggaa gctgaatgct gagaaagccc tcttttacgg ctctgtaaat
    10021 ctgcagcagt ctgagagtct ctttgcctat tacgaaccga tttatagggt tatcattcag
    10081 ggaaatctca atcaaatcgt agaagagtta aatctcttgg agaaggtagt tcttgaaaat
    10141 acgccgcgtg ttccgattac taaacagctt tttattcagt ttgtcatgga tgttttccat
    10201 ttatttggac atctcaaagc tgatgacctg acagacatcg tcaaaaccat tcatgctatt
    10261 caatccttcg atgaattggt tccttatatc aagggaactc tgaccagctt tttcggtcaa
    10321 taccgtatga atgaaaatgt ggtcagtgtg ctggaagtta ttgggcgtga ttatcagaaa
    10381 gaactttcca tcaaggatat cagtaaggat ctctttatca atcctgtcta tctagggcag
    10441 tcgattaagc gagaaaccga ttcgactttt gcagagttac taaacaaaca acgtattaag
    10501 gctgcccaac aacttttgct ttcaactagt gatagcatcg aagatatttg ttatgctgtt
    10561 ggttacagta accttggata tttctataaa gttttccgaa aattgtgcgg aaaattgcca
    10621 aaagcctacc gaaaacaggt agaaactata ctataagctt tgtattcctt tacaaaaggt
    10681 gctataatat aaataatatt atcaggaggt tctatcatga aaaagaaacc aatttatcta
    10741 tgggtcttgc taatcttgtc tgcccttatt tcagtttcgt ctctgtttgg aattgtgagt
    10801 cccttgccta gtaaagaagc ccttcgtgct gctcagaaac aagttgcagg ggtcaatgct
    10861 cagcaattag aagaccagct taattatacc tatagagtag cagaagcatc tcattctatt
    10921 tttaatgttg ctttgattgt gctatctact attttagtgg tcgtagcgat tgtcttcctt
    10981 gttcgtaaaa atttgcaata tgcaaactat acttacgttg gctatgtttt gttagctatt
    11041 attggctcta tttatggcta tgttggttta caagacgctg tgcagttggt tcaagatgag
    11101 agcatgcgtt tgacagtgag tattggttca aaagctgtta gcattttcta tatcgtcatc
    11161 aatgttctct tcctagccct tgtcttctat aagatgtggc gacaacagaa agccttggca
    11221 gaagaagagg aaacagaaga gcttacctaa gtattgacaa aaaagaacta tcaagataca
    11281 atcttggtag ttgttttttg attatagata aaatcatgga ggtacatatg aagacaattt
    11341 ctttagttta tattagtttg agtggcaaca ctgaaagttt tgtgacgcgc ttgaaagact
    11401 atctcttgtc ccagtacaaa gggattgagg ttcaaaagat tcatatcaag gatttggtca
    11461 aggaaggcaa aaatttctat gaaatggacc atccctatgt cgcttttttg ccgacctatc
    11521 tcgaaggtgg gaatggcgtg gataacggag atgttgagat tttgacgaca ccggtgggag
    11581 attttattgc ctatggtaac aatgctagta agtgttttgg tgtggttggg tcaggaaatc
    11641 gtaactttaa taaccaatac tgcctaacag ccaagcaata cagtcaacgc tttggcttcc
    11701 ctgtattggc tgactttgaa atgcgaggca tgctggaaga tatcaaatat gtcgcagcta
    11761 ttatcgcaga tttgtatgag ttggaaaagg agaattaata ctcaatgaaa atcaaagagc
    11821 aaactaggaa gctagtcgca ggttgctcaa agcacagctt tgaggttgca gatagagctg
    11881 acgtggtttg aagagattct cgaatagtat aaagttctta taagaggtgg ttgaagttgt
    11941 tcaactgttt tttgagtata aacagtcttt aagaacgtaa aaaagcataa gttcaggtct
    12001 tcaaaacctt gttcttatgc tttttatcat agaaatattt gctgaatttt ctcttgaaat
    12061 gaaaccttat cttcatctcg cgcttgtccc ttaccctgct tttccgattg cgagtgcgta
    12121 gataaagaag atggcgaagc caaagaggaa aatcaatccc gcaatggcaa ggtgtttgag
    12181 ccaagaagga agatttttgt tttcacgcgt taccaaggcg taattcaaaa gagcaaagaa
    12241 aggtgttgtc aggaaagagc caatcatggc aaagcggagc atggttgaaa cctgaccagc
    12301 gaagaacttg ataatgacga taccgatgat agcagtgatg gtcatccaga tgttcaaaga
    12361 tttacgattg tcctcttttt gactgattag cagtcggaga gattcctgat taacgcgaga
    12421 atagccatcg ataacagtta taactgttcc aaagatacag aggaaggcaa taaaggtaat
    12481 caagtaacgg gaccattcgc caagaacaga ggcatacatg cccacgaatt gagagatgta
    12541 tttggctgaa gcagcttcaa ccgcctgccc tgtaggatac tgaatcagtg ctcccagtgc
    12601 cacaaagaag acggctagga tagctgttcc aatataacca gtgttaaagt caaacagagc
    12661 gtcttctgtg ttaaagttga cggtctttct cttttcagct gaccaaagtg aattgatggc
    12721 tgaaatttca ataggagccg gcatccatcc taggagggag acgatgaagg gcagagctgc
    12781 catttgccaa ggtgtcttct cgacaaaatc agaactgtat tctggatgct tgaccgccgc
    12841 aatgataact gcaagaacag tcgcaatggt taaggcagac ataatccatt tgaccatgcc
    12901 gtctaaaagt ttgtagcctc caaagagtag catagcccaa atgattgcaa cgagaatgag
    12961 ggaccactga gtaatactaa gtccaatcat tgggaaggca ctggcgatga tagctgagca
    13021 cagaatggca acaccagccg tgttgaccat agccgaaaag acattgagga taaagaaaat
    13081 ccagagatag agttttcctt tttcggcata accttcaacc aaagtctttc cagtatcagc
    13141 tgtgtattca gcaccaaaac ggaaaaatgg atatttaaag acattggcta agatgaccaa
    13201 gagaagtaga gaccaaccgt aagaaccgcc agcttgagtt gaggatacaa tgtgggaacc
    13261 tccaacagcg gcagttgcca ttaggattcc aggtcccatt gcttttagct tgcttgccca
    13321 ggttgattga tttaaggaaa tagcttgcga catgataaat actccttttt gaattttcag
    13381 aataatctgt atatgtaatc tatattctac aaaaaatttt ggaaaaatgc aagaatattt
    13441 tgaaaaaaga tagaaaattt acaaagaaaa tctgaaataa ctgttatgat atagtagatt
    13501 gaaactagaa tagtacacct ttgcttctaa aacattgtta gaaatcgatt tgactgtcct
    13561 gatcgattta tcctgttctt atttcatttc actatagtaa ggtgcagcag gataaagaaa
    13621 aaccgagaag tttctttctc ggatttgctt attatgaggt tcctattttc tatgctccaa
    13681 gagttgattg atgtgatcta cttttttatc ctctctttta tagagaatag cccagatgat
    13741 gagatagaca atcgcaaact cgataatgag ctggaggtag aaaatccagt ggaaagggaa
    13801 ccaaccagca agagttgcta gtgggacaaa gccagccagc ataagtaaga aatgagtcag
    13861 agtggcacga agcatgctcc agtcacggct gaataagcgg ttgccaaagt tgaagagcat
    13921 accgatggct gcccagataa gtgtgcagta gagcaagacc agggcaccgt gaacctgatg
    13981 ctgatccatc acttggccga tgagagagta gggatttagt ggtgcgtagg tatttggtgc
    14041 ataaatgagt gaaaagagga tggagaggat gaggccgata agaacaccgg cagctgcatc
    14101 gtgaaatact tgttttttca tagttctaat ttctccttga tggtttttag ataacggcgt
    14161 gaagagtagg tgaagctttc gtttttcaag aaaatttcta ccagaccgtt tggcgtgagc
    14221 ttgaggtgag agatggaatc gatattgatg atttctgatt gggaaatttg gataaaattg
    14281 cttggcaaga gtttaagaac ctgatagagt cgcaaatcaa tgctgtaggt ctgactcgcg
    14341 gtttctgcta gaaccttccg attctcgata tagaagcgct gaatcttgcc aatctcaact
    14401 agatagacct gatcatcgat tttccctttg attttttctc tttggtccag attttctgcg
    14461 aactcgatga ctttttggac tttatctgtc ggctgaggtg cttggacaat cagcttttcc
    14521 tcctcgtaag tctcactaat ctgtagttct actttcataa atttctctcc ttgtttttca
    14581 tacaaaatta atctcacttc ttgtacacct ttattaatac tcttcaaaaa tctcttcaaa
    14641 ccacgtcagc gtcgtcttgc cgtaggatag ttgttgactt tgtcagtttt atctacaacc
    14701 tcaaaacagt gttttgaact gtctgagcct agctttctag tttgctcttt gatttttatt
    14761 gagtatacca ctattttact ccctctggca agggactttg tctatgtgga gggattgggc
    14821 tcctatgtgg tggagctttt ctgttctttc tgaaatatgg tataatagca ctaattaatt
    14881 tctaggaaaa tagatacaga aaggggctga aagatgtctc atattattga attgccagag
    14941 atgctggcaa accaaatcgc agctggagag gtcattgaac gtcctgccag tgtggtcaaa
    15001 gagttggtag aaaatgccat tgacgcgggc tctagtcaga ttatcattga gattgaggaa
    15061 gctggtctca agaaggttca aatcacggat aacggtcatg gaattgccca cgatgaggtg
    15121 gagttggccc tgcgtcgcca tgcgaccagt aagataaaaa atcaagcaga tctctttcgg
    15181 attcggacgc ttggttttcg tggtgaagcc ttgccttcta ttgcgtctgt tagtgtcttg
    15241 actctgttaa cggcggtgga tggtgctagt catggaacca agttagtcgc gcgtgggggt
    15301 gaagttgagg aagtcatccc agcgactagt cctgtgggaa ccaaggtttg tgtggaggat
    15361 ctctttttca acacgcctgc ccgtctcaag tatatgaaga gccagcaagc ggagttgtct
    15421 catatcattg atattgtcaa ccgtctgggc ttggcccatc ctgagatttc ttttagcttg
    15481 attagtgatg gcaaggaaat gacgcggaca gcagggactg gtcaactgcg ccaagcaatc
    15541 gcagggattt acggtttggt cagtgccaag aagatgattg aaattgagaa ctctgaccta
    15601 gatttcgaaa tttcaggttt tgtgtcttta cctgagttga ctcgggctaa ccgcaattat
    15661 atcagcctct tcatcaatgg tcgttatatc aagaacttcc tgctcaatcg tgctattttg
    15721 gatggtttcg gaagcaagct tatggttgga cgttttccac tggctgtcat tcacatccat
    15781 atcgaccctt atctagcgga tgtcaatgtg catccaacta agcaagaggt gcggatttcc
    15841 aaggaaaaag aactgatgac tctggtttca gaagctattg caaatagtct caaggaacaa
    15901 accttgattc cagatgcctt ggaaaatctt gccaaatcga ccgtgcgcaa tcgtgagaag
    15961 gtggagcaaa ctattctccc actcaaagaa aatacgctct actatgagaa aactgagccg
    16021 tcaagaccta gtcaaactga agtagctgat tatcaggtag aattgactga tgaagggcag
    16081 gatttgaccc tgtttgccaa ggaaaccttg gaccgattga ccaagccagc aaaactgcat
    16141 tttgcagaga gaaagcctgc taactacgac cagctagacc atccagagtt agatcttgct
    16201 agcatcgata aggcttatga caaactggag cgagaagaag catccagctt cccagagttg
    16261 gagtttttcg gacaaatgca cgggacttat ctctttgccc aagggcgaga tggactttac
    16321 atcatagatc agcacgctgc tcaggaacgg gtcaagtacg aggagtaccg tgaaagcatt
    16381 ggcaatgttg accagagcca gcagcaactc ctagtgccct atatctttga atttcctgcg
    16441 gatgatgccc tgcgtctcaa ggaaagaatg cctctcttag aggaagtggg cgtctttcta
    16501 gcagagtacg gagaaaatca atttattcta cgtgaacatc ctatttggat ggcagaagaa
    16561 gagattgaat caggcatcta tgagatgtgc gacatgctcc ttttgaccaa ggaagtttct
    16621 atcaagaaat accgagcaga gttggctatc atgatgtctt gcaagcgatc tatcaaggcc
    16681 aatcatcgta ttgatgatca ttcagctaga caactcctct atcagctttc tcaatgtgac
    16741 aatccctata actgtcctca cggacgtcct gttttggtgc attttaccaa gtcggatatg
    16801 gaaaagatgt tccgacgtat tcaggaaaat cacaccagtc tccgtgagtt ggggaaatat
    16861 taaaagtata aaaaagtctg ggaaaaatgt tcaaaatcag aaaaacgcat aaaatcaggt
    16921 gttcaaaaac cttgatttta tgcgttttat catagaaata tttacttcat gttttcctaa
    16981 ttcttttcga aactcttttt aaaccacgtc agttttatca gtaatcttaa aacagtgttt
    17041 tgagctaatt ttgccagttt tgtctgtaac atcgaagttg tgtttgacca ctctgcgact
    17101 ggtttcctag tttgctctat gattttcaca gagtattaaa ttgcgatttt gccaagtttc
    17161 tttattcgtc taaaagtaga gtctgttcta tgcgtctaat gtacgaatta ggttgaccat
    17221 ttcaatagct ccttgtgcac actcagaacc cttatttcct gctttagtgc cagctcgttc
    17281 tatggcttgt tcaattgtat ctgtcgttag cacaccaaac ataacaggaa tttcgctatt
    17341 taaactgatt tgggcgattc ccttagatac ctcgctacat acataatcat aatgacttgt
    17401 attccctcta atgacagctc ctaagcagat aattgcatca tattttttac tttttgccat
    17461 ttttgatgca atcagtggta tttcaaaagc tcctggaatc caggctacct cgatatcttt
    17521 ctcgtttaca ttctctcttt tgagattatc tagtgctcca gataataatt ttgaagttat
    17581 aaattcatta aatctcgcta caacaatacc tattttaata ttgtttgcta ctaaattacc
    17641 ttcataagtg ttcatttatt tttcctccat atttaaaatg tgacccattc gatttttctt
    17701 tgtttctaaa taaaaactat cgtaagcatt ggcttctatt tcgattgata ttctactgga
    17761 aatggtaatt ccatattttt ctaactgttc aaccttgtca ggattatttg tcagtaaatg
    17821 aagtgactga agtcccagat ctttaagtat ttgtgctcca atatggtatt ctcttaaatc
    17881 accttcaaag cctaatgcaa gattggcatc aagcgtatcc atgccttgat cttgtaaatg
    17941 ataggctttt aatttattga taagtccaat tcctcgtccc tcctgtcgca agtaaagtaa
    18001 gacacccgaa ccattctcaa caatcatttt catagcttta tcgaattgct gtccacaatc
    18061 gcaacgtaaa gagcctaaaa catctcctgt taaacattcg gagtggaccc gacataatac
    18121 attggcttca tcctctatat ttcccataat aagagcaaga tgatgttccc catttagttt
    18181 atctatatag ctaattgctt tgaaattacc gtatctagta ggcatattga cagttgaaac
    18241 tcgttctacc agctgatcat atacttttct atattcttgt aattctttga tggtaattag
    18301 tggaatgttg tgtttcttcg agaactgaat taaatcatct gttctcatca ttttgccatc
    18361 atgattcatt atttcacaac ataggccaca ctcttttagt ccagctaatt ttaataaatc
    18421 aacagttgct tctgtgtgtc catttctttc tagaacacca cctttttttt caattaaagg
    18481 aaacatgtgt cctggcctgc gaaaatcaga gggtgttata tcttcagcta cacacatacg
    18541 tgcggtcagt cctctttcct cggcagaaat acctgtggtc gtttctttat aatcaattga
    18601 aactgtaaaa gcagtcttat gattatctgt attgttttca atcataggtg aaagcattaa
    18661 ttgattagct aaactttcgc tcataggcat acaaattaat cctttggcat aagtagccat
    18721 aaaattaaca ttttctgttg tagctgcttg tgcagaacaa attaagtctc cttcattttc
    18781 tctatcctta tcgtctataa caagaacaag tcgtcccttc tgcaatgctt ctaatgcttc
    18841 ttgtattttt cgatattcca ttgactgatt atcctttctg ctaaaatcca ttttgatata
    18901 atagttcctt agatatttct gattttggag aattatccat cagtttttgc acatatttac
    18961 ctaagatatc attttcaaga tttactgtac tcccgacttg tttactctta agaatggttt
    19021 gttcccaggt atgagggata acagatactg aaaagtttac tttggagact ttagcgacag
    19081 tcagactaat gccgtcaatt gtaatagatc ctttttcaac tattaaatct aaaatttctt
    19141 tttgtgtgtt gatttgatac catacagcat tatcatcttt ttttattgac gagatttttc
    19201 ctgtaccatc aatgtgtcct gtaacgacgt gacccccaag tcgaccgttg acagataagg
    19261 ctctttctag attcacctca cttccatgtt ttaatagagt aagagctgtt cgactccatg
    19321 tttcattcat tacatcaact gtaaaggatt gatgattgaa atgagtaact gtaagacaga
    19381 taccatttac tgctatacta tcacctaaat ggatatccgt taatattttt gaggctttaa
    19441 ttgatagttt acaattacga gagtctttct gtattctttc aacttttccg atttcttcaa
    19501 ttattcctgt gaacatggat aaatcacttc actttctatg agatagtcat ttcctatttg
    19561 agaaaaagca taaggtttca atctaatagc gtcatttggc aaagaaatgc cttcacctcc
    19621 gataggaaac ttggcactgc ctccaaaaat ttttggtgca atatatattt tcagctcatc
    19681 aacaatttgt tgttccaaag cactccaatt cattagactg cccccttcta gaactaggct
    19741 atcaatctgc atgtttccta gatgttgcat taaactcgat aagtctatat gattgccttt
    19801 tttctttatg gaaagtattt tacagccatg attttgatat agcttcattt tatttttgtc
    19861 ttcagaggaa gtggcaatgt aagttttaat atcatttgct gtttttacga ttttagaggt
    19921 aagaggagtt cgtaaatgtg tatcgcatat gatacggata ggatttttcc cttcctccaa
    19981 tctacatgtc agcaaaggat cgtcttgaat aacagtgttg actcccacca taattgcact
    20041 aacatggtgt cgtaactgat gcacatgctt tcttgcttct tcttcagtaa tccatttgga
    20101 ttgatttgtt ttagtggcta tttttccatc cattgacatt gcatatttca taaaaacata
    20161 gggtacatgc tgggtaatat actttctaaa actttttatt aagttaagac actcattttc
    20221 taaaattcca acagtaactt gaagattatt ttcctcaagt atctttactc cttttccaga
    20281 tacaatagga ttacagtcta ggcttccaat gactactctt gtaataccac tatcgattat
    20341 agcatctata cagggaggtg ttttcccgaa gtgacaacag ggttcaagtg ttacataaag
    20401 cgtcgctccg acaggggatt ctctacagtt tttaagagca tttctctcag catgtgggcc
    20461 accaaaaaac tcatgataac cttgtccgat gatgtgatta tcttttacaa taactgcgcc
    20521 gaccatagga ttggggttga cgtaaccagc ccctttttgt gccagtttta ttgctaattt
    20581 catatatttt gaatcgctca tctcactacc tccaaaaaat ataccttgaa taggggacta
    20641 ctcaaggcat acaaaagaaa acttatgcga ttaacaaaaa tgctctgaaa tgacaagtaa
    20701 tcatttcaga gcacgcaaaa agcacaaata tacttttatc ttctttcatc cagactatac
    20761 tgtcggcttt ggaatttcac caaatcatgc ctttcggctc gtgggctata ccaccggtag
    20821 ggaattgcac cctgccctga agatagttat tcaattacag atgattatag tacttaattt
    20881 tgaatatgtc aacagataaa taccgattgt ttttgatata ctgtatttgt gataatcgat
    20941 tctcgctcct cggataaaga aaatatgata tactagataa acgaaataag agagaaggaa
    21001 t
//
LOCUS       DAVKDZ010000029        20885 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000029 DAVKDZ010000000
VERSION     DAVKDZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20885
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.030"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(193..2190)
                     /gene="metG"
                     /locus_tag="VWZ30_001093"
     CDS             complement(193..2190)
                     /gene="metG"
                     /locus_tag="VWZ30_001093"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU9334850.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPEGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEVVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(2314..3573)
                     /locus_tag="VWZ30_001094"
     CDS             complement(2314..3573)
                     /locus_tag="VWZ30_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9334851.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(3575..4276)
                     /locus_tag="VWZ30_001095"
     CDS             complement(3575..4276)
                     /locus_tag="VWZ30_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9334852.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(4260..5459)
                     /locus_tag="VWZ30_001096"
     CDS             complement(4260..5459)
                     /locus_tag="VWZ30_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU9334853.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            5745..7091
                     /gene="gor"
                     /locus_tag="VWZ30_001097"
     CDS             5745..7091
                     /gene="gor"
                     /locus_tag="VWZ30_001097"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU9334854.1"
                     /translation="MKEYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RINLPLHTHKVPAKLEKTAKGITIHFEDGTSHTASQVIWATGRRPNVKSLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTVKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            7288..7824
                     /locus_tag="VWZ30_001098"
     CDS             7288..7824
                     /locus_tag="VWZ30_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU9334855.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            7875..7961
                     /locus_tag="VWZ30_001099"
                     /pseudo
     CDS             7875..7961
                     /locus_tag="VWZ30_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(8030..8245)
                     /locus_tag="VWZ30_001100"
     CDS             complement(8030..8245)
                     /locus_tag="VWZ30_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEU9334856.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            8343..8663
                     /locus_tag="VWZ30_001101"
     CDS             8343..8663
                     /locus_tag="VWZ30_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334857.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(8702..9031)
                     /locus_tag="VWZ30_001102"
     CDS             complement(8702..9031)
                     /locus_tag="VWZ30_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334858.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVECATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFE"
     gene            complement(9043..9762)
                     /gene="trmD"
                     /locus_tag="VWZ30_001103"
     CDS             complement(9043..9762)
                     /gene="trmD"
                     /locus_tag="VWZ30_001103"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU9334859.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(9752..10270)
                     /gene="rimM"
                     /locus_tag="VWZ30_001104"
     CDS             complement(9752..10270)
                     /gene="rimM"
                     /locus_tag="VWZ30_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU9334860.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(10316..11089)
                     /locus_tag="VWZ30_001105"
     CDS             complement(10316..11089)
                     /locus_tag="VWZ30_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9334861.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAII"
     gene            complement(11666..11905)
                     /locus_tag="VWZ30_001106"
     CDS             complement(11666..11905)
                     /locus_tag="VWZ30_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU9334862.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(11925..12197)
                     /gene="rpsP"
                     /locus_tag="VWZ30_001107"
     CDS             complement(11925..12197)
                     /gene="rpsP"
                     /locus_tag="VWZ30_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU9334863.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            12367..12576
                     /locus_tag="VWZ30_001108"
     CDS             12367..12576
                     /locus_tag="VWZ30_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334864.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERDLDTWLLSPKPVPKRNMDL
                     LADDSLAGDIILLWRIQFGTFTTET"
     gene            13055..13276
                     /locus_tag="VWZ30_001109"
     CDS             13055..13276
                     /locus_tag="VWZ30_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334865.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            13359..14162
                     /locus_tag="VWZ30_001110"
     CDS             13359..14162
                     /locus_tag="VWZ30_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU9334866.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITVDSIEVVKDYDFKS"
     gene            complement(14218..15759)
                     /locus_tag="VWZ30_001111"
     CDS             complement(14218..15759)
                     /locus_tag="VWZ30_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9334867.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERHQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKCALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(15761..16243)
                     /locus_tag="VWZ30_001112"
     CDS             complement(15761..16243)
                     /locus_tag="VWZ30_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9334868.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPDIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(16243..17328)
                     /gene="rlmN"
                     /locus_tag="VWZ30_001113"
     CDS             complement(16243..17328)
                     /gene="rlmN"
                     /locus_tag="VWZ30_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU9334869.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRTDQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERVSHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHIMVSTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            complement(17349..17879)
                     /locus_tag="VWZ30_001114"
     CDS             complement(17349..17879)
                     /locus_tag="VWZ30_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEU9334870.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            complement(18035..18640)
                     /locus_tag="VWZ30_001115"
     CDS             complement(18035..18640)
                     /locus_tag="VWZ30_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU9334871.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(18813..19850)
                     /locus_tag="VWZ30_001116"
     CDS             complement(18813..19850)
                     /locus_tag="VWZ30_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9334872.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKAFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(19884..20819)
                     /locus_tag="VWZ30_001117"
     CDS             complement(19884..20819)
                     /locus_tag="VWZ30_001117"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU9334873.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
BASE COUNT         6083 a         4551 c         3833 g         6418 t
ORIGIN      
        1 catgttttga gctgacttcg tcagtttcat ctacaacctc aaaaacatgt tttgagctga
       61 cttcgtcagt tctatctaca acctcaaagc agtgctttga tcaacccgcg gctagcttcc
      121 tagtttgctc tttgattttc attgagtata aataaaaaaa acagaactag tctgaactag
      181 tcctatctac ttttacccaa tcacacttcc atttggtaca gctggatcaa ctgtgagaag
      241 ggttaatttg ccatcatgtt cagctgagag aatcataccc tggctgacat attttttcat
      301 cattttacgt ggtttgaggt tagcaacgat ttgaactttc ttgccgacca attcttgttc
      361 atttggatag tattttgcaa ttcctgaaag aatctgacga tcttctccat caccagcatc
      421 caagcggaat tgaagcaact tatctgaacc ttccacttta gacacttctt tgacttctgc
      481 gacacggatt tcaaccttgt caaagtcttc aaacttgatt tcatccttgt ttagtttgag
      541 ctcaacttcg tccggattcc attctttttc gactgctggt ttattgcctt ccatttgttc
      601 cttgatatag gcgatttctt cttccatatt tagacgtgga aagataggtg ttcctttggc
      661 aactacagtc acatctgctg ggaagtcagc caaactcaag ttttcaagac tagaaacttc
      721 ttccaaacca agttgagtca aaactgcacg actagtttcc atcataaatg gttcaatcaa
      781 gtgagcaact acacgaatgc tggctgccaa gtggctcatg acacttgcca attggtcacg
      841 aagagcttca tccttggcca agacccatgg tgcggtctca tcgatgtatt tattggtacg
      901 agagatcaga gtccagactg cttcaagcgc acgtggatag tcaactactt ccatgtgtgt
      961 atggaagtct gcgattgatt gttctgcaac ctcagcaaga acatgatcaa attcagtcac
     1021 accttctaca taggcaggga tttgtccatc aaagtactta ttaatcatgg aaaccgtacg
     1081 gttaaggagg ttcccaaggt cattagccaa ttcatagttg atacggccga catagtcttc
     1141 aggagtaaag gttccgtctg aaccaactgg aaggttacgc atgaggtagt aacgaagtgg
     1201 atctagtcca taacgctcta ccaacatttc agggtaaacg acattccctt ttgacttaga
     1261 catttttccg tctttcatga caaaccaacc atgggcaatc aaacgatcag gtaatttaac
     1321 atccaacatc ataagaagga ttggccagta gatagagtgg aagcgaagga tgtcttttcc
     1381 taccatatgg aagactgttc cattccagaa cttgtcaaag ttaccatgtt cgtcttgagc
     1441 gtagccaaga gctgtcgcat agttaagaag ggcatcaatc caaacgtaga caacgtgttt
     1501 tggatttgat gggacaggca ctccccatgt aaaggttgta cgagataccg ccaaatcttc
     1561 caaacctggc tcgatgaagt tgcgtagcat ttcattaagg cgaccctctg gcgtgataaa
     1621 ttcaggatga gctttgaaaa attcgaccaa acggtcttgg tatttgctaa ggcgaaggaa
     1681 gtatgattct tcagaaaccc attcaacctc atgacctgat ggagcaatac caccagtcac
     1741 atttccagct tcatcacgga aaacttctgc cagctggctt tctgtaaaga attcttcgtc
     1801 tgatactgaa taccaaccag agtattcacc caagtagata tcatcttgag caagtaagcg
     1861 ttcaaagacc tgtgcgacaa ctttttcatg gtagtcatcg gttgtacgga taaatttatc
     1921 gtatgagata tctagtaatt gccagagttc tttaactcca accgccattc catcaacata
     1981 ggcttgaggt gtaataccag cttcttccgc tttctgctgg attttctgac catgttcatc
     2041 aagacctgtc agataaaata catcgtagcc catcaggcgt ttgtaacgtg ctaggacatc
     2101 acatgcgata gttgtgtagg cagaaccgat atgaagtttc ccagatggat agtaaatcgg
     2161 cgttgtaata taaaaatttt tttcagacat aatttttcct ttccaggcaa atgaaacctg
     2221 tttttctaac acttcattat atcacatttt taatgaattt cgatagggaa atccatacaa
     2281 aaacaagata gacgagtgtc catcttgttg atctcattca taacgaaggg cttcaattgg
     2341 atcaagtttc gatgccttgt tggctggcaa gactccaaaa atcataccaa cactagccga
     2401 aactgcaaga ctaaataggg caactgggat tgatactcca acttctatac cttctattaa
     2461 accttgcagt aacaaacctg ctaaggcagt taaaccactt gcaattgtca agccaattaa
     2521 gccacctaac aaggtcaaaa tcatggattc aatcaaaaac tgaattaaaa tattggcacg
     2581 tgttgcaccc aaagccttac gaagaccaat ctcacgagtg cgctctgtca ccgaaaccag
     2641 catgatgttc atgacaccag ttcctccaac aaagagagaa atccctgcga tggaactaat
     2701 aatcgtcgtc ataaaactaa acgattgttg aatttctgca aatacaacgg actcatctgc
     2761 cacctggtat tctccctgtt gtaagcctgc aagctctgtc atttttcgtg ccagttctgg
     2821 acccagagtt ggggttaaac tggtatcatt cactcgaaag acaatattag ctatttcatc
     2881 tacattaaaa ttcgcagcaa gggagatatt ggtagtaata ggcaagccac caaacccata
     2941 tatttttgat cttttagcct ccggactagt ataaacccca atgacccggt aactaaatcc
     3001 attgacttct acaaccttgt taatagcctc ttgaggagat tcaaataaac taatggacaa
     3061 ttcctcatct agcaaaatga cacttgcaaa ctctttgaaa tcttgctctc tcagactacg
     3121 acctgcaata atttcattct taacagcgtc catgtaagtt ctgtttccac ctgtcaaatt
     3181 agcattctca acctttttat cttgataggt caagatggca ttcgttgaat tggttacata
     3241 gtaactatcc actcccttca gtttagctgc ctcttggacc caggattctt gcggttttgg
     3301 cggttcaaca ggaacttcct cttcctttcc agaaaccgta aaagctgatt gtttctgagt
     3361 aaaagacccg tctttacttt ttttaggaga gaaaaagacg ctaatatttt tctgagattt
     3421 agtcatatct ttattgactt gacgagatag ggaatcaccc aaagccataa tcacaacaac
     3481 tgatgaaaca ccgataataa tcccaatcat agtaagcaaa gaacgcatct tgtgagccat
     3541 gatagatgaa aaggcaaatt tcagattctg catcttagtt ttcctccttt cctaactgag
     3601 cactgtcaga cgaaatgacc ccatcccgaa tgacaatctg acgtttggca taggcagcaa
     3661 tctcaggctc atgcgttacc atgataatgg tttttccttc tttattcaaa tcaaccaata
     3721 attgcataat ttggttacct gttttggtat ccaaggctcc tgtcggttca tccgctagga
     3781 taatagaagg attgtttacc aaggcacgcg caatggctac acgttgcttt tgaccaccag
     3841 ataattctga aggtaaatgg tgactacgtt ctgtcaattc aaccttgtct aaatattcct
     3901 cagccaactt gcgacgtttt gaagacgaaa ctcctgcgta aatcaagggc aattctacat
     3961 tttgcagagc attgagcttc gatagaagaa agaactgctg aaagacaaaa ccgatttgtt
     4021 ggttacggac cttagctagt tgtttttcac caagcccagc cacttcttga ccttcaagat
     4081 aatattctcc actggttggt gtatccaaca tgccaatcgt attcatcaga gtggacttac
     4141 cagacccaga tggtcccatg atggctacaa attcaccctc attcacttct agattgatat
     4201 ttttgagaac ctgcagttct tggtcaccat tacggtaact tctgaagata ttttttagac
     4261 taattagttg cttcatcagc cttcacctct tttccttctt ccaaggaaga tgttggatta
     4321 ctgatgacct tagcaccgtt cgttaaacca gaagtgattt cttgattttc tgcgtcagca
     4381 tttcccaatg aaacctcaac ttttttagcc ttttgttgtt catccacaat ccagacataa
     4441 tttttactat catccattac tagactgcta acaggaacaa gaatagcctt agttttgctt
     4501 ttaacctcaa tgttgacaga aaaaccttgt ttcaaatcac caacctcgcc tgtcacatca
     4561 atagtataag ggtatttaga acctgtatta ttcccggctg ctggactagc tgcttcacca
     4621 ttgtttttag gatagtcaga aatatagctt aatttcccag tccatttttt atcaggatac
     4681 actttagaag taaagcttac ttcttgacct acagaaaggt tggctagatt gtactcagac
     4741 aattctccct tgacttgtaa attttcattg ctgacaatat gaaccataac ttggctcgcc
     4801 cctgttggag atttagaaac attgctattg acttcgacta cagttccctc tagggtactg
     4861 agaacagttg ttgcatccaa ttgactttga gccttgctta attgcgccgc agcatctgca
     4921 cgcgcatcac gggcatcacc caattgagcg tcaatagaag caacagaatt tccagccact
     4981 ggagttgggc tttgcaccgt tgcatcttct cctcctactg gcgctggtaa ctgtggagcc
     5041 ggagctgaag cggcttcatt tcgtgcttga ttgagttcat tgatatgacg atctgcctta
     5101 gctactgctc gactagctga atcataggcc gcctgcgctt ctgaactact gtacttgact
     5161 aaagcctgcc cttcgctgac cttatcgccc acagaaacaa ggatttcatc taaatcaccc
     5221 ttactagcat caaaataaac atattgttca ttttttgctg ttactgtccc tgacaataaa
     5281 acagaggatg ccacgcttcc ttccttggca acaacaagat gagtaggctc atcttttaga
     5341 gcagtctgag aaggttgtct aaagagtaaa atccccccgg cacccaatac aactacactc
     5401 gcagcaccga ttgctgcata cagttgccac tttttagctt taccattctt tttcttcata
     5461 atgaaactcc ttttcttgtt tacaatactt tgctattata ccaaatttcc ctccagcaaa
     5521 caatgcagtt cagatggaaa taatggcagt tcaggattaa acaatcgttc ggaattttgc
     5581 ttttctattg tactagatat ataatacact atcttgactt atttggcaag cttttttgca
     5641 attttttgtg cgtagcagat ttttgcaatc aaatcaaaaa aagaataaaa tataactatc
     5701 aaaaaagacg ctctactatt taaaagatta caaaggagtt ttcaatgaaa gaatatgata
     5761 tcattgctat cggtggaggt agcggaggaa tcgctaccat gaaccgtgct ggtgaacatg
     5821 gagccaaagc agccgttatt gaggaaaaga aattaggtgg aacctgtgtc aacgtcggtt
     5881 gtgttcctaa aaaaatcatg tggtacgggg cgcaaatcgc tgagactttc catcaatttg
     5941 gagaagacta cggctttaag actactgatc ttaactttga ctttgcaacc ctacgtcgca
     6001 atcgtgaagc ctacattgat cgcgctcgtt cttcttatga tggtagtttt aaacgcaacg
     6061 gtgtagactt gattgaaggt catgctgaat ttgtagattc tcatactgta agcgtaaatg
     6121 gtgaactgat tcgtgctaaa catatcgtga ttgctacagg tgcccatcca agtattccta
     6181 atattcctgg tgctgagcta ggtggctctt ctgatgatgt atttgcctgg gaagaacttc
     6241 cagagtcagt tgccattcta ggcgctggtt atatcgccgt tgaattagct ggcgtactcc
     6301 acacttttgg tgtcaagaca gatctctttg ttcgccgcga tcgtccttta cgtggttttg
     6361 attcctacat cgttgaaggt ttggtcaagg aaatggaaag aataaacttg ccacttcata
     6421 ctcacaaagt ccctgccaag ttagaaaaaa ctgctaaagg cattaccatt catttcgagg
     6481 atggtaccag tcacacagct agccaagtta tctgggctac aggtcgccgt ccaaacgtta
     6541 agagcttgca acttgaaaaa gctggagtga ctctgaatga acgtggcttt atccaagtag
     6601 atgaatacca aaatactgtt gttgagggaa tctatgctct aggtgatgta acaggtgaaa
     6661 aagaactgac tccagttgca atcaaggctg ggcgtaccct atctgaacgt ctctttaacg
     6721 gaaaaactac tgtaaaaatg gattactcaa ctattccaac tgttgtcttt tcacaccctg
     6781 ctatcggaac tgttggtttg acagaagagc aagctattaa agaatacggt caagaccaaa
     6841 tcaaggttta taaatcaagc tttgcatcta tgtactctgc ttgcactcgc aaccgtcaag
     6901 aatcccgttt caaactcata acagctggtt cagaagaaaa agttgtcgga cttcatggaa
     6961 ttggctacgg cgttgatgaa atgattcagg gatttgccgt tgctatcaaa atgggagcaa
     7021 ccaaggctga ctttgatgca actgtggcaa ttcacccaac tgcatctgaa gaatttgtaa
     7081 ccatgcgtta atcgaaacaa aagaagctga tataaatatg tctgcttctt ttttaatgat
     7141 tttcgaatca acaaaaaaat tatccaactt gactagtcac gaaaatcggc tgttacagtt
     7201 tttgttacct tgtttttaaa aaatggttga catcgactcc tatatgagta taatagttac
     7261 tatacattag aaaagaggtt aactattttg aaaaaagctc acgtttatgc tatccctgct
     7321 attggggctg ctctcattgc tgttttggca caaatcagtc ttccaattgg acctgttccc
     7381 ttcactctgc aaaactttgc aatcggcttg attgctactg tctttagacc gagagaggct
     7441 gtactttctg ctggactcta tcttcttcta ggtgctatcg gtcttcctgt ctttgcagga
     7501 ggtggagctg gttttcaggc tttagttggc cctactgcag gctatctttg gttttatctc
     7561 gtttactctg gacttacttc ctctctaacc aacagcaaga gtggtgttgt taagattttt
     7621 cttgcaaacc tcttgggtga tgcccttgtc tttgtcggcg ggattctcag cttgcatttc
     7681 ctagctggaa tggcatttga aaaagctctt gttgtggggg ttcttccctt tatcattcca
     7741 gaccttggca aacttctggc tattagtttt attagccgtc ccctacttca acgccttaaa
     7801 aatcaggctt actttactaa ctaaaaaagg atatcgagtt atcatgactc aatatccttt
     7861 tcttttattt tgaaaactta tactcaatga aaatcaaaga gcaaactagg aagctagccg
     7921 caggctgctc aaaacactgt tttgaggttg tggatgaaac tgacgaagtc agtaaccata
     7981 catacggcaa ggcaaagctg atgcagtttg aagagatttt cgaagagtat tagttgcctt
     8041 taaaggcatc caccatcgtt tgaaattctt catttgagag agtaatccct ttgcccattt
     8101 tagtatggtc tggactccaa gcacgaatat caaactttgc aggggcacca ttaaagctca
     8161 cacggttaat ttccttggtc caaccttttt cgttttcaga aagagtcaac aagtgctctt
     8221 cgatttcaaa tgtaaattct gccattttct tctccttttt tagtttcatt agtttattcg
     8281 taaaatcttg tagattttag gaaaatttta tataatattg atataaaaga agggaggcca
     8341 atatgagaca taaattccag caagttctaa ataaaataca tgatttttta aatggatatg
     8401 accaacctga ccagactgaa accaactccc ttacagccac tattgaagag gctatccaga
     8461 aacaaaccgc tgttcacctt atcttatctg agacaagctt tacaggtgac atcatcaaat
     8521 atgatcagca aggccagcaa attatcgtga aaaatttttc caaaaatgtg agccggatta
     8581 tccgtataag cgatattcaa cgcctgcgat ttgtcccctc aactgtccaa acagcccaaa
     8641 aaaatagatt taagaaagag tgagatgtag ttgcttcatc ccactctttt tcttagcgaa
     8701 tttattcaaa atgtaaatga actgcgatat gatctccata accacttctt tccaagtcac
     8761 gttgtaaacg ataggaaata tagtgttctg caatggtaat gtaacctgcg cccaataaac
     8821 gatgttcaac catagattga atcatactga tagtcgcaca ttccaccttg gcttcttgta
     8881 aatccaaaac taccttctta gtgacttgag caagattttg acgcaaatca tctgtcaaaa
     8941 cataaacagt ttgggctgcc ttcaagatgg cttggtaaat cttatctgga ttaaattcag
     9001 caatttcgcc attacgtttg attacttgca taggtttctc ctttattctt tgttttcttt
     9061 gatttctgcc agcatttttt cttcttctac tgtcagttga taatgttcaa gtaaatctgg
     9121 tctgcgctcg taggttttct ttaaactctc gtacaatcgc cactgacgaa tcttttcatg
     9181 gtggccactc atcaatacat ctggcacgac catgcctcga taatcatagg gacgtgtgta
     9241 ctgaggatat tctaaaagac ccgaagaaaa actatcatct tggtggctag actccttgcc
     9301 aatcacttct ggaatcaggc gaactgtagc atcaatcatg gtcatagctg ccaattctcc
     9361 accagtgagg acatagtcgc ctagggaaat ctcatctgtt accaaggtct taatgcgctc
     9421 atcataaccc tcatagtgcc cacagataaa gattagctct tcctcttgag ccaaatcttc
     9481 agcataagcc tgatcaaact gctttccagc aggatcgagg agaataacgc gcggattttt
     9541 cttttcaata gcatcaaagg aatcgaaaat aggttgtgct ctgagcaaca taccctgacc
     9601 gcctccgtag ggctcatcat ctacatggcg ggccttttca gcattttctc gaaaattatg
     9661 atactggata tccaagagcc ctttttctcg agcctttcca acgattgagt gctccagtgg
     9721 agaaaacatc tctggaaaga gggttaaaat atcaatcttc atcgtctaac ccttctaaga
     9781 tttccacatc gacccgttta tttggaatat caacattgag aaccactggt gggatataag
     9841 gtaaaagcaa atcacgtttg ccttttcgtt tgaccaccca gacatcatta gcacctggtt
     9901 gcaggatttc cttgatggtt ccaaccaagc tatcaccctc atagacttcc aaaccgataa
     9961 tctcgtgata gtaaaattca ccatcgtcta ggtcattcaa atcttcctca gcgaccttga
    10021 gactatatcc cttgtacttt tcgatagtat tgatatggta catatctttg aatttaataa
    10081 tgtcaaagtt cttctgttta cggtggctag cgatggtcac tgtttggaca aactgatctt
    10141 tttcatcaaa caaagccagc tcagctcctt ttttaaaccg ttcttctgca aaatccgtca
    10201 cagacaagac tcgcatctca ccctgtaatc cctgcgtatt aacgattttc ccaacattaa
    10261 agtagttcat cttgtctcct gtaatctcct tttttccatc ttattctgac aattctcaaa
    10321 taatagccgc aattttttcc gattctgacc attgtaaata atggtgattc cctcctaaaa
    10381 tgagtttagt attggaagtc caatattctg attctctgta ctctttttct ctataaggct
    10441 gacaaaaaac aaatacagga atatgagctt ctatagatac atcctcaaaa tcttcctcag
    10501 taatctctcc agatatctga aattctggat cttgattttc caactctaag cctttttctt
    10561 gcattaattc ccagattttt ttattcgttt caggactaaa tgttgcttga gttaagttct
    10621 taaaataaag ttcaggacca cactcgtcaa tcagcctcat ctgctcttcc atttctggat
    10681 aaggattttc tgaaaaatca gcaaacatga cttttttagt tgtcggttca attgctacta
    10741 aagtctgacg cttaattggt ttctcgagta atttgcaagc taaaattcca ctccaactat
    10801 gtgcacaaag tatatattca gaaattccta attcttcaag tacttcataa accgcatctg
    10861 caagattatc tagatttttt ccagcttggt catgaatcgg actcctacct gtgttcggaa
    10921 aatcaattgt caaataacca attgtaggag gaggtttttc aagtataagt gaaaaatttt
    10981 cataacttgg tagcaaacct gctccgttta gacaaactag cactttcttt tgcttttgat
    11041 aagtaacaga gaggctacca atttctgtag atacttcaaa cctcttcata aagaaatcca
    11101 ctcattctat ataatgaatt attaaaaatc cttatccttt attttatcac gttccaagga
    11161 ttttctcaag ttggaggaaa gggacaacat ctctactttc ccttcaagaa tccttccaaa
    11221 ttatgtttat gttggtaatt aacggctgcg gttttgtctt tctcaaagac agtcttggta
    11281 aggtcaatat gattaatagc tacgattgcg acggtgtagt aaatgatatc agccagttct
    11341 ctggcaagtt cctcgttcga atcctatccc ttcttttcga ccagagcgcc tattcaaaac
    11401 ctcggctact tctccgactt cctccactaa cttcataaag agaccttcat cagtccgaga
    11461 ctgctgttaa tgttcgagta agtagtcttg gaattgccta aacgttcaat cttttatagt
    11521 atattgaaac tagaatagta cacctttact tctaaaacat tgttagaaat cgatttgact
    11581 gtcctgatcg atttgtcctg ttattatttc tttttactat atcttctatt ccacacaaaa
    11641 aagcgagaca tccgtcccgc ccttcttatt tttcgtcaat aacgattctt acttttttgt
    11701 attcagttgg gacagagtag acaatcgttc ttatcgcaga aatagtgcga cccttacgac
    11761 cgattacacg acccacatcg ctttgatcaa gattcaaatg atattccaaa aattctggtg
    11821 tatcctcaat cttgatagtt aaggcatctg gttgtgaaat taagggtttc acaatcgcaa
    11881 taatgagatt ttcaatcgta tccatctgtc aacctacttt aaacttattt tgagaattta
    11941 gaatcgtgga attttttcaa tacgccttct tttgaaagga tgttacgtac tgtgtctgaa
    12001 ggttgagctc cattagccaa ccatgcaaga acgcggtctt ctttcaaagt tacttggttt
    12061 tcagcaacaa gtgggttgta agttccaact gtttcgatga aacgtccgtc acgtggtgaa
    12121 cgtgaatctg ctacgttgat acggtagaaa ggttttttct tagaacccat acgagtcaaa
    12181 cggattttaa ctgccatttt taaagtctca tttctttaat tttttatttc ggtgaaatag
    12241 ctgagctatt tagcacatgt tctattatag cagatttctg gcaggtgtca agaaaaaaac
    12301 ttgacagact ataaatttct taaagcattt agaaatttgt tagaatagga aataaatgtt
    12361 tgaaagatgc tatccgtgaa tactatttta gaaaaatttt ataaagaaca ccaagtcaaa
    12421 cccttcatat cgcctgaacg agatttggat acttggcttc taagtcccaa acccgttccc
    12481 aagcgaaata tggacttatt agcagatgat tcactagcag gagatatcat tttactatgg
    12541 agaatccagt ttggtacttt taccactgaa acatgatttt aaaaactccc attcgaatat
    12601 gattcaaaat tcgcttagaa tcaatgtttt cccacttttt caaaaaataa attttcatct
    12661 aatttaaata actttcaatt aagtgtgaat tttgcttaaa atcataaaaa aacaccccat
    12721 ggtgtgaacc atgaagtgtt gtaaatgctg tattgtagct agttcttaac gtttagagaa
    12781 ctggctagct ttacgagctt tcttaagatc tggttcccta aatactttga gtacacttat
    12841 ggcattgatg cccctaaaca tctaaaaact ctggtagaaa aagagtatgc tcttgtcgaa
    12901 acttcttttg actcacttga ccatctaaat actaccatga aaaagaatat cctgaagaag
    12961 aagggagtta ctggactttc taaaatgaaa tcttgcgacc tggatcaagc tcttcatgag
    13021 catttttcag aagaagaatt agctggtcac tttcatgtcc ttctatggac ttttttacaa
    13081 tggcattgct atcacaccca atacctatct aagcgcctgg ttcgtaaact ttattgcagc
    13141 tcttcctcta aatttcctaa ttgttgaacc aattgcccgt tttatactaa gttcttttca
    13201 gaaaccattt actggggaag aagttgaaga agttgaagat tttcaagatg atgatgaaat
    13261 cccaactatt atctaagcca gttctgtaaa ctactaatat ttgaaatcca cttcctttta
    13321 gggtacaatg gtataaatga atttttgaga ggaacagaat gaaaaaacta gcaacccttc
    13381 ttttactgtc tactgtagcc ctagctgggt gtagcagcgt ccaacgcagt ctgcgtggtg
    13441 atgattatgt tgattccagt cttgctgctg aagaaagttc caaagtagct gcccaatctg
    13501 ccaaggagtt aaacgatgct ttaacaaacg aaaacgccaa tttcccacaa ctatctaagg
    13561 aagttgctga agatgaagcc gaagtgattc tccacacaag ccaaggtgat attcgcatta
    13621 aactcttccc taaactcgct cctctagcgg ttgaaaattt cctcactcac gccaaagaag
    13681 gctactataa cggtattacc ttccaccgtg tcatcgatgg ctttatggtc caaactggag
    13741 atccaaaagg ggacggtaca ggtggtcagt ccatctggca tgacaaggat aagactaaag
    13801 acaaaggaac tggtttcaag aacgagatta ctccttattt gtataacatc cgtggtgctc
    13861 ttgctatggc taatactggt caaccaaaca ccaatggcag ccagttcttc atcaaccaaa
    13921 actctacaga tacctcttct aaactcccta caagcaagta tccacagaaa attatcgaag
    13981 cctacaaaga aggtggaaac cctagtctag atggcaaaca cccagtcttt ggtcaagtga
    14041 ttgacggtat ggatgttgtg gataagattg ctaaagccga aaaagatgaa aaagacaagc
    14101 caactactgc tatcacagtc gacagcatcg aagtggtgaa agactacgat tttaaatctt
    14161 aaaaaccaaa aaaatacagt atccacattc ggtactgtat ttcttttact ctcattctta
    14221 agttaaatta ttaaaatccc atatttggtc tatccagcct tcataaaagt ctggctcgtg
    14281 gcagaccata aggatagatc ccctatattc tttgagagcg catttgagct catcctttgc
    14341 atccacatcc aaatggttgg tcggctcgtc cagcactaaa acgttgtttt cacgattcat
    14401 caagagacag aaacgaacct tggcttgctc tccccctgat aatacttgaa tctggctttc
    14461 aatatgtttg gttgtcaaac cacaacgggc aagggctgca cggacttctg cttgattaag
    14521 ggcaggaaag gcattccaga cagcttcaag aggagtttgg cgattaccac cttctacttc
    14581 ctgttcaaaa taaccaagtt ctaaataatc tccacgctcc acttccccag cgattggcga
    14641 gataatgccc aagagactct tcaagagagt tgtttttcca ataccattag caccaataat
    14701 cgcaaccttt tgattgcgtt cgaaggtaag atttaaaggc ttagtaagag gacggtcgta
    14761 accaatttgc aagttcttgg cttggaagat aaagcgccct ggtgtacgag ctggtttgaa
    14821 atcaaaggat ggttttggtt tctcactttg gagttcgata atatccatct tatccaattt
    14881 cttttgacga gacatagcca tattacgagt tgcaacacgg gctttattac gagccacaaa
    14941 gtccttgagg tctgcaatct ctttctgatg gcgttcgtag gctgcctcta gctgagattt
    15001 cttcatagca taaacttctt ggaactggta gtagtcacca gagtaacgcg tcagctgttg
    15061 attttccaca tgatagacaa tattgataac gtcattgagg aatggaatat cgtgcgaaat
    15121 gagaacaaag gcattctcat agttttggag atagcgcttg agccaatcaa tatgctcagc
    15181 atccaagtag ttggtcggct cgtccaacag caaaatatca ggcttttcaa ggagaagttt
    15241 tgccaaaagc accttggttc tttgcccacc tgacaaagaa gttacatccg tatccatgcc
    15301 aaagtccata acaccaagag cacgcgctac ttcgtcaatc ttagcatcca aggtatagaa
    15361 atcacgactc tccagacggt cttgaagttc tcctacttct tccatgagag catcaacatc
    15421 cgcgccgtct tcagccattt tcatatagag gtcattgata cgagcttcag ctttgaaaag
    15481 ctcatcaaaa gccgtacgga gaacatcacg caccgactgt ctttcagcaa ggacagagtg
    15541 ctgatccaag taaccagccg tcacatattt ggaccactca acctttcctt catctggcag
    15601 cattttacca gtcacgatac tcataaaggt tgattttcct tcaccattgg caccgaccag
    15661 gccgatatgt tctcccttga ggagacggaa ggacacatct tcaaaaattg cacggtcacc
    15721 aaaaccgtga ctcagatttt taacttctaa aatactcatt ttaattcctt atcttgtttt
    15781 tatgtaatcg tttataaagg agccaagcca gatagccacc caaagtgttg gtccacaaat
    15841 catcaatctc aaagacacga ttgaaatcaa agaaaaagtc caagattaat tgcgtacact
    15901 cgattccaag actcacaaga aaactaaaaa gaaggacctt ttttgttttc cgcaaatttg
    15961 gaaatagata aaggagttgg aaaatcagag gaaaaaacaa gaagacattg aggatatttt
    16021 gtaaaaaaat ccaacataat tgtccaatgt cactcacttc gcccagtttc cagagagaat
    16081 tgaaaggagt caaaagaaaa accaggcgtc caagatgctg aatatctgga gttcccactc
    16141 ccacggtaga ttgttcttga ggagtaaagc aaaaacagac aatgcaaatg ctatagaaaa
    16201 tgactcccca gaccaaaata tgattataag tcttcttcat cattaaggat ttaccgctgc
    16261 gactgccttc tggcggtcac gtttcattgt gttagagcgc aattgtccac aagctgcgtc
    16321 aatatctgta ccatgctctt gacgaaccac acagttgacc ccttttttct taagcgtatc
    16381 atagaaagcc aacacgcgct ctttgggact acggctatat tggtcatgct cactaactgg
    16441 gttataagga atcaagttta cataagacaa tttcttgatg ttcttgagca attcagccaa
    16501 ttccaaggct tgttctacac cgtcgttgac ttcattaagc atgatatatt caaaggttac
    16561 acgacggttt gttgtctcaa tgtagtattc aatagcagca aagagttttt caatcggaaa
    16621 ggcacggtta attttcatga tacttgaacg aagttcattg ttgggtgcgt gaagagacac
    16681 ggcaagattg acctgaaccc cttcatcagc aaagtcacga attttatgag ccaaacctga
    16741 ggttgaaacc atgatgtgac gagcaccgat agccattcct ttatcatcat tgatagtacg
    16801 aaagaaattc aaaacattgt tgtaattatc aaagggctca ccaattccca tgacaacgat
    16861 atggctgacg cgttcatcct gaccacgctc atcaaagtat ttctgaacca gcatgatttg
    16921 cgctacgatt tcaccgttat tgaggtcacg ctgcttctta atcaaaccag aggcacagaa
    16981 ggtacaaccg atattacagc cgacctgagt ggtcacacag acagataaac catagtgttg
    17041 acgcatgagt acagtctcaa ttaacatacc gtcgggcaat tcaaagagat atttgactgt
    17101 accatcagca gactcttgca caatacgttg tttcaaggga ttgaccacaa actggtcatt
    17161 gagcttagca atcaaatcct tggaaaggtt ggtcatttct tcaaatgact gcacacgttt
    17221 acggtagagc cattcccaga tttgatctgt acggaatttc ttttctccct gctccaatac
    17281 ccattcctgc atggtttgat gtgccaaact atgaattgag ggtttcattt cttctcctta
    17341 ttctctactc acttctgacg aatgacaaaa tgacgttgtc ccttgtcctc tttctgagaa
    17401 tgctggtctt tctgacgacg tctatttttc ttatctgcat tcgactttcg tttagtttga
    17461 gtcggtttct ttccttttct agaaggtgtt tcttcttccg tcttacgcat tttcttgtca
    17521 aatgatgctc gcttaggggc ttcattttct aagacaaaat aggcacaacc ataactacaa
    17581 tactctaaaa ggtagtcttg taaacgactg attttttcaa gtttttcttc tgttcggtca
    17641 tccttgtaaa aacctcgtag gcgaagctgt tcgttgctcc agtcccccac gatataatca
    17701 aacttggtta agacttctga aaaacgctga ttaaaagtcg tcacatcaaa ggcatccttg
    17761 atattttcaa ccaaggaaaa agctatccct tccgtttcga ccttgtcccc gtgtaaatgg
    17821 aactccggac caggaaactt gttatagttg tataattcag gtgcaatttc ttttcgcata
    17881 gatatccttt ttccacgatt acttaatact ttattctacc ataatttcta gcagttagca
    17941 cgtttctcat aaaaatgaaa aaagtctgac gattttatca gaccagaatc ttataaccta
    18001 aaaagagaag aacaattctt ccctccaact atcattattt agcagctgcg tacaattcat
    18061 ctactttatt ccagttgatt actgaaaaga aagctttgat gtagtcagga cgcacgttgc
    18121 ggtatttcac gtagtaagca tgttcccaaa cgtccaagcc caagattggt tttttacctt
    18181 ctgagattgg tgtgtcttgg tttgctgttg aagtcacttc aagtttccct tctttgttga
    18241 caaccaacca tgcccaacct gaaccaaaac gagttgttgc tgctgcagtg aaggctgctt
    18301 ggaattcttc aaatgaacca aatgttgcat cgattgctgc tgccagttct gctgaaggag
    18361 ctgttttctc gggagtcatc aattcccaga aaagagcgtg gttcaagtgt ccgccaccat
    18421 tgttgataag tgcttgacgg atatcagctg ggatagattc tacatcagca agcaaggctt
    18481 caaggtcttc accgatttca gggtgttttt ctaaagctgc attggcattg ttgacataag
    18541 tttgatggtg tttgtcatgg tgcaaatgca ttgtttccgc atcgatgtat ggttccaaag
    18601 cgtcgtatgc atatggaagt tctggtaaga taatagccat ctgtaatacc tctttttctt
    18661 tctatatgaa aatgataacg caaacattca cttttttcaa gttttttgct tatagataag
    18721 gaaatcactg acatactttc taataaaata agcaaatcag caagaaaccc acgacaagat
    18781 aatcctgacg cgggttgtag tttcaacaaa tgtcaattga cctgactagc aatctgtaag
    18841 agtgcctttt caaaaaggaa acctttttca taaagacctg tcttaatctg atagtctgtc
    18901 tcaatcaaat aggaaatagc ttgcttcaga aagctcaaag aaagtcctct cgaatctctt
    18961 aatgcaaact tgatttgata aggatttggg ttacgtccca gataactacc taaactactt
    19021 gcaatctgcg attctgtttg gccagactcc gccaaaatct tcacctgagt aaaagtccga
    19081 aattgtccca gcatgactgc aatcagtttg atttcatctt ccccttgcaa ggtcaagtct
    19141 ctcaccaaat cgcgcgcctg atccatcttt ttagtcagaa taaactgagt taaatcaaaa
    19201 atattgtcct gcaaggtctt gggaattgcg ttaacaatat cctcttcctc gataacagaa
    19261 tccgccttat aggactgtaa aaagagaaga tttttctgga tttcgctaaa ttgaaacccc
    19321 gacttgatga ggagattttc aaaagaatga ttggtaaact gcagaccttg tttctgactc
    19381 cacttttgga agtactggcg caattcttgt tcttttactt ctactgcatc gaaggccttg
    19441 gcatcacgct taagtaattt gactaaccgt cttttgctat ccagctttcc ttctgcaaag
    19501 attatcaact tggttgttgg agaaggattg tcaaggtatt cctcaaatga cttaagctca
    19561 tcatctgtca aaaagcgttt cttagcagtc gtgatatcca taaaataatc taatatcacg
    19621 attttctcat ccgcaaagaa aggaaggctg accaactcca gttccacatc cttgtaaact
    19681 acttctttca tatcaaagta ggcaaagttg aggtcagcag aatcataccc aatctgtttc
    19741 aacacttgac tcttcatcac ttcaaactga ccctgatctg tccctgtaaa taggctcagg
    19801 ctcggtaaat ttgataaagt caacttctga ctttcttcaa tggctagcat cgtctctcct
    19861 ttcttcagat ttttcgattt aatttagtca atatagcgca atttcccacg gaaatcttct
    19921 aagctctcgt agcctttttc caccatgatt gctttcagtt cattggtaat gcggtcaaaa
    19981 gcactgacgc cttctttgtg aagggtcgtt cccacctgca ccatacttgc tccacagagg
    20041 atgtgttcaa aggcatctcg accagtcaga acgccacctg ttccgataat ttggatttga
    20101 ggatttaaac gttgataaaa ggcgtgaaca ttggctagag cagtcggttt gatgtattct
    20161 ccaccaattc caccaaaacc attcttaggc cgaataacga cagattcgtc ttctatatag
    20221 aggccgtttc cgatagagtt aacgcagttg acaaacttga gcggatattt gttgaaaata
    20281 gctgccgctt gatcaaagtg aacaatatca aaataaggtg gcaatttaat tccaagaggt
    20341 ttggtgaagt aagcaaacac ttctgccaaa atccggtctg ttgtctcaaa atcataggca
    20401 atctgaggtt tacctggaac atttggacag gaaagattta gctcagtcag accacgaaaa
    20461 tcactctctt ggactttttt caaaatagta tgggtttcct ctggagacat gccgaccaga
    20521 gataagaaga aagttcggtt cgactctttt tcctgcaaat ctaaaagata atccaaataa
    20581 tagtctaagc cattatttgg caagcccata gagttgatgg aaccaagtgg aacatcttgg
    20641 tagcgtggct caggattccc ctgacggaag tccaaggtcg ctgtcttagt aacaaaggtt
    20701 cctgccgctg agtttttgac ctcttctaac tcctctatcg tcatacaagc cacacctgct
    20761 gcattcatca agcaattgtc aaactcaaaa ccagcaattt gtgttttcgt tgataccatg
    20821 attctaatcc tccagatttc ttttattgct aatattatac cataatttta attctaggtt
    20881 tacta
//
LOCUS       DAVKDZ010000030        20865 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000030 DAVKDZ010000000
VERSION     DAVKDZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20865
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.031"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(85..1401)
                     /locus_tag="VWZ30_001118"
     CDS             complement(85..1401)
                     /locus_tag="VWZ30_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU9334874.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVNIFDAEAHFLS
                     NKVIEIQAGDEKQELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKL
                     GVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYLEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            1595..2821
                     /locus_tag="VWZ30_001119"
     CDS             1595..2821
                     /locus_tag="VWZ30_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU9334875.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            3020..4594
                     /locus_tag="VWZ30_001120"
     CDS             3020..4594
                     /locus_tag="VWZ30_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9334876.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQNIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVSPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFVDE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYCKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            4858..5646
                     /gene="codY"
                     /locus_tag="VWZ30_001121"
     CDS             4858..5646
                     /gene="codY"
                     /locus_tag="VWZ30_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEU9334877.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDNYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGQLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            5646..6221
                     /locus_tag="VWZ30_001122"
     CDS             5646..6221
                     /locus_tag="VWZ30_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9334878.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTGGRNLYGDLGIFYQEHDSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFSLGHFKNTLGAKLVDENLNELSE"
     gene            6303..6388
                     /locus_tag="VWZ30_001123"
     tRNA            6303..6388
                     /locus_tag="VWZ30_001123"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6337..6339,aa:Leu,seq:aag)
     gene            6671..7801
                     /gene="ugpC"
                     /locus_tag="VWZ30_001124"
     CDS             6671..7801
                     /gene="ugpC"
                     /locus_tag="VWZ30_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU9334879.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(7923..8093)
                     /locus_tag="VWZ30_001125"
     CDS             complement(7923..8093)
                     /locus_tag="VWZ30_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334880.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEVSNHTYGKATLTWFEEI
                     FEEYKEHHNIDA"
     gene            8301..9059
                     /locus_tag="VWZ30_001126"
     CDS             8301..9059
                     /locus_tag="VWZ30_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9334881.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMKLSGMIYDEGWLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            9146..9658
                     /locus_tag="VWZ30_001127"
     CDS             9146..9658
                     /locus_tag="VWZ30_001127"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU9334882.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGIVAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            9841..10785
                     /locus_tag="VWZ30_001128"
     CDS             9841..10785
                     /locus_tag="VWZ30_001128"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU9334883.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDEDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYRYL"
     gene            10794..11471
                     /locus_tag="VWZ30_001129"
     CDS             10794..11471
                     /locus_tag="VWZ30_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEU9334884.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            11569..12327
                     /locus_tag="VWZ30_001130"
     CDS             11569..12327
                     /locus_tag="VWZ30_001130"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU9334885.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLSAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            12365..13870
                     /gene="lytC"
                     /locus_tag="VWZ30_001131"
     CDS             12365..13870
                     /gene="lytC"
                     /locus_tag="VWZ30_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEU9334886.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKADGSKAQSEWIFDNYYKAWF
                     YIKSDGRYAQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKVIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGTEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDANQTIELLKKYKMNLSYP
                     IYYDVENWEYGNKTKRAPSDTGTWVKIINKYMATMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPHYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            14476..14994
                     /locus_tag="VWZ30_001132"
     CDS             14476..14994
                     /locus_tag="VWZ30_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU9334887.1"
                     /translation="MIELKKEAAKDVATLTKAAPVALAKTKEVLNQAVADLYVAHIAL
                     HQVHWYMRGRGFMVWHPKMDEYMDALDGHLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEVGEFRNVEESLERVLEIYRYLITLFQEALDVTDKEGDDVTNDIFVGAKADLEKTVW
                     MLAAELGQAPGL"
     gene            15124..15630
                     /locus_tag="VWZ30_001133"
     CDS             15124..15630
                     /locus_tag="VWZ30_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU9334888.1"
                     /translation="MTKKIVAIWAQDEEGVIGKDNRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNSEEKTDGVATFHDIQSVLDWYQAQDKNLYILGG
                     KQIFQAFEPYLDEVIVTQIHARVEGDTYFPEEFNLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            15630..15800
                     /locus_tag="VWZ30_001134"
     CDS             15630..15800
                     /locus_tag="VWZ30_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334889.1"
                     /translation="MERSLFGLFTAFLCFICLLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            15832..17064
                     /gene="clpX"
                     /locus_tag="VWZ30_001135"
     CDS             15832..17064
                     /gene="clpX"
                     /locus_tag="VWZ30_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU9334890.1"
                     /translation="MPTTRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKEAVDGTDKPILETA"
     gene            17073..17660
                     /locus_tag="VWZ30_001136"
     CDS             17073..17660
                     /locus_tag="VWZ30_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEU9334891.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRNNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGRMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDQAWDAILEKL"
     gene            17671..18051
                     /locus_tag="VWZ30_001137"
     CDS             17671..18051
                     /locus_tag="VWZ30_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU9334892.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ETIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            18103..18993
                     /gene="rapZ"
                     /locus_tag="VWZ30_001138"
     CDS             18103..18993
                     /gene="rapZ"
                     /locus_tag="VWZ30_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU9334893.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            18990..19967
                     /locus_tag="VWZ30_001139"
     CDS             18990..19967
                     /locus_tag="VWZ30_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU9334894.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVVGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            19964..>20865
                     /gene="whiA"
                     /locus_tag="VWZ30_001140"
     CDS             19964..>20865
                     /gene="whiA"
                     /locus_tag="VWZ30_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU9334895.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAVLSDEEAGRTYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIA"
BASE COUNT         6282 a         3781 c         4696 g         6106 t
ORIGIN      
        1 acaaaagagg gaaaagacag atttggtgtt tcgtagaagt aaaaacagcc caaagagggc
       61 tgtcagttaa gatgagattt caacttaaat cgcaaacaag tcattcaagt tctcagccaa
      121 ggttgggtga gtgaagattt gttttgtgaa gtaagtgtaa ggaatcttgt tgtccatagc
      181 aacagtaatg atgttgatga tttcttgaga accttctgag aagatgcttg caccaagaat
      241 ttcttttgtt tcagtattaa caacagcttt gaaagctccg cgaaggtctc catttacgtg
      301 accacgaggc atggctgcaa cagggatttc tttcactgcg tatggaagtt tcaaatcagc
      361 tgcttggctt tcagtcaaac caacttgtga aagtgcaggt gtgatgaaca tagtatttgg
      421 cacattgaga cggtcctcaa gtgtgtagct gccatctcca gcaaggtagc tataaacaac
      481 acggaagtca tcaagtgaaa tgtaagtaaa ttgaagacca ccgttgacat ctccaactgc
      541 aaagacacca ggaacgtttg tttgacagtg tttatctact ttaatagcgc cacgttcagt
      601 tagttcaata tctgtatttt caagttgaag tggttctaca tttggtttgc gtccagttgc
      661 gtagagaagg gcgtcgaaac ggtaagtttc gtcttcagtt acgacaagca cttggtcacc
      721 gtcgttttta atttcagtag tatggatatt ttgaagcaat tcaataccgt cttcttccag
      781 gtattgttta gcaagagctg cgatggaagg ttctgcacga ggtaggaatg tatccaaggc
      841 atctaggact gtaaccttgc ttcctagttt attgtaaagg ccagcaaatt caagaccgat
      901 atttccgcca ccaaggactc caagtttttc aggcaattta tccaagcttt ggatacctgt
      961 tgagtcaaag acgtttttgc ttgtagcaag tccagggatt ggcaagacgt ttgaaacagc
     1021 accagtgttg atgacgatag tttcagcagt caattcttgt ttttcatcac cagcttggat
     1081 ttcgatgact ttatttgaaa ggaagtgagc ttcagcatca aagatattga cgccagtacc
     1141 agcaacagtc gtatagtttt taccgttgag gcgaccagtg atcgtgtttt tggtagcaat
     1201 gacttcttca aaagacaagt ctttctcagc agcaaccagc aaagttttag ttgggataca
     1261 accgatgttg atacaagttc caccgtacat agctttgcta cgttcaacga gggcaacttt
     1321 tttgccagct gaagccaatt taccagctag tgttttacca gctttaccaa atccgataac
     1381 gattaaatca taagttaaca tttagaattc ctcctattcg aattacattc tagcacaaga
     1441 aaaaaacttt tgcacaattc tgctacgaaa aagacagaaa aagataaaaa cgctttccag
     1501 acaaaaaact agtaattttt taacaagctt gataaactaa aatcctatct atttttatat
     1561 aagaaagaat aaatgttcga aaataggagg ttttatgaaa tcgaatcgtt atattattgc
     1621 ctttgctggg gtcattttac acttaatgct gggttcgact tatgcctgga gtgtttatcg
     1681 taaccctatt attgaaaaaa cgggatggga tcaggcttct gttgcctttg cctttagtct
     1741 agcaatcttt tgtttgggct tatcggctgc atttatgggg cgtttggtag aaaaatttgg
     1801 tccaaaagtc atgggaagtc tatctgcttt tctatacgca ggtggaaata tcttaacagg
     1861 atttgcaata gaccgtcagg agctgtggtt gttgtatctc gcttatggca ttttaggtgg
     1921 gcttggtttg ggagcaggct atattacccc tgtgtcgacg attataaaat ggtttcctga
     1981 taaacgtggt ctcgcaacag gtttagcgat tatggggttt ggttttgctt ctttattgac
     2041 tagtcccata gcgcaacacc tcatcgcagg ggtagggctt gtagaaactt tttatatttt
     2101 aggagcaagt tactttatta tcatgctcct agcttcacaa ttcattaagc gtccaaatga
     2161 gcaagagctt gcaattttat cttcttcagg gaaagaaaaa acagcctctt tgacgcaagg
     2221 aatggctgca aatcaggctc taaaaagcaa tcggttttat atgctttgga ttattttctt
     2281 tatcaacata gcttgtggtt taggcttaat ttcagcggca tcgccaatgg cacaggagat
     2341 ggctggcttg tctacaagtc atgcagcagt aatggtgggt gttttgggga ttttcaatgg
     2401 atttggtcgc ttgctctggg cgagtttgtc tgattatatc ggtcgccctc taacctttag
     2461 tatattactg cttgtcaatc ttttcttttc tctctcactt tggctcttta cagattccgt
     2521 tttatttgta gttgctatgt ctattttgat gacttgctat ggagctggtt tttctttgat
     2581 tccagcttat ctcagtgata tttttggaac caaggaattg gccgctctac atggttatat
     2641 tttaacagct tgggcaatgg ctggtttagc gggacctatt ttattagcag agacttataa
     2701 aatggctcat tcgtacacac aaaccttgtt cgtttttctc attttataca gtattgcctt
     2761 ggctttgtct tattatctag gtcgttcaat caaaaaagaa agtcaaaaac cgcttacatg
     2821 attttctgta gaaatggggc tatcttttgc tctggtcttg tgttatacta gataggttgc
     2881 aaagaaaaca gtacttttct tttgtggaaa agaagcaaca cgattttata tctagttata
     2941 tgaaaatacg tcataaaaag aaaagtataa actaagccct atagggtggc ttcgcaccac
     3001 ctttagaaag aagaataacg tgaaatttaa tgaattaaac ttgtctgctg atttgctggc
     3061 agaaattgaa aaagctggtt ttgtagaagc tagtcctatc caagaacaaa ctattccttt
     3121 ggcccttgaa ggcaaggatg ttatcggtca agctcagact ggtacaggaa aaactgcagc
     3181 ctttggcttg cctacccttg aaaaaatccg tacagaagaa gcgactatcc aagccttggt
     3241 catcgctcca actcgtgaac tagctgtcca aagtcaagaa gaactcttcc gctttggtcg
     3301 tagtaaggga gtcaaagtcc gttcagtata tggcggatca agcattgaaa aacaaattaa
     3361 ggctcttaaa tctggtgccc atatcgtggt gggaactcca ggtcgcctct tggatttgat
     3421 taaacgcaag gccttgaaat tacaaaacat tgaaaccctt atccttgacg aagcggatga
     3481 aatgcttaac atgggcttcc ttgaagacat cgaagccatt atttcccgtg tacctgagaa
     3541 ccgtcaaact ttgcttttct cagcaactat gccagatgcc atcaaacgta tcggtgttca
     3601 gtttatgaaa gcccctgaac atgtcaagat tgcggctaag gaattgacaa cagaattggt
     3661 tgaccagtac tatatccgtg ttaaggaaca agaaaaattt gacaccatga ctcgtctcat
     3721 ggatgtggca caaccagaac tcgctattgt atttggtcgt accaaacgcc gtgtggatga
     3781 attgactcgt ggtttgaaaa ttcgtggctt ccgtgcagaa ggaattcatg gcgacctaga
     3841 ccaaaacaaa cgtcttcgtg tccttcgtga ctttaaaaat ggcaatcttg atgttttggt
     3901 tgcgacagac gttgcagcgc gtggtttgga tatttcaggt gtgacccatg tctacaacta
     3961 cgatattcca caagatcctg agagttatgt tcaccgtatc ggtcgtacag gtcgtgctgg
     4021 taagtcaggt caatctatta cttttgtttc tccaaacgaa atgggttacc ttcaaatcat
     4081 tgaaaacttg actaagaaac gcatgaaagg tctcaaacct gcaagtgtag aagaatcctt
     4141 ccaatcaaaa aaacaggtag ctctcaagaa aatcgaacgt gattttgtag atgaaaccat
     4201 tcgtgccaac tttgagaaat ttggtaagga tgctcgcaaa ttggctgctg agtttactcc
     4261 agaagaattg gcaatgtata tcttgagtct gacagtccaa gatccagata gccttccaga
     4321 agtggagatt gcacgtgaaa aaccactacc gtttaaacca tcaggtaatg gtttcggtgg
     4381 taaagctaag ggaggtcgtg gaggccgtcg tggggacgac cgtcgagagc gtgatcgccg
     4441 tggcaatggt cgccgtgatg agttcaaaaa aggaagtcgt ggcaacgatc gttttgataa
     4501 ggaaaaacgt tactgtaagg ataataaaaa accacgcaat actttaagcg aaaagcaaac
     4561 aggctttgtt attcgtaaca agggcgataa ataagaaaaa gcggttcaaa atgaatcgct
     4621 tttttatata aggagaccga atgtgaaaaa gacaaataaa tttattatat tatattgtat
     4681 aatattatga tatgtaaaat aggcaacttg tcaatagaaa aggaacaatt taattaaaca
     4741 tattctggtt tttaattaaa ttgtaagtca atgcaagtca ataaggaatt ttcagataat
     4801 tattgaaaaa gagacgtttt tatgttataa tgatagcgat taaattcgag gtgaaaaatg
     4861 gcacatttat tagaaaaaac tagaaaaatt acatcaattt tgaaacgctc agaggagcag
     4921 ttgcaggatg agcttcctta caacgctatt acgcgtcagt tagcggatat tattcattgc
     4981 aatgcctgca ttatcaatag taagggacgt ctgcttggct attttatgcg ttataaaaca
     5041 aatacagatc gcgtagagca attcttccaa actaagattt tcccagataa ctacgttcaa
     5101 ggggctaata tgatttacga aacagaagca aacttgcctg ttgagcatga tatgagtatt
     5161 ttccctgttg agagtagaga tgattttcca gatggcttga cgactattgc accgattcat
     5221 gtatcgggga ttcgccttgg ttctttgatt atttggcgta atgataaaaa attcgaagat
     5281 gaggacttgg ttcttgttga gattgccagt accgttgttg ggattcagct tcttaacttc
     5341 caacgtgaag aagatgagaa aaatattcgt cgtcgtactg ctgtcaccat ggcggtcaat
     5401 accctttctt actccgaact ccgtgctgtt tcagcaattt taggggaatt aaatggaaat
     5461 gaaggacagt tgactgcgtc agtgattgca gatcgtatcg gaattactcg ctctgtgatt
     5521 gtcaatgctc ttcgtaaact tgagtctgcg gggattattg aaagtcgctc acttggaatg
     5581 aagggaacct atcttaaggt cttgatttca gatatttttg aagaagtgaa gaaaagagat
     5641 tactaatgac aaaggcttta atttcgattg attatacaga agattttgtt gctgatagtg
     5701 ggaagctgac agcaggtgct ccagctcagg cgatttcgga tgccatcagc aaggtaactc
     5761 gattagcttt tgaacgagga gattatatct tctttaccat tgatgctcat gaagaaaacg
     5821 attgtttcca tccagaaagt aaactatttc ctcctcataa tttgattggg actggtggac
     5881 gaaatttata cggagatttg gggatctttt atcaagaaca tgattcagac agtcgtgtct
     5941 tttggatgga taaacgccat tactcagctt tttcagggac tgacctagat attcgtttga
     6001 gagagcgtag agtgtctacc gttatcttaa caggtgtctt gacggatatc tgtgtcctac
     6061 atacagctat agatgcctat aatctaggat atgacatcga gattgttaaa ccagctgttg
     6121 cttccatttg gcctgaaaat catcaatttt ccctaggtca tttcaaaaat acactcggag
     6181 ctaagttagt agatgaaaat ctaaatgaac tttctgagta atctggttgg cgaaaatgaa
     6241 aaaatttgaa aaaaagtgtt gacaagcatc ccaaaagttg atatactagt aaagtaatcg
     6301 acgcggggat ggcggaattg gcagacgcgc aggactaagg atcctgtgac cgctttaggt
     6361 cgtgagggtt caagtccctc tctccgcata ggaaaagagt tagctaaagc tagctctttt
     6421 gtatttctct aaaaaattag catccagtca aatgtagtgg agaatcatcg aaaatatgag
     6481 aaaactgatc cttgtttctg cttttgtcat agaagaataa tcaagattat cttcttcaaa
     6541 ttctccgata ttctctaaag ttttgtgcaa gttgcacaga acttgtttat ttttttggtc
     6601 atcttgccat agaaatataa agcgttttca tatataatat aattatcaaa agacaaaagg
     6661 agttcacctc atggtagaat tgaatcttaa aaatatttac aaaaaatatc caaacagcga
     6721 acactactca gttgaagatt tcaacttgaa catcaaagat aaagaattta tcgttttcgt
     6781 aggaccttca ggatgtggta aatcaactac actccgtatg attgctggtc ttgaagacat
     6841 tacagaaggt actgcatcta tcgatggcgt ggttgtcaac gacgtagctc caaaagaccg
     6901 tgatatcgcc atggtattcc aaaactacgc tctttaccca cacatgactg tttatgacaa
     6961 catggctttc ggtttgaaat tgcgtaaata cagcaaagaa gacattaaca aacgtgttca
     7021 agaagcagct gaaatacttg gattgaaaga attcttggaa cgtaaaccag ctgacctttc
     7081 aggtggtcaa cgtcaacgtg ttgccatggg gcgtgcgatt gtccgtgatg cgaaagtatt
     7141 cttgatggac gaacctttgt caaacttgga tgccaaactt cgtgtatcaa tgcgtgctga
     7201 aatcgctaaa attcaccgtc gtatcggagc tacaactatc tatgtaactc acgaccaaac
     7261 agaagcgatg acacttgcag accgtatcgt tattatgtca gctactaaga accctgctgg
     7321 tacaggtact atcggacgtg tagaacaaat cggtactcct caagaagttt acaaaaatcc
     7381 agttaacaaa tttgttgcag gattcatcgg aagcccagct atgaatttca tcaacgtgaa
     7441 attggttggt agcgaaattg tttctgacgg tttccgtttg aaagtgccag aaggagcatt
     7501 gaaagttctt cgtgaaaaag gctacgaagg aaaagaattg atctttggta tccgtccaga
     7561 agacgtgaat gcagaacctg ctttccttga aacattccca gactgtgttg taaaagcgac
     7621 tatctctgta tcagaactgc ttggttcaga atctcacctt tactgccaag ttggtaaaga
     7681 cgagtttgtt gcaaaagttg atgctcgtga ctacttgcaa acaggtgcaa cagttgagct
     7741 tggatttgac ttgaacaaag cacacttctt cgatgtagaa actgaaaaaa caatctacta
     7801 aaataaataa aattcaaagc actacaagaa aagatatctc tttatcaatt gtagtggaga
     7861 gatatcagtt aatctaggga gagaaacaaa atgcttctct tctttttgct agggaagtca
     7921 tattatgcat ctatattgtg atgctcttta tactcttcga aaatctcttc aaaccacgtc
     7981 aacgtcgcct tgccgtacgt atgattactg acttcgtcag ttttatctgc aacctcaaag
     8041 atgtactttg agcagcttac ggctagtttc ctagtttgct ctttgatttc cattgagtat
     8101 tatttgtggg taccatctac aagtgaagct atatgcgtaa actacgtgag caattgaatc
     8161 cgaactagag aggtaataat aaatttatgc tatagttatg gtgacttgta tgcttttgat
     8221 tctagtttat caaataatag attagaattg tcagataata tcattttgtg ttataatgaa
     8281 gaaaaaacag aggtgttcaa atgtcagaag caggtcataa gtttttagca aaattgggga
     8341 aaaaacgctt acgtccaggt ggaaagcgtg ccacagattg gttaattgca gaaggaggat
     8401 tttcaaaaga aaagagaata ctagaggttg cgtgtaatag gggaactaca gcaattgagt
     8461 tggcacagcg ttttggttgc aagataactg ctgttgatat ggatgctcaa gctttagaag
     8521 tggctaaaaa atctgctgga acggcaggtg ttgctcattt aatcagtttt gaaagagcaa
     8581 atgcaatgaa acttccttat caagatgcta gttttgatat tgttataaat gaagctatgc
     8641 tgactatgca agccgatcaa gctaagaaaa aatgtgtaat ggaatatcta agggtattaa
     8701 aacctggagg tcttctcttg acacatgatg tgcttcttaa ggaagctaaa gagtctatca
     8761 gacaggaatt atcacaagca attcatgtaa atgtaggtcc tttaactcaa gatggctggg
     8821 aacaggtgat gatagaatca ggttattgtg atgtgaaagt attgactggt gaaatgacat
     8881 taatgaaatt atcgggtatg atttatgacg aaggttggct aggaactttg aaaatttgtg
     8941 taaatgcttg taaaaaggag aatagaaagc agtttttaac tatgtataaa atgtttgcta
     9001 agaataaaca gaaattgggc tttattgcga tggctagtta taaatcgtca aaacgttaga
     9061 taattattga agttaacttt tccttttttc tttcttaaaa aatatgctat aatagagagt
     9121 aaaaaacttt gaaagaaaga aaaagatgaa tttaaaagat tacattgcaa caattgaaaa
     9181 ttatccaaag gaaggcatta ccttccgtga tattagtcct ttgatggctg atggaaatgc
     9241 ttatagctac gctgttcgtg aaatcgttca gtatgctact gacaagaaag tcgacatgat
     9301 cgtgggacct gaagctcgtg gatttatcgt gggctgccca gttgcctttg agttgggaat
     9361 tggttttgcg cctgttcgta agccaggtaa attgccacgc gaagttattt ctgctgacta
     9421 tgaaaaagag tacggtgtcg ataccttgac tatgcacgcg gatgccatta agccaggtca
     9481 acgtgttctg atcgtagatg acctcttggc aacaggtgga actgttaagg caaccatcga
     9541 gatgattgaa aaacttggtg gtattgtagc gggttgtgcc ttccttgttg aattggatga
     9601 attgaacgga cgtgaaaaaa tcggtgacta cgattacaag gttcttatgc attattaatg
     9661 aaaaacagtc cctagggctg ttttctctac attaggatat aaaaatagac tatagctagt
     9721 tagagaaaaa ctataattga aaactatatc ttcttgcagt ataataaaag gactaagtgt
     9781 ttaagatttg gcttcaaaca tatgcaatta ttcctgaaag agtacagtta ggagagggtt
     9841 atgccgattc gaattgataa aaaattgcca gctgttgaga ttttacggac agagaatatc
     9901 tttgtcatgg atgatcaacg tgctgcccac caagatatcc gtcctttgaa gattttaatt
     9961 ttaaatctca tgccacagaa aatggtcaca gagacccagt tgctgcgcca cttggctaat
    10021 acacccctac aactggatat tgattttctc tatatggaga gccaccgttc taaaacaact
    10081 cgttcagagc acatggagac cttctataaa acttttcctg aagtcaagga tgagtatttt
    10141 gatgggatga tcatcacggg tgctccagtt gagcatttac catttgagga agtggactat
    10201 tgggaggaat ttagacagat gcttgagtgg tctaagactc atgtctattc gacccttcat
    10261 atctgttggg gggctcaggc tgggctttat ctgcgctatg gtgtagaaaa ataccagatg
    10321 gacagtaagc tatcaggtat ttatcctcag gacaccctaa aagagggtca ccttctattt
    10381 agaggctttg atgatagcta tgtatcccct cattcacggc acacggagat ttctaaggaa
    10441 gaggtcttaa acaagaccaa tctcgagatt ttatcagaag gacctcaggt tggggtttct
    10501 attttggcca gtcgtgattt acgagaaatt tatagttttg gtcatttgga gtatgaccgt
    10561 gataccttgg caaaagagta ttttcgagat cgtgatgcag gtttcgaccc acatattcca
    10621 gaaaattact ttaaagatga ggatgttaat caggtacctt gtctttgttg gtcttcatct
    10681 gcagccctct ttttcagtaa ttgggtaaac catgcggtct atcaggagac gccttttgac
    10741 tggagaaaga tagaagatga tgcatctgca tataggtatt tataagagga attatgacat
    10801 atttagacgc ttttaaatca ggtaccttgg ttttaccgag tgccctgctc ttgcatttta
    10861 aggaactctt tccttctagc gacgattttc tggtttggca atttttctat ttgcaaaata
    10921 cgacaggctt agaagaaatg tcgccaagcc agattgctga aaggattggc aaggaaattt
    10981 cggatgtcaa ccagtccatt tctaatctga cggaaagggg actgctccag tatcgtacta
    11041 tcgaattaaa tggcgaaatt gaattgctct ttgatgctag tttggccttg gaacgtttgg
    11101 atgacctgtt tggagcagtt cgttcaagtt cagaccagct aacacctcaa aaccagctca
    11161 aggatttggt ggaaaccttc cagcaggagt tgggacgatt gttgacgcct tttgagattg
    11221 aggatttgac caagacacta aaggaagatg gaaccagtgc tgacttgatt aaggaggctc
    11281 ttcgtgaagc tgttttgaat ggaaaaccaa actggaagta cattcaggcg attttgagaa
    11341 actggcgcca tgaaggaatc aagagtgtgg ctcaaattga ggccaagaga gcagaaagag
    11401 aagcaagcaa tcctcagttg acacaggtat ctgcagattt cagaaatgcc atggatctct
    11461 ggaaggatta atccatgcaa gtaggcttga aatccgagta agatttgcaa gctgtgtata
    11521 attgtgatag aataaataga aaataaattg aaaaaagagg tatgtgaaat gtcacgtaaa
    11581 ccatttatcg ctggtaactg gaaaatgaac aaaaatccag aagaagctaa agcattcgtt
    11641 gaagcagttg catcaaaact tccttcatca gatcttgttg aagcaggtat cgctgctcca
    11701 gctcttgatt tgacaactgt tcttgctgtc gcaaaaggct caaaccttaa agttgctgct
    11761 caaaactgct actttgaaaa tgcaggtgct ttcactggtg aaactagccc acaagttttg
    11821 aaagaaatcg gtactgacta cgttgttatc ggtcactcag aacgccgtga ctacttccat
    11881 gaaactgatg aagatatcaa caaaaaagca aaagcaatct ttgcgaacgg tatgcttcca
    11941 atcatctgtt gtggtgaatc acttgaaact tacgaagctg gtaaagctgc tgaattcgta
    12001 ggtgctcaag tatctgctgc attggctgga ttgagtgctg aacaagttgc tgcctcagtt
    12061 atcgcttatg agccaatctg ggctatcggt actggtaaat cagcttcaca agacgatgca
    12121 caaaaaatgt gtaaagttgt tcgtgacgtt gtagctgctg actttggtca agaagtcgca
    12181 gacaaagttc gtgttcaata cggtggttct gttaaacctg aaaatgttgc ttcatatatg
    12241 gcttgcccag acgttgacgg tgcccttgta ggtggtgcgt cacttgaagc tgaaagcttc
    12301 ttggctttgc ttgactttgt aaaataatca gtaagtagca aaagctaggt ggaacagcat
    12361 tcagatgtct gttacatttt ttataggaga ggaaagattg aaaataaaaa ttggattagc
    12421 aagtatctgt ttactaggct tggcaactag tcatgtcgct gcaaatgaaa ctgaagtagc
    12481 aaaaacttcg caggatacaa cgacagcttc aagtagttca gagcaaaatc agtcttctaa
    12541 taaaacgcaa acgagcgcag aagtacagac taatgctgct gcccactggg atggggatta
    12601 ttatgtaaag gctgatggtt ctaaagctca aagtgaatgg atttttgaca actactataa
    12661 ggcttggttt tatattaagt cagatggtcg ttacgcgcag aatgaatggc atggaaatta
    12721 ctacctgaaa tcaggtggat atatggccca aaacgagtgg atctatgaca gtaattacaa
    12781 gagttggttt tatctcaagt cagatggggc ttatgctcat caagaatggc aattgattgg
    12841 aaataagtgg tattacttca agaagtgggg ttacatggct aaaagccaat ggcaaggaag
    12901 ttatttcttg aatggtcaag gagctatgat gcaaaatgaa tggctttatg atccggctta
    12961 ttctgcttat ttttatctaa aatccgatgg aacttatgcc aaccaagagt ggcaaaaagt
    13021 gggcggcaaa tggtactatt tcaagaagtg gggctatatg gctcggaatg agtggcaagg
    13081 caactactat ttgactggaa gtggtgccat ggcgactgac gaagtgatta tggatggtgc
    13141 tcgctatatc tttgcggcct ctggtgagct caaagaaaag aaagatttaa atgtcggctg
    13201 ggttcacaga gatggtaaac gctatttctt taataataga gaagagcaag tgggaaccga
    13261 acatgctaag aaagtcattg atattagtga gcacaatggt cgtatcaatg attggaaaaa
    13321 ggttattgat gaaaatgaag tagatggtgt cattgttcgt ttgggctata gtggaactga
    13381 ggacaaggaa ttggctcata atattaagga gttaaaccgt cttggaattc cttacggtgt
    13441 ttacctttat acttatgctg aaaatgagac cgatgctgag aatgatgcta atcaaaccat
    13501 tgaactcttg aaaaaataca agatgaactt gtcttaccct atctactacg atgttgagaa
    13561 ctgggaatat ggcaataaga ccaagagggc tccaagtgat actggaacct gggtcaagat
    13621 tatcaacaaa tatatggcaa caatgaagca ggcgggttat caaaatgtgt atgtctatag
    13681 ctatcgtagt ttattacaga cgcgtttaaa acacccagat attttaaaac atgtaaactg
    13741 ggtagcggcc tataccaatg ctttagaatg ggaaaaccct cattattcag gagaaaaagg
    13801 ttggcaatat acctcttctg aatacatgaa aggaatccaa gggcgcgtag atgtcagcgt
    13861 ttggtattaa gcgatgattt gaaagaggga tgtgatacta gcaccctctt tttctttgtt
    13921 ttatgatagt ttatcctcga gtaaattcaa gaacttgctc ggaaatgaag tttatgttta
    13981 tatagtatat tgaagttaga atagtacacc ttgacttcta aaacattgtt agaaattgat
    14041 ttgactatcc taatcaattc gtcttattct tatttcattt tactatagat tgaatataga
    14101 ccaggacatt gcggttgcta aaacattttt agaaattcat ttgtctttcc taatcgactt
    14161 ggttccctct tatttcaatc tattatagtc ttggggaatt tcttcaaacc acatcagcgt
    14221 gctcagttct acctgcgacc tcaaaacttg tgctttggtc aagatgggtt tggcttccta
    14281 gtttgctgat ggatttccat ggactataag catccagacc tctttttcgt cttctaaaga
    14341 attcttaaat tatcagtcta ttgcaacttt tctcatgtgg gtcatttggc ttgctattat
    14401 ctttccttag tagtatacta aggtagtaat cattaagaag tggttacata aaaataatga
    14461 atgaggtaaa ataaaatgat agaattgaaa aaagaagcag caaaagatgt cgcaacatta
    14521 acaaaagcag cgccagtagc attggcaaaa acaaaggaag ttttgaatca agctgttgca
    14581 gatttgtatg tagcgcatat tgctcttcat caagtgcact ggtacatgcg tggacgcggt
    14641 tttatggtat ggcatccaaa aatggatgag tacatggatg ctcttgatgg tcatttagat
    14701 gaaatcagcg aacgattgat tacactcgga ggtagcccat tctctacatt gacagagttc
    14761 cttcaaaaca gtgaaatcga agaagaagtt ggagagttcc gcaatgtaga agaaagtttg
    14821 gaacgtgtcc ttgaaattta tcgttacctc atcacgcttt tccaagaagc tttggatgtg
    14881 acagataaag aaggcgatga tgtaaccaat gatatctttg ttggagctaa ggctgacctt
    14941 gaaaaaacag tctggatgct tgcggcagaa ttaggacaag cacctggttt gtaagaaaca
    15001 atagaaaata tataaatctg taggaaattt cttacagatt ttttctattc tgtaagctat
    15061 tccaaaccag tcttttttcg agttttttga taaaatagta ctatcaatga aaaggatgga
    15121 agcatgacta agaaaatcgt agctatttgg gcccaggatg aagagggtgt gattggtaag
    15181 gacaatcgtc tgccttggca tttgccagca gaattgcaac actttaaaga aacaactctg
    15241 aatcatgcca tcttgatggg gcgcgtgacc tttgatggaa tggggcgtcg cttgcttcca
    15301 aagcgggaga ccttgatttt gacgcgtaat tctgaagaaa agacagacgg ggttgctact
    15361 tttcacgata tccagtctgt cttagattgg tatcaggctc aagacaagaa tctttatatt
    15421 ctcggtggaa agcagatttt tcaggctttc gaaccttacc ttgatgaagt gattgtgact
    15481 caaattcatg ctcgggtgga gggagatacc tattttcctg aggagtttaa cttgtctctt
    15541 tttgagacag tttcaagcaa attttacgcc aaagatgaga aaaatcctta tgattttacc
    15601 atccaatacc gcaagagaaa ggaagtctaa tggaacggag tttatttggt ttattcacgg
    15661 cttttctgtg tttcatctgc cttttggcag gagcacaggc ttttcgtaaa aagcgttacg
    15721 ggctttctat cctcctttgg ttaaatgcct ttaccaatct ggtcaatagt attcatgctt
    15781 tttacatgac cttattttag atagaatgac agtatagaat ggaaggaaat catgcctaca
    15841 actagaaaaa atgatatgat ggtttattgc tcattttgtg gcaaaaacca agaagaagta
    15901 caaaaaataa tcgctggcaa caatgccttt atttgtaatg aatgcgtgga gttagctcaa
    15961 gaaattattc gagaagaatt ggttgaggaa gtcttggcag acttgtctga ggtgccaaaa
    16021 ccaattgaac tcctccatat cttgaaccac tatgtaattg gtcaagatcg tgccaagcgt
    16081 gccttggcag tggcggttta taaccactac aaacgcatca atttccacga tacacgcgaa
    16141 gagtcagaag atgtggattt gcagaagtca aacattttga tgattggccc aactggttca
    16201 gggaaaactt tccttgccca gaccttggct aagagcttga atgtaccttt tgctattgcg
    16261 gatgcgacag ctctgacgga ggctggttat gtgggtgagg atgtggaaaa tatcctcctc
    16321 aaactcttgc aggttgctga ctttaacatc gaacgtgcag agcgtggcat tatctatgtg
    16381 gatgaaattg acaagattgc caagaagagt gagaatgtgt ctatcacacg tgatgtttct
    16441 ggtgaagggg tgcaacaagc ccttctcaag attatcgagg gaactgttgc tagcgtgccg
    16501 ccacaaggtg gacgcaaaca tccgcaacaa gagatgattc aagtggatac taaaaatatc
    16561 ctcttcatcg tgggtggtgc ctttgatggt attgaagaaa ttgtcaaaca acgtctgggt
    16621 gagaaagtta ttggatttgg tcaaaacaac aaggcgattg acgaaaacag ctcctacatg
    16681 caagaaatca tcgcagaaga tattcaaaaa ttcgggatta tacctgagtt aatcggacgt
    16741 ttgcctgtct ttgcggctct tgagcaattg accgttgatg acctggttcg catcttgaaa
    16801 gaaccaagaa atgccttggt caaacaatac caaactttgc tttcttatga tgatgtcgag
    16861 ttggaatttg acgacgaagc ccttcaagaa attgccaata aggctatcga acggaagaca
    16921 ggtgcgcgtg gacttcgctc catcatcgaa gaaaccatgc tagacgtcat gtttgaagta
    16981 ccgagtcagg aaaatgtgaa attggttcgc atcactaaag aagctgtcga tggaacggat
    17041 aaaccaatcc tagaaacagc ctagaggtga ctatggaact gaatacacac aatgctgaaa
    17101 tcttgctcag tgcagccaat aagtcccact atccgcagga tgaactgcca gagattgccc
    17161 tagcagggcg ttcaaatgtt ggtaaatcca gctttatcaa tactatgctg aaccgtaata
    17221 atctagcccg tacatcagga aaacctggta aaacccagct tctcaacttc tttaatatcg
    17281 atgacaagat gcgttttgtg gatgtgcctg gttatggtta tgcccgtgtt tctaaaaagg
    17341 agcgtgaaaa gtgggggcgc atgattgagg agtacctaac gactcgggaa aatctccgtg
    17401 cagtagtcag tctagttgac ctccgtcacg acccgtcagc agatgatgtc cagatgtacg
    17461 aatttctcaa gtattacgag attccggtta tcatcgttgc gaccaaggcg gacaagattc
    17521 cgcgtggtaa atggaataag catgagtcag caatcaaaaa gaaattaaat tttgacccaa
    17581 gtgatgactt catcctcttt tcatctgtca gcaaggcagg gatggaccaa gcttgggatg
    17641 caatcttaga aaaattatga ggtaaagaaa atggcaaaaa caattcatac agataaagct
    17701 ccaaaggcta ttggccccta tgttcaagga aaaatcgttg gcaacctttt gtttgctagc
    17761 ggtcaagttc ctctctctcc tgaaactggg gaaatcgttg gagaaaccat tcaagaacag
    17821 acagaacaag tcttgaaaaa cattggtgct attttggcag aggcaggaac agactttgac
    17881 catgttgtca aaacaacttg cttcttgagc gatatgaacg actttgttcc ttttaacgag
    17941 gtttaccaaa cggccttcaa agaggaattc ccagctcgtt cagctgtaga ggtagctcgt
    18001 cttcctcgtg atgtaaaagt cgaaatcgaa gtcatcgcag agattggata agctagttga
    18061 agtttggttc tgccaaactt cttttgatat aaggagaaaa agatgacaaa gaaacaactt
    18121 cacttggtga ttgtgacagg gatgagtggg gcaggaaaaa ctgtagccat tcagtccttc
    18181 gaggatctag gttatttcac cattgataat atgccgccag ccctcttgcc taagtttttg
    18241 cagctggttg aaattaagga agacaatcct aagttggcct tggtagtgga tatgcgtagc
    18301 cgttctttct tttcagagat tcaagctgtt ttggatgagt tggaaaatca agatggtttg
    18361 gatttcaaaa tcctcttttt ggatgcggct gataaggaat tggtcgctcg ttacaaggaa
    18421 accagacgga gtcacccact agcagcagac ggtcgtattt tagatggaat caagttggaa
    18481 cgtgaactct tggcaccttt gaaaaatatg agccaaaatg tggtggatac gactgaactc
    18541 actccacgtg aactgcgcaa aacccttgca gagcagtttt cagaccaaga acaagcccag
    18601 tctttccgta tcgaagtcat gtctttcgga tttaagtatg gaatcccgat tgatgcggac
    18661 ttggtctttg atgtccgttt cttgccaaat ccctattatc taccagaact gagaaaccaa
    18721 acgggtgtgg atgaacctgt ttatgattat gtcatgaacc atcctgagtc agaagacttt
    18781 tatcaacatt tattggcctt gattgagccg attctgccaa gttaccaaaa ggaaggtaag
    18841 tccgttttga ccattgctat gggatgtacg ggtggacaac accgtagtgt ggcatttgct
    18901 aaacgcttgg cgcaggactt atccaagaat tggtctgtta atgaagggca tcgcgacaaa
    18961 gaccgcagaa aggaaacggt aaaccgttca tgagaaaacc aaagataacg gtgattggtg
    19021 gagggactgg aattcccgtc attctaaaaa gtctgcggga aaaagatgtg gaaatcgcag
    19081 ctatcgtgac ggtggcagat gatggtggtt cttcaggtga actccgaaaa aatatgcaac
    19141 agttgacacc gccaggtgat cttcgtaatg ttcttgtggc catgtcggat atgcctaagt
    19201 tttatgagaa ggtctttcag tatcggttct ctgaggatgc cggagccttt gctggccatc
    19261 cattgggaaa tctcatcatt gctggcttgt cagaaatgca gggttcaacc tataatgcca
    19321 tgcagttatt gagcaaattt ttccatacaa cagggaaaat ttatccttcc agtgaccatc
    19381 ctttgaccct tcatgcagtc tttcaggatg ggacagaagt ggttggagag agtcatattg
    19441 tagaccatcg aggcataatt gacaatgtct atgtgaccaa tgccctaaac gatgatacgc
    19501 ctctggccag ccgtcgagta gtgcagacca tccttgaaag tgacatgatt gtcctagggc
    19561 caggttccct ctttacctct attttgccca atatcgtgat taaggaaatt gggcgggctc
    19621 ttttggaaac taaggcagaa attgcctatg tctgcaatat catgacccaa cgtggggaga
    19681 cggaacactt tacagatagc gaccacgtgg aagtcttgca tcgtcacctt ggtcgccctt
    19741 ttatcgacac tgtcttggtg aatattgaaa aagtgcctca ggaatacatg aattccaacc
    19801 gttttgatga atacttagtg caagtggaac acgattttgt aggtctttgt aagcaagttt
    19861 cgcgcgtgat ttcatctaac ttccttcgtc tggaaaatgg cggtgccttc cacgatggag
    19921 atttgattgt ggacgagttg atgcgcatta tacaggtgaa aaaatgagtt tcacagtagc
    19981 agtaaaagaa gaaatcctgg gccaacacca tctgagccgg catgaattat ctgccattat
    20041 caaaatgtct ggtagcatcg gtctctcgac ttcgggcttg actttgtcag ttgtgacaga
    20101 aaatgccaaa ctggcccgtc acctctatga gtcctttctc catttctatg aaatcaaatc
    20161 tgaaattcgt caccaccaac ggagcaatct tcgcaagaat cgcgtctata ccgtttttac
    20221 agatgaaaag gtgcaggatt tgttaagtga tttgcacttg gcagactctt tctttggcct
    20281 ggaaacaggt attgatgagg cggttttatc ggacgaggaa gcaggtcgta cctatctctg
    20341 tggcgctttc ttggcaaatg ggagcattcg tgaccctgag tcaggcaagt accagttgga
    20401 aatcagttct gtttatctgg accacgcgca aggaattgcc tcccttctcc aacagttttt
    20461 actggatgcc aaggtgcttg agcgcaagaa gggggctgtg acctatctcc agcgagcaga
    20521 agacattatg gacttcttga tagtaatcgg agccatgcag gcacgtgatg attttgagcg
    20581 ggttaagatt ttgcgagaaa cccgtaacga cctcaatcgg gctaataatg ccgagacagc
    20641 taatatcgct cggacagttt ctgccagcat gaagaccatt aacaatatca gtaaaatcaa
    20701 agatatcatg ggcttagaaa atttgccagt agatttgcag gaagtggcgc aactgcggat
    20761 tcagcaccca gactactcta tccaacagtt ggcagatagc ctcagcaccc ctctgaccaa
    20821 aagtggtgtc aatcacagac tcagaaaaat caataagata gcgga
//
LOCUS       DAVKDZ010000031        20740 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000031 DAVKDZ010000000
VERSION     DAVKDZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20740
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.032"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            94..1548
                     /locus_tag="VWZ30_001141"
     CDS             94..1548
                     /locus_tag="VWZ30_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9334896.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLIHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDKNWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFQGQYYAIVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFIEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNCSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSHAGEQYAQEFGITRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            1529..2494
                     /locus_tag="VWZ30_001142"
     CDS             1529..2494
                     /locus_tag="VWZ30_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9334897.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(2530..3804)
                     /locus_tag="VWZ30_001143"
     CDS             complement(2530..3804)
                     /locus_tag="VWZ30_001143"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU9334898.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEQDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKHVGGFT
                     AQVHQRQMIGQVSLYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSSQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVEQLIADKTFNLETAQRYLENLRS"
     gene            complement(3804..4976)
                     /locus_tag="VWZ30_001144"
     CDS             complement(3804..4976)
                     /locus_tag="VWZ30_001144"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU9334899.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKNLPQEKKDLLQNHFDHSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTDLSVADYEKVFFEEVDLDETNSAQFAGYENQYFALVEILDHQRRYSKVEK"
     gene            complement(5107..5355)
                     /locus_tag="VWZ30_001145"
     CDS             complement(5107..5355)
                     /locus_tag="VWZ30_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334900.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QEFVDEMGRTYSGNLILQELITVNEAYKATYIGELYIN"
     gene            complement(5423..6047)
                     /locus_tag="VWZ30_001146"
                     /pseudo
     CDS             complement(5423..6047)
                     /locus_tag="VWZ30_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6109..6558)
                     /locus_tag="VWZ30_001147"
     CDS             complement(6109..6558)
                     /locus_tag="VWZ30_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9334901.1"
                     /translation="MTLTTFLLQAVASLLAIITFLIVLNVQRSMLLPGGILGMAVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGIASGTVILRLYHYIKTHRVS"
     gene            complement(6560..7318)
                     /locus_tag="VWZ30_001148"
     CDS             complement(6560..7318)
                     /locus_tag="VWZ30_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9334902.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSIMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGMIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(7433..9412)
                     /gene="pknB"
                     /locus_tag="VWZ30_001149"
     CDS             complement(7433..9412)
                     /gene="pknB"
                     /locus_tag="VWZ30_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU9334903.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVMKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRKSSDVIAELKEKKVPDNLIKIEEEESSESEPGTIMKQSPGAGTTYDVN
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVSTAPAGTAE
                     GTVVEQSPRAGEKVDLNKTRVKISIYKPKTSSATP"
     gene            complement(9409..10149)
                     /locus_tag="VWZ30_001150"
     CDS             complement(9409..10149)
                     /locus_tag="VWZ30_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU9334904.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(10164..11477)
                     /gene="rsmB"
                     /locus_tag="VWZ30_001151"
     CDS             complement(10164..11477)
                     /gene="rsmB"
                     /locus_tag="VWZ30_001151"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU9334905.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKA
                     LVTELVYGTVARRLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(11470..12405)
                     /locus_tag="VWZ30_001152"
     CDS             complement(11470..12405)
                     /locus_tag="VWZ30_001152"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU9334906.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELIKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGALFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(12418..14814)
                     /locus_tag="VWZ30_001153"
     CDS             complement(12418..14814)
                     /locus_tag="VWZ30_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU9334907.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDEEEMEQDLKDIAEVLDFSPVLTQEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLGKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHVQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGTLDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEVAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSHSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLCYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGLTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(14880..15194)
                     /locus_tag="VWZ30_001154"
     CDS             complement(14880..15194)
                     /locus_tag="VWZ30_001154"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU9334908.1"
                     /translation="MMLKPSIDTLLDKVTSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(15219..15845)
                     /gene="gmk"
                     /locus_tag="VWZ30_001155"
     CDS             complement(15219..15845)
                     /gene="gmk"
                     /locus_tag="VWZ30_001155"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU9334909.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQERLVGRGTDSAEVVAQRIEKAKEEIALM
                     REYDYAIVNDQVSLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(15975..17588)
                     /locus_tag="VWZ30_001156"
     CDS             complement(15975..17588)
                     /locus_tag="VWZ30_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU9334910.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERRKEAE
                     LERIGALSQAEARDIILAQTEENLTKEIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(17712..20699)
                     /locus_tag="VWZ30_001157"
     CDS             complement(17712..20699)
                     /locus_tag="VWZ30_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEU9334911.1"
                     /translation="MANLGLKERYDFQQWIIKDLRDKNDYIQRDATCYDPRLAMDVEL
                     LWDFLMETQEETMDYLLKKLSKETIVNLINKEIVKGGFLFALKEGVFIENKQLRLLYR
                     KPATSFNEKAVQQYQVNCLSIMEEVVYEESDRIDLVIFINGLALFAIELKFNPSGQSV
                     QDAIRQLKGRNPKNRLFRFEQGVLASFAVDTLEVYMTTQLKGQETYFLPFNVGKGEGI
                     EMGSGNPHNPDGVDTDYLWLDVLTKDSILQLIDSFIFFEMDEMDKHNSKKKTLIFPRY
                     HQRRAVSRLLEDMKVNHTDSNYLIQHSAGSGKTNTIAWLAHSLVSLHDEQNQNIVDTV
                     LIVTDRIVVDRQLQEAVKEISHKPGVVKVMGDKETSSDLADAIAGNTKIIATTIHKFA
                     QINQSNWMSVGNTAKKKFAILIDEAHSSTTGSYMSSVSQVLTETDTEDGTIDEIQEVS
                     VADAIEQEIARTGKQDNVSIIAFTATPKATTLQLFGTTQADGSKAPFDVYSMKQAIEE
                     GFILDVLDNYTTYKTYYKLNKAVEEDPELKTTLAKRKIAKFVELSDENINQKVEIIMD
                     HFISHDIMAELGGQGKAMIVTSGREAAVKYQLAFQKYFKDHSITSIGTLIAFSGKVDY
                     QGSEYTESQMNQVKEDYLKDHFDTEDYQILIVANKYQTGFDQPKLVAMYVDKKLRSVA
                     AVQTLSRLNRIYRGYNKRTFILDFKNTYDDIRSAFSPYYRETILADTVTPSDVLTLDR
                     KIDEYGILDSDVIHEFNQFLYQEKRNSREKQKMVSLLNQGYDRVRRFDEKEQLSIRKV
                     IRGFLRMYTFLIQATAYQNEVLHERYNYLQSLVKMIDVRIGGDDFTIADKIVVDYMKH
                     KQTGSFRSVSELEYHPELILTKPSTGGVTTDQEKRLSEILDEINEQLDLDIDPQVGLS
                     GAVSIRELMKKNPRLKQSARVNSREDFVFAFEEEIDNALIEGYSQSQDLFGALLNNDS
                     FKKRLADIFIDDVFKSLKGSEEV"
BASE COUNT         5711 a         4839 c         4039 g         6151 t
ORIGIN      
        1 tttgacagtt ttttctcttt tttaatataa aaggcttgca ttttttatcg aaaacagtta
       61 ctattaaaag tgataagatt tttggaggaa tgaatggaat ggacaactga gcgtcgttac
      121 agactttatc aagattggac gcaagaagaa attcaacata taaaggaaaa tatggcacaa
      181 tctccatggc atactcatta ccatgttgag ccaaaaacag gacttctcaa cgacccaaat
      241 ggcttttctt actttgatgg caagtggatc ctcttttacc agaattttcc ttttggtgca
      301 gcccacggtt taaaatcttg ggcacagcta gaaagtgatg atttgattca ctttaaagaa
      361 actggaatca aagttttacc agatactcca ttagatagcc acggtgccta ctctggttct
      421 gccatgcaat ttggcgataa cttattccta ttttatacag gaaatgttcg cgataaaaac
      481 tggatccgtc acccatacca gatcggtgct ttgatggaca aggagggtaa gattacaaag
      541 attgacaaga tcttgattga ccagccagca gactctactg accacttccg cgatccacaa
      601 atttttaact ttcagggtca atattatgcc attgtcggcg gacaagactt ggagaaaaaa
      661 ggtttcgttc gtctctacaa ggctgtcaat aacgactaca caaactggca agcagttggc
      721 gaccttgact ttgctaacga ccgtactgcc tacatgatgg aatgtcctaa tctggtcttt
      781 atagaggaac aacctgtcct tctctactgt ccacaaggat tggataagaa agttctagac
      841 tacgataata tctttccaaa tatgtataag atcggggctt cctttgaccc taaaaatgcc
      901 aaaatggtag atgtgtctca acttcaaaac atggattacg gtttcgaagc ctatgcaact
      961 caagccttca acgctcctga tgggcgtgct ctagcagtta gctggcttgg tttgccagat
     1021 gtttcttacc catctgaccg ttttgaccac caaggaacct tctctttggt caaggaactc
     1081 actatcaaag acgacaagct ctaccagtat ccagtcgctg ctattaagga ccttcgtgct
     1141 tctgaagaag ccttctcaaa ctgttcccaa accaagaaca cttacgaact tgaactcaac
     1201 ttggaagcta atagccagag cgagattgtc ttacttgctg ataaagaagg taagggactt
     1261 tcaatcaact ttgaccttgt aaacggtcaa gtgacagtgg atcgtagcca tgctggagaa
     1321 cagtatgccc aagaatttgg gataactcgt tcttgcccta tcgagaatca ggctactact
     1381 gctacaatct tcatcgataa ctctgtcttt gaaattttca tcaataaagg agaaaaagta
     1441 ttttctggtc gtgtcttccc acatgcggac caaaatggta tcctgatcaa atctggaaac
     1501 ccaactggaa cttactatga attagattat ggtcgcaaaa ctaactgatg tcgccaaact
     1561 tgcaggcgtc agtcctacta ccgtttctcg ggttatcaat aaaaaagggt atctatctga
     1621 gaaaaccatc caaaaagtca atgaagccat gcgagaattg ggctataaac ccaacaacct
     1681 ggctcgtagt ctgcaaggaa aatcagctaa gttaatcggc ttgattttcc ccaatatttc
     1741 caatgttttc tatgcagaat tgattgataa attggaacac caactcttca aaaatggtta
     1801 caagaccatc atctgcaaca gtgaacatga ttctgagaag gaacgtgaat acatcgaaat
     1861 gttggaagcc aatcaggtgg acggcatcat ttctggtagt cacaacctag gaatcgaaga
     1921 ctacaatcgt gtgacagcgc cgattatttc ctttgaccga aacctatcgc cagacatccc
     1981 tgtcgtctcc tctgacaact atgctggtgg ggttcttgct gcccaaacct tggtcaagac
     2041 aggtgcccag tctatcatca tgattacagg gaatgacaat tccaattcgc caaccggact
     2101 gcgccacgct ggttttgcat ccgtactccc aaaagctcct attatcaatg tttccagtga
     2161 cttttctccc gtcagaaaag aaatggaaat caagaatatc ttgacccggg aaaaaccaga
     2221 tgccattttt gcttcggatg atttgacagc tattctggtc attaaaatcg ctcaagaatt
     2281 gggcatttct gtcccaaaag agctcaaggt catcggctat gatgggacct actttatcga
     2341 aaattactac cctcaattgg ctactatcaa gcaacctttg gaagagattg cttgtctcac
     2401 tattgatctt ctcttgcaaa agattgaagg caaggaagtc gccacaactg gttacttctt
     2461 accagttaca ctattaccag gaaaaagtat ttaaacacaa gaaaactcag accgattcgt
     2521 ctgagttttt tatgatctta aattttcgag atagcgctgg gctgtctcta ggttaaaggt
     2581 tttatctgcg ataagctgct cgactagggg agcaacttca gattcactag ccccagctag
     2641 gagagctagg gatttggcct gtagtttcat gtggccttgc tggatgcccg tacttaccaa
     2701 ggctttgagg gctgcaaaat tttgggcaag accgatggac acgataatct gggctaattc
     2761 tctggcagaa ggatttccta gtagatcatg actgagagct acacgtgggt tgaggccgat
     2821 agagccaccc ttagtcgcta caggcatggg cagggtcatc tcaccgacca attcttctct
     2881 ttcaaggtcc agcgtccagc aactaagacc ttgataacgt ccatctcgac tggcaaaggc
     2941 atgggcccca gcttcgatgg cacgccagtc attaccagtg gcaatcaaaa tcgcatcaat
     3001 accattaaaa attcctttat tatgagtagc agctcggtaa ggatcagcct gcgcaaactg
     3061 actagccaac gcaattttct ccgcaatctc tcgtccttga tccttttggc tgctcaagta
     3121 gcgaaaggcg atgcgacagc ttgcagtcac cagagaatcg gtcgcgtagt tggacaggat
     3181 tcccatgaga ctctgtccct gactgagttc ttctaagact ggtttcaagg cttccagcat
     3241 ggtgttgagc atattggcac ccatggcttc ctgggtatcg acatgaatat aaacaacgag
     3301 aaagtctggt tcacctttta tctgctcgac atgcaggtca cgcgccccac ctccacgttt
     3361 aacgatagaa ggataggctt gattggcaag ctccaagagc tccgctttct tgctggcaat
     3421 cttctcttgc gctagtttag gattagcaac ttgataaagg gatacctgcc caatcatctg
     3481 gcgctgatgg acttgtgcag taaaaccacc tacatgcttg atgattttgc tggcatagct
     3541 ggccgccgca accacagagg gttcttctgt cacataggga acggtgtatt cctgaccgtt
     3601 gacaagtacc tccggaacca gcgaataagg cagagaaaaa gttcccacca cattctcact
     3661 cagctggtct gccacagtca cactcatctg ttcatcctgc tccagactag cttgtctctc
     3721 aggactaagg agcgcctgag cttttaacag ctcgaggcgc tcttggtatg attttttaga
     3781 aaatccattc caacttatct tcattatttt tcaaccttgc tataacggcg ttggtggtcg
     3841 agaatttcaa ccaaggcaaa atattgattt tcatagccag caaactgggc agagtttgtt
     3901 tcatccaagt ctacttcctc aaaaaagacc ttttcatagt ctgcaacgga taggtcagtt
     3961 cgttggttga gcttgttcaa gcggtcttta tccaaataag cttcatatcc ttcaaccaat
     4021 tcaccactga agaactcagc cacagctcca cttccgtaac tataaagggc gattttatcc
     4081 ccagctttca agctatctgt attttccaag agagacaaaa gtccaaggaa aagtgaacct
     4141 gtgtagatat tccccacctt ttgactatag agaatagaat ggtcaaaatg gttttgcaag
     4201 aggtcttttt tctcttgagg caagttctta tccataattt ttttcaagcc ttttagcgct
     4261 aatttaggat aaggcaagtg gaagcaaacc gccgcaaaat catctaaggt aagctggtag
     4321 cgtttttggt attcaagcca agtcgttttc aaactatcca agtattgttg ggtagaatag
     4381 acaccattta cataaggagt tgtcgagtaa tttggtcgcc agaaatccat gatgtcacgg
     4441 gtctgagcta cattgtcatt attaaaggcc atcatgcgtg gattttgtgt aatcaacata
     4501 gccacacttc cagcaccctg agttggttct cctggagttt caattccata cttggcaata
     4561 tcactggcga tgaccaagac tttcgactct ggagaatttt ccacatgcaa tttggcataa
     4621 tgaagggcag cagtcgctcc atagcaggct tctttaatct cgaaactacg agcaaagggc
     4681 tggatgccca gcaagccatg cacaaagacg gccgcagcct tactctggtc aattcctgac
     4741 tcggtcgcca caatgaccat gtcaacttct tgtctttctt gctcagttaa aatagagtca
     4801 ctagcactgg ccgccaaggt cacgatgtcc tcagttaggg gcgcaatact caattccttg
     4861 agtaagagtc ctttacttaa tttttcaggg tcaattcccc tcgcttctgc taagtcttgt
     4921 aatttcaaga catattgact ggtcgcaaaa ccaatcttat caataccgat tgtcatattt
     4981 acctctgttt tatcattcat gtaaaaaatc gttctatact attttatcac aaatggcagt
     5041 aaaagagaga aaaaagactt gattcaccaa atcaagcctc ttattggtca taattttaaa
     5101 gaatgattag ttgatataga gttcaccgat ataagtagct ttataagctt cattcacagt
     5161 tatcagctcc tggaggatca aatttcctga gtaagtcctt cccatctcat ctacaaattc
     5221 ttgataaaac tgactggtcg gaatttctct gacatcctta tcaaatgtct tgtcaagtgt
     5281 tttactaacc ttctcagcaa tcaattgatg ctcttgccat ccactttgaa actctgagcc
     5341 cgaactagaa accatgactg ggataaacaa caaggtccat agacttacag acaataagga
     5401 aaatagtaga cttcctgcaa aactagaatc ctagttcatg attgataata tcagcaatca
     5461 aattcattcg taatccgaag cgtttacgat gatttcgata ggttgttgaa atcattttaa
     5521 acgtttttac tttggcaaag atgttctcaa ccttgcttct ctccttggat agcgcatggt
     5581 tacaggcttt atcttcaact gttagcggct tgagtttgct ggatttacgt ggagtttgta
     5641 cttgaggata tatcttcatg agcccttgat aaccactgtc agccaagatt ttaccagctt
     5701 gtccgatatt tttgcgactc attttgaaca acttcatatc atgacaatag ttcacagcga
     5761 tatccaaaga aacaattctc ccttgacttg tgacaatcgc ttgagccttc atagcgtgaa
     5821 atttcttttt accagaatca ttcgctaatt gtttttttag ggcgattgat ttttacttcc
     5881 gtcgcatcaa tcattaccgt gtcctcagaa ctgagaggag ttcttgaaat cgtaacacca
     5941 ctttgaacaa gagttacttc aacccattgg ctccgacgga gtaagttgct ttcgtggata
     6001 ccaaaatcag ccgcaatttc ttcataagtt cgatattctc gcacatatag aaagcgttgt
     6061 tactatattt atctcatttt tcagaaaatt cttttatttc tgtaaaatct acgatactcg
     6121 atgtgttttt atataatgat acagtctgag aatcactgtt ccgctagcta ttccaataga
     6181 gattaccaaa gccagcatga caaccaaggt cgcacttgcc agcgctttat tatagtctcc
     6241 tgtcacaaaa aaggcagttg tccgataaga aaggtagccc ggaaccagcg gtgccaaaat
     6301 ggccaagata aagaccacag caggagtctt ataaagaata cttaaaatct ggctgacaca
     6361 agaaccgatt atggcggcaa taaaggtagc cacaataaca ttggtcggtt ccttgagcaa
     6421 gagataaatc agccagacag ccatgcccaa aatccctcca ggtaagagca tagagcgttg
     6481 cacattgagt acgattagaa aagtgataat ggcaagaaga cttgctactg cttgtaataa
     6541 aaaggttgtt agtgtcatat tagttcatca ataccaaggc gacagaagtt cctgccccta
     6601 aagcaagggt aatgagcaga gattcaaaca tcttgctcat accagagttt atgtggttgg
     6661 tcataatatc acgaaccgca ttggtcaagg caatccctgg tacaaacggc atgaccgcac
     6721 cagctataat caaatctgct gttgaaggaa agcctgtgta gcgagcccaa aactgggcaa
     6781 tcatcccaaa gacaaaggct ccagcaaagg ctgtcacaaa gggaattcgg ataaacttct
     6841 ccacatagag ggaaaaggca aagccaaata aagtagccac tcctgcccca agtgcgtcgt
     6901 aaatatttcc actaaacata attgaaaaga aaggagcact aaaggtcgca gccagagtta
     6961 cctgcaactt agtataggga aggggttggg cttgcaaggc cgtcaattgc ttaaaggctg
     7021 tttctaaatc aatctgcccc ccaaccagct gacgagaaat ctggttcaca tcgcagactt
     7081 tttcaatgtt ataagaagag gaggtcacgc gcttcatgcg cgaaatatta gtattttcaa
     7141 tagagaaaaa gatagcagca ggcatggcaa gaacattgca atccacaatc ccctgcgaat
     7201 gcgcgatccg aatcatggta tcttctacac gatggatttc tgagccactt ttaaggagaa
     7261 tagtccccgc tagcataatc acatcgatga cggcatttaa ttcttttgac tcttccatgc
     7321 ttgcctcctt ttatcaactc cctctattct atcacaaatc cggactcaaa aaaaatcttt
     7381 gccacaaaat catgacaaag attgattact cattttgatt atccatctga ttttaaggag
     7441 tagctgaact tgttttaggt ttgtagattg aaatcttgac tctagtctta ttgaggtcta
     7501 ccttttcacc tgctctagga ctctgttcaa cgactgtacc ttcagcagtt ccagcgggag
     7561 ctgtcgacac ttctacaact tcaatattgg cttccttaat cccaacaatt tgaatcaaat
     7621 tattcttagt aaactccaag ctggaaccaa tgtagctcgg catggcaaca cttgtaactt
     7681 ttttagctac tgtcaagacg atttgggtag gtttattcac atcataagtc gttccggcac
     7741 ctggactttg tttcataatc gttcctggtt cgctttcgct ggactcttct tcctctatct
     7801 taatcaaatt atctggaact tttttctctt ttaattccgc aataacatca gaggatttcc
     7861 gtccaatata gttccctaat tgaatcgtcg tagctttttt agctactgtc aagacgattt
     7921 gagttgcctt gctcaagtca taggtcgtac cttctggtag actttgcttc atgaccgttc
     7981 cagcctcact ctcattcgac tcttcttcct caattttaat caaattatct ggaacttttt
     8041 tctcttttaa ttccgcaatg acatcagagg atttccgacc gacataatta ctaatttgga
     8101 aggattgttt gcctgatgag acaaccaaat tgattttcgt tccttctttt cgaccagttc
     8161 cagcgccagg atctgtacgg ataatccgcc cttcttccac cttttcacta gcctctgtct
     8221 tctcctcacc aatctcaaaa ttggcttttt tgagcgttgc cttggcctct gcaactgtct
     8281 gacctgccac atctggaatg gcaatggttg caggagttct ggatagtatc caaataagag
     8341 aagctgccac caatacaagg ctggccaaca aaatcaggta acgcatctta aatctatgtt
     8401 ttttcggtgc ttgtggttgg taagtttcct ctgtcacagc ctggcttggg tttttgattg
     8461 atttgtgttc tgtctgcgct tgaaccttag gaatagatgt caaggtactc tgagaaacct
     8521 tcggcaaggt cttggtatct gccttgctcg tttcatcaaa gattaactta ctttcatttc
     8581 tacgattgta ggacaagcta ctagacaagt ccacatacat ctctgaaacc gagcggtagc
     8641 gattggtcaa ctttttagca gttgccttga taataacatt ttctaaagcc tgaggtacag
     8701 atggattttc tgcaataacg gacggcaggg gtttctggaa atgctggagg gcaatggtca
     8761 ccgcgctatc cccgtcataa gggatatggc ctgtcaacat ctcatagaaa ataatcccca
     8821 tagcatagat atcactctgc acagtcgcct tcgaaccacg cgcctgctct ggggacaagt
     8881 aatgaactga gcccaacatc gagttagtct gggtcagact tgtctctgca aaggctacag
     8941 caatcccaaa gtctgtgacc ttggcagtcc catctggtgt caaaaggata ttttgaggtt
     9001 tcaagtccct gtgaacaatt cctctggtat gggccaaacg catggctagg aggatttgtc
     9061 ccatgatacg gactgcttct tcattagaaa gaggataatg ttccttgata tagcgtttga
     9121 ggtctagtcc agcaacatac tccattgcaa gatactgttg accgtcttcc tcaccaatat
     9181 ctgttatccg aacgatatga ggatggtcta gatctgccat agctctcgct tcacgctgaa
     9241 aacgagctac agctatcggg tccgtctggt agttggtcct cagaaccttc actgccactt
     9301 cttccccatc taagattaag tctttggcta ggtagacatc cgccatacct cctcgaccaa
     9361 tctgtttgac aatccgatag cgtccggcaa aaatcttgcc gatttggatc attctgcatc
     9421 ctcctcgttc atagaaacaa gggcaaccgt aatgttgtct aaacctcctg cattgttagc
     9481 aaaacgaaca agtgtctccg ttttatctgc taaaggaata tcactggtta caatatcacg
     9541 aatctcactg cctgaaatca tgttggtcaa gccgtcacta ttgagcaaga gatagtcacc
     9601 tgactcaagg ataactgtcc caaaatcagg ctgaatttca tctttttgcc caatagactg
     9661 ggtgataata tttttttgcg gatgagcttc tgcctcttct ggtgtcaatt gaccagcctt
     9721 gagcaactca ttgaccaaag aatgatcact cgtcaactga tggtattctt ctccacgaat
     9781 caagccgata cgcgaatcac caatatgagc atagatagcc tgattatcaa taatagcaag
     9841 gacttccaaa gtagttccca tgcctctgta agcttcatcc tgaccaagct ggtgaatctt
     9901 ttgattttca atttctaggt aatgggcgaa ccattcacgc acttcattga ctgtatcgat
     9961 ctgggtatca acccaagcta cacccaggtc tgtgaccgcc atttcactag cgatattccc
    10021 tgcgcgatga cctcccatcc catcagctaa aataatcatg gtacgtccag ctctattgac
    10081 atagtggttg acatagtctt ggttatttgt tcgtttctga ccaacatctg ttaataatga
    10141 aatttccatg tgtcagttcc ttcctaatcc gatatcttgc gaaattgact gatgaagaat
    10201 ccatcacttc catacaattc aggtgtaatg aggatacagc cgtctttcat gatatcctta
    10261 cattcatgtt ctagttttac ctgctcgaac tcgggatgac tctctaaaaa ggccttaacg
    10321 acttgaaaat tctcctctga gacgatagtg caggtgctat aagttattat accacctttg
    10381 cctagtattt gacaaacact acctaatatt tctaactgaa tttcctgcaa ggacgcgaaa
    10441 tctgccgttt ctttattgta tttgatatct ggttttcggc gcaaaagacc gattcctgaa
    10501 caaggagcat ccaccaaaat cttatcaaag gaatcctggt caaaaaactc atgcaccttt
    10561 ctggcatcca atttttgagt ttgaacccga tctgcaactc ccagacgttg ggcattttct
    10621 tgaattaaat ccaacttgtg gtcgtacaag tccagagcag taacctgacc tgtcgtaaga
    10681 taagaggcta tatgggctgt tttcccacct ggagccgcac aggcatcaag cactcgctca
    10741 tcaccttgta aatcaagcgt cggagcaacc agctgactgg actcgtcttg gatggtaatg
    10801 gctccatccg caaacaaatt atgccctgca aaatgccctt gctccttaac gagaccagtg
    10861 gttgctaaaa gggaattatt cgcctccaac aaggcttgga tttcctcttt tcgacttagg
    10921 tctgttacac gaatactggc tttgtttcgc actaacaggc tttcaaagat ggcttttgct
    10981 ctctcctctc cgtattcttc cttgagtttg gcaactagcc aaactgggag agaataggca
    11041 atggagtcac gcttgttttt tcgcttgatg ctagcaatat ctggccagcc ttcacgcaag
    11101 atacggcgaa ggacagcgtt gaccaatttt tcactgcctt ttttacggag tttggccaat
    11161 tccactgctt cattaaccac agcatgatct ggaatcttgt ccaaatagcg gagttggtag
    11221 gcactcatga gaagaaggac atagagccag ctgtctaact ggtctctgtc ttcgataaag
    11281 tgggataggt accattccag agtcagtcta cgggctaccg ttccatagac cagctcggtc
    11341 actaaggcct tgtctgctgc caaaagttga cttcccttta gatgcttatt taaggcgata
    11401 tttgaatatg cttggttcac aaaaacatcc tctagcactg ctagagctaa acttctagcc
    11461 gtttctactt tagtcaccaa atcgttctcc tacagtcaat gtacgtccaa ctccgttgag
    11521 gaaggaagca atgtccatct taggcttacc agctggctgc acttgtttga gggatagagc
    11581 cccttcagcc gttgcgacaa tcaattcttt cttgccgata gagagaatct cacctggatt
    11641 tccctgacct tctactggta gggcttcata aatcttaaag cggtcgccct taaggaaagt
    11701 atgggcaaca ggccaggggt tcattccacg aatttggtta aagagttgac gattggtttt
    11761 gttccagtcc agtttttctt cctctggctt tatatttgga gagaaggtaa cctgactcgt
    11821 atcctgcggt tcaggtttga tatcaccagc aatataggca ggcagagtgt ccaaaagcaa
    11881 atcacgacca actagcgcca atttttcaaa caaggcgcca acattgtcct catctgtgat
    11941 cggaatgctg cgacgagaaa tcatatctcc tgcatccatt tccttaacca tttccatgat
    12001 ggtcacacca gcttcctcat ccccttgaat caaggcataa tggataggcg caccaccacg
    12061 gtgtctagga aggagggagg catgaacgtt gacagcaaag tccatgctat caaggagttt
    12121 gcttgggaga aactgcccaa aagcagcagt cacaattcca tctgctccta gctttataag
    12181 ttcttccatc tctggacttc cagataattt ttcaggttgg tagatagata gtcctgcttc
    12241 cttggcagcc tgcttgactg gggtttcttg gataactttt ttacgaccaa cagcacggtc
    12301 tggctgggtc acaacggcta gaatttcgta acggtcatct gtcaaaagtc cttttaagac
    12361 tgttgctgaa aagtcggggg tccccataaa gattagtttt gtcatatctt ctccttctta
    12421 taaaaattgc tgcggctcat ggtcaatgct gagacggagc tcactatttt cccgttcttg
    12481 agtcaaggcc aagacctggt tgagggtcag ccccagctca tcttctaaac gatatttaat
    12541 taaaatctgg taatgataga gattgtgggt acgggcaatc ggttttggcg ttggccccag
    12601 aatgggactg gtctctgaca agcctgaccg caaaatgttc atgacttcat aggcacgttt
    12661 gaaaacctct tcttctttct tgtgagaaag ggtaatacca atcgtgaaat agtaaggtgg
    12721 atagcagagt tgtcgtctga ttcccatttc ataggcataa aagccttcgt aatcttgatc
    12781 cttggcaaat cgaatagcat agtgctgcga attgtaggac tgtatcaaga cttgacctgc
    12841 tttttcagcc cgacctgctc gacctgccac ctgagtcaag agctggaagg ttctctcaga
    12901 agaacggaaa tcaggcagat tcaaggccgt atccgcattt agaactccga ctagggtaac
    12961 attgggaaaa tccaaaccct ttgcaatcat ctgagtacca agtaaaatat ccgcttcccc
    13021 tcgcccaaac tggtcaagca aggcttggtg actacctttc tttcgagtcg tatccacatc
    13081 catcctcaga atgcgagctt ggggaaagag ttctgctagc tcatcataag ccttctgagt
    13141 tcccgtccca tagtaacgaa tactgtggct cttacagtta ggacagacct gaggaatatc
    13201 cttcgagaaa ccacaataat ggcagttcat agtcttggta tccatatgca aggtcagaga
    13261 aatatcgcag ttgggacaag tatccaccgt cccacactcc cgacacatga caaagctaga
    13321 ataaccacga cgattgagca tgagaaccac ctgctctttt ttaaccagac ggtcttggat
    13381 agcctctagc aaaggaggcg taaagtttga cgtctcattt tgtccgatat agtctcgaaa
    13441 gtcaatcact tgaacctcag ggattgtagc caaaggattg gcacgttggg ttagacgtaa
    13501 gtgttgatag acgcctttgc cagcacgcgc ccggctttct aagctcggcg ttgcagatcc
    13561 aagtaccaga gttgcttgat tatactgagc ccgtaaaata gctacctctc tggcatggta
    13621 acggggattg ctgtcctgct tataagccac ttcatgctct tcatcaataa tcatgacacc
    13681 cagatttttc agaggagcaa agatagcaga tctggcacca acaacaactt gggcatcgcc
    13741 acgctccacc ttgcgccatt catcatactt ttcaccattg gataatcctg agtgaagaat
    13801 ggctaccttg tccccaaaac gtgctataaa acgctcggtc atctgaggag tcaaggaaat
    13861 ctcaggtacc agcaaaatag ctgtcttgcc cttatccagg gtaccttgga taatctgcaa
    13921 gtaaacctcg gtcttcccac ttcctgtaat cccttgaagt agaaagggag gttgagaact
    13981 gccaatagaa ctcacaaccg catcacgcgc ctgtctttgt tctggattta actccaaagg
    14041 tctacttgct tcaattcctt caaaataagc agccgatcgt tgaacttcct tttggactat
    14101 ggtaacagca ccttgatcca caaagaagtt gacttgctct cgcgagtagg actctaacaa
    14161 gctagccaag gaagcgctct ctggatgaga cagcagataa tctctcagtt ccaacttttt
    14221 cttggcacgt gtagaaatct caacaccttc taattgaaca tggtcaacct cataccaaga
    14281 ctgggtcttg accttctttt gatcgactgc ctgatattcc agaccaagca ggccttttct
    14341 agtcaaacgc atcatttcag cttgcttacc taaatccaga gaagaaaagg caagtgaatc
    14401 ttctgaacca aacaggcgta ctcgttcttc ctgactcaag ccttccagag gatagagaat
    14461 cttgtcatag ctagaattca gaaatcccgg aagcatggcc ttgaggatgg aaattttgta
    14521 ggagaagacg gacttgcgta actcctcagc aagccagagt tgttcttgcg tgagaacggg
    14581 agaaaaatcc agtacctcgg caatatcttt taaatcttgc tccatctctt cttcgtctga
    14641 ttgagactcc aaaccaagaa caatcccttg aatcaggcga ttgcccttac caaaaggcac
    14701 atgaacccgc atcccaactt ccagcattcc ctcaaattcc tccggaatcc tgtaactata
    14761 gggctggtcc gtctgcatca agggcacatc tacgataatc ttagctaggg ccatcttctc
    14821 acctcctcct tgtcagtaca ttcttgcaat agaaaaaata agattgagtc cccccaacct
    14881 taaatttttt caccatcttc tttttcttta gcaatttgct ctttgatttt cttttcttct
    14941 tcttctttgc ggcgtttttc ttcttcgata cggcgacgca ctgcttcacg ttttccttct
    15001 ggatctgggt gaattgtaac gtttcctgat tcgatttctt ctaaagcgcg aagagttgat
    15061 ttttcagact tgaaaccttg agttgctggg gcacctgctt ccaattcgtg ggcacgtttt
    15121 gcttccaaga ttacgagtga atattttgaa gtaaccttgt cgagcaaggt atcaatagag
    15181 ggttttaaca tcatttgctt gtacctattt tctaaatttt atcgggtagt tggagatttt
    15241 ggtaacatct cctgatagtg accaatgaca cgatccacac aaaagtgttc tgcttcaatc
    15301 acacatttga cacgttcagc agctagggat acctgatcgt tgacaatcgc ataatcatac
    15361 tcacgcatga gggcaatttc ttccttggcc ttttcgattc gttgggcaac cacttctgca
    15421 ctatctgttc cacgacctac caagcgttct tgcaattcat ccaaatctgg tggtgtcagg
    15481 aagataaaga cagcatctgg aacctttttc ttgacctgaa gagcaccctg aacttcaatt
    15541 tcaaggaaaa catcgattcc cttgtccaag gtttcattga cataagtcag aggagttcca
    15601 tagtagttgc cgacatattc tgcgtattcc aacatctgtc cttgacgaat cagctcttca
    15661 aattcttcac gagtacggaa gaaatagtcg acaccgtcca cttctccagg acgttgtgcg
    15721 cgtgtcgtca tcgatacaga gtattgaaat tggttttcag aactctcaaa aatctctctt
    15781 ctaaccgttc cttttccaac ccctgaagga ccagaaaaaa cgattagtaa gcctcggtct
    15841 gccattgtgt ctccttttag tcaatctgtg aaataacatt tctctagaat aatggcaaaa
    15901 agccagatta tcctttacag tctttctatc tagtgtaaca aaaaagcagt aatttttcaa
    15961 ctgctctttc ttatttattt agcataatct actgcacgaa gctcgcgaat cacggttacc
    16021 ttgatatttc ctggataatc gagattgttt tcaattttct tacgaacttt gtgagccaag
    16081 attgtgactt tgtcgtcctt gatttttcct ggattgacca tgatacgaat ttcacgtcct
    16141 gcttgaaggg caaagctagt ttgcactcct tcaaagccgt tagcaatttc ttccaaatca
    16201 tggagacgct tgatgtagct ttcaagagac tcactacgag cacctggacg ggctgcgctc
    16261 aaggcatctg ctgcagcgac gataactgct atcacgctct cagcttcaac atctccgtgg
    16321 tgactagcaa tcgtattcac cacaactggg ggttccttgt acttacgggc caattccata
    16381 ccgatttcaa cgtggctacc ttcaacctca tggtcaatgg ctttcccgat atcgtgaagg
    16441 aatccagcac gacgggcaag agccgcattt tcaccaagtt cgctcgccat gataccagcc
    16501 aacttagcaa cctcaatcga atggcgcaaa acattttgtc catatgaagt acggaactgc
    16561 aaacgtccca taatcttcat taagtctgga tgaaggtttg gcgcaccaat ctcataggct
    16621 gctgcctcac cgtattcacg gatcttattg tcaatctctt gacggttttt ctcaaccaac
    16681 tcttcgatac gagctgggtg gatacgacca tccttgagca acatttccat agtcatacgg
    16741 gcaatctcac gacgaatcgg gtcaaatcct gacaaggtca ccacttctgg cgtatcgtcg
    16801 ataatcacat caacacctgt caaactttca aaggtacgaa tgttacgacc ttcacgacca
    16861 ataatgcgtc ccttcatagt atcgtctggt agatgaaccg ttgagtttgt tgactccgct
    16921 acatattcac cagcgatacg ttgcatagct tgaaccaaga tgtccttggc cattttatca
    16981 gaacgttcct tgacctcttg ctcagcttcg cgaatacggc tggcaatctc cttggtcaaa
    17041 ttttcctctg tctgagccaa gataatatct cgtgcttctg cctgagacag ggcaccaata
    17101 cgctctaact ctgcttcttt tcgtctttcg acttcctcta attgctcttc acgcgcatca
    17161 aggtttttcg ctctatcaga aatactttgt tctttttgtt caagtgtttg ttctttactc
    17221 gtcaaattgt cgtccttacg gtcgaggcta gtagctctct cggtcaaacg actttctatt
    17281 tgtttgagtt cttgacgttc tgatttgaat tcagcgtcca cttcttcacg gtattttctg
    17341 gcttcttctt tggcctccaa tagtgcttct tttttaagag acttgctttc acgtttggct
    17401 tcattaacca gtaaatccgc ttcacgttca gcttgtccac gtaaattagt tgcttcttgt
    17461 tcagcattta aaagcatcaa ctccgcagct tcctgagatg atttcatctt agctgagatg
    17521 ctgacatatc caatgactaa accaatgatg acggcaaaaa cagcaatcgc aagcgacatg
    17581 atttccatgt ttttacctca ttttattgtt attctgaatg acatacattc ttttacattc
    17641 taccataaaa aagtgatttt cacaaaccta aaatagaata tgttttgggg aaattagcgt
    17701 gaattttaag attacacttc ttcggatccc ttcaaacttt tgaatacatc atcaataaat
    17761 atatctgcta atctcttctt gaaagaatca ttatttaata gagcaccaaa taaatcttga
    17821 ctctgagagt atccttctat aagagcattg tcaatctctt cttcaaaagc aaaaacgaag
    17881 tcttctctgg agttgacacg agcagattgc tttagtcttg gattcttttt catcaattca
    17941 cgaatggata ccgctccact tagaccgact tggggatcaa tatccaaatc caactgttcg
    18001 ttaatttcat ctaagatttc agataaacgc ttctcctggt cagtcgtaac acctccagta
    18061 cttggcttgg taagtattaa ctctggatga tattccaatt cagaaactga cctgaaggaa
    18121 cctgtttgct tatgtttcat atagtccact acgatcttat ctgctattgt aaaatcatct
    18181 cctccaatgc gaacatcaat catcttgaca agactttgca gataattata acgttcgtgt
    18241 agaacttcat tttgataagc agttgcctga atcaagaaag tatacattct aagaaaacct
    18301 cgaataacct ttcgaataga aagttgttct ttctcatcaa accgtctaac tctatcataa
    18361 ccttgattta acagagaaac catcttttgc ttttctctgc tgtttctctt ttcttggtat
    18421 aagaattgat taaactcatg aataacatca ctgtccaaga taccatactc atcaattttc
    18481 cgatccaagg tcaagacatc actaggtgtg actgtatctg ctaaaatagt ctcacggtag
    18541 tagggagaaa aagctgaacg aatatcgtca taagtatttt taaaatcaag aataaaggtc
    18601 ctcttattgt agcctctata gatacgattc aaacgagaca atgtttgcac cgcagcaaca
    18661 cttcgcaact ttttatctac atacatagca accagtttgg gttggtcaaa ccctgtctga
    18721 tacttatttg ccacaatcag aatttggtag tcttccgtat caaaatgatc tttcaaataa
    18781 tcttccttga cctgattcat ttgcgactca gtatattcac taccttgata atcaaccttg
    18841 ccagaaaagg caatcagggt tccaatactg gtaatgctgt ggtctttgaa gtacttttga
    18901 aaggctaatt gatacttaac tgctgcctca cgaccagagg tcacaatcat agccttacct
    18961 tgaccaccca attcagccat aatatcatga ctgataaagt gatccatgat aatttcaacc
    19021 ttttgattga tattttcatc agataattct acaaactttg caatcttacg cttggcaaga
    19081 gtcgttttta gctcaggatc ttcttcgact gctttattga gcttgtaata ggtcttataa
    19141 gtcgtatagt tatctaaaac atctagaata aaaccttcct caatagcttg cttcatacta
    19201 taaacatcaa aaggagcttt agaaccatct gcttgtgttg taccaaatag ctgtagagtt
    19261 gtcgccttgg gagttgcagt gaaggcaatg attgagacat tgtcctgctt acctgtccga
    19321 gcaatctcct gctcgatagc gtccgcaaca cttacttctt gaatctcatc gatagttcca
    19381 tcttctgtat ctgtttctgt caagacttga ctaaccgaac tcatatagga tcctgtcgtt
    19441 gaactgtgag cttcatcaat caggatggca aatttctttt tcgcagtgtt ccctacagac
    19501 atccagttag attggttgat ttgggcaaac ttatgaatcg tcgtagctat gatttttgta
    19561 tttcctgcaa tcgcatccgc taagtcactg gaggtttctt tgtcgcccat taccttgacc
    19621 actccaggct tgtgggaaat ttccttgacc gcttcttgta actgacgatc aactacgata
    19681 cggtctgtaa cgattaatac agtatccaca atattttgat tttgttcatc atggagcgat
    19741 accaaactat gagccaacca tgctatggta ttggttttac ctgaaccagc tgagtgctga
    19801 atcagataat tactgtctgt atggttgact ttcatgtctt ctaggagacg gctaactgct
    19861 cttcgctgat ggtatctcgg gaaaatcagg gtcttctttt tagaattatg cttatccatc
    19921 tcatccatct caaaaaagat aaaactatct attaactgga gaatactatc cttggtcaag
    19981 acatcaagcc acaagtagtc tgtatcgaca ccatcagggt tgtgagggtt gccacttccc
    20041 atctcaatac cttccccttt ccccacatta aaaggtagaa agtatgtttc ttgtcctttc
    20101 aattgcgtcg tcatatagac ttctagggta tccactgcaa aggaagccaa gactccctgt
    20161 tcaaaacgga aaagacgatt cttaggattg cgtcccttga gctgcctgat agcatcctga
    20221 acagactgac cactgggatt aaacttaagt tcgatagcaa atagtgctaa tccattgata
    20281 aagataacaa ggtcaatgcg gtcgctctcc tcgtaaacca cttcttccat aatagataga
    20341 cagttgactt ggtattgttg aacagccttt tcattaaaac tagtcgcagg tttacgataa
    20401 agaagacgaa gttgtttatt ttcaataaaa actccttctt tcaaggcgaa taaaaagcca
    20461 cccttgacga tttctttatt aatcagattg acgattgtct ccttactcag ttttttaagg
    20521 agatagtcca tggtctcctc ttgggtctcc atcagaaaat cccacaggag ttccacatcc
    20581 atggctaatc taggatcgta gcaagtagcg tcccgctgaa tatagtcatt cttatctctc
    20641 aaatctttga taatccactg ctggaaatca tagcgctcct tcaagcccaa atttgccata
    20701 gcttctcact ttctttttct ataattcaat taaaaatttc
//
LOCUS       DAVKDZ010000032        20192 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000032 DAVKDZ010000000
VERSION     DAVKDZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20192
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.033"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(81..824)
                     /gene="srtA"
                     /locus_tag="VWZ30_001158"
     CDS             complement(81..824)
                     /gene="srtA"
                     /locus_tag="VWZ30_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEU9334912.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
     gene            complement(824..3292)
                     /gene="gyrA"
                     /locus_tag="VWZ30_001159"
     CDS             complement(824..3292)
                     /gene="gyrA"
                     /locus_tag="VWZ30_001159"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU9334913.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDVVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            3486..4472
                     /locus_tag="VWZ30_001160"
     CDS             3486..4472
                     /locus_tag="VWZ30_001160"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEU9334914.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            complement(4728..5504)
                     /locus_tag="VWZ30_001161"
     CDS             complement(4728..5504)
                     /locus_tag="VWZ30_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEU9334915.1"
                     /translation="MNKKWLFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENIFDLLTILPLNAIFTVFRLGRIFRLARLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPVSLFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSSQRILIEKQSEKIEELHRMIKDLVEKIS"
     gene            complement(5551..5805)
                     /locus_tag="VWZ30_001162"
     CDS             complement(5551..5805)
                     /locus_tag="VWZ30_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU9334916.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            complement(5807..6049)
                     /locus_tag="VWZ30_001163"
     CDS             complement(5807..6049)
                     /locus_tag="VWZ30_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU9334917.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            complement(6140..6949)
                     /locus_tag="VWZ30_001164"
     CDS             complement(6140..6949)
                     /locus_tag="VWZ30_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9334918.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            complement(6951..8300)
                     /gene="vicK"
                     /locus_tag="VWZ30_001165"
     CDS             complement(6951..8300)
                     /gene="vicK"
                     /locus_tag="VWZ30_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEU9334919.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            complement(8293..8997)
                     /locus_tag="VWZ30_001166"
     CDS             complement(8293..8997)
                     /locus_tag="VWZ30_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9334920.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            complement(9053..10228)
                     /gene="mutY"
                     /locus_tag="VWZ30_001167"
     CDS             complement(9053..10228)
                     /gene="mutY"
                     /locus_tag="VWZ30_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU9334921.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            complement(10556..12226)
                     /locus_tag="VWZ30_001168"
     CDS             complement(10556..12226)
                     /locus_tag="VWZ30_001168"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU9334922.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRAVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVTKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            12457..13146
                     /gene="coaB"
                     /locus_tag="VWZ30_001169"
     CDS             12457..13146
                     /gene="coaB"
                     /locus_tag="VWZ30_001169"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEU9334923.1"
                     /translation="MKILVTLGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEIQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            13158..13709
                     /gene="coaC"
                     /locus_tag="VWZ30_001170"
     CDS             13158..13709
                     /gene="coaC"
                     /locus_tag="VWZ30_001170"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU9334924.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            13693..14256
                     /locus_tag="VWZ30_001171"
     CDS             13693..14256
                     /locus_tag="VWZ30_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9334925.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            14538..15065
                     /locus_tag="VWZ30_001172"
     CDS             14538..15065
                     /locus_tag="VWZ30_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9334926.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            15077..15592
                     /locus_tag="VWZ30_001173"
     CDS             15077..15592
                     /locus_tag="VWZ30_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEU9334927.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALEQLGYLVKD"
     gene            complement(15589..16056)
                     /locus_tag="VWZ30_001174"
     CDS             complement(15589..16056)
                     /locus_tag="VWZ30_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9334928.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRSPDTNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLRS
                     SEEGEVSWVQKDQIPNLDLAYDMLPLMEMMEAPDKSEFFYRHRTEDDWEKKIF"
     gene            complement(16109..16618)
                     /locus_tag="VWZ30_001175"
     CDS             complement(16109..16618)
                     /locus_tag="VWZ30_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9334929.1"
                     /translation="MTVIIKSMETPKEIEGKSLVHWQMWREAYDDLLPAEFQETMTLE
                     RCRLFSQKYPENTLIAIGDLKVVGFISYGNFRDETMQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SKIILKSRS"
     gene            complement(16584..17147)
                     /locus_tag="VWZ30_001176"
     CDS             complement(16584..17147)
                     /locus_tag="VWZ30_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9334930.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            complement(17157..19145)
                     /gene="uvrB"
                     /locus_tag="VWZ30_001177"
     CDS             complement(17157..19145)
                     /gene="uvrB"
                     /locus_tag="VWZ30_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU9334931.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            complement(19222..20175)
                     /locus_tag="VWZ30_001178"
     CDS             complement(19222..20175)
                     /locus_tag="VWZ30_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9334932.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAYTLYGIS
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENCLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLTILISSVLFTLLHVGNAGLTPLS
                     LVNLFLFGVAMALYLLKIDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
BASE COUNT         6001 a         4518 c         3526 g         6147 t
ORIGIN      
        1 gtcctattct tatttcattt tactatattt ttatgaaaat cttaaaaaat cagtgaaatc
       61 catgatttca ctgatttgta ttaataaaat tgtttatatg gttgattgaa agctgttagg
      121 atttcatcag atgtttgtga ataatctttt gtttctttca aatcaccttt gacaataata
      181 cgttctgtag cagcaaggtc ttcacaggtt actaatgtga tttcattgac cccatctcta
      241 tcatcaactt catcaacacg atccggtgtc acacgtttga cttcacgtat ttcataagta
      301 taaactttat ttttatcggt tagataaatc ttcatgccat ttttagcatt atctaaagga
      361 gaaaataaca ttttattagc attatcaaca ccaaagatat ggtgactagc tagactataa
      421 tttccttctc ccattacttg ctcgcgtttc attgtaccag ctccgtagaa gagattaaca
      481 ttatcaagtc ctttaaaaat cggcaaattc atttccaatt caggaattgc aattccccca
      541 ataactggta atttttgagc atcccattga gaagttagaa cagcttccga agagatagct
      601 ttgacagaat caaagtcaaa attgccttct gtatcctgat tttcttctaa tttctctttt
      661 gatacctggc taacttgata cttattggta ttccagacta tgaaaatatt tcgaatttga
      721 gtattaaaaa tcaaagccag tgacagtaat atcagaaatc ctgctaggat atttgtcagc
      781 agattttttc gcttgttttt ctttttatta tttttttgag acattatgct tcaccttctg
      841 tttcattttc tgtcccaact tcttcttttt ctgccaccgc aaccgttgtg aaagtcacta
      901 tctgagcatc ttgatccagg cgcattactt taactcccat agttgcacgt cctgtttgtg
      961 aaatattggc aagattggtt cgaatcatga cacctgtatc agtgataatc atcaaatcct
     1021 catccccttg aacagtcata agaccggcca gcaagccatt tttttcggta attttagctg
     1081 tctgcattcc cttaccacca cgaccttttg ttgggtattc agtagcgact gtacgcttac
     1141 catatccttt ttctgtgata ataagaacct catcttgatc agtaatcaag ctggcaccaa
     1201 caactgtgtc tccttcacga aggttaacac ctttcacacc agtggcgata cgactcatac
     1261 cacgaacggc tgattgatta aagcgaactg cataaccaaa cttggtacca atgataatat
     1321 ccatatctcc ttctgccaac aagacattga ttaactcatc ttcatccttt aaattcagcg
     1381 ctttgagacc attttgacga atattggcaa actccttaac actggttctc ttcacaatac
     1441 cgtgacgggt tgtaaagaag agataagcat catcactgcg atcagactca acattgataa
     1501 ccgtctgaat actttcgtct tcatccaatt tcaagagatt gactactggt agccctttgg
     1561 cagtccgacc atactcagga atttcataac ctttaagacg atagacacgt cccttgtttg
     1621 tgaagaagag cagatgatca tgggtgctag ttgacactaa ctcacgaaca aagtcatcat
     1681 ctttcactcc cgttccttgg acaccacgac ccccacgttt ttgagcagtg aactcgtcct
     1741 gatccaaacg cttaatgtag cctctgttag aaagggtaat caagacatcc gattcttcaa
     1801 tcaagtcctc atcctcgaga ctcaagatct gtccaaccat caactctgta cggcgtttat
     1861 cagaaaattt acgtttaact tcatccaatt cgtctttgat aatttgagaa acacgttcag
     1921 gcttagcaag aatatctgct aaatccgcaa tcagagccaa gaggtcatca tactcagatt
     1981 gaatcttatc gcgttccaaa cctgtcaaac gacgaagacg catatcaagg atagcttgac
     2041 tttgacgttc agaaagctta aacttgctca tcaactcagc ttgagcttcc gcatccgttt
     2101 cactagcacg gatgatacga atcacttcgt cgatatggtc tagcgcaatc aagagacctt
     2161 ctaagatatg agcgcgcgct tccgcttttt ccttatcaaa acgtgtacga cgaacaacca
     2221 cttctttttg gtgctcgata taagcatcca aaatctgacg aagagacaaa attttcggta
     2281 taccattttg gatagcgagc atattgaaac caaaattggt ttgcatttgg gtcattttga
     2341 agaggttatt gagaataaca ttggctgagg cgtcgcgctt gacttcaata acaaatcgaa
     2401 caccttcacg gtttgactca tcacgtactg ctgtgatacc ctcaatgcgt ttttcctgaa
     2461 ccaagcgaac aatatgctca tgcaccttgg ttttattgac catgtaagga aattctgtta
     2521 caacgatgcg ctcacgacca gtcttagtcg tttcaatctc tgtacgagaa cgtaggacaa
     2581 tcgaaccttt acctgtttca taagccttat ggatacctga tttccccatg acaagagcac
     2641 cagttggaaa atctggtcca ggcaagactt ccatcaagtc cttggtagtc acttcaggat
     2701 tatccatgac caacttcact acatcaatgg tttcacccag attatgaggt ggaatattgg
     2761 ttgccatccc aaccgcaata ccagttgctc cattaaccaa aaggtttgga aaacgcgctg
     2821 gcaagaccaa gggttcccgt tcattggcat catagttatc aacgaaatca actgtatttt
     2881 tgttgatatc acgaagcatt tccagagcaa tcttgctcat acgtgcctcg gtataacgtt
     2941 gagcggcagc actatctcca tccatagaac caaaattccc atgaccatct acaagcatgt
     3001 aacggtagct ccaccattga gccatacgga ccatggcttc ataaatagag gaatccccgt
     3061 gtgggtgata tttacccatg acatcccctg taatacgagc agatttttta tggggtttgt
     3121 ctggggtcac acccaattca ttcattccgt agagaatgcg acggtgaaca ggttttaagc
     3181 catctcgaac atcaggaaga gctcgcgcta cgataacact catggcgtag tcgataaaac
     3241 ttgccttcat ctcctttgtc agattgacat tcactaaatt tttatcctgc attaataaat
     3301 gcctcatttc acaattagta agtaacaaca ttataccata aattcccatc tatttcagcc
     3361 tctaaaccac taaaacgttt acatcgagaa ctataaggca tattcgtgac aaagtttttt
     3421 aaaagtgata gaatgaagtt gtctagggaa aatcccctaa taaaattaag gagatgttta
     3481 aaacaatgac ttcaactaaa caacacaaaa aagttatcct tgtcggtgat ggtgctgtag
     3541 gttcatctta cgcttttgca cttgttaacc aaggaattgc acaagagctt ggaattatcg
     3601 aaattccaca attgcatgaa aaagctgttg gtgatgcgct tgaccttagt cacgcccttg
     3661 ccttcacttc acctaaaaaa atctatgcag ctcaatactc tgactgtgca gacgctgacc
     3721 ttgttgtgat cactgcaggt gcacctcaaa aaccaggtga aactcgtctt gaccttgtag
     3781 gtaaaaacct tgctatcaac aaatcaatcg taactcaagt tgttgaatct ggtttcaaag
     3841 gtatcttcct tgttgctgct aacccagttg acgttttgac ttactcaact tggaaattct
     3901 ctggtttccc taaagaacgc gttatcggtt caggtacttc acttgactca gctcgtttcc
     3961 gtcaagcact tgctgaaaaa ttggatgtgg atgctcgttc agtgcacgcc tacatcatgg
     4021 gtgaacacgg tgactctgag ttcgctgttt ggtcacacgc taacatcgct ggtgtaaacc
     4081 ttgaagaatt ccttaaagac actcaaaatg ttcaagaagc tgaattgatt gaattgttcg
     4141 aaggtgttcg tgatgcagcc tacacaatca tcaacaaaaa aggtgcaaca tactacggta
     4201 tcgcagtagc ccttgctcgt atcactaaag caatccttga cgatgaaaac gcagtacttc
     4261 cactttcagt attccaagaa ggtcaatacg gagttgagaa tgtctttatc ggtcaaccag
     4321 ctgttgttgg tgcacatggt atcgttcgtc cagtaaacat tccattgaac gatgcagaaa
     4381 ctcaaaaaat gcaagcatct gctaaagaat tgcaagctat tattgacgaa gcatggaaaa
     4441 atccagaatt ccaagaagct tctaaaaact aatttaacta tagacaactc cttgaatcat
     4501 cagggagttg tttctttctg tattatagta ggttgaagtt gaaatagcac cccattactc
     4561 taaaacgttg ttataaaatg ctttaaattc ctaattgatt tgttcaaatt ttatttcatt
     4621 tctctataaa acgcatatta tcaaattttt atacctgata atatgcgttc tattgtgtca
     4681 aaacttactt gatttctcct cgaatcaagt tttgttttgt ctcaccttta tgatattttt
     4741 tcaactaagt cttttatcat tcgatgtagt tcttcgattt tttcagattg tttctctatt
     4801 aatattcttt ggctcgataa ttcgtctttg agtttatcaa gttttatctg ttcatctgga
     4861 ttatccttta caaaaaagtt cgtcaaagca cttgttagca tccctattgt tccaataccg
     4921 acaagcatca gtaaaactgc tagccatttc ccaaaaaggg aaactggaac aatatcacca
     4981 taaccaacag tagttactgt cacaagagcc caccaaagac tatctgagaa ggatttttct
     5041 tcaacaacgg ataaaatcga actccctact agaacaataa agatattaac atataagata
     5101 taaatcaggc catttgtccg taagaatcta ctaatttttc tttctaattt acctgtaagt
     5161 ccaattattc taagtaaacg agtcagtttc agtaattttg tcagccttgc taagcggaag
     5221 atacgtccta atcgaaacac tgtaaaaata gcatttaaag ggagaatagt caataaatca
     5281 aaaatatttt caagaataaa gcgccatttt cttttagtaa taaaaaagcg ccaactataa
     5341 tcaattacaa aaacacccca gattactaaa tcaataatac tataaggcgg attgtccaaa
     5401 tcaatcatct cagcaaatcc aagtaatacc agaataacag agattaaagc aagtaaaata
     5461 attgttgtat cataataatc tgcaaataac cattttttat tcaaattttt actatcctta
     5521 ctttctatac tgttattact cagctttaat tcaataaata tctctccgat gaccaacttc
     5581 tagagtagca acgactaatt catcatctac aatttgtacg ataactcgat aattaccaat
     5641 tctatagcgc cattgaccaa cgcgattacc aaccaaagcc tttccgtgtc gtcttgggtc
     5701 ttccaaaaca ttggtttgta aatagtttgt aattagcttc tgcgtataac ggtccaattt
     5761 tttcaattgc ttgataaaac gtcttgttgg aactaattta tacaaattat tcatccttca
     5821 agcctaaatc atgcatcatt tcttcccaag taatgggttc aactcctttt tccaagtctt
     5881 ctaaatactc ttgataggct aaatctgcca cacgagcatc gtattcatct tctagggctt
     5941 caagagtttt ggtgcgaata agttccgaaa gggaaactcc ttcaaactta gccattgctt
     6001 tcataaatgt tttatcagct tcagaaactt ttaatgtaat agtagtcatc ttttgtgctc
     6061 ccttttttaa tggtaacacc attgtattac tttttatgtg ttcagtcaat ataaaaagaa
     6121 caccttctca gcgttctttt tatatctctg tcaatggtgt tgcggtatct ggtgaggtat
     6181 cataaacctt aaagtctact ccgactccca gatcagcttg agccagctga ttgaccatgg
     6241 tcatatgagc cagttccttg atattatttt ccttagataa atgcccaagg taaatcttct
     6301 tagtacgatt tcctagcgtc cgaatcatag cttcagcacc gtcctcgtta gaaaggtgac
     6361 caaggtcaga taggattcgt tgtttgagtc gccaagcgta agaacctgat cgcaaaatct
     6421 ctacatcatg gttggcctcg ataagatagc catccgcatt ttcgacaatg cccgccatac
     6481 ggtcactgac ataacctgta tctgtcaaaa ggacaaaact cttatcatcc ttcataaagc
     6541 gatagaactg cggtgcgact gcatcatggc ttacaccaaa actctcgatg tcgatatctc
     6601 caaaggtttt ggttttaccc atttcaaaaa tatgcttttg cgaagaatcc accttgccaa
     6661 gatatttact attttccata gcttgccagg tcttttcatt ggcataaaga tccataccat
     6721 acttgcgagc caaaacgcct actccatgga tatgatctga atgctcatgg gtaatcaaga
     6781 tggcatccag gtcttctggc ttacggttaa tttcagctag cagactggta attttcttgc
     6841 cagacaagcc tgcatctact aaaagcttct tttttgaggt ttccagataa aaagaatttc
     6901 cactggaacc cgacgctaaa atactgtatt taaagcctat ttcactcatt ctagtcttct
     6961 acttcatcct cccatacttc ttctttcact gcatccttat cataagggag cacaatggta
     7021 aaggttgaac ccttgccgta ttcactcttg gcccaaataa agcccttatg ttgtttgata
     7081 atttctttag cgatagacag tcctagacct gtaccacctt gtgcacgact tctagcacga
     7141 tccacacgat agaaacggtc aaagatacgt ggtaaatcct gcttaggaat ccccaaaccg
     7201 tggtcagaaa tggataaaat catctggtct tcagttgtct tcattctgac agtgatttta
     7261 cccccatctg gcgaatactt aatagcatta tttaaaatat tgtcgacaac ctgcgtcatc
     7321 ttatctgtat caatttccat ccagatagaa ttgatgggat aatctctcac caactcatat
     7381 tttttctcct tttcctgtcc tttcatcttg tcaaaacgat tgaggataaa ggtaataaaa
     7441 gcagtgaagt taatcagttc cacatctagg tgactggtag cattatcaat acgtgaaaga
     7501 tggaggagat ccgtcaccat gcgcatcata cggttggtct catcaagaga aaccttgata
     7561 aagtctggtg ctacagtttc acacaaagcc ccctcatcca aggcttcaag ataggatttt
     7621 acgctagtca gaggagtccg taactcatgg ctaacattgg aaacaaagag tcttcgttcg
     7681 cgttcttcct tctcctgctc cgtcgtatca tgcaaaacag ccaccaaacc tgaaataaag
     7741 ccagactctc gacgtatcaa ggcaaagcga actcgaaggt tcaaatattc accattgata
     7801 tcttgggaat ctagcaacaa ttctggactt tgggtaatca aatcacgcaa ttcatagttt
     7861 tcttctatct tgagcaattc caaaatgctt ctattcagaa catcttcctt aaccaacccc
     7921 agttgcttct tggctgtatc gttaatcatg ataatctgac cccgacggtt ggtcgcaaga
     7981 accccatctg tcatataaaa cagaatacta tttagcctct tactctcttg ttctagattt
     8041 tcctgagtga gacgaataac ctccgacaag tcattcaaat tattggtaat attggtgatt
     8101 tcagacccac cttgcatatc aagaaccttg gaataatctc ctgcaatcaa atctttaacc
     8161 ttttgattga tttgcttcaa ctgaatatta tcacgtctat tttccagtaa taagagggtc
     8221 acaacaagga tgaaacctaa caaaatcagg ataaagataa aatctctggt aaaaatggtt
     8281 tgtttcagta aatcaagcat tatttctcat gtaataccct acaccacggc gtgtcaagat
     8341 atactctggt cggctgggcg tatcttcaat cttctcacgc agacgtcgta cagtcacatc
     8401 aactgtacgg acatcaccaa aatagtcata accccagaca gtctcaagca agtgttcgcg
     8461 cgtgatgact tgacctgtat gcgatgctaa atgatacaaa agctcaaatt cacgatgggt
     8521 taagtctagt tcttcgccat attttttagc cacgtaggcg tctggaacaa tttctaaatc
     8581 cccaatttgg ataggttgag gtttactatc tgcttcctga ccatctactg gcataggttg
     8641 agaacgacgc agaagagctt taacacgcgc ctgcaactca cgattggaga agggtttcgt
     8701 tacatagtca tctgccccaa gttccaaacc gataacctta tcaaattcac tatctttggc
     8761 tgaaagcata agaatgggca cactgcttgt cttacgaatg gtcttagcaa cttctaaacc
     8821 atcaatttct ggaagcatca aatccagaat aataatatct ggttgctctg cttcaaattg
     8881 ctctagcgct tcacgaccat taaaagcagt tacaacttcg taaccttcct tggtcatatt
     8941 aaacttgata atatccgaga ttggtttctc atcatctaca attagtattt ttttcatatg
     9001 ttcacctttt tctctactat tataccaaaa aaatagtcag aagacacaat agctagtctt
     9061 ggctactgtc taagttggct tgtgcataag cctgccagat tttttgttgg ggtttggcaa
     9121 gtgggtaatt cttgaattct tctggtgaaa gccagcgaac ttccctatct gaaaaatcat
     9181 ggaagtcact cacctgacct gctacaatct gtacatgcca ttttcgatga ctaaaaacat
     9241 gctggactgt atcaaaacaa acatcaagcc aatcaacatc taggtcatag tcctgctgga
     9301 aactctcttc tggactggga ccaaagttca cactttcttc cgcaacctga tgaaagaggt
     9361 caaactgctc ttcttgcgaa aagttatcaa cttctataaa ggggaaatgc caaaaacctg
     9421 ccaagagctt ttcgctttca tttttttcaa gtaaaaattg tccttgagaa tttttcacaa
     9481 ctaaggcttt aagataaata ggaaccggct ttttcttagg agatttaatt ggataacggt
     9541 ccatggttcc attctgatat gccgcactaa agtccttgac tgggctttct tcaggtctgg
     9601 gatttacagg agactcaata tcagacccta agtccatcaa ggcttgatta aaatcacccg
     9661 gacgatccgg attaatcaag atctccatca ttgcctgaaa aatttttcga ttacttggaa
     9721 tcccaatatc gtggttgact tcaaacagac gcgccaagac ccgcatgaca ttaccatcta
     9781 cagctggctc aggcaagtta aaagcaatac tggaaatggc tcctgctgtg taaggtccaa
     9841 tccctttcaa gctggaaatt ccttcgtagg tatttggaaa ttggccacca aagtcagtca
     9901 taatctgctg ggctgcagcc tgcatattgc gaactcgaga ataatagccc aagccctccc
     9961 aagctttcag taaactctcc tcaggcgcag ttgccagact ttcgacagtt ggaaaccagt
    10021 ccaggaatct ttcgtagtaa gggataactg tatccaccct ggtctgctga agcatgattt
    10081 cagataccca gatgtgataa ggatttttac ttctcctcca aggcaaatct cttttgtttt
    10141 catcatacca agcgagaagt ttctcacgga aagaaatgac tttctcctcc ggccacatga
    10201 cgataccgta ttctttcaaa tctaacatat ctctagtata acacagaagg tttcacctgt
    10261 ctttgtatct gatttataat attttcaata gatagtatat aacttttcta tctacttata
    10321 ctcaatgaaa atcaaagagc aaattaggaa gctagccgca ggttgctcaa aacactgttt
    10381 taaggttgtg gatagaactg acagagtcag tatcatatac ctacggcaag gtgaagctga
    10441 catagtttga agagattttc gaagagtata aatcttattg atgaactgct tgcagtctga
    10501 gaaaaaatga gcttggatat tatttccaaa ctcacttaaa gtcaatttca atccactaga
    10561 acaagcctag tacagttcca tcgctttcaa catccatgtt gagagctgct ggacgttttg
    10621 gaagacctgg catggtcata acatcaccag ttaaggcaac gatgaagcct gcacctaatt
    10681 ttggtaccaa ttcacgaatg gtaatttcaa agttttctgg tgctccaagc gcatttggat
    10741 tgtctgagaa actgtattga gttttagcca tacagattgg caatttgtcc caaccgtttt
    10801 gaacgatttg agcaatttgt gtttgagctt tcttctcaaa gttcactttg ctaccacgat
    10861 agatttcagt gacaattttt tcaatctttt cttggacaga aaggtcatta tcatacaaac
    10921 gtttatagtt agctggattt tcagcaattg tcttaacaac tgtttcggca agtgctactc
    10981 caccttctgc tccatcagcc cagacactag ccaattcaac tggtacatcg attgaggcac
    11041 agagttcttt taagactgca atttcagctt ctgtatcaga tacaaattcg ttaatagcta
    11101 caactgctgg aataccgaac ttacggatat tttcaacgtg gcgtttcaag ttagcaaaac
    11161 ctgcacgaac tgcctctaca ttttcttcag tcagagcgtc tttagtcaca ccaccattca
    11221 tcttaagggc acgaagggtt gcgacaataa caactgcatc tggagatgtt ggcaagtttg
    11281 gtgtcttgat atcaaggaat ttctcagcac caaggtccgc accaaaacca gcttcagtaa
    11341 cagtgtaatc agccaagtga agggctgttg tcgtcgccaa aacagagtta catccatgag
    11401 cgatattggc aaatggacca ccgtgtacaa aggcaggtgt accgtaaatt gtctgaacca
    11461 agtttggctt aatagcatcc ttcaaaatca aagccaaggc accctcaacc tgcaaatcac
    11521 ctacagaaac aggcgtacgg tcatagcgat aaccaataac gatattcgcc aaacgacgtt
    11581 tcaagtcctc gatgtccgtt gccaagcaaa gaattgccat gatttctgaa gcaactgtaa
    11641 tatcaaaacc atcctcacgt ggaataccgt ttagaggacc accaagacca acagtcacat
    11701 ggcggagcgc acggtcgttc aagtccacag cgcgtttcca gaggatacga cgttgatcaa
    11761 ttcccagctc attcccttgg tgcaagtggt tgtcaatcaa ggcagaaagg gcattgttgg
    11821 cagttgtaat agcatgcata tctccagtaa agtggaggtt gatgtcttcc attggcagaa
    11881 cttgtgcata cccaccgcca gcagcaccac ccttgatccc catgactgga ccaagagacg
    11941 gttcgcggat agcaatcatg gttttcttgc caatcttgtt caaggcatcc gcaagaccaa
    12001 tggtaagcgt cgactttcct tcacctgcag gtgttgggtt gatggcagta accaagatca
    12061 atttaccgac tggattgctc tcaactgcac gaattttatc aaagctgagt ttagccttgt
    12121 actttccgta caactccaaa tcgtcgtaag aaataccaag tttctctaca acatcaacaa
    12181 ttggcttcaa ctcaatactc tgtgcgattt caatatctgt tttcattcaa aattcctcta
    12241 acctcttata tgataattca ttatatcaca aaacaagatt tttaacaccc taaaactctc
    12301 taaacgttcg taaatatctc tgttttttaa ggcttttaga gtcctttctt aaattttata
    12361 tggctttata gtttgaaact atagtaaatc ttcgttttta ccaaaaattt atcactttca
    12421 ttttacttac cgcttatttt tgtgtacaat agtgctatga aaattttagt tacattgggc
    12481 ggtaccagtg aagctatcga tagcgtccgc tctatcacta accattctac aggtcacttg
    12541 gggaaaatta tcacagagac tttgctttct gcagggcatg aagtttgttt aattacgaca
    12601 aaacgagctc tgaagccaga gcctcatcct aacctaagta ttcgagaaat taccaatacc
    12661 aaggaccttc taatagaaat acaagaacgt gttcaggatt atcaggtctt gatccactca
    12721 atggctgttt ctgactacac tcctgtttat atgacagggc ttgaggaagt tcaggctagc
    12781 tccaatctaa aagaattttt aagcaagcag aatcatcagg ccaagatttc ttcaactgat
    12841 gaggttcagg ttttgttcct taaaaagaca cccaaaatca tatccctagt caaggaatgg
    12901 aatcctacta ttcatctgat tggtttcaaa ctgctggttg atgttaccga agatcatctg
    12961 gttgacattg cacgaaaaag tcttatcaag aatcaagcag atttaatcat cgcgaatgac
    13021 ctgactcaaa tttcagcaga tcagcaccga gctatatttg ttgagaaaaa tcagcttcaa
    13081 acagtccaga ctaaagaaga aattgcagaa ctcctccttg aaaaaattca agcctatcat
    13141 tcttagaaag gaaaactatg gcaaacattc tcttggctgt aacgggttca atcgcctctt
    13201 ataagtcggc agatttagtc agttctctaa aaaaacaagg ccatcaagtc actgtcttaa
    13261 tgactcaggc tgctacagag tttatccaac ctttgacact acaggtactc tcacagaatc
    13321 ctgtccactt ggatgtcatg aaagaaccct atcctgatca ggtcaatcat atcgaacttg
    13381 gaaaaaaagc agatttattt atcgtggtac ctgcaactgc taacactatt gcaaaactag
    13441 ctcacggatt tgcggacaac atggtaacca gtacagctct agccctacca agtcatattc
    13501 ccaaactaat agctcctgct atgaatacaa aaatgtatga ccatccagta actcagaata
    13561 atctgaaaac attagaaacc tacggctatc agctgattgc tcctaaggaa tccctactag
    13621 cttgtggaga ccacggacga ggagctttag ctgacctcac aattatttta gaaagaataa
    13681 aggaaactat cgatgaaaaa acgctctaat attgcaccca ttgctatctt ttttgctacc
    13741 atgctcgtga tacactttct gagctcactt atctttaacc tttttccatt tccaatcaaa
    13801 ccgaccattg ttcatattcc tgtcattatt gccagcatta tttatggtcc acgagttggg
    13861 gttacacttg gatttttgat gggattactt agcttgacgg ttaacacgat tacgattcta
    13921 ccgacaagct acctcttctc tcccttcgta ccaaacggaa acatctactc agctatcatt
    13981 gccattgtcc cacgtatttt gattggttta actccttact tagtctataa actgatgaaa
    14041 aacaagactg gtctgatttt agctggggct cttggttcct tgacaaatac tatctttgtc
    14101 cttggaggaa tcttcttcct atttggaaat gtttataacg gaaatatcca acttcttctg
    14161 gcaaccgtta tctcaacaaa ttcaattgct gaattggtca tttctgcaat tctaacccta
    14221 gccattgttc cacgactaca aaccttgaaa aaataaaaac aatctccgct ttcaaacctg
    14281 aaggtggaga ttttgctcta tacagtttct ttttaatgaa atctttacta tagtaaaacg
    14341 aaataagaac aggacaaatt gatcaggaca gccaaatcga tttctaacaa tgttttagaa
    14401 gtagaggtgg actattctag tttcaatcta ctataacttt taactaaaaa atgatctata
    14461 aacccaagta aattcttgct ttattgtctg gtttattgta ccatactagt gtatatgcag
    14521 ttaaaaagga gattcttgtg aatacacgga aaaagacaca atttatgaca atgacagccc
    14581 ttttaacggc tattgcgatt ttgattccaa ttgttatgcc tttcaagatt gtcattccac
    14641 ctgcttccta tactttgggg agccacatcg ctatttttat agccatgttc ttgtcgccct
    14701 tgatggcagt ttttgtcatc ctagcctcta gttttggatt tttgatggct ggctatccca
    14761 tggttatcgt ttttcgggct ttttcccata tatcttttgg tgctttagga gctctttacc
    14821 tacaaaaatt ccccgatacc ctagataaac caaaatcttc ctgggttttc aactttgttt
    14881 tggctgttgt tcatgccctt gctgaagtat tagcctgtgt ccttttttac gcaacttcgg
    14941 gtaccaatgt agaaaacatg ttctatgttc tatttgtgct agttggattt ggcacaatta
    15001 tccatagtat ggtagactat acattagcac tagctgtcta taaagtgctt cgaaaacgcc
    15061 gttaaaagga aaagctatga caaaagatcg caaacaagcc ctgctccaac tcttgaaaga
    15121 agctcctaaa gcccttaatg gccaaagttt ggcggaacat tttcatgtca cacgtcaggt
    15181 cattgtgcag gacattgcaa ttttgagagc cgatggcgct cctatcctat ccaccaatcg
    15241 tggctacatc tataagcaaa ttgataccaa tccttatgtt cacaaacttt tcaaagtgaa
    15301 acatgaagtt gaagaaatcg gtcaggaact ccttgctatt gtcgataatg gtgggcatgt
    15361 ccagaatacc ttgattgacc atcccgttta tggagaaatt gaaaccttgc ttaaactgtc
    15421 ttgtcgcaga gatgtgcaac attttctaga acaagtcgag cattctgact ttagacctct
    15481 atctgaattg acagatggca tccattacca cctagtcgaa gccgaaacac aacaagacct
    15541 ccactatatc gaggaggcct tggagcagct cggttattta gtaaaggatt agaaaatttt
    15601 cttttcccaa tcgtcttctg tacgatgacg gtagaaaaac tctgatttgt ccggagcttc
    15661 catcatctcc atcagaggta gcatatcata ggccagatcc aagtttggaa tctggtcttt
    15721 ttgcacccaa gaaacttctc cctcttctga agagcgaagg gtaccagaga actcagtcgc
    15781 cttataacaa ataacaatgt agcgcccacc tgtatctagt ggccaatttt taatgccgac
    15841 aagttgagga ttttggatag tcaaccctgt ttcttcgtag atttcacgaa tgacagactc
    15901 cgcaaaagcc tcatcatttt ctacatgacc tccaggaaag gcataacctg accagcgatt
    15961 ggtgtcagga gagcgatact gcatgactac gcgctgagtt tctaagtctt caatcagaca
    16021 aatattggtt aaaatcgtta attgggaacg ggacataaat ttactcgctt tctaatttat
    16081 taaattttca gacttggtac cagtgccatt agcttctact ttttaaaatt attttagaat
    16141 agaataccat tcgtttttcc tttataggct ttccaagttc aagtatcttt tcttgtccat
    16201 caaaagtaaa acccattttt tgatagaaag caatggctcg cttgttatct ttcaataccc
    16261 ataagaaaat ttcagaaaaa tgattcagag cagtcaaagc tgctttcatt aacttttgtg
    16321 caatgccttt tccgtaatag tcttttaaaa catataaagc aataatttca ccagcttgca
    16381 tagtctcatc acgaaagtta ccataactga taaaaccaac taccttcaga tcacctatcg
    16441 caatcaatgt attttctgga tacttttgac taaagagtcg acatctttct aatgtcattg
    16501 tctcctgaaa ttctgcaggc aaaaggtcat cataagcctc tctccacatt tgccagtgaa
    16561 caagggattt accttctatc tctttaggag tttccattga tttgataata accgtcattt
    16621 attttctccc agtcttctct caaaatacca tatttaatac tatcaaaata tttaccttga
    16681 taataacgaa cttttggaat atgagcttct tttttcattc ttaatttttc agcaagtttc
    16741 atcataccaa gattccctga ccaagttgtc aaacccagat gctccaactc caagtaatcc
    16801 tgaaacgtcc tatctatcca ctgcaacata gcaactttcc caataccagt gttccagaat
    16861 tttttatcat aaataccaat tcccaattcc atccatcttg tttgtttaca tacccaataa
    16921 cgcgaaacag tcccaactag tttatcatca acaaaaatac caaggcgatt tgagttgctt
    16981 aaaatggatt ctgatttttg tagttcgaat tctttaaaat ttgaaaaata ctgataatca
    17041 tcataatagg gagcatcata ctgtttccaa actggattaa gttgtgaata agccatttcc
    17101 cataaagaca tcaaatcttc tctctttaac tttcttaaat aaatcatact attcccctaa
    17161 tccaaggcct tgacttccaa catcatatca cggatctgag ccgccagttc aaagtcaagc
    17221 acttcgacgg cttcttgcat ttgtttttct agtttcttga cgagttcttt gcgctcttgt
    17281 ttgttgaggc tattgatgtc gacttccttg tcctcttcct tagcaactgc cttggtcacg
    17341 gcaatcaggt cacggatttc tttcttgatt gtctgtggta cgataccatg ctcttcatta
    17401 taggccatct gaattttccg acggcgggca gtttcatcga tagcacgttg catagactgg
    17461 gtaaccgtgt ctgcatacat gatgacatgg ccttcactgt tacgagcagc acgtccaata
    17521 gtctggatga gtccacgttc gttgcgaagg aaaccttcct tgtcagcatc gagaatagct
    17581 acgaggctca cttcaggaac gtcaattcct tcacggagca ggttaattcc gaccaagaca
    17641 tcaaagacac ccaagcgcag gtcacggata atctccgtcc gttccaaggt cttgatatcc
    17701 gagtgcatgt acttgacctt gatacccatt tccttgaagt agtcggtcaa gtcctctgcc
    17761 attttcttgg tcaaagttgt gataaaggta cgctcatttt tttcaacgcg ggcattgatt
    17821 tcacccaaga ggtcatcaat ctgtcccata gtcggacgga cttccacctc tggatccaag
    17881 agtcccgttg gacgaatgat ttgctcaatc actgtctcgg tctgttcatt ttcatagtca
    17941 ccaggtgtcg ctgaaacgta aacaatctga tgaacgtgac tctcaaactc ctcccgacgg
    18001 agaggacgat tgtccaaagc agacggcaaa cggaaaccat aattaaccag catttcttta
    18061 cgcgaacggt ctccattgta catgcccttg atttgcccta tggtcatatg actctcgtca
    18121 atcataatca agaaatcatc tgggaagaag tcgagaagcg tataaggagg ctctccttcg
    18181 ctccgtccat ccatgtggcg agaataattt tcaaccccat tggtatagcc catctcacgc
    18241 aacatttcga tatcatactc tgtccgctgt ttcaaacgct gggcttcaag cagtttacct
    18301 tccttttcaa agacagctaa ctgctcctcc aactcggcct gaatctttgc aatggcaact
    18361 tccatgtggt cgtcattggt cacaaagtgt gtcgctggga aaatcgctaa atgatccact
    18421 tctcccaaca cctgacctgt cagagcctca acttcacgaa tacggtcaat ttcgtctcca
    18481 aaaaattcta ctcgaaaggc atgttcatct cgggaagctg ggaaaatctc taccacatcc
    18541 ccacgaacgc gaaatcttcc gcgttggaaa tcaatatcat tacgttcaaa ctgaatatcg
    18601 accaagtcat tcaagagttt atcacgagaa atctctagac caggacggag actaacgaca
    18661 ctatcagcgt attccttggg cgaacccaaa ccatagatac aagagactga ggccacgaca
    18721 ataacatcat tacgctccaa aagggctgag gtagctgagt ggcgaagctt gtcaatctcg
    18781 tcattgacag aactatcctt ctcaatatag gtatcgctag aagggacata ggcctctggc
    18841 tggtaataat catagtagga tacgaaatac tcaactgcat tttcagggaa aaattcctta
    18901 aactccccat agagctgacc agccagagtt ttattgtggg caataaccag agttggttta
    18961 ttgactttag aaatgacctg actcatagta taggtcttcc ctgttccagt cgcccccatc
    19021 agaatctgag ctttttctcc cccctcaatg ttatccacca actgctcgat agcttggggt
    19081 tgatctcctg atggttgata ttttgatact agtttaaatt gattatctgt aatgtgattg
    19141 atcataagaa tcctctctcc atgtgctatt cctattttag catacttata gtattttcac
    19201 gaagaaaagg acggaccgaa gtcacatcct ttcattttct ttctttaatt tattagcaag
    19261 atagacaatc agcagtagta ataccagact tgtcataatg gaaccttcta tgccaaaaga
    19321 accacctgat agccaatctt gattgccttg tggtaaaaag gtcatcagag acgttcctga
    19381 cggttgacca ctaactaaaa tcccaaagag attaccctga gcaaaattcc aagcaccatg
    19441 aatacctgca acaccccaaa ctgtatcaat tttgagaagg taaagagcca tggcaactcc
    19501 gaataaaaag agatttacta gagatagagg ggtgagacct gcattcccca catgaagcag
    19561 agtaaaaagt acgctagata taagaatagt aagttttaga ttggttcttg aggccaattg
    19621 aggaagtagc caagcacggg ccaccacttc ttctgctgtc ccctgtaaaa tccaaaatgg
    19681 gatagtaaag acgacaaagg caagagaata aggattcaag tgaatggatt ccaaacgata
    19741 ttgacctaag accactaaac ctaacaaggt cagaagaaaa agtgccaggc ctagactaaa
    19801 tcctttcaga agattgctga ggcaattctc tctataaaat cccaaggttc gaataggtct
    19861 tttctcaact tttcttgtcc atctgaacac agtgttaaga ataaaaccaa aggccagtaa
    19921 tcctaaaatt aaacgaaaat cacttgtttt atccgtcaag ctctgatgca aggtctgcaa
    19981 gtagcttgca agattttgac cggcctctcc aaaatttcta gcaagtccga tgagagctaa
    20041 caaactaata ccatataaag tataagccac aaactctcct atgaagacaa aaacaaaggc
    20101 taacaagggg cttgtgtaaa tgtttaaact catttttgaa gcttggaagt ctttaaatat
    20161 tcttttgttc ttcatatccc cgtcctcttt tc
//
LOCUS       DAVKDZ010000033        19576 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000033 DAVKDZ010000000
VERSION     DAVKDZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19576
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.034"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..1038
                     /locus_tag="VWZ30_001179"
     CDS             <1..1038
                     /locus_tag="VWZ30_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9334933.1"
                     /translation="LLRKRRFKCYHCSKMMVAETSIVKKNHQIPRIINQKIAQKLIEK
                     TSMTDIAHQLAISTSTVIRKLNDFHFECKVRNLPEIMSWDEYAFTKGKMSFIAQDFEK
                     LNIITVLEGRTQAVIRDHFLKYDRAVRCQVKIITMDMFSPYYALAKQLFPCAKIVLDR
                     FHIVQHLSRAMSRVRVQIMNQLDRKSHEYKAIKRYWKLIQQDSRKLSDKRFYRPTFRM
                     HLTNKEILDKLLSYSEDLKHHYQLYQLLLFHFQNKEPEKFFGLIEDNLKQVHPIFQTV
                     FKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIK
                     KERTKFVLSRY"
     gene            complement(1103..1175)
                     /locus_tag="VWZ30_001180"
     tRNA            complement(1103..1175)
                     /locus_tag="VWZ30_001180"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1140..1142),aa:Lys,seq:ctt)
     gene            complement(1440..2711)
                     /locus_tag="VWZ30_001181"
     CDS             complement(1440..2711)
                     /locus_tag="VWZ30_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU9334934.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            3009..3224
                     /locus_tag="VWZ30_001182"
     CDS             3009..3224
                     /locus_tag="VWZ30_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334935.1"
                     /translation="MMELPSQEILIFTKQIRHWILSDQVISGKRKLFFREDIPKEILD
                     LYENIKSKLDFAYQEVYSNNGLKKYEK"
     gene            3214..3462
                     /locus_tag="VWZ30_001183"
     CDS             3214..3462
                     /locus_tag="VWZ30_001183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334936.1"
                     /translation="MKNRIIDVFEVVNRILVITVENPDFEDLRVNQFVKIGDKKYRVR
                     SGPMIHSTPPQSVLDRDTFTIDYTDDELLDKEAVFTTH"
     gene            3599..3778
                     /locus_tag="VWZ30_001184"
     CDS             3599..3778
                     /locus_tag="VWZ30_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU9334937.1"
                     /translation="MPMTQKEMVKLLTAHGWIKIRGGKGSHIKMEKQGERPITIPHGE
                     LNKYTERGIRKQAGL"
     gene            3815..4267
                     /locus_tag="VWZ30_001185"
     CDS             3815..4267
                     /locus_tag="VWZ30_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU9334938.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQT"
     gene            4558..5028
                     /locus_tag="VWZ30_001186"
     CDS             4558..5028
                     /locus_tag="VWZ30_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEU9334939.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFIMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            5047..6114
                     /locus_tag="VWZ30_001187"
     CDS             5047..6114
                     /locus_tag="VWZ30_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU9334940.1"
                     /translation="MKKIGTYLVYVLAFVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVGITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENWLAELEKELAEGV"
     gene            6116..6274
                     /locus_tag="VWZ30_001188"
     CDS             6116..6274
                     /locus_tag="VWZ30_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-dihydro-8-oxoguanine-triphosphatase"
                     /protein_id="HEU9334941.1"
                     /translation="MEIEISDFTGCKIALFCGDKLLTILRDDKASIPWANMWELPGGD
                     CVIIMIGA"
     gene            6410..6583
                     /locus_tag="VWZ30_001189"
     CDS             6410..6583
                     /locus_tag="VWZ30_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334942.1"
                     /translation="MQKKEQSSRQVVVGYLMDVMSVDIEEANHLVSGLEHEGLVCFES
                     NGDVTVLVLEGQS"
     gene            6580..7407
                     /locus_tag="VWZ30_001190"
     CDS             6580..7407
                     /locus_tag="VWZ30_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEU9334943.1"
                     /translation="MKRITANHYQTSERYYKLPKLLFESERYKDMKLEVKVAYAVLKD
                     RLELSLSKGWIDEEGAIYLVYSNSKLMALLGCSKSKLLTIKKTLREYGLIDEVQQSSS
                     EKGRLANKIYLGELEDDTSPVLKSDGGGVKKTRGQSQKQTGQVLNSAPSETEISETKK
                     NETERNESVIEDEEEKKEVTFGKKQEESLTRKVDHVTKYDVGYIWNLVYERLRQEGLS
                     RVTSEYAMVHFEDRYRYALEHMRFVNTAEAIAEYVFNGVLSEWSKQIRLNEAKKGGW"
     gene            7567..8925
                     /gene="dcm"
                     /locus_tag="VWZ30_001191"
     CDS             7567..8925
                     /gene="dcm"
                     /locus_tag="VWZ30_001191"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEU9334944.1"
                     /translation="MKFLDLFAGIGGFRLGLTRQGHECIGFCEIDKFARKSYKVIYDT
                     EGEIEFHDIRQVTDQDFRQLRGQVDIICGGFPCQAFSLAGRRLGFEDTRGTLFFEIAR
                     AAKQIQPRFLFLENVKGLLSHDKGETFATILSTLDELGYDIEWQVLNSKDFQVPQNRE
                     RVFIIGHSRRYHPRFLFPLRGENSSAHLERLGNVNPSRKGMNGEVYLATGLSPTLTQN
                     KGEEAKIAIPVLTPDRLEKRQHGRRFKDNQDPMFTLTVQDRHGVVVAGTLPTSFVQTG
                     RVYDQSGLSPTLTTMQGGDKVPKIICREEAPHLKIKEATKLGYAKATVGDSVNLAYPE
                     STKRRGRVGKGISNTLTTSDNMGVVVAALEYRKDKWYEVTGIILDGKLYRLRIRRLTP
                     RECFRLQGFPDWAYERAESVSSKSQLYKQAGNSVTVTVIEAIAREFRKIEEEEGHEII
                     TH"
     gene            8906..9334
                     /locus_tag="VWZ30_001192"
     CDS             8906..9334
                     /locus_tag="VWZ30_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334945.1"
                     /translation="MKLLHTKSIRDCTELEEVIHQAEVERIAETIASLPNYDCEIEVT
                     FEDDYHQEMNVPLAYESNLHRLFEFMETQDLKNGVDSYLAEENHLVFRAFGQHYMANG
                     KDDLLTTRITVKSFGEGRTPIDMSKVFTPLTQKLPKDLSV"
     gene            9344..9727
                     /locus_tag="VWZ30_001193"
     CDS             9344..9727
                     /locus_tag="VWZ30_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334946.1"
                     /translation="MLEIYLSRNTSRNQNLLSFYDTHGISYTPKEVNDLSREDLLSLF
                     TKSSDCFALLSPSLQRFKGHREMKLSELVTLVLRKPDQNLRLPLVICEDKVYPDMSLE
                     EARTFLPRTHKVVSFREHLFKQLTE"
     gene            9737..9970
                     /locus_tag="VWZ30_001194"
     CDS             9737..9970
                     /locus_tag="VWZ30_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9334947.1"
                     /translation="MNDRFWENLESIVIERGMSWADLARKMFKGQYVYPSEFKRLYQT
                     FRHYKSHHLMPQVKWVEKVVSVLEIDYEDLFRR"
     gene            9973..10554
                     /locus_tag="VWZ30_001195"
     CDS             9973..10554
                     /locus_tag="VWZ30_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9334948.1"
                     /translation="MRRILLAYGAIHVNVLLVSFFLVGVLNEVVLIALQVSFFALVLW
                     VWKCFKIPKRNLSLKERGLWLLVSLGVMVSTALIMSAIFSGYAVNQETLVTVQDQAPM
                     LPFILFLLNASVVEEVFYREVLWGVLSQPVVQFLLTSFLFTLAHHPSSLITWGLYGSL
                     GLVLGLVRLKADCFTSTLVHLSWNGIVFFLSLL"
     gene            10630..11118
                     /locus_tag="VWZ30_001196"
     CDS             10630..11118
                     /locus_tag="VWZ30_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334949.1"
                     /translation="MSSEQQERQALQYAERSTMFTVRVLLKLLEWSARQALAQDSAYK
                     IGVQKLEELLQSPYAIEWLNISKDILDKPIDVEKFKELMESENLPIAISWQGDYLYFY
                     AKDKSLLDKHLDKLLQKLVSNPEKLQGLTLDKTLDEEIAKAKEQIVMSEPSAVKAKEV
                     IL"
     gene            11118..12938
                     /locus_tag="VWZ30_001197"
     CDS             11118..12938
                     /locus_tag="VWZ30_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003086094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEU9334950.1"
                     /translation="MASGKKAFIFGVLGLAFGYFCHRLVLLYDSLPNQPSIERFAYLL
                     GEGQNQVLNPLWKGNFTGRSVLGFCFGFVTMGLVYLYVSTGQRVYREGAEYGSARFGN
                     NRERKAFISKNPLNDTILSRNVRLTLLEKKAPQFDRNKNLVVIGGSGSGKTFRFVKPN
                     LIQLNCSNIVVDPKDHLAEKTGKLFLENGYQVKVLDLVNMTNSDGFNPFRYVETENDL
                     NRMLTVYFNNTKGNGSRSDPFWDEASMTLVRAISSYLVDFYNPPGSTKEEADSRRKRG
                     RYPAFSEIGKLIKLLSKGENQDKSVLEVMFESYAKTYGTENFTMRNWADFQNYKDKTL
                     DSVIAVTTAKFALFNIQSVIDLTKRDTLDLKTWGTQKTMVYLVIPDNDTTFRFLSALF
                     FSTVFSTLTRQADVDFRGQLPIHVRSYLDEFANCGEIPDFAEQTSTVRSRNMSLVPIL
                     QNIAQLQGLYKEKEAWKTILGNCDSLLYLGGNDEETFKFMSGLLGKQTIDVRSTSRSY
                     GQTGSGSTSHQKITRDLMTPDEVGNMKRDECLVRIAGVPVFKEKKYFPLKHKNWKYLA
                     DKETDERWWHYHIAPLKHEEESLDLSDHRVRDLSTETTLH"
     gene            12956..13198
                     /locus_tag="VWZ30_001198"
     CDS             12956..13198
                     /locus_tag="VWZ30_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9334951.1"
                     /translation="MNQKLTGFVYGVDASSMFAQAMALLQKGMIATGAFLVVMGIINL
                     STNIKDGGPGVRNAILEIVGGVMVGAAGAFITQITI"
     gene            13218..14069
                     /locus_tag="VWZ30_001199"
     CDS             13218..14069
                     /locus_tag="VWZ30_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HEU9334952.1"
                     /translation="MNTLPSAFVFLASEKISSDSLFEGFNVDLESTANLIKSLADYNP
                     TVWSYMSAITKGIMQPLGVAILAVVLVLEFSKMAKKIANSGGAMTFEAIAPMMVSYIM
                     VAVVITNTTVIVEAILAAASHIIEGVAGVVSHGGATYETISGLKGSGIIGKLIVGFFA
                     ILIWLVRLVSVMVVNLLITIRFIQLYLMIPFAPLTIPTFLSDDWRSVGIGYLKNIMVY
                     ALQGVLIFLIISLVPLFESAGKLAVSNGAGVMETLATTFGGLVQAILLIIALVGSQRT
                     ARSILGM"
     gene            14259..14612
                     /locus_tag="VWZ30_001200"
     CDS             14259..14612
                     /locus_tag="VWZ30_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEU9334953.1"
                     /translation="MNTRVFKDITKVQHRAWLGFTTRQVIFVLPAIALTILILGLNLF
                     YWQFGDWIVYGLIFTFTIPLMLFGVYRPNDLPFETYLNYRWHYEMTIPNRTLTGQKGI
                     RREKNKPLNETKDFF"
     gene            14590..16920
                     /locus_tag="VWZ30_001201"
     CDS             14590..16920
                     /locus_tag="VWZ30_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9334954.1"
                     /translation="MKPKTSSKHKKTGKPKEKVLPTTANTLRYQGLFPNGLMQVTSDY
                     FSQSYLLGDVNYQTVGLEDKGAIMETYSDLINSLDDKTNFQLTIFNQRVNLDKFRKSV
                     LYPMHEDGYDTYREELNRIMENNLEAGENNFSAIKLISFGKSDQNPKLAYRSLSQIGE
                     YFKSGFSEIDANFTLLNGESRVNHLADMLRGENHLPFTYQDLVRSGQTTKHFIAPTSL
                     SFKHKNYIEIDDRLLQIVYVRDYGMELGDKFLRELMQSDLEVVISLHAKGSAKSEAMT
                     KLRTKKTLMESQKIGEQQKMARSGVYLEKVSQVLESNIDEADELIKTMTQTGDKLFDT
                     LFLIGVFADNADRLKHSLDIIKQVSGSNDLIIDNLSYMQEAAFNSLLPFGKNYLEGVS
                     RSLLTSNIAVNSPWTSVDLQDKGGKFYGINQISSNIITIDRGKLNTPSGLILGTSGAG
                     KGMATKHEIISTKLKEADTDTEIIIVDPENEYSIIGQSFGGESIDIAPDSTTFLNVLD
                     LSDDNMDEDPIKVKSEFLLSWIGKLLDRKMDGREKSLIDRVTRLTYKHFETPSLVEWV
                     FVLSKQPEQEAKDLALDMELYVEGSLDIFSHHTNIKTDSHFLIYNVKKLGDELKQIAL
                     MVIFDQIWNRVVKNQKLGKKTWIYFDEMQLLLLDKYASDFFFKLWSRVRKYGAIPTGI
                     TQNVETLLLDANGRRIIANSEFMILLKQAKNDREELVHLLGLSKELEKYLINPEKGAG
                     LIKAGSTVVPFRNKIPHQTKLFDIMSTDPEKMRTEA"
     gene            16917..>19576
                     /locus_tag="VWZ30_001202"
     CDS             16917..>19576
                     /locus_tag="VWZ30_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU9334955.1"
                     /translation="MTSDKQNVKVARKTFQSSLKASRIHYRREKKGLKRSLPKRRFII
                     RRAEKAETREQRQTLKQTYQEETDLATDTFTKAISYVSPRWLKVKDIRKYRLPQARHR
                     LTVARKHLAENKMAEKEQAINPKFTYQKEPNQKTPSRFHFQKEKSLDRLQAEKKVNSA
                     KRDVKQLKRAHKAKKASTKFKRGLRYVASDSLDLVAQDDDLEGIRTVKDMSIKGRRYG
                     RFIYQSGKLLVTSGQTGMRFTKTKIAHGKERYQNFKKGKGFTRQKPLKHKRRYHTFLK
                     QARKHSVSGIKGVIQAIKGSLTFFSTIVLNPMTWVVSGLLFFLLLMMSFVVGISGTSL
                     IQQDEGELTKAYTHMTWEDAENTRTNDTGITYYTKIDDVMGFMNLKYQDYALEEVMEN
                     GDKTYQVYLSQLWHDLNGGDSLKAMLELTKEPAYKLSDDDREELKELSKEGTYLALQE
                     LDNPFQGQTEDDTLTMTVRYGYEMIEDKPTLHHHIILEAKENQVIVAPMDGKVSLDGE
                     NLIITSGKGLNKSQLTLFNVHTGRVTDGQKVQAGEMIGQTKDGSGLKVTYQKVDNDSE
                     KLVYVNPAFYFPKVIQLQTTILPTIGQFGGDEFARAKVIYEYLKSQGATNQAIAAILG
                     NWSVESSINPKRAEGDYLSPPVGATDSSWDDEGWLSLNGPAIYNGRYPNILRRGLGLG
                     QWTDTADGSRRHTLLLKYAKRQNQKWYDLDLQLDFMLHGDNPYYTNWLKDFFKNSGSP
                     ASLAQLFLIYWEGNSGDKLLERQTRAAEWYYQIEKGFSQPNGGTAQSDPKALEAVRED
                     LYDNSIPGGGDGMGYAYGQCTWGVAARINQLGLKLKGRTGEKISIINTMGNGQDWVGT
                     AARLGGETGKLPKAGAIISFAGGQYGHVGFV"
BASE COUNT         5876 a         3700 c         4470 g         5530 t
ORIGIN      
        1 ctacttagaa agcgtcgatt caagtgctat cattgctcga aaatgatggt cgctgagact
       61 tctatcgtca agaaaaatca ccaaattcct cgtattatca accaaaagat tgcgcaaaag
      121 ttgattgaaa aaacttctat gaccgatatt gcccatcaac tggccatttc aacttcaact
      181 gtcattcgca agctcaatga cttccacttt gagtgtaagg ttagaaatct gcctgagatt
      241 atgtcttggg acgagtatgc cttcactaag ggaaagatga gtttcattgc tcaggacttt
      301 gaaaagctca atatcatcac tgttcttgag gggagaacgc aagctgtcat tcgagatcac
      361 tttcttaaat atgatagagc cgtccgctgt caggtgaaaa ttattaccat ggatatgttt
      421 agcccttatt atgccttggc taaacagctt tttccatgtg ctaaaatcgt tctagatcgc
      481 tttcacattg tgcaacatct tagccgtgct atgagtcgtg tgcgtgtcca aatcatgaac
      541 caattggatc gaaaatctca tgaatacaag gctatcaagc gctactggaa actcatccag
      601 caagatagtc gtaaactgag tgataaacga ttttatcgcc ctacttttcg tatgcacttg
      661 accaataaag agattctaga caagcttttg agctattcag aagacttgaa acaccactat
      721 cagctctatc agctcttgct ttttcacttc cagaataagg aaccagagaa atttttcgga
      781 cttattgagg acaatcttaa gcaggttcat cctatttttc agactgtctt taaaaccttc
      841 ctcaaagata aagaaaagat tgtcaacgcc cttcaactac actattctaa cgccaaactg
      901 gaagcgacca ataatctcat caaacttatc aagcgcaatg cctttggttt tcggaacttt
      961 gaaaacttca aaaaacggat tttcatcgct ctgaacatca aaaaagaaag gacgaaattt
     1021 gtcctttcta gatattagct gacttcaacc cactacagtt gacaaagagc ctgaaaccaa
     1081 ggcttttctt gttgtatgta gatgccccct acagggatcg aacctgtgac ccacggatta
     1141 agagtccgct gctctgccag ctgagctaag gaggcaaaga aaaaagctgt attggtgccg
     1201 aaacttcacg gtttgtattg aacccgcgca attaagcagg tgggcaactc gctctaactg
     1261 aagctgtttc cgtgtgagac ggcctacatg ctgttagaag acttttgttt ccctaataat
     1321 acaaaaaata gtcggtcaac acttaagtgt gaagtcgtac accacagcgt ttctatattt
     1381 atatgatacc actttttcaa aaaaaatcaa gaggaaagtg caattttttg aaaaggattt
     1441 cagatttttc gcaaacggtc gatagcttcc tttctttgag ccaaagcccg ttcatattta
     1501 ccagtatctt ctgcttggaa atagtgatga ttacgaattt tttctggcag atagtcttgc
     1561 ttgacccaat ttccaggata gttgtgtgga tagagatagt cttgggcatt ccccagttcc
     1621 ttgcttccac tgtagtgccc atcacgcagg tgtcgcggaa taggcaagtg ccctgatgtt
     1681 ttgagatcag caagtgcctt atccatagct acataggctg agttggattt tggagaaagg
     1741 gccaaatcaa tcacgacatt ggcaatgaga atgcgggctt ctgggaaccc aatcttctgt
     1801 gcagcatcca gagcagtcac ggtatgaatc tgggcctcag gattggctaa gccgatatct
     1861 tcataggcga taacagtcaa gcgacgagcg agactaggca gatccccagc ctcaatcaag
     1921 cgggcagcat agtggagact ggcatccaca tctgagccac ggatagactt ttgcagggca
     1981 gatagaacat catagtgacc gtctccatcc ttgtccatag tgatgtagct tctctgaaga
     2041 ctattttcca tgatgtctaa ggtgatgtgg cgaatgccct cgtcattctc aggggtagag
     2101 agaacagcca agtccagtga gttaaaggca gagcgaaggt ctccgtttgt agaggttgcg
     2161 atgaaatcca gcgcatcctc atctagttct attggaaaat caaaaccacg ttcagggtta
     2221 cttagagcta tctgcagggc ctctttgacg tcttggttag acagaggttc caactcaaaa
     2281 atttgaacac ggctacgaat ggcaggagtg acggaaaaga aaggattttc agtcgttgct
     2341 ccaatcatga tgaccagtcc actttccaag agaggcaaga ggaagtcttg cttggtctta
     2401 tctagtcgat gaatttcgtc tagcaatagg acgagaccac cagaaaattt agcttcttcc
     2461 gagatttctt gcagtcgctt tttactatca actgtcgcat tgaaagttcg aaaggcatac
     2521 ttggtcgttc cagcgatggc agaggcaata ctggttttgc cgattcccgg agggccatag
     2581 agaatcatgg aggacaggcg gttggcttcc accatgcggc ggatgatttt tccaggtccg
     2641 accagatgct cctgaccgat gacctggtcg atggttttag ggcgcatacg aagcgcgaga
     2701 ttgtctggca tagcagtcct ttctaacgtg gatttgagta gaatgactca tctactatat
     2761 cttactatgt ccattctagc ctattagttg tgatatttca atggttttgt aataatcagg
     2821 gagtgagata gaatcttttt tactatttaa tttaataagg agaggtatca gcatgagaga
     2881 tttgattttc cttaaagctc aaaataggat agttgagcct aaagaagtgg aagtgctgac
     2941 ttgagaaata atattgctaa atgtcattgt atgaactata atgaaaagag aggtgataag
     3001 caatggaaat gatggaacta ccaagtcagg aaattttgat ttttacaaaa caaattcgtc
     3061 attggattct tagtgatcaa gttatttcag ggaaaagaaa acttttcttc cgggaagata
     3121 ttccaaaaga gattttagat ttgtacgaaa acattaaatc taaacttgat tttgcttacc
     3181 aagaagttta ttctaataat gggttgaaaa aatatgaaaa atagaataat tgatgttttt
     3241 gaagtagtaa atcgcatcct tgtaataact gttgaaaatc ctgatttcga agacttgaga
     3301 gttaatcagt ttgtaaaaat tggagataaa aagtatagag tacgcagtgg tcctatgatt
     3361 cattcaactc cgcctcaatc tgtcttagat cgagatactt ttacaattga ctatacagat
     3421 gatgaattgc tcgataaaga agcagttttt actactcatt agaaaaatag gtatgaataa
     3481 attggcttcc cttagaacca catctagggg cttttatgta taatagaata ataagtatca
     3541 ctcctttaca atacgtgtaa cacgtgttat aatggtgttg tcaggaggta aaagcgctat
     3601 gcctatgaca caaaaagaga tggttaaact ccttacggcc catggttgga taaagattag
     3661 aggcggtaaa ggttcccata ttaaaatgga aaagcaaggg gaaagaccta tcacaatccc
     3721 tcatggtgag ctgaataagt acactgaaag agggataaga aagcaagctg ggttgtaaaa
     3781 ttcagcccct gctttttgat atggaggtag tgaaatgtta gttacgtatc cagctttatt
     3841 ttattatgac gacacagacg gaacagaagc gacttatttt gtccatttcc cagattttga
     3901 atactcagct acacaaggag aggggatttc tgaggctttg gctatggggt cggagtggct
     3961 agggataact gttgcagatt tgatagagag tgatggagag ttacctcagc catcagatat
     4021 caatagcttg tctttaattg ataatgatcc ttttaaagat gatgaagatt tcgtgtcaac
     4081 ctatgacctt gataaatctt ttatttctat ggtatctgtt gatgtatcag aatacctagg
     4141 aagtcaggaa cccattaaaa agactttaac cataccaaaa tgggcagata agttgggacg
     4201 agaaatggga cttaactttt ctcagacttt gacagacgct attgcagata agaaagttca
     4261 aacctaagat taagcgtggt atcgtggtaa tagtcaataa aaaagcacgt ccacttgtgc
     4321 tagttactcg cctattgatt ttatagattt attagccctt tcgagggctt tttatattga
     4381 tttttatgag aaatgaagaa atcagacttt taagaaaatc actgatacca agggtttaaa
     4441 tgaggtaata tggtataatt aggacataaa ataattttgt ggtaagatgg tagtatctat
     4501 tctagcatat ttccgagcaa tcggggcgat taaagagtcg catagaaaga ggacaaaatg
     4561 gcaacatacg gatttttaga tgttttagag gaagagttgg acaagaactt tccctttgac
     4621 tttgagatta gctgggacaa acgcaaccat gcggtagaag tgagttttct attggaagcg
     4681 caaaatgctg caggtgtgga gatggtggat gaagacggag aggtttcatc agatgacatt
     4741 ctttttgagg aagcggtcct tttctacaat cctgccaagt caacagtcaa tgaggaagac
     4801 tatttgactg ttattcctta cctgcctaaa aaaggatttt cccgtgaatt tttagcttat
     4861 tttgcgcttt tccttaaaga tactgctgag gttgggctag atgtcctcat ggactttttg
     4921 gaagacccag aagcagagga atttatcatg gaatggaacc aagaagtctt tgaagaagga
     4981 aaagttggct tggaagaggg agaattttat ccttatccaa gatactagga gttggttgga
     5041 ggtgttatga agaaaattgg gacttatttg gtttatgtgc tagcttttgt ctttattatg
     5101 ctagcttttg cttgtggaac aatcgcattt gcagagttgg ggtattccgc agttttagcc
     5161 tttacttttg gttatgcctt cgctcttcta agcatgtatt taatctttat tcttcatgag
     5221 ctgggtcatg ctttttgtgg ctacttgaca ggctatcggc tggtggcttt tggattagga
     5281 cattttattt tgaccaaaaa gtcaggcagg tttcatctta gtagaacagc cattctgaaa
     5341 aatgttggtg ctcaatacat tggtttaaaa gaggatgaaa gcgatcaaag aatcatcctg
     5401 atgctttcag gaggcttgat ggttcatctc ggcttgatat tattggcgat agtgtttgga
     5461 tttttgacaa gaagctggta ttttgcaggg acttggattt ttcttaattt atctttcttc
     5521 ctaaataaca ttttaccagt cggcatcacc gatggagcaa aaatttggga attactacaa
     5581 caccctgaaa ataccaaata cgcctacctg gtattgagac attctgctca aactttgctg
     5641 gctcctcaag aatatgattt gaaagacttt gttcaagcag ttcctgagga tgcacgaggg
     5701 agttttgcgg atggtgtatt aggtatgcaa ggtgaagttt ccattttgga agggaaggaa
     5761 gaagtagcta aacagcagtt tcaagcatta cttgagagga caactactcc gatgatgcag
     5821 acagttgccc aactatccct gcttcatatc gctttgttgg aaggagattt tgagcaagcg
     5881 gagcaatatg ctagtattcg acaggtcaaa tcgtttctgt ctctcaaact gtccaatctt
     5941 caagtgatac aagcctggta tcaattaaag gtgaagaaag atgtagatcg aactcgtaag
     6001 gctctcaaga ttgctagaca gaaaatggat agtagtcgaa tgttgaggga tgagaaaaga
     6061 tattatgaaa actggttagc cgagctggaa aaggaattag ccgaaggagt ctaatatgga
     6121 aatagagatt tctgatttca caggttgcaa gattgctttg ttttgtgggg ataagctttt
     6181 gactatctta cgcgatgata aggcaagcat tccctgggcc aatatgtggg aactgccagg
     6241 tggggattgt gtcataataa tgataggtgc ttaaaacgct gataaatcaa ggatttgagc
     6301 gttctacaat ctgtcattaa ggaggaaatt tttgttttaa taagagaata tcgctatagc
     6361 gagaacattt taataggtgt tctcgctttt ttattttcag gaggaaaata tgcaaaagaa
     6421 agaacagtca tcacgtcaag ttgtggtggg ttatctcatg gatgtcatga gtgttgatat
     6481 tgaggaggcc aatcacttgg tctcaggctt ggaacatgaa ggcttggttt gctttgaatc
     6541 aaatggagat gtcacagtat tagtattgga gggacaatca tgaaacgcat taccgcaaat
     6601 cactatcaaa cgtcagagcg ttactataag ctcccaaaac tcttgtttga gagtgagcgt
     6661 tataaggata tgaaactaga agtcaaggta gcctatgctg ttttaaaaga tcgtttggaa
     6721 ttatctttga gtaaaggctg gattgatgaa gagggggcta tttatttggt ttattccaac
     6781 tcaaaactga tggctctcct tgggtgttcc aagtcaaagt tgctgacgat taagaaaacg
     6841 ttaagagagt atggcttgat tgatgaagtc caacagtctt ctagtgaaaa agggcgcttg
     6901 gccaataaga tttatttagg ggagttggaa gatgacacta gccccgtctt aaaatcagac
     6961 gggggtggtg ttaaaaaaac acgggggcag tctcaaaaac aaacggggca ggtcttaaat
     7021 tcagccccta gtgagactga aataagtgag actaagaaaa atgagactga aagaaatgag
     7081 tcagtcattg aggacgagga ggagaaaaaa gaagtcactt ttggaaaaaa acaagaagaa
     7141 tctctcactc gtaaagttga tcatgtgaca aaatacgacg taggctatat ttggaatttg
     7201 gtctatgaac gcttgcgaca agaaggcttg tctcgagtta cgagtgaata tgcgatggtt
     7261 cattttgagg atcgttatcg gtatgcttta gagcacatgc gttttgtaaa taccgctgaa
     7321 gcgattgctg aatatgtctt taatggggtt ctatccgaat ggagtaagca gatacgacta
     7381 aatgaagcga agaaaggtgg ttggtgaaca gatgttttgg tggatcgtat taggaatctg
     7441 gagactaggg ttgatttggc taattgttgc aatcatcacg gcaccagaca tggacgatta
     7501 taattgaggt aggacgttac gtaaaatgta aggtcctttt agttttgtca gaaaggaagg
     7561 agagacatga agtttttaga tttatttgct ggaattggag gctttcgact ggggttaact
     7621 cgtcaaggcc atgaatgtat tggtttttgt gagattgaca agtttgcgag gaaatcttac
     7681 aaagtcatct atgatacgga aggagaaata gaatttcatg acattagaca agtcacagac
     7741 caagacttta gacaactcag agggcaggtg gacatcatct gtgggggatt cccttgtcaa
     7801 gcattttcac tcgcaggcag acgattggga tttgaggaca ctaggggtac tctgttcttt
     7861 gagattgctc gagcggccaa acagatccaa ccacgttttt tattcctcga aaatgtcaag
     7921 ggtttactca gtcacgacaa gggagagacg tttgccacaa tcctctctac attggatgag
     7981 ctggggtatg atatcgaatg gcaggtgctt aacagtaaag atttccaagt cccacaaaac
     8041 cgagaaaggg tctttattat cggacattct agaagatacc atcccagatt cttatttcct
     8101 ctcagaggag aaaatagctc agctcatctt gaaagattag gaaatgtcaa tccgtccaga
     8161 aaaggcatga acggtgaggt ctacctagca acaggtctct cgcctacttt gacgcaaaat
     8221 aaaggagaag aagcaaagat tgccattcca gtcttaaccc ctgatagact ggagaaacgc
     8281 caacatggca gacgttttaa ggacaatcaa gaccccatgt ttaccctaac ggttcaagat
     8341 agacacggtg ttgttgtcgc aggaaccctt ccgacctctt ttgtgcaaac tgggcgagtc
     8401 tatgaccagt ctggcctatc ccctaccctt accaccatgc aaggagggga taaagtgcct
     8461 aagattatct gccgtgaaga agctccgcat ttgaaaatca aggaagcgac caaattaggc
     8521 tatgctaagg caacagtggg agattctgtc aaccttgctt atccagagtc aaccaaacgc
     8581 agaggacgag taggaaaggg aatctcaaat accttaacca cttctgacaa tatgggagtg
     8641 gtggtagctg ctcttgaata ccgtaaggac aagtggtatg aagtgacagg aataatccta
     8701 gatggaaaac tttatcgttt gagaatcaga cgattgaccc ctagagagtg ttttagactt
     8761 caggggtttc ctgactgggc ttatgaaaga gcagaaagcg tatcaagtaa aagtcagttg
     8821 tacaaacaag caggcaatag tgtgactgtc accgtcattg aagctattgc tagagaattt
     8881 agaaagattg aagaggaaga aggacatgaa attattacac actaaaagta ttagggactg
     8941 tacagagctg gaagaagtta ttcatcaagc agaggtagaa aggattgctg agacgattgc
     9001 ttctttaccc aactatgatt gtgagattga ggtcactttt gaagatgatt accatcagga
     9061 gatgaatgtt ccattggctt acgagtccaa cttacatcga ctctttgagt ttatggaaac
     9121 gcaagacctt aaaaatggcg ttgacagtta tctagcagaa gagaaccatc tcgtttttag
     9181 agcttttggt caacactata tggcaaatgg aaaagacgat ttattgacca cacggattac
     9241 agtaaaaagt ttcggtgaag gcaggacacc cattgatatg agtaaggtgt ttactcctct
     9301 tactcaaaaa ttaccaaagg atttatcggt gtaggaggaa gaaatgctag aaatctattt
     9361 aagtcgaaat accagtcgaa atcaaaatct cctttctttt tatgacacgc atggtatttc
     9421 ttacactcct aaagaagtga atgacttgtc acgtgaggac ttactgagct tgtttaccaa
     9481 aagcagtgat tgttttgccc tcttatcccc ttctctacaa cggtttaaag gtcatagaga
     9541 gatgaaatta agtgaattag tcacgttggt tctcaggaaa cctgatcaga atttgcgatt
     9601 acctctggtg atttgtgagg ataaagtgta tcccgatatg agtttagaag aagcgagaac
     9661 ctttctacca agaactcata aggtggtgtc ttttcgtgag cacctcttta agcaactaac
     9721 agaataagga ggagctatga acgaccgctt ttgggaaaat ttggaaagta tcgttattga
     9781 aagaggcatg tcatgggctg atctcgctcg aaagatgttt aagggtcaat acgtttatcc
     9841 tagtgagttt aaacgtctct accaaacctt tcgtcactac aaatctcatc acctcatgcc
     9901 acaagtcaaa tgggtggaga aagtggtgag cgtcttagag attgactatg aagacttgtt
     9961 taggaggtag acatgagaag gatattgctg gcgtacggag ccattcatgt taatgttctt
    10021 ttggttagtt tctttttggt aggagtgcta aatgaggtag tgctaatagc cttacaagtt
    10081 tctttttttg ctcttgtact gtgggtctgg aaatgtttta agatacctaa aagaaattta
    10141 tccttaaaag agagagggct ttggttgctt gtcagtcttg gtgtcatggt gagtacggct
    10201 cttatcatga gtgccatctt ttcaggatat gcggtgaacc aagaaacgtt agtgacggtt
    10261 caagatcaag caccaatgtt gccttttatc ctctttctcc tcaatgccag tgtggtggaa
    10321 gaagtatttt acagagaagt gctgtgggga gtcttgtctc aacctgtggt tcagtttctg
    10381 ttaacgagtt tcctctttac tttagcccat cacccatcta gtctcatcac ttgggggctt
    10441 tatggtagtt taggactggt gctaggacta gttcgcttaa aggctgattg tttcacctca
    10501 accctcgttc atttgtcttg gaatggaatt gtctttttcc tgtctttgtt gtgataccaa
    10561 aatcaagggt actttcgtat catgaaagca ccctagtgtt tataagggaa tcatgccaag
    10621 gagggaatga tgtcatccga acaacaggaa cgccaagcgt tacagtacgc agagagaagc
    10681 actatgttta ccgttagggt tctcctcaag ctcttagagt ggtccgctag acaagctttg
    10741 gcacaagact cggcctataa gattggggtt caaaagttag aagaactcct tcaaagccct
    10801 tatgccattg agtggcttaa tatctcaaaa gacatcttgg ataaacccat tgatgtcgag
    10861 aaattcaagg aactgatgga atcggagaat ctgcctattg ccattagttg gcagggagac
    10921 tacctttatt tctatgctaa ggataaatct ttgttagata agcacttgga taagttgctt
    10981 cagaagttgg tttccaatcc cgaaaaactg caaggcttaa ctctggataa aaccttagat
    11041 gaggaaatcg ctaaagccaa ggaacagatt gtgatgtcag aaccttctgc tgtgaaggcc
    11101 aaggaggtaa ttctctaatg gcctcaggga aaaaagcttt tatctttggt gttttaggac
    11161 ttgcctttgg ctatttttgc catcgcctag tcttgcttta cgatagtctc ccaaatcaac
    11221 catcgattga aaggtttgcc tatctcttag gagaaggtca gaatcaggtt ttaaatccct
    11281 tgtggaaggg caactttact ggtagatcgg ttttagggtt ttgttttgga tttgtgacga
    11341 tgggactagt ctatctttac gtctcaactg gtcagagagt ctaccgagaa ggggctgagt
    11401 atggctctgc tcgttttgga aacaatcggg agcgtaaagc ctttatcagt aagaacccct
    11461 tgaacgatac tatcttatct cgaaacgtta ggctaactct cttagaaaag aaagccccac
    11521 aatttgaccg aaacaagaac ttagtggtca ttggtggatc aggatcaggg aaaacctttc
    11581 gctttgtcaa acccaatctg attcagctta actgttccaa tattgtcgtt gatcctaagg
    11641 accacttagc tgaaaagaca gggaagctct ttttagaaaa cggctatcag gtcaaggtct
    11701 tagacctcgt aaacatgacc aactcagatg gctttaatcc ctttcgttat gtggaaacgg
    11761 agaatgattt gaaccgtatg cttacggtct atttcaataa taccaaaggc aatggcagtc
    11821 gcagtgatcc cttttgggat gaagcttcca tgaccttagt gagagccatt tcctcttact
    11881 tagtggattt ttacaatcct cctggtagta ccaaggaaga agcagacagt cgccgtaagc
    11941 gtgggcgtta cccagccttt tcagaaattg gaaaactcat caagctctta tccaaagggg
    12001 aaaatcaaga taagagtgtc ttagaagtca tgtttgagag ctatgccaag acctatggaa
    12061 cggagaactt taccatgcgt aactgggcag acttccaaaa ctacaaggat aagaccctgg
    12121 attcggtgat tgctgtaaca actgctaagt ttgccctctt taatatccaa tccgtcattg
    12181 acttaaccaa gcgtgatacc ttggacttaa agacatgggg gacgcagaaa actatggtct
    12241 atctcgttat tccagataat gacaccacct ttcggtttct ctcagcactt tttttctcca
    12301 ccgtcttttc aaccttaacc agacaagctg acgtggactt taggggacaa ctgcctatcc
    12361 acgttagaag ttacttggat gagtttgcga attgtggtga aatcccagac tttgcggaac
    12421 aaacctcaac ggttcgctcc agaaacatga gtctcgttcc tatccttcaa aacattgccc
    12481 aacttcaagg actttataag gagaaggagg cttggaaaac gattctaggg aactgtgata
    12541 gtttgcttta tcttggtggc aatgatgaag agaccttcaa gttcatgagt ggtctcttag
    12601 gcaaacaaac cattgatgta agaagtacca gtcggtcgta tgggcaaaca gggtctggtt
    12661 ctacctccca tcaaaaaatt acgagggatc tcatgacccc tgatgaagtc ggaaacatga
    12721 aacgagatga gtgcttggtg agaattgcag gggttcctgt ttttaaggag aagaaatatt
    12781 tccctttgaa acacaagaac tggaaatatc tcgcagataa ggagacagat gagcgttggt
    12841 ggcattacca cattgccccc ttgaaacatg aggaggaaag tttggactta tcagaccaca
    12901 gggttaggga tttaagcaca gaaaccacac tacactaata gaaatgagga atcgtatgaa
    12961 ccaaaaatta acaggctttg tttatggcgt ggatgcaagt tccatgtttg cccaagccat
    13021 ggcactttta caaaagggaa tgattgcgac aggagccttt cttgtcgtga tgggcattat
    13081 taacctttca accaatatca aagatggtgg acctggtgtt cgcaatgcca tcttagaaat
    13141 tgtgggtggt gtcatggtgg gtgccgctgg tgcctttatc actcaaatta ccatttagga
    13201 gggctaggta tgacaagatg aatacattac cttcagcttt tgtctttcta gcctcagaga
    13261 aaatttcaag tgatagcctc tttgaaggct ttaacgtaga cttggaatcg accgctaact
    13321 tgatcaaatc ccttgcggat tataacccaa ccgtatggtc ttatatgtcg gccatcacaa
    13381 agggaatcat gcagcccttg ggagtggcta ttttagctgt tgttctagtc cttgagtttt
    13441 ctaaaatggc taagaaaatc gccaactcag gtggagccat gaccttcgaa gccattgccc
    13501 caatgatggt ctcttatatc atggtggctg tggtcattac aaatactact gtgattgttg
    13561 aagccatctt agccgctgct tctcacatca ttgaaggagt ggctggcgtc gtttctcatg
    13621 ggggagcgac ttatgagacc atttcagggt taaaagggtc aggaattatt ggaaaattga
    13681 ttgtcggctt ctttgccatt ttgatatggc tggttcggtt ggttagtgtc atggtagtga
    13741 atcttctcat taccattcgc tttatccaac tttatctcat gattcctttt gcgcctctaa
    13801 ccattccaac cttccttagt gatgactggc gaagtgttgg gattggctac ctgaaaaaca
    13861 tcatggtcta tgccctccaa ggagtcttaa ttttcttaat catctccctt gtgcccctct
    13921 ttgaatctgc tgggaaatta gctgtttcaa atggagcagg agtaatggag acgttagcga
    13981 ctacctttgg tggcttagtc caggctattt tattaatcat tgccttggtg ggcagtcaac
    14041 ggactgcaag aagcatccta ggcatgtgag agtaggacag gaatcggtac tgctttgcag
    14101 tcgaaggcgt cgtcttacct ccgcaaggac gactagaact gtcatagcta ggaactagcg
    14161 tagagagttc agacgtctac tgcgtcaaag acatgaaacc gttcaaactg gactgagatt
    14221 tccatcttag tcctttttca aagaaggagc tactatttat gaatacacgt gtttttaagg
    14281 acatcacgaa agtccaacat agggcttggt tgggctttac cacacgacaa gtcatctttg
    14341 tcttgccagc tattgccctc acaatcctta tcttaggact gaaccttttt tattggcaat
    14401 ttggggattg gattgtctat ggacttattt ttacttttac cattcctctc atgttatttg
    14461 gggtctatcg tcctaatgac ttgccctttg aaacttatct taattatcga tggcattacg
    14521 aaatgactat accaaaccgc acactaactg gacagaaagg aatacgccgt gagaaaaaca
    14581 aaccactcaa tgaaaccaaa gacttcttct aagcataaga agacaggtaa accaaaagaa
    14641 aaggtcttgc caacaactgc caataccctt cgttaccaag gactttttcc taatggtctc
    14701 atgcaagtca catctgatta tttttcccag tcctatcttt taggagatgt taattaccag
    14761 acggtaggac ttgaagataa gggagcgatt atggaaactt attcggactt gattaacagt
    14821 ttggatgaca agaccaattt ccaattaacc atttttaacc aaagagttaa cttggacaag
    14881 tttagaaaga gtgtcttgta tcccatgcat gaggatggct acgatactta ccgtgaagaa
    14941 ctcaatcgta tcatggagaa taacctagaa gctggtgaaa ataacttttc agctattaag
    15001 cttatttcgt ttgggaagtc agaccaaaat ccgaaactgg cttatcgctc tctttctcaa
    15061 attggggaat acttcaagag tggtttttca gaaatagatg cgaactttac tctcctcaat
    15121 ggagaaagcc gtgtgaatca cttggctgat atgttgcgag gggaaaatca tctgcccttt
    15181 acctatcaag acctggtgcg ttcgggacaa acgaccaagc attttattgc cccaaccagt
    15241 ctatccttta aacataagaa ttatatagag attgacgata gactccttca gattgtttac
    15301 gttcgtgatt atgggatgga attgggggac aagttcctac gtgaactcat gcagtctgat
    15361 ttggaagtgg tcattagttt acatgccaaa ggctcagcca agtcagaagc tatgaccaag
    15421 ctccgcacca agaagacctt gatggaatca caaaaaattg gggaacaaca aaagatggca
    15481 cgatcaggtg tctatcttga aaaggttagt caggtcctag aaagcaatat cgatgaagct
    15541 gatgagctga ttaaaaccat gacgcaaaca ggagacaagc tctttgacac cctctttttg
    15601 attggggtct ttgcggataa cgcagatcgg ttgaagcact cacttgacat tatcaagcaa
    15661 gtgtcaggtt ctaatgatct cattattgat aatctttcct acatgcaaga agctgccttt
    15721 aatagcttgt taccttttgg aaagaactat cttgaaggtg tgtctcggtc actcttgact
    15781 tcaaacattg ccgtcaattc cccatggact tccgttgatt tacaagataa gggtgggaaa
    15841 ttctatggga taaatcagat ttcaagtaat attatcacca ttgaccgtgg gaaactcaat
    15901 accccatcag gtcttatttt aggaacatca ggtgctggta agggaatggc aaccaagcat
    15961 gaaatcatct caaccaagtt aaaagaagca gatactgata ccgaaatcat cattgtcgac
    16021 cctgaaaatg agtatagtat tatcggccaa tcctttggtg gggaaagcat tgacatcgcg
    16081 cctgactcta ctaccttttt aaacgtctta gacctatctg atgataacat ggatgaagac
    16141 cctatcaagg tcaagtcgga atttcttctc tcttggattg gaaaactctt agaccggaaa
    16201 atggatggtc gcgagaagtc actgattgat cgtgtgacac ggctcaccta taagcatttt
    16261 gagacaccgt ccttagtaga atgggtcttt gtcctctcca aacaacctga acaagaagcc
    16321 aaggatttgg ctcttgatat ggaactctat gtggaaggct cacttgacat tttctcacac
    16381 cacaccaaca tcaaaaccga tagtcatttt ctcatttaca atgttaaaaa gttaggggat
    16441 gaattgaagc agattgccct catggtcatc tttgaccaga tttggaatcg tgtggttaaa
    16501 aatcaaaaac tagggaagaa gacttggatt tactttgatg aaatgcagct tctgttgctg
    16561 gataagtacg cttctgattt cttcttcaag ctctggagcc gtgtccgtaa gtatggggca
    16621 attccaacag ggattacgca gaacgtggaa actcttttgt tagatgccaa tggcagacgc
    16681 attatcgcta atagcgaatt catgattctc ttaaaacaag ctaagaatga ccgagaagag
    16741 ttggttcatc ttttgggctt atccaaagag ttggagaaat atttgatcaa tcctgaaaag
    16801 ggagctggat tgattaaggc agggtcaacg gttgttcctt ttagaaataa aatcccacat
    16861 caaaccaaac tctttgacat catgagcacc gatccagaaa agatgaggac tgaagcatga
    16921 catcggacaa acagaacgtt aaggtagccc gtaaaacctt tcagagtagt ttaaaggcta
    16981 gccgtattca ttaccgtagg gagaagaagg ggctgaaacg ctcacttcct aaacgacggt
    17041 ttatcatcag acgggcagaa aaagctgaaa cgagagaaca aaggcaaacc ttgaaacaga
    17101 cctaccaaga ggagacagac ctagcgacag atacctttac aaaagctatc tcctatgtgt
    17161 cacccagatg gctaaaggtc aaggacatca gaaagtatcg ccttcctcaa gccagacatc
    17221 gcttaacagt agcgagaaaa cacttggcag agaacaagat ggcagaaaaa gagcaggcta
    17281 tcaatcctaa gttcacttat cagaaagagc caaaccaaaa gactccttca cgatttcact
    17341 tccagaaaga gaaaagtctt gaccgccttc aggcggaaaa aaaggtaaac tcagcgaagc
    17401 gtgacgtgaa gcaactgaaa agagcccata aggctaagaa agcctctacg aaattcaaac
    17461 gagggttacg ctatgtcgct tcagactcac ttgatttagt tgctcaagat gatgacctag
    17521 aaggaattcg aaccgtaaag gatatgagta tcaaaggcag acgctatggg aggttcatct
    17581 atcagtcagg aaaattgctt gtgacaagtg gacagactgg tatgcgtttt accaagacta
    17641 aaatcgccca tggcaaggaa cggtatcaga atttcaaaaa ggggaaaggc ttcacccgtc
    17701 agaaaccctt aaaacacaaa agacgatacc acacttttct caaacaagct aggaaacaca
    17761 gtgtgtcagg aataaaaggg gttattcaag ccatcaaggg aagtctaacc ttcttttcaa
    17821 caattgtcct caatcccatg acttgggtcg tgtcaggtct cttatttttc ttgcttttga
    17881 tgatgagttt tgtcgttgga atttcaggca ccagccttat ccaacaagat gagggtgagc
    17941 tgacaaaagc ctatacgcac atgacgtggg aagatgcgga aaacaccaga accaacgata
    18001 ctggcattac ctactacacg aaaattgatg acgtcatggg ctttatgaac ctcaagtatc
    18061 aagattatgc tttagaagaa gttatggaaa atggggataa gacctatcaa gtttatttga
    18121 gtcagctttg gcatgactta aacggtgggg attctttaaa agctatgctt gagctaacca
    18181 aggagcctgc ctacaaactc tcggatgacg atagggaaga attgaaggaa ctaagtaagg
    18241 aaggtaccta ccttgcccta caagaattgg acaatccttt tcaaggacaa actgaagatg
    18301 atacgctaac tatgacggtt cgctatggct atgagatgat tgaggacaag ccaacgcttc
    18361 atcaccatat cattttagaa gccaaagaga accaagtgat tgtggcaccc atggatggca
    18421 aggtatcgct tgatggagag aaccttatta tcacgtcagg taagggtctc aataagtctc
    18481 aactgacctt gtttaatgtc catacaggtc gagtgaccga tggccaaaag gttcaagcag
    18541 gagagatgat tggtcaaacc aaagatggtt ctggtctcaa agtaacctat caaaaggttg
    18601 acaatgattc agagaaactg gtttatgtca atccagcctt ttattttcca aaggtgattc
    18661 aactccaaac gaccattcta ccgactattg gtcaatttgg gggcgatgag tttgcgagag
    18721 ctaaggtcat ctatgagtat ttgaaaagcc aaggtgcgac caatcaagcg attgcggcta
    18781 ttttagggaa ctggtcagta gaatcctcta tcaatcctaa acgagcagaa ggtgactact
    18841 tatcgcctcc tgtgggggca acggatagct cttgggacga tgagggatgg ttgtccctta
    18901 atggtccagc tatctataat ggcaggtacc caaatatcct aagacgtggc ttaggattag
    18961 gtcagtggac agatacggca gatgggtcac gcagacatac gctcttatta aaatatgcca
    19021 aacgacaaaa tcagaaatgg tatgacttag acttacaact ggatttcatg ttgcacggag
    19081 ataatcctta ttacaccaac tggttaaaag acttcttcaa aaattcagga agtccagcta
    19141 gtcttgccca actctttctc atttattggg agggaaatag tggcgataag ctcctagaac
    19201 gtcaaacaag agccgccgag tggtattacc aaattgagaa aggctttagc caacctaatg
    19261 gtggaaccgc ccaaagtgat ccaaaagcac ttgaagctgt tcgagaagac ctttatgata
    19321 attctatccc aggtggtggt gatggcatgg gctatgccta tggtcaatgt acttggggag
    19381 tggcagctcg gatcaaccaa ctaggcttga aactcaaagg cagaactggt gagaaaatct
    19441 caatcatcaa taccatggga aatggtcaag attgggtagg cactgcggca cgtcttggtg
    19501 gagagacagg aaagttacct aaagcaggag cgattatctc ttttgcgggt ggtcagtacg
    19561 ggcatgttgg gtttgt
//
LOCUS       DAVKDZ010000034        19438 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000034 DAVKDZ010000000
VERSION     DAVKDZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.035"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            103..2160
                     /gene="pbp2b"
                     /locus_tag="VWZ30_001203"
     CDS             103..2160
                     /gene="pbp2b"
                     /locus_tag="VWZ30_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEU9334956.1"
                     /translation="MRQICMRKFNSHSIPIRLNLLFSIVILLFLTIIGRLLYMQVLNK
                     DFYEKKLASASQTKVTTSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTAKDLKEIA
                     SQLLGYVSITSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEVYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKILETSLSSIVGSVSSEKAGLPAEEAESYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELGNGGAKYSEGVYAVALNPKTGAVLSMSGLKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQPIVFQGSAPIYSWYKLAYGSFPITAVEALEYSSN
                     AYMVQTALGIMGQTYQPNMFVGTSNLETAMGKLRATFGEYGLGAATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANDGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVAGGQEANNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            2171..2767
                     /gene="recR"
                     /locus_tag="VWZ30_001204"
     CDS             2171..2767
                     /gene="recR"
                     /locus_tag="VWZ30_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU9334957.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSDDDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            2943..3986
                     /locus_tag="VWZ30_001205"
     CDS             2943..3986
                     /locus_tag="VWZ30_001205"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU9334958.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSQTPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLTYPVFTKPSNMGSSVGISKSENQEELRQALKLAFQYDSRVLVEQGVIAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPDLIERLVDL
                     AKESFDKREAHLL"
     gene            4071..5441
                     /locus_tag="VWZ30_001206"
     CDS             4071..5441
                     /locus_tag="VWZ30_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU9334959.1"
                     /translation="MKLTIHEVAQAVGAKNDVSIFEDIQLEKAEFDSRLIEEGDLFVP
                     LKGARDGHDFIEIAFENGASVTLSEKEIANHPYILVDDVLTAFQTLAAYYLEKTAVDV
                     FAVTGSNGKTTTKDMLAHLLSTIYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELAHPKTAIVTLVGEAHLAFFKDRSEIAQGKMQIADGMASNSLLLTP
                     ADPIVEDYLPTDKKVVRFGQGAELEVTDLIERKDSLTFKANFLGQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIHQAFQNLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEYQDQFEDLVKQVKESLGTNDQILLKGSNSMNLAKLVE
                     SLENEC"
     gene            5431..6042
                     /locus_tag="VWZ30_001207"
     CDS             5431..6042
                     /locus_tag="VWZ30_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9334960.1"
                     /translation="MNASDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQGSDLDVEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            6133..6837
                     /locus_tag="VWZ30_001208"
     CDS             6133..6837
                     /locus_tag="VWZ30_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU9334961.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            7056..8429
                     /gene="ftsA"
                     /locus_tag="VWZ30_001209"
     CDS             7056..8429
                     /gene="ftsA"
                     /locus_tag="VWZ30_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU9334962.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            8446..9705
                     /gene="ftsZ"
                     /locus_tag="VWZ30_001210"
     CDS             8446..9705
                     /gene="ftsZ"
                     /locus_tag="VWZ30_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU9334963.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQVSAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            9710..10381
                     /locus_tag="VWZ30_001211"
     CDS             9710..10381
                     /locus_tag="VWZ30_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU9334964.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDIIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            10391..10930
                     /locus_tag="VWZ30_001212"
     CDS             10391..10930
                     /locus_tag="VWZ30_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU9334965.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            10930..11193
                     /locus_tag="VWZ30_001213"
     CDS             10930..11193
                     /locus_tag="VWZ30_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU9334966.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            11190..11975
                     /locus_tag="VWZ30_001214"
     CDS             11190..11975
                     /locus_tag="VWZ30_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU9334967.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPLAEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLKLLQDKGQTKKEKKKITVQLLLSK"
     gene            11984..12773
                     /locus_tag="VWZ30_001215"
                     /pseudo
     CDS             11984..12773
                     /locus_tag="VWZ30_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
     gene            13121..15913
                     /locus_tag="VWZ30_001216"
     CDS             13121..15913
                     /locus_tag="VWZ30_001216"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU9334968.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNSPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGETRKFVVAAELLISLSEK
                     FGWADVQVLATYRGQELNHIVTEHPWDTVVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWERDTKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDAVAYEELRSVDKYMT
                     IRFNQLVKTIRDAYANFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEAETFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTMAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAGGFEEK"
     gene            16096..>19438
                     /locus_tag="VWZ30_001217"
     CDS             16096..>19438
                     /locus_tag="VWZ30_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEU9334969.1"
                     /translation="MRQTNVRGSLSLRGFRKLRTGAIVATGVILLGLGFSAPTASVSE
                     QDGVWVARTVAEVKADIQKEGDLFKYVIKWGDTLSVISEASNIPVDVLQEMNQASGKN
                     LFLPYNELYFTEAETVSDTVTKHKIVIKESGASGEVRTYEVLLKKDQETKAVTFELTD
                     VTEKVEKMTVNYSPVYQPVSVPVVERAEESVEEPALVAPAVTSPEVKEETEDPVVLKA
                     EEAATTPATKETAPEVVTPAEPNAETPAPKVEAPTESSEPKGTEPEVAKPAGDSVSST
                     SESNEDGNSGDSTHSTLTSDETKPEEVESTSVTDNVPSNAGTTNQPLTPTSNSDDSAP
                     KEVANGTEGKVEAPKVEEAVSEGEEGNAEITNNPSTGATPSSDETTEQSGTPAPKPEE
                     EAPVATETSAASAESTESAPVVAKPAEPKAETPEATITGDEGKESEESGTSATVESTP
                     AGEEGKAEDSVSSTESSSTASEGESTATQPAVEAGTDSTSEAEELATTETATTPKTEE
                     PEAVAPAPEAPSESDSKEEDSAAGDTTVTEEQPAAETVSEGGSPATQPAGEEGKSEKE
                     PKVEGQPSGTESATPEQHTNIEETTEKQSGATSENSGDSLVSDEPAASSGISTSSGTE
                     GAETIDTETIAEKSEIPSPAEEGSAKEEKEVIKKLELRNISSVELYSKEGDKYRHVTS
                     LATLPSNPETYFMKVKSENFKDVMLPVTSITNDNKDNRDVYKIVASVKNLIQHENNKV
                     LENYTYYLSKTQQGETGVYTSFKNLVDAMNRDSYGEFRLGATMDAREVELPDGQESYV
                     KNVFHGRLIGQNSNKYYAIYNLKKPLFNALSNARVQNLSLKDVNISAKDDTATLAKEA
                     NNNTHIDNVHSDGAIAGERSIGGLVSQVNNSTISNSSYTGRITNTYKTVASYQIGGLV
                     GKLSGPNGLIDKSIASIDLASNATRGDQSIGGIAGSVIDNAVISSSYAEGKLNNVQPF
                     ANVGGVVGDLWDPVGGLEKSGQLSNVLSDVNVTNGNAIAGKHFDHMKATNVYSNKNNK
                     VVNVVQENDEILTKDSVVQRGEVLEDEQIKEKKAAFVTKNTVKTEDFNFSSRYVTDYK
                     NLENADSSKEKVYKNIEKLLPFYNSE"
BASE COUNT         6080 a         3538 c         4387 g         5433 t
ORIGIN      
        1 aagtaaaaac gtattgtcta ttggaaaaga aaaaagcttc tttcttttct acaattttat
       61 tctcaaatta tggtaaaatg gagagtaaga tttttattag aaatgagaca gatttgtatg
      121 agaaaattta atagccattc gattccgatt cggcttaatt tattgttttc aattgtcatt
      181 ttactttttc tgaccattat tggtcgtttg ttgtatatgc aggttctgaa taaggatttt
      241 tatgaaaaaa agctagcttc agctagtcag accaaggtta caaccagttc cgctcgtggg
      301 gagatttatg atgctagtgg caaacctttg gtagaaaata ctttaaagca ggttgtttcc
      361 tttacgcgta gcaataaaat gacggctaaa gatctgaaag aaatcgctag tcagttactg
      421 ggatatgtga gcatcacttc gccaaatttg acagaacgcc agctggcaga ttattattta
      481 gctgatcctg agatctataa aaaaacagta gaggctctcc caagtgagaa acgcttggat
      541 tcagatggta atcgtctatc tgaatcagaa ctgtataaca atgcggtcga tagtgtccag
      601 acgagtcaac taaactatac agaggatgaa aagaaagaag tctatctttt tagtcagtta
      661 aatgcggttg gaaactttgc gacaggaacc attgcgacag atcctctaaa tgattctcag
      721 gtggctgtta ttgcctctat ttcaaaggag atgcctggca ttagtatttc tacttcttgg
      781 gatcgaaaga ttttggaaac ttccctttct tctatagtag ggagtgtatc cagtgaaaaa
      841 gctggtctcc cagcggaaga agcagaatcc tatcttaaaa aaggctattc tctaaatgac
      901 cgtgttggaa cctcctattt ggaaaagcaa tatgaagaga ccttacaagg aaaacgctcg
      961 gtaaaagaaa tccatctgga taaatatggc aatatggaaa gcgtggacac aattgaggaa
     1021 ggtagtaagg gaaacaatat caaactgacc attgatttgg ccttccaaga tagcgtggat
     1081 gctttgctga aaagttattt caattccgag ctaggaaatg gtggagccaa gtattctgag
     1141 ggtgtgtatg cagtcgccct taaccccaaa acaggtgctg ttttgtctat gtcaggactc
     1201 aaacatgacc tgaaaacggg agagttgact cctgattcct tgggaacggt aaccaatgtc
     1261 tttgtcccag gttcggttgt taaggccgct accatcagct caggttggga aaatggtgtt
     1321 ttatcaggaa accaaacctt aacagatcag cctattgttt tccaaggttc agctccaatt
     1381 tattcttggt ataaattggc atatggatct tttcctatta cagctgtgga agccttggag
     1441 tattcatcca atgcttacat ggttcaaacc gctcttggaa tcatgggcca gacctatcaa
     1501 ccaaatatgt ttgttggaac cagcaatttg gaaacagcta tgggaaaact tcgtgcgacc
     1561 tttggcgaat atggcttggg ggctgcgacc ggaattgacc taccagatga atctactgga
     1621 tttgttccca aagagtatag ctttgctaat tacatcacca atgcctttgg gcagtttgat
     1681 aactatacgc ccatgcagtt ggctcagtat gtagcaacta ttgcaaatga tggtgttcgt
     1741 gtggctcctc gtattgttga aggcatttat ggtaataatg ataagggagg actgggtgac
     1801 ttgattcagc aactgcaacc gacagagatg aataaggtca atatatccga ctccgatatg
     1861 agtatcttgc accaaggttt ttatcaggtt gctcatggga ctagcggatt gacaactgga
     1921 cgtgcctttt caaatggtgc cttggtatcc attagtggga aaacaggtac tgccgaaagt
     1981 tatgttgcag gtggtcaaga agccaacaat accaatgcgg tggcctatgc tccatctgat
     2041 aatcctcaaa tcgctgttgc agtggtcttt cctcataata ccaatctaac aaatggtgta
     2101 ggaccttcca ttgcgcgtga cattatcaat ctgtatcaaa aataccatcc aatgaactag
     2161 aaaggaaatt atgctttatc caacacccat tgccaagctg attgacagtt attctaagtt
     2221 accaggtatc gggattaaga cggctacgcg tctggccttt tatacgattg ggatgtctga
     2281 tgatgatgtc aatgaatttg caaaaaatct cctttctgct aagagagaat tgacctactg
     2341 ttctatttgt ggacgtttga cagatgatga tccttgctcc atttgtaccg atccgactcg
     2401 tgaccagaca acgattttgg tgctagagga tagtcgtgat gtggcggcca tggaaaatat
     2461 ccaagaatac catggactct atcatgtcct gcatggcctc atttccccta tgaatggtat
     2521 cagtccagac gatatcaatc tcaagagtct catgactcgt ctgatggata gtgaggtttc
     2581 agaagtaatc gtagcaacca atgccacagc agatggggaa gcgacttcca tgtatctttc
     2641 ccgtttactc aagccagctg ggatcaaggt tacgcgccta gcacgaggcc tcgctgtggg
     2701 agcagatatc gagtatgcgg acgaagtgac cctcttacgg gctattgaaa atcgaacaga
     2761 gttgtaagtg tagacaaatt tacgaactcc attcatttat aaaaaatcaa ggaggctgaa
     2821 aatcgttcct atcggcctct ttttgtatag tgtgataagt agtgtcaggt tcaagtttta
     2881 aaaaaacaag caaatatgat atactaaaga gcaagtattc tagtagaatt aggacaaata
     2941 atatgaaaca aacgattatt cttttatatg gtggacggag tgcggaacgt gaagtttctg
     3001 tcctttcagc ggaaagtgtc atgcgtgcgg tcaattatga ccgtttcaca gtcaagactt
     3061 tcttcatcag ccagtcaggt gactttatca aaacgcagga atttagccag actcctgggc
     3121 aagaggatcg tcttatgacc aatgaaacca ttgattggga taagaaagtt gcaccaagtg
     3181 ctatctacga agaaggggca gttgtctttc cagttcttca tggtccgatg ggagaagatg
     3241 gctctgttca aggcttcctc gaagttttga aaatgcctta tgttggttgc aatattttgt
     3301 catccagtct tgccatggac aaaatcacga ccaagcgtgt gttagaatct gctggcattg
     3361 ctcaagttcc ttatgtggct atcgttgaag gtgatgatgt gactgctaaa atcgctgaag
     3421 ttgaagaaaa attgacttat ccagtcttca caaaaccatc aaatatgggt tcaagtgtcg
     3481 gtatttctaa atctgaaaat caagaagaac tccgtcaagc tttgaaactt gccttccaat
     3541 atgacagccg tgtcttggtt gagcaaggag tgattgcccg tgaaatcgag gttggactct
     3601 tgggaaacta cgatgtcaaa agcacgctgc caggagaagt tgtcaaggat gttgccttct
     3661 acgactacga tgccaagtat attgacaaca agattaccat ggatatccca gctaaaatca
     3721 gtgatgatgt ggtagctgtc atgcgtcaaa atgcagaaac ggccttccgt gccattggtg
     3781 gtttaggtct atctcgttgc gatttctttt atacagataa gggagaaatc ttcctaaatg
     3841 agctcaatac catgccaggt ttcacccagt ggtcaatgta tccattactt tgggacaata
     3901 tgggaatcag ctacccagat ctaatcgagc gtttggttga ccttgctaag gaaagttttg
     3961 acaagcgcga agcgcatttg ctataaaaat gaaagaaggg gtaggagcca gaaccatcac
     4021 tgcaaggtga ctagagttct cgggcttcga ccctttttaa aggagtagaa atgaaattaa
     4081 ctatccacga agttgcccaa gctgttggag cgaaaaatga tgtcagcatc tttgaggaca
     4141 tccagttaga aaaggctgag tttgacagtc gtttgattga ggaaggggac ttgtttgtgc
     4201 ccctcaaagg tgcgcgtgat ggtcatgact ttatcgaaat agcctttgaa aatggtgctt
     4261 ctgtaacctt gtctgagaaa gaaatcgcaa atcatcccta cattctagtt gatgatgttt
     4321 tgactgcctt tcaaactctc gcagcctact atcttgagaa aacggctgtt gatgtctttg
     4381 cagttacagg ttcaaatggt aagacgacga ccaaggatat gttggcgcac ttgctatcaa
     4441 caatctacaa aacctacaaa acacaaggaa actacaacaa cgagattggt cttccctaca
     4501 cagttctcca tatgcctgaa ggaacagaga agttggtctt ggagatgggg caggatcact
     4561 tgggagatat ccacctcttg tctgaattgg cgcatccaaa aacagccatt gtgaccttgg
     4621 ttggagaagc ccatttggcc tttttcaaag accgttcgga gattgcccaa ggaaaaatgc
     4681 aaattgctga tggcatggct tcgaattcct tgctcttgac gccagctgat cctattgtag
     4741 aggactactt gccaactgat aaaaaggtgg tccgttttgg gcaaggggca gagctggaag
     4801 tcacagactt gattgagcgt aaggatagtc tgacttttaa ggctaatttc ttaggacaag
     4861 cccttgattt gccagtgact ggtaagtaca atgccaccaa tgcaatgatt gcatcctatg
     4921 ttgccctaca agaaggagtt tcagaggagc aaattcatca ggccttccaa aatcttgaat
     4981 tgacgcgtaa ccgtaccgag tggaagaaag cagccaatgg tgcagatatt ctatcagatg
     5041 tgtataatgc caatccaact gctatgaagt tgattttaga gactttttcg gctattccag
     5101 ccaacgaagg tggcaaaaaa atcgctgtct tggcggacat gaaggaactc ggtgaccagt
     5161 ctgttcagct ccataaccag atgattttga gcctctcacc agatgtgctt gataccgtga
     5221 ttttctacgg agaagacatt gctgaattgg ctcaactggc cagtcaaatg ttcccaatcg
     5281 gccacgttta ctacttcaag aaaacagaat accaagacca atttgaagac ctagttaagc
     5341 aggtcaagga aagccttgga accaatgacc aaatcctgct caaaggctct aactctatga
     5401 atctagccaa gctggtagaa agtttagaaa atgaatgcta gtgattttgt caagtatttg
     5461 caaagaatga ttgccattac agatactggc ttaaccttta caaaagatcc ttttgaccgt
     5521 gagcgctacg aggacttgcg aagtctgtta tctgaaatgt tgaatcaggg atcagacctg
     5581 gatgtagaag aagtagcaga agtcttgaaa ccaacttcag cttatgcgac tcccttaatg
     5641 gacgtccgtg cttggattgt tgaggatgag aagatttgtc tggttagggg acaaggagag
     5701 gatagttggg ctttgccagg tggctttggt gaagtcggct attctccaac tgaaaatatt
     5761 ctcaaggaaa ttgaagaaga aaccggtttt aaagccaaag ttgaaagact gctagctgtt
     5821 tttgatacca atcgtttcca actacagagc aaacaatatg caaagtttgt ttttgaatgt
     5881 aagcttctcg atggacaatt ccaagaaaat caagaaatcg ctgaccttca attttttgca
     5941 attgaccaac tgccgaattt atctgaaaaa cgcattacca aggagcaaat cgagattctt
     6001 tggcaggttt atcaaggtca gagggagcaa tatcttgact aagaagatga ttatcgtatt
     6061 tctaaattca tttttcttaa gtagcatggt ataataaaat gcaggaaaat tttgaatcat
     6121 gaggaagact agatgaattt atgggatatt ttctttacga ctcaggcaac cgagccgccc
     6181 aaatttgacc ttttttggta tgttagccta tttacgctct tagccttaac cttttataca
     6241 gcccatcgct atcgtgaaaa gaaggtttac caacgatttt tccaaatctt gcagactgtt
     6301 cagttaatcc ttctttatgg ttggtactgg gtcaatcata tgccactgtc agaaagccta
     6361 cccttttacc attgccgtat ggctatgttt gtggtactct tgcttcctgg tcagtccaaa
     6421 tataaacaat actttgcatt attgggaaca tttgggacat tagcagcctt tgtttatcca
     6481 gtgccagatg cttacccttt tccacatatc accattctat cctttatctt tggtcattta
     6541 gcactcttgg ggaactctct agtttatcta ttgagacagt ataatgcgcg attgctggat
     6601 gtgaagggaa tttttctcat gacctttgcc ctaaatgcct tgatttttgt ggtcaatttg
     6661 gtgacaggtg gcgattacgg atttttgaca aaaccgccat tggttgggga tcacggtcta
     6721 gtagctaatt atttacttgt ttcaattgtg ctggtagcta ctatcagttt gactaagaaa
     6781 atcttagaat tctttttagc tcaagaagca gaaaaaatga ttgcaaagga agcttaacac
     6841 agagctttct tttttgctct tagagagttt ttacaagcag cttataaaat aagaatttct
     6901 gaatagacaa actcaaaaaa tggctgggaa atttaggaaa aaagcaagca cgattaaatt
     6961 ttttgtgtta taatattttg tgaatagcta tgcctatgtt tagctatgga ataatacgaa
     7021 gtgcgaaact tggaagatag agaggaagcg atgtaatggc tagagaaggc ttttttacag
     7081 gtctagatat tggaacaagc tctgtcaagg tgcttgtggc cgagcagaga aatggtgaat
     7141 taaatgtaat tggcgtgagt aatgccaaaa gtaaaggtgt aaaggatgga attattgttg
     7201 atattgatgc agcagcaact gctatcaagt cagccatttc ccaagcggaa gaaaaggcag
     7261 gcatttcgat taaatcagtg aatgtcggct tgcctggtaa tcttttgcag gtagaaccaa
     7321 ctcaggggat gattccagta acatctgata ctaaggaaat tacggatcaa gatgttgaaa
     7381 atgttgtcaa atcagctttg acaaagagta tgacacctga ccgtgaagtc attaccttta
     7441 ttcctgaaga atttattgtg gatggtttcc aagggattcg tgacccacgt ggcatgatgg
     7501 gggttcgcct tgaaatgcgt ggtttgcttt atacaggacc tcgtactatc ttgcacaatt
     7561 tgcgtaagac ggttgagcgt gcaggtgttc aggttgaaaa tgttatcatt tcaccactag
     7621 caatggttca gtctgttttg aacgaagggg aacgtgaatt tggtgctaca gtgattgata
     7681 tgggggcagg tcaaacgact gtcgctacaa tccgtaatca agaactccag ttcacacata
     7741 ttctccaaga aggtggagat tatgtaacta aagatatctc caaggttttg aaaacctctc
     7801 gcaaattagc ggaaggcttg aaactgaatt acggggaagc ctatccgcct cttgcaagca
     7861 aagaaacctt ccaagtagag gttattggag aagtagaagc agtcgaagtg acggaagcct
     7921 acttgtcaga aattatttct gcacgaatca agcacatcct tgaacaaatc aagcaagaat
     7981 tagatagaag gcgtctattg gacctccctg gtggtattgt cttaatcggt gggaatgcca
     8041 ttttaccagg tatggttgag cttgctcagg aagtctttgg cgtccgtgtc aagctttatg
     8101 ttccaaatca agttggtatc cgtaatccag cctttgcgca tgtgattagt ttatcagaat
     8161 ttgcgggtca attaacagaa gttaatcttt tggctcaggg agcgataaaa ggtgagaatg
     8221 acttaagtca tcagccaatt agttttggtg ggatgctgca aaaaacagct cagtttgtac
     8281 aatcaacgcc tgttcaacca gctcctgctc cagaagtaga gccggtggcg cctacagaac
     8341 caatggcgga tttccaacaa gcttcacaaa ataaaccgaa attagcagat cgtttccgtg
     8401 gattgatcgg aagcatgttt gacgaataaa gaggaaaaat aaattatgac attttcattt
     8461 gatacagctg ctgctcaagg ggcagtgatt aaagtaattg gtgtcggtgg aggtggtggc
     8521 aatgccatca accgtatggt cgacgaaggt gttacaggcg tagaatttat cgcagcaaac
     8581 acagatgtac aagcattgag tagtacaaaa gctgagactg ttattcagtt gggacctaaa
     8641 ttgactcgtg gtttgggtgc aggaggtcaa cctgaggttg gtcgtaaagc cgctgaagaa
     8701 agcgaagaaa cactgacgga agctattagt ggtgccgata tggtcttcat cactgctggt
     8761 atgggaggag gctctggaac tggagctgct cctgttattg ctcgtatcgc caaagattta
     8821 ggtgcgctta cagttggtgt tgtaacacgt ccctttggtt ttgaaggaag taagcgtgga
     8881 caatttgctg tagaaggaat caatcaactt cgtgagcatg tagacactct attgattatc
     8941 tcaaacaaca atttgcttga aattgttgat aagaaaacac cgcttttgga ggctcttagc
     9001 gaagcggata acgttcttcg tcaaggtgtt caagggatta ccgatttgat taccaatcca
     9061 ggattgatta accttgactt tgccgatgtg aaaacggtaa tggcaaacaa agggaatgct
     9121 cttatgggta ttggtatcgg tagtggagaa gaacgtgtgg tagaagcggc acgtaaggca
     9181 atctattcac cacttcttga aacaactatt gacggtgctg aggatgttat cgtcaacgtt
     9241 actggtggtc ttgacttaac cttgattgag gcagaagagg cttcacaaat tgtgaaccag
     9301 gcagcaggtc aaggagtgaa catctggctc ggtacttcaa ttgatgaaag tatgcgtgat
     9361 gaaattcgtg taacagttgt tgcaacgggt gttcgtcaag accgcgtaga aaaggttgtg
     9421 gctccacaag ctagatctgc tactaactac cgtgagacag tgaaaccagc tcattcacat
     9481 ggctttgatc gtcattttga tatggcagaa acagttgaat tgccaaaaca aaatccacgt
     9541 cgtttggaac caactcaggt atctgctttt ggtgattggg atcttcgccg tgaatcgatt
     9601 gttcgtacaa cagattcagt cgtttctcca gtcgagcgct ttgaagcccc aatttcacaa
     9661 gatgaagatg aattggatac acctccattt ttcaaaaatc gttaagtaaa tgaatgtaaa
     9721 agaaaataca gaacttgttt ttcgagaagt tgcagaggct agtctgagtg ctcatcgaga
     9781 gagtggttcg gtctctgtca ttgcagttac caagtatgta gatgtaccga cagcggaagc
     9841 cttgcttccg ctaggtgttc atcatatcgg tgaaaatcgt gtagataagt ttctggaaaa
     9901 atatgaagct ttaaaagatc gagatgtgac ttggcatttg attggtacct tgcaaagacg
     9961 taaggtgaaa gatatcattc aatacgttga ttatttccat gcattggact cagtaaagct
    10021 agcaggggaa attcaaaaaa gaagtgaccg agtcatcaag tgtttccttc aagtaaatat
    10081 ttctaaagaa gaaagcaaac acggtttttc gagagaggaa ctgctggaaa tcttgccaga
    10141 gttagccaga ctagataaga ttgaatatgt tggtttaatg acgatggcac cttttgaggc
    10201 tagcagtgag cagttgaaag agattttcaa ggcgacccaa gatttacaaa gagaaattca
    10261 agagaaacaa attccaaata tgcctatgac cgagttaagt atgggaatga gtcgtgatta
    10321 taaagaagcg attcaattcg gttccacttt tgttcgtata ggtacatcat tttttaagta
    10381 ggagagaacc atgtctttaa aagatagatt cgatagattt atagattatt ttacggagga
    10441 tgaggattca agtctccctt atgaaaaaag agatgagcct gtgtttactc cagtaaattc
    10501 ttcacaggaa ccggctctcc caatgaatca accttcacag tcggctggta caaaagagaa
    10561 caatatcacc agacttcatg caagacaaca ggaattggca aatcagagtc agcgtgcaac
    10621 ggataaggtc attatagatg ttcgttatcc tagaaaatat gaggatgcaa cagaaattgt
    10681 tgatttattg gcaggaaacg aaagtatctt gattgatttt cagtatatga cagaggtgca
    10741 ggctcgtcgt tgtttggact atttggatgg agcttgtcat gttttagctg gaaatttgaa
    10801 aaaggtagct tctaccatgt atttgttgac accagtgaac gttattgtaa atgttgaaga
    10861 tatccgttta ccagatgaag atcaacaggg tgagttcggt tttgatatga agcgaaatag
    10921 agtacgataa tgattttttt aattcgtatg atttataatg cagtggatat ttactccctg
    10981 attttggtag ccttcgctgt catgtcttgg tttccaggtg cctatgaatc cagtttaggt
    11041 cgttggattg tagcgttggt aaaaccagtg cttgctccct tgcaacgcct gcctttacag
    11101 atagcgggtc ttgatttatc tgtttgggtt gcgattgttt tggttcgatt tttaggagaa
    11161 aacctagtgc gttttctggc gatgatagga tgaataaagg gatttatcag catttctcca
    11221 tagaagatcg tccatttctt gacaagggaa tggaatggat aaagaaggta gaagatagct
    11281 atgctccttt tttaactcct tttatcaatc ctcatcagga gaagctatta aagattttgg
    11341 ccaaaaccta tggtcttgct tgtagcagta gtggggaatt tgtctcgagt gagtatgttc
    11401 gagttttatt atacccagat tatttccaac cagagttttc agattttgaa atatctcttc
    11461 aggaaattgt gtattccaat aaatttgaac atttaacgca tgctaagatt ttagggacag
    11521 tcatcaatca attagggatt gaacggaaac tttttggaga tatcctagta gatgaagagc
    11581 gggcgcagat tatgattaat cagcagtttc ttcttctctt tcaagatgga ttaaagaaaa
    11641 ttggtcgtat acctgtttcg ctggaggaac gtcctctcgc cgagaaaata gataagctag
    11701 aacagtatcg agaactggat ttatctgtgt ctagttttcg attagatgtt cttttatcaa
    11761 atgttttgaa actatctagg aatcaagcaa accagttgat tgaaaagaaa cttgtccaag
    11821 taaattatca tgtggtagac aaatcagatt atactgttca agttggagat ttgattagtg
    11881 tgagaaaatt tggtcgcttg aaattacttc aagataaggg acaaacgaaa aaagagaaga
    11941 aaaaaataac cgtccagtta ttattaagta agtgaggaat agaatgccaa ttacatcatt
    12001 agaaataaag gacaaaacct ttggaactcg attcagaggt tttgatccag aagaagtcga
    12061 tgaattttta gatattgtgg ttcgtgatta cgaagatctt gttcgtgcaa atcatgataa
    12121 agatttgcgt attaagagtt tagaagagcg tttgtcttac tttgacgaaa tgaaagattc
    12181 attgagccag tctgtattaa ttgcccagga tacagcggag agagtgaaac aggcagcgca
    12241 tgaacgttca aataatatca ttcatcaagc agagcaagat gcccagcgct tgttggaaga
    12301 agctaagtac aaggcaaacg agattcttcg tcaagcaact gataatgcta agaaagttgc
    12361 tgttgaaaca gaagaattga agaacaagag ccgtgtcttc caccaacgtc tcaaatctac
    12421 aattgagagt cagttggcta ttgttgaatc ttcagattgg gaagatattc ttcgcccaac
    12481 agctatttat cttcaaacca gtgatgaagc ctttaaagaa gtggttagcg aggtcttggg
    12541 tgagtctgtg ttacagcaac atccagagga agaaccaatt gatatgacac gtcagttctc
    12601 tcaagtagaa atggcagaat tacaggctcg tattgaggca gccaataaag aattggctga
    12661 atttgaagct cagactaaac aggaagtgga agctccaact cctgtagtga gtcctcaaat
    12721 tgaagaagaa cctactcaac cagttggtcc aatgtatgaa gaaccagaag tagctccagt
    12781 atatccttca gcttcaacac cagctacaga aactgttgat tcagcaccag gatttgaagc
    12841 accgcaagaa tctgttacaa ttttataaga aatattctga gaacaatatc ttatccttat
    12901 atttccagcg agcaggagat ggtgtgagtc ctgtaatccc taacgataag attatcctct
    12961 caaaaactca agtctgaagt tagtaggatt tgacgttccc cacgttacgg gataagaggg
    13021 agaaagacta gatctttttc cgaataaagg tggtaccacg attttcgtcc tttttggaag
    13081 tcgtggtttt taatttgtta ttatttataa aggagatacc atgaaactca aagataccct
    13141 taatcttggg aaaacagctt ttccaatgcg tgcaggcctt cctaccaaag agccagtttg
    13201 gcaaaaggaa tgggaagatg caaaacttta tcaacgtcgt caagaactaa atgaagggaa
    13261 accgcatttt gtcttgcatg atggccctcc atacgcaaac ggaaatatcc acgttggaca
    13321 tgccatgaat catatctcaa aagatatcat cattcgttct aagtctatgt caggttttaa
    13381 ctcaccttat atcccgggtt gggatactca tggtctgcca atcgagcaag ttttggcaaa
    13441 gcaaggtgtt aagcgcaaag aaatggactt ggttgagtac ttgaaacttt gtcgtgagta
    13501 cgctctttct caagtttata aacaacgtga tgatttcaag cgcttgggga tgtcaggtga
    13561 ctgggaaaat ttatatgtga ctttaactcc agactacgaa gcagcccaaa tccgtgtatt
    13621 tggtgagatg gctaataaag gctatatcta ccgtggtgca aaaccagttt actggtcttg
    13681 gtcatctgag tcagcccttg ctgaagcgga aattgaatac catgatttgg tttcaacttc
    13741 cctttactat gccaataagg taaaagatgg caaaggtgtt ctagatacag atacttatat
    13801 cgttgtttgg acaactactc catttactat tacagcttct cgtggtttga ctgttggtgc
    13861 agatattgat tacgttttgg ttcaacctgc tggtgaaact cgtaagtttg tggttgctgc
    13921 agaattgttg attagcttgt ctgagaaatt tggttgggct gatgttcaag tgttggcaac
    13981 ttaccgtggt caagaactca accacatcgt aacagaacac ccatgggata cagttgtaga
    14041 tgagttggtt atccttggtg accacgttac aactgactct ggtactggta ttgtccatac
    14101 agcccctggt tttggtgagg atgactacaa tgttggtatt gctaatggtc ttgaagtcgc
    14161 agtgactgtt gacgaacgtg gtatcatgat gaagaatgct ggtcctgagt ttgaaggtca
    14221 attctatgaa aaggtggttc caactgttat tgaaaaactt ggtaacctcc ttcttgctca
    14281 agaagaaatc tctcactcat acccatttga ctggcgtacc aagaaaccaa ttatctggcg
    14341 tgcagtacca cagtggttcg cctctgtttc aaaattccgc caagaaatct tggatgctat
    14401 tgataaagtt aaattccata cagaatgggg gaaagtacgt ctatataata tgattcgtga
    14461 ccgtggtgac tgggttatct ctcgtcaacg tgcttggggt gttccacttc caatcttcta
    14521 tgcagaagac ggtacagcta tcatgacagc tgaaacgatt gaacacgtag ctcaactctt
    14581 tgaagtacat ggttcaagca tttggtggga acgtgatacc aaagacctct tgccagaagg
    14641 atttacacat ccaggttctc caaatgggga gttcaagaaa gaaacagata ttatggatgt
    14701 atggtttgac tcaggttcat catggaatgg tgttttggta aatcgtccaa atctgactta
    14761 tccagctgac ctttaccttg aaggttctga ccaataccgt ggttggttca actcatcact
    14821 cattacatct gttgccaacc atggcgtagc accttacaaa caaatcttgt cacaaggttt
    14881 tgcccttgat ggtaaaggtg agaagatgtc taaatctctt ggaaatacca ttgctccaag
    14941 cgatgttgaa aaacaatttg gtgctgaaat cttgcgtctc tgggtaacaa gtgttgactc
    15001 aagcaacgac gtacgtatct ctatggatat cttgagccaa gtctctgaaa cttaccgtaa
    15061 aatccgtaac acccttcgtt tcttgattgc caatacatct gactttaacc cagctcaaga
    15121 cgcagtagca tacgaagagc ttcgctcagt tgataagtac atgactattc gctttaacca
    15181 gcttgtcaag accattcgtg atgcttacgc aaactttgaa ttcttgacaa tctacaaggc
    15241 cttggtgaac tttatcaacg ttgacttgtc agccttttac cttgattttg ctaaggacgt
    15301 tgtttacatc gaaggtgcta aatcattgga acgccgtcaa atgcagactg tcttctatga
    15361 cattcttgtg aaaatcacga aactcttgac tccaatcctt cctcacactg cggaagaaat
    15421 ctggtcatat cttgagtttg aagccgaaga cttcgttcaa ttgtcagaat tgccagaagc
    15481 ggaaactttt gctaatcaag aagaaatctt ggatacatgg gcagccttca tggacttccg
    15541 tggacaagct caaaaagcct tggaagaagc tcgtaatgca aaagtaatcg gtaaatcact
    15601 tgaagcacac ttgacagttt atccaaacga agttgtgaaa actctactcg aagcagtaaa
    15661 cagcaatgtg gcccaacttt tgatcgtgtc tgagttgacc atggcagagg gaccagctcc
    15721 ggaagctgcc cttagcttcg aagatgtagc cttcacagtt gaacgtgctg ctggtgaagt
    15781 atgtgaccgt tgccgtcgta tcgacccaac aacagcagaa cgcagctacc aggcagttat
    15841 ctgtgaccac tgtgcaagca tcgtagaaga aaattttgcg gaagcagtcg caggaggatt
    15901 tgaagagaaa taagatgaaa agtctctatg cgtctagtat ataaaaatga gttattccaa
    15961 gtttataatc tctcttgtga gatgctaaag tttgatgaga attggttttg ctaatattag
    16021 gagaaagtaa gtatgctaaa attgcaagat tgtttttctt gcgattgttt aaactagtga
    16081 ggaaaaataa acattatgag acaaacaaat gttagaggaa gcttgtccct ccgtggcttt
    16141 agaaaattaa gaactggtgc aattgttgcg acaggtgtga ttctactggg gctgggcttt
    16201 tcagctccaa ctgcgtcagt cagtgaacaa gatggtgttt gggtagccag aacagttgca
    16261 gaagtaaaag ctgatattca aaaagaaggt gatttattta agtatgtcat taagtgggga
    16321 gatacattaa gtgttatcag cgaagcaagc aatatccctg tagatgtact ccaagaaatg
    16381 aatcaagctt caggcaagaa tttattctta ccatacaatg aattgtactt tactgaagct
    16441 gaaacagttt cagatacagt cacaaaacac aagattgtca tcaaagaaag tggtgcaagt
    16501 ggcgaagttc gcacttatga ggtcttgctc aagaaagacc aagaaacaaa agctgttacc
    16561 tttgaattaa cagatgtcac tgagaaagtt gaaaaaatga ctgtgaacta tagcccagtt
    16621 tatcagcctg tatcagtacc agtagtggaa agagcggaag aatcagttga agagcctgcc
    16681 ctagtagcac cagctgtgac aagcccagaa gttaaagaag aaacagaaga tccagtagta
    16741 ctaaaagcag aagaagcagc gactacccca gcaacgaaag aaacagcacc agaggtggta
    16801 acaccagctg aaccaaacgc agaaacccca gcgccgaaag tagaagcgcc aacagaatca
    16861 tctgaaccaa aaggaacaga gccagaggta gccaaaccag ctggagattc agtatcatct
    16921 acaagtgagt caaatgagga tggcaattca ggcgattcaa ctcattcaac attgacctct
    16981 gatgaaacaa aaccagaaga ggtagaatca acttcagtaa cggacaatgt cccttcaaat
    17041 gcagggacaa caaatcaacc tctaacccca acatctaatt ctgatgattc agcaccaaaa
    17101 gaagtagcaa atggaactga aggaaaagta gaagcgccga aagttgaaga agcagtatca
    17161 gaaggagaag aaggtaacgc cgaaataaca aataatccat caacaggcgc tactccttca
    17221 agtgatgaaa cgacagaaca atctggaact ccagctccaa aaccagaaga ggaagctcca
    17281 gtagcgacag aaacctcagc ggcatcagct gaatcaacag aatcagcacc agtggtagca
    17341 aaaccagctg agccaaaagc agaaacacca gaagcaacaa ttacaggaga tgaaggaaaa
    17401 gaatctgaag aatcaggaac atctgcaact gtggaatcaa caccagcagg agaagaagga
    17461 aaagcggagg attcagtttc ttcaactgaa tcttcatcaa ctgcttctga aggggagtca
    17521 acagctacgc aaccagctgt agaagcagga acagattcaa catcggaagc tgaagaactt
    17581 gcaactacgg aaacagcaac aacgccgaaa acggaagagc cagaagcggt agccccagct
    17641 ccagaagcac cctcagaatc agactcgaaa gaagaagatt cagctgcagg ggatacaaca
    17701 gttacagaag aacagccagc tgcagaaact gtctctgaag gtggatcacc agctacgcaa
    17761 ccggctggag aagaaggtaa atctgaaaaa gaaccgaaag tagaagggca accatcaggt
    17821 acagagtcgg caaccccaga acagcataca aatattgagg aaacaacaga aaagcaatcg
    17881 ggggctacat cagaaaattc aggtgatagt ctagtatcag atgagccagc tgcgagctca
    17941 ggtatttcaa catcttcagg aacagaaggg gcagaaacga tagatacaga aacaattgca
    18001 gaaaaatcag aaataccgtc tccagcagaa gaaggatctg ctaaagaaga aaaagaagtt
    18061 attaagaaac tagaacttag aaatatatct tctgttgaac tatattctaa agaaggtgat
    18121 aagtatcgtc atgtaacttc acttgctacc ttaccaagta atccagagac ttactttatg
    18181 aaagttaaat cagagaactt taaagatgtt atgctaccag ttacaagcat tactaatgat
    18241 aataaagata atagagatgt ttataaaata gtagcaagtg taaagaatct gatacagcat
    18301 gaaaataata aagtactaga gaattataca tactatttat caaaaactca gcaaggtgaa
    18361 actggagtat atacatcatt taaaaattta gttgatgcga tgaatagaga ttcttatgga
    18421 gagtttcgtt taggtgcaac tatggatgct cgtgaagttg aacttccaga tggtcaagaa
    18481 agttatgtga agaatgtatt tcacggtaga ctaataggtc aaaatagtaa taagtattat
    18541 gctatatata atttgaaaaa gccattattt aatgctttaa gtaatgctag agtacaaaat
    18601 ctttcactaa aagatgttaa tatttccgca aaggacgata ctgcaacact agcgaaagaa
    18661 gcaaataaca atactcatat tgataatgtt cattctgatg gagcgatagc cggggaacgt
    18721 agcataggag gattagtatc acaagttaat aattctacta tttctaatag tagctatact
    18781 ggtagaataa ctaatacata taaaacagtt gctagttatc aaatcggagg tttagtaggt
    18841 aaactttctg gtccgaatgg tttaattgat aaatcgatag catcaataga tttggcttca
    18901 aacgcaacac gaggtgatca gtctattggt ggaatagctg gttcagtaat tgataatgca
    18961 gtgataagta gtagttatgc tgaagggaaa ttaaataatg ttcaaccttt tgcaaatgtt
    19021 ggtggagtag ttggtgacct ttgggatcca gtaggcggat tggaaaaatc tggtcaacta
    19081 tctaatgtac taagtgatgt taatgttaca aacggtaatg ctatcgcggg taaacatttt
    19141 gatcacatga aagcaactaa tgtttatagt aataaaaata ataaggttgt gaacgtagta
    19201 caagaaaatg atgaaatttt aactaaggat tctgttgttc aaagaggaga agtattagaa
    19261 gatgaacaaa taaaagagaa aaaagctgct tttgttacta aaaatactgt taaaacagaa
    19321 gattttaatt tctcttcaag atatgtaact gactataaaa atcttgagaa tgctgattcg
    19381 tcaaaagaaa aagtatataa aaatattgaa aaactattac cattttataa tagcgaaa
//
LOCUS       DAVKDZ010000035        19336 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000035 DAVKDZ010000000
VERSION     DAVKDZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19336
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.036"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            3..75
                     /locus_tag="VWZ30_001218"
     tRNA            3..75
                     /locus_tag="VWZ30_001218"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Val,seq:tac)
     gene            78..150
                     /locus_tag="VWZ30_001219"
     tRNA            78..150
                     /locus_tag="VWZ30_001219"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Asp,seq:gtc)
     gene            175..247
                     /locus_tag="VWZ30_001220"
     tRNA            175..247
                     /locus_tag="VWZ30_001220"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:208..210,aa:Lys,seq:ttt)
     gene            253..334
                     /locus_tag="VWZ30_001221"
     tRNA            253..334
                     /locus_tag="VWZ30_001221"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:287..289,aa:Leu,seq:tag)
     gene            353..425
                     /locus_tag="VWZ30_001222"
     tRNA            353..425
                     /locus_tag="VWZ30_001222"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:386..388,aa:Thr,seq:tgt)
     gene            448..519
                     /locus_tag="VWZ30_001223"
     tRNA            448..519
                     /locus_tag="VWZ30_001223"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:480..482,aa:Gly,seq:gcc)
     gene            527..612
                     /locus_tag="VWZ30_001224"
     tRNA            527..612
                     /locus_tag="VWZ30_001224"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:561..563,aa:Leu,seq:taa)
     gene            622..695
                     /locus_tag="VWZ30_001225"
     tRNA            622..695
                     /locus_tag="VWZ30_001225"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:656..658,aa:Arg,seq:acg)
     gene            711..784
                     /locus_tag="VWZ30_001226"
     tRNA            711..784
                     /locus_tag="VWZ30_001226"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:745..747,aa:Pro,seq:tgg)
     gene            790..863
                     /locus_tag="VWZ30_001227"
     tRNA            790..863
                     /locus_tag="VWZ30_001227"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:824..826,aa:Met,seq:cat)
     gene            877..950
                     /locus_tag="VWZ30_001228"
     tRNA            877..950
                     /locus_tag="VWZ30_001228"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:911..913,aa:Ile,seq:cat)
     gene            961..1050
                     /locus_tag="VWZ30_001229"
     tRNA            961..1050
                     /locus_tag="VWZ30_001229"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:997..999,aa:Ser,seq:tga)
     gene            1061..1134
                     /locus_tag="VWZ30_001230"
     tRNA            1061..1134
                     /locus_tag="VWZ30_001230"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1095..1097,aa:Met,seq:cat)
     gene            1138..1210
                     /locus_tag="VWZ30_001231"
     tRNA            1138..1210
                     /locus_tag="VWZ30_001231"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1171..1173,aa:Phe,seq:gaa)
     gene            1230..1300
                     /locus_tag="VWZ30_001232"
     tRNA            1230..1300
                     /locus_tag="VWZ30_001232"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1262..1264,aa:Gly,seq:tcc)
     gene            1336..1409
                     /locus_tag="VWZ30_001233"
     tRNA            1336..1409
                     /locus_tag="VWZ30_001233"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1370..1372,aa:Ile,seq:gat)
     gene            1416..1503
                     /locus_tag="VWZ30_001234"
     tRNA            1416..1503
                     /locus_tag="VWZ30_001234"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1450..1452,aa:Ser,seq:gct)
     gene            complement(1629..2564)
                     /gene="birA"
                     /locus_tag="VWZ30_001235"
     CDS             complement(1629..2564)
                     /gene="birA"
                     /locus_tag="VWZ30_001235"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HEU9334970.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDIDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRTFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(2561..3421)
                     /locus_tag="VWZ30_001236"
     CDS             complement(2561..3421)
                     /locus_tag="VWZ30_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9334971.1"
                     /translation="MLVFSEYQTGTINLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKAPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            3529..5691
                     /locus_tag="VWZ30_001237"
     CDS             3529..5691
                     /locus_tag="VWZ30_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU9334972.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVIDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVKKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIILSEFSKKERPILINNWEATY
                     FDFQREKLLELADESKKVDIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNIDAIARLPIQYGSSYLYPTISMGD
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVSLSQGDFLSRQYIFRKL"
     gene            5783..7042
                     /locus_tag="VWZ30_001238"
     CDS             5783..7042
                     /locus_tag="VWZ30_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9334973.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGVGDLAWSISATTKHPKEANAFVEYMTRPE
                     VIQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            7056..7922
                     /locus_tag="VWZ30_001239"
     CDS             7056..7922
                     /locus_tag="VWZ30_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9334974.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            7936..8769
                     /locus_tag="VWZ30_001240"
     CDS             7936..8769
                     /locus_tag="VWZ30_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9334975.1"
                     /translation="MKQDGRKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNFVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            9081..10523
                     /gene="gtfA"
                     /locus_tag="VWZ30_001241"
     CDS             9081..10523
                     /gene="gtfA"
                     /locus_tag="VWZ30_001241"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU9334976.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYVEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPIQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYYFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            10661..11854
                     /locus_tag="VWZ30_001242"
     CDS             10661..11854
                     /locus_tag="VWZ30_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU9334977.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            12541..14520
                     /locus_tag="VWZ30_001243"
     CDS             12541..14520
                     /locus_tag="VWZ30_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9334978.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAARETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            14587..16083
                     /locus_tag="VWZ30_001244"
     CDS             14587..16083
                     /locus_tag="VWZ30_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9334979.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            16083..17009
                     /locus_tag="VWZ30_001245"
     CDS             16083..17009
                     /locus_tag="VWZ30_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9334980.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            17018..18085
                     /locus_tag="VWZ30_001246"
     CDS             17018..18085
                     /locus_tag="VWZ30_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9334981.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKICEKMGFAHLAD"
     gene            18096..19022
                     /locus_tag="VWZ30_001247"
     CDS             18096..19022
                     /locus_tag="VWZ30_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9334982.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            complement(19097..>19336)
                     /locus_tag="VWZ30_001248"
     CDS             complement(19097..>19336)
                     /locus_tag="VWZ30_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9334983.1"
                     /translation="KQVHPLFQTVFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLI
                     KRNAFGFRNFENFRKRIFIALNIKKERTKFVLSRA"
BASE COUNT         5569 a         3497 c         4174 g         6096 t
ORIGIN      
        1 tagggagttt agctcagctg ggagagcatc tgccttacaa gcagagggtc agcggttcga
       61 tcccgttaac tcccaaaggt cccgtagtgt agcggttatc acgtcgccct gtcacggcga
      121 agatcgcggg ttcgattccc gtcgggaccg tttaaggtaa cgcaagttat tttagactcg
      181 ttagctcagt tggtagagca attgactttt aatcaatggg tcactggttc gagcccagta
      241 cgggtcatat atgcgggttt ggcggaattg gcagacgcac cagatttagg atctggcgct
      301 taacggcgtg ggggttcaag tcccttaacc cgcattaaga tataacaaat gagccggctt
      361 agctcagttg gtagagcatc tgatttgtaa tcagagggtc gcgtgttcaa gtcatgtagc
      421 cggcattttt agatagaaga aaacagtgcg aacgtagttc agtggtagaa caccaccttg
      481 ccaaggtggg ggtcgcgggt tcgaatcccg tcgttcgctt agagaggccg gggtggcgga
      541 actggcagac gcacaggact taaaatcctg cgattggtaa cgatcgtacc ggttcgattc
      601 cggtcctcgg cataatatgg agcaccctta gctcaactgg atagagtacc tgactacgaa
      661 tcaggcggtt agaggttcga ctcctctagg gtgcattttt ctatttaact cgggaagtag
      721 ctcagcttgg tagagtactt ggtttgggac caaggtgtcg caggttcgaa tcctgtcttc
      781 ccgattcatg gcggtgtagc tcagctggct agagcgtccg gttcataccc gggaggtcgg
      841 gggttcgatc cccttcgccg ctataattat cttgtcggac ctttagctca gctggttaga
      901 gctctcggct cataaccgag tggtcgtagg ttcaagtcct acaaggtcca tttgaatatt
      961 ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa aaccgtcagg cgtgtaaaag
     1021 cgtgcgtggg ttcgaatccc acatcctcct tttatattaa cgcgggatgg agcagctcgg
     1081 tagctcgtcg ggctcataac ccgaaggtcg taggttcaaa tcctgctccc gcaataaggc
     1141 tcggtagctc agttggtaga gcaatggatt gaagctccat gtgtcggcgg ttcgattccg
     1201 tctcgcgcca ttttattgat agtatttatg cgggtgtagt ttagtggtaa aactacagcc
     1261 ttccaagctg ttgtcgcgag ttcgattctc gtcacccgct ttgaactttg ttcaaattac
     1321 caagtttttt aacttgggcg cgtagctcag gtggttagag cgcacgcctg ataagcgtga
     1381 ggtcggtggt tcgagtccac tcgtgcccat taattggaga attactcaag aggctgaaga
     1441 ggacggtttg ctaaatcgtt aggtcgggta actggcgcaa gggttcgaat cccttattct
     1501 ccgttttaat gagagtttgt aactctcttt tttattttta gagacaataa aacttcagat
     1561 ccgtaaaact taattgaatt actaatctga agttattttt atatcgttta tattataatt
     1621 gtgttatttt acttccaact attgagagaa atttcgccac tatttagcca gatttctttt
     1681 ccgttatcac attgaactaa aagttttcca ttttctgaga tgtctttagc aagtcccttg
     1741 tagtcttttt gctctagtgt gaaagtgact tcttttccta gaatgaatga ctgttttttg
     1801 tataggtata atagctcttc tgctggtgtt tcgaagaaag tacgccagat ttctatgatc
     1861 aattcattcc ttgttatagg agctgtagct ttaaataagc tggcagcttt ttcttttaat
     1921 tcctgaggga agtctttaat agtgaaattg atacctactc caataatgat atctgtgact
     1981 aagccagttt ctacagaggt cattgcttca gtaaggattc ctccaatttt atgattgttt
     2041 agatagatat cattgaccca ttttatgtcg atatctatta aagttaggtt cttaatggct
     2101 ttgtagacag ctccagctac aagtagtgtg taggatggta atttgtcata ggggagattt
     2161 ggtttaagat ggagtgtcat ataaatacca ccttgtggtg agtagaagga acgttgaaaa
     2221 cggcctcggc ctgctgtttg ataggaagct agatagaggg tatttgcttc atggcctaaa
     2281 tcaattgctt cttttgcatc tagttgtgtt gattttgttt cgggtttaaa gctgacttta
     2341 attggaagat tttcttctag aatctctgga agaataaggt caccattcat cagtttgtat
     2401 cctctatttt tgatactatc aatttcaatg ccttcttgtt ctagtcgctt gatggctttc
     2461 caaattgatg ttcggcttag ggatagtttt tctgcgattt tttctccgct gatatagtcg
     2521 gtttctttag ataggatttg gtagacagct tggtaggatt tcataatgtt gcctttctca
     2581 ctagttggta ttgagagtat tcttttcttg tatgacttgg agtctgatta aagtattgtt
     2641 tataagcttt cgaaaaatgg agtggatctg aaaaacctac cgagtatgca attaccttga
     2701 tggactcttg ggtattttcg agaagttgtc tagctcggtg cattcgaacg tagagtaggt
     2761 attctttggg tgataaggta ttaaattctt tgaatacgct tgataagtag cttctgtgaa
     2821 cggatagttc ttttgctaaa tcttgaattg taagtgattg aggatagtgg ctatcaatta
     2881 atcgtttgca ttcaagatag agttggtggg ttgatgaaat attctttttt ttctgattgg
     2941 gagcaatagt tcccagatga aacatcagtt catgaagttg tcccatgata tggagttgag
     3001 ctaattcact tgattttgta atctgagcga agcggacaat gtctgagatg agttttgcag
     3061 tagtctgggt atgacaagtt tcagattgga tgagataaga ttgatcagaa atttgagaaa
     3121 gagcaaaata atcaggggct ttccctccag tgattcctaa ccagtagtag gcccaaggtt
     3181 ctttactatc tgcttgataa aaggttagtt cctctggttt taatagaaag aaatctcctt
     3241 cttttaaatc aacaatttta cccttgtaat gaaattttcc ttgtccttta gtaatgtaat
     3301 gtaggacgta tgtatcacga atggctggac caaaagagta attaggtgtg cattcctcat
     3361 atccataaaa gcttagggca aggttgattg ttccagtctg gtattctgaa aaaactaaca
     3421 tgtgctacct cctacctaac attttaccat attcttgaga catttttcta ttttggaaag
     3481 cgatttcagg tgataaaata taatcaataa agtgatgagg tgaagtaaat gggagttagg
     3541 atagagaata atctatttta tgttgagagt aaaaatctaa gtttgattat tgaaaatcga
     3601 aatggctact tacttttgaa acatttagga aagactatta agaactataa aggttccaat
     3661 agtgtttatg aacgagacca tgccttttca ggaaatccaa cggctactaa tcgaaccttt
     3721 agtttagata ctcagcgaca gatttttgga caacatggct taggagattt taggaaacca
     3781 accatacagg ttcagcatag tgtaactgaa gtaatagact ttcgatttgt agaagcaaag
     3841 attttaaaag gtcagaatgg tccacagggc ttaccttctc cacatagcat ggacgataca
     3901 gagactcttg tcttaatgtt agaagattct aaggctcaac ttagtctgac tttgtattat
     3961 actactttta ataatgatgc gactattgct agctacagta aattagataa taatagtaat
     4021 caggaagttg tcatccataa agatttttct tttatggctg attttccagc tgcagattac
     4081 gaaatagtaa ctctgcaggg tgcttatgct cgtgaaaaga ctgttagacg tcaacaggta
     4141 gaacaaggaa tcttttcgat tagttcaaac cgaggtgctt ctggtcatgc tcaaacacca
     4201 gctcttctac tatgcgaaca aggagtcaca gaggatgctg ggaatgtgtt tgctattcaa
     4261 ctaatgtata gtggcaactt tgaagctttt gttaaaaaaa atcaattgaa tgaagttcgg
     4321 gtggctattg gcattaatcc agaaaacttt tcttggaagt tagcccctga ggaatacttt
     4381 gaaacaccgg tagctttagt gactcattca gatcagggat taactggtat tagtcatgaa
     4441 agtcagaatt ttgtactgaa gcacattatt ctaagtgaat tttctaaaaa agaacgtcca
     4501 attctaatca ataactggga agctacttac tttgactttc agagagaaaa actgttagag
     4561 ttagcagatg aatctaagaa agttgacatt gaactttttg tattagatga tggttggttt
     4621 ggcaatcgtt ttgatgataa tcgtgcttta ggtgattggg ttgttaatga ggaaaaactg
     4681 ggtggaagtc tagaaagtct gatttcagct atccatgaaa gaggtttgca gtttggactt
     4741 tggttagaac ccgaaatgat ttctgtagat agtgatttgt atcgtcaaca tcctgactgg
     4801 gctattcagg ttcctgatta tgagcatact tattctcgga atcaattagt acttaatctt
     4861 gccaatcctc aggtagtaga atacttgaaa agtgtcttag atcaactcct atcttatcat
     4921 gagattgatt acattaaatg ggatatgaac cgcaatatca ctaagctagg gaatggatta
     4981 acttatctag agacacagat gcaatctcat cagtacatgc tggggcttta cgaactcgtt
     5041 tcttatctga cagagaagca cagccatatt ctctttgagt cctgctctgg tggtggtgga
     5101 cgaaatgatc ttggtatgat acgctatttc ccacaagtct gggctagtga taatattgat
     5161 gccattgcac gtttaccaat tcaatacggt tcatcctatc tctatccaac catttctatg
     5221 ggggatcatg tgtcagcagt accgaatcat cagatgggac gaatgacacc attagaaaca
     5281 cgtggccatg tagcaatgat gggaaatttg ggctatgagc ttgatttgac aaatttatca
     5341 gatgaagaga aagctacgat tgctaatcag gtgaacttgt ataaagaatt acgaccagta
     5401 gttcagttag gacagcagta tagactaatt aatcctgata ctgcatccaa tgaagcagct
     5461 gtacaattta attacggaaa tcaaacgatt gtaacctacg ttcgcgtttt atctgttgta
     5521 gagaccatgg aaacaacttt aaaattaaaa gatttggatg aagagggact atataaatta
     5581 caggaaaatg gcgaagttta ctcaggtgca gaactaatgt atgcgggctt aactgttagc
     5641 ttatcccaag gagatttttt gagtagacag tatattttta gaaaactatg atatgtactg
     5701 accccaaaag ttagattttt tctgtctaac ttttggggtg cagttcaata agggtgtata
     5761 aatttataaa ggaggctttc taatggaatg gtataaaaaa atcggacttc ttgcaactac
     5821 aggtttagct ttgtttgggc tcggcgcttg ctccaactat ggtaaatctg cggatggcac
     5881 agtgaccatc gagtatttca accagaaaaa agaaatgacc aaaaccttgg aagaaatcac
     5941 tcgtgatttt gagaaggaaa accctaagat caaggtcaaa gtcgtcaatg taccaaatgc
     6001 tggtgaagta ttgaagacac gcgttctcgc aggagatgtg cctgatgtgg tcaatattta
     6061 cccacagtcc atcgaactgc aagaatgggc aaaagcaggt gtttttgaag atttgagcaa
     6121 caaagactac ctgaaacgcg tgaaaaatgg ctacgctgaa aaatatgctg taaacgaaaa
     6181 agtttacaac gttcctttta cagctaatgc ttatggaatt tactacaaca aagataaatt
     6241 cgaagaactg ggcttgaagg ttcctgaaac ctgggatgaa tttgaacagt tagtcaaaga
     6301 tatcgttgct aaaggacaaa caccatttgg aattgcaggt gcagatgctt ggacactcaa
     6361 tggttacaat caattagcct ttgcgacagc aacaggtgga ggaaaagaag caaatcaata
     6421 ccttcgttat tctcaaccaa atgccattaa attgtcggat ccgattatga aagatgatat
     6481 caaggtcatg gacatccttc gcatcaatgg ctctaagcaa aagaactggg aaggtgctgg
     6541 ctataccgat gttatcggag ccttcgcacg tggggatgtc ctcatgacac caaatgggtc
     6601 ttgggcgatc acagcgatta atgaacaaaa accgaacttt aagattggga ccttcatgat
     6661 tccaggaaaa gaaaaaggac aaagcttaac cgttggtgtg ggagacttgg catggtctat
     6721 ctcagccacc accaaacatc caaaagaagc caatgccttt gtggaatata tgacccgtcc
     6781 agaagtcata caaaaatact acgatgtgga cggatctcca acagcgatcg aaggggtcaa
     6841 acaagcagga gaagattcac cgcttgctgg tatgaccgaa tatgccttta cggatcgtca
     6901 cttggtctgg ttgcaacaat actggaccag tgaagcagac ttccatacct tgaccatgaa
     6961 ctatgtcttg accggtgata aacaaggcat ggtcaatgat ttgaatgcct tctttaaccc
     7021 gatgaaagcg gatgtggatt aggagtagag agactatgaa aaaagtatta caaaaatatt
     7081 gggcatgggc ttttgtggtc atccccctct tgttacaagc aattttcttc tatgtgccga
     7141 tgtttcaagg agccttttac agttttacca actggacagg attgacttat aactacaaat
     7201 ttgttggctt aaacaacttt aagctcctct tcatggatcc aaaattcatg aatgcgattg
     7261 gctttaccgc aatcattacg attgccatgg tggttggtga gattgcactc gggatcttca
     7321 ttgcgcgtgt tttgaattct aaaatcaaag gccaaacctt cttccgtgct tggttcttct
     7381 tcccagctgt tttatctggt ttgacagtgg ctttgatctt caagcaagtc ttcaactacg
     7441 gtcttccagc gattgggaat gcccttcata ttgaatttct ccaaaccagt cttttaggga
     7501 ctaagtgggg agcaatcttt gcggctgtct ttgtccttct ttggcaaggg gtggctatgc
     7561 ccatcatcat cttcctagct ggtttgcaat ctattccaac tgagattaca gaggcagcaa
     7621 ggattgatgg tgcgactagc aagcaagttt tctggaacat tgaattgcct tacttgctac
     7681 caagtgtctc tatagtcttt atcctagccc taaaaggtgg gctgactgcc tttgatcaag
     7741 tctttgccat gaccggtggt ggtccaaaca atgccacaac ctcacttggg ctcttggttt
     7801 ataactatgc ctttaaaaac aaccaattcg gttatgccaa tgccattgcc gtaatcttgt
     7861 tcttcttaat tgtagtgatt tcgatcatcc aattgagagt atctaagaaa tttgaaattt
     7921 aagaggagaa gcatgatgaa acaagatgga agaaaagccc tgattggcaa atacattcta
     7981 ttgattctag gatcggttct gattttagtt ccgctccttg ctaccctctt tagttccttc
     8041 aaacccacta aggatattgt agataatttc tttggctttc caaccaactt cacatgggac
     8101 aactttagcc gtctcttagc tgatgggatt ggaggctatt attggaactt tgtcgtcatc
     8161 actgtcttgt ctttacttgc agtaatgatc tttatcccta tggcagccta ctccatcgct
     8221 cgcaatatga gtaaaagaaa agcctttacc atcatgtata ccctcttaat cctcggaatc
     8281 ttcgtacctt tccaagtcat catgattccg attacggtta tgatgagtaa actcggtttg
     8341 gctaatacct ttggtttgat cttgctctac ttgacctatg cgattccaca gaccctcttt
     8401 ctctatgttg gctatatcaa aatctcgatt ccagaaagtc tggatgaagc agcagagatc
     8461 gatggggcta atcaatttac aacctatttc cgcatcatct tcccaatgat gaaaccgatg
     8521 catgcgacaa ccatgatcat caatgccctt tggttctgga atgacttcat gttgccactc
     8581 cttgtcttga accgggattc caaaatgtgg actctgcctt tgttccaata caactacgca
     8641 ggccaatatt tcaacgacta cggaccaagc tttgcctctt acgtggtcgg cattatcagt
     8701 atcaccattg tctatctctt cttccaacgc catatcattt caggaatgag caacggggca
     8761 gtgaagtaat actcaatgaa aatcaaagag caaactagga agctagccgc aggctgtact
     8821 tgagtacggt aaggtgaagc tgacgtggtt tgaatttgat tttcgaatag tataagaaga
     8881 gctcatggaa tctatagtct ttgacacagc ggaagagttc agcttgttga taattctatg
     8941 tagaaaacaa gaaagtagta ctttgtttat cacagtctat attagtacga gtttgatgat
     9001 aaatagagat ttaaaatgaa tttgatttac gaagccaagt catatgagac ttggcttcaa
     9061 ttagaaaaag gagagtaatg atgccaattc aaaataaaac catgttgatt acctattctg
     9121 atagccttgg aaataatctt aaagacttat atgataattt ggaagagcat tttggagatg
     9181 ctattggagg agttcacctt ttaccatttt tcccatcaac aggtgatcgt ggatttgcgc
     9241 cagttgacta cgtcgaagtg gactcagctt ttggtgattg ggaggatgtt aagcgtttag
     9301 gtgagaaata ttatcttatg tttgatttta tgattaatca tatttctcgt caatccaagt
     9361 attataagga ctatcaagaa aaacatgaag ccagtgaatt taaagatctc tttttaaact
     9421 gggataagtt ttggccagaa aaccgtccga tacagtctga tgtagattta atttacaagc
     9481 gtaaggatcg tgcaccaaag caagagattg tgtttgaaga tggttcagtg gaacatttgt
     9541 ggaatacctt tggtgaggag cagattgatc ttgatgtgac caaagaagta actatggaat
     9601 ttatccgtaa gaccattcag cacttggcaa gtaatgggtg tgatttgatt cgtctagatg
     9661 cctttgctta tgcagtgaag aaattggata ctaatgattt ctttgtggaa ccagatattt
     9721 gggatttatt ggacaaagtt cgagatatcg ctgctgagta tgggacagag cttttacctg
     9781 agattcatga acactattcg attcagttta aaatagcaga ccatgattac tatgtttatt
     9841 attttgctct tccaatggtg acactttata ctctttacag ttccagaaca gagcgtttgg
     9901 ctaagtggtt aaagatgagc ccgatgaagc aatttacgac gctagatacc catgatggga
     9961 ttggagtagt agatgtcaag gatatcctga ccgatgagga gattgactat gcttcaaatg
    10021 aactctataa ggttggagcc aatgtcaaac gtaagtactc tagtgccgag tataacaact
    10081 tagatatcta ccaaatcaat tcaacctact attcagcgct tggagatgat gatgtcaagt
    10141 attttctcgc tcgtctaatt caagcttttg ccccaggtat tcctcaggtt tactatgtgg
    10201 gtctattagc aggcaagaat gacttgaaat tattagaaga aactaaagaa ggtcgaaata
    10261 ttaatcgtca ttactatagc aacgaggaaa tagcaaaaga agtgcaacga cctgttgtga
    10321 aggcccttct caatctattt tctttccgta accgttcaga agcctttgat ctagaaggga
    10381 ctactgagat agagacacca acagctcaca gcattgtaat caaacgtcaa aataaagata
    10441 agtccgtaac agcagtagca gaaattgatt tgcaaaatca gacttatcgg gtaattgaga
    10501 atggagttga agtaacattt tgaagccttg atatggtgga taaaataggg tttttatttt
    10561 ggaaaacgtt tcctttgttt tcaaattgct aaaaaagtgg tacaatatag gatagcttac
    10621 tattatctga atcagctgat ttggagagaa aggattcatt ttgaaatcaa taggctttat
    10681 tgaaaagctg aaggggttgt ctagtaaaga gctgatttta ttgggaatta tcctgagtat
    10741 ctttttaccc ttttatcttt ttgtagttgt actctgttta tatattatca gtttgatttt
    10801 tacaggagac atgaaaagta ttcttcagaa aatgggggag catccgatgc tgcttctttt
    10861 tcttagctat agtactgtta tatccattct tgcacaaaat tggatgggtc ttgtggcttc
    10921 agtaggaatg tttctattta ctattttctt tttgcactat cagtcgattt tatcccataa
    10981 attctttcga ttgattttgc agttcgtctt gtttggtagt gtcttgtcag ctgcttttgc
    11041 cagtttagaa catttccaaa ttgtgaagaa atttaactat gcttttcttt cacccaatat
    11101 gcaggtgtgg catcagaacc gggcagaagt gaccttcttt aatcctaatt attatggaat
    11161 tatttgttgt ttctgtatta tgattgcttt ctatctgttt acaacgacca agttgaattg
    11221 gttgaaagta ttctgtgtga ttgcaggctt tgttaatctc tttggtttga actttactca
    11281 aaatcgaact gcctttcctg ctattatcgc tggagcaatt atctatctct ttacgactat
    11341 taaaaactgg aaggcctttt ggcttagtat tggggtcttc gcgattggtt tgagtttcct
    11401 cttttctagt gatttgggag ttcgaatggg tactttagac tcttctatgg aagaacgcat
    11461 ttctatctgg gatgctggga tggccttgtt taagcaaaat cctttttggg gtgaagggcc
    11521 attgacctat atgcactctt atcctcggat acatgctcct tatcatgaac atgcccacag
    11581 tctttatatt gatacgattc tgagttacgg aattgtgggt accattttat tagttttgtc
    11641 ttctgttgct cctgttcgct tgatgatgga tatgagtcag gagtcgggga aacgtccgat
    11701 tatcggcctt tatctatctt tccttacagt ggttgctgtg cacggaattt ttgacttggc
    11761 tctcttctgg attcagtcag gctttatttt cttgctagtt atgtgcagta ttccattgga
    11821 gcatcgaatg ttggtatcgg acatgacgga ttaagtttta taagtttgaa atcttctacc
    11881 ttgggtagga gattttttta tacgatgggc atgtcgtgca tctgaggtgt aagtcctctg
    11941 tagggatcca ctaccggtga atacaatagc gctccccaag cctgactatc gtgaggtagc
    12001 ggattaaaat ggtctgggga tagatcgttt taagtctgac gcaggaaata agaattgtca
    12061 gagaaagcaa tagtgaaatc gtaggctatg aatagaaacg tgatagcgag gagcatatag
    12121 tggataaggt agcgagaaat tttaaagtat aaaagatagt cttaatctat atatgtaaat
    12181 cacaaaagta attgaattcg gaataaggtt ggtgtgaaaa gacatagtaa actgaatctt
    12241 gaatactaga atgatacttc tacaatattg ttataattta atttgaattc caaaatcaca
    12301 ttgttccgtc cgtatttcat tttactgtat gtcttcctaa actccaaagt ttctgcgtca
    12361 aatttcctat tttcactgta gtctttgaca cttcgaacga caatttgaat catatattca
    12421 aaaaaaaatg aaaaatttgt gccaacaagc ctaaatattc tgaaaatttg tttgattttt
    12481 aatggataat gtgatataat ggttgtcaat ctataattca cataaacaga ggagtagaaa
    12541 atgaaaaaaa atcgtgtatt tgctacagca ggtcttgttt tattagcagc aggtgtactt
    12601 gcagcatgca gttcttcaaa atcatctgat tcatcagccc ctaaagctta tggctatgtt
    12661 tatacagcag acccagaaac cttggactac ctgatttcaa gtaaaaatag tacaacagta
    12721 gtgacttcaa atgggattga tggtttattc actaacgata attacggtaa tcttgctcct
    12781 gcagttgcag aggattggga agtctctaag gatggtttga cctacactta taagattcgt
    12841 aaaggggtta aatggtttac ctctgatgga gaagaatatg cagaggtgac ggctaaagat
    12901 ttcgtgaacg gtttaaaaca cgcagcagat aaaaaatcag aagctatgta tttagctgaa
    12961 aattcggtta aaggcttggc agattatcta tcaggaactt caacagattt ttcaacagtt
    13021 ggtgtcaagg cggttgatga ttatacgtta caatacactt tgaaccagcc tgaaccgttc
    13081 tggaactcta agttgaccta ttctattttc tggcctctga atgaagaatt cgaaacatca
    13141 aaaggaagcg attttgctaa accaacagat ccgacatcct tgctttataa tggtccattc
    13201 ttgttgaaag ggttgactgc aaaatcttct gtagagtttg taaaaaatga gcaatattgg
    13261 gataaagaaa atgtccacct agatactatc aatctagctt actatgatgg atcagatcag
    13321 gagtcgctag agcgtaactt cactagtgga gcttatagtt atgcccgtct ttaccctacc
    13381 agctccaact attctaaggt tgcagaagaa tacaaggaca atatctatta cacacaatca
    13441 ggctctggga ttgctggtct gggtgtgaat attgatcgcc aaagttacaa ctatacttct
    13501 aaaactacag attcagagaa agtagctact aagaaggcat tgcttaacaa agatttccgt
    13561 caagccttga attttgctct tgatcgctca gcttactcag ctcaaatcaa tggtaaagat
    13621 ggagcagctt tagcagttcg taatttattt gtaaaaccag actttgtttc agctggtgag
    13681 aagacctttg gtgatttagt cgctgctcaa cttcctgctt atggtgatga gtggaaaggt
    13741 gtgaatttag ctgatgggca ggatggttta ttcaatgctg acaaggccaa ggcagagttt
    13801 gcgaaagcta agaaagcttt agaagcagac ggcgttcagt ttcctattca tctggacgtt
    13861 ccagtagacc aagcatcaaa aaactacata tctcgtattc agtcctttaa acaatctgta
    13921 gaaacagttc ttggtgttga aaatgtcgtt gttgatattc aacaaatgac aagtgatgaa
    13981 ttccttaata ttacttacta tgctgccaat gcttcatctg aggattggga tgtatcagga
    14041 ggagtttcat ggggtccaga ctatcaagac ccatctactt acctggatat tttaaaaaca
    14101 actagcagtg aaactacaaa aacatattta ggatttgata atccaaatag cccttcagta
    14161 gttcaagttg gtttgaaaga atacgataaa ttagttgatg aagctgccag agagacaagc
    14221 gacttgaatg tccgttatga aaaatatgca gcggctcaag catggttgac agatagttca
    14281 ctctttattc ctgctatggc ttcttctggt gcagcaccag tgctttcacg aattgttcca
    14341 tttactggag cttctgcgca aacaggctct aaggggtcag atgtttactt caaatatttg
    14401 aaattacaag ataaagtggt gactaaggaa gagtatgaaa aagctcgtga aaaatggttg
    14461 aaagaaaaag ctgaatcaaa tgagaaagct caaaaagaat tggcaagtca tgtgaagtaa
    14521 ataatttcta atagaacttt ctctccatga ggagaaggtt cttttgggat ttttaaagga
    14581 aataatatga aaaaatatat ttttatgcgt gttttgcggt cattggtttc gattttctta
    14641 gtaacgactt tgacctacac gattatctat accttggttc ctcgaaaatt gattttcaag
    14701 caggatccta actataataa aattgcgaca acggctgata aacgtgataa ctatgaaaat
    14761 actgtgtttg agcgtatggg ctacattgag tattacgata ctaaagagtt gcaagaaaag
    14821 gcaagtagca tggattcttc tgtaacagta gaagcaaatg cgaccaataa agctatttat
    14881 gaaaagtaca tcaatcaatt aggtcatggt tggactttgg gagaatttac tgaaagtggt
    14941 caattctatg ctactcgtga aattccaatt tttgaacgtg tttttcactt ctatgctaac
    15001 ttgattgaca ttgaccatac aaataaaatc caagaccctg aaaatccaga cttgaaacgc
    15061 taccttcgtt ttgaaaatga tccagctatc ggatggtcat tggtcggttc aggaactaaa
    15121 cataaatatc tcttgtactt taacagtcag ttcccatttg ttcatcaaaa ctttgtgaac
    15181 ttgaatttag gtgactctta cccaacctat gctaatacac cagttcttca ggttattact
    15241 caaggtcaag gacaaaccaa aactgcccaa gttcagttcc caacaggtaa gaaaacgtct
    15301 tctgtaaata tttactcaag aacctacaag tcacctagtc aggctgactc tcgtgaagta
    15361 gctagctatg ggaaagatga tccttataca gcgactgaaa gtaattacca atatccatct
    15421 atgattgtca gctctgctat tactggtttg attggtttgg ttcttgccta tgctcttgcc
    15481 gtgccacttg gttcagccat ggctcgtttc aagaacactt ggattgatag cctctcaaca
    15541 gggactttga ccttcttgct tgctcttcca acgattgcct tggtttacat cgttcgattg
    15601 attggatcat ctattgccct tccagattca ttccctatct tgggagctgg agattggcgt
    15661 tcttacgttt taccagcagt catccttggt ttgttgggtg ctcctgttac agccatttgg
    15721 attcgtcgtt acatgattga cttgcaatct caagactttg ttcgtttcgc tcgtgcaaaa
    15781 ggtttgtctg aaaaagaaat ttcaaacaaa cacatcttta aaaatgccat ggttccgctg
    15841 gtttcaggaa ttcctggtgc cattattggg gttatcggtg gtgcaaccct tactgaaaca
    15901 gtcttcgcct tcccaggtat gggtaaaatg ttgattgact ctgtaaaagc atctaataac
    15961 tctatggtcg ttggtcttgt cttcatcttt acatgtattt ctatcttctc acgtcttttg
    16021 ggagatattt ggatgactat tattgaccca cgtattaaat tgactgagaa aggaggcaaa
    16081 taatgtctac aatcgataaa gaaaaatttc agtttgtaaa acgtgacgat tttgcctctg
    16141 aaactattga tgcgccagca tattcttact ggaaatcagt gtttaaacaa tttatgaaga
    16201 aaaaatcaac tgtagtcatg ttgggaatct tggtagccat cattttgata agtttcatct
    16261 acccaatgtt ttctaagttt gatttcaatg atgtcagcaa ggtaaacgac tttagtgttc
    16321 gttatatcaa gccaaatgcg gagcattggt tcggtactga cagtaacggt aaatcgctct
    16381 ttgacggtgt ctggttcgga gctcgtaact ccatcctcat ttctgtgatt gcgacagtga
    16441 ttaacttggt tatcggtgtt tttgtcggtg gtatttgggg tatttcaaaa tcagttgacc
    16501 gtgtcatgat ggaagtttac aacgtcatct caaacatccc acctcttttg attgttattg
    16561 tcttgactta ctcaatcgga gctggattct ggaatctgat ttttgccatg agcgtaacaa
    16621 catggattgg tattgccttc atgatccgtg tgcaaatctt gcgctatcgt gacttggaat
    16681 acaacttggc gtcacgtact ttgggaacac caaccttgaa gattgttgcc aaaaatatca
    16741 tgcctcaatt ggtatctgtt attgtgacaa ccatgactca aatgcttcca agctttatct
    16801 catacgaagc cttcttgtct ttcttcggtc ttggattacc gattacagtg cccagtttgg
    16861 gtcgtttgat ttcggattat tcacaaaacg taacaaccaa tgcttacttg ttctggattc
    16921 cattgacaac ccttgtcttg gtatccttgt cccttttcgt agttggtcaa aacttagcgg
    16981 atgctagtga tccacgtaca catagatagg agtagaaatg acaaaagaaa aaaatgtaat
    17041 tttgactgct cgcgatattg tcgtggaatt tgacgttcgt gacaaagtat tgacagccat
    17101 tcgcggcgtt tcccttgaac tagtcgaagg agaagtatta gccttggtag gtgagtcagg
    17161 atcaggtaaa tctgttttga caaagacctt cacaggtatg ctcgaagaaa atggtcgcat
    17221 tgctcaaggt agtattgact accgtggtca ggacttgaca gctttatctt ctcacaagga
    17281 ttgggaacaa attcgtggtg ctaagattgc gactatcttc caggacccaa tgactagttt
    17341 ggaccccatt aaaacaattg gtagtcagat tacagaagtt attgtaaaac accaaggaaa
    17401 aacagctaaa gaagcgaaag aattggccat tgactacatg aataaggttg gcattccaga
    17461 tgcagataga cgttttaatg aatacccatt ccaatattct ggaggaatgc gtcaacgtat
    17521 cgttattgcg attgcccttg cctgccgacc tgatgtcttg atctgtgatg agccaacaac
    17581 tgccttggat gtaactattc aagctcagat tattgatttg ctaaaatctt tacaaaacga
    17641 gtatcatttc acaacaatct ttattaccca cgaccttggt gtggtggcaa gtattgcgga
    17701 taaggtagcg gttatgtatg caggagaaat cgttgagtat ggaacggttg aggaagtctt
    17761 ctatgaccct cgccatccat atacatggag tctcttgtct agcttgcctc agcttgctga
    17821 tgataaaggg gatctttact caatcccagg aacacctccg tcactttata ctgacctgaa
    17881 aggggatgct tttgccttgc gttctgacta cgcaatgcag attgacttcg aacaaaaagc
    17941 tcctcaattc tcagtatcag agacacattg ggctaaaact tggcttcttc atgaggatgc
    18001 tccaaaagta gaaaaaccag ctgtgattgc aaatctccat gataagatct gtgaaaaaat
    18061 gggatttgcc catctggctg actaggagga aggaaatgtc tgaaaaatta gtagaaatca
    18121 aagatttaga aatttccttc ggtgaaggaa gtaagaagtt tgtcgcggtt aaaaatgcta
    18181 acttctttat caacaaggga gaaactttct cgcttgtagg tgagtccggt agtgggaaaa
    18241 caactattgg tcgtgctatc atcggtctaa atgatacaag taatggagat atcatttttg
    18301 atggtcaaaa gattaatggt aagaaatcgc gtgaacaagc tgcggaattg attcgtcgaa
    18361 tccagatgat tttccaagac cctgccgcaa gtttgaatga acgtgcgact gttgattata
    18421 ttatttctga aggtctttac aatcaccgtt tatttaagga tgaagaagaa cgtaaagaga
    18481 aagttcaaaa tattatccgt gaagtaggtc ttcttgctga gcacttgact cgttaccctc
    18541 atgaattctc aggcggtcaa cgtcaacgta tcggtattgc ccgtgccttg gtcatgcaac
    18601 cagactttgt tattgcagat gagccaattt cagccttgga cgtttctgta cgtgcccaag
    18661 tcttgaactt gctcaaaaaa ttccaaaaag agctcggcct gacctatctc ttcatcgccc
    18721 atgacttgtc ggttgttcgc tttatttcag atcgtatcgc agttatttac aagggtgtta
    18781 ttgtagaggt tgcagaaaca gaagaattgt ttaacaatcc aattcaccca tatactcaag
    18841 ccttgctttc agcggtacca atcccagatc caatcttgga acgtaagaag gtcttgaagg
    18901 tttacgatcc aagtcaacac gactatgaga ctgataagcc atctatggta gaaatccgtc
    18961 caggtcacta tgtttgggcg aaccaagccg aattggcacg ttatcaaaaa ggactaaact
    19021 agtaatggtt ttataatttc catgtcaatt tttatggaaa ttaggctctt tgtcaactgt
    19081 agtgggttga agtcagctaa gctcgagaaa ggacaaattt cgtcctttct tttttgatgt
    19141 tcaaagcgat aaaaatccgt tttctgaagt tttcaaagtt tcgaaaacca aaggcattgc
    19201 gcttgataag tttgatgaga ttattggtcg cttccagttt ggcattagaa tagtgtagtt
    19261 gaagggcgtt gacaatcttt tctttatctt tgaggaaggt tttaaagaca gtctgaaaaa
    19321 gaggatgaac ctgctt
//
LOCUS       DAVKDZ010000036        18753 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000036 DAVKDZ010000000
VERSION     DAVKDZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18753
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.037"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(205..1014)
                     /locus_tag="VWZ30_001249"
     CDS             complement(205..1014)
                     /locus_tag="VWZ30_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9334984.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(1016..1819)
                     /locus_tag="VWZ30_001250"
     CDS             complement(1016..1819)
                     /locus_tag="VWZ30_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9334985.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSHENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(1829..3316)
                     /gene="tacF"
                     /locus_tag="VWZ30_001251"
     CDS             complement(1829..3316)
                     /gene="tacF"
                     /locus_tag="VWZ30_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEU9334986.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRAFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVLPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            3652..4359
                     /locus_tag="VWZ30_001252"
     CDS             3652..4359
                     /locus_tag="VWZ30_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU9334987.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            4364..5386
                     /locus_tag="VWZ30_001253"
     CDS             4364..5386
                     /locus_tag="VWZ30_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9334988.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            5403..6272
                     /gene="licA"
                     /locus_tag="VWZ30_001254"
     CDS             5403..6272
                     /gene="licA"
                     /locus_tag="VWZ30_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEU9334989.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAIRLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGADFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            6262..7140
                     /locus_tag="VWZ30_001255"
     CDS             6262..7140
                     /locus_tag="VWZ30_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU9334990.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKIRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFVAVANGQL
                     LGLMIVFVAFDMISYLAYYIVINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            7152..7841
                     /locus_tag="VWZ30_001256"
     CDS             7152..7841
                     /locus_tag="VWZ30_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP--phosphocholine cytidylyltransferase"
                     /protein_id="HEU9334991.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DASTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            7938..8786
                     /gene="dprA"
                     /locus_tag="VWZ30_001257"
     CDS             7938..8786
                     /gene="dprA"
                     /locus_tag="VWZ30_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU9334992.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIANIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            9670..10713
                     /locus_tag="VWZ30_001258"
     CDS             9670..10713
                     /locus_tag="VWZ30_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEU9334993.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            10880..12967
                     /gene="topA"
                     /locus_tag="VWZ30_001259"
     CDS             10880..12967
                     /gene="topA"
                     /locus_tag="VWZ30_001259"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU9334994.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFDS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            13188..13547
                     /locus_tag="VWZ30_001260"
     CDS             13188..13547
                     /locus_tag="VWZ30_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU9334995.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            13640..14272
                     /locus_tag="VWZ30_001261"
     CDS             13640..14272
                     /locus_tag="VWZ30_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU9334996.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            14316..14972
                     /locus_tag="VWZ30_001262"
     CDS             14316..14972
                     /locus_tag="VWZ30_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU9334997.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMRISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTYKK
                     "
     gene            14991..16085
                     /locus_tag="VWZ30_001263"
     CDS             14991..16085
                     /locus_tag="VWZ30_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU9334998.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTPEEYGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAGNAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALVDKKQEITDADI
                     RALVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIF
                     NAIDKFFNQSVRLVSYTIDAVTDGIDTQDRVLVTVENRDTETIFNAAGLDFDVLKASA
                     IVYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            16097..17134
                     /gene="leuB"
                     /locus_tag="VWZ30_001264"
     CDS             16097..17134
                     /gene="leuB"
                     /locus_tag="VWZ30_001264"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU9334999.1"
                     /translation="MAKKIVALAGDGIGPEIMEVGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAWPPLPDETLKASREADAILLAAIGSPQYDGAVVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKHLSPLKLERIAGVDFVVVRELTGGIYFGDHILEERNARDINDYSYEE
                     VERIIRKAFEIARNCRKILTSIDKQNVLATSKLWRKVAEEVAQDFSDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSILSGTLGVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPISMILSVVMMLRDSFGRYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMMEAIIARL"
     gene            17131..17400
                     /locus_tag="VWZ30_001265"
     CDS             17131..17400
                     /locus_tag="VWZ30_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEU9335000.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            17404..17763
                     /gene="leuD"
                     /locus_tag="VWZ30_001266"
     CDS             17404..17763
                     /gene="leuD"
                     /locus_tag="VWZ30_001266"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU9335001.1"
                     /translation="MAGSSREHAAWALADYGFKVVIAGSFGDIHYNNELNNDLLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            17925..18495
                     /locus_tag="VWZ30_001267"
                     /pseudo
     CDS             17925..18495
                     /locus_tag="VWZ30_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019771122.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            18589..18654
                     /locus_tag="VWZ30_001268"
                     /pseudo
     CDS             18589..18654
                     /locus_tag="VWZ30_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
BASE COUNT         5873 a         3207 c         3969 g         5704 t
ORIGIN      
        1 aatttgaatt ttctaattga ttcgttcgca tcttatttca atctactata gaaaaaagtc
       61 tttaaaatta taaaacgcat catatcaagg tttttcaaaa accctgatat gatgcgtttt
      121 attgtgggaa tatgtatttc attttctact aaaattatgt ttttgaataa cctctatctt
      181 agtagtttgt ataatccccc tcaatcagtt tttgcgataa gctttaatgc tatgactata
      241 ccactcttgc atttcttttg atggtggtgt catataatcg ccatacatct gggttaaaaa
      301 ttggtcatat tttttgggaa caggcaacat acggccctca aactcagtta aaatcagttc
      361 tttaaaggta tcaactggga agatttcttt catcccttcc ttaccgatcc caactcctcc
      421 ttcatattga ggagtgttgg ttacagcatt tttgactagt tgatcaattt tcttgtaaaa
      481 gtagcgagga ttgacaaatc ggagagcgta ccaactacat aatctaagaa aatcttttag
      541 tttgctatca ccgtgaactg ctcgtgattt tttgatataa gctagttgac gaagggccac
      601 atacttatag ctcttgtcga caatgctcaa gtctgtaaat cgatcaattg ggaagacatc
      661 gatgaaaagg ctggtatcat gacgcttgta cttaacatgg tcttctataa cagtagaagt
      721 gtccaaaatc gatgcgaaat tatggaagta ccaagaagat gtatcgtagg aaagaacctt
      781 gtagcgaggg tgattttctt cttcaataat cttcagtaaa cgctcataat cctcacgata
      841 aagggaaata tcaatatcat catcccaagg aatcatacct ttgtggcgga tggctccaag
      901 catggttcca taactgagaa aataaggaat atcatgtttc ttacaagtct catcaatata
      961 gtccagcagg gctagttgaa tttctttaat ttcttttttt tctaaatatt gcatcctaat
     1021 cctccaattt ataagcgtga aattcatgac tgtagaagcg tttttcttct ggtggtaggg
     1081 tcatataatc tccataaaat tgtgtcaaaa tagtatcaaa tttttcaggt gcaggaaggc
     1141 ttaaattctc aaagggtaaa tcgattgttt tatcaaaggt accacttggg aagacttcct
     1201 tttccttaaa ttttgaaggg ataaaagcca tatactgccc attttcatga ctatattttt
     1261 gaatttcttt ctcgatttta tttgcaaaat aacgaggaga aaccggtcga aggagcaacc
     1321 aaaaggctgt tcgtatccaa tcttttaaaa ggctatcctt atagacaata tttttatgtt
     1381 tactgaaaga cagcagtttg aagctttcca gtttataaca agtatcaatg accttaggat
     1441 catcaaagcg atctataggg aaaatatcga taaagatacc agactcatag gtttttgtat
     1501 ttcgagtatc aataatttta gtcgtactgt cggttatctt gataaagttg ttaaagtagt
     1561 tcttatcagt ttctaaggat aggagcttat acttgctttt ttccttttga aaaatgttaa
     1621 taaatcgttg gtagtcttct ctaggcatgg ataaatcaat atcgtcgtcc caagggataa
     1681 agccctcatg tcgaaccgcc ccaatcagag taccgtagtt aataatatag ttgatattgt
     1741 gctttttaca gagagtatca atataatcca aaatttctaa ttcaatttgt ttggcatctt
     1801 caacggttag ttgtttcatt ttaaactcct atgatttttt aaatttattt tttaaggcta
     1861 ggacatggtt taaaaattca tagaaaatgc tatcttttgt gaagacaagt agactaatat
     1921 aagagatagc tgatagcaag acaatcaaac cagtattaat taaaaatggc aaattaatga
     1981 ccatatccac aggatacacg aaattaatca ggaaataaat tgctacaaag gaaagtgaaa
     2041 agagagagta tcgaacagta tagctaaaga tatgtcccaa gtggatgagt tgttttctat
     2101 ggatgaaaat aatatagaaa acaagtagag aagtctctga taacatagtt gtcagtaagt
     2161 agtattcagg aagcacgata tggttgaaaa agaggagact attcaagccc aaattgagta
     2221 gcccagcaaa gactgtatag actgtgatac gtttttcata gccattggta aagagaattt
     2281 gggaaccaag aatggtatct aaggccagga taatcgtacg aaaagcgaag agagaggtca
     2341 agatgccgcc tccgatatat ttttcactac cgtaaagtag gatggcattt ggtcctaaaa
     2401 ccatgagtcc aaaactcagt ggaatgataa agaagttaaa gattcgacta cctctattaa
     2461 ccagagaaac ataggcttct ttgtctcctt tccccagata gtaactgaga cgaggcacac
     2521 tcactccaat tgcacctgtt acaaccccag ctataacggt cacaattcgc tgagctatgg
     2581 tatagtaact aacgttgaca tcaatccctg ttttaacgag gaagaggcga tctaaaaaag
     2641 tgaagagcat attggcattg gcaaagacta acatggctgt cagagggaga aagagtggtt
     2701 taaaatcact taggtgaatc ttaacaagtt tgatgtctct tttaatccaa aaataactaa
     2761 tcaggtagtt aatcagcgtc gataaactca tcacaagtgt atagacaaca atatcgtgtt
     2821 catttttaac aaataagaaa atagagacca gcatcaggat acggatgaag gcagttttgt
     2881 aaaagagaaa actgtaattt tccagagctt cattgaccca ttcgattgaa aaaatctggg
     2941 caatgagttg aatccccata acaaggtaga cctttttgac gattggatta tcagtaaaga
     3001 agagaggata ggctaggata tagacagcag tggtcaaaat cgtacaagcg atgcacaaat
     3061 aaaaaagact agaaaaggct ctgttaagat cttttttgtt atccttgaca ttactgatag
     3121 cccttaaacc gtagttatag acaccataag ttgcaaaggg caagaaaaat gacaaaatag
     3181 tgtcgactga gttgaagtaa ccatagtcag ttcggtccaa gacacgcgcg acataggttc
     3241 cagttaggat gggaaaaata atattcaaga cacgaattcc catgtaagat agagcattta
     3301 attttatact tttcattcaa tttacctcgt ttttcattat atcataaagt tagctaataa
     3361 gaaatgaagg gcagtaagtc aagtaatcac tttgaagttt caaatcttaa attttaagtt
     3421 ttctttaagg aaagtatatt attctgaagg actctaaaat ttcgcaacca tttattagta
     3481 attgctacag aattcctagt cattactaga aatggactag tttctttgaa taatagaact
     3541 gcataattct cctattctag aaggggagga ccagtatttc ttttatgata ggactagatt
     3601 gtggtataat agagagaata agttttttta gtaagacaaa ggagaaaata gatgatttat
     3661 gcaggaattc ttgctggtgg aactggcaca cgcatgggaa tcagtaactt gccaaaacaa
     3721 tttttagagc taggtgatcg acctattttg attcatacaa ttgaaaaatt tgtcttggag
     3781 ccaagtattg aaaaaattgt agttggtgtt catggagact gggtttctca tgcagaagat
     3841 cttgtagata aatatcttcc tctttataag gaacgtatca tcattacaaa gggtggtgct
     3901 gaccgcaata caagtattaa gaaaattatt gaagcgattg atgcttatcg tccgcttact
     3961 ccagaggata tcgttgttac ccacgattct gttcgtccat ttattacact tcgcatgatt
     4021 caggacaata tccaacttgc ccaaaatcat gacgcagtgg acacagtggt agaagcggtt
     4081 gatactatcg ttgaaagtac caatggtcaa tttattacag atattccaaa tcgtgctcac
     4141 ctttatcaag gacaaacacc tcaaacattc cgttgcaagg acttcatgga cctttatgga
     4201 tctctttctg atgaagagaa ggaaatcttg acagatgcat gtaaaatctt tgtgatcaaa
     4261 ggaaaagatg tggccttggc caaaggcgaa tactcaaatc tgaagattac aaccgtaaca
     4321 gatttgaaga ttgcaaaaag tatgattgag aaagactagt aaaatgatta atcaaattta
     4381 tcaactaact aagcctaagt ttatcaatgt caaatatcag gaagaggcta ttgaccaaga
     4441 gaatcatatc cttatccgtc ccaactacat ggctgtctgt catgcggatc agcgttacta
     4501 tcagggaaaa cgtgatccca agattttgaa taaaaagctt ccaatggcga tgattcacga
     4561 gtcatgtgga accgtcattt ctgacccgac cggaacctac gaggttggtc aaaaagttgt
     4621 catgattccc aatcagtctc ctatgcagag tgatgaagaa ttctatgaaa actacatgac
     4681 agggacccat ttcttgtcta gtggatttga tggctttatg agagagtttg tttctctccc
     4741 taaagatcgt gtggtggctt atgatgctat tgaagatacg gttgcagcca ttacagagtt
     4801 tgtcagtgtg ggcatgcacg ctatgaatcg tctattgact cttgctcata gcaagcggga
     4861 gcggatcgcc gttattggag atggaagttt agcttttgtg gttgccaata ttatcaacta
     4921 tactttgcca gaatcagaga ttgtggttat tggtcgtcat tgggaaaagt tggaactctt
     4981 ctcatttgcc aaagaatgct atattacgga taatattcct gaagatttgg cctttgacca
     5041 tgcttttgaa tgttgtggtg gtgatggtac tggaccagct attaatgact tgattcgcta
     5101 cattcgtcct cagggaacga ttctcatgat gggagttagc gaatataaag tcaatctcaa
     5161 tactcgcgat gccttagaaa agggcttgat tttggttggg tcatctcgtt ctggtcgcat
     5221 tgattttgaa aatgctatcc aaatgatgga agtcaagaaa tttgccaatc gtcttaaaaa
     5281 tatcctttat ctagaagaac ctgtaagaga aattaaagat attcatcgtg tctttgcaac
     5341 cgatttaaac acagccttta aaacagtgtt taagtgggaa gtataagtac tggaggttaa
     5401 ttgtggagaa aatcattaaa gaaaaaattt cttccttact tagtcaagaa gaggaagtcc
     5461 tcagtgttga acaactgggt ggaatgacca atcaaaacta tttggccaaa acaacaaata
     5521 agcaatacat tgttaaattc tttggtaaag ggacagaaaa gcttatcaat cgacaagatg
     5581 aaaagtacaa tcttgaacta ctaaaggatt taggcttaga tgtaaaaaat tatctttttg
     5641 atattgaagc tggtatcaaa gtaaatgagt atatcgaatc tgcgattagg cttgattcaa
     5701 cgtcaatcaa gaccaagttc gacaaaattg ctccaatatt acaaactatt catacgtctg
     5761 ctaaggaatt aagaggagaa tttgctcctt ttgaagaaat caaaaaatac gaatccttga
     5821 ttgaagaaca aattccttat gccaactatg aatctgttag aaatgcagtc ttctccttag
     5881 agaaaagact ggctgactta ggtgttgaca gaaaatcttg tcatatcgat ttggtgcctg
     5941 aaaactttat cgaatcacct caaggacgac tttatttgat tgactgggaa tattcatcaa
     6001 tgaatgatcc aatgtgggat ttggctgccc tctttttaga gtctgaattc acttcccaag
     6061 aggaagaaac tttcttatct cactatgaga gtgaccaaac accggtttct catgaaaaga
     6121 ttgctattta taaaatttta caagatacta tttggagtct atggactgta tataaggaag
     6181 agcaaggtgc agattttggt gactatggtg tgaatcgtta ccaaagagct gttaaaggtt
     6241 tggcttctta tggaggttca gatgaaaagt aaaaacggag ttccttttgg ccttctctca
     6301 ggtattttct ggggcttggg tctaacagtt agtgcttata tcttttcgat ttttacagat
     6361 ttgtcaccct ttgtggtggc cgctgctcac gatttcttga gtatctttat cttactggct
     6421 tttcttttag taaaagaagg gaaaattcgt ctctcaattt tcttaaatat tcgcaatgtc
     6481 agtgttatca tcggagcctt gctagcaggt cctattggta tgcaggccaa tctttatgca
     6541 gttaagtata tcggaagttc tttagcttca tctgtatcgg ctatttaccc tgcgatttca
     6601 gttctattgg ctttcttctt tttgaagcac aagatttcga aaaatactgt atttgggatt
     6661 gtcttgatta ttggagggat tattgcccag acctataagg ttgaacaggt taattccttc
     6721 tacattggga ttctctgtgc tttggtttgt gctattgcat ggggaagtga gagtgttctt
     6781 agctcctttg ctatggaaag tgaactgagt gaaattgaag ccctcttaat ccgtcaagtg
     6841 acttcgttct tgtcctatct tgtgattgtg ctcttctctc atcagtcatt tgttgcagta
     6901 gccaatggac aattgctagg tctcatgatt gtctttgtag cctttgatat gatttcctac
     6961 ttggcttatt atatcgttat caatcgcttg caaccagcca aggctacagg cttgaacgtg
     7021 agctatgtag tatggacggt cttgtttgca gttgtttttt tgggtgcacc gctagatatg
     7081 ctgaccatta tgacgtcact tgtcgtcatt gctggagttt atattattat taaagaataa
     7141 aggagattcg tgtgaaagcc attatcttag cagcgggatt gggaactcgc ttgcgtccta
     7201 tgactgaaaa tacccctaaa gccttggttc aggttaatca aaaacctttg attgaatacc
     7261 aaattgagtt tctcaaagaa aaaggaatca atgacatcat catcatcgtt ggttatctta
     7321 aagaacaatt cgattattta aaagaaaaat acggtgttcg cctcgttttc aatgataaat
     7381 acgctgacta caataacttt tactctctct atcttgtaaa agaagaattg gccaacagct
     7441 atgttattga tgctgacaat tatctcttta aaaatatgtt ccgcaatgat ttgacacgtt
     7501 cgacttattt tagtgtttat cgtgaagatt gtaccaacga atggttcttg gtctatggag
     7561 atgactacaa ggttcaagac attattgttg atagcaaggc aggtcgcatc cttagtggtg
     7621 tatccttctg ggatgcttca actgcagaaa agattgtcag ctttatcgac aaggcttatg
     7681 caagtggtga atttgttgat ctctattggg acaatatggt taaggataat atcaaagagc
     7741 tagatgtcta tgttgaagaa ttagaaggca atagcattta tgagatcgat agtgtccaag
     7801 actatcgtaa attagaagaa attcttaaaa acgaaaatta aagattccaa catctgacaa
     7861 aatagtcgga tgtttttttg attttttacg aacttttacg aatagataga tgagtagaaa
     7921 aagaaatgga gttatttatg aaaattacaa actatgaaat ctataagtta aaaaaatcag
     7981 gtttgaccaa tcaacagatt ttgaaagtgc tagaatacgg tgaaaatgtt gatcaggagc
     8041 ttttgttggg tgatattgca aatatatcag gttgccgtaa tccagccgtt tttatggaac
     8101 gttattttca gatagacgat gcgcatttgt cgaaagagtt tcaaaaattt ccatctttct
     8161 ctattttaga tgactgttat ccttgggatt tgagtgaaat atatgatgcg cctgtacttt
     8221 tattttacaa gggaaatctt gacctcctga aattcccgaa ggtagcggtc gtgggcagtc
     8281 gtgcttgtag caaacaggga gctaaatcag ttgaaaaagt cattcaaggc ttggaaaatg
     8341 aactggttat tgtcagtggt ctggccaagg gcattgacac agcagctcat atggcagctc
     8401 ttcagaatgg cggaaaaacc attgcagtga ttggaacagg actggatgtg ttttatccta
     8461 aagccaataa acgcttgcaa gactacatcg gcaatgacca tctggttcta agtgaatatg
     8521 gacctggcga acaacctctg aaatttcatt ttcctgcccg taatcgcatc attgctggac
     8581 tttgtcgtgg tgtgattgta gcagaggcta agatgcgttc aggtagtctc attacctgtg
     8641 agcgagcaat ggaagaagga cgcgatgtct ttgctattcc tggtagcatt ttagatggac
     8701 tatcagacgg ttgccatcat ttgattcaag aaggagcaaa attggtcacc agtgggcaag
     8761 atgttcttgc ggaatttgaa ttttaaaaat gacctaagct agaattctaa gaaaaaatca
     8821 attttaagag aaaatgaacc caacatttcc ataataaaac gcatattagc aagtttttaa
     8881 cacttgataa tatgcgtttt ttctaagtgg attagtagag tagaggattt ttctcatata
     8941 atactcttcg aaaatctctt caaactacgt cagcttccat ctgcaacctc aaaacagtat
     9001 tttgagctga cttcgtcagt cttatctaca acctcaaagc agtgctttga gcaacctgtg
     9061 gctcgcttcc tagtttgcgc tttgattttc attgagtata agggaaagta tagtgaattg
     9121 aaataagatg tgaacaactc tatcaggaaa gtcaaattga tttatagaaa tattttagca
     9181 gccaaggtgc actgttatag attcaataca ctataattta gtgtaattga gaaaggagaa
     9241 atgattgtga ttgatgttgg ctaggttatg ttcaatgatt cctaccgtct caaatcttgt
     9301 cagtaaggaa aaataaattc ttcaaaagta gagattacaa ggcttgttta agaaagaatt
     9361 caaagacctt gacaaataaa aataaaatgg ttattataaa aaatggtctg aaatagatga
     9421 tgatactttt cgaaaatctt tttacgtcag ctcagctttg ccttgctgtg ttttgagcaa
     9481 gctacggtta gcttccgagt ttgattttca tttactagaa atgaaactga tgagagatat
     9541 cagtagacat ctgagttagg atattatgga aaatgataaa aagagctcgt gagattggca
     9601 tatcagacta ctaaagtatt gagtttgtta ggattttagc gactagttag ctgggaaagg
     9661 aagatatttg tgacaaataa taaactgtat tcgttgatag aatttagaaa taaaatatat
     9721 gaagaattag aactttccag aagtgattta gcgattttac tatgtgccat gcttatcgcc
     9781 tctatcggat taaatatgga ttcgactccc gtgattattg gagccatgtt aatctctcct
     9841 ttgatgacac ctattctggg agtggggctc tctctagcta tatttgattt taaattgtta
     9901 agaaaatctt ttaaaatatt agctattcaa attcttgcca gtctaatagc ttcaacactt
     9961 tatttttatc tttctcccat ttcgtatgct agttcggaga ttgttgctag aacctctccg
    10021 actatttggg atgttctcat tgcttttgta ggagggatag caggtattat tggtgctagg
    10081 aaaaaagaga ccaataatat tgttcctggt gttgctattg caaccgcctt gatgcctcct
    10141 ctttgtacag taggttatgc tattgcttct gctaatctaa aatttatcat aggctcctct
    10201 tacctattcc tcatcaattg tagctttatt gtcattgcga cttatatagg tgttaggttg
    10261 atgatggtta agaaacatta ttttaaagat aatgaagaag actctaaaat gcgtaggatt
    10321 ttgcttctag ttgctgtttt gctgatgatt ccgagtttca tctctgcaac gactttagtg
    10381 agagaaacgt tgaaaaaaga gtcccttaag aaatttatat cagagcagtt tcaggggtat
    10441 aatattttga aaaaaaccta ttctaaaaag actcataccc taaagctaac catttcagga
    10501 aattatttga cagaagaaga actcgatatg atttcaagta agagaggtga ctatggttta
    10561 agtgatgttt ctgttcaagt ttcacaattg tctgattcag aacaacttag taaggaagag
    10621 ctggtggagt atttcttcca gtatatcaag gataaggaag caaaagaaaa ggagaaagct
    10681 aataaatttg atacagagtc tgaggagcaa taatttcttg agaatagctg gtttctctcg
    10741 tgagtcttct atgtatatca aaggaagact gaggttttaa gtataaaact tttcttctat
    10801 tatagtagct aagtcttgac agggttcaaa aaatggttta cactttataa agtttattac
    10861 tttgaaaagg tgtgatactg tggctacggc aacaaaaaag aaaaaatcaa cagttaaaaa
    10921 aaatctagtc atcgtggagt cgcctgctaa ggccaagacg attgaaaaat atctaggcag
    10981 aaactacaag gttttagcca gtgtcgggca tatccgtgat ttgaagaaat ccagtatgtc
    11041 cgtcgatatt gaaaataatt atgaaccgca atatattaat atccgaggaa aaggccctct
    11101 tatcaatgac ttgaaaaaag aagctaaaaa agctaataaa gtttttctcg cgagtgaccc
    11161 ggaccgtgaa ggagaagcga tttcttggca tttggcccat attctcaact tggatgaaaa
    11221 tgatgccaac cgtgtggtct tcaatgaaat caccaaggat gcagtcaaaa atgcttttaa
    11281 agaacctcgt aagatcgata tggacttggt cgatgcccaa caagctcgtc ggatcttgga
    11341 tcgcttggta gggtattcga tttcgcctat tttgtggaag aaggtcaaga agggcttgtc
    11401 agcaggtcgc gttcagtcca ttgcccttaa actcatcatt gaccgtgaaa atgaaatcaa
    11461 tgccttccag ccagaagaat actggacagt tgatgctgtc tttaaaaagg gaaccaaaca
    11521 atttcatgct tccttctatg gagtagatgg taaaaagatg aaactgacca gcaatgacga
    11581 agtcaaggaa gtcttgtctc gtctgacgag taaagacttt tcagtagatc aggtggataa
    11641 gaaagagcgc aagcgcaatg ctcctttacc ctataccact tcatctatgc agatggatgc
    11701 tgccaataaa atcaatttcc gtactcgaaa aaccatgatg gttgcccaac agctctatga
    11761 aggaattaat atcggttctg gtgttcaagg tttgattacc tatatgcgta ccgattcgac
    11821 tcgtatcagt cctgtagcgc aaaatgaggc ggcaagcttc attacggatc gttttgatag
    11881 taagtattct aagcacggta gcaaggtcaa aaacgcatca ggtgctcagg atgcccatga
    11941 ggctattcgt ccgtcaagtg tctttaatac accagaaagc atcgctaagt atctggacaa
    12001 ggatcagctc aagctatata cccttatctg gaatcgtttt gtggctagcc agatgacagc
    12061 ggccgttttt gataccatgg ctgttaaatt gtctcaaaaa ggggttcaat ttgctgccaa
    12121 tggtagtcag gttaagtttg atggttatct tgccatttat aatgattctg acaagaataa
    12181 gatgttaccg gacatggttg ttggagatgt ggtcaaacag gtcaatagca aaccagagca
    12241 acatttcacc caaccgcctg cccgttattc tgaagcaaca ctgattaaaa ccttagagga
    12301 aaatggggtt ggacgtccat caacctacgc gccaaccatt gaaaccattc agaaacgtta
    12361 ttatgttcgc ctggcagcca aacgttttga accgacagag ttgggagaaa ttgtcaataa
    12421 gctcatcgtt gaatatttcc cagatatcgt aaacgtgacc ttcacagctg aaatggaagg
    12481 taaactggat gatgtcgaag ttggaaaaga gcagtggcga cgggtcattg atgcctttta
    12541 caaaccattc tctaaagaag ttgccaaggc tgaagaagaa atggaaaaaa tccagattaa
    12601 ggatgaacca gctggatttg actgtgaagt gtgtggcagt ccaatggtca ttaaacttgg
    12661 tcgttttggt aaattctacg cttgtagcaa tttcccagat tgccgtcata cccaagcaat
    12721 cgtgaaagag attggtgttg agtgtccaag ctgtcatcag ggacaaatta ttgagcgaaa
    12781 aaccaagcgt aatcgcctat tctatggttg caatcgctat ccagaatgtg aatttacctc
    12841 ttgggacaag cctgttggtc gtgactgtcc aaaatgtggc aacttcctca tggagaaaaa
    12901 agtccgtggt ggtggcaagc aggttgtttg tagcaaaggc gactacgagg aagaaaagat
    12961 taaataagag gaggggcccg aaagtaaatt tcaggctcct tttggttaga acttgacaaa
    13021 atacatcatt ttatgcgaaa ctagaagaag attattttat actcaatgag aatcaaagag
    13081 caaattagaa agctagtcgc aggttgctca aagcactact ttgaggttga agatggaagc
    13141 tgacctggtt tgaagagatt ttcgaagagt attaactagg agaagttatg cgtcttatct
    13201 atttgataat tggtttttta tcactggcct tggctattgt tggggttgtt ttacccttgt
    13261 tgccgacaac gcctttcctt ttgttgtcta ttgcttgttt ctccagaagt tccaagcgtt
    13321 tcgaagactg gctttatcat accaagctct atcaaacata tgtagctgat tttcgcgaga
    13381 ctaagtctat tacgcgtgaa cgaaagaaaa aaatcatcgt ctctatctac gtcttgatgg
    13441 gaatttctat ttattttgca cctcttttac cagtcaaaat cggtctgggt gctttgacca
    13501 tctttatcac ttattatctc ttcaaggtca ttccagacaa agaatagtta aaacagtagt
    13561 tatttgcctt gataaaattg aaatcatatt cataacaata tgatataata aatttgaagt
    13621 aatattcaag gagaatcaaa tgatttacga attttgtgct gaaaatgtga ctttacttga
    13681 aaaggcgatg caggctggag ctcgtcggat tgaactctgt gataatctag cagttggtgg
    13741 gacaacaccc agctatggag tgactaaggc agcggttgaa ctggcagcta actacgatac
    13801 aaccatcatg accatgattc ggccacgtgg tggtgacttt gtctataatg acctagaaat
    13861 tgctatcatg ctagaagaca ttcgtttgac tgctcaggct ggaagtcaag gggttgtatt
    13921 tggagcttta actgctgata aaaagttgga taagcctaat ctggaaaagt taattgctgc
    13981 atcaaaagga atggaaattg tctttcacat ggcctttgat gaactaagtg atgaagatca
    14041 accggaagct attgactggc tcagtcaagc cggtgtcact cgtatcctaa ctcgtgctgg
    14101 tgtgtctggc gactccttag aaaaacgttt tgttcactat cacagaattt tggagtacgc
    14161 taaaggtaaa attgaaattc taccaggtgg ggggattgac cttgaaaacc gtcaaacctt
    14221 tatcgaccag gtgggggtaa cacaattgca tggtactaag gttgtttttt aaaaaataga
    14281 aaggaactgc tagctttggg tagcagtttt cacttatgtt tgaaattttt aaatcctatc
    14341 aatttaatca agaaaaggct catgattatg gttttataga aaatagcgaa gtctggacat
    14401 atagttgcca gattttgcaa ggtgactttg tcatgactgt gtccatcact gctgataatg
    14461 tgaactttca agtctttgac caagagactg gtgacctcta tcctcacgtt catatggaaa
    14521 gcatgagggg aagttttgtc ggaaatgtcc gtgaggcttg tctggagatt ctttaccaga
    14581 ttcggaaggc ttgttttgat gtgcaagatt ttatctgtca tcagactaag cgtatcatga
    14641 ctcaagttca ggaaaagtat ggaaaccagt tggagtatct gtgggaaaaa tcgcctgata
    14701 cagctgtatt gcgccatgaa ggcaatcaaa agtggtatgc cgtcttgatg agaatctctt
    14761 ggaataagct ggaaaagggt agagaaggac aagtggaagc agtcaacctc aagcatgacc
    14821 aagtagctaa tttgctttca caaaagggga tttatccagc ctttcatatg agcaagcgct
    14881 actggattag tgtgtccctt gatgatactt tatcagatga agaagtactg gaattgatag
    14941 aaaaaagttg gaacttaacc tataaaaaat gaaatatttt aataattttc atgaactttc
    15001 aattagctaa atattcttta ctgaagaaat tttcagaaaa tataggattt accacaccag
    15061 aggaatatgg tgccatcttc aaatacctga ttgagaatgt caagacggat cgtcagatca
    15121 tctattcgcc tcactgtcat gatgacctcg gaatggcagt ggcaaatagc cttgctgctg
    15181 tcaagaatgg tgcaggacgt gttgaaggga ctatcaatgg tattagggag cgagctggaa
    15241 atgctgcttt ggaagaaatt gcagtggctc tcaatattcg ccaagattac taccaagtag
    15301 aaaccagtat tgtcctaaat gagaccatca atacgtcaga aatggtttct cgcttctctg
    15361 gtattccagt tcctaaaaac aaagccgtcg ttggtggcaa taccttctcc cacgaatctg
    15421 gtattcacca agatggagtc cttaaaaatc ctctcactta tgagatcatc acacctgaat
    15481 tggttggtgt taagattccg cttggaaaat tatctggtcg ccatgctttt gttgagaaac
    15541 tgagagaatt ggccctagat tttacagaag aggatatcaa accactcttt gctaagttca
    15601 aggcactggt cgataagaag caagaaatca cagatgcaga tattcgagct ttggtagctg
    15661 gaaccatggt tgaaaatcca gaaggcttcc actttgatga tttacaactt caaactcatg
    15721 cagataatga cattgaagcg ctcgttagcc tagccaatat ggatggtgag aaagtcgaat
    15781 ttaatgcgac agggcaaggt tccgttgaag caatctttaa cgctatcgat aagttcttta
    15841 accaatctgt tcgtttggtg tcctacacta tcgatgcggt aacagatgga atcgataccc
    15901 aggatcgggt tttggtcact gttgaaaaca gagatacaga aaccatcttt aatgcagcag
    15961 ggcttgattt tgatgtgttg aaggcttctg ctattgtcta tataaacgct aatacctttg
    16021 ttcaaaaaga gaatgcaggt gagatgggac gcagtgtttc ttaccacgat atgcctagtg
    16081 tgtaaaggag aaggctatgg caaagaaaat agtagctcta gcaggagacg gaattggccc
    16141 agaaatcatg gaggttggtt tagaagttct ggaggctcta gctgaaaaaa caggttttga
    16201 ctatgagatt gacagacgac cgttcggagg tgcagatatt gatgcagcat ggcctccctt
    16261 acctgatgaa acccttaagg caagtaggga agcagatgct atcctactag cagctatcgg
    16321 tagtcctcag tatgatggag cagtggttcg ccctgaacaa ggcctgatgg ctctccgtaa
    16381 ggaactcaat ctttacgcta atattcgtcc tgtaaaaatc tttgacagtc taaagcattt
    16441 gtcaccactc aaactggaac gaattgctgg tgtagacttt gtcgtggtgc gtgaattgac
    16501 aggcgggatt tactttggag atcatattct tgaagagcgc aatgcgcgtg atatcaacga
    16561 ctatagctat gaggaagtgg agcggattat tcgcaaagcc tttgaaattg caagaaattg
    16621 cagaaaaatc cttactagta tcgataagca aaatgttcta gcgacctcaa aactctggcg
    16681 gaaagtagct gaggaagtcg cacaggattt ctcagatgta accttggaac accagctggt
    16741 agactcagct gctatgctta tgattaccaa tcctgctaag tttgatgtta ttgtaacgga
    16801 gaatcttttt ggagatattt tatctgatga atcaagcatc ttatctggta cacttggggt
    16861 tatgccatca gccagtcatt ctgaaaatgg accaagtctc tatgaaccta ttcacggttc
    16921 agcacctgat attgcaggtc aaggaattgc caatcctatt tccatgattt tatcagttgt
    16981 catgatgttg agagatagtt tcggacgtta tgaggatgca gagcgtatca aacgtgctgt
    17041 tgagacaagt ctggcggcag gaattttaac gagagatata ggaggtcagg cttcaacaaa
    17101 ggaaatgatg gaagctatta ttgcaaggtt atgaagttag acgaaaaaat tactctagtc
    17161 cttttgattt ggaatgtcat cattttcttg atttatggca ttgacaaatt caaggcaaga
    17221 agaagagctt ggcgcatcca agagaaaatc ttactcattt tagcccttac ttgtggtggt
    17281 tttggggcct ggttagctgg aatcactttt caccacaaga ctcgaaaatg gtactttaaa
    17341 acagtttggt ttcttgggat ggtgaccaca ctagtagcct tatattttat ttggaggtaa
    17401 tggatggcag ggtcttcgag ggaacacgct gcttgggctc tagcggacta tggttttaag
    17461 gtcgtgattg caggatcttt cggtgacatt cattacaata atgaactcaa taatgacctg
    17521 ttgccaatcg ttcagcctag agaggttaga gagaaactag cccagctaaa accaaccgac
    17581 caggtaactg tggacttgga acaacaaaaa atcatctcac cagttgaaga attcaccttc
    17641 gagatagata gcgagtggaa acataaactc ctaaatagtt tggatgatat cggtattacc
    17701 ttgcagtatg aagagttgat tgctgcttat gaaaaacaac gaccagccta ctggcaggat
    17761 tagaaaaaat agaaaaggaa atatagtaaa ctgaaataag atgtaaacaa atgaattaga
    17821 gcttaacatc catttccagc aattttttag aaactacagt ggactattct ggattcaaca
    17881 cattataaaa ttatgacaaa acacattcac aagaaggcta cgacatttta aaaggtgagg
    17941 gcggatgtat cgtttgccct actaaagttg gttacattat catgaccagt gacaaggcag
    18001 gacttgagcg taagttcgca gccaaagaac gtaagcgtaa caaaccaggt gttgttctct
    18061 gcggtagcat ggatgaactt cgcgctttag cgcaactcaa cccagaaatt gaagcattct
    18121 actaaaaaca ttgggatgaa gatattcttc ttggttgtat ccttccttgg aaaccagaag
    18181 cctctgaaaa actcaaagca tacggggatg gccgtgaaga acttatgact gatgtacgtg
    18241 gtactagctg ttttgttatc aagtttggaa aagcaggtga acaattggct gccaagcttt
    18301 gggaagaagg taaaatggtc tacgcctcat ctgcttcaat gacaaaacga ttgaaactcg
    18361 ctatgagagc aaggtgtaat ggtgtctatg gttgataagg acggcaaact catcccagaa
    18421 caaggaggag cacgttcaac ttcaccagca ccagttgtga tccgtaaagg gcttgacatt
    18481 gatatagtaa aatgaagtaa gagcaggaca aattaatcag gacagtcaaa tcgatttcta
    18541 acaatgtttt agaagtagag gtgtactatt ctagtttcaa tccactataa aatcatgatg
    18601 cacctgtcag atacttttaa ctcatgggac taccgtcagg ttgagtatta ttaggataga
    18661 gaagaagtct agtgttaagg acattacagc tcataatact aggctttttt atagaaattc
    18721 ttttcttttt ctataggata tggtattcta tgt
//
LOCUS       DAVKDZ010000037        18437 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000037 DAVKDZ010000000
VERSION     DAVKDZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18437
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.038"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(82..273)
                     /locus_tag="VWZ30_001269"
     CDS             complement(82..273)
                     /locus_tag="VWZ30_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEU9335002.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(434..1108)
                     /locus_tag="VWZ30_001270"
     CDS             complement(434..1108)
                     /locus_tag="VWZ30_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335003.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLTIRGDVAFGKL
                     EIVYVK"
     gene            complement(1114..1560)
                     /locus_tag="VWZ30_001271"
     CDS             complement(1114..1560)
                     /locus_tag="VWZ30_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9335004.1"
                     /translation="MKLRIEIDGNLEETEIVIRTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRFKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(1674..2177)
                     /locus_tag="VWZ30_001272"
     CDS             complement(1674..2177)
                     /locus_tag="VWZ30_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9335005.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLDQQVGVYVFIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(2174..2536)
                     /locus_tag="VWZ30_001273"
     CDS             complement(2174..2536)
                     /locus_tag="VWZ30_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HEU9335006.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKVESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGIRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(2602..4461)
                     /locus_tag="VWZ30_001274"
                     /pseudo
     CDS             complement(2602..4461)
                     /locus_tag="VWZ30_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(4500..4949)
                     /locus_tag="VWZ30_001275"
     CDS             complement(4500..4949)
                     /locus_tag="VWZ30_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9335007.1"
                     /translation="MDKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQAVRFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRMVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            complement(5073..5789)
                     /locus_tag="VWZ30_001276"
     CDS             complement(5073..5789)
                     /locus_tag="VWZ30_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9335008.1"
                     /translation="MGRKWSNIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHVIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(6670..8085)
                     /gene="ply"
                     /locus_tag="VWZ30_001277"
     CDS             complement(6670..8085)
                     /gene="ply"
                     /locus_tag="VWZ30_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEU9335009.1"
                     /translation="MANKAVNDFILAMDYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVL
                     TAKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKIEND"
     gene            complement(8096..8428)
                     /locus_tag="VWZ30_001278"
     CDS             complement(8096..8428)
                     /locus_tag="VWZ30_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335010.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEVLDHLVQPQLISLNKSEF
                     MQLNSGTY"
     gene            complement(8499..9107)
                     /locus_tag="VWZ30_001279"
     CDS             complement(8499..9107)
                     /locus_tag="VWZ30_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335011.1"
                     /translation="MFQSSYLLPLLWLKKEADKEKMSATQCQIFFFYYQLFELLFARE
                     SDLKDLCLGGQGFYFSQLEKDLLSGVSRFLTNLEGKGTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQAVERLETPLLLSFQDRQDLMWIYQSALEKDYSVKVIGDKHFVL
                     KRQDATKLTGRQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(9120..9578)
                     /locus_tag="VWZ30_001280"
     CDS             complement(9120..9578)
                     /locus_tag="VWZ30_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU9335012.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKEHWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASAEAPGKKE"
     gene            9703..10102
                     /locus_tag="VWZ30_001281"
                     /pseudo
     CDS             9703..10102
                     /locus_tag="VWZ30_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10287..11225)
                     /locus_tag="VWZ30_001282"
                     /pseudo
     CDS             complement(10287..11225)
                     /locus_tag="VWZ30_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(11761..12021)
                     /locus_tag="VWZ30_001283"
     CDS             complement(11761..12021)
                     /locus_tag="VWZ30_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335013.1"
                     /translation="MRERSATGAQGLSKFIKKHLNDITRLTASLLGDEKLSAITSSSA
                     VKADIHRFVIELEPVKSTIPQNDDISLDQNEIFQILKNFLDG"
     gene            complement(12143..12622)
                     /locus_tag="VWZ30_001284"
                     /pseudo
     CDS             complement(12143..12622)
                     /locus_tag="VWZ30_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12612..13607)
                     /locus_tag="VWZ30_001285"
     CDS             complement(12612..13607)
                     /locus_tag="VWZ30_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335014.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELALTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMNVVLVFSKLSDSEKKQLLQARIPFVDFKG
                     NLFFPTLGLVLNADDTEVPKELTPSEQLTWIAFLLTKDQKVVDVELLSRVTGLPNSTI
                     YRCLRTFKALDWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGTYALSHSTFLAETDENTSYVIWQRKFNQLSLPLSQHALEGKVLEIWKYCPF
                     ISEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRIEEESEVLENMILQYLGDDDAS"
     gene            complement(14016..14387)
                     /locus_tag="VWZ30_001286"
     CDS             complement(14016..14387)
                     /locus_tag="VWZ30_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335015.1"
                     /translation="MTEQLLEKLYSGKIVIPQEVYDEINIPTIPHLKSRIDQLVTKGS
                     AEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEAASISLAKKHNGILGSNNLRDVKPY
                     VEEFSLEHMTTGDILVEAFKA"
     gene            complement(14397..14780)
                     /locus_tag="VWZ30_001287"
     CDS             complement(14397..14780)
                     /locus_tag="VWZ30_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335016.1"
                     /translation="MYHLYYDEVKKSSVSLILIGEGDETERKADQFASYFLIFPSSLY
                     RMVEEIRENANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIE
                     TASRLGYDTSLYRPLSESKKETVLG"
     gene            complement(14994..15167)
                     /locus_tag="VWZ30_001288"
                     /pseudo
     CDS             complement(14994..15167)
                     /locus_tag="VWZ30_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055999.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(15532..16488)
                     /gene="lytA"
                     /locus_tag="VWZ30_001289"
     CDS             complement(15532..16488)
                     /gene="lytA"
                     /locus_tag="VWZ30_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU9335017.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKVNDTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIAEKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(16864..18234)
                     /locus_tag="VWZ30_001290"
                     /pseudo
     CDS             complement(16864..18234)
                     /locus_tag="VWZ30_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
BASE COUNT         5650 a         4008 c         3166 g         5613 t
ORIGIN      
        1 ccaggcacct aagttgccaa cgtagtaacc atcaggtgtg taggtattgc gaagcatctt
       61 acctgatgaa gccagataat actacttgcc attgtctttg acccaatcat tcgcaatcat
      121 ggaaccagaa gaacttacat aataccattc tcccttgtca taaacccaag tactgacttt
      181 catggttcct gagcaattaa aggcaaaaaa actgtccaat atcattcgtt ttttaaaagc
      241 atttgacact acatcatcta cgctgtagta catagataca tcaaacgtaa aagccaaaat
      301 atcgaaaaat atacaacttt taaaggcagc atttcatttt ttaaagtcta acaaccatag
      361 aactgaaatc agagcaaggc atcaatttcc tactcttagt acgtctattt tgatggttga
      421 agtgaagatt tttttattta acgtagacaa tttccaactt cccaaaagcc acatctccac
      481 ggatagtcaa ggttttgctg gttggggcta caggagcgtc agccttagct gcaccaaagg
      541 aggtttctac tttcaaatct acacgccagt gttgtggaac atagacggtt gcatttccga
      601 aggccacttc aatatttaaa gttgcaaaat cacctagcat ctctgcatta tcatagtaga
      661 ttttagcatc cccaaaagcg acttccactt ggtcatctac gagatcttga tcttgcttat
      721 agaaggtccc gctaccaaaa gcgacttcct tatccgtgac gactgttttt ttcccattgt
      781 accaccattt tttttcattc cagaacttac ttgaatgcgt cagataacca acaccaagta
      841 ccaccaagat gctagcccaa ataagagaat gattggtaac tggtaacaag tcataagcgt
      901 aatttgcaat gatgagcgcc agtaaacctg taaaaactgc cgaagtgaga tgacgtctaa
      961 ggatggactc aatggactta taagcaaaaa aaacaatacc tagtaaaggc catattttac
     1021 catccaaaga aggaattcca aaattccctt gcagcaagat ccaagctgct aaaaccaata
     1081 aaacaatacc aaatgctttc tttttcatgt tttttacctc atgtttctta gattttcttt
     1141 taggagggat tggtaatgcc gtgagacatg aacctccttg tgcgtctgat agaaggaaat
     1201 ggtgcccgtt cctgaaaagg acttgtccac tgagtaaatc tgacggatgt ttgcgatcgt
     1261 tgacttggaa actcgattaa aatagcgagg caagatagat tccaactcat agagcttgaa
     1321 gcgaacctca taagcttcct tctggttatg agcgtagatc ttgctccctt ctgtttcaaa
     1381 gaagagaatt tctgacaggt ctagataata ttcacctgtc cccttgtaaa aagtcaacct
     1441 cggagccttt gactcttgca agagccgttg caagtctgca atttcatctg tcaaagtggg
     1501 ggtcctgatg acaatttcag tctcctctaa attaccgtca atctcaattc gtaacttcat
     1561 cacatctcct ttctgactct actatatcaa agctaagtta ggaatacaag ggcaaaaaag
     1621 caagtggtca atttgaagca gtaagtggtt gtaggactag cttaacttac atgtcagaat
     1681 aggaagaaaa ggaatcccca caccagacca caagcatagc caaccaagac atccttggga
     1741 taatgaacac cccctaaaac cctcaccaac cccagcaaag ctgaaaagat caagcatact
     1801 aagccaatag gtagactagc atgcagacag gccatggaga tgatagttgc cgaaaagaca
     1861 tggcgactgg gcatcgaccg tcccgaacta tccttatcaa gaaggggaca gatatcccaa
     1921 gtttcataag gtctcgggtg attgattttc ttacgaaaca gggacaaggc cacaaaacct
     1981 gatgcaggaa taaacacata aaccccaact tgctgatcaa gtcctagttg caggtaagtg
     2041 gtgactagca agatcagata gaccaggggc atggcgactg tcatcaaacg attgaaactg
     2101 cgtaatagga ataaaagaat tggacgacta gttagtctgg agctgatatt tcgataccat
     2161 tcttgataat ttttcattta tgcttctcat acttttctag aacttgttga gatgttttcc
     2221 ccttggcaag ccaatccact agcttgtcca agcgacgtat ttcctgcatc agaggatcat
     2281 caatttcctc aatacggata ccgcaaatct tccctgtaat tgcaattcgt tcagggacat
     2341 aatatggtgc ttgtcgaaaa aaatctccgt aagtaacatc cctatccaga caagcaagaa
     2401 cgtcactcac ttcataccct gtcaaccaac tggtcacttg gtgaactgac tctacctttc
     2461 caccctttcg ctcaactttt gcaatcaagg ctaagtagac agcagcaaat ttcatattgt
     2521 ataatttctg actcatactt ctctctttct atgtgtacta gtataaatca gaaaaagaag
     2581 gccgtcaagc cttcttttga tttattcttc tgcttcatct tctgtaaatt gactattgta
     2641 caagtcagcg tagaagccac cttgcgccat cagttcctca tagttgcctt gctcgatgat
     2701 atttccatct ttcatgacca agatcaaatc tgcattttgg atggttgaca agcggtgggc
     2761 aatgacaaag gatgtgcgtc cttccatcaa acggtccatg gctttttgga tcaattcctc
     2821 tgtccgtgtg tcaacagaag aagtcgcctc atccaaaatc aaaagcggtc catccttaag
     2881 aagggcacga gcaatagtca atagttgttt ttgtcctaca gacaaggtca cggtgtcatc
     2941 caagatggta tcatagccat ctggcaaggt cataataaag tggtgaattc ccacagcctt
     3001 actagcttcc atcattcgtt catcactaat ccctatttga ttatagatga gattgtcccg
     3061 aatagttcct tcaaagagcc aggtatcctg caagaccatt gaaaaagcat catgcacttc
     3121 tgaacgcgtc atagccttgg tatccacacc atcaatgcga atacttccct tatcaatctc
     3181 atagaatttc atcaaaagat tgacaatggt tgtcttacca gccccagtcg gcccaacaat
     3241 ggcaaccttt tgaccagcat gagctgtcgc agagaagtca tagtcttgaa cattgacacc
     3301 gtccaccaga atttctcctg ctgacacgtc gtagaaacgt ggaatcagat tgaccagagt
     3361 tgatttacca gaacctgttg acccaataaa ggccactgtt tgaccagttt ctgctttaaa
     3421 gctaacatgt tcaataactg cctccgaatt tgccgcatag cggaaggtca catccttaaa
     3481 ctcgacctga cctttgaagt tttcatcagc cagctgcact tgaacagagt tttggataga
     3541 agaatgcaaa tctaaaactt gattaatccg cttagcagag accatagttc ggggaagaac
     3601 gatgaagagt gctcccatga gaaggaagcc catgacaacc tacatggcat aagacatgaa
     3661 aacaatcatg tcactaaaga gaggcagacg cgctatcgga gcaacgtcgt taatcacata
     3721 ggccccaatc cagtaaatcg ctacactcaa accacttgaa atccccatca tgataggatt
     3781 caaaatagcc ataagacggt tgacaaacaa attcaaacgg gtcaattcat catttactgc
     3841 tgcaaatttt tcattttgat aatcctctgc attgtagaca cgaacgacac gaatacctgt
     3901 taaactctca cgagtgatac tgttcagttt atctgtcagc ccctgaatca aggactgttt
     3961 tgaaaaggct agcgtcatca aaacggtcgt catcaggacg ttgacaatca ctgccacaag
     4021 tacggcccag agtcagtatt ctgaatgacc taaaatcttc ccaatagccc agatagccat
     4081 aattgaacca cgcgttacca cttgcaagcc catagtaatc aacatttgaa cttgagtaat
     4141 gtcattggta gtacgcgtca agaggctagg aattgaaaat ttcttaatct ttgtctgcga
     4201 gtaatccaaa actcggttaa aaatatcact tctcagccta ctaatataag aagccgccac
     4261 tcgggatgca aaaaatccaa ctgcaactac ggacaagaag gcaagaaagg gcattcccat
     4321 catcatgctt gccggctgcc acaactcatc taaattagtt ccttgactac ctagcaaatc
     4381 cgtaattttc gagatatagg tcggcacttc caactctaga tagaccgaaa agcaagtaaa
     4441 gagaatggct agtaaaatca tcccccattc ttttctacta attcttttgg ctaatttctt
     4501 tattctctcc tcctattccc ttgatatttt gcctgtagtt gaccgagaac tttctcaaaa
     4561 atcagtaatt catcttcatc aatgtcttcc atcaactgct tgtctatgcg ttcaaaaaaa
     4621 gccttaacct gttgcatctg agaacgtgct ttgtccgtca gacgaacaaa cttagcccgc
     4681 ttatcaacag gactcgcctc caattccacc aaaccatttt gcaccatacg cttaaccaga
     4741 ttactagcaa cagacttggt aatattgagt tcctgctcga tatctttaat caagaccaag
     4801 tcttggtttt tctcgcgatt atccaaaaaa cgcacagcct gaccttgcgg cccacccata
     4861 aattcaatgc cgcaacgttt ggcttccttt tgcaccatca ggtgaatttg atgaccaaaa
     4921 cgcttaaaga ctaacatcgg tttatccata atctccccct tctaaataaa aatagttctc
     4981 tggagaataa ttaaatttct atgagaacta ttttcttgat taaaaaaatc ccaagtgatt
     5041 ttctcactta ggatcatgtt ctataggtta aattaaaacc catctacgtt cgtataaatc
     5101 ttttggacgt cttcgtcgtc ttcaagaacg ctgtaaagtt tttcaaaggt ttcaaggtct
     5161 tcgcctgaca attccacttc tgactgagga atcatttcca attcagtcac ttggaattct
     5221 tcaataccag actcacggag ggcaacgata gccttgtgaa ggtcagttgg cgctgtgtaa
     5281 actgtgattg taccttcttg tgcttctacg tcatccacat ccacatccgc ttcgagcaat
     5341 tgctcaaaga ctgcgtccgc atcttcacct ccaaatacaa taacaccttt gttgtcaaag
     5401 aggtaagaaa cagaacctga agcgcccatg tttccgccgt ttttaccaaa ggctgcacgg
     5461 acattggctg ctgtacggtt aacgttagaa gtcaaagtat ccacaattag catagagcca
     5521 tttggcccaa aaccttcgta acgtccttct gtaaaggttt cgtctgtgtt tcctttggct
     5581 ttatcaatcg ctttatcgat aacgtgtttt ggcacttggg cttgtttagc acggtcgata
     5641 acgaatttca aagctgagtt tgattctgga tctggatcac cttttttagc tgctacatag
     5701 atttctacac caaattttgc atatacttta gagttggctc catctttagc cgttttcttg
     5761 gctacgatat tggaccattt acgtcccatt aggaatctcc ttttttcaca ttttaatctt
     5821 tcttattata acacaagttt ttttgatttt cactagagga aatgggtttt attagcaaat
     5881 caagctagga tagtacttta cctgctaaga tggtcttgcc tttctatctt tatcaacaga
     5941 cactcatcca cattcaaaaa acaaactaga ccattatctg caaatagaaa gtttcagcca
     6001 agtttgacaa agtcagctca aattactgtt tgaagtttgt agatataagc gacaaaaaca
     6061 atcatactgc accttttgtt gacagtctac tccagacata tcatagttca agtaaatact
     6121 ttgaaatcca acagttctta taggtgctat tgtattctaa gaaatcaata gaagagtttc
     6181 taagcaaatc tctaatactc aataaaaatc aaagagcaaa ctagaaagct agccgcaggt
     6241 tgctcaaaac actgttttga agttgcggat agggctgaca tggtttgaag agattttcga
     6301 agagtataat ttacgtgttc ccaagatgga gaagttagac tagtacactg gcacttctaa
     6361 accattgcta gcaattgatt tgttcatatt taatttcatt ttttccataa atgggtatta
     6421 gatataaaca gcaaaatatt tccgatacgt gtcattcttg aatttccaat catctaaaac
     6481 aagtaaagga taatcaatcc cctgtatatc aaggaattgg ctaccctttt tactttttta
     6541 cacattctgt ttgatagatt cattttaaca tcacgagcat actccaatgg aaatcgctag
     6601 gcaagagata aactttcaga tatccgcaga gagatcatcg cctctttttg tcgcaagcat
     6661 tctcctctcc taatcatttt ctatcttatc ctctacctga ggataaagag tcgttcccca
     6721 aatagaaatc gtccgcttac gcactagtgg caaatcggtt ttttcataaa ccgtacgcca
     6781 ccattcccag gcaagcccgg tacactctct aattttgaca gagagattac gaacattccc
     6841 ttttaaagga atactagtgg taaagtgagc cgtcaaatcc tgcccatttc tgtcccaagc
     6901 cttagcagtt aagacttcct taccttgatg atcataggat aattcatccc aagtaatata
     6961 atattgggca acataggcac cactatgatc cagcagtaaa tctccgtttc tgtaagctgt
     7021 aaccttagtc tcaacatagt ctgtactgtt ttgaaaggtc gcaactacat tgtcacgtaa
     7081 aaaagaagtt gtataggaaa tcggcaagcc tggatgatct gctgtaaagc gactgccttc
     7141 ttgaatcaag tcctctacca tatccacctt gcctgttaca actcgggcac ccgaactcgg
     7201 gtctccccct aaaataaccg ccttcacttc tgtattgtcc aaaatctgct tccactctgt
     7261 ctgaggagct accttgactc cttttatcaa agcttcaaaa gcagcctcta cttcatcact
     7321 cttactcgtg gtttccaact tgagatagac ttggcgccca taagcaacac tcgaaatata
     7381 gaccaaagga cgctctgcag aaattcctct ctgctttaaa tcctctaccg ttacagtatc
     7441 ttgaaacaca tctcctggat ttttaacagc atctacgctg actgtataat aaatctgctt
     7501 aaaattaaca atctgaatct gcttttcgcc tgaatggaca gagttaaaat caatatcaag
     7561 agaattccct gtcttttcaa agtcagaacc aaacttgacc ttgagttgtt ccatgctgtg
     7621 agccgtgatt ttttcatact gcattctagc tgggacatta ttgacctgac cataatcttg
     7681 atgccactta gccaacaaat cgtttaccgc tccgcgaaca cttgaattgc tggggtcttc
     7741 cacttggaga aagctatcgc tacttgccaa accaggcaaa tcaatactat aagtcatcgg
     7801 agcacgatcg accgcaagaa gagtgggatt attctctaac aaggtctcat ccactacgag
     7861 aagtgctcca ggatagaggc gactgtcgtt ggtagctgtt acagaaatat cacttgtatt
     7921 tgtcgacaag ctccgcttct ttctttcgat aacaacaaac tcatcgggta gctgattacc
     7981 ctctttgatg aaacgatttt caatactttc tccctgatgg gtcaagagtt tctttttgtc
     8041 gtaatccata gctagtataa agtcatttac tgctttattt gccatcttct acctcctaat
     8101 aagttcctga attgagttgc ataaactcag acttgttcag cgaaatcagc tgtggttgga
     8161 ctaggtgatc caaaacttcc tcgtacaatt cttctgagac attgcgtcgc cgtctggcta
     8221 gataagaagt cggaatgacc gtattatcca acataaatac cttatctaag tcaatcaagg
     8281 ttggtcttgt aaaaggatta cgagctagat ccggctcttc tatcataaag ttcttgacca
     8341 aacgtctggt caagagagct ggtttgaagg tctgattttt aaccaactct ttgtttttag
     8401 tcatgctgtt gtcaatacag atatacatat gattcttcac agccaaatcg ctactaatac
     8461 ttggaaaagg caaataaaga gctacaacat ctcctctctt aatcaagcaa gagcaccccc
     8521 ttttctccta atgtaacata gacaggattg accaagtctt ctgattgact cagaatttcc
     8581 aaagtttgag tttggcgccc tgtcaattta gtagcatctt gtctcttcaa tacaaaatgc
     8641 ttgtcgccaa tcaccttgac actataatct ttctccaaag ctgattggta aatccacatc
     8701 agatcttgtc tgtcttgaaa actcaagagt aagggcgttt caagtctctc tacagcctga
     8761 taaaaatcaa aaacaggagc taattcctgc caatctgact ggctagttgt caaggctaga
     8821 aaaagggctt tgcgagctga tacttcttgg ttagccttga gagtcccttt tccctccaag
     8881 tttgttagaa atcgggaaac tccagaaagc aaatctttct ctaactgcga gaaataaaaa
     8941 ccttgccctc ccagacacaa gtctttcaag tcgctttctc tagcaaataa gagttcaaac
     9001 agttgataat aaaagaaaaa tatctggcac tgggtcgcgc tcatcttttc cttatcggct
     9061 tcttttttta accagagcaa gggcaatagg tagctggatt gaaacatttc ctccacctcc
     9121 tactcttttt tccctggagc ttctgcacta gctgccactt cttttgactg gatactttcc
     9181 cactggttga tttcctctga gataagacct tcgcatgtct tgacaaatag ggcaaaagcc
     9241 ttggtctttc ctgcatattt ctccgtttgg cattgataga ggaatttttc tttctccagg
     9301 agttgcgcag tcttttggta agaaatccag tgttccttgg cattatacaa attgataatc
     9361 ccctcacaca gcaagccgag actggataag gcaaccgaaa tcaaacggta gcgatcacct
     9421 ggcataggaa tagcacaaaa gacagctatg aggaaacctg ccacgatttc tgttattttt
     9481 aataccttat agcgtctacg atgttgaacg cttttcttta aaaaatgagc tatctgtacg
     9541 tctaatcgct ctgtcaggta catttcttct ggcgtcatgt tcgtaactcc tttcatttac
     9601 tttgataatc atagtataac atatttgaat atcaaagtat agtaaaaact ggactataac
     9661 ttattagact tcctgcgaaa caaaatatgg tatagtagtt ctatgcatta tgaagcaagt
     9721 aaacaactaa ctgatgcacg atttaagcgt cttgttggtg ttcagcgcac gacttttgaa
     9781 gagatgttag ctgtattaaa aacagcttat caaaaaagtc ggacaagctg gtaaaatctt
     9841 ggctgacagt ggttatcaag ggctcatgaa gatatatcct caagcacaaa ctccacgtaa
     9901 atccagcaaa ctcaagccgc taacagctga agataaagcc tataaccatg cactatctaa
     9961 ggagagaagc aaggttgaga acatctttgc caaagtaaaa acgtttaaaa tgttttcaac
    10021 aacctatcga aatcatcata aacgcttcgg attacgaatg aatttgattg ctggtattat
    10081 caatcatgaa ctagaattct agttttgcag gaagtctatt atatacatgc acagacatat
    10141 gaaatctttt tgatacagcc ttctttttac atagagagac agcaattatg atatatttat
    10201 ccatgcacaa catcttctga gcctgggaca aaatagttct cgaccacaaa aaagctagag
    10261 atttccaatt gtggaactct agctttttaa ttttgagccg ttctcttatt gtattttagg
    10321 agattattga ccataagtac caatcccatg tcaattctca cttgacgctt ccctctcaga
    10381 ttacatctct tgtaacccaa agaagccttt atctgcccaa agacaggttc cacatcaatc
    10441 ttgcgttgag cgaaaatctg tctaccttgg ggagataaaa gcgcctgaca ttctttaact
    10501 ttcaagtttt gatagccttt gttcatatac agtccctttt gaggggctga ttcaggttcg
    10561 tcggtgtagt aaatcttgat ttcttgttga aagtctgtct gtgttttctg atgtttggta
    10621 tggtgaaaac gataatacca gccatcagga tgtgtgtagc tatcctcctt gttatcatag
    10681 tgccaatttg ctaagtttct agctgactgt ttatagcctc tcttctgttc cttatcaaac
    10741 atggcatatt taatcagatg gtttacctcc ttttcatcta aacgaaggag gttctcttca
    10801 cttccctatc cagcatctgc ggcaactgtc ttcaagtcat gcggataggt ttcaaggaat
    10861 ggcagaagag tcttggtgtc tgtcggattt gaaaagacat catagtgaag aacaaattgg
    10921 ttttccgtag cgatttgaag attataagca gccttgagtt gaccattttt catatggtct
    10981 tctttcatcc gcataaaagt ggcatctgga tcggttttgg aaaaactgtt acgcccttca
    11041 aatgtctcct gatagttctc atatttttca gcacgtattg aaaaatcctc cttgacttta
    11101 cgcaagactt tcttgagttt ccgacgttgg gttttacgct catccttccc tttaacgggt
    11161 gtctcctcaa tgttctggtt caggttttcc aattcttctt caagaacttg agcgaatgca
    11221 agcaacggca aatccgttgc tttttttgga gataagagac tattgtccca gactcatttt
    11281 aattacacaa tcgtttcaaa aaatatgatg gaagcctgag tattttatag taattccctt
    11341 cctatcaact aggtcaacca gttgagtgtc acaccctatc ctcagcaaag gactattgcc
    11401 accatttcct agccgatagg aggctccaaa ccacctattc taatactcaa gagcaaacta
    11461 ggaagccagt cgcaggctgc tcaaaatact attttgaggt tgcagatgga agctgacctt
    11521 gtttgaagag attttcgaaa agtataaaaa ccacctgaaa caaatcaagt gactaaagga
    11581 taaaaaacaa agtctgggca aaaagttttg aaaatcaaaa aacgcataat atcaggtgtt
    11641 gaaaaccttg atactatacg tttgattgtg ggaagattga cttcattttc tcctgaaatt
    11701 gagttttgct cagtttccag agatagacga attcaatata actatcttgc tacaattatt
    11761 ttaaccatcg agaaaatttt tcagaatttg aaaaatttca ttttgatcca atgaaatgtc
    11821 atcattttga ggaatagttg acttcacagg ctctaattct atcacaaaac ggtgtatgtc
    11881 tgcttttacc gcactacttg atgttatagc cgataacttt tcatctccta gcaaggaagc
    11941 tgtcaaacgg gtaatgtcat tcaaatgctt tttaatgaac ttacttaaac cttgagcacc
    12001 tgtagcagag cgttccctca tatccagcca agctttagcc ttaaaaataa ttaatactca
    12061 atgaaaatca aagagcaaac taggaagcta gccgcaggct gtacttgagt acggcaaggt
    12121 gaagctgacg tggtttgaat ttgattttcg aagagtatag accacaatta ctcaataccg
    12181 aatacccctg aatgatctct ttttcatgca ccaatatatt ataataatcc tcatccaata
    12241 ataaggctga taaactagca tcttggtcaa aatgtaaggg aatttctcga ccatccttct
    12301 ttaatggata ttgtggtaag atactaaatg gttcaatcat agaaggaaac tcaggtttag
    12361 ttgttgtaaa tcgaaaaagc tgcgctttct catctttctg acttccttga tactctccca
    12421 attctaaaaa atgattcaag gtattataaa attccttatt ttttagttca tcaatgatga
    12481 ccatatcata atcttttgtt gtgcgacttt taaatccttg cgaatccaat acgatagagg
    12541 tagcagttcc cccaatcaga acataatagt ccttaaaatc cgcaaacatt tcttgaaaaa
    12601 tagctttcat attagctggc atcatcgtct cccagatact gtaatatcat attttctagt
    12661 acttcactct cttcctctat acgtgggtca tcatcatctt ttaaggtcaa ataaagagaa
    12721 atcggatcta caaattgttt atcatgatta tttttaaaat cattccaaaa ctcagatata
    12781 aaaggacaat atttccatat ctctaacacc tttccttcta aagcatgctg agaaagtggc
    12841 aaggataact gattgaattt tctctgccat atgacatagc tagtattttc atccgtttca
    12901 gctaaaaaag ttgaatgcga caaagcataa gtaccaccat atagaaggtt agaaacagat
    12961 tttatctgct ttatatcgcc atctggcaat aaaatccgtt ttttgatggg attaaataaa
    13021 catgacacgg attttaagaa taattctttc tttgacaccg tatatgtgta aagcttattt
    13081 tgcttgttta accaatctaa agctttaaaa gtcctcaaac acctataaat tgttgagttt
    13141 ggaagtccag tcacccgtga aagcaactca acatctacta ctttttgatc ttttgtcaat
    13201 aaaaaggcaa tccatgttaa ttgttcgcta ggtgttaatt ccttagggac ttcagtatca
    13261 tccgcattca gtactagtcc caatgtaggg aagaagaggt ttcccttgaa gtcgacaaaa
    13321 gggattctag cttgaagtaa ttgctttttt tcgctgtctg ataactttga aaacaccaaa
    13381 acaacattca tattggcttt ttcacccata gtacgagcct gagtaacaaa tgaacttaaa
    13441 ctcccccttc tcttttcttt aatcaataaa aatgattcct tatgaaaagt catttgagta
    13501 tactcaaacc tttttacaaa actgattggt aaagtcaaag ccagttcctc aatactgaca
    13561 tcaacataaa aggattgaaa aatcgacatt atctttttga cattcatggt ttttccttat
    13621 tctatcattt ttattacatt ttatcattta gtatgataaa atacaaggtt tttgataaaa
    13681 taaccaaaaa ctactcgcta tcccctctta catcacccta catatcgttt aacatacgac
    13741 tgatagttca ggggaaactt caaggacagc ttttttctca tccactatgc aggacttact
    13801 atttcacttc tactcctata ggctcaattt gacacttttc tttttcgaat tgctcatatg
    13861 cctcctgaat agtgagttcg atttatccaa atttattttt gtctattttg atgaatactt
    13921 ccacgaagat agtctgaaaa tgaatttgca ccaattttcc ttctcttttt aagcatatta
    13981 ttccagatat gattgccctc ttgttcagta ataaattacg ctttaaacgc ttcaaccagt
    14041 atatctcctg ttgtcatatg ttctaaagaa aattcttcta catatggttt aacatctctt
    14101 aggttattac ttcctaatat cccattatgc tttttcgcta aggaaataga agcagcttct
    14161 cccttaccaa taatcttgtt actatcatga tttcttgtta aatctctata taatgcgtat
    14221 tcttcagttc caatgtctat gctcacaatc tcagctgaac ccttagttac caactggtct
    14281 atcctagatt ttaaatgggg aattgtaggt atattgattt catcatacac ctcttgtgga
    14341 ataacaattt tacccgaata gagcttttct aaaagttgtt cagtcagatt gatatattat
    14401 cctaataccg tttccttttt actttctgac aaaggacgat ataaacttgt atcatagcct
    14461 aatcttgaag ctgtctctat aacactaata tccatatttt taatttcttc tgcatcaagg
    14521 tatccatcat tcctcaatct atataacata gctttatgac tgataccata aaactgaccc
    14581 aattttataa tatcttctac ttcaagatga gttctattgg cattttctct gatttcctca
    14641 accatcctat acagtgaaga tgggaaaatt aaaaaataag aagcaaactg atccgctttt
    14701 ctttcagttt catctccttc accaatcaag ataagactga ctgaactctt cttcacctca
    14761 tcataataaa gatgatacag ttcatgtgct aaagaaaatc tttaccttcc taataaatgt
    14821 ctgaattgat tgcaatgaga ctgaactgag ttcctttata acagactccg ctaatattct
    14881 ttccgagtcc ataaaatacc agcgtcaaat tttatatctt ttgtaccaat ttaaaaatat
    14941 ctatcggcga ttcaccatca gctcccaact tttttctaag atttgaagct ttattactta
    15001 aatccattcg tgatattcct ttcacctttt tccattttgg caatcatgct ttgattcaaa
    15061 gacaaatact cagcaatctt atcttgagta agatttgaag atattctaag ttcttttatt
    15121 cttttccaac atcacataca tttatcacat acatttatca tattcataca cctctcataa
    15181 aaagaatagc acactcttgt cataattttt taaataaaaa aattatgaca aaataaggaa
    15241 gcaatttatt gatgctgctt aaaaatctaa aattgatgat attaaaccta tttgatgagt
    15301 tcctatcaaa aatcgtatct tcaacctcaa aacagtactt aaagctatcc gactcggttt
    15361 acattgtcaa atttagattt tatttgagca taactttcta gtttgctttc tgatttttgt
    15421 ttaatatagt agcaaacatt gaaggaaata tctccacaag aaaacgcata ctattaagct
    15481 ttttcaagac ctaataatat gcgctgttct gatttgaaag acattccatt attattttac
    15541 tgtaatcaag ccatctggct ctactgtgaa ttctggcttg tctgccagtg ttccgtctgg
    15601 tttgaggtag taccaacctg ttccgtccgc tgactggata aaggcatttg ataccatggc
    15661 gccttcttta gcgtctaagt agtaccaagt gtccttgtac ttgacccagc ctgtcttcat
    15721 ggcaccttct tcgttgaaat agtaccactt ctcagcgatt ttcttccagc ctgtggccat
    15781 ttcgcctgag ttgtcgaacc agtaccagtt gccgtctgtg tgcttcctcc agcggtctgc
    15841 aagcatatag cctgaactgt caaagtagta ccaagtgtcg ttgaccttct caaacttgtc
    15901 ttttggataa gagccgtctg aatgtacgta ccagtagcca gtgtcattct tctgccagcc
    15961 tgtttcaatc gtcaagccgt tctcaatatc atgcttaaac tgctcacggc taatgcccca
    16021 tttagcaaga tatggataag ggtcaacgtg gtctgagtgg ttgtttggtt ggttattcgt
    16081 gcaatactcg tgcgttttaa ttccagctaa actccctgta tcaagcgttt tcggcaaacc
    16141 tgcttcatct gctagattgc gtaagagttc gatataaagg cggtagtctg tcatgaactc
    16201 ttctttagtt gaatggcttt caatcagttc aaccgctgca taggtctcag cattccaacc
    16261 gcccccaacg tcccaggcac cattatcaac aggtcctacc tgcatgatgc aaccgttccc
    16321 aacaatgtgc gagaaaaaac ctaattctgg gtctttccgc cagtgataat ccgcttcatt
    16381 ctgtacggtt gaatgcggat tcccagttga gtgtgcgtgt acttgcctat atggttgcac
    16441 gccgacttga ggcaaatctg ttcttaattt actcacatta atttccatat tctactcctt
    16501 atcaattaaa acaactcatt ttttacaatc cagaaccaga aactccttta tttctacctt
    16561 acaaagaaga caatcttagt cacgattagg cttgtagata gaacctcaaa acgcactatt
    16621 ttgacactgt aaataggact gacaaggtct gcattctatc tacaataaca ccccagacta
    16681 aaaagctttt caaaagtata tttttacagt ctctatatgt ccttttcata aatgctatac
    16741 tttattatat catataaaag aagtcaaaag tctgttaaac tattttcaac accaaactaa
    16801 agaggagaac acaagttttt tcgattctca ctagaggaaa tggattttat tcagtaaatc
    16861 caactaggat tgcactttgg ttgccaagat tgccttgcct tcttttatca agggatgaca
    16921 gaatagtgag aagtacagtt gaactgtcat ggcaacccag ataagaggtt cacaaaggat
    16981 aacaccctta tatcctgccc aatgaataat caaaaccaca aaaacgattt ttccgattag
    17041 ttcaataaag ctagaaacta gaggaaggat cttttgcccc aagccctgca agcaattgcg
    17101 ataaatcaac aagaggctca aaatgggata aaaggctgaa ctgatttgca gatagagact
    17161 tccattttct atcaagtaac catctgtcga actagccaag aaggaaacca aggctggact
    17221 ggcaaaaaag aggaaaatac aaacaaaaac tgcccaggat atacttaaac gactaccgat
    17281 tcgaagacct tgagcaatgc ggtcaggtcg cttagctcct cgattctgag aagcaaaggt
    17341 agtcattgat gcagaaatag cggtcatagg aagaagggca aagaccataa tgcgtcgagc
    17401 tgccgtctgg gcactaataa tcactgcacc aaaggtatta acagaagact gtaaaattac
    17461 actgccgata gatacaattg aactcatcaa gcccatagcc aaaccttgct ccaagagatc
    17521 cgcgtacaag ctttcgtccc atttgaaatg tttaaactgt ggcaagagtt ctggcacact
    17581 tttacgaata taataaaagc agagaaccgc tgataaacct tgcgaaataa tggtagcaag
    17641 tcctgcagat tgaactccca gatgcaattg cgtaataaaa tagagatcta gaaccacatt
    17701 aaccaagtca gagaaaatca gaaatcccag ggctgctaga ctgtcaccaa tagaccgcaa
    17761 caagcctgta aaaagattat aagcaaagct gacacctaca caggtcacaa tcatagaaat
    17821 atattgataa gattgaggaa gaatttctgc aggagtatct aagtattgca agagaggata
    17881 caagccaaga aagcccagca acataactag aatgctcaaa agagcaccta aaatctaagt
    17941 ggctgctact acttccttga ttttagtgaa attccgagcc ccataataac gagcaatgac
    18001 aatccccatg ccattgccaa caccaagtgt aaaacctaca atcaggtcaa aaatcgctgt
    18061 cgtcgctcct actgcagcca aggattcttg accaagaaat cgtccaacaa tcaagacatc
    18121 agcagtgtta tagagctgtt gaaaaatatt tgatagcaag attgggaagg tgaagcttaa
    18181 gaacgaggga agaatcgaac catgtatcag gtccactgtt cgttgtttat tcataaagct
    18241 attatatcaa ctttctagag gagcgacaag aaacagcaaa ctatatatag caaaatgaaa
    18301 taaaaacagg ataaatcaat caggacagtc aaatcgattt ttaacaatgt tttagaagta
    18361 gatgtgtact attctagttt cgatctatta tagttggttg agtttggaat agtaagctgt
    18421 agctgctaaa atatttc
//
LOCUS       DAVKDZ010000038        17772 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000038 DAVKDZ010000000
VERSION     DAVKDZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17772
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.039"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            166..642
                     /locus_tag="VWZ30_001291"
     CDS             166..642
                     /locus_tag="VWZ30_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9335018.1"
                     /translation="MGLDHFFDKNLVFCLEADNQEHLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVYNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEPLYAYIAEAIA"
     gene            681..986
                     /locus_tag="VWZ30_001292"
     CDS             681..986
                     /locus_tag="VWZ30_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9335019.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGFDVHCDAVMVKDVN
                     EDLIKGYDIFTPIAATDLGFDPGIPVIEAGLILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            1034..2509
                     /locus_tag="VWZ30_001293"
                     /pseudo
     CDS             1034..2509
                     /locus_tag="VWZ30_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
     gene            2535..2612
                     /locus_tag="VWZ30_001294"
                     /pseudo
     CDS             2535..2612
                     /locus_tag="VWZ30_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2760..3656
                     /locus_tag="VWZ30_001295"
     CDS             2760..3656
                     /locus_tag="VWZ30_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEU9335020.1"
                     /translation="MVIELKSEQLSVQFNSFGGALSSIKDAEGLEYLWQGDATYWSGQ
                     APVLFPICGSLREDTALYIDEKGQESKGKIPRHGLVRKKEFELVEQTDKSVTFAIEDD
                     ENSYQNYPYHFRLEITYSLTGKTVRTQYKIFNKETSKVMPYFIGGHPGFNCPLLDDEV
                     YEDYYLEFEKEETCSVPRPFPETGMLDFQDRSPWLEGQKEIDLSYDLFSTDAVTLDEL
                     QSRTIALRSRKHDKGVKVHFAEFPNLIIWSTLNKGPFIAFEPWSGLSTSLEEGDHLED
                     KKNVRLLEANQVEEIGFEIEVL"
     gene            3814..4239
                     /gene="lacA"
                     /locus_tag="VWZ30_001296"
     CDS             3814..4239
                     /gene="lacA"
                     /locus_tag="VWZ30_001296"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEU9335021.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGRDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            4270..4785
                     /gene="lacB"
                     /locus_tag="VWZ30_001297"
     CDS             4270..4785
                     /gene="lacB"
                     /locus_tag="VWZ30_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEU9335022.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPTEENKKLIAKIEHVESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            4796..5725
                     /locus_tag="VWZ30_001298"
     CDS             4796..5725
                     /locus_tag="VWZ30_001298"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEU9335023.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTINRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIKGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQEFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            5727..6707
                     /gene="lacD"
                     /locus_tag="VWZ30_001299"
     CDS             5727..6707
                     /gene="lacD"
                     /locus_tag="VWZ30_001299"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEU9335024.1"
                     /translation="MALTEQKHVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQRTATSWKERV"
     gene            6966..7583
                     /locus_tag="VWZ30_001300"
     CDS             6966..7583
                     /locus_tag="VWZ30_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335025.1"
                     /translation="MATKKSFKALVVGLGIVSIFSSALPMVSGSVFADSALTTVDKAN
                     DIVLNVDGNKFYNVSVSEDIVNAGQILEDYFYVDKFGNINLKGTPEELSKNIGISVQE
                     ASLMYEAVKELPNVYERGPVGFRFNLGPQVRGMGGWAAGAFATGYAGWHLKQFAVNPV
                     TSGFVAVISGAIGWAVKTAVENYWTVAVATVEVPFVNLVYTIDLP"
     gene            7888..8724
                     /locus_tag="VWZ30_001301"
     CDS             7888..8724
                     /locus_tag="VWZ30_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9335026.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIIIGKGIAFGKRKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLKGYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            8760..9077
                     /locus_tag="VWZ30_001302"
     CDS             8760..9077
                     /locus_tag="VWZ30_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9335027.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            9077..10768
                     /locus_tag="VWZ30_001303"
     CDS             9077..10768
                     /locus_tag="VWZ30_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9335028.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIENGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VEAAQNTITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVVLAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            10940..12346
                     /gene="lacG"
                     /locus_tag="VWZ30_001304"
     CDS             10940..12346
                     /gene="lacG"
                     /locus_tag="VWZ30_001304"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9335029.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPLDHENPADVRAAELEDIIHNKFILDATYLGHYSEKTLAGVEAILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGKKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12389..12637
                     /locus_tag="VWZ30_001305"
     CDS             12389..12637
                     /locus_tag="VWZ30_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEU9335030.1"
                     /translation="MFKDFIQSIYEKVYIINFDKCSQIPCLTNEELKSLGKWYVYTGK
                     EWICHSDYELEEFKNIFLNFINPEEWDTISFDSDFMPF"
     gene            complement(12929..13690)
                     /locus_tag="VWZ30_001306"
     CDS             complement(12929..13690)
                     /locus_tag="VWZ30_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9335031.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIARFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFELSKLTKVIKP"
     gene            13869..13955
                     /locus_tag="VWZ30_001307"
                     /pseudo
     CDS             13869..13955
                     /locus_tag="VWZ30_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(14024..14986)
                     /gene="nrdF"
                     /locus_tag="VWZ30_001308"
     CDS             complement(14024..14986)
                     /gene="nrdF"
                     /locus_tag="VWZ30_001308"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEU9335032.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(15174..17333)
                     /gene="nrdE"
                     /locus_tag="VWZ30_001309"
     CDS             complement(15174..17333)
                     /gene="nrdE"
                     /locus_tag="VWZ30_001309"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEU9335033.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(17414..17632)
                     /gene="nrdH"
                     /locus_tag="VWZ30_001310"
     CDS             complement(17414..17632)
                     /gene="nrdH"
                     /locus_tag="VWZ30_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEU9335034.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
BASE COUNT         5478 a         3144 c         3659 g         5491 t
ORIGIN      
        1 aaaaaatgct ggtagtattt gtttgtgtgt ttatttttat ataacaaact acaaacgaaa
       61 caaaaatata aaaaaagaga caaaaatcaa cagaaaacta ttgacaacgg tttcatatag
      121 tgttatactt atggcataaa gttaatggaa agaagggaaa actttatggg attagatcat
      181 ttctttgaca aaaaccttgt gttttgctta gaagcggata atcaagaaca tctctttgat
      241 caggttgcaa ctttattgga agaacgagaa attgtaactc caacttatcg tgaagccttg
      301 atcacgcgtg aaaagtcatt tccaactggc ttagatatgg aatttttggg taaggatttg
      361 ccgaatgtgg ctattcctca tacagatatt gtttataatc tagctgaaaa agttgtggtc
      421 gttcgattag agaaaccggt gactttccac aatatgattg cgccagataa ggaagtagaa
      481 gtatccttgc tcttctttat catcaataac tcaagttcaa gtcaaaccaa cattcttgct
      541 cagttgatgg acttctttac tggaaatggt catcttgaag acctatcaaa aatttctgaa
      601 ccagaacccc tctatgctta tatagctgaa gcaatcgctt aatcttgttt attaaaaata
      661 ttaaatcgga ggaaatccat atgattaaaa ttcttgctgc ctgcggtgca ggtgttaact
      721 caagtcacca aattaaaagt gctcttgaag aagaactttc aaaccgtggt tttgatgttc
      781 actgtgatgc ggtaatggtc aaagacgtta atgaagatct tatcaaaggt tatgacatct
      841 tcacaccaat tgctgcgaca gaccttggtt ttgacccagg tattccagtt atcgaagctg
      901 gactaatctt attccgtatc ccagcaatga gtgctccagt atttgacaat attgaagcag
      961 ctatcaaaga acacgggtta agctaatttt aatttgttaa cattcatata acataaaaaa
     1021 gggaggaact gttatgtatg caatctttga cctaatcgga aaggttttca atcccatctt
     1081 agatatgggt gggcctgtca tcatgttaat cattttgaca gtattggctt tactttttgg
     1141 agtgaaattc tccaaagcgc ttgaaggtgg tatcaaactt gccatcgctc ttacaggtat
     1201 cggtgccatc atcggtatgc ttaacactgc tttctcagcg tcgcttgcaa aattcgttga
     1261 aaacactggt atccaattga gtatcactga cgttggttgg gcaccacttg ctacaatcac
     1321 ttggggttct gcttggacac tatacttctt gctcatcatg ttgattgtca acatagtgat
     1381 gctagctatg aagaaaacag atacacttga cgttgatatc ttcgatatct gacacttgtc
     1441 tatcacaggt cttttgatta aatggtatgc agataacaac ggtgtgagtc aaggggtttc
     1501 actctttatt gctacagcag ctatcgtcct tgttggtgtg ttgaaaatta tcaactctga
     1561 cttgatgaaa cctacatttg atgaccttct taacgctcca agttcatcac caatgacatc
     1621 aactcacatg aactacatga tgaacccagt tatcatggtt ttggataaga tttttgaaaa
     1681 attcttccca ggtcttgata aatacgactt tgacgctgca aaattgaaca agaaaatcgg
     1741 tttctgggat tctaaattct ttatcggttt catccttggt atcgttatcg gtattatggg
     1801 aactccacat ccaattgcag gagttgaaga tgcagataaa tggcgtcttg ttatcagagg
     1861 atggttgtct cttggtttga ctgccggtgt atctttggaa ctcttctcac ttatcggttc
     1921 atggttcatt gcagccgtag aacctctttc acaaggtatt acaaacgttg ctactaaacg
     1981 tcttcaagga cgtaaattca acatcggtct tgactggcct ttcatcgctg gtcgtgctga
     2041 aatctgggct tgtgccaacg tacttgctcc aatcatgttg attgaagctg ttcttctttc
     2101 aaaagtcgga aatggtatct tgccacttgc aggtatcatc gctatgggtg ttactccagc
     2161 tctcttagtt gtaactcgtg gtaaattgct ccgtatgatt atcttcggaa cactcttgtt
     2221 gccactcttc cttctttcag gtacacttat tgcaccattt gcaacagaac ttgctaaagg
     2281 tgtaggtgcc ttcccagcag gtgtgagcca aactcaattg atcactcact caactcttga
     2341 aggaccaatc gaaaaacttc ttggttggac aattggtaac actacaactg gtgatatcaa
     2401 agcaatcctt ggtgctgtag tcttccttgt attctatatc ggtatctttg cttggtacag
     2461 aaaacaaatg atcaaacgta acgaagagta cgcagcaaaa gcaaaataat gcgctcctat
     2521 aaaatgtaag ctcaatgaaa atcaaagagc aaactaggaa gctagccgca ggttgctcaa
     2581 aacacagttt tgaggttgta gataagactg acgaagtcag tgaccatatc tacgtcaagg
     2641 cgacgctgac gaagtttgaa ttggattttc gcagagtgta aattgttgaa actaggttgt
     2701 cataccatta ggagtgacag cctagttttt tataaactat caagaaattg gagaaaataa
     2761 tggtaattga attaaaatca gaacaattaa gtgttcaatt taacagtttt ggtggggctc
     2821 tttcttctat aaaggatgct gagggactgg agtatttgtg gcaaggggat gctacttatt
     2881 ggagtggaca agctcctgtt ctctttccaa tttgtggttc attgagagag gatacagccc
     2941 tctatataga tgagaagggg caagaaagta aaggaaagat tcctcgtcat ggtctggttc
     3001 gtaagaaaga atttgaatta gttgaacaga cagacaagag tgtaactttt gcaattgaag
     3061 acgatgagaa tagctatcaa aactatcctt atcatttccg cctagaaatc acttatagcc
     3121 ttactggaaa gacagtacgg actcaataca aaatatttaa taaagaaact agcaaggtca
     3181 tgccttactt tattggtgga catccaggat ttaattgtcc tctactggat gatgaggtct
     3241 atgaagatta ctatttagag tttgagaaag aagagacttg ctctgttcca cgtcctttcc
     3301 cagaaacggg gatgctggat tttcaagata gaagtccatg gctagaggga caaaaagaaa
     3361 tagatcttag ttatgatctt ttcagcacag atgcagtgac tttggatgaa ttgcaatctc
     3421 gaacaattgc ccttcgttct cgtaaacatg ataagggggt gaaagtgcac tttgcagagt
     3481 ttccaaacct catcatctgg tcaactttga ataagggacc tttcattgcc tttgaaccat
     3541 ggtctggctt gtcaacatcc cttgaagaag gagatcattt agaagataag aagaatgttc
     3601 gtctcttaga agccaatcag gttgaagaaa tagggtttga gatagaagtt ttataaaaaa
     3661 gtaaaaaata aataacaaaa aacaaacaca aactgttgac tttttcaaac aatagtagta
     3721 tattatgttt gtgaggacta attgactgtt ttttaaagac agccaataca ctgctggaat
     3781 ccagcataga aaaaataaaa aggagtatac aatatgtcta ttgttatcgg tgcagatgct
     3841 gcaggtttga gattgaaaga agttgtgaaa gatttcttag aaaaagaaaa tttccacctt
     3901 gtggatgtta cagctgaagg tcgagacttt gttgatgtga ctctcgctgt tgctgcagaa
     3961 gtaaacaaag aagaacaaaa ccttggtatc gtgattgatg cttatggagc tggtccattt
     4021 atggttgcaa caaaaatcaa aggaatggtt gctgcagaag tatctgacga acgttcagct
     4081 tatatgactc gtggccacaa caactctcgt atgatcacta tgggtgcaca acttgttggt
     4141 gatgaattgg ctaaaaacat cgctaaagga tttgttaatg gtaaatacga cggtggtcgt
     4201 caccaaatcc gggttgacat gttgaacaaa atgggctaat tagattatag taagaaaggt
     4261 aagttaaaaa tgagaattgc aattggatgt gaccacatcg taactgatga aaaaatggcg
     4321 gtttcagaat ttttgaaatc aaaaggatat gaagtcattg actttggtac ctatgaccat
     4381 acacggactc actacccaat ctttggtaaa aaagtagggg aagcagtaac tagtggtcaa
     4441 gctgatctcg gggtatgtat ctgtggtact ggtgttggta tcaacaacgc tgtaaataaa
     4501 gttccaggtg ttcgttctgc cttggttcgt gatatgacaa cagcccttta tgctaaagaa
     4561 caattgaacg ctaacgttat tggttttggt ggtaaaatta ctggtgaatt gcttatgtgt
     4621 gatatcatcg aagctttcat ccatgctgaa tacaaaccaa ctgaagaaaa caaaaaattg
     4681 attgcgaaaa ttgaacatgt tgaaagtcac aatgctcaac aaacagacgc aaacttcttt
     4741 acagaattcc ttgagaaatg ggatcgtgga gaataccacg actaagaggt gacctatgat
     4801 tttaacagtc acaatgaacc catccatcga tatttcctat cccttggatg agttgaagat
     4861 tgatactatc aatcgtgtgg tggatgtaac caaaacggct ggtggtaagg gactcaatgt
     4921 tacccgagta ctttcagaat ttggcgattc tgttcttgct actggtttag tgggtggcaa
     4981 acttggtgag tttttggttg aacatatcga taatcaagta aagaaagatt tcttctcaat
     5041 taagggagaa actcgtaact gtatcgctat tctccacgga gacaaccaaa cagaagttct
     5101 tgaaaaaggt cctgaagtat tggaacagga aggtcaagag tttttggaac atttcaaaaa
     5161 actcttggag tcagttgaag tagtagccat ctcaggtagt ctgccagctg gccttccagt
     5221 tgattactat gcgagcttgg tagaacttgc taatcaagct ggcaagcctg tagtcttgga
     5281 ctgctcaggt gcagcacttc aggctgttct tgaatcaccc cataaaccaa cagtcatcaa
     5341 accaaataat gaagaattgt ctcagcttct tggaagagaa gtttctgagg atttggatga
     5401 attaaaagaa gtacttcaag aacctttgtt tgcagggatt gaatggatta tcgtttcact
     5461 tggtgccaac ggtacttttg ccaaacatgg tgacactttc tacaaggtag atattcctag
     5521 aattcaggtg gtaaatcctg ttggatctgg agactctact gtggcaggaa tttcttcagg
     5581 acttcttcac aaagaatcgg atgcagaatt actcatcaag gcaaatgtcc ttggtatgct
     5641 caatgctcaa gaaaaaatga ctggtcatgt caacatggcc aactatcaag ctctatatga
     5701 tcaattaata gtaaaagagg tataaaatgg ctttaacaga acaaaaacat gtacgcttag
     5761 aaaaactttc tgatgaaaat ggtatcatct cagctcttgc atttgaccaa cgtggtgctt
     5821 tgaaacgcct catggctcaa caccaaacag aagaaccaac tgtggcccaa atggaagaac
     5881 ttaaagtctt ggtagcagat gaattgacta aatatgcttc atctatgctt cttgaccctg
     5941 agtatggact tccagcaact aaagctcttg atgaaaaagc tggtcttctc cttgcttatg
     6001 aaaaaacagg ttatgacaca acaagcacaa aacgcttgcc agactgcttg gatgtttggt
     6061 ctgcaaaacg tattaaagaa gaaggtgcag atgcagttaa attcttgctt tactatgatg
     6121 tagatagctc agacgaactc aatcaagaaa aacaagccta catcgaacgc atcggttctg
     6181 agtgtgtggc tgaagatatc ccattcttcc ttgaaatcct tgcttacgat gaaaaaattg
     6241 cggatgcagg ttctgtagaa tacgctaaag taaaaccaca caaagttatc ggcgctatga
     6301 aagtcttttc agacccacgc tttaacattg atgttttgaa agttgaagtt cctgttaaca
     6361 ttaaatatgt tgaaggcttc gctgaaggtg aagtagttta tacacgtgaa gaagcagcag
     6421 ccttcttcaa agcgcaagat gaagcaacga acttgccata catctacttg agtgctggtg
     6481 tatcagctaa actcttccaa gatactcttg tatttgctca tgaatcaggt gcaaacttta
     6541 acggagttct ttgtggccgt gctacatggg caggatcagt tgaagcttac atcaaagatg
     6601 gtgaagcagc agctcgcgaa tggcttcgca caactggatt tgaaaacatt gacgaactca
     6661 acaaagttct tcaaagaaca gcaacttcat ggaaagaacg cgtgtaagaa agtcctccta
     6721 gtttaggaac atgaatctaa aaaaatttaa aaaaagttgt atgtaaaggt ttacaaaata
     6781 acttacttgt gctatactta aatcacaagt taatatgaat tagaaagtaa ctatatgaag
     6841 tataataaaa ataggatata gtttatttta cgagctagga aggaaaaaat acggaaacaa
     6901 tattgccaga ataaactata tttagatgca catttcactc attgttttat aaaaggagaa
     6961 gataaatggc tactaaaaag agttttaaag cgttagttgt aggactaggt attgtttcaa
     7021 tattctcatc agccttacct atggttagtg gttctgtatt tgcagatagt gccctaacta
     7081 cagtagataa agcaaatgat attgttttga atgttgatgg gaataaattt tataatgttt
     7141 cggtttcaga agatattgta aatgctggtc aaattttgga agattatttt tatgtagata
     7201 aatttggaaa tataaattta aaaggcactc ctgaagagtt atcaaaaaat attggtattt
     7261 ctgtacaaga agcaagtttg atgtatgaag ctgtaaaaga gttgcccaac gtttacgaaa
     7321 gaggtcctgt aggttttcgt ttcaatcttg gtcctcaagt gagggggatg ggtggctggg
     7381 ctgctggagc tttcgctact ggatatgctg gatggcattt gaaacaattt gcggttaatc
     7441 ctgttacatc tggatttgtt gctgtaataa gtggtgcgat tggctgggct gtaaaaactg
     7501 ctgtagaaaa ttattggaca gttgctgtag ctacagtaga agtgccgttt gtgaaccttg
     7561 tttacaccat agatttacct tagaggttat ttctttatga atcattcttt taaaaaaata
     7621 actgtatttt gttttatagt ttcttgtgtt ctttgtttat tagacttaat gaattttaga
     7681 acatagttaa gttttaaaaa aagttgtatg taaaggttta caaaacaacc gacttgtgct
     7741 atacttaaat cacaagttaa tacaaggtga gtgttactaa gtaatattag gcatgatcac
     7801 aggtgaatta gaaatcagct gattttctag ttcatttgtg gtcatttttt gtactcatat
     7861 acctttaaga tataaaagga ggttgacatg tatcgaattc taaatccaat gaatcacaat
     7921 gtctcgcttg tcagaaatga taagggagaa gaggtgatta taattggtaa ggggattgca
     7981 ttcggaaaaa ggaaggggga tttgattgct gaaaatcagg ttgagaaaat ctttcggatg
     8041 aagaccgaag agtccagaga aaactttatg gctcttctca aagatgttcc gcttgatttt
     8101 atcacagtga cctatgaaat cattgataag ctatcaaaga aatatcatta tccgattcaa
     8161 gagtatctct atgtaacctt gacagatcat atttactgtt cttatcaagc tctaactcaa
     8221 ggaaggtaca aggatagtaa tctgccggat atttccgcta agtatcctgt cgcttttcaa
     8281 atcgcaaatg aagcttttga aatttaccgt cagaagctag cagatcattt tcctgaggac
     8341 gagattattc ggattgctta tcatttcatc aatgccgaag gtgaaaatga agtggaactt
     8401 gtggagtcga ttgataagag gaaagaaatt ctcaggaatg ttgaagaagt gctaaagggt
     8461 tatgcaattc aacgaaccaa aaaaaataat catttctatg atcgctttat gattcatttg
     8521 aattatttct tggattattt agacagatct agagatgata accaatcact tctggatatg
     8581 gaagatcata ttaaacaatc ctatccaaaa gccttcgaga ttggttccaa gatctatgat
     8641 gtgattacgc aacatacggg tcttgatttg tataaaagtg aacgagttta tctagttcta
     8701 catatccaac gtttattgtc ataaaaattt atttaaaact atataaggag aattctatca
     8761 tgaatagaga agaagtaaca ttgttaggtt ttgaaatcgt agcctatgct ggcgatgctc
     8821 gttcaaaact attggaagcc ttgaaggctg ctgaagctgg tgattttgaa aaagcggacg
     8881 ctctggtaga ggaagctggt agctgtattg cagaggctca ccacgcgcaa acaagtctat
     8941 tgactaagga agcttctggt gaggacttag cgtatagtgt gaccatgatg catggccaag
     9001 accacttgat gacaactatc ttgttaaagg atttgatgca ccatttgatc gaactctata
     9061 agagaggagt taaataatga acaaactaat tgcatttatc gagaaaggaa agcctttctt
     9121 tgagaaacta tctcgtaata tctatcttcg tgctattcgt gatggtttca ttgcaggtat
     9181 gcctgttatt ctcttctcaa gtatctttat cttgattgcc tttgtaccaa actcatgggg
     9241 ctttaaatgg tctgatgaag ttgtagcctt tctgatgaaa ccttatagct attctatggg
     9301 tattctggct ctcttggtag ctggtacaac agctaagtca ttgaccgact cagtaaaccg
     9361 tagcatggag aaaaccaatc aaatcaacta tatgtcaacg ctgttagcag cgattgttgg
     9421 tttgttgatg ttggcagctg atcctatcga aaatggtcta gctactggat tcttggggac
     9481 aaaaggtttg ctttcagcct tccttgctgc ctttgttact gtagccatct ataaggtctg
     9541 tgttaagaac aacgtcacta ttcgtatgcc tgacgaagtt ccaccaaata tctcacaagt
     9601 ctttaaagat gtgattccat tcactctatc agtggtttct ctttatgctc ttgatttact
     9661 agctcgtcat tttgttggtt ctagtgtggc agaatcaatc ggtaaattct tcgcaccact
     9721 cttctcagca gcagacggat accttggtat taccattatc tttggtgcct ttgccttctt
     9781 ctggtttgtg ggtattcatg gtccatctat tgttgaacct gctatcgcag cgattaccta
     9841 tgcaaatgct gaagtcaact tgaaccttct ccaacaaggt atgcatgctg ataaaatcct
     9901 tacttcagga actcaaatgt ttatcgttac tatgggaggt actggtgcga cactggttgt
     9961 tccattcatg ttcatgtggt taacaaaatc aaaacgtaac cgtgcaatcg gacgtgcttc
    10021 agtagttcca actttctttg gtgtaaatga accaatcttg tttggtgcac cacttgtatt
    10081 aaatccaatc ttcttcattc catttatctt tgctccaatc gcaaacgtat ggattttcaa
    10141 attctttatt gaaactcttg gaatgaactc attcactgct aatctaccat ggacaactcc
    10201 agctccacta ggtctagttc ttggaactaa cttccaagtg ctatcattca ttcttgctgc
    10261 ccttctaatc gtggttgacg ttgtcattta ctatccattc cttaaggtct atgatgaaca
    10321 aattcttgaa gaagaacgtt caggtaagtc taatgatgaa ttgaaagaaa aagttgctgc
    10381 aaacttcaac actgcaaaag cggatgctat tcttgaaaaa gcgggtgtag aagcagcaca
    10441 aaatacaatc actaaagaaa caaatgtcct cgttctctgt gcaggtggag gaacaagtgg
    10501 tctccttgca aatgctttga ataaggcagc agcagaatac aatgtccctg tgaaagcagc
    10561 agcaggcggc tatggtgctc accgtgaaat gttaccagag tttgatcttg ttgtccttgc
    10621 ccctcaagtt gcttcaaact ttgaagatat gaaagcagaa acagataagc tcggtattaa
    10681 actagcgaaa acagaaggcg ctcaatacat caaattaact cgtgatggaa aaggtgccct
    10741 agcattcgta caagcgcaat tcgattaagg ctagagactc tgaaattgtc tcctatcgtt
    10801 acggaaatcg ctatggcgaa tttcctaatc gccttattaa ttcggtgact tggtacaaga
    10861 agtgagatgg agaaggatgg ctcactgact cccctcctct cacttttact ttatttaaat
    10921 caagaaatag gtgaaaaaaa tgacaaaaac acttccaaaa gactttattt ttggtggcgc
    10981 aacagctgcc tatcaagcag aaggtgctac acatactgat ggtaaaggac cagtagcgtg
    11041 ggataaatat cttgaagata actactggta cactgcagaa ccagccagtg atttttacaa
    11101 tcgatatcca gttgacctca agctagcaga agagtatggt gtcaatggca ttcgaatttc
    11161 tattgcctgg tcacgtattt tcccgactgg ttatggtcaa gtaaatgcta aaggtgttga
    11221 gttttatcat aatctatttg cggagtgcca caaacgtcat gttgagccct ttgtaactct
    11281 tcatcacttt gacactccag aagctctcca ctcaaatgga gacttcttaa accgtgaaaa
    11341 cattgaacac tttgtagact acgcggcttt ctgttttgaa gaatttccag aagtaaacta
    11401 ttggacaacc ttcaatgaaa ttggaccaat cggtgatggt caatatttag ttggtaaatt
    11461 ccctccagga attcagtacg accttgccaa agtcttccaa tcgcaccaca atatgatggt
    11521 gtctcatgca cgtgctgtaa agctatataa agataaagga tataaagggg aaattggtgt
    11581 tgttcacgcc ctgcctacta aatatcctct agatcatgaa aatccagcag atgttcgtgc
    11641 agctgagttg gaagatatca tccacaataa atttattttg gatgcaacct atctgggtca
    11701 ctactctgaa aagactctgg caggggtaga ggctatctta tcagtcaatg gtggtagttt
    11761 ggatcttcgt gaagaagatt ttacagcatt agaagctgca aaagacttga atgatttcct
    11821 aggaatcaac tactatatga gtgactggat ggaagccttt gatggagaaa ctgaaattat
    11881 ccataatggt aaaggtaaaa aaggaagctc taagtatcaa atcaaaggtg ttggtcgtcg
    11941 tgtagctcct gactatgtac cacgcacgga ttgggattgg attatctacc ctcaaggttt
    12001 gtatgaccaa atcatgcgtg tgaagaaaga ttatcctaac tacaagaaga tttacatcac
    12061 tgaaaatggt ctcggctata aagatgagtt cgttgataac actgtttacg atgatggtcg
    12121 tattgattac gtgaaacaac acttggagat cttgtctgat gcgattgcag atggagctaa
    12181 tgtaaaaggt tacttcattt ggtcattaat ggatgtcttc tcatggtcaa acggttatga
    12241 gaaacgttat ggtctcttct atgtagactt tgaaactcaa gaacgttatc ctaagaaatc
    12301 agctcactgg tacaagaaag tagcggaaac tcaagtaatt gagtagtaga attagtaatt
    12361 agatatagaa ttttagtgag gcaaaaagat gttcaaagat tttatccaat ctatttatga
    12421 aaaagtttat attatcaatt ttgataaatg ttctcaaata ccgtgtttga cgaatgaaga
    12481 actgaaaagt ctcggaaaat ggtatgtcta taccggtaaa gaatggattt gccattcaga
    12541 ctatgagctg gaagaattta aaaatatctt tttaaatttt atcaatcctg aagaatggga
    12601 tactatctcc tttgattcag attttatgcc gttttaacaa tcgtaaccaa tttccaaaaa
    12661 agttaaatct cgtatttagt acgctgcaaa actcttatct aatcacgttg cttatactca
    12721 atgaaaatca aagagcaact ttaaactaga aagcgagtcg caggtttctc aatgcatcgc
    12781 tttgaggttg cagataaagc aattaggcaa aaagtcttta acgcagaaaa acgcataata
    12841 tcaggtttaa aaaaccttga tactatgcgt tttattgtgg gaagatttac ttctgaaatt
    12901 gagttgttac ccagaatctt tcagtttatt aaggcttgat gactttagta agtttagata
    12961 gttcaaaaag gattgaatca cttagtttag aatctgaaac aatagtatca agatttgata
    13021 cattataaaa agtataaaaa tcaaacttat tgaacttact atgatctgcg agtaaatatt
    13081 ttttattaga attatttaaa gcgatgcgtt gagcctctcc ctcttcctcg ctaaaagtag
    13141 ctagagctcc gttttgaata ccattacagc taacgaaagc tttagaaaat tggagattag
    13201 agagattttg tagggtcaat gtgccaacaa aagcacctgt aatatcgcga taatttccac
    13261 ctatcaaaat caaatctgtt aattttcgtt cgcttaaaat cagaaaaaca ggtagactgt
    13321 tggtcacgac gcggatattg tcaataggca actcacgcgc aaaaaattct aatgttgttc
    13381 ctggtccaat gaaaatagtt tctctttctt ctactagact gcctgcaaaa cgggctattt
    13441 cttgtttttc tgccgtttgg agggcttgtt tttcaatatt tgatcgctca ttagtcaaaa
    13501 gggagttggt tcgaagtttt tcagctccac catgcacacg aatcagcaaa tctttatcag
    13561 ctaattcctg taaatagcgc cttgcagtca tatctgaaac ggctatttcg tccataatct
    13621 gtttaaccgt tatggttcct ttactattta ctatctctaa aattttggtt aatttttctt
    13681 gtttgagcat attatcacct cgtttcctac tactatctta ccataaacta gctcatcatt
    13741 caaatacaaa aacaacaaaa taaaacaaaa acaaaaatat cgaggtttat tttcaaaact
    13801 ttcgatattt ttattaagtt attattttgt tgtttctagt ttactttttg atgattgaga
    13861 gtggtggaga attatactca atgaaaatca aagagcaaac taggaagcta gccgtaggtt
    13921 gctcaaaaca cagttttgag gttctagata agactgatga agtcagtgag catatctacg
    13981 gcaaggcgaa gctgacgtgg tttgaagaga ttttcgaaga gtattagtcc aaaccgtagt
    14041 tgtagtcatc gtcttgcatg gcttcaactt cgccaagaag gtaaccattt ccgacttgag
    14101 agaagaagtc atggttggaa gttcctgttg aaataccgtt cataacgatt gggttgacat
    14161 cttcagctga atctgggaaa agtggatctt gtcccatgtt catgagagct ttattggcat
    14221 tgtagcgaag gaaggtttta acctcttcag tccaaccaac accgtcataa agactctctg
    14281 tgtagccttc ttcattttca taaagagtat agagtaggtc gtacatccat tctttgagtt
    14341 tttcttgctc ttcttcaggc aattcattga aaccaagttg gaatttgtaa ccaatgtagg
    14401 ttccgtgaac agactcgtca cgaataatca atttaatgat ttctgcaacg ttggctagtt
    14461 tgttgttacc gagatagtag aggggagtga agaaaccaga gtagaagagg aaggtttcga
    14521 ggaagacgct ggcaactttc ttttcaagtg ggctgccgtt taggtagatt tcgttgacaa
    14581 tctcagcctt cttttgtagg taaggattgg tattggtcca ttcgaaaatt tcttcaatct
    14641 cagccttagt attcaaggta gaaaagattg atgagtaaga tttagcgtgg acagattcca
    14701 taaattggat gttattgaaa acagcttcct catgtggtgt acggatgtct gcgcgaaggg
    14761 cttgaacccc agtttcagat tgcatagtgt caagaagggt taaaccacca aaaacttttc
    14821 cgaccaagtc tttctctttg ttagatagct ttctccagtc atccaagtcg tttgataagg
    14881 gaatacgtgt atcgagccaa aattgctccg tcagtttttc ccaagttgat ttgtcgatga
    14941 catcttcgat ggcattccag ttaatggctt tgtagtaagt ttccatttaa aatctctttc
    15001 tgtgtttagt attgcgaact cacaattatt tctactttac cataattcta taggagtatc
    15061 gcacaaaaag tcggaagccc gacttttaaa atgttacata aattatgtta tgacatagta
    15121 gatttgattt tatcagtgct gcttagggaa aaataatgtt tctatgctag aaactaaatc
    15181 acacagcttt cacattggtt ggcgccgact tctccaccgt catctgtaaa ggtacggacg
    15241 tagtagatag acttgattcc cttgttaaag gcatagttac gaaggatgga caagtcacgt
    15301 gtcgtttgtt tattttccct cttccattcg taaaggcctt ttggaatgtc actacgcatg
    15361 aagagggtga gtgaaagtcc ttgatccacg tgttcagtcg cagcagcgta aacatcgatg
    15421 actttacgca tatccatatc gtaggcagaa gtgtagtaag gaatggtttc tgtagacaag
    15481 ccagcagcag ggtagtagat tttaccaatt ttcttctctt ggcgttcttc gatacgttgc
    15541 gtaatcgggt ggatagaagc agaaacgtcg ttgatatagc tgatagaacc atttggcgct
    15601 acagcaaggc gattttggtg gtaaagacca tcttcttgaa ccttgtcgcg aagttcagcc
    15661 caatcagcaa caccagggat aaagacattt ttgaagagtt ctttaacacg gtctgatgtt
    15721 ggaacaaatt caccagttac atacttgtca aagtaacttc cgttagcata gtctgatttt
    15781 tcaaagttgt ggaaggtaat accacgttca cgcgcaatat tgtttgactc taccaaggtc
    15841 cagtagttca taagcataaa gtagatgctt gtaaattcaa cagactcagg tgaaccatat
    15901 tcaatgagtt gttgggcaag gtagctgtgc agtcccatgg caccgagacc aaaggtgtgg
    15961 gcttggctat ttccatggtc aatcgttggt acagctacga tatgtgaact atctgtaacg
    16021 aaagtaaggg cacgaaccat agcacggata gaacgaccaa aatcaggtga agtcatcatg
    16081 ttaaccacgt tggttgaacc caggttacat gaaacatctg ttcccatttg aaggaattct
    16141 tgagcatcgt tgatcaagct tggttcttga acttgaagaa tctcagaaca caagttactc
    16201 atgataatct ttccatcaac aggatttgca cggttagccg tatcgatgtt gactacataa
    16261 ggatagccag actcttgttg caatttagag atttcagttt ccaaatcccg cgccttgatt
    16321 tttgtcttgc gaatatttgg atttgcgacc aattcatcgt atttttcagt aatgtcgatg
    16381 taattgaatg gcacaccgta ttctttttct acagagtaag ggctgaagag gtacatttct
    16441 tcatttttac gagccaattc gtagaattta tcaggtacta caacaccaag tgatagagtc
    16501 ttgacacgta ctttttcatc agcgttttct ttcttagttg aaaggaaagc gatgatatct
    16561 gggtgaaaga cgttgaggta gacaacaccg gcaccttgac gttgccccaa ttggttggag
    16621 taagagaagc tgtcttcaaa aagcttcata acaggaacga cacctgaagc agctccttca
    16681 tagcctttga taggtgcacc agcttcacga aggttgctga gggtaattcc cacaccacca
    16741 ccaatacgtg aaagttgaag agctgagttg atagaacgtc cgatagagtt catatcatcc
    16801 gtcacttgga ttaggaaaca agataccaac tccccacgac gagcacgtcc agcattcaag
    16861 aaggaaggag tagcaggttg gtagcgttgg tggatgattt cattggcaat atcgattgca
    16921 acagcttcat tcccatcagc gaaataaagg gcgttgaaga agacacggtc ttccatattt
    16981 tcaagatagt attcaccgtc attagtcttt aaggcatatt gattgtaaaa tttataagct
    17041 gccatgaatg acttgaattg gaagttttgg tctttgataa attgagccaa ttcttccaag
    17101 aactctggac ggtatttctt gataaaggct gtttcgatgt agttgtgttc aatgaggtaa
    17161 ttgattttgt ctttgattga atcaaaaacc atagtgtttg gaactacatt ttctttaaag
    17221 aaagcatcca aggcttcctt atctttatga agcatgattt gtccattaac aggacggtta
    17281 atttcgttat taagacggaa gtaagtcacg tcttcaagat gttttaatcc cataaaattt
    17341 cccttatcta attacaaaag aaaggcttct aagttagccc taaaagcagt ttcttctgga
    17401 tgatgtacta agattatgct aattgtttca gttttcctgg ttggaaacct gaaaagactt
    17461 cagttggtgt ttggataaca ggagctgcgc taaaaccgag ctctttaact tgatcgacgt
    17521 actcaggttg ctcatcaaga ttgatttcac gataagagac attattactg tccaagaaac
    17581 gcttggtcat tttacattgg acacaattgt ttttagaata aacggttacc attgtgtaac
    17641 tcctcttcaa aatttaatac tatcttagta tatcagaaaa taaaattttg tcaatgattt
    17701 aaactaaata tagtggtgtt cttttgttgt tttcatatat aaacacaata tatagtgttt
    17761 gtattgtgaa at
//
LOCUS       DAVKDZ010000039        17494 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000039 DAVKDZ010000000
VERSION     DAVKDZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17494
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.040"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            591..1043
                     /locus_tag="VWZ30_001311"
     CDS             591..1043
                     /locus_tag="VWZ30_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9335035.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1062..2399
                     /locus_tag="VWZ30_001312"
     CDS             1062..2399
                     /locus_tag="VWZ30_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU9335036.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     ESLYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAGKEAEKQLNDLFEAVQ"
     gene            2478..3362
                     /locus_tag="VWZ30_001313"
     CDS             2478..3362
                     /locus_tag="VWZ30_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9335037.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTIFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3362..4195
                     /locus_tag="VWZ30_001314"
     CDS             3362..4195
                     /locus_tag="VWZ30_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9335038.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4213..6306
                     /gene="nanB"
                     /locus_tag="VWZ30_001315"
     CDS             4213..6306
                     /gene="nanB"
                     /locus_tag="VWZ30_001315"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEU9335039.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHSNELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6318..7421
                     /locus_tag="VWZ30_001316"
     CDS             6318..7421
                     /locus_tag="VWZ30_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9335040.1"
                     /translation="MVKYGVVGTGYFGAELARYMQKNDGAEITLLYDPDNTEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHRAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7594..8292
                     /locus_tag="VWZ30_001317"
     CDS             7594..8292
                     /locus_tag="VWZ30_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9335041.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPHEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVKAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8311..9828
                     /locus_tag="VWZ30_001318"
     CDS             8311..9828
                     /locus_tag="VWZ30_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9335042.1"
                     /translation="MFKVLQKVGKAFMLPIAILSAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYSNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHMIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADILNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADHLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9952..11280
                     /locus_tag="VWZ30_001319"
     CDS             9952..11280
                     /locus_tag="VWZ30_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9335043.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGNKDAGKSGGDGAKTEIT
                     WWAFPVFTQEKAGDGVGTYEKSIIEAFEKANPDVKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIIQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11531..12397
                     /locus_tag="VWZ30_001320"
     CDS             11531..12397
                     /locus_tag="VWZ30_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9335044.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSITKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12418..13251
                     /locus_tag="VWZ30_001321"
     CDS             12418..13251
                     /locus_tag="VWZ30_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003036967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9335045.1"
                     /translation="MYRKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPDTI
                     VIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAKKR
                     FYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLMKQ
                     FSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLVML
                     TSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGAVK
                     G"
     gene            13311..13655
                     /locus_tag="VWZ30_001322"
     CDS             13311..13655
                     /locus_tag="VWZ30_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335046.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMAWIINGIVNGLIGSYIKLGTY
                     AAKYGISMARSILSRAAATAAARVGLLTKIFGWILRIAVLGADVYGNFANNIAVAWDA
                     HDKIPNNGRINF"
     gene            13942..14586
                     /locus_tag="VWZ30_001323"
     CDS             13942..14586
                     /locus_tag="VWZ30_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9335047.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEIKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLILASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            14755..15672
                     /locus_tag="VWZ30_001324"
     CDS             14755..15672
                     /locus_tag="VWZ30_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU9335048.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTATHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            15690..16574
                     /locus_tag="VWZ30_001325"
     CDS             15690..16574
                     /locus_tag="VWZ30_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9335049.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFQEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(16590..17441)
                     /locus_tag="VWZ30_001326"
     CDS             complement(16590..17441)
                     /locus_tag="VWZ30_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU9335050.1"
                     /translation="MNKPDIATVIDSHFEEMTDLEQEIARYFLQAETIKDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
BASE COUNT         5489 a         2951 c         3690 g         5364 t
ORIGIN      
        1 aaaaaaaaag tatactctaa ttgaaaacgg taacaaaact aatttagaga atgaaatata
       61 gagtatttct ctcttaaaag tttttggtga aacgagatgt agaaaggaga tttagccaaa
      121 gagtctatta gtgctagaat aatagattag aattatttta gaaaaacgaa gtgagcagct
      181 tataaattca agtccccaaa tagattcata ctagtatctt ttgcaaaaaa taaagggcga
      241 cttccttcat gaatatcaat ttcatctata aggaaggtag ctaattgaac taacttattt
      301 attctgtttg tcgctagaaa aatcagacct ccttgtgaag attgaggaga tacttaatga
      361 aaatcaaaga agaaactagc aagctagtag cagattgccc aaaacaccgc tttgaggttg
      421 tagataagac tgacgtatat aatccaaggt gaagcgactg tggtttgaag agattttcaa
      481 agagtatagg ctagagagta gtgtttttat gtccttctag tagaaaatgc tagacagaag
      541 aatggggaac ttggatagga aaaatagatt gagaaaggag gttagaagag atgattatta
      601 caaaaattag ccgtttagga acttatgtgg gagtaaatcc acattttgca acattaatag
      661 attttctaga aaaaacagga ctagaaaatt taacagaagg ttcgattgct atcgatggta
      721 atcgattgtt tgggaattgc tttacttatc tagcagatgg tcaagcaggg gctttctttg
      781 aaacacacca aaaatatttg gatattcatt tagttttgga aaacgaagaa gccatggctg
      841 ttacatcgcc ggaaaatgta agcgttaccc aagagtatga tgaagagaaa gatattgaat
      901 tatacacagg gaaagtggaa cagttggttc atttgagagc tggcgaatgc ctcatcactt
      961 ttccagaaga tttacatcaa cccaaggttc gtataaatga tgaacctgtg aaaaaagttg
     1021 tctttaaagt tgcgatttct taatgtagaa agagaagaac gatgaaaaaa atgagaaagt
     1081 ttttatgtct agctggaatt gcgctagcgg ctgttgcctt ggtagcttgt tcaggaaaaa
     1141 aagaagctac aactagtact gaaccaccaa cagaattatc tggtgagatt acaatgtggc
     1201 actcctttac tcaaggaccc cgtttagaaa gtattcaaaa atcagcagat gctttcatgc
     1261 aaaagcatcc aaaaacgaaa atcaagattg aaacattttc ttggaatgac ttctatacta
     1321 aatggactac aggtttagca aatggaaatg tgccagatat cagtacagct cttcctaacc
     1381 aagtaatgga aatggtcaac tcagatgctt tggttccgct aaatgattct atcaagcgta
     1441 ttggacaaga taaatttaac gaaactgcct taaatgaagc aaaaatcgga gatgattact
     1501 actctgttcc tctttattca catgcacaag tcatgtgggt tagaacagat ttgttaaaag
     1561 aacataatat tgaggttcct aaaacttggg atcaactcta tgaagcttct aaaaaattga
     1621 aagaagctgg agtttatggc ttgtctgttc cgtttggaac aaatgactta atggcaacac
     1681 gtttcttgaa cttctacgta cgtagtggcg gaggaagcct cttaacaaaa gatcttaaag
     1741 cagacttgac aagccaactt gctcaagatg gtattaaata ctgggttaaa ttgtataaag
     1801 aaatctcacc tcaagattct ttgaacttta atgtccttca acaagctacc ttgttctatc
     1861 aaggaaaaac agcatttgac tttaactctg gcttccatat cggaggaatt aatgccaata
     1921 gtcctcaatt gattgattcg attgatgctt atcctattcc aaaaatcaaa gagtctgata
     1981 aagaccaagg aattgaaacc tcaaacattc caatggttgt ttggaaaaat tcaaaacatc
     2041 cagaagttgc taaagcattc ttagaatcac tttataatga agaagactac gttaaattcc
     2101 ttgattcaac tccagtaggt atgttgccaa ctattaaggg gattagcgat tctgcagcct
     2161 ataaagaaaa tgaaactcgt aagaaattta aacatgctga agaagtgatt actgaagctg
     2221 ttaaaaaagg tactgctatt ggttatgaaa atggaccaag tgtacaagct ggtatgttga
     2281 ctaaccaaca cattattgaa caaatgttcc aagatatcat tacaaatgga acagatccta
     2341 tgaaagctgg aaaagaagca gaaaaacaat taaacgattt atttgaggct gttcagtaga
     2401 tgtaaaagac tagaaaatag gtgggctagt gagctgaaaa gctctagccc aatcttgtaa
     2461 aagaagggag aaggagaatg gttaaagaac gtaatttaac tcgctggata tttgttttgc
     2521 cagctatgat tatcgtagga ttactctttg tttatccgtt tttctcgagt attttttata
     2581 gctttaccaa taagcatttg attatgccca attataaatt tgttggttta gctaactata
     2641 aagctgtgct atcagatccc aacttcttta atgcgttctt taattcaatt aagtggacca
     2701 ttttctcatt agttggtcaa gttttagtag ggtttgtatt ggctttagct cttcatagag
     2761 tacgccactt taagaaatta tataggacct tattgattgt tccttgggca tttcctacaa
     2821 tcgttattgc cttctcttgg caatggattc taaacggggt ttatggctat ttacctaatc
     2881 taatcgtaaa attaggttta atggaacata cacctgcatt tttgacagat agtacatggg
     2941 cattcctatg tttggtgttt atcaacattt ggtttggagc gccgatgatt atggttaatg
     3001 tgctttcagc tttgcaaaca gtaccagaag aacaatttga ggctgctaag atagatggtg
     3061 cttcaagttg gcaggtgttc aagtttatcg tctttccaca tattaaagta gttgtagggc
     3121 ttctagttgt tttgagaact gtatggatct ttaataactt tgacattatc taccttatta
     3181 cgggtggtgg accggctaat gctacaacga cgcttccaat ttttgcttac aacctgggtt
     3241 ggggaactaa attgttgggt cgtgcttcag cagttacagt attgctcttt atcttcttgg
     3301 tggcgatttg ctttatctac tttgctatca tcagtaagtg ggaaaaggag ggtagaaaat
     3361 aatgaagaag aaatccagta tttatttaga tattctctca catgtacttt tagttggtgc
     3421 gaccatcgtt gcaattttcc cattggtatg gattatcata tcttctgtca aagggaaagg
     3481 ggaattaact cagtatccaa cacgattttg gcctgaacag tttacattag attatttcac
     3541 tcatgttatc aacgatttgc acttcattga taatattcga aacagtttaa tcattgcctt
     3601 ggctacaacc ctcattgcga ttattatttc tgctatggca gcctatggta ttgttcgatt
     3661 ctttcctaaa ttgggagcaa tcatgtcgag actactcgtc attacctaca ttttcccacc
     3721 aattttgtta gcaattccct attcaattgc cattgctaaa gttgggttaa caaatagttt
     3781 atttggcttg atgatggttt atctatcttt tagtgttcca tatgcagttt ggctcttagt
     3841 tggatttttc caaacagttc caattggaat tgaagaagcg gctagaattg atggtgcaaa
     3901 taaatttgtt acgttttata aagttgtgct accgattgta gcaccaggta ttgtagcaac
     3961 agctatttat acatttatca atgcttggaa tgaattcctg tatgccttga ttttgattaa
     4021 caatacagga aagatgacag tagcagtagc ccttcgttca cttaatggtt cagaaatact
     4081 agactgggga gatatgatgg cagcgtctgt tattgtagtt cttccatcaa ttattttctt
     4141 ctctatcatc caaaataaga ttgcaagtgg attatcagaa ggatctgtga agtagacgaa
     4201 agaaggaaaa aaatgaataa aagaggtctt tattcaaaac taggaatttc tgttgtaggc
     4261 attagtcttt taatgggagt ccccactttg attcattcga atgaattaaa ctatggtcaa
     4321 ctgtccatat ctcctatttt tcaaggaggt tcatatcaac tgaacaataa gagtatagat
     4381 atcagccctt tgttattaga taaattgtct ggagagagtc agacagtagt aatgaaattt
     4441 aaagcagatg aaccaaactc tcttcaagct ttgtttggcc tatctaatag taaagcaggc
     4501 tttaaaaata attacttttc aattttcatg agagattctg gtgagatagg tgtagaaata
     4561 agagacgccc aaaagggaat aaattattta ttttccagac cagcttcatt atggggaaaa
     4621 cataaaggac aggcagttga aaatacacta gtatttgtat ctgattctaa agataaaaca
     4681 tacacaatgt atgttaatgg aatagaagtg ttctctgaaa cagttgatac atttttgcca
     4741 atttcaaata taaatggtat agataaggca acactaggag ctgttaatcg tgaaggtaag
     4801 gaacattacc ttgcaaaagg aagtattgat gaaatcagtc tatttaacaa agcaattagt
     4861 gatcaggaag tttcaaatat tcccttgtca aatccatttc agttaatttt ccaatcagga
     4921 gattctactc aagctaacta ttttagaata ccgacactat atacattaag tagtggaaga
     4981 gttctatcaa gtattgatgc acgttatggt gggactcatg attctaaaag taagattaat
     5041 attgccactt cttatagtga tgataacggg aaaacgtgga gtgagccaat ttttgctatg
     5101 aagtttaatg actatgagga gcagttagtt tactggccac gagataataa attaaagaat
     5161 agtcaaatta gtggaagtgc ttcattcata gattcatcca ttgttgaaga taaaaaatct
     5221 gggaaaacga tattactagc tgatgttatg cctgcgggta ttggaaataa taatgcaaat
     5281 aaagccgact caggttttaa agaaataaat ggtcattatt atttaaaact aaagaagaat
     5341 ggagataacg atttccgtta tacagttaga gaaaatggtg tcgtttatga tgaaacaact
     5401 aataaaccta caaattatac tataaatgat aagtatgaag ttttggaggg aggaaagtct
     5461 ttaacagtcg aacaatattc ggttgatttt gatagtggct ctttaagaga aaggcataat
     5521 ggaaaacagg ttcctatgaa tgttttctac aaagattcgt tatttaaagt gactcctact
     5581 aattatatag caatgacaac tagtcagaat agaggagaga gttgggaaca atttaagttg
     5641 ttgcctccgt tcttaggaga aaaacataat ggaacttact tatgtcccgg acaaggttta
     5701 gcattaaaat caagtaacag attgattttt gcaacatata ctagtggaga actaacctat
     5761 ctcatttctg atgatagtgg tcaaacatgg aagaaatcct cagcttcaat tccgtttaaa
     5821 aatgcaacag cagaagcaca aatggttgaa ctgagagatg gtgtgattag aacattcttt
     5881 agaaccacta caggtaagat agcttatatg actagtagag attctggaga aacatggtcg
     5941 gaagtttcgt atattgatgg aattcaacaa acttcatatg gcacacaagt atctgcaatt
     6001 aaatattctc aattaattga tggaaaagaa gcagtcattt tgagtacacc aaattctaga
     6061 agtggccgta agggaggtca attagttgtc ggtttagtca ataaagaaga tgatagtatt
     6121 gattggaaat atcactatga tattgatttg ccttcgtatg gttatgccta ttctgcgatt
     6181 acagaattgc caaatcatca tataggtgta ctgtttgaaa aatatgattc gtggtcgaga
     6241 aatgaattgc atttaagcaa tgtagttcag tatatagatt tggaaattaa tgatttaaca
     6301 aaataaagga gaaaaacatg gttaaatacg gtgttgttgg aacagggtat tttggagctg
     6361 aattggctcg ctacatgcaa aagaatgatg gagcagagat tactcttctc tatgatccgg
     6421 ataatacaga ggcgattgca gaagaattgg gagcaaaagt agcaagttcc ttagatgagt
     6481 tggtttctag cgatgaagta gattgtgtca tcgtcgcaac tccaaataat cttcataagg
     6541 aaccggttat taaggctgca cagcatggta aaaatgtttt ctgtgaaaaa ccaattgcgc
     6601 tttcttatca agattgtcgc gagatggtag atgcatgtaa agaaaacaat gtaaccttta
     6661 tggcaggaca tattatgaat ttctttaatg gtgttcatcg tgcaaaagaa ctcattaatc
     6721 aaggagttat cggagacgtt ctatattgtc atacagctcg taatggttgg gaagaacaac
     6781 aaccgtcagt atcatggaaa aaaattcgtg aaaaatcagg tggtcacttg tatcaccaca
     6841 tccatgaatt ggattgcgtt caattcctta tggggggcat gcctgaaact gtaaccatga
     6901 caggtggaaa tgtggcccat gaaggtgaac atttcggtga tgaagatgat atgatttttg
     6961 tcaatatgga attttctaat aagcgttttg ccttgttaga atggggttca gcttatcgtt
     7021 ggggtgaaca ttatgtctta atccaaggaa gcaaaggtgc catccgctta gacttattca
     7081 actgtaaagg aactcttaag ctagatgggc aagaaagcta tttcttgatt cacgaatcgc
     7141 aagaagaaga tgatgatcgg actcgtatct atcatagtac agagatggat ggagcaattg
     7201 cttatggtaa accaggtaaa cgtactccat tatggctatc atctgtcatt gataaagaaa
     7261 tgcgctatct gcatgagatt atggaaggag ctccagtatc agaagaattt gcaaaacttt
     7321 tgacaggtga agctgcccta gaagcaattg ctactgcaga tgcttgtacc cagtctatgt
     7381 ttgaagatcg caaagtaaaa ttgtcagaaa ttgtaaaata aattttggta ttctcctatt
     7441 ataggtcgac ttgctcctct gaaagtactt ttagaggagc tgtttgactt ggctagtttt
     7501 tgaaactgaa atctattata ctacaaacta ttgaaagcgt tttaatttta aggtataata
     7561 atctcataga aataaagaaa aggaggaaag aggatgccac agattagcaa agaagccttg
     7621 attgagcaaa tcaaagatgg aatcatcgtt tcttgtcagg ctcttcctca tgaaccgctt
     7681 tatacagaag cgggaggggt gattcccttg ctggtcaaag cggctgagca aggtggagca
     7741 gtcggtatcc gagcaaacag tgttcgcgat atcaaggaaa ttaaggaagt cactaaactt
     7801 ccaatcattg ggattatcaa acgtgattat ccacctcatg aacccttcat cacggctact
     7861 atgaaagaag ttgatgaatt ggcagaactg gacatcgagg tgattgctct ggattgtacc
     7921 aagcgtgaac gctacgatgg tttggaaatt caagagttca ttcgtcaggt taaggagaaa
     7981 tatcctaatc agcttttgat ggctgatact agtatcttcg aagaagggct agcagctgta
     8041 aaagcaggaa ttgactttgt cggaacaacc ttatcaggct acacatccta cagtccaaaa
     8101 gtagacggtc cagattttga attgattaag aaactctgtg atgccggtgt agatgtcatt
     8161 gcagaaggaa aaattcatac accagaacaa gccaaacaaa tccttgaata tggagtgcga
     8221 ggcatcgttg ttggtggcgc tattactaga ccaaaagaga ttacagaacg cttcgttgct
     8281 agtcttaaat aagatgtgag ggggagtttt atgtttaaag ttttacaaaa agttggaaaa
     8341 gcttttatgt tacctatagc tatactttct gcagcaggtc tacttttggg gattggtggt
     8401 gcactttcaa acccaaccac gatagcaact tatccaatac tagacaatag tatttttcaa
     8461 tcaatattcc aagtaatgag ctctgcagga gaggttgtat tcagtaattt gtcactactt
     8521 ctctgtgtgg gattatgtat tggcttagcg aaacgagata aaggaaccgc tgcgttagca
     8581 ggagtaactg gttacttagt tatgactgca acgatcaaag ctctggtaaa actttttatg
     8641 gcagaaggat ctgcaattga tactggagtt attggagcat tagttgtcgg aatagttgcc
     8701 gtatatttgc acaaccgata tagcaatatt caattacctt ccgctttagg attctttgga
     8761 ggttcacgct tcgttcctat tgttacatcg ttctcttcta tcttaattgg ctttgtcttc
     8821 tttgttattt ggccaccttt ccaacaactt cttgtttcta caggtggata tatttctcag
     8881 gcaggtccaa ttggaacttt tctatatgga tttttaatga gactttctgg agcagtaggc
     8941 ttacatcata tgatttatcc tatgttttgg tatactgaac ttggtggtgt tgaaactgtt
     9001 gcaggacaaa cagtggttgg ggctcaaaaa atattttttg ctcaattagc cgatccggct
     9061 cattctggat tatttacaga aggaacaagg ttttttgcag gtcgtttctc aacaatgatg
     9121 ttcggtttac cggctgcctg tttagcgatg taccatagtg ttcctaagaa tcgtcgtaaa
     9181 aaatacgcgg gtttgttttt tggagttgct ttaacatctt ttattaccgg tattacagaa
     9241 ccaattgaat ttatgtttct attcgtcagt ccggttctat atgttgttca cgcattcctt
     9301 gatggtgtca gcttctttat tgcagacatc ttaaatatct caataggaaa cacattttca
     9361 ggaggtgtaa tcgatttcac tttatttgga attttgcagg ggaacgctaa gacgaattgg
     9421 gttcttcaga ttccatttgg acttatttgg agtgttttgt attatattat ttttagatgg
     9481 ttcattactc aattcaacgt tctaacgcca ggacgaggag aagaagtaga ttctaaagaa
     9541 atttctgaat ccgcagattc aacttcaaat actgcggatc atttaaaaca ggatagccta
     9601 caaattatca gagcgttggg tggatcaaat aatatagaag atgtagatgc ttgtgtgaca
     9661 cgtttacgtg tagctgtaaa agaagttaat caagttgata aagcactttt aaaacaaatt
     9721 ggtgcagttg atgtcttaga agtgaagggt ggcattcaag caatctatgg agcaaaagca
     9781 atcttatata aaaatagtat taatgaaatt ttaggtgtag atgattaagt acttactgac
     9841 ttaataaaaa atagaggaga gtgattgatg agtaggaaaa aatgaaaacg tatataaatg
     9901 caaagaactc attatccaag ttggatacgc ttataaaata ggagaataca aatgaaattt
     9961 agaaaattag cttgtacagt acttgcgggt gctgcggttc ttggtcttgc tgcttgtggc
    10021 aattctggcg gaaataaaga tgctggtaaa tcaggtggtg acggtgccaa aacagaaatc
    10081 acttggtggg cattcccagt atttacccaa gaaaaagctg gtgacggtgt tggaacttat
    10141 gaaaaatcaa tcatcgaagc gtttgaaaaa gcaaatccag atgtaaaagt gaaattggaa
    10201 accatcgact tcaagtcagg ccctgaaaaa atcacaacag ccatcgaagc aggaacagct
    10261 ccggacgtac tctttgacgc accaggacgt atcatccaat acggtaaaaa tggtaaattg
    10321 gctgagttga acgacctttt cacagatgaa tttgttaaag atgtcaataa cgaaaacatc
    10381 atacaagcaa gtaaagctgg agacaaggct tacatgtatc caattagttc tgccccattc
    10441 tacatggcaa tgaacaagaa aatgctagaa gatgctggag tggcaaacct tgtaaaagaa
    10501 ggttggacaa ctgatgattt tgaaaaagta ttgaaagcac tcaaagacaa gggctacaca
    10561 ccaggttcat tgttcagttc tggtcaaggg ggagaccaag gaacacgtgc ctttatctct
    10621 aacctttata gcggttctgt aacagatgaa aaagttagca aatatacaac tgatgatcct
    10681 aaattcgtca aaggtcttga aaaagcaact agctggatta aagacaattt gatcaataat
    10741 ggttcacaat ttgacggtgg ggcagatatc caaaactttg ccaacggtca aacatcttac
    10801 acaatccttt gggcaccagc tcaaaatggt atccaagcta aacttttaga agcaagtaag
    10861 gtagaagtgg tagaagtacc attcccatca gacgaaggta agccagctct tgaatatctt
    10921 gtaaacgggt ttgcagtatt caacaataaa gacgacaaga aagtcgctgc atctaagaaa
    10981 ttcatccagt ttatcgcaga tgacaaagag tggggaccta aagacgtagt tcgtacaggt
    11041 gctttcccag tccgtacttc atttggaaaa ctttatgaag acaaacgcat ggaaacaatc
    11101 agcggctgga ctcaatacta ctcaccatac tacaacacta ttgatggatt tgctgaaatg
    11161 agaacacttt ggttcccaat gttgcaatct gtatcaaatg gtgacgaaaa accagcagat
    11221 gctttgaaag ccttcactga aaaagcgaac gaaacaatca aaaaagctat gaaacaatag
    11281 tccttagtta ttctataaaa agtagttttt aaagaagcta agagtgtata cccccttttc
    11341 cctctacaca gatagtgtaa gaaaaggggg cttttgttta aaatgtaaga aactgtcacg
    11401 aaattaaaat gaagttctta cataagcgaa tcataaaaaa tttcattttg attttaaaac
    11461 agttcaagaa agtcaaaaaa ttattctatt tgaaagagag gtgccgactg tgaaagtcaa
    11521 taaaatccgt atgcgggaaa cagtgatttc ctacgctttc ctagcaccag tattattctt
    11581 ctttgtcatc tttgtgttgg ctccgatggt gatgggcttc attacaagtt tctttaacta
    11641 ctcaataact aaatttgagt ttgtaggctt ggataactat atccgtatgt ttaaagatcc
    11701 tgtctttaca aaatctctga ttaacacagt tattttggtt attggatctg taccagttgt
    11761 tgttctattc tcactctttg tagcatctca gacctatcat caaaatgtca ttgccagatc
    11821 cttctaccgt ttcgtcttct tccttcctgt tgtaacgggt agtgttgccg tgacagttgt
    11881 ttggaaatgg atttatgacc cactatcagg gattctaaac tttgtcctta agtcaagcca
    11941 catcatcagc caaaacattt cttggttggg agataaaaac tgggcattga tggcgattat
    12001 gattattctc ttgaccactt cagttggtca gcccatcatc ctttatatcg ctgccatggg
    12061 gaatattgac aattcactgg ttgaagcggc gcgtgttgat ggtgcaactg agtttcaagt
    12121 tttttggaag attaaatggc caagccttct tccaacaact ctttatattg caatcatcac
    12181 aacaattaac tcattccagt gtttcgcctt gattcagctt ttgacatctg gtggtccaaa
    12241 ctactcaaca agtaccttga tgtactacct ttacgaaaaa gccttccaat tgacagaata
    12301 cggctatgcc aacacaattg gtgtcttctt ggcagtcatg attgctatcg taagctttgt
    12361 tcaatttaaa gtacttggaa acgacgtaga atactaaaga aaggagacag ctatgcaatc
    12421 tacagaaaaa aaccattaac agcctttact gttatttcaa caatcatttt gctcttgttg
    12481 actgtgctgt tcatctttcc attctactgg attttgacag gggcattcaa atcacaacct
    12541 gatacaattg ttattcctcc tcagtggttc cctaaaatgc caaccatgga aaacttccaa
    12601 caactcatgg tgcagaaccc tgccttgcaa tggatgtgga actcagtatt tatctcattg
    12661 gtaaccatgt tcttagtttg tgcaacctca tctctagcag gttatgtatt ggctaaaaaa
    12721 cgtttctatg gtcaacgcat tctatttgct atctttatcg ctgctatggc gcttccaaaa
    12781 caagttgtcc ttgtaccatt ggtacgtatc gtcaacttca tgggaatcca cgatactctc
    12841 tgggcagtta tcttgccttt gattggatgg ccattcggtg tcttcctcat gaaacaattt
    12901 agtgaaaata ttcctacaga gttgcttgaa tcagctaaga ttgacggttg tggtgagatt
    12961 cgtacttttt ggagcgtagc cttcccaatt gtaaaaccag ggtttgcggc tcttgcaatc
    13021 ttcaccttca tcaatacttg gaatgactac ttcatgcagt tggtaatgtt gacttcacgt
    13081 aacaatttga ccatctcact tggggttgcg accatgcagg ccgaaatggc aaccaactat
    13141 ggtttgatta tggcaggagc tgcccttgct gctgttccaa tcgtcacagt cttcctagtc
    13201 ttccaaaaat ccttcacaca gggtattact atgggagcgg tcaaaggata atactctacc
    13261 atgaaattga tctttgtgag gtagagaaat gagaataaaa tatttaaaaa ttggtatctt
    13321 ctaagtatgc tgcaagagct agtttcttag atggacaggg gattacagtt gatgagatgg
    13381 cttggataat taatggcatt gtgaatggat tgattggtag ctacataaaa ttaggtactt
    13441 atgcagctaa gtatggtatt agtatggcgc gctcgatctt aagtagggca gctgcaactg
    13501 cggcagcaag agtaggatta ttgactaaga tttttggatg gattttacga atagctgtgc
    13561 tcggagctga tgtatatggt aattttgcca acaatattgc tgtagcttgg gatgcacatg
    13621 ataaaatccc taacaatggt cgtataaact tttaaaatgc gagaatgaaa gcactttgta
    13681 tttttttatt gaatatgtta gcttggacag tgcttgcaat gacagtccgt ggagggctag
    13741 atggatttaa tgagcatact tggagtacta ttttaattgc ttcgctgtcc gggatatata
    13801 tagattatat gtccatagat aaaagtagaa aaaagtccaa aagaaaaaag aaaaaataga
    13861 tttgttcatg gtagggactt atgaaagctt tactgacaaa aaagaaaaca gtttacaaag
    13921 aaaaatgatg gaggagcaaa catggcacaa aaaggagtaa gccttatcaa ggcagcattt
    13981 gatacagaca actttctcat gcgttttagt gagaaagtct tggacatcgt gacagccaat
    14041 cttctttttg tcgtctcttg tttgcccatc gtgacgattg gagtggctaa aatcagcctc
    14101 tacgagacca tgtttgagat taagaagagc agacgggtgc ctgtttttaa aatctatcta
    14161 agatctttca agcaaaatct gaaactaggt cttcagctgg gtttaatgga gttaggaatt
    14221 gtgtttctta ccctttcaga tctctatctt ttctggggtc aaacagctct gcccttccaa
    14281 ttgctgaaag ccatttgttt aggtattctg atttttctta ctatcgtgat gctggctagt
    14341 taccctatcg cggcacgtta tgacctatct tggaaagaaa ttcttcaaaa agggcttatc
    14401 ttggcaagtt ttaactttcc ttggttcttc ctcatgttag ccattcttgt cctcattgtg
    14461 atggttctct atctgtccgc cttcagtcta cttttaggtg gctcagtctt cctgcttttt
    14521 gggtttggac tattggtctt tatccagact ggattgatgg agaaaatttt cgcaaaatac
    14581 caataggagc cttatttctg aaactacttt caaaggctcc aaacgctatt ctataagcga
    14641 gaaactaaaa tctaagtgtc tataagatat tgaaagcctt ttcggtaaag tgtatactaa
    14701 agaagtaaag aaaaatctgc tttagcagaa aataaaaata ataggagaaa atctatgtca
    14761 gatttgaaaa aatacgaagg tgtcattcca gccttctacg catgttatga tgagcaagga
    14821 gaagtaagtc cagagcgtac gcgtgccttg gttcaatact tcattgataa aggtgttcaa
    14881 ggtctttatg tcaatggttc ttctggtgaa tgtatctacc aaagcgttga agatcgcaag
    14941 ttgattttgg aagaagtcat ggcggtagcc aaaggtaaat tgaccattat tgcccatgtt
    15001 gcttgcaata atactaaaga tagtatggaa cttgctcgcc atgctgaaag cttgggagta
    15061 gatgctattg caacgattcc accaatttac ttccgcttgc cagaatactc agttgctaaa
    15121 tactggaacg atattagttc tgcagctcca aatacagact acgtgattta taacattcct
    15181 caattggcag gtgttgcttt gactccaagc ctttacactg aaatgttgaa aaatcctcgt
    15241 gttatcggtg tgaagaactc ttctatgcca gttcaagata tccaaacctt tgtgagcctt
    15301 ggtggggaag accatatcgt ctttaacggt ccagatgaac agttcctagg tggtcgcctc
    15361 atgggtgcca aagctggtat cggtggtact tatggtgcta tgccagaact cttcttgaaa
    15421 ctcaaccaat tgattgcgga taaagattta gaaacagcgc gtgagttaca gtatgctatc
    15481 aacgctatca ttggtaaatt gactgctaca catggaaata tgtacggtgt catcaaagaa
    15541 gttttgaaga tcaatgaagg cttgaatatt ggttctgttc gttcaccatt gacaccagtg
    15601 actgaagaag atcgtccagt tgtagaagcg gctgctgcct tgattcgtga aaccaaggag
    15661 cgcttcctct aatctaaaag gaggtattta tgacatacta cgttgcaatt gatatcggtg
    15721 gaaccaacat caagtatggt ttggttgatc aagaggggca acttcttgaa tcgcatgaaa
    15781 tgccaactga ggcgcataag ggtggacctc atatcttaca aaagaccaaa gatatcgtag
    15841 ctagttattt agaaaaaggc ccagtagcag gtgttgccat atcttctgct gggatggtgg
    15901 atccggataa gggtgagatt ttctatgctg ggccgcaaat ccctaactac gcaggcaccc
    15961 agttcaaaaa ggaaatcgaa gaaagcttta ctattccttg tgagattgaa aatgatgtca
    16021 actgtgcagg tcttgctgag gcagtatctg gttcaggcaa gggagcaagt gtgacacttt
    16081 gcttgaccat tggaacaggt atcggtggtt gcttgattat ggataggaaa gtcttccatg
    16141 gttttagcaa ttcagcctgt gaagtcgggt atatgcatat gcaggatgga gcttttcaag
    16201 acttggcttc tacaacagct ttagtgaaat atgtagctga agcccatgga gaagatgttg
    16261 atcagtggaa tggccgacgt atctttcagg aagctactga aggaaacaaa atctgcatgg
    16321 aaggcattga ccgtatggtt gactatctag gaaaaggtct ggcaaatatt tgctacgttg
    16381 ccaatccaga agtggtcatt cttggtggag gtatcatggg gcaagaggct atcctcaaac
    16441 ctaagatccg cacagccttg aaagaggctt tggtaccaag tttagcagaa aaaacacgat
    16501 tagaatttgc ccatcaccaa aatacagcag ggatgttggg tgcatattat cattttaaga
    16561 caaaacaatc ctagtttggc tcagccaaac taggattttc ttacacgttt ttgtctacga
    16621 tagccgttga gttttttatt ttcccagtag ctattaaaga ttttttcctt gctttcgcga
    16681 ttgatttcca aaaagtaggc ataaatcaaa tcgataaaga agagcatagg aagttgagcg
    16741 gatattcgtt ggatatagga gggttggctg tgggtggcta caagaacagt ctctgtatag
    16801 gtctggctat ctttattggg aacacttgta aagagtacag tctttgcccc catctccttg
    16861 gcatctaata gactatctaa aatagaagga gttgagcctg aaagtgagaa gcccagtact
    16921 agacaatttt catccatgat gctggttgtc caggcaaagc cgtcttggtc tgtcaaagct
    16981 tcgcagacca cacctagtcg cataaaacgt aatttcattt cacgggcgac gaggccagaa
    17041 ctccctgttc caaagaagta gatacgctca gcatcttcga ttagctgggc aattcgctct
    17101 agttggattt cgtcaatcaa gtcttgtgtt tgttccctca tattgctata acttctgagg
    17161 actcgtttgg tcagtggact gtgcttggag acttggttgg cttgattttc tgcctgatgt
    17221 tggtattgga aaataaattc tcggtagcca gtaaagccac actttttagc aaagcgggtt
    17281 agtgcggctt gggagatatg taatttctga gtgacttgtt gagaagataa atcatcttta
    17341 atcgtctcag cttgcaaaaa atagcgagca atttcttgct ctaggtctgt catttcttcg
    17401 aagtgggaat caatgacagt tgcgatatcc ggtttgttca tggggaaggc tccttcatac
    17461 gagtcatatt ggaaaaaggc tagtcttaca ttta
//
LOCUS       DAVKDZ010000040        17485 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000040 DAVKDZ010000000
VERSION     DAVKDZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17485
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.041"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            613..2766
                     /locus_tag="VWZ30_001327"
     CDS             613..2766
                     /locus_tag="VWZ30_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9335051.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLFSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGSVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2779..4128
                     /locus_tag="VWZ30_001328"
     CDS             2779..4128
                     /locus_tag="VWZ30_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU9335052.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQSEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQFFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4298..5005
                     /locus_tag="VWZ30_001329"
     CDS             4298..5005
                     /locus_tag="VWZ30_001329"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU9335053.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            5207..8932
                     /locus_tag="VWZ30_001330"
     CDS             5207..8932
                     /locus_tag="VWZ30_001330"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU9335054.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFTQFEAIQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKVG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVCVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEAANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9025..10467
                     /locus_tag="VWZ30_001331"
     CDS             9025..10467
                     /locus_tag="VWZ30_001331"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU9335055.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     CDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDSLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10504..11526
                     /locus_tag="VWZ30_001332"
     CDS             10504..11526
                     /locus_tag="VWZ30_001332"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU9335056.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11523..12068
                     /gene="purN"
                     /locus_tag="VWZ30_001333"
     CDS             11523..12068
                     /gene="purN"
                     /locus_tag="VWZ30_001333"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU9335057.1"
                     /translation="MKKIAVFASDNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12152..12661
                     /locus_tag="VWZ30_001334"
     CDS             12152..12661
                     /locus_tag="VWZ30_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9335058.1"
                     /translation="MLESKQTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12686..14233
                     /gene="purH"
                     /locus_tag="VWZ30_001335"
     CDS             12686..14233
                     /gene="purH"
                     /locus_tag="VWZ30_001335"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU9335059.1"
                     /translation="MTKRVLISVSDKVGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYIKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAANKYGLTMVFT
                     GVRHFRH"
     gene            14355..15617
                     /gene="purD"
                     /locus_tag="VWZ30_001336"
     CDS             14355..15617
                     /gene="purD"
                     /locus_tag="VWZ30_001336"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU9335060.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            16020..16508
                     /gene="purE"
                     /locus_tag="VWZ30_001337"
     CDS             16020..16508
                     /gene="purE"
                     /locus_tag="VWZ30_001337"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU9335061.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16495..>17485
                     /gene="purK"
                     /locus_tag="VWZ30_001338"
     CDS             16495..>17485
                     /gene="purK"
                     /locus_tag="VWZ30_001338"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU9335062.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMY"
BASE COUNT         4848 a         3491 c         4258 g         4888 t
ORIGIN      
        1 ataagaggaa ctttggagat attcttttat ctaaaaatat aaccttaagt tttagcattt
       61 tactttgtta tttatttaat ccatcggata taaagtttgc ctatttcagc gtattgacag
      121 tattactaat tgggataaca aactatgcga gatttaaaaa tttaagattg acagtagtgt
      181 tttctgtacc atttttattt aatgctataa tgtctattat aaatagtttt tagaaatact
      241 aaaatttttt tctttactta ataatgatat gaaatcattt cggttttgtt aacttgtaat
      301 tcttatattt tctaggtaga acgtttgttc tgcctatttt tttatccaaa aagtgcagtt
      361 gggagggaga taggctcatt tgggaaggaa gtccagtttt tgtttagtga ttggggtaag
      421 atagttgtta tcagatgagt ttatactctt cgaaaatcaa attcaaacca cgtcaacgtc
      481 gccttgccgt atatatgtga ctgacttcgt cagtcctatc tacaacctca aaacagtgtt
      541 ttgagcagcc tgcggctagt ttcctagttt gctctttgat tttcattgag tattagggaa
      601 aaggagatga atatgaaatt tgggaaacgt cactatcgtc cgcaagtgga tcagatggac
      661 tgcggtgtag cttcattagc catggttttt ggctactatg gtagttatta ttttttggct
      721 cacttgcgag aattggctaa gacgaccatg gatgggacga cggctttggg cttggtcaag
      781 gtggcagagg agattggttt tgagacgcga gccattaagg cggatatgac gctttttgac
      841 ttgccggatt tgacttttcc ttttgttgcc catgtgctta aggaagggaa attgctccac
      901 tactatgtgg tgactgggca ggataaggat agcattcata ttgccgatcc agatcccggg
      961 gtgaagttga ctaaactgcc acgtgagcgt tttgaggaag aatggacagg agtgactctt
     1021 tttatggcac ctagtccaga ctataagcct tataaggaac aaaaaaatgg tctgctctct
     1081 tttatcccta tattagtgaa gcagcgtggc ttgattgcca atatcgtttt ggcaacactc
     1141 ttggtaaccg ggattaacat tgtgggttct tattatctgc agtctatcat tgatacctat
     1201 gtgccagatc agatgcgttc gacactaggg attatttcta ttgggctagt catcgtctac
     1261 atcctccagc aaatcttgtc ttacgctcag gagtatctct tgcttgtttt ggggcaacgc
     1321 ttgtcgattg acgtgatttt gtcctatatc aagcatgttt ttcacctccc tatgtccttc
     1381 tttgcgacac gcaggacagg ggagatcgtg tctcgtttta cagatgctaa cagtatcatc
     1441 gatgcgctgg cttcgaccat tctttcgatt ttcctagatg tgtcaacggt tgtcattatt
     1501 tcccttgttc tattttcaca aaataccaat ctctttttca tgactttatt ggcgcttcct
     1561 atctacacag tgattatctt tgcctttatg aagccgtttg aaaagatgaa tcgggatacc
     1621 atggaagcca atgcggttct gttttcttct atcattgagg acatcaacgg tattgagact
     1681 atcaagtcct tgaccagtga aagtcagcgt taccaaaaaa ttgacaagga atttgtggat
     1741 tatctgaaga aatcctttac ctatagtcga gcagagagtc agcaaaaggc tctgaaaaag
     1801 gttgcccatc tcttgcttaa tgtcggcatt ctctggatgg gggctgttct ggtcatggat
     1861 ggcaagatga gtttggggca gttgattacc tataatacct tgctggttta ctttaccaat
     1921 cctttggaaa atatcatcaa tctgcaaacc aagcttcaga cagcgcaggt tgccaataac
     1981 cgtctaaatg aagtgtatct agtagcttct gagtttgagg agaagaaaac agttgaggat
     2041 ttgagcttga tgaagggaga tatgaccttc aagcaggttc attacaagta tggctatggt
     2101 cgagacgtct tgtcggatat caatttaacc gttccccaag ggtctaaggt ggcttttgtg
     2161 gggatttcag ggtcaggtaa gacgactttg gccaagatga tggttaattt ttacgaccca
     2221 agtcaagggg agattagtct gggtagtgtc aatctcaatc agattgataa aaaagccctg
     2281 cgccagtaca tcaactatct gcctcaacag ccctatgtct ttaacggaac gattttggag
     2341 aatcttcttt tgggagccaa ggaggggacg acacaggaag atatcttacg ggcggtcgaa
     2401 ttggcagaga ttcgagagga tatcgagcgc atgccactga attatcagac agaattgact
     2461 tcggatgggg cagggatttc aggtggtcaa cgtcagagaa tcgctttggc gcgtgctctc
     2521 ttgacagatg cgccggtctt gattttggat gaggcgacta gcagtttgga tattttgaca
     2581 gagaagcgga ttgtcgataa tctcatagct ttggacaaga ccttgatttt cattgctcac
     2641 cgcttgacta ttgctgagcg gacagagaag gtagttgtct tggatcaggg caagattgtc
     2701 gaagaaggaa agcatgctga tttgcttgca cagggtggct tttacgccca tttggtcaat
     2761 agctagaaag aggagaggat gaaaccagaa tttttagaaa gtgcggagtt ttataatcgt
     2821 cgttaccata atttttccag tagtgtgatt gtacccatgg cccttctgct cgtgttttta
     2881 cttggctttg caactgttgc agagaaggag atgagtttgt ccactagagc tactgtcgaa
     2941 cccagtcgta tccttgcaaa tatccagtca actagcaaca atcgtattct tgtcaatcat
     3001 ttggaagaaa ataagctggt taagaagggg gatcttttgg ttcaatacca agaaggggca
     3061 gagggtgtcc aagcggagtc ctatgccagt cagttggaca tgctaaagga tcaaaaaaag
     3121 caattggagt atctgcaaaa gagcctgcaa gaaggggaga accactttcc agaggaggat
     3181 aagtttggct accaagccac ctttcgcgac tacatcagtc aagcaggcag tcttagggct
     3241 agtacatcgc aacaaaatga gaccatcgcg tcccagaatg cagcagctag ccaaacccaa
     3301 tccgaaatcg gcaacctcat cagtcaaaca gaggctaaaa ttcgcgatta ccagacagct
     3361 aagtcagcta ttgaaacagg tgcttccttg gccggtcaga atctagccta ctctctttac
     3421 cagtcctaca agtctcaggg cgaggaaaat ccccaaacta aggttcaggc agttgcacag
     3481 gttgaagcac agatttctca gttagaatct agtcttgcta cttaccgtgt ccagtatgca
     3541 ggttcaggta cccagcaagc ctatgcgtca gggttaagca gtcaattgga atcccttaaa
     3601 tcccaacact tggcaaaggt tggtcaggaa ttgacccttc tagcccagaa aatcttggag
     3661 gcagagtcag gtaagaaggt acagggaaat cttttagaca aggggaaaat tacggcgagt
     3721 gaggatgggg tgcttcatct taatcctgag accagtgatt ctagcatggt tgcagaaggt
     3781 gccctactag cccaacttta tccatctttg gaaagagaag ggaaagccaa actcacagcc
     3841 tatctaagtt caaaagatgt agcaagaatc aaggtcggtg attctgttcg ctatactacg
     3901 actcatgatg ccgggaatca attttttcta gattctacta ttacaagtat tgatgcgaca
     3961 gctactaaga ctgagaaagg gaatttcttt aaaatcgagg cggagactaa tctaacttcg
     4021 gagcaggctg aaaaacttag gtacggggtg gaaggccgct tgcagatgat tacgggcaag
     4081 aaaagttatc tacgttatta tttggatcaa tttttgaaca aagagtaatg ttcgtgtttt
     4141 tagagttaaa taatttttaa actgtgagaa agattcttct tgcagttttt tctttacaat
     4201 ttttgaaaaa catatgctat ttattcggtt aaattcttgt gttttttggt tttttgtggt
     4261 aaaatgtgct caagtaatac gaaaggcgaa ctttaaaatg tcaaaacaat tgatctattc
     4321 gggaaaagct aaagatatct atacaactga ggatgaaaat cttattattt caacttacaa
     4381 ggaccaggcg actgctttca acggtgtcaa gaaggagcag attgcaggta agggagtctt
     4441 gaataatcag atctcatctt ttatttttga gaaattaaat gcggctggtg tggcgactca
     4501 ctttgtggag aaactttcag acacggaaca actcaataaa aaggttaaga ttattccttt
     4561 ggaagtcgtg ctccgcaact atactgctgg ttccttttca aaacgttttg gtgtggatga
     4621 gggaatcgcc ttggagactc cgattgtcga attttactac aaaaatgatg atttggatga
     4681 tccatttatc aatgatgagc atgtgaaatt cctacagatt gcgggtgacc agcagattgc
     4741 ctacttgaag gaagaaacgc gtcgtatcaa tgaactattg aaagtctggt ttgctgagat
     4801 tgggcttaaa ttgattgact ttaagctaga gttcggtttt gacaaggatg gcaagattat
     4861 cttggcagac gaattttcac cagataactg ccgcttgtgg gacgctgatg gcaaccacat
     4921 ggataaggat gttttccgta gaggattggg agaactaacc gacgtttatg agattgtttg
     4981 ggaaaagttg caggaattga aataatctgt ttgcaacgga aaaccttcgt ctctcaacta
     5041 aaaggactca ggctgaaaag gtcccccaga ccttttcact ctgtagagaa ctaggtgaac
     5101 taacagatgt ttacgaaatt gtctgggaaa agttgcaggg tttaaaataa caacctcaag
     5161 gctgtttggg aatattgcaa gagctgaaat aaaggaataa gaattgatgg ataaacgtat
     5221 ttttgttgaa aaaaaggctg attttcaggt caagtcagag agtttggtta gagagctcca
     5281 gcacaacttg ggactgtcaa gcttgaaaag tattcgtatt gtgcaagtat atgatgtatt
     5341 tgacttggct gaggacttgt ttgcacctgc agagaagcac attttctctg agcagggaac
     5401 cgaccatgtt ttagatgaag tatctgtgca ggcggatctt gctaactatg ctttctttgc
     5461 cattgaaagt ctgccagggc agtttgacca gcgtgcagct tcgtcacagg aagccttgct
     5521 tttgttggga agttcgagtg acgtgacagt caacacagcc caactttact tggtgaataa
     5581 agatattgat gcgactgagt tggaagctgt caaaaactac ctgctcaatc cagttgattc
     5641 tcgtttcaag gatatcacga cagggattgc caagcaggag ttttcagagt cagacaagac
     5701 cattccaaaa ttgactttct ttgaaagcta tacagcagaa gactttgctc gctacaaggc
     5761 cgagcaaggg atggccatgg aagtggatga tttgctcttt atccaagact actttaagtc
     5821 aatcgggcgc gtgccaactg agactgaact caaggttttg gacacttact ggtctgacca
     5881 ctgccgtcat acgacttttg agacagagtt gaaacacatc gacttttcag cttctaaatt
     5941 tcaaaagcaa ttgcagtcaa cctatgacaa gtatattgcc atgcgcgagg agttaggtcg
     6001 gtctgaaaaa ccacaaacct tgatggatat ggcgactatt ttcggtcgtt atgagcgtgc
     6061 taatggacga ttggatgata tggaagtctc tgacgaaatc aatgcctgct cagttgaaat
     6121 tgaagtggat gttgatggtg tcaaggaacc ttggctcctc atgtttaaaa atgaaaccca
     6181 caaccatcca acagaaattg agccatttgg tggagcggct acctgtatcg gtggagctat
     6241 tcgtgatccg ttgtcaggcc gttcctatgt ttaccaagcc atgcgtattt caggtgctgg
     6301 tgatattaca gcaccgattt cggaaactcg tgctgggaaa ttgccacaac aagtcatttc
     6361 taaaacagca gctcatggtt attcttcata tggtaaccag attgggcttg ccacaaccta
     6421 cgttcgtgaa tacttccacc caggctttgt agctaaacgt atggaacttg gtgccgttgt
     6481 tggtgcggct cccaagggca atgttgtccg tgaaaaacct gaagcaggtg atgtgatcat
     6541 ccttctcgga ggcaaaacag gtcgtgatgg tgtcggtggt gcgacgggct cttctaaggt
     6601 tcaaacagtt gagtctgtag agactgctgg tgctgaggtt caaaaaggaa atgccatcga
     6661 agaacgcaag attcagcgcc tcttccgtaa tggcaatgtc actcgtctga tcaagaagtc
     6721 caatgacttt ggggcaggcg gcgtctgtgt ggctatcggt gaattggcag acggtcttga
     6781 aatcgacctc aacaaggtgc ctcttaaata ccagggcttg aatggtacag aaattgccat
     6841 ctctgaatca caagaacgga tggcggtcgt ggttcgtcct gaagatgtgg ataccttcgt
     6901 tgccgaatgt aacaaagaaa atattgatgc tgttgtggtg gcgacagtaa ctgaaaaacc
     6961 aaatcttgtc atgcactgga atggtgagac aatcgttgac ttggagcgtc gtttccttga
     7021 caccaatggt gtgcgcgtgg ttgtcgatgc caaagttgtg gacaaggatg tcaaactccc
     7081 agaagagcgt caaacatctg ctgaaacact ggaatcagat acccttacgg ttctatctga
     7141 cctcaaccat gcaagtcaaa aaggattaca gactatcttt gactgctctg ttggacgctc
     7201 aacggttaat cacccacttg gtggtcgtta ccaactcaca ccaactgagg catctgtgca
     7261 gaaattgcca gttcaacacg gtgtgactca tactgcgtcg gtcattgctc aaggtttcaa
     7321 cccatatgta gctgaatggt ctccatacca cggtgctgct tatgcggtta tcgaagcaac
     7381 tgctcgtttg gtggctgctg gtgccaactg gtccaaggct cgtttctctt accaagagta
     7441 tttcgagcgt atggataaac aagcagagcg tttcggtcag ccagtagctg ctcttctagg
     7501 ttctattgaa gcacaaattc agcttggctt gccatctatc ggtggtaagg actccatgtc
     7561 tggtaccttt gaagaattga ccgttccgcc aaccttggtt gcctttgggg tgacgacggc
     7621 agatagccgt aaggtgctct ctccagaatt taaagctgtt ggggaaaata tctactacat
     7681 cccaggtcaa gccctctctg cagagattga ttttgacttg attaagaaaa attttactca
     7741 gtttgaagcc atccaagctg accataaagt gacatctgca tcagctgtca aatacggtgg
     7801 tgtagttgaa agtttggctc ttgctacctt tggaaactat attggtgcag aggtgacctt
     7861 gcctgaactt aaaacagctt tgacagctca attaggcggc tttgtcttca catctcctga
     7921 agaaattgct ggagtagaga aggttggaca aacgaaagca gactttacac tgactgtcaa
     7981 cggtgtgaag ctagatggac acaagcttga cagtgcattt caagggacat tggaagaagt
     8041 ttacccaaca gaatttaccc aagcgaaaga actagaagaa gtaccagctg tggcatcaga
     8101 tgttgtgatt aaagccaaag aaaaggttga aaaacctgtg gtttacatcc cagtctttcc
     8161 aggaaccaac tcagaatatg attcagctaa ggccttcgaa aaagaaggtg cagaggtcaa
     8221 tttggtgcca ttcgtgacct tgaatgaaga agctattgtc aagtcagttg aaactatggt
     8281 tgacaatatc gacaagacta atattctctt ctttgctggt ggattctcgg ctgcggatga
     8341 accagatggt tcagctaagt ttatcgtcaa tatcctgctt aatgaaaaag tgtgtgtggc
     8401 tattgatagc tttatcgccc gtggtggttt gattatcggt atttgtaatg gattccaagc
     8461 cttagtcaaa tcgggtctcc taccctacgg aaactttgaa gctgctaaca gtactagccc
     8521 aaccctcttc tacaatgatg ccaaccaaca cgtggccaag atggtggaaa ctcgcattgc
     8581 caataccaac tcaccatggt tggctggtgt gcaagtgggc gatatccacg ctattcctgt
     8641 ttcgcacggt gaagggaagt ttgtcgtgac ggctgaggaa tttgcagagc tccgtgacaa
     8701 tggacaaatt ttcagccaat acgttgactt taacggtaaa ccaagtatgg attctaagta
     8761 caatccgaat ggttctgtcc atgccatcga aggaattacc agcaagaatg gtcaaatcat
     8821 cggtaagatg ggccactcag aacgttatga ggatggtctt ttccaaaata tcccaggcaa
     8881 taaagaccaa cacctgttcg catcagcggt taaacatttc actggaaaat aagacttaca
     8941 gattttctaa tagatagtat cagtaatgta aaagtcatgt aaatctagct cttgatgatt
     9001 acaaatgaaa attaggtata aaaaatgaca tacgaagtaa aatctcttaa tgaagaatgt
     9061 ggtgttttcg gtatttgggg acatccagat gctgctaagt tgacctattt tggactccac
     9121 agtcttcaac accgtggtca ggagggggca ggaatcctct ccaatgatca aggacaactg
     9181 aagcgccatc gtgacatggg gcttttatca gaagttttca gaaatccagc taatttggat
     9241 aaattgacag gagctggtgc gattgggcat gtgcgttatg cgactgctgg cgaagcttct
     9301 gttgataaca tccaaccctt cctcttccgt tttcacgata tgcagtttgg tttggctcat
     9361 aatggaaatc tgaccaatgc agcctctctc aagaaagaac tggaacaaag aggagcaatt
     9421 ttcagcgcga cttcggactc tgaaatcttg gctcacctca ttcgtcgcag tcataatcct
     9481 agcctgatgg gcaaaatcaa ggaagcgctc agccttgtca aaggtggttt tgcctatatc
     9541 ttgctgtttg aggacaagtt gattgcggct cttgacccta atggctttcg tccgctttct
     9601 atcgggaaaa tggccaacgg agcggtggtt gtttcctctg aaacctgtgc ttttgaggtc
     9661 attggtgccg agtggatttg tgatttgaag ccaggtgaga ttgtgatcat tgatgacgag
     9721 ggcattcagt atgacagcta tacagatgat acccagttgg cgatttgttc tatggagtat
     9781 atctactttg cccgccctga ttctaatatc cacggtgtca atgtccatac ggcacgtaag
     9841 agaatgggag cgcaattggc gcgagaattt aagcatgagg cagatattgt agttggtgtg
     9901 cccaattctt ccctaagcgc ggctatggga tttgcggaag aatcaggctt accaaatgaa
     9961 atgggtctga tcaaaaacca atacacccag cgaactttta tccaaccgac tcaagaattg
    10021 cgggagcaag gagtgcggat gaaactgtct gctgtttcgg gtgttgtcaa aggcaaacgt
    10081 gtggtcatgg tggatgattc cattgtacgt ggaacaacct ctcgtcgtat cgttcagctc
    10141 ttgaaagaag cgggtgcgac tgaggttcac gttgccattg gaagtcctgc actagcgtat
    10201 ccatgtttct acgggattga tatccagacc cgtcaggagc tgattgcagc caatcatacg
    10261 gtcgaagaaa ctcgccaaat cattggtgcg gacagtctga cttatctttc aattgatagc
    10321 ttgattgagt cgattggtat cgaaacagat gcgccgaacg gtggtctctg tgtcgcttac
    10381 tttgacggtg actacccaac gcctctctat gactacgaag aagactatcg tagaagtttg
    10441 gaagaaaaga ccagttttta caagtaggcg acagattctc cattaaagaa aaggaaaaaa
    10501 caaatgacaa ataaaaatgc atatgcccag tcgggtgtgg atgttgaagc gggttatgaa
    10561 gttgttgaac ggattaaaaa gcacgtggcc cgtacggagc gtgcaggtgt catgggagct
    10621 cttggtggct ttggtggtat gtttgacctt tccaagactg gggttaaaga acccgtcttg
    10681 atttcaggga ctgacggtgt cggaaccaag ctcatgttgg ctatcaagta cgacaagcac
    10741 gataccatcg ggcaggactg tgtggccatg tgtgtcaacg acatcattgc tgcaggtgcg
    10801 gaacccctct attttctcga ctacgtagcg acagggaaga atgaaccagc taagctagaa
    10861 caagtggttg ctggtgtggc agaaggttgt gtgcaggctg gtgctgccct catcggtggg
    10921 gaaacggctg aaatgccggg catgtacggc gaagacgact atgacttggc tggttttgcg
    10981 gtcggtgtgg ctgaaaaatc tcaaatcatt gacggttcaa aggtggtaga gggagatgtt
    11041 cttctcggac ttgcttcaag tgggattcac tcaaatggtt actctttggt tcgtcgtgtc
    11101 tttgcggatt acacaggtga ggaagtccta ccagaattgg aaggcaagaa acttaaggaa
    11161 gttctacttg agccgactcg tatctatgtc aaggctgtct tgccgctcat caaagaagag
    11221 ttggtcaacg gcattgccca catcacaggt ggtggcttta tcgaaaatgt ccctcgtatg
    11281 tttgcagatg acctagctgc tgaaattgat gaaagcaaag ttccagtgct tccaattttc
    11341 aaagcccttg aaaaatatgg tcagattaaa cacgaagaaa tgtttgaaat cttcaatatg
    11401 ggtgtgggac ttatgttggc ggtcagccct gaaaatgtag agcgtgtaaa agaattgttg
    11461 gatgaagcag tctatgaaat tggtcgcatc gtcaagaaag aaaacgaaag tgtcattatc
    11521 aaatgaaaaa aatagcggtt tttgcctctg ataatggctc aaattttcag gtgattgccg
    11581 aagaatttcc agtggagttt gtcttttcag accatcgtga tgcctatgtg cttgagcgtg
    11641 caaagcagct cggcgttctg tcctatgctt ttgaactcaa ggagtttgag agcaaggcag
    11701 actacgaagc agcccttgtc gaactcttgg aagaacacca gattgacttg gtttgcctag
    11761 caggctacat gaaaatcgtt ggaccaacct tattgtcggc ttatgaaggt cggattgtca
    11821 acattcatcc agcctacttg ccagaatttc caggagctca tgggattgag gatgcttgga
    11881 atgctggcgt gggtcagtct ggtgtgacca ttcactgggt ggattcgggt gtggatacag
    11941 gccaggtcat caaacaggtt cgtgtgccac gactagctga tgataccatt gacagatttg
    12001 aagctcgcat acatgaagca gagtacaggt tgtatccgga agtagtgaag gctctattta
    12061 cagattgact ttttgatgat tcatatgata tctttgattt taaattggag tcagtgtttg
    12121 ttgaagacgg cttcaaacgg aggtatttgt aatgttagaa tctaaacaaa caactcgata
    12181 tgtattttat gtctatctga tgttattaac ttggggaatc ttatttaagt ttgaaacaaa
    12241 tcctgaattt atagcatttt tcttagctcc aaggtatatc aattggattc cattttcaga
    12301 accactaata gtcgatggaa aaattgtttt tgctgaaatg ttatttaatc tgatttcctt
    12361 tattccatta ggtgtttgtt tccctttgat aaaaactaat ttatctagtt taagaatagt
    12421 cgggacaggt ttcttgatta gtttattgtt tgagtgttta cagtatattt tagcaatagg
    12481 tataacagat ataacggatt tgactttaaa tacgctaggt gtctgtgtag gcttactgat
    12541 ttatcaaatt tttataagag tgttcaaatc acagactaga aaatggatca atatcttagg
    12601 tatgcttagc cttggttttg cttatcttgt tttactgtta ctgcatttaa ttggtgttta
    12661 actaatgatt aaaaaggaga atataatgac taaacgcgtc ttaatcagcg tctcagacaa
    12721 agtgggcatt gttgaatttg cccaagaact caaaaaactt ggttgggaga ttatctcaac
    12781 aggtggaact aaggttgccc ttgataatgc tggggtggat accattgcta tcgatgatgt
    12841 gactggtttc ccagaaatga tggacggtcg tgtgaagacc ctccacccaa atatccacgg
    12901 agggcttctc gctcgtcgtg acttggatag ccacttggaa gcggctaagg acaacaagat
    12961 tgagctcatt gaccttgtgg tggtcaacct ttacccattt aaggaaacta tccttaaacc
    13021 agatgtgact tatgctgatg cagttgaaaa tatcgatatt ggtgggccgt ctatgcttcg
    13081 ttcagcagcg aaaaatcatg ccagtgttac agttgtggta gatcctgctg actacgctgt
    13141 ggttttggac gaattggcag caaacggcga aacctcttat gaaactcgcc aacgtttagc
    13201 agccaaagta tttcgtcaca cagcggctta tgacgccttg attgcagaat acttcacagc
    13261 tcaagtgggt gaaagcaagc ctgaaaaact cactttgact tatgacctca agcaaccaat
    13321 gcgttacggt gagaatcctc aacaagacgc ggacttttac cagaaagctt tgcctacaga
    13381 ttactctatt gcttcagcca aacagctcaa cgggaaagaa ttgtcattta ataatatccg
    13441 tgatgcagat gctgctatcc gtatcatccg tgacttcaaa gatagtccaa ccgttgtggc
    13501 tctcaaacac atgaatccat gtggaattgg tcaagctgat gacatcgaga ctgcttggga
    13561 ctacgcttat gagtctgacc cagtatctat ctttggtggg attgtcgtcc tcaaccgtga
    13621 ggtggatgct gcgacagctg agaagatgca cggcgttttc ctcgaaatca tcattgcacc
    13681 aagctatacg gatgaagcgc tagctatttt gatcaataaa aagaaaaact tgcgtatcct
    13741 tgccttgcca tttaatgctc aagaggctag cgaagtggaa gcagaataca caggtgtagt
    13801 cggtggactt ctcgtgcaaa atcaagacgt ggtcaaggaa agcccagctg actggcaagt
    13861 ggtgaccaaa cgtcagccaa ctgagacaga agcgactgct cttgagttcg cttggaaggc
    13921 tatcaagtac atcaaatcaa atggtattat cgtgaccaac gaccacatga cacttggtgt
    13981 tggtccaggt caaaccaacc gtgtggcttc tgttcgcctt gccattgacc aagccaaaga
    14041 tcgtctggac ggggcggtcc ttgcttcaga tgccttcttc ccatttgcgg ataacgtgga
    14101 agaaatcgcc aaagcaggaa ttaaggccat catccagccc ggtggctctg tccgtgacca
    14161 agaatccatc gaagcagcga ataaatacgg cttgactatg gtctttacag gtgtgagaca
    14221 ttttagacat taagaagata aaagggaaga aaacagtttc tttccttttt tggcttaaaa
    14281 tgctaactga aacaagatta aaacgaactt ttttgatata atgttggtaa aaaattcgca
    14341 aaagaggttg aggaatgaaa ctgcttgttg tcggttctgg tggtcgtgag catgcgattg
    14401 ctaaaaagtt acttgaatca aaagacgtgg aaaaagtctt tgtagctcct gggaatgatg
    14461 ggatgactct ggatggtttg gaattggtaa atatctctat ttccgaacat tctaaattga
    14521 ttgacttcgc aaagaccaat gatgttgctt ggacctttat cggtccagat gatgcccttg
    14581 ctgctggtat cgtggatgat tttaaccaag ctgggcttaa ggcctttggt ccgactaggg
    14641 ctgcagcgga gctggagtgg tccaaggatt ttgccaagga aatcatggtc aaatatggtg
    14701 tttcgacagc agcctatggc acattttcag atttcgagga agccaaggcc tatatcgaaa
    14761 agcatggtgc gcctatcgta gtcaaggcgg atggcttggc gcttgggaag ggtgtcgtcg
    14821 ttgctgagac ggttgagcaa gcggtcgaag ccgctcatga gatgcttttg gacaataaat
    14881 ttggtgactc aggtgcgcgc gtggttattg aggaattcct tgaaggagag gaattttcac
    14941 tctttgcctt tgtcaatggt gataagttct acatcatgcc aacggctcag gaccacaaac
    15001 gtgcctacga tggcgacaaa gggcctaaca cgggtggtat gggtgcctat gcgccagttc
    15061 cacacttacc acagagtgta gttgatacag cggttgacac cattgtcaag ccagttctag
    15121 aagggatgat taaagaaggt cgcccttatc tgggagttct ttacgcaggg cttatcctga
    15181 cagctgatgg accgaaagtc attgagttca acgctcggtt cggagatcca gaaactcaga
    15241 ttatcttgcc tcgcttgacc tctgactttg ctcaaaatat cacagatatc ctggatagca
    15301 aggagccaaa tatcatgtgg acggacaagg gtgtgactct gggtgtggtt gtcgcatcca
    15361 agggctaccc gctagactat gaaaggggcg ttgagttgcc agcaaagaca gaaggcgatg
    15421 tcatcaccta ctatgcaggg gctaagtttg cggaaaatag cagagcactg ctctcaaacg
    15481 gcggacgagt ttatatgctc gttaccacag cagataccgt caaagaagcc caagccagca
    15541 tctaccaaga actatcccaa caaaaaatag aaggactctt ctaccgaaca gatatcggaa
    15601 gcaaggcaat taagtaaaga tataagaata acgcgccgta gtcgccaaac acgataatgg
    15661 tcgtcgtggt gaaaagacca gaacagtgaa tgttctggtc agggggaaac ttggagacct
    15721 taggctcaaa gtttaggaat gaaaccgaag gtttgcttcc gcctccatca cctaagacca
    15781 ttatcaaaaa gaaaaataaa aattcacaaa atacgttaat gatcgtatgg tttgcgatcg
    15841 ttagcgagct aatatagaac aatcaccgcc gttgtgaaag aacgattgga tgataatcca
    15901 atcgttcagg gaaattggaa gaccttgggt ttccaattta ggcatgagac acctttggtg
    15961 gctgctgccg tccctcacaa gctaaggtga ttgttgaaaa agaggaaaaa ggagaagaaa
    16021 tgaaaccagt aatttccatc atcatgggct caaaatccga ctgggcaacc atgcaaaaaa
    16081 cagcagaagt cctagaccgc ttcggtgtag cctacgaaaa gaaagttgtt tccgcacacc
    16141 gtacaccaga cctcatgttc aaacatgcag aagaagcccg tagtcgtggc atcaagatca
    16201 tcatcgcagg tgctggtggc gcagcgcatt tgccaggcat ggtagctgcc aaaacaaccc
    16261 ttccagtcat tggtgtgcca gtcaagtctc gtgctcttag tggagtggat tcactctatt
    16321 ctatcgttca gatgccgggt ggggtgcctg ttgcgaccat ggctatcggt gaagctggag
    16381 cgactaacgc agctctcttt gccctccgtc tcctctctgt agaagataag tccattgcgg
    16441 atgcacttgc caactttgct gaagaacaag gaaaaatcgc agaggagtcg tcaaatgagc
    16501 tcatctaaaa caatcggaat tatcggtggc ggtcaactgg gtcagatgat ggccatttct
    16561 gctatctaca tgggccacaa ggttatcgcg ctggatcctg cggcggattg cccggcctct
    16621 cgcgtggcgg aaatcattgt ggcaccttat aacgatgtgg acgccctccg tcagttggca
    16681 gaccgttgcg atgtcctcac ttacgagttt gaaaatgtcg acgctgacgg tttggatgcc
    16741 gttatcaagg atggacaact ccctcaagga acagatctgc tccgcatttc gcaaaatcgt
    16801 atttttgaaa aggacttttt gtcaaacaag gctcaagtca ctgtggcacc ctacaaggtc
    16861 gtgacttcta gcctagactt ggcagatatc gacttgtcga aaaactatgt cctcaagact
    16921 gcgactggtg gctacgatgg tcatggacaa aaggttattc gttcagaagc agacttggaa
    16981 gcagcctatg cgctagcaga ctcagcagac tgcgtcttgg aagaatttgt caactttgac
    17041 cttgagattt ctgtcatcgt gtcaggaaat ggcaaggagg tgacgttttt cccagttcag
    17101 gaaaatatcc accgcaacaa tatcctgtct aagaccatcg taccagcccg catttctgaa
    17161 agtctagcag acaaggctaa agctatggca gtgcgaatcg cagaacaact caacttgtct
    17221 ggaactctct gtgtggaaat gtttgcgact gatgatgacg ttattgtcaa tgaaatcgcc
    17281 ccacgaccac ataactctgg gcactattct attgaagcct gtgatttctc tcagtttgac
    17341 acccatattc tgggtgttct cggagcacca ttaccagtca tcaaactcca tgcgccagcc
    17401 gttatgctta atgtcctcgg tcagcatgtc gaggctgctg agcaatatgt gacagaaaat
    17461 tcaagcgccc acctccacat gtatg
//
LOCUS       DAVKDZ010000041        17028 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000041 DAVKDZ010000000
VERSION     DAVKDZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17028
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.042"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(124..1395)
                     /locus_tag="VWZ30_001339"
     CDS             complement(124..1395)
                     /locus_tag="VWZ30_001339"
                     /inference="COORDINATES: protein motif:HMM:NF013579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9335063.1"
                     /translation="MTRMKESGIDWIGQIPEEWVISKIKFTTQLNGRIGWQGLTSAEY
                     KEEGPYLITGTDFYNGAINFETAVHIDEKRWSEASQIQVENGDLLITKDGTVGKVAII
                     SGLDDKASLNSGVLRIQTVDEIDRKFLYLVLLSDEFWMWFNYTNSGASTILHLYQNVF
                     DEFTFTFPSKNEQQKIADFLDKKTAQLDKVKALLEEQIQKLKDYRASLIYETVTKGLD
                     KNVPMKDSGIDWIGQVPEGWEISKIRYLVTTRSEKRITDSSIPYIGLENIESQTGKFV
                     ETGIQVDKSENIVVEIGDVLFGKLRPYLRKYWRATFPSTASSEFLVFQSSELDMNFLY
                     YAIQSDNFIEEVNTSTYGSKMPRASWEYIKNMKITFPSSLVEQQKIANLLDENTAQID
                     NLIQIKNQQIKNINKQRQTLIYDYVTGKRRV"
     gene            complement(1388..3496)
                     /locus_tag="VWZ30_001340"
     CDS             complement(1388..3496)
                     /locus_tag="VWZ30_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9335064.1"
                     /translation="MSSFKEKLPLFQANDIQISNDVNTVWSIANTLRGAYRADKYRDV
                     IIPMFVLTRLEAALLPTKDQVVAQYKANPNTPEKVLEKLSGYRYYNVSPFTLENLQND
                     PNAIEENFIAYLDGYSCRVKDIIENLKFKEQVRTLAQSGRLFTVIKKFSQIDLSPSSV
                     DSMRMGYMFEDIIRRFSENEEAGSHYTPREVIALMVNLLLIEADEELFVDKKELKILD
                     MAAGTGGMLATAKSYIRQLNTGATARLFGQEVLPETFGIGRADMLIRQEDSDYFVKVD
                     TLKDPDPFPDTKMSFVIANPPFGQAWGGKDADDGVEDAVKKDHQLFDKTEGRQGRFVA
                     TPGTGDAQLLFHLHALTKLEENGRAAIISNGSPLFSGGTSSGESQIRRYILENDLLEA
                     IVALPGQFFYNTGIGIYIWIYNKNKSPERQNKVQFIDATEEFIPLRKSLGQKRRELSQ
                     DNIRQILRWYDDFQENDHVKIFENQEFLYREFTVMQPLQRRGQISEETIEKAKSVPFL
                     AKLFDEYKYQELLEMEPRSAKDEKKLQDFVAGKIKQEAILTALAKGSSDKTYPDFENF
                     VQVIKDLLGEFKLTPANINALALAMSEMDKTAEVIRTKKKDKANEIADGIVYDKTTKD
                     SEIVKLTEDVETYFAREVYPHVPDAHYWFDEEKGYGAEIPFTRYFYQYQEPESAEKLL
                     AEFYALEAELQTLLEELKHD"
     gene            complement(3712..3966)
                     /locus_tag="VWZ30_001341"
     CDS             complement(3712..3966)
                     /locus_tag="VWZ30_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEU9335065.1"
                     /translation="MLLKFTEDAWADYCYWQTQDKKTLKRINKLIKDIQSDSFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSIAFLSFKDHY"
     gene            complement(3970..4224)
                     /locus_tag="VWZ30_001342"
     CDS             complement(3970..4224)
                     /locus_tag="VWZ30_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEU9335066.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFDPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIVQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(4356..5453)
                     /locus_tag="VWZ30_001343"
     CDS             complement(4356..5453)
                     /locus_tag="VWZ30_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU9335067.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLEQAEGLKVVAMSGNFMQRGEPAI
                     IDKWIRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILARLGIDTLAFGTEEVLD
                     YQKIADLYTEQEAEMEKFVEKLPDSLSYPQKTQAMWKEFADLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVDKDVDFASATALRQHQSDQDFLERFMPSVALFEQA
                     SKVSWDDYFPMLRYQILSNSDLTTIYQVNQEMAVRIKEAIKPAQSVEDLVKVVATKRY
                     TKARVRRLLTYILVQARESDLSEAIHVLGFTEKGRQHLKSLKGQVNLVSRIGKEPWDT
                     MTQKADQIYQLGNPSIAEQNFGRVPIRIETN"
     gene            complement(5463..6209)
                     /locus_tag="VWZ30_001344"
     CDS             complement(5463..6209)
                     /locus_tag="VWZ30_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9335068.1"
                     /translation="MIMATYETFAAVYDEVMDDSLYDKWTDFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRAASAKQKIDFMEGNMLDLSKAGQ
                     YDFVTCYSDSICYMQDEVEVGDVFQEVYNVLNEDGVFIFDVHSTYQTDEIFPGYSYHE
                     NAEDFAMLWDTYEDAAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEVLTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSARWFFVAQK"
     gene            complement(6422..6772)
                     /gene="rsfS"
                     /locus_tag="VWZ30_001345"
     CDS             complement(6422..6772)
                     /gene="rsfS"
                     /locus_tag="VWZ30_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU9335069.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIADNIREKVAQAGFKGSHIEGDATGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEAHSVDLSETL"
     gene            complement(6773..7366)
                     /gene="yqeK"
                     /locus_tag="VWZ30_001346"
     CDS             complement(6773..7366)
                     /gene="yqeK"
                     /locus_tag="VWZ30_001346"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU9335070.1"
                     /translation="MAYQDYINCSREALLEKMAELLPEKRLTHCLGVELAAIELAERF
                     GVDVEKAGLAGLLHDYAKKLSDQEFLDLIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LNLHDSEILRAIEIHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETVHTVEHLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            complement(7366..7995)
                     /locus_tag="VWZ30_001347"
     CDS             complement(7366..7995)
                     /locus_tag="VWZ30_001347"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU9335071.1"
                     /translation="MAIELLTPFTKVELEPEIKEKKRKQIGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIEGLAIETIELERK
                     GISYTYDTMKILTEQHPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(8047..8358)
                     /gene="yhbY"
                     /locus_tag="VWZ30_001348"
     CDS             complement(8047..8358)
                     /gene="yhbY"
                     /locus_tag="VWZ30_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU9335072.1"
                     /translation="MSLTSKQRAFLNSQAHILKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(8572..9678)
                     /gene="yqeH"
                     /locus_tag="VWZ30_001349"
     CDS             complement(8572..9678)
                     /gene="yqeH"
                     /locus_tag="VWZ30_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEU9335073.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFISGNDV
                     LLVGNKKDILPKSVKSGKISQWIMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPRTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASTFYDKHLGTLLTPPNSKEKEDFPRLVQRVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVIRKAII"
     gene            complement(9681..10271)
                     /locus_tag="VWZ30_001350"
     CDS             complement(9681..10271)
                     /locus_tag="VWZ30_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU9335074.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYMPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIRIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(10223..11131)
                     /locus_tag="VWZ30_001351"
     CDS             complement(10223..11131)
                     /locus_tag="VWZ30_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9335075.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(11188..11748)
                     /locus_tag="VWZ30_001352"
     CDS             complement(11188..11748)
                     /locus_tag="VWZ30_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU9335076.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVNPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            11887..13092
                     /gene="sstT"
                     /locus_tag="VWZ30_001353"
     CDS             11887..13092
                     /gene="sstT"
                     /locus_tag="VWZ30_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEU9335077.1"
                     /translation="MKRIIHAWNKASLVKRILIGMLCGGILGLTLPNLSGIGLLGDLF
                     VGGLKAVAPILVFALVANALSQHQKGQDSNMKTVVFLYILGTFAAALVAVVASFIVPI
                     EITLNSANTEIAPPDGIGQVLSNLLLKLVDNPVNALITANYIGILSWAVVFGIAMREA
                     SKNSKELLKTIADVTSKIVEWIINLAPFGILGLVFKTISDKGIGSPANYGILLVLLVT
                     TMLFVALVVNPLIAFFFMRRNPYPLVWNCLRVSGVTAFFTRSSAANIPVNMKLCHDLG
                     LNPDTYSVSIPLGSTINMAGAAITINVLTLAAVNTLGIPVDFATAFVLSVVAAISACG
                     ASGIAGGSLLLIPVACSLFGISNDIAIQVVGGGFVIGVIQDSCETALNSSTDVLFTAV
                     AEYAAARKK"
     gene            13160..14011
                     /locus_tag="VWZ30_001354"
     CDS             13160..14011
                     /locus_tag="VWZ30_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9335078.1"
                     /translation="MSISQRTTKLILATCLACFLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFFLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEALK
                     LVYLDHSDHLFHQTDYHIHYFEMRQRQRRILRNMAQQINTCHLAVSESLILAQLFSKI
                     AGQLSQTNPASDLLDEIERYLEVFRNRSLPKTRDEFETRATLLQLLREAKTFIQVKVD
                     FYQKYGQ"
     gene            14154..14534
                     /locus_tag="VWZ30_001355"
     CDS             14154..14534
                     /locus_tag="VWZ30_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9335079.1"
                     /translation="MTYLEKWFDFNRRQKEIETLLEDTIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTSD
                     GQKTLASLQKAVETSLETGLDFLI"
     gene            14632..14853
                     /locus_tag="VWZ30_001356"
     CDS             14632..14853
                     /locus_tag="VWZ30_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9335080.1"
                     /translation="MIEITYLDASKNERTVTFDSYEDFDRSQQACLIGVADYYPVQKL
                     TYKGYDLDYHGTYGDVFFYLMKQDLSQYN"
     gene            14869..15183
                     /gene="trxA"
                     /locus_tag="VWZ30_001357"
     CDS             14869..15183
                     /gene="trxA"
                     /locus_tag="VWZ30_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9335081.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTADQIKAIVAE
                     LS"
     gene            15488..16023
                     /gene="queF"
                     /locus_tag="VWZ30_001358"
                     /pseudo
     CDS             15488..16023
                     /gene="queF"
                     /locus_tag="VWZ30_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
     gene            complement(16278..16946)
                     /locus_tag="VWZ30_001359"
     CDS             complement(16278..16946)
                     /locus_tag="VWZ30_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU9335082.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
BASE COUNT         4792 a         3828 c         3266 g         5142 t
ORIGIN      
        1 cagtctcttt taataaaaaa tgatgaatat ctactaatga aaaataaatc aataaaaatg
       61 ctggaaaaaa tgcggtaaaa aagtaagcta aacgaaattt taaatcagtt ccaaatagca
      121 atcctacacc ctcctcttcc ctgtcacata atcataaata agagtttgtc gttgtttgtt
      181 aatattttta atttgctgat tcttgatttg tataaggtta tcaatttgag cggtgttttc
      241 atccaaaaga ttagcaattt tttgttgctc aacaaggctt gatggaaaag taattttcat
      301 attttttata tattcccaac ttgctctagg cattttacta ccataagtag aagtattaac
      361 ttcttcaata aaattatcac tttgaatagc atagtagaga aaattcatgt ctaattctga
      421 agattgaaac acaagaaatt ctgaactagc tgttgaaggg aaggtagctc gccaatattt
      481 tctcaaatat ggtctcagct ttccaaataa aacatctcca atttcgacta caatattctc
      541 actcttatca acttgtatac cagtttcaac aaatttccca gtttgacttt ctatattttc
      601 taatcctatg taaggtatag aagagtctgt tattcttttt tcacttcgag tagttactaa
      661 atatcttatt ttagaaatct cccatccttc aggcacctgt ccaatccagt cgattcctga
      721 atccttcata gggacgtttt tatccagtcc ttttgtgact gtttcataaa tcaagctagc
      781 gcggtagtct ttgagttttt gaatctgctc ttccaatagg gctttaacct tatctaactg
      841 agctgttttc ttgtctagga aatcagcgat cttttgttgt tcattttttg agggaaaggt
      901 aaaagtaaat tcatcaaata cattttgata taaatgtaaa atagtgctag ctcctgaatt
      961 tgtatagtta aaccacatcc aaaattcatc tgatagcaaa accaaatata aaaattttct
     1021 atcaatctca tcaacagttt ggattcttaa tacaccacta tttaacgaag ccttatcatc
     1081 aagccctgaa ataatagcta ctttacccac cgtaccatct ttcgtaatta gcaaatcgcc
     1141 attttcaacc tgaatctgtg aagcttctga ccaacgtttt tcatcaatat gtacagctgt
     1201 ttcaaaatta atcgctccat tgtaaaagtc agttcctgta attaaatatg ggccttcctc
     1261 tttatattca gctgatgtta atccttgcca accaattcta ccattaagct gtgttgtgaa
     1321 ttttatttta gaaatcaccc actcttctgg tatctgtcca atccagtcta tgccactctc
     1381 tttcatccta gtcatgtttg agttcctcca agagagtctg tagttctgct tccaaggcat
     1441 agaactcagc taagagcttt tctgcacttt ctggttcttg atattgatag aaatagcggg
     1501 taaaaggaat ttctgctcca tagccttttt cctcatcaaa ccagtagtgg gcgtcaggta
     1561 catggggata gacctcacgc gcaaagtagg tttccacatc ctctgtcagc ttaacgattt
     1621 cactgtcctt ggttgtctta tcgtagacaa ttccatcggc aatctcattt gccttgtctt
     1681 tcttcttggt tcggataacc tctgctgtct tatccatttc actcatagct aaggctagcg
     1741 cattgatatt agcaggcgta agcttaaact ctcccagcaa gtccttgatg acttggacaa
     1801 agttctcaaa gtcaggatag gtcttgtcag aacttccctt agcaagagct gtcaggatag
     1861 cttcttgctt gatcttgcca gctacaaagt cctgtaattt tttctcatcc ttggctgaac
     1921 gtggttccat ctccagcaat tcttggtact tgtattcatc aaatagttta gctaggaaag
     1981 gaacactttt cgctttctca attgtttctt cacttatctg gccacgacgt tgcagaggtt
     2041 gcataactgt aaactctcga tagagaaact cttgattctc aaagattttt acatggtcat
     2101 tttcttgaaa atcatcgtac cagcgaagga tctgacggat gttatcttgg gacaactcac
     2161 ggcgtttttg acctagagat ttacgtagag gaataaactc ctccgtagca tcgataaact
     2221 gaaccttgtt ctggcgctca ggagacttgt ttttattata aatccagata taaatcccaa
     2281 ttcctgtgtt atagaaaaat tgacctggaa gggcgactat agcttctagc aagtcattct
     2341 ctaggatata acggcgaatc tgactttctc ccgatgacgt cccacctgaa aagaggggag
     2401 acccatttga aatgatggct gcacggccat tttcttctaa ttttgtaaga gcatgaagat
     2461 ggaagagtag ttgagcatct ccagttcctg gagtcgcaac aaaacgacct tgacgacctt
     2521 ctgttttgtc aaagagttga tgatctttct taactgcatc ctcaactcca tcatccgcat
     2581 ccttaccacc ccaagcttgg ccgaagggag ggttagcgat gacaaagctc attttagtat
     2641 ctggaaaagg atctggatct tttaaggtat ccaccttcac aaaatagtca gagtcttcct
     2701 gacgaattag catatctgca cgaccaatcc caaaggtttc aggcaaaact tcctgaccaa
     2761 agagtcgggc agtggctcct gtattcaact gacggatata gcttttagct gtagccagca
     2821 ttcccccagt tccagccgcc atgtctagta ttttcaactc ttttttatca acaaaaagtt
     2881 cttcgtctgc ctcaatcaag aggaggttga ccatcagagc gataacctca cgaggggtat
     2941 agtgagaccc cgcttcctca ttttcagaaa atcggcggat gatatcctca aacatgtatc
     3001 ccatacgcat agagtcgact gaacttggag acaaatcaat ttgagagaac tttttaataa
     3061 ccgtgaaaag gcgaccactc tgggccaggg tacggacttg ctctttaaat tttaggtttt
     3121 caatgatgtc cttgacacga caagaatagc catccagata ggctataaaa ttctcctcga
     3181 ttgcatttgg atcgttttga agattttcca acgtaaaagg actaacattg tagtaacgat
     3241 agcctgaaag tttctccaac actttctcag gtgtattggg atttgctttg tattgtgcta
     3301 ccacctgatc cttggttggc agtaaggcag cttctaaacg agtcaggaca aacattggaa
     3361 taatgacatc gcgatactta tctgctcgat aagctcctct aagagtgtta gcgatagacc
     3421 aaaccgtatt gacatcatta gagatttgga tatcatttgc ttggaatagt ggtaattttt
     3481 ctttgaaact agacatttac ttcccctgtt tttattatta acaattctct tcattatacc
     3541 acaaaataca agtgataaag cggattcaag ggaaaaaatc gaagtttata tactaaattt
     3601 ctttactgag tttttgtctt ttgtattagg gattctgtca ttttctaaaa aactttatct
     3661 taaacttact cataaaaatt taaaaaataa aaagcccacc tttcagtaga cttagtagtg
     3721 atctttgaag gacaaaaaag cgatgctatc tccatccatc atataaatca agcgattttc
     3781 tgcatcaata cgccgtgacc aggctccttg gtagtcatac ttgagtggtt ctggttttcc
     3841 tattcctgta aatgaatcac tttgaatatc cttaattagt ttattgattc tttttagtgt
     3901 tttcttatcc tgagtttgcc agtagcaata atctgcccac gcatcttctg taaacttgag
     3961 cagcattcct cactcctcaa taacatggac ttgagtgctt ccagcacgga cttgagccat
     4021 tcctcgcaaa accttgtctg ataactcctt attttgaacg attctcaggg tttcctgaat
     4081 actgtcccat tcgctctttg aaagaactac aatgtcctca tctggatttt tattaaccac
     4141 cgttaaagga tcaaattcat cattgacctt cttcatgtag tcttttaaat gatttcggaa
     4201 tgttgagtaa agaactgctt ccataaccat acctcgtttt acctcttttc cactattata
     4261 cacgaaaaga aagaaattgt cagaaacttg tacaagattt tcttttctat ctatttattc
     4321 gtaaaaaatc tcaaaaagcc tacctttcag tagacttagt ttgtttctat tcttattggc
     4381 actcttccaa aattctgttc tgctatactt ggatttccta gttggtaaat ctggtctgcc
     4441 ttttgggtca tagtatccca aggctctttg ccaattcgac tgactagatt gacctgacct
     4501 ttcaaagact tgagatgttg cctgcctttt tcagtaaatc caaggacatg aatggcttct
     4561 gacaagtcac tttctctagc ttgcaccaaa atataggtca aaagacgtct gacacgcgcc
     4621 ttggtgtaac gcttggtcgc aaccacctta accaagtctt ccacagactg ggctggttta
     4681 atagcctctt taatgcgcac agccatttct tgattaacct gatagatggt ggttaggtct
     4741 gaattggaca agatttgata gcggagcata ggaaaatagt catcccagct caccttactg
     4801 gcctgctcaa agagggcaac agatggcata aagcgttcta agaaatcttg atctgactgg
     4861 tgctgacgga gggccgtcgc cgaggcaaag tccacatcct tgtccacaga atggtaacca
     4921 gccccctgac gctgaatcgg atggagcttg atatttcgtc cagcaaccgc cttggcataa
     4981 gcaagagcaa ggacatgatt gggtgtattg cctgaaaaat caagatctgc aaattctttc
     5041 cacatggctt gggttttctg gggataggaa agagaatcag gcagtttttc cacaaatttc
     5101 tccatctcag cctcctgctc tgtgtataag tcagcgattt tttgataatc cagaacttcc
     5161 tccgtcccaa atgctagagt atcaatgccc aaccgagcca agatatccac agctccttga
     5221 ccgaagaaat ctgctgcctg aacactgact aaaaagggca attctactac cagatctgct
     5281 ccattttcca gcgccatctg ggcccgtatc cacttatcta taatcgctgg ttctccacgc
     5341 tgcatgaaat ttccagacat agccacgact ttcagtcctt cagcctgttc cagcaggtat
     5401 ttgtgtccat tatgaaaagg attaaactcc gcaataatac ccgtgatggt catgctaatc
     5461 tcctacttct gcgcgacaaa aaaccaacgg gcacttgtct ctgtcggctc cttgtcctca
     5521 aaatccgcat atagtttgaa ggacttgaaa ccggcctgtt ccagcaaaat atcataggtc
     5581 agaacctcat aagtccgctc ctcatgcact tcatcgtggc gactaaagga accgtccgcc
     5641 tccttgataa agaaagttag ctcatggacg atggagtgag gtgctgcatc ctcataagta
     5701 tcccagagca tggcaaaatc ttccgcattt tcatggtagg aatagcctgg aaaaatttca
     5761 tccgtctggt aagtcgaatg cacatcaaag atgaaaactc cgtcttcgtt gaggacatta
     5821 tagacttctt gaaaaacgtc ccctacttcc acctcatcct gcatgtagca gattgagtcc
     5881 gagtaacacg tgacaaagtc gtattgacct gccttggaca aatccagcat attaccttcc
     5941 ataaaatcaa tcttttgctt ggctgaagcc gctctctttt cggcaatttt caacatatct
     6001 gcgctcaagt ccagtccagt cacatcaaaa ccagcctgag agaagcgcac tgattgaatt
     6061 cctgtcccac aagccaattc caagagtttc tttctctcct tagtttttgg caaatgacgc
     6121 agagaaaaat cagtccattt gtcgtataaa ctgtcatcca tgacctcatc atagaccgcc
     6181 gcaaaagttt cataagttgc cataatcatg ataccaagag cctccatggt aaacaccatt
     6241 cgccctttac ctttctatcc attttcttct aaaataagca aataaaaccc agttgactac
     6301 aactgagttc aattgtacac tatagtcttt tcgtttgctt ttagtactag gcaacgagtt
     6361 gcaggcataa ctggagttag gcaaaacgag ttaacgccgt aataaaagat aaaggaaaag
     6421 actataaagt ttcagataaa tctacagaat gcgcctcatg ccatagtttt tctaggttat
     6481 agtgggcacg catttcttct gagaagatat gcacgacaac agcaccaagg tccagcaaga
     6541 cccagcctcc agttgcatca ccttcgatat ggctaccttt aaagcctgct tgagctactt
     6601 tttcacggat attgtcagcg atagcatcca actgacggct attcattgaa ctagtgataa
     6661 caaagtagtc tgtcacgctg gtcaaatctt gtacatcaag tgcgaggata tcctctgcac
     6721 gtttctcatc agccgctttc acgactagtt ctagtaattc tttttcgttc atttagtcct
     6781 ctttcaaata gtgcacaaag gcgttatagg tttcaagggt ttggggataa atgggaaatc
     6841 cctgatgagc tagatgctcc acggtatgaa ctgtttcgta ggccaccgcc ttattaagcg
     6901 atagactcgc aatctcacgc gccacgtcca ctccaggaaa ggctcgattg tgctcgatat
     6961 agtctgcgac gtagataact ttgtctaggt cggtcatctg accagcaccg actgtatgga
     7021 tttcaatggc tcgtagaatt tcagaatcat gcaaattcaa atcttcctga atcttgtaaa
     7081 ttccgaccat accatgccag acattattgc cccagttttt gaggtcgggg tctaactgat
     7141 aacggtcaat caagtctaaa aattcctgat ctgacagctt tttagcatag tcatgaagaa
     7201 ggcctgctag acctgctttc tcaacatcta ctccaaatct ctctgctagt tctatggctg
     7261 caagctccac acccaagcag tgggttaaac gtttttcagg tagaagctct gccatttttt
     7321 ccaacaaagc ctcacgggag cagttgatat agtcttgata ggccatcagt agagcccctc
     7381 cttctctatg tagtccagta caggctgagg taggagaaag ttgggtttcc gcccttgagc
     7441 aatgaagtca cgcaccatgc tggatgagat gtccatgaga ggcacatcca cccagataac
     7501 tggataggaa gttccagcct tatagcgcgg gcgctgaacc cctacaaact gaaccatgtc
     7561 aaccagctca tcaattcggt accacttagg cagatagtcc accatgtcgg caccgatgat
     7621 aaagtaataa tccgtatctg gatgttgctc tgtcaagatt ttcatggtgt cgtaggtgta
     7681 ggaaatgccc ttgcgctcca actcaatggt ttcaatggct aagccttcaa tcccctcaat
     7741 cgccaactca agcatcttga gacgatggtg ttcagggatg gtttcctttt tatctacgtg
     7801 aggaggttgg tattcaggca taagtagaac ttggtccagt cccaactgct gccgtacttg
     7861 atccgcaaca atgagatggg cattgtgaac agggttaaaa ttccccccta aaatcccgat
     7921 ttgtttgcgt tttttctcct tgatttctgg ctccaactct accttggtaa agggagtcaa
     7981 taattcgatt gccataggct agtctccttt atttctctgt aaaaacagtt atttggagtt
     8041 tcggtcttag atttctttga ctttcttaga aatcttgcga ttttctttct tgctagattg
     8101 tttaaacaaa atcaagatgc gtcctatttt ttggactgta tccacaccga tttcttcttc
     8161 caaaatttca gctacttcgt ggatgttttc atctgtgttt tgtaagagag taaccttgat
     8221 taattcacgc gcatcaagag cttgacggac gctggttttg atttggtcgt tcagtccatt
     8281 tttcccgatt tggatgatag gtttgaggat gtgtgcctgg ctgttgagga aggcacgttg
     8341 ttttgatgtt aatgacataa ttttcttcct tgttttttga tagttatttt aggtggtgag
     8401 ggacggtcgg aactaatttt ccaaagattt catctttgaa aaatggattt cctgacctca
     8461 caattgagcc ttggtctcaa ttgtgcccct tgccaatcta cagattggca atcacaccac
     8521 ggcaataact atcgctatat gagctcactt tgttcgctca gcgattttgt tttaaataat
     8581 tgcttttcgt atgacgacgg cgacgccttc tggtgcccag acggcgactt ttgctatgcc
     8641 tgttacacga atccaaccta ggcctgagat gactaggtct gtcttatctt taatggtaaa
     8701 gacacgctgg actaaccttg ggaaatcttc tttttccttg ctatttggtg gtgtcagaag
     8761 agttcccagg tgcttatcgt agaaagtact agctccttca agcttgctac ggtggagttt
     8821 gagttcatta tcaaagaaag cagtaaatcc ttgcttttct cctgctataa agtcaaagcg
     8881 tcccaaacca cctaaaaata gggtttgctc aggattaagc tgataggtct ttggcttgat
     8941 ttccttttta ggactgacat acttgaggtt tttggccgtc aagtagtgag ccatctggtg
     9001 gcggtggata attcccggcg tatcgtaaat ataagatccg tcgtcaagcg gaatctctat
     9061 tttgtccaag gttgtccttg ggaagcgtga agtagtgatg acattctgat cacccgtgat
     9121 ttcttggata atagcattga ttagagttga ttttccaacg ttggtcacac cgaccacata
     9181 gacatcgcgg cccttacggt agtgttcaat cttgtcaatg acttccttaa tggcatgttt
     9241 attttgtgct gaagttagga ccacatcgac tggacgaagc ccttcttcat gggcacgttt
     9301 catgatccac tggctaatct taccagactt aactgactta gggaggatat cttttttatt
     9361 tcctaccaag aggacatcat tgcccgagat gaaacgtggt aaacctggga tgacagatcc
     9421 attaaaatca aagatatcaa tgacattgac aactaaggca tcactgtctc ccacctcgtg
     9481 caagagcttg aggaaatcat cgttcgtcaa ctggacatct gtgatttcat tgtagtggcg
     9541 gagacggaaa cagcgttggc aatagacttc gccagtctcc aaaccttttt caagtgccga
     9601 ctggggggta aaaccaagac cagctttatc tgtcgtctga atggttgctc cacaaccaat
     9661 acagagaatt tcttccatag ttagattcct tttttatatg taatcggtcc gtacttttca
     9721 gtaatttttc tcataacacg acgctcacga gttcggttaa tctgcgtttt gattgagtca
     9781 tgttggacca agggtttgac taaaattgac cgaatccctg cacggtgggc tgctcgtata
     9841 tctgtcatga gctggtcgcc aaccatgacc acttcctttt tgtcatagtg gaattccttc
     9901 atggcacggt caataccaaa tgtgaagggc ttcaaggccc agtaaacgta atcaatccca
     9961 aatttctcaa ctgctcgttg aacgcgtttt ttggtgttat ttgacaccac gataatgcga
    10021 atgcccgcgt cccgaaggtc atgtagccat tgcttcatct ctggcgttcc atcagggttg
    10081 ttccaagcaa tgagggtatt atccaaatcg accaaaacag ccttgattcc ctgcgcctgc
    10141 aggcttggga ctgtcagatc atagactgct tccacagcaa aatctggcat ataattttca
    10201 atcgccattc tggctccttt tcttaacttt ttttcgcaat tttccttagt aacaaggata
    10261 aaacaatcca taaacctgca ctagccaaac tgatatagag ccaagcatgg ggctcatctg
    10321 ttaaaggtaa gggaacattc attccgaaaa agcctgtcac gactgccaaa acagctagca
    10381 agactgaaat gatagtcaag gttgtcaaat tgtcattcag attattgttt agaatattgt
    10441 tgtaagaggc tgaaagctgc tgtaaaatct gagagattag gtctgtcatg gataccagct
    10501 gatgagcctc aatcatggca tcatcaaact gttctctctc aatctcatca aaactacgat
    10561 acaaggcatg accttgaata tgctctaaca aaatccgatt ttgtttggca gctgccgtca
    10621 gataaaccat accagtctcc aaatcagaca gggcaaagag gtttttcttg gtagttcgct
    10681 ggcgcaagag gtcattgacc tcatccctac tcttgtccat ctgctcaatg acaggatagt
    10741 aggcattgct gatgatttcc agactggcaa agagaaactt ataaatcgaa agcgtgtcat
    10801 ggctctccag ataacgagtc atctgttcaa tgacataggc gttcttggta ttgctaatgg
    10861 taatcaggcg acgatgctcc acgataaagg tcatgggaaa agtctcatag taggccttgt
    10921 ccttttttac gtctaggaca ttatagataa aggtaaccgt cccactttca cggtggtagt
    10981 ccatgtgggc gcgctcgttt ctatccagtg cgtattcaat ggtttccttg tccaaaccgt
    11041 aaatttcaga aaggtcttct agtttattca actttcctag atctaggtct atccaggtac
    11101 aaccattgcc caactgcttt tctaaaacca tcttttctcc ttattaaact ccctctattg
    11161 taccacaaat tacttaaaat aacaggtcta gtctgtgcgt gagaaattgc taacaactgt
    11221 gatatttcgg ttagggacaa agtgaagggc ttgctcctcg ctacgaagct gtgtcgtacg
    11281 aattcccaca ctgacaacct tacccgatac agtaatcggt ccatttgtca gaacgacctc
    11341 atctcccaca tccagttgac gttcaaagag gatgaaaaag ccattgataa catcagacag
    11401 aaagccttgg gctcccatac caatcgctac cccagcaata ccagctccag ccagcaaact
    11461 agaaactggc aaacctaaaa tcgacaaaat gcagtagagt aaaaagaaat atagcgtata
    11521 attaaacaca ttttctagta aacgtgagat ggttttttgg cgtccgacat catgacgaga
    11581 catttttaga gaggggttga caattctctg caccatggta tgaagcattt ttttagcgat
    11641 ataaaataca atcaaaagca gtaaaagaga aatgaccttg gttagaatat tctcgataat
    11701 ggttgtcaca tctagctttt caatataagc ctgaataaat ttttgcatat aaaactcctt
    11761 tatttcatta taccatattt tcatcagtct cattctccat aaatattcaa aaaatagaca
    11821 gaaaattctt gattttgtct atcatttata ctataatcat aaccactaca caaaaaggag
    11881 attattatga aaagaatcat tcatgcttgg aataaggcaa gccttgtcaa gcgtatcttg
    11941 attggtatgc tttgtggggg aatcctagga ctgacccttc ctaatctctc agggattggg
    12001 ctactcgggg atttattcgt cggaggtcta aaagctgttg ctcctatcct agtctttgcc
    12061 ctcgttgcca atgccctttc ccaacatcaa aagggacaag atagcaatat gaaaacggtt
    12121 gttttcttgt atatcctagg gacttttgcc gctgctcttg tagctgttgt agctagtttc
    12181 atcgtcccta ttgaaatcac cctaaatagt gccaataccg aaattgctcc accagatggg
    12241 attggacaag tcctcagcaa cctcttgctc aaactggttg acaatccagt caatgccctc
    12301 attactgcca actacatcgg tatcctatct tgggcagttg ttttcgggat tgccatgaga
    12361 gaggccagca aaaatagtaa agaattacta aaaactatcg ctgacgtgac ttctaaaatt
    12421 gtcgaatgga tcatcaatct ggctccattt ggaatccttg gtcttgtttt taaaaccatt
    12481 tctgacaagg gaatcggaag ccctgccaac tacggtattt tattggttct attagtaacg
    12541 actatgcttt ttgttgccct tgtggtcaac cccttgattg ccttcttctt tatgagacgc
    12601 aatccttacc ctctagtttg gaactgcctc cgtgtcagcg gtgtgacagc cttcttcact
    12661 cgtagttctg cggctaacat tcctgtcaac atgaaactct gccatgacct tggactcaac
    12721 ccagatacct attctgtttc tatcccactc ggttctacta tcaacatggc cggggcagcg
    12781 attaccatta acgttttgac ccttgctgca gttaacactc ttggaattcc tgttgacttt
    12841 gccacagcct ttgtcctcag tgtggtagca gctatctcag cctgtggtgc ttctggtatt
    12901 gctggaggtt ccctcctcct tatcccagtt gcttgtagcc ttttcggtat ttctaacgat
    12961 attgccatac aagttgttgg gggtggtttt gtgattggtg tcatccaaga ctcatgtgaa
    13021 acagccctta actcttctac agatgtcctc tttaccgccg ttgccgaata cgcagcagcc
    13081 cgtaaaaaat aactcataaa ggcaagcctg ctcaggtctt gtcttttacg cttttattct
    13141 aacttatcag gaaattctta tgtctattag ccaacgtacg accaagctca tcttagctac
    13201 ctgtcttgcc tgttttcttg cttattttct caatctttcc tcagcagttt cggctggaat
    13261 tatcgctctc ttgagcctat ctgatacgcg tagaagtacc ttaaaactgg ctcgcaatcg
    13321 actcttttcc atgctcctag ctctggctat cggtgttcta gcttttcact tgagcggatt
    13381 tcatatctgg agccttggtc tctatcttgc cttctatgtg cctctagcct acaagatggg
    13441 ctgggaaatt ggcatcacac caagtactgt tttggttagc cacctcttgg tacaagagtc
    13501 aacctctcca gaccttctag tcaatgaatt ctttctcttt gctatcggta caggatttgc
    13561 tttactggtc aatctctaca tgccttcacg agaagaggaa atccagcact accacacgct
    13621 ggtggaagaa gccctcaaac tggtctattt agatcactct gaccacctct ttcaccaaac
    13681 cgactaccat atccactact ttgagatgag acagcgacaa aggcgtatcc tgcgaaatat
    13741 ggcccagcaa atcaatacct gtcacctagc tgtcagtgag agcctgattt tagcacaact
    13801 cttttcaaaa attgccggtc aactgagtca gaccaatcct gcttctgatt tgctagatga
    13861 aattgaacgc tatctggaag tctttcgaaa ccgcagtctg cccaagacaa gagacgaatt
    13921 tgaaacccgc gccacccttc ttcaactcct acgtgaagcc aaaaccttca tccaagtaaa
    13981 agttgacttt tatcaaaaat atggacagta aaaaataaaa ccactaaaaa acagagcttg
    14041 ctagctcttt tgaaaagtag tataattatt gcatgcgcaa tcatcttgtg acgcaaagac
    14101 tgtccgtgaa tggactttct tctatttaca actctaaaaa gaaaggagat actatgacct
    14161 atttggaaaa atggtttgac ttcaatcggc gtcagaaaga gatcgaaacc ttgttagaag
    14221 acaccattgc ccagcagagt gaacaaagtc tgaccttgaa agagttctac ctgctctact
    14281 atctggactt ggctgaagaa aaatctctac gccagattga tctgccagat aagcttcatc
    14341 tgagcccgag cgctgtttct cggatggtgg ctcgattgga agctaaaaat tgcggtctgc
    14401 ttagtcgcat gtgttgccat caagatagac gctctagctt tatctgcctg acaagtgatg
    14461 ggcaaaagac actggcctct ctacagaaag ctgtcgaaac aagcttggaa actggtttgg
    14521 atttcttgat ttaagatgat ttcggaagaa tgttgcgtgc gcaagcattt ttcttgacat
    14581 tctcttttac aaggagtaaa ataaagtcat cattaaaaaa ggagttttta aatgattgaa
    14641 attacctatc tagatgccag caagaacgaa agaactgtaa ccttcgattc ttatgaagac
    14701 tttgatcgtt cgcaacaagc ttgccttatc ggcgtcgcag actactaccc tgtccaaaaa
    14761 ttaacttaca agggttatga tttggactat catgggactt atggagatgt cttcttctat
    14821 ctcatgaaac aagatttaag ccaatataac taaaaaagga gaaatacaat ggcaaaggca
    14881 attacagatg caacattcga acaagaaaca aaagacggtt tggtcttagt agacttctgg
    14941 gcaacttggt gtggtccatg tcgtatgcaa ggtccaatct tggacaaatt gtctgaagaa
    15001 ctttcagaag atgtcttgaa aatcgttaaa atggacgtgg atgaaaatcc aaacacagct
    15061 cgtgcttttg gaatcatgtc tatcccaact cttctcttca aaaaagacgg ccaagtagtg
    15121 aaacaagttg ctggtgttca cacagcagac caaatcaagg ccatcgttgc tgaattgagc
    15181 taatcaaacg agagaccaag tactttcttt ggtctctttt ttcttgccct ttgccatttt
    15241 tcaaaaaata tgctagacta tagtagattg aaactagaat agtacacctc tacttctaaa
    15301 acattgttag aaatcgattt gactgtccta atcgttttgt cctgttctta tttcatttta
    15361 ctatataaat agaatattac gatgtttggg atatgccatc gctaaaaaaa ctcgatttgg
    15421 tattttttag ctccctaatg ggagcttttt gcgttctctg aacatttccc attttggaag
    15481 gagtactatg aaacgtcaat caaccttggt cgtctttagt ggcggtcaag attcaacaac
    15541 ctgccttttc tgggccatgc aacactatga aacaatcgaa gctgtcacct ttgcctacgg
    15601 ccaacgtcat cacctcgaaa ttcaaattgc tagagaaatc gctaaggaac agggcattcg
    15661 tcaccatatc ctcgatatgt ctctactggg acaaatcact gctcagccag actttacgac
    15721 gattcatatt tcctacattc ctgataagct ctgtgtcgag tcaaaatccc tcaatatatc
    15781 tatctattta gctaccgaaa ccacggagat ttccacgaaa actgtatcaa caccatcggg
    15841 aaagacttgg tcaacttgct agaccctcgc tatttagaag tctggggaaa attcactccg
    15901 cgcggtggca tttcaatcga cccctactac aactacggta gacctggaac caagtatgac
    15961 ggcttggcag aacaacgcct cttccaacac gatctttatc cagagaaaat tgacaaccgt
    16021 taaactcata ctcaatgaaa atcaaaaagc aaactaggaa gctagctaca ggctgctcaa
    16081 agcagtgttt gaggttatag ataagactga cgaagtcagc ttaaaacact gttttgaggt
    16141 tgcagataga actgacgaag tcagtaacat atacatacgg caaggttaag ctgacgtggt
    16201 ttgaagagat tttcgaagag tatcatacga aaaagccctg ttcctcaaga tgaggagcaa
    16261 ggctttttga gtttcaatta ttcttctgtt ccaaggaaat tttttgcaac aagggctgca
    16321 agaactccac cagcgattgg tgcaaggatg aaaatccaaa cttgttgaag ggctgcgccg
    16381 cccaccaaga cagctggtgc caagctacga gctgggttta ctgaaagtcc agtaatcttc
    16441 aatccgacaa gaatcatcgc catcaatgac aaaccgatta ccaaaccagc aatcgcgcca
    16501 ttgcccttac tttctgaagt cacagtcatg ataaccaaga caaataagaa agttgcgatg
    16561 acttcaaaca agaaaccacc aaagacagtg acaccgtttg ccaaggcatt ttcaccaagg
    16621 ctagcagttg acatacctga attagccaag aggaagaaga ctgcgccaga agcgatgaaa
    16681 gctccaacaa cctgaccaag gatgtagttt acaagttctg aagatgacaa acgtttgttt
    16741 acaaacatag caatcgaaac agccgggttc aagtgagcac ctgaaacagt tccgattgag
    16801 taggctgcca ccacgattgc taaaccaaaa gcaaaggcga ttccaaggtg accaaggcca
    16861 tcaagaccat ttccaaaaac aacagctcct gtcccgacga atacaagcat gaacgtaccg
    16921 attaactcag caacaaattt tttcattttc tttctcctcg taatatataa tcatggggat
    16981 atttcaatcc caagttttat tcgttatcat ttgactattg caaggttt
//
LOCUS       DAVKDZ010000042        16750 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000042 DAVKDZ010000000
VERSION     DAVKDZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16750
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.043"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            38..1756
                     /locus_tag="VWZ30_001360"
     CDS             38..1756
                     /locus_tag="VWZ30_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU9335083.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLTRRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKITNIEAE
                     INAFVK"
     gene            1867..2214
                     /locus_tag="VWZ30_001361"
     CDS             1867..2214
                     /locus_tag="VWZ30_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9335084.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFVGL"
     gene            complement(2492..3328)
                     /locus_tag="VWZ30_001362"
     CDS             complement(2492..3328)
                     /locus_tag="VWZ30_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9335085.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYVDF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTNLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(3341..3970)
                     /locus_tag="VWZ30_001363"
     CDS             complement(3341..3970)
                     /locus_tag="VWZ30_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9335086.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(3980..4621)
                     /locus_tag="VWZ30_001364"
     CDS             complement(3980..4621)
                     /locus_tag="VWZ30_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9335087.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(4767..5996)
                     /locus_tag="VWZ30_001365"
     CDS             complement(4767..5996)
                     /locus_tag="VWZ30_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU9335088.1"
                     /translation="MNNSQQMLQALEEQDLTKAEYYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLMLGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLAAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(5986..7152)
                     /locus_tag="VWZ30_001366"
     CDS             complement(5986..7152)
                     /locus_tag="VWZ30_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9335089.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAISIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VWASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFMPTKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFMIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVISGLYELEGEEVKSEQ"
     gene            7296..8309
                     /locus_tag="VWZ30_001367"
     CDS             7296..8309
                     /locus_tag="VWZ30_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU9335090.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHNGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEEKLSKLYTYHSKTGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIRLSTDGQYLYASNRGHNSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIAVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(8355..8774)
                     /locus_tag="VWZ30_001368"
     CDS             complement(8355..8774)
                     /locus_tag="VWZ30_001368"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU9335091.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDEDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(8785..10191)
                     /gene="atpD"
                     /locus_tag="VWZ30_001369"
     CDS             complement(8785..10191)
                     /gene="atpD"
                     /locus_tag="VWZ30_001369"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU9335092.1"
                     /translation="MSSGKIAQVMGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFAGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKILVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(10277..11155)
                     /locus_tag="VWZ30_001370"
     CDS             complement(10277..11155)
                     /locus_tag="VWZ30_001370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU9335093.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSTAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLSDQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(11171..12676)
                     /locus_tag="VWZ30_001371"
     CDS             complement(11171..12676)
                     /locus_tag="VWZ30_001371"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU9335094.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENAMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(12691..13227)
                     /locus_tag="VWZ30_001372"
     CDS             complement(12691..13227)
                     /locus_tag="VWZ30_001372"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU9335095.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAYPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(13227..13721)
                     /gene="atpF"
                     /locus_tag="VWZ30_001373"
     CDS             complement(13227..13721)
                     /gene="atpF"
                     /locus_tag="VWZ30_001373"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU9335096.1"
                     /translation="MHVTVGELIGNFVLITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(13735..14451)
                     /gene="atpB"
                     /locus_tag="VWZ30_001374"
     CDS             complement(13735..14451)
                     /gene="atpB"
                     /locus_tag="VWZ30_001374"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU9335097.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(14486..14686)
                     /locus_tag="VWZ30_001375"
     CDS             complement(14486..14686)
                     /locus_tag="VWZ30_001375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU9335098.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(14761..15768)
                     /locus_tag="VWZ30_001376"
     CDS             complement(14761..15768)
                     /locus_tag="VWZ30_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9335099.1"
                     /translation="MQNYYTTKGKHLTIDSRCLIERWKKEGKSNREIASLLGKAPQTI
                     HTEIKRGTVRQCLGKGCFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHRGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIAQRPEAINLRLENGHYEIDTVLLTRSKNYCLLVLTDRKSRHQIIRLIPNKSAEVVN
                     QALKLILKQHKILSITADNGTEFNRLSDVFSEEHIYYAHPYASWERGTNENHNRFIRR
                     WLPKGTKKMTPKEVAFIEKWINNYPKKCLDYKSPREDFWMANLNLKFSKMEIIFIKRF
                     Q"
     gene            complement(16225..16587)
                     /locus_tag="VWZ30_001377"
     CDS             complement(16225..16587)
                     /locus_tag="VWZ30_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9335100.1"
                     /translation="MENKESHKSQEIGNLLRVYNRSKREETESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYL"
BASE COUNT         5089 a         3670 c         3035 g         4956 t
ORIGIN      
        1 aactgattaa atgttttaaa aataaaggag aatttgaatg tcttaccaag aaaattacca
       61 gaaatgggtt gattttgtgg agcttcctga ctaccttcgt caagatttgg aaaatatgga
      121 cgaaaaaact aaggaagatg ccttctatac aaatcttgaa tttggtactg caggtatgcg
      181 tggcttggtt ggtgctggta caaaccgtat caacatctac gttgttcgcc aagctactga
      241 aggattggct cgtttgattg agtcaaaagg tggaaacgag aaagaacgcg gtgtcgcaat
      301 tgcctacgat agccgtcact tctcacctga gtttgccttt gaatctgcgg cagttcttgc
      361 taaacacggc atcaaatctt acgtatttga aagccttcgt ccgactccag aactatcatt
      421 tgcagttcgt catctcaact gtttcgcagg tatcatggtc acagccagcc acaaccctgc
      481 tccatttaac ggttacaagg tttacggtga agacggtgga caaatgcctc cacacgatgc
      541 ggacgctttg actacttata tccgtgcaat cgaaaaccca tttgcagttg aagttgctga
      601 tgtggaaact gaaaaagctt ctggcttgat tgaagttatc ggcgaagctg ttgacataga
      661 ataccttaaa gaggttaagg acataaacat caacccagcc ttgattgaag aatttggtaa
      721 agacatgaag attgtctaca caccacttca tggtactggt gaaatgttga ctcgtcgtgc
      781 tcttgcccaa gcaggatttg actctgttca agttgttgaa gcgcaagcaa ctgctgaccc
      841 agacttctca actgtaacat ctccaaaccc agaaagccaa gcagcctttg cccttgctga
      901 agaacttggt cgtcaagttg gtgcagatgt tcttgtcgca actgacccag acgctgaccg
      961 tgttggtgtt gaagttcttc aaaaagatgg tagctacctc aacctttcag gtaaccaaat
     1021 cggtgctatc atggctaaat acatcttgga agctcacaaa aacgctggaa ctcttcctga
     1081 aaatgccgct ctctgcaaat ctatcgtttc aactgacttg gtaacgaaga ttgctgaaag
     1141 ctacggcgca actatgttca acgtcttgac aggtttcaaa tttatcgctg aaaaaatcca
     1201 agaattcgaa gaaaaacaca accacactta catgatggga tttgaagaaa gcttcggtta
     1261 cttgattaaa ccatttgtac gtgataaaga tgccatccaa gccgttcttg tcgttgctga
     1321 acttgctgcc tactaccgtt ctcgtggttt gacacttgct gacggtatcg aagaaatcta
     1381 taaagaatat ggctactacg cagaaaaaac aatctctgtt actctttcag gtgtcgatgg
     1441 tgctgaacaa atcaaagcga ttatggctaa attccgcaac aatgctccaa cagaatggaa
     1501 cgcaacagct atcactgtcg tagaagactt caaggcacaa actgctactg ttgctgacgg
     1561 tactgttaca aacttgacaa ctcctccaag tgatgtgttg aaatacacac ttgctgacgg
     1621 ttcatggatt gccgttcgcc cttcaggtac agaaccaaaa atcaagttct acattgcagt
     1681 tgtaggggaa accaacgaag aatcacaagc taagattact aacatcgaag cagaaatcaa
     1741 tgcatttgta aaataaagac ctataaaaga tgagaccaac caacctcatc ttttttcgta
     1801 tcaaatctaa gcaattttag aaactttatg gcatactaga cttatcaatg aaagcgaggt
     1861 aatctcatgg aacaattttt agataacatc aaagaccttg aagtcactac agttgtgcgt
     1921 gcgcaagaag ctcttgataa aaaagaaact gcaaccttct ttatcggtcg caaaacttgc
     1981 ccttactgcc gtaaatttgc aggtacattg tcaggtgtcg tagctgaaac caaagctcac
     2041 atttacttca tcaatagtga agaaccaagc caactcaatg atttgcaagc attccgctca
     2101 cgttatggaa tcccaactgt accaggtttt gttcatatta cagatggaca aatcaacgtc
     2161 cgttgcgact cttcaatgtc agcacaagaa atcaaagatt tcgtaggatt gtaaaaaatc
     2221 agctaacaag tcttaaaatc acaaaaacgc ataatgtaag gtgcaaaaac cttgatatta
     2281 tgcgttttat tgttcctaaa acagttacat cagcatttca ttacagaaca ttttaatttt
     2341 ggaagtcaaa tcaattttta acaatgtttt agaaatcaca gtgggctatt ccatattcaa
     2401 ttatccagat tcaatcgcta ttatcgtttt cctagattac aaaaaaacta cgttttgcta
     2461 atcaaaacgc agtttcagcc actattttat cttactgtcc ttcttttact tctttggttg
     2521 ctacatcttc tccaaaccat ttttggctga tttcttggaa cttgccgtcc ttgtaaagac
     2581 tagaaaaagc ttcatttatc ttcttaacca agtttgtatc ttccttacgg gctccaaccg
     2641 caaaagcttc tgtttctagt ccaactgtaa agacattata atcgtttaaa acaccttctg
     2701 cttctaaata atagtttgca tagacacggt caatcaatag accatcaatt cgatcgtttt
     2761 tcaaatcaat caaggcttca ttaaaggttt ggtattgatt cgcttcctta ttagcgacaa
     2821 tattcttcaa aatttctgga tttgcttcaa agtccacata accagatgaa ccagcttgag
     2881 ctcctaatgt ctttccagtc atatcctttg cagtcgtgat accagatgat ttcttcgtaa
     2941 ccaatacctg ctcattcttc atatatgagt tactgaaagc caccttttca cggcgttcgt
     3001 ctgtagcgga atagccattc caaatcagat caatcgttcc ttttgtcaat tcagcttctt
     3061 tcaaatccca atcaatcggt tgccaattta ccgtgattcc gtatttttca aaaacagctg
     3121 tagctaaatc aatatcaaat cctgcataag aaccatcttt ctgagcaaat cccattggaa
     3181 caaaagtact atcaaatcca atagtaatag acttgttaga ctggtacttt gaccaattat
     3241 ctccactagt ttcgctagaa tttttcccac aagctactaa gaacaaagca gtcatcagac
     3301 tgactaatac aagcatccat tttttcatcc atttttcctc ctatttaggt tctactttca
     3361 ataatacatc tgcgatattt tcagcaaact gcaaatcatg ggtaaccaca atctgggtca
     3421 tcccaagttc cctattttgc aagattagct tctccacttc caaacgtaat tctggatcca
     3481 gggcagaagt tggttcatcg tagccaatga tttctgggtc aatcatcata gcacgcgcca
     3541 aagccacccg ctgcttttgc ccaccagata gtgagaaagg ataggcctct gcgtgtcctc
     3601 ctagtcctaa ctgttccaag agtccactcg ccttcttctc agcctcttcc tgcttcattc
     3661 ccatggtctt cacaggcgat aaagtcaaat tttccagaac tgatagatga ggaaatagtt
     3721 gaaaatcttg gaagacaaat cccagtagat tgcgcttctg caattcatcc agctctaaag
     3781 gttgtccatt ataaaagatt tgccctgaat caatggtttc aagacctgca agcatacgta
     3841 aaagagttgt cttacctcca ccagaaggtc caacgatagc caggatttgc ttttcaggaa
     3901 tacttagact gaaattagac aggatttgtt tgtctccaaa gactttattg atatttcgta
     3961 attctaacat ggcagcctcc tatctataat aactatactt cttctcaact tttttggaaa
     4021 taattgtcac aatcccaatc aaaatcaaat aaatggctcc tgccaagaac ataggaacta
     4081 gactagcatc gcggttagca gctgttcgac tagccaagat aaggtctgaa attccgagag
     4141 catagaccaa agaagtatcc ttgaccaaac tcataacttc attaaagaca ctaggaagaa
     4201 cgatcttggt cacttggggc aagataatat agcgcactct gtcaaaaggg ctaaacttca
     4261 agaccttggc ggcctcatac tgtcctcttg gaatagtgtc aattccccca cggaaaattt
     4321 ctgcaaagta agctgcatag ttgagaacaa aggcaataat agctgcagga aggcggtcta
     4381 aacgaatccc aatacttggg agcacataat agataaaaat cagttgcaag agtaaggggg
     4441 ttcctcgcat aacccagatg taaatgttaa tcagataatg gaggggcttc caatggactt
     4501 gcaaggcaaa ggcaatcaaa acgcccaagg gaatcgaaaa tagcaagacc agtgcaaaga
     4561 cctgtacagt cgtgctcgca ccattcagta aactcggtaa tatctcaaat aaataagaca
     4621 tactgcacct cctaaaagta attgttccta ttatagcatg aataaacaag attaccaagt
     4681 atttttgtaa aaagattcta ccatatacaa ataaaaaccg tagctatcaa aagagataaa
     4741 ctacagttct atatgtttaa acattcttac aatctctcaa acagttcttg catttgcaca
     4801 tcatctggaa ccagttttaa gtaagtgtga gcatggactt ttgcttcttc aaaatgtccc
     4861 aattcacgca agagatagat atagtgttcc agaaattctg gattgtcctt caaatctcct
     4921 gtcaactctt gataatgctc ataagcagta tccaaatcgt ccatttcttg ataagaacga
     4981 gcaatcatcc acttggtcaa aagattttct ggctcctcac tctgcaagtc tagaatatcc
     5041 tcataacgct cctgctccag ataaatagtg gctaaacgaa gcaagatttc ttctgtatcc
     5101 tcagcgtctt cttttgcagc aagaagataa ttttctgcac cactagcatc atgcaattca
     5161 taagaaaatt gtgaagcagc cagcaagagg cgagtttcaa agggattttt ctctaatcct
     5221 tgcttagcga tacgcagggc ttcttgaact tgatgttcct tatgtaaagc ctgactgtac
     5281 ccatactcat agccttcaaa gtcaggagaa atggtatcaa gctgcttaaa gtagagggtg
     5341 gctttttgat attcttcttg atcaaaataa agactggcca actcaaaagc tgttaagtca
     5401 tcgtattcta actccagggc tttttctaaa aactcagtag ccgtttcaaa tttccctaac
     5461 tgagcatagg caaagccaat tcgttgatag gtggaaatgc ccgtttgctc ataaatcgag
     5521 cgattatcta actgggcata ggcttgaata gccgcttggt aattttccaa ctcactatcc
     5581 aactctgcca aacccaacat caagagagaa tcctctgagt aggtcaaggc ctccaataat
     5641 ttctcacgtg ccacatctgt caaaccttcc agctggtaaa ggtctgcctt caaaaccaaa
     5701 gacgagacat accagtcact gtcagcttgg atttcctcaa gataggtaaa ggcttcttct
     5761 atttgaccat cctcgctagc aattgcagct agattaagat gaacctctgg aaaatcctct
     5821 acaattttca ggtaaatttc cttggcctga ggatagaaac caatcccttc aagataagtt
     5881 gccaactcat acagaagatc acttgaatca ttttctaaag ctttggcgaa ataatactca
     5941 gccttagtta aatcttgctc ctccaaagcc tgtaacatct gttgactatt gttcactctt
     6001 gacttcctca ccctctaatt catataggcc actgattacc ttataccatt cgaaaatggc
     6061 tgaaatgaca accttagcag aggcataaac cggaataccg agcaagactc cccagatacc
     6121 aaacatggat cctgaagtta gcaagacaaa gagaacatta atgggatgga tgtttaattg
     6181 acttcccaaa atcaatggag aaacaaaacg gccttcaata gtttgttcta cgataaagac
     6241 aatcactact ttcaaaagca tgactggccc agcaatcaag cccaatacta gggcaggaag
     6301 catggctagg aagctgccaa gataaggaac caaatttaaa ataccagcag taacacctag
     6361 cgtaaccgca tagcgtagac cgataatctt gaagaaaatc ataaacatta ctgctacaat
     6421 aatagctact gtcacttgcc ctcgaacata gttggacaac tgttgattca catctgacaa
     6481 aacctgtcct acaggttctt tcaattttgt cggcatgaat tgggtcaaat agttacgcaa
     6541 gccttttcca tcacgcaaga gataaaagag catgaaagga acaataatca aggcaacaat
     6601 cacttgagaa gccccgctaa taaaggcact cacccagttg actgcctgag atgaaacctt
     6661 actcgcccaa actgtagcct gacttgagaa gtttgtcaaa acttgttcta actgaggtct
     6721 gaaatcatct ggcaggtgct gggctaccaa tccattaaca atcctatcta tatcttctaa
     6781 gtaaacagga acgtttcttg caaaggtcaa aacctgacgt tgcagatttg gaatggcgac
     6841 tgccaagccc caaatgataa agagagctat gatgacaaag acaatgctga tggctatgac
     6901 gcgattgact ttgtgttttt ccatccaatc aacaatagga ttcaacaaat aatataacaa
     6961 accagacaga atgactggca acatcacaac tgctaaaaag tctaaaacag gtgaaaatag
     7021 aaaactaatc ttacttaaaa taaaaagatt gagtcccaat aataaggtta ccaaaaatac
     7081 cgtaatggcc ttgttatcca aaaaccactt gaaaaaccaa gataggctaa aatgtttctc
     7141 tttttgctcc ataaatactt cctttctatt gttctcccat tataccattt ttgaaacaaa
     7201 ataactcttt tttaaagtgc ttacatctaa ttggatgtct ttaattggct tgtttttgtg
     7261 atataatagt cttatcttta cacagaggta tagtaatgaa agaaactgtt tattttggaa
     7321 cttatacacg tcgtacttct caagggattt acaaggcaga ctttgataca gaaactggtc
     7381 agctttcaaa tctagaactt tttgcagctg agccaagtcc aacctacctt gcctttgacc
     7441 agcaccaaca tttatacact gttggtagcc aagacgataa ggggggaatt gcagcctatc
     7501 aaactgacgg gactgtgtta aatcatgttg ttgaagaagg agctccccac tgttatgttg
     7561 ctgtcgatga aaagcgtgat ttggtttacg cagccaacta tcacaaggga caggtccttg
     7621 tttataaacg ccaggaagat ggtagtcttc tacttagcga tatggatcaa cacaatggcc
     7681 aaggtccaca tgaaaatcaa gcttcccccc atgttcacta tacagattta acacctgacc
     7741 actatctagt gacctgcgac ttgggaactg accaagtcat cacctatgac ctcgatcaag
     7801 aagaaaaatt atctaagctc tatacctatc acagcaagac aggagcaggc tcacgccata
     7861 tcattttcca taaccactat aaaatcgctt atctcatttg tgaactcaat agtactatcg
     7921 aagttttaat ctacgatggc gttggcgaat ttgaacgtat gcaggtcatt tcaactttac
     7981 cagaagctta cgaaggcttt aatggaaccg ctgctattcg tctctctaca gacggtcaat
     8041 acctctacgc ttctaaccgt ggtcataatt ctatcgcagt atataccatc cttgcggacg
     8101 gtagcttaga gttattagaa atcgttccaa cacatggtca gactccacgt gattttgatt
     8161 tgacacccga ccaaaaattt ctcattgctg tccaccaaga ctctgacaat gcaactgtct
     8221 ttaaacgtaa ttgtgacaat ggtcgtctag cagaactctc caacgacttc catgttcccg
     8281 aagcggtctg tatccgtttt gctccttaag aaaacataaa aaatgaactt ggaattttca
     8341 agttcatttt tttcttatag tctatttccg acattgatac ggttaatggc acgttgcagt
     8401 gcaatcttcg cacgacgttc ttggtcaatc aagtgtttgt cttgtgcttc ttcaattgca
     8461 cgttctgcac gaagtttggc acgttctgca cgactgatat cgatatcacg agcacgttct
     8521 gcagagtcag cgacgattgt aatcatatca ttggcaattt caataacacc gccgtttact
     8581 gcaatccagt tcacgtgatc ttcgtcatca atacgtttta cctttacttc atcaactgct
     8641 aaaaccgcaa tcatattttc atgtcgtggc aagatcccca tctcaccatc cagagttcga
     8701 accgatacat agctggcatg gtgatcatag acgaggccat ctggtgtcac gatctggaca
     8761 gttaactgag ccatagatca cctcttaaaa tcccattttt tcagcttttg caatcacatc
     8821 ttcgatagaa cctacaccac ggaaggcatc ttctggcaag tggtcgtatt taccatcaag
     8881 gatttcctta aagccacgta cagtttcagc aactggaaca taagaacctg gctgaccagt
     8941 aaattgttct gcgacgttga agttttgtga caagaagaac tggatacgac gggcgcgagc
     9001 aaccaagatc ttttcttcat cagaaagctc atccatacca aggatagcaa tgatatcttg
     9061 caattcatgg taacgttgaa ggacacgttt tacttcagca gcaactgcat agtgctcttc
     9121 tccaacgatt tcaggtgcca aggcacgtga gcttgaagca agtgggtcaa cggctgggta
     9181 gatacccaat tgtaccaact tacgttccaa gtttgttgtt gaatccaagt gagcgaaggc
     9241 tgttgctggc gctgggtcag tatagtcatc cgctggcaca tagatagcct ggatagaggt
     9301 tacagaaccc ttcttggttg atgtgatacg ttcttgcaat tgacccattt ccgtagcaag
     9361 tgttggttgg taaccaacgg ctgatggcat acgacccaaa agggcagata cttctgaacc
     9421 agcctgagtg aaacggaaga tattatcgat aaagagaagc acgtcttggc cttccacatc
     9481 acggaagtat tcagcgattg tcaaaccagt aagggcaaca cgcatacgtg ctcctggtgg
     9541 ctcattcatc tgaccaaaga ccatggctgt tttctcgata acgcctgatt ctttcatttc
     9601 ccagtaaagg tcattcccct cacgagtacg ttccccaaca ccagcaaata ctgaaatacc
     9661 accgtgctct tgggcaatgt tgtgaatcaa ttcttggatt aagacggttt taccaactcc
     9721 ggcaccaccg aaaagtccaa ctttaccacc tttaaggtaa ggggcaagaa ggtcaataac
     9781 cttgatccct gtttcaagga tttcagaaga ggtagacaac tcatcaaaag ttggagcttt
     9841 tttatgaatt ggctgacgct ctgcgtcttc tgtaaaagga gcttccaagt caatggtatc
     9901 tcccaaaacg ttgaagacac gtcccaaagt ttctttacct actggtacag agattggacg
     9961 acctgtgtcc aatacttcca ttccacgagt caacccatct gttgattcca tggcgatagt
    10021 acgaaccata ccatctccta actccaaggc tacttcaagg acgatttttg tttttctttc
    10081 gtcatttttg tagacgacaa gtgcattatt aatctcagga agtttttccc ctgctgcaaa
    10141 caaaacgtct acaacgggac ccataacctg agcaatttta cctgaactca tctccttctc
    10201 ctattctata taagatactg tcggttccta gctcaactag gtcctaagtt cattttcata
    10261 cgagatggac tagagcctat tctaaggcac tagcacctgc tacgatttct gtaatttctt
    10321 gtgtaatcgc cgcctgtctg gcacggttat actgaattgt caaatcattg atgactttct
    10381 tagcattatc tgtcgctgtt tgcatggctg tcatacccgc agcattctca gctgtcttgg
    10441 catcgataat ggcaccgtaa atcatacttt ctgcaaactg aggcaacaac tgctccagaa
    10501 tttcttctcg gctggtttcc aattcaaaag tcaagctgta ctcttcatcc gcttcatttg
    10561 gatccaagtc aacaatcgga agcatttgtt ccacacgcat ttgactggtt agcgtattga
    10621 catggtggtt gtagcaaaca taaagctcat caaagagttc attttggtac atttcaacag
    10681 tttttgaaat aatcttacga acttgatcaa agctaggttg gtctgacaag ccacgtaatt
    10741 cataaagtgg ttgaataccg cgagccttaa agaaatcagc tcccatccca ccgatacaga
    10801 tcatttcaaa acctttaccg tctgggtggt attcttcttt caactccata acagctttca
    10861 aaatagagga attataacct ccaaccaaac cgcggtctga agtgataacg atatagcctg
    10921 tcttcttcac agaacggcta atcaacatcg gattagttga agcaccagct ccattaccat
    10981 gaaggatatc tgtcaaaagt ttacgcactt tctgagcgta aacttggaag ttgcgagcag
    11041 cttcttcaga acgacctagc ttagcagtcg ataccatttg catggcatta gtgatttgac
    11101 tcgtattttt tgttgaggcg atttttgttt taatatcatt tagagatact gccatctgac
    11161 acctctattc ttattggaag ctagattgat tgagaaactc tgtaatcgca gcatccaaga
    11221 ctgcttcttc tggcaagtct tttgtatcac gaatggtttc caaaatctct ggatgttgag
    11281 catcaaagaa ggcatggaac tcttcctcga aacgaacaat atcatctact ggaacagtat
    11341 ccaagaaacc atgtgtcaaa gcataaagaa tggttacttg tttctcaaca ggtaatggtt
    11401 tgtgaacagg ttgtttcaag acctcaacgg tacgacgtcc acggttcaac ttagcctgtg
    11461 ttgctgcgtc caagtcagaa ccaaacttag taaaggcttc caactcacgg tatgaagcaa
    11521 ggtcgatacg aagtgtacca gcaaccttct tcatggcttt gatttgtgca gaaccaccta
    11581 cacgagatac agatgaaccc gcatcgatgg ctggacgaat acctgcattg aaaaggccat
    11641 cgccaaggaa gatttgtcca tcagtgatag aaatcacgtt ggttgcgata taggctgaga
    11701 tatctcctgc ttgtgtctcg ataaatggta gggctgtaat tgatccacca ccaagttcat
    11761 cagaaacttt agctgagcgc tcaagcaaac ggctgtggag atagaaaaca tcccctggga
    11821 aggcttcacg acctggagga cgacgaagca agagcgacag ttcacgataa gctaccgctt
    11881 gttttgatag atcatcgtat acaatcaaaa catgcttacc ttgatacata aattcttccg
    11941 ccatagcaac cccagcataa ggagctagga agagcaatgg agatggttgt gaagcagagg
    12001 ctgtcacaac gattgtgtag tccaaggcac cgtactgacg aagtgtttct acttgcgtac
    12061 gaactgttga ttctttttgt ccaatcgcga cgtagataca gatcatatct tgatcttttt
    12121 ggttcaagat tgtatcaatc gcaatggttg ttttccctgt ctgacggtca ccgataatca
    12181 actcacgttg accacgacca atcggtacaa gggcgtcaat agctttcaaa ccagtttgca
    12241 atggttctga aacagactta cgttgcataa caccaggagc tggtgcttct actggacgag
    12301 ttttatcagt gtggatttct ccaagaccgt caactggacg accaagcgga tccacaacac
    12361 gaccaatcag actttcacct acagggactt ccatgatttt ccctgtacgg cggattgtat
    12421 cgccttcacg gatatctgta aagtcaccta ggatgataat accaacgtct gttgactcca
    12481 agttttgagc cataccataa gagccgtttt caaaattcaa caactctcca ctcatggcat
    12541 tttcaaggcc gtgagcacgc gcgataccat ccccgatata ggttacaaca cctgtttcag
    12601 tcacatcaaa attgggtttg aaattttcaa tttgttgctt aattaaagcg ctgatttctt
    12661 gtgcgttaat tgccaaaaga acaccacttt ctatttcaaa ttttctttaa caactttaag
    12721 ttgttgttta atactcacat caattgtctt gtgattggca aaaatgacaa aaccaccaat
    12781 gagactttca tcgatttgtt cttttacact ccttactttc agagacattt ttttctcaat
    12841 caaagggagc aaacgagtct tctgttcatc agttagagga taagcagacg taatcgtcac
    12901 ttcaaatcga tttgtttctt tttcaagtcg gttcaagcaa tctacaagca catcataaaa
    12961 aagatttgct ctgtgattgt aggccagaac ctggataaag ttttgtaata aaggtgacac
    13021 agaatcttgg aaaaaagcaa ttgttttttc cttatccgac tcgtctactg ccacttgttt
    13081 taaaaaagaa ggcagacctg ttttttcaac aacttgcttg atttgagtca agtctgaaaa
    13141 gatacggtct tcttctcctt tttcaagtac caattggaca aaaggcatgc tgtatttttc
    13201 aattaccttt actgttttct tgtccattaa gcttctccta gctgatcgat atactgatca
    13261 atgagtgctt tatgggcatg actgtcaagg ttttgtgaga tgattttacc agctaagctg
    13321 atggtcaaat ctgcgacctc acccttaaca ctctgtaaag cttctacttt attttgagca
    13381 atttcttgat tggctttttc ttttaagtgt cctgcttcta gtttagcatc tgctaagata
    13441 ttagccttac tttgctcagc tgtttccttt gcattttcaa tgattgtctt agcttcttta
    13501 cggctaccag ctaattcatc ttcgcgtttt tgagccaata cttctgcttt ttgacgggct
    13561 tcttcagctc tgtcaatatc tgaagcaatt ttttcagctc tttcttcgaa aatgcctgta
    13621 atattagacc atgcaaattt tttaatcaag actagcaaaa gaataaaaga gccagtaatt
    13681 aaaacaaaat taccaattaa ttcacctact gttacgtgca tcagttactc ctttctattc
    13741 ctcttcaata ttaatcttat tccctaaata cacagatgtc aaaagagtaa aaacataagc
    13801 ttggatgcag gaaataaaga cagaaaatgc agtccaagcc aaattagctc caaaggctac
    13861 tggataccaa taaatagctt ggtgggaaag aagaagtaac aaactcgtca tgacctctcc
    13921 tgcaaagata ttcccaaaaa tccgcaaagc caaagataag aagtttgtaa attcttccaa
    13981 gatattcatc ggtatgacaa aaacaggact cataaaagat tttatacttt ttttaaatcc
    14041 acgacgacga acgctttcta tatgtgtcaa caaaatgaca agaaaagata aggttaagtc
    14101 atactgtaaa ttagcggttg gcgaactcca ccagttagtc ccatcgatcg tttgaagctt
    14161 tgtcattaag ccaaggttat tggcaatcac catgaaaagg aataaacaaa ggaaaaagag
    14221 tgagtaatct ttcatgtagc gcgaaccaat gttaggttct gtaaatccaa taacaaaatc
    14281 atagacatac tcaagtacat tttgctttcc tttgggtttc aaggtcatat tgcggcttgc
    14341 ccaataaata aagacaaaaa taactcccac aatcaacaaa gtcatggcta acatagtcag
    14401 attgaagata acaggaccaa tagagatgat tggattaata ctttcttcca tctaaaatcc
    14461 tcccttttct tctcgttcca tgtatttatt tgataataaa tgagaagaca agagttacaa
    14521 agaaagttcc ttcaataaag gcaacaccta aaaacatcaa actacgaaac tcagaaagca
    14581 tatctggttg gcgtgctact gatttaaaca gtccattcat caataaacct tcaccgacag
    14641 atacgcccat acaggcaata cataagccta aaaatgttaa attcataaca aattctcctt
    14701 taaaataaaa tttacttact tagtgtagtc ctaaaattta aatttgtcaa cttttaaccg
    14761 ctattggaag cgtttaataa aaattatttc cattttgcta aatttcaagt tcaagttagc
    14821 catccagaag tcttctctgg gtgacttgta gtccaagcat tttttaggat agttgttaat
    14881 ccacttttcg atgaatgcga cttctttggg agtcattttc ttggttccct taggtaacca
    14941 tctacgaatg aacctgttgt gattctcatt agttcccctt tcccaagagg cataggggtg
    15001 cgcataataa atgtgctcct cagaaaatac atcagacaag cgattgaatt ccgttccatt
    15061 atctgccgtg atggaaagaa tcttgtgttg ttttaagatg agttttagag cctgattgac
    15121 cacctcagca cttttatttg gaatcaatcg gatgatctga tgtctactct ttcgatccgt
    15181 caagacaagc aagcagtagt ttttcgatct cgtaagtaga accgtatcaa tctcataatg
    15241 cccattctcc aagcgaagat tgatagcttc aggccgctgt gcgatggatt gaccagcagg
    15301 tttaaagttg gtgctagcct gtttcttaag cgcttttcct tttctagggt aaagcagatc
    15361 ctgcttgctt aaccccaatt ttccacgatg aatccaatag taaatggttg aaattcctac
    15421 gttaacccct ttagccatca ccatcatttc aggcgaaaat ttttggttat gatagtggag
    15481 aatcttttcc tttagttcct tggtcaagct tgatttcttg accgagcgct tgcgattgtt
    15541 ttcataagac tgttgagcat agtcggcaga ataaacctct ttgaagcacc cttttccaag
    15601 acattgtcgg actgtcccac gcttgatttc agtgtggata gtttgaggag cttttccaag
    15661 tagagaggca atttctctat ttgatttccc ttcttttttc catcgttcta ttaagcaacg
    15721 gctatcgatt gtcaaatgtt tgccttttgt agtataatag ttttgcatct ctgtgccttt
    15781 cttgtgtttg tggttgaaca acaagtatag cacagaggtg ttttcttatg cctacaagag
    15841 ctttcattat ttccactttc tttcggattt cactctattc tgaaaaactt gtgtatattt
    15901 actgaagcta gcaagtctta cctgtaaatt taatgaaagc aacacaaaat ccgagagggg
    15961 aatctcggat taatgtcaat ttaaaaagat agagagtttt tagtttaaat aaattgtttt
    16021 aaaatatcaa caacatcact tcttttctta acctgataag ccttaattcc taattttggg
    16081 gctacgatta tattgtcctc aatatcgtct agaaagacac aatttctagg ttataactgg
    16141 tatttatcaa tagttattca tatatatcag tccacctcca tactcacctt acagattctt
    16201 ttggtaataa tatctttgcc taatttagag atagtcttgc aaagaaaaaa cttccttgta
    16261 gccatgtttc tgataaaagt ccggtgcctg aaactggtaa gtattgacaa aggcaaaaca
    16321 acaatttcga ttcttagctt cactttctgc ctgttgcaat agttttgaac cgattccttg
    16381 ccctcgcagt tcctctttta caaacaaata ctcgatttct agccaatttc caaaagtctc
    16441 tgctatcaaa cctgccagga gattgccctt ttcatcttcg acataaagat taagtggctc
    16501 actttcagtc tcttctcttt ttgaacggtt ataaacacga agcagattcc ctatttcttg
    16561 cgatttatgt gattccttat tttccaatct aaagtatagt gaaatgaaat aaaacatgcg
    16621 caaatcgatt aaggaattta atctaatttc taacaatgtc ttagaaatca aagtgtacta
    16681 tgttaacttc aatgcactat acatctaata ctcaataaaa atcaaataat actcaataaa
    16741 aatcaaagag
//
LOCUS       DAVKDZ010000043        15122 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000043 DAVKDZ010000000
VERSION     DAVKDZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15122
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.044"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(126..416)
                     /locus_tag="VWZ30_001378"
     CDS             complement(126..416)
                     /locus_tag="VWZ30_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335101.1"
                     /translation="MLEQIIQSLFIIVAIGLILLVLYQIAKILGSLFVIGLLGLLAFM
                     EIYGIYLFFTERYLYVEDLAANGILSFTTFYIGFNVLLIFGLVAKVIRSRMA"
     gene            complement(431..730)
                     /locus_tag="VWZ30_001379"
     CDS             complement(431..730)
                     /locus_tag="VWZ30_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335102.1"
                     /translation="MTQTVEEQRYQLEEWLAKGFTSPEDRANYQALKEQYEDETLDYS
                     FSKREIIGQLDIIITTRENNFPDLDEVTKESYIDLIAQLDDLDKGQANYYRKQLV"
     gene            complement(800..7603)
                     /locus_tag="VWZ30_001380"
     CDS             complement(800..7603)
                     /locus_tag="VWZ30_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9335103.1"
                     /translation="MIQEQLLEMLRARLPRDQILLESFLRYQAVHFDEDWESLIQDFT
                     THRGVVPSPDQVVRFETEVSAFVKASPFDEATDLSSYTRTFGQAGLKKLPQLNSDEKA
                     LVIEVALFNLATRFQLLDQEGAYQSISVASLLEKGKAANLVNVYRVANNLSDRISRDI
                     EQFLLTYEPELDTRPIETISESDEIEVEPSPEITFREESFVIMASLEEDLSQLNLRTG
                     KTEHLPAYEHLNLSQKFEILSHFDQVRNSRPKLPNLRRGEFDHEMEMMPIYEEEELLI
                     YLEADGTIYDLQRPLTPQEEVILTEIGQTILAENTEKLTSLGIDLANFDENKQGVLID
                     ATGRFRLKNADLALLGGYPNASVTQLALTTELLQMGLTHDRAEFFLTSQLKLEDLRPI
                     AYAFLHEYLDIEEARTFERDKQSQPDVSFRDWRERLTQTESEIIMPQETPQNPIVEEA
                     LKRYPIDSTVSYKGQDLKVIAIEESGVNDLIRIELQNDFTDVIEQNPVLFLRTLEEIT
                     QALHLPSAEEKEEVKQPSQELDLFSFMLDDENQEDVFQEPVMEKEVEKSETLEEEIDS
                     ELFEVIEMTPTTDFHFPEDLTNFYPKTTRDKVETNVAAMRLVKALEAEERQATPDEQE
                     LLAKYVGWGGLANEFFDEYNPKFSKEREALKTLVTDKEYSDMKQSSLTAYYTDPALIR
                     QMWEKLEQDGFTGGKILDPSMGTGNFFAAMPKHLRENSELYGVELDTITGAIAKHLHP
                     NSHIEVKGFETVAFNENSFDLVISNVPFANIRIADSRYDKPYMIHDYFVKKSLDLVHD
                     GGQVAIISSTGTMDKRTENILQDIRETTDFLGGVRLPDTAFKAVAGTNVTTDMLFFQK
                     HVDKDYQAYDIAFSGAIRYDKDDRIWLNPYFDGEYNPQVLGTYEVRNFNGGTLSVKGN
                     PDNLIADVQIALKQVKAPRVVEPSDIFINSDVLARQVIDTSIPADIRESLEQYSFGYK
                     DSTVYYRDNKGIRVGTKTEEISYYVSEEGHFKAWDTKHSQKQIDRFNALEVTDSTALD
                     VYVTEEPAKRGRFKGYFKKTVFYEAPLSEKEVARIKGMVDIRNSYQEVIAIQRYYDYD
                     KDEFNHLLGKLNRRYDNFVKRFGYLNSPVNRNLFDSDDKYSLLASLEDEGLDPSGKTI
                     IYTKSLAFEKALVRPEKEVTEVSSALDALNSSLADGRGVDLDYMMSIYQTDSKASLIE
                     ELGDAIIPDPEPYLNDREVVYVSRQDFLLGDVVTKLEIVDLLIKEDNSDFPWVYYQGL
                     LEDVKPPRVTLADIDYRIGSRWIPLAVYGKFAQETFMGQTFDLTAPEVSTVLEVSPID
                     GTMSYQSKFAFRYSTATDRSLGVPGSRYDSGRKIFENLLNSNQPTITKQVEDGDKKKH
                     VTDVEKTTVLRAKETQLQELFQDFVASYPEVQQMIEETYNSFYNRTVSKVYDGSHLTI
                     DGLAQNISLRPHQKNAIQRIVEEKRALLAHEVGSGKTLTMLGAGFKLKELGMVHKPLY
                     VVPSSLTAQFGQEIMKFFPTKNVYVTTKKDFARAKRKQFVSRIITGDYDAIVIGDSQF
                     EKIPMSQEKQVAYIQDKLDQLREIKQGSDSDYTVKEAERSIKGLEHQLEELQKLERDT
                     FIEFENLGIDFLFVDEAHHFKNIRPITGLGNVAGITNTTSKKNVDMEMKVRQVQGEHD
                     YRNVVFATGTPVSNSISELYTMMSYIQPDVLERYQVSNFDSWVGAFGNIENSMELAPT
                     GDKYQPKKRFKKFVNLPELMRIYKETADIQTSDMLDLPVPEAKVIAVESELTEAQKYY
                     LEELVDRSDAIKSGSVDPSEDNMLKITGEARKLAIDMRLIDPAYTLSDNQKILQVVDN
                     VERIYREGELDKATQMIFSDIGTPKSKEEGFDVYNELKDLLVDRGIAKEAIAFVHDAN
                     TDEKKNSLSRKVNSGEVRILMASTEKGGTGLNVQSRMKAVHHLDVPWRPSDIVQRNGR
                     LIRQGNMHQEVDIYHYITKGSFDNYLWQTQENKLKYITQIMTSKDPVRSAEDIDEQTM
                     TASDFKALATGNPYLKLKMELENELTVLDNQKRAFHRSKDEYRHTISYCEQNLPALEK
                     RLSQYDLDIAQSLATKSQDFVMRFDNQMMDNRAEAGDYLRKLITYNRSETKEVRTLAN
                     FRGFELKMATRSPSEPLPDMVSLTISGSNQYSVSLDLKSDVGTIQRITNAIDHILEDQ
                     EKTEKMANNLKDKLAVARVEVEKVFPKEEDYQLVKAKYDILAPLVEQEAEVEEIDAAL
                     AKFNETTQPKKDLQLSLDI"
     gene            complement(7655..8206)
                     /locus_tag="VWZ30_001381"
     CDS             complement(7655..8206)
                     /locus_tag="VWZ30_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335104.1"
                     /translation="MAWNYDTISKTLSEMAQENYEDMVKALLTMELSIKSKSLLDRLY
                     QDFMGIDDLSLVSDDLRLRADGYQEQLQEELTDLLDKLYRTGEGASFIMEVIASNNIF
                     DTLVQYEVLDEDDYSSLTLETLQDIIQKDLPLTSQDYFGDVTYLALQKDLLDQKSHFL
                     QQYVTTLVDKLPQEKDQSTLVLD"
     gene            complement(8190..8381)
                     /locus_tag="VWZ30_001382"
     CDS             complement(8190..8381)
                     /locus_tag="VWZ30_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEU9335105.1"
                     /translation="MEPKSIYTMDSDQDGLTDAQELALGTNLFSSDTDSDGLTDLEEV
                     QQGLNPIQQGKESSYGLEL"
     gene            complement(8382..13286)
                     /locus_tag="VWZ30_001383"
     CDS             complement(8382..13286)
                     /locus_tag="VWZ30_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9335106.1"
                     /translation="MTKQCHHHFLVNQEQAEKHVFRKSKKYRTLCSVALGTMVTAVVA
                     WGGQVAQADEVTMPPLDKTVQLTENNATNLPEAQPAPVAEQTDSLASTGQSDGTITVT
                     VPHDTVTNAINQATAEGVTTVQDQPMDLGNTTSASDTSKQLDKAEADAAKQAEDITRV
                     TNTYKADKAAYEQDKTRVEKGNAALVASHKEATQAGKALNSSVDTTASEVQTQDKSAN
                     VTITTQTVPSGEGSTVSGYQDYTSAVAAIDKQNKASLADYITKKQAADAITAKNLAVQ
                     KENEAGLANAKAENEAITKRNQAEQKAIDDENKAGQAAVDTYNKNQQKLVTDREAEIA
                     AIIKRNKEKEEAAKKENEAIDAYNAKEMNRYKRDLADISKGEEGYISQALAQALNLNN
                     GEPQARHSADTRNPNRIIAKGDAMLGGYSKILDSTGFFVYDTFKTGETLSFTYQNLQN
                     VSFDGKKITKVAYDITNLVSPTETDSVQLVVPNDPTEGFIAYRNDGTGNWRTDKMEFR
                     VKAKYFLEDGSQVSFSKEKPGVFTHSSLNHNDIGLEYVKDTSGKFVPINGSTIQVTDE
                     GLARSLSSNRASDLNLPEEWDTSFSKYAYKGAIVSTVTSGNTYTVTFGQGDMPQNAGL
                     TYWFALNTLPVARTVKPYSPKPHVSPKLDPVPEPIKVVPKTFTPKTFTPEPPVIFKEK
                     PLEKVTQPRLTLTKVTFAKEPRSEPLPKAPQVPTVHYHDYRLTTTPEIMKKVVNTDQD
                     NLHDKTIAKDSTVIYPLTVDVFSSNRAKTTTLTFEDYLPAGYAFDKEKTQAENGNYTL
                     TFDEAKNFVTLTAKEALLQEVNQDLTKSYQLVAPKLYGSLQNDGATYSNSYKLLINKG
                     TSNAYTVTSNVVTVRTPGDGKITSRITPQKRNENEDGVVINDTVVALGTTNHYRLTWD
                     LDQYKGDTSSKETIARGFFFVDDYPEEVLDLVDKGTSITTLDGKAVSGITVKAYASLS
                     EAPKDLQDKLAHAKISPKGAFRIFQPNDNQAFYDQYVKTGTSLNLLTKMTVKDSLYGQ
                     TKTYRNKAYQVDFGNGYKTNEVTNTLVSPTPKKQNLNKDKVDINGKPMVVGSQNYYTL
                     SWDLDQYRGIKADKAQIAKGFYFVDDYPEEAVLPDDTAIQLTTSNGKAVIGVTVKNYT
                     SLSEAPKPLQASFEKRKIAPKGAFQVFMAEDPQAFYDSYVTKGQNITIVTPMTVREEM
                     LNSGKSYDNVAYQVDFGQAYETNTVTNHVPKVNPHKTNTNKEGVSIDGKTVLPNTVNY
                     YKIVLDYSQYKDLVVTEDTLAKGFYMVDDYPEEALTLNADGVQVMDKAGNLVKGISVK
                     AYASISEAPQVVQEAMAKRQLTPKGAIQVLSADDPKAFYETYVKTGQTLVVTLPMTIK
                     NELTKTGGKYENTAYQIDFSLAYVTETVVNNVPKLDPQKDVVIDLSQKENSLDGKEVA
                     LNQVFNYRLVGALIPGNRATPLIEYRFDDDYDESHDDYNGVYKAYTVVDVTLKDGTVL
                     LKGTEVTKYTLQHVDTSQGTVTISFDKEFLEKLAEESEFQADVYLQIKRIASGEVENT
                     VLHTVNGYTISSNTVKTTTPEPEPPTPNQPTPPQPLIPTQEPPVPASVLPNTGESQSL
                     LALVGGGLLLGLAYVLAKRKMEDN"
     gene            13480..14070
                     /locus_tag="VWZ30_001384"
     CDS             13480..14070
                     /locus_tag="VWZ30_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEU9335107.1"
                     /translation="MSKKELLLEYINNHNGLITHRDCKKLGIPTIYLTRLEDEGVIFR
                     VEKGIYLSSSGDYDEYYFFQYRYPRTVFSYISSLYLQGLTDEIPQYFEVTVPKDYRFR
                     NRPLNLNSYTVSKDIINMGVTSVKTPMGNTVNVYNFERVLCDFILNRSNIDNELFVKT
                     VQTYSNFPEKNLAKLYDYAQKLNILDDVKRTLEVLL"
     gene            14067..14912
                     /locus_tag="VWZ30_001385"
     CDS             14067..14912
                     /locus_tag="VWZ30_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEU9335108.1"
                     /translation="MNKAKLTALCHNISKQTGLTFNSVMTYYFLEVILKKLSRSSYAD
                     HYIFKGGFLLSNIIGVESRSTVDIDFLFHKQTLSEENVQQQLEEILSEVKNDIQFSIQ
                     SITTIKESDDYGGYRATILCQLENIKQIIHLDIATGDVVTPQPITYDYKAIFNDENFP
                     IIAYTIETVLAEKLQTIYSRSFLNSRSKDFYDIYILSKLKKDDIDLAQLKMACEKTFS
                     YRETELNFNNIIQLLETFKSDSTQQQQWQNYSKKYSYTKGISFSEILDEMIRLVSLLI
                     SLNNE"
BASE COUNT         4095 a         3211 c         2976 g         4840 t
ORIGIN      
        1 ataggaaatc gaacgacgga gcgactactc ctagggagat ttatcttttt ccacagagtt
       61 gtaggcaagt tcagttttca agatacaaat cattagaaga gtctgggaca atagtctcca
      121 ttattttatg ccatccgact tctaataacc tttgccacaa gtccgaatat aagtagcaca
      181 ttaaatccaa tatagaaagt ggtaaaactc aaaataccat tggccgctaa atcttcgaca
      241 taaaggtatc gctctgtgaa aaagagataa atgccataga tttccataaa ggctaggagg
      301 ccaagcagtc cgataacaaa cagacttcct aatattttcg caatctgata gaggacgagc
      361 aaaatcagcc ctattgctac gataatgaaa agactttgaa tgatttgttc taacatatct
      421 acgcctcttt ctacactaat tgcttacggt aatagttagc ctgcccctta tctaggtcat
      481 caagttgagc aattaagtca atatagcttt ctttggtcac ctcatctaag tctggaaaat
      541 tattttcacg agtggtaata atgatatcta attgaccaat gatttctcgc tttgaaaagc
      601 tgtaatcaag ggtttcatct tcatattgtt cctttaaggc ttggtagtta gctcggtctt
      661 cagggcttgt aaaacctttt gctagccatt cttctagttg gtatctttgt tcttctactg
      721 tttgggtcat gttctgttct cctttggtga tttcttaatg tcattttacc gcgttcaaaa
      781 ctatttgtca gcctttttat taaatatcaa gagaaagttg aaggtctttt ttcggttggg
      841 tcgtttcatt aaatttagct agtgctgcat caatttcctc aacctctgct tcttgttcta
      901 ccaagggagc taatatgtca tacttagctt taaccagctg ataatcctct tctttaggaa
      961 aaaccttctc tacttcaacc cttgcgactg ctagcttatc ttttagatta ttggccattt
     1021 tttctgtttt ttcttggtcc tctaggatat ggtcaatggc attagtaatg cgttggatgg
     1081 ttcccacatc tgatttcaag tctaaagaga cagaatactg gttgctacct gaaatggtta
     1141 aagacaccat atctggcaac ggctcactag gacttctggt tgccatttta agctcaaatc
     1201 ctctaaaatt agccagtgtt ctcacttctt tggtttcaga acggttatag gtaatgagtt
     1261 ttcgtagata gtctccagct tctgcacggt tatccatcat ttgattgtca aaccgcatga
     1321 caaaatcttg tgacttagtt gccaaagatt gagcaatatc aaggtcatat tgactgaggc
     1381 gtttctcaag ggcagggagg ttctgctcac agtaagaaat ggtatggcga tactcatctt
     1441 tggagcgatg aaaggcacgc ttttgattgt ccaataccgt caactcattc tctagttcca
     1501 tcttgagttt cagataagga ttacctgtcg ctaaagcctt aaagtctgaa gcggtcatgg
     1561 tttgttcatc aatgtcttct gccgaccgta ctgggtcttt agaggtcatg atttgcgtga
     1621 tgtatttgag tttattctcc tgagtttgcc agaggtagtt atcaaatgac cctttggtaa
     1681 tataatgata gatatctacc tcttggtgca tattgccttg acgaattaac ctaccattgc
     1741 gttggacaat atcagaggga cgccagggaa catctaaatg gtggactgct ttcatacgtg
     1801 actgtacatt taatcctgtt ccaccttttt ccgtagaagc catgagaatc ctgacttccc
     1861 cactattgac cttgcgtgag agagagtttt tcttctcatc cgtattagcg tcatgaacaa
     1921 aggcaattgc ttctttggca atccctctat ccactaagag gtctttcaat tcattgtaaa
     1981 catcaaaccc ttcttcttta cttttaggcg ttccaatatc agagaaaatc atctgggtag
     2041 ccttatcaag ctcaccctca cggtaaatgc gttccacatt gtccaccact tgaagaatct
     2101 tctgattgtc cgataaggta taggctgggt caatgagacg catgtcaata gctagttttc
     2161 tagcttcccc agttatcttt aacatattat cctcactagg gtcaacgcta cctgacttaa
     2221 tggcgtctga acggtcaacc aactcttcaa ggtagtattt ctgagcttct gtgagttcac
     2281 tctccaccgc aatgactttg gcttcaggta caggtaaatc aagcatatct gaggtctgaa
     2341 tatcagctgt ttccttataa atccgcatga gttcaggcag gttcacaaac ttcttaaagc
     2401 gtttcttagg ttggtactta tctcctgttg gggcaagttc catagaattc tcaatattcc
     2461 caaaagctcc cacccatgag tcaaagtttg agacttgata gcgttctaag acatctggtt
     2521 ggatataact catcatggta tagagttcac taatagagtt agagactggt gttcctgtcg
     2581 caaagaccac attacgatag tcgtgttctc cttgaacctg tctcactttc atttccatat
     2641 ccacattctt tttagaggtc gtgttggtaa ttcctgcgac attaccaagt ccagtaatgg
     2701 ggcgaatatt cttaaaatga tgagcttcgt ccacaaagag aaagtcaatc ccaaggtttt
     2761 caaactcaat aaaggtatct cgctccagtt tttggagttc ttccaattgg tgctccaacc
     2821 ccttgataga acgctctgct tctttaaccg tatagtcact atcactgcct tgtttaatct
     2881 ctcgtaattg gtccagctta tcttggatat aggcgacctg cttttcctga ctcatgggga
     2941 ttttctcaaa ctgggaatcc ccaatgacaa tggcatcgta gtcccctgtg atgatccgtg
     3001 agacaaactg cttacgtttg gctctcgcaa aatctttctt ggtcgtcacg taaacattct
     3061 tggttgggaa gaacttcatg atttcttggc caaactgagc ggtcagactt gatggcacca
     3121 cataaagagg cttatgcacc atacccaatt ctttcagttt gaatcctgcc ccaagcatgg
     3181 ttaaggtctt acctgaaccc acttcatgag ctagaagcgc tcgtttttct tccacaatgc
     3241 gttgaatagc attcttctgg tgagggcgaa gagaaatgtt ttgtgctagc ccatcaatgg
     3301 ttaaatggct gccatcataa acttttgaga ctgtacgatt ataaaagcta ttataggtct
     3361 cttcaatcat ctgttgcacc tctgggtaac tagccacaaa atcttggaag agttcttgaa
     3421 gttgtgtttc cttagcacga agaaccgttg tcttctccac gtccgtcaca tgctttttct
     3481 tatccccatc ttccacttgt ttggtaatgg ttggttgatt ggaattaagc aggttttcaa
     3541 agattttgcg accactatca tagcgtgacc caggaacacc taaacttcta tccgttgcgg
     3601 ttgaatatct aaaggcaaac ttagattggt aggacattgt gccatcaatc ggactaactt
     3661 caagaacagt tgatacctct ggggcggtca ggtcaaaagt ttgtcccata aaggtttctt
     3721 gggcaaattt cccataaacg gctagaggaa tccatcgtga gccaatccga taatcaatat
     3781 cagctaaagt cacacgtggt ggtttcacgt cttctaagag accttgataa tagacccaag
     3841 gaaaatcaga attgtcttct ttaatgagta agtccactat ctctagctta gtcactacat
     3901 ctcctaataa gaaatcctgc cgcgacacat ataccacttc cctatcgttt agatagggtt
     3961 ctgggtctgg aataatagcg tctcccaatt cctcaatcaa agacgctttg gaatccgtct
     4021 gataaataga catcatataa tccaaatcca ccccacgacc atctgcgagg ctggaattga
     4081 gagcatcaag ggcagaagac acctctgtta cttccttttc gggacgaacc aaggcttttt
     4141 caaaggcaag tgacttggtg taaatgatag tcttaccact tgggtcaagt ccttcatctt
     4201 ctagactagc aaggagcgaa tacttatcgt cactatcaaa tagattacgg ttcacaggac
     4261 tattcaaata tccaaatcgt ttaacaaagt tatcatatct gcgattgagt tttcctagca
     4321 agtgattaaa ctcgtctttg tcgtaatcat agtagcgttg aatggcaatc acctcttggt
     4381 agctatttcg aatatcgacc atccctttaa ttcgtgccac ttctttctca gacaaaggag
     4441 cctcataaaa gacggtcttt ttgaaatacc ccttaaatcg gccacgcttt gcaggttctt
     4501 ctgtcacata aacatcaaga gctgtgctat cagtcacctc taaggcatta aagcggtcaa
     4561 tctgcttttg agaatgcttg gtatcccatg ccttaaagtg accctcctcg ctcacatagt
     4621 agctgatttc ttcagtctta gtccctacac ggattccctt gttatctcga tagtaaactg
     4681 tggaatcctt ataaccaaaa ctatactgtt ctaaactctc tctgatatca gctggaatcg
     4741 aggtatcaat gacttgtctt gccagtacgt ctgaattgat gaaaatgtca gaaggttcaa
     4801 caactctagg cgctttaacc tgtttaagag caatctgaac atctgcaatc aagttatcag
     4861 gattgccttt aacagaaagg gtgcctccat taaagtttct cacctcataa gttccaagaa
     4921 cttgaggatt gtactccccg tcaaagtaag gattcagcca aatacggtca tccttgtcat
     4981 aacgaatagc acctgaaaag gcaatgtcat aagcttgata atccttgtcc acatgtttct
     5041 ggaagaacaa catatccgtt gtgacattgg tacctgcaac ggccttaaaa gccgtatctg
     5101 gcaaacgcac accaccaaga aaatcagttg tttcgcgaat gtcctggagg atattttctg
     5161 tccgtttgtc catggttcca gtggaggaaa tgatagccac ttgaccacca tcatgcacca
     5221 aatcaagcga ttttttaaca aaataatcat gaatcatata gggcttatca taacgactat
     5281 cggcaatcct gatattggca aacggcacat ttgagatgac caaatcaaag ctattctcat
     5341 taaaggcaac ggtctcaaag cccttcactt caatgtgact atttggatgg aggtgcttgg
     5401 caatagctcc tgtaatcgtg tctaattcca caccatacaa ctcactattt tctctcaggt
     5461 gtttaggcat agccgcaaag aagtttcctg tgcccatcga agggtctagg attttacctc
     5521 ctgtaaagcc atcctgctcc aacttctccc acatctgacg gataagagca gggtctgtgt
     5581 aataggcagt caaagaggat tgtttcatgt ccgaatactc tttatctgtg actagcgttt
     5641 ttaatgcctc acgttcctta gaaaatttag gattatactc gtcaaagaac tcattagcaa
     5701 gccctcccca gcccacatat ttagcaagga gttcttgttc atcaggcgtg gcttgtcgtt
     5761 cttcagcttc aagagctttt acaagacgca tggcagccac gttcgtttca accttatctc
     5821 ttgttgtctt agggtaaaag tttgtcagat cttctgggaa gtgaaaatca gtggttggtg
     5881 tcatctcaat gacttcaaaa agctctgaat caatctcttc ttcaagagtt tcagattttt
     5941 ccacttcttt ttccatgact ggttcttgaa aaacgtcttc ttggttctca tcgtctagca
     6001 taaaggaaaa gaggtctaat tcttggctag gttgtttcac ttcttctttt tcctcggcag
     6061 aaggaagatg gagagcctga gtgatctcct ctaatgtccg taagaaaaga actggatttt
     6121 gttcaatcac atctgtaaaa tcattttgta gttcaatccg aatcaggtca ttgacacctg
     6181 actcttctat ggcaatcacc tttaagtcct gccccttata ggacaccgtg gaatcaatcg
     6241 gataacgttt aagggcttcc tctacaatag gattttgagg tgtttcttga ggcatgataa
     6301 tttcagattc tgtttgtgtt aggcgctctc gccagtctct aaaggatacg tctggctggg
     6361 actgtttgtc cctttcaaag gttctagctt cttcaatatc gaggtattca tgtaagaaag
     6421 cataggcaat cggtcttaag tcttctaact ttaactgact ggtcaagaag aactctgccc
     6481 tgtcatgcgt tagtcccatc tgaagcaatt ctgttgtaag agctaattga gtgacggatg
     6541 cgttaggata gccccctaat aaggctaagt ctgcattctt caaacggaaa cgccctgtgg
     6601 catctatcag taccccttgt ttattctcat caaagttagc taagtctatc cctaaactgg
     6661 tcagtttctc agtattctct gctaaaatcg tttgaccaat ttcagttaag atcacttctt
     6721 cttgcggtgt taggggacgt tgtaagtcgt aaatcgtccc atctgcttct aaatagatta
     6781 atagctcttc ttcctcatag ataggcatca tttccatctc atgatcaaac tctccacgtc
     6841 ttagatttgg cagttttgga cgactatttc tcacttggtc aaaatgactc aatatctcaa
     6901 acttctgaga caagtttaga tgttcatagg ctggtaaatg ttctgttttc cctgttcgca
     6961 aattgagttg cgagaggtct tcttcaagac tcgccatgat gacaaaactc tcttcacgaa
     7021 aggtaatttc tggactgggt tctacttcta tttcgtcact ctcggagata gtctcaatag
     7081 gtctggtatc cagctccggt tcataagtta agagaaattg ttcaatgtca cggctaatac
     7141 gatcagataa gttgttagcg actcggtaga cgttgaccaa gttagcagct ttgccttttt
     7201 ccaaaagaga agctaccgaa atagactgat aagctccctc ttggtctaat agctgaaagc
     7261 gagtagctag gttaaagagg gctacctcaa tcaccaaggc tttctcatcg ctgtttaact
     7321 gaggtaactt ctttaggcct gcttggccaa aggtccgcgt atagctactg aggtctgtcg
     7381 cttcgtcaaa agggctcgcc ttcacaaaag cagaaacttc tgtttcaaag cgaaccactt
     7441 gatcaggtga ggggactact cctctatggg ttgtaaaatc ttggataagg ctctcccaat
     7501 cttcatcaaa atgaactgct tgatagcgta aaaaggattc taaaagaatc tggtcacgtg
     7561 gcaatcttgc ccgcaacatc tcgagtaact gctcttgaat catacgttcc tccaaatcaa
     7621 aaaaggagct gagaggtagt ctcagcccac tgctttagtc taggactaag gtgctttggt
     7681 ctttttcttg tggcaactta tccactaaag tcgtcacata ctgttgtaag aaatggcttt
     7741 tctggtctaa caaatctttc tgcaaggcaa gataggtcac atctccaaaa taatcttgac
     7801 tagtgagagg caagtccttt tggataatat cttgcaaggt ttctaaggtg agcgatgaat
     7861 aatcgtcctc atccaaaacc tcatattgaa caagggtatc aaaaatatta ttagacgcta
     7921 tcacttccat aatgaaacta gctccctctc ctgttcgata gagtttatct aacaaatccg
     7981 tgagttcttc ttgaagctgt tcctgataac cgtctgctct taggcgcagg tcatcactga
     8041 ctaaagacaa gtcatcaatg cccataaagt cttgatagag cctgtcaaga agtgacttgc
     8101 ttttgatact caattccatg gttaataagg ctttgaccat atcttcatag ttttcttgtg
     8161 ccatctcaga gagagttttt gaaatcgtgt cataattcca agccataact gctctcctta
     8221 ccttgttgaa ttggattcaa gccttgttgg acttcttcaa ggtctgtcag tccatcacta
     8281 tccgtatcac tgctaaaaag attggtccct agagctaact cctgagcatc cgttaagcca
     8341 tcttggtctg aatccattgt gtaaatgctt ttaggttcca tttaattgtc ctccattttg
     8401 cgtttagcaa ggacgtaggc taagcctaaa agaagtcctc caccgacaag cgccaaaaga
     8461 gattgactct ctcctgtatt tggtaagaca cttgctggaa ctggtggttc ttgtgttgga
     8521 ataagtggtt ggggtggtgt tggttgattc ggagttggtg gctctggttc aggagtagtt
     8581 gttttaaccg tgtttgaact gatggtgtag ccattgacgg tatgtagtac cgtattttct
     8641 acttcacctg aagcgattcg ttttatctgc aagtaaacat ctgcctgaaa ctcggattct
     8701 tctgctagtt tttctaggaa ttccttgtca aaactgatgg taaccgttcc ttgtgacgtg
     8761 tcgacatgtt gtagcgtgta cttagtcact tctgtccctt ttagtaaaac cgtcccatct
     8821 tttagagtga catctaccac agtataagcc ttgtaaacac cattatagtc gtcatggctt
     8881 tcatcgtaat catcgtcaaa gcggtattcg atgagtggtg tcgcacgatt accaggaata
     8941 agtgctccca ccaagcggta gttaaagacc tgattcaagg caacttcttt tccatcaaga
     9001 ctgttctcct tttgtgacaa atcaatcacc acatcttttt gtgggtctag ttttggaaca
     9061 ttattgacca ctgtttctgt cacataagcc aaactaaagt caatctgata agccgtgttt
     9121 tcatacttgc caccagtctt tgtcaactca ttcttaatgg tcatcggaag cgtcaccacc
     9181 aaggtttgac ctgtcttaac ataggtctcg taaaaagctt ttggatcatc agcacttaaa
     9241 acttgaatgg ctccttttgg tgtaagttgg cgtttggcca tagcttcttg caccacttga
     9301 ggcgcttctg atatcgaagc ataggctttg acagaaatcc ctttgacaag atttcccgcc
     9361 ttatccatca cttgaacacc gtctgcattt agtgttagag cttcttctgg atagtcatct
     9421 accatgtaaa aacctttggc aagggtatcc tccgtcacta ccaagtcctt gtactgacta
     9481 taatccagaa caatcttata gtaatttacg gtattaggaa gaacggtttt gccatcaata
     9541 gacactccct ctttattggt attggtctta tgaggattta cctttggcac gtgattggtc
     9601 accgtattgg tttcataggc ttgcccaaag tctacttggt aagccacgtt atcataagac
     9661 ttccctgaat taagcatctc ctcacgaact gtcatcggtg taacgatggt aatgttttgg
     9721 cctttggtca cataagaatc ataaaaggct tgtggatctt ctgccataaa gacttggaaa
     9781 gctcctttag gcgcaatctt gcgtttctca aaggatgctt gtaggggttt aggggcttct
     9841 gataaactcg tgtagttttt taccgtaaca cctatgacag ccttgccgtt agatgtcgtt
     9901 agttgaatag ctgtgtcatc tggtagcaca gcttcttcag gatagtcatc cacaaagtag
     9961 aagccttttg cgatttgagc tttatcagct ttaatgccac ggtattggtc taaatcccat
    10021 gacaaggtat agtagttttg tgaacccact accatcggtt ttccattgat gtccacttta
    10081 tccttattaa ggttttgttt cttaggtgtg gggctgacaa gggtattggt cacctcattt
    10141 gttttatagc cattcccaaa atcaacttgg taggctttgt ttcgataagt cttagtttga
    10201 ccataaaggc tgtctttgac ggtcattttg gtgagaaggt tcaaagaggt tcctgtttta
    10261 acgtactggt catagaaagc ctgattgtcg ttaggctgga agattcggaa agctccttta
    10321 ggagaaatct tggcatgagc gagtttatct tgaaggtctt taggagcttc tgacagcgac
    10381 gcataggcct taacagtaat ccctgatacg gctttaccat caagagtggt gatacttgtt
    10441 cctttatcca ccaaatccaa gacttcttct gggtaatcat ccacaaagaa gaaaccacga
    10501 gcaatcgttt ctttagaaga ggtatcacct ttatattgat ctaaatccca cgtcaaacgg
    10561 taatggttag tcgttcctaa agccaccacc gtatcgttaa tgaccacacc gtcttcattc
    10621 tcattgcgtt tttgaggagt aatacggcta gtgattttcc catcaccagg tgtacgaacg
    10681 gtcaccacgt tagaggtcac tgtataggca tttgacgttc ccttattgat gaggagctta
    10741 taactattgg aataggtagc cccatcattt tgaaggctac cataaagttt aggagccacc
    10801 agttgataag acttagtgag gtcttgattg acctcttgaa gcaaggcttc tttggcagtc
    10861 agggtcacaa agttcttagc ttcatcaaag gtaagcgtat aattcccatt ttctgcttgt
    10921 gttttttctt tatcaaaggc atagcctgct ggaaggtaat cttcaaacgt aagggtagtt
    10981 gttttggcac gatttgaaga aaaaacgtca actgttaaag gataaataac tgtcgaatcc
    11041 ttggcgatag ttttgtcatg aagattgtct tggtcggtat tgaccacttt tttcatgatt
    11101 tcaggggtcg ttgtcagacg atagtcgtga taatgaaccg ttggtacttg tggtgcttta
    11161 ggcaaaggtt cagacctagg ttctttagca aaggtcacct ttgtcaaagt caagcgaggt
    11221 tgagtcactt tctccagtgg tttttcctta aagatcacag gtggctctgg ggtaaaggtc
    11281 tttggggtaa aagtttttgg cacgacttta atcggctctg ggactgggtc tagttttggg
    11341 ctcacatgtg gtttcggact ataaggtttg acggttcgtg caacaggtaa ggtgtttaag
    11401 gcaaaccaat aggtcagtcc tgcattctgg ggcatatcgc cttgtccaaa agtcacggtg
    11461 taggtattgc ctgatgtgac ggttgagaca atcgctccct tgtaagcata cttgctaaaa
    11521 gaggtatccc attcttctgg gaggttcaaa tcactagcac ggtttgaaga aagtgaacgt
    11581 gctaggcctt catctgtcac ttgaatggtt gagccattga tagggacaaa cttgcctgat
    11641 gtgtctttga catactctaa gccaatgtca ttatgattaa gtgaggaatg ggtaaagaca
    11701 cctggttttt ccttgctaaa gctgacttgc gagccatcct ctaagaaata cttggctttg
    11761 acacgaaact ccattttatc ggttcgccaa ttccctgtcc catcattacg ataggcaata
    11821 aagccttctg ttgggtcatt aggcacaact aattgtacag aatccgtctc agttggagac
    11881 actaggtttg tgatgtcata agcgactttg gtaatctttt taccatcaaa actcacattt
    11941 tgaaggtttt gataagtgaa tgaaagggtt tctccagttt taaaggtatc atagacgaag
    12001 aaaccagtag aatctaggat tttagaatac ccaccaagca tggcgtcacc cttagcaatg
    12061 atgcgattag gatttctcgt gtcagctgaa tgtcgggctt gcggttcccc attgttcaag
    12121 ttgagagcct gagcaagagc ttgtgagatg taaccttcct ctccttttga gatgtcagct
    12181 aagtcacgtt tgtatcggtt catttctttg gcattgtagg catcaatggc ttcattttct
    12241 ttcttagcag cttcttcttt ctccttattg cgttttataa tagcagcaat ctcagcttca
    12301 cggtctgtca ccaatttttg ttggttctta ttgtaggtat ccacagcagc ttgacctgct
    12361 ttattttcat catcaatcgc tttttgttct gcttgattgc gtttagtaat cgcttcattc
    12421 tctgcctttg cattcgcaag acctgcttca ttttcctttt gaacagctag gttctttgcg
    12481 gtaatggcat ctgcggcttg ttttttcgtg atatagtccg caagactagc cttgttttgt
    12541 ttatcaatag cagctaccgc agatgtgtag tcctgataac ctgaaacagt tgatccctct
    12601 cctgacggaa cggtttgagt tgtaatagtc acgttcgcag acttgtcctg agtctgcact
    12661 tctgaagccg tggtatctac tgaactattc aaggcctttc ctgcttgagt agcttcttta
    12721 tgactagcaa ccaacgcagc attccctttt tcgacacgag ttttatcttg ttcataagcg
    12781 gctttgtcag ctttataggt atttgtcacc cgagtgatgt cctcagcttg tttggcagca
    12841 tccgcttccg ctttatctaa ttgcttactg gtatcactcg cagaggttgt gtttcctaaa
    12901 tccatcggct ggtcttgaac agtagttacc ccttcagcag ttgcttgatt gatggcattg
    12961 gttaccgtat catgaggaac cgttactgtg atagtcccat ctgattgacc ggttgacgca
    13021 aggctatcag tctgttcagc aactggtgct ggctgagctt ctggtaaatt ggtcgcatta
    13081 ttttccgtta actgaacagt cttatctagt ggtggcattg tcacttcgtc agcctgggct
    13141 acttgcccac cccaagcgac aacagctgtc accatagttc caagtgctac cgaacacagc
    13201 gtacggtact tcttactttt acggaagaca tgcttttctg cttgttcctg attgactaaa
    13261 aagtgatgat gacattgttt ggtcataaaa agtccttctt ttcttttgat aagtagcaac
    13321 aatctgtcac tctctcctca tcataaaaaa gacgacttga ttttggtatc acatcacata
    13381 agagactagt aaatgctaat taataacacc caaaatgttg cttctgcacc gttaaaatgc
    13441 taaaattaaa gtacaaaagc aacaaagaaa ggagagatta tgtctaaaaa agaattgtta
    13501 cttgagtaca taaacaatca caatggtctg attacccata gagattgtaa aaaattaggt
    13561 attccaacca tctatctaac gagactagaa gacgaaggag ttatctttag ggtcgaaaaa
    13621 ggtatctatc tatcttctag tggagattat gatgaatact atttcttcca atatcgttat
    13681 cctagaactg tcttttctta tatctcttct ctttatctac agggacttac tgatgaaatt
    13741 cctcaatatt ttgaagtaac agttcccaag gactatcgtt ttagaaacag accattgaat
    13801 ctaaatagct acactgtttc taaagatatt attaacatgg gggttaccag tgtcaaaaca
    13861 cctatgggaa atacggttaa cgtttataat tttgaacgtg tactatgcga ttttatcctt
    13921 aatcgaagta acattgataa tgagctcttt gttaaaacag ttcaaactta tagtaacttc
    13981 cctgaaaaaa atcttgcaaa actttatgac tatgctcaaa aattaaatat cttagatgat
    14041 gttaaacgaa ccttggaggt actcttatga ataaagcaaa attaacagca ctctgccata
    14101 acatttctaa acagaccggc ctaactttta actcagtaat gacctactac tttcttgaag
    14161 tcattttgaa aaagctaagt cgaagttctt atgcagacca ttatattttt aagggaggat
    14221 ttttgctttc aaatatcata ggagttgaat ctcgtagcac ggtggatatt gattttctct
    14281 ttcataaaca aacactatca gaagaaaatg ttcaacaaca actggaagaa attttaagtg
    14341 aagtaaaaaa cgatattcaa ttttcaattc aatccattac aacgattaaa gaaagcgatg
    14401 attacggtgg ttaccgagca accattttgt gtcaactgga aaacattaaa caaattatcc
    14461 atttagatat tgcgactgga gatgttgtta cacctcagcc cattacctac gattacaaag
    14521 ctatttttaa tgatgagaat ttccccatca ttgcttatac aattgaaacg gtgctagctg
    14581 aaaaattaca aaccatttac tcaaggagct tcttaaacag tcgtagcaaa gatttttacg
    14641 atatctatat cctttcaaag ttaaagaaag acgacattga tttggcacaa ttaaagatgg
    14701 cttgtgaaaa aaccttctct tatcgtgaaa ctgaactcaa ttttaataac atcattcaac
    14761 tgcttgaaac cttcaaatct gactctactc agcagcaaca atggcaaaat tattcaaaaa
    14821 aatatagtta tacaaaaggg atttcctttt cagaaatcct tgatgagatg attcgtttag
    14881 tcagtctttt aatctcttta aacaatgaat aaaacaactt ttcggttatc ttttaaccga
    14941 tacagacaac attaaaagga tatgcgagca actccttacc ttgttcgcat atcctttttc
    15001 ttatttcatc gtatacgcaa aactaatcgt accatctact cctgataact tacggaaggt
    15061 gtaattggga ttaccattgt agttggtctc tgagatgagg aacgatccat ctgggtaaac
    15121 ct
//
LOCUS       DAVKDZ010000044        14899 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000044 DAVKDZ010000000
VERSION     DAVKDZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14899
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.045"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            395..4006
                     /locus_tag="VWZ30_001386"
     CDS             395..4006
                     /locus_tag="VWZ30_001386"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9335109.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHA
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVVMSERLVKDDIYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEDAEKATKAEATEEAAEQE"
     gene            4040..7717
                     /gene="rpoC"
                     /locus_tag="VWZ30_001387"
     CDS             4040..7717
                     /gene="rpoC"
                     /locus_tag="VWZ30_001387"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU9335110.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKVL
                     FNDIMPEGLPYLQEPNNGNLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYQHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIADQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            7818..8231
                     /gene="ndk"
                     /locus_tag="VWZ30_001388"
     CDS             7818..8231
                     /gene="ndk"
                     /locus_tag="VWZ30_001388"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU9335111.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGTKVIEIWRTMMGATRPEEALPGTI
                     RGDFAKAAEENEVIQNVVHGSDSEESAKREIALWF"
     gene            8465..8688
                     /gene="tnpA"
                     /locus_tag="VWZ30_001389"
                     /pseudo
     CDS             8465..8688
                     /gene="tnpA"
                     /locus_tag="VWZ30_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            8792..8992
                     /gene="tnpA"
                     /locus_tag="VWZ30_001390"
                     /pseudo
     CDS             8792..8992
                     /gene="tnpA"
                     /locus_tag="VWZ30_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            9596..10216
                     /locus_tag="VWZ30_001391"
     CDS             9596..10216
                     /locus_tag="VWZ30_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335112.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIATLIFF"
     gene            10302..10745
                     /gene="tsaE"
                     /locus_tag="VWZ30_001392"
     CDS             10302..10745
                     /gene="tsaE"
                     /locus_tag="VWZ30_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU9335113.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDTYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            10735..11253
                     /locus_tag="VWZ30_001393"
     CDS             10735..11253
                     /locus_tag="VWZ30_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9335114.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            11261..12277
                     /locus_tag="VWZ30_001394"
     CDS             11261..12277
                     /locus_tag="VWZ30_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU9335115.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGVELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRVHMLEMQNILRRSLGQEEVTQLETNVVLFEDLFGRAPVG
                     DEDN"
     gene            12361..13617
                     /locus_tag="VWZ30_001395"
     CDS             12361..13617
                     /locus_tag="VWZ30_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU9335116.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGASSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGVSLTGVAGPDSLEGHPVGTVFIGLAQEQETEVINVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            13672..14838
                     /gene="recA"
                     /locus_tag="VWZ30_001396"
     CDS             13672..14838
                     /gene="recA"
                     /locus_tag="VWZ30_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU9335117.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEASEQDIENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
BASE COUNT         4488 a         2818 c         3484 g         4109 t
ORIGIN      
        1 tgaaaatgat tttcagtcaa tagttttttc agttttttgt accatagcgt gattcgaatg
       61 tgatgccaag ttttctatgg gaaaaaaatt accaaaaacc tactgaattc ccttgaaaaa
      121 tctagctaaa tagggtataa tatgggtaat catttgtcgt aggttttgtc tgaaatattg
      181 tccagacaag gctcacagca gttaaatctt ctgaaaaagt cagatttagc tgctcttttt
      241 gtgctttttt tcaagatttt gagcatttgt aacagaggct taaagattct gaaaattcgt
      301 caagaggaca cggtgataag gggtttacaa ccatatggcg attagaaaag cctgattgac
      361 aaggcttgga acttatttac aaaggagaat catcttggca ggacatgacg ttcaatacgg
      421 gaaacatcgt acccgtcgta gtttttcaag aatcaaagaa gttcttgact taccaaattt
      481 gattgaaatt caaactgact cattcaaagc tttcctagac cacgctctta aggaagtgtt
      541 tgaagatgta ttgccaattt caaacttcac agacacaatg gagttggaat ttgttggata
      601 tgaaatcaag gaaccaaaat acacgctaga agaagctcgt atccacgatg ctagctactc
      661 agcaccaatt tttgtaacct tccgcttgat caataaagaa acaggcgaaa tcaagaccca
      721 agaagttttc tttggtgatt tcccaatcat gacagaaatg ggtactttca tcatcaatgg
      781 tggtgaacgt attatcgttt ctcagttggt ccgctcacca ggtgtttact ttaacgacaa
      841 agtagacaaa aatggtaagg tgggctatgg ttcaactgtt atccctaacc gtggagcttg
      901 gttggaactt gaaagcgact caaaagatat cacctacact cgtatcgacc gtactcgtaa
      961 gattccattt acaaccttgg ttcgtgctct tggtttctca ggtgatgatg aaatctttga
     1021 tatctttggt gacagcgaat tggttcgcaa cactgttgaa aaagatatcc acaagaatcc
     1081 aatggactct cgtacagacg aagccttgaa agaaatttac gaacgccttc gtccaggtga
     1141 gcctaagaca gctgaaagct cacgtagctt gcttgtggct cgcttctttg acccacgtcg
     1201 ctatgacttg gcagcagttg gtcgttacaa aatcaataaa aaactcaatg ttaaaacacg
     1261 tttgctcaac caaaccattg cagagccatt ggtagaccct gaaactggag aaatcttggt
     1321 agaagctggt acgattatga ctcgtagcgt gattgaaagt attgaaagcc atttggatgg
     1381 cgacttgaac aagattgtct acatcccaaa cgatgcagcc gttgtgactg agcctgttgt
     1441 tcttcaaaaa ttcaaggttg ttgctccaac tgatccagat cgcgtcgtaa cgatcattgg
     1501 taatgctaac ccagatgaca aggttcgtac ggtgactcct gcagatatcc ttgctgagat
     1561 gagctacttc ctcaacttgg ctgaaggact tggccgtgta gatgatatcg accaccttgg
     1621 aaatcgtcgt atccgtgcgg ttggtgaatt gcttgccaac caagtacgtt tgggactttc
     1681 tcgtatggaa cgtaatgtcc gtgaacgtat gtctgttcag gacaatgaag tcttgacacc
     1741 acaacaaatt atcaatatcc gtcctgtaac agctgcagtt aaagaattct ttggttcatc
     1801 acagttgtca cagttcatgg accaacacaa cccgctttct gagttgtctc acaaacgccg
     1861 tttgtcagcc ttaggacctg gtggtttgac tcgtgaccgt gccggatatg aagtacgtga
     1921 cgtgcactac actcactatg gtcgtatgtg tccaatcgag acacctgaag gacctaacat
     1981 cggtttgatc aataacttgt catcttacgg acacttgaac aaatatggtt ttgttcaaac
     2041 accataccgt aaggttgacc gtgaaacagg tgttgtcacg aacgaaattg tttggttgac
     2101 agctgatgaa gaagatgaat atactgtagc tcaggctaac tctcgtctga atgaagatgg
     2161 aacctttgct gagaagattg tcatgggacg tcaccaaggg gtcaaccaag agtatccagc
     2221 taatattgtt gactacatgg acgtttcacc aaaacaggta gttgccgttg cgacagcatg
     2281 tattcctttc ttggaaaacg atgactccaa ccgtgccctc atgggagcca atatgcaacg
     2341 tcaggctgtg ccattgatta atcctcaggc accttacgtt ggtactggta tggaatacca
     2401 agcagcccac gattctggtg cggctgtgat tgctcagtat gatggtaaag ttacttacgc
     2461 agatgctgac aaggtagaag ttcgtcgtga agatggttca ttggatgttt accacatcca
     2521 aaaattccgt cgttcaaact caggtactgc ttacaaccaa cgcactctcg taaaagttgg
     2581 tgatgtcgtt gaaaaaggcg atttcatcgc tgacggacct tctatggaaa atggagaaat
     2641 ggcgcttgga caaaacccaa tcgttgccta catgacttgg gaaggttaca acttcgagga
     2701 tgccgttgtc atgagcgaac gcttggtgaa ggacgatatc tacacatctg ttcaccttga
     2761 agaatacgaa tcagaaacgc gcgatacaaa gcttgggcct gaagaaatca ctcgcgaaat
     2821 tccaaacgtt ggtgaagatg ccctcaaaga ccttgacgaa atggggatta tccgtattgg
     2881 tgctgaggtt aaagaaggtg atattcttgt aggtaaagta acacctaagg gtgagaaaga
     2941 cctttcagct gaagaacgtc tcttgcacgc tatctttgga gacaagtctc gtgaagtgcg
     3001 tgatacttct cttcgtgtac cacacggtgc cgatggtgtc gttcgtgatg ttaagatctt
     3061 tacacgtgta aatggagatg agttgcaatc aggtgttaac atgttggttc gtgtttacat
     3121 cgctcaaaaa cgtaagatta aggtcggaga taaaatggcc ggacgtcacg gaaacaaagg
     3181 ggttgtctct cgtatcgttc ctgtagaaga catgccttac cttccagacg gaactccagt
     3241 cgacatcatg ttgaatccac ttggggtgcc atcacgtatg aatatcggtc aggttatgga
     3301 gcttcacctt ggtatggcag ctcgtactct tggtattcac atcgcgacac cagtctttga
     3361 tggagcaagt tctgaagatc tttggtcaac tgttaaagaa gcaggtatgg atagcgatgc
     3421 caagacaatc ctttacgatg gacgtacagg tgaaccattt gataaccgtg tttctgttgg
     3481 agtcatgtac atgatcaaac ttcaccacat ggttgacgat aaattgcacg cacgttcagt
     3541 cggaccttac tcaactgtta cccaacaacc actcggaggt aaagctcagt ttggtggaca
     3601 acgtttcggt gagatggagg tttgggctct tgaagcctac ggtgcgtcaa atgtccttca
     3661 agaaatcttg acttacaagt ctgacgatat caacggacgt ttgaaagcct atgaagctat
     3721 tacaaaaggc aaaccaattc caaaaccagg tgttccagaa tccttccgag ttcttgtcaa
     3781 agaattgcaa tctcttggtc ttgacatgcg tgtcctagac gaagatgacc aagaagtgga
     3841 acttcgcgac ttggatgaag gaatggacga agatgtcatc cacgtagatg accttgaaaa
     3901 agcccgtgaa aaagcagccc aagaggctaa agcagccttt gaagctgaag acgctgagaa
     3961 agcaacaaaa gcggaagcaa cagaagaagc tgctgaacaa gaataagcag ttcacttacg
     4021 aatagaaagg gaagaaatag tggttgatgt aaatcgtttt aaaagtatgc aaatcaccct
     4081 agcttctcca agtaaagtcc gttcatggtc ttatggagaa gtcaaaaaac ctgaaacaat
     4141 caattatcgt accttgaaac cagaacgtga aggactcttt gatgaagtga tctttggtcc
     4201 tacaaaagac tgggaatgtg cttgtggtaa gtacaaacgc attcgttaca gaggaattgt
     4261 ttgtgaccgc tgtggggttg aagtaacgcg tacgaaagtt cgtcgtgagc gtatgggaca
     4321 tatcgaattg aaagctcctg tatctcacat ctggtacttc aaggggattc caagccgtat
     4381 gggcttgacc cttgatatga gccctcgtgc cctcgaggaa gttatctact ttgcggctta
     4441 tgtggtgatt gatcctaagg atacaccact tgagcacaag tctatcatga cagagcgcga
     4501 ataccgagag cgcttgcgcg aatatggtta tggttcattt gttgccaaga tgggtgccga
     4561 agccatccaa gaccttttga agcaagtaga tcttgaaaaa gaaattgctg aactcaaaga
     4621 agaattgaaa acagctaccg gacaaaaacg tgttaaagcc atccgtcgtt tggatgtttt
     4681 ggatgccttt tacaagtctg gaaacaaacc tgaatggatg atccttaaca tccttccggt
     4741 tatcccacca gatcttcgtc caatgttgca gttggatggt ggccgttttg cctcatctga
     4801 cttgaatgac ctttaccgcc gtgttatcaa ccgtaacaac cgtttggctc gtttgcttga
     4861 gttaaatgca ccaggtatca tcgttcaaaa tgagaagcgt atgcttcaag aagcggttga
     4921 cgctttgatt gacaatggtc gccgtggtcg tccaatcaca gggccaggta gccgtccatt
     4981 gaaatcattg agccacatgc ttaaaggtaa acaaggacgc ttccgtcaaa acttgctcgg
     5041 taaacgtgtt gacttctcag gacgttccgt tatcgccgtt ggtccaactc ttaagatgta
     5101 ccaatgtggt gtgccgcgtg aaatggcgat tgaactcttt aaaccatttg tcatgcgtga
     5161 aatcgttgcc cgtgatatcg tgcaaaacgt caaagcagct aaacgcttgg tggaacgcgg
     5221 agatgagcgt atctgggata tccttgaaga agtgattaaa gaacacccag tgcttttgaa
     5281 ccgcgcaccg acccttcacc gtttgggtat ccaagccttc gagccagtct tgattgatgg
     5341 taaggctctt cgcttgcacc cacttgtctg tgaagcctac aatgccgact ttgacgggga
     5401 ccaaatggcc atccacgtac cactttcaga agaagcacaa gcagaagctc gtatcctcat
     5461 gctagctgct gagcacatct tgaacccgaa agatgggaaa ccggtagtta ctccatctca
     5521 ggacatggtt ttgggtaact actacttgac catggaagaa gctggtcggg aaggcgaagg
     5581 aatggtcttc aaagatcgtg acgaagcggt tatggcttac cgcaatggtt atgttcacct
     5641 ccactcacgt gttggtatcg caacagacag cctcaacaag ccttggacag aagagcaaag
     5701 acataaggtc ttgcttacaa cagttggtaa agttctcttc aacgatatca tgccagaggg
     5761 gctaccatac ttgcaagaac caaacaatgg caacttgaca gaaggtgttc cagctaaata
     5821 cttcttgcca cttggtggag atatcaagga agctatcagc aatcttgaac tcaaccctcc
     5881 attcaagaag aaaaaccttg gaaatatcat cgctgaaatc ttcaaacgtt tccgtacgac
     5941 agaaacttct gccctacttg accgcatgaa gaacctcggt taccagcact caactcttgc
     6001 aggattgaca gtgggtattg ccgatatccc agtcgttgat gacaaggctg aaatcattga
     6061 agaatcacac aaacgtgtcg aacaaatcac caaacaattc cgtcgtggta tgatcacaga
     6121 cgacgagcgt tacaatgctg ttacagctga atggcgtgct gcccgtgaaa aacttgagaa
     6181 acgcttgatt gctgaccaag atcctaagaa cccaatcgtt atgatgatgg actctggagc
     6241 ccgtggtaac atctcaaact tctcacagct tgccggtatg cgtggtctga tggctgctcc
     6301 gaatggacgt atcatggaat tgccaatcct ttcaaacttc cgcgaaggtt tgtcggtatt
     6361 ggaaatgttc ttctcaactc acggtgctcg taaaggtatg accgatacgg cccttaagac
     6421 agccgactca ggttacttga ctcgtcgttt ggtcgacgtt gcccaagacg ttatcatccg
     6481 tgaggacgac tgtggaactg accgtggtct cttgatccgt tctatcgcag aaggaaaaga
     6541 gatgatcgag tctctcgaag agcgtctcaa cggtcgttac actaagaaaa ctgttaaaca
     6601 tccagaaact ggtgcagtga ttattggtcc aaatgaattg attacagaag acaaggcgcg
     6661 tgaaattgtc aatgctggtg tggaagaagt gactatccgt tctgtattta catgtaacac
     6721 tcgtcacggt gtctgccgtc actgttacgg tatcaacttg gcgactggtg atgcggttga
     6781 agttggtgaa gcagttggta caatcgctgc ccaatctatc ggggaacctg gtacacagct
     6841 tacaatgcgt accttccaca caggtggggt tgcctcaaat accgatatca ctcagggtct
     6901 tcctcgtgtc caagaaatct ttgaagcccg caatcctaaa ggggaagcgg ttatcacaga
     6961 ggttaaagga caagttactg ctatcgaaga agatgcatca actcgtacca agaaagtctt
     7021 tgttaagggt gaaactggcg aaggtgaata tgtcgttcca tttacagctc gtatgcgtgt
     7081 cgaagttggg ggccaagtag cgcgtggtgc tgctctgaca gaaggttcta tccaaccaaa
     7141 acgtctcctt gcagttcgtg atgtcttgtc agttgaaacg taccttctcg gtgaagtaca
     7201 aaaagtttac cgtagccaag gggtagaaat cggtgacaaa cacatcgagg taatggttcg
     7261 tcaaatgatc cgtaaagtcc gtgtcatgga tccaggtgat acagatcttc tcatgggtac
     7321 cctcatggat atcaatgact ttacagatgc caacaaagat gtccttatcg caggtggagt
     7381 tccagcgaca ggtcgcccag tccttatggg aattaccaaa gcctcacttg aaacaaacag
     7441 tttcttgtca gcggcttcct tccaggaaac aactcgtgtc cttactgacg cagctatccg
     7501 tggtaagaaa gaccatctcc ttggacttaa agaaaatgtt atcatcggta agatcatccc
     7561 agctggtact ggtatggccc gttaccgtaa ccttgaacca catgctgtca acgaagaaga
     7621 ataccttaat cctccagtag aggaagaagg aaatgaagaa acaacagaag tagttgtgga
     7681 tactgccgtt gaaactgtgg aagaaacagt agaataagag aaccctcggg ttctctcatt
     7741 ctctcttctg aaaactttct ccatttttcc acgaaatgtg gtaaaataaa agaaagaagc
     7801 tgacaaagga gacgagtatg gaacaaacat tctttatcat caaaccagat ggtgtaaaaa
     7861 gaggactagt gggtgaagtg ttaaagcgca tcgaacaacg tggatttaca atcgaaaaat
     7921 tggagtttcg ttcacaggtt tcagaagagt tgattgacca gcactatcag gacttggttg
     7981 gtcagagttt ttacccaccg attcgtgaat tcatgacttc aggtccagtt cttgtgggtg
     8041 tcatttctgg taccaaagta atcgaaattt ggcggaccat gatgggtgca actcgtccag
     8101 aagaagcttt accaggcact attcgaggtg attttgcaaa agctgctgaa gaaaatgagg
     8161 ttatccaaaa tgttgtacat ggttcagatt cagaagagtc agctaagcga gaaattgctc
     8221 tttggtttta agagtggatt ggctcaatca attggttaaa agctcatttg aatagaaagt
     8281 atagtcaatt agtttaagac atgacgcatg atatcaaact ttttaggttt tgatatggtg
     8341 cgttttttga ttaagtgact attagttcgt ctcgctccgt taggtgcgag acaaaacaac
     8401 cacccgctat gcgggtgcgc gtcgaaggtt ataccaaaaa aactccaaac gcgatacaat
     8461 aaaggtgttc aagccaatta taaagcgaaa ggagaaaaat atggcacaaa aagcacatag
     8521 tttatcatac acaaagtgga tgtgttctat cacattgtgt tcacccctag gtatagacga
     8581 acagtcattt ataatcaata tcgaagtagt ttgggagaaa tatttcatcg cttgtgtagt
     8641 tataaaggtg ttgaaattat cgagggtcac ttaatgcctg aacatgtata gtgaattgaa
     8701 ataagatgtg aacatcctct atcaggaaag tcaaattaat ttatagaaat attttagcag
     8761 tcaagatgta ctgttatagc ttcaatacac tatacatatg ttagtcagta ttccgccgag
     8821 gctaagtgtt tcaagtttta tggggtgttt aaaaggcaaa agtgcactca tgatgtttga
     8881 caaacatgcc aatctcaagt acaagtttgg gaatcggcat ttctgggcgg aaggttatta
     8941 tgtgagcaca gtagggctta acaaagccac aattaagaag tatagtcaag attaaagaaa
     9001 ttatcgagtg gatgatttct tcacgagtat gaaaatttga gaacgagtaa agcatgatat
     9061 atagtaaaat gaaataagaa caggacaaaa ttgatcagga cagtcaaatc gatttttaac
     9121 aatgttttag aagtagaggt gtactattcc agtttgaatc tactatagca ctagataaat
     9181 tgagtgtaaa agaatatgag gctcccttta gggatagtgg taagtaatac taaagcctct
     9241 taaagaggca agtgacgagt caagagcaat aaggcttgaa caacgtgaaa gccagcgcct
     9301 aaagacgctg gctgatgatt tgggcttata gctctaggat aaaccacccg tttgacggat
     9361 tttcatgatt tcaaatttat attcttcaaa aatctcttca aaccacgtca gctctatctg
     9421 caatctcaaa acagtgtttt gagcaacctg tggctagcta cctagtttgc tctttgattt
     9481 ttattgagta ttagtctcta gtgtttgttt agcgtcttaa agagacattt gagaagatta
     9541 gaaacagttc tctcttttct cctagtataa tgattggtag gaagggagag agaagatgaa
     9601 atcaatgaga atcttatttt tgttagcttt aattcaaatc agtttgagta gctgtttcct
     9661 atggaaggaa tgcatcttgt cctttaaaca aagtacagct tttttcatcg gaagcatggt
     9721 tttcgtttca ggaatctgtg ctggagtaaa ttatctttat acccgtaagc aagaagtcca
     9781 tagtgtccta gccagtaaga agtcggtgaa gcttttttat agtatgttac tcttaattaa
     9841 tttgttagga gctgttcttg ttttgtcaga taacttgttc atcaaaaata cgctgcagca
     9901 agaattagtt gactttttat tgccatcctt ctttttccta tttggactag atttgctgat
     9961 ttttttaccc ttgaaaaaat acgtgcgcga ttttcttgct atgctagaca gaaaaaagac
    10021 agtgttggtg actattttag caacacttct tttcttaaga aatccaatga ccattgtctc
    10081 acttctgatt tatattggac tgggcttgtt ttttgcagcc tatcttgtcc caaattcggt
    10141 taagaaggaa gtttcctttt atggtcatat tttccgagat cttgtattgg tcattgctac
    10201 gctcattttc ttttagagaa agcctgtttt ttatggatgt tatgatgata agtttagtag
    10261 gaaaatagac acaaaagaaa agtttttggt ataataagag tatgtacaca aaaaatgaag
    10321 aagagttgca agccttaggg gagcgtttgg gccatctatt agcaaagaat gatgttttaa
    10381 tcttaactgg agaactgggt gcaggtaaaa cgacctttac taaaggactt gcaaaaggat
    10441 tacagatttc tcaaatgatt aaaagtccca cctatactat cgtgagagag tatgaaggtc
    10501 gacttccact ttatcaccta gatgtttatc gtattgaagg agatgctgat tctatcgact
    10561 tggatgagtt tatctttggt ggtggcgtga ctgttattga gtggggaaat ctcttaggag
    10621 atgccttgcc agatacttat ttagaattgg aaattctaaa agaagcagat ggacgccgtt
    10681 taaattttca ggcaaagggt ttgcgtgctg agaaattgtt agaggagctt caatatggag
    10741 tatgaattgc tcattaggga agcagagccc aaagatgcag ctgaattagt ggccttttta
    10801 aatcgtgtga gtttggagac agactttacc agcctagacg gagatggtat tctcttgact
    10861 agtgaggaga tggaaatatt cctcaacaag caagctagtt cggacaatca gataacctta
    10921 cttgcattct taaatggcaa aattgctggt attgtaaata ttacagctga ccagcgtaag
    10981 agagtccgtc atattggaga tctcttcatt gtgattggaa aaagatattg gaataatggc
    11041 ttgggaagtt tgttgctaga agaagcgata gagtgggcac aagcaagtgg cattctgcgt
    11101 cgtctccaac tgactgtcca aactcgtaat caagcagcag tccatcttta tcaaaagcat
    11161 ggctttgtca ttgaaggtag ccaagagcgt ggggcatata tagaagaagg gaaatttatc
    11221 gatgtttact tgatgggtaa actgataggt tagtagaaat atggttaaaa aaattattgg
    11281 aatggtgcta gctttacttt ctgtaactgt agtaggagta ggtgtttttg cttatactat
    11341 ttatcaacaa gggacagaaa ccttagctaa aacctataaa aaaatcggcg aagaaaccaa
    11401 ggttattgaa gcgactgaac ctctaaccat tctgttaatg ggagtggaca ccggaaatgc
    11461 tgaacgaact gaaacttggg tcggtagaag tgatagcatg atcttgatga cagtgaatcc
    11521 taaaacgaaa aaaacaacaa tgatgagttt agagcgggac attctgacgc gcattgaatc
    11581 agggaatggt caggctcatg aagcgaaact gaactcagca tatgcagatg gtggagtaga
    11641 gcttgctata gaaaccattc aaaaaatgat gaatatccat attgatcgct atgtaatggt
    11701 caatatgaga gggttgcaaa aactagtgga tgcagtagga ggtattacag tcaataatat
    11761 cctaggtttc ccaatttcta tcagtgacca agaagaattt aatactattt ctatcggtgt
    11821 tggggagcaa catattgggg gagaagaagc cctagtctat gcacgaatgc gttaccaaga
    11881 tcctgagggg gattatggtc gtcaaaaacg tcaacgtgaa gttattcaaa aagtcatgga
    11941 aaaagctctc agtttaaata gcgttggtca ttatcaagag attctaaaag ctttgagtga
    12001 caatatgcag accaatattg atttgtctgc aaaaagtatc cctaacttgc taggctataa
    12061 agattcattt aaaaccattg aaactcagca gttgcagggt gaaggagaga tacttcaagg
    12121 tgtttcttac cagattgttt caagagtaca tatgttggaa atgcaaaata tactccgacg
    12181 ttctttggga caagaagaag ttactcagct tgaaaccaat gtggttttat ttgaagattt
    12241 atttggcaga gcacctgttg gtgatgaaga taattaactt ttgatacaaa taaaaaatca
    12301 atcgtaggaa aattcctgcg attttttcaa aaaaatacga atagataggt aggaggaaat
    12361 atgaaagcag aaatcattgc tgttggaaca gagattttga caggacagat tgtcaacacc
    12421 aatgcccagt ttttgtcgga aaaactagct gagattgggg tagatgtata ttttcagaca
    12481 gctgtaggag acaatgaagt tcgtctcttg tctttgcttg agattgccag tcaacgtagc
    12541 agtctggtga ttttgacagg cggtttgggg ccaactgagg acgacctaac caaacaaacc
    12601 ctagctaaat ttttagggaa agcattagtc tttgatcctc aggctcagga gaagttggat
    12661 atcttttttg ccctgcgacc agactatgcc cgaacaccga ataacgaaag acaagctcaa
    12721 attgtagaag gagcgattcc actgccaaac gaaacaggac tggctgttgg aggaaaatta
    12781 gaagtagacg gagtgaccta tgtcgtcctt ccaggtccgc caagtgaatt gaaacccatg
    12841 gtcttaaacc aacttctacc caagttgatg acagggagca agctgtattc ccgagttctt
    12901 cgtttctttg ggattggcga gagccagctg gttacgattt tggctgattt aattgataat
    12961 cagatcgatc ctaccttggc cccttatgcc aagacaggag aagtcactct acgtctgtca
    13021 acaaaggcta gcagtcaaga agaggcgaat caagcgctgg atatcttgga aaatcaaatc
    13081 ttggactgcc agactttcga aggaatttct ttacgagact tttgctatgg ttatggggaa
    13141 gaaactagtt tagccagcat tgtggtagaa gaactgaaaa ggcaagggaa aaccatcgcg
    13201 gctgcagaga gtttgacggc aggtcttttc caagctaccg tggcgaattt ttctggagct
    13261 tcaagtatat ttaagggtgg ttttgtgacc tatagcttgg aggaaaaatc aaggatgttg
    13321 gatattcctg ccaagaattt ggaagaacat ggtgtggtgt ctgaatttac agctcagaag
    13381 atggctgagc aggcacgaag caagacccag tctgattttg gagttagttt gactggagtg
    13441 gcaggaccag atagcctaga aggacaccca gttgggacag tcttcatagg cttggcgcaa
    13501 gagcaagaaa ctgaggttat caatgttaat attggaggca gaagccgagc agatgtacgt
    13561 catattgcgg ttatgcatgc ctttaaccta gttcgcaagg ctttattaag tgactaactt
    13621 ttgatataat agtagatagg tctgaggaac attagaatgt aggagaatag aatggcgaaa
    13681 aaaccaaaaa aattagaaga aatttcaaaa aaatttgggg cagaacgtga aaaggccttg
    13741 aatgacgctc ttaaattgat tgagaaagac tttggtaaag gatcaatcat gcgtttgggt
    13801 gaacgtgcgg agcaaaaggt gcaagtgatg agctcaggtt ctttagctct tgacattgcc
    13861 cttggctcag gtggttatcc taagggacgt atcatcgaaa tctatggccc agagtcatct
    13921 ggtaagacaa cggttgccct tcatgcagtt gcacaagcgc aaaaagaagg tgggattgct
    13981 gcctttatcg atgcggaaca tgcccttgat ccagcttatg ctgcggccct tggtgtcaat
    14041 attgacgaat tgctcttgtc tcaaccagac tcaggagagc aaggtcttga aattgcggga
    14101 aaattgattg actcaggtgc agttgatctt gtcgtagtcg actcagttgc tgcccttgtt
    14161 cctcgtgcgg aaattgatgg agatatcgga gatagccatg ttggtttgca ggctcgtatg
    14221 atgagccagg ccatgcgtaa acttggcgcc tctatcaata aaaccaaaac aattgccatt
    14281 tttatcaacc aattgcgtga aaaagttgga gtgatgtttg gaaatccaga aacaacaccg
    14341 ggcggacgtg ctttgaaatt ctatgcttca gtccgcttgg atgttcgtgg taatacacaa
    14401 attaagggaa ctggtgacca aaaagaaacc aatgtcggta aagaaactaa gattaaggtt
    14461 gtaaaaaata aggtagctcc accgtttaag gaagccgtag ttgaaattat gtacggagaa
    14521 ggaatttcta agactggtga gcttttgaag attgcaagcg atttggatat tatcaaaaaa
    14581 gcaggggctt ggtattctta caaagatgaa aaaattgggc aaggttctga gaatgctaag
    14641 aaatacttgg cggagcaccc agaaatcttt gatgaaattg ataagcaagt ccgttctaaa
    14701 tttggcttga ttgatggaga agaagcttca gaacaagata ttgaaaacaa aaaagatgag
    14761 ccaaagaaag aagaagcagt gaatgaagaa gttacgcttg acctaggcga tgaacttgaa
    14821 attgaaattg aagaataagc tgttaaagta gtggagaaat ccgctacttt ttcgattttt
    14881 gattcaagtt tttagatta
//
LOCUS       DAVKDZ010000045        14867 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000045 DAVKDZ010000000
VERSION     DAVKDZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14867
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.046"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            1..1069
                     /locus_tag="VWZ30_001397"
                     /pseudo
     CDS             1..1069
                     /locus_tag="VWZ30_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(1135..3015)
                     /gene="cbpE"
                     /locus_tag="VWZ30_001398"
                     /gene_synonym="pce"
     CDS             complement(1135..3015)
                     /gene="cbpE"
                     /locus_tag="VWZ30_001398"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEU9335118.1"
                     /translation="MKKKLTSLVLAGAFLSLSWYGNVQAQESSGNKIHFINVHEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWRQGINTTYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYKNETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGSLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSAYGNWWYQAPDSTGEYVVGWNKIEGEWYYFNQTGILLQNQWKKWNNHWFY
                     LTDSGASAKNWKKIDGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSNSEIKESNQVVKKDLENKETNQHESVASSSSSQDLTSSTSQSSEINANKSE
                     SE"
     gene            complement(3018..3905)
                     /locus_tag="VWZ30_001399"
     CDS             complement(3018..3905)
                     /locus_tag="VWZ30_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9335119.1"
                     /translation="MEIKIETGGLRLDKALSDLTELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDIPLEIVYQDEDVVVVNKPQGIVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYNLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGSRKTLKGHGQFLHAKTLGFTHPRTGKTM
                     EFTADIPEIFKETLERLRK"
     gene            complement(3895..4356)
                     /locus_tag="VWZ30_001400"
     CDS             complement(3895..4356)
                     /locus_tag="VWZ30_001400"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU9335120.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILSIAMLKEEINGN"
     gene            complement(4353..5261)
                     /locus_tag="VWZ30_001401"
     CDS             complement(4353..5261)
                     /locus_tag="VWZ30_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9335121.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLREGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            5254..5451
                     /locus_tag="VWZ30_001402"
                     /pseudo
     CDS             5254..5451
                     /locus_tag="VWZ30_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(5503..6174)
                     /locus_tag="VWZ30_001403"
     CDS             complement(5503..6174)
                     /locus_tag="VWZ30_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9335122.1"
                     /translation="MKKRAIQILLVLSLIFYKSTWFWRLFNYLAKPYLPASREFFQIL
                     LLMESGVLLLAVIYLLVFAGKKTFHFKWQLRYFIYLLLGYITSYMSDFLFSYFISLSS
                     NQISLNETIEMIGRQELPYFLLIVCFIGPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(6228..6449)
                     /locus_tag="VWZ30_001404"
     CDS             complement(6228..6449)
                     /locus_tag="VWZ30_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEU9335123.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            6721..7530
                     /locus_tag="VWZ30_001405"
     CDS             6721..7530
                     /locus_tag="VWZ30_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9335124.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(7711..8586)
                     /gene="aguB"
                     /locus_tag="VWZ30_001406"
     CDS             complement(7711..8586)
                     /gene="aguB"
                     /locus_tag="VWZ30_001406"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU9335125.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(8596..9681)
                     /gene="aguA"
                     /locus_tag="VWZ30_001407"
     CDS             complement(8596..9681)
                     /gene="aguA"
                     /locus_tag="VWZ30_001407"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEU9335126.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVTDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(9678..10805)
                     /gene="nspC"
                     /locus_tag="VWZ30_001408"
     CDS             complement(9678..10805)
                     /gene="nspC"
                     /locus_tag="VWZ30_001408"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEU9335127.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLEITDHIVF
                     NSERQLRKHGPRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(10805..12064)
                     /locus_tag="VWZ30_001409"
     CDS             complement(10805..12064)
                     /locus_tag="VWZ30_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEU9335128.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGQWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQDIVPIQFLKALLPAPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(12061..12921)
                     /gene="speE"
                     /locus_tag="VWZ30_001410"
     CDS             complement(12061..12921)
                     /gene="speE"
                     /locus_tag="VWZ30_001410"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU9335129.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMTVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GYTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQTFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTKYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            complement(12922..14382)
                     /locus_tag="VWZ30_001411"
     CDS             complement(12922..14382)
                     /locus_tag="VWZ30_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU9335130.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILS
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENVRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTDKLPISAMDAG
                     ADIAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDFLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKEN"
BASE COUNT         4420 a         3332 c         2661 g         4454 t
ORIGIN      
        1 tcaagtgcta tcactgttct aaaatgatgg tcgctgaaac ttctatcgtc aagaagaatc
       61 accaaatccc tcgtatcatc aaccaaaaga ttgctcaaaa gttaattgaa aagatttcta
      121 tgactgatat tgcccatcag ctttccatct caacttcaac tgttattcgt aagctcaatg
      181 attttcactt taaacatgat ttttcttgtc ttcctgagac tatggcttgg gatgagtatg
      241 cttttacaaa gggaaagatg agcttcattg cgcaagattt taacaatctc aatatcatca
      301 ctgttcttga gggtagaaca caagctatca ttcgaaatca ctttcttcgc tacgatagag
      361 ctgtccgatg tcgcgtcaaa attattacta tggatatgtt tagtccttac tatgacttgg
      421 ctaaacagct tcgctttcaa atttctaggc tcaggctgaa acagtctccc aaactgtttc
      481 actcccgaat gctaaaatcg ttcttgatcg ctttcacatt gtacaacatc ttagccgtgc
      541 tatgagtcgt gtgcgtgttc aaatcatgaa tcaattggat cgaaagtctc atgaatacaa
      601 ggctatcaag cgctactgga agctcataca acaggatagt cgtaaactca gcgataaaca
      661 tttttatcgc cctacttttc gtatgcattt aaccaataaa gagattttag acaagctttt
      721 gagctattca caagacttga aacatcacta tcagctctat caactcttgc tgtttcactt
      781 tcagaataag gaaccggaga aatttttcgg actcattgag gacaatctaa agcaggttca
      841 tcctcttttt cagactgtct ttaaaacctt tctaaaggac aaagagaaaa tcgtcaacgc
      901 ccttcaacta ccctattcaa acgccaaatt ggaagcgacc aataatctca tcaaacttat
      961 caagcgcaat gcctttggtt ttcggaactt tgaaaacttc aaaaaacgga tttttatcgc
     1021 ccttaatatc aaaaaagaaa ggacaaaatt tgtcctttct cgagcttagc tgacttcaac
     1081 ccactacagt tgacaaagag ccgaaaaaga aggccctaaa accttctttt actactattc
     1141 tgattccgat ttgtttgcat ttatttcaga gctttgtgaa gttgatgaag ttaaatcttg
     1201 actagatgaa gaacttgcaa cactttcatg ttgattcgtt tctttatttt caagatcttt
     1261 tttcactact tgattggatt cttttatttc tgaattactt gtagttttaa ttgtagcaga
     1321 tttctcgatc ggaacatcca ctaaccaggc accacttgaa tccacagtat agccttctgg
     1381 tgtcttacca ttcacaagta attgaccatt ttctttcaag aagtaccatt tatccttgtc
     1441 tttaatccaa cctatagctc gtgaaccatc tgtcttgtag aaataccaat catttgcctt
     1501 tttaagccag ccttgcttca tagctcctga ttcttctaga tagtaatgat gtccagcaac
     1561 ttctatctca cctgtcttca tgataccagt agaatccata tagtaccagg cttctttatc
     1621 ttttacccag cccgttttca tttcccctga tggagcaaag taataccatt gccccttata
     1681 ttgaagccaa cctgtcttca tagagccatc aacatccaaa taataccact gctctttatc
     1741 ttgaatccaa ccaatttcca tctggttttc tttgttaaaa taataccaga ttccatcaat
     1801 tttcttccaa tttttagcag aagcaccaga gtctgtcaaa tagaaccaat gattgttcca
     1861 ttttttccat tgattctgta acaagatacc cgtttggtta aagtaatacc attcaccttc
     1921 gattttattc caaccgacaa catactctcc tgtagaatca ggcgcttgat accaccagtt
     1981 cccatatgca ctcttatgcc aaccagcttg aaaacttgga atcggcttgt aggatgttga
     2041 aatattgaca aaaccgtctt ttcgaatatc aaaaactgtc gcatcatagt ctttgctggc
     2101 tgcgttaatt ctctcaattc ctcgttcttt gagccaatta acatactcac tatcaacacc
     2161 atttttccaa ggtagactat ccgaagtttg aacaatcaaa ctcggactca aatttttaat
     2221 aaaatccttg gtatttgatt tgttggtatc atggtgatga ttaaacttca tcaaatcaac
     2281 ttttccaatg agagaaccat acttgtcttc tgctccatga acattatcta agtcgccccc
     2341 aaggtaaatt ttcttgccat tgactttcac cacgctaatc aaggaattgg aattgtcatc
     2401 ccaaattttc tttaattcac ccgatgaatc agtttcattt ttataattat agagctgaat
     2461 atccatgtcc ccaaactgaa aatgagcatc cccttgtgtg atattttgaa taactgaaac
     2521 acctttttct gcagcagtct gtaaaacctt atcatagcca tacagattat cccatagacg
     2581 ttcagaatta gtaatacgac tatcactata tttcttaaga tagactcggt caactggata
     2641 ggtagacagt aattcatcaa catttccaat atgatcactg tgggtatggg tcaccaaaat
     2701 aaaatcaagt ttttggacac ccaattcctt caaacgacga aagacacggt ctgtcagaac
     2761 atgcttataa gtggtattga taccctgtct ccatggataa cgagaatcac ttccatctgg
     2821 gaaatcataa tcttctcctg tatccaccat ggcaaaatgt ccattgcttt caagaataat
     2881 cgcatcacta ccaccttcat gaacatttat aaagtggatt ttatttcctg aactttcttg
     2941 agcttgaaca ttcccatacc atgataaact taaaaaagcg cctgcaagta ctaaactagt
     3001 taatttcttt ttcattctta cttcctcaat ctctccaagg tttccttaaa aatctctggg
     3061 atatctgctg taaattccat ggtcttacct gttctcggat gagtaaaccc taaagtctta
     3121 gcatgaagaa attgtccatg tcctttcaga gtctttcgag aaccatagac ttcatcccca
     3181 gcgactggat gaccgatgta agccatgtgg acacgaatct gatgagtgcg ccctgtctcc
     3241 agttgcaatt ccactaagtt ataatcgcca aagcgttcca agacgtgaaa acgcgtcact
     3301 gcaggtttcc ctttagcagt tacagcctgt ttcttacggt ctttttcact ccggccaatc
     3361 ggcgcttcaa ttacaccacg atcattaggc agatttccat gaacaatcgc ccaatatttg
     3421 cggagagact ttttatcctt gagttcttgg gcaagtgcta gatgcgcatc atcgttttta
     3481 gcaatcatga gaagacctga cgtatcctta tcaatacggt gaacaattcc tggacgcaga
     3541 accccattga tacccgacaa gtccttaata tgatacatga gggcatttac tagggtccca
     3601 ctggtatgac cagcactcgg atgcacaact atcccctgag gtttgttaac gacaaccaca
     3661 tcctcatctt ggtagactat ttctagcgga atatcctcag ccacatactc taatacctct
     3721 ggttctggca catggtaagt gacgacatca ccctcttgga ctgtgtattt agctttcttg
     3781 acttgaccat tgaccaagac ctggcctgat ttaatttgtt cattcgcaag actgcgtgat
     3841 aattctgtca aatctgataa agccttatcc aaacgcagac caccagtttc aattttaatt
     3901 tccatttatt tcctctttta gcattgcaat cgataaaata atcactccaa ccgtcagata
     3961 gctatctgcc acattgaaaa ttgcaaagtt gataaagtca aggtggaaca tatccacaac
     4021 aaagccctga ctgaccctat caataaagtt tccaagacca cccgcgatta tcagagtcaa
     4081 acccaagacc atccagaatg agtcctccat gtgtttatgt aaataccaaa tggcacctat
     4141 cacgacaacc agagtaatga cagcgaataa caactgctga tcttgtaaga tagaaaaggc
     4201 tgcacctcga ttttgcaggt aggtcaagct aacgaaattg ggaatccagg agcgcacttc
     4261 acccagtgga atctgctgga cgatatagga tttgaccaac tgatccagcc caatcaaaag
     4321 cagtacaatg actgccacta ttgctctttt tttcatgatt tcctcttttg atcaaaatat
     4381 tcttgcatga cttctacgaa gagagtccca gcttgactaa gctccacttc ttcgcgttta
     4441 acatagacca tgcggttatc taggttatcc ttgagacgaa taactgtaat gccattaaca
     4501 ctgtcactat ctaaaaatcc agaacctgtc gcataggcgt ccgtccgttc caaaatacca
     4561 ttcaaagtgg cacggtctgt cacattaaac atctgtgagc ttgcactggt atcgacaaag
     4621 ttctctgaat aataaaggta ctcgtctttc tcttgagtga aacgaaccgt tggtaaatcc
     4681 gctaaatcct ccatgactaa ttcctctttc tgggctaaag gatgaccctc acggagataa
     4741 atatgggtat ggaaaggaat caattcgatg acctccagac ctaatttttc aacccgttgc
     4801 ataatcccct ttttattttg attgttgagg tagataatcc caatctcact atgcccttgc
     4861 gccacttcat ctaatatttg aacagtagtt gattcaaaaa tacggaagtt cttatagtca
     4921 ggatagcgct ctgaaaaggc cgtaatagtt ggtggcaaga agtcatagtg ctggctagca
     4981 acggaaaatt catctttttc ttcttcagga ttggcatact gattttgaaa aatatcaaat
     5041 cctttaacca attcttgcgc tttttcataa aattccatcc cacgacgggt caagaaagtc
     5101 cctgagctgg tccgacggaa aatcttaaaa cccaactctt tttccaaatc acgaacagaa
     5161 atagacagac tcggctgact aacatacatc ttttcagcag cttcacggaa agtaccacta
     5221 ttggcaatgg ccacaacata gcgtaattgt tgaatgttca tcttctaccc ctaacttctt
     5281 tatcttttca ttataccata ttttagaagt tttccaaaaa ggaaaaaaga acatcctatt
     5341 cttcttaact atcttcacta tctgcctttc tcaagccaac cttattttca aaatcaattc
     5401 aaaaaaataa ctggatacac accactacaa tatcgaaaag aaaaacgttg actatttgtg
     5461 aaaaaagaaa atgccggaaa gttccgacat tattttagta agctaacttc ctgaaaatag
     5521 gagcaaccac acatcccaga aggcagtgat attaatgaga atatgaacta gtattggata
     5581 gtagagattt ttagtcatgc gatacaatag agctaaaata agacctccac tagcataaat
     5641 gaaaaaagca agaggagtta aagcaaaatt gatatgaata taaccgaaaa taatagcaga
     5701 aagcaagaca tctccgtacc aaggcgagtt tttgaaaaag gttgtcataa gcacaccgcg
     5761 ataaatcaat tcctcagcaa tagggccgat gaagcaaacg atgagcaaaa aatagggtaa
     5821 ttcctgtctc cctatcattt ctatcgtttc attcaaagaa atttgatttg aagacagaga
     5881 tatgaaatac gaaaagagaa agtcagacat atacgaagtg atgtaaccca gtaaaaggta
     5941 gataaaatac ctcagctgcc acttgaaatg aaaagttttc tttcctgcaa aaactagtag
     6001 atagatgacc gctaataaaa gaactccact ctccatcaaa agcagaatct gaaaaaattc
     6061 acgacttgct ggtagatagg gctttgcgag ataattgaaa agcctccaaa accaagttga
     6121 tttgtaaaaa attaaggaca atactagtaa aatttgaata gctcgttttt tcatattaaa
     6181 tcctctgttt tctccccttg tttcttcata ttcctaataa attttactta aaatctcgca
     6241 gcacttcctt tgcaaagatg attgcctcct ccaatatatc ctaaatcata ggtgcctctg
     6301 ggacaaaatc catgaaccgg cagagttaat actggttcta tttcccaatc gtacctacca
     6361 ttaggatttt taccataggg atctgctcca ccttgtatca gctctaattc ttcatttgtt
     6421 agatttatat tttttgtcat taaaatcatg atatatttcc tccattattc ttcagattag
     6481 ttgagtaatc tgatactcat acctacttac aaaaaaacta ttatattaag ttggtttttt
     6541 aatttgtcta gttgcaacat tgacaaacat agcatagcat atctttgcga aatatcctct
     6601 tcaaatcatg aattgtcatc aaaacatctt aaactataaa atcaattagt ctcaagcttt
     6661 ctatcaattt cttctccaaa atatgctata ataatagcaa aagataaaga aggaagacct
     6721 atgattaaac tactagcctt ggatatggac ggcaccctcc tcaatgaagc caaggaaatc
     6781 ccacaagctc acattactgc tattcacaaa gccattgaaa aaggtgtcaa actggttctc
     6841 tgtacgggtc gccccctttt cggtgtcctc ccctactaca aaaaactgga gcttgacctc
     6901 cagaacgaat atattattgt taacaacggt tgttcaactc accagactag cgactggggc
     6961 ttggttgact ggcaagaact gagtccagct gacatcgaat acctctatga ccttgctgaa
     7021 aagagtgatg ttcagttgac actttttgac gagtcacatt attttgttct cggtggcaaa
     7081 cccaatcaag ttattgaaaa tgatgctaaa ctagtatttt cagacctgac tgaaatttct
     7141 cttgaagaag cgactagtgg aaagttccgg atgttccaag gtatgttttt aggaacaaaa
     7201 gaacaaacag acgattttga gcagcgtttt gctgaagagc tttgccaacg attcagtgga
     7261 gttcgttcgc agcctgtcat ttatgaagca atgccgcttg gaacgacaaa ggctactgct
     7321 ctttcacgac tagctgagat tttgaagatt gattcctcag agattatggc catgggcgat
     7381 gctaataacg atatcgaaat gctccagttt gcagggcttg ggattgcaat gggaaatgcc
     7441 agcgattatg tcaaatctct tgcggatgcc gttacatcaa gcaacgaaga agacggcgtt
     7501 gcgcgtgcta ttgagaaata tattctataa aaaagaaaag caaatagaca gaagttactg
     7561 ctatttgctt ttttgctaat attttaaaat acggactaat caatagacaa gaatagcgaa
     7621 tcataaaaat cgatttacta gatgccatag tatcttatag ttgctaataa gaagttagtc
     7681 tagcagaatg aatctctcat ttctcccaca ctaatctgta atttgtctat acatttctgg
     7741 tcttctatct cgaaacaagc cccagtttag gcgttcactt gctcccttgt ctaggtcata
     7801 agtagctaac agaacagctt cttcttgtct ttcagctcgt tctagaatag ctcctgtttc
     7861 atccgtcata aaggaggaac cgtagaagtc aagactggaa ctctgtccgc cattttcctc
     7921 actaggagta acctcctcta aaccataacg attggctgcg atgactggaa caatattcgc
     7981 tgctgcgtgc ccttgcatag tacgttgcca gtgaccacaa ctatctgtat ccaaaattgg
     8041 ctctgaaccg atagctgtag gataaaagag caattcagca ccattcaatg caagacagcg
     8101 cgctgtttca gggaaccatt gatcccaaca gataccgata ccaatcttag catagcgagt
     8161 attccagacc ttgaaaccag tgttaccagg cgtgaaatag aatttttctt gataataatg
     8221 gtcatctggt atatgggtct ttcgataaac gcccagcact tccccatctg catcaatgac
     8281 ggcaatagag ttatacaaga cattaccatc tttttcatag aaactgattg gtaaaacaac
     8341 ttgtagttcc ttagcaatca ccttaaaatg ctgaatggca gtattttccg ctacagattg
     8401 ggcatactgg tagtagtcat actgacgttc ctgacagaaa tagggatgtt caaacaactc
     8461 gggcaagaga ataatttggg ctccttgctc agcagcctga cgtactaaac gctctgcggt
     8521 ttggatattt gttgccacat ccttagcgca ttgcatctga atggttgcaa ctcttacatt
     8581 tctcatcttt ttctcctatt ctggaatttg ttgggtgata cagtggatat tgccaccacc
     8641 taagagaata tctctggctg gtattccgac aactttacgg tctgggaaac acttgctgag
     8701 gatatctaag gccacttggt cgtttacatc ctcaaactgt ggaaccaaga cagccttgtt
     8761 ggcgatataa aagtttacgt aggaagctgc tagtcgttca cctgcgtatc gcttttcttc
     8821 tccttcttca tagatgtagc ctggcaaatc ttcttctgtc acaacttgtc gaactgcagg
     8881 gataggcaat ttatgaatgg tgaagtgaca accttttgca tctgtttcct gttctaagag
     8941 ttcgagatct gcttttgaca tggcatactg gggatcattt tcgtcatctg tccaagccaa
     9001 aacaagctca gcaggaccaa caaaggcagc aacattatcg acgtgttcat tggtttcatc
     9061 ctgataaata ccataaggaa gccaaataac tttttcagca ccaagacttt ctaataatgt
     9121 gttttcaatc tcttctttag tcaggttagg attgcgacca ggactaagca agcaactttc
     9181 agttacgaga atagttcctt gaccatcgct atggattgcg cctccttcca gtacaaaagg
     9241 tttagcatca tagacaggcc tttccaaggc ctcagcaaaa cgactggcta cttggtcatc
     9301 ctcttcataa tcttgataaa gaccatcata ggtgcctccc caagcattaa aggcccaatc
     9361 cacggctaat ttcttacctt tatcagtgac gagaatggtt gggccagtat cacgcgccca
     9421 ggcatcattg gtgggaatgt ctaaataaac aaccttgtct ccaagatagg attgggcttc
     9481 agatagatag gcctgctcca ccaaaagata gactctttcc ccttctgcta tggtctcgat
     9541 gatctgagta aatgctcttt tagcagcctt tccttgaaaa ggccatgatc ctggtcgagt
     9601 cggccatatc atgagggtac catgatgggg ttcgtactct gctggcatgt gatagcctaa
     9661 tttttttgga ctgtccatca tgataatctc cctttaaagt cttgatagcc aaaagctttg
     9721 agtaagctac agtctccctg ttcgtccatg agatagagac ttggcaatcc aataccatta
     9781 aaggtattat ttttgacaaa agaataaatg gccatgtctt gaaaataaag tctgtctccg
     9841 atttggactg gattttcaaa actataatca ccaatcacat cgcccgtcag acaggtatta
     9901 gaagaaagtc tgtaggtatg ggctttttcc tgtgcctcaa agccatttct caaaggtgga
     9961 cgatagggca tctcaagtac atcaggcata tggcaggtcg cagaggcgtc taaaaccaag
    10021 atttccatac cgttttctac aatatctaat acctcagttg ctaaataacc cgcattaagc
    10081 gcaatggctt caccaggctc gatatagatt tcaagattgt aagtttttcg gatacgcttg
    10141 atttctgaaa tcagcaaatc cacatcgtaa ccttctcttg taatatgatg accaccaccc
    10201 atattgagcc attttacctc atgtaagtag ggaccaaact gttcttctac tgctttcaaa
    10261 gttgtttgta aatcatctgc tccctgctcg caaagggtat gaaaatgaag tccgtcaacc
    10321 aaatccagca aatcactcgg aatcttgtct atagtaactc caaagcgaga acctggtgca
    10381 caagggtcat agagcgcgtg atctccttga gttgaacact gagggttgag gcgcaaacca
    10441 acactgacac cagcctctcg acaacgcgga ccgtgtttac gcaactgtct ctctgagtta
    10501 aagacgatat ggtccgttat ctctagcaat tcctccaagt ctgcatcctt gaaagcaggc
    10561 gcaaatacat ggacttcacc aggaaattct tcccttgcca atttggcctc atagagtcca
    10621 ctagctgtcg tacctgatag atactggcta atcaagggat aagttttgta gagggaatat
    10681 gccttctggg caagcaagac cttgcaaccg gcctcttctt gtacatattg tagaatgcgg
    10741 caattagctt ctaacttggc caagtcaata acataggctg gtgttggtac ttgttctaac
    10801 ttcattagtc caccatttgt ggattttcaa ccacaaccca tggcaaacca tactcattca
    10861 aagcttccat gaatggatct ggatctaact cctcaaggtt atacactcca gcttgtttcc
    10921 aagttccgtt catgactaat tttgtcccaa tcatggctgg aactcctgtc gtgtatgaaa
    10981 ttgcttgtga cccgacttct gcatagcact cttgatggtc gcaaacattg tagatgtaga
    11041 tagtcttttc aacgccatct ttgacacctg taaagataca tccaatattg gtttttccga
    11101 ctgtacgtgg cccaagactg gcaggggctg gaagcaaggc ttttaagaat tgaatcggaa
    11161 cgatgtcttg tccattgaag ttaatggtat ccgtacgaag gagtcctaca ttttcaagac
    11221 atttcatatg tgtcaagtaa gattgaccaa aagtcataaa gaaacgaatg cgtttgactc
    11281 caggaatgtt cttggccagc gattcaattt cttcatggtg aaggagatac atatcttttt
    11341 gtccaacttg agggaaatcg tactcacgtt tgatagacat agcttcgact tcgacccatt
    11401 gtccatcttc ccaataagaa cctggcgcag aaacctcacg gagattaatt tctgggttga
    11461 agttggttgc aaatggataa ccgtggtcac cgccattaca gtctaaaatg tcgatataat
    11521 ggatttcatc aaaatagtgt ttgagggcat aagctgaaaa gacactagtt acacctgggt
    11581 caaaaccaga accaagaaga gcagtcaagc ctgcttcttt gaatttctct tgataagccc
    11641 actgccatga gtagtcaaag taggctgtaa aaccaagttc cttacaacgt ttttcgtaga
    11701 tagcacgcca ctcagggtct tctgtgtctt ctgcttcgta gttggctgta tcgatatagt
    11761 gaacacctgt tgccaaacaa gcatccataa tggttaaatc ttgataaggc agagctacat
    11821 tcaaaacagc ttctggtttg tagctttcaa tcagggcaat cacttcttca accttgtcag
    11881 catcaagagc agctgtctca atctttgtac ttgttttgcc ttccagttta gctttcaagt
    11941 catcacattt tgacttggta cggctagcaa tcataatctc tgtaaatgtt tcgctatctt
    12001 gacaaatctt cgaaatagca acttgggcaa cgcccccaca accaataact agtaaacgac
    12061 tcattttttt ccttcctctt cttctaaaat gtcttctaca tacttgggca acataaaggc
    12121 tcctacatgt aagtttgcag tgtagtattt tgtgaaaagc tggcgttttt tccagccttc
    12181 cttgtcaaaa tctttgacag gatggtattt tttcgatgca aatccaaata accaatagcc
    12241 cgccggactg gtagggatat gggcttggta gacacgacta attggaaagg tctgattgac
    12301 ctttcggtgc atgcttcggc aagccgactc atcctcatca aagaagggac tcccatgctg
    12361 gtaaatcata atgccgtctt ccttcagagc tcgataacta ttaccgtaga attccttagt
    12421 aaagagccct tccgtatagc caaagggatc tgtcgcatcg ttgatgataa tatcataatc
    12481 atcttcgcag tttcgcaaaa aacgtagccc attttgataa taaatggtaa cacgtggatc
    12541 atctagccct gcagcaaagt ctggaaaata ctcacgacaa acctcaacca acatttcatc
    12601 aggttctaca atatcgattt gttccaattc tggataaagt gttaatactt gagcaacacc
    12661 accgtcacca cccccaataa ccaagacttt cttgggattt gggtggacag tcatgggaac
    12721 atgaacggtc atttcattgt agacaaagtc atccgcatct gaaaataaga cgtgcccatt
    12781 taaaatcagt attttcccaa aagctggtgt atccaagact tcgatatctt gccattcact
    12841 ttttccagcg tagagttgct tggctgttct cagggacaat tttacatctg gagtatgaac
    12901 ttcagaaaac cataaatcca tctagttctc ctttctctta atgacgttga tgtgattgac
    12961 ctcaggatct tccgtccctt ggagggaaca accacgttcc ttggcaaatt ggatatagtc
    13021 tacaatttct cgtgtaatgc gttcaccagg agccaagata ggaatccctg gaggatagca
    13081 catgacaaat tctccacaga cttgtccaac agattcgtcc aaggttaaac tttttctctc
    13141 tgaatagaag gcttcctgag gagataacac caactcgggc tgaatatatt ctcctgctat
    13201 caagtccttc ccatctcgtg aatagagtct ctttatgtca gccaaagcac cgaccaagcg
    13261 ctcgatgtct tggatgcggt caccaatcga aatataggcc aagatattgc caatatcacc
    13321 aaactcaatc tgaatgtcgt attcgtctcg taggaagtca taaacctcga tacctgttaa
    13381 gccaataccc tgagtgtaaa ctgacagctt ggttacatca aaatcacaga ccgacacacc
    13441 atctattaac tcttttgagt aggcatagta tccaccaata gcattgattt cacgacgagc
    13501 gtactcggat agctcaatga ccttctcaaa cgactcttta ccacgaaggg ccaagttgcg
    13561 acgtgaaata tccaaactag ccatcaacag gtaagaagca gatgttgatt gggtcaggtt
    13621 gatgatctga cgaacgtact cgggattcat ctgctctccg attaaaagaa gcgaactttg
    13681 tgtcaaactc ccaccagact tatgcatgga gactgctgct atatcagcac ctgcatccat
    13741 agcagaaatt ggaagtttat ctgtaaaatg caaatgcgct ccgtgggctt catctactaa
    13801 aaccatcata ccagcttcat gagccatttc tgtcaacccc tttaggtctg aacagattcc
    13861 gtagtaagta ggattgttaa tcaaaatggc cttggcatct ggatggtctt ttatggcctg
    13921 tgctactcga acattttcaa gacctaaagc gataccaatc ttaggatcca cactcatctc
    13981 gatatagatg ggaatggcac cacatagaac cagcgcattg atagcagatt tatggacatt
    14041 tcgtggcaga ataatcttat ctcctgcctt acaggttgac agaatcatag tctgcaccga
    14101 tgaggttgtt ccccctatca ttagaaaagc atggctagct ccaaaagcat ctgcagccag
    14161 ctcctctgca tcacgaataa tcgaaatagg atggcctaaa ttatccaagg gtttcatcga
    14221 attgacatca atgcctacac atttttctcc taagagttcg acaagttctg gatttcctcg
    14281 tccacgcttg tgacctggaa catcaaaggg aacaatcctt ttcttgcgta acttcaccaa
    14341 ggcctcataa attggggctt ggttttgatc taactccttc aagacatact cctttcaata
    14401 tttttaggac cttatgaaaa ctaaggaact aaaggttgac tcatgaaaaa agagcaggtt
    14461 ttcacctgct ctgcaatctt ctgacgtgtt tattttggtt tctgttgagt atgtctgttc
    14521 ccgatgtttc ctaactctct agtagcaaat attacgtact ttattgatcg tttcacaaga
    14581 tgacgtgtgc tttcaccaca ctaaaaattt atgaattagg acaagtgtcc taacatcaac
    14641 ttataaaagt aggcttttaa ccctatcaat aatgtggctt ttcaaccacg cttcggcatt
    14701 caaccctgcc tgtccgtagg cattttttac tcgggtttat atttgctaga aaacatcatc
    14761 tcattttcta agctttatta ctatatcatg tttttaagaa cttgtaaagc ggtttacgaa
    14821 aaaaatataa aaaagttcgc aaaatatttg gattttattt gatttaa
//
LOCUS       DAVKDZ010000046        14521 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000046 DAVKDZ010000000
VERSION     DAVKDZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14521
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.047"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(232..1839)
                     /locus_tag="VWZ30_001412"
     CDS             complement(232..1839)
                     /locus_tag="VWZ30_001412"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU9335131.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKIFLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2229..2816)
                     /locus_tag="VWZ30_001413"
     CDS             complement(2229..2816)
                     /locus_tag="VWZ30_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEU9335132.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2944..3393)
                     /locus_tag="VWZ30_001414"
     CDS             complement(2944..3393)
                     /locus_tag="VWZ30_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9335133.1"
                     /translation="MCFVPYYKVNYCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(3398..3787)
                     /locus_tag="VWZ30_001415"
     CDS             complement(3398..3787)
                     /locus_tag="VWZ30_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEU9335134.1"
                     /translation="MKVNIVDLHPTQLYLSEKKLQDIQMLYQSAETNQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3815..4465)
                     /locus_tag="VWZ30_001416"
     CDS             complement(3815..4465)
                     /locus_tag="VWZ30_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9335135.1"
                     /translation="MKDKQTFLMKGSFALLLFVILGYMVKFYPETLVGFDQSIQTAIR
                     GDLPDYLTILFRSLTHLIDIPVIITWVVIIAFVFYRKRWKIESFFMLGNLALAGLLIV
                     TFKNIYQRPRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQI
                     VLGLYLVSVLVSRVYLGVHYPSDVLASFCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4455..5018)
                     /locus_tag="VWZ30_001417"
     CDS             complement(4455..5018)
                     /locus_tag="VWZ30_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9335136.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5183..5401)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5455..5958)
                     /locus_tag="VWZ30_001418"
     CDS             complement(5455..5958)
                     /locus_tag="VWZ30_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU9335137.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFMSRMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6151..6981)
                     /locus_tag="VWZ30_001419"
     CDS             complement(6151..6981)
                     /locus_tag="VWZ30_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9335138.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVASLLLILQNQGRFYNPWK
                     "
     gene            complement(6966..8648)
                     /locus_tag="VWZ30_001420"
     CDS             complement(6966..8648)
                     /locus_tag="VWZ30_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9335139.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLSLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQVQLQTELVKETSELQSLFRLEEVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(8750..9007)
                     /locus_tag="VWZ30_001421"
     CDS             complement(8750..9007)
                     /locus_tag="VWZ30_001421"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335140.1"
                     /translation="MKIKEKTRKQVAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(9014..9562)
                     /locus_tag="VWZ30_001422"
     CDS             complement(9014..9562)
                     /locus_tag="VWZ30_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEU9335141.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVINS
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9577..10425)
                     /locus_tag="VWZ30_001423"
     CDS             complement(9577..10425)
                     /locus_tag="VWZ30_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEU9335142.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGYITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQLVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10614..11963
                     /gene="trkA"
                     /locus_tag="VWZ30_001424"
     CDS             10614..11963
                     /gene="trkA"
                     /locus_tag="VWZ30_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEU9335143.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGGDFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            11967..13406
                     /locus_tag="VWZ30_001425"
     CDS             11967..13406
                     /locus_tag="VWZ30_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9335144.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSVIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYIALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRVKAFFGDEELRAYL
                     VIVLVSTGLITLNTLYLYPGFSKSFEMAFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMTIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLSLIFIVSLDSNDFLIVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            13492..14292
                     /locus_tag="VWZ30_001426"
                     /pseudo
     CDS             13492..14292
                     /locus_tag="VWZ30_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         4305 a         3259 c         2703 g         4254 t
ORIGIN      
        1 aatccagttc ctaaaacttt gctagaaatt gatttggctt tccgaattaa tttgttcaaa
       61 ttttatttca tttcaatata ctgataaatc cattcactag cacagggcaa gagggagtcg
      121 ctattgattt caagggtagc aggtacatac agaggactcc tacctcagat atactgcaat
      181 atcatcgtat gcaaaaaacc tctgagattt ttctcaaagg ttctgatttt gctaattgct
      241 gttctcaacc gctgcagtaa caaaggcagt gtagagttct tctggtcggt ttggacggct
      301 tgacagttca gggtgatact gacaagctac aaagaattta ttttcaggaa tttccacgat
      361 ttctaccaaa cgattgtctg gagaaactcc tgaaaagaca aaacctgctg cctcaaactg
      421 ctcacggaag gcattattaa actcataacg gtgacggtga cggcgttgca ccacttcttg
      481 attgtgataa gcagcagcag ccttagagcc acgtttcaac ttagacggat aaagtcccaa
      541 acgaagggtt ccacccatat cctcaatatc aatctgatca cgcatgatat caatgatagg
      601 gtattttgtt tctggtgcaa gctctgcaga attggcacct tcaagaccta aaacgtgacg
      661 agcaaactcg atacatgtca actgcattcc caagcagact cccaacattg gaacatcatt
      721 ttcacgcgca tagcggatgg cttggatttt cccttctgta ccacgttgac caaaaccacc
      781 tggtacgatg atcccgtccg catcagacaa gagttctgct acattctctg ctgtcacatc
      841 attggcattg acccaattga ttttaacttc tacatcattg acatagccag agtgtttcaa
      901 ggcttcgacc actgagatat aggcatcttg caactccaca tacttaccaa caaggaaaat
      961 cttaacttgt ttcttgaggt tcatgacctt gtccaccatg gctgaccatt ctgtcatatc
     1021 cgctgctggt gcgtctaatt tcaaatgatc acaaacaatt tggtccatcc cttgtgcctg
     1081 caagttcagt ggaatttggt aaaggtgttc aacatccaac gattcgataa cggcttctgg
     1141 tgccacatca cagaactggg ccagtttatt tttaattcct tgaccagctg gctcttctgt
     1201 acgaataacc aacatatttg gttggattcc caagccacgc aattctttga cagagtgttg
     1261 ggttggtttg gttttcattt caccagcagc cttgaggtaa ggaagcaagg ttgtatggat
     1321 atacatgaca ttatccgcac ccacatctgc cttcatctga cgaagagcct ctaggaatgg
     1381 caaggactcg atatctccta ctgttccacc aacctctgtg ataatgacat cagagtcggt
     1441 cgttagagcg gcacgcttga ttttttcttt caaagcatct gtgatatgag gaatgacttg
     1501 aacagttgcc ccaaggtatt ctccacggcg ttctttacga agaacttcac tgtaaatttt
     1561 cccagttgtc acgttggaat atttgttgag attgatatcg atgaaacgtt catagtgacc
     1621 caagtccaaa tctgtctcag ctccgtcatc tgtcacaaaa acttccccgt gctggtaagg
     1681 actcatggtt cccggatcaa tattgatata agggtcaaac ttttgaatgg ttactttgag
     1741 accacgattt ttcaagagac ggcctagact cgctgccaca atccctttcc caatagacga
     1801 taccacacca ccagttacaa aaatatattt cgtagacata gattcctctt tctaaaatgc
     1861 tcaaggtctt gttaactacg aggggatata gaaaacaaga ccctactaat aagaataatc
     1921 tgggacgact gcaccagatt cacaactaat actcttcgaa aatctcttca aaccgcgtca
     1981 acgtcgcctt gccgtagata tgtgttcctg actttgtcag tcttatctac aacctcaaaa
     2041 cagtgttttg agcagcctac ggctagtttc ctagcttgct ctttgatttt cattgagtat
     2101 aaaatacaag aagataactt cttgaaaaaa caaaaatagc tccctaagaa ctagggagcc
     2161 ccgacctcta aaagaggtgc ccaaacaata tgatacctaa aaataggata attgtcaata
     2221 gctaactctt attcctcgct ggtttcagcg tcgtcatctg aaaactcgtc gtcgtcttcg
     2281 ttgagatcca catcttctcc caagtcatct ggagcgattt cgttgatttc tgcatcataa
     2341 gcttccactt catttttttc atcatctgga ttttcatcat cgtatgaaag agctggatct
     2401 gcttcgtatg catcttcgtc ttctggatca tctgcattgt agtcaatggc atctgaatca
     2461 ccatccataa aggcattgac acgttttttc ttagcttttg gtgctacttc atcgtcgtca
     2521 ttttcttcaa gagcgatgat ttcttcgtcg atttcgtcca caccatacca tgaacgaaga
     2581 ccccatttgt tgtccccaag tgagatgaag ctaccgtcaa agttcaactc tgtgtagaac
     2641 aaaggcaaag cttcgcggat atcgctgttt gatgttccaa ggtagttttg aatttcgttt
     2701 acaagatcgc taaaatgcat ctcatgatcg cgaccacgaa gttccaagat agcacgcgct
     2761 acctcaatca tagatagttc acttttttct tgcccagcaa atacttctaa ttccaaagcg
     2821 tttctcctca tttatactac tatcgccaga gcgaacagac tctgacctca ttttatcatt
     2881 tactctttat tttacgataa ttttgcggaa tagtcaaagg ttaaggggga gaaagtggca
     2941 ggattagact aattccaata taaaactcct tcctttttct gttgctccat tttccacaaa
     3001 tccaagcgac ttgaaacacc tcctagaagc atgattgtag gtgtagattt ccttgactct
     3061 caattctttc catcctttta ctcgagccaa ttcaatcaaa gcacttagaa tcttttttcc
     3121 aagtcctcga tgttggtaag cggaattccc aatcacaatg gggagattat cctgagatag
     3181 tgtaatatcc ccaattggaa accattctcc cttctccttg acttcaatcc aaaaaagctc
     3241 accatgccga tccaaatagg aatacatggc ttccaaggtc gcttgactgt aaggaagctt
     3301 caccccatct acgaggtaaa ccaagttcac atcctgatac caagcaaaag cttcctcaca
     3361 ataattgacc ttataataag gaacaaaaca caatgcacta ccttttcaac aaaagagttg
     3421 ctgcttgatt aaaaccatca caccagttat accattttgc ttcataccca tcttgagcta
     3481 ggatacgatc ttctaaatca aaaacagagt aaatctttct ttcctcgcaa gcttgcgcat
     3541 agagatgata tagttcatca ccaccatctc tatcccactc agcagaaatc gtatcccgac
     3601 ctgccaataa agcctgataa gccctgtgat gcccatctgt aatcagcaaa caatctccaa
     3661 aggcaagaat actgattgga tcgacttggt ttgtttctgc cgactggtaa agcatctgaa
     3721 tatcttgcaa cttcttttct gataaatata gttgagtcgg atgaagatct actatattga
     3781 ctttcatttc tttctcctca agggaattcg atactcactt ctgtttgcct ttaaatcgcc
     3841 attggaagcg gagcttgtca tagaagggaa actcgataaa caagactccc aaacccacac
     3901 agaaactggc aagaacatct gatgggtagt gaactcccag atagactctt gataccagca
     3961 cactgactag gtagaggcca aggacgattt gtacgatttt tctccagacc tgatctttaa
     4021 tccgctgact aagaataaca atcaaagtcc ctaccatcag cgttacagct agagaatgcc
     4081 cacttgggaa ggaaaatccc ttctcctcca ccagatgtaa gatagctggt cgtgggcgct
     4141 ggtagatatt tttaaaggtc acgattaaaa gacctgccaa agccagattt cccagcatga
     4201 agaaactttc tatcttccat cgcttacgat aaaagacaaa agctataatg acaacccaag
     4261 tgataatcac tggaatatca atcagatgtg tgagggatcg aaaaagaata gtcaaataat
     4321 ctggtaagtc tcctcgaatg gcagtctgaa tcgattggtc aaaaccgacc agcgtttcag
     4381 ggtaaaattt gaccatgtag ccaagaataa cgaaaagtaa aagggcaaaa ctgcccttca
     4441 ttaaaaatgt ttgtttatct ttcatagtgt tttaaggttg gtttcaaaag gacgtacaac
     4501 aaccagaatg aaacggaaaa gattacaccc tcaatcaagt taaaaggtaa taccatggtc
     4561 attaggtagt tggaaagtcc caaaattttt ccaatatcaa agttagcaaa cttagcgtac
     4621 aaaggaacag cataaacata gttgagaacc aacatggcca aggttaaacc aacagttcca
     4681 gctagagagc ctagtaggaa acgaagggtt gtccgttcct ttttccaaat caaagcaaat
     4741 acgatgacaa aaactcccaa agctacgata ttcatcggca aaccaatgta agtattcact
     4801 ccttggctgt taagaagcaa tttcaatagt gagcgaagca agagcactcc tagagcagca
     4861 ggcaaatcca tgaccaccag acccacaagg actggcaaga tactaaattc gatcttgagg
     4921 aaagatgccg ctggtaaaag cggaaagtca aagtacatca gcacaaatga gatggctgat
     4981 agaattgcaa tggtcgaaag tcgacgtgtg tttgtcataa caggttcctc caattttcta
     5041 taaaatcaga agaagttgga aaggattcct ctatctattc tcacttttta tatcccaaaa
     5101 gttccctctt actctattaa agaaaaacaa agcaagtggt tacaatccgg ctataaatct
     5161 atcaaaacag acaaagctat tctttcgtct tctcccatcc agactatact gtcggttgtg
     5221 gaatctcacc acatcagctt gcgctcgcgg acttattttg ctaagatatt ttagatttat
     5281 ctaggcgtcg cagtcgaaga taatacttaa ggtatatcga gacaaaacaa cgacgagaaa
     5341 gctaaaatag ctagtcaaat ttaccgccgg tcgggaattt caccctgccc tgaagactct
     5401 tttatcataa caaaaaacgc ttgcaagcgc aagcattttg ttcattttca ttttttattt
     5461 caatttatcc acttcatagg tatggacgag ctcaagacct gcaaagttct gctggcgaag
     5521 agcctcatag acaatcatgc agacggtatt agacacattg agactgcgga catgttcatc
     5581 attcatggga atacgaagag ccttctcagg atgttctcgc ataaaatctt caggtaagcc
     5641 cttgtcttca cgtccaaaga gaaaataatg gtcctcgtca gtcgataaat ccacctcaga
     5701 atagactttt tccgcgaatt tcgaaatcag atagagtttt cccttcatcc gagacatgaa
     5761 atcttccaaa ctctcgtaaa aataaatctc tagcttatcc cagtaatcca atccagcccg
     5821 cttcatcttg cggtcatcaa taggaaagcc cataggcttg atgatgtgga ggggagaatt
     5881 ggtcgcagcg caagtacgcg cgatattgcc cgtattttgt ggaatttgag gttcaaataa
     5941 tacaatgtga tttgtcatga cttgcttcct ttcaccattg caaaaaaata gccacactgc
     6001 ccggagtcaa gctcagcaaa cagcgtggtt aaggcatcgt taacttacct cacaacaggt
     6061 ttgaagtaaa tcagcgaaac tactttctta gtataacact ttcagaatca ttgtcaatag
     6121 aaacgacttg attttttaaa ttttttcaag ctatttccaa gggttgtaaa atcgtccctg
     6181 attctgcaag ataagtagta aactagctac taaaaacaag gttgccaaga gcaaggtaat
     6241 atagtctcct tttttcaagg cctgataact ataccatgtg cgttttttct ctttcccaaa
     6301 gcggcgaagc tccatggcag tcgcaatggt atcaatgcgt tctagcgagc taaaaatcaa
     6361 gggcgtaata atgagcagat tgcctttgat tcgttgcata agagaagctt tcttggataa
     6421 ttccatccca cgcgcctcct gagacatctt gatagtaaag aattcttcct gcaaatctgg
     6481 aatatagcgc aaggtcaggc tgacagaata agcaatctta tagggcacac caatttgatt
     6541 taaactggaa gcaaactgac taggatgggt tgtcatcaaa aagataatag ccagaggaat
     6601 ggtgcaaaga tacttaatgg ccaaatttag cagataaaag agctcctggc tggttagagt
     6661 gtagacaccg attccctgcc aaatcacact tctctctcca taaagtccaa ccccatactc
     6721 gggagaaaag agatagacca tcaaaacgtt taaaacggca aataccgtcg caaaaacggc
     6781 tacaaaggag acatctttaa agcggatttc tgataaatag aggagaaaga ctgaaaagat
     6841 ggcaatcagc aagagcattc tggtatcata gctaatcatg gccgccaatg ataccagaat
     6901 gaaaaagaga agtttcccag ctcctgacaa gcgatgaatc acagtatctc tatgctggta
     6961 accgattaat ttagcttgca tccctctctc ctttctttgt aaaatgccgt taaatccagt
     7021 ggatccacat ctagtttctt agccaagtta aagatggagg tttcttttag attggctttt
     7081 actaacagct caggatcgct caacagactg gctggaacag tatcggcaat caattctcca
     7141 tccaccatga caaggacccg gtctgaataa tccagcatca attgcatatc atgggtaatc
     7201 atgacaatgg tatgcccttt ttgatgtaac tcttcgagaa attccataat ctcagtatag
     7261 ttcttctgat cttgacctgc agtcggttca tctaggagaa taatttcagc tcctaagacc
     7321 aaaattgaag caatggtgac acgttttttc tgaccaaatg acagggcaga aataggccaa
     7381 ttacggaatt cataaagtcc acagattttc aaggtttcat agactctcgt ttcaatttcc
     7441 ttctcatcca cacctcgcaa acggagccct agagccacct catcaaaaat catattggtt
     7501 gaaatcattt gattaggatt ttgtagcaca tatcctactc gttccgcccg ctctgcaaca
     7561 gaatcgcctt ttatatcctg tttttcccaa agatagcgtc cttccgtctg aataaagcta
     7621 cttatagcct tggctagagt tgatttccct gctccatttt ttccgacaat agcaatcttt
     7681 tcaccctttt taatatctaa atgtagggat tttaaaatcg gtctatcatc ataagaaaaa
     7741 gatacttcct ctagtctaaa gagtgactgc aattctgagg tttcttttac cagttccgtc
     7801 tgtaactgga cttgcccttt tgagatagac aagttatcca gatctgctaa ttgttcttcc
     7861 ttgactaagt ccacacctaa ttgacggaga gtggttagat aaaggggttc tcgaattcca
     7921 ttttgagtca ataaatcagt cgcaagcaac tggtcagggc tcccattaaa aaggatacga
     7981 ccatcgttta tcaagacaat ccgatccaca gggcgatgca gaacgtcctc caaacggtgc
     8041 tcgataatca gagtcgtcgt cccctcttcc ttatgaatct ggtcaatcaa ttcgataata
     8101 tcctgacctg acttgggatc tagattggcg agtggctcat caaacaagag aatcggactt
     8161 tcatcaatca agacaccagc cagactgact cgctgctttt gtccacctga caaatcctga
     8221 ggacgctgat ccagtaaaga aagaaggtcc aacttttcag cccatttata aacacgacct
     8281 ttcatctcat ctagggctgt cacatcattt tccagagcaa acgccaaatc ttctgccaca
     8341 gacaagccaa taaactgccc atctgtatcc tgcaaaactg tgctaaccag atgagactta
     8401 tcatagatgc tcatatcaaa ggctacttga ccctttatca aaaattctcc atatgtctga
     8461 cccttgtaaa tattgggaat aatcccattc aaacactgac ccaaggtaga tttacctgac
     8521 ccagatggtc caacaattaa gactttctct cccttgtaaa tggtcaagtc tatcccttgc
     8581 aaggtcggtt cttgttgtgt ttcataccgg aaagagaaat ccttccactc aattatagct
     8641 tctttcatct tactctcttc attcgcttct tagacttcta ttttatcata aatcaagccc
     8701 ttcttgcagt ctctcctctt aaaatcttag cgccaaaaag attcctatcc tagcttactt
     8761 gcctaactaa tctataaaca tcgaaaaaga ctagttgccc agccttcccc atcattttat
     8821 actcttcgaa aatctcttca aaccacgtca gcttcgcctt gccgtaggta tggttactga
     8881 cttcgtcagt ttcatctaca acctcaaaac catgttttga gctgacttcg tcagttctat
     8941 ccacaatttc aaaacactgt tttgagcaac ctgcgacttg cttcctagtt ttctctttga
     9001 ttttcattga gtattagtcc tttttcaaac ttcctgcacg agtttgggtt cctgcatagg
     9061 caagtaagag aagagttcct gcaatagcta cagatacacc attggcaatt cccgcaacaa
     9121 tcccttgtgc aaatactttt tctgccgctt cttgataaat cacaacatct ccaagtggtg
     9181 ccaagacacc ccaaacaagg gcatttgcaa gtagttgaat gagattaaaa ataagaatat
     9241 ctttccagtc aaaaacacta ttgatcacgc gaacgtactt tctaaaaagt cccacaacta
     9301 aaccaaagag tccgctagcg ataatccaag tccaccatag accataacca acaagagagt
     9361 ccttgattgc atgaccaatc aacccgacaa gcaaaccgat aatcggtcca aaaataatag
     9421 aaagtagcgc ttgtaccgca tactgaagct ggatgcttgt atttggaaca ggggttggaa
     9481 tgttgatcat cccgatgacg acaaagaggg cagcgccaat tccgacagca acaacttgtt
     9541 taattgtaaa tttgatttcc atactattct cctattttat ccttctattt tctttatttc
     9601 aatggtccaa gatgaaccga cacctacatt ataggccttg gcaaaggaac cttggttgat
     9661 agccaaacct aaacgataga gagagttgat gtaaaggatg ggttgcccaa ttctcacatc
     9721 tgcaaatgat ttgccataga caagctgatt ttgatagacc agcatatcag catgatagat
     9781 ggtcacttca aaacgatcac caaattctgg ttccagcttg taaaattctt cccgtgtgat
     9841 agaggtccaa agcgaaccga aacgcacatc cagaatatca atggctccct tcaccagatg
     9901 atcttctatg atggtcgcta cgactggaag ctctacaatc tgttccacac tgagctctgg
     9961 ccctacttcc tcaaaagtaa tgtaaccact ggccagttta gcaccagtat aggcatagac
    10021 atcacgaccg tggaaggtat aagaatgctc tgtgttttga cgcctattgg ccacctcaga
    10081 aatctcacga atggctacaa tgccaacgtg tttcttgata aaggaaagcg tcccattatc
    10141 tggcgtgaca atgtattgat tttttgcagt cttggcaact acactcttac gtttcgaacc
    10201 gacacctgga tcgacaaccg atacaaacgt cgttccctca ggccagtaat ccaccgtctg
    10261 aaagagacga tagctcccct caaaaatatt ataaggcgtg atatcgtgcg tcaagtgatg
    10321 gatttttaag gttggagact cttctaaagc cactccaatc atagccgata ccgcaccatc
    10381 aaccagacca aagtctgatt gtaataccag taaattatta ttcattttat ctccttgtat
    10441 atcctttatc ataccatatt tcagagaaag tcgctaatag ctatttcaat tggttagggt
    10501 atagttttac cttatagcaa atttcctact aaaagttctt gttattagct agtaggtgca
    10561 ttttttcttg aaaaagggta tgatagttac atcatgaaaa agtaggtttt attatgaaaa
    10621 ttatccttgt cggaggggga aaagttggtt ttgctctctg tcgctccttg gttgcagaaa
    10681 agcatgatgt tttgctgatt gagcaagacg aagctgttct caatcatatt gtcagtcgct
    10741 ttgatatcat tggtatcctt ggtaacgggg gcgattttgc cattcttgag caagccagcg
    10801 tccaagattg tgatatcttt atcgccctga ctgagcacga tgaagtcaac atgattgcag
    10861 cagttctagc caagaaaatg ggagctaaag aaactatcgt tcgggtgcgg aaccctgaat
    10921 attctaactc ttatttcaag gaaaagaata ttctcggttt ttctcttatc gttaatcctg
    10981 agctcttggc tgcccgcgct atcgcgaata tcattgactt ccccaacgcc ctgtctgtcg
    11041 aacgctttgc tggtggacgc gttagcctca tggaatttgt cgtcaagtcc accagcggtc
    11101 tttgccaaat gcccatttct gattttcgta aaaaatttgg taatgtcatt gtctgtgcga
    11161 tagagaggga tcatcaaatt atcattccaa gcggtgacat gactgtacag gataaagata
    11221 gaatctttgt cactggtaac cgtgtcgata tgatactctt ccataattat tttaaatcac
    11281 gcgccgtgaa gagccttctc atcgttgggg caggtagaat tgcctattat ctacttggta
    11341 ttctcaaaga tagtcgtatc gatacaaaag tcattgaaat caatcctgaa atcgccagct
    11401 tctttagcga gaaattccca aatctctaca tcgttcaagg agatggaacc gcaaaagata
    11461 tcctgctgga agaaagtgct caacactatg atgccgttgc gactctaaca ggagtcgatg
    11521 aggaaaatct gattacatct atgttccttg acagggtagg tgtacagaaa aatattacta
    11581 aggtcaatcg taccagtctc ctcgagatta tcaatgcgcc tgatttttca agtatcatca
    11641 cacctaaaag catcgctgta gatacgatta tgcactttat tcgtggtcga gttaatgccc
    11701 agtattcaga ccttcaagcc atgcaccatc tagccaatgg ccaaattgaa accctgcaat
    11761 tccatatcaa ggaagccaat aaaatgactg ccaaacctct ttctcaactg aaattgaaaa
    11821 aaggggttct tattgcagcc attattcgaa agggcaagac tattttccca actggggagg
    11881 atatgttgga agttggagac aagctcctag taacaacctt gttgccaaac atcaccaaga
    11941 tttatgactt gattgcgagg taagaaatga ataaaagtat gattcgttac ctcctttcaa
    12001 agttactttt gattgaagct gttctcctct tggttcctgt ggctatcgct gtctattacc
    12061 gtgaatcgag ccaagtcttt acagccctct tttcgaccat agggattctc gtattgttag
    12121 gcggttcagg gagtttacag aagccaaaaa atcaacggat ttatgccaag gagggagtct
    12181 tgatcgttgc cctctgttgg atcctttggt ctttctttgg tggtctcccc tttgtctttt
    12241 ctgggcaaat ccctagcgtt attgatgcct tttttgaaat tagttctggg tttacaacta
    12301 ctggagcaag tattttgaac gacatttcgg ttctcagccg ttccctcctc ttctggcgaa
    12361 gttttaccca cttgattgga gggatgggag tgcttgtttt tgcacttgct attatggaca
    12421 atgccaaaaa tagtcaccta gaagtaatga aggctgaggt tcctggtcct gtctttggta
    12481 aggttgtatc caaattaaaa aacactgccc agatcctcta tctcctttac atagccctct
    12541 tttccctctt tgtcatcatc tattatctag ccggtatgcc tctctacgat agttttgtca
    12601 ttgctatggg gacagcggga actggaggat ttaccgtcta taacgatgga attgcccact
    12661 atggtagctc actgattacc tatctggtta gtataggagt tttggttttt ggggtaaatt
    12721 tcaatctcta ctactacctt atgctccgtc gggttaaggc cttctttgga gatgaagagc
    12781 ttcgggctta cttggtcatc gtgctggttt ctacaggctt gattaccctc aatactctct
    12841 acctctaccc aggattttcc aaaagctttg aaatggcctt cttccaggtt tccaacatca
    12901 ttacaacaac tggttttgga tatggagata ttaccaactg gcccctcttc tcccagttta
    12961 tcctcctctt cctcatgaca atcggtggtt ctgctggatc aactgcaggt ggactcaaga
    13021 ttattcgagg cctcatcctt tcaaaaattg ccaaaaatca aattttgtcc attctatctc
    13081 cccaccgtgt tttgactctc catgtgaata aaacggtgat tgataaggat acccagcata
    13141 aaattctcaa gtactttgtc atctatgcta tgattttgct atcccttatc tttattgtca
    13201 gcctagatag caatgatttt ctgatcgtga ccagcgctgt ctttagctgt ttcaataata
    13261 tcgggcctat tctaggaacc acttctagtt tctcaatctt tagtcctatc tcaaaaattc
    13321 tcctctcctt tgcaatgatt gcaggccgct tggagattta cccaatccta cttctcttta
    13381 tgaagagaac ttggtctaag agataaaata caaaatctct ctttttacaa ttatgaaaga
    13441 gggattctta tattatactt cctgcggaac aaaatatggt atagtagttc tatgaattat
    13501 gaagcaagta aacaactaac tgatgcacga ttcaagcgtc ttgttggtgt tcagcgcatg
    13561 acttttaaag agatgttagc tgtattaaaa cagcttatca acttaaacac gcaaaaggtg
    13621 gacgaaaacc taaattaagc ctagaagacc ttcttatggt cactcttcaa tatgtgcgag
    13681 agtatcgaac ttatgaagaa attgcggctg attttggtat ccacgaaagc aacttaatcc
    13741 gtcggagcca atgggttgaa gtaactcttg ttcaaagtgg tgttacgatt tcaagaactc
    13801 ctctcagttc tgaggacacg gtaatgattg atgcgacgga agtacaaatc aatcgcccta
    13861 aaaaaagaat tagcgaatga ttctggtaaa aagaaatttc acgctatgaa ggctcaagcg
    13921 attgtcacaa gtcaagggag aattgtttct ttggatatca ctgtgaacta ttgtcatgat
    13981 atgaagttgt tcaaaatgag tcgcagaaat atcggacaag ctggtaaaat cttggctgac
    14041 agtgattatc aagggctcat gaagatatat cctcaagcac aaactccacg taaatccagc
    14101 aaactcaagc cgctaacagc tgaagataaa gcctataacc atgcgctatc taaggagaga
    14161 agcaaggttg agaacatctt tgccaaagta aaaacgttta aaatgttttc aataacctat
    14221 cgaaatcatc gtaaacgctt cggattacgg atgaatttga ttgctgatat tatcaatcat
    14281 gaactaggat tctggttttg caggaagtct aatatgaaaa caagacgact tccatatagg
    14341 aaaccgcctt ctagctatgt tgagtgattt atattaaaat aacttttctt ctagcttcat
    14401 tttattatta taaaaacatt catcataacc cccagaactt aaataacgat ttttattcaa
    14461 gatacatact cctagaataa actttatatg aaatccccat ttttgttttt acaattctcc
    14521 t
//
LOCUS       DAVKDZ010000047        14297 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000047 DAVKDZ010000000
VERSION     DAVKDZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14297
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.048"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..251
                     /locus_tag="VWZ30_001427"
     CDS             <1..251
                     /locus_tag="VWZ30_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9335145.1"
                     /translation="SSSKKGIQASMSRKGNSQDNGRMESFFGILKSEMFYGYEKTFKS
                     LNQLEQAIIDYIDDYNNKRIKVKLKGLSPVQYRTKSFG"
     gene            complement(294..2144)
                     /locus_tag="VWZ30_001428"
     CDS             complement(294..2144)
                     /locus_tag="VWZ30_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9335146.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTIHIE"
     gene            complement(2186..3343)
                     /locus_tag="VWZ30_001429"
     CDS             complement(2186..3343)
                     /locus_tag="VWZ30_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9335147.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYLNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKGTIIYLDKISQINQNML"
     gene            3401..4567
                     /locus_tag="VWZ30_001430"
     CDS             3401..4567
                     /locus_tag="VWZ30_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEU9335148.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVMLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQSSSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIDILMTVALFTVDRADKIL"
     gene            4706..5032
                     /locus_tag="VWZ30_001431"
     CDS             4706..5032
                     /locus_tag="VWZ30_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9335149.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            5019..5612
                     /locus_tag="VWZ30_001432"
     CDS             5019..5612
                     /locus_tag="VWZ30_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEU9335150.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEVAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKRKRGNQHA"
     gene            5605..6516
                     /locus_tag="VWZ30_001433"
     CDS             5605..6516
                     /locus_tag="VWZ30_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEU9335151.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILQLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTDLTDSQLESTEKHFHAEN
                     IQVHGKVELTAKKYLRIILDQKENQRINWDVSSNYGSIFQFTREEPESRGTELSNPYK
                     TKKADVKDQLIARSDDDIELISTPSRR"
     gene            6578..7642
                     /locus_tag="VWZ30_001434"
     CDS             6578..7642
                     /locus_tag="VWZ30_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335152.1"
                     /translation="MKQEWFESNDFVKTTSKNKPDEQAQDGADKAEETIADLDTPIEK
                     NTQVEEEVSQAEAELESQQEEKIETPEDSEARTEIEEKKASNSTEEEPDLPKATEKVT
                     IAEESQEALPQQKSTTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSSSSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEEEWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(7841..8676)
                     /locus_tag="VWZ30_001435"
                     /pseudo
     CDS             complement(7841..8676)
                     /locus_tag="VWZ30_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
     gene            complement(8951..9937)
                     /locus_tag="VWZ30_001436"
     CDS             complement(8951..9937)
                     /locus_tag="VWZ30_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU9335153.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(10209..11684)
                     /locus_tag="VWZ30_001437"
     CDS             complement(10209..11684)
                     /locus_tag="VWZ30_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEU9335154.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(11942..12865)
                     /locus_tag="VWZ30_001438"
     CDS             complement(11942..12865)
                     /locus_tag="VWZ30_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9335155.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(12879..13808)
                     /locus_tag="VWZ30_001439"
     CDS             complement(12879..13808)
                     /locus_tag="VWZ30_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9335156.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
BASE COUNT         4286 a         3084 c         2634 g         4293 t
ORIGIN      
        1 agagttcgtc aaaaaaggga attcaagcat ctatgtcacg caagggaaac agccaagaca
       61 acggtaggat ggaatctttc tttggcattt taaaatccga aatgttttat ggctatgaga
      121 aaacatttaa atcacttaat caattggaac aagccattat agactatatt gatgattaca
      181 acaataagag aattaaggta aagctaaaag gacttagccc tgtgcaatac agaactaaat
      241 ccttcggata aattaattgt ctaacttttt ggggtcagta cattttttct tttttattca
      301 atgtggattg tttgattagc aaaggcgata atagcttgct tcaactcatc tccactgaga
      361 gtgcggaact ctccaatctt ttgattgatg gattctaaag gcatgacatt aaccttacca
      421 acgaaaatct tatccataac ttgattgtca acgagttcgg ttgataccaa gagttcttcg
      481 tagagttttt cacctgggcg gattccaact tcaacgattg gaatttcact ttcagtatgg
      541 ccacttagaa gcaccatctt cttagccaag tcataaatct tgactggttt gcccatatca
      601 aggataaaga cttccccatc tttggcataa gcaccagcat ggataaccag acggctagct
      661 tctggaatgg tcataaagta acgggtcatg cggaagtctg tcaccgttac aggcccacct
      721 tcagcaatct gacgttcaaa gactggaatg acactaccac ggctaccaag aacattccca
      781 aaacgaactg cacagtaggt tgattggcta cgttggttaa agccagtgac aatcaactcc
      841 gccacgcgct tggttgctcc cataacattt ggtggattaa ctgccttatc tgtcgaaatc
      901 ataaccatct tagacacttt agcttcatca acagccttag caacattgta agttccacgg
      961 atattgtttt tgaaggcttc ttttggattg cgctccatca taggaacatg cttgtgggct
     1021 gccgcatgat aaacaatagc aggtttgtac tgctcaaaga cttgcaacaa acgatcatag
     1081 tcttgaatgt ccgcaatcac aggtacataa tcaatccctt ggaacttacg aatcaattca
     1141 tgataaacaa ggtagattga gttttcccca tgaccgagca agacaatgcg ttcaggattg
     1201 aagcgactaa cttgacgaca gatttcagaa ccgattgaac ctccagctcc tgtgactaag
     1261 atggtcttac ctgtcagttc tgcacccaga cgcgattcgt caagacggat ttcctgacga
     1321 cccaaaaggt ccgtaatatc aattttttgg aagccagtac ctgcttggtg aaggccctga
     1381 acaacagttt caaccttagg catcttgtaa catttgacac ccagcttatt acacatctgc
     1441 aagatacgct catattctga cggatccagc gacggaatcg caacgatgac acgctcgatt
     1501 tgatggcgtt tggctaattc aggcagattg tcataagagc ccaaaacagg aataccacca
     1561 agtttttgac cctttttctt agaatcctta tccaaaatac cgaccagttc taattcactg
     1621 gttggatgtt ggtaactatc cataaagaga gccccaccat caccggcacc aatcaagaag
     1681 gtccgacggt gttctccatc accactacct tttttgcgtc tggagtagat taactgccaa
     1741 gtaatccgtg gcaataaaat caagaaggta ctcaacaaga taaagagaat gatgaaacgg
     1801 atggagaaga gtggcaagaa ggcataacag atactatatg acaagacact gctagcagtc
     1861 acaccaaaaa agattttcat gaaatccgta atcttgctgt aacgactaat gctcgcgttc
     1921 aacccccaaa aaccaatcat caattgatag aacaggaagg ccaaactcgt atagataatg
     1981 tagtcaacag gtgctggatt aatcagtcca taaaataaaa tataagatac aatgatggaa
     2041 accaccatac tgaaaatatc aaatattccc cagaaaacct gcttttgttg tttatttaaa
     2101 atttccacca gatcaatcac ataatctgtt agttttttat tcatagaaat ctactcctcc
     2161 ttaaaagagt ttcactatat tgttattata gcatattttg atttatttgg cttattttat
     2221 ccaaataaat tattgttccc ttgtactcct tcttccattt aaatcctcta attaaaccta
     2281 caatcgtccc aaagccataa gcaaagtgaa tggaaaataa aagaaagggc atcacaatta
     2341 gaaatccatt tttatgtttt aataaagtca gcaaagtgag tagtgacaaa agtagaaaat
     2401 aggcacctaa tagtaaagtt atgaatacga atgtgatcgg taacaatgct agactaaaca
     2461 caagactcaa aacaaataaa caaggaacat agtgaaataa tgataaacac ttaaactgaa
     2521 catgacttgt caagccaatc cacaaaccat ttaaatactt ttgatgcagc attttcttga
     2581 atgttggtcg aatatactga taagatagaa tacttgggct atagcggatt ttataaccat
     2641 gttctcgaat tctataatga atatcattat cttcagttcg gccaagttgc tcatttacta
     2701 aaccaacctt ctggaaaacc tctcgtttat acatcccatg aaaaatagaa gaaacatatc
     2761 tatcctcgga actatttcga taattggcaa tgctactgcc aaacatattt tcctcaacaa
     2821 gatgcaaggt ctctgcccat tttccttttc cttcgacaat cgtcggtcta ggccccccac
     2881 agacaaattc accttgttga ataatagcca cattgttcat tacaaaactc tcagtaactt
     2941 ttgaatgagc atcaattttt aaaataaggt cccctacaga atgtttaact cccaggttaa
     3001 aaccactagc ttgatttttc ttaggattgt tatacaatct aattgagtta aactctgtat
     3061 cttcctttat aaattgctga atgatagctg tggtcccatc tgtggacata gcatttataa
     3121 atagaatttc aatattctct ttaggatagg tttgattttt taagtcttca attagaccag
     3181 gcagatattt ttcttcattg tatgcactga ttacaatgga tacgagaaga ctttcaacat
     3241 tttgagtcac tactactctc ctgaattttt ccttccacca ttctatcata attttcaaga
     3301 ctttcccatt tttatcttga aagccagaaa ccttacttga cattatagtg aaaaagcctt
     3361 aaaataagct gttattaaaa tcagctagaa gaggtattat atgaaaaagc aatcactctt
     3421 ttttgttcca ggtattatcc tgattggtgt ttccttgcga actcctttta ctgttttacc
     3481 cattattttg ggaaatattt cgcaaggtct ggaggtagaa gttagttcgc ttggtgtctt
     3541 gaccagcctg cctctcctta tgtttaccct cttttcacca ttttctaccc aactggctca
     3601 gaaaatcggc ttggagcatc tcttcaccta cagcctcttc ttcttgacca tcggctcact
     3661 tattcgacta atcaatctgc ccctgctcta tctaggaacc ttgatggttg gggcaagtgt
     3721 cgcagtcatc aatgtcatgc ttcctagtct tatccaagcc aatcaaccaa agaaaattgg
     3781 ttttctgacc accttatatg taacgtctat ggggattgca acggctctgg cttcctatct
     3841 agctgtgccc attacacaat ccagttcttg gaaaggactt atcctcctcc tcacattact
     3901 ctgtctagca acttttttgg tctggctccc aaatcaccgc tataatcata gactagctcc
     3961 acaaaccaaa caaaaaagtc aaataaaggt catgcgtaat aaacaggttt gggcaattat
     4021 tatcttttca ggttttcaat ccttgatctt ttacaccgtc atgacctggt tacctaccat
     4081 gtctatccat gcaggtctat ccagtcacga agctggcttg ctgacttcta tcttatctct
     4141 gattagcatt cctttttcaa tgaccatccc aagcctgaca accagtttat ctactcgcaa
     4201 ccgtcagctc atgctcactc tggtttcact agctggtgtg gtcggcattt ccatgctctt
     4261 tttcccaatc aataatttca tttactggct tgccatccat ctcctcatcg gaaccgcaac
     4321 cagcgccctc ttcccttatc tcatggtcaa cttttcactc aagacaagcg cccctgaaaa
     4381 gacagcccaa ttgtccggcc tatctcaaac aggaggctat atcctagcag cctttgggcc
     4441 aaccctcttt ggttacagtt ttgacctatt ccactcttgg gtaccagctg tagctgccct
     4501 cttgctcatc gatatcctga tgactgtagc cctctttaca gtggacagag cggataagat
     4561 cctttaaact tctagtttta gctagaagtt ttttatatat aaaaatttta cacatttctc
     4621 ttgacagggc tttcttttag atgtacaatg tatgtgtaga aaaattatat ataaaaatct
     4681 tacacattag aaaaggaggt ttcccatgta ctttccaaca tcctctgcct tgattgaatt
     4741 tctcatcttg gctgtactgg agcagggtga ttcttatggt tatgagatta gccaaaccat
     4801 taagctgatc gctaatatca aagaatccac actctatccc attctcaaaa aattggaagg
     4861 caatagcttt ctgacaacct attctagaga gttccaaggt cgcatgcgca aatactactc
     4921 cttgacaaac ggtggtatag agcagctctt gaccctaaaa gatgaatgga cactctatac
     4981 agacaccatc aatggcatca tagaagggag tatccgccat gacaagaact gaatacctga
     5041 ctcagctaga actctatctc aagaaactac ctgaagctga ccgtatcgaa gccatggact
     5101 atttcagaga gctctttgac gatgctggag tcgaaggaga agaagaactc atcgctagtt
     5161 tgggaactcc caaagaagtg gcccacgaag ttctatccaa tcttctcgat aaaaaaatca
     5221 atgaagcacc cgctcaaaaa aataaccgac aaattttaca tatcgccttg ttagccctcc
     5281 ttgcagcacc tatcggcatt cctctgggaa tcgccatcct cgtgaccctg ttcgcaatcc
     5341 ttgtagccgc tttgactgtc attctggctt tctttgcagt ttccatactg ggtatcatcg
     5401 gcggattcct atttttagtt gaaagtttca ctgtcctcgc ccaagccaaa tcagccttta
     5461 tcttgatttt tggttctggt ttactggcta tcggtgcttc ttcgctagtt ttactaggca
     5521 tttcctatgt agctcgcttc ttcggtctac tcattgttcg cctggtacaa tttgttctta
     5581 aaaaaagaaa gagaggtaac cagcatgcgt aaatggacaa aaggatttct catctttggt
     5641 gtggtgacta ccgttatcgg ctttatcctg ctttttgtag gtatccaatc tgacgggatt
     5701 aagagcctac tttccatgtc caaagaacct gtctatgata gccgtacgga aaagctaacc
     5761 tttggcaagg aagtcgaaaa cctagaaatt actctccacc aacacgcgct caccatcaca
     5821 gactctttcg atgatcaaat ccacatttct taccatccat ctctttctgc tcaccatgat
     5881 cttatcacca atcagaacga tagaactctg agtctcactg ataagaaact gtctgaaact
     5941 ccgtttctct cttctggaat tggtgggatt cttcatatcg caagtagcta ctctagtcgt
     6001 tttgaagaag ttattctcca gctaccaaaa gggagaactc taaaagggat caacatctca
     6061 gccaatcgcg gacaaaccac catcataaat gctagccttg aaaatgcgac cctcaataca
     6121 aacagctata tcctccgaat tgaaggaagt cgtatcaaaa acagtaaact cacaacccct
     6181 aatatcgtca atatctttga tacagacctt acagatagtc agctagagtc aacagagaag
     6241 cacttccacg ctgaaaatat ccaagtccat ggtaaggttg aactgactgc caaaaagtat
     6301 ctcagaatca tcctagacca gaaagagaac caacgaatta attgggatgt ctcaagtaac
     6361 tatgggtcta tcttccaatt cacaagagaa gagcctgaat caagaggtac ggaattaagc
     6421 aacccttaca aaactaaaaa agccgatgtc aaggatcaac tcattgcgag atctgatgat
     6481 gatattgagc taatatctac accaagcaga cgttgacaaa aacgcttaca tctgctacta
     6541 tggaagcata tttatctaac aaaaaggagg ttctaccatg aaacaagaat ggtttgaaag
     6601 taatgatttt gtaaaaacaa caagcaagaa caagcctgat gaacaagccc aagatggtgc
     6661 agacaaggct gaagaaacga tagccgatct cgatacgcca attgaaaaaa atactcaagt
     6721 agaggaggaa gtctctcaag ctgaagccga actagaaagc cagcaagaag agaaaattga
     6781 aactcctgaa gacagtgaag cgagaacaga aatagaagaa aagaaggcat ctaattctac
     6841 tgaagaagag ccagaccttc ctaaagcaac agaaaaagtc actatagctg aagagagcca
     6901 agaagctctt cctcagcaaa aatcaactac gaaagagcca cttcttatca gtaaatcctt
     6961 agaaagtccc tatatccccg accaagctcc aaaatctagg gataaatgga aagagcaagt
     7021 acttgatttt tggtcttggc tagtggaagc gatcaaatct cctacaagca agcttgaaac
     7081 aagtatcaca cacagctaca cagcctttct cttactcatt ctattttctt catcttcctt
     7141 tttctttagt atataccaca tcaaacatgc ttactatgga catatagcaa gcattaacag
     7201 tcgcttccct gagcagctag ctcctttaac tcttttttct atcatctcta tcctagtagc
     7261 gacaacactc ttcttctttt cattcctctt gggtagtttc gttgtgagac gatttatcca
     7321 ccaggaagag gaatggacgc tagacaaggt tctccaacaa tatagtcaac tcttggcaat
     7381 tccaatcttc ctcactgcta ttgctagttt ctttgctttc tttgatagcc tacgatttac
     7441 agccctcttg tgtgtgatta gcattggaat cattctactt gccagtctcc atatcattac
     7501 aagaccaagt caagcaagtg aaaccgactc cttctatcag ttattcttat ctgtccttgt
     7561 gaacggagtt attatcctcc tcttctttgt agctgaagtg gcactgattg gagattatct
     7621 tcgtatcttg gcctttcttt aaacttcata ctcttcgaaa atctcttcaa accacgtcag
     7681 cttcgcctta ccgtactcaa gtacagcctg cggctagctt cctagtttgc tctttgattt
     7741 tcattgagta gcaatcctgt cttccatggc aggatttttt ctatgtcaaa aaagcaagtc
     7801 gattgacctg cttctgcttt acttttcttg tgctaatgtt ttaaaatctt tgtcctagga
     7861 tgggttgtat ttctatttga ttggcgtcta gttggtggta aaaagatggg ggtaatacct
     7921 ctaagaattt tgcatagtcc aagcgggcta tggcttcata gtcttctggt ttagagccgt
     7981 ctcctgtgat ttgaaccaag tcaatctccc actgcacttc cttatccacc aattgctcaa
     8041 tataggctgc aggaacaaac ggttctctcg gtaaaactgt cttgactcct tgagctagat
     8101 ggtaaacgcc atgcactttt ccttctccat cctgatagaa ggaaactctc gcaaagtagg
     8161 catctgtctc ttgaaccgca cacgcgcttc aaactgagcc aagccatctg cctctaaaaa
     8221 acttttcaag ttctcacgac taaagaaaat aggattaaaa attccttggc aaatcgtaaa
     8281 acgagctaca gggtctgcta gataggcttg aatacttgct tggaaataag cttcaaaatc
     8341 aaaaccatct agcccttcaa tatcagtccc tgtataagca agcaaagcat ctaaaaatga
     8401 cttgataatc tgccagtctg tctttgttag taggtcagga agatcgacac ggtaagcctt
     8461 ttgaccatca gcataactga ccttaaagag aaattgtgat tgccccacta tcgcacactc
     8521 gatatattcg agacgattaa gaggctgacg gagatagact acatcatagc tatgtgactc
     8581 caaaccgtct agcaaggcca agatcgactt ggcattcaaa atctcttgct ctcctaaaat
     8641 gctctgttta tttggaataa aaaatgtttt cgccataatt ggaccccttt aaaatcgttt
     8701 acatacagta taccttattt ttatgaaaga tacaaaaaca aactttatac tcttcgaaaa
     8761 tctcttcaaa ccgcgtcagc ttcgccttgc cgtagatata tgttcctgac ttcgtcagtt
     8821 tcatctacaa cctcaaaaca gtgttttgag caacctgcgg ctagcttcct agtttgctct
     8881 ttgattttca ttgagtatta gatttttgag tacaaagcat agaaaaacag cccctgagga
     8941 ctgtttgatc ttatacagta gctgcttgat ccaagctttc accgatagcg gctaggcgct
     9001 cgatcacttc agcttgtgtc aattcatttt ttgaaacata gcggttacgt gggtgaacac
     9061 ggcactcgtg tgagcatcca cgaaggtact tgtcttcatt ttcttctgat gtcaagatac
     9121 gacggttaca gaagggattt ccacagttga cataacgttc acatggtgtt ccatcaaacc
     9181 agtctttccc tacgatggtt gggttgacat ggttgacatc aacggcgata cgctcgtcaa
     9241 agacgtacat tttcccatcc caaagctcac cttgaacttc tgggtcttta ccgtaagttg
     9301 caattcctcc gtgcaattgg ccaacatctt tgtaaccttc acgaaccatc cagcctgaga
     9361 atttctcaca gcgaacacca cctgtacagt aaaccacgac acgcttgtcc atgaattttt
     9421 ctttgttgtc acgaacccat tgtggcaact cacggaagtt gcgaatatct gggcgaatag
     9481 ccccacggaa atgtcctagg tcgtactcat aatcgttacg tgtgtcaagg acaacggtat
     9541 ctttatcaag aagcgcttct ttgaactctt ttggagacaa gtaagcacct gttgtttcaa
     9601 gtgggttgat gtcattgtca aagtcgttgt cttccaaacc aaggtggaca atttctttct
     9661 tgtagcgaac aaacatcttc ttgaaggctt gttcattttc ttcgtcaatc ttgaaccaga
     9721 gttcttccat tcctggaagg ctgtgaacgt agtccatgta tttttgagtt gtttcatagt
     9781 cacctgaaac tgttccgtta attccctcgt cagcgactag gatacggcct ttaagaccga
     9841 ttgatttaca gaaagccaag tggtctgcag caaattgctc tgcattttca attggagtat
     9901 aaaggtagta aagtaagaca cgaatatctt ttgccataag atttgttctc ttttctattc
     9961 ttaaattttc taaatttttt atttatctac actagtatac ctgcttgaga aaacgaatgc
    10021 aatttatttg actggaaatt gtagagggtt ctcaaaattt cctgaatggt taaaataagg
    10081 acaagagaaa acatggatat ctatatcctt gtgccaaaaa aaccactgcc ctccccagac
    10141 caacctgagg aaagcagtga ttcttatttt aggagttagg aatgaataca cgaaatcaat
    10201 ttagctgatt attttttgtt tttcaagaat tcatcgtatt gtttttgcat ttcattcaat
    10261 actttttcgt aggcaccttc agatttcaac ttttccatca attctggaat cgctttatct
    10321 gggtctacag taccagtgtt gatagctgta tcaaattgtt gcattgtgtt agcaatagct
    10381 gagatttcag atttcacatt gtcagtattg aagataaatc caagcgctgg agattcttta
    10441 gcttctgcca attctttctt agaattttcg atttgttggt ctgtaacgtt ttcgttgatg
    10501 taaaggatcc agttgttacc agtgttccat ccacccatgt gagtgtttcc tttgtagcca
    10561 tcaagaacgc gaacacggtt ttctttacct tcaatttttt cccagttctt gccttctgga
    10621 ccgtaaacaa gaccgttcaa gagttctgga ttcgtattca agaggttcaa gatttccatt
    10681 gatttttctt tgttcttaga gttgtttgag atgacaaagt tagcaacttg tgttgtttgg
    10741 tttttcttga tgaagttagt aattggtttg atttggatat ctttgttggc aacacgtgaa
    10801 agcaagctgt taccgtagtc agctggtcct actgtttctt cacgaacgaa ccaagtatct
    10861 tgttgaaggt caaaggaagt atcgcttgtt gcgacatctt ttggaatgta gccagcttca
    10921 tagaatttgt gaagagtctt caagtgttct ttgaaacgag gcacttcgta acggtttaca
    10981 actttagtag tatcgccttc aagatcaata acgaatggaa gcccgtttgc tactgggtag
    11041 tcaaagttat cagatgggat gaaaacttta ccaatagcaa atggtactac gtctggagct
    11101 ttttctttaa tttgtttcaa gactggctca agagtttcgt aagaagtaac acctgaaata
    11161 tcgataccat atttggcaag gagagttccg ttgaaggcaa agttttgaga tgatgcaacg
    11221 ttggctgcaa ctggaacagc gtaaatttta ccgtttacag tattaccctt gatgtaagct
    11281 gggtcaagtg ccttgtaaag gtctttacct tcttttttgt acaattctgt caagtcagca
    11341 taagcacctt tttgagcatt tacaatatag ttatctgcaa aggcgatatc atagttttca
    11401 ccagatgatg tgataactga cattttctta ccatagtcac cccagccaag gtattggata
    11461 tccaatttag caccaacttt ttcttcgatg attttgttgg catttgctaa caattcatcc
    11521 aagttatctg gtttgtcacc gatttggtac attttgataa caggtttgtc acctgaatca
    11581 gcagcttttt tgctgttacc tgtcaagttg ccacaagcag caagacctgc agccagagcg
    11641 actacactag cagatgcaaa agcatatttt ttccagtttt tcatgataaa aactcctttt
    11701 tttattttta aacttataaa caatgtaatg atcttatact caatgaaaat caaagagcaa
    11761 actagaaaac tagccgcagg ctgctcaaag cactgctttg aggttgtaga taagactgac
    11821 gaagtcagtt acatatatct acggcaaggc gacgttgacg cggtttgaat ttgattttcg
    11881 aagagtatta acttcacaca agggaagttg ggaactgaga aatgttattt ctcaataagc
    11941 actattcttt cacaccaccg atagtcaaac cttttacaaa gtagcgttgg aagaatggat
    12001 acaaaatcgc gattggaagg gttgctacca caaccatggc catacgacct gtttctttcg
    12061 gtagagcaac tcccagttga ccagtcaagc cgaccgcttt ggcaatgtag tccatatttt
    12121 gttggatttg catgagcaaa tattgcaatg gatacaagtt gtcactcttg atgtaaagaa
    12181 gggcgttgaa ccagtcattc cagaaaccaa gagctgtcaa aagcgtgatg gttgcgatac
    12241 ctggtagtga caatggcaaa cagatttgga agaaaatccg ggcctcactg gcaccatcga
    12301 tacgagccga ttcgagaatg gcttctggaa tggtcttctt gaagaaggaa cgcatcaaga
    12361 tgatattgaa tggtgagaga agcattggaa caatcaaggc ccaaacagtg tcaccaagct
    12421 gaagtacacg ggtcaccatg atataacctg gtaccaaacc agcgttgaac aacatactga
    12481 gaaggacgaa gatggtaaaa aatctgcgat acttaaaggt tgtccgtgaa atagcgtagg
    12541 cataggttgt tgtgataaag acatttgtca atgtcccaac tacggttaca aagacagaga
    12601 tgaagagggc ttgtaggatt ttatccttaa actgtgccaa aaactcaaaa ccgtctaagc
    12661 caaattggga tgggaagaag ctatagccgt attggaggag gcttttctcg tctgtcactg
    12721 aaataatgat aacgaataca aaaggtagga tacaagagag ggcaatcaaa cccgaaatga
    12781 tactgaagaa gatatctgct ttcttactga aggagtgaat gccgacatta tcaatttttt
    12841 cttttttaat tttctttttt gccatattct cctcctttct agaacaaagc tgagtttgga
    12901 tcgactcgtc ttgcaagcaa gtttgatagg ataaccagaa tcaaaccaac aacggattgg
    12961 taaagaccgg ctgctgcagc cataccgata tctgctgtct gagtcaaacc attaaagaca
    13021 tatacgtcca aaacgttggt tacattgtaa agctgaccag cattgtgtgg gatttgatag
    13081 aagagaccga agtctgcgcg gaagatattt ccgactgcaa ggatggtcaa tacagttaca
    13141 agcggagtca actgaggaat ggttacgttg cgaatacgtt gccacttgct agctccgtcc
    13201 actgtcgctg cttcgtagta ggttggatca attcccatga tcgtcgcata gtacatgaca
    13261 ctgctatatc caaagccttt ccaaatacct aggaaaagta ggagataggg ccagatgccc
    13321 aggtcagcgt aaaaattgac ttctttgaga ccaagacttt ccaatagatg attgaacacc
    13381 cctttatcaa tatttaggaa ggcatctgta aagaaactga tgataaccca agacaagaag
    13441 taagggaaca acatagaagt ttggaaaatc ttcaccattc tcttagaacg gagctcgctg
    13501 aggataatgg caatccctac agatacaact aaacctagaa agataaagcc aagattgtag
    13561 aggacagtat ttcgtgtgat aataaaggcg tctcttgaac taaataagaa tctgaaatta
    13621 tcgagtccga cccatttact atttatgata ctatctatga aaccattact ggtcatgtgg
    13681 tagtctttga aggcaaccac gttcccaaat actggaatgt aaaagaatag aatcaaccag
    13741 agtgcccctg gcaaaaccat caagagaaag atccagttgt ctctcaatgt ttttgaaaac
    13801 tttttcataa tttcctccct ttttattttg atatccatct aaaaattctt ttttagactt
    13861 ttgataacga ttacattatt agtatactcc tatttgcagg ttaggttaaa ctcctaatta
    13921 tagaaaaaac tccacaaatt atgtagcaga tttaaaactt tatcaccact atcaaacaaa
    13981 tgtcctaaat caattgttta ttttatctct attagcccag tgatggcgtc actctgttat
    14041 aagcatccaa caacggggta tactgaaaaa tctccagact agggaactca gcgatagttc
    14101 ctaatctgga gatttttaat atgttattag gcgtttgctt tcaacttagc aataacctct
    14161 ttaagattat caatcaactc tgcttcagta tgctcagagc ctttttcatc tgccaagaac
    14221 aaaactgctt tttgaagttc ttcttgagag ttttcaagga catccttatc tactgtttca
    14281 aggtttgagt ctgctta
//
LOCUS       DAVKDZ010000048        14233 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000048 DAVKDZ010000000
VERSION     DAVKDZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14233
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.049"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            275..1596
                     /locus_tag="VWZ30_001440"
                     /pseudo
     CDS             275..1596
                     /locus_tag="VWZ30_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397931.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISLre2 family transposase"
     misc_feature    1974..2105
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2121..2651
                     /gene="infC"
                     /locus_tag="VWZ30_001441"
     CDS             2121..2651
                     /gene="infC"
                     /locus_tag="VWZ30_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU9335157.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            2684..2884
                     /gene="rpmI"
                     /locus_tag="VWZ30_001442"
     CDS             2684..2884
                     /gene="rpmI"
                     /locus_tag="VWZ30_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU9335158.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            2936..3295
                     /gene="rplT"
                     /locus_tag="VWZ30_001443"
     CDS             2936..3295
                     /gene="rplT"
                     /locus_tag="VWZ30_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU9335159.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            3353..3733
                     /locus_tag="VWZ30_001444"
     CDS             3353..3733
                     /locus_tag="VWZ30_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9335160.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLESLHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            3983..4783
                     /locus_tag="VWZ30_001445"
     CDS             3983..4783
                     /locus_tag="VWZ30_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU9335161.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKVNKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHGRGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            4794..5732
                     /locus_tag="VWZ30_001446"
     CDS             4794..5732
                     /locus_tag="VWZ30_001446"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU9335162.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKTILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            5774..7807
                     /locus_tag="VWZ30_001447"
     CDS             5774..7807
                     /locus_tag="VWZ30_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEU9335163.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAADEWVFDTHYQSWFYIKADANYAENEWIKQGDDYFYLKSGGY
                     MTKSEWVEDKGAFYYLDQDGKMKRNAWVGSSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDRQYQSWFY
                     IKENGNHADKEWIFENGHYYYLKSGGYMAVNEWIWDKESWFYLKSDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKATNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKN"
     gene            complement(7868..9523)
                     /gene="pavA"
                     /locus_tag="VWZ30_001448"
     CDS             complement(7868..9523)
                     /gene="pavA"
                     /locus_tag="VWZ30_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEU9335164.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMILRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMRKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHRVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            9628..10125
                     /gene="ybeY"
                     /locus_tag="VWZ30_001449"
     CDS             9628..10125
                     /gene="ybeY"
                     /locus_tag="VWZ30_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU9335165.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMFEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            10106..10501
                     /locus_tag="VWZ30_001450"
     CDS             10106..10501
                     /locus_tag="VWZ30_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU9335166.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFALTGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            10518..11417
                     /gene="era"
                     /locus_tag="VWZ30_001451"
     CDS             10518..11417
                     /gene="era"
                     /locus_tag="VWZ30_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU9335167.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKATKVPVILVVNKIDKVHPDQLLSQIDDFRNQIDFKEIVPISALQGNNMSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDCMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
     gene            11466..12290
                     /gene="mutM"
                     /locus_tag="VWZ30_001452"
     CDS             11466..12290
                     /gene="mutM"
                     /locus_tag="VWZ30_001452"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU9335168.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LTSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKAGQACSRCGTVIEKFQLGGRGTHFCPNCQRRD"
     gene            12290..12895
                     /locus_tag="VWZ30_001453"
     CDS             12290..12895
                     /locus_tag="VWZ30_001453"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU9335169.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRKQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            12882..14081
                     /locus_tag="VWZ30_001454"
     CDS             12882..14081
                     /locus_tag="VWZ30_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEU9335170.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVSFMSIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNETALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            14081..14230
                     /gene="rpmG"
                     /locus_tag="VWZ30_001455"
     CDS             14081..14230
                     /gene="rpmG"
                     /locus_tag="VWZ30_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9335171.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
BASE COUNT         4303 a         2526 c         3210 g         4194 t
ORIGIN      
        1 tgttgtaaaa taccttctat tggttgcgag ataataatct tgacaaccgt actgagcctg
       61 gttcttatat caggctttta ttttaccttg aaatagaaac agttgaactt ctgatactca
      121 actaaatcac tttagtatct ttcaataata aatagggcgt tgattttaat ttaaagaaac
      181 agcctaattt attcttggaa caaaaataaa tttgatgact tgttttaaaa atcatacaat
      241 tttcataaga attgaaaggg aaatgaaaaa ttgtatgaaa caatttgatg aattgagatt
      301 tagtagccag gaacaagaaa agacaaaaca acatttttta cagcatattg aacagtatga
      361 tgagtcgata agctccagta tgagatcaaa aggctatcgt tgtaaaaatc aagctgaacg
      421 taccgtagct tttacatttg gagaagtgac cttttctaga aaacgttggt ataagaatgg
      481 tgaatgtaga ataccagttg atgaaatgtt aggattagaa aagaatgtac gtcattcaca
      541 ggaactattg tatcaagctg cggttctagc tacaaaacta ccctatcgtc aggtaggaga
      601 gacaattgag atggtttata atgtaactgt cactaaagat accgtcaata aggttgttaa
      661 attagccaat caattactag aagaaaagga tgattatcga ttttttaaag aagagaagaa
      721 gattgaaaaa ttaaagcaga tatcatctac atagagggag atggtgtata tgtgaaaaca
      781 tctgaagatg gttctgaaaa aaagaatatg gatttatcac attttgtagt tcatacaggt
      841 tctaaacaga ttggcccaaa tcgttttgag ctagaaaata aaaaagagct tatccgattg
      901 gataagcgct cagctcgtag agaattgttg gatttattgg aaaatgaata tgaaattaca
      961 aatgggacaa tactagttac taattctgac tgtggcaagg gttatacacc acatgtattt
     1021 actgaaatcg ctaaatcatt taatgttaag aggcatgagc atttttggga cgaatatcac
     1081 ttgaataaag aaattaaaga atttacaaag tcttatccga gagaattgag agaagactta
     1141 tttcaagcta ttaaagagca tgataagaaa ttattaaggc gtacactaga tactatagaa
     1201 tcattggtta gtagtgaaga gggagaggaa aaatttgaat attattcgag taggttaatg
     1261 aggaattttc agtatacgaa accagcggaa atgaggggac ttccaaaggc aggtattggg
     1321 attatggaga gccagcatag aaaaataacg tatagaatga agcatagagg gatgtattgg
     1381 acaagtgaag gtgggatagc cgtagcgaat atgatattgt tagagcgtga aggaaaatta
     1441 agagatttat tttttggaga atggaaggag gactatgatg agtatacgtc attggatcat
     1501 atcagtgcgg atagtgttcg acctaaaaat gactctaaag actacaaact tccccgttta
     1561 cataaaaata tagaaaagat atttaagatg aaataattta agtgctaatt tcaacgaaaa
     1621 cacaaatata aataagcaaa aatttctagt gagatttggg cttttttggc gtggaataat
     1681 tgacatttat cctgattttg gtataataga gatataaaaa acaacgaaaa aaggagttgg
     1741 aaatgagcct ccaactccgc agagtaacac aacccaaact tgtattactc aatcaactaa
     1801 aatgatagca taagaaaaaa tcattgtcaa gggctgagca tacaaaaatg tttagccctt
     1861 tcgtgtattt gcatttattt atatggaaat ctgggatttc tcaaaaatca cgacacatac
     1921 aaaataactt tttatcttga caagagctag aaaacttggt atcatataaa agttgagaaa
     1981 agcagaagtg agagcttctc gccttgtgac attaagttgc ctggccctac ggatgaaaag
     2041 tttctaagaa acgctatcat aacgtgcggg cttgtatatt tacaagtccg ctattgtttt
     2101 tctctaataa aacaaaagag gtgaaaacca tagcaaagca agacttattc atcaatgatg
     2161 agattcgtgt acgtgaagtt cgcttgattg gtcttgaagg agaacagcta ggtatcaagc
     2221 cactcagtga agcgcaagct ttggctgata acgctaatgt tgacctagta ttgattcaac
     2281 cccaagccaa accgcctgtt gcaaaaatta tggactacgg taagttcaaa tttgagtacc
     2341 agaagaagca aaaagaacaa cgtaaaaaac aaagcgttgt tactgtgaaa gaagttcgtc
     2401 taagtccaac aattgacaag ggtgactttg atacaaaact tcgtaatgca cgtaagttcc
     2461 ttgaaaaagg aaataaagtt aaggtatcta ttcgctttaa gggtcgtatg attacccata
     2521 aagagattgg tgcaaaagtt ttagccgagt ttgctgaagc aactcaagat attgccatca
     2581 tcgaacaacg tgctaaaatg gatggacgtc aaatgttcat gcaattggcg ccagcgactg
     2641 acaaaaaata attgtcagaa agttaaataa aggagaaaaa atcatgccaa aacaaaaaac
     2701 acaccgcgca tcagctaaac gtttcaaacg tacaggttct ggtggactta aacgtttccg
     2761 tgcttacact tctcaccgtt tccacggaaa aactaagaaa caacgtcgtc atcttcgtaa
     2821 agcatctatg gtgcattcag gagattacaa acgtatcaaa gcaatgctta ctcgcttgaa
     2881 ataatagctt gactgtaagt aaacaattct aggaaatatt tggaggaaat aaaacatggc
     2941 acgtgttaaa ggtggcgttg tatcacgcaa acgtcgtaaa cgtattctta aattagcaaa
     3001 aggttactat ggagctaaac acatcttgtt ccgtactgca aaagaacaag taatgaactc
     3061 ttactactat gcataccgtg accgtcgtca gaaaaaacgt gacttccgca aattgtggat
     3121 cactcgtatc aatgcggcag ctcgtatgaa cggactttca tactcacaat tgatgcatgg
     3181 tttgaaattg gctgagatcg aagttaaccg taaaatgctt gctgacttgg ctgttaacga
     3241 tgcagtagct ttcacagctc ttgcagatgc agctaaagca aaacttggta aataacaact
     3301 gatgagactg gaacaggatt gtccagtctt tttaaaaata aaggagaaag atatggcttc
     3361 aaaaatgcta cacacttgct tacgagtaga aaatcttgaa aaatcaatcg cattttatca
     3421 agatgctttt ggttttaaag aattgcgtcg cagagatttt ccagaccatg ccttcacgat
     3481 tgtctatcta ggtcttgagg gtgatgacta tgagttggag ttgacttata actacgatca
     3541 cggtccgtat gtggttggtg atgggtttgc ccatatcgcc ctcagtacac ctgatcttga
     3601 gtcacttcat caagagcaca gtgcaaaagg ctatgaagtg actgagccaa atggtctacc
     3661 aggaactaca ccaaactatt actttgtcaa agaccctgat ggatacaagg tcgaagtcat
     3721 tcgtgaaaaa taatttcgtg caagttaagt agaatattcg ttttctactt gacttttttt
     3781 agctgaaaga gtatactaat aaaaattcaa cctttaaggg gagtccagag agactcacaa
     3841 ggtgtcagat aaaagaatag tgcaattttc tagaggagac tttttgaatg tgctctcttg
     3901 tgttgtacga ttttaactga ggccttgcac tagcaaggtc ttttctttat ctggtcccct
     3961 taaaatttaa ggaggaaaag ttatgaatcc cacatgtaag aagcgtttgg gtgtcattcg
     4021 gttggaaacc atgaaggtgg ttgcacaaga ggaaatcgcg ccagcaatct ttgaattagt
     4081 cctagaagga gaaatggttg aagccatgcg agcaggccaa tttcttcatc tgcgtgtacc
     4141 ggacgatgcc catctcttac gtcgtcctat ttcaatttcg tctattgaca aggtaaacaa
     4201 gcagtgtcac ctcatttatc ggattgaggg agctgggact gcaatttttt caaccttaag
     4261 tcagggagac actcttgatg tgatggggcc tcagggaaat ggttttgact tgtctgacct
     4321 tgatgagcag aatcaggttc tccttgttgg tggtgggatt ggtgttccac ccttgcttga
     4381 ggtggccaag gaattgcatg gacgtggagt gaaagtagtg acagtcctcg gttttgctaa
     4441 taaggatgct gttattttga aaacggaatt ggctcagtat ggtcaggtct ttgtaacgac
     4501 agatgatggt tcttatggca tcaagggaaa tgtttccgtt gttatcaatg atttagatag
     4561 tcagtttgat gctgtttact cgtgtggggc tccaggaatg atgaagtata tcaatcaaac
     4621 ctttgatgat cacccaagag cctatctatc tctggaatct cgtatggctt gtgggatggg
     4681 agcttgctat gcctgtgttc taaaagtacc agaaagcgag acggtcagcc aacgcgtctg
     4741 tgaagatggt cctgttttcc gcacaggaac agttgtatta taaggagaaa attatgacta
     4801 caaatcgatt acaagtgtct ctacctggtt tggatttgaa aaatccgatt attccagcat
     4861 caggctgttt tggctttgga caagagtatg ccaagtacta tgatttagac cttttaggtt
     4921 ctattatgat caaggcgaca acccttgaac cacgttttgg gaatccaact ccaagagtgg
     4981 cagagacgcc tgctggtatg ctcaatgcaa ttggcttgca aaatcctggt ttagaggttg
     5041 ttttggctga aaagctacct tggctggaaa gagaatatcc aaatcttcct attattgcca
     5101 atgtagctgg tttttcaaaa caagagtatg cagctgtttc tcatgggatt tccaaggcaa
     5161 ctaatgtaaa agctatcgag ctcaatattt cttgtcccaa tgttgaccac tgtaatcatg
     5221 gacttttgat tggtcaagat ccagatttgg cttatgatgt ggtgaaagca gctgtggaag
     5281 cctcagaagt gccagtttat gtcaaattaa ccccgagtgt gaccgatatc gttactgtcg
     5341 caaaagctgc agaagatgcg ggagcaagtg gcttgaccat gatcaatact ctggttggaa
     5401 tgcgctttga cctcaaaacc agaaaaacaa tcttggctaa tggaacaggt ggaatgtcag
     5461 gtccagcagt ctttccagta gccctcaaac tcatccgcca agtagcccaa acaacagacc
     5521 tgcctatcat tggaatgggg ggagtggatt cggctgaagc tgccctagaa atgtatctgg
     5581 ctggggcatc tgctatcgga gttggaacag ctaactttac caatccttat gcctgccctg
     5641 acatcatcga aaatttacca aaagtcatgg ataaatacgg tattagcagt ctggaagaac
     5701 tccgtcagga agtaaaagag tctctgaggt aaactgcaat caatctgttc ttgatttttt
     5761 attagtttgt aatatgaatt taggagaatt ttggtacaat aaaataaata agaacagagg
     5821 aagaaggtta atgaagaaag taagatttat ttttttagct ctgctatttt tcttagctag
     5881 tccagagggt gcaatggcta gtgatggtac ttggcaagga aaacagtatc tgaaagaaga
     5941 tggcagtcaa gcagccgatg agtgggtttt tgatactcat tatcaatctt ggttctatat
     6001 aaaagcagat gctaactatg ctgaaaatga atggataaag caaggtgacg actattttta
     6061 cctcaaatct ggtggctata tgaccaaatc agaatgggta gaagacaagg gagcctttta
     6121 ttatcttgac caagatggaa agatgaaaag aaatgcttgg gtaggaagtt cctatgttgg
     6181 tgcaacaggt gccaaagtaa tagaagactg ggtctatgat tctcaatacg atgcttggtt
     6241 ttatatcaaa gcagatggac agcacgcaga gaaagaatgg ctccaaatta aagggaagga
     6301 ctattatttc aaatccggtg gttatctact gacaagtcag tggattaatc aagcttatgt
     6361 gaatgctagt ggtgccaaag tacagcaagg ttggcttttt gacagacaat accaatcttg
     6421 gttttacatc aaagaaaatg gaaaccatgc tgataaagaa tggattttcg agaatggtca
     6481 ctattattat ctaaaatccg gtggctacat ggcagtcaat gaatggattt gggataagga
     6541 atcttggttt tatctcaaat ctgatgggaa aatagctgaa aaagaatggg tctacgattc
     6601 tcatagtcaa gcttggtact acttcaaatc cggtggttac atgacagcca atgaatggat
     6661 ttgggataag gaatcttggt tttacctcaa gtctgatggc aaaatggctg aaaaagaatg
     6721 ggtctacgat tctcatagtc aagcctggta ctacttcaaa tctggtggct acatgacagc
     6781 caatgaatgg atttgggata aggaatcttg gttttatctc aaatctgatg ggaaaatagc
     6841 tgaaaaagaa tgggtctacg attctcatag tcaagcctgg tactacttca aatctggtgg
     6901 ctacatggcg aaaaatgaga cagtagatgg ttatcagctt ggaagcgatg gtaaatggct
     6961 tggaggaaaa gctacaaatg aaaatgctgc ttactatcaa gtagtgcctg ttacagccaa
     7021 tgtttatgat tcagatggtg aaaagctttc ctatatatcg caaggtagtg tcgtatggct
     7081 agataaggat agaaaaagtg atgacaagcg cttggctatt actatttctg gtttgtcagg
     7141 ctatatgaaa acagaagatt tacaagcgct agatgctagt aaggacttta tcccttatta
     7201 tgagagtgat ggccaccgtt tttatcacta tgtggctcag aatgctagta tcccagtagc
     7261 ttctcatctt tctgatatgg aagtaggcaa gaaatattat tcggcagatg gcctgcattt
     7321 tgatggtttt aagcttgaga atcccttcct tttcaaagat ttaacagagg ctacaaacta
     7381 cagtgctgaa gaattggata aggtatttag tttgctaaac attaataata gccttttgga
     7441 gaacaagggc gctactttta aggaagccga agaacattac catatcaatg ctctttatct
     7501 ccttgcccat agtgccctag aaagtaactg gggaagaagt aaaattgcca aagataagaa
     7561 taatttcttt ggcattacag cctatgatac gaccccttac ctttctgcta agacatttga
     7621 tgatgtggat aagggaattt taggtgcaac caagtggatt aaggaaaatt atatcgatag
     7681 gggaagaact ttccttggaa acaaggcttc tggtatgaat gtggaatatg cttcagaccc
     7741 ttattggggc gaaaaaattg ctagtgtgat gatgaaaatc aatgagaagc taggtggcaa
     7801 aaattagtac tataagtgaa tatgatttga gtgaatagta agttaaaaat cctgatttca
     7861 agtaaaatca ggattttttc atggatgcaa tttttttgga gtctggtgtg acgcggaggg
     7921 tcttttgtcc tgtgtaagtg acaaagccgg gttttccacc agttggttta ttgagttttt
     7981 tgacttcaat catatctacc tgcaccagat tcgacaggcg cccttgagag aagtaggcag
     8041 ctaactctgc tgcgtctgtc tttactgcat cagatgggtc aagatttcct gagatgacaa
     8101 catggcttcc aggaatgtcc ttagcatgga accaaagttc ctccttgcgg gccattttaa
     8161 aggtcaattc ctcattttga agattgtttc gtccgacata gatgatggtt ttgccatcgc
     8221 ttgctagata ttgttctagt tttttgcgtt tctggatttt ctcccgttgt cttctgcgga
     8281 taaaacctgt ttgaatcaat tcttcacgga tttcagcgat ttcttccagt ccagcttggt
     8341 tgaggacggt ttctacactt tccagataga gaatagtggc tttggtttct tcaatcaaat
     8401 cagtcaagta tttgacagct tctttgagtt tctgataccg tttaaaatag cgttgggcat
     8461 tctggttggg agtcagagcc ttatcaagcg caatcatgat aggttggttg gtatagtagt
     8521 tgtctaggat aacctgatct tggtcgttag gcactcggtg gaggaaggtt gtcagtaatt
     8581 ctcctttttg acgaaattct tcagcgttgt ctgtcgccag taactctttt tcctgttttt
     8641 tgagtttgtg tcggtttttc tgaagttcat tttcaacacg acgaatcagt tcactggcct
     8701 gctgtttgac gcggtcgcgc tcagccttat ccttatagta ggtgtccaac aaatcagaaa
     8761 gatttgcaaa aggctctccc acctgatttg caaaaggaac tggactgaag gaagtctcag
     8821 tcaagcatgg cttggtttct tgattgaaaa aatttcggaa agcggaaagt ttttcactaa
     8881 ccagtatcct ttccaattca tttgccgtat cgcgtcccag accttgaaag aggctttgaa
     8941 gattttttgc tgttagttct tgggtttgca ggatttcaaa gagcttttca tccttgatag
     9001 taaaaggatt gagagattct gtacttggcg gagcgatata ggtcgatcct ggaagtaagg
     9061 tgcggtagct attttgtgaa aagccgacgt gtttgataac ttcgaggatt ttatgactgc
     9121 ttttatcgac cagtagaata ttactgtgtt tcctcataat ttcgataatc aaggtagcct
     9181 ggatatggtc tccaatctcg tttttattgg aaactgtaat ttccacaata cggtcatttt
     9241 ccacttgctc aatcgactca atcagggccc cctgcaaata ctttctcaaa atcatgataa
     9301 aggtagaagg ttgggctgga ttttcaaaag tcgtttgggt cagctgaatg cgtccaaaaa
     9361 ctggatgggc agaaaggagc aggcgatggc tttggcgatt gctgcggatt tgcaagacca
     9421 actcttgttc aaaaggctga ttgattttct ggatgcgacc attcactaat tcgcttcgca
     9481 attcctcaac tatgtggtgt aaaaaaaatc cgtcaaatga catcgttctc tccttgtgat
     9541 tgtattccat agtattatat caaaaaggta gaataaaatc atggaaatgt ggtataataa
     9601 agccaagtaa agagaaacga gaagcacatg tatattgaaa tggtagatga aactggtcaa
     9661 gtttcaaaag aaatgttgca acaaacccaa gaaattttgg aatttgcagc ccaaaaatta
     9721 ggaaaagaag acaaggagat ggcagtcact tttgtgacca atgagcgtag tcatgaactt
     9781 aatctggagt accgtgacac cgaccgtccg acagatgtca tcagccttga gtataaacca
     9841 gaattggaaa ttgcctttga cgaagaggat ttgcttgaaa atccagaatt ggcagagatg
     9901 atgtttgagt ttgatgccta tattggggaa ttgttcatct ctatcgataa ggctcatgag
     9961 caggccgaag aatatggtca cagctttgag cgtgagatgg gcttcttggc agtacacggc
    10021 tttttacata ttaacggcta tgatcactac actccggaag aagaagcgga gatgttcggt
    10081 ttacaagaag aaattttgac agcctatgga ctcacaagac aataaacgaa aatggaaaaa
    10141 tcgtgacttg atatccagtt tagaatttgc tttgacaggt atttttactg ctatcaagga
    10201 agaacgcaat atgcgaaaac acgcagtgac ggctctagtg gtcatccttg caggttttgt
    10261 ttttcaggtg tcacgaatcg aatggctctt tctcctattg agcattttct tggtagtagc
    10321 ctttgagatt atcaactctg ctattgaaaa tgtggtggat ttggccagtc actatcactt
    10381 ttccatgctg gctaaaaatg ccaaggatat ggcggctggc gcggtattag tggtttctct
    10441 tttcgcagcc ttaacaggcg cattgatttt tctcccacga atctgggatt tattatttta
    10501 aacagtaaga ggaaattatg acttttaaat caggctttgt agccatttta ggacgtccca
    10561 atgttgggaa gtcaaccttt ttaaatcacg ttatggggca aaagattgcc atcatgagtg
    10621 acaaggcgca gacaacgcgc aataaaatca tgggaattta cacgactgat aaggagcaaa
    10681 ttgtctttat cgacacacca gggattcaca agcctaaaac agctctcgga gatttcatgg
    10741 ttgagtctgc ctacagtacc cttcgcgaag tggacaccgt tcttttcatg gtgcctgctg
    10801 atgaagcgcg tggtaagggg gacgatatga ttatcgagcg tctcaaggct accaaggttc
    10861 ctgtgatttt ggtggtgaat aaaatcgata aggtccatcc agaccagctc ttgtctcaga
    10921 ttgatgactt ccgtaatcaa atagacttta aggaaattgt tccaatctca gcccttcagg
    10981 gaaataatat gtctcgtcta gtggatattt tgagtgaaaa tctggatgaa ggtttccaat
    11041 atttcccgtc tgatcaaatc acagatcatc cagaacgttt cttagtttca gaaatggttc
    11101 gcgagaaagt cttgcaccta actcgtgaag agattccgca ttctgtagca gtagttgttg
    11161 actgtatgaa acgagacgaa gagacagaca aggttcacat ccgtgcaacc atcatggtcg
    11221 agcgcgatag ccaaaaaggg attatcatcg gtaaaggtgg cgctatgctt aagaaaatcg
    11281 gtagtatggc ccgtcgtgat atcgaactca tgctaggaga caaggtcttc ctagaaacct
    11341 gggtcaaggt caagaaaaac tggcgcgata aaaagctaga tttggctgac tttggctata
    11401 atgaaaaaga atactaagta gaggtgggct catgcctgct tcttgttttt acagaaggag
    11461 gacttatgcc tgaattacct gaggttgaaa ccgtttgtcg tggcttagaa aaattgatta
    11521 taggaaagaa gatttcgagt atagaaattc gctaccccaa gatgattaag acggatttgg
    11581 aagagtttca aagggaattg actagtcaga ttatcgagtc aatgggacgt cgtggaaaat
    11641 atttgctttt ttatctgaca gacaaggtct tgatttccca tttgcggatg gagggcaagt
    11701 atttttacta tccagaccaa ggacctgaac gcaagcatgc ccatgttttc tttcattttg
    11761 aagatggtgg cacgcttgtt tatgaggatg ttcgcaagtt tggaaccatg gaactcttgg
    11821 tgcctgacct tttagacgcc tactttattt ctaaaaaatt aggtcctgaa ccaagcgaac
    11881 aagactttga tttacaggtc tttcaatctg cccttgccaa gtccaaaaag cctatcaaat
    11941 cccatctcct cgaccagacc ttggtagctg gacttggcaa tatctatgtg gatgaggttc
    12001 tctggcgagc tcaggttcat ccagctagac cttcccagac tttgacagca gaagaagcga
    12061 cagctattca tgaccaaacc attgctgttt tgagccaggc tgttgaaaaa ggcggttcca
    12121 ccattcggac ctataccaat gcctttgggg aagatggaag catgcaggac tttcatcagg
    12181 tctatgacaa ggctggtcaa gcatgttcac gctgtgggac agtgattgag aaattccagc
    12241 tcggtggacg tggaacccac ttttgtccaa actgtcaaag gagggactga tgggaaaaat
    12301 catcggaatc actgggggaa ttgcctctgg taagtcaact gtgacaaatt ttctaagaaa
    12361 gcaaggcttt caagtagtgg atgccgacgc agtcgtccac caactacaga aacctggtgg
    12421 tcgtctgttt gaggctctag tacagcactt tgggcaagaa atcattcttg aaaacggaga
    12481 actcaatcgc cctctcctag ctagtctcat cttttcaaat cctgaagagc aaaaatggtc
    12541 gaagcaaatt caaggggaga ttatccgtga ggaactggct actttgagag aacagttggc
    12601 tcagacagaa gagattttct tcatggatat tcccctactt tttgagcaag actacagcga
    12661 ttggtttgct gagacttggt tggtctatgt ggaccgagat gcccaagtag aacgcttaat
    12721 gaaaagggac cagttgtcca aagatgaagc tgagtctcgt ctggcagccc agtggccttt
    12781 agaaaaaaag aaagatttgg ccagccaggt tcttgataat aatggcaatc agaaccagct
    12841 tcttaatcaa gtgcatatcc ttcttgaggg aggtaggcaa gatgacagag attaactgga
    12901 aggataatct gcgtattgcc tggtttggta attttctgac aggagccagt atttctttgg
    12961 ttgtatcttt tatgtccatc ttcgtggaaa atctaggtgt agggagtcag caagtcgctt
    13021 tttatgcagg cttagcaatt tctgtctctg ctatttccgc ggcgctcttt tctcctattt
    13081 ggggtattct tgctgacaaa tacggccgaa aacccatgat gattcgggca ggtcttgcta
    13141 tgactatcac tatgggaggc ttggcctttg tcccaaatat ctattggtta atctttcttc
    13201 gtttactaaa cggtgtattt gcaggttttg ttcctaatga aacggcactg atagccagtc
    13261 aggttccaaa ggagaaatca ggctctgcct taggtacttt gtctacaggc gtagttgcag
    13321 gtactctaac tggtcccttt attggtggct ttatcgcaga attatttggc attcgtacag
    13381 ttttcttact ggttggtagt tttctatttt tagctgctat tttgactatt tgctttatca
    13441 aggaagattt tcaaccagta gccaaggaaa aggctattcc aacaaaggaa ttatttacct
    13501 cggttaaata tccctatctt ttgctcaatc tctttttaac cagttttgtc atccaatttt
    13561 cagctcaatc gatcggccct attttggctc tttatgtacg cgacttaggg cagacagaga
    13621 atcttctttt tgtctctggt ttgattgtgt ccagtatggg cttttccagt atgatgagtg
    13681 caggagtcat gggcaagcta ggtgacaagg tgggcaatca tcgtctcttg gttgtcgccc
    13741 agttttattc agtcatcatc tatctcctct gtgccaatgc ctctagcccc cttcaactag
    13801 gactctatcg tttcctcttt ggcttgggaa ccggtgcctt gattcccggg gttaatgccc
    13861 tactcagcaa aatgactccc aaagccggca tttcgagggt ctttgccttc aatcaggtat
    13921 tcttttatct gggaggtgtt gttggtccca tggcaggttc tgcagtagca ggtcaatttg
    13981 gctaccatgc tgtcttttat gcgacgagcc tttgtgttgc ctttagttgt ctctttaatc
    14041 tgattcaatt tcgaacatta ttaaaagtaa aggaaatcta gtgcgagtaa aaatcaatct
    14101 caaatgctcc tcttgtggca gtatcaatta cctaaccagt aaaaattcaa aaacccatcc
    14161 agacaagatt gaggttttaa agtattgtcc caaggaaaga aaagtaaccc tacatcttga
    14221 atctaagtag att
//
LOCUS       DAVKDZ010000049        14107 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000049 DAVKDZ010000000
VERSION     DAVKDZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.050"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            953..2212
                     /locus_tag="VWZ30_001456"
     CDS             953..2212
                     /locus_tag="VWZ30_001456"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9335172.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            2228..2779
                     /locus_tag="VWZ30_001457"
     CDS             2228..2779
                     /locus_tag="VWZ30_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9335173.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICNQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            2772..4049
                     /locus_tag="VWZ30_001458"
     CDS             2772..4049
                     /locus_tag="VWZ30_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9335174.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            4065..4925
                     /locus_tag="VWZ30_001459"
     CDS             4065..4925
                     /locus_tag="VWZ30_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEU9335175.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNISKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            5150..5260
                     /locus_tag="VWZ30_001460"
                     /pseudo
     CDS             5150..5260
                     /locus_tag="VWZ30_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase"
     gene            5306..6110
                     /locus_tag="VWZ30_001461"
                     /pseudo
     CDS             5306..6110
                     /locus_tag="VWZ30_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            6302..8593
                     /gene="pcrA"
                     /locus_tag="VWZ30_001462"
     CDS             6302..8593
                     /gene="pcrA"
                     /locus_tag="VWZ30_001462"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEU9335176.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNIWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFFHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGPGTVEKIRDFANMQDMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            8636..9316
                     /gene="radC"
                     /locus_tag="VWZ30_001463"
     CDS             8636..9316
                     /gene="radC"
                     /locus_tag="VWZ30_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU9335177.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQVSV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(9313..10002)
                     /locus_tag="VWZ30_001464"
     CDS             complement(9313..10002)
                     /locus_tag="VWZ30_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9335178.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGGQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDNVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(10020..10661)
                     /locus_tag="VWZ30_001465"
     CDS             complement(10020..10661)
                     /locus_tag="VWZ30_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU9335179.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(10744..10950)
                     /locus_tag="VWZ30_001466"
     CDS             complement(10744..10950)
                     /locus_tag="VWZ30_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9335180.1"
                     /translation="MFELTYKDCYHVERTLKYEALMLTLSGCVTLPDTLYVTSLTFRG
                     QKVYQGLVEDLYRFLSHADFLHQN"
     gene            complement(10958..11305)
                     /locus_tag="VWZ30_001467"
     CDS             complement(10958..11305)
                     /locus_tag="VWZ30_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEU9335181.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(11310..12425)
                     /locus_tag="VWZ30_001468"
     CDS             complement(11310..12425)
                     /locus_tag="VWZ30_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9335182.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQENHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGIVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(12435..13394)
                     /locus_tag="VWZ30_001469"
     CDS             complement(12435..13394)
                     /locus_tag="VWZ30_001469"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9335183.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(13507..14076)
                     /locus_tag="VWZ30_001470"
     CDS             complement(13507..14076)
                     /locus_tag="VWZ30_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU9335184.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDMTDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
BASE COUNT         4181 a         2570 c         3076 g         4280 t
ORIGIN      
        1 aactatattg ttataaattg atttgaattt caaaattaaa ttgtttgatt cttatttcaa
       61 tttgttatag tatatctgat gtcaaagttc tcggcgagtc aaatagcgat tcccaagcct
      121 gactatcgtg aggtagcgga ttaaaatggt ctggggatag accgttttaa gtctgacgct
      181 ggaaataaga attgtcagaa gaagggatag agaaatcgtg gctctacgaa caggaacgtg
      241 ataataaggc gtatatagcg gataagaggg cctcaaactc taaagtccaa aaaggtagtc
      301 gtaacctata tgcgtaaatc acgagagtaa ttgaattcgt actaagattt tctattttca
      361 ctgtaacctt ttaacgtcct tatatcttgt atacacgagg aaagatgtac gacttatccc
      421 gtgaggtcta tcactataaa gagaaaacga cagatagaag tgatcctgag tcacggttat
      481 ctttaaaata gattgtctga taggacggta tgtataaaac gcttctgtga actgagagaa
      541 ggggagaagt tcttgctaaa atttagttga acagccgtat tccgatactt agataagaga
      601 tctagtctta gcccctactc agttttaggg gataaaaaag gggcaatagc gattcgagaa
      661 agattatact cttcgaaaat ctcttcaaat cacgtcaata tcgccttgcc gtatgtgtag
      721 gatactgact acgtcagttc catctacaac ctcaaaacag tgttttgagc aacctgcggt
      781 tagtttccta gtttgatctt tgattttcat tgagtattag taattcagtt actaactcgt
      841 caactctgat ttatccaata aaattgaaaa ggatggaaaa aaggataaat ttatgatata
      901 ctttattttg aagaccttat tagaaatctt gaaagagtat tgaaaactta gaatgagaaa
      961 aattgttatc aatggtggat taccactgca aggtgaaatc actattagtg gtgctaaaaa
     1021 tagtgtcgtt gccttaattc cagctattat cttggctgat gatgtggtga ctttggattg
     1081 cgttccagat atttcggatg tagccagtct tgtcgaaatc atggaattga tgggagctac
     1141 tgttaagcgt tatgacgatg tattggagat tgacccaaga ggtgttcaaa atattccaat
     1201 gccttatggt aaaattaaca gtcttcgtgc atcttactat ttttatggga gcctcttagg
     1261 ccgttttggt gaagcgacag ttggtctacc gggaggatgt gatcttggtc ctcgtccgat
     1321 tgacttacac cttaaggcgt ttgaagctat gggtgccact gctagctacg agggagataa
     1381 catgaagtta tctgctaaag atacaggact tcatggtgca agtatttaca tggatacggt
     1441 tagtgtggga gcaacgatta atacgatgat tgctgcggtt aaagcaaatg gtcgtactat
     1501 tattgaaaat gcagcccgtg aacctgagat tattgatgta gctactctct tgaataatat
     1561 gggtgcccat atccgtgggg caggaactaa tatcatcatt attgatggtg ttgaaagatt
     1621 acatgggaca cgtcatcagg tgattccaga ccgcattgaa gctggaacat atatatcttt
     1681 agctgctgca gttggtaaag gaattcgtat aaataatgtt ctttacgaac acctggaagg
     1741 gtttattgct aagttggaag aaatgggagt gagaatgact gtatctgaag acagcatttt
     1801 tgtcgaggaa cagtctaatt tgaaagcaat caatattaag acagctcctt acccaggctt
     1861 tgcaactgat ttgcaacaac cgcttacccc tcttttacta agagcgaatg gtcgtggtac
     1921 aattgtcgat acgatttacg aaaaacgtgt aaatcatgtt tttgaactag caaagatgga
     1981 tgcggatatt tcgacaacaa atggtcatat tttgtacacg ggtggacgtg atttacgtgg
     2041 ggccagtgtt aaagcgaccg acttaagagc tggggctgca ctagtcattg ctgggcttat
     2101 ggctgaaggt aaaactgaaa ttaccaatat cgagtttatc ttacgtggtt attctgatat
     2161 tatcgaaaaa ttacgtaatt taggagcgga tattagactt gttgaggatt aaaccgtaga
     2221 ggtgtttatg aatatttgga ccaaattagc aatgttttct ttttttgaaa cggatcgctt
     2281 gtatttgcgt cctttctttt ttagtgatag tcaggacttc cgcgagatag cttcaaatcc
     2341 agaaaatctt caatttattt tcccaacgca ggcaagtctg gaagaaagtc aatatgcact
     2401 ggccaattac tttatgaagt cccctttggg agtgtgggca atttgtaacc agaaaaatca
     2461 acaaatgatt ggttctatta aatttgagaa gttagatgaa atcaaaaaag aagctgagct
     2521 tggctatttt ttgagaaaag atgcttggtc gcaaggattt atgacagagg ttgttagaaa
     2581 aatttgtcag ctttcttttg aggaatttgg cttaaaacaa ttatctatca ttacccacct
     2641 tgaaaatgaa gctagccaaa gagttgctct taagtctgga tttagtttgt tccgtcagtt
     2701 taagggaagt gatcgttaca caagaaaaat gcgggattat cttgaatttc ggtatgtaaa
     2761 aggagagttc aatgagtaag catcaggaaa ttctaagcta tttggaggaa ttaccagtag
     2821 gtaaaagggt cagtgttcgt agcatttcga atcatctagg agttagtgat ggaacagcct
     2881 atcgggctat taaagaagct gaaaaccgtg gaattgtgga gacccgtcct agaagtggaa
     2941 caattcgtgt taaatcccag aaagttgcta tagagagatt aacgtttgct gaaattgcag
     3001 aagtgacttc ttctgaggtt ctggctgggc aagaaggttt agagagagaa tttagtaagt
     3061 tttcaattgg tgccatgact gaacaaaata tcttgtctta ccttcatgat ggggggctct
     3121 tgattgtcgg agaccgaacc cgtattcagt tgctagcctt ggaaaatgaa aatgcagttc
     3181 tggttacagg gggatttcag gttcatgatg atgtgcttaa actggccaat caaaaaggga
     3241 ttcctgttct aagaagtaag catgatacct ttaccgtcgc gaccatgatc aataaagcct
     3301 tgtcaaatgt ccaaatcaag actgatattc tgacagttga gaaactttat cgccctagtc
     3361 atgagtatgg ttttctgaga gagacagata cagttaaaga ttatttggac ttggttcgta
     3421 agaatcgtag cagccgtttc cctgttatca atcaacatca ggtcgttgtt ggcgttgtaa
     3481 ccatgagaga cgctggtgat aaatcaccaa gcacgacaat tgataaggtt atgtctcgta
     3541 gtctattttt ggttggatta tcgacaaata ttgccaatgt gagtcaacgg atgatcgcag
     3601 aagactttga aatggtacca gttgttcgaa gcaatcaaac tttgcttggc gttgtgacgc
     3661 ggcgagatgt catggagaag atgagtcgtt cccaagtttc ggctctacca actttttctg
     3721 agcagattgg acaaaagctc tcttatcacc atgatgaagt agtcattaca gtggaaccct
     3781 ttatgctaga aaaaaatgga gttttggcta atggtgtatt ggcagaaatt ctgacccaca
     3841 tgacccaaga tttagttgtt aatagtggtc gcaatctcat tatcgagcag atgctgatct
     3901 actttttgca ggctgttcag atagatgata tattgcgcat tcaggcacgg attattcatc
     3961 atacgagacg gtcagctata attgattacg atatttatca tggtcaccag attgtttcaa
     4021 aagcaaatgt gactgttaaa attaattaga aactaggaga aaagatgata acattaaaat
     4081 cagctcgtga aatcgaagct atggacaagg ctggtgattt tctagcaagt attcatatag
     4141 gcttacgtga tttgattaag ccaggcgtag atatgtggga agttgaagaa tatgtccgcc
     4201 gtcgttgtaa agaagaaaat ttccttccac ttcagattgg ggttgacggt gccatgatgg
     4261 actatcctta tgctacctgt tgctctctta acgatgaagt ggctcacgct ttccctcgtc
     4321 attatatctt gaaagatggt gatttgctca aagttgatat ggttttggga ggtcccattg
     4381 ctaaatctga cctgaatatc tcaaaattaa acttcaacaa tgttgaacaa atgaaaaaat
     4441 atactcagag ctattctggt ggtttagcag actcatgttg ggcttatgct gttggtacac
     4501 cgtccgaaga agtcaaaaat ttgatggatg taaccaaaga agctatgtac aagggtattg
     4561 agcaagctgt tgttggaaat cgtatcggtg atatcggtgc ggctattcaa gaatacgctg
     4621 aaagtcgtgg ttacggtgta gtgcgtgatt tggttggtca tggtgttggc ccaactatgc
     4681 acgaagaacc aatggttcct aactatggta ttgcaggtcg tggactccgt cttcgtgaag
     4741 gaatggtctt aaccattgaa ccaatgatca atacaggcga ttgggaaatt gatacagata
     4801 tgaaaactgg ttgggcgcat aagaccattg acggtggatt gtcatgtcag tatgaacacc
     4861 aatttgtcat tacgaaagat ggacctgtta tcttgactag ccaaggtgaa gaaggaactt
     4921 attaataaaa agtgaaaaga ctactggaag tttattttga taaaaaatcc agtagatctt
     4981 ttcataataa aacgcattgt atcaagtgtt aggggctgat atcatgcgtt tttctgcttt
     5041 taagattttt tccaactctg tttgtaagcg catcataaca aagggtctag gattcagggc
     5101 tctcctccta tatactatta gtaaagtaaa actaagggag gatattttag tgtcgcagtc
     5161 tattgttcct gtagagattc cacaatattg tcgttttgat tctaaaaaga gaaatggaat
     5221 tctgtttaat gttcgtattg ccaatcttaa atttaccttt tttagattag acttcctgcg
     5281 aaacaaaata tggtatagta gttctatgaa ttatgaagca agtaaacaac taactgatgc
     5341 acgatttaaa tgtcttgttg gtgttcagcg cacgactttt gaagagatgt tagctgtatt
     5401 aaaaacagct tatcaactta aacacgcaaa aggtggacga aaacctaaat taagcctaga
     5461 agactttctt atggccactc ttcaatatgt gcgagaatac cgcacttatg aacaaattgc
     5521 ggctgatttt ggtattcacg aaagcaactt aatccgtcgg agctaatggg ttgaagtaac
     5581 tcttgttcaa agtggtgtta cgatttcaag aactcctctc agttctgagg atacggtaat
     5641 gattgatgcg acggaagtac aaatcaatcg ccctaaaaaa agaattagcg aatgattctg
     5701 gtaaaaagaa atgccacgct atgaaggctc aagcgattgt cacaagtcaa gggagaattg
     5761 tttctttgga tatcgctgtg aactattgtc atgatatgaa gttgttcaaa atgagtcgca
     5821 gaaatatcgg ataagctgga aaaatcttgg ctgacagtgg ttatcaaggg cccatgaaga
     5881 tatatcctca agcacaaact ccacgtaaat ccagcaaact caagccgcta acagttgaag
     5941 ataaagctta taaccatgcg ctatccaagg agagaagcaa ggttgagaac atctttgcca
     6001 aagtaaaaac gtttaaaatg ttttcaacaa cttatcgaaa tcatcgtaaa cgcttcggat
     6061 tacaaatgaa tttgattgct ggtattatca atcatgaact aggattctag ttttgcagga
     6121 agtctattga ggtattgagc tagtttatga aaaaattggg tgaaaaagtc gagtgtttta
     6181 gaaacccaca gtgtagtatt ctagtttcaa tccactatat tttgctactc cccgtaaagt
     6241 ttctattttc cctgatttct gatataatag aaatattgac ttcaagagta aggaagagaa
     6301 gatgaacgca ttattaaatg gaatgaatga ccgtcaggct gaggcggtgc aaacgacaga
     6361 aggtcccttg ctaatcatgg caggggctgg ttctggaaag actcgtgttt taacccaccg
     6421 tatcgcttat ttgattgatg aaaagctggt caatccttgg aatatcttgg ccattacctt
     6481 taccaacaag gctgcgcgtg agatgaaaga gcgtgcttat agcctcaatc cagcgactca
     6541 ggactgtctg attgcgacct tccactccat gtgtgtgcgt attttgcgtc gcgatgcgga
     6601 ccatattggc tacaatcgta attttacaat tgtggatcct ggtgaacagc gaacgctcat
     6661 gaaacgtatt ctcaaacagt tgaacttgga tcctaaaaaa tggaatgaac gaactatttt
     6721 ggggaccatt tccaatgcta agaatgattt gattgatgat gttgcttatg ctgcccaagc
     6781 tggcgatatg tatacgcaaa ttgtggccca gtgttataca gcctatcaaa aagaacttcg
     6841 tcagtctgaa tccgttgact ttgatgattt gattatgctg accttgcgtc tctttgatca
     6901 aaatcctgat gttttgacct actaccagca aaaattccaa tacatccacg ttgatgagta
     6961 ccaagatacc aaccacgctc agtaccaatt ggtcaaactc ttggcttccc gttttaaaaa
     7021 tatctgtgtg gttggggatg cagaccagtc tatctacggt tggcgtggtg ctgatatgca
     7081 gaatatcttg gactttgaaa aggattaccc caaagccaag gttgttttgt tggaggaaaa
     7141 ttaccgctca accaaaacca ttctccaagc ggccaacgag gttattaaaa acaataaaaa
     7201 tcgccgtcct aaaaatatct ggactcaaaa cgctgatggg gagcaaatcg tttactatcg
     7261 tgccgatgat gagctggatg aggctgtatt tgtagccaga accatcgatg aacttagtcg
     7321 cagtcaaaac ttctttcata aggattttgc agttctctat cggactaatg cccagtcccg
     7381 tacaattgag gaagccctgc tcaagtctaa cattccttat accatggttg gcggaaccaa
     7441 attctacagc cgtaaggaaa ttcgcgatat tattgcttat ctcaacctta ttgctaattt
     7501 gagtgacaat attagttttg agcgtattat caacgagcct aaacgaggaa ttggtccagg
     7561 tacagttgag aaaatccgtg attttgcgaa tatgcaagac atgtccatgc tagatgcttc
     7621 tgctaatatt atgttgtctg gtatcaaggg taaggcagcc caatctatct gggattttgc
     7681 caatatgatg cttgatttgc gggagcagct agaccactta agcattacag agttggttga
     7741 gtccgtccta gaaaaaacag gttatgtcga tattcttaac gcccaagcga ctctagaaag
     7801 caaggcacgg gttgaaaata tcgaagagtt tctttctgtt acgaagaact ttgatgacac
     7861 cactgatgtg acagaagagg aaactggtct ggacaaactg agtcgtttct taaatgactt
     7921 ggctttgatt gccgacacag attcaggtag tcaggagaca tcagaagtga ccttgatgac
     7981 cctgcatgct gccaaaggtc tcgaatttcc agttgtcttt ttgattggga tggaagaaaa
     8041 tgtctttcca cttagtcgtg cgactgaaga tccagatgaa ttagaagaag agcgccgtct
     8101 agcctatgta ggtatcacgc gtgcagagaa aattctctat ctgaccaatg ccaactcacg
     8161 cttgcttttt ggtcgtacca attataaccg tccgactcgt tttattaacg aaatcagttc
     8221 agacttgctt gagtatcaag gtctggctcg tcctgcaaat acaagcttta aggcatcata
     8281 tagcagtggt agtatttcct ttggtcaagg tatgagtttg gctcaggctc ttcaagaccg
     8341 taaacgcggt gctgccccaa aatcaatcca gtcaagcggt cttccatttg gtcaatttac
     8401 agctggcgca aaaccagcat ctagcgaggc aaattggtcc attggtgata ttgctctcca
     8461 caagaaatgg ggagagggaa ccgttctgga agtttcaggt agcggtgcta ggcaggaatt
     8521 gaaaatcaat ttcccagaag taggtttgaa aaaactttta gccagtgtgg ctccaattga
     8581 gaaaaaaatc taattttcca tccttctcac gaataataaa gtgaggagga tttttatgta
     8641 cagtatttca ttccaagaag attcactatt accaagagaa aggctggcca aggaaggagt
     8701 tgaagcgctt agtaaccaag agttgctagc tattttactc aggacaggaa cacgtcaagt
     8761 tagcgttttt gaaattgccc aaaaagtctt gaacaatctt tcaagcctaa cggatttgaa
     8821 aaaaatgacc ctgcaggaat tgcagagttt gtctggtatt gggcgtgtta aggccataga
     8881 attacaagct atgattgaac tggggcatcg tattcacaaa cacgagactc ttgaaatgga
     8941 aagtattctc agcagtcaaa agttggccaa gaagatgcag caggaattag gggataaaaa
     9001 acaagagcac ctggtggcac tctatctcaa tactcaaaat caaatcatcc atcagcagac
     9061 catttttatc ggttctgtaa ctcgtagtat cgctgaaccg cgagagattc ttcactatgc
     9121 aatcaagcat atggcgactt ctcttatctt ggtccacaat catccttcag gagcggtagc
     9181 gcctagccaa aatgatgatc atgtcactaa acttgttaaa gaagcctgcg aactgatggg
     9241 gattgttctc ttggaccatt tgattgtctc tcattctaat tactttagtt atcgtgaaaa
     9301 gacagattta atctaaagtt cattaacgac atagtcaaag agtttcttat ctttgggacg
     9361 attttcaaaa agaagttctg gatgccattg gacaccgaga aaggcgacat tatccgtact
     9421 catgacagcc tcaatgatac catctttagg atcatgagcc acaactttta aatttggtgc
     9481 taagtccttg atgctctggt ggtggaagga gttgatatga gagatttctc catagatttc
     9541 ttggagaacg gtatctggtt ctgttaccaa gcgttgagtt gtgtactcaa cagaagaatc
     9601 ctgccaatgg tcttcgatat cttggtacaa agttccaccc atggcaacgt taaagagttg
     9661 ggtaccacgg cagacagaga aaatgggctt tttctgttta atagcttcct tgatgagggc
     9721 cagttcgaag atatctcttt gaaggtgata gtcatcgcta tcaatggttt tgggttcgcc
     9781 atagaatttt ggatcgacat tttgcccacc tgtcaagatg agcttgtcaa tcaaactgat
     9841 atagtggcag gccatttctt gaccaccaat cggtaggatg atgggaatcc ctccagcatc
     9901 tttaacgcct tcaacaaagc cttttgctgc gtagctcatc atgatgtcat catctggatg
     9961 agctttttcg tttcctgtaa tcccaataac tggttttttc ataaaatgat tttcgctttc
    10021 taatcctctt ttcgcatgaa gtagaggagg gtttggagtt cacttgtcaa atcgacatac
    10081 tgaacgacca cgtcttttgg taaatgcaga tggactggtg aaaaactgag aattcctttc
    10141 acaccagcat caaccaagag attagcaact tcttgtgact tgacgctggg aacagttagg
    10201 atagcagtct tcacatcagc atccttgatt ttatccttga tctgagaaat cccgtaaatg
    10261 ggaatcccgt caggagtttg ggtaccgact tcaggatggt catctaggtc aaaggccatg
    10321 ataatcttca tcttgttacg ttcgtggaag cggtagtgga gaagggcatg gcccatattt
    10381 ccaataccaa ccagcatgac attggtgatg gagttatcat tgagcaaatc ggcaaaaaat
    10441 gtcattagtt ttttgacatc atagccaaaa ccacgccgac caagttcacc aaaataggaa
    10501 aaatcacgac gtacggtcgc tgaatcaatc ccaatggcct ctgcaatttg cttagagttg
    10561 gcacgttcaa tcttttctgc atgaaatctc ttaaaaattc gatagtagag agagagtctt
    10621 tttgctgtag cttttggaat agcaaactgt ttatctttca caaaatcaca acctttctat
    10681 tcttctattt tatagaaata ttgtgaaaaa atcaacaaaa ataagaaaaa actaagaaaa
    10741 atcttagttt tgatgtaaaa aatctgcatg agatagaaaa cggtagaggt cttcgaccag
    10801 accctgataa actttctgac ccctaaaagt cagagaagtc acataaagtg tatctggtaa
    10861 ggttacacat cctgacaaag tcaacatgag agcctcatac ttgagagtac gctctacatg
    10921 atagcagtcc ttataggtca gttcaaacat tttggctcta tctttccgat tttgtaaaga
    10981 caccacgttc taccaagcta tccatgagga agtagaattt ttcctgatga atgtggtggt
    11041 cttctgattt gaaaatatca actagacgaa ggccaaactt gtcagtgata ttgattttag
    11101 cccctgtaag ttccttgtta atgatgattt tgagttggaa gccttcaccg ctgtttggca
    11161 ctttttccaa aaggcgagtc agttcatagt taccaacctt agtttcaaaa aaggtgttat
    11221 ctttgagggt gaatttttta acagaagggc taagagtgta atcgtaacga caatttttta
    11281 actgaatgat tttttcaaat gccatatggc taacctccga taatttcttt taaggttttt
    11341 gcgagggttt gtaggtcttc aacggtattt tgtggcgaca aactgatgcg aagggattcc
    11401 ttcaagcgtt ctgaatttgc gccatacatg gcttcaagaa catggctgga ttggacaatg
    11461 cctgcagtac aggctgagcc agtagagatt gaaattccag ctaaatctag ccgaaggagt
    11521 aagaggtcat ttttctgacc aggaaatcca atattgagaa cataagggag atgatgtttt
    11581 cctctattca agtaatactg aattccctcc agctctgcca gaaaggcagt ttctagattt
    11641 tgtacatgtt gaaaatgttc ttcttgtttt tctaggtctt cttttagggc tgcaaccatg
    11701 cctacaatgg caggcagatt ttcagttcct gcacgttttt tctgttcctg gtctccgcca
    11761 tgtagatagg aatcaaagtc catgctagat gcgtagagaa aaccgattcc cttaggacca
    11821 tggaatttgt gggcagaagc agtgagaaaa tcaatgccca attcttctga atgaattggg
    11881 attttaccaa tagcctgaac tgcatcaaca tgataggcag cagggtgttg cttgagtatt
    11941 tggccaattt cagcgatggg cagtaggttt cctgtctcat tattggcaaa catggtggaa
    12001 accaaaatcg tatcgtcacg taaagccttt tgaatttgct gggctgtgat ttcttgattt
    12061 tctggctgga taatggttgc ttcaaaccca aagtgttgaa ccaagtaatc aattgtttca
    12121 aggacagcat ggtgctcgat ggcagttgtg atgatatgtt ttccttgttc ttggtgacga
    12181 agacagtagc caatgatggt agtattattg ccttcagtcc caccagaagt gaaaaagata
    12241 tgttgaggtt ttgtccttag taactgggct agttcctgac gggcttctcg caagagtttg
    12301 ccagcttgac gaccatgacc atgaatacta gaaggatttc cgtggttttc ttgcataacc
    12361 ttggtcatag ctgaaatagc aactgctgac ataggagtcg ttgcagcatt gtccaaataa
    12421 atcaaagaat caccttattt ctttttattg taggcaaaga gtgggctgac tggttttctt
    12481 tcgtgaatac ggacgatagc atcaccaatt aactcactag cagtgatata gcatacattt
    12541 ttaggagttt tttcttttgt tgctactgaa tcagtcacaa gaatttcttt aatattagta
    12601 ttgtcaagaa gctcagcagc tccctcgacg aagagaccgt ggctagaaac agcataaatt
    12661 tctgtagctc cttcacgttc aacgatttta gaagcttcag agaaggtacg tcctgtattt
    12721 aaaatatcat caatcaagat agctttctta ccttcaacat caccaataat ataaccttcg
    12781 ttacgagttg catcgtcttg agggtagtcg ataatggcga taggagcatc aagatattca
    12841 gccaggctac gcgcacgttt gacacctgaa tttttagggc taacgacaac aacatctgaa
    12901 ccaagcaatc ctttatcgca gtaatgtttt gcgaataggg gaacagtgaa aagattatcc
    12961 actggaatat caaagaaacc ttgaacctga acggcatgca aatcaagagt caggatacga
    13021 tcaactccag ccttaaccag catattggca actagttttg ctgtaagtgg ctcacgagga
    13081 caagcaatgc ggtcttgacg tgcatagcca aaatatggaa ggacaacgtt gatactgtgg
    13141 gcacttgcac gcacacaagc atcgaccatg attaacaatt ccattaggtg gttgttgaca
    13201 gggaaacttg ttgattggat gatgtaaaca tcataaccac ggacactttc ttcgatattt
    13261 acttggattt ctccgtctga aaattgacgt gatgatagtt ttccaagtgg gacaccaaca
    13321 gcttgggcaa ttttttgtgc aatctcttgg ttagagttga gtgcgaaaag tttcatgttt
    13381 tttctatctg acattataga ccgtcctctg taaactttat aaatcctagt tatatttacc
    13441 ttacatatat gaactgggat ttgtgtattt ttatcttttc tattttacca aaaaatggag
    13501 attatttcag ctatttttca tacttttgac aaatcgaacc aattttgaag gagctttttg
    13561 gtaggaaatc tgatttttct ctaaaaattg tcggaaatcc tgtttgcctt gctcatgatt
    13621 ttccacttca agctccaatt cgtaatctgt catatcaaag tatcggctct gatccagtgc
    13681 catgagacca atagctgttt tcatttcata gcgaagcgtt gttagacaac caagaacctg
    13741 ccagttctta ctttggatac catgtttcgc caattcatcc agtactagcc cttgaggaag
    13801 ttcttcctta ctcagatagt tctcagcatc ttttagttgc aatttttggt tgtattccat
    13861 gtttccaaca ctctgcggga ctttgagtgt caactcagcc cagtcttcaa aggttcgaat
    13921 gcgcatagcg actttctttt ctcgcagttc aaaatcaggc gtgtctatgt agtaatttgt
    13981 ttgaagaaca ggagtgacac ctgtgaactg gtcttttaga cgattgtatt catctttttt
    14041 caatagtgtt ttcaattcaa tttctaaatg tttcattttt cttacctttt tttatcgttg
    14101 aaagcgg
//
LOCUS       DAVKDZ010000050        14081 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000050 DAVKDZ010000000
VERSION     DAVKDZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14081
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.051"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            83..880
                     /locus_tag="VWZ30_001471"
     CDS             83..880
                     /locus_tag="VWZ30_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU9335185.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVGMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1028..2287
                     /locus_tag="VWZ30_001472"
                     /pseudo
     CDS             1028..2287
                     /locus_tag="VWZ30_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            2383..3657
                     /locus_tag="VWZ30_001473"
     CDS             2383..3657
                     /locus_tag="VWZ30_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEU9335186.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3667..4545
                     /gene="truB"
                     /locus_tag="VWZ30_001474"
     CDS             3667..4545
                     /gene="truB"
                     /locus_tag="VWZ30_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU9335187.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAIGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVVAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSQLTRTSAAGLQLEDALTLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLSPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4786..5088
                     /locus_tag="VWZ30_001475"
     CDS             4786..5088
                     /locus_tag="VWZ30_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9335188.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5196..5834
                     /gene="udk"
                     /locus_tag="VWZ30_001476"
     CDS             5196..5834
                     /gene="udk"
                     /locus_tag="VWZ30_001476"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU9335189.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5962..7302
                     /locus_tag="VWZ30_001477"
     CDS             5962..7302
                     /locus_tag="VWZ30_001477"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU9335190.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLTHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7280..7492
                     /locus_tag="VWZ30_001478"
     CDS             7280..7492
                     /locus_tag="VWZ30_001478"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU9335191.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7489..8364
                     /locus_tag="VWZ30_001479"
     CDS             7489..8364
                     /locus_tag="VWZ30_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU9335192.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8357..9172
                     /locus_tag="VWZ30_001480"
     CDS             8357..9172
                     /locus_tag="VWZ30_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU9335193.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMIQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKVHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9165..9596
                     /locus_tag="VWZ30_001481"
     CDS             9165..9596
                     /locus_tag="VWZ30_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9335194.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9603..11270
                     /gene="recN"
                     /locus_tag="VWZ30_001482"
     CDS             9603..11270
                     /gene="recN"
                     /locus_tag="VWZ30_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU9335195.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKERIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFTKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKINKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDNVTEAALTQARELLRNREK"
     gene            11272..12000
                     /locus_tag="VWZ30_001483"
     CDS             11272..12000
                     /locus_tag="VWZ30_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEU9335196.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHEAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDSKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHYFIENDGFVAED"
     gene            12079..13902
                     /gene="lepA"
                     /locus_tag="VWZ30_001484"
     CDS             12079..13902
                     /gene="lepA"
                     /locus_tag="VWZ30_001484"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU9335197.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGDTYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPVINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPEPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNGKTFDVTEVGIFTPKAVDRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAESLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNQTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         4177 a         2579 c         3344 g         3981 t
ORIGIN      
        1 acttttatga aatatagaaa acgcttccta aaacctaaag tttgtgatat aattatcaag
       61 aaaaaagatt taggagttta ttatggtttc ttcagaattt atctcaaaga ttgaatttgc
      121 ttgcaataag aaagaaagtc tttatagtca aagcaaattt aagtatgcga ttcgttcgat
      181 gttcgcaggt gcatttttaa ccttcagtac tgctgcaggt gcagttgggg ctgacttgat
      241 taataaaatt gcaccaggta gtggacgctt cctctttcca ttcgtttttg cttggggctt
      301 ggcctacatt gtttttttga atgccgagtt ggtcacttca aacatgatgt tcttgactgc
      361 tggtagtttc ttaaaaaaaa tctcttggag aaaaacagct gagattttac tatactgtac
      421 cttgttcaac cttatcggag ccttgatagc agggtggggc tttgctcatt cggcagccta
      481 tgcgaatctg acacacgata gtttcatctc aggtgttgtt gggatgaagt taggccgctc
      541 aaatgaattg gtcttgcttg aggcgatttt ggcaaatatt ttcgtaaata ttgcgattct
      601 gtcatttatt ttggtcaaag atggtggtgc caaactttgg cttgtgttgt cagctattta
      661 catgtttgta ttcttaacaa acgagcacat tgcggcgaac tttgcttctt tcgcgattgt
      721 gaaattcagt gttgctgcgg attcaattgc caacttcggt gttggaaata tgcttcgcca
      781 ctggggtgtg actttcatcg gaaactttat cggaggaggc ctcttgatgg gtcttccata
      841 tgccttcctc aataaaaacg aagatactta tgtagattaa gaaaatgagc acgattgagt
      901 cgtgcttttt tcattttcaa aataaggtaa tagctatttc ttatatcaaa atatagaaaa
      961 ctgatatttg taaactataa ctcaaggtgc tacaatatcc ttaataaaat aatatggagg
     1021 tcaccttatg actcgtgatt ttaaatttga aactctacaa ctacatgctg gtcaagttgt
     1081 ggctccagct actaagtctc gtgcagtgcc gatttatcaa acaacatttt ttgtttttga
     1141 tgacacgtag gaaggtgccg atctgtttgc cttgaggaaa tcagggaaca tttatactcg
     1201 tatcaccaat cctacaacag ctgcccttga aggtggtgtt gaagcgctag caacagcatc
     1261 aggtatgact gcagtgactt atacgatttt ggcgcttgcc catgctggtg agcatgtagt
     1321 ggctgcttcg actatttacg gtggaacctt caatcttttg aaagaacccc ttcctcgtta
     1381 tggtatcaca acaacctttg tcgatgttga taatttggag gaagtagaag cagctatcaa
     1441 agacaatacc aagcttgtct tgattgaaac cttgggtaac cccttgatta atattccaga
     1501 cctggaaaaa ctggcagaga ttgctcataa acatcaaatc ccacttgtgt cagacaatac
     1561 ttttgcaaca ccttatttga ttaacgtctt ctctcatggc gttgacattg ccattcactc
     1621 tgcgactaag tttatcggtg ggcatggtac aactattgga ggaataattg tcgatagtgg
     1681 tcgttttgac tggacggctt cagggaaatt ccctcaattt gttgacgaga gtccaagctg
     1741 ccacaatttg agctatactc atgatgtggg tgcagcagcc tttattatag ctgttcgagt
     1801 tcaattgctt cgtgatacag gtgcagcctt gtcaccattc aatgctttcc tcttgctaca
     1861 aagacttgaa accctttcac ttcgtgtgga acgccatgta caaaatgctg agacaattgt
     1921 tgattttctt gtcaaccatc ctaaggtaga aaaggtaaat tatccaaaac ttgcagatag
     1981 tccttattat gccttggctg agaaatactt gccaaaaggt gtcggttcaa tctttacctt
     2041 ccacgtcaaa ggtgacgagg aggaagcacg caaggtcatt gataatttag aaatcttttc
     2101 tgaccttgca aacgcggcag atgctaaatc gcttgttgtc catccagcaa caatcactca
     2161 cggtcaattg tcagaaaaag acctagaagc agcaggtgtc acaccaaatc aaatccactt
     2221 gtctatcggt cttgaaaatg tagaagattt gattgaagac ttacgcttgg ccttggaaaa
     2281 aatttaaagt aaaagaagat aaacagtggg ttttgactca ctgtttttga ttttccctca
     2341 ggcatgatat aatggttaca gaagtctaga aagaggaatg taatgaacga aatcaaatgt
     2401 cccaactgtg gggaagtctt tacagtaaat gaaagccagt atgctgaact tttgtcccaa
     2461 gtgagaacgg cagagtttga taaggaacta cacgatagga tgaggcagga actggccttg
     2521 gctgagcaaa aggctatgaa tgagcaacag actaaactgg ctcagaagga tcaagaaatt
     2581 gcgcaattgc agagtcaaat ccaaaacttt gatacagaaa aagaattggc caagaaagaa
     2641 gttgaacaga caagccatca ggccttattg gctaaggaca aggaagtaca ggccttggaa
     2701 aaccagttgg cgaccttgcg tttggagcat gaaaatcaat tgcaaaagac cctttctgac
     2761 ctagaaaaag aacgcaatca ggtcaaaaat cagctcctac tgcaagaaaa ggaaaatgag
     2821 ttgtctttgg cttctgttaa gcaaaactac gaagcccagc tcaaagcagc cagtgaacaa
     2881 gtcgagtttt ataagaattt taaggctcaa caatctacaa aagcgattgg ggaaagccta
     2941 gaacagtatg cagagagtga gtttaacaag gttcgtagtt tcgcctttcc aaatgcttac
     3001 tttgagaagg ataacaaggt ctcttcgcgt gggtctaaag gggactttat cttccgtgag
     3061 tgtgatgaaa atggagttga aatcatttct atcatgtttg agatgaaaaa cgaagcggac
     3121 ggaacagaga agaagcacaa gaatgcagat ttttacaagg aattggacaa ggaccgtcgg
     3181 gagaagaact gtgagtatgc cgttttggtg accatgcttg aggctgataa tgactacttt
     3241 aacacaggga ttgttgacgt cagtcacgag tatgaaaaaa tgtatgttgt tcgtcctcaa
     3301 ttctttatcc aattgattgg tctcttacgt aatgcggcgc taaattccct aaaatacaag
     3361 caggagttgg ccttggttcg cgagcaaaat attgacatta cgcattttga ggaagacttg
     3421 gatgccttta agctagcttt tgctaagaac tataattcag cttcgactaa ctttggaaaa
     3481 gctattgatg aaatcgacaa ggccatcaaa cgcatggaag aggttaagaa attcctgact
     3541 acatctgaaa accaactccg tctagctaac aacaaattgg aagatgtctc tgttaaaaaa
     3601 ttgacccgga aaaatccaac catgaaagcg aaatttgaag cattgaaggg ggagtagaaa
     3661 gcaaaaatga acggtattat caacttaaaa aaagaagcag gaatgacctc gcatgatgcg
     3721 gtttttaaac tgcgtaagat tttgggcacc aagaaaattg gtcatggtgg gaccttggat
     3781 ccagatgtag tgggtgtttt gcccattgcg attggcaagg cgacccgcat ggttgagttt
     3841 atgcaggacg agggtaaaat ctatgagggg gaaatcactc tgggctattc cacgacgact
     3901 gaggatgcta gtggggaagt ggtcgcagaa acccctgttt tgtctccctt ggatgaaaag
     3961 cttgttgatg aagcgattgc gagcttgact gggcctatta cccagattcc ccctatgtat
     4021 tcggcagtta aggttaatgg tcgcaagctc tatgagtatg cgcgtgctgg tcaggaagtg
     4081 gagcgtccag aacgtcaggt gaccatttat caatttgagc gaacaagtcc gatttcttat
     4141 gatggccaac ttgcccgatt cacttttcgt gtaaaatgca gtaaagggac ttacatccgt
     4201 actttgtcag ttgatttggg tgaaaagctt ggttatgcgg ctcatatgtc tcagttaact
     4261 cgtactagtg ctgctggctt acaattagaa gacgcactga ccttggagga aattgctgaa
     4321 aaagtagagg ctgggcaatt agattttctc catcctttag agattgggac aggtgatctt
     4381 gtcaaagttt tcctaagtcc agaagaagct acagaagttc gctttggtcg ttttattgag
     4441 ctagaccaaa cggacaaaga actggctgcc tttgaagatg ataaattgtt agccattcta
     4501 gaaaaacgag gcaatctcta taagccaagg aaggttttta gctagattgt ttaggaataa
     4561 aaatcgggtg atagataaca attgcttgat aaaaccccat actaatagta gaatagtttt
     4621 gggaattata atattccaat tgttgcgagt tgtagatact caaataatct atatagaaat
     4681 ttagaggtgt gaaatgaagc aacttaaaat tctttcagat aaatatttag agtccattac
     4741 aggttctggt gggaacttag gcccaggatt tggtgtgata attccatgat gcgaaatgag
     4801 tttcgagaaa gggtggagca acttcttcaa caaaaagaaa taaatgaaaa tagtgagttg
     4861 agtcacctgt ttcgtcttgc tatacaaaat ttagacagaa atgaaaaata ccaatcggtc
     4921 atggccaatt tgagtcaagg gttgtcactt tacctcatga cgcatcatta ccaggcacct
     4981 aagtctgtca ttgattttgg tttatggatt gccaaagctc ctagccagga aagagggaga
     5041 ctggctttct tgcagatgct tgctcaaacc ctacagggct ttcgttaaaa ctgtcgaaca
     5101 tcagagggaa gactctggtg ttttttgctg atgagactat aaattttgta aaaaatgtga
     5161 tagaatagac gacggataaa ataacggagg atagcatgca aaatagacca atcattattg
     5221 gagtgacagg tggttctggt ggtggtaaaa ccagtgtttc aagagccatt ttatcgcatt
     5281 ttcctgatga aaagatttcc atgattgagc atgattcata ctacaaggat cagtctcatt
     5341 tgacctttga agagcgtgtc aaaaccaact atgaccatcc ttttgccttt gatacagact
     5401 tgatgatcga gcagattaag gaattgttgg cagggcgtcc ggtggacatc ccgacctacg
     5461 actatacaga gcatacacgg agtagcaaga cctatcgtca ggagcctcaa gatgtcttta
     5521 tcgttgaggg cattttggtc ttggaggaca agcgtctgcg cgatttgatg gatatcaaga
     5581 tttttgtgga tacggatgac gatgtgcgca ttattcgtcg tatcaagcgt gatatggagg
     5641 agcgtggccg tagccttgat agcgttatta accagtactt aggtgtggtc aaaccaatgt
     5701 accaccagtt tatcgagtca actaagcgtt atgctgatat cgtcattcct gaaggggtta
     5761 gcaataccgt ggctatcgac ctgttgacga ccaagattgc aaagattttg gaagaagctc
     5821 gaaacagcaa ataatcacat gtggaggcct tgcctccttt ttctattttt ccctcataat
     5881 ggtacataaa tggagatttt taggcatatt tttggtataa taatacccat gaaagagcaa
     5941 gtaaatgaat agtaggtgga gatggaaaag tatttatcgg taacaacttt gaccaagtat
     6001 ctgaaaatga aattcgataa agacccatac ttggaacggg tctatttaac tggtcaagtt
     6061 tccaactttc gtaaacgacc tactcaccaa tatttctccc taaaggatga ccatgcagtt
     6121 attcaagcga ccatctggtc tgggatttat cagaaattag ggtttgacct ggaagaagga
     6181 atgaagatca atgtgattgg gcgtgtacag gtctatgaac caagtggtag ctactccatc
     6241 atcattgaaa aagctgagcc tgatggggtt ggggcgcttg cgattcagtt tgaacaactt
     6301 aagaaaaaat tgacagaaga aggcctgttt caagaacgct tcaagcaagc tctaccccaa
     6361 ttttctaaga gaattggggt agtaaccagc cgtagtggag ccgttattcg agatattatc
     6421 acgaccgtca gcaggcgatt cccaggtgtt gacatccttc tttatccgac caaggttcaa
     6481 ggtgaagggg ctgcggagga aattgcaaga aatattgcgc gtgctaatca acgggacgat
     6541 ttggatttgc tcattattgg tcgtggaggt ggttctattg aggatctctg ggcctttaac
     6601 gaagaaattg tggtacgagc tatttttgaa tctcgtttgc cagttatctc tagtgtgggg
     6661 catgagacgg atgtgacctt ggcagatttt gtggcagatc gacgcgctgc aacgccaaca
     6721 gcggcggctg aactggcaac acctgtgacc aagttggatg tattaactca tttgcaaaat
     6781 caggaaaaac ggatggcaac ggcagtccga aatgttctat ctaagaaaca agaggctttg
     6841 aaaaaatgca gtcagtctgt tatctttaga caacctgagc gcttgtatga cggttatttg
     6901 caacgcttgg accaactgca actgcgtttg aaacaaagtt tgcgaactcg gatttctgat
     6961 aacaaacaat tagttcaagc aagaactcat caattagtac aattatcacc tgttaccaaa
     7021 atccaacgct atcaagaccg tttaggacag ttggacaagc tcttaggtag ccaaatggcg
     7081 ttagtttatg acgccaaggt tgctgaggcc aagcgacttt cggaagcttt gctcatgttg
     7141 gatactagcc gaatcgtggc gcgtggttat gctattgtca aaaaagaaga gtccgttgta
     7201 gattcggttg agagtttgaa gaaaaaagac caagtaacgc ttttgatgcg agatggtcaa
     7261 gtagaattag aggttaaaga tgtcaaaaca aaagaaattt gaggaaaatc tagcagaact
     7321 ggaaaccatt gtccaaagtt tggaaaatgg tgaaattgct ctggaagatg cgattactgc
     7381 ctttcaaaag ggcatggtct tgtcaaaaga gctccaagct acgctggaca aggctgaaaa
     7441 gaccttggtc aaggtcatgc aagaagacgg aacagaaagt gattttgaat gaaaaagcaa
     7501 gaaaaattag ctcttgtcga gtcggctttg gaagattttt atggagacca gcagtttgcc
     7561 tctagtttac gggagtctgt tctctattct attcatgctg gtggcaagcg tattcggcct
     7621 tttctcttgt tagaagttct ggaagccttg caggttacca tcaaacctgc tcacgcgcag
     7681 gtagctactg ccttggagat gattcataca gggagcttga ttcacgatga ccttcctgct
     7741 atggatgatg acgattatcg aagagggcgg ttaaccaatc acaagaaatt cggtgaagct
     7801 atggccattt tggctggaga tgccttattc ttagacccat atgccttgat tgcgcaggca
     7861 gatttgccaa gtcagatcaa ggtggacttg attgccaact tatcccttgc ttcaggtagt
     7921 ctgggtatgg tggcagggca agttttggat atggagggcg aacaccagca cttgtctctg
     7981 gaagaacttc agactattca tgccaataag actgggaagt tactagccta tcctttccaa
     8041 gcggcagcta ttatagctga attgtcacct gaaatgcagg tgaagctgaa aactgtgggt
     8101 gaattgattg gacttgcttt tcaagtcaga gatgatgtac tggatgtgac agctagtttt
     8161 gaggaaatcg gcaagacacc tcaaaaggat ctgcaggcag aaaaatcaac ctatcctgcc
     8221 ttgttgggct tggaagagtc cattgccttt tgtaaccaaa ccctggatca agctaatgaa
     8281 aaattagaag aaattgccca gcagcttccc tttgaaacag aatcgattgt aagtgtagta
     8341 gaaagtttga gaatcaatgg ctaaggaaag agtggatgta ctagcttata aacaggggtt
     8401 gtttgaaacg agagagcagg ccaagcgagg tgtgatggct ggcctagtcg tagcagtcct
     8461 taatggagaa cggtttgaca agccaggaga gaaaattcca gatgacaccg aattaaaact
     8521 caagggggag aaactcaagt atgtcagccg tggtggtttg aaactggaaa aggccttgca
     8581 ggtctttgat ttgtcggtgg atggcgcgac tacgattgat atcggggcct ctactggagg
     8641 ttttaccgat gtcatgatac agaatagtgc caagttggtc tttgcagtcg atgttggtac
     8701 caatcagttg gcttggaaat tacgccaaga cccacgagtt gtcagcatgg agcagttcaa
     8761 ttttcgctat gctgaaaaga ctgatttcga gcaggagccg agctttgcca gtattgatgt
     8821 gagtttcatt tcccttagtc tgattttgcc agccttgcac cgtgtcttgg ctgatcaagg
     8881 tcaggtggta gcacttgtca aacctcagtt tgaggcagga cgtgagcaga ttgggaaaaa
     8941 tggaattatt cgagatgcta aggttcatca gaatgtcctt gaatctgtaa cagctatggc
     9001 agtagaggca ggtttttcag tccttggctt ggacttttct cccatccaag gtggacatgg
     9061 aaatattgaa tttttagcgt atttgaaaaa agaaaagtca gcaagcaatc agattcttgc
     9121 tgagattaaa gaagcagtag agagggcgca tagtcaattt aaaaatgaat aaaaaagaaa
     9181 gacttgaaaa aattagaaga ttggtgacag attatcaaat cggcacgcaa gaagaaattg
     9241 tagaacattt gaaagaagca ggtatcactg ccactcaggc gacggtatcc cgagatatca
     9301 aagagttagg tattgtcaaa attcctttga gagacaacac ctatgtctac gaattaccaa
     9361 aatcaatcgt aaaaagtctg caactggctg aagacaatat cgaatcggct gaattgatgg
     9421 ataagatgat caatctccaa gttattccag gaaatacggc ttttgtaaaa gctcagttaa
     9481 tcgagacttt tgcagacaag atttttagct gtttgactga tgatagctcg attttagtca
     9541 ttgccagaag tggaagtcta gcagaggaaa tctttgaaca agtaaaaaat tggtaggtct
     9601 atatgttact tgaaatttcg ataaaaaact ttgccattat tgaagctatt tccctcaatt
     9661 ttgaaaaggg gatgactgtc ctgactggtg aaacgggtgc agggaagtcg attatcattg
     9721 atgccatgaa tatgatgttg ggagctcgtg cgacgacaga tgttattcgt catggtgcac
     9781 caaaggcaga gattgagggg cttttctcag ttgagaatag tcgtctttta caggaaattt
     9841 ttgatgagca aggtttagaa ttaggtgatg aaattatcat ccgtcgggaa atcttgcaaa
     9901 atggtcgtag tatcagccgt gtaaatggtc agatggttaa tctgtctgtt ttgcgagcta
     9961 ttgggcaaca tcttgtagat attcatggtc agcatgacca tgaggagtta atgcgtcccc
    10021 aactgcatat ccagatgttg gatgaatttg gtgatgccgc tttttgggac ttgaaagaaa
    10081 cctatcaaac gagttttgat gcctatcgga aaatgcgcaa gcaggttctg gaagtcaaga
    10141 aaaaccaaca ggaacacaag gaacgtatcg aaatgttgga atttcaaatg gcagagattg
    10201 aggcagcaaa cttgcaggct ggagaagact tggctctcaa ccaagagcga gataaactcc
    10261 tcaaccacaa aaatattgcg gatacactga ccaatgccta cagtatgttg gacaatgaag
    10321 atttttcaag tctggccaat gttcgttcag ctatgaatga catggaaagt gtcgaagagt
    10381 atgatcctga ataccgtgaa atttcaagct ctctgtctga gacctactat gttttagaag
    10441 acattagcaa acgtttggaa gctatcattg aggaccttga ttttgatggc aatcgcctca
    10501 tgcaggttga gaatcgtttg gacctccttc atactattac tcgtaagtat ggtggaactg
    10561 ttgatgatgt tttgctttat tttaccaaga ttacggaaga atacaatctc ttgacaggca
    10621 ataatctttc gtctgaggac atggaagcag agcttaagaa gttggaagtc aatcttgtca
    10681 atttggcagg tcaacttgct tctgctcgtc ataatttagc tcagcaactc gaagctgaga
    10741 ttaaacaaga actgcaagat ctttatatgg aaaaagccca gtttcaggtt cgttttagta
    10801 agggaaaatt cagtcgtgag ggaaatgaaa tggttgagtt ttatatttca accaaccctg
    10861 gagaagactt taaacccttg gttaaggttg cttctggagg ggaattatct cgtctcatgt
    10921 tagccattaa gtctgccttt tcacgtaaag aaggcaagac tagcattgtc tttgatgagg
    10981 tggatacggg agtttcaggt cgtgttgctc aagctattgc tcagaaaatt aataaaattg
    11041 gtcagcatgg tcaggttttg gctatctccc atttgccaca agtaattgcg attgcagatt
    11101 atcaattctt tattgagaag attagtaatg accattcaac ggtttcgact gttcgtcttt
    11161 tgacggtcga agagcgagtg gaggaagttg ccaagatgtt ggcaggtgat aatgtgacag
    11221 aagcagccct gacgcaagcc agagaattgt tgagaaacag ggagaaataa gatgacagat
    11281 tattatgtaa ttggagatgt tcacggaaaa gctgggatgc tggaagacct tctcaaaacc
    11341 tgggatggtc agccccagtt gctctttcta ggtgatttga ttgacagagg tgaggatagt
    11401 caccgtgttc ttgaaatggt caaggacttg gtggacaatc aaggggctat ctgtttgtcc
    11461 ggaaaccacg agtatatgtt tttgacttgg ctagatgacc cagaagaaag ttatgaccat
    11521 tatcgtcgca atggtggaga tacaaccatt aactctatcc taggtcgtcc cttggatgca
    11581 ccagttgatg gagtagaaga tgccaaacgg gttgccgctg aagcggcaga cttggtcgaa
    11641 ttcattcgtc aaatgccatt tgtagtagag acagacaagt atatctttgt tcacgcaggt
    11701 attgatttga ccttggatga ttggcatgaa acaacagatt ataaaaaagt ctggctcaga
    11761 aaaccgttcc acgaagccga aaatcatact ggaaaaacta ttgtcttcgg tcatacaccg
    11821 gtctatggtt tgttacagca agaacgcggt acatctgagc tttggactac agaagatagc
    11881 aagattggca tggacggagg agctgtctat ggtggggtcc ttcacgggat tgtctttact
    11941 gaccagggaa tgacagaaca ctactttatc gaaaatgacg gctttgttgc tgaagattag
    12001 tactcctagc agggtatggt cttgtcaaaa tgtcaaaaac aatttataat aaatagatac
    12061 cctgaaagga agagaatcat gaacttagaa gaattgaaaa aacgacagga gaagatccgt
    12121 aacttctcta ttatcgccca tattgaccac gggaaatcaa ctctagcaga ccgcattttg
    12181 gaaaaaacag agacggtttc aagtcgtgaa atgcaggccc agcttttgga tagcatggat
    12241 ttagaacggg aacgtgggat taccattaag ttgaatgcca tcgagctgaa ttacacggca
    12301 aaagatggcg atacttatat cttccacttg attgacactc cggggcacgt tgactttacc
    12361 tatgaagttt cacgttcgct cgctgcctgt gagggggcta ttttggtggt cgatgctgcc
    12421 caaggaattg aagctcaaac ccttgctaac gtttatctgg ccttggacaa tgatttggaa
    12481 atcatgcctg tcattaataa aattgacctg cctgctgcag atccagagcg cgtgcgtacg
    12541 gagattgagg atgtgattgg tttggatgcc agtgaagcag ttttagcatc tgccaaggct
    12601 ggcatcggta tcgaagagat cctagagcaa attgtagaaa aagtaccaga accaacgggt
    12661 gatgtgacag cgcctcttaa agccttgatt ttcgactctg tttacgatgc ttaccgtggg
    12721 gttattctcc aagttcgtgt tatggatggg gtggttaaac ctggggataa gattcagctc
    12781 atgagtaatg gcaagacctt tgatgtgaca gaagttggta tttttactcc gaaagcagtt
    12841 gatcgtgatt tccttgcgac gggtgatgtt ggttatattg cggcttctat caagacggtt
    12901 caggacaccc gtgtcggtga taccgttact ttggcaacca atcctgcggc agagtcctta
    12961 catggttaca agcagatgaa tcctatggtc tttgcgggtc tctacccaat cgagtcaaac
    13021 aagtacaatg acctgcgtga agcgcttgaa aaattgcaac tgaatgatgc tagtcttcag
    13081 tttgaaccag aaacatctca ggcccttgga tttggtttcc gttgtggatt ccttggactt
    13141 ctccatatgg atgttatcca agaacgttta gaacgtgagt tcaacatcga cctcatcatg
    13201 acagctccat ccgttatcta taaggttaat caaaccgacg gtgagtcaat ggatgtgtct
    13261 aacccatctg agtttccaga cccaactaag attgcgacca ttgaagagcc ttatgttaaa
    13321 gctcaaataa tggtaccgca agagtttgtt ggagcagtga tggaattggc tcagcgtaag
    13381 cgtggggatt ttgtgactat ggattatatt gatgataacc gtgtcaatgt tatctatcaa
    13441 attccgcttg ctgaaattgt ctttgacttc tttgataaac ttaagtcttc gacacgtggt
    13501 tatgcaagct ttgactacga attgtcagaa tatcgcccat ctaagctggt caaaatggac
    13561 atccttctca atggagacaa ggtggatgcc ctcagcttta tcgttcacaa ggactttgcc
    13621 tacgaacgtg ggaaactcat cgttgataag ctcaagaaaa ttatccctcg ccaacaattt
    13681 gaggttccaa ttcaagcagc tattggacac aagattgtgg ctcgtactga tatcaaggcc
    13741 cttcgtaaga acgtacttgc taaatgttat ggtggtgacg tttctcgtaa gcgtaaactg
    13801 cttgaaaaac aaaaagctgg taagaaacgc atgaaagcta tcggatcagt agaagttcca
    13861 caagaagcct tccttagcgt cttgagcatg gacgaagaat aagttattat aattgttttc
    13921 ttgttcacag gaaatttaca taagaatcaa tagtaaaaca ggcttaaatt ggcctgtttt
    13981 tttagttttt atactcttcg aaaatctatt caaaccacgt cagcgtcgcc ttgccgtaga
    14041 tatgatcact gacttcgtca gtcttatcta caacctcaaa a
//
LOCUS       DAVKDZ010000051        13677 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000051 DAVKDZ010000000
VERSION     DAVKDZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13677
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.052"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(<1..977)
                     /locus_tag="VWZ30_001485"
     CDS             complement(<1..977)
                     /locus_tag="VWZ30_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PavB family fibronectin-binding SSURE repeat
                     adhesin"
                     /protein_id="HEU9335198.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVVSGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLPETQGDTVLTEVKPEVVAGNTNSLSTPTEGTEVSEET
                     SPSRLDTLFEKGEETQENPELTDVLKKAVDTADVDGTQASPEDIKKAVDEDVNDTIDV
                     PAAYLEQAKASGPFLAGVNKPLKYELFAGDGMLTRLLLKASDKAPWSDNGVAQNEKIP
                     PVKNLPDGKYYYQVSLNGNTEGKKDQDLLDTLRTNGTNTYEATLTVYEADGDKPDLTK
                     VVKTRNVNITLNGLVTKSDVKSAVENNVKDSIDVPAAYLEQAKGEGPFTA"
     gene            complement(1093..1503)
                     /locus_tag="VWZ30_001486"
     CDS             complement(1093..1503)
                     /locus_tag="VWZ30_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU9335199.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSGTAEHLAKKRQLWTYIQQENPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            complement(1570..2112)
                     /locus_tag="VWZ30_001487"
     CDS             complement(1570..2112)
                     /locus_tag="VWZ30_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9335200.1"
                     /translation="MRKLLSIAISSYNAAAYLHYCVESLVIGGEQVEILIINDGSQDQ
                     TQEIAECLASKYPNIVRAIYQENKGHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLK
                     ILKTLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKG
                     QYIMMHSLIYRTDLLRASQF"
     gene            complement(2418..3083)
                     /locus_tag="VWZ30_001488"
     CDS             complement(2418..3083)
                     /locus_tag="VWZ30_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU9335201.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQNMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            complement(3097..4476)
                     /locus_tag="VWZ30_001489"
     CDS             complement(3097..4476)
                     /locus_tag="VWZ30_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9335202.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTYFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            4694..5026
                     /locus_tag="VWZ30_001490"
     CDS             4694..5026
                     /locus_tag="VWZ30_001490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335203.1"
                     /translation="MKTKASIRELVTGCSKHCFEIVDRTDEVSSKHCFEIVDRTDEVS
                     SKHCFEIVDRTDEVSSKHRFEVADRTDEVSSKHCFEVVDRTDEVSSKHCFEVADRTDE
                     VSNIYTAR"
     gene            complement(5266..6030)
                     /locus_tag="VWZ30_001491"
     CDS             complement(5266..6030)
                     /locus_tag="VWZ30_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU9335204.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLDEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            complement(6247..6804)
                     /locus_tag="VWZ30_001492"
     CDS             complement(6247..6804)
                     /locus_tag="VWZ30_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEU9335205.1"
                     /translation="MTSEYQKMIAGEFYRPSDPELRALAQASRQKQAAFNKEENPLKG
                     AEIIKTWFASTGKNLYINTRLMVDYGVNIHLGENFYSNWNLTMLDICPIRIGDNAMIG
                     PNCQFLTPLYPLDPQERNSGIEYGKPITIGDNFWTGGGVIVFPGVTLGNNVVAGAGAV
                     ITKSFGDNVVLAGNPARVIKEIPVK"
     gene            complement(6910..7764)
                     /locus_tag="VWZ30_001493"
     CDS             complement(6910..7764)
                     /locus_tag="VWZ30_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9335206.1"
                     /translation="MTYEYQSHIYLAEAVLNVKDLVSQTVFYQQIIGLEILSQTDTEV
                     VLGLGGKALVHLIQAQEGGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEALYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEVLAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSRQFYQTVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLDPRKQVLPGLAYYVIEVAHKEELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            complement(8120..12970)
                     /locus_tag="VWZ30_001494"
     CDS             complement(8120..12970)
                     /locus_tag="VWZ30_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9335207.1"
                     /translation="MSRKSIGEKRQSFSMRKLSVGLVSVTLASFFLMSQGIQSVSADN
                     MESPIHYKYMTEGELTDEEKSLLVEALLQLAEESDDTYYLGYIKTKKQDNAGISRTVD
                     GKYSARSDSQPDSTKISDVVHSADSEWSQGQGKVSLQGEASGDDGLSEYTDPVATKGT
                     QEPEHEGEAAVEEELPALEVTTRNRTEIQNIPYTTEEIQDPTLLKNRRKIERQGQAGT
                     RTIQYEDYIVNGNVVETKEVSRTEVAPVNEVVKVGTLVKVKPTVEITNLTKVENKKSI
                     TVSYNLIDTTSAYVSAKTQVFHGDKLVKEVDIENPAKEQVISGLDYYTPYTVKTHLTY
                     NLGENNEENTETSTQDFQLEYKKIEIKDIDSVELYGKENDRYRRYLSLSEAPTDTAKY
                     FVKVKSDRFKEMYLPVKSITENTDGTYKVTVAVDQLVEEGTDGYKDDYTFTVAKSKVE
                     QPGVYTSFKQLVTAMQSNLSGVYTLASDMTADEVSLGDKQTSYLTGAFTGSLIGSDGT
                     KSYAIYDLKKPLFDTLNGATVRDLDIKTVSADSKENVAALAKAANSANINNVAVEGKI
                     SGAKSVAGLVASATNTVIENSSFTGKLIANHQDSNKNDTGGIVGNITGNSSRVNKVRV
                     DALISTNARNNNQTAGGIVGRLENGALISNSVATGEIRNGQGYSRVGGIVGSTWQNGR
                     VNNVVSNVDVGDGYVITGDQYAAADVKNASTSVDNRKADRFATKLSKDQIDAKVADYG
                     ITVTLDDTGQDLKRNLREVDYTRLNKAESERKVAYSNIEKLMPFYNKDLVVHYGNKVA
                     TTDKLYTTELLDVVPMKDDEVVTDINNKKNSINKVMLHFKDNTVEYLDVTFKENFINS
                     QVIEYNVTGKEYIFTPEAFVSDYTAITNNVLSDLQNVTLNSEATKKVLGAANDAALDN
                     LYLDRKFEEVKANIAEHLRKVLAMDKSINTTGDGVVEYVSEKIKNNKEAFMLGLTYMN
                     RWYDINYGKMNTKDLSTYKFDFNGNNETSTLDTIIALGNSGLDNLRASNTVGLYANKL
                     ASVKGEDSVFDFVEAYRKLFLPNKTNNEWFKENTKAYIVEMKSDIAEVREKQESPTAD
                     RKYSLGVYDRISAPSWGHKSMLLPLLTLPEESVYISSNMSTLAFGSYERYRDSVDGVI
                     LSGDALRTYVRNRVDIAAKRHRDHYDIWYNLLDSASKEKLFRSVIVYDGFNVKDETGR
                     TYWARLTDKNIGSIKEFFGPVGKWYEYNSSAGAYANGSLTHFVLDRLLDAYGTSVYTH
                     EMVHNSDSAIYFEGNGRREGLGAELYALGLLQSVDSVNSHILALNTLYKAEKDDLNRL
                     HTYNPVERFDSDEALQSYMHGSYDVMYTLDAMEAKAILAQNNDVKKKWFRKIENYYVR
                     DTRHNKDTHAGNKVRPLTDEEVANLTSLNSLIDNDIINRRSYDDNREYKRNGYYTISM
                     FSPVYAALSNSKGAPGDIMFRKIAYELLAEKGYHKGFLPYVSNQYGAEAFASGSKTFS
                     SWHGRDVALVTDDLVFKKVFNGEYSSWADFKKAMFKQRIDKQDNLKPITIQYELGNPN
                     STKEVTITTAAQMQQLINEAAAKDITNIDRATSHTPASWVHLLKQKIYNAYLRTTDDF
                     RNSIYK"
     gene            complement(13086..13677)
                     /locus_tag="VWZ30_001495"
                     /pseudo
     CDS             complement(13086..13677)
                     /locus_tag="VWZ30_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
BASE COUNT         3912 a         3059 c         2372 g         4334 t
ORIGIN      
        1 gctgtgaatg gaccttcacc cttggcttgt tctaggtagg ctgctggaac atcaatactg
       61 tctttaacgt tgttctcaac tgcagatttg acatcgcttt ttgttacaag accgttcaaa
      121 gtaatattga cattacgtgt cttgacaacc ttagtcaaat caggtttatc tccgtctgct
      181 tcatagacag tcagggtagc ttcataggta tttgtaccat tcgtacgaag tgtatctaat
      241 agatcttgat ccttcttacc ttcagtgtta ccattcaaag atacttgata gtaatactta
      301 ccatcaggta aatttttcac tggtggaatt ttctcgtttt gagcgacacc gttatctgac
      361 catggtgcct tgtcagatgc cttgagcaag agacgagtca acataccatc gcctgcgaaa
      421 agttcgtatt taagcggttt atttacacca gccaagaacg gaccggaagc tttggcttgt
      481 tctaggtagg ctgctggaac atcgatagtg tcattaacat cctcatcgac agctttttta
      541 atatcttctg gacttgcttg tgtcccatcc acatcagccg tatctacagc tttttttaag
      601 acatctgtta gctctggatt ttcttgagtt tcttcacctt tttcaaaaag tgtatccaga
      661 cgggaagggc ttgtttcctc gctcacttca gttccttctg taggtgtaga aagtgagttc
      721 gtatttccag ccacgacctc aggtttcact tcagtcagaa cggtgtctcc ttgagtttct
      781 ggtaagtcac cctctttcgt tcccgatgtc tcttcaggta agacttgacc tggggtcgga
      841 ttgaccacat cggcacgtac agaacttggc tgaccaacta ggacaaagaa gccactaacc
      901 acaacaactg aggcaacacc gacactgaga cggcgaatag accaacgagt atatctttga
      961 tttggattga atttcatagg attctcctta gagtctttct ttatttgatg actctagtat
     1021 aatctcctga aattaaaaag gactaagaaa aagttaaaat ttttcttaaa tccctcttat
     1081 aaaacactta tattaattaa atccataaac agatttggtg atttgataga cgacattgga
     1141 cagtttgcga tctggcaaga cagaatgttt ggtcaaacgg ctcaacatgg tcttgcgaat
     1201 ggcctgaaag acttctggat tttcctgctg aatataggtc cacaattggc gcttttttgc
     1261 cagatgctcc gctgttccag atcggttgag cagggtactg gaaatcaccg tcgtgatttc
     1321 aatatgattc agcagatatt ctcgcatttt gggatgactc acttgggaca aatcaagctg
     1381 gtctaccaag agtcgattga ccttgagttg ctggtcaatg cacttaatca tcacttgctc
     1441 attgacagac tggtcctcac gcccaatcaa ataacgatag aaatcgacag gcagatagta
     1501 catggtcttg acctgctgaa ggggcgtaaa gacaaagaga ttatcgacat aaaaagcatg
     1561 ttcaggcaat tagaactggc tagcacgcaa caaatctgtc cgataaatca gcgagtgcat
     1621 catgatatac tggcctttag agaaatttcc gacctggtcc cagccaaaaa tctgccgaac
     1681 aggcaagact gaatcgtaac tcatactctt cttacgagac tgaccttcct tttcatagac
     1741 aaaattggtc acaaagacat ccacctcttg acccttgctc tcaagttcct gcaaggtttt
     1801 aagaattttc aagtaggcac gaggatccac ccagtcatca ctgtcaacta ctttaaaata
     1861 gcgcccagaa gcctctgcca agccacgatt gaccgcaccg ccatggcctt tattttcctg
     1921 atagatggct ctaacgatgt taggatactt gctagctaaa cactcagcga tttcctgagt
     1981 ctgatcctga gacccgtcat tgataatcaa aatctcaact tgctcaccac caatcactaa
     2041 cgactccaca cagtaatgaa gataggctgc tgcattatag ctagaaatgg cgatagacaa
     2101 taacttccta atctgctcct ttagggactg attttttctt atactcttcg aaaatctctt
     2161 caaaccgcgt caacgtcgcc ttgccgtata gatgttactg acttcgtcag ttctatctgc
     2221 aacctcaaaa cagtgttttg agcagcccgt agctagtttc ctagtttgat ctttgatttt
     2281 cattgagtat tactctctct tgtcacttct ttctatttta ccataaagtt cagcctttga
     2341 agaactttta ctagaagaca aggggcttct gtctctattt gccatcttgg gcatcaaaaa
     2401 agaggggtca tccctcttta cgaattcaat gctactaggg tatccaaata ctggttgttg
     2461 atgactgcca aaatataggt atctgctttc aagaggtcat ctggtccaaa ttcaacatcc
     2521 aatggggaat tttcctgctc tcggaaaccc aaaatattca gattgtattt gccacggagg
     2581 tctaatttac tcagactttg acctgcccaa gactgaggaa ttttcatctc cacgatagac
     2641 acatttttat ccaactgaaa gacatcaaca ctattatgga aaagaatggt ctgtgctaga
     2701 gactgcccca tttcatactc tggcgagata accgagtcag ctccaatctt ttctagcact
     2761 ttcttagcgg tctgactttt gaccttagca ataacagtcg gtacccccaa actcttacag
     2821 tgcataaccg caagcacact cgactccaga ttttcacctg tcgcgactac aacggtatcg
     2881 caggtatcaa tccctgctga tctcaataat tcttcatctg tgatgtcacc aatcactcca
     2941 cgcgccaaaa ctggctcaaa ctgattgatg cgctctgcgt ggtcatcaat agcgataata
     3001 ttcatattct gcttggctag ggcagctagg acactgctcc caaaaattcc caagcccaaa
     3061 attccaatcg tacgatctga catgctcttt cctttcttaa ccaatactaa tatctgcttt
     3121 catatagtga atcatatctt tcttttctgg atggtaatct gccaagctaa caaacaaggt
     3181 caagggacca attcgtccca taaacataag tggcatgata acactgagag ccaatttccc
     3241 aaggtcagga gtcagatttg ccgttacacc aactgtacta agagctgaaa tggtttcaaa
     3301 tatgaggtgg ataaagggag gattgccttt ggctgttatc cctagcagaa tcaatcctat
     3361 caagaagctc atcaaaaaga taataaagac actaaaggat ttttgaaccg ttcgcggcgc
     3421 aatcgttcgt ctcgcaacat tggcatgagg caagcctaga agctcacttc gtgcaaagac
     3481 caagaggaca aaatatgtcg taatcttgag tcccccagct gttcctccag gtgccccacc
     3541 tagaaacatc tgtaagatat aaatcaaaag agtcacagga tgagcctgag tataatctat
     3601 cgtagaaaag ccagctgttc gcatcgtcac tgtttgaaaa aagctaacta aaaccttatc
     3661 ggcaacaggg agattgccaa tcgttccagc attgttccac tcaagaaaga gagtagttgc
     3721 cgttccaaat aacaacaaac ctatagtcaa taatagtaca agcttcgtat gaaagtgcag
     3781 acgtcctttt ttctttcttc ctacatgacc agccaaatca aaccagacca taaaaccaag
     3841 gccgcctgta ataatcaagc ctgcaatcac cagattgacc agtaaatcgg tctgaaaagc
     3901 aaataaactg gtgctcccta aattatcaaa accggcatta cagaaggctg agatcgctag
     3961 aaaaatggaa ctaaaaagac cacgtcccca gccaagttga ggaataaggc gaaaactaag
     4021 caaaatagct cccaagctct caaccaaaaa ggtcgtgaga aaaatagaat agacaaactt
     4081 tctcaaagat cgagtttctc cataactaaa actatcctga atagttgcac ggctacgaag
     4141 actaagcttt tgcttgctct ggatatagaa aaccccaata aaggtcatga gccctagacc
     4201 accgatctga atcaagagca aacagattat ttggccccat atattatagg tgtgagctac
     4261 tggaagggtt gagagacctg tcacacagac tgcagagaca gcagtgaaaa gatgatcaaa
     4321 ataagtcgct cgtgagcttt caacttggac aaagggcaag ctcaaaagaa gagagcctag
     4381 taaaatgacc aaggcaaaac tcaaaaaaat acgacgggct ggcgataatc gagataagcc
     4441 tagctctatt ttttccttag ataatttgaa taacatgttt ccattgtacc ataaaaagct
     4501 cgaaatgact tagataggat aaggcaaaat ccactaacac caagcctaaa aaatgccacc
     4561 tttttattga tagcttaggc aagaacaata cttgatagat gtgactatag gtcaaaaaag
     4621 caagacagga gcttttaacg ccagctactc tatatgcttt tcaggagaaa atttctcttt
     4681 ttgattataa gcaatgaaaa ccaaggcatc gattagagag ctagtcacag gttgctcaaa
     4741 acactgtttt gagattgtgg atagaactga cgaagtcagc tcaaaacact gttttgagat
     4801 tgtggataga actgacgaag tcagctcaaa acactgtttt gagattgtgg atagaactga
     4861 cgaagtcagc tcaaaacacc gttttgaggt tgcagataga actgacgaag tcagctcaaa
     4921 acactgtttt gaggttgtgg atagaactga cgaagtcagc tcaaaacact gttttgaggt
     4981 tgcagataga actgacgaag tcagtaacat ctatacggca aggtgaagct gacctggttt
     5041 gaaaagattt tcgaagagta tcatttcatc tcactctaaa tcttaccaac ataaaacgca
     5101 tagtatcaaa gttttcatca cctgatatga tacgtttttc tgattttaaa gatttttccc
     5161 aacctcaaca tgcctaggac aagaggaagg tgacctcgtt taaacatatt tttgaaaagt
     5221 atgagctaga cattttatga taaaacaaat cctttgccag tacaactaaa ggtggtgaac
     5281 acttgctaaa ctcagttcta gcgccttatt aaaagcttcc gagccccagt cacgactgtc
     5341 gtactggtcc aagtccgcta gagaatctgc tgtgaacaac aattcacccc agagaacccc
     5401 acgcaattga gctactgccg caagagcaga acactccatc tccacaacag cacagccttc
     5461 ttccttacga taagccacct tttcagccgt ttctcggtaa aaaccgtctg tcgtccaggt
     5521 catgacttct tcataaggaa tccctctgtc ttccaaaact tcctcaatag cagcaatagc
     5581 ctctggctgc atttccatat aacgagaagg tgccacatag tggtaactgg ctccttcatc
     5641 tcgcagagcg cgaacaggga ctagaaaggc attttcctct atatcagcta ggacaccaca
     5701 ggtcccagta gagataatct gctccacacc atagccaatc aaccaatcca taaactgggc
     5761 tgctggagcg gagccaacag gagcctgagc cagacagacc tcctcaccct tgtagttgat
     5821 gacatagact ggataggtct tggtagcaga aacgaattcg ccgacacaat ccgcccctac
     5881 ttcccttgca tagcggtcaa tctcctcatc taaaaatgca taaacacact tctttggcaa
     5941 ctgcaagtcc agcccctcgt gattgggcat gataaccgcc tgaggattgt catcaaactc
     6001 taaaatagga atcgcatgtt tctgaatcat agcccacctc ctctaatttt gtactattgt
     6061 atcaaaaact gacaaagtgt caaggaggaa gctgatgaac tgaggagaaa aacatcccac
     6121 ttacgagatg ctttgaactt gcttaatgaa acagacttgc taagagatag agtaggtaaa
     6181 tgtgaactgg gtaaaaaatg atgaaaccta caaaaaagaa acaaccccag ctgttccttt
     6241 ttacttctat ttaacaggta tttccttaat cacgcgcgca ggattgccag ctaggacaac
     6301 gttgtcgcca aaagatttgg taattactgc ccctgctcct gcaacgacat tatttcccag
     6361 tgtcactcca ggaaagacaa tgacgccacc accagtccag aaattatctc cgattgtgat
     6421 gggctttccg tactcgatac ctgaattgcg ttcctgtgga tctagtggat agaggggtgt
     6481 caaaaactga caattaggac caatcatagc attgtccccg atacgaatgg gacagatatc
     6541 cagcatggtc aagttccaat tagaataaaa attttcccct agatggatgt tgacaccgta
     6601 gtccaccatc aagcgagtgt tgatgtaaag atttttcccg gttgaggcaa accaagtctt
     6661 gatgatttcg gctcccttca aggggttctc ttccttgtta aaggcggcct gtttttggcg
     6721 agaagcctga gctaaggctc tcagttctgg gtctgatgga cggtaaaact ctcctgctat
     6781 cattttctgg tattcgctag tcatattatt cttctcctta ttccctacta tcatatcaaa
     6841 ttttccacgt atctgactag aataaggagc actttctaca attgatgctc taccttcatc
     6901 acataccatc tatctagcta aacgaatacg ggtcacgatg ccatctgagt ctgtgatttc
     6961 caattggctc gatgtcatcc atttgattgg tgcgtcaact tcttgtgctc gttgggcaat
     7021 cgttaacagt tcttctttat gtgcgacttc gatgacatag taggctaaac ctggtaggac
     7081 ttgtttacgc ggatccagac cttttcctcc ccattcgttg actgctaaat gatgatggta
     7141 gtccccagct gcgatccaac tagcactagg cacactgaat ttatcctcga gccctaacac
     7201 cgtttgataa aactgtctgg actttcgact atccttgaca gaaagatgaa tatgccccat
     7261 tctcgtaccc tctgctagga taaaaggctc tactctttcc cccaactcat agatatcctg
     7321 agccgcaagg acttcagtca ccccgataat acgtccatct tctcgaatat cccatgtgga
     7381 aactggctta tctcgataga gttcaatgcc atttccctcc aagtcctcta agtaaagggc
     7441 ctcactgtaa ccgtgatctg caccgccaac aagaggaatc tgtaaatccg tcaggtgctt
     7501 caagacatcc gccaaagcct ttcgtgtcgg caaaagaata gccagatggt aaagaccata
     7561 atgttccctt acttctccac cctcttgtgc ttgaatcaag tgtaccaagg cttttcctcc
     7621 aagtcccaga acgacctctg tatccgtttg agataggatt tctaaaccaa taatctgctg
     7681 ataaaaaact gtttgactga ccaaatcctt cacatttaaa actgcctctg ctaaataaat
     7741 gtggctctgg tattcatatg tcataaatat ttcctctttt attgtaactt taatcattac
     7801 aactattata taacttccct caagaaagtc aagccagaca acttgaagca tcccactcag
     7861 caaaaaacaa tcccagaaaa aatcttctag gattgttttg tatttatttt actagactag
     7921 caaagttatt tctcaaatca acattttctg tgggattgaa atccaccttg tcaccattgg
     7981 tcgttgatga gttgaagatg attcctgcaa tctgcattca atcaaatctc tacatcgatc
     8041 cataagaaaa aggagtcgat ttgtcatctg actcctcatc cttacaagaa acactactca
     8101 acaatgaaac tctacaatct tatttatata tagaatttct aaagtcatct gtagtgcgaa
     8161 gatatgcatt atagattttt tgttttaata aatgcaccca acttgctggg gtatgactcg
     8221 ttgcacgatc tatattagta atatctttcg cagccgcttc attaattaat tgttgcattt
     8281 gtgcagccgt tgttatagtt acttcttttg tactattagg attacctaat tcgtattgaa
     8341 ttgttattgg tttcagatta tcttgtttat ctatacgttg tttaaacatt gcttttttga
     8401 aatcagccca tgatgagtac tcaccattga atactttctt aaatactaaa tcatctgtca
     8461 ctaaagcaac atctcttcca tgccatgatg agaatgtttt gcttccgctg gcaaatgctt
     8521 ctgctccgta ctgattagaa acataaggta ggaatccttt gtgataacct ttttccgcaa
     8581 gtaattcata agctattttt ctaaacataa tatctccagg agcacctttc gaattgctta
     8641 gcgctgcgta tacaggagag aacatactta tagtatagta gccatttcgt ttatattctc
     8701 tattatcatc atagctacgt ctatttatga tgtcgttgtc gattaatgag tttaacgatg
     8761 ttaagttagc tacttcttca tctgttaatg gacggacttt atttcctgca tgtgtatctt
     8821 tattatgtct agtatcacga acgtaataat tttctatttt tctaaaccat tttttcttaa
     8881 catcattatt ttgagctaat atcgcttttg cttccatcgc atcaagtgta tacattacat
     8941 catatgatcc atgcatataa ctttgaagcg cctcatccga atcgaaacgt tccaccggat
     9001 tatatgtatg caatctattc aaatcatctt tttctgcttt atataacgta tttaaagcta
     9061 aaatatgaga atttacacta tctacagatt gcagtaaacc aagtgcgtat aactccgctc
     9121 ccaatccttc acgtctacca tttccttcaa agtagattgc agaatcagaa ttatgaacca
     9181 tttcatgagt ataaaccgac gttccataag catctaataa tctatctaac acaaagtgcg
     9241 ttaaacttcc attcgcatac gctcctgcac tactattata ctcataccat ttcccaacag
     9301 gtccgaagaa ttctttaata gagccgatgt ttttatccgt taaccttgcc caataagttc
     9361 ttcctgtctc atcttttaca ttgaatccat cataaactat cacagaacgg aaaagttttt
     9421 cttttgaagc actgtcaaga agattgtacc aaatatcata atggtctcta tgccttttcg
     9481 ctgctatatc aactctattt cttacataag ttcgtaaagc atctcctgaa agaataactc
     9541 catccacact atcacgatat ctttcatacg aaccgaatgc aagtgtagac atattcgatg
     9601 aaatatacac agattcttca ggtaaagtta gtagtggtaa taacatactc ttatgccccc
     9661 aacttggtgc tgatattcta tcgtaaactc ctaatgaata ttttctatcg gctgttggtg
     9721 attcttgttt ttctcgtact tctgcaatat cagacttcat ttcgactata tatgcctttg
     9781 tattttcttt aaaccactcg ttatttgttt tgtttggtaa gaacagtttt ctatacgctt
     9841 ctacgaagtc aaagactgaa tcttctcctt ttaccgatgc aagtttattc gcatataaac
     9901 ctacagtatt tgaagctctc aggttatcta gtccactatt tcctaatgcg ataatagtat
     9961 ccaacgttga agtctcatta tttccgttaa agtcaaactt gtacgtagat aaatcttttg
    10021 tattcatttt accataatta atatcgtacc aacggttcat ataagtaaga cctagcataa
    10081 atgcttcttt gttatttttg attttctcac ttacgtattc aactacaccg tctcctgtag
    10141 tattgattga tttatccatc gctaatactt ttcttaggtg ttctgctata ttagctttaa
    10201 cttcttcaaa ttttctatct aagtataggt tatctaaggc tgcatcattc gctgctccta
    10261 gtactttttt agtagcttct gagttaagtg ttacattttg caagtcgctt agtacgttat
    10321 tcgttatcgc tgtatagtct gaaacaaatg cttctggtgt gaatatatat tcttttcctg
    10381 taacattgta ttcgattact tgactgttta tgaagttttc tttgaatgtt acatctaggt
    10441 attctactgt attatctttg aaatgtaaca taactttatt tattgaattt ttcttattat
    10501 taatatccgt tactacttca tcatctttca tcggcacaac gtctaacaat tctgtagtgt
    10561 aaagtttatc tgttgtcgct actttgttac catagtgaac tactaggtct ttattgtaga
    10621 atggcatcag tttttctatg ttgctataag ctacttttct ttcagattct gctttattta
    10681 gtcttgtata atcaacttct cttagattac gttttaaatc ttgcccagta tcatcaagag
    10741 ttactgtgat tccataatca gcaactttcg cgtctatttg gtcttttgat aattttgtag
    10801 cgaatctgtc tgcttttcta ttatcaactg atgtacttgc atttttcaca tctgctgctg
    10861 cgtattgatc accggtgata acataaccat ctccaacatc tacgttactc acaacattat
    10921 ttactcgacc gttttgccac gtagatccta ctattcctcc gactctagaa tatccttgac
    10981 catttcgtat ttctccagta gcaaccgaat tagatatcaa tgcaccattt tctaatctac
    11041 ctactatccc tccagctgtt tggttattat tgcgtgcatt agtagagatt aaggcatcta
    11101 ccctaacttt attaactctc gaactatttc ctgttatatt acctactatt cctccagtat
    11161 catttttatt actgtcctgg tgatttgcga taagtttccc tgtaaacgag ctgttttcta
    11221 tcactgtatt tgttgcgctc gctactaatc ccgcaacaga tttcgcacct gagatttttc
    11281 cttctactgc aacattatta atattcgcgc tattcgctgc cttcgccagc gctgcgacat
    11341 tttctttact atcagcagaa acagttttaa tatccaaatc tctaactgta gcaccattta
    11401 atgtatcaaa taatggtttt ttcaaatcat aaatggcata cgattttgtt ccatcagaac
    11461 cgatcaagct ccctgtaaat gcacctgtga gataacttgt ctgcttatcg cctaagctca
    11521 cctcatctgc ggtcatatct gaagccaatg tatagacacc agacagattg ctttgcatgg
    11581 ctgttaccag ctgtttaaag gatgtgtaaa ctcctggttg ctctacttta gatttagcta
    11641 cagtaaatgt gtaatcatct ttgtaaccgt ctgtaccttc ttcgacaagt tgatcaacgg
    11701 ctaccgtcac tttatacgtt ccatccgtat tttctgtaat agattttaca ggtaggtaca
    11761 tttctttgaa gcgatctgat ttcactttta caaagtattt agccgtatca gtcggcgctt
    11821 cacttagact taaatatcta cgataacgat cattttcttt accgtataat tctactgaat
    11881 caatatcttt aatctctatt ttcttatact ctaattggaa atcttgagtt gatgtttcag
    11941 tattttcctc attattttca cccaaattat aagttaggtg tgttttaact gtatacggtg
    12001 tgtagtaatc taaacctgat attacttgct ctttggcagg attttctata tccacctctt
    12061 taactagctt gtctccatgg aaaacttgcg tttttgcaga aacatatgct gaggtagtgt
    12121 ctattaagtt ataacttaca gttatagatt ttttgttctc aacttttgtt aagtttgtaa
    12181 tttctactgt aggtttaact ttcacaagtg ttcctacttt aacgacttcg ttgaccggag
    12241 ctacttcagt tcgtgacact tctttagttt ctacgacatt accatttacg atgtagtctt
    12301 catattgaat tgtacgtgtc cctgcttgcc cttgtcgttc aatcttacga cgatttttca
    12361 gaagtgttgg atcctgaatt tcttctgttg tataaggaat attctggatt tccgttctat
    12421 ttcgtgtagt gacctctaaa gccggaagtt cttcttctac tgccgcttcg ccctcatgtt
    12481 cgggttcttg tgtgcctttt gtcgctacag gatcagtgta ctctgaaagt ccatcatccc
    12541 ctgatgcttc accttgtaaa ctaaccttcc cctgtccttg gctccattct gaatcagctg
    12601 aatgaactac atctgatatt tttgtagagt ctggttgact atcacttcga gcagagtatt
    12661 tcccatcgac tgtccttgaa atccctgcat tatcctgttt cttagtcttg atataaccca
    12721 agtaataagt atcatctgat tcttcagcca gttgtaaaag ggcctctacc agcaaggatt
    12781 tttcctcgtc tgtcaattca ccctcggtca tatacttata atgaattgga ctttccatat
    12841 tatcggctga taccgattga atcccttgac tcatcaaaaa gaaactagct aaagtaactg
    12901 ataccaatcc cactgacaac tttctcatcg agaaactctg gcgtttctca ccaatacttt
    12961 ttcggctcat attaacctcg ctttttctac attctttata ccaatctata ttttgtagaa
    13021 taaaagtata aacacaaaaa aatagtatag tatggaattt tctgagtact tttcaataat
    13081 tatactcatc tgctttcaaa aagcattcta gtccatctcc gattaacgat ggactttatc
    13141 acctccttct ccagtccttg tatgacatct tggagttgat tcatgacatc ttccaaagtt
    13201 cgaaaggctt tattcttaaa tccacgttta cgaatctctt tccacacttg ttcaatgggg
    13261 ttcatctctg gtgtgtatgg aggaataaag gtaaagccaa tattactagg atgttctgga
    13321 tgtggcgtaa tatttcgcca accatggcgc ttcaacagtt gatagaaggc atcacgtgtg
    13381 taggaacaac ctaactcctt tttataagcc tgaaataagg catcaattgt aacaaattct
    13441 cctgcctctg cagccttcaa atggcgagca agaaaaactt tctcttcctc aaccgtcata
    13501 tatgcatggt tacgaccacc acgagtttct tgaaggagag agtcgagtcc gaactcctca
    13561 tattttttta catttcgcca aatcgttgtt tgattacact ctaaaagctc tataatctct
    13621 ttataagatt taccccatca gacgaaatag aacgatttga aggcgtttat gatattt
//
LOCUS       DAVKDZ010000052        13614 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000052 DAVKDZ010000000
VERSION     DAVKDZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13614
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.053"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     regulatory      74..162
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            425..985
                     /locus_tag="VWZ30_001496"
     CDS             425..985
                     /locus_tag="VWZ30_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9335208.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            986..2371
                     /locus_tag="VWZ30_001497"
     CDS             986..2371
                     /locus_tag="VWZ30_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9335209.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            2373..3023
                     /locus_tag="VWZ30_001498"
     CDS             2373..3023
                     /locus_tag="VWZ30_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEU9335210.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            3034..3726
                     /gene="tenA"
                     /locus_tag="VWZ30_001499"
     CDS             3034..3726
                     /gene="tenA"
                     /locus_tag="VWZ30_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU9335211.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            3731..4255
                     /gene="thiW"
                     /locus_tag="VWZ30_001500"
     CDS             3731..4255
                     /gene="thiW"
                     /locus_tag="VWZ30_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEU9335212.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERIE"
     gene            4255..5058
                     /locus_tag="VWZ30_001501"
     CDS             4255..5058
                     /locus_tag="VWZ30_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9335213.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVSASPIRKG
                     LVRDLLDYSPTILKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSILRRDENWLDTIKGEVYE"
     gene            5051..5683
                     /locus_tag="VWZ30_001502"
     CDS             5051..5683
                     /locus_tag="VWZ30_001502"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9335214.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMDTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(5892..6683)
                     /gene="thiD"
                     /locus_tag="VWZ30_001503"
     CDS             complement(5892..6683)
                     /gene="thiD"
                     /locus_tag="VWZ30_001503"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9335215.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDTPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(6767..6865)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7049..7444
                     /locus_tag="VWZ30_001504"
     CDS             7049..7444
                     /locus_tag="VWZ30_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEU9335216.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            7455..7826
                     /locus_tag="VWZ30_001505"
     CDS             7455..7826
                     /locus_tag="VWZ30_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU9335217.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYAPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            7836..10079
                     /locus_tag="VWZ30_001506"
     CDS             7836..10079
                     /locus_tag="VWZ30_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9335218.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEAIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYIVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDETEVLGLATSLEEASQHPLAEAIVKRASEAGLEFHTVENFQALHGKGV
                     SGQINGKQVLLGNAKMLDGMDISSIYQEKLEQLEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLKTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKVHEIHKL
                     QAAGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            10286..12061
                     /gene="spxB"
                     /locus_tag="VWZ30_001507"
     CDS             10286..12061
                     /gene="spxB"
                     /locus_tag="VWZ30_001507"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEU9335219.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            12172..12519
                     /locus_tag="VWZ30_001508"
     CDS             12172..12519
                     /locus_tag="VWZ30_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9335220.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVK
                     GPAGLVLDFYRMK"
     gene            complement(12636..13548)
                     /locus_tag="VWZ30_001509"
                     /pseudo
     CDS             complement(12636..13548)
                     /locus_tag="VWZ30_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         3897 a         2620 c         2992 g         4105 t
ORIGIN      
        1 caaggctgta tttccagttc aatttcttga actagtataa aatgtgtgtt ataataatca
       61 cataattaaa taataaagac atttggggtg ctttaggctg agatgatacc cattgaacct
      121 gatacagtta agactggcga agggaaatgt gaaatgtttt tttcgtatgt cgaaaagaac
      181 tgtctaatct tgtaagctga actctaattc gtatctgtaa ttagagtttt ttcttttacg
      241 aaactggatg aagatgatat agtagattga aataagatgc gaacaaatca attagaaaat
      301 tcaaattaat ttctagaaat attttagtgt tcttgtgtac tgttctaaat tcagtctgct
      361 atacaaagta tttaggtcgg tcttcttgag tgccccactt ttctttaaaa aaaggagttt
      421 ttttatgttg aaaaaatggc agttaaaaga tgttatcttg cttgctttct tgtctatctt
      481 ttttggtggg gttttcgttg gttcaggata tgtgtataat attctcagtc tactcttaac
      541 acctcttggt ttgcaggcct ttgccaatga aatcctcttc ggtctctggt gtatggctgc
      601 gcccattgct gccatctttg ttccgagagt cggaagtgca acgattggag aagtgctagc
      661 tgcgcttgct gaagtccttt atggtagcca atttggtcta ggagctcttt tgtctggctt
      721 ggttcaaggt ttgggaagtg aatttggttt tatcgtaact aagaatcgct atgaaagttg
      781 gctctctcta actgctaata gtattgggat tacgcttgtt agctttgtct atgaatacat
      841 taagttaggt tactacgcct tttcccttcc gtttgtcctt tccttgcttg tggtacgttt
      901 tatttctgtt tatttcttct gtaccatctt ggttcgtgcc attgtcaaac tctatcatca
      961 gtttgcaact ggaggaaaag catagatggg tctggaacta cgagcgattc agtccccaat
     1021 cttctctgag ccgtttgatt ttacttttca tgcgcaagcc tttaccttgt tagttgggag
     1081 cagtggttca ggaaaatcca gcctctttca aatgattgcc caagtcagtt ctcttcccta
     1141 tagtggtcaa gtcctgatag atgggagcga ggtcagtcag ctttctatca tcgcacgtgt
     1201 ccagaaggtt ggcattctct ttcaaaatcc caatcatcaa tttaccatgg agaacttgtt
     1261 tgaggagctg attttcacct tggagaatat cggctatcac cttcaggaaa ttgattctaa
     1321 aatagcagag gttgtccagc aatgtcgttg cgaggcaatc ttacaccgtc ccattcatca
     1381 cctatcaggt ggggaaaagc aaaaagcagc gctggctgtc ctctttgcca tgaatcctag
     1441 ggtctatctc ttggatgagc ccttcgcttc tattgaccgc aagagcagaa tcgagatatt
     1501 ggagattcta aaagagttgg ctcttgatgg gaagacagtt attttgtgcg accatgattt
     1561 atctgactat aaagcctata tcgaccatat ggttgagcta agagacggaa aactaaggga
     1621 agtgtttcaa atcccttcct atgagatgac acaggttgct tcaaaggaag ttgcttctag
     1681 cccggaacta ttccatatga accgtgtgac tggtgagctt ggtaatcgcc ccctcttttc
     1741 aattgctgat ttcacattct atcaagggat ttcctgtatc ctgggtgaca atggtgtcgg
     1801 gaaatcaacc ctctttcgct ctattcttca atttcaaaag tataaggggc gcattgcatg
     1861 gaaggggaca gtcctgaaaa agaaaaagag tttgtaccgt gatctgacgg gtgttgttca
     1921 ggaagctgag aagcagttta tccgagtcag tctgcgagag gagcttcaat tagatggacc
     1981 tgattctgaa agaaatcagc ggatttttca agctttacga tattttgatt tggagcaggc
     2041 agtcgataag agtccctatc aattaagtgg tggtcagcaa aaaattcttc agctcttgac
     2101 catcttgacc agtaaggctt ccgtgatctt gctagatgaa ccttttgcag gtttggatga
     2161 tagagcctgc cattattttt gcaagtggat tgtggaggag aggaatcaag gaagaagttt
     2221 tctgctcatt agtcatcgtt tagacccttt gatttctgtg gttgattatt ggattgagat
     2281 gactagtcag ggtcttcggc atgtgaaaga agtgaccatt accaaaccac ttacatctca
     2341 gagtagcaat acccaagggg aagtgagata gtatggtcaa agtagcaacc cagacaccga
     2401 ttatcagtct cttcttgctg attttatcct tggaaacatc tttcattcct tcgattgctc
     2461 tgaatctttc ggtagtcgca ttttgtattc tctttatgct ctattaccgt cgatttaaaa
     2521 tgttagcttg gatgatcata cttgccattt taccatcttt tgccaactac tgggcagttc
     2581 agttacacgg agatgcttca caggcagtca tgcttggaac gagggccttt gtgacagttt
     2641 gtatcggcct tgtctttgtt tcctctattt cactaaaaga gcttctcttg tacttggctc
     2701 aaaaggggct atcacgctct tggtcctatg ccttgattgt ggtattcaat tcttttcctc
     2761 tcattcagca agaaatcaag tccctcaaag aagcttgcct attacgtggt caagaactac
     2821 atttttggtc gcccttgatt tacagtaagg ttctgatgac agtctttagg tggcgccatc
     2881 tttacctgag agctctatct gctcacggat atgacgaaca tgcacagttg aagaatagct
     2941 atcggacttt ttatattcct aaaaaaacaa aattaatcta cctgcttttc tttttattgc
     3001 ttcaaaccag tctattttta taaaggagtt attatggaat ttacagatat tgcgatggaa
     3061 ttatccaaga aagcttggca ggcttccttt catcatccct ttattttaca attacaagag
     3121 gggaatttag aacctgccat tttccgctat tacctgattc aggatgccta ctatctgaag
     3181 gccttctcag aaatctatca tcttttggct gataagactt caaaccaaga gatgaaaaga
     3241 ctcttgaaac aaaatgctca gggtttagtg gagggtgagt tatttattcg ccaacaattt
     3301 ttcaaggaat tggaaatcag cgaccaggaa atggagcaac atccaatcgc tccaacctgt
     3361 tatcattaca tttctcatat ttataggcaa tttgcagagc cgaacttagc catagctttt
     3421 gcaagcttgc ttccttgtcc ttggttatac catgacatag gcaaatcact taatcttaaa
     3481 ccatcaccaa atcctctcta tcaacaatgg attgaaactt atattacgga tgagttagag
     3541 caacagatca gagaggaggg agcactggtc aatcagctct atcgagaaag tgatgagaca
     3601 gataagcaaa aaatgctaga tgccttccac atcagtgttc atatggaagc caagttttgg
     3661 gaaatggcct accaacacca gacatggaag agcgatttac agtctttaga aaaaggagaa
     3721 gaatagaaac atgagaaagc accaactaca agttcacaaa ttgaccattt tatctatgat
     3781 gattgccctt gatgtagtcc ttacacctat ctttcggatt gagggaatgg caccgatgtc
     3841 cagtgtagtc aatattctag cgggaatcat gatgggacct gtttatgcct tggctatggc
     3901 tacagtgaca gcctttatcc gtatgacgac tcaagggatt ccgcctttag ctctcacagg
     3961 agcgactttt ggagcccttc tagcaggtct cttttataag tacggtcgaa aattttactt
     4021 ttctgccttg ggagaaattg tgggaacagg tattattggt tctattgttt cctatcctgt
     4081 tatggtgctc tttacaggat cggctgctaa gcttagttgg tttatctaca ctcctcgatt
     4141 tttcggagca accttgattg gtacagcgat ttcctttatt gcatttagat ttttaatcaa
     4201 gcaggaattc tttaaaaaag tgcaaggata tttctttgct gaaaggatag aatgatgcag
     4261 gcattgacaa atccttttcc tataggctct agttccctca ttcactgtat gaccaatgag
     4321 atttcttgtg agatgctggc aaatgggatt ttggctctgg gatgcaaacc tgtcatggca
     4381 gatgatcccc gtgaggttct tgattttact aagcaaagtc aggctctctt catcaatttg
     4441 gggcatttgt cagctgagaa ggaaaaagca atccgcatgg cagcttcgta tgcaaaccaa
     4501 tcttctctcc cgatggtagt agatgcggtt ggtgtatcag cgtcgcccat tcgtaaggga
     4561 ctagtcagag accttttaga ctatagccct acgatcctta aaggaaacat gtcagaaatt
     4621 cgaagtcttg ttggtttaaa gcaccacggc gttggggtcg atgcgagtgc taaagatcaa
     4681 gaaacggagg atttgcttca agtcttgaaa gattggtgtc agacctatcc tggtatgtct
     4741 ttcttagtta ctggccccaa ggaccttatt gtttcggaga atcaagttgc tgtactggag
     4801 aatggctgta ctgaattaga ctggataaca gggacaggag acttggttgg agccttaaca
     4861 gctgtttttc tcagccaagg aaagactggt tttgaagctt cttgcttagc agtctcttat
     4921 cttaatatcg ctgctgagaa aatagttgtt caaggaatgg gattggaaga atttcgttac
     4981 caagtactca atcagctttc gatcctaaga agagatgaaa attggctaga taccatcaaa
     5041 ggagaggttt atgaatagag aagcacttag actatatctg gtaaccaatc gctatcaaga
     5101 ttccgtggaa agctttcttg caaaagttga gacggcctgc cgttcagggg ttaccatagt
     5161 ccaattgcga gaaaaaaatc tcacaaccaa tcaatattat caactggcaa aacaagtcaa
     5221 ggaaataaca gatgcttatc aggtaccctt gataatcgat gatcggttgg atgtttgtct
     5281 tgcggttgat gcagcaggtc tgcatatcgg agatgatgaa ttaccagttt cggtagctcg
     5341 caaagtcttg ggtcctgaaa aaatcctcgg tgtcaccgct aaaacggtta aaagagctct
     5401 ggaagcggag aaatcaggtg cagattactt gggtacagga gccattttcc caaccaccac
     5461 caaggaaaat gcgcccatta ctctgatatc gaccttgaaa acaatttgcc aaacggttgc
     5521 cattccagta gttgctattg gcggcttgac atcagagaat attgatcagc ttatggacac
     5581 tggcatagct ggtgtagctg ttgtacgtga tttgatgcaa gcagaagata ttgaggcaaa
     5641 aacgcaagcc tttttaacaa agttgcatga cattctttcc taattaatac tcaatgaaaa
     5701 tcaaagagta aactagaaag ctagtcgcag gttgctcaaa gtactgcctt gaggttttag
     5761 ataagactga cgaagtcagc tcaaaacagt gttttgaggt tgcagatgga agctgacgta
     5821 gtttgaagag attttcgaag agtataaaaa ctctctaatt cccttaaagt gggaactaga
     5881 gagttttttt cttaatcttt aaaagttgta tggttgactg gaccagaacc atgaccgagt
     5941 tgaggggcat cttgaatagc ttttgtgata aaggccttgg ccttatcaac tgcctggtaa
     6001 agactcttgc ccttggctag ttcagcagta atcactgcag caaaggtaca tccagtacca
     6061 tgggtgtgac aggtttgaat tcgtgggctt tcccagacaa attgttcatt cttggtaaag
     6121 aggaaatctt tagcaccgcc tttgagatgt ccgcctttga taaccacaga ctgaggacca
     6181 aattctttta aaatcaggcg accagcacgc tgcatgtctt cggggtcatg gattgaaaaa
     6241 ccaacaatct cttctgcttc aggaagattt ggcgtaataa tagttgctag aggtagtaag
     6301 tttgttttga gatagtctct agcatttgag tcaatcaagg catctccact tgtagcaacc
     6361 ataacaggat caaggacata gggacaatcc agttttttaa gatagggttg gatgatttcc
     6421 atgatttcag tagtagccaa cattccagtt tttacagcct gaggtggagt atcagaaaag
     6481 acactctcca attgggcttt caacatttga ggagaaacgt gctcgattag ctgaacacct
     6541 ctggtatttt gagcgacaag actggttaca acagccattc catagacatc tctcgcttgg
     6601 aatgacttta aatctgccat aatgccagca ccaccactag ggtcagtccc tgcaatggtc
     6661 aaagcaacgg gtaaataagt catctaaaac ctcccaacca actgaagttt gtattcttaa
     6721 gaacaagcta gtctgtttta gaaaggcaat agaagactgc tagtccccat tatcatttcc
     6781 acttccatca gctagcatta cctagattga gtcagaggga tctaacagtc gttaatctca
     6841 gctttacgca cccctagtgg ttgttttctt ttttctttag tatagcatat ttttattgtt
     6901 tatatagtaa tatttgactg caaatcgttt ttcaaatgat taaaattcta tttttcgaag
     6961 aaaatattat ttctataatt gacaaatgta gattttgaga gtatactgtt gagtgtaatt
     7021 gacaaatata gattttggag gtatggtaat gcagatttca gatgcagaat ggcaggtcat
     7081 gaagattatt tggatgcagg gggagcagac cagtacagat ttgattaggg ttttggcaga
     7141 gcggtttgac tggtccaagt ccacggttca gacacttttg gctcgtctag ttgagaaaga
     7201 gtgtttgact cggaaaaaag aaggcaagtc ctttgtttat tcagcccttt taactctaga
     7261 ccaaagtcgg gatttacttg tccaagatat caaagacaag gtttgttccc gtaggattag
     7321 gaatttgttg gctgacttga ttgtagaatg tgaatttact cagactgact tggaagactt
     7381 ggaagctgtg atttcagaga agaaatcaag tgctgtaaca gaagtaagat gtaattgtat
     7441 gtaaaggaga cgccatgtta aatagtattg taaccattat ttgtattgcc cttatcgcgt
     7501 ttatcttgtt ttggtttttc aaaaagcctg aaaaatctgg acaaaaagcc cagcaaaaaa
     7561 acggatacca agagattcga gtggaagtca tggggggcta tgctcctgag ttgattgtcc
     7621 tcaagaaatc agtgccagcc cgcattgtct ttgaccgcaa ggatccttca ccatgtctgg
     7681 atcaaattgt ttttccagat tttggtgtac atgcgaacct gccaatgggg gaagagtatg
     7741 tagtggaaat cacgcctgaa caggctggag agtttggctt tgcttgtggt atgaacatga
     7801 tgcacggcaa gatgattgta gagtaggtgg agactatgac agaaattgtg aaagcaagct
     7861 tagaaaatgg cattcaaaaa atccgtatcc gagctgaaaa aggctatcat ccagcccata
     7921 tccagcttca aaagggaatt ccagctgaga ttacctttca tcgtgctact ccttcaaact
     7981 gttataagga aattctgttt gaagaagaag gtatcttgga agcaatcggc gtagatgagg
     8041 agaaagtcat tcgttttaca cctcaagaat tagggagaca tgaattttct tgtggcatga
     8101 agatgcaaaa gggaagctat atagtcgttg agaagactcg aaaatctcta tctctcctgc
     8161 aacgtttttg gattactagt atctttactg tgcctcttgt gattctcatg attgggatgt
     8221 tggcaggtag cattagtcat caagtcatgc attggggaac ctttttagca acaacgccta
     8281 ttatgttagt tgcgggtaag ccatatatcc agagtgcttg ggccagtttt aaaaagcaca
     8341 atgccaacat ggataccttg gttgcgctgg gaactctagt ggcttatttc tatagcctag
     8401 ttgctctctt tgctggtctc cctgtttact tcgaaagtgc tggatttatc ctctttttcg
     8461 ttcttttggg agcagttttt gaggaaaaaa tgagaaaaaa tacgtcccaa gctgtggaga
     8521 aattactgga cttgcaagct aaaaccgcag aagtcttgag tgatgatagt tatgtccaag
     8581 ttcctttgga acaagtcaag gtaggcgacc tgattcgagt gcgtcccggt gaaaagattg
     8641 ctgttgatgg tgtcgtagta gaaggtgtct ctagtattga tgaatccatg gtgacaggtg
     8701 agagtctgcc tgtggacaag acagttggag atactgtcat tggctcaacc atcaatcata
     8761 gtggaacgct tgtctttaga gcagaaaaag ttggctcaga gactgttttg gctcagattg
     8821 tagattttgt gaagaaagct cagacaagtc gtgcgccgat tcaggacttg acggataaga
     8881 tttcagggat ttttgtccca gtagttgtca ttttaggaat catgaccttt tgggtttggt
     8941 tcgtcttgct cagggatagt gtggtcgtgc ttggagctag ctttgtgtcc tctcttctct
     9001 acggagtggc ggttttgatt atcgcctgtc cttgtgcctt gggacttgca acaccgacag
     9061 cccttatggt ggggacagga cgtagtgcca agatgggggt tctcctcaaa aatggaactg
     9121 tcttacagga aatccagaaa gttcaaactc ttgtctttga taagaccggg actttgacgg
     9181 aagggaaacc tgtggtcaca gatattattg gcgacgagac agaagtgctt ggattggcta
     9241 cctccctaga agaagcttcc caacacccat tggctgaagc cattgtgaaa cgagcgagtg
     9301 aagctggact tgagtttcac actgtggaaa atttccaagc cttgcacgga aaaggtgtct
     9361 cagggcaaat caatggaaaa caagttttgc ttggaaatgc taaaatgctg gatggcatgg
     9421 atatttctag catttatcaa gaaaaactag aacaattaga aaaagaagct aagacagttg
     9481 tgttcttggc tgtggacaat gaaatcaaag gcttgcttgc actacaagat attcccaagg
     9541 aaaatgccaa gctagccatc agtcagttga aaaaacgtgg tcttaaaaca gtcatgctga
     9601 caggagacaa tgcaggtgtg gcgcgtgcta ttgcagatca aatcggaatt gaagaggtca
     9661 ttgcaggcgt cttgccagaa gaaaaagtcc atgaaatcca taaactacaa gcggctggca
     9721 aagtagcctt tgttggggat gggattaatg acgctcctgc cctcagtgta gcagatgtgg
     9781 gaattgccat gggggctgga acagatattg ccatcgagtc agcagatttg gtgttgacaa
     9841 ctaataacct cctaggagtg gtgcgtgcct ttgatatgag taagaaaacc tttcatcgta
     9901 ttctgctcaa tcttttctgg gcctttatct acaacgtcgt cggaattccg atagcagcag
     9961 gagtcttttc aggtgttgga ctggctctca atccagaatt ggcaggtcta gccatggcct
    10021 ttagttctgt atctgttctg accagttctc ttctgttaaa ctttagtaaa atagactaaa
    10081 agtaatgact agatttcttt gttgtaaaac agaaatgaca gcactttcaa aactgtatta
    10141 gaatagagaa tagagagttt tgagcagatt tttagaaaag tcagaataaa tatgatacag
    10201 tggaatagta aaaatttgga gaacgtttcc aattctatgt aatcgtattc tccaagtcta
    10261 aaaaaattga aggagagtta tcactatgac tcaagggaaa attactgcat ctgcagcaat
    10321 gcttaacgta ttgaaaacat ggggcgtaga tacaatctac ggtatcccat caggaacact
    10381 cagctcattg atggacgctt tggctgaaga caaagatatc cgcttcttac aagttcgcca
    10441 cgaagagaca ggtgctcttg cagcggttat gcaagctaaa ttcggcggct caatcggggt
    10501 tgcagttggt tcaggtggtc caggtgcgac tcacttgatt aacggtgttt acgatgcagc
    10561 tatggataac actccattcc tagcgatcct tggatcacgt ccagttaacg aattgaacat
    10621 ggatgctttc caagagctta accaaaaccc aatgtacaac ggtatcgctg tttacaacaa
    10681 acgtgtagct tacgctgagc aattgccaaa agtaattgac gaagcctgcc gtgctgcagt
    10741 ttctaaaaaa ggtccagctg ttgttgaaat tccagtaaac ttcggtttcc aagaaatcga
    10801 cgaaaactca tactacggtt caggttcata cgaacgctca ttcatcgctc ctgctttgaa
    10861 cgaagttgaa atcgacaaag ctgttgaaat cttgaacaat gctgaacgcc cagttatcta
    10921 tgctggattt ggtggtgtta aagctggtga agtgattact gaattgtcac gtaaaatcaa
    10981 agcaccaatc atcacaactg gtaaaaactt tgaagctttc gaatggaact atgaaggttt
    11041 gacaggttct gcttaccgtg ttggttggaa accagccaac gaagtggtct ttgaagcaga
    11101 cacagttctt ttccttggtt caaacttccc atttgctgaa gtttacgaag cattcaagaa
    11161 cactgaaaaa ttcatccaag tcgatatcga cccttacaaa cttggtaaac gtcatgccct
    11221 tgacgcttca atccttggtg atgctggtca agcagctaaa gctatccttg acaaagtaaa
    11281 cccagttgaa tcaactccat ggtggcgtgc aaacgttaag aacaaccaaa actggcgtga
    11341 ttacatgaac aaactcgaag gtaaaactga gggtgaattg caattgtatc aagtttacaa
    11401 tgcaatcaac aaacatgctg atcaagacgc tatctactca atcgacgtag gtaacactac
    11461 tcaaacatct actcgtcacc ttcacatgac acctaagaac atgtggcgta catctccact
    11521 ctttgcgaca atgggtattg cccttcctgg tggtatcgct gctaagaaag acaatccaga
    11581 tcgccaagta tggaacatca tgggtgacgg agcattcaac atgtgctacc cagacgttat
    11641 cacaaacgtt caatacgacc ttccagttat caaccttgtc ttctcaaatg ctgagtacgg
    11701 cttcatcaag aacaaatacg aagatacaaa caaacacttg tttggtgtag acttcacaaa
    11761 cgctgactac gctaaaattg cggaagctca aggagctgtt ggattcacag ttgaccgtat
    11821 cgaagacatc gatgcagttg ttgcagaagc tgttaaattg aacaaagaag gtaaaactgt
    11881 tgtcatcgat gctcgcatca ctcaacaccg tccacttcca gtagaagtac ttgaattgga
    11941 tccaaaactt cactcagaag aagctattaa agccttcaag gaaaaatacg aagcagaaga
    12001 actcgtacca ttccgtctct tcttggaaga agaaggattg caatcacgcg caattaaata
    12061 attcctctcg ccgaaaatca aatatgaaac ttgtagactt tattctttga ttttcataga
    12121 gcggaacttt aaaagatcgg agcaattcgg tctttttatg gaggagagta aatgaattta
    12181 aatcaattag atattatcgt ttcaaatgtt ccccaagtct gtgctgactt ggagcatatc
    12241 ttggataaaa aggcagatta tgctgatgat ggttttgctc agtttacgat tggcagtcac
    12301 tgtcttatgt tgtcacaaaa tcatttggtt cctttggaaa actttcagtc aggaatcatt
    12361 attcatatcg aggttgagga tgtagaccag aactacaaac ggttgaacga gcttggtata
    12421 aaggttttac acggaccaac tgtaaccgat tggggaacag agtccttatt agttaaagga
    12481 cctgctggtc tagtgcttga tttttatcgt atgaaatagg tcgcgtagtc atttgacgtt
    12541 aaccttagct atttttaaaa tagaaattga gaatatgaac tattgaaaga tcggagcaat
    12601 ccgatctttt tatataaaga aattgcatat aatactcatc tgctttcaaa aagcattcta
    12661 gtccatttcc gattaacgat ggactttatc acctccttct ccagtccttg tatgacatct
    12721 tggagttgat tcatgacatc ttccaaagtt tgaaaggctt tattcttaaa tccacgttta
    12781 cgaatctctt tccacacttg ttcaatggag ttcatctctg gtgtgtatgg aggaataaat
    12841 gcaaagccaa tattagtcgg aatctttaag atacttgatt tatgccatat agcattgtcc
    12901 ataacgagta aaaaataatc atcttgataa gcttgtgaaa gctcttctaa aaaggcgttc
    12961 atccactcag tattacatct accagctatt aagaaaaatg attcgcctgt atgggcatca
    13021 acagctccat aacaatagcg aaattctcgt atatagtgac tatggacatg tggacctact
    13081 cctattggag accaacaaga tcccagttta ctgattctac cgaaaccagc ttcatcttgg
    13141 tacatcaagc gaaccttacg aaaacgtcta ctggttttaa agcgctttct cgtcttcttg
    13201 aattgagatt ttatttttag acgcaacaat ggtttgagcg tctgctttct taggatgttc
    13261 tggacgtggc ataatatttt gccaaccatg gcgcttcaac agttgataga aggcatcacg
    13321 tgtgtaggaa cgacctaact cctttttata agcctgaaat aaggcatcaa ttgtaacaaa
    13381 ttctcctgtc tctgcagcct tcaaatggcg agcaagaaag gctttctctt cctcaactgt
    13441 catatatgta tggttacgac caccacgtgt ttcttgaagg agagagtcga gtccgaactc
    13501 ctcatatttt tttacatttc gccaaatcgt tgtttgatta cagtctatag tgtattgaat
    13561 ctataacagt acaccttggc tgctaaaata tttctataaa ttaatttgac tgtc
//
LOCUS       DAVKDZ010000053        13489 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000053 DAVKDZ010000000
VERSION     DAVKDZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13489
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.054"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            44..304
                     /locus_tag="VWZ30_001510"
     CDS             44..304
                     /locus_tag="VWZ30_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEU9335221.1"
                     /translation="MDNNFMSFPDRIGTNSSKWRIFETNPNMVSSSLADMDFEIPEFI
                     RSVFIDYMALDFMGYSYQSEKALEAILNWEKKNMGTILIKKN"
     gene            259..636
                     /locus_tag="VWZ30_001511"
     CDS             259..636
                     /locus_tag="VWZ30_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU9335222.1"
                     /translation="MGKKEHGYYFDKEKLILLDSVMTGVANAIQAFTNLGDAVLINDP
                     VYPSFKQIIENNGRKPVFSELEIKDGVYRFNFEKFEDCIITNKVKVFVLCNPHNPLGR
                     AWEIAELNKIGNICLKYNPDFIT"
     gene            756..1025
                     /locus_tag="VWZ30_001512"
     CDS             756..1025
                     /locus_tag="VWZ30_001512"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335223.1"
                     /translation="MAFIKTTTNKEGRTHVYLVEGYRKDGKVRQRILKKYGWMVLDNL
                     IEDKVLETAISEGFFYVKEYNRGKKIVIRCLLCFTKKISIITVVK"
     gene            983..1966
                     /locus_tag="VWZ30_001513"
                     /pseudo
     CDS             983..1966
                     /locus_tag="VWZ30_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611311.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1956..3068)
                     /locus_tag="VWZ30_001514"
                     /pseudo
     CDS             complement(1956..3068)
                     /locus_tag="VWZ30_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611311.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            3330..3526
                     /locus_tag="VWZ30_001515"
                     /pseudo
     CDS             3330..3526
                     /locus_tag="VWZ30_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397596.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            3797..3937
                     /locus_tag="VWZ30_001516"
     CDS             3797..3937
                     /locus_tag="VWZ30_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335224.1"
                     /translation="MEKTQTGADLPQVVIEGYKVTVRVTDKPLSLTDVLDDLLGRQLE
                     KI"
     gene            4037..5605
                     /locus_tag="VWZ30_001517"
     CDS             4037..5605
                     /locus_tag="VWZ30_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9335225.1"
                     /translation="MTRACTYLRLSRDDGDNVESNSIINQRSLLRDYARRHDFTILEE
                     FVDDGISGLTFNRPDFNRMMVMVNNKTIDTIIVKDLSRFGRDYIETGKYLQRIFPAMG
                     IRFISVNDHYDSLTADTNETHLVMPIKSLINDSYCRDISTKVRSTQKAKRQKGEFIGA
                     FAPYGYRKDHNQRNHLVVDEAVRSTIETIFALKLEGYSSNAIANFLNQAGVETPLQRK
                     RACQERIKGFYGYRHKWDTKMVNRILTNRVYTGTLEQGKQTKLNYKSQKSVAVNPEDW
                     VRVEDTHEAIISTSTFSIANNLLLRDVKHGKNTPDLFAGLLFCGDCQAQLLQRKLRYK
                     DKETVQYICSTYNNGQGCSRHAIKEDVLTDLVSTIVHQHLDWKSYLVKSIPDYLDQEH
                     FLEVDFTSLISERQKYEQLLQSLYLDLDDGLINETEYQEFQRSYRQKLKQIEDTIVQK
                     KELAQTLYDKLQDKETWLEQLSTIQHEQELNRLTLVTLLDRIIVHEQQALTLMFHDVA
                     ELSLLQQFSNMEVV"
     gene            5605..7221
                     /locus_tag="VWZ30_001518"
     CDS             5605..7221
                     /locus_tag="VWZ30_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9335226.1"
                     /translation="MARTKNRSRVETVEKIPLQTYRTGIYIRLSKERTESWRHKSQSL
                     KTQESLARTFAQEKGLTVTKCYIDYEYSGTKFNRPAYQDMMEDIKRGVINCILIRDLS
                     RLGRNYIEIGRLIDKVFPFMGVRFISINDNLDTLNGLEDKKSFEVEIKNLVNDLYSKD
                     ISKKVMSYNIHQASQGYYIGTSAPYGYRIDRQEAGARLVIDNQVKPILEFIFQQAIEG
                     VSISQITTELNHRGIAVPQSYRGTGQLYRSEQDIKRWHAGNLLRLIKMPVYRGVLIQR
                     TRNKSLSEEDYIHFENAQEAYVSKDNYEKILFYLDSRKSVKKTSIVRTPNRYRGLVYA
                     KTKSTQMVRRCRHFSNNKSDYDYYYFTDYIYDSETTERKTVYISENALDKIIVELIQS
                     EMKRLGTVETLLPRLETKKDDCLKTYHKQITCHQWTITQLQAELSDLYASFSLNRSER
                     EVYLSQKNEIIAKIETISQEIALCETSIKQIEEEHSKQVNWVRHLAKSQNQVVLTAEL
                     LNALIERIEVDVDKTVTVTFSCQIGGGEHD"
     gene            7214..8653
                     /locus_tag="VWZ30_001519"
     CDS             7214..8653
                     /locus_tag="VWZ30_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9335227.1"
                     /translation="MTKIALYLRLSVDEADTDESNSITNQRYLLNNYLDQTPEFKNWE
                     RLEFVDDGFSGTSLKRPAFQRMMADVKAGTIRHIIVKDLSRFMRDYLELGNYLENIFP
                     FLGVRFIALNDHYDSLVSEHNGLDIDVPFRNLLNDFYAKDVSEKVKSAMNSMKRSGKN
                     MSWLPPFGYIKDPNDRFKIIIDEEVAPIVRRIFRLCIEGMGTQKIAMLLNSEQTITPA
                     ERKMQVSQARYTKSIIITAEQKRNVWTRSGILQILKNEAYKGTYLFNTRTTVNGKQIR
                     RPESEWERLDNNHEAIVSSEDFDKAQQSLTSRISGTKSQVCNQPEEVLLRGLVICEHC
                     GHRLLRYSHNNNNNYFYCCYCRSQGQNMKSCRIDRIEEAIEKELKSRLPMNSPKLSKT
                     NLIREVERLKTQKVSHFQDYKEGLMTRETYMEKRQNLDNSIMEIEEQLKVVGRENIAF
                     DKKLTREVVDTHIERIVVNGQGSFTVVYK"
     gene            8764..9066
                     /locus_tag="VWZ30_001520"
                     /pseudo
     CDS             8764..9066
                     /locus_tag="VWZ30_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            9201..10154
                     /locus_tag="VWZ30_001521"
     CDS             9201..10154
                     /locus_tag="VWZ30_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU9335228.1"
                     /translation="MMETWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNV
                     DRFGEIFPEVEQQEEIVVTAYYPETVDVAAVEADLQARLAELTDFMDLGEVKMGTTAL
                     AEEDWADNWKKYYAPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNT
                     GMENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDK
                     WDMVRESAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            10156..10899
                     /locus_tag="VWZ30_001522"
     CDS             10156..10899
                     /locus_tag="VWZ30_001522"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU9335229.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIENFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            10911..12707
                     /gene="pepF"
                     /locus_tag="VWZ30_001523"
     CDS             10911..12707
                     /gene="pepF"
                     /locus_tag="VWZ30_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9335230.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALANLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAQYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(12767..13444)
                     /locus_tag="VWZ30_001524"
     CDS             complement(12767..13444)
                     /locus_tag="VWZ30_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9335231.1"
                     /translation="MEPDIFIITKLLSDPSRMHILDILMDGKAHTVNEIASFTKIKQH
                     TVSYHLKLLTEAQVTTLQTYGRFHYYSLKSAAIAEMLEFLSFYSPQRDVKSYKQHIHK
                     KELKVARTCYDHIAGELGISITNYLLQENLLSESEKDFQLTEAGKSYFQRKLHIDTDE
                     LKKKKRKFCPKCLDWSERKNHVGGALGNALLEFFSEKQLVIPAQTPRSLTITEQGKEF
                     LEKEWGI"
BASE COUNT         4186 a         2514 c         2950 g         3839 t
ORIGIN      
        1 tttttagagg aggctttaaa atgtaagaga ggaagagaga ataatggata ataattttat
       61 gagctttcca gataggattg gaactaattc aagtaagtgg aggatttttg aaacaaatcc
      121 taatatggtt tcatcttctt tagcagatat ggattttgaa attcctgagt tcattagaag
      181 tgtttttatt gattatatgg cattagattt tatgggctat agttatcaat ctgaaaaggc
      241 tttagaggct attttaaatt gggaaaaaaa gaacatgggt actattttga taaagaaaaa
      301 ctaattttgc tggatagtgt tatgacagga gtagctaatg caattcaggc attcacaaat
      361 ttgggagatg ctgttttaat aaatgatcca gtataccctt cttttaagca aataattgaa
      421 aataatggca gaaaacctgt ttttagcgag ttagaaatta aggatggggt atatcgattt
      481 aattttgaaa agtttgagga ctgtattata acaaacaaag ttaaagtttt tgttctatgc
      541 aaccctcata atcccttagg tagggcttgg gaaattgctg agttaaataa aattggaaac
      601 atttgtttaa agtataatcc cgactttatc acttaatttt taccaagccc tacttaaccc
      661 cttggtacat ttggatttcc caactattcc ccaacttttg tttgttatat atgattatgt
      721 atgattatgt atgattgtgt atgatataat agagtatggc atttattaaa acaacaacca
      781 acaaagaagg acgtactcat gtctatcttg tggaaggata tcgcaaagat ggaaaagtca
      841 gacagcgtat cttgaaaaaa tatggttgga tggtcttgga taacctgatt gaagacaaag
      901 tcctagaaac agctatttct gagggctttt tttatgtcaa ggagtataat agaggtaaga
      961 agattgtaat aaggtgtttg ctatgtttca caaagaaaat ctcgattata accgtcgtca
     1021 agtaggcttc tatacgcttg aagagatagt tccaaaggac cactttctgc gttagttgga
     1081 ggaaactgtc gacttttctg tcatctatga tctagtggag aatagttatt cctcagacaa
     1141 gggacgtcct agccttgacc ctgttatgct ggtcaaaatt cctctgattc agtgcttcta
     1201 tggcattcgc tccaggcgtc agaccatcaa ggacattgag attaatgtcg cttatcgttg
     1261 gtttctgggc ttgactctgg acgataaagt gcctcatttt accacatacg gtaagaacta
     1321 cagtcgtcgt tttgctgaaa cagaattgat cacgcgtatc ttcgagcatg tcctccatct
     1381 ctgtttgact gctggcttag tggatacaga tgagattttc atcgacggaa cgcaaatcaa
     1441 agcagctgcc aacaaccata aaaatagacg acaagaggtt gaggctcaag ctaaatggat
     1501 gagtgaacaa ctcgacaaag aaatagcaca agatcggata acacatggca aaaagcccct
     1561 aaagcccgcc ataaaaggcg aggctaagac caaaaagatg tccatgacag ccaagctttc
     1621 cctgcccttt tcgcaaagct agcaccgttt caccccaact atatcatcgc tgactctggt
     1681 tataaaaatc ccagtattgc caaatgtcta ctgaaccagc atatcacgcc tgtcttttcc
     1741 tacaccagac ctcgtggcaa gaagggcatg ttacgaccga atgatttcat ttatgacgag
     1801 cattataacg tctacctctg tcctgaaaac caagtcttac actacagcac taccaaccgt
     1861 tctgactatc gagaataaga gtaaccccaa gacttgtgcc acttgtctac tcctaaaccg
     1921 ctgtacccaa agtaagacca agcagaagct ggtcaccaga tatgtctcgt tactactttt
     1981 tgaaaattct tgctttgggt acaaatggat aataaaggac aggaaacaca tactttggga
     2041 ttactcttat actcacggta gccttctcga gtggtagtga gatagcttaa tacttgattc
     2101 ctggacagac ataatggtca taacttgcat cataaacaaa attactaggc cttaagttcc
     2161 cctttactcc ctttggacga gtataaggga aaacaggaat gatattacgt tctaacaaat
     2221 aatgggcaat agcttgggtc ttgtaacctg aatctgcaat aatatagtgg ggggagaatg
     2281 gctctaactt tgaaaatagg gcagggaaag cttgactatc atgaacattt cctgcttcaa
     2341 ccgtataagc taaggaccat ccatgcttat cacaagcaac ttgggcagaa taggcaaata
     2401 cttccttgtg ttcgcccttg tgaaacaaac cacactcggg atctgtcgtt gagattttct
     2461 tttccttagc ttcactttct tttgcgggct ttaaggactt ttttcgtgtt ttttcttatc
     2521 taaatcaatc tcaactgcca attgttcact cataaatcta gcttgttggt ctaccatttc
     2581 cttacgggac ttgtgactat tagctgccac tttgatatgg gtaccatcca caaatatttc
     2641 agaagaatca attaaggcaa catacaaagc ttggttgaga acttgggaaa aatctcagaa
     2701 atgagttctt tatcttgaaa acgacggctg taattctttc cataggcagt aaaatgaggg
     2761 accttatcat ctaaagttag tccaagaaac caacgatagg ccacgtttac ttcaatctct
     2821 ttcatggttt ggcgcatgga acgaatgcca taaaaacatt tgatttaagg aattttgact
     2881 aatatgacgg gatcaagact aggacgacca ttatctggac tataggtatc ttctaccaag
     2941 tcatagataa agtcaaaatc aaccttggaa tctacttgtc gaagaaagtg atcttctggg
     3001 accaattcgt ctattgtata gaaaccatat tacttacgat tataatcagg tttttctttg
     3061 tgaaacataa ggaacacctc acaactcttc ttacctctat tatacgactt tacacaaaga
     3121 aaagcccttg gaagcttgac ttccaatgac tgtatcttcg gtctgaaact tctagagtca
     3181 acaaagactt tggaagtttt caatataatc tgttattgga cggttacgta gtttctgatt
     3241 attctttaaa ataaattatt tatgcaagtt ttgcatagaa ccccatcttt atttttagaa
     3301 ttactaaaat agtaattgga aggggttcta tgtatattcg ggtaaaaaat ttaagagaag
     3361 acagtgacta taatcagagt caaattgcac aatatctaaa ttgttctcaa tctacttatt
     3421 ccagaattga gagtggtatt caggatattc ctactcagtt tttaaaaaat tagcaaagtt
     3481 atatggagta actacggatt ttttactaga gatggataag gaataatacc cttcttttgt
     3541 tccttgacaa ctgaatagac caaggtggga ctttaatcat ttgtggagca agttagttag
     3601 atacgctgtg atatgagcga gaattatcag ctataaaata gagtggtact ctatctgcca
     3661 tgatacaagt gaagataatg atacttctaa ccgtttaggc tgccagcgag aaaggacagc
     3721 gggtgtaagc cccatgtaga acttaaccag ttctacccat tgaggtcaac attttgacgt
     3781 aaaggaaata acatttatgg aaaaaacaca aacaggtgcg gatttaccgc aagtcgtgat
     3841 tgagggctat aaggtgacgg tacgagttac cgataaacca ttaagcctaa cagatgtttt
     3901 agatgattta ctgggacggc agctggaaaa aatttgattt tttagcggac aagtcttgat
     3961 aaatgatgta aaatagaata gtagaacctc agtccttaat cggtaagcac ctaaataact
     4021 gattaaggag acaaacatga cacgagcctg tacttattta cgcttatcac gagatgatgg
     4081 ggataacgta gagagtaatt ccatcatcaa ccaacgctcg ctcttgcgag attatgctag
     4141 gaggcatgac ttcaccattt tagaagaatt tgtggatgat ggtattagtg gactgacctt
     4201 taaccgtcca gactttaacc gtatgatggt aatggtaaat aacaagacca ttgataccat
     4261 tatcgtgaaa gacctgtctc gctttggtcg agactatatt gaaactggaa aatacctcca
     4321 gcgaattttt cctgctatgg ggatccgttt tatctcagtt aatgaccatt atgatagctt
     4381 gacggctgat accaatgaaa ctcatctggt catgcccatt aaaagcctca ttaacgatag
     4441 ctattgtcgg gacatttcaa ctaaagtgcg aagtactcaa aaagccaaac ggcaaaaggg
     4501 agagtttatt ggggcttttg ctccttatgg ctatcgtaaa gaccataatc aacgaaacca
     4561 tttagtggtt gatgaagcag ttcgctccac tatcgaaaca atctttgctc tgaaattgga
     4621 aggatattct tctaatgcca ttgcgaattt tttgaatcaa gctggggtgg aaacacctct
     4681 acaacgtaag agagcctgtc aggaaaggat taaaggcttc tatggttatc gtcataagtg
     4741 ggataccaaa atggtcaatc gtatcctgac caatcgtgtc tatactggca ctttagaaca
     4801 aggaaaacag accaaactta attataagtc tcaaaagtct gtggctgtca atccagaaga
     4861 ttgggtacga gtggaagata cccacgaagc cattatctcc acaagtacct tttctattgc
     4921 gaataacctc ttactcagag atgtcaaaca tggaaagaat actcctgacc tctttgcagg
     4981 actcttgttt tgtggcgact gccaggcaca actcttgcag cggaaacttc gttacaagga
     5041 caaagaaact gttcagtata tttgtagcac ctacaacaac ggtcaaggct gtagtcgtca
     5101 tgctattaag gaagatgtgc taaccgattt agtatcaact atcgttcatc agcatcttga
     5161 ttggaagtct tatctagtaa aatctattcc tgattatctt gaccaggaac actttttaga
     5221 ggttgatttt acttctttga tttcggagcg tcaaaagtat gagcagttgc ttcagtccct
     5281 gtatctggat ttagatgacg gattaatcaa tgaaacagaa taccaagagt ttcagcgtag
     5341 ctatcgccaa aagctaaagc aaattgaaga cactatcgtt caaaagaaag aattggctca
     5401 aaccctctac gataagctcc aggataagga gacttggctt gagcagttga gcacgataca
     5461 acatgagcaa gagcttaatc gtctcactct agtgacttta ctggatcgta ttatcgttca
     5521 tgaacaacaa gcattaacct tgatgtttca cgatgtggca gaattgtccc ttcttcaaca
     5581 gttttctaac atggaggtgg tctaatggca cgtactaaga accgctcccg tgtagaaacg
     5641 gtagaaaaga tacctcttca aacctatcga acaggtattt atattcgatt atccaaagaa
     5701 cgtaccgaaa gttggcgaca taaatcgcag tctttaaaga cccaggaaag tctagctaga
     5761 acctttgccc aagaaaaagg tttaacagtc accaagtgct atattgatta tgaatattct
     5821 ggcacaaaat ttaatcgccc agcctatcaa gatatgatgg aagacatcaa gagaggtgtc
     5881 atcaattgta tcttgattcg agacttatca cgcttagggc gtaactacat tgagattggg
     5941 cgactgattg ataaggtatt tccctttatg ggggttcggt ttatttcaat caatgataac
     6001 ctagatactc tcaatgggct agaagataag aaatcctttg aagtggaaat taagaatttg
     6061 gtcaatgacc tttattctaa ggacatttca aagaaagtta tgtcctataa tattcaccaa
     6121 gctagtcaag gatactatat tggaacatct gctccgtatg gttatcgaat tgatcgccaa
     6181 gaagcaggtg cgaggttagt tattgataac caagtcaagc caattttaga gtttattttt
     6241 caacaggcaa ttgaaggtgt atccatatct cagattacta ctgaattgaa tcatagagga
     6301 attgctgtac ctcagtcata ccgtgggact ggccaattgt atagaagtga acaggatatc
     6361 aaaagatggc atgcaggaaa tttgcttcgt cttatcaaaa tgcctgttta caggggagtt
     6421 cttattcaac gaacacggaa caaatcgtta tctgaagagg attatatcca ttttgaaaat
     6481 gctcaagaag cctatgtcag caaagataac tacgaaaaaa tactttttta tctagatagt
     6541 cgtaagtccg ttaaaaagac tagtatagtg agaactccaa accgctatag aggtctggtt
     6601 tacgccaaaa caaaatcaac tcaaatggtt cgcagatgtc gccatttctc aaacaataag
     6661 tctgattatg attactatta ttttacggac tatatctatg attcagagac aactgagaga
     6721 aaaaccgtct atatcagtga aaatgctctg gacaagatta tagtagagtt gatccaatca
     6781 gaaatgaagc ggttaggaac agttgagaca ttgttgccac ggttagaaac taaaaaagac
     6841 gattgcctaa agacgtatca caagcaaata acttgtcatc aatggaccat cacacagcta
     6901 caagctgagc taagtgacct ttatgcgtct tttagtctta atcgttctga acgagaagtc
     6961 tatctctccc aaaagaacga gataatcgca aaaattgaaa ccatttcaca agaaatagcc
     7021 ttatgtgaaa cctcaataaa gcagattgag gaggagcaca gtaagcaggt gaattgggtt
     7081 agacatctag ccaagagcca aaatcaagtt gttttaacgg cagaactctt aaatgcttta
     7141 atagaacgga ttgaggttga tgtagacaaa acagtgacag taactttcag ctgtcagatt
     7201 gggggtggcg agcatgacta aaatcgctct atatctccgc ttgtcagttg atgaggctga
     7261 tacagatgaa agcaactcaa ttacgaacca acggtacttg ttaaacaatt atctcgacca
     7321 aacacctgag ttcaagaatt gggaacgctt ggaatttgtg gacgatggtt tttcaggaac
     7381 cagtcttaaa cgtccagcct ttcaacgcat gatggccgat gtcaaggctg gaactatccg
     7441 tcatatcatc gttaaagact tgtcacgatt tatgagagac tatctggagt tgggaaacta
     7501 cttagagaat atctttcctt ttctcggagt tcgatttatc gctctcaacg accattatga
     7561 tagcctggtg tctgaacata atggactgga tattgatgtg ccctttagaa acttactcaa
     7621 tgatttctac gccaaagacg tttctgaaaa agtcaagagt gcaatgaaca gtatgaaacg
     7681 ctctgggaag aacatgagtt ggttaccacc gtttggttat atcaaggacc caaatgaccg
     7741 atttaagatt atcattgatg aggaagtcgc tccaattgtg agacgaattt ttagactttg
     7801 cattgaggga atgggaactc aaaaaatagc gatgttactt aatagtgaac agacgattac
     7861 tccagctgag aggaaaatgc aggttagtca agcaaggtat acaaaatcaa ttatcattac
     7921 tgccgaacaa aagaggaatg tatggacgag aagtggtatt ctccaaatac tcaaaaatga
     7981 agcctataag gggacatacc tgtttaacac tagaacaacg gtaaatggta agcagattcg
     8041 gcggcctgaa tcagagtggg aacgtcttga caataatcat gaggcaattg tatcaagtga
     8101 ggactttgac aaagctcaac aatcacttac ctctcgcatt agtgggacga aaagtcaggt
     8161 ttgtaatcag ccagaagaag ttttattgag aggcttagtt atctgtgaac attgtgggca
     8221 tcgtctcctt cgatatagcc acaataacaa caataattat ttttattgtt gttattgtag
     8281 aagccaaggc cagaatatga agtcctgtcg gatagataga atagaagagg ctatcgagaa
     8341 agaattaaag tctcggttgc cgatgaactc tccaaagcta tcaaaaacga atcttataag
     8401 agaagttgaa cggctgaaaa ctcaaaaggt ttctcatttt caagactata aagagggctt
     8461 gatgacaaga gaaacttaca tggaaaaacg tcagaactta gataattcta taatggaaat
     8521 agaagaacaa ttaaaggtag ttggtaggga aaatatagca tttgacaaga aattgaccag
     8581 agaagtagtt gacactcata tcgagaggat tgtcgtcaat ggccaaggtt cttttacagt
     8641 cgtctataaa taacctaatg agagacttgt gtaatagcaa gtctctttct tgaaaagtag
     8701 ctttgtggta taataagggt gttttcagtg tagaaaaata gtgtcattta cttgacataa
     8761 gagggtggac gtgaaggtga tgaaagtcct tttgagtgcg cagcgcgtga agtttatgaa
     8821 gaactgggca ttcatctgac tgaagattgt ctgctttgga gtaaggttta cccaagtatg
     8881 ctttttgcag ataaacagtc cgtttttcta gttggtcagt tgacacagaa ccagtttgac
     8941 agtatcgtat ttggagatga aggacagggc tatcagctga tgaatgttga ggaatttctt
     9001 agttccagtc aagttgtgcc tcagttgcaa gagagattaa aagattattt aaaagtaagt
     9061 gattagaaag gatggttcag ttaaatttct aaactgaacc cgccctaaac actgtgccaa
     9121 aaagataaac ttctcttaga cacaagcgtc ttcagagaat ttcctatttt ggttttgtgt
     9181 tttacgggct tggtatctta atgatggaaa catggcaaga gttaaaagtt acagtgaagc
     9241 gtgaggggga ggaattggtt tccaatctct tgattgagct gggagcgcaa ggagtcgcaa
     9301 tcgaagacag tatggactat gtgggaaatg tggaccgctt cggcgagatt ttcccagagg
     9361 tggagcagca agaagaaatc gtagtgacag cctactaccc tgaaacggtt gatgtagcag
     9421 cagttgaagc ggatttgcag gctcgtctgg cagaattgac cgactttatg gacctaggag
     9481 aagtcaagat gggcacgact gccttggctg aggaagactg ggctgacaac tggaagaagt
     9541 attatgcacc ggcacgcatc acccatgacc tgaccatcgt accgtcatgg acggactatg
     9601 aggcaacagc aggagaaaag attatcaagc tggatcctgg tatggccttt gggacaggga
     9661 cccacccaac aaccaagatg agcctctttg ccttggaaca ggttcttcgt ggtggcgaaa
     9721 cagtgctaga tgtagggact ggttcagggg ttctttctat tgcaagctca ctcctaggtg
     9781 ccaaggagat tttcgcctat gacttggatg atgtggcggt tcgtgtcgct caggaaaata
     9841 ttgagctcaa cactggtatg gaaaatatcc atgtagcagc aggagacttg cttaagggag
     9901 ttgagattga agcagatgtc atcgtggcta atatcttggc ggatattctc attcatctga
     9961 cggaggatgc ttatcgcttg gtaaaggatg aaggctacct catcatgagt ggcattatca
    10021 aggataagtg ggacatggtg cgcgagtcgg ctgaatcagc tggatttttc ctcgaaactc
    10081 acatggttca aggggaatgg aatacctgtg tctttaagaa aaccaaggat atctctggtg
    10141 tgattggagg ctagcatgca gcagtatttt gtaaaaggca gtgctatttc tcctgtcact
    10201 atcgaggaca aggaaaccag taagcatatg tttcaggtta tgcgcttgaa agaagaggat
    10261 gaggttacct tagtctttga tgatggcatc aagcgcttgg cgcgcgtgct ggatatggaa
    10321 aatcgtcagt ttgagttggt ccaagaatta aatgacaatg tggaactacc agtccaagtg
    10381 accatcgcat ctggatttcc caagggagac aagctggagt tcatcgctca aaaagtaacc
    10441 gaactgggtg ctagccaaat ctgggccttt cctgcagact ggtcagttgc caagtgggat
    10501 ggcaagaaat tgggtaaaaa agttgaaaaa ctagaaaaaa ttgcccttgg agcagccgag
    10561 caaagcaagc gtaatattgt accaagtatt aaacttttcg agaaaaaagc agattttcta
    10621 gctcagctgg accagtttga ctctatcata gtagcctatg aagaatcagc taaagaagga
    10681 gaagccgctg cgcttctgca agcagtcact ggtcttgaaa caggagccaa actgctcttt
    10741 atctttggtc cagaaggtgg tctgtcacct gcggaaattg aaaattttga agctaaggga
    10801 gctgttttgg caggacttgg ccctcgcatt ctaagagcag aaacagcacc gctttacgct
    10861 ttatcagccc ttagtgtttt agtagaatta gagaaataaa gaggaagaaa atggaacaaa
    10921 aacaccgttc agaatttcca gagaaggaac tctgggactt aacagcccta taccaagacc
    10981 gtgaggattt cttgcgtgca atcgagaaag ctcgcgaaga catcaaccag tttagccgtg
    11041 attacaaggg caatcttcac acttttgagg atttcgagaa ggcctttgcg gaattggaac
    11101 agatctacat tcagatgagc catattggca actatggttt tatgcctcag acgacggact
    11161 atagcaatga cgaatttgcc aatattgccc aagctgggat ggaatttgaa acagatgcca
    11221 gcgtagcctt gaccttcttt gacgatgcct tggtggtagc agacgaggaa gtcttggacc
    11281 gtttgggtga attgcctcac ttgacggcag ctattcgtca ggccaaaatc aaaaaagccc
    11341 actatctagg ggctgatgtg gagaaggcct tagccaatct cggtgaagtt ttctatagtc
    11401 cgcaggacat ttatactaag atgcgagctg gggattttga aatggctgac tttgaagccc
    11461 atggcaagac ctacaaaaac agctttgtca cctatgagaa tttctaccaa aatcacgagg
    11521 atgctgaggt tcgtgagaaa tccttccgtt ccttctcaga gggacttcgt aagcaccaaa
    11581 atacggctgc agcagcctat ctggctcagg tcaagtctga aaaactcttg gctgatatga
    11641 agggatacga ctctgtcttt gattatcttc tagctgaaca agaagtggac cgtgtcatgt
    11701 ttgatcgcca gattgacctc atcatgaagg actttgcacc agtcgctcag agatacctca
    11761 agcatgttgc caaggtaaat ggtcttgaaa agatgacctt tgcagactgg aaattggact
    11821 tggacagcgc cctgaatcct gaagtgacta ttgacgatgc ctatgatttg gtcatgaagt
    11881 cggtagaacc tttggggcaa gaatattgtc aggaagttgc tcgttaccaa gaagagcgct
    11941 gggtggactt tgctgctaac agtggcaagg attccggtgg ttatgcggcg gacccatatc
    12001 gcgtacaccc ttatgtcctc atgagctgga caggccgttt gagcgatgtc tataccttga
    12061 ttcatgaaat cgggcattct ggtcaattca tcttttcaga caatcaccaa agctatttca
    12121 acgcccacat gtcgacctat tatgttgaag caccgtcgac cttcaatgaa ttgctactca
    12181 gtgattactt agagaaccag tctaatgacc cacgtcaaaa acgcttcgct ctggctcatc
    12241 gcttgacaga cacctacttc cataacttta tcacccacct cttggaagca gccttccagc
    12301 gtaaggtgta tacattgatt gaagaaggag aaaccttcgg agcaagcaaa ctcaacagca
    12361 ttatgaagga agttttgaca gatttttggg gcgatgccat tgaaatcgac gacgatgcag
    12421 ctttgacttg gatgcgccaa gctcaatact atatgggctt gtatagttac acttactcag
    12481 caggactagt tatctcgact gctggttacc ttcatcttaa aaactcagaa actggagctg
    12541 aagactggct caatctcctt aaatcaggtg gtagcaagac accacttgag tcagccatga
    12601 ttatcggtgc tgatatttca acagacaaac cactccgtga taccatccaa ttcttgtctg
    12661 acacagttga ccagattatc gcctacagtg ctgagttggg ggagtaaggg gatagagagg
    12721 ttgttataga atgggtatgg acagttattt gcttaaaatt ccatgtttaa atcccccatt
    12781 ccttttcgag gaattctttg ccctgctctg taatggtaag tgaacgtggc gtttgtgcag
    12841 ggatgaccaa ttgtttttca ctgaaaaatt ctaaaagagc attgccgaga gctccgccga
    12901 catgattttt acgttcgctc caatccaaac atttagggca aaatttccgt tttttctttt
    12961 tcaattcatc cgtatcaatg tgtaattttc tttggaaata agacttccca gcttcagtga
    13021 gttggaagtc tttttctgat tcgctgagga gattttcttg caagagataa ttggtaatgg
    13081 aaatgccaag ctcaccagca atatggtcat agcaggtgcg agcgactttt aattcctttt
    13141 tgtgaatgtg ttgtttataa cttttgacat cacgctgagg agagtagaag ctgagaaatt
    13201 ccagcatttc tgcaatggca gcacttttta aagaataata atgaaaacgt ccataggttt
    13261 gtagagtggt tacctgcgct tcggtaagta atttgaggtg atagctgaca gtatgttgct
    13321 tgattttggt aaagctggca atttcgttaa ccgtgtgagc ttttccatcc attagaatat
    13381 ctaaaatgtg catccgagaa gggtctgata agagttttgt gatgatgaaa atatctggtt
    13441 ccataaaaat tttctccaaa tttcatatat cgatgatttt ctatctttt
//
LOCUS       DAVKDZ010000054        13368 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000054 DAVKDZ010000000
VERSION     DAVKDZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13368
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.055"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(155..526)
                     /locus_tag="VWZ30_001525"
     CDS             complement(155..526)
                     /locus_tag="VWZ30_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEU9335232.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQLA"
     gene            complement(585..2018)
                     /gene="glgA"
                     /locus_tag="VWZ30_001526"
     CDS             complement(585..2018)
                     /gene="glgA"
                     /locus_tag="VWZ30_001526"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEU9335233.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGSQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(2015..2821)
                     /gene="glgD"
                     /locus_tag="VWZ30_001527"
     CDS             complement(2015..2821)
                     /gene="glgD"
                     /locus_tag="VWZ30_001527"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR02092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEU9335234.1"
                     /translation="MKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLA
                     KKDISEVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKK
                     EHPEKLRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQ
                     KIYTKVKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLF
                     PRVVIGEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(2766..4154)
                     /locus_tag="VWZ30_001528"
     CDS             complement(2766..4154)
                     /locus_tag="VWZ30_001528"
                     /inference="COORDINATES: protein motif:HMM:NF012398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9335235.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPTMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     SLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHIDNKTQRGPDNFPFLFETFWL
                     KPNGCPQLRCSY"
     gene            complement(4698..6977)
                     /gene="pulA"
                     /locus_tag="VWZ30_001529"
     CDS             complement(4698..6977)
                     /gene="pulA"
                     /locus_tag="VWZ30_001529"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEU9335236.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AVNDAPVWKTFEMKRGNSYSHNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQIIRLKTKTDAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKERLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(7089..9047)
                     /gene="ligA"
                     /locus_tag="VWZ30_001530"
     CDS             complement(7089..9047)
                     /gene="ligA"
                     /locus_tag="VWZ30_001530"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU9335237.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRKLVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQARQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMESLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTIVADAALSGLTVVLTGKLKRLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(9140..10318)
                     /locus_tag="VWZ30_001531"
     CDS             complement(9140..10318)
                     /locus_tag="VWZ30_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9335238.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANV
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(10398..11261)
                     /locus_tag="VWZ30_001532"
     CDS             complement(10398..11261)
                     /locus_tag="VWZ30_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9335239.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFYNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(11264..13165)
                     /locus_tag="VWZ30_001533"
     CDS             complement(11264..13165)
                     /locus_tag="VWZ30_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9335240.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFCQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLVTEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATVEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
BASE COUNT         4039 a         2952 c         2380 g         3997 t
ORIGIN      
        1 tcatagatct gaactttatc ttcataactc aatttcataa taaaacaccc caaaagttag
       61 attttttctg tctaactttt ggggtgcagt tcagttgacc tggggactta taatttgtcc
      121 aactaatttt cctgttaaaa gaccttcttt gacttcaagc tagttggcag tgaaataggc
      181 aataccaagg gattttgcta atctctccaa ctattggtgt aaatccacca gacaccagac
      241 caactaggat gccattcttt tggagaatag agatgaactc tgggacattt agcgatagat
      301 gaattgagtt gaagacgtta tcaaagacca aaataggaag accttccaac aaggacactc
      361 tttttcttaa actgctttca aagaccaact ctcctcgcat tgcccgactt gtaatctgcg
      421 aaatttccgc ctcatgacct gcctctctcc ctaaaagatc aatcacttct tctaggatta
      481 aggttccatc tacatccaaa acacacaagc cttttacttg agacatcagt tctcctctct
      541 aaacagccta aaaatcgtat gaagtcatca tacgatttta tctattaatt aactaaacta
      601 tggtacaagt caaggtatga cttgcaggct gtatcccatg agaagtcact ctccatagct
      661 tgtttttgta ggtttctcca aatgtctgga tggtttctat acaagtccaa tgctgtttgg
      721 aaagtccaat ttaaccaata aggagataga ttgtcaaagc taaagccagt accgcttcct
      781 tcgattggat tgaaagcgcg aactgtatct cgcaagcctc caacttcatg gaccaatggc
      841 aaggttccat aacgcatagc catcatttga gacaagccac acggttcaaa acgacttggc
      901 atgaggaaga ggtcacaagc agcgtagatt tcctgagcaa gtttgacatc aaaagtgata
      961 tttgttgata gcttgtctgg gtaaatctga gcaaaccatg agaaagctcc ttcaaaggct
     1021 ggatcgccag ttcccaaaag aacaatctga acatcttctt gcaagatatg gtgaagactt
     1081 tcgaccacca catcaaaacc tttttgacgt gtcaaacgag aaacaattcc caccagtgga
     1141 acgtctgctc taacaggcaa gccaactctt tcttgcaatt ttgccttatt tttggctttc
     1201 ccagacaaat cttcctgatt gaaatgatag tctaaaagag catccgtctg aggattataa
     1261 agatcagcat caatcccatt cacgatacca gatactttac cagactccat tttaagaatc
     1321 tgatccaaat tacatccaaa ctgactagtc ataatttcat gagcatagct aggtgaaaca
     1381 gttgaaacac ggttcgcata gagaatacct gccttcatcc agttcagaca gttgttccat
     1441 cgaagggtgc catcagcgta acgttcaaag ccaactccaa acaaatcacc caacattcct
     1501 tctgaaaatt gtccttggaa ttctaaatta tgaatggtta aaactgtttc aatgtcctca
     1561 taggcttgaa tccaacggta tttttccttc aacaagaaag gaatcatagc tgtatggtag
     1621 tcatgaacat ggagaagatc aggaataaag tcaatccttt ccatagcctc aatggcagcc
     1681 agttggaaaa aggcaaagcg ttctccgtca tcaaaatcac cgtaaacatg accacggaag
     1741 aaataatatt gattgtcaat aaagtagaag gttacaccat ttaatactgt tttcttaatt
     1801 ccacaatact gtctgcgcca accaacgctc acctcaaaat gaagcacatc ttcaatctga
     1861 cttccaaatt tagcctctac catatcatag tagggtaaaa tcactgcaac ttcgtgccca
     1921 gcttttacca gtgattttgg aagagcgcca atgacgtctc ccaaaccacc tgtttttgaa
     1981 aagggtgcac cctctgctgc tacaaataaa attttcatga atgaatatcc tctgttactt
     2041 tagcaccttt cttaaccaca actggatgtt ctgcagttcc tcgaatcaca acaccaggct
     2101 caacttcaac ccctttgtcc aagatagcat attcgacctg agccccttct ccaataacaa
     2161 cacgagggaa gagcaggcta tctttaacca agctatcctt atggacatga atattacgtg
     2221 atagaacaga attagctact tgaccttcaa taatactacc agaggcaaac tgagaagtgc
     2281 ttaccttaga tgtattagca tagtaagttg gctcttcgtt tttgaccttt gtataaatct
     2341 tttggtttgg tgagaaaaga gaatagaatt tttgtgattc aagcatatcg atattcgctt
     2401 gataataaga tttaacagag tgaatattgg ctagatagcc cgtgtactcg taggcgaaag
     2461 ctccctcttt tacagccaaa tcccgtaaaa catagcgcaa tttctctgga tgttcttttt
     2521 tagcttcttc ttccaagtgt tcaatcaacc aaggtgtatc aacgacaaag atatctgtag
     2581 acatattgaa cgtttcagct gttgacttgc tatcaaagag tttatgagaa agaacatggt
     2641 ctgtttcatc tacatccaag attgcattta cttctgaaat atctttctta gctagttttt
     2701 tataaactac agtgataggc tcttttgttg tactatgtag gtggaaaact tggttcaaat
     2761 caatgttaat aagaacatcg cagttgaggg caaccgtttg gtttgagcca gaacgtttca
     2821 aaaaggaatg ggaaattatc tggaccacgc tgggttttat tatcaatgtg gactgtacct
     2881 acttcaagtt tttcagcaat ttcaaaggct tttttgaaat catttgtaaa gactgatgat
     2941 tgaaggccga attcagattc gttggcaaag gcaatagctt cctctacact agccacacga
     3001 atgattggta aaacaggacc aaatggctct tcccatgcca ctttcatatc ttttgtaact
     3061 tggtcaaaaa gcactggcca gagaagattg ccctcacgtt tgattggtgt aagagcctga
     3121 gcctcttttt cttgtgcatc ctcaatcaag ctccaaatga agtcggctga agcattgtca
     3181 ataacaggtg taatatcagc attgtcaaat ggatcaccaa ctgttaattt agaaacttct
     3241 tcctgaagca aagtagctaa tttatctgct acactttcga gaacaatgac acgtttaatg
     3301 gccgtgcaac gttgtcctga gtagctaaag gctcccgcaa caatttgctt ggcagcatgt
     3361 tccaaatctg catcttctag tacaagagct gcatctttcc caccaagttc caacatggta
     3421 ggacgcatac cagctaaacg accaatacgt tctccaatag gagttgaacc tgtaaagttg
     3481 atgaagttga cttctttgtg ctcaatgata taatccccaa tttctgaacc acgacctgta
     3541 atggtgttga aaacacctgc cggaatccct gcttcttcaa atgctttagc caacaagagt
     3601 ccagaaatgg aaccttgtgt tggtggctta aacatgacca cattccctgc aatcaaggca
     3661 ggtgcaattt tagaagcaga taaattaact ggataattaa agggagcaat cgctagcacg
     3721 ataccaactg gttcacgacg gacaacagcc agtttgtttt tacttgctgc ctcaaaacca
     3781 ccaccttcca ttgcttgtcc agtgatacgg agaccttcct cagcagcata acgaatcaag
     3841 tctgctgtac gcactacttc tccaattgct gctttaatcc cttttgctac ttctttggca
     3901 aggatagtac caatttcttc cttatcgcgt tctaaaatag ctgctgtttt atgtaaataa
     3961 gccgcacgtt caactgctga taaagctcgc catgctggca gggctgcacg cgcagcttgc
     4021 atagcctcat cagcttcagt ctgagtcatg gctggaactg tacccaattc ttcttgattg
     4081 attggtgaat aaatcgtaat ttcttgttca gatgatttcc attttccatt tactaaattc
     4141 tgatatcttg tcaaattctc atcctcctga aaatgattaa gatatatttt accaaatttt
     4201 cagaaaatca caaccctttt tgcaaaattt ttgagattat tttcacaagc ttgatttttc
     4261 aaagtatact caataaaaat aaaaaaaatc cactacgtca aggcaaggct aatgtggttt
     4321 gaagaaattt tcgaagagcg tgaatgagta tcatctatag taaaataaaa aactgaacaa
     4381 tttggttggg gacaaccaaa ccaatttctc acaatgtttt agaaacaagg gtgtgctatt
     4441 ccaatttcag cctactataa ctgtcataga ttgctgaaac aaagtctaga taaaagtctt
     4501 cataataaca agacccccta tccagtgttc aaaaactttg ataggaggtc ttgttttgtg
     4561 aaaatattta tcaaattttc tatacaagtg agctgttagc cagattcttt ctattctttc
     4621 aatttcaata aatggatttt ttaccaatac tcataactgg gaatttgtct gtgtaaaaat
     4681 agcgagatag atggtattta taaaacactc aagacagcta gactaatatc atttaaaaca
     4741 ttatcttctt ttgagcgact gttggttacc aacatagcta aatttcctgc attttcaaat
     4801 tgatagggtt ctgatttagc attcacaacc accaagaggc gttctttgcc gtgaacttca
     4861 tagataaggc agccgctatg ttcaatcgca gaatgcacaa agacatgatg gtaaatttca
     4921 tcatagctag agtaagaaaa ggcatcagtt tttgtcttca atcggatgat ttgacggata
     4981 aactcaatac tgtcttgacg ctcattaatc aagttccagt tcacttggtt cacactgtca
     5041 ggagcattat agctattcat cgcacgctct ctatcatcat gggtcaactc accattttca
     5101 ccagtcgcaa ccagtttggt acgaccaaat tcttgaccga tttccataaa ggccatcccc
     5161 tgcatgagca gattcatggc tgtggcagtt tcgaccttgc gcatgatttg ctctgaactt
     5221 tggtctggat gaagggttgc caataaatcg tgaagattgt aattgtcatg ggcttctaca
     5281 tagttaagca cctgatttgg atgtgtatag cttcctaatt cacgacttcc taggattgct
     5341 ttagctagaa ttggctctgt cgcagcacca ctgacaaaac ctgacttgat agcaccataa
     5401 acttctcccc ctttgacagc atcgcgctga ttgtcattaa agaaaccaat atttggcatc
     5461 tggtaggcat tgtccttctt ggccttatca taaggggcaa gacctgttcc catgtcccat
     5521 ccttctccat agaggataat gttggagtcg atttcatcca agctttgacg aatcatctgc
     5581 atggtcttga catcatgaat ccccatcaag tcaaaacgga agccgtcaat attatattcc
     5641 tgcacccagt atagaagaga atcaatcata tacttgcgaa acatttcgtg ttcactggct
     5701 gtttcatttc caacacccgt tccattctgg aaggtaccat ctggattcat acgataatag
     5761 taatcaggga ctgttgtttg gaatggtgca tcaacaactg agaaggtatg gttatagact
     5821 acatccataa tgactccaat acccgcatcg tgataagctt gaaccatcac cttcaaatca
     5881 cgaatgacct gagctggatc atctggatta gttgaaaaac tagtttctgg cgcgttatag
     5941 ttttgtggat cataacccca gttgtaggtt acatttccat cctcatcgta ttctttatga
     6001 cggtctgcaa ttggttgcaa ttgaacataa ttgtagccca gcttcttgat gtaatcaaaa
     6061 gcagttgact ggccgtattg gttaactgtt ccagcctgag cagcacccaa gaaagttcct
     6121 cgaagatgtt catctacacc cgatgtaggt gatttagtca aatcacgaat gtgcatttca
     6181 cagataactg ccttacatgg attttccaag cgccaagtag cctccgaacc gtgcttaacc
     6241 tcgaagtttt caacttgctt ttctacatgg ctcagaatag ctgaacgttt gccatcaggg
     6301 ctggtcgcga ttgtataagg atcacgtgtc agtgtttggt gatgagggaa ttggacttga
     6361 tactgataag ccttacctac caaatcttcc tcaacatcca aactccagac accgattgta
     6421 ttgtccttat gattatgaga gtagctattg cctcttttca tctcaaaagt cttccaaacg
     6481 ggtgcatcat taacagctga ttcataaacg acaacttgca cttctgtcgc tgtaggtgac
     6541 cagagggaaa aatgagcctg attgtcctct acacggcaac ccaattctcc ttggtaaccc
     6601 caatgatgat caaaactagc actgttaatg gccttatcaa aggcaaaagg attttgattt
     6661 ttatagaaag gactggcaat agcaggattt tcagagtaat aaatcctatc atcgccttcc
     6721 aaaatccaga cctctgttaa taggggatag tgattaaaac ggatagaata ttctttacta
     6781 gtttgacctg tatgaaccac aaaatttaag ctttctataa catgtgaact tgggtgttca
     6841 aagctaaata aagctccaaa ataatcttct ttgtaggtta gcaaatcaat tcgttgatcc
     6901 tgacttttta caaaggagca agtgtcatat tctccattct tacgatggta atgaatgcgc
     6961 atagggtagt tatacatttt ttatttttcc tttttacttt gtttctattt cactaataaa
     7021 tttttgtcaa tctcgtctca attaacagac atagtcatat tctctaaact ctgtttttaa
     7081 acgatccatt acaaactttc tagccatgcc tcatctctga cctggatacc aagttcttgt
     7141 gctttttgca gtttacttcc agcgtctgca cctaccacga cgaggtcggt ctttttagaa
     7201 acactacctg tcactttggc acccagactt tcgagtttac ttttagcttc tgagcgcttg
     7261 agtcgtttca attttcctgt caataccacg gtcaaacctg acaaggccgc atccgctact
     7321 atcgtctgtc ctttatagtc cagattgacc ccagtttctt tcaattctct gagcagaatt
     7381 tcagagcctt ctgtcgcaaa ataagtctga agacttttgg caatcacgcc acctagactt
     7441 tcaatactgg ccacttcctc tgaatctgcc tgagacagat tttcaattga atggaaatat
     7501 tgaagtaaaa gctgactagc cttgcttcct acatgacgaa ttcccaaacc aaataagagc
     7561 ttctcggcag aattttcctt tgatgcttgg atagcctgat acagtttagc agcggacttt
     7621 tccttaaccc cctctaaaag gaggaaatcc tcttcttgca aacgataaat atccgccaca
     7681 tccttgacta aattagcagc aaagagcttc tcaacaatag atggaccaag gcctgtaata
     7741 ttcatagcat cacgagaagc aaagtgaatc aagctttcca tgatttgagc agggcaacgc
     7801 ggattgatac aacgtagggc cacttcatct tcaaagtgca acaagtcaga gttacaactt
     7861 ggacagtttg tagggatatc tagtttttct tcagaaaccc gtttggactc taccacacgt
     7921 aaaacggcag ggatgatatc accagcctta tatacaatga ccgtatcgtc ttttcggata
     7981 tctttttcag caatataatc tacattgtgc agggtcgcac ggctaacagt cgtaccggca
     8041 agttgtactg gtgttagatt agcagttgga gttacaacac cggtacggcc aactgtccag
     8101 tcaactgata agagttgagc ttctttttct tcagcaggga acttgtaggc tactgcccac
     8161 tttggagcct taactgtaaa accaagttct tcttgacttg ctaggtcgtt gaccttgatt
     8221 accactccat caatatcgta aggcagattt tcccgttcct gtcctacttc ttggataaaa
     8281 ttccagattt catctatgtt ttcagccaag attcgcttag gattgaccac aaaacctagt
     8341 tgttctaggt acttcaaccc cttttcttgg ctatcacgag ttgaagggct ggcttcttga
     8401 tagagaaacg ttgcaagatt acgcttggca actactgctg tatccaactg acgcagagtt
     8461 cctgctgccg cattacgagg attagcaaat tcaggctctc cattttcttg gcgcgcttgg
     8521 ttaacttggt caaaggaagc gcgtggcatg taacattccc cacgaactgt gatatctagt
     8581 tcttctggca aagtcaaagg gatgtcctta acacgcttga ggttttctgt gatattttca
     8641 ccaattgaac catctccacg tgttacccca gcaaccaaaa tccccttttc ataagtcagc
     8701 gagatagata agccatcgat tttcagctca caaatatagg tcggatgagc cacttcctta
     8761 cgaacacgcg catcaaaagc atctagctcc tcacgtgaaa aagcatcctg caaactataa
     8821 agaggatact gatgactgta tttttcaaaa ccatctaaaa ccttgccacc aacacgatga
     8881 gtcggactgt ctgctagcac ttgctctgga taagcagttt ctaactcgac caacttacgg
     8941 taaaggcggt catactcact gtctgaaacc gagggattat cgctggtata gtactcagtc
     9001 gcatagcgat tgagcaaagc gactaactca ttcattcttt tattcataag accattttac
     9061 cataaaacaa accctcctca caaacgagaa gggcggaaaa aacacttagt ttgaaattat
     9121 ttttgaaact caagcaacct tatatcaatt tttcaaaatg agttcgaaca taaataaacg
     9181 atatacaaga caagatgata acaccacttc caattatcag gaaagaagag agatgtacac
     9241 ttggcaagac tgtcataaat ccttttgcaa taggcataaa tagaatagct aaggtaaaaa
     9301 ttgtactcag tactctccca agaaattcgc tctcaacctt ggtttgtact tgagtaaaaa
     9361 agtgaatatt aaaaatcgtc ataaacaatt cacaaactaa atttccagaa aaggaaagaa
     9421 aagttggaag tggtaatccc atcataaaaa ctccgacacc tgtcaaagcc agtaaaatca
     9481 aaagattata aacattagct ttaattttac tagctagaag agccccaatg atggaaccaa
     9541 tagcccccat agttaaaata cttgcatagg ctccttctga cccgtaaagc tgattcgaaa
     9601 agggaagtag aaattcaaaa gctgcaaaaa agaaattaac gctggaagct accagcaaaa
     9661 ggaagaaaat ttcttgctga tgccagatat agtgtaaccc atccttgata tctacaaaaa
     9721 tatctctccc agtaaaagcc tttttctctt gaacttttgc ttcctctttt ggaaggaaag
     9781 ccactagaac aaaagcaatg aaaaaagtca gcgagtctag cagtagcgtc atatggagac
     9841 ttgcaaactg taaaacaagg aaggaaagaa caggagagct gacacctaca acctgcaaaa
     9901 ccagctctag gcgagaatta tagatcacaa tctcatcttt ctccaccact tcagttatga
     9961 tagctttatt ggctgtgcga gaaaaggcaa aagcaatagc ctgcacaatg ttagcaacaa
    10021 tcaaagcgcc aatcatccag ctatcattcc ttatgaaaga aatagccaga caaagaatcc
    10081 cacaaacaag atctgccgtc attaaaatct tacgacgaga aaaacggtct gaaataactc
    10141 cgccaaaggg attgacgaga atagatgtga cgagctcaga aatctgatac attcctaaaa
    10201 ctgtctgtcc tatagtcccc atagaagcca accagacact atttccataa tcatagagca
    10261 tatttcccat tttattgata gccccacggc taatcaactg cactgcatag cgattcatat
    10321 taaagctcct ctcaaatttt gaaactattg tatcaaaacc gaaaggagct ttttattttt
    10381 tcccttattt gggaaaatta acttttgaca aatttttcgt agtgttcctg ataataggct
    10441 acttgctccg gaagacctaa cacatcaaaa atatgcatgg cctcttgcat ctgcttacag
    10501 ccttctttac actgtccttt ttgatataag gcaaaacctt ttaaataatg gaaaacatta
    10561 cgctcataaa gcttaatacc tttgtcaata atcttctctg tataagcctc aaaatagttg
    10621 gcattataaa aagaagaatg ctctaaacaa tgctggtaac aatttagggc caaaatcaaa
    10681 actaatctct tatggcgact aatctcttgg taaaattcct ccctctccat aacttctcta
    10741 ccaatccgag tgacatagtc tacatcgtag aaactataga ggttaccgaa aagaatcaac
    10801 tcatacatgg tccattcttc tgttttgaag agataatctg ctaccttatc caaatcatcc
    10861 tgcttcatat cataactcgc atctctttga caaatcagac cttgtagcaa aatccagttc
    10921 agctcaaaat aaaggggagt cgtcgaactc ttagactttt caagttgttc tctttgaagc
    10981 ttttgaaaac ctgcaatatc gtttgaatag taaagtggga taatctgtgc catcatagac
    11041 acatgttcat gattataaaa attccttgcc ttatccatga aattttcgat tgttacatga
    11101 atattatcca aaatctcaaa gaaacgggag actgccaggt cagactcccc aagctcaaaa
    11161 cgagataact gagaggtaga gcaggattcg cctgctgctt cctttaaaga ataatttcca
    11221 cttgttcgaa attcacgaaa tacttttcca agatgttcca tttttacacc tgctctgata
    11281 attcttccca ctcaagcata gcttcttcct gacgatggct gattttgtcc agctcagcct
    11341 gtaattccat gagtttgtcg gcatcgtttg tttccaacat ttgttcagaa atggcttggc
    11401 tttgactttc tagctcttca atttcagctt ctagactttc gatttgtcgc atgagtttgc
    11461 gaacttcttt ttgactttct ttctgggcct gatagtcatt gactggactt gcttcctttg
    11521 cttgattgct agttgaagct tcctcagtct gactcatttc tactgttgct ttcttttcaa
    11581 catagtagtc gtaatctcca aggtagagag ttgaaccatt ctcagacaat tccaaaacat
    11641 gagttgccac acgattgata aagtaacgat catgactgac aaaaagcaag gtaccatcaa
    11701 agtcaatcaa ggcattttct agcacttcct tactatcaat atccaagtgg ttggtcggct
    11761 catccagaat caaaaagtta ttgttttcca tagacaattt agctaaaagc aaacgagctt
    11821 tttcgccacc agatagcatg ccgactgatt ttttaacatc atctcctgag aaaaggaagg
    11881 ctccaagacg gttgcggatt tcaacttctg gtgtcagttt gaaatcattc cagagttcat
    11941 ccagcaccgt attacttggt gtcagcttgc tttgggtttg gtcatagtaa ccaacctcaa
    12001 cattagcgcc aaagcgcttt tctcccttga taaaaggaat ctggtccaca atagacttga
    12061 taaaggttga cttgccgata ccatttggac caacgatagc gacagcattc atcttacgaa
    12121 gatctaggtt gataggttgt gacaagactt ccccgtcata accaatagca gtattttcaa
    12181 cagtcaaaac aacattgccc gacgtttttt cagactggaa ggtcatgttg gctgctttct
    12241 taccagcttc aggcttgtcc aaacgctcca ttttttctag ttgtttacgg cgagattgag
    12301 cacgtttagt cgttgaagca cgaactagat tgcgattgac aaagtcttcc agagcagcga
    12361 tttccttctg ttgcttttca tagttttttg cctcagtaac tagcttttgc tccttcaatt
    12421 cgacaaaacg agagtaattc cccacatagc gatccaagga atgcttggtc aaatctagcg
    12481 taattgtcgc aaccttgtcc aagaaataac ggtcgtggct gacgataatg agggcaccgc
    12541 tatagtttac caagtaattc tctagccagg cgatggtttc aatatccaag tggttagttg
    12601 gctcgtccaa gaccaagaga ttgggctttt caaggagcat tttggcaagt gccaaacgag
    12661 tattttgacc accagaaagc tcagcaattt tcatctgcca catagactcg tcaaacttga
    12721 atccattcaa aatcgctcga atatcagctt cataggtaaa gccacctgct tggcaaaaat
    12781 tctcagataa gcggtcataa tctgacatca gtttatccaa atcctcacca gacttttcac
    12841 ccatctccag ctccatctga cgcagttgtc tctccgtccg acgcaaatca ttaaagacat
    12901 gaagcatttc atcgtagatg gtattttcag actcaaaacg gctatcttgg gctaggtaag
    12961 acagagaaat atcttttttc ttattgattt ctccgctagt tggctcttct tctccaacta
    13021 aaatcttcaa aagagtagac ttacctgcac catttttccc aacaagagca atccgatctc
    13081 gttcatcaac ctgcaggttg atattatcga aaagaacctc tcctgcaaaa gaacgttcaa
    13141 ttttattagc ttgtaaaata atcataagag tagtatagca tgtttcccta aggcattcaa
    13201 gataatcgta agtcttttag tacaactttt ataacataaa ataaactaaa ttatgtatat
    13261 tttatattag attccttcac tatcttgttg gattttctaa ccagctaatc ttgtttcaaa
    13321 tagttatcgc acaagtctat tatttaattc ttttcatcat ttacgtac
//
LOCUS       DAVKDZ010000055        12778 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000055 DAVKDZ010000000
VERSION     DAVKDZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12778
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.056"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(751..1611)
                     /locus_tag="VWZ30_001534"
     CDS             complement(751..1611)
                     /locus_tag="VWZ30_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335241.1"
                     /translation="MVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQEYCQGQPY
                     GLLKKMLKRIRLSREISLLRATIIVYFFTHRILPLSVFTFMFSYILLFVQSYLGSNTA
                     WIGNRRLIIEDEFEKILLSKSYIKEISSARYSEYLTCEYKNLTPIILLAIFENLLDSY
                     LLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYDNEALRDLS
                     KQYLNKNISELQDGSFESGIQDAVASKQIVVINEFIACLNSRCVPSQYDRFFYKDRPY
                     IFSRKSPIKG"
     gene            complement(1560..1805)
                     /locus_tag="VWZ30_001535"
                     /pseudo
     CDS             complement(1560..1805)
                     /locus_tag="VWZ30_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2686..2898)
                     /locus_tag="VWZ30_001536"
     CDS             complement(2686..2898)
                     /locus_tag="VWZ30_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335242.1"
                     /translation="MLIVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKKLQTK"
     gene            complement(2906..3217)
                     /locus_tag="VWZ30_001537"
     CDS             complement(2906..3217)
                     /locus_tag="VWZ30_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335243.1"
                     /translation="MGGAIYIPRWTITGAITGAAYAALAAEGGGGLQLVLASYGLRSA
                     LVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGADWIFTNIIDGWDGRRDNQLRI
                     G"
     gene            complement(3201..3278)
                     /locus_tag="VWZ30_001538"
                     /pseudo
     CDS             complement(3201..3278)
                     /locus_tag="VWZ30_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
     gene            complement(3611..3916)
                     /locus_tag="VWZ30_001539"
     CDS             complement(3611..3916)
                     /locus_tag="VWZ30_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPAR17_0115 family bacteriocin-like peptide"
                     /protein_id="HEU9335244.1"
                     /translation="MELVLPNNYVDLEQEEMMYLDGGEPMSISAIVALAITIGGASYS
                     TGYVLGERVYYSGYKNSDYQKNKWGIRGAMLVAFGAVGAIVMLGFENRYYALVTGRG"
     gene            complement(4062..5403)
                     /locus_tag="VWZ30_001540"
                     /pseudo
     CDS             complement(4062..5403)
                     /locus_tag="VWZ30_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865356.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
     gene            complement(5400..5867)
                     /locus_tag="VWZ30_001541"
     CDS             complement(5400..5867)
                     /locus_tag="VWZ30_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9335245.1"
                     /translation="MAKLEVKDNKKLVLKSVICKKLHDTKVEDVDQEINKFHQHLQLL
                     KAQIFGPLIVKSCGTTIHDDGLITTDFEFYIQAHNAQQYSNIYDVQDSISVPYCLYVR
                     FEDSPEYLQYAYSKLDLYVYENDIQTDGIVYTVYVNSSPEKMVVDIFRPIVSL"
     gene            complement(5860..8079)
                     /locus_tag="VWZ30_001542"
     CDS             complement(5860..8079)
                     /locus_tag="VWZ30_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEU9335246.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     RNSKFYVADPASGIRKMSSDELGEIYQGITLFMVPNSDFERGKLKGKGLLDLFGRLIF
                     NQKGLISTVILASFVLSIIGILSSLFSKVIMDEVIPYALKNSLYMFLIVFGIVSFLQT
                     LLSAFRQHVLLFLSRKIDIPVLMGYYDHIIHLPYSFFGSRRVGDVLTRFQDAMTIKNV
                     FTSVSISLVMDITLSVISAVVLWTINQSLFLILVFMVIVNIILIYCFKKPYKKINHEQ
                     MEANGLLNSQLIESIRNIDTIKSQHDEEQRLNKIEEKFVHTLEIGYKEGVLQNIQSTI
                     SSMTSTMGGLLFMGVGALFIIDGKMTIGDLLVFQTLSQYFTEPIQNLVGLQLTFQEVQ
                     VAVSRLQELMEVDREDIALDYSIRDFTLCDDIEFKDVTFAYGSRPPVIEDFNLRIKQG
                     EKIAFVGESGAGKSTLVRLLLRFINPSEGKIRIGENDLSDLDYGKLRKKISYIPQTIE
                     LFTGTIIDNLKIGNPSVTYEDMVRVCRIVGIHDTIQRLQNRYGSFVEEGGQNFSGGEK
                     QRLAIARALLSKADLYIFDEATSNLDSFSEQIIQDLIFNKIMDKTTIVVAHRLSTILR
                     CDKICFLENGTIMEYGTHEELMAKNGKYARMVGLQSVQVNQQIQSQAVLDTEEVTYG"
     gene            8545..9408
                     /locus_tag="VWZ30_001543"
     CDS             8545..9408
                     /locus_tag="VWZ30_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9335247.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPMEEIRDMLEIVIYLRQHGAGELSEHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFHIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYTQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDNAKLIQKGFSLLELTEETSILSHLEK
                     EVEIYYQAKER"
     gene            complement(9460..10047)
                     /locus_tag="VWZ30_001544"
     CDS             complement(9460..10047)
                     /locus_tag="VWZ30_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9335248.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVGQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            10289..10960
                     /gene="sdaAB"
                     /locus_tag="VWZ30_001545"
     CDS             10289..10960
                     /gene="sdaAB"
                     /locus_tag="VWZ30_001545"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU9335249.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPDIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNVHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            10969..11841
                     /gene="sdaAA"
                     /locus_tag="VWZ30_001546"
     CDS             10969..11841
                     /gene="sdaAA"
                     /locus_tag="VWZ30_001546"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU9335250.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            11863..12495
                     /locus_tag="VWZ30_001547"
     CDS             11863..12495
                     /locus_tag="VWZ30_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9335251.1"
                     /translation="MISITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLIYQPDYIAHKPLEVLAYFQ"
     gene            12545..12772
                     /locus_tag="VWZ30_001548"
     CDS             12545..12772
                     /locus_tag="VWZ30_001548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335252.1"
                     /translation="MKIKEQTRKLAAGCSKHCFKVVDRTDEVSSKHCFEVVDRTDEVS
                     NHIYGKATLTWFEEIFEEYKSLHNKTHNIRC"
BASE COUNT         4130 a         2564 c         1943 g         4141 t
ORIGIN      
        1 ccccgtaaat atttgtcaag tgttttatat tacatttttg ttacaaattt ctatatcata
       61 agcttctact cccaaaaata gataaagata ctacttttgc ccttttcaat tctcttttta
      121 aacatagtat catcttcctt cattcctata aaaacatttc cgattgagag agcttgtttt
      181 ttaatttcgt taaacaacaa aaaacgcaag ctcaagtctg cggacaatct ttatagccat
      241 ttcatttctt tcacatctgt tttacgaact ttagcatatt tcttagcgct tgtaaaatgt
      301 agagtaactg gtttaaatac tccgcttttg tctcttcgta aacctaaaac tgcggatctt
      361 ttatttctat cctcaaaaaa tatgacgtcc aatttcatag tatttttaga taaatcctta
      421 gctacgatgc aacaacttgt tattttagaa tagaaaatat caccgataaa ctcatataaa
      481 gatatccttg cacattgcat cacctaatcc tgcgctactt gcccataatc ctttaatgtt
      541 tctactaaaa ttcttccaat ctcccctatc aagatagctt atttatccta gacaatgcct
      601 actgcaacag tataaataaa agtcatccgc catcttttct ggcaatcata tgggtagcct
      661 aatgatattt ctagttactc aaaaatatct agctatacta gagttatttc taatccctag
      721 gacgcttgtt aatccaaata taacaaattt tcacccctta ataggactct ttctggaaaa
      781 aatataaggt ctatctttat aaaaaaatct atcatattgg gatggcacgc atctggaatt
      841 taaacaagct ataaattcat ttataactac tatttgttta gaggcaacag catcttgtat
      901 accgctctcg aaagaaccat cttgtaattc cgaaatattt ttgtttaaat attgttttga
      961 caagtctctt aatgcttcat tatcgtaaat gattcccaaa aatccaacct tatatagtaa
     1021 ataatttaaa cttacaaagt aattgaatcc catagtatat ttttctttat aaagcaaagg
     1081 taaaacttta taaaaaatat ctaagtcaac ctcactctga ttctgaagca agtaggagtc
     1141 aagcaaattt tcaaaaatag ctaacaatat gattggtgta aggtttttgt attcacatgt
     1201 caagtattct gagtatcgtg cagatgatat ttcttttata taactttttg aaagcaagat
     1261 tttttcaaat tcatcctcta ttattaatct tctatttcca atccaagcgg tattgctacc
     1321 caaataagat tgtacaaata gcaatatata agaaaacatg aacgtaaata ctgaaagagg
     1381 tagaattcta tgcgtaaaaa aatagacgat aatggtagcc cggagtaaag atatctctcg
     1441 tgacaatcga attcttttca gcatcttttt taacagtccg taaggttgtc cttggcaata
     1501 ttcttgaact aaattaatcg gaatgacatc tctcaccatt tctggaaagt agagtaagtt
     1561 tataggttga aatctccagc tcccatcata agtaaatgga tatagaacca aatacttaaa
     1621 agattttttt cttataaact taattatcac aatatgaatg actgttccaa tgaagtatat
     1681 taaatataaa agtaaaacca ccatcaatat acttacaaaa atccccttgg aatcaatgga
     1741 aaataaaata ttgcaattag aagcgaataa agtcccgcta ataaatattt ctgatcatct
     1801 ttcattcaaa acaccatatt atttactaag aatgcatcaa tacaatagag caactctgta
     1861 agtcaatatt tgataaagca aagctttaaa cccttcccca gacgttagct tttattcaac
     1921 ccactacagt tgacaaggag cccaaattat tgataaaaac tttagtcatc atcgtctcta
     1981 taaaaatatt ttctatacac tcttgataat accaaaatta atgccaatat cataccaaga
     2041 gctataataa tagaatttat tagatttatt ttagtatatc caatcactag tccaataatt
     2101 ggactagcag acatgcttag atataataaa aataacaaaa aatgaatttc accaattcct
     2161 gcaaaaaaat gtttcatagc tatagtttta cgcccataaa tacccatcaa atttaccgtc
     2221 tacgatatct aaaccatatg caatggctcc tcctaatgag aagacttcag caacagtagc
     2281 cgcaatatta agagcattag taatagttgc catactaact ccaccaatat atcttgttat
     2341 agcacttagt tacctagata agatatcctt tagcttgctt ccaaaattta agtctacacc
     2401 tataatatga gttcatatta gtactgctaa atcctatcta tagtaatgtc aaacacctaa
     2461 atcaagtcat ctctccaatt cattcaagaa atgaagaaag gcaaaaaatc tttaaaataa
     2521 gaaaagtgca tattatcagg tgttcaaaaa tcttgataat atgcgttata taatagaaaa
     2581 atttactcaa tttcattttc aaattgagtt ttttcccagc ctcattttta atttatatct
     2641 tcaatagaat ctaatgaccg agctactgta ttacacagat taatattatt tagtctgtaa
     2701 tttttttaaa aaacttaata gtaaatttgc tccactgatt gctatcaata tttcaattgg
     2761 taaaaaatag taattccaaa taaaatgaga aatcagcaat gatagaaaaa taacaatatc
     2821 aattttcctg ttgttgacat actctgtaat tttatgatac aggaatgcaa caataactga
     2881 aattaaaaca atcaacataa gattattaac ctattctcaa ttgattatca cgtcgcccat
     2941 cccagccatc aataatattg gtaaaaatcc aatcagctcc agcaccaatt ccagcagatg
     3001 caatacttct aataacagta tttgcaaaag catttccaat atgaattcct aatactccta
     3061 aacctttaac aatcccagct accagtgcgg agcgtaatcc ataagatgca agaactagtt
     3121 gaaggcctcc acctccttct gctgctaatg ctgcatatgc tgcgccagta atggctcctg
     3181 taattgtcca cctgggtata tatatagccc cccccatcaa ggtacatcat ctcttcttca
     3241 tcaatcacaa cataattatt tggtaataca agttccattt gttttctcct ttttattaag
     3301 catgcttagg atggtttcct aacaagtgat cgcaggcttt gtttagttac ctaaattaga
     3361 cttccttcct ttatcttatt ttaaatattt aaaaccagtt actgcttgaa gcttaacagt
     3421 ttacttaatt caaataacag ctaatcactg attccctgat tttatcaaat atactaataa
     3481 caacaattgt gatacgatca tcattataag aacaacattt aacgaatcaa agtttttccg
     3541 agataataat aaagcaacag caagcagtat tattactaaa gattgaataa ctactattac
     3601 tcttaacatt ttatcctctg ccagtaacca aagcatagta tctattttca aaaccgagca
     3661 taactattgc acctactgct ccaaatgcaa ccagcattgc tcccctaata ccccacttat
     3721 ttttttgata atcagagttt ttatatcctg aatagtagac tctttctcct aacacatatc
     3781 ctgtgctata cgatgcgcca ccaattgtga tagccaaggc aacaatggcc gaaattgaca
     3841 ttggttctcc gccatcaaga tacatcatct cttcttgttc aagatcaaca taattatttg
     3901 gtaatacgag ttccatttat tttctccttt tttattaagt atggttagga tggtttccta
     3961 acaagtaatc gtcgactgtg tttctatcac ttagctacct agataagata tcctttctct
     4021 accttatttg tttttatagg ttacctaagg tttaaaacca attattgctt gaaacttaac
     4081 atttctaaaa tccaatcaag ataggtttct ttatcataga caatccgcac ttcaactggc
     4141 atatcctttt ggaggacgac tgtctcactt ccatgtttta gagttaaggt ttctaattat
     4201 atcatgactt tataaagact aatattgcct tctttcgttt cttgggaaat tgttcctgaa
     4261 tcaatctgtc tgacttgtcc ctttagtgtt ccatattttt gactatttac accagtaata
     4321 gcaacatcag cttttgctcc tttcgaaaca cgagaaatat cactcgcaag tacaaaagct
     4381 tctacatagt aacctttttc tttccctgaa acttctgcta tcgtttgccc ctgctgaata
     4441 gacattcctt gtttcaaagg taacagataa tgcagagtac cttcatttgg cgctaatata
     4501 ctatgagctt tatcttgacc tgttgccact ccaagattag cctcaagctc tgtaatactt
     4561 ttattgttgt ttgaacgagc agtacctaac tcactaacta attgagcata aatattataa
     4621 gcctgagaag ttggatcatt ataggtacgg ttcatatcct taatattgct ttgttgacta
     4681 tcaatctcgc tattcttaga agtaatattt gtttttaatg cttcgatttt tgttgttatt
     4741 tccgatatct tagtttctaa cttagctttg ttatcagctg atgtcgtatc tgatagagaa
     4801 ggtgagtctc cttgctgttg aagttgaagc aattcatttt gtagagcttg caagtcggca
     4861 tctaactgat ttctttcagc tgttatttta ttcaacttcg catattcatc ctgaatcttt
     4921 gaatactggg taccattatt ataattttcc gagtttaact gagacagata gtattcaact
     4981 ttcccataat attcctattc ttcaccagaa ttagataaat gattgtattt ctcatttaat
     5041 gattgttcaa atttttgaaa gatagcttct tttgcacgta acttctccaa ttgtttattt
     5101 aaagagctcg cttgtaaacc ttcttgtcca cttgataacg aaataatgac atctcctatc
     5161 tccttctgac ttatgtaaag taactacctt ccctgaacca attgctgata ggaactctgt
     5221 acctgttata actgaatttc cattcgcttt tacaatatag tttttgggta tataagctgc
     5281 gccaaccaat gcaccgctta agataatagc agttgaaata atgagaataa acgcaaaagc
     5341 tggtggtctc ttatcaaaga aaatacgaga ataacgtaat tctgatttat tatataattt
     5401 cataggctta caattggtct aaaaatatct actaccattt tttcaggaga agaattaaca
     5461 taaactgtat agacaatccc atccgtttga atatcatttt catagacata tagatccaat
     5521 ttagaatacg catactgtag atactctgga ctgtcttcaa aacgaacata taaacaatat
     5581 ggaacagaga tagaatcctg tacatcataa atgttactgt actgttgagc attatgagct
     5641 tgaatataaa actcaaaatc agtcgttatt aatccatcat catgaatagt agtaccacaa
     5701 ctttttacaa ttaatggacc aaaaatttgt gcttttaaca actgcaaatg ttgatgaaat
     5761 ttattaattt cctgatctac atcttctact tttgtatcat gtaacttttt acagataact
     5821 gactttagta ccagtttttt attatctttt acctctaact tagccataag taacctcctc
     5881 tgtatctaac acagcctgtg actgaatttg ttgattcact tgaacgctct gcaaaccaac
     5941 cattctagca tactttccat ttttcgccat tagttcttca tgagtaccat attccataat
     6001 agtcccattt tctaaaaagc atatcttatc acatcgaaga attgtagaca gcctgtgggc
     6061 tactacaatt gttgtcttat ccattatttt attaaagatt aaatcctgaa taatctgttc
     6121 actaaatgaa tctaagttag aggttgcctc atcaaatata tacaaatcag ctttactcag
     6181 tagtgctctt gcaatagcca atcgttgttt ctctccaccc gagaaatttt gaccgccttc
     6241 ttctacaaag gagccataac gattttgaag acgttgaatc gtatcatgaa taccaacaat
     6301 acgacaaact ctcaccatat cctcatatgt aacagaagga ttaccaattt ttagattatc
     6361 aataattgtc cctgtaaaga gttctatggt ctgaggaatg taggaaattt tcttacgtag
     6421 tttaccatag tcaagatctg ataagtcatt ttctcctata cgaattttcc cctcactcgg
     6481 atttataaat cttaataaca aacgaactag cgtacttttt cctgctccgc tttctccaac
     6541 aaaagctatt ttttctcctt gtttaattcg caaattaaag tcctcgataa caggagggcg
     6601 tgaaccatag gcaaatgtta catctttaaa ttcaatgtca tcacataagg tgaagtctct
     6661 aatactataa tccaaggcaa tgtcttcacg atctacttcc atcaattctt gaagccgact
     6721 aaccgcaacc tgtacctctt gaaaggttaa ctgcaagcca actaaattct gaataggttc
     6781 tgtaaaatat tgacttaatg tctgaaaaac caagaggtcc ccaatagtca tttttccatc
     6841 aatgataaat aaggcgccca ctcccataaa taataaccca cccatggttg aggtcattga
     6901 agatattgta gattgaatat tctgcaaaac accctcttta taaccaattt ctaaagtatg
     6961 aacaaatttt tcttcaattt tgtttaaacg ttgttcctca tcatgctgag acttaatggt
     7021 atcaatgtta cgaatagatt caattaactg tgaattaaga agtccatttg cttccatttg
     7081 ttcatgatta atttttttat aaggcttttt aaaacagtat ataagaatga tgttaacaat
     7141 aaccataaaa actaatatca gaaataaaga ttgattaata gtccaaagga caaccgcact
     7201 aataactgac aaggtgatat ccatgactaa tgaaatagag actgacgtaa acacattctt
     7261 tatcgtcatc gcatcttgaa atcttgttaa aacatcacca actctacgac ttccaaaaaa
     7321 actataagga agatgaataa tatggtcata atatcccatt aaaaccggta tatcaatttt
     7381 tcgagataga aacaaaagaa cgtgctgacg aaatgcagat agaagagtct gtaagaacga
     7441 tactattcca aatactatca aaaacatata cagactattt tttaaagcat atggaatcac
     7501 ctcgtccatg ataacttttg aaaagagact cgataaaata ccaatgatac ttaaaacaaa
     7561 ggaagctaat atgactgtac taatcaatcc tttttgatta aaaattaacc ttccaaataa
     7621 atcaagaagt ccttttccct ttagtttccc tctttcaaaa tccgaatttg gaaccataaa
     7681 aagagtaata ccttgataaa tttctcccag ttcgtcactt gacatcttgc gtatgccact
     7741 agcagggtct gctacgtaaa atttactatt ccttttgata ctatgtaata ccacaaaatg
     7801 attttgacct aaatcattct taacttgaag aatcgcagga aatgttaatt ttggagtcaa
     7861 atcttccagt gctactcgaa cagcttttac tgtaaaattc aacttattca gacctgaaac
     7921 aataccactg acagtcgttc catacatgtc tgtaccaata atttccctaa tcttcatgat
     7981 tgatagttcc tttttgtaag ataaaagaac tgttgaaaca actgccgcag cacaatctga
     8041 ctggtcatgt tgcatcgtac aaggatattt ttttaacata gaaatactca attctttaga
     8101 attcatctaa cttttctttg atgtcaccca aacctcaatc actgcccagt agttcttgaa
     8161 ttagtttatt ttcaaccgta tcattctttg ttcttgatat ctttccaagc attgtagtac
     8221 agcagatttt tgtccaagga ctaagcctcc tttaacaact caagctccct tacattatat
     8281 cggccagccc catggaaaga ttatagccag actaatcaat gatataatac atactagaat
     8341 acaaaatatt tttttattca tactttttct cctatcttta ttttctacct acatagtacc
     8401 tcttattaag ctcatttcaa aattacgtta acttttttca caaatttatt tttactttct
     8461 tttttacttt ttacactgct atgatacaat atttttaaca agattttatt atttgttaac
     8521 ttttttcaca gaaaggagaa aatcatgcgt tatgatttcg gaaaggtcta taaagaaata
     8581 cgtgagtcaa aggggctgac ccaagaagag gtctgtgggg gtgttctctc aagaaccagc
     8641 ttatcaaaaa tcgaaagtgg caagacaacc ccaaaatatg aaaatatgga atttcttctc
     8701 cgtcaaatca atatgagttt tgaagagttt gaatatatct gtcaacttta tcaaccaagt
     8761 caacgcacag agattatgca aacctatctc aatatgcgtt ccattatcgg gaccagcgat
     8821 cttgtcaatt tatttcaaaa atgccaagac tacctcaaga cccaccacga tctccctatg
     8881 gaagaaatca gagatatgct tgaaattgtt atttatctcc gtcaacacgg agcaggggaa
     8941 ctatcagaac acgcagaaca ggttgtcaaa aaactttgga aaaaaattga aaaacaagat
     9001 acatggtatg agagtgatct aaaaatcctc aataccattc ttttcagttt tcacattgaa
     9061 tacctccatc tcatcactgg aaaaatattg caacgcctag aagtctataa aaactatcaa
     9121 catttatatg acttgcgaat gacaattcta ttaaaccttt ccacactcta tctatacact
     9181 caagataaaa acatgtgtaa gcaaatctgc tacactttac tagaggacgc caagaataag
     9241 aaaagctacg ataggcttgc tatctgctat gtccgtattg ggatttgtac agataatgct
     9301 aaacttatcc aaaaagggtt ctcccttctg gagctaaccg aggaaacttc tatactgtct
     9361 catctcgaaa aagaagtaga gatctattac caagcgaagg aaagataaaa aagccgaggg
     9421 atttcctcga ctctttacat attatttgtt cgttagttct taataccagc cattgttaag
     9481 ccagaagttt ttagcagcag tccatgaacc gtaacgtcct gcaacgtagg catctgctac
     9541 acgttcttgg ttttcagctg agtagtcacc gttcaggtat gaatctgtta attggtaacg
     9601 tccgatataa cgtccatttg tagctgtata gctaccacct gattcttttt gagcgatcca
     9661 ttctttggct tctgcttcag atccgcttac agttgaaaca actgtttcac ttactactgg
     9721 agtttctgct actggagctg aaacagtttc atcttgagcg gctggtgccg cataagtagc
     9781 tggcgctggt gttgcaacag gcgctacagg gccatcgata accaactctt gaccaacata
     9841 aatcaaatga atgttatcaa tgtggttgtt ttctgccaat ttttcaactg ttgtgttgtg
     9901 agtttcagcg atttctgaaa gtgtatcacc ttctttaaca gtgtaagttg atgattcttg
     9961 agcagataca aatgatggag caaatactgc aaacaaggca gctactcctg caagagttgc
    10021 tttaatcttt ttagttattg atttcatatt cgaaaattct ccttctttct atagttctat
    10081 catacccttt aaatattacc gtttcttgac acttttatgt agaaatatta caaaattatt
    10141 ttttatttcc ctagataagg tgttttttgt cataaaagat actattttta tgctatttga
    10201 ggtattagaa aggttttcat ccacaattaa agtcaagacc ctcattcttt gatataatag
    10261 agataagaat ttttataagg ggaaactgat gaaatcactt cgttttcaat ctgtctttga
    10321 tatcatcgga ccagttatga ttggcccatc tagtagccat accgctggtg ctgttcgtat
    10381 tgggaagatt gtctcttcca tttttgatga tactccgaca gaagttgaat tccaactttt
    10441 taactcattt gccaagacct atcgtggtca cggaacagac ctagcccttg ttgcaggtat
    10501 tttaggcatg gatacagatg atcctgacat tccaaacagt ctggagattg cccacaagcg
    10561 tggtatcaag attgtctgga ccattcagaa agacagcaat gcccctcacc ccaacaccac
    10621 taaaattacc gtcaaaaatg tccacaagac catcagcgtg actggtattt ctatcggtgg
    10681 aggaaatatt caggtaaccg aactcaatgg ttttgccgtc tctctcaata tgaatacacc
    10741 aactatcatc atcgttcatc aagatattcc aggcatgatt gccctcgtaa cagaggcgct
    10801 ttcccgctat ggtatcaata tcgcccagat gaatgtcact cgtgaaaaag ctggtgaaaa
    10861 agccattatg attatcgaag ttgacagtcg caactgtgat gaggctatcg aagaaattcg
    10921 aaaaatccct catctccaca atgtcaattt ctttaaatag gaggaagcat gttttattct
    10981 atcaaagaat tggtcgagca agcagatctg gactttcaag gaaatgtcgc agaactcatg
    11041 attacaacag agtttgaatt gaccggtcgc gaacgtgaag aagtcttcct tctcatggaa
    11101 cgcaatctgg aagtcatgaa agcctctgtc caacttggcc tcaatgaaaa taaatctcgt
    11161 agtggcctga caggtggaga tgctgccaaa ttggaccact acatcaaaaa cggaaaaact
    11221 ctgtcagatt acacgattct ctctgctgcc cgaaatgcta tcgcagtcaa tgaacacaat
    11281 gctaaaatgg gcttggtctg cgctactcct accgcaggaa gtgccggttg tctcccttcc
    11341 gttctcactg ctgctattga aaaattagac ctcagccacg agcaacaatt ggatttcctc
    11401 tttgctgctg gtgcctttgg actagtcatc gcaaacaatg cctccatctc aggtgctgaa
    11461 ggtggctgtc aagctgaggt tggttcggcc tctgctatga gtgctgctgc cttgactctg
    11521 gctgcaggtg gaacacctta tcaggccagt caagctattg cctttgtcat taaaaatatg
    11581 ctaggcctca tctgtgaccc tgttgcaggt ttggtcgaag ttccctgtgt caaacgtaat
    11641 gccatgggag ctagctttgc tttcatcgca gcagacatgg ccttggcagg tatcgaatct
    11701 aaaatccctg tggatgaagt gatcgatgcc atgtaccaag taggagcaag catgccaact
    11761 gcctttcgtg aaacagctga aggtggactc gctaccaccc ctactggtcg tcgcctccaa
    11821 aaagaaattt tcggagaata agcttatcaa ataggagaaa ccatgatctc tatcacagcg
    11881 atttttttcg atttggatgg aaccctcgtt gatagttcta tcgggattca caatgccttt
    11941 acctatacct ttaaagagct gggggtgcct agccctgatg ccaaaactat tcgtggtttt
    12001 atgggaccac ctctcgaaag tagttttgcg acctgcctgt ccaaagacca aatttctgaa
    12061 gccgtgcaga tatatcgttc ttactataag gcaaaaggta tctatgaagc tcaactcttt
    12121 cctcagatta tagacttgct tgaggagtta tcaagcagtt atccactcta catcaccacg
    12181 acaaagaata catctactgc tcaagacatg gcaaaaaact tggaaatcca tcatttcttt
    12241 gatggtattt atggttctag ccctgaagca ccccataagg cagatgtcat tcaccaagcc
    12301 ttgcagacac atcaactagc accagaacaa gccatcatca tcggagatac caagtttgat
    12361 atgctgggag ctcgagaaac aggtattcag aaattggccg tcacttgggg atttggagag
    12421 caagcagact tgctaatcta tcaacctgat tatatcgccc acaaaccctt agaagttttg
    12481 gcgtattttc aatagcatag actgggtaac aactccattt cagaagaaaa tgaagtaata
    12541 ctcaatgaaa atcaaagagc aaactaggaa actagctgcg ggctgctcaa aacactgttt
    12601 taaggttgtg gatagaactg acgaagtcag ctcaaaacac tgttttgagg ttgtggatag
    12661 aactgacgaa gtcagtaacc atatctacgg caaggcgacg ttgacgtggt ttgaagagat
    12721 tttcgaagag tataagtctc tacataacaa aacgcataat atcaggtgtt gatgaact
//
LOCUS       DAVKDZ010000056        12746 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000056 DAVKDZ010000000
VERSION     DAVKDZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12746
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.057"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(102..2360)
                     /locus_tag="VWZ30_001549"
     CDS             complement(102..2360)
                     /locus_tag="VWZ30_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9335253.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGFQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILRGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPIGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADINKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2584..2814)
                     /locus_tag="VWZ30_001550"
     CDS             complement(2584..2814)
                     /locus_tag="VWZ30_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HEU9335254.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVSYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3004..3687
                     /locus_tag="VWZ30_001551"
     CDS             3004..3687
                     /locus_tag="VWZ30_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9335255.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQVGILGVDLSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAIKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTIYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KRMGQGDKK"
     gene            3687..4421
                     /locus_tag="VWZ30_001552"
     CDS             3687..4421
                     /locus_tag="VWZ30_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9335256.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESKAVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4575..5432
                     /locus_tag="VWZ30_001553"
     CDS             4575..5432
                     /locus_tag="VWZ30_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU9335257.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTEKLKEETGLVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGFRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALWTLDRK"
     gene            5574..6446
                     /locus_tag="VWZ30_001554"
     CDS             5574..6446
                     /locus_tag="VWZ30_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU9335258.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLPSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            6728..7411
                     /gene="rpiA"
                     /locus_tag="VWZ30_001555"
     CDS             6728..7411
                     /gene="rpiA"
                     /locus_tag="VWZ30_001555"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU9335259.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7425..8636
                     /locus_tag="VWZ30_001556"
     CDS             7425..8636
                     /locus_tag="VWZ30_001556"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU9335260.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8638..9180
                     /locus_tag="VWZ30_001557"
     CDS             8638..9180
                     /locus_tag="VWZ30_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEU9335261.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVENLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPLY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9196..10005
                     /locus_tag="VWZ30_001558"
     CDS             9196..10005
                     /locus_tag="VWZ30_001558"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9335262.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHAGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10502..12109
                     /locus_tag="VWZ30_001559"
                     /pseudo
     CDS             10502..12109
                     /locus_tag="VWZ30_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664829.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            12134..12646
                     /locus_tag="VWZ30_001560"
     CDS             12134..12646
                     /locus_tag="VWZ30_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEU9335263.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
BASE COUNT         3771 a         2319 c         2895 g         3761 t
ORIGIN      
        1 tatatatcat aatggacaaa gggtaagctg tcaagaaatt acagttattt tagtaaaaaa
       61 gaagcagaaa aatttcattt tctactcctt ttctatagga tttatttttc tttttcagaa
      121 cttttaactt cagctttttt ggcagattta atctgaatgt ttcccttgct agtcataact
      181 gctttgagat ctttttcgct tggattttca aggtagtagt cagtgattgt gtcctcaata
      241 tagtcttgaa tagtccgacg aagtgggcgt gctcccattt ttggatcata acctaggtca
      301 accaactttt ccttgacctt gtcagttaca tccaaacgaa tgttgttgct agagaggcgc
      361 ttgttaatat ctgctagcat gagctcgaca atctgaagga ggttatcctt gctgagagcc
      421 ttaaattcga taatgccatc aaaacggttc ataaactctg ggctaaagaa gttaccgagt
      481 tcaccgagga cagaattggt acgtccttct ctagcagcac caaatccaac gctagcttcg
      541 gtctttcctg tacctgcatt tgaggtcatg ataatgatgg catccttgaa gctaacggtg
      601 cgtccttgcc cgtctgtcaa acgaccatcg tccaagactt gaagaaacat gtgcataaca
      661 tctgggtgag ctttttccac ttcatcgaga aggatgagag aatatggatt gtggcgaact
      721 ttttcagtta attgaccagc ctcatcatag ccaacataac ctggaggagc gccgaccaac
      781 ttagccacac tatgtttttc catgtattca ctcatatcaa agcgaatcat actatcagca
      841 gaaccaaaaa gttcgatagc cagttgtttg gaaagttctg tcttaccgac accagttggc
      901 ccaacgaaga ggaagcttcc gattgggcgg ttaggggtac caagtccgac acgattacgg
      961 cgaatagcct tggcaatctt atcgactgca tcatcttggc caataacatg agacttgaga
     1021 tcttcggcta gatggatgag ttgagattgt tctttctctt tcaaatcacc aatagggata
     1081 ttggttttct gctcgataat gtgctcaata gttttctcgc tgatgatagg agtatcctgg
     1141 tctgtgatct ttttcttttg catttcctta tacttggcaa tctggtcgcg gaagtaggcc
     1201 gccttctcaa aatcttcttc tcgtgtagct tgagacttga gattttcagc ctcaatcaag
     1261 cgctgatcaa ttactttagg atccacaaaa ttcaaggtca agttcatctt agaaccagct
     1321 tcatctagga ggtcaatggc cttgtcaggc aagaagcgat cttggatgta gcgattggaa
     1381 agagttgcag ctgcttcaat cgcagcatcg gtatattgaa cgtggtggta atcttcgtat
     1441 ttcttttgaa tccctctgag aatagtgatt gtttcgtcca ccgttggttc atcgacttta
     1501 acaggctgca tacgacgctc gagggcagca tccttttcaa tgatacggta ttcattgagg
     1561 gtagtagcac cgactagttg cagttctcca cgagcaaggg ctggcttgag gatatttcct
     1621 gcgtccatat taccatcact cgcagaacca gcaccaacaa tttcatggat ttcatcgata
     1681 aagaggatga tgtcttcacg tttgcgaatt tcttccatga gtttttgcat gcgttcttca
     1741 aattgtcctc gaatccccgt tccttgaact aagctaacca catccagacg gatgacttgt
     1801 ttaccttgga gtttatgtgg cacatcgcca tcgacaattt tctgagctag accttcgaca
     1861 acggccgttt ttccgacacc aggttcaccg ataaggacag gattattctt ggttctacga
     1921 ttgagaatct cgatgacacg gataatctca tcgtcgcgcc caataacggg gtcaatgtct
     1981 ccacgacggg caatttcagt tacattaata ccaaattctt ccagcaggcc tttttcttgg
     2041 ctaggtggcg gagtttgagc agatccacga ttttggaaac cataaccgcc gtttccaccg
     2101 taacctccac ctgattgggt tgggggaata ggaggagtat tgctagaagg tctgaaattg
     2161 tttagatcat tgaagaaatc accaaaggga tcgaagtcac gattgttcag atccgtcata
     2221 cctttgaaga ggctattgtt aggatctgtt ttgataatct tatagcagtt ttgacagagg
     2281 tcaatttgtt tttgttttcc attgagattg gtgtaaagat gaattgttga gtcgttaatt
     2341 ttacagtttt gacaaagcat acatctacct catttctttc tttagcctga cttgtttaaa
     2401 ggtcaaaaat agtcaaattc tatactctca ttataacagg attggggtgt aaagcaagta
     2461 aaaagattga agctttgaga agttgttaca atgaaaattt ttgtgaattt ggactataaa
     2521 aaaatcctat ttgtgagaca aaaaggattt tacttagaca tgcagggaca atcccagctt
     2581 gttttagtag agaagttcgt aaatttccat tgcgattaag tcaatgctat caaacgtata
     2641 cgtttcgcga tcttctacgt cacgaagggt aaagattggg ccgttttctt cgtcgtggct
     2701 gtacgaaact tctgcaacaa caactccttc gcgttcaaaa ttacgtttta aatttccgcc
     2761 atcgtttgcc attgcttcaa ggcggttgat gattctaacc aaatgtgatt ccattttgat
     2821 tctccttcat ttcttatcgt tactatttta ccataaagga ggccaatttg actagaaaaa
     2881 actaagattt cttgctaatt ctgctagttt aaagattttt aaataaattg gataaaagat
     2941 ttgaatttta attcaaaaca tgttataatc atacagtatt ctattttaga aagcagtgtg
     3001 actatgaatt tttctttttt acctaagtat ttaccttatt ttaactatgg ggctgttgtg
     3061 acgattctta tttctatctg tgttatcttt ttgggaacta ttttgggtgt tgtcttggct
     3121 tttgggcaac gttcaaagtt taaaccgctt gtttggttgg ccaacttgta cgtttggatt
     3181 ttccgtggga caccgatgat ggttcaaatt atgattgcct ttgctcttat gcatatcaat
     3241 gctccgacta ttcaggttgg aattttaggt gttgatcttt ctcgtctgat tccagggatt
     3301 ttgattatct ctatgaacag tggtgcttat gtttctgaga ctgttcgtgc cggaatcaat
     3361 gcagttccta aggggcagtt agaggcggct tattctttag ggattcgtcc taaaaatgct
     3421 atgcgctatg taattttacc acaagctatc aaaaatatct tgccagcttt ggggaacgaa
     3481 tttatcacca ttatcaagga cagctccctc ttatcagcta tcggtgttat ggagttgtgg
     3541 aacggggcta caacagtttc tacaacaatc tatctacctt taacaccact tttatttgca
     3601 gctttttatt acttgattat gacttctatt ttgacagtag ccttgaaagc ttttgaaaaa
     3661 cgtatgggac aaggagataa gaaataatga cagaaacctt gataaaaatt gaaaatttac
     3721 ataaatcatt tggaaagaat gaagtattga agggcatcaa cctcgagatt aaaagaggag
     3781 aagttgtcgt tatcatcggt ccttcaggga gcgggaaatc taccttgctt cgctctatga
     3841 atttgttgga agaagcgacc aagggtaagg ttatctttga gggagtcgat attacggaca
     3901 agaagaatga cctctttgcc atgcgtgaga agatgggcat ggttttccaa caatttaacc
     3961 tctttcctaa tatgactgtg atggaaaata tcaccttgtc acctatcaag accaaaggtg
     4021 agagcaaggc tgttgctgag aagagagccc aagagctttt ggaaaaagtt ggtttgccag
     4081 ataaggcaga tgcttatcca cagagtttgt caggtggcca gcaacaacgg attgccattg
     4141 cgcgtgggtt ggccatggaa cctgatgttt tgctctttga cgagccaact tcagcactag
     4201 atcctgagat ggttggagaa gttctggctg ttatgcaaga tctagccaag tcaggaatga
     4261 ccatggttat cgtaacacat gagatgggat ttgcccgtga ggtggcagat cgtgtcatct
     4321 ttatggcaga cggtgtggtt gttgaagacg gaacaccaga acagattttt gaacaaaccc
     4381 aagaacaacg gactaaagac ttcttgagta aggttttata atctatttta aaatttcaag
     4441 acaagattag tgaaaagctc aaccagagct ttttcttata gtttgaaact ataggattgc
     4501 ctaggaaaga agtgttagag ggtgctggca gtttttggta taatggaaga tatttgaaag
     4561 aaaagagaag tgatatgaca cagattattg atggaaaagc tttagcggcc aaattgcagg
     4621 ggcagttggc tgaaaagact gaaaaattaa aggaagaaac aggtctagtg cctggtttgg
     4681 tagtgatttt ggttggggac aatccagcca gccaagtcta cgttcgcaac aaggagaggt
     4741 cagctcttgc agctggtttc cgtagcgaag tagtgcgagt tccagagacc attacgcaag
     4801 aggaattgtt agacctgatt gctaaatata atcaggatcc tgcttggcat gggattttgg
     4861 tccagttgcc attaccaaaa cacattgatg aagaagcggt tctattggct attgacccag
     4921 aaaaggatgt ggatggtttc catcccctaa acatggggcg tctctggtct ggtcatccag
     4981 tcatgattcc atcaacacct gcaggaatta tggaaatgtt tcatgaatat gggattgact
     5041 tggaaggtaa aaatgcggtc gtcatcggcc gctccaatat cgttggaaag cccatggccc
     5101 agcttctttt ggccaaaaat gcgacagtaa ccttgactca ctcacggact cataatcttg
     5161 ccaaggtggc tgctaaggca gatattcttg tggttgcaat tggtcgtgct aagtttgtga
     5221 ctgctgactt tgtgaaacca ggtgcggtag tcattgacgt tggaatgaac cgcgatgaaa
     5281 atggtaagct ctgtggggat gtggattatg aggcggttgc cccacttgct agccatatta
     5341 cgccagttcc tggaggtgta ggtcctatga ccattactat gctgatggag caaacttatc
     5401 aggcggcact ttggacattg gatagaaaat aagagaaaca ttcgattaat agagtgtatt
     5461 ttctatagct atatctaaaa tggcagaaat gaatattaaa ttttagatat aagattacaa
     5521 aaggaggtct gcgcctcctt tttgttgtat aataaaagtg agaggaaaaa aggatgaaag
     5581 tgattaatca aaccttacta gaaaaagtta ttattgaacg ttctcgttca agtcataaag
     5641 gagattatgg tcgtctgctg ctgcttggtg gtacttatcc ttatggaggt gccatcatca
     5701 tggctgcttt agcagctgta aaaagcggtg caggattggt gaccgttgga acggacaggg
     5761 aaaatatccc ggctctgcac agccatttac ctgagcctat ggccttttct cttcaagacc
     5821 agcaattgtt aaaagagcaa ttggagaagg cagaagttgt cttgctgggg cctggtttac
     5881 gagacgatgc ttctggagaa aatctagtaa aacaggtctt tgttaattta agccaaaatc
     5941 agattttgat tgtagacgga ggtgccttga ccattcttgc taggacaagt ttgtcatttc
     6001 cctcaagcca gcttatccta actcctcacc aaaaagaatg ggaaaaatta tctggaatta
     6061 cgattgaaaa gcaaaaggaa gatgcaacgg ctagtgttct gccttctttc cctcaaggaa
     6121 caattttggt ggagaaaggc ccagctactc gtatttggga agttggccaa tctgattatt
     6181 accagttaca ggttggtggt ccttatcagg cgactggggg aatgggagat actctggctg
     6241 ggatgatcgc aggatttgta ggccaattcc gacaggccag tctctacgag cgtgtggcag
     6301 tagcgaccca tcttcattca gccatagctc aagaactatc tcaagaaaat tatgtggtct
     6361 taccgacgga aatcagcagg tatcttccca aaataatgaa aattatttgt caacaagaga
     6421 gggtaagcaa agataaactt gtttaaaata gacagataaa tctacttcct tctcttaatt
     6481 tatgattctt gaaatgcacc tgctttaagg ctaaactaga cgtatcaaag ttaagagttt
     6541 gtttttgtaa ctggaaagag atgaacaaat caaatacata aaagataaac tcatttttat
     6601 tcgtttggta aaaataaaat acatattttt aaagaaagat tgatgaaaaa tgtagatttg
     6661 atgtttttac ttgaataaaa agcggtttca tagtaaaata agaactgaga atgatggagg
     6721 taaatcggtg gaaaatctga agaaaatggc aggtatcaag gctgctgagt tcgtgagcga
     6781 tggaatggtc gttggacttg gaacaggctc gactgcctat tattttgtcg aagaaatcgg
     6841 tcgtcgtatc aaggaagaag gattgcagat tacagctgta acaacttcta gtgtgaccac
     6901 taaacaggca gaaggcctta atatcccgct caagtctatt gaccaagtag actttgtcga
     6961 tgtgacggtt gatggcgcgg atgaagtgga tagtcagttt aatggaatta aaggcggtgg
     7021 tggtgccctt ctcatggaaa aggtggtcgc aacaccatca aaagaataca tttgggtggt
     7081 ggatgaaagc aagctggtcg aaaaactagg tgcttttaaa ttgccagtag aagtggttca
     7141 gtatggtgca gagcaggtct ttcgtcattt tgaacgagct ggctacaaac caagtttccg
     7201 tgaaaaagac ggccaacgtt ttgtgacgga tatgcagaat tttatcattg acctcgcctt
     7261 ggatgtcatt gaaaatccaa ttgcttttgg acaagaattg gaccatgtcg ttggtgttgt
     7321 ggagcatggt ttattcaatc aaatggtgga taaggtaatc gttgctggac gagatggagt
     7381 tcagatttca acttcaaaaa aaggaaaata gaagggggca taagatgtct aaatttaatc
     7441 gtattcattt ggtggtactg gattctgtag gaatcggtgc agcaccagat gctaataact
     7501 ttgtcaatgc aggggttcca gatggagctt ctgacacact gggacacatt tcaaaaacag
     7561 ttggtttgaa tgtcccaaac atggctaaaa taggtcttgg aaatattcct cgtgaaactc
     7621 ctcttaagac tgtagcagct gaaagcaatc caactggata tgcaacaaaa ttagaggaag
     7681 tatctcttgg taaggatact atgactggac actgggaaat catgggactc aacattactg
     7741 agcctttcga tactttctgg aacggattcc cagaagaaat cctgacaaaa atcgaagaat
     7801 tctcaggacg caaggttatt cgtgaagcca acaaacctta ttcaggaacg gctgttatcg
     7861 atgattttgg accacgtcag atggaaactg gagagttgat tatctatact tcagctgacc
     7921 ctgttttgca gattgctgcc cacgaagaca ttattccttt ggatgaattg taccgtatct
     7981 gtgaatacgc tcgttcgatt acccttgagc gtcctgccct tcttggtcgc atcattgctc
     8041 gcccttatgt aggtgaacca ggtaacttca ctcgtacggc aaaccgtcgt gacttggctg
     8101 tatctccatt ttccccaact gttttggata aattgaatga ggctggtatc gatacttatg
     8161 ctgtgggtaa aatcaacgat atctttaacg gtgctggtat caaccatgac atgggtcaca
     8221 acaagtcaaa tagtcatgga attgatacac tattgaagac tatgggactt gctgagtttg
     8281 aaaaaggatt ctcattcaca aacctagttg actttgatgc cctttacggc catcgtcgta
     8341 atgctcacgg ttaccgtgat tgcttgcatg agtttgatga acgcttacct gaaattatcg
     8401 cagctatgag agagaatgac cttctcttga ttactgcgga ccatggaaat gacccaacgt
     8461 atgcaggaac ggatcacact cgggaatata ttccattgtt ggcctatagc cctgccttta
     8521 aaggaaatgg tctcattcca gtaggacatt ttgcagatat ttcagcgact gttgccgata
     8581 actttggtgt ggaaactgct atgattgggg aaagtttctt agataaattg gtataagatg
     8641 acgcgctatg ctttgctggt gagaggtatc aatgttggtg gtaagaataa ggtcgtcatg
     8701 gcggagcttc gtcaagaatt gacaaactta ggactggaaa aggttgagag ctacatcaac
     8761 agtggcaata ttttctttac ttcgatagat tccaaagccc aattggttga aaagctagag
     8821 actttctttg cagtccatta tccatttatt cagagctttt ctttactgag tctagaggac
     8881 tttgaggcgg aacttgaaaa tctaccagct tggtggagca gagacttggc acgaaaagat
     8941 tttctctttt acactgaggg tttggatgtg gaccaagtca tcgcgacagt tgaaaattta
     9001 gagctgaaag atgaagtgct ttattttgga aaacttggga ttttctgggg gaaattttct
     9061 gaagaatcct attctaagac tgcctatcat aagtacttgc tgaaggtgcc tttataccgc
     9121 cacattacta ttcgtaatgc taaaaccttt gacaaaattg gtcaaatgct aaaaaaataa
     9181 taaaggagac acacaatgac atttttaaac aaaatccatg aaactgctac tttcctgaaa
     9241 gaaaagggaa ttgcagcccc tgagttcggt ctaatccttg ggtcaggact tggagaattg
     9301 gcagaagaaa tcgaaaatcc agttgtagta gactatgctg agattccaaa ctggggccgt
     9361 tcaacagtag tcggtcatgc tggtaaattg gtatatggtg aactggcagg tcgcaaggtc
     9421 ttggctcttc aaggtcgttt ccatttctat gaagggaatc ctctggaagt ggtgactttc
     9481 ccagttcgtg tgatgaaagt tcttggatgt gaaggtgtta ttgtaaccaa tgcagctggc
     9541 ggtatcggat ttggtcctgg taccttgatg gctatctcag accatatcaa catgacgggg
     9601 caaaatccat tgatgggtga aaacttggat gactttggcc cacgtttccc agatatgtct
     9661 agggcctaca caccagaata ccgtgccact gcccatgaag tggctaaaaa acttaatatc
     9721 aagcttgatg aaggtgtcta tatcggagtt actggtccga cttatgaaac accagcagaa
     9781 attcgttcct ataagacact gggagcagat gcagttggta tgtctacggt tcctgaagtt
     9841 atcgtggcag cccacgctgg cttgaaagtt ctgggaattt catgtatcac taactttgcg
     9901 gctggtttcc aagaagaact caatcacgaa gaagttgtag aagtgactga acgtgttaaa
     9961 ggtgatttca aaggcttgct taaagcgatt cttgctgaat tgtaagaaaa aagatttaaa
    10021 agggggagtg cctctgtttt ttcaggattg actgcctatc cggattaaag aagaaacaga
    10081 ggaatactat gagcttcttc ctgctcttat aactgaaaga agcggaagaa taggtatgtc
    10141 tgatctgata gccagcattg tgaaagacaa gattctagga tactagcatt agcttcctag
    10201 ccaagcagac tagtatgata aggagagatg agaatgaatt gactttctga atttctcagt
    10261 cttatcatat atagcacaat gagatttcgc ttgagtctgc ttgtaaataa acgaaaagaa
    10321 agataagaaa taatgaaaat tggtcaacga attatgcgct ttggcataaa aaattaagta
    10381 tcggagttgt atctgttgta gtcggctttg atttctagct ccagctggaa tttcagccaa
    10441 tgaagtaaag ttagatgtaa catctgaagt ggtaataggt gtgctagatt ctaaggagga
    10501 attgaaagag tcagaaaatg atgctccaaa actagaaact cctcttagag aggagccaag
    10561 actagctcct caaacgcttc cggaagcaag tgaagttctt gaaaacaaaa gggaagagtc
    10621 aaaagtagag ataacagagc cagctcaaga ctcacctatt cttgctcctg tagaagaaac
    10681 taaagaagaa gctgtgacag aaaaaccaac aaatactcgt tctctaactg cagaagattt
    10741 ggtgaagatt tccaaagggg aattgcattt agaaaatgat ttgattgatg aatctttcta
    10801 tggtgaaaaa gctcttgatt tggaagggga tgatgaccag gatggcatca aaaacaaaga
    10861 tggtaaggat tatctaggat ataacagtca tcccttgcta gcagacagtg atggggatgg
    10921 tttggcagat ggggaagatg ataataagaa agaatggtat gtcacagacc gtgattctct
    10981 tctctttatg gagttagctt atcgagacga tgattatatt gagaaaattt tagatcataa
    11041 gaatcttttc cctagtctct atcttgaccg tcaagaacac aaactcatgc acaatgaatt
    11101 ggctcctttc tagaagatga aaaaagccta ctatacagat agtggcttgg atgctttctt
    11161 atttgagacc aagagcgacc ttccttatct caaagatgga acggtgcaca tgttggctat
    11221 tcgtggaacg cgagttaatg acgccaagga cttgagtgca gatttggttt tattaggtgg
    11281 aaataaacta gctcaagcgg atgatatccg caaggttgtt ggggaattag ccaaggatat
    11341 aagtattact aagttgtata tgacaggtca ttctcttgga tgctacctag ctcagattgc
    11401 agaggttgaa gcttaccaaa aatatcctga tttttataac catgtattga ggaaagtgac
    11461 aactttcagt gctcctaaag tgattacttc cagaactatt tggaatgcta agaatggttt
    11521 ctgggatgtt ggtttggaaa gtcgtaaatt agctgttagt ggaaaaatta agcattatgt
    11581 ggttgataat gacaatgttg tgactccctt gattcataat aatcgtgata ttgttacatt
    11641 tacaggtaat tcacgcttta aacaccgttc tcgtggctat tttgaaagtc caatgaatga
    11701 tattcctaac tttaatattg gtaaacaagc taccttggat aaacatggtt atcgtgatcc
    11761 gaaattggat aaagtgcgat tctttaagaa acaggctctg cctcgatctt ctagtcaacc
    11821 aagcgctgaa ccaatggaaa atattgcctt aggaaaacag gttactcaaa gttcgacagc
    11881 tttcggagga gatgctagaa gagctgtgga tggcaaagtc gatggtaact atggtcacaa
    11941 ttctgtcact catacaaact tccaatctaa gccttggtgg caagtagatt tggctaaaga
    12001 agaaaccatt cgccaaatca atatttacaa ccgaacagac actgcccagg atagattggc
    12061 aaactttgat gtcattcttt tagacagttc tggtaaagaa attgagtgaa aacgtataat
    12121 atctcctaaa gatgtgtcag cacaaattac gattaaccat aaaaaagcgc gctatgttcg
    12181 gattgagcta gaaggctata atgccctcag tcttgcagaa gttgaagttt tccgctttat
    12241 agctacgaat gctgaaacgg cgacacaagt ttctaagcca gttcaaccaa tcagtcagac
    12301 tcctgtgaaa gataaaacat tgacaattca acacagtgga gcttacattg cccgctactc
    12361 cataacttgg gaagaagttc cagtagataa agatggaaac caagttgttc gtagtcattc
    12421 ttgggaagga aacggtcgca accagactgc aggttttgtc ctcaacctcc caatcaaaga
    12481 aaatatgaga aatctgcgag ttaagattga gaaaaagacg ggcctactat ggaatagatg
    12541 gcaaacaatc tatgaaaaca gaccaatttt agctcaaccc caccgtaaaa ttacccattg
    12601 gggtacgaca ttgaattcca aggtgagtga cgatgatgtc ttgtaatctg atggtagaat
    12661 gacagttagt ttgtctagtt tataagaaag tactacctga gcttgaatag aactcaggta
    12721 gctctctatg aaagaacaaa attaat
//
LOCUS       DAVKDZ010000057        12322 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000057 DAVKDZ010000000
VERSION     DAVKDZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12322
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.058"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            117..3218
                     /locus_tag="VWZ30_001561"
     CDS             117..3218
                     /locus_tag="VWZ30_001561"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU9335264.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRVAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKECQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGF
                     SLGKADILRRATGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            3301..4308
                     /gene="pfkA"
                     /locus_tag="VWZ30_001562"
     CDS             3301..4308
                     /gene="pfkA"
                     /locus_tag="VWZ30_001562"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU9335265.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4367..5872
                     /gene="pyk"
                     /locus_tag="VWZ30_001563"
     CDS             4367..5872
                     /gene="pyk"
                     /locus_tag="VWZ30_001563"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU9335266.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATNSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6557..6782)
                     /locus_tag="VWZ30_001564"
                     /pseudo
     CDS             complement(6557..6782)
                     /locus_tag="VWZ30_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7630..8502
                     /locus_tag="VWZ30_001565"
     CDS             7630..8502
                     /locus_tag="VWZ30_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEU9335267.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQYATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8648..8992)
                     /locus_tag="VWZ30_001566"
                     /pseudo
     CDS             complement(8648..8992)
                     /locus_tag="VWZ30_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9135..9446
                     /locus_tag="VWZ30_001567"
     CDS             9135..9446
                     /locus_tag="VWZ30_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335268.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETAEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKYSI
                     K"
     gene            9450..10166
                     /locus_tag="VWZ30_001568"
     CDS             9450..10166
                     /locus_tag="VWZ30_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9335269.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIISDLALLISKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQVPKEIASLMNEWLAYFL"
     gene            complement(10295..11110)
                     /locus_tag="VWZ30_001569"
     CDS             complement(10295..11110)
                     /locus_tag="VWZ30_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEU9335270.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKRVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHDNSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(11107..12012)
                     /locus_tag="VWZ30_001570"
     CDS             complement(11107..12012)
                     /locus_tag="VWZ30_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU9335271.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PEKVYHFLPRNRWGRIFFGTFVGMLFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
BASE COUNT         3858 a         2151 c         2617 g         3696 t
ORIGIN      
        1 caaaaataca aatgaataga aaataaatta gtaagctgat gaaatttttc tcaagagaag
       61 ccatttatag gtgaaaatgg tataatatag tgagaaggat agaggagaag tgtaaattga
      121 tcgcacaact agatacaaaa acagtctata gttttatgga aagcgtcatt tcgatcgaaa
      181 agtatgtgag agtagctaaa gaatacggct acactcattt ggctatgatg gatattgaca
      241 atctttatgg cgctttcgac tttctagaga ttacaaaaaa atacggcatt catcctttgc
      301 tagggcttga aatgacagtg tttgtagatg atcaggaagt gaatttgcgc tttttagctc
      361 tatctagtgt gggctatcag cagttgatga agctttcgac agccaagatg cagggggaga
      421 aaacttggtc agtcctgtcc cagtacctgg aagatatcgc ggtcattgtg ccttattttg
      481 atagagttga gtcgttagaa ctaggctgtg attactatat aggggtttat ccagaaacac
      541 tagcaagcga atttcatcat cctatcttac ctctttatcg ggtcaacgct tttgaaagca
      601 gggatagaga agttcttcaa gttttaacag cgattaaaga aaatctaccg ctcagagaag
      661 ttcccttgcg ttcgagacaa gatgtcttta tatcagcaag ttctttagag aaactattcc
      721 aagagcgttt tccgcaagct ttggacaatt tagaaaagct tatttcaggc atttcttacg
      781 acttggatac tagtctgaaa ctgcctcgtt ttaatccagc tagaccagca gtagaggagt
      841 tgagagagcg tgctgaactg gggcttgttc agaaggggtt gactagtaaa gaatgtcaag
      901 atagactaga ccaagaattg tctgttattc atgatatggg ctttgatgat tatttcttgg
      961 ttgtttggga tttgttgcgt tttggacgat cgaatggcta ttatatggga atgggaaggg
     1021 gttctgcagt aggcagtttg gtttcttatg ccttagacat cacggggatt gacccagtag
     1081 agaaaaatct gatttttgaa cgctttctta atcgtgaacg ctataccatg cctgatattg
     1141 atattgatat cccagatatt tatcgtccag attttatcag atatgttggt aataaatatg
     1201 gtagtaaaca tgcggcacaa atcgttactt tttcaacctt tggagccaag caagctcttc
     1261 gagatgtctt gaaacgcttt ggtgtgccag agtatgaatt atctgcaatt actaagaaaa
     1321 tcagttttcg tgacaatctt aagtcagcct atgagggcaa tctccagttt cgtcagcaaa
     1381 tcaatagtaa gttagaatac caaaaagctt ttgagattgc ttgcaagata gagggctatc
     1441 caaggcaaac ctctgtccat gcggctggtg ttgtaattag tgaccaagat ttaaccaact
     1501 acattcctct aaagtatggt gatgaaattc cactgactca gtatgatgct catggagttg
     1561 aggctagcgg acttttgaag atggactttc tgggactacg aaatttgacc tttgtccaga
     1621 agatgcaaga gttgcttgct gaaacagaag gtattcatct gaaaattgaa gaaatcgatt
     1681 tagaagacaa agaaacgtta gctttatttg cctctggtaa tacaaaaggt atctttcaat
     1741 ttgagcaacc aggtgccatt cgtctgctta agcgtgtgca accagtctgt tttgaagatg
     1801 tcgtcgcgac tacttctcta aatcgaccgg gtgctagtga ctatatcaat aattttgtgg
     1861 caagaaagca tgggcaggaa gaagtgactg ttctggatcc agtactggag gatattttgg
     1921 ctccaaccta cggcatcatg ctctatcagg agcaggttat gcaggttgcc cagcgacttg
     1981 ccggatttag tcttgggaaa gccgatattt tgcgtcgggc gacggggaaa aaggatgcct
     2041 ctgccatgca tgagatgagg gcttccttta ttcaaggttc attagaagct ggtcatactg
     2101 tggaaaaagc agagcaggtc tttgatgtta tggagaagtt tgcaggttat ggttttaaca
     2161 ggtcacacgc ctatgcctac tcagccttgg ccttccagtt ggcttatttc aaaacgcatt
     2221 atccagccat tttttatcag gtcatgttaa attcttccaa cagtgattac ttaatagatg
     2281 cacttgaagc aggttttgaa gtagcctctc tatccatcaa caccattccc tatcacgata
     2341 aaattgccaa caaggccatc tatctaggtt tgaaatccat taaaggagtc agtaatgatt
     2401 tagctctctg gattattgaa aatagacctt attctaacat tgaagatttt atagctaaat
     2461 tacctgagaa ttatctgaaa cttcctctgc tagaaccttt ggtaaaagtt ggtcttttcg
     2521 attcatttga aaaaaatcgt caaaaagtat ttaataactt agctaatcta tttgaatttg
     2581 tgaaagagtt gggaagtttg tttggagatg ctatttatag ttggcaggaa tcggaagatt
     2641 ggacggaaca agaaaaattt tatatggaac aagagctttt agggataggt gtcagcaaac
     2701 atccactaca agctattgca agtaaggcta tttacccgat taccccaatc ggaaatttgt
     2761 cagaaaatag ctatgctatt atcttggttg aagttcagaa aataaaagtg attcgtacca
     2821 aaaagggtga aaatatggcc ttcttacagg cagatgatag taagaaaaaa ttggatgtca
     2881 ctctcttttc agacttatat cgtcaggttg gacaggaaat aaaagaggga gccttctact
     2941 atgtaaaagg aaaaatacaa tcacgtgatg gccgtctgca aatgattgca caagaaataa
     3001 gagaagcagt tgctgaacgc ttttggatac aggtgaaaaa tcatgaatcg gatcaagaaa
     3061 tttcacgcat tttagaacaa tttaaaggcc caatcccagt catcatccgg tatgaagagg
     3121 aacagaaaac catcgtttct ccccatcatt ttgtagctaa atccaatgaa ttagaggaga
     3181 aattgaatga aatcgttatg aaaacgattt atcgctaaaa atacggaaaa tagaagaatt
     3241 ttaaaatcaa atgtgatata atcaagaaga atgttaaaag aaaaaggagc ataaaccaat
     3301 atgaaacgta ttgctgtttt gactagtggc ggagacgcac ctggtatgaa cgctgccatc
     3361 cgtgcagttg ttcgtcaagc aatttcagaa ggaatggaag tgtttggtat ctatgacgga
     3421 tatgctggta tggttgccgg tgaaattcat cccctagatg cagcttcagt aggagacatc
     3481 atttctcgtg gtggtacttt tcttcactca gctcgttatc cagagtttgc tcaacttgaa
     3541 gggcaactta aagggattga gcaattgaaa aaacacggaa ttgaaggtgt agttgttatc
     3601 ggtggtgacg gatcttacca cggcgctatg cgtttgactg aacatggctt cccagctatt
     3661 ggtcttccag gtacaatcga taacgatatc gttggtactg actttacaat cggttttgac
     3721 acagcggtta ctactgccat ggacgctatc gataagattc gtgatacatc atcaagtcac
     3781 cgtcgtactt ttgtaatcga agttatggga cgtaacgctg gtgatatcgc tctttgggct
     3841 ggtattgcaa ctggtgctga tgaaatcatc atccctgaag caggcttcaa gatggaagat
     3901 atcgtagcaa gcatcaaagc tggttatgaa tgtggtaaaa aacacaatat catcgtctta
     3961 gctgaaggtg tgatgtcagc ggctgaattt ggtcaaaaac ttaaagaagc tggagataca
     4021 agcgaccttc gtgtaacaga acttggacat attcaacgtg gtggttctcc aactgcgcgt
     4081 gaccgtgttt tggcgtcacg tatgggtgca catgctgtta aacttcttaa agaaggtatc
     4141 ggtggtgttg cggttggtat tcgtaacgaa aaaatggttg aaaatccaat tcttggtact
     4201 gcagaagaag gggcattgtt tagccttact gcagaaggta agattgtggt taacaaccca
     4261 cacaaagccg atattgagct atctagcttg aataagagct tgtcataatt tacaatttag
     4321 ttaataagac caaaaaggtc atatatataa aggagtcaca aaaatcatga acaaacgtgt
     4381 aaaaatcgtt gcaactttgg gtcctgcggt agaaatccgt ggtggtaaaa aattcggtga
     4441 ggacggatac tggggtgaaa aacttgatgt tgaagcttca gctaaaaaca ttgctaaatt
     4501 gattgaagct ggtgctaaca cattccgatt caacttctca cacggcgacc accaagaaca
     4561 aggtgagcgt atggcaactg ttaaacttgc ggaaaaaatt gcaggtaaaa aagttggttt
     4621 ccttcttgat acaaaaggac ctgaaatccg tacagaattg ttcgaaggtg aagctaaaga
     4681 atattcatac aaaactggtg aaaaaattcg tgttgcaact aaacaaggaa tcaaatcaac
     4741 tcgtgaagtg attgcgttga acgttgctgg tgctcttgat atctatgatg atgttgaagt
     4801 tggtcgtcaa gttttggttg acgatggtaa acttggtctt cgtgtggttg ctaaagatga
     4861 tgcaactcgt gaatttgaag ttgaagttga aaacgatggt atcatcgcta aacaaaaagg
     4921 tgtgaacatc cctaacacta aaattccttt cccagctctt gctgaacgcg ataacgacga
     4981 tatccgtttc ggtcttgaac aaggtatcaa cttcatcgca atttcattcg tacgtactgc
     5041 aaaagatgtg aacgaagttc gtgcaatctg tgaagaaact ggaaacggac atgttcaatt
     5101 gttcgctaaa atcgaaaacc aacaaggtat cgataactta gacgaaatca tcgaagcagc
     5161 tgatggtatt atgattgctc gtggtgatat gggtatcgaa gtaccgttcg aaatggttcc
     5221 agtttatcaa aaaatgatta tcaagaaagt caatgctgca ggtaaagttg ttatcactgc
     5281 aacaaacatg cttgaaacaa tgactgaaaa accacgtgca actcgttcag aagtatcaga
     5341 cgtattcaac gctgttatcg acggaactga cgctacaatg ctgtcaggcg agtctgcaaa
     5401 cggtaaatac ccactcgagt cagtaactac aatggctaca atcgacaaga acgctcaagc
     5461 tcttcttaat gaatacggac gtcttgattc agattcattt gagcgtaact ctaagacaga
     5521 agtaatggct tctgctgtta aagatgctac taactcaatg gatatcaaat tggttgtaac
     5581 tcttactaag acaggtcata ctgcacgttt gatttctaaa taccgtccaa atgctgacat
     5641 cttagcattg acatttgacg aattgacaga acgtggcttg atgttgaact ggggtgttat
     5701 cccaatgttg acagatgctc catcttcaac tgacgatatg ttcgaaatcg ctgaacgtaa
     5761 agcggtagaa gcaggtctcg ttgagtcagg cgatgatatc gttatcgttg ctggtgtgcc
     5821 agtaggagaa gctgttcgca caaacacaat gcgtatccgc acagtacgtt aagaaaaata
     5881 taaaaaccta tcatatccag ctttagagct tgtgtgatag gctttttgta tagagggtaa
     5941 gaaagaggca aaactttcat aatggattga tactcttcga aaatcaaatt caaaccacgt
     6001 cagcgtcgcc ttaccttact caagtacagc ttgcggctag cttcctagtt tgctctttga
     6061 ttttcattga gtatgaaata agatatgcac aaattgatta gaaagtcaaa tgaatttcta
     6121 caaatgtttt agcaatcgta atgtacttgt ctagattcga tctgatatat tttcgattta
     6181 atgatatggt atttaaaacc tccaaagtag cttgctccat tcttttactt acgtgagtgt
     6241 agatgttatt tactgtttta gcgtttttgt gttccactct aaccattata gcattcttct
     6301 cagctagtgt actaaggagt gtgtgcctga aaatatggga actaaggggc tggtttatcg
     6361 gtttctctag tttagtattt gccttttgca aagtgatctt aaatgccttt ctctaaattt
     6421 acatatcact attgtttaac aaaatctaat ctactttagg tcacttattc tttttttgaa
     6481 atgtagaatg aactttttca aagtttttcg aatcttttaa aatctgtttg ttttatatcg
     6541 ccattctccc ccttttttaa ttctccctat atagcctgac aactttcccg atggtacgaa
     6601 tatggttgct ttcgtctagg tggatgtcgg ggtatttggg attgagtttt tttaaggtag
     6661 ccttggcgga gtttcttgac atagttagcg ccgtctactt ggaagatgcc gatggtatta
     6721 tagtcaatct gtggggtatt cttgataaat aggtagtcgc tgtttcttat ctttggctcc
     6781 atggacttgc tgacgacata agcgattggg tcgtagtcgt ctgggataat ggaaactcca
     6841 tatctaaatc gttgtcctgc atcgagcggc tacctgcaga gataaactac ctaacacgag
     6901 agtaagtagt ctgtctgtag tcgtccagtc tgatgatttt tacgatactt cgtttttctg
     6961 ctcatacagt tgcctctagg cataggttag gactttacct tgtctgggtg gtgcccgttg
     7021 gtcgtagata gattggatat cgctaggaga atccttttga actggaggaa agagggcatc
     7081 gatcaagcta ctgaatactt taactaagtc aaatatagta tttttcttag tagacctaac
     7141 ccttttttca taatttctaa tggtgttttt acttatacct atcttagtac ccaattctta
     7201 ttgagtccaa ccattactag tctatattgt tttatagttg attgagtttg gaatagtacg
     7261 ctgtagctgc taaaacattt ctaggaatta atttgccttt cctaatagag ttgttcatat
     7321 cttatttcaa tctactatat ttttcacctc taacaattgc aatctcttct ttatccatga
     7381 atgaaatcgc tttctgtttt tgtaagtaaa gcataacacg aaatccacga aaatgaaaac
     7441 ctttgttgtg ttttcgtaaa aaatttgttg acagagcacg aaacgcctac tataattaaa
     7501 tcaagcttaa ggaaataaca aaaaaacaaa tcggagggaa agacgatgaa cacattaaat
     7561 gagaaagtaa tcaatatctg taaagcagta gttaaagaaa ctttaatcca agacatttag
     7621 gagaaagaaa tgaagaaatc aagaaaatta gccacattag gaatctgttc agctttgttt
     7681 ttaggcttgg ctgcttgtca acaacaatat gctacttctg aggggacgaa tcaaaggcaa
     7741 agcagttcag cgaaagttcc atggaaagct tcatacacca acctaaacaa ccaggtaagt
     7801 acagaagagg tcaaatctct cttatcagct cacttggatc caaatagtgt tgatgcattt
     7861 tttaatctcg ttaatgacta taataccatt gtcggctcaa ctggcttatc aggagatttc
     7921 acttccttta ctcacaccga atacgatgtt gagaaaatca gtcatctctg gaatcaaaag
     7981 aagggcgatt tcgttgggac caactgccgt atcaatagtt attgtctttt gaaaaattca
     8041 gtcaccattc caaagcttga aaagaatgac cagttgcttt tcctagataa tgatgcaatt
     8101 gataaaggaa aggtctttga ttcacaagat aaggaagagt ttgatattct attttcgaga
     8161 gttccaactg aggcaactac agatgtcaag gttcacgctg aaaagatgga aacattcttc
     8221 tcacaatttc aattcaatga aaaagctcga atgctgtctg tagtcttgca cgacaatctg
     8281 gatggcgagt atctgtttgt aggccacgtt ggggtcttag tacctgctga tgacggtttc
     8341 ttatttgtag agaaattgac tttcgaagag ccctaccaag cgattaaatt tgctagtaag
     8401 gaagattgct acaagtattt gggcaccaag tatgcggatt atacaggcga gggactggct
     8461 aagcctttta tcatggataa tgataagtgg gttaaacttt aatagtgtta tgatatgaca
     8521 gtaatcttag gatttttatt tctatttttg atgagttggt gggttatttc aatcgttaat
     8581 agccagaaac cacgcaaaaa tgaaacattt attggctata tccaaagata cgatattgat
     8641 gggaatggct atcaatcatt accgtgtcct cagaactgag aggagttctt gaaaccgtaa
     8701 aaccgctttg aacaagagtt acttcaaccc attggctccg acggagtaag ttgctttcgt
     8761 gaataccaaa atcagccgca atttcttcat aagttcgata ttctcgcaca tattgaagag
     8821 tggccataag aaggtcttct aggcttaatt taggttttcg tccccctttt gcgtgtttaa
     8881 gttgataagc tgtttttaat acagctaaga tctcttcaaa agtggtacgc tgaacaccaa
     8941 caagacattt aaatcgtgca tcagttagtt gtttacttgc ttcataattc atagaactac
     9001 tataccatat tttgtttcgc aggaagtcta atgctaaaat cttatcagtg agaagatttc
     9061 ttaaagttta ttctttgtag tttcatctgt attagtacat aatttaacct agagaaaata
     9121 ggaggactaa tcgaatggaa ttaaaagatt ttacagaaaa agaacaggaa atgattaaga
     9181 aaaggcttac aatgtctaat attagtgaca aagaaactgc tgagaagatt ctggcgctag
     9241 taccacaaga cttgattaag cgaatcccgt tttttgtcag aaaacatgct acaacacgta
     9301 cgattaaacg catttcaatt gaacaccctg aactctacgc tgtagctcaa acaagtggtg
     9361 agattccaga aaaagaacgc gaagaattgc gtcagattat cacaactatc tttgaacaaa
     9421 agatgaataa gtatagtatt aagtagagaa tgaaaaaata ttttattggc ggtttgggaa
     9481 gcaatgccta tcatagcaag gattttcttc aagaactaga ttcgcaggtc tattttctaa
     9541 atccatatga aaagcatctt cgagatgaaa cagaattgaa atcatggttt aaaaatgaga
     9601 ttgtagagga ggaatctatc tgtctgatag gtcattctct tggaggagat ttagctcgtt
     9661 atttcgcatc ggaatttgaa gaagtaaaga aactgattct tttggatggt ggctatctag
     9721 atttagataa gattttacct ttggatacag agttagagga aactaaaaat tatatcaaat
     9781 ctcaaattat ttcggactta gctcttctta tttctaaaga aaaatctgaa gcaaagcatt
     9841 ggtcagaaaa tatggagaaa gccgtaagac agtcctatca ctggaatgtt gagtataata
     9901 gatatgagtt ggctataaat tatgaaaata tagaagcgat actccgccta cggagaaaaa
     9961 tacaagcttt taagagagaa gtgggagata ccttgtttat tagtcctcgc tatcctaatg
    10021 aagctacatg gagagaggaa gccctaaaag aattgccaga ctattttgat actatttttt
    10081 tagaaaactt tggccatgag ctttatactc aagtgcctaa agaaatcgct agtctaatga
    10141 atgagtggct cgcttatttt ctatgagcta cttgtattgc tggaaatggt ataagatgaa
    10201 aatctacaag tcactaattt tcatataaca aaaaacgaac aggctctttt tctgttattc
    10261 agaagagaac ttgttcgttt ttatcttgat tttcttaaaa agctctatat acatagggat
    10321 tttctggttt atcgactttg gtaaagctgt cgatttccaa ggttggaagg ttttgtttga
    10381 gttctttatg gtagtgattg accagttttc ctttggctga aatccaagtg ttgttttcat
    10441 actgccgggt attgcccttg gtcagcaatc catagacacc agaatctgcg atacagtgga
    10501 taatgccgaa gcggaacaga aattggctat tgtcatgact ggggtcgtta tagacaaagc
    10561 ctgtaaactg gattgtcttg ccctcaaact catctggata gtcgtagata gcctccatga
    10621 cttccatata gttttcatta gtgatctgaa tactatcttg ggataagtat ttatccgccg
    10681 ccgttcgcat ttccttttca taggctgatt ttgaaaaata agaactggta tctggtttca
    10741 aatattggct tgtcgtccct tcgcttgtct gaatggctag atccgttcct tctgataggg
    10801 ggaaatgata acctttagca gaaacggtct gagaatccaa gctaacagtt gggaaagtta
    10861 agccgatgac aagtggaata gccagaagag aaatactcgt catcttggcc aatcggctgt
    10921 tcagatgact gtgggttttg actcgcttca tccagatata caattgaacg atagccaaga
    10981 taaaagaaag taccatggaa atataggcta gataggaata gtgcatgttg atgtactggt
    11041 ttagtttgcc cgacagatgg aggtaaatag tcagttcaaa atagccagct aaaactaaaa
    11101 atcgaatcat aggatgactc caatcaagag agaatagact aagacgacaa gagttacaat
    11161 cgttatgaag tgactgataa atcgtgcttt caagtaattt ttcatcatga gaatattttt
    11221 gatatccagc attggaccaa tgacgagaaa ggccagaact ggtgccaaac cgaaactcga
    11281 gagaagagaa gcacctataa aggcatccgc ctcactacag agcgaaagaa gaaaggccaa
    11341 aatcatcaag agcaggatgg caaaaagagg ggttgcactg atagaggtca gaatccgagt
    11401 cggaacgtag acctgtatta tagaagcaaa gaggcaacca aataccaaat aacgccccgt
    11461 atcaaaaaat tcatcaatgg cctgcacaaa gacttgaaaa actttttttg cagaactcaa
    11521 gtaagaaaaa tcatgctcat gacaagccag acgattttct ttctgaatcg gttcttgcca
    11581 gaaaaatcct agaaaaattc ctagtattac agccacaaga atggaaccca gagctcgtaa
    11641 tagggcgaca tggaaggagt tgccaaaggc agaataggtc gcaaaaagaa caatgggatt
    11701 gataacaggt gctgtcacaa gaaaaggaac ggccgtgtaa cttggaacct ttttttccag
    11761 aaaacgattg ataatgggga cgattccaca ttcacaagaa gggaaaagca taccgacgaa
    11821 ggtcccaaaa aagattcttc cccaacgatt tcgagggaga aaatgataaa ccttttcagg
    11881 tgtgatataa acctcaatca gccctgagac aatgcttcct atcagaacga agggaagggc
    11941 ttcaatgata atggagagaa aaatggctcc agtttgaagt acactagatg ggagttgttg
    12001 aaaagcagtc atctagtttt tcttttctgc tttggctttt ttctctttgt catcagggaa
    12061 agtaacttgt ttcaaatcag ggagtttagc aaactcttca aacatcttgt ccaagtcgtc
    12121 tcgttttgta aattttacag ccataggtgt acctcgattc taaatcctac ctctattata
    12181 ctattatttt gcggaaaagg aagtttttta atttgttttc gatagttgat actcttcgaa
    12241 aatctcttca aaccacgtca gcttcgcctt tccgtagtac ggttactgac ttcgtcagtt
    12301 ctatccacaa cctcaaaaca gt
//
LOCUS       DAVKDZ010000058        12146 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000058 DAVKDZ010000000
VERSION     DAVKDZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12146
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.059"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(93..1484)
                     /locus_tag="VWZ30_001571"
     CDS             complement(93..1484)
                     /locus_tag="VWZ30_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU9335272.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIALPQALNGTFNPQAGTFVDLLPILVLA
                     LVTSLVLLNAKAALRFNSILVILKFSVLALFVLVGIWHIKLDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSQLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLMPAAFKELTKTSKVPKNATILTGLASAVAAGMFPLASIAAFLNTCTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMETWIAFLVALLIGSLI
                     YFTYGYKHSTIEE"
     gene            complement(1859..2425)
                     /gene="sdbB"
                     /locus_tag="VWZ30_001572"
     CDS             complement(1859..2425)
                     /gene="sdbB"
                     /locus_tag="VWZ30_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEU9335273.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEEAKKTQAPEQSPKQQQQTPVQ
                     QIAVGKEAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPD
                     RDFEILTVIAPGIQGEKTVEQFPQWFQDQGYKDIPVLYDTKATTFQAYQIRSIPTEYL
                     IDSQEKIGKIQFGVISNADAEAAFKEMN"
     gene            complement(2436..3143)
                     /gene="ccdA2"
                     /locus_tag="VWZ30_001573"
     CDS             complement(2436..3143)
                     /gene="ccdA2"
                     /locus_tag="VWZ30_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9335274.1"
                     /translation="METIVFSISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWLGLIRTLCFIAGISLIFFILGFGAGYFGHVLYANWFRYVMGVIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSVFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMAIPFLVLALASGIVMPYFSKLKRHMMLLKKIGGFLIILM
                     GILLLLGQVNVLAGIFE"
     gene            complement(3374..3790)
                     /locus_tag="VWZ30_001574"
     CDS             complement(3374..3790)
                     /locus_tag="VWZ30_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335275.1"
                     /translation="MKAALNSLKDVPANASDSKPAQDKKDAKQGTEDSKDSAKMTETT
                     SVPPGVIVVSLLVLLGVIAFWLVRRKKESEIQQLSTELTKILGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETEAKLVEELKSILAKLK"
     gene            complement(4077..4574)
                     /locus_tag="VWZ30_001575"
     CDS             complement(4077..4574)
                     /locus_tag="VWZ30_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEU9335276.1"
                     /translation="MKKIEVKGLAKTVKDKAIQNIENAQDETTLNTAKNNGITNIGKV
                     TLPRTTKKAEAKEAINAAAEAKKTEISNRFDLTIEDKSRALQQVDQEANDAKIAVDKG
                     VTNKDVEQAKVVGIDAINNINPQPTSRPLSTPQPQPSTPTQPDQGDTETPTTPSSPSA
                     QPSQL"
     gene            complement(4528..4701)
                     /locus_tag="VWZ30_001576"
     CDS             complement(4528..4701)
                     /locus_tag="VWZ30_001576"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335277.1"
                     /translation="MSDAVTIPTPTTPTLDKDGAKNEVEQAFSTKTSNIDKDNSLTNE
                     KNRSERISENSKR"
     gene            complement(4980..5703)
                     /locus_tag="VWZ30_001577"
                     /pseudo
     CDS             complement(4980..5703)
                     /locus_tag="VWZ30_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(6065..7828)
                     /locus_tag="VWZ30_001578"
     CDS             complement(6065..7828)
                     /locus_tag="VWZ30_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU9335278.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDTYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     SQEDPEYTDLLLKKALEFFEF"
     gene            complement(7837..8427)
                     /locus_tag="VWZ30_001579"
     CDS             complement(7837..8427)
                     /locus_tag="VWZ30_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU9335279.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTNVPLKSFINGLTGITAETLKDAPPVEKVIKEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            complement(8525..9016)
                     /gene="rocS"
                     /locus_tag="VWZ30_001580"
     CDS             complement(8525..9016)
                     /gene="rocS"
                     /locus_tag="VWZ30_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEU9335280.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            complement(9093..9785)
                     /locus_tag="VWZ30_001581"
     CDS             complement(9093..9785)
                     /locus_tag="VWZ30_001581"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU9335281.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIVTGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKTLD"
     gene            complement(9802..10116)
                     /locus_tag="VWZ30_001582"
     CDS             complement(9802..10116)
                     /locus_tag="VWZ30_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEU9335282.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            complement(10128..10673)
                     /locus_tag="VWZ30_001583"
     CDS             complement(10128..10673)
                     /locus_tag="VWZ30_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9335283.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            complement(10683..12062)
                     /gene="glmU"
                     /locus_tag="VWZ30_001584"
     CDS             complement(10683..12062)
                     /gene="glmU"
                     /locus_tag="VWZ30_001584"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU9335284.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVASISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
BASE COUNT         3646 a         2565 c         2115 g         3820 t
ORIGIN      
        1 atttaatctg tcaagatatt tttagaaaaa aattgaaatt tctttcacat ataaaaatct
       61 gctgagtttc aacaactcaa cagacttcca ctttattctt ctatggttga atgcttatag
      121 ccataagtaa agtaaataag acttcctatc aacaaagcaa cgaggaaagc aatccaggtt
      181 tccatattat actgcaacat gaaggataga caaatgatga ttgataaaat gggtaacaga
      241 ggtaccaatg gtgttttaaa ttccccagct ttgggcattc ctttttcttt ccgtaagcga
      301 atcagaccat aagccagcat gatcaagtag gctaaggtac aagtatttaa gaaggctgca
      361 atactagcta atggaaacat tcctgctgct actgctgaag ctagacctgt caagatagta
      421 gcattttttg gtaccttgct tgtttttgtt aattctttaa aggcagcagg cattaacccg
      481 tcacgcgcta aactgtaaat catacgcgat agtgcatagg tcatcgaaat acaaactgta
      541 atcaaggtca agatagccac taatgacaca tagttagctg cccaactaat cccaacgcta
      601 cgaagagcaa aggcaacggc atcatcgaca tttagttgac tatagtgaac cacaccagtc
      661 aagacaagcg tcaccaaggc atagagaatg gttacgatag aaagtgataa gacaatccct
      721 ctaggaatat tcttttgagg agtcttgact tcatctacag ccatagagat ggattcgaag
      781 cctaaaaatc caaagaacat caaggacgca ccagccataa taccagtact agcaccatag
      841 atttgtccaa aaccataagg agcaaaatta ctccaattat caagtttgat atgccaaatt
      901 cctactaaaa caaagagagc taaaaccgag aatttcagaa tgaccaaaat tgaattaaag
      961 cgtaaggctg ctttagcatt gagtaaaaca agcgatgtca ctaatgccaa gacaagaata
     1021 ggcaataaat caacaaatgt tcctgcctga ggattaaagg taccatttaa agcctgagga
     1081 agggctatcc cgtattgaga gagcaagcct ttaaaataag ctgcccaacc cgaagccaca
     1141 cctgatatgg ctgtcatgaa ctccatcatg gttaaccagc cagccaacca agctggaaat
     1201 tctcctaaaa tagcatagag ataactgtag gcaccacctg tagcgggtac tcgcgaggca
     1261 aattctgcaa aaaatagtgc tgataatccc acacataagg cagaaataac gattgaaatc
     1321 actagggctg gacctgctag agttgcggct gcagtacctg tgattgtaaa gacacctgtc
     1381 cctaccatgg ctccgatacc cagcaaaatc aaatcccata acttcaaatg cctgtgcatc
     1441 tcagttttat ccaaacttac attctttgtt ctaaatatat tcatctttga cctccaaaat
     1501 aaaatgatga ttctatttta ccataaatat aggagatatg tgaatattta taaaatgttt
     1561 ttctaaaaaa atctgactac cgtaacttct caaagtaact tccatctctc tctcaaaact
     1621 ggaagttact ctaagacgac tctgttttag ggctatttgt cgtgcacttt ttctgagtct
     1681 tttcgctttt gaacccttat atatcaagaa aaagaaaacc agtggaagtt agtctaagac
     1741 agatttccac tgatgtcacg cgtttttctc atactaaaag ctaggttgtg ggaaactgga
     1801 agttagtttt agaagttacc aaaatctggc tacatcttgt aatcagattt tatcgattct
     1861 agttcatttc tttaaatgct gcttctgcat ccgcgttgct gataacacca aattgaatct
     1921 ttccaatctt ttcttgacta tcaatcaggt attctgtagg aatgcttcga atttgataag
     1981 cttggaaggt tgttgctttg gtatcataaa gaactgggat atccttataa ccttgatctt
     2041 ggaaccattg tgggaattgt tcgacagttt tttcaccttg aattcctggt gcaatgacag
     2101 taagaatttc aaaatcgcga tctggttttg cagctaattc catcaactca ggcatacttt
     2161 tcttacatgg accacaccat gaagcccaaa acttcaagta gactttttta cccttaaaat
     2221 cagataactt cacttctttg ccatccatgg attgcaatgt gaagtctgga gcttcttttc
     2281 caacagcaat ttgttgtaca ggcgtttgtt gctgttgttt tggggattgt tctggtgctt
     2341 gagtcttttt agcctcttct tcaccacagg ccatcagtac aactaatgac aagaggctta
     2401 aaccagcaaa cattactttt ttcatttgtt ctcctttatt caaaaattcc tgctagaaca
     2461 ttgacttgtc ctaatagtaa caaaattccc attaaaataa tgaggaaacc accaattttc
     2521 ttcagtagca tcatatgacg cttgagttta ctaaaataag gcatcactat gcctgaagct
     2581 agagccaaga ccaagaaagg aatggccatg cccagagtgt aaataagagt atagatagct
     2641 ccttgccagg cgccattgcc tccagaagcc gcaagtgcta agacagaact taaaaccgga
     2701 ccaatacaag gcgtccaacc aaagctaaag gtaataccaa gtaaaaaaac tgaccaataa
     2761 cgattagaat ctgatttttt aaaggtaaaa cttttttgaa cttctaattt cttcaaatga
     2821 aaaatttcca tctggtgaag acccaaaatg ataataataa ctcccatgac atatcgaaac
     2881 caatttgcat agagaacatg accaaagtaa ccagcaccaa agcctagaat aaagaaaatg
     2941 agagagatac ctgcgataaa gcaaagtgtt cgaatcaagc ctaaccagag aacctttctc
     3001 ccaaacaaag aaaagctttt tgcactttct tgatcatcca ataaaatccc agcatagact
     3061 ggcagaagag gaaaaataca aggagaaaaa aaggataaaa cacctgctag aaaaacagag
     3121 attgaaaata ctatcgtttc caataaagaa ccaactttct taatcattct aatcctattt
     3181 tactatattc aattttattt gtaagctttc tgctacgcaa attcgtcttg aacactattg
     3241 gaccaatctt ttcttttgct agtcaaggcg gatcttatcc ccaaaaatag ccaaaaagca
     3301 acgacaagga ttactcgtcg ctgcttttgt gaacggaaat gtcttttagg tctgacgttt
     3361 cagaaatcac attttacttg agtttagcaa ggattgattt aagctcctct actagtttag
     3421 cttctgtctc tgctgagcca ttttcttctt ttacgaaatc aagggtttct tggagaaggt
     3481 tttgggcttt ggcaaggact tttttatccg ctttttctgc atctagttgt ccgagaatct
     3541 tggtcaattc cgtacttaat tgctggattt ctgactcttt cttacggcga accaaccaga
     3601 aggcaatcac gcctaggagg acaagtagac tgaccacaat cactcctggt ggaactgagg
     3661 ttgtttcagt catcttagct gaatccttac tatcttccgt tccttgtttg gcatccttct
     3721 tgtcctgtgc aggcttgctg tcgctagcat ttgctggcac atctttgaga gagttcaagg
     3781 cagccttcac agactctact gcagtatgca gaccttactc tgtcaaggca cgaagttcag
     3841 aacttgtcaa cttgctcttg agctggagct tgcgtgctcg ttgagctagc cgaagcgctt
     3901 gctaccactc taggatcttg agtcggagct gagcttggag ctgggacagg atttgcaggt
     3961 tgactaggag cagttatggt atattgaaac tagaatagta catattgact tctaaaacat
     4021 tgctaaaaat ttatttgatt atcctgatta ttttgtccta tttctgtttc attttattat
     4081 aattgagacg gttgagcact tggactagat ggagttgttg gtgtctctgt gtctccttgg
     4141 tctggctgag taggggtaga aggttgcggt tgaggcgtag aaagtggacg tgaagttggt
     4201 tgtggattga tgttattgat agcatcgatt cctaccactt ttgcctgttc tacatcctta
     4261 tttgttacgc ccttgtccac cgcaatctta gcatcgttcg cctcttgatc tacctgttgc
     4321 aatgctcttg acttatcctc aattgtcaaa tcaaaacgat tcgaaatttc tgttttctta
     4381 gcctctgctg ccgcattgat ggcttctttt gcttcagctt tcttagtagt tcttggtaac
     4441 gtgacttttc ctatgttagt aattccatta ttcttcgctg tgttcaacgt tgtttcatct
     4501 tgagcgtttt ctatattttg aatagcttta tcttttactg ttttcgctaa tcctttcact
     4561 tctatttttt tcattagtta aagaattatc cttatctata ttagaagttt tcgttgaaaa
     4621 tgcttgttct acttcatttt tcgccccatc tttatctaat gttggagttg ttggagttgg
     4681 tattgttacc gcatcactca tgtctgatgt agatctttct acattaatat ttccaccata
     4741 agtagctggt atatattctc tgtctctagc tatgtttcta gtagttattt tatctccagc
     4801 ttgtaatgct tcatccggtg tccaactagc agttccgttt acagcagtaa ttgttgcttt
     4861 ttagtgttat ttaaataaaa ttcaacaata gaatcattag aatttctcgc tgttgattta
     4921 actatcactc gtttacctgc aacattttcc aaattttcta ccgtagactt cctgcaaaac
     4981 tagaatccta gttcatgatt ggtaatacca gcaatcaaat tcattcgtaa tccgaagcgt
     5041 ttacgatgat ttcgataggt tgttgaaaac attttaaacg tttttacttt ggcaaagatg
     5101 ttttcaacct tgcttctctc cttagatagc gcatggttac aggctttatc ttcaactgtt
     5161 agcggcttga gtttgctgga tttacgtgga gtttgtgctt gaggatatat cttcatgagc
     5221 ccttgataac cactgtcagc caagatttta ccagcttgtc cgatatttct gcgactcatt
     5281 ttgaacaact tcatatcatg acaatagttc acagtgatat ccaaagaaac aattcttcct
     5341 tgacttgtga caatcgcttg agccttcata gcgtggcatt tctttttacc agaatcattc
     5401 gctaattctt tttttagggc gattgatttg tacttccgtc gcatcaatca ttaccgtgtc
     5461 ctcagaactg agaggagttc ttgaaatcgt aaaaccattt tgaacaagag ttacttcacc
     5521 ccattggctc cgacggatta agttgctttc gtggatacca aaatcagccg caatttcttc
     5581 ataagtgcgg tattctcgca catattgaag agtggccata agaaggtctt ctaggcttaa
     5641 tttaggtttt cgtccacctt ttgcgtgttt aagttgataa gctgttttta atccagctaa
     5701 catctcttca aaagtggtac gctgaacacc aacaagacgc ttaaatcatg catcagttag
     5761 ttgtttactt gcttcatcat tcatagaact actataccat attttgtttc gcaggaagta
     5821 taatttaatt tactagattt gatgcttcta tctatttatt cggaaaagaa attaaaaatc
     5881 ttcccaattt ctagtaatat tttagaagtt atagtgtagc attccaactt caaagcacta
     5941 taaagtaaat tgaaacaaga acaatacaaa caattctcgt aaacggattg caaccacaaa
     6001 aaagcaagca ttcacaagaa tacttaccta tcatgggagg aacaaccgtt cctctttttt
     6061 attactaaaa ttcaaagaat tccaatgctt ttttcaagag caaatccgta tattctggat
     6121 cttcttggga tacttctatt tcccgctgaa ctttttccaa atcatctgta atcactccat
     6181 ctactcctaa gtgaagagat ttgctgatag cttctgaatc attgacagtc cagacataaa
     6241 gtttctgatc cgttgtccat agtttgctta caaaatattc atccaaggtt gagtactcca
     6301 tagtatatcc tgtcgctctt gttttaggaa agacagaatt gtagggcatg atgaaataaa
     6361 ctggcagttc ggcatcatac tgtcttactt tttcgacaac atggtagtct aaagactgga
     6421 tttgatgtcc ataaatcttg agctttgcag cataacgggc taaaaagcgg ttcatcatgt
     6481 ctggactatc ttttttactg gttttaattt caattagtaa tttttgacca agttcgttgg
     6541 ctcgactgag ataatcttca aagcttgaaa ttttagtctg gtagccattt tcaaaaatat
     6601 caatcccttt aagctcctcc aagtttaagt cttgaggact tttattgata cctgctagat
     6661 ttttcaagtt agcatcatgc atcatgacaa actgcccatc ttttgtttcc tgcacgtccg
     6721 tctccaccaa gtctggtttg agttgtgctg tagtttccaa ggactctact gtattttgaa
     6781 tcccatttgc attggaaacc cctcggtgag aaataagttg aggtagatga accatgggag
     6841 cctccagata aatataacct tctaaggcaa agaaaagact ggcacaagtc atgacacccc
     6901 atcgcacgat gtgatctttt tctctcctag gaagcatatc cagctccttt cctgtcaaaa
     6961 atgaaacaaa tttaaccaaa aaataagtca gagccatata atagagattt ttaatcacga
     7021 caaaattcaa aataccaaga atcagagact ctctctgagt gatatcatct accaaagttt
     7081 gagccaataa taaaggaatc aaaggaagat aaaataataa atgtgctttg agcaagatgt
     7141 aaaataaatt ccaagcataa aaagtaactc tcttcttggt tttctccaag ctaaacatca
     7201 ctgcttctcg aacagtcagc tgatcatata caatcttcgg aagggcaaac atcaatctga
     7261 cagagacata gagaaagata agagatagaa gtaggatgct cagccaccac atccaatatg
     7321 tatcttctaa ataagcttgg ataaactctg gaatgacgat tttattaaga taataaatct
     7381 tcagcatttt ccgtataaaa ggaaaaagca tagctatata gaaaaagata aacaaggctt
     7441 tagcgcaagt tagctttttc ataaatccaa aactttcatg gaaaaccttg cggatatact
     7501 caattagcct tcgcttttca ttatagagga gatgacgagc gccaataaag aggagtccta
     7561 tttgaaaata agcaaccaga aggttaatta caatcaaggc taaaaaagct agactaatca
     7621 atggagaatg agtaaggatg gctaagacat tgttatagga aataaaaaga taacctgtct
     7681 gatctaataa gaagctagcc aaccatgaat tgaatggtac ccacaaatac tccactatca
     7741 taaaaatcaa gaaaaataga aagaggattt tatcaagatt gaggtaaatc tgtttaagac
     7801 ccaatttttt aggtttttca ggtttcatag gcactcctag tcaaataatt gagacaagtc
     7861 caagccacca aaaggattgt ttgataagct actttctgtc tctaacaatt ccctagcttg
     7921 atccgactct aagaaggatt cgtaaacacg cgccgtcatc cgagcatcct ctaaactatt
     7981 atgagactga ccttgaaatc caagaaatga ggcaacagtt tgcaatttga gattggcaat
     8041 accatgtaaa tctgaactcc gacgttcaaa agcttcatca tacaaatcca ccttgtactg
     8101 ttggctatag tctaaaccat gctctgctaa aataggtaaa tcacttttag cagcattgta
     8161 gcctatcatc agcaaagaac ccacaaactc ttggaattct tttataactt tctccactgg
     8221 tggagcatct tttaaggtct cagccgtaat ccctgtcaaa ccattgataa aactttttag
     8281 aggtacattg gtatgaacat aggaatcata ggcatcgatt tcccgaccat ctctaaaacg
     8341 cactgccgat acttgaatca agtgtgtttg tccttcgtgc tgattaaatt ctaagtcaaa
     8401 agcaatgtaa tcttctaatc gttccattct gtacctctcc tctactgtct ctgttctaat
     8461 caactatact attcacaaac aaaagaagcc atcaggttag catttaacct aaacagctcc
     8521 ttgattatcc tccaaataaa cgagcaaaaa agcctttctt agtggattgg acttcttctt
     8581 ttgcttggtc cagctctagc ttaagatttt cttgatcctt catggcttga agggtcaatt
     8641 gttgttgctg atctaattgt ttgtctttct cagcaatctg acggtctttg atacgcatct
     8701 gctcgtcttt ttctgataac tgacgatcct tggcttttaa ttgttcataa agacgaagaa
     8761 tttctgcatt cttctcatcc actagaatct ccatcagttc acgttgtttg acatcttcac
     8821 tgacaggttc atcttcaaaa atcgtttttt tatagatttc ttctagctta atcaagccac
     8881 ttctggtaac aactgttacc cctttgtcat ttttatctgt gtcttcttct ggtaattctt
     8941 tgacacggtt attgattgct tggcgagata atcctaagac ctctgcaatc tcactgacgg
     9001 tcatttcaat actcataata tcctctgaaa cgttttctag cttttcttta cttaaatctt
     9061 atcataagct agaaaaactg tcaaatttcc gcttaatcta aagtcttcaa aaaggtcaac
     9121 aagacttggg cagagcgacg tccagcttcg ataataaact catcaaaaaa gatgtttgct
     9181 tcatggttgg cattgtcact catagctctg atgactaaga ctgggagatt gagggcatgc
     9241 gctgcttgag caatagctgc cccctccatc tccacggcta aaacttctgg gaaatgggac
     9301 ttaatcgctt ctatcttgtc atttcctgca acaaaactat ctcctgtaac aatcaaacca
     9361 agatgccagt tttggtccaa ttgagataaa ctctcttgga tttgagcaac aaaggttttg
     9421 tctgattcga aataaagcgg ttgttgcgcc atttgcccat aagcatagcc aaaagctgtg
     9481 acatccacgt catgataggc taatttgtca gcaatcacga catccccaac agcgatacct
     9541 tctgctactg ccccagctga acccgtatta ataagggcat ccacctggaa atgatcagcc
     9601 aaaatcgcca cactcatagc agacatgacc ttaccaattc cactttctac aagaacgact
     9661 tcatgagaag caatggttcc tgtatgatag gtattcccca aaacaacttg ctcctgggta
     9721 ttatctaaat gctggaccag ataagccagt tcttctggca tagcagcaat aattcctatt
     9781 ttcatttcaa ttccttttct attacaaaag tttcattgct aaaacaagca aaatcaagag
     9841 aaagatgacc gcaaataaga ttttattcaa cttagaattg aagacatttc tcttggtatt
     9901 ttcaatacga cggcttttat gaatagatgg ttcgacctga atcttcaagg tttcctggct
     9961 aaaaccatga tccttaggat tggcataacc aaaacgggaa gaagaggttg gtaaaatctt
    10021 agtttcctca ttatcatcta gcaaaggagg acctgaaatt ttttcgcctc tattagctct
    10081 ttcaatcatt tcatccgtta ataaagattt acccatactg acctcctcta ttttttctgc
    10141 aactcccaat actgaacagc cataattgtc ttggcatcac agatatgacc tgattggatt
    10201 aattctttcg cttcttctaa gctcacttca aggacttcca aggtttcatc ctcatcctgc
    10261 ggacgcggat tttccacttt tgtcaaatcg cttgctaaat atagttttaa cttctcatta
    10321 caaaagccaa tagctgaata aaaatcgtac aagagttcta atttccctgt ataggctgtt
    10381 tcttcctcta attcacgaag ggcagctgcc acaggggctg tgttttctcc tacctccaat
    10441 tttccggctg gaatttcgta agagacagcc tcgatagctt tgcggtactg cttgaccaag
    10501 ataagttttt gttcatccgt tactgctaaa acacagacag ccccattgtg gaaaatcaaa
    10561 tcccgttggg cagttccctt gccttctggt aattcaacct gatcttggac cagtttaaat
    10621 attggtcctt gatagatttc ttttcggcta agcgtttttt cttcaaactc catgataggt
    10681 tcctactggt tcttaggatg atgaggaaga cgtgttgcat attcgtcttt attgatctga
    10741 cgaccgcgac caatagcaat agcatctgct ggcacgtctt tagtaatagt tgaaccagca
    10801 ccaacgaggg aattgtcacc aagttctact ggtgcaataa tggttgaatt tgaaccaaca
    10861 aagacattgt ctccaatgac tgtcttgtat ttgtttttgc cgtcatagtt gactgtaata
    10921 gttccagcac cgaaattaac gttgcttccc acttcacagt ttccgatata agtcaaatga
    10981 ccagccttgg tattctcacc gattgaagat cctttcacct caacaaagtt accaatatga
    11041 acttgggcac ccagacttga atttggacga atgtgagcat aaggaccgac tgtcacaccg
    11101 tctgcaacac tactttcctc aatcatagaa ttggtaatga ccgctcctgc tccgatagtg
    11161 ctgtccacta cataagtacc gtttgtcaaa acagtctcag caccaatttt cgtttgccct
    11221 ttcaaggtaa cattggcttc gatttgaact tccggagcaa tctcaacatc aatatcgata
    11281 taagttgctt ctggattgac aaagctaaca ccgttgacca tgtgtttatg attgatgcga
    11341 cgacgcataa ctgactcagc tgtcgcaagc gccacacggt catttacccc aagactttca
    11401 tcaaaatctt tcaaagtata agcgccaact ttttcaccag tttcacggaa aataccaatg
    11461 acgtctgtaa tatagtattc gccttgagcg ttattggtat tgatattttt caaagcctca
    11521 aacaaacgct cgttgtcaaa gacgtatgtt ccagtgttga tttccttgat ttgcttttca
    11581 aaatctgtag catccttctg ctcaacaata cgaagaacct cagcattgtc attacgaaca
    11641 attcgtccat agccaaaagg attatccgtt tcagcagtca agatagtggc cacattttta
    11701 tgattgatat ggaaatcaat caagtttttc aagctttcac cagtgattaa aggagtatct
    11761 cctgcaatga ccaaggtgtg tcctgacaaa ccttctaaga taggttctgt catcataact
    11821 gcatgaccag ttcccagctg ttcagattga gtcacaaatt ctgtctgtcc agccaagacc
    11881 tcctcaacca attctgcctt gtgtcctaca actgttactg tcttttcagg ttggatagct
    11941 cccacactac ggaaaacatg ttccaacata gaaatactcg caaccttgtg caaaactttt
    12001 ggcaaatcag atttcatgcg agtcccttta cccgctgcta aaataatggc aaaatttgac
    12061 ataattttct cttttctgta gaaattccct tttattatac catatttcaa atcattccgc
    12121 actcttgaat gaaaaaatgc tctaaa
//
LOCUS       DAVKDZ010000059        12137 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000059 DAVKDZ010000000
VERSION     DAVKDZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12137
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.060"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            167..1192
                     /gene="trpS"
                     /locus_tag="VWZ30_001585"
     CDS             167..1192
                     /gene="trpS"
                     /locus_tag="VWZ30_001585"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU9335285.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQNIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            1344..2822
                     /gene="guaB"
                     /locus_tag="VWZ30_001586"
     CDS             1344..2822
                     /gene="guaB"
                     /locus_tag="VWZ30_001586"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU9335286.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(2873..3970)
                     /gene="recF"
                     /locus_tag="VWZ30_001587"
     CDS             complement(2873..3970)
                     /gene="recF"
                     /locus_tag="VWZ30_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU9335287.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGYRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQNGKAAVNGN"
     gene            complement(3973..4341)
                     /gene="yaaA"
                     /locus_tag="VWZ30_001588"
     CDS             complement(3973..4341)
                     /gene="yaaA"
                     /locus_tag="VWZ30_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEU9335288.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            4500..5750
                     /locus_tag="VWZ30_001589"
     CDS             4500..5750
                     /locus_tag="VWZ30_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9335289.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSQLDLFSGFLRMYAGIDRENRNQARKMMNNQLLDL
                     KKGYFTELELEQTKEMIRRSLLLSQDNQGSLIERAYQNALLGKSSADFKSWITKLEQV
                     DKDDICRAANNVKLQAIYFMEGIE"
     gene            5747..7030
                     /locus_tag="VWZ30_001590"
     CDS             5747..7030
                     /locus_tag="VWZ30_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9335290.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTLVTEVDGDVKEYPAGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTSYLFSATDHFLENLDLLDELVTSAHFTEASILREQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTRFYKPVNMSLFLVGDFDVELV
                     QGYFERKELKDLDVQEVVREKFVLQAVKQTDSMRMEVSSPKLAIGVRGKREVAEADCY
                     RHHVLLKLLFAMMFGWTSDRFQKLYESGKIDASLSLEIEVTSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            7072..7893
                     /locus_tag="VWZ30_001591"
     CDS             7072..7893
                     /locus_tag="VWZ30_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9335291.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKGPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            7904..8449
                     /gene="pgsA"
                     /locus_tag="VWZ30_001592"
     CDS             7904..8449
                     /gene="pgsA"
                     /locus_tag="VWZ30_001592"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU9335292.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            8446..9273
                     /locus_tag="VWZ30_001593"
     CDS             8446..9273
                     /locus_tag="VWZ30_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9335293.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALDLVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            9258..10097
                     /locus_tag="VWZ30_001594"
     CDS             9258..10097
                     /locus_tag="VWZ30_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9335294.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            10090..10884
                     /locus_tag="VWZ30_001595"
     CDS             10090..10884
                     /locus_tag="VWZ30_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9335295.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            10943..11761
                     /gene="mreC"
                     /locus_tag="VWZ30_001596"
     CDS             10943..11761
                     /gene="mreC"
                     /locus_tag="VWZ30_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU9335296.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSISKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            11761..>12137
                     /gene="mreD"
                     /locus_tag="VWZ30_001597"
     CDS             11761..>12137
                     /gene="mreD"
                     /locus_tag="VWZ30_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU9335297.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMV"
BASE COUNT         3694 a         1823 c         2602 g         4018 t
ORIGIN      
        1 aaaatacaga aatattcaaa tttttatttg tcaattttgt gtaaattata tttacagtat
       61 cctttacaca aatctgtaaa aagcaaggct gatttatttt gataaattac ggttatttca
      121 ttaaaaaaat gctataattg aaaggactat atcgaaggag aacaaaatga ctaaacccat
      181 tattttaaca ggagaccgtc caacaggaaa attgcatatt ggacattatg ttggaagtct
      241 caaaaatcga gtattattac aggaagagga taagtatgat atgtttgtgt tcttggctga
      301 ccaacaagct ttgacagatc atgccaaaga tcctcaaaac attgtagagt ctatcggaaa
      361 tgtggctttg gattatcttg cagttggatt ggatccaaat aagtcaacta tttttattca
      421 aagccagatt ccagagttgg ctgagttgtc tatgtattat atgaatctag tttcgttagc
      481 acgtttggag cgaaatccaa cagtcaagac agagatttct cagaaaggat ttggagaaag
      541 cattccgaca ggattcttgg tctatccaat cgctcaagca gctgatatca cagctttcaa
      601 ggctaattat gttcctgttg ggacagatca gaaaccaatg attgagcaaa ctcgtgaaat
      661 tgttcgttct tttaacaatg catataactg tgatgtcttg gtagagccgg aaggtattta
      721 tccagaaaat gagagagcag ggcgtttgcc tggtttagat ggaaatgcta aaatgtctaa
      781 atcactaaat aatggtattt atttagctga tgatgcggat actttgcgta aaaaagtaat
      841 gagtatgtat acagatccag atcatatccg cgttgaggat ccaggtaaga ttgagggaaa
      901 tatggttttc cattatctag atgtttttgg tcgtccagaa gatgctcaag aaattgctga
      961 tatgaaagaa cgttatcaac gaggtggtct tggtgatgtg aagaccaagc gttatctact
     1021 tgaaatatta gaacgtgaac tgggtcctat tcgtgagcgc cgtattgaat ttgctaagga
     1081 tatgggagaa gtttataata tgattcaaaa aggtagtgaa agagcgcgtg aagttgcggg
     1141 tcaaacccta tctgaggtaa aaggggcaat gggacttcat tactttaact aagtttattt
     1201 tacaagaaac tatcatttct atctcgaaga aaagatagtt ttttatttac ccctgtaaca
     1261 tttgacaaat ttttatagaa tggtatgata gatacaatat aaaaagaatc aagctcaaaa
     1321 agaaagaaaa gaggaaactt cgaatgtcta attgggacac taaatttttg aaaaaaggtt
     1381 ttacctttga tgatgtattg cttattccag ctgaaagtca tgtgttgcct aacgatgcag
     1441 atttaacaac taaattggca gataatttga ctttaaatat cccaattatt accgctgcca
     1501 tggacacagt tacagagagt caaatggcca ttgctattgc tcgtgcaggt ggtctcggag
     1561 ttatccataa aaacatgtca attgctcaac aagcagacga ggttcgtaag gtaaaacgtt
     1621 ctgaaaatgg agttattatt gatccgttct tcttgacgcc tgaacataca attgctgaag
     1681 cagatgagct tatgggtcgt taccgcatca gtggtgttcc agttgttgaa acacttgaaa
     1741 atcgtaaatt ggttggtatt ttgacaaacc gagatcttcg ttttatttca gattataatc
     1801 aaccaatttc aaaccatatg actagtgaaa atcttgttac tgctcctgtg ggtacggatc
     1861 ttgcaacggc tgagagtatt cttcaagagc atcgtattga aaaacttccg ttggtcgatg
     1921 aagaaggcag tctttctggt ttgatcacta tcaaagatat tgaaaaagtt attgagtttc
     1981 caaatgcggc taaagatgag tttggtcgtc ttctagttgc aggtgcagta ggtgttactt
     2041 cagatacatt tgaacgtgca gaggctcttt ttgaggcagg agcggatgcg attgttattg
     2101 atactgcaca tggtcattct gcaggtgtct tgcgtaaaat tgccgagatt cgtgctcatt
     2161 tcccagatcg gactttgatt gctggaaata ttgctactgc tgaaggtgca cgtgcccttt
     2221 atgaagcggg tgtagacgtt gttaaggttg gtattggacc aggttctatc tgtactactc
     2281 gtgtgattgc tggtgttggt gttccgcaag taacagctat ctacgatgct gcagctgttg
     2341 cgcgcgaata tggtaaaacg attattgctg acggtgggat caagtattct ggagatattg
     2401 taaaagcact tgctgcaggt ggaaatgctg ttatgcttgg atctatgttt gctggaactg
     2461 atgaagctcc aggcgaaact gaaatcttcc aaggacgtaa attcaagact taccgtggta
     2521 tgggatcaat tgctgctatg aagaaaggtt caagcgaccg ttatttccaa ggttctgtca
     2581 atgaagcaaa caagctcgtt ccagaaggaa ttgaaggtcg tgttgcttat aaaggagcgg
     2641 cagctgatat tgttttccaa atgattggtg gtattcgctc tggtatgggt tactgtggtg
     2701 cagctaacct taaagaacta cacgataatg ctcaatttat tgaaatgtct ggtgctggtt
     2761 tgaaagaaag ccatcctcat gatgtgcaaa ttactaatga ggcaccaaat tattctatgt
     2821 aaaaaacaat gaaaagaact ccagtgaaaa caggagttct tttacaatgt tgtcaatttc
     2881 catttacagc agctttacca ttctgaatag tgaagatact tagattttct ggcagatttt
     2941 gaagatggtc taagcttgtt gttgtgataa aggtttggat tgattgagaa atcgtttcta
     3001 ataattttaa ctgtctagtg ttgtcaagtt cactcatcac atcgtcaagc agtaatatcg
     3061 gagattctgt ggtaatgctt tccattaatt cgatttctgc taattttata gagaggacga
     3121 gactacgatg ttgaccttgg cttccgaaac tagcatccat cccatttata taaaaagaaa
     3181 tgtcatctcg atgaggaccg acaccagtat tctttttaaa taaatctctg gatctacttt
     3241 tttctaaagc aattttgaaa gattcggata agttttgttt gtcagttata ttgacagaag
     3301 attgatagga tattgacaac tcttcgatct gattagagag ttcaaaatgt ttcttacgcc
     3361 caaatgattc tagttttttt atgaaatcta agcggtgatt cattacacga tatccataat
     3421 caactagctg atcatctaac acagaaagga atgtttcatc tattttttga gctgatttta
     3481 ggtaagtgtt tctttgcttt aggatgtggt tataattggt taagtcagat aagtagattg
     3541 gcttaatttg cccaagttcc atatcaatga attttcgtcg aatcgaaggt gctcctttaa
     3601 ttagttgtaa atcttcagga gcaaataaga caacattcat gtgtcctaca taatctgaaa
     3661 ggcgtgcctg ttttaagtga ttaacttttg ttacacggcc tttttgtgtt agttcgattt
     3721 ctaggggaat ggatccagtt tttttctgaa cgagacctga aagatgaagt tgttcctcat
     3781 caaaatgaat gagattttta tctgttcgag ttcgatgact acgcgttaag gctaaaaaat
     3841 agatagcctc taacatgttt gtttttcctt gtgcattacg tcccaaaaag acattcaatt
     3901 taggattaaa gtctattttc gtttctttgt aattacgaaa agtcttgaga gatagatgtt
     3961 gtagccacat gattatctac cagggaatcg tggagcttgt tggcttttag gtgatgaagc
     4021 aggtttcgat ttgtcttttt taactccctt attcatcttt ttgacaagtt tagcgatccg
     4081 ttctttttca actttatcag cttggtattc atcttgttct tcagaagtag gttgtgtcaa
     4141 caagatgtca atgttcatgt cagggatgtc aactttatcg ccaatacgaa gtttttttcc
     4201 acgacgactc tctaattccc tattaaagta aacagaatgt tcagagagaa atgatttaat
     4261 agctcctccg ctatgtgtaa ttccaagttc tttgagtagt gcttggaggg taataaattc
     4321 ttcaaataat ttgtattcca taattcacct caatctttgg tattatacca tattttccta
     4381 aaaatagtga gagagacagc gagaaatgta agcgattttt atttcaaaag tgttacaggg
     4441 aacaagaaaa tttcaggttt tcgtgatata atagaagtct gtatataagg aggtaaatca
     4501 tggagttagt gcatggaatt tcaacacatt ttatccaatc aaaaaagttt aaaacaaaca
     4561 aaattaccgt gcgttttacc gctccattat cccttgatac gattgcaggt cacatgttga
     4621 gtgcaagtat gctagagact gctaatcaga tgtaccccac ttctcaagat ttgaggagac
     4681 acttggccag tctatacggt acagatatgt caaccaattg tttcagaaga gggcaaagcc
     4741 acattataga attgacattt acctatgttc gtgatgagtt tttaagtagg aaaaacgtgc
     4801 taacttctca gattttggaa cttgtaaaag aaactctttt ttcacccgta gtagttgata
     4861 atgggtttga tccggcctta tttgaaattg agaaaaaaca attgctagca agtttagcag
     4921 ctgatatgga tgattctttt tattttgcac ataaagaatt ggataaattg ttttttcatg
     4981 atgaacgtct tcaattggaa tatagtgatt tacgaaatcg tattttagct gaaactccac
     5041 aaagttctta ttcttgtttc caagaatttt tagccaatga tcgaatagat ttctttttcc
     5101 taggtgattt taatgaggtt gaaattcaaa atgtattaga atcatttggc tttaaaggtc
     5161 gaaaaggaga tgtgaaggtt cagtattgtc aaccttattc taatatcctt caggaaggta
     5221 tggttcggaa aaatgtggga caatctattt tggaattagg ttatcattac cgttctaaat
     5281 atggtgatga gcaacattta cccatgattg taatgaatgg tttacttggt ggatttgctc
     5341 actctaagct ctttacaaat gtccgtgaaa atgctggatt ggcttatact atttcaagtc
     5401 agcttgattt atttagtgga tttttgagga tgtatgctgg tattgatcga gaaaatcgga
     5461 atcaggctcg taaaatgatg aataatcaac tgcttgattt aaaaaaaggt tattttacag
     5521 agcttgagtt agagcagacc aaggaaatga ttcgtcggtc tttgttactt tctcaagata
     5581 atcaaggttc attgattgaa cgtgcttatc aaaatgcctt acttggaaaa tcttcagcag
     5641 actttaaaag ttggattaca aaactcgagc aagttgataa agatgatatt tgtagagcag
     5701 ctaataatgt gaaactacaa gcgatttact ttatggaagg aatagaatga caaaggttgt
     5761 ttttgaagaa aaatactatc cagctgtaaa agaaatggtt tatcgaactc gtttggccaa
     5821 cggattgaca gttgctcttt tgcctaaaaa ggaatttaaa gaggtttacg ggagtgtaac
     5881 tgtacaattt ggttcggtag atacgcttgt cacagaagtt gacggagatg taaaagaata
     5941 tcctgcggga attgctcatt ttcttgaaca taaattattt gagagagaag attctagtga
     6001 tttgatgtct gcttttacga gtctaggtgc agatagtaat gcctttacaa gctttacaaa
     6061 aacaagttat cttttttcag caacggatca ttttttagaa aatttagatt tacttgatga
     6121 attggtaaca tcagcacact ttactgaagc ttccattttg agagagcagg atattatcca
     6181 gcaagaacga gaaatgtatc aagatgatcc agattcgtgt ttattctttt caactttagc
     6241 gaatttgtat cctggtacac ctttagcaac ggatatagtt ggaagtgagg agtccatttc
     6301 ccaaatcaat ctaactaatt tgcaagaaaa ttttacaaga ttttacaaac ctgtaaacat
     6361 gtctctgttt ttagttggtg attttgatgt agagctagtt cagggttact ttgaaagaaa
     6421 agaactgaaa gacttagatg ttcaggaagt agtaagagaa aaatttgttt tacaggctgt
     6481 aaagcaaaca gacagtatga gaatggaagt atcttctccc aaactagcga ttggagttag
     6541 aggtaagcga gaagttgctg aggcggattg ctatcgacat catgttttat taaaattatt
     6601 gtttgcaatg atgtttggtt ggacttcgga tcgttttcaa aagttatatg aatcaggtaa
     6661 aatagatgct tccttatctc ttgaaattga agtaacaagt cgttttcatt ttgtcatgtt
     6721 gacaatggat acgaaagagc cagttgcttt gtctcatcaa tttaggaagg ctattcgtaa
     6781 ttttacaaag gatttagata ttacagagga acatttagat attatcaaaa gagagatgtt
     6841 tggcgaattt ttcagtagca tgaactctct tgaatttatt gcaacgcaat atgatgcttt
     6901 tgaaaatggt gagacaattt ttgatttgcc gaaaatttta caggaaatta ctttagagga
     6961 tgtccttgat gctggacatc atttaataga tgatggtgac atagttgatt ttacaatatt
     7021 cccatcgtag taacctatta taatagacac tagaaagaag ggatgacaag tatgagaaaa
     7081 aaaacaattg gagaggtttt acgattagct agaatcaatc agggattgag tttagatgaa
     7141 ttgcagaaaa agacagaaat ccagttagat atgttggaag caatggaagc agacgatttc
     7201 gatcaacttc caagtccttt ttacacgcgt tctttcttga aaaaatatgc atgggctgtt
     7261 gagttagatg accaaattgt tttggatgct tatgattctg ggagtatgat tacttatgag
     7321 gaagtagatg ttgatgaaga tgagttgaca ggtcgtagac gttcaagtaa gaaaaagaag
     7381 aaaaaaacat catttttacc tttattttat tttatccttt ttgctttatc gattttaatt
     7441 tttgtgactt attatgtttg gaactatatt caaactcaac cagaggagcc ttctctttct
     7501 aattacagtg tggttcaatc aacaagttca actagctctg ttccccactc ctcaagtagt
     7561 agttcttcta gtatagaatc agctataagt gtatcaggcg aaggaaatca tgtagaaatc
     7621 gcttataaga caagtaagga aacagttaaa ttgcaattgg cagtttcaga tgttacaagt
     7681 tgggtcagtg tttcagaaag cgaacttgag ggcggtgtaa ccttatcgcc aaagaagaaa
     7741 agtgcagaag caacagttgc aactaaaggt cctgtaacaa ttacgttagg tgttgtaaaa
     7801 ggtgttgatt tgacagtaga taatcagact gttgatttat cgaaattaac agctcagact
     7861 ggacaaatca ctgtaacctt tactaaaaat taaggaaaaa cgaatgaaaa aagaacaaat
     7921 tcccaatctc ttaacaatag gtcgaattct ctttatacct atttttatct ttattttaac
     7981 gataggaaat tcgatagaga gtcatatagt tgcagctatt atctttgctg ttgccagtat
     8041 taccgactat ttagatggat atttagctcg taaatggaat gtggtcagta attttggtaa
     8101 atttgcagat cctatggcgg ataagttact agttatgtcg gcttttatta tgttgattga
     8161 gttaggtatg gctccggctt ggattgttgc agtgattatc tgtcgtgagt tagctgtgac
     8221 aggtttaagg cttttattgg ttgaaactgg tggaacaatt ttagcagcag caatgcctgg
     8281 aaaaattaaa acttttagtc agatgtttgc tattattttc ttgctattac attggacttt
     8341 gcttggtcaa gttctacttt atgtagcctt atttttcact atctactctg gctatgacta
     8401 tttcaagggt agtgcctatg tatttaaagg gacatttggt tcgaaatgaa atcaataatt
     8461 gatgtaaaaa atctttcttt tcgctataaa gaaaatcaga actactacga tgtgaaggat
     8521 attacgtttc acgtgaaacg tggagaatgg ctttcgattg tagggcataa tggtagtggt
     8581 aaatcaacga cggttcgatt aattgatggc ttactggaag cagaatccgg agagattgta
     8641 attgatggcc aacgtttgac tgaggaaaat gtttggaata tacgtcgtca aatcggtatg
     8701 gtttttcaaa atccagacaa tcaatttgtt ggagcgactg ttgaagatga tgttgctttt
     8761 ggtttggaaa atcagggact ttctcgtcaa gaaatgaaaa agagagtgga agaagctctg
     8821 gatttagttg gcatgttgga ctttaaaaag agagagccag cgcgtctatc aggtggccaa
     8881 aagcaacgtg tggccattgc aggtgttgta gccctaagac cagctatttt aatcttagat
     8941 gaagcaacga gtatgttgga tcctgagggg cgtagagaac ttattgggac agtaaaagga
     9001 attcgaaaag actatgatat gacggtcatt tctattaccc atgatttgga agaagtcgcc
     9061 atgagtgatc gcgtattggt catgaaaaaa ggggaaattg aatcaactag tagtccaagg
     9121 gagcttttct ctcgaaatga tttagatcaa attggattag acgatccttt tgctaatcaa
     9181 ttaaaaaaat ctttgagcca gaatggctat gatttacctg aaaattattt gacagaaagt
     9241 gagctagagg ataagctatg ggaattgctc tagaaaatgt gaattttaca tatcaagaag
     9301 gtactccctt agcttcagca gctttgtcgg atgtttcttt gacgattgaa gatggctctt
     9361 atacagcttt aattgggcac acaggtagtg gtaaatcaac tattttacaa ctcttaaatg
     9421 gtttattggt gccaagtcaa gggagtgtga gggtttttga taccttaatc acctcgactt
     9481 ctaaaaataa agatattcgc caaattagaa aacaggttgg cttggtattt cagtttgctg
     9541 aaaatcagat ttttgaagaa acggttttga aggacgttgc ttttggaccg caaaattttg
     9601 gagtttctga agaagatgct gtgaagactg cgcgtgagaa actggctctg gttggaattg
     9661 atgaatcact ttttgatcgt agtccgtttg agctgtcagg gggacaaatg agacgtgttg
     9721 ccattgcagg catacttgcc atggagccag ctatattagt cttagatgag ccaacagctg
     9781 gtctagatcc tctagggaga aaagagttga tgaccctgtt caaaaaactc caccagtcag
     9841 ggatgaccat cgtcttggta acgcatttga tggatgatgt tgctgaatat gcgaatcaag
     9901 tctatgtaat ggaaaaggga cgtttagtaa aggggggcaa accaagtgat gtctttcaag
     9961 acgttgtttt tatggaagaa gttcagttgg gagtacctaa aattacggcc ttttgtaaac
    10021 gattggctga tagaggcgtg tcatttaaac gattaccgat taagatagag gagttcaagg
    10081 agtcgctaaa tggatagtat gattttaggg cgttatatcc caggggattc gattgttcac
    10141 cgattggatc cacgtagcaa attgcttgct atgatgctac tgattttgat tgtgttttgg
    10201 gctaataacc ccttgacgaa tctgattctt tttatagcga cagggatatt tattgccttg
    10261 tcaggagtat ctctttcatt ttttattcag ggcttgaagt ctatgttttt cttgattgcc
    10321 ttcacaacta tttttcaact atttttcatt tctaatggga atgttttatt tgagttttcg
    10381 tttgtgagaa tcacggatta tgctttgcaa caagctggga ttattttttg tcgctttgta
    10441 ttgattattt tcttttcaac tttgttaacc ttaacaacca tgcctttaag tttggcatca
    10501 gctgtcgaag ctttattagc acctttaaag cgtgtgaaag ttccagttca tgaaattgga
    10561 ttgatgctgt ctatgagttt gcgttttgtc ccaaccttga tggatgatac gactcggatt
    10621 atgaatgcac agaaagctcg tggagtggat tttggagaag gaagtatcgt tcaaaaagta
    10681 aaggcgatga ttcccatttt gattcctctt tttgcgacaa gtttaaaacg tgcagattcc
    10741 ttggctatcg ctatggaagc gcgtggctat cagggtggaa aaggcagaag tcaataccga
    10801 caattgaaat ggactctaaa ggatacgctg accattcttg ttattctcgt acttggttgt
    10861 tgtttatttt tcttaaaatc ttagtagctt tcaggaattg ataaaaagtt actgtaacag
    10921 tttttgatat aaaggtaggg atatgaaccg ttttaaaaaa tcaaaatatg tcattattgt
    10981 ttttgtcact gttctgcttg tgtcagctct cttagcgacg acttattcaa gtacaattgt
    11041 gacaaaatta ggagatggaa tctcattggt tgatagagtt gtacaaaaac cttttcagtg
    11101 gtttgattct gtcaaatcag atttggctca tttgacacga acatataatg aaaatgaaag
    11161 tttgaagaaa cagctttacc aattagaagt taaatcaaat gaggtggaaa gtttaaagac
    11221 agaaaatgaa caactgcgcc aattgcttga tatgaagtct aaattgcaag ccacaaagac
    11281 tttagcagca gatgttatta tgcgttctcc ggtatcttgg aagcaggagt tgaccttaga
    11341 tgcaggtaga tcaaaaggtg cttctgagaa catgttagct attgcaaatg gtggcttgat
    11401 tgggagtatt tcaaaagtag aggagaactc tactatagtc aaccttctga caaatacgga
    11461 aaatgctgat aagatttctg ttaaaattca acatggctct actacaattt atggaattat
    11521 tattggctat gacaaggaaa atgacgttct taaaattagc caattaaata gtaatagcga
    11581 tattagtgcg ggagataagg tgactactgg tggattagga aactttaacg ttgctgatat
    11641 tcctgttggt gaagtggttg ccacaacgca tagtacagac tatttgacac gagaagtaac
    11701 tgttaaattg agtgcagata ctcataatgt agatgtgata gaattagtgg ggaattcata
    11761 atgagacagt tgaagcgagt tggagtattt ttattgcttc ctttctttgt tctaattgac
    11821 gcccatatta gccagcttct gggctcattt ttcccccatg tacatttggc tagtcatttt
    11881 ctttttctat ttctcttatt tgagacgata gaagtatcag agtatctcta cctagtctat
    11941 tgttttgtta taggcttggt ttatgatgtt tactttttcc atctaatagg gattacaact
    12001 ctcttattta tcttattggg agccttcctt cataaattga atagtgttat tttgttgaat
    12061 cgttggacaa gaatgctagc tatgattgtg ctgacattcc tgtttgaaat gggtagttat
    12121 cttttggctt ttatggt
//
LOCUS       DAVKDZ010000060        12113 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000060 DAVKDZ010000000
VERSION     DAVKDZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12113
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.061"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            176..1306
                     /locus_tag="VWZ30_001598"
     CDS             176..1306
                     /locus_tag="VWZ30_001598"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU9335298.1"
                     /translation="MKKRSETIKYRRIVFKVGTSSLTNEDGSLSRSKVKAITQQLAML
                     HESGHELILVSSGAIAAGFGVLGFKKRPTKITDKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLSRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVLLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            1316..2578
                     /locus_tag="VWZ30_001599"
     CDS             1316..2578
                     /locus_tag="VWZ30_001599"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU9335299.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTVL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHEDKAASFLPRLDQ
                     MLVAERKEVGLEPIQFRLDSKASQFVSGQVAEAQDFDTEFLDYVLAVKVVSSLEEAVT
                     HIETHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            2582..3379
                     /gene="proC"
                     /locus_tag="VWZ30_001600"
     CDS             2582..3379
                     /gene="proC"
                     /locus_tag="VWZ30_001600"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU9335300.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDELLLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFCQLLAKAGLLVGLGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEVHAFRGTVMDAVHQAYKRTQELGQ"
     gene            3599..4237
                     /locus_tag="VWZ30_001601"
     CDS             3599..4237
                     /locus_tag="VWZ30_001601"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU9335301.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLLILEEKGVEVLTSREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            4234..5124
                     /locus_tag="VWZ30_001602"
     CDS             4234..5124
                     /locus_tag="VWZ30_001602"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU9335302.1"
                     /translation="MKQEQLKAWQPAQFDRFVRILEQDQLNHAYFFSGFFGSLEMAQF
                     LAKSLFCTDKVDVLPCENCRNCKLIEQGEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEQLILLLEQMGLVKKKATLLAQFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRVLEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            5164..5481
                     /gene="yabA"
                     /locus_tag="VWZ30_001603"
     CDS             5164..5481
                     /gene="yabA"
                     /locus_tag="VWZ30_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEU9335303.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            5484..6353
                     /gene="rsmI"
                     /locus_tag="VWZ30_001604"
     CDS             5484..6353
                     /gene="rsmI"
                     /locus_tag="VWZ30_001604"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU9335304.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKVGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     SETSLKGECLLIVEGASQDVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            6571..7626
                     /locus_tag="VWZ30_001605"
     CDS             6571..7626
                     /locus_tag="VWZ30_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU9335305.1"
                     /translation="MKKYNIVDLFSGAGGLSYGFEMAGFNVLLGIDNDEKALETFQKN
                     HQNSEILCGDIANISYEEDIKPIIGEQKIDIIVGGPPCQGMSLSGPRRFDDPRNKLYL
                     SYIRLVREIQPTAFVIENVIGIVSLFKGQIKDTIIEEFSKMGYKVQFKVLLASDYGVP
                     QNRKRVIFVGTRNDGFEYPEALGTIITTEMAISDLPTLENELGEIEMSYVSEPQNDYQ
                     KLMRKHSNVVLNHVAAKHSEKVISTIALVPDGGNYKNLPKELRETRKFNVAWTRFASW
                     KPAPTIDTGHRHHFHYKYNRVPTVRESARLQSFPDDFIFYGSKTQQFRQVGNAVPPLM
                     AQQIANNLLKYLKNNEE"
     gene            7629..9029
                     /locus_tag="VWZ30_001606"
     CDS             7629..9029
                     /locus_tag="VWZ30_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEU9335306.1"
                     /translation="MYQVPEKYYFRLHHPRPRFKNDVENVLVYMATNISYLGILPKKE
                     FSQQLNDIIKQYAGNADKTEKTINNWRTEISSLFGMMIEKEGFVYSGNRAKELTEDQD
                     LIRFFKTFLYNFEYPGGHLKDNEIVKLCQIGVQFKPAQYLLELFTEASQAEEKTIYLT
                     DEEVAHCIFNDLRCTRDREPVLEIWNRIKENRASKVAYDTKGDVIRYAKDVLDYMVIA
                     SLLTVQGGRYKINSMEEQATNDFLQSDATFDGYAQFIGNASTTIEDVRNFRSAWFEYV
                     NRDLGDIDFRTNFNLYLPSDIDFTEEKTDIIGHLTEHIEYSEKISTAVVGSSGEAIVI
                     NHEKQKLREHGQEQLLHLVNFIPTQFGVGYDVQSFECDDSELRKYIEVKTTVSNASIT
                     FNSFHITPNEWRTAKSVRDRYYIYRLQISKHSKKLFIIKNLYELVKNEKMLLIERKDG
                     YDARFSSSVGFEENLI"
     gene            9026..10066
                     /gene="dcm"
                     /locus_tag="VWZ30_001607"
     CDS             9026..10066
                     /gene="dcm"
                     /locus_tag="VWZ30_001607"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEU9335307.1"
                     /translation="MKKLKIASLFSGGGGTDIGFAGGFDFLGKHYADNQIEIVYANDI
                     EDSANKMFEKNFGVTPDNRNIREIKSDEIPSFDILTGGFPCQSFSVSAQNPKRLGIKD
                     EKGTLFFEMVRVLKAHQPKAFFAENVKGILSANNKEAFPLIIEEFEKAGYNVSYKLCV
                     ATKYGVPQKRERVFIVGIRKDLNFTFEFPEEPIQNVEDYTPLKVILQQDIDEKYYFSE
                     RAVQGMLNSKSGKKMNKGRAQDIEQPSNTVSSHLAKVSLNSTDPVLLENGRYRRYTPR
                     EVARIQSFPENYELLGSEGAQYRMLGNAIPPVMMWYIAEKLVSDLRNSDVIAKQQKLE
                     ESFGQLDLFEAS"
     gene            10091..10885
                     /locus_tag="VWZ30_001608"
     CDS             10091..10885
                     /locus_tag="VWZ30_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEU9335308.1"
                     /translation="MELNFQDKSFIKVFFNSDGYVLNFSNSTFANFTFNSVGVNIQEK
                     YGGSKGKALQAFVDNEPDELVLKLALDLLRYYEDLPDSYYEKTEERNSQALKLKERLS
                     KYEVSQIAYLNNAAKSLSDYFDSDYIDKQMGIMNEMISRSPADAIGKAKELLESCFKH
                     ILDHEKIEYSNSNDIATLQKKVFKFLNLDSNENISAKNNQDVKVVLSGLNQIIKGINN
                     LRNDKGDGHGKGANFIELPPRYASVVVGSALTVVSFVWETYQDKQK"
     gene            10903..11466
                     /locus_tag="VWZ30_001609"
     CDS             10903..11466
                     /locus_tag="VWZ30_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HEU9335309.1"
                     /translation="MSKNQEKLHFQLKKFIVNVGWTHKIHAVRIDELESYIRWFRIAT
                     IIISGVVSSGLVGILWFDEYWIKLVTAFLSLVTTIIFSITKEFNFEERLALERKSVDE
                     LWNLRVLAEILLSEVVYNGKPSSEIQEFFEELKFRRDATYSQLSNASPKNVSKASKLI
                     KSRKDNDYEEDYRYFIPKELMEIKEEE"
     gene            11466..>12113
                     /locus_tag="VWZ30_001610"
     CDS             11466..>12113
                     /locus_tag="VWZ30_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU9335310.1"
                     /translation="MSLSNDFKEFCEDELPMTDEEWEKWENRLKEITKRLNRKFYSLD
                     ELDDQETDNGLIVGSVGRTTAVRNVSDFDYIFSLPSEVYRRFDSYEGNGQSALLQEIK
                     NELLKRYPNTKIKGDGQVVVIDFSDGGKIELVPAFEKSDGNFKYPDSRNGGSWKTTKP
                     KFEIEKCKSLNSNFNSHFSNLTRLIRQWKNHMGFSFKGLLIDTLISDLIDDKGISE"
BASE COUNT         3822 a         1969 c         2734 g         3588 t
ORIGIN      
        1 tctttctaat gagttgtttt gtcgcttttc attataggtc atatgggact ttttttctac
       61 acaaaaatag gctccataat atccataggg gatttaccca ctacaaatat tatagagcct
      121 ctttttccta tcaactcttt tctatttcct gttattcatg ttataatgga taaatatgaa
      181 gaaacggagt gagactataa aatacagacg gattgtcttt aaggttggga cttcttctct
      241 gacaaatgag gatggaagtt tatcacgtag taaggtaaag gctatcaccc agcagttggc
      301 tatgttgcac gagtctggtc atgaattgat tttggtgtcg tcaggtgcca ttgcagctgg
      361 ttttggggtc ttaggattta aaaagcgtcc gactaagatt actgataaac aggcttcagc
      421 agcggtagga caagggcttt tgttggaaga atacacgact aatcttctct tgcgccaaat
      481 cgtttctgca caaatcttgc tgacccagga tgattttgtg gataagcgcc gttataaaaa
      541 tgcccatcag gctttgtcag ttctacttag ccgtggggca attcctatta tcaacgagaa
      601 cgatagtgtc gttattgatg agctcaaggt tggggacaat gatactctga gtgcccaggt
      661 agcggccatg gtccaagctg accttttggt cctcttgaca gatgtagacg gtctctatac
      721 tggaaatcct aattcagacc caagagccaa acgcttggag cgaatcgaga ccatcaatcg
      781 tgagattatt gatatggctg gtggagctgg ttcgtcaaac ggaactgggg gcatgctaac
      841 aaaaattaag gctgcaacta tcgcgacgga atcaggagtt cctgtttata tctgctcatc
      901 cttgaaatca gattccatga ttgaggcggc agaggagacc gaggatggtt cttactttgt
      961 tgctcaagag aaggggcttc gtacccagaa acaatggctt gccttctatg ctcagagtca
     1021 aggttctatt tgggttgata aaggggctgc ggaagctctc tctcaacatg gaaagagtct
     1081 tctcttatct ggtatcgttg aagcagaagg agccttttct tacggtgata tcgtgacagt
     1141 atttgacaag gaaagtggaa aatcacttgg aaaaggacgc gtgcaatttg gagcatctgc
     1201 tttggaggat atgttgcgtt ctcaaaaagc caagggtgtc ttgatttacc gtgacgactg
     1261 gatttccatt actcctgaaa tccaactact ttttacagaa ttttagaggt aaactatggt
     1321 gagtagacaa gaacaatttg aacaggtaca ggctgttaaa aaatcgatta acacagctag
     1381 tgaagaagtg aaaaaccaag ccttgctagc catggctgat cacttagtgg ctgctactga
     1441 ggaaatttta gcggctaatg ccctcgatat ggcagcggct aaggggaaaa tctcagatgt
     1501 gatgttggat cgtctttatt tggatgcaga tcgtatagaa gcgatggcaa gaggaattcg
     1561 tgaagtggtt gccttaccag atccaatcgg tgaagtttta gaaacaagtc agcttgaaaa
     1621 tggtttggtt atcacaaaaa aacgtgtagc tatgggtgtc atcggtatta tctatgaaag
     1681 ccgtccaaat gtgacgtctg atgcggctgc tttgactctt aagagtggaa atgcggttgt
     1741 tcttcgtagt ggtaaggatg cctatcaaac aacccatgcc attgtcacag tcttgaagaa
     1801 gggcttggag acgactacta ttcatccaaa tgtgattcaa ctggtggaag atactagccg
     1861 tgaaagtagc tatgccatga tgaaggccaa gggttatcta gatcttctca ttcctcgtgg
     1921 aggagctggc ttgatcaatg cagtggttga gaatgcgatt gtacctgtta tcgagacagg
     1981 gactgggatt gtccatgtct atgtggataa ggatgcagac gaagacaagg cgctgtctat
     2041 catcaacaat gctaaaacca gtcgtccttc tgtttgtaat gccatggagg ttctccttgt
     2101 tcatgaagat aaggcagcaa gcttccttcc tcgcttggat caaatgctgg ttgcagagcg
     2161 taaggaagtt ggactggaac caattcaatt ccgcttggat agtaaagcaa gtcagtttgt
     2221 ttcaggtcaa gtagctgaag cacaagactt tgacaccgag tttttagact atgtcctagc
     2281 ggttaaggtt gtgagcagtt tagaagaagc ggttacccat attgaaaccc acagtaccca
     2341 tcattcggat gccattgtaa cggaaaatgc tgaagctgca gcttacttta cagatcaagt
     2401 ggactctgca gcggtgtatg ttaatgcctc aactcgtttc acagatggag gacaatttgg
     2461 tcttggttgt gaaatgggga tttctactca gaaattgcac gcgcgtggtc caatgggctt
     2521 gaaagagttg accagctaca agtatgtggt tgctggtgat gggcagataa gggagtaaga
     2581 gatgaagatt ggatttatcg gtttggggaa tatgggtgct agcttggcaa aatctgtctt
     2641 gcagactagg acgtcagatg agcttctcct tgccaatcgt agtcaagcca aggtagatgc
     2701 tttcattgca gactttggtg gtcaggcttc cagcaatgaa gaaatgtttg cagaagcaga
     2761 tgtgattttt ctaggagtta agcctgctca gttttctgaa ctgctttctc aataccagac
     2821 catccttgaa aaaagagaaa gtcttctttt gatttcgatg gcagctggat tgaccttaga
     2881 aaaactagca agtcttatcc caagtcaaca ccgaattatt cgtatgatgc ccaatacccc
     2941 tgcttctatc gggcaaggag tgattagtta tgccttgtct cctaattgcc gggctgagga
     3001 cagtgagctc ttttgtcagc ttttagccaa ggctggtctc ttggttggat taggagaaag
     3061 cttaatcaat gcagcgacag gtcttgcagg ttgtggacca gcctttgtct atctctttat
     3121 tgaggccttg gcagatgcgg gtgttcagac gggattacca cgagaaacag ccttgaaaat
     3181 ggcagcccaa actgtggtag gagctgggca attggtccta gaaagccagc aacatcctgg
     3241 agttttgaaa gatcaagttt gtagtccagg cggttcgact attgccggcg tagcaagcct
     3301 agaagtgcat gcttttcgag gaacggtcat ggatgcagtt catcaagcat ataaacgaac
     3361 acaagaacta ggtcaataag aggtagtttt atctgcctct tttatggtgg ttgatactct
     3421 tcgaaaatca aattcaaact gcgtcagctt caccttgccg tactcaagta cagcctgcgg
     3481 ctagcttcct agtttgctct ttaattttca ttgagtatga aatgagaaga caaaaagatt
     3541 gtcacaaacc cctatttttt tgatagaata gaagtagtaa aaaagaaatg agttagacat
     3601 gtcaaaagga tttttagtct ctcttgaggg accagaggga gcaggcaaga ccagtgtttt
     3661 agaggctcta ctactaattt tagaggaaaa aggagtagag gtgctgacga gccgtgagcc
     3721 aggcggtgtc ttgattgggg agaagattcg ggaagtaatt ttggatccaa gtcatactca
     3781 gatggatgcg aagacagagt tgcttctcta tattgccagt cgcagacagc acttggtgga
     3841 aaaagttctt ccagcccttg aagctggcaa gctggtcatt atggatcgtt ttatcgatag
     3901 ttctgttgcc tatcagggat ttggtcgtgg cttagatatt gaagccattg actggctcaa
     3961 tcagtttgcg acagatggcc tcaaacccga tttgacactc tattttgaca tcgaggtgga
     4021 agaaggactg gctcgtattg ctgctaatag tgaccgcgag gttaatcgtt tggatttgga
     4081 agggttggac ttgcataaaa aagttcgtca aggctacctt tctcttctgg ataaagaggg
     4141 aaatcgcatt gtcaagattg atgctagtct ccctttggag caagttgtgg aaactaccaa
     4201 ggctgtcttg tttgacggaa tgggcttggc caaatgaaac aagaacaatt aaaggcttgg
     4261 caaccagccc agtttgaccg atttgtccgt atcttagaac aagaccagct caatcacgcc
     4321 tatttctttt caggtttctt tggaagcctg gaaatggcgc agtttttggc caagagtcta
     4381 ttttgtacgg ataaggtaga tgttttacca tgtgaaaact gccgaaattg taagctgatt
     4441 gaacagggag agtttccaga tgtaaccttg attaagccag tcaatcaggt catcaagaca
     4501 gaacgcattc gggaattggt gggacagttt tctcaagcag ggattgaaag ccagcaacag
     4561 gtctttatca tcgagcaagc ggataaaatg catcccaacg cagccaattc tctgcttaag
     4621 gtcatcgaag aaccccagag tgaagtttat attttcttct tgactagcga tgaggaaaag
     4681 atgttaccga caatccgaag tcggacccag attttccact ttaaaaagca agaagaacaa
     4741 ctcatcttgc tcttagaaca aatgggactt gttaagaaaa aagcgactct tttagctcag
     4801 tttagtcaat cgcgagctga agcagaaaag ttggctaatc aggcaagttt ttggaccttg
     4861 gtcgatgaaa gtgaacgcct gctgacttgg ttagtagcta agaaaaaaga aagttatcta
     4921 caggttgcca aattagcgaa cttggcagat gataaggaaa aacaagatca ggttttacgg
     4981 gttcttgaag ttctctgtgg gcaggacctc ttgcaggtaa gagtaagggt gattctacaa
     5041 gatttgctag aagctagaaa aatgtggcaa gctaatgtca gctttcaaaa tgccatggaa
     5101 tatctggtct tgaaagaaat ataaactcaa aaatgaacga taaagaaagg aaaggcctgg
     5161 cttatggaca aaaaagaatt atttgacgcg ctggatgatt tttcccaaca gttattggta
     5221 accttggccg atgtggaagc tatcaagaaa aatctcaaga gcctagtaga ggaaaataca
     5281 gctcttcgtt tggaaaattc taagttgcgc gaacgtttgg gtgaggtgga agcagacgct
     5341 cccgtcaagg ccaagcatgt tcgcgaaagt gttcgtcgca tttaccgtga tggatttcac
     5401 gtatgtaatg atttttatgg acaacgtcga gaacaggacg aggaatgtat gttctgtgac
     5461 gagttgttat acagggagta agcatgcaga ttcaaaaaag ttttaagggg cagtctccct
     5521 atggcaagct gtatctagtg gcaacgccga ttggcaatct agatgatatg acctttcgcg
     5581 ctatccagac cttgaaagaa gtggactgga ttgctgctga ggatacgcgc aatacaggtc
     5641 ttttgctcaa gcattttgac atttccacca agcagatcag ttttcatgag cacaatgcca
     5701 aggaaaaaat tcctgatttg attggtttct tgaaagtagg gcaaagtatt gctcaggtct
     5761 ctgatgccgg tttgcctagc atttcagacc ctggtcatga tttggttaag gcagctattg
     5821 aggaagaaat tgcagttgtg actgttccag gtgcctctgc aggaatttct gccttgattg
     5881 ccagtggttt agctccacag ccacatatct tttacggttt tttaccgaga aaatcaggtc
     5941 agcagaagca attttttgac tcgaaaaaag attatcctga aacacagatt ttctatgaat
     6001 cacctcatcg tgtagcagac acgttggaaa atatgttaga agtctacggt gaccgctccg
     6061 ttgtcttggt cagggaattg accaaaatct atgaagaata ccaaagaggt acaatttctg
     6121 aattgctgga aagcatctct gaaacgtctc tcaagggtga atgtcttctg attgttgaag
     6181 gtgccagtca ggatgtggaa gaaaaagacg aggaagactt gttcttagaa atccaagctc
     6241 gtatccagca aggtatgaag aaaaatcaag ctattaagga agtagctaag atttaccagt
     6301 ggaataagag tcaactctac gcagcctacc acgactggga agaaaaacaa taaagggaga
     6361 caggatgtaa taattctgtc tgtttctgtt taacttaatt agtgatgata atattcggag
     6421 tctaaataat agaattgatt gagtagtgaa taaataaaaa caatgttcat tcagtttacg
     6481 ttgaatatat ataatgtact ctattagaat tatttttaag tacgcagtat ggtataatta
     6541 tatatgagaa aatagtgtgg agtttatcag ttgaaaaaat ataacattgt agatttattt
     6601 tcaggagcag gtggtctatc ttatggattc gagatggctg gatttaatgt tctattagga
     6661 attgataatg acgaaaaagc cttagaaacc tttcaaaaaa atcatcagaa ttcagaaata
     6721 ttatgtggag atattgcgaa tatatcttat gaggaagata taaaaccaat aattggagaa
     6781 caaaaaattg acataattgt aggtggtccc ccttgtcagg ggatgtcgct ttctggacct
     6841 agaagatttg atgatccaag gaacaaactt tatttgtctt atattcgatt ggttcgagaa
     6901 atacagccaa ctgcttttgt cattgaaaat gtgattggga ttgttagttt gtttaaaggg
     6961 caaataaaag atactatcat agaagaattt tcgaaaatgg gttataaggt tcagtttaaa
     7021 gtgttgctgg cctctgacta tggagttcct caaaatagaa aacgtgttat ttttgtgggg
     7081 acgaggaatg atggatttga atatcctgaa gcattgggaa caatcattac aacagagatg
     7141 gcaatttctg atttaccaac attagaaaat gaacttggcg agatagaaat gtcttatgtt
     7201 tcagaaccac aaaatgacta tcaaaaacta atgcgaaaac attcaaacgt agttttgaac
     7261 cacgttgcag ccaagcattc agaaaaagtg ataagtacaa ttgcccttgt ccctgacggt
     7321 ggaaattata aaaatctccc gaaggaatta cgagaaacaa gaaaatttaa tgtcgcttgg
     7381 acacgatttg ctagttggaa gccagctcct acgattgata cggggcatag acatcatttc
     7441 cactataagt ataatcgtgt gccaacagtg agggaatctg ctcgtctaca atcttttcca
     7501 gatgatttta tcttttacgg aagtaaaaca cagcaattta gacaagttgg taatgctgtt
     7561 cctcctttaa tggctcagca gattgcaaac aatttactga aatatttaaa aaataacgaa
     7621 gagtaactat gtatcaagtt ccagagaaat attattttag actacatcac ccaagacctc
     7681 gttttaaaaa tgatgtcgaa aatgtacttg tttatatggc aacgaatatt tcttatttgg
     7741 gaatattgcc taaaaaggaa tttagtcagc aactaaatga cattatcaaa caatatgcag
     7801 gaaatgcaga taaaacagag aaaacaatca ataactggcg aacagagatt tcttctttgt
     7861 ttggaatgat gattgagaaa gaaggctttg tctattcagg aaatagggct aaggaattga
     7921 ctgaagacca agaccttatt cgctttttta aaaccttttt gtataatttt gaatatcctg
     7981 gtgggcatct aaaagataac gagattgtta aactttgtca aatcggagtt caatttaagc
     8041 cagctcagta tctactggaa ctatttacag aagcctcaca agctgaagaa aaaactattt
     8101 acctcaccga tgaagaagtc gcccattgta tctttaatga tttgagatgt actcgtgata
     8161 gagagcctgt tttggaaata tggaatcgta tcaaagagaa tagagcgtct aaagtagctt
     8221 acgatactaa aggagatgtt attcgatatg ccaaagatgt tttagattac atggttatag
     8281 cgagtttact aactgttcaa ggtggtaggt ataaaataaa ttctatggaa gagcaagcaa
     8341 caaatgactt tttgcaatca gatgctacct ttgatggcta tgctcaattt attggaaatg
     8401 cttctacaac aattgaagat gttcgaaatt ttagaagtgc ttggttcgaa tatgtcaatc
     8461 gtgatttagg agatattgat tttaggacaa actttaatct ctaccttcca agtgatattg
     8521 attttactga agagaaaaca gatattatcg gacatctaac tgaacacatc gaatacagcg
     8581 agaaaatatc taccgctgtt gtagggagtt cgggagaagc tattgttatc aatcatgaaa
     8641 aacaaaaatt acgagaacat gggcaagaac agttgctcca tctggtgaat tttattccaa
     8701 cacagtttgg tgtcggctac gatgttcaat cttttgagtg tgatgattct gagctgagaa
     8761 agtatattga agtcaaaaca actgtcagca atgcttctat tacttttaat tcgtttcata
     8821 tcacaccaaa cgaatggcga actgctaaat ctgtgcgtga tagatactat atttatcgtt
     8881 tgcagatttc taaacatagc aaaaaattgt ttattatcaa gaatttgtat gagttagtca
     8941 aaaatgagaa gatgcttctg attgagagaa aagatggata tgatgcccgt ttttcatcgt
     9001 cagttggctt tgaggagaat ttaatttgaa gaaactaaaa attgcctctc tttttagtgg
     9061 agggggtgga acggatattg gttttgctgg tggatttgat tttttaggta aacattatgc
     9121 cgacaatcaa attgagattg tctatgcaaa tgatattgaa gacagtgcca ataaaatgtt
     9181 tgaaaagaat tttggtgtta ctccagacaa tcgaaatatc cgagaaataa aatcagatga
     9241 aatcccatct ttcgatattt taacgggagg ttttccttgt caatcatttt ctgttagtgc
     9301 tcaaaatccg aaacgcttag gaataaaaga cgaaaaaggg acattgtttt ttgagatggt
     9361 gcgagtgtta aaagctcatc aaccaaaagc attttttgcc gaaaatgtta agggaatttt
     9421 gtctgcaaac aataaagaag cctttcctct aattattgaa gagttcgaaa aagctggtta
     9481 taatgtttct tataaacttt gcgtagccac caaatacggc gttccgcaaa agagagagcg
     9541 tgtgtttatt gttggtattc gaaaggattt gaattttact tttgaatttc ctgaagagcc
     9601 tattcaaaat gtagaagatt atacaccact aaaagtaatt cttcagcaag atattgatga
     9661 aaagtattat tttagtgaga gggctgtgca aggaatgcta aatagtaaat ctggcaaaaa
     9721 aatgaataaa ggacgagcac aagacattga acagccgagt aatacggtta gttctcactt
     9781 agcaaaagta tccttaaata gcactgaccc tgttttgtta gaaaatggac gttaccgtcg
     9841 ctatactcca agagaggttg cacgtattca gtcgtttcca gaaaactatg aattgcttgg
     9901 ttcagaaggt gctcaatata gaatgctggg gaatgctatt ccaccagtaa tgatgtggta
     9961 tattgctgaa aagttagtgt cagatttacg aaatagtgat gtaattgcaa agcaacagaa
    10021 actcgaagaa agttttggtc aactggattt gtttgaagcg agttgatgtt ttaaatctag
    10081 ggaacttgat atggaattaa attttcaaga taagagtttt ataaaagtat tttttaatag
    10141 tgacggttat gtattaaatt tttctaactc tacttttgct aattttactt ttaattcagt
    10201 aggagtaaat attcaagaga aatatggagg ttcaaaaggg aaagctttac aggcttttgt
    10261 agataacgaa ccagatgaac tggttttaaa attggcttta gatttactta gatattatga
    10321 agaccttcct gactcatatt acgagaaaac tgaagagagg aactctcagg cattgaaatt
    10381 gaaagagaga ctttcgaaat atgaagtctc acaaatagcc tacttaaata atgctgctaa
    10441 atctttatct gactattttg atagcgacta tatagataag caaatgggga ttatgaatga
    10501 aatgatttct cgctctccag cggacgctat tggaaaagct aaggaattgt tggaatcgtg
    10561 ttttaaacat attttagacc atgaaaaaat tgagtattct aattctaatg atattgcaac
    10621 tttgcaaaag aaagtcttta aatttttaaa cttggattct aatgaaaata tttcagcaaa
    10681 aaataatcaa gatgtgaagg tggttctaag tggtttgaat caaattatca agggtattaa
    10741 caatcttaga aatgataaag gcgatggtca tggtaaggga gctaacttta tagaattgcc
    10801 accaaggtat gcttcagttg ttgtaggttc tgcattaacg gtagtgtcat ttgtttggga
    10861 aacataccaa gataagcaaa aatagtaaag gagggagatt taatgtcgaa aaatcaagaa
    10921 aaattacact ttcaacttaa aaaatttata gtaaatgtag gttggactca caaaattcat
    10981 gcggttagaa ttgacgagtt agaaagctat attcgatggt ttagaatagc aacaataatc
    11041 atttctggag ttgtctcttc tggcttagtt ggaatacttt ggtttgatga atattggata
    11101 aagcttgtta cagcattttt atcattagtc acgacaataa tttttagcat cacaaaggag
    11161 tttaactttg aggaacgact agctttggag aggaaatctg ttgatgagct ttggaatctt
    11221 agagtgttag ctgaaattct tttatctgag gtggtttaca atggaaaacc atccagtgaa
    11281 atccaagaat ttttcgaaga attaaaattt cgaagagatg ctacttatag tcaactttct
    11341 aatgcttctc ccaaaaatgt atccaaggct tcaaaactaa taaaatcgag aaaagataat
    11401 gattatgagg aggattatag atactttatt ccaaaagaat tgatggaaat taaggaggaa
    11461 gagtaatgtc tttatctaat gattttaaag aattttgcga agacgaactc cccatgacgg
    11521 atgaggaatg ggaaaaatgg gagaatagat taaaagagat tacgaaacga cttaatcgaa
    11581 aattttattc cttagatgag cttgacgatc aagaaactga caacggtttg attgttggta
    11641 gtgtggggag aacgacggct gttcgaaatg ttagtgattt tgattatatt ttttctctac
    11701 catcagaagt gtatagacga tttgattctt atgagggcaa tggtcaatca gcactcctac
    11761 aggaaataaa aaacgaatta ctaaaaagat acccaaatac aaaaataaaa ggagacggac
    11821 aggtcgttgt tattgatttt tcggatggtg gaaaaattga gttagttcca gctttcgaaa
    11881 aatctgatgg taattttaaa tatcctgatt ctcgtaacgg aggctcttgg aaaactacta
    11941 aaccaaagtt tgaaattgaa aaatgcaaat ctttaaattc caattttaat agtcattttt
    12001 ctaacttaac aaggctcata cgacaatgga aaaatcatat gggcttttca tttaaggggt
    12061 tgttgataga tactttgata tctgatttaa ttgatgataa aggtatttct gaa
//
LOCUS       DAVKDZ010000061        11932 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000061 DAVKDZ010000000
VERSION     DAVKDZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.062"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            124..2289
                     /locus_tag="VWZ30_001611"
     CDS             124..2289
                     /locus_tag="VWZ30_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9335311.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYQIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            2289..3029
                     /locus_tag="VWZ30_001612"
     CDS             2289..3029
                     /locus_tag="VWZ30_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9335312.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEKLGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            3178..4665
                     /locus_tag="VWZ30_001613"
     CDS             3178..4665
                     /locus_tag="VWZ30_001613"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU9335313.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKFFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRFE"
     gene            complement(4711..6000)
                     /gene="ftsY"
                     /locus_tag="VWZ30_001614"
     CDS             complement(4711..6000)
                     /gene="ftsY"
                     /locus_tag="VWZ30_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU9335314.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDIEPAFTEAEEVS
                     QEEAEVESSEESVFQEEDSQDTVEENLDLEPVVEVSQEEVEEFPNSQEVTEEEKLEHE
                     GTIEENNSEVLEPETPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVTEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(6004..6822)
                     /locus_tag="VWZ30_001615"
     CDS             complement(6004..6822)
                     /locus_tag="VWZ30_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9335315.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKATLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDDHEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYTKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPNNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAVANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            complement(6822..7616)
                     /locus_tag="VWZ30_001616"
     CDS             complement(6822..7616)
                     /locus_tag="VWZ30_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9335316.1"
                     /translation="MTIKLVATDMDGTFLDGSGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNEHVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            complement(7613..11152)
                     /gene="smc"
                     /locus_tag="VWZ30_001617"
     CDS             complement(7613..11152)
                     /gene="smc"
                     /locus_tag="VWZ30_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU9335317.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKYDKESSLALLEGNLVQNNQKLHRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVYYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLGSEEATLKTLQDEMARLTESLEAIKFQGEQARIQEQGLFLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKENCQERLATIARDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEQVAQARLLKTELQGQKRYEVADIERLGKELDNLDIEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEEAKMQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRYLQSQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKDRFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            complement(11143..11841)
                     /locus_tag="VWZ30_001618"
     CDS             complement(11143..11841)
                     /locus_tag="VWZ30_001618"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU9335318.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
BASE COUNT         3365 a         2704 c         2221 g         3642 t
ORIGIN      
        1 atagcaattc aagaaagaaa aggaaaaatc aatgttatat tttctgacac gaaaaatata
       61 aaaatttgcc tttttttact ttttctgata taatgagaga atattcggaa aaggagacta
      121 aaaatgaaga aaaaatttct agcatttttg ctaattttat tcccaatttt ctcactaggt
      181 attgccaaag cagaaacgat taagattgtt tctgataccg cctatgcacc ttttgagttt
      241 aaagattcag atcaaactta taaaggaatt gatgttgaca ttattaacaa agtcgctgag
      301 attaaaggct ggaacattca gatgtcctat cctggatttg acgcagcagt caatgcggtt
      361 caagctgggc aagccgacgc tatcatggca gggatgacaa agactaaaga acgtgaaaaa
      421 gtcttcacca tgtctgatac ttactatgat acaaaagttg tcattgctac tacaaagtca
      481 cacaaaatta gcaagtacga ccaattaact ggcaaaaccg ttggtgttaa aaacggaact
      541 gccgctcaac gtttccttga aacaatcaaa gataaatacg gctttactat taaaacattt
      601 gacactggtg atttaatgaa taacagcttg agtgctggtg ccatcgatgc catgatggat
      661 gacaaacctg ttatcgaata tgccattaac caaggtcaag acctccatat tgaaatggat
      721 ggtgaagctg taggaagttt tgctttcggt gtgaaaaaag gaagtaaata cgagcacctg
      781 gttactgaat ttaaccaagc cttggctgaa atgaaaaaag atggtagtct tgataaaatt
      841 atcaagaaat ggactgcttc atcatcttca gcagtgccaa ctacaactac tctagcagga
      901 ttaaaagcta ttcctgttaa ggctaaatat atcattgcca gcgattcttc ttttgcacct
      961 tttgttttcc aaaattcaag caaccaatac actggtattg atatggaatt gattaaggca
     1021 atcgctaaag accaaggttt tgaaattgaa atcaccaacc ctggttttga tgctgctatc
     1081 agtgctgtcc aagctggtca agccgatggt atcattgctg gtatgtctgt cacagatgct
     1141 cgtaaggcaa cttttgactt ctcagaatca tactacactg ctaataccat tcttggtgtc
     1201 aaagaatcaa gcaatattgc ttcttatgaa gatctaaaag gaaagacagt cggtgttaaa
     1261 aacggaactg cttctcaaac cttcctaaca gaaaatcaaa gcaaatacgg ttatcaaatt
     1321 aaaaccttcg ctgatggttc atcaatgtat gacagtttaa acactggtgc cattgatgct
     1381 gttatggatg atgaacctgt tctcaaatat tctatcagcc aaggtcaaaa attgaaaact
     1441 ccaatctctg gaactccaat cggtgaaaca gcctttgccg ttaaaaaagg agcaaatcca
     1501 gaactgattg aaatgttcaa caacggactt gcaaacctta aagcaaacgg tgaattccaa
     1561 aagattcttg acaaatacct agctagcgaa tcttcaactg cttcaacaag tactgttgac
     1621 gaaacaacgc tctggggctt gcttcaaaac aactacaaac aactccttag cggtcttggt
     1681 atcactcttg ctctagctct tatctcattt gctattgcca ttgtcatcgg aattatcttc
     1741 ggtatgttta gcgttagccc atacaaatct cttcgagtca tctctgagat tttcgttgac
     1801 gttattcgtg gtattccatt gatgattctt gcagccttca tcttctgggg gattccaaac
     1861 ttcatcgagt ctatcacagg ccaacaaagc ccaattaacg actttgtagc tggaaccatt
     1921 gccctctcac tcaatgcggc tgcttatatc gctgaaatcg ttcgtggtgg tattcaggcc
     1981 gttccagttg gccaaatgga agccagccga agcttgggta tctcttatgg aaaaaccatg
     2041 cgtaagatta tcttgccaca agcaactaaa ttgatgttgc caaactttgt caaccaattc
     2101 gttatcgctc ttaaagatac aactatcgta tctgctatcg gtttggttga actcttccaa
     2161 actggtaaga ttatcattgc tcgtaactac caaagtttca agatgtatgc aatccttgct
     2221 atcttctatc ttgtaattat cacacttttg actagactag cgaaacgctt agaaaagagg
     2281 attcgttaat ggcaaaatta aaaattgatg taaatgattt acacaagcac tatggaaaaa
     2341 atgaagtcct aaaaggaatt acgactaagt tctatgaagg agatgttgtt tgtatcatcg
     2401 gtccttcagg ttctggtaag tcaactttcc tccgtagcct caatctttta gaagaagtca
     2461 ctagcggtca catcactgtg aacggctatg atttaactga aaaaacaacc aatgttgacc
     2521 acgtccgtga aaatatcggc atggtattcc aacacttcaa cctcttccct catatgtctg
     2581 tattggacaa catcaccttt gctcctattg agcacaagtt gatgactaag gaagaagctg
     2641 agaaattggg aatggagttg cttgaaaagg ttggactagc agataaagct aatgccaatc
     2701 cagatagcct atcaggtggt caaaaacaac gtgtggccat agctcgtggc ctagcaatga
     2761 atccagacat catgctcttc gatgaaccaa cttctgccct tgaccctgag atggttggag
     2821 acgtacttaa cgttatgaag gaattggctg agcaaggcat gaccatgatt atcgtaaccc
     2881 atgagatggg atttgctcgt caggttgcca accgcgttat ctttactgca gatggcgagt
     2941 tccttgaaga cggaacacct gaccaaatct ttgataaccc acaacaccct cgtctgaaag
     3001 agttcttaga taaggtctta aacgtctaaa ctcaaactgt aaggatttcc ttgcagtttt
     3061 tctacctcgt attggaattt ttgatttttc ggaaaattat gttagaatta agtttatgaa
     3121 atgaggtttc ctcataccta gcaagactag gaataaaaat agaaattagg tagctagatg
     3181 tcatctaagg ttattgttac aattttcggt gcgagtggag acctggctaa acgcaagctc
     3241 tacccttccc tttttagatt atataaatcc ggcaatcttt ccaagcactt tgccgttatt
     3301 ggaactgccc gtagaccttg gagtaaggaa tattttgaat ctgtagttgt cgagtccatc
     3361 cttgatttgg cagatagtac cgagcaagcc caagaatttg ctagccactt ctactatcaa
     3421 agccatgatg tcaatgattc ggaacattat attgctttgc gtcaattaca agctgagctt
     3481 aatgaaaaat accaagctga acacaataag ttcttcttct tgtctatggc acctcagttc
     3541 tttggaacca ttgccaaaca cctcaaatct gaaaacattg tcgatggcaa aggttttgag
     3601 cgcttgatcg ttgaaaaacc atttggtaca gattacgcaa ctgcaagcaa gttgaatgac
     3661 gaactcctag caacatttga cgaagaacaa attttccgta tcgaccatta tcttggtaag
     3721 gaaatgatcc aaagcatctt tgcagttcgc tttgcaaact tgatttttga aaacgtttgg
     3781 aacaaggatt ttatcgacaa tgttcaaatt acctttgcgg agcgcttggg tgtagaagaa
     3841 cgtggtggct actatgacca atccggtgcc ctccgtgaca tggtccaaaa ccacactcta
     3901 caacttcttt cgctcctcgc catggacaaa ccagcaagct tcacaaaaga cgagattcgt
     3961 gctgaaaaga ttaaggtctt taaaaacctc tatcatccaa ctgatgaaga actcaaagaa
     4021 cactttatcc gtgggcaata ccgctctggt aagattgatg gcatgaaata catctcttat
     4081 cgtagcgagc caaatgtgaa tccagaatca acaactgaaa cctttacatc tggtgccttc
     4141 tttgtagaca gcgatcgatt ccgtagtgtt cctttctttt tccgtacagg taaacgactg
     4201 actgaaaaag gaactcatgt caacatcgtc tttaaacaaa tggattctat ctttggagaa
     4261 ccacttgctc caaatatttt gactatctat attcaaccaa cagaaggctt ctctcttagc
     4321 ctaaatggga agcaagtagg agaagaattt aacttggctc ctaactcact tgattaccgt
     4381 acagatgcga ctgcaactgg tgcttctcca gaaccatacg aaaaattgat ttatgatgtc
     4441 ctaaataaca actcaactaa ctttagccac tgggatgaag ttggtgcgtc atggaagttg
     4501 attgaccgta ttgaaaagct ctgggctgaa aatggtgccc cacttcatga ctataaagct
     4561 ggaagcatgg gacctcaagc cagctttgac ctacttgaaa aattcggtgc caaatggact
     4621 tggcaaccgg atatcgccta tcgtcaagat ggtcgtttcg aataaaaaaa tttcctgcaa
     4681 gtttatgcct tgcaggattt ttgcttctga ttagattaaa ccttccaaga gacctttcat
     4741 aaagttttct gagttaaact ctccaatatc atcgattttt tcaccaaaac caatcaattt
     4801 tacaggaata ttgagttctt cacgaatggc tagaaccaca cctcctcgag cagttccatc
     4861 aatcttagtc aaaacaattc cggttaaagg tgtgattttc gaaaattctt tggcctgtac
     4921 tagggcattt tgacctgttg atgcatcaag tgccaagaag gtttcatgtg gtgcttctgt
     4981 cacaacacgt ttgataatac gaccaatctt ttccaactca gccataaggt tatccttatt
     5041 ttgcagacga ccagcagtat caatcatgag aatatcgata ccttcagcca cggcacgttc
     5101 cataccatca aagaccacgc tggctggatc agctttttca ggtccagtta ctactggaac
     5161 atctactcgt cggccccatt cagctagctg agctactgca cccgcacgga aggtatctgc
     5221 tgcaaccagc atgaccttct taccagctcg tttgtagcgg tgggctagtt ttccgataga
     5281 agttgttttc ccaacaccat tcacaccaac aaagagcata actgtcaagt tatcttggaa
     5341 gtggatgctt tcatcgtagc taccatcctt ttcataaagc tcaaccaatt tctcaatgat
     5401 gacacgacga agtgcatcag gtttcttcgc attttcaagc ttggcttcgt aacgtagttc
     5461 ctccgttaag ttagaagcga cttggacacc aacatcactc ataatcagca gttcttccag
     5521 ttcctcgaaa aattcttcgt caacagagcg gaagttagca aagaaggcat tcaagcgggc
     5581 accgaaacct gtgcgagttt tcttaagact gcggtcatat ttttcctgaa cagtttcttc
     5641 tgtttgagga gtttctggtt caagaacttc agaattattt tcttctatag ttccttcgtg
     5701 ctcaagcttc tcttcctctg tgacttcttg tgagtttgga aattcttcta cttcttcttg
     5761 agaaacctct acaactggct ctaaatccag attttcttcg actgtgtctt gactatcctc
     5821 ttcttggaac acagattctt cagaactttc aacctctgct tcttcttgag aaacttcctc
     5881 agcttctgtg aaggcaggct caatatcttc agacaaatca agattttcca gagcttcttt
     5941 tacaacttct tcgattttag gttcttcttt ttttccgaat agacggtcaa ataatcccat
     6001 atcttagttc tcctttagca catattcttc gatagcccag gcgacagctt cttcatcatt
     6061 ggtcatagga gttaccacat ttgcgactgc cttgacttca ggaacagcgt tttgcatggc
     6121 aacaccgaga cctgcccatt caatcataga gaggtcattg gcctcgtcac cacaagccat
     6181 cacttggctt tggtcgattc caagatgact gattagtttt gccaaacctg ttgctttatg
     6241 aacattattt ggtgaccatt ctagcaacat ttcacgtgat ttaaagattt catattggtc
     6301 aaacaattct ggagaaatct tctgaatggc tgcatccaag ggttcttgag caaaggcagt
     6361 cacgcatttg ttgtaggtca tttgactaga taagtcttca aagtccactg gaacaaaggt
     6421 caaggctgga ttgaatttgg tataaagact ttcttggtcc gattgaattt gataaacagt
     6481 tccttctgag atagcgtcaa gaggcagtga taatttctct gtttcttcat acaaacgtgc
     6541 cacatcatca tatgaaaaga ctgttttatc aaggatttct cctgtatttt tctgaactaa
     6601 accaccatta aaagtaatcg tatactcatc ttcgtggtcg tcagttccta gctcatggag
     6661 aaagaaatcc atggctttta agggacgacc agttgtcaat acgaccttga taccacgatc
     6721 acgcgcagct ttcaaagttg ccttggtgcg atccgtcagc cttttatcag tagtcagcaa
     6781 ggtaccgtcc aagtccaatg cgattaattt tatatctgcc attataagcc ctccatataa
     6841 gctataaccg actggtcctt atggtgacca atcacagtct ttgctaattc taaaatttca
     6901 ggtcgtgcat tttcaggagc tacaggatgt cccacaacct gcatcatatg taagtcatta
     6961 agattgtctc caaaagccat gacctgatcc attgtgatac caagtttttt agctaattca
     7021 acaatagcca cacccttatc aacatagtcc agaacaatat caatggattc aaagccagtt
     7081 gtcatggcct taacaccagg aacatgttcg tttacccaag cttccccagc ttcaagagtt
     7141 tcttctgtga agttggttgt aaatttgaaa atgtcatctg tgatatcttc caaactcgct
     7201 actttttgga tattttcatt atagtgctga ctcactttca aataggtctc atcaaccgta
     7261 tctagcacat aagaaccctt cttacccgtc aagagcagtt tattgatatc tacataaggt
     7321 gacgttttca gcttttcaaa agttgccaga taaaagtcac gagacatagt cgcttcatac
     7381 aagtcctgac cttgatactc taccaaactg ccattttccg cgatgaaaat aatgtcatca
     7441 cgaacaccag caaataattt ttccagagac agaaatcccc gacccgaagc caccgcaaag
     7501 taaatccctt tttccttgta ggaagccaag agagacttga gacgatccat atcaaagcgc
     7561 ccactcccat ctaggaaggt tccgtccata tccgttgcta ctagtttaat tgtcatcctt
     7621 caatactttc taaatctttt aacttaaccg aaacaatttt tgagaccccc gattcttgca
     7681 tggtcactcc atagatagaa tcagccgctg ccatggttcc cttacgatgg gttacgacga
     7741 tgaactggct gtccttgtca aagcggttga ggtaatcccc aaaacgttta acattggctt
     7801 cgtccagcgc agcctctacc tcatccaaga tgacaaatgg gatagtctta actcgaatga
     7861 tagaaaaaag caaagcaaga gctgataagg ctttttcacc accactcatg aggttaagcg
     7921 actggatttt cttacctgga ggttgaacag aaatctcaac cccagctgtc aacaagtctc
     7981 cctcagtcaa gattaagtct gcctgacctc ctccaaacat ctgtttgaag gtcactttaa
     8041 aggattcacg aatggcctca aaggttgatt taaagcgatc tttgacctca tcattcatct
     8101 ctgtaatggt ctcaaggagc agatttttcg ctgacaaaat atcatctcgt tggctattta
     8161 ggaaatccag acgattgtga acttcttcgt actggtcaat agcttctata ttgacaggcc
     8221 ccagtgagcg aatagctttc tctaaatcct gcacctcttg ctctgccaga ttgaggtttt
     8281 ccaattcatg cgccttttct agagcttcag tatagctaat ctgatattgg tctgttaatt
     8341 gactttgtaa atagcgcaag cgctcgctga ccttttcttt cttggcttca gcacgtgttt
     8401 gcttgcgaat ccactcttca ttctgttggc gagcctgatc caaatgacta gcaatatcat
     8461 ccagttgacc ttcaatatca tccaactcaa actgcttgcg aatcaaacct tgttggaggt
     8521 ttgttttctg cattttggct tcttccgcct gttgactgag caattctgta tcaactttct
     8581 caagattgtc aaccttttct tgaagaaggc gctggatttc ctcttgttcg atatccagat
     8641 tatccaattc cttgcctaaa cgctcaatat cagcaacttc ataacgtttt tgcccttgca
     8701 gttctgtctt aagcaaacga gcttgcgcta cctgttcttg caagttctga tagcgttctt
     8761 ggatggcatt tttgttggac ttaatctctt caatctcagc ttccagattt tgcttgtctc
     8821 tggcgattgt agcaaggcgc tcttggcagt tttccttatc cgcttgccaa tctccctcgg
     8881 aaagacgatc tatttcctct tcttggagtt tccaaagagt ttccagttct tcaacttgct
     8941 gactagtttg ctgataagcg aggaacaagc cttgctcctg aatacgtgcc tgctctcctt
     9001 gaaatttaat agcttctaat gactcggtca atctggccat ctcatcttgc aaggtcttca
     9061 aagtcgcctc ttctgaaccc aagcttgctt cttcttcagc aatttctttt tgtaattgct
     9121 ccagttctgg cttgataaaa atgctgttat tctggcgatt ggcaccacct gcataagaac
     9181 cacctgtgcg caactctgtc ccatccaatg tcaccatacg aacctgataa cgaacttggc
     9241 gagctgctgc acgcgcatgt tctacggtat caaagatagc cgtcgtagct agcaagttct
     9301 tgaaaatggc ttccagtcta gtatcaaaag tcaccaactc atctgccatc ccaaggaaac
     9361 ctggacttac agcgatagca tcttgattct ggctagaaat cgtacgagcc ttgattgttg
     9421 tcaacggaag gaaggttgca cgaccggctc tgtttcgttt aaggaaatca atagctttgg
     9481 ttgccgactc ttcatcttct acgatgatat gctggctact tgcccctaag gcaatctcta
     9541 gggcagtttg ataataaaca tcaaaggtca gatgctcact gactgcacca ataatcccac
     9601 ctaggcgatc tttttcttgg agaacactct taacacctgc ataaaagtta ctatgatttc
     9661 tcaggatatt ttccaaactt tgagctctgg cctgcttgtt tttgagatta tccagacggt
     9721 caaagagttg gctttgttga gcttgatagg aagttttctg ctcctcttgc tccttggcaa
     9781 tagcttggta gtcagccaat aatttctgaa cctgctcctt ggcagtttca agctcttcct
     9841 tttgctgact agccttctct ttagctatag ccaattgctc tttcagcttt tctagttgat
     9901 ctgcttgttt ttgagaaagc tgacgactat tttccaactc attctcaata cgggtcaact
     9961 ggtttgagac atccgcttct tcttgtaaaa gagctacaaa gcgttcacgt aagagctcaa
    10021 tcatctgatc aggatcgtct gagaaagcca gcaattcagc ttctaaacga tggagttttt
    10081 gattattttg gactagattt ccctctaaca gagctaaaga gctttcttta tcatactttt
    10141 ctttgctgag tgaatttctc ttatcctcca aagcagccaa acgagcttgt gcctcctgtt
    10201 gattcagggc cacttgctcg gactccagtt tcgatagggc taattttctt tctaaatcac
    10261 taatcagact agtcaagtcc atcaaactgc cttggtcttt ggccatttca gcctgtaaat
    10321 cttggcgttg ctttttaaga gtttgatttt cttcttctaa tttttcacgc ttttggtaat
    10381 aactcatcaa gagttcttga acctgagcca gctcttcttc tgtcgactct agttctgcct
    10441 tattttcctt gatttgagca accagaacgt ctaaataaat agccttacgt tgtccttcca
    10501 agtctaaaaa cttacgggca ttctcagctt gcttctcaag aggcttgatt tgattatcca
    10561 actcgtagat aatgtcctct aagcggtcta gattatcctg agtttgttgc agtttactct
    10621 cagtttcttt tctgcgagtc ttgtatttta aaactcctgc agcttcttca aaaatagctc
    10681 ggcgttcctc aggcttagaa ttaaaaatct cctcaacctt cccttgggaa atgatagaga
    10741 aggaatctcg tcccaaacct gtatccaaga aaaggtcatg aatatcacgc agacggactt
    10801 tcttgccgtc aatcttgtat tcgctatctc cactacgata gatatggcgt tctaccctga
    10861 tttcttggcc tgcatccttg ataaatccgt catgattatc cagagtcaca actacagaag
    10921 cataattgag cggtttgcga ctttcggtcc cagcaaagat gacatccggc atcttgcccc
    10981 cacggagact cttgacactt gactccccca aggcccaacg cagactttct gtaatattgg
    11041 actttccaga tccattgggt ccaacaactg ccgtcacacc ttggtcaaaa acgaccttgg
    11101 tcttatcagc aaaagacttg aacccctgaa tttcgatttc ctttaaatac atgaatccag
    11161 ccctttctca acggcatttt tggcagcttc ctgctctgct aatttcttag aacgaccttg
    11221 gccttgaccg atgctcttac cttcaacaag aacttctaca tcaaagacct tatcgtgagc
    11281 aggccctgtt tcagaaatca cctgataacg aatagccaca tcaccattga cctgaagtaa
    11341 ctcttggaga tgggttttgt agtctgtaat catctcaaac tcgcctgctt ctaccttagg
    11401 aatcataact tggtagataa attccttgac cttggccaca tccttatcca aaagaagggc
    11461 accaagaaag gcttcaaagg catcaccaag aatggtgtca cgattgcgac caccagattt
    11521 ttcttcccct ttacccaact tgataaactg gtcaaactgg caatcacgcg caaaaccagc
    11581 taaactctcc tcacggacaa tcatagcacg gagttttgat aggtcacctt caggcttttt
    11641 aggatatttt ttatatagat attctgaaat caataactgt agaacagcgt ctcctaaaaa
    11701 ttccaagcgt tcattgtgtg aaatttttaa gaggcggtgc tcattggcat aactcgtatg
    11761 agtaaaggca gtttccagta aatttttgtc tgcaaattcg attgcaaaat gattctttag
    11821 tacagtttgt aattctttca tactaacctc tttctaactg ataacagtcc tttttattat
    11881 atcaaaaaaa gccccctgag tcactttaaa acgggactgg aggggatttg ga
//
LOCUS       DAVKDZ010000062        11894 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000062 DAVKDZ010000000
VERSION     DAVKDZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11894
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.063"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(50..1252)
                     /gene="rpsA"
                     /locus_tag="VWZ30_001619"
     CDS             complement(50..1252)
                     /gene="rpsA"
                     /locus_tag="VWZ30_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU9335319.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1414..1485)
                     /locus_tag="VWZ30_001620"
     tRNA            complement(1414..1485)
                     /locus_tag="VWZ30_001620"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1451..1453),aa:Gln,seq:ttg)
     gene            complement(1495..1575)
                     /locus_tag="VWZ30_001621"
     tRNA            complement(1495..1575)
                     /locus_tag="VWZ30_001621"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1539..1541),aa:Tyr,seq:gta)
     gene            complement(1625..1855)
                     /locus_tag="VWZ30_001622"
     CDS             complement(1625..1855)
                     /locus_tag="VWZ30_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEU9335320.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(2023..2667)
                     /gene="pcp"
                     /locus_tag="VWZ30_001623"
     CDS             complement(2023..2667)
                     /gene="pcp"
                     /locus_tag="VWZ30_001623"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9335321.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDVVLCIGQAGGRIGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYLSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(2682..3605)
                     /locus_tag="VWZ30_001624"
     CDS             complement(2682..3605)
                     /locus_tag="VWZ30_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEU9335322.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(3602..4291)
                     /locus_tag="VWZ30_001625"
     CDS             complement(3602..4291)
                     /locus_tag="VWZ30_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEU9335323.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(4434..5033)
                     /locus_tag="VWZ30_001626"
     CDS             complement(4434..5033)
                     /locus_tag="VWZ30_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9335324.1"
                     /translation="MACGKTDKEADAPTTFSYVYAVDPASLDYSIATRTSTTDVIGNV
                     IDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQEPASCLNIMDPKSGSAMKHLGITKGK
                     DKDVVAKSGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMPTASFGGSPV
                     VSNVVPFSKPYSQVGIKGDPYIFKGMKLQKDIVTTKEYN"
     gene            complement(5116..6138)
                     /locus_tag="VWZ30_001627"
     CDS             complement(5116..6138)
                     /locus_tag="VWZ30_001627"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU9335325.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAQFKNGQWNQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVIRANEEYVPPYGTGGTLYLRPLLIGIGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTDFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPVEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTCKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(6273..8744)
                     /gene="parC"
                     /locus_tag="VWZ30_001628"
     CDS             complement(6273..8744)
                     /gene="parC"
                     /locus_tag="VWZ30_001628"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU9335326.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDLFSTEVEENDQTLLVQSNKGTIYENRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLKEVFTEAK"
     gene            complement(9164..11107)
                     /gene="parE"
                     /locus_tag="VWZ30_001629"
     CDS             complement(9164..11107)
                     /gene="parE"
                     /locus_tag="VWZ30_001629"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU9335327.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLVAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRREWIEDNVKFTLEEATVF"
     gene            11243..11884
                     /gene="plsY"
                     /locus_tag="VWZ30_001630"
     CDS             11243..11884
                     /gene="plsY"
                     /locus_tag="VWZ30_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU9335328.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
BASE COUNT         3417 a         2693 c         2092 g         3692 t
ORIGIN      
        1 aaaaatccct tgtttagtaa acaaaggatt ttgtgaattt tcaatttgat taaagttcga
       61 tatcaccaaa caaatcagcc attgaaaatc ctgtttgtgt ttctggaagt tcgaaatcac
      121 gcttttcttg acgtcttgga cgacgtggac gagcagcacg tttttcttct ttttgtcctt
      181 cttcttgggc tggacgttct tcaagagctt taatagaaag tgacacgcgt tctgcatctg
      241 cgttaacttc aagaacttta acttgaactt cttgaccaac tttaagagct tcttttggat
      301 tttcaatccg tttgtgtgaa atttgtgata cgtgaacaag tccatcgata cctggcaata
      361 cttcaacaaa tgcaccgaag tcagtcaaac gtttaactgt tccttctact acatcacctt
      421 tagccaattt ttgctcaacg ccatcccatg gtcctggtac tgttgcttta agtgaaagtg
      481 atacacgtcc ttcttcttcg ttaagatcaa ggattttcac ttcaatttct tcaccaacag
      541 ttacaactga ttttggtgat acgttacgtt catgtgacaa ttcagtcaag tgaaccaatc
      601 cgtcaacacc accaaggtcg atgaaagcgc cgaagcttgt gatacgagca actttaccag
      661 ttacaacatc accaacagcc aatttaccga atacttcagc gcgagctgct gcagtagctg
      721 cttcaacaac ttcacgacgt gaaaggatga agcggttttc tttagcgtta acttctttga
      781 ttttagtatc aaattcttga cctacaaaac gctcagcgtt acgtacgaaa cgagtatcca
      841 acattgaagc tgggataaat ccacgaacac cttcaaattc tactgaaagt ccacctttaa
      901 cggcacgcgt tcctttaaca gtaacaactt cttcttcgcg accaacaagt ttgtcccatg
      961 ctttgcgagc ttcaaggcgt tttttagata caaggtatgt aactgtatca gtatctttac
     1021 caactacttg acgaagtaca agaacatcca atacttctcc tactttaaca aagtcattga
     1081 tatctgcatc acgatcgttt gtcaattcgc gaagagtcaa gacaccttca acaccagttc
     1141 cagagattgc aacgttagct tgagtcgcat caactgtcaa tacttcagca ctaacaacat
     1201 caccagtctc aacttgacta acgctattta gcaaatcttc aaattcgttc atctaaaaaa
     1261 tcctccaaca atcaagtttt tcttaaactt gacatactta taattttttt cctaagcacc
     1321 cgcaaggaca tgtttatatc attcacgtct ttcaattata aaaataaaat accctaagat
     1381 attttgcttt ttatcttgat tattacctaa gaactggggt agctggattc gaaccaacgc
     1441 atgagggagt caaagtccct tgccttaccg cttggctata ccccattaat gaaatggaga
     1501 gagagggatt cgaacccccg aacccgaagg agcggattta cagtccgccg cgtttagcct
     1561 cttcgctatc tctcctacaa tcaacatgga ctattatatc atgaaaattt caaaaaaaca
     1621 agacttattt tgctaaatta taattttcaa tcaaaatttc cacagctttt tccaattttt
     1681 tataaaaact atcgacattc ccattcttta taatggcaaa ttgtccgtct aattcggcaa
     1741 tttctccgat aactttttta ccgatagtca atgtataacc ttcaaaactg tctttaccaa
     1801 cattaacttt ggcatccgct acttgaattt cgattttttt atctttcttg ctcatttcat
     1861 acctctcttc actttttcta ttttacaaga aaatcctaaa actagcaaga aaaaactcta
     1921 tatcaggctt ctctgatata gagttttatg taaacaacct caatcgattt agcatagtta
     1981 atagttagta aacaagcata attgactatc ctttttaggt tatcagtgag tctcaccgcc
     2041 tactaactgc aaatccttat ctttgtagtc tacaatagct tcaatagcta cctctatgcc
     2101 tcttacaata tcacatagac tcattcctgc agtattcggc ttatttacta cttgttccat
     2161 catatatgga atatgcatga aacctgctct catattaggg aattttttat ccactaaata
     2221 aagagcttgg tacatcaaat gattgcaaac aaaggttcct gcactattgg atacaactgc
     2281 cggaagtccc tgttttttga tagcttgtac catcgctttg ataggtaaac tacttaaata
     2341 ggccgatgct ccatcaatac gaatcggtgt atcaattggt tgattgcctt cgttatcagg
     2401 tatgcgagca tcatcttgat taatagccac tcgttcaggt gttaagccga tcctgccgcc
     2461 tgcttgtcca atacaaagta caacatctgg ttgatatcgt aatatttctg cctctaaaac
     2521 ttctgacgac ttataaaaaa ccgttggaat ttctacccag cgaacttcag ccccattaat
     2581 ctcagatggt aataatttta cagcctccaa agctggatta atcttttcac ctccaaaagg
     2641 attaaaacct gtaaccaata ttttcatttt attttccttt actaaaatgc gagaaagtac
     2701 attaagaata tgtgaataac aatcattact agagcaacac ctgcttgagc ctttataacg
     2761 ccattctgat ctttcatatc catcaatgct gctggtagag cgttaaaatt agcagccatt
     2821 ggggtcaata aggtcccaca ataacctgct gtcatggcaa gagcaccagc cacaattgga
     2881 ttagctccca gagcaaatac aaagggaact ccaacacctg ctgtaataac ggtgaatgct
     2941 gcaaaagcat ttcccataat cattgtgaat agaaccattc caagaacata ggccaaaact
     3001 cctataaagc gactatctga aggaacaata ccgctaatca gatgagagat aacatcacca
     3061 acacctgcta cagtaaaaat agcccccaaa gcccctaata attgaggaac aatcccactt
     3121 gttgaaactt gctgagtcat tcgattattt tctgataaca gactcttagg gtgactattg
     3181 gtaatcacaa gaacagaaat tgtagcaaac aaggcggcaa ggctaatcga aatcttgcta
     3241 aattctggaa tcatttgcgc taagaccaac gcaagtattg ccatcagcat aactggaata
     3301 aaaattttat ttttcaacct gttagattca atattggctt tcatttcatc taaggatggc
     3361 aaggttccga tacggacttg cttaaacaat gttaacagcg ataataggat tacaataata
     3421 ccaatactca tatttggcat ataggaacca cctataaacg taatagacaa tagagtccaa
     3481 aatgcagatg tcccaagtcg aactgggttt gttttatctt tataactaca ataggctgta
     3541 tggagaaatt gacaaccaat cacaatatag gtcaactcta atagttgctt tgccaactct
     3601 gtcatttttg ttctcctccc ctagtctttt ttgatatcaa ttttttatca aataaataat
     3661 tgtaaatccc tactacaata agtgttataa cagcaacaat aatagatgta gaagcaatcc
     3721 ctgcataatt gctttcatag cctaactgat ctaatgttcc ccctatcaag aggactcccc
     3781 cagcacctac aaacgtattt tgagcaaaga aatttccaaa attttcattc gcagccgcac
     3841 gcgcttttat tgtctcatct tcaacctctg ttaactttct acctaattga gactctgcag
     3901 ctgcttctcc cataggttga accaaaggtc tgacaaactg agggtgtcct cctagacgaa
     3961 ttgaaaagaa accagctaac tctcgaataa agaaataaac tgtatagaag tttccaactg
     4021 tcagaccttt aatctttcga atcaaatcga ttgatcgttg cttgagtcca aaggtttctg
     4081 acagccccac aagaggcaag gtaaccataa aaatcgtgag cactcgctga ttgctaaatt
     4141 cttttcccaa aatctccaaa aattcaacga gagaaacacc tgaaactaaa gctgtaacca
     4201 aaccagctaa gactactgtt gcaattgtat caaattttaa aataaaaccc acaacaataa
     4261 ttgctattcc tattaatcta atccactcca tatcaaactc ctttatattc aaaatgacag
     4321 tatttttaaa attttatcaa gatcaatacc attccttatt taatgtgttt ttctagttct
     4381 ttttggtatt tgctattgga ttccaatttt tctttttgcc attttttaaa aacctagtta
     4441 tattcttttg ttgtaacaat atctttttgc aatttcattc ctttaaagat atatggatcc
     4501 cccttaatac caacttgtga gtatggtttt gagaatggta ctacgttact tacaactgga
     4561 gaaccaccaa atgaagctgt tggcatcaat aatgaactat ctgtcgacca agcttgagct
     4621 ttggcatatt tttcatatct cttctctagg tcagtagtct cagaaacagc atcttctaac
     4681 aatttcttat atttatccaa accagattta gctacaacat ccttatcttt tcctttcgta
     4741 ataccaaggt gtttcatggc agaaccagat tttggatcca taatattcaa gcaagacgct
     4801 ggttcttgat agcttggagc ccatcctgta ctgttcaaat catagtcttt ttgagaagga
     4861 gcaacattgc cgtatttatc attttccatc aagccatcaa taacatttcc aataacgtct
     4921 gtcgtcgatg ttcgagtcgc tatactgtag tccaatgatg ctggatctac tgcatagaca
     4981 taagaaaatg ttgtcggtgc atctgcttct ttatcagttt ttccacaagc cactaaaata
     5041 gctgtcgtgc tcaggaccgc tcctgctgtt aagagtcact ttttcgattt cataaaaaat
     5101 ctattttggt ttattttaat ctacttttac aatccaacct tctggcgctt caatatcgcc
     5161 aaactgaata cccgtcaatt cattatataa tttacaagtc acaggaccta cttctgtttc
     5221 actatagaat acatggaaat catcaccgtg ttgaatacct ccaattggag aaataaccgc
     5281 tgctgtacca caggcacctg cctctacaaa acggtcaaga ttatcaattg gaacatcacc
     5341 ctcaacagga gttaatccca agcgatgttc tgccaaataa agcaaggaat acttggtaat
     5401 agatggcaag atagatggac tcaatggtgt tacaaattca ttatcagctg taattccaaa
     5461 gaagttagct gatccgactt cttcaatctt tgtatgagtt gatgggtcca gatagataac
     5521 atctgagaaa tgacgtgact tggccatttt tcctggtaag agacttgcag catagtttcc
     5581 accaacctta gccgcacctg taccatttgg tgctgcacgg tcgtattcat cctgaatcaa
     5641 gaagtcggtt gggaccaaac cacccttaaa gtaatttcca actggcatag caaagatagt
     5701 aaaaatatac tcctctgctg gttttacccc gataatatct ccgataccaa tcaaaagagg
     5761 gcgaagatag agggttccac ctgttccgta tggtggtacg tattcttcat tcgcacgaat
     5821 aactgcttta caagcttcta caaacatgtc tgtcggaact tgtggcatca agagacggtc
     5881 acatgtacgt tgcaggcgtt tggcattttc gtcaggacgg aacagttgaa cactgccatc
     5941 cttagtacga taagctttca aaccttcaaa tgcttgttga ccatagtgaa gacttggaga
     6001 agactctgaa atatgcaaag ttgcatcctc tgtaagttct ccttgattcc attgtccatt
     6061 tttaaactga gcaagatagc gataaggtaa tttcatatag gaaaaaccga ggttttccca
     6121 atcaatcgtt actgtcatat tccactcctt attctaaaaa cctatttctt atattctaca
     6181 ctatttttct aaaatagcaa gtctgttttg taattttcag aaaatttctc caataacaag
     6241 aaccagctct tagaactgat tcttcatttc acttatttag cttcagtaaa tacttcttta
     6301 agataagcgt caaaaacttc ttcatctgaa atcgtgtcag aaatgaagct tccgttgcta
     6361 gtcctttctg acaggttcaa gtcttgcaat ctgttttcat agattgttcc tttgttggat
     6421 tgaacaagca gcgtttggtc gttctcttct acttctgtac tgaagagatc gccaacaaat
     6481 ccttgctctg caactgctcc tgccaagaag acacgatgcg gtttgttttt caactcacgc
     6541 aagacttgta atcctcgttt ggcacggctg gttgctagaa tttcctcaat ggaaacacgt
     6601 ttcaagcttc cacgctgggt caagaggtag aaggacgaag tattacagat aaagccagat
     6661 tggaggacat catcttcttt caaattcata gccttgacac ctgctgcctt agcaccgaca
     6721 accggaacct cttcgatatt gaaacgcagg gcataaccat tttgactaac caagacaaca
     6781 tcatctagtt taatcggagc cactgctaca atctgatctg tatcgtcttt gagcttagca
     6841 tacttgacag acttagatct ataggtccgc catggagtga attcttttcg ctctacccgt
     6901 ttgatttgac caaggcgagt cactgcaaag taggttgtcg catcgtcaaa ctgatccagt
     6961 acttccacat aaaggatttc ttcattcgtt tcaaagtttg tgatggtttg gctcagatgc
     7021 tctccgatgt ccttccaacg aatatctgcc aactcatgga ttggtctgta gatgacattt
     7081 ccaagacttg tgaacatcaa gaggtgctgg gttgtcttgg cagattgaac aaaaatcaaa
     7141 cggtcatcat cacgcttgcc aatttcttcc aaggtggaag ccgcaaagga acgtggactg
     7201 gtacgcttga tgtaacctgc cttggtcacg ctgacgtagg tatcttcctc agcgataaga
     7261 ctagctgtat caatctcaat tgctttcgca gtgtcttcta aagaactcaa acgaggagtt
     7321 gcaaatttct tcttgacctc acgaagttct ttcttcatga gattgtacat agtcctttca
     7381 tcaccgataa tagccgccag catagcaatc ttctcacgaa gctctgcttc ttcttcctgc
     7441 aagacaacca catcggtatt ggtcaaacgg tacagttgca aagttacgat agcctcagcc
     7501 tgttcttccg taaaatcata gctaactttg aggttttcct tggcgtccgc cttattctca
     7561 gaagcacgga taagagcaat gacttcatcc aaaatcgaaa tcacacgaat caaaccttcg
     7621 acgatatgga gacgtttctc agccttttct ttgtcaaagc gtgaacgcgc caaaatcact
     7681 tctcgacggt gagcgatgta gctagacagg attggaacaa tcccaacctg acgaggtgtg
     7741 aaattgtcaa tcgccaccat attaaagttg tagttgattt gtaggtcggt gtacttaaat
     7801 aagtaattga gaacaagctc agtattagcg tctttcttaa gttcaatagc gatacgaaga
     7861 ccatcacggt cagactcatc acgaacctca gcaatcccag ctaccttgtt attaacacga
     7921 acatcatcga ttttcttgac tagattggcc ttattgattt cataaggaat ctcagtaata
     7981 acgatttgtt ccttaccacc ttttagcttt tcaatttcag tcttggaacg aacaaccacg
     8041 cgccctttcc cagtctcata agctttcttg atttcatcac gaccctgaat aatagcccct
     8101 gtagggaagt ctggtccagg taagaattcc atgagtttat caatctttgc agttgggtgg
     8161 tcaatcatgt aaactgcagc atctatgacc tcagctaaat tatggggagg aatgtctgtg
     8221 gcataaccag ccgaaatccc agtcgaacca ttgaccaaga ggtttggaaa ggctgctggc
     8281 aagaccgttg gttctttctc cgtatcgtca aagttccatg caaaaggaac tgtctttttc
     8341 tcgatatcct gaagaaggta gcctgcaatt tcagacaaac gtgcctcagt ataacgcata
     8401 gctgcaggag gatctccgtc catagaaccg ttattaccgt gcatttcaac tagaatctca
     8461 cgatttttcc agttctgtga catacgaacc atggcatcat agatagaaga atccccgtgt
     8521 gggtggaaat tccccatgat gttcccgact gacttagccg acttacggta gctcttgtca
     8581 aaagtattgc tatccttatt catagaataa agaatacggc gctgaaccgg cttcaaccca
     8641 tcacgaatat ctggcaaagc ccggtcttga ataatgtact tggagtagcg accaaagcgc
     8701 tctcccatga tgtcctccag ggacatgttt tgaatgttag acataagata caaagcccat
     8761 aaaataccaa gtaaaaatag aaaattcttg aagtaagcaa actcacaaga gaatttatct
     8821 ttttcacaca gtatctaggg cgtgttcaac tcctttcaaa gaatgtagag taggttttta
     8881 tgcagtaaaa gatattttac gggaattcct cccgtgttca gttacgataa gtaaccaaac
     8941 tatcctgttt gtatttttca atatgaaaat ctggttttcc aaaattagtc ttagtttgtg
     9001 tcttagccgc tcccttaagc gcctctttga gataagcact catagcagat tcttcattaa
     9061 taatcctgca attttttcaa accaagattt tcaaactgct ttttcacata gtcattcaca
     9121 tccgactcta atttccagtt tactaacata ttattttctt tcattaaaac actgtcgctt
     9181 cttctagcgt aaacttgaca ttatcttcaa tccattcacg gcgtggttct accttatctc
     9241 ccatgagaac attgacgcgg cgttcggcgc gcgctaaatc ttcaattgtg acacggatga
     9301 gggtacgtgt ttctgggttc atggttgttt cccagagctg gtccgcattc atctcaccaa
     9361 gtcctttgta tcgttggagg gtagcgcctt taccgaactg tttacggagt tcttctagtt
     9421 ctccgtccgt ccaagcgtag gccacttctt ctttcttgcc tttacctttg gacatcttgt
     9481 aaagaggtgg gagggcaata tagacatgac ctgcctcgac tagcggacgc atgtaacggt
     9541 agaaaaatgt caagagcaag gtctggatat gggcaccgtc ggtatccgca tcggtcatga
     9601 taatgatctt atcatagttg gcatcttcaa tagagaagtc tgctccaaca cccgcaccaa
     9661 tggtataaat catggtattg atctcttcat ttttgaggat atccgccatc ttggccttgg
     9721 ctgtattgac aaccttacca cgaagaggta gaatagcctg gaacttgcgg tcacgacctt
     9781 gtttggcaga accaccggca gagtccccct caactagata gagttcattc ttagcaggat
     9841 tcttagattg ggctggggtc aatttcccag acaacaagcc cttatctttc ttgtttttct
     9901 tcccatttcg gctctcatca cgcgccttac gtgctgcttc acgagcatca cgggccttga
     9961 tagccttgcg gatgaggtta gaagctaatt ccccattttc cataaggaaa aaggtcaact
    10021 tatcagccac tattccatcc acaactgggc gagctagggg gcttcctagt ttatccttgg
    10081 tctgtccttc aaactgcaag tgttcttcag gaactaagat agaaagaacg gccactagtc
    10141 cctcacgata gtctgaacct tcaaggtttt tatctttttc cttaagaaga cctgttttac
    10201 gtgcatagtc attcatgacc ttggtaatgg cagacttgag tcctgtctcg tgcgttccac
    10261 cgtccttggt gcgaacgtta ttgacaaagg atagaatgtt atctgagaat ccgtcattgt
    10321 actggagggc tacttccact tgaaaaccat tgtcttcccc ttcaaagtaa agaactggcg
    10381 tcaagatttc cttatcttcg ttgagataag aaacaaaatc ttgtactcca ttctcatagt
    10441 ggaactcaat cgcttcatct gttcgcttgt ccgttaaaga caaggtcaca tttttcaaga
    10501 gaaaggctga ttcattaagg cgctctgaaa tggtattgta cttgaaatct gtcgtagaaa
    10561 agatagtcgc gtcaggcata aaagtaactt tggtgcctgt tttagacttg ggtgctgtac
    10621 cgattttctt caaagtcgtg acaggttttc caccattttc gaaacgttgc ttgtaaactg
    10681 cgccatcacg ggtaatttca acttctaacc agctagaaag ggcgttaaca acggaagaac
    10741 ccactccgtg aagtccacct gatgtcttat agccaccttg accgaatttc cctccggcat
    10801 gaagaatggt aaagataacc tcaacagttg gaattcccat agcgtgcata cctgtcggca
    10861 tcccacgtcc atggtcttga accgttagac taccgtcttt attgatagtt acatcgatac
    10921 gatcaccaaa cccagacaag gcttcatcga ctgcattatc aacgatttcc caaactaggt
    10981 gatgaagacc agcgccatcg gtcgatccaa tatacatccc tggacgtttt cggaccgcat
    11041 ccaacccttc tagcacctga atagcatcat cattataatt gttaatattg atttcctttt
    11101 ttgacacaag gaacctccta ttcgttcatc tttactattc tacaggtttt ccaaggattt
    11161 tgcaaaattt ttctttctcc gatgtgacaa tttcagcaga gattctctgc ttttctttcc
    11221 caattcatga tataatagga gtatgattac aatagtttta ttaatcctag cctatctgct
    11281 gggttcgatt ccatctggtc tctggattgg acaagtattc tttcaaatca atctacgcga
    11341 gcatggttct ggtaacactg gaacgaccaa taccttccgc attttaggta agaaagctgg
    11401 tatggcaacc tttgtgattg actttttcaa aggaacccta gcaacgctgc ttccgattat
    11461 ttttcatcta caaggcgttt ctcctctcat ctttggactt ttggctgtta tcggccatac
    11521 cttccctatc tttgcaggat ttaaaggtgg taaggctgtc gcaaccagtg ctggagtgat
    11581 tttcggattt gcgcctatct tctgtctcta ccttgcgatt atcttctttg gagctctcta
    11641 tcttggcagt atgatttcac tgtctagtgt cacagcatcg atcgcggctg ttatcggggt
    11701 tctgctcttt ccactttttg gttttatcct gagtaactat gactctctct tcatcgctat
    11761 tatcttagca cttgctagtt tgattatcat tcgtcataag gacaatatag ctcgtatcaa
    11821 aaataaaact gaaaatttgg tcccttgggg attgaaccta actcatcaag atcctaaaaa
    11881 ataaaacgcc agtt
//
LOCUS       DAVKDZ010000063        11873 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000063 DAVKDZ010000000
VERSION     DAVKDZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11873
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.064"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(135..1943)
                     /gene="glmS"
                     /locus_tag="VWZ30_001631"
     CDS             complement(135..1943)
                     /gene="glmS"
                     /locus_tag="VWZ30_001631"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU9335329.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDSNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEVGQ
                     VVVDQAIIQAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANHTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEVKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLTLLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKETDAIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(2136..3515)
                     /gene="bglA"
                     /locus_tag="VWZ30_001632"
     CDS             complement(2136..3515)
                     /gene="bglA"
                     /locus_tag="VWZ30_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU9335330.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVLAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(3614..5467)
                     /locus_tag="VWZ30_001633"
     CDS             complement(3614..5467)
                     /locus_tag="VWZ30_001633"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU9335331.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(5480..6739)
                     /gene="rseP"
                     /locus_tag="VWZ30_001634"
     CDS             complement(5480..6739)
                     /gene="rseP"
                     /locus_tag="VWZ30_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU9335332.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(6761..7564)
                     /locus_tag="VWZ30_001635"
     CDS             complement(6761..7564)
                     /locus_tag="VWZ30_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU9335333.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(7573..8331)
                     /locus_tag="VWZ30_001636"
     CDS             complement(7573..8331)
                     /locus_tag="VWZ30_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU9335334.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEVALQEAILAYNRRHRRFGGV"
     gene            complement(8562..9134)
                     /locus_tag="VWZ30_001637"
     CDS             complement(8562..9134)
                     /locus_tag="VWZ30_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU9335335.1"
                     /translation="MSTMKNKQEILESFRKNPDMMAILTIIQNLELTDSWLAAGSVRN
                     FIWNLLSDKSPFDFETDVDVIFFDPDISYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTAPYTSSRDAMSKYPERCTAVGLRLNEEPALELFTPYGLEDILNFQVRPTPHFL
                     ENQERMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(9115..10086)
                     /gene="ruvB"
                     /locus_tag="VWZ30_001638"
     CDS             complement(9115..10086)
                     /gene="ruvB"
                     /locus_tag="VWZ30_001638"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU9335336.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNDLEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDVITDKALTMLDVDHEGLDYVDQKILRTMIEMYGGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRMATAKAYEHLGYEYNEK"
     gene            10246..10338
                     /locus_tag="VWZ30_001639"
     CDS             10246..10338
                     /locus_tag="VWZ30_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU9335337.1"
                     /translation="MMELVLKTIIGPFVVGVVLRLVDKWLNKDK"
     gene            10653..10745
                     /locus_tag="VWZ30_001640"
     CDS             10653..10745
                     /locus_tag="VWZ30_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU9335338.1"
                     /translation="MMDTILKTIIGPIVVGVVLRLVDKWLNKDK"
     gene            11086..11178
                     /locus_tag="VWZ30_001641"
     CDS             11086..11178
                     /locus_tag="VWZ30_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU9335339.1"
                     /translation="MMELILKTIIGPIVVGVVLRLVDKWLNKDR"
BASE COUNT         3535 a         2744 c         2207 g         3387 t
ORIGIN      
        1 cctgtgtact gttctaaatt caatctgcta taattattga aaagtaaacc cttatcatca
       61 tttgtaacat aaaaaatagg ctgtaaaacc ttataggata aacctatggc tttacgcccg
      121 tatattcata gattctattc taccgttact gacttggcaa ggttacgtgg tttgtccaca
      181 tcgaggccac ggtggagggt tgcaaagtaa gcgaccaatt gtgttggtac gaccattgaa
      241 attggtgaga ggtaagggtg tacagtcgta aggacgatag cgtctgtctc tttagcaaca
      301 ttttcttctg cgatagtgag gacttttgca ccacgggctg ccacctcttg gatatttcca
      361 cgagtgtggt tggcaaggac tggatctgac aagagagtca aaacaggtgt tccttcttca
      421 atcaaggcaa tagttccgtg cttaagttct cctgccgcaa agccttcaca ctggatgtaa
      481 gaaatctctt taagtttgag acttgcttcc atggctacgt agtaatcttg accacgtccg
      541 atgtaaaagg cgttacgagt tgtttcaagt agctcacgaa ccttgacttc aatggtttct
      601 ttctctgaaa gagttgattc gatagactga gctacgattg acaactcatg aactaaatca
      661 aaggcttgcg ctttggcatt gccatttgct tctccgactg cttttgcaag gaaggcaagg
      721 gctgcaattt gcgctgtata ggccttggtt gatgccacgg caatttcagg acctgcatga
      781 aggagcatgg tatggttggc ttcacgagaa agagttgaac ctgggacgtt tgtcactgtc
      841 aagcttggga ttcccatttc attagccttg accaaaacct gacggctatc tgctgtttca
      901 ccagactggc tgataaagat gaagagtggt ttcttgctga gaagtggcat accgtaaccc
      961 cactcagatg aaattccaag ttcaactggt gtatctgtca attcttccag catcttctta
     1021 gaagcaaatc ctgcgtggta agatgtccca gctgcaagga tgtagatgcg gtctgcgtct
     1081 tgaacagcct gaatgatagc ttggtctacg actacttgac caacctcatc tgtgtaggct
     1141 tggatcaact tacgcatcac tgttggttgc tcgtcaattt ccttgagcat gtagtaaggg
     1201 taagttccct taccgatatc tgacaagtca agttccgcag tatagctagc acgttcacga
     1261 ctgttactat catagtcttg aacttccacg ctatcagcct tgacgattac caactcttgg
     1321 tcatgaattt ccatgtattg gttggtttca cgaatcatag ccatagcatc tgagcagacc
     1381 atattgtagc cttccccaag accaatcaaa agtggagatt tgtttttcgc tacatagatg
     1441 acatctggat tttcagagtc aatcaaggca aaggcatatg aaccacggat aatatgaaga
     1501 gcttttttaa aggcttcaag aactgagagc ccttcttctt ccgcaaattt tccaatcaaa
     1561 tgaacggcta tttcagtatc tgtctgcccc ttgaagtggt gacctgcaag gtattcttcc
     1621 ttgatttcaa gatagttctc aatcacccca ttatgcacca agacaaaacg ttctgtctca
     1681 gagcggtgtg ggtgagcatt gtcctcagtt ggttttccgt gagtagccca acgagtatgt
     1741 ccgataccag ttgttccctc aacaccagct gtcttggcag acaattctgc aatacgacca
     1801 accgccttca ccaaatggtt atcagcacca tctaggacaa aaattcccgc agaatcatag
     1861 ccacggtatt caagcttttc aagcccttga atcaaaatat cagttgcatt tgtgtttcca
     1921 acaacaccaa caattccaca catagtatat acgacacagg caagctgtgc tttctcctta
     1981 aaattggtat agtctaattc atcttttata gaatcagcaa aaacagtata tacttgtttc
     2041 tttcacttgt caagagtaaa aattggtata gttcaaatta agctcctgta agcataaaaa
     2101 ctctgaccga ttgggataat cagtcagagt cctttttaaa atccattatt atcgcttaat
     2161 tctttgaacc agtggcctga tttcttcaga cgacgttctt gcgtttccaa gtctaattcg
     2221 accaaaccat agcgattttt atagctgttg agccatgacc agcagtcaat aaaggtccaa
     2281 atcaagtagc ccttacagtt ggcaccatct tcaatggcac ggtgaagttc acgaagatga
     2341 ccttttacaa agtcaatacg gtaatcatct tgaatcattc catcttgacg gaatttttct
     2401 tccccttcaa cacccatacc attctcagtc aacatccact caatattgcc ataattttcc
     2461 ttgatatttt gggcgatgtc ataaatccct tgctcataaa tctcccaacc acgatgagaa
     2521 ttgattttac gtccaggcat cacataaggc tcgtaaaaat gttctggtaa gagtggactc
     2581 tctggatgct tagcaaatcg aggagccata acacgcaaag gttgatagta gttcacacca
     2641 aggaagtcca ccgtattatc acgaatgagt tccaactctt cctctgtagc atcaggtaaa
     2701 agaccgtgtt catgcaagat ttctaccaac tcctgtggat aagtccccaa gacagatgga
     2761 tctaagaaag attgggcctg aaaaagggcc gcaatacgag ctgccttgac atcagcagga
     2821 tgctggctac gtggataagc cggtgtcaag ttgaggacaa tcccaatctt ggaatcaggc
     2881 aaaagttcat ggcaagcctt aacagcccga ctgctggcca attgtgtatg ataggctacc
     2941 ttaacagctg cctctgcatc caccttatgt ggataatggg catcataaaa ataaccaaat
     3001 tctacaggaa cgatgggctc gttaaaggta atccattgat ccactaaatc tccataagtc
     3061 tcaaaacaaa aacgagcata gtcttcatag gctaagactg ttgccttatt ttcccaacca
     3121 tcaccatcct cttgaagggc aaaaggtaaa tcaaaatgat agagattgac taacagacga
     3181 attcctttag ccttaatagc ctcaaagacc ttacgataaa aatccacacc ttgagggttg
     3241 acttttccac agccttgtgg aaaaatccgt gaccactgaa tagaagtccg aaaggctgtg
     3301 tgaccagtct ctaacaaaag ctcaatatcc cgctcccaat tttcataaaa agtcgatgtc
     3361 ttatctgaac caatcccatt atagtaacga tttggctcca cttggaacca gtaatcccag
     3421 agattgtctc ccttaccgtc accagctaca cgtccttctg tctgcggtcc agaagtagag
     3481 gagccccaga caaaatcctt tggaaatctt agcatacatt tacctcttta tctactcatt
     3541 tctcccatta tacagaaaaa acaaggtaaa aactagttac attttttcct tgtttttctt
     3601 ctgattatag tttttatttc ttgcttagga tttcaagcgt ttcaagcacg ttatctgcat
     3661 gaacctcaat ggtgtcacca gtcgccttaa tcttaacttc tacgatgcca tcagctgctt
     3721 tcttcccaac agtgatacgg attggtaacc caatcaaatc gctatcacta aacttcactc
     3781 cgacacgttc gttacggtca tctgtcaaga cttcataacc agctcccatc aagcttgctt
     3841 caagtttttc tgtcaaggct tgcgcttctt catccttgac attgacagta atcaaatgca
     3901 catcaaatgg tgccaattct ttagggaaat tgattcccca agcgtaacgg tattcacctt
     3961 ttggcgtttt gttaacaaag aggcgagcgt gttgctccat cactgctgaa agaagacggc
     4021 tgacaccgat accgtaacat cccatgatga ttggcacagc acgaccattt tcatccaaga
     4081 catctgctcc catgcttgct gaatagcgag ttccgagttt gaaaatatga ccgatctcaa
     4141 taccacgcgc aaagttaagg acaccttgtc catctgggga aatttcaccc tcacgaactt
     4201 cacggatatc cacatattct gcagtaaaat cacggcctgg gttcacaccc gtcaagtggt
     4261 agccatcttc gttagcaccg acaactgcat tgcgaacatc ttgcacctta cgatctgcaa
     4321 taattttaat attctctggc aaaccaactg gtccaagtga accaaatcct gcttgaacaa
     4381 cattcgccac ttcttcttcg ctagcaacgt caaagaaatc tgctcccaag tggtttttca
     4441 acttgacttc gttgagttgg tcatttccaa ctagaagggc tgcaacaagc tcaccatctg
     4501 caatgtagaa gagggtttta atcgtttgtt cttctggaac attgaggaaa gctgcaactt
     4561 catcaattga tttaacatct ggcgttgcaa cgcgagtaac ttcttcttca gcgacaacac
     4621 ggttgcttgg tttgtactcg tttgttgcca tttctaagtt agctgcatag ctagactcac
     4681 ttgagtaagc aatggtatct tcaccagaga ctatccattt gagcaattct gccttgattt
     4741 cttcttgcac ttctgcagga atttcgtcaa atgaggcaac tgacttgtcc aagacaaccc
     4801 agcggtcaag gtctgtacga gcagatgtaa tggccataaa ttcttggcta tccttaccac
     4861 ccatggctcc accgtcacca ataatagcct tgaagtctaa accactacga gtgaaaatac
     4921 gctcataggc tgctttgtac tcatcataaa cactatccaa actatcatag ttagcgtgga
     4981 aactataagc atccttcatg ataaactcac gtgtacgaag aagtccatta cgtgggcgtt
     5041 tttcatcacg atacttgggc tgaatttgat aaaggttgag tggcaattgc ttgtaagatt
     5101 taacagaatc acggacaata gctgtaaagg tttcttcgtg agttggacct aagataaagt
     5161 ctgatttttc acggtttttt agtttgtaaa ggtcttcacc ataggtttcg taacgacctg
     5221 attcacgcca caattctgca ctaagaaggg ctggagccag catctcaaca gcaccaatct
     5281 tttcgaattc ttggcgcatg atgtttttag ctttttcaat cacacgattg gcaagtggta
     5341 gataagaata aacacctgct gaaacttggc gaacataacc agcacgcaac ataagagcat
     5401 ggctgataac ttgagcatcg cttggcattt cgcgaagcgt tgggataggc attttacttt
     5461 gtttcataat attcctcgat tatctaaaaa agagtcgcat aatgtcattc caagtcacag
     5521 caatcatcaa gacaaccatg atgaccactc cggccaaggt gacataggtt tcaatttctt
     5581 gtttcaatgg tttgcggcgg atggcttcta ggatattgag cacaatctta ccaccatcca
     5641 aggctggaat cggaataaga ttaaaaatcc caatattgat ggaaatcatt gccaagaagt
     5701 acaagatatt ttcaattcca tttttagcag catcactact tgccttaaag atagcaacag
     5761 gtccacccaa cttgttcaaa tctggttgga aaatcagatt tttcagagct gagagaattc
     5821 ggagagctga gtcagcagca gttgtaaaac cacctacaaa catggataga aaatctgact
     5881 taacccccgg ttgaacacct agaaggtaac gaccttgact atcttcgggt gtaacagtga
     5941 cttgtttgtc actccccttt tcagaaatag tcacatccaa agtcggtgcc gtcttatctt
     6001 tggtttctgt ttccacagct tggatcaagc tttcccagtt gctaacctca tgtgagccaa
     6061 tcttggtaat ttgtgccgtt tctggtactc ctaccttggc caaggcacct tggggcatga
     6121 tatggaactg attggtatca acatctctga caccaccctg cataaagatt aaaacccaaa
     6181 aaacaacgac acctaagata aaattgttca taggacctgc aaaattggta atcagtttgc
     6241 cccagatagt cgcattttga tattgaacat ctaaaggtgc aatccgaacc tcagtaccat
     6301 ctgcttccac aaccgttgca tcgtgatcca ctgcaaatgt tttttcttct tccagaacca
     6361 atcctttgat aaagagcttg tcttcaaaat caaactgggt cacctgcata gggagggctg
     6421 tttgatccaa ttttttacct gagagattga tgcgtttaac cttaccatca tcagcaagtg
     6481 tcaaactaac aggcgttcct gtcttgattt cagttgtatc atcaccccaa ccggccatgc
     6541 ggacatagcc acccagaggc aagattcgaa tggtataggc cgttccatcc ttgccaatgt
     6601 gagcaaaaat tttaggtccc ataccgatgg caaattcacg tactaaaatc cctgatttct
     6661 tggcaaagta gaagtgcccg aactcgtgca ccactacaat aatcccgaaa accagaataa
     6721 aggttaaaat tccgagcata gcgtttcctc cgtcttttga ttaaaagagt ccaaataagt
     6781 gcatgattgg aaatacaagc aacatactat cgaaacgatc caaaacacca ccatgtccag
     6841 ggataaattt cccagaatcc ttaacaccaa aatgacgttt gatcgaactt tctagtaaat
     6901 caccaaattg tccagcaatg ctaaagaaaa tagcaaagac tgacatcttg taaattccat
     6961 atggaagagc aactgtactg tcaactatca taaagataat ggttactaaa attgctccta
     7021 aaataccacc caaggcaccc tcaagggttt tattaggcga tacccttggt gctaactttc
     7081 gtttcccata gttcatccca acaagatagg caccactgtc tgtcgcccag acgatacaca
     7141 aggctaagag agccttgtcc aaacctgcaa cacgagcatc tagtaaagca ttaaatccaa
     7201 agcccacgta gaagctcata gcaagaggga aaaccgcatc ctcaatcgta taagacttgc
     7261 taaaaacggt cgttcctaac atgattgaaa tcaaaacact ataggcaacc acattcccat
     7321 caactggcaa aaaagtcagg taattctcca agggaatggt caatgcaaag gttgcaaaga
     7381 gggtcaagag gccctccatc gtcatggtct ctagacctct catcttcaaa agttcatgca
     7441 tggctagcat ggctatgatt ccgattgcta tctgaagcaa gaggccccca atcattaaaa
     7501 ttggtaggaa aatagccagg gcaatccctg caaacaaggt tcttttctgt aaatcctggg
     7561 tcatatttcc tcctaaactc ctccaaatcg gcgatgacga cgattatagg caagaatagc
     7621 ttcctgcaag gccacttcgt caaaatcagg ccataaggtg tccgtaaaat aaagctcact
     7681 ataggctccc tgccatggaa ggaaattgct caaacgtaat tctccactag tacggataat
     7741 caagtctggg tctcgtaagt ccttaggcaa atgctgggta aagagatagt tgccaatcaa
     7801 ttcctctgtg atgtcacctg ggttgatttt ggcatctaaa acatcctggg aaatcaactt
     7861 aagcgcctgt gtaatctcag cacgtccacc atagttaaga gcaaaattaa gaatcaatcc
     7921 tgtgttgttc ttagtcaatt cctcagcctt ggttaaagct tcaaaggttt gcttaggcag
     7981 gcggtctgtc tccccaatca tttgaatctt aacattattc gcatgtagtt ccggaacata
     8041 attatcataa aactctactg gcaagttcat gataaacttg acttcctgat ctggacgggt
     8101 ccagttttcc gtagaaaaag catagaccgt aataaccttg acgcccagtt tgttggctgc
     8161 cttggtcacg gtttgcaatg cttccatgcc cgccttatgt ccaaaaactc gcggttgcat
     8221 acgtttttta gcccaacggc cattgccatc catgatgatg ccgatatgag caggaacctg
     8281 tgtcggaacc tctacttcca cagccttatc tttcttaaaa aatccaaaca tgatcttatt
     8341 cctattcaaa aatctatcgt ttcattatac catatttccc cattttcttc tatcactaag
     8401 ctatttattc tcaggcacca agcccatttt tcaaaaaaat aagccgcctg attgggcgac
     8461 tttattttta tagggagatt attatgaaaa agttttagga gtttaagtta aggtcttctt
     8521 aacttatgaa cttagtgtac actccctagc ttaaagtttc cttaagtatt tttaaaaatc
     8581 aaatttttcc atttctcctg ccaatttttc ttggataaac gtgtttgata gagttccatt
     8641 cgttcttgat tctctaaaaa atgaggagtt ggacgaactt gaaaattcaa aatatcctcc
     8701 agaccgtatg gagtaaagag ttccaaagca ggttcttcat tcaagcgcag tccaacagcc
     8761 gtacatcgtt ctggatactt actcatagca tcacgggaac tggtataggg agcagtatga
     8821 gggctgtgct ggtgcatata gacttgattt ttcaattccc actggtactg aggaaagtcc
     8881 tctcgcagtt ttttctccag taataaggtt tcctcataag aaatatccgg atcaaagaaa
     8941 atcacatcta catctgtttc aaagtcaaaa ggtgatttgt ctgacaagag attccagatg
     9001 aaatttctga cagaacctgc tgccaaccag gaatctgtca actccaggtt ttggatgata
     9061 gtcagaatag ccatcatatc tggatttttt ctaaaagact ctagaatttc ttgcttattt
     9121 ttcattgtac tcatacccca aatgctcata tgccttagca gtcgccatcc gtccagaccg
     9181 tgtccgcatg ataaaacctt tttgaatcaa gtaaggctca tacatgtctt ctacagtctc
     9241 acgctcctcg gcaatattga cagaaagagt tcctaatccg acagggccac caccgtacat
     9301 ctcaatcatg gtgcgaagga ttttttgatc cacatagtcc aaaccttcgt ggtcaacatc
     9361 cagcatagtc aaagccttat cggtaataac atcatcgata accccattcc ccattatctg
     9421 ggcaaaatcg cgcacgcgct tgaggagacg attggcaata cgaggggtcc cacgactacg
     9481 tagggccaac tcagatgctg cctcatgagt gatttccatc tcaaaaatat ctgccgtccg
     9541 ctcgacaatt tctgtcaagt cagcatgggc ataatactcc atatggcctg taatcccaaa
     9601 acgtgcccgt agtggatttg agagcatacc agcccgagtc gtcgcaccaa tcaaggtaaa
     9661 aggtggtaac tccaaatgaa cactacgact accctcacca gccccaatca taatatctat
     9721 gtagaagtcc tccatagcac tataaagcac ctcttccact gacattggca agcgatgaat
     9781 ctcatcaata aaaaggacat ccccaggctc taaatcattc aaaattgcta ccagatcacc
     9841 ggctttttca atgacaggcc cagaggtctg ctttagattt acacctagtt cgttggcaat
     9901 aacaaaggcc atggtcgttt ttcccaagcc tggaggccca aataagagca catgatccag
     9961 cgcttcatcc cgcattttgg cagcctcaat aaagatttgt agctggtcct tgaccttgtc
    10021 ctgcccaata tattcacgta agtactgagg acggagcgtg cgttctacta actcctcgtc
    10081 ccctattatc tcattgtcta aaattctact catggctcta ttatatcaaa aatccaagcc
    10141 acaaacaaaa agccacccga ttgggtaact cctgagttta gcacttatgt ggtataatat
    10201 catacggcac tgctacaccg cctacgaaag gaggtgagat agcccatgat ggaattagta
    10261 ctcaaaacta tcatcggacc atttgtggtc ggtgtcgttc ttcgattagt cgataaatgg
    10321 ctaaacaagg acaaatagtg tcaaaaaaag accccaagct tatttggtcg tgagcttggg
    10381 gtcttttcta gtccatgata tagaactagt actcaattcc tttttattat cccatagttc
    10441 acaaattttg tcaaagtttt acattttcat ccccgccaat tccccgacca aaatccgaaa
    10501 aatatcgaaa aatgattttt agaatagtcc ccaaaagcct aaaatagagc caaaaaactc
    10561 cacctgattg ggtgaattcc gactttaaca cttatgtggt ataatattgt tcggcacttc
    10621 tacaccgcct acgaaaggag gtgaattagc ccatgatgga tacaatactt aaaactatca
    10681 tcggaccaat tgtggtcggt gtcgttcttc gattagtcga taaatggcta aacaaggaca
    10741 aatagtgtca aaaagacccc aagcttattt ggtcgtgagc ttggggtctt ttctagccta
    10801 tgatatagac acaatactta attccctttt attatcccat agttcacaaa atttgtcaaa
    10861 ggtttatatc ttcatcaatt cagcttctcc aactttagaa tctgtccaat aaaatttata
    10921 agattaagca aaagttatcg gaaaatgatg tttaaaatag tccccaaaag cctgaaatag
    10981 agcaaaaaaa ctccacctga ttgggtgcct cccgagttta atacttatgt ggtataatat
    11041 tctacggcac tgctacaccg cctacgaaag gaggtgagat agcccatgat ggaactaatc
    11101 cttaaaacta tcatcggacc aattgtggtc ggtgttgttc ttcgcttagt cgataaatgg
    11161 ctaaacaaag atagatagtg tcaaaaaaga ccccgagctt aatgtggaag ttagcttggg
    11221 gtcttttcta gtccatgata tagaactaat ccttaattcc tctccattat cccacagttc
    11281 acaaaatttg tcaaaacttt acatcctcag cttctcaagc tttaaccgct ttacaacaag
    11341 actttcaagt ttaagagaaa attagggatt ctatcaattt catagaaatt ttgatttagt
    11401 aaacgaagag acaatcttat atgtcactcc tcatttaata cgccactact ggacaagcaa
    11461 aatcattatt acagcagttt cagtccttca attaacagtc acttacaatc aaattgagtt
    11521 tgaagtagct gaagtaacaa caaacctatt tcttagtcat attttctaaa aaagttcccg
    11581 ccaattcccc gaccaaaatc cgaaaaatac caaaaaatat cgaaaaatga tttttagaat
    11641 agtccccaaa agcctgaaat aaagccaaaa aactccacct gattgggtgg agttaaggga
    11701 gattattatg aaaaagaaaa gtttaggatt ttattaaata aagttaggag gtctttattt
    11761 aataactata tgatacaaga caaagcttaa aactagctta acttttctca aattctacca
    11821 tttttcaaaa aatttctatt accatcacct caccccacaa aaagtcacct gat
//
LOCUS       DAVKDZ010000064        11558 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000064 DAVKDZ010000000
VERSION     DAVKDZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11558
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.065"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            437..1147
                     /gene="deoD"
                     /locus_tag="VWZ30_001642"
     CDS             437..1147
                     /gene="deoD"
                     /locus_tag="VWZ30_001642"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9335340.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLGDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1170..1694)
                     /locus_tag="VWZ30_001643"
     CDS             complement(1170..1694)
                     /locus_tag="VWZ30_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEU9335341.1"
                     /translation="MKIAIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(1747..1983)
                     /locus_tag="VWZ30_001644"
     CDS             complement(1747..1983)
                     /locus_tag="VWZ30_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU9335342.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(2051..2971)
                     /locus_tag="VWZ30_001645"
     CDS             complement(2051..2971)
                     /locus_tag="VWZ30_001645"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU9335343.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGSLNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWSSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            3161..3564
                     /locus_tag="VWZ30_001646"
                     /pseudo
     CDS             3161..3564
                     /locus_tag="VWZ30_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3780..4361
                     /locus_tag="VWZ30_001647"
     CDS             3780..4361
                     /locus_tag="VWZ30_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9335344.1"
                     /translation="MYYAENPDTAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            4358..5635
                     /locus_tag="VWZ30_001648"
     CDS             4358..5635
                     /locus_tag="VWZ30_001648"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEU9335345.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIRGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTIAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVHQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            5653..6315
                     /gene="deoC"
                     /locus_tag="VWZ30_001649"
     CDS             5653..6315
                     /gene="deoC"
                     /locus_tag="VWZ30_001649"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU9335346.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            6302..6691
                     /locus_tag="VWZ30_001650"
     CDS             6302..6691
                     /locus_tag="VWZ30_001650"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9335347.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAVFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            6781..7833
                     /locus_tag="VWZ30_001651"
     CDS             6781..7833
                     /locus_tag="VWZ30_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEU9335348.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQDWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDIANKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            7977..9512
                     /locus_tag="VWZ30_001652"
     CDS             7977..9512
                     /locus_tag="VWZ30_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9335349.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVVENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEITGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RVASELVPAAALSGGNQQKAIIAREIDRVPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            9505..10563
                     /locus_tag="VWZ30_001653"
     CDS             9505..10563
                     /locus_tag="VWZ30_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9335350.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGLVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLSELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGASLAV
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            10566..11522
                     /locus_tag="VWZ30_001654"
     CDS             10566..11522
                     /locus_tag="VWZ30_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9335351.1"
                     /translation="MSITTLLTLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGCLFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYI
                     AIAFSFLAWFILFKTRFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPTVYLQIAPYVLTILVSAAFFGKAVAPKADGINYIKSK"
BASE COUNT         3289 a         2083 c         2641 g         3545 t
ORIGIN      
        1 tggtttgaag agattttcga agagtataaa cagaaaggta gagtgcgtgt tctaatttga
       61 acacgagtag aaaacttttc taaaaacaaa aacgaaagga tgggtaaact gtattcgcta
      121 aactgaatac gggcgactct cctctaaatc aaaattaaga aagaaaggaa ttgaccccac
      181 cctaaaagca gtgggaaaaa gatagttggt ctagcgagca tcgctcactg cgcccaactc
      241 ctattttccc ttcgcttttt gacgggtttg gtatctttct caatataaaa tataaaataa
      301 agaaaggtag agcgtgtgtt ttgatttgaa cacgagcgga aaactcggaa aatagataat
      361 ctgactgaaa aatcaggatt tctcgtcagg ttcctaattt tcagtcgttt tcttctcgct
      421 ctttgtatca taaattatgt ctatccatat tgctgctcag cagggtgaaa ttgctgataa
      481 aattcttctt cctggggatc ctcttcgtgc taagtttatt gcggagaatt tccttggtga
      541 tgctgtttgt tttaacgaag tgcgtaacat gtttggttac actggtactt acaagggtca
      601 ccgtgtatct gtcatgggaa ctgggatggg aatgccatct atttcgattt atgcgcgtga
      661 gttaatcgta gactacggtg tgaagaaatt gattcgtgtg ggaactgcag gttctttgaa
      721 tgaagaggtt catgttcgtg aattagtttt ggcgcaggcg gctgcaacca actcaaacat
      781 cgttcgtaat gactggccac agtacgattt tccacaaatt gctagctttg atttgcttga
      841 taaagcctac catatcgcca aaaaacttgg tatgactact cacgttggga acgttttgtc
      901 atctgatgtc ttttactcaa attactttga aaagaatatc gagcttggta aatggggagt
      961 caaggctgtg gaaatggaag cagcagctct ttactatctt gctgcccaat accatgttga
     1021 tgcgctagct atcatgacca tctctgatag cttggtcaat ccagacgaag acacaactgc
     1081 agaagaacgt caaaatacct tcactgatat gatgaaggtt ggtttggaaa ccttgattgc
     1141 agaataatta tagccaaaaa ggaagccctt tatgaattgt aggacttcct tttcttatcc
     1201 agaaattgat ctagctctct ctgatttcga agaatagtga ctttatgtga atattcttgg
     1261 caaagttttt ggtaattttc tttttgagtt ttgctacgcc catcccaaag aatccatctg
     1321 ataaactccc actcaaagcg ttcagggcaa tctgccgcca tactttctct gacttttcca
     1381 cggtatttaa gataacgctt aaaggctcta aagagacagg tcaatggcga aaaattgaga
     1441 aagatgattt ggtcagcttc ttgcattcgt tcttggtagt agcaccaaga ataattacca
     1501 tcgatgaccc aagctttatg cttggtgaga aagtttttta tctcggttaa catccattcg
     1561 cagtcactgt cttgccaacc aggttgaaat tggagtgtgt ccatgtgcag ttttggaatg
     1621 gagtagtagt tagataactt ttctgctaga gttgacttac cagaaccaga atatccgata
     1681 attgcgattt tcattttcta ccttttccta tttggagaca aaaaaacagc ctctatggac
     1741 tgtttcttat ttagcaagtt tagctgaaag acgagcttta tcgcggcttg ctttgttttt
     1801 gtgaatcaaa cctttagttt ctgctttatc tatagctgag ctagcagcac ggaaaagttc
     1861 ttcagatggg tttgcttcga aagcttttat agcagtacgc atagctgatt tttgagctga
     1921 gttcttttcg ttttgtttaa cgttcaattc agcgcgtttg atagctgatt taatgtttgc
     1981 caatgttctt acctccatat ttactaacta taccattata tatgaaaact tacgttttga
     2041 caagggaaaa ttactttttt aagtaaattt catcgatttc atggttcttg cttttatgaa
     2101 gaatcacttc tgcacgattt ctggttggtt caatataatt ttgcagattt gtgaggttga
     2161 tactgctcca gacctgatgg gcaaaggact ccacttcccc aatcggcatc tgagtaaaac
     2221 gataatagta gctatcaggg tcgttttggg ctaggctcag cattttcaag aaacggtcca
     2281 gataccaact ttcaatatca tccactccag catctacata gatggaaaag tcaaagaagt
     2341 cagtgatata gagacgatcg ttttgtggat tttgaaagac attaattccc tcaacaatta
     2401 caaaatcagc agctttgaca ctttgtttct tttcgggtac gatgtcgtaa acttcatgag
     2461 aatagacagg aatatctaca tcttgtccat ttttgatgcg gtccaagaag ttgagaagag
     2521 cttccatatc atagctttca ggaaatcctt tacgatttaa actcccctgc tcaatcaagg
     2581 tttgattggg atagagaaaa ccatcagttg taaccaactc aaccgtagca tctgtaaacg
     2641 tacgggacag taggatttga agtaggcgac tggttgtgga ttttccaacg gcaacactcc
     2701 cagaaacccc aataataaaa ggttgagatt tactttcacg ttggaggaaa attccttttg
     2761 aaaaggctaa atcttcctta gttcgcttgt aaatctgaat cagatgagcc aaggggagat
     2821 agatatctgt aacgtcttgg agactgattt ggtcattaaa actcttgata gattccaatt
     2881 cttcttctgt caaaggaggt gttgtcttgc gatgtaaaga ttgccaagtc tggcggctga
     2941 ttttttcaaa atgtaaaaat tcgttggtca tttgttttcc cctagatatt ttgtctatca
     3001 taccataaaa agctaaaaaa ataaagcggt ttcttgaatg tggtaagtca atgatatata
     3061 gtataagtga tcaatttatt gatgaaatag gagatagatt ctactgattt ataagcttat
     3121 aaaggaggct ttttagttca caacttatag gaggtgtact atgaaaatct taaaacgtta
     3181 catattggaa ctctgtttta ttttaagttt tgctttacct tttataaaag gaaccaatgc
     3241 agataatggt agatgctttg tggaaaccta ttacggtttt acttttttga tggaacatgc
     3301 tattgtaaca gctgtcttta tctgttcgtt cttaattgct ttcttactaa aaaacgatgg
     3361 gcgaaatgga ttgctgctgg tagttattgc tttttagtcc tatggattgc gacagagggt
     3421 tatttcttcc atatgtcacc agaagatttg atacgacttt ggacaagttt ggaaatcctg
     3481 acacaaacgt atcagttggg cttttatcta aacattttgt taggaatttt gttgatgatt
     3541 aagcatttga aggttaagca atagaaataa taatctgcct gattatagat gataaacttt
     3601 ttgtttttat ctggactgtg atatttatag ttctaaatgg tttattattt ataagtgttg
     3661 aaagagattg aagaaaatgg cttcgtgact cgctttcttc ggtctttttc gttttattat
     3721 caatttgtaa acgatttaca atttgagtca aattatggta aaatagtgtc atgagtaaaa
     3781 tgtattatgc agaaaatcct gacactgctc acgacattca tgagttgaga gtagagttgt
     3841 tgggacaaaa aatgaccttt ttaacggatg cgggtgtttt tagcaagaaa atagttgact
     3901 ttggaagtca actattgctc aagtgtcttg aggtcaacca aggagaaact gtccttgatg
     3961 taggctgtgg ttacggacct ttgggtttgt ccttagctaa ggcttatgga gttcaggcta
     4021 ctatggttga tatcaacact cgtgccttgg atttagctcg gagaaatgct gaaaaaaata
     4081 atgcaaaagc gacgattttc cagtccaaca tctatgaaca agttgaagga cattttgatc
     4141 atgtcatttc caatcctcct atccgtgcgg gcaagcaagt cgttcatgaa atcattgaga
     4201 aaagtaaaga tttcttggaa actggtgggg atttgacaat cgtcatccag aaaaaacaag
     4261 gggctccaag tgctaagtcc aagatggagg atgtgtttgg taattgtgaa atccttaaaa
     4321 aagacaaagg atactatatc cttagaagtg tgaaagaatg agagcagttg atttaatcca
     4381 aaaaaaacga gacggtcaag aactgacttc aagtgaaatt gaatggctgg tagaaggcta
     4441 tgtgtcagga actgttcctg attatcagat gtctgctttt gctatggctg tttattttaa
     4501 aggaatgacg actcgtgaaa tttctgattt aacgatgaat atggttaaga ctgggcaaga
     4561 gtttgacctg tcagccattg atggtgtgaa agttgacaag cattctactg gtggtgtcgg
     4621 cgataaggta accttgattt tagctcctct tgttgctagc ttcggtgtgc ctgtagcaaa
     4681 aatgagtggt cgtggtcttg gccatacagg cggaacaatt gataaattgg agtccattag
     4741 gggctatcaa gtagaacgta gtcaagagga ttttattcgt caggttcagg atattggagt
     4801 atctgtcatt gggcaatcag accagctggt caaagcagat aagcttctct atgcccttcg
     4861 tgatgtgacc gcaactgtcg acacgattcc tttgattgcg agttcggtga tgagcaagaa
     4921 aattgcggca ggggcggatg ctattttgct agacgtgact gtcggtgagg gtgccttcat
     4981 gaagacggtt gatgaggcgc gtgagttggc tcaaactatg gtagatcttg gtaaggttgt
     5041 tggacggaag acaatagcag tgattacgga tatgagccag cctttgggac gagcgattgg
     5101 aaatcgtctg gaaatccttg aagcattgga gattttgcaa ggtcaaggac gtcaggatat
     5161 tacccacttt atttgtgaat tggctcaaat tatgcttggt ttggcaaatg ttaacaagac
     5221 tgttgaagaa gttcaccaac atcttgagaa tggtcaagca ctggctaagt ttgaggaaat
     5281 ggtgcaagcc caaggtggag acttggaaga cctctatcgt cctgtaaatg tagctcatgt
     5341 ggtggaaatc cctgctcagg aaacgggtgt catttcagct cttccagcta tggactttgg
     5401 tctttatgcc atgaggctgg gtgctggtcg tgcagttaag tctgatgcct tggactatga
     5461 aacaggaatt gtttttgaaa agaagattgg agactccgtt caaaagggtg aaattgttgc
     5521 aaaagtatac acaaatgaaa aaattccacc tcaactagtt acagatttcc aaaaatgtgt
     5581 taaaataagt gatgaagtga aaaaaatccg tgaaattgta gaaatcatct cctaaatgga
     5641 gggaaaaccg aaatgaaatt aaataaatat atagatcata cgcttttaaa acaagatgca
     5701 aagaaaaaac aaattgatag tttgttgtct gaggctagag agtatgactt tgccagtgtt
     5761 tgcgttaatc cgacctgggt tgaacatgct aaaaaaggac ttgaaggcac agatgttaag
     5821 gtttgcacag tagtaggttt ccctttggga gcaacaactt cagccgtgaa agcatttgag
     5881 acaaaagaag ctatccaaaa tggtgcagat gagattgata tggtgatcaa tgttggagct
     5941 ctcaaatcag gtaatttagc cttggttgag tcagatattc gcgcagtagt ggaagcaagt
     6001 ggtgataagt tagtgaaagt cattattgaa gcttgccttc tgacagacca agaaaaagtt
     6061 gttgtttgcc aattggccca aaaagctggg gctgactttg tcaaaacatc tactggcttt
     6121 tcaactggtg gtgctacgat agcagatgtt acattaatgc gtgaaacagt tggatctgat
     6181 atgggtgtca aggccgccgg tggagctcgt tcttatgcag atgctcttgc ctttgtcgaa
     6241 gcaggtgcga cccgtatcgg aacgtcagct ggggtagcta ttttaaaagg agaattggca
     6301 gatggcgact actgagttga ttgaactggc aattgaaacc agcaaacatg cctatgtccc
     6361 ctattctcac tttcctatcg gagcagtttt agtagctaaa gacggaagtg tttacacggg
     6421 agtgaatatc gaaaatgcta gctatccttt gaccaattgc ggtgagagaa cagcagtttt
     6481 taaggctata tctgaaggcc aaagagaatt ttcagaattg attgtctatg gtcaaactga
     6541 aaaaccgatc tcgccatgtg gtgcttgtcg ccaagtaatg gtcgaatttt ttgaacaaga
     6601 tttaaaagtg accttagtcg caaaagataa atcgacggtc gagatgacgg tcggggagtt
     6661 acttccatac tcttttacag acttaaacta gtctgagtcg ctctttgagt ggcacggtcc
     6721 ttgtggccaa ccaatccata cttgcaacat cgttgcacat cttatttagg aggttcagta
     6781 atgaacaaga aacaatggct aggccttggt ctagttgcag tggcagcagt tggacttgct
     6841 gcatgtggta accgctcttc tcgtaacgca gcttcatctt ctgatgtgaa gacaaaagca
     6901 gcaatcgtca ctgatactgg tggtgttgat gacaaatcat tcaaccaatc agcttgggaa
     6961 ggtttgcaag actggggtaa agaacacaat ctttcaaaag ataatggttt cacttacttc
     7021 caatcaacaa gtgaagctga ctacgctaac aacttgcaac aagcggctgg aagttacaac
     7081 ctaatctttg gtgttggttt tgcccttcac aatgcagttg aagaagcagc taaagaacac
     7141 actgacttga actatgtctt gattgatgat gtgattaaag accaaaagaa tgttgcgagc
     7201 gtaactttcg ctgataatga gtcaggttac cttgcaggtg tggctgcagc aaaaacaact
     7261 aagacaaaac aagttggttt tgtaggtggt atcgaatctg aagttatctc tcgttttgaa
     7321 gcaggattca aggctggtgt tgcgtcagta gacccatcta tcaaagtcca agttgactac
     7381 gctggttcat ttggtgatgc ggctaaaggt aaaacaattg cagccgcaca atacgcagcc
     7441 ggtgcagata ttgtttacca agtagctggt ggtacaggtg caggtgtctt tgcagaggca
     7501 aaatctctca acgagagccg tcctgaaaat gaaaaagttt gggttatcgg tgttgaccgt
     7561 gaccaagaag cagaaggtaa atacacttct aaagatggca aagaatcaaa ctttgttctt
     7621 gtatctactt tgaaacaagt tggtacaact gtaaaagata ttgctaacaa ggcagaaaaa
     7681 ggagaattcc ctggcggtca agtgatcgtt tactcattga aggataaagg ggttgacttg
     7741 gcagtaacaa acctttcaga agaaggtaaa aaagctgtcg aagatgcaaa agctaaaatc
     7801 cttgatggaa gcgtaaaagt tcctgaaaaa taatggatgg aagtcatttc ttaggaagcg
     7861 gccaagggcc gcttttttaa gagttgagac aaagtcattt tgtctcagta aagcttgcta
     7921 ctagacaaac tagcaagttt tattgaaata aaataagact ctgaaaggaa gagcacatgg
     7981 cacacgaaaa tgtcattgag atgcgtgata ttaccaaggt gtttggtgga tttgttgcca
     8041 acgacaaaat caacttgcac ctacgaaaag gtgaaattca tgcactttta ggagaaaatg
     8101 gggctggtaa gtccacgcta atgaacatgt tagcaggcct tcttgaacca actagtggtg
     8161 aaatcgcggt caacggtcaa gttgtcaatc tcgactcccc atctaaagca gctagcttgg
     8221 gaatcgggat ggttcaccag cactttatgt tggttgaagc cttcacagtg gttgaaaaca
     8281 tcattttagg tagtgaattg actaaaaatg gtgtgctaga tatcgctgga gctagcaaag
     8341 aaatcaaggc tctttctgaa cgttatggct tagctgttga cccttctgcc aaggtagcag
     8401 atatctcagt tggagcccaa caacgtgtag aaattttaaa aacactttat cggggggctg
     8461 atatccttat ctttgacgaa ccaacggctg ttttgactcc atcagaaatt gatgagttga
     8521 tggctattat gaaaaatctt gtcaaagaag gaaaatcaat tatcttgatt acccacaaat
     8581 tggatgaaat tcgcgcagtt tctgaccgtg ttacagttat ccgtcgtggg aaatcaattg
     8641 aaaccgttga aattacaggg gctaccaatg ctgatttggc ggaaatgatg gtaggacgtt
     8701 ctgtttcctt taaaacagag aagcaagcct ctaaaccaaa agaagtggtt ttgtctatca
     8761 aagatttggt ggtcaatgaa aaccgtggtg ttccagctgt taaaaatctg tccttggatg
     8821 ttcgtgctgg agagattgtt ggtattgcgg ggattgatgg aaatggtcag tctgaactga
     8881 ttcaagccat tacaggtctt cgtaaggttg aatctggtag cattgagcta aaaggagatt
     8941 caattgtagg cttgcaccca cgtcagatta cagaactaag tgttgggcac gttccagaag
     9001 accgtcaccg tgatggcttg attttggaaa tgatgatatc tgaaaatatt gcccttcaaa
     9061 cctactataa agaaccacat agtaaaaatg gaattttaaa ctattcaaat attacttctt
     9121 atgctaaaaa gctgatggaa gagtttgatg ttcgcgttgc cagtgaatta gttcctgcag
     9181 ctgcactctc aggaggaaat caacaaaaag caattattgc tcgtgaaatt gatcgagttc
     9241 ctgatctcct tatcgttagc cagccaactc gtgggttgga tgtcggtgcc attgagtata
     9301 tccacaaacg cttgattgaa gagcgtgata atggcaaggc tgtccttgtt gtcagctttg
     9361 aattggatga gattttaaac gtctcagacc gtattgccgt tatccacgat ggtaagattc
     9421 aaggtattgt atcaccagaa acaaccaata aacaagaact tggtgtcttg atggctggtg
     9481 gaaacttggg aaaggagaag agtgatgtct aaaaaattac aacaaatttc ggttcccttg
     9541 atttctgtat tcctaggaat tttactcgga gccattgtca tgtggatctt cggttatgat
     9601 gctatttggg gctacgaaga attgttctat acagcctttg gcagtctgcg tgggattgga
     9661 gaaatcttcc gtgctatggg tcctctggtc ttgattggtc ttggttttgc cgttgccagt
     9721 cgagctggtt tctttaacgt cggacttcct ggtcaggctt tggcaggttg gattctcagt
     9781 ggttggtttg ccttatcgca tccagatatg ccccgtccct tgatgattct ggcaactatt
     9841 gtgattgcct tgattgctgg tgggcttgtc ggagcgattc caggtattct tagggcctat
     9901 ctagggacgt cagaggttat tgtaactatc atgatgaact acattgtctt gtatgtagga
     9961 aatgccttta tccatgcctt ccctaaagac tttatgcaaa gtacagattc gaccattcgt
    10021 gttggggcta atgcaaccta ccagacacct tggttgtctg agttgactgg taactcacgg
    10081 atgaatattg gtattttctt tgccatcatt gccgttgcag tcatttggtt catgctcaag
    10141 aaaacgactc ttggttttga aattcgcgca gttggtctca atccacatgc ttcagaatat
    10201 gcgggtattt ctgcaaaacg gacgattatc ctctcaatga ttatttcagg tgccttggca
    10261 ggtcttggtg gtgctgttga aggacttgga accttccaga acgtctatgt tcaaggggca
    10321 tcattagctg ttggatttaa cggaatggcg gttagtctgc ttgcagccaa ctcaccaatc
    10381 ggtattctct ttgcagcctt cctatttggt gttctccaag ttggagcccc tggtatgaat
    10441 gcggcgcagg taccgtctga gcttgttagc attgtaacag cgtctattat cttctttgtc
    10501 agtgttcatt accttatcga acgctttgtc aaaccgaaaa aacaagttaa aggaggtaag
    10561 taaagatgtc tattacaacc ttgctcaccc ttttggtatc ttctatgctg atttactcag
    10621 cacccctcat ctttacaagt attggtggtg ttttctctga acgtggtggt gtggtaaacg
    10681 tcggccttga aggaattatg gttatgggtg ccttttctgg agttgtcttt aaccttgaat
    10741 ttgcagaaca atttggagca gcaactccat ggctatcttt gctagttgca ggattggttg
    10801 gatgcctctt ctctatcatc cacgcagcag cgacggttca tttccgtgca gaccatgttg
    10861 tcagcggtac ggtattgaac ttgatggcgc cagccttggc tgttttctta gtcaaagttc
    10921 tttataacaa aggacaaacc gacaacctaa gtcagacttt tggacgcttt gacttcccag
    10981 tcttggcaaa tatcccagtg attggtgata tcttcttcaa gtcaactagt ctgcttggtt
    11041 atatcgcgat tgccttctca ttccttgctt ggtttatcct cttcaagacc cgatttggtc
    11101 ttcgtctccg ctctgtaggt gaacatcctc aagcagcgga taccttggga atcaatgtct
    11161 acaagatgag atatctagga gttatcattt caggtttcct aggtggaatt ggcggagcga
    11221 tttatgctca atccatctca gttaacttct cggtaacaac tattgttgga cctggattta
    11281 ttgctcttgc tgcgatgatt ttcggaaaat ggaatccaat cggagccatg ctatctagtc
    11341 tcttctttgg gctttcacaa agtttggctg ttatcggttc tcaattgccg ttcctacaag
    11401 gagtgcctac agtttatctt caaatcgcac cttatgtttt gacaattctt gtctcagcag
    11461 ccttctttgg aaaagcagtc gcaccaaaag cagatggtat caactacatc aaatcaaaat
    11521 aagcatacaa aaaaacgtca gtccaatgac tgaactgc
//
LOCUS       DAVKDZ010000065        11352 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000065 DAVKDZ010000000
VERSION     DAVKDZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11352
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.066"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(93..1723)
                     /locus_tag="VWZ30_001655"
                     /pseudo
     CDS             complement(93..1723)
                     /locus_tag="VWZ30_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
     gene            2012..6887
                     /locus_tag="VWZ30_001656"
                     /pseudo
     CDS             2012..6887
                     /locus_tag="VWZ30_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
     gene            6977..8173
                     /locus_tag="VWZ30_001657"
     CDS             6977..8173
                     /locus_tag="VWZ30_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU9335352.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELET
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TVGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8309..8836
                     /locus_tag="VWZ30_001658"
     CDS             8309..8836
                     /locus_tag="VWZ30_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9335353.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDKSVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            8913..9269
                     /gene="glnR"
                     /locus_tag="VWZ30_001659"
     CDS             8913..9269
                     /gene="glnR"
                     /locus_tag="VWZ30_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEU9335354.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9306..10652
                     /gene="glnA"
                     /locus_tag="VWZ30_001660"
     CDS             9306..10652
                     /gene="glnA"
                     /locus_tag="VWZ30_001660"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU9335355.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKAMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLHGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVRAALGEHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10748..10867)
                     /locus_tag="VWZ30_001661"
     CDS             complement(10748..10867)
                     /locus_tag="VWZ30_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU9335356.1"
                     /translation="MPDIVFKIEFFHSDSLIFFYTSDDKSNLQKSQEDFSKNK"
     gene            11125..11262
                     /locus_tag="VWZ30_001662"
     CDS             11125..11262
                     /locus_tag="VWZ30_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335357.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEVLNIRLEVK
                     N"
BASE COUNT         3624 a         2078 c         2459 g         3191 t
ORIGIN      
        1 atctgtagaa tactctttca gtttcattca gcctagtctt gcttagaaaa aagtaaaaag
       61 ccatccaatt atgatggata gcttcttggt tcttagattt gttctgcaaa aaccttttca
      121 gcaaggttca tagcgtggtc tgatacacga gtgtagtgtg agatgatgtc gataaagttg
      181 accccagctt gtgttgaaca ttcgcctttg ttgaggcgtt tgatgtgggt ttttctgaga
      241 acacgttcta tcttattgat tgtttcatga cgttctacaa gactgcgtgc tttttcaata
      301 tcattgtttt ccacactatc cagagcatct ttgataaagt cactagtttg gcggtaaact
      361 tcctctaatt ctttcaaggc ggcatcagaa aattcaacat ttttccgttg aagataggca
      421 gtcagattga gtagagcctc cgtgtggtct ccaatccgtt ccaaatcacg ggaggaatca
      481 aggatattgg taagcacttc actttctttt tggctgagag cttcgcttga aagggcaatg
      541 agataacgtg ttaattgctc atcgatggtg ttaattgctt cttcggtttt atgccctttt
      601 tcagcaactt tttcatccaa gtcaatgatg tacttatagg aaaggtcaaa ggctttagca
      661 gcgtagtttc ctaagtgcaa gagctctttc ttagcatttc ctagagcgat agatggggcc
      721 tgtttgatga aatgttcatc aagatataag ggttcgtatt tgacaacctc gtcctctcca
      781 ggaataatct tggttacaaa gtaagccaga gctccgataa atggaaattg gacaatggtg
      841 ttggtaatat taaaggttcc gtgagcaaag gcgatggtca tttccggtgc tagatttagc
      901 gtagcttcaa accaatgaat caggacagta aaaggaacta gaaaaataac gcagacgact
      961 gttccgataa cgttgaaggc aacatgagct cctgctaccc gtttagctgc aatattagcc
     1021 cctaaagagg caatgatggc tgtaatggtt gtcccgatat tgtcaccaaa tagaactggc
     1081 aaagctccct gtaggtcaat tagattgccg gcgtagaggt tttgtaaaat cccaatggta
     1141 gccgaagaag cttgaattag caaggtcaag ccggtaccga caaagacacc caaaacagga
     1201 ttcttactta gctcaatcat atagtcctta aagacctgta aatccttgag tggagtcatt
     1261 gcgccgctca tgagattgag ggcaaaaaag ataccaccga caccaaagag gatacgtccg
     1321 atattattga ctgtccattt tttgtaaaaa agagacagac ggcaccgata aagagcatag
     1381 gtagggcata gttacctaat ttaaaaccga tgagaaagga tgtgacagtt gtcccaatat
     1441 tagcacccat gacaatcccg atagcctgac gtaaggttaa gagaccggca ctgaccaggc
     1501 cgactgtgat aactgttaca ccagaactag actgaattag agcagtcatc ccaataccaa
     1561 ccagaactcc aaagaaagga ttactagtat atttgtcaat gtaaaaacga aggcgatctc
     1621 cagcagcttg ttgcaaaccg tctcccatgg tcttgatact atataagaat agccccagac
     1681 cacctaaaaa gtgaaataaa atttcctgcc aattaatgga catttctttt tcctccgaaa
     1741 aataatcgcg gaatatctcc tattctattt taaaggataa aagtaaatct aacaagtgtt
     1801 aatgtcaagt ttgtaaaaga aaattataat tgataagtgt attattttta agagttttta
     1861 accttatttt agatttgtga aaacagctat tgttttttgt ctaaaaatgt tttaggataa
     1921 aaatgtgatt atcatggttc tagagttaaa aaactacaga atataaagcg ctttcttgaa
     1981 aacaacaaaa tcaatctttt aggaggagaa aatgaagaat ccattttttg aaagacgttg
     2041 tcgttacagt attcgtaagt tatcagtagg agcctgctcg ctgatgattg gtgctgtttt
     2101 atttgctggt ccagccttgg ctgaagaaac tgcagttcct gaaaatagcg gagctaatac
     2161 agagcttgtt tcaggagaga gtgagcattc gaccaatgaa gttgataagc agaatgaagg
     2221 ggaacatgct agagaaaaca agctagaaaa ggcagaagca gctagcgtag ctaaaccaga
     2281 ggaaaaagca ggtgaggtgg ttgcagaaac accatctgca gaagcaaagc ccgaagcaac
     2341 taaccaaggg gatgagtcta agccagcagc agaagctaat aagactgaaa aagaagtcca
     2401 gccagatgtc cctaaaaata cagaaaaaac attaaaacca aaggaaatca aatttaattc
     2461 ttgggaagaa ttgttaaaat gggaaccagg tgctcgtgaa gatgatgcta ttaaccgcgg
     2521 atctgttatc ctcgcttcac gtcggacagg tcatttagtc aatgaaaaag ctagcaagga
     2581 agcaaaagtt caagccttat caaacaccaa ttctaaagca aaagaccatg cttctgttgg
     2641 tggagaagag ttcaaggcct atgcttttga ctattggcaa tatctagatt caatggtttt
     2701 ctgggaaggt ctcgtaccaa ctcctgacgt tattgatgca ggtcaccgta atggggttcc
     2761 tgtatacggt acactcttct tcaactggtc taatagtatt gcagatcaag aaagatttgc
     2821 tgaagctttg aagcaagacg cagatggtag cttcccaatt gcccgtaaat tggtagacat
     2881 ggccaagtat tatggctatg atggctattt catcaaccaa gaaacaactg gagatttggt
     2941 taaacctctt ggagaaaaga tgcgccagtt tatgctctat agcaaggaat atgctgctaa
     3001 ggtaaaccat ccaatcaagt attcttggta tgatgccatg acctataatt atggacgtta
     3061 ccatcaagat ggtttgggag aatacaacta ccaattcatg caaccagaag gagataaggt
     3121 tccggcagat aacttctttg ctaactttaa ctgggataag gctaaaaatg attacactat
     3181 tgcaactgcc aactggattg gtcgtaatcc ttatgatgta tttgcaggtt tggaattgca
     3241 acagggtggt tcctacaaga caaaggttaa gtggaatgac attttagacg aaaatgggaa
     3301 attgcgcctt tctcttggtt tatttgcccc agataccatt acaagtttag gaaaaactgg
     3361 tgaagattat cataaaaatg aagatatctt ctttacaggt tatcaaggag accctactgg
     3421 ccaaaaacca ggtgacaaag attggtatgg tattgctaac ctagttgcgg accgtacgcc
     3481 agcggtaggt aatactttta ctacttcttt taatacaggt catggtaaaa aatggttcgt
     3541 agatggtaag gtttctaagg attctgagtg gaattatcgt tcagtatcag gtgttcttcc
     3601 aacatggcgc tggtggcaga cttcaacagg ggaaaaactt cgtgcagaat atgattttac
     3661 agatgcctat aatggcggaa attcccttaa attctctggt gatgtagccg gtaagacaga
     3721 tcaggatgtg agactttatt ctactaagtt agaagtaact gagaagacca aacttcgtgt
     3781 tgcccacaag agaggaaaag gttctaaagt ttatatggca ttctctacaa ctccagacta
     3841 caaattcgat gatgcagatg catggaaaga gctaaccctt tctgacaact ggacaaatga
     3901 agaatttgat ctcagctcac tagcaggtaa aaccatctat gcagtcaaac tatttttcga
     3961 gcatgaaggt gctgtaaaag attatcagtt caacctcgga caattaacta tctcggacaa
     4021 tcaccaagag ccacaatcgc caacaagctt ttctgtagtg aaacaatcgc ttaaaaatgc
     4081 ccaagaagcg gaagcagttg tgcaatttaa aggcaacaag gatgcagatt tctatgaagt
     4141 ttatgaaaaa gatggagaca gctggaaatt actaactggc tcatcttcta caactattta
     4201 tctaccaaaa gttagccgct cagcaagtgc tcagggtaca actcaagaac tgaaggttgt
     4261 agcagtcggt aaaaatggag ttcgttcaga agctgcaacc acaacctttg attggggtat
     4321 gactgtaaaa gataccagcc taccaaaacc actagctgaa aatatcgttc caggtgcaac
     4381 agttattgat agtactttcc ctaagactga aggtggagaa ggtattgaag gtatgttgaa
     4441 cggtaccatt actagcttgt cagataaatg gtcttcagct cagttgagtg gtagtgtgga
     4501 tattcgtttg accaagccac gtaccgttgt tagatgggtc atggatcatg caggggctgg
     4561 tggtgagtct gttaacgatg gcttgatgaa caccaaagac tttgaccttt attataaaga
     4621 tgcagattgt gagtggaagc tagctaagga agtccgtggt aacaaagcac atgtgacaga
     4681 tatcactctt gataaaccaa tcactgctca agactggtac ttgaatgttg tcacttctga
     4741 caatggaact ccatggaagg ctattcgtat ctataactgg aaaatgtatg aaaagcttga
     4801 tactgagagt gtaaatattc cgatggccaa ggctgcagcc cgttctctag gcaataacaa
     4861 ggtacaagtt ggctttgcag atgtaccggc tggagcaact attaccgttt atgataatcc
     4921 aaattctcaa actccgctcg caaccttgaa gagcgaagtt ggaggagacc tagcaagtgc
     4981 accattggat ttgacaaatc aatctggtct tctttattat cgtacccagt tgccaggcaa
     5041 ggaaattagt aatgtcctag cagtttccgt tccaaaagat ggcagaagaa tcaagttagt
     5101 cagcctagaa acaggaccta agaaaataag ctacgccgaa ggggaggatt tggaccttag
     5161 aggtggtgtt cttcgagttc agtatgaagg aggaactgag gacgaactca ttcgtctaaa
     5221 tcacgcaggt gtatcagtat caggttttga tacgcatcat aagggagaac agaatcttac
     5281 tctccaatat ttgggacaac cagtaaatgc taatttgtca gtgactgtca ctggccaaga
     5341 tgaagcaagt ccgaaaacta ttttgggaat tgaagtaagt caggaaccga aaaaagatta
     5401 cctagttggt gatagcttag acttgtctga aggacgcttt gcagtggctt atagcaatga
     5461 caccatggaa gaacattcct ttactgatga gggagttgaa atttctggtt acgatgctca
     5521 aaagactggt cgtcaaacct tgacgcttcg ttaccaaggt catgaagtca actttgatgt
     5581 tttggtatct ccaaaagcag cattgaacga tgagtacctc aaacaaaaat tagcagaagt
     5641 tgaagctgct aagaacaagg tggtctataa ctttgcttca ccagaagtaa aagaagcttt
     5701 cttgaaagca attgaagcgg ccgaacaagt gttgaaagac catgaaacta gcacccaaga
     5761 tcaagtcaat gaccgactta ataaattgac agaagctcat aaagctctga atggtcaaga
     5821 gaaatttatg gaagaaaaga cagagcttga tcgcttaaca ggtgaggctc aagaactctt
     5881 ggctgccaaa ccatccttca ggttctgccc tagctccgct tcttgaaaag aacaaggcct
     5941 tggttgaaaa agtagatttg agtccagaag agcttgcaac agcgaaacag agtctaaaag
     6001 atctggttgc tttattgaaa gaagacaagc cagcagtctt ttctgatagt aaaacaggtg
     6061 ttgaagtaca cttctcaaat aaagagaaga ctgtcatcaa ggatttgaaa gtagagcgtg
     6121 ttcaagcaag tgctgaagag aagaaatact ttgctggaga agatgctcat gtctttgaaa
     6181 tagaaggttt ggatgaaaaa ggtcaagatg ttgatctctc ttacgcttct attgtgaaaa
     6241 tcccaattga aaaagataag aaagttaaga aagtattttt cttacctgaa ggcaaagagg
     6301 cagtagaatt ggcttttgaa caaacggata gtcatgttat ctttacagca ccacacttta
     6361 ctcattatgc ctttgtttat gaatctgctg aaaaaccaca acctgctaaa ccagcaccac
     6421 aaaacacagt ccttccaaaa cctacttatc aaccggcttc tgatcaacaa aaggctccta
     6481 aattggaagt tcaagaggaa aaggttgcct ttcatcgtca agagcatgaa aatgctgaga
     6541 tgctagttgg ggaacaacga gtcatcatac agggacgaga tggactgtta agacatgtct
     6601 ttgaagttga tgaaaacggt cagcgtcgtc ttcgttcaac agaagtcatc caagaagcga
     6661 ttccagaaat tgttgaaatt ggaacaaaag taaaaacagt accagcagta gtagctacac
     6721 aggaaaaacc agctcaaaat acagcagtta aatcagaaga agcaagcaaa caattgccaa
     6781 atacaggaac agctgatgct aatgaatccc taatagcagg cttggtcagc cttggtcttg
     6841 ctagtttagc cttgaccttg agacggaaaa gagaagataa agattaaata tcgaaaaatc
     6901 ttgtgaaatc tttccttata tttccaaagt atgatataat agtttcgaat aaaataaata
     6961 aaggagtttt tgtaacatgg caaaacttac tgttaaagac gttgacttga aaggtaaaaa
     7021 agtcctcgtt cgtgttgact tcaacgtacc attgaaagat ggcgtaatca ctaacgataa
     7081 ccgtatcaca gcagctcttc caactattaa gtacatcatc gaacaaggtg gacgtgcaat
     7141 tcttttctct caccttggac gtgtgaaaga agaagctgat aaagctggta aatcacttgc
     7201 tcctgtagca gctgacttgg cagcaaaact tggtcaagat gttgttttcc caggtgtcac
     7261 tcgtggtgcg gaattggaaa cggcaatcaa cgctcttgaa gatggacaag ttctcttggt
     7321 tgaaaacact cgttacgaag atgttgacgg caagaaagaa tctaaaaacg atcctgaact
     7381 tggtaaatac tgggcatcac ttggagatgg tatcttcgta aacgatgcat tcggtacagc
     7441 tcaccgtgca cacgcatcta acgttggtat ctcagcaaac gttgaaaaag cagttgctgg
     7501 tttccttctt gaaaacgaaa ttgcctacat ccaagaagca gttgaaactc cagaacgtcc
     7561 attcgtggct atccttggtg gttcaaaagt ttcagacaag atcggtgtta tcgaaaactt
     7621 gcttgaaaaa gctgataaag tccttatcgg tggtgggatg acttatacat tctacaaagc
     7681 acaaggtatc gaaattggta actcacttgt agaagaagac aaattggatg ttgcgaaagc
     7741 tcttcttgaa aaagcaaatg gtaaattgat cttgccagtt gactcaaaag aagctaacgc
     7801 atttgctggt tacactgaag tgcgtgacac tgaaggtgaa gcagtttctg aaggcttcct
     7861 tggtcttgat atcggtccaa aatctatcgc caaatttgac gaagctttga ctggtgccaa
     7921 aacagttgta tggaacggac ctatgggtgt atttgaaaac ccagatttcc aagctggtac
     7981 agtcggtgtg atggacgcta tcgtgaaaca accaggagtt aaatcaatca tcggtggtgg
     8041 tgactcagct gccgcagcga ttaaccttgg ccgtgcagac aagttctcat ggattagtac
     8101 gggtggtgga gcatcaatgg aacttcttga aggtaaggtt cttccaggac ttgcagcctt
     8161 gacagaaaaa taagatttta taaacaaatc aaagaagaga gggatgaaag ttcctctttt
     8221 cttttgctta aaataaaaac gcttcctctc aactattact cataaaaatc accgatttat
     8281 gataaaatgg aaatagaaag ttgagattat gagttatttt aaaaaatata aattcgataa
     8341 atcccagttt aaacttggta tgcgaacctt taaaacaggt attgctgttt ttctagttct
     8401 cttgattttt ggattttttg gctggaaagg tcttcaaatt ggtgctttga cagccgtttt
     8461 tagcctgagg gagagttttg ataagagtgt tcattttggg acttcgcgta ttctaggaaa
     8521 tagtatcggt ggactctatg ccttggtctt cttcttatta aatacctttt tccacgaagc
     8581 cttttgggtg accttggtag ttgttccaat ctgcaccatg ttaaccatta tgacaaatgt
     8641 agccatgaac aacaaagcag gagttattgg tggtgtagca gctatgttaa tcattaccct
     8701 atcaattcca agtggtgaga caatttcgta cgtgtttgtg cgtgtatcag aaacgtttat
     8761 gggagttttt gtcgcaatta tcgtaaatta cgatattgat cgtattcgtc tcttcttaga
     8821 gaaaaaagaa aaataatgtt acattttata acattattaa ttgacgtttg tcttttttta
     8881 gactataata gacagaaaga aggaaattgt aaatgaagga aaaagaattt cgccgaaata
     8941 tggctgtttt tcctatcggc agtgttatga agttgaccga tctatcggcg cgtcagattc
     9001 gttattatga agatcaagag ttgatcaagc ccgatcgaaa cgaaggaaat cgtcgcatgt
     9061 attccttgaa tgacatggat cgtctgcttg aaatcaaaga ttatatctct gaaggttata
     9121 atatcgctgc cattaagaaa aaatatgctg aacgtgaagc gaaatccaag aaagcggtga
     9181 gtcagactga ggtgcgccgt gcacttcaca atgaactcct ccaacagggt cgctttgctt
     9241 cagtacagtc accttttggt cgcggttagg caaccgcaag tagtcataca ttaaggagaa
     9301 aactcatgcc aatcacagct gcagatattc gtcgtgaagt caaggaaaaa aatgttacct
     9361 ttattcgtct catgttttca gatattttgg gaaccatgaa aaacgtcgaa attcctgcta
     9421 cagatgaaca attagataag gtcttgtcga acaaggctat gtttgatgga tcttctattg
     9481 aaggttttgt acgtatcaat gagtcggata tgtacttgta ccctgacttg gatacatgga
     9541 cagtcttccc ttggggagat gaaaatggaa gtgttgcagg tttgatctgt gatgtctata
     9601 caacagaagg tgaaccattt gcaggggacc ctcgtggtaa tttgaaacga gctcttcgtc
     9661 atatggaaga agtaggattc aaatctttca accttggtcc agagccagaa ttcttcctat
     9721 ttaaactgga tgaaaatgga gatccaacgc tcgaagtgaa tgacaagggt ggctactttg
     9781 atttggcgcc tactgacctt gcggacaata cacgtcgtga gattgtcaat gtcttgacca
     9841 aaatgggatt tgaagtagaa gccagtcacc acgaggttgc ggttggtcaa cacgagattg
     9901 actttaagta tgatgaagtt ctccgtgctt gtgataagat tcaaatcttt aaactcgttg
     9961 ttaaaaccat tgctcgcaaa catgggcttt acgcaacctt tatggcgaaa ccaaaatttg
    10021 gtattgctgg atcaggtatg cactgtaata tgtccttgtt tgatgcagaa ggaaataacg
    10081 ccttctttga tccaaatgat ccaaaaggaa tgcagttgtc agaaacagcc taccattttc
    10141 ttggcggttt gattaagcat gcctacaact atactgctat catgaaccca acagttaact
    10201 catacaaacg tttggttcca ggttatgaag cgcctgttta cattgcttgg gctggtcgta
    10261 accgttcgcc acttgtgcgc gtacctgctt cacgtggtat gggaactcgt cttgagttgc
    10321 gttcagtgga tccaatggcg aacccttaca tcgctatggc ggttctgttg gaggttggtt
    10381 tgcatggtat tgaaaataaa atcgaagcac cagctcctat cgaagaaaat atctacatca
    10441 tgacagcaga agagcgtaag gaagctggta ttacagacct tccatcaact cttcataacg
    10501 ctttgaaagc tttgacagaa gatgaagttg tcagagcggc tcttggagaa catatctata
    10561 ctagtttcct tgaagccaaa cgaatcgaat gggcaagtta tgcaaccttt gtttcacaat
    10621 gggaaattga taattattta gacctttact aatactaata tagaagaaag attgcctgtg
    10681 ggtaaacagt cacacagcag tctaagccat caaataccta taacccttaa aatagcgatt
    10741 tcggtcgcta tttatttttc gaaaaatcct cttgactctt ttgcaaatta gatttatcat
    10801 cagacgtgta gaagaaaatc aaggagtcac tatggaaaaa ttcaatttta aaaacaatat
    10861 cgggcaagaa aacaagttac ttcaaatcga aatctacaag tttactaact tttgcaagct
    10921 tcaaaattac acatctgtaa atattttttc gaaagatata tttgaagcca tagtaaattg
    10981 agactagaat agtacactac atctgctaaa aatttctaga aactaattta actttcctaa
    11041 tcgatttgtt tacatcttat ttcaatctac tatattatct caataaatga tgatggcgac
    11101 tgttttgttt gtgcttgtcg atttttgaat cctgatcgag cggaagaata tttctgccga
    11161 ggttgccaag gggaaaatcc tgaagatatt gaattttatg atgaacaatt acaggcagaa
    11221 aaggtggagg ttttgaatat tcgattggaa gtaaaaaatt gaattatcta caatctcatc
    11281 aacaataaac ctatttcaac cccattatct ataggcgtat ttttacgcct ttttatatac
    11341 ttgaagcaca ta
//
LOCUS       DAVKDZ010000066        11258 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000066 DAVKDZ010000000
VERSION     DAVKDZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11258
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.067"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            48..881
                     /locus_tag="VWZ30_001663"
     CDS             48..881
                     /locus_tag="VWZ30_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9335358.1"
                     /translation="MIEVSHLSKSFGDKIALNDISFTVKEGQIFGFLGPSGSGKTTTI
                     NILTGQLLADKGQSIILGQKSQNLTSGELKRIGLVSDTSGFYEKMSLYNNLLFYSKFY
                     NISKSRVDNLLKRVGLYDSRKMVAGKLSTGMRQRMLLARALINKPAVLFLDEPTSGLD
                     PTTSRTIHELILELKTAGTTIFLTTHDMNEASLLCDYVALLNKGKLVEQGAPSELIQR
                     YNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLEDIFITLTGGKLN
                     A"
     gene            874..1587
                     /locus_tag="VWZ30_001664"
     CDS             874..1587
                     /locus_tag="VWZ30_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9335359.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEEFSWNKTLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(1910..2002)
                     /locus_tag="VWZ30_001665"
                     /pseudo
     CDS             complement(1910..2002)
                     /locus_tag="VWZ30_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            2321..3022
                     /gene="pyrF"
                     /locus_tag="VWZ30_001666"
     CDS             2321..3022
                     /gene="pyrF"
                     /locus_tag="VWZ30_001666"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU9335360.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            3056..3688
                     /locus_tag="VWZ30_001667"
     CDS             3056..3688
                     /locus_tag="VWZ30_001667"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU9335361.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            3868..4271
                     /locus_tag="VWZ30_001668"
                     /pseudo
     CDS             3868..4271
                     /locus_tag="VWZ30_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4367..5131
                     /locus_tag="VWZ30_001669"
     CDS             4367..5131
                     /locus_tag="VWZ30_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335362.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESYFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKLGAIFIPVAYMMVGGILWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            5131..5883
                     /locus_tag="VWZ30_001670"
     CDS             5131..5883
                     /locus_tag="VWZ30_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335363.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFHLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            5893..6591
                     /locus_tag="VWZ30_001671"
     CDS             5893..6591
                     /locus_tag="VWZ30_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335364.1"
                     /translation="MGKLSSHMWRLNQIIYAKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLVVVYRRRASDFFLEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLSLLCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            6588..7235
                     /locus_tag="VWZ30_001672"
     CDS             6588..7235
                     /locus_tag="VWZ30_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9335365.1"
                     /translation="MNEIITLKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(7322..7702)
                     /locus_tag="VWZ30_001673"
                     /pseudo
     CDS             complement(7322..7702)
                     /locus_tag="VWZ30_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765259.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
     gene            7887..9377
                     /gene="lysS"
                     /locus_tag="VWZ30_001674"
     CDS             7887..9377
                     /gene="lysS"
                     /locus_tag="VWZ30_001674"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU9335366.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            10001..11137
                     /gene="lctO"
                     /locus_tag="VWZ30_001675"
     CDS             10001..11137
                     /gene="lctO"
                     /locus_tag="VWZ30_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEU9335367.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
BASE COUNT         3380 a         1888 c         2305 g         3685 t
ORIGIN      
        1 aatagatgtt aagtaaaaat gtagaatata aaggaggggc aatgagtatg attgaagtta
       61 gccatttatc aaaaagtttt ggtgataaaa tagctttaaa tgatataagt ttcactgtta
      121 aagaaggtca gatttttgga tttttaggac catctggttc tggaaagacc acaacgatta
      181 atattctgac tgggcagctc cttgccgata aaggacaatc tattattttg ggacaaaaat
      241 ctcaaaattt aacaagcggt gaattaaaga gaattggatt ggttagcgat acaagtggat
      301 tttatgagaa aatgtctctg tataacaatc ttctttttta tagtaaattt tataatatta
      361 gtaaatcacg tgttgataat ttgttaaagc gagtaggatt atatgatagt cgcaagatgg
      421 tagcaggaaa attatccact ggaatgaggc aacgaatgct tttagcacga gctcttatca
      481 acaaacccgc tgtactcttt ctggatgaac cgacctcagg tctagatccc acaacttctc
      541 gaacaattca tgagttaatt ttagaattga aaacagcagg gacaacgatt tttctaacga
      601 ctcatgatat gaatgaagca agtcttttat gtgattatgt tgccttatta aataaaggga
      661 aattagttga gcaaggagct ccttctgaac tcattcaaag atataataaa gataaaaaga
      721 ttaaggttac agattataat gggaatcaga taacttttga ttttacatca ctagaacagg
      781 tatctcaggc tgatctggaa aatatttttt caattcattc atgtgagcct actttagaag
      841 atatttttat cacattaaca ggaggaaagc taaatgctta aacggtttct ggctttggta
      901 tggttgcgtt gtcaaatcat cctttccaat aagagtattt tattgcaagt tttagtgcct
      961 tttgctttca catattttta taaatatctt atggaaacac aggggaaggt caacgatcaa
     1021 caggcattag ttcttttgat gatgtgttta cctttttctt tggctttggc tgttggaagt
     1081 cctataacta ttatcttgtc tgaagagaaa gaaaagtaca atttacaaac tcttctgttg
     1141 agtggtgtta aaggctccga atacatttta tcaactatgt ttcttccttt tttgctaact
     1201 tttgtgatta tgggaactac tcctcttatt ttaggagtta caattgtaca tacttttaat
     1261 tatattacaa tcgttcttct aacctcttta tccatcattt tattctattt attgataggt
     1321 ttaaccgcga agagccaagt agtagctcag gttatcagtc ttcctgctat gattttagtt
     1381 gctttcttac cgatgctatc tggtttggat aagacagttg cgaagataac agattatagt
     1441 tttatgggac tatttactaa gtttttcaca aaatgggagg aattttcatg gaataaaact
     1501 ctaattccta atctaacact acttatttgg attgttcttc tattaacttt aattacgata
     1561 actattagga aaaagaaaat ttcttaattg agttatttta atgattataa acacaagtgg
     1621 gaaggaaaaa atgaactgat ctttttgaca gcaattctac agaatagtct tattgctata
     1681 ttttgatttg agtgtacgaa aaaagaaaaa taacaatagt gctcatacta attgcagaag
     1741 ttttgggtga taagataact gataaattgc aataaaaaat gcaacatttt taaatctcct
     1801 ctataagtgc ttcaaaacct gtcttgtaat ccaagtattt ttggggacgg tgattaataa
     1861 gctagcaaag catcattaag gattttttcg gtaattgttg ccaaatcggt ttaagaaaat
     1921 actcacgaag aagtccattc gcattctcat tacttcccct ttgccaagat gaataggcat
     1981 ccgcaaaata aaacagaatt cccatttgtt caattaaagg gtaacaagca aactcttttt
     2041 ctctgtccga agtgaaagtc tttaactatt cttttggaaa gagtcttgtg aggtgttcaa
     2101 tagcagtcaa catggattta gctgttttta cttgacaagt gctagtagaa ataatagaat
     2161 agtaaaaaac ctttaaagca gtccagagag gcagctaagg ttagacggtg aaagggtgga
     2221 gactacccat ttttcgtgga accttgctgt tggcaggttc cttttttcgt ggcttctgtt
     2281 ggccagactc tctcactagt aaaggcaaaa ggagaaacct atgcgagaac atcgtccaat
     2341 cattgctctt gattttccta gttttgaggc ggtcaaggaa tttttagctc ttttcccagc
     2401 agaagaaagc ctttatctca aggtagggat ggagctttat tacgcagcgg ggcctgagat
     2461 tgtgtcctac ttaaaaggtt tgggtcatag tgtctttttg gatctcaaac ttcatgacat
     2521 tcctaataca gtcaagtcag ccatgaaggt cttgtctcag cttggtgtcg atatgactaa
     2581 tgtccatgcg gctggtggtg tagagatgat gaaggcggcg cgtgaaggtc ttgggagtca
     2641 agccaaattg atcgctgtaa cgcagctcac atcaacgtca gaagctcaga tgcaggagtt
     2701 tcaaaatatc caaaccagtc tgcaagagtc tgtgattcac tatgccaaga agacagctga
     2761 agctggcttg gatggtgttg tttgctcggc tcaggaagta caagtcatca agcaggctac
     2821 caatccagat tttatctgtc tgacaccagg gattcgtcca gctggtgttg cagttggaga
     2881 tcaaaaacga gtcatgacac ctgctgatgc ctatcaaatc ggcagtgact atatcgtagt
     2941 gggacgtccc attacccaag ctgaggatcc tgttgcagct tatcatgcca tcaaggatga
     3001 atggacacag gactggaatt aaagaactag attagaaaaa taaaaggaga ataccatgac
     3061 acttgctaaa gatatcgcta gccacctctt gaaaatccaa gccgtttacc tcaaaccaga
     3121 ggaacccttc acttgggcat ctggtatcaa gtcaccgatt tacactgata atcgtgtgac
     3181 actagcctat ccagaaactc gtaccctaat tgaaaatggt tttgtggaag ctatcaaaga
     3241 agcctttcct gaagtagaag tgattgcagg aactgcaaca gcagggattc cacacggagc
     3301 cattattgct gataagatgg acttgccttt tgcctacatc cgtagcaaac caaaagacca
     3361 cggagctggt aatcaaatcg aaggtcgcgt agctcaaggt caaaaaatgg tagtggttga
     3421 agaccttatt tcaacgggtg gttcagttct tgaagctgta gcagcagcca agcgagaagg
     3481 agcagatgtg cttggagttg tagcgatttt cagctaccaa ttgccaaaag cagataagaa
     3541 ctttgcagat gctggtgtta aacttgtgac gctttcaaac tatagcgagc ttatccatct
     3601 agcccaagaa gaaggttaca tcacgccaga gggccttgat cttctaaaac gctttaaaga
     3661 agaccaagaa aattggcaag aaggttaggt cagtaagata aagagagacg aggctaccga
     3721 gcctctttta ccatttcatt taaaatatga caatatttta aatgaaataa ttgacaacgc
     3781 tttcttttat tgatataatg aaaatgtaat atgtatcaaa ttgatcaatc aactatcttg
     3841 tttatcactt cttaggagga cgatgtcatg aaaagaagaa aaatatattc cttttttgcg
     3901 tagttacaat tgcatctagt ttgtttctcc ctacagtttt agctagacag attggaaatt
     3961 atgatttaac gttgccgcgt tggggttcgg atacaactag tgagcttgag aaagaaaacg
     4021 cctctgctgg aattaataat agtgacagca ctggtggcgg taaaaggtta aatacctcta
     4081 ttcgtagcgc ctatagtggg tcagatatta ccccggtata ttcattgggg tctggctcta
     4141 ggattgtcat gtactataat ggaggtggtg acaattatat tggttctggt actagattag
     4201 ctatggcgcc acaatttgga aatcatgtaa gaattcatac ttcaggttct tggagtccag
     4261 attcttatta acttacttgt cagagtaagc cttaaagatg gttgattgtg ggtgtagcat
     4321 gaaaaaagaa tgctacaccc tatttttatt ataaggagga gtaaggatgg aatttttcat
     4381 ttgtaatctt gtacgagtcg ttcaatcacc tcggttttat atgtctttat ttttgaccct
     4441 tctttgcatg agtttaggaa atttccttgc tttcaatggt atttataaaa ttgaaggttt
     4501 atcgattttt tttgccgctt cttctattcg aggattttca ccgattagcc tagtagctgc
     4561 acttatctgt acactgccct attctagtca gataatagag gatgctgaga gttattttct
     4621 aacagcacaa ttgtgccgaa tttctaaaaa gaggtatcta gctattgtag gtagtactgc
     4681 aattatttct tcttttctag tcttttttct cccctattta ttattattag gaattaatct
     4741 tttagtgact ccttatcagg aaatttatat tggagattat agtggtgcct taaaagaatt
     4801 atttgattct aatcagtttc tctatagtct tgtgacgact ctctggtatg gagtttgggg
     4861 cgctgtgttc tctatttttg gactagctag tgctttgcta gtgaagaaaa aattaggagc
     4921 tattttcatc ccagttgcct atatgatggt tggtggtatt ttatgggcta ttttagggct
     4981 atcttactta gaacctgtga caacgctagc tttgggatat cagaaagata tcagtctttc
     5041 cttagttagt gggcatcttg cttttatttt atttgttagt tgtttggttg tttatggtac
     5101 attttttcta cattcagagg actatgtata atgaaacaat ttgttcaatt ttataaaaaa
     5161 gatttcttag cagtattggt ttattttata ttactgctat cctgtgtttt atctagtaca
     5221 gtatatttat tgcgctgtcg ccaatattca atccatccaa atgtattaga atggatctta
     5281 gttttacttc aagatatgac gactggagta tattgctttc cgttcacata tatattgttc
     5341 ttttttcatt tgatgaataa ctattttaat aggttggagt gtcgcattcg tctgaaatca
     5401 attaagcact ttaccagttt tagtttcaaa ttagcagctc ttagtacggg gatttggacg
     5461 gcgactttat ttttattgat ttttctaatt gcatttagta atggttttag cttctctttg
     5521 gagataaagg aggttgattt tttaagagaa ttttatggta taagtattgc aaacaatgct
     5581 agtttcttta taggattttt tttctcttat atagcatact atttcttttt atccttactt
     5641 actattagca gtttttcttg gtttaaaaaa tcaaacatga gcttagtatt tctgtttact
     5701 tttttatttg tagaatcctt attctggatt tatcagttga acaatgggat aattggatta
     5761 ttgccaattt ttcagtatat ggtaaattcc aatccgtatg cattgattta ttggcttaca
     5821 ttactatcta tcataattcc attgactgta ttttctgttc atagaaactg gaggagagtg
     5881 taaaagttgg aaatgggaaa gttaagtagt cacatgtgga ggttgaatca gataatctat
     5941 gccaagtact tttggggtta tgttcttttt tggatattga tttgtttagg attatggtat
     6001 tggttagaag gaaatgatag acttgttata gaaattttaa aagggcctaa tctgagtcaa
     6061 aactcttttt tagtcttatc tatatggttg cttcattggt ttattattca tacatttttt
     6121 ctagtagttg tatatcgtag aagagcatcc gatttctttc tggaagtgat tcgattttct
     6181 tctattaagc tctggattag gtatcagatt tggacctgtt ttctttatgg acttatttta
     6241 atcatggtaa aagttctagt gattcaattt atgttacagt taccaaactg ggatatagga
     6301 gttttgttta tagttgattc tttgaatgct tgtgtgctat ccttgctttg ctttatgtta
     6361 tacgcactag gagcgaatgt acaaatgaac tttgcttgcg ttagtttctt tttactcatg
     6421 attgtgtttg gtggtctatt tgtaggaaat cgaactaatt atctatttta tattttgaat
     6481 cgagggaatg gagatatagg acgagattta tttcttcagt tgctattttt agtctttctt
     6541 tttaaaagca ttttctattt cactcgtcaa aagaggaggt ttatagaatg aatgaaataa
     6601 ttacattaaa aaatattgag ttgaaattaa aaaaaacatg tgtttttcaa aaccttaact
     6661 ttagttgtaa acagggggaa attataggaa ttactggtgc gaatggctca gggaaaagtg
     6721 tattgtttaa attaatagct ggtttatata gtccgtctta tggagaagtg ttaatcaatg
     6781 gggaaaatat tgttcctgag agaaaaattc cagctaattt gggagctttg attgaagaac
     6841 ctggttttat aaattattat agtggcttta agaatttaca atatttggca agcatacgag
     6901 gagtagttgg taatcaggaa atcaatgata cactgaaaat agttggtcta tatgagcaaa
     6961 aagaccagaa agttaaaact tattcgctag gtatgaggaa aaagctaggg attgctcaag
     7021 caattatgga gaatccctct attcttttac tagatgaacc tatgaatgcc ttggataaat
     7081 caagtgtaga aaatatgaga acattgttta gaaagctctc tagtgaaaaa gggacaacaa
     7141 ttttgattgc tagtcatagt gaagaggata ttcgtatctt atgtgataaa gtatatgcaa
     7201 tagaagataa agtatgtaca ctgtgttcag attgaacatc ttagatagac tcaggatgtt
     7261 ataggtaaaa tcttaaaaag ggattggagc ttaaaatcca gcccctttta agagttaatg
     7321 actagtcaac ttttccgcct tcaacaacta ggtcatctgc cttagcagcg tcaccgtagg
     7381 ttgggtggag tgttttttca taggccttgt ggaagaagtt ttccttacct aatttttcaa
     7441 tatctttatt gatgaagtct agcaattctt ggttgccttt ttgaactgct gccgcaatgg
     7501 tatctggatt accgagggaa gtaattccta cttcaaatcc tttattttca agcgcccaag
     7561 ctagaacttc agtattgtca gtagagaagg catctccacg tccgtcaaga agagcttggt
     7621 aagagtcact gtattggtca tatttttgga gtttaatttc tggatgattc ttttcaaaat
     7681 aagtctcagc agtcgttcct tttctgagat tttggaattt cttgcttatc ccagtctttt
     7741 cagtgatttg gctcagtgat cttggcgtca aattcttttt agttttgaaa gattatccgt
     7801 gaatttcttg cttatttatg ataaaatggg agtatcgcaa aaaatgactc atcgtattca
     7861 attttgagta aaactaggag gatcccatgt ctacagaaca tatggaagaa ctaaatgacc
     7921 agcagatcgt tcgccgtgaa aaaatggctg cgctccgcga acaaggaatc gatcctttcg
     7981 gaaaacgttt tgaacgtact gcaaattcac aagaattaaa agataaatat gccaacctcg
     8041 ataaagaaca attacacgat aaaaacgaaa cagctactat cgcaggacgc ttggtaacca
     8101 aacgtggtaa aggaaaagtt ggttttgccc accttcaaga ccgcgaaggc cagattcaga
     8161 tctacgttcg taaggatgct gtcggtgaag aaaactacga aatcttcaaa aaagcagacc
     8221 ttggtgactt ccttggtgtc gaaggtgaag tgatgcgtac ggatatggga gaactctcta
     8281 tcaaggcaac ccacatcaca cacttgtcta aggctcttcg tcctcttcct gagaaattcc
     8341 atggtttgac agacgttgaa acaatttacc gtaaacgtta ccttgacttg atttctaacc
     8401 gtgaaagctt tgaacgcttt gtcactcgtt caaaaatcat ctctgaaatc cgtcgttacc
     8461 ttgaccaaaa aggattcctt gaagtggaaa cacctgttct tcataatgaa gccggtggtg
     8521 ctgctgcccg tccatttatc acccaccaca atgcccaaaa cattgacatg gtgcttcgta
     8581 tcgcgactga gcttcactta aaacgcctta tcgtgggtgg tatggaacgt gtctatgaaa
     8641 ttggccgtat cttccgtaac gaaggaatgg acgctactca taaccctgag tttacttcta
     8701 tcgaagttta ccaagcttat gcagacttcc aagacatcat ggacttgacg gaaggcatta
     8761 tccaacacgc tgctaaatca gttaagggcg atggcccagt caactaccaa ggtactgaaa
     8821 tcaagattaa cgaaccattt aagcgtgttc atatggtgga tgctatcaga gaaattactg
     8881 gtgtcgattt ctggcaagac atgactttag aagaagctaa agctatcgct gctgagaaga
     8941 aagttccagt tgagaaacac tacactgagg ttggtcacat catcaatgcc ttctttgaag
     9001 agtttgttga agaaacttta atccaaccaa cctttgtcta tggacatcca gtagctgtat
     9061 ctccactcgc taagaaaaat cctgaagacc aacgctttac tgaccgtttc gagctcttta
     9121 tcatgactaa ggagtacggt aatgccttta ctgaactcaa cgacccaatc gaccaactta
     9181 gccgttttga agcccaagct aaagccaaag aacttggtga tgatgaagcg acaggaatcg
     9241 actatgacta cattgaagct cttgaatacg gtatgccacc aacaggtggt ttgggaatcg
     9301 gtatcgaccg tctctgcatg ctcctcactg atacaacaac tatccgtgat gtattgctct
     9361 tcccaacaat gaaataaatt cttatcctct ggctcttacc agaggatttt ttgattcaaa
     9421 aagagactga atttaaggag aaaatgaagt gtagtatatt gaagttgaaa tagtacactt
     9481 tgatttctaa gacattgtta gaaattggtt taaattccct aagcaatttg tgcatgtttt
     9541 atttcatttt acgatagtac gctgaaactg ttcaaaaagt actagaaatt gacttggatt
     9601 ccccaattga tttgttcaga ttcactataa ataaaaaatt aataagtggg ataggaagtt
     9661 agcgtcaact aggatagtat cttgcttaaa cagtatatat gggactgata taagtccata
     9721 ggtcctatta gaggatgttc tggtgtctta ttcacttgtt ttttatagta ttagtagata
     9781 gaatcagcaa ataaaaaagt agttcaaatg cctggcagca aaaaatctca acgattcact
     9841 taatatagac taaagtttag agtttaagga aagtagggct agtatattgt atgaaaaaat
     9901 aagatggttt ttaagcaaaa ctcttgaaaa tgattgaaaa aagagttatt atagttgtgt
     9961 agtaaaaagc aaacgcttac ttaatcaagg agggcatttt atgtcataca aaacaagcaa
    10021 tgcagaaggt catgtagact tcatcaatac ctatgatttg gagccaatgg cgcaacaagt
    10081 tattcctaaa gcagcatttg gctatatcgc tagtggggcg gaagatactt tcactttaag
    10141 agagaatatc cgcgccttta accacaaact catcgttcct catacacttt gcgatgttga
    10201 aaatccaagt acagagattg aatttgcagg tgaaaaacta tcttcaccaa tcattatggc
    10261 gcctgttgcg gctcataaat tggcaaatga acagggggaa gtggcgactg cgcgtggtgt
    10321 gcatgagttt ggttctcttt atacaaccag ctcttactct actgttgacc ttccagagat
    10381 ttctgaagcc cttcaaggga caccccattg gttccaattt tactttagta aggatgacgg
    10441 tatcaaccgc cacatcatgg accgtgtgaa ggctgaaggt tataaagcga ttgtcttgac
    10501 ggcagatgct actgtagggg ggaatcgtga agtggataag cgtaatggtt ttgtcttccc
    10561 agttggcatg ccgattgttg aagaatacct gccagaaggt gctggtaaat caatggactt
    10621 tgtttacaaa tcagctaaac aacgcttgtc tccacgcgat gtagaattta tcgctgaata
    10681 ctctggtctt cctgtgtatg tcaagggacc acaatgccgt gaggacgttg aacgttcact
    10741 tgctgcagga gcttctggta tctgggtaac caaccacggt ggtcgtcaaa tcgacggtgg
    10801 accagctgcc tttgactcgc ttcaagaagt ggcagaagca gttgatagac gtgtgccgat
    10861 tgtctttgac tctggtgttc gtcgtggtca acacgtcttt aaagccttgg cttcaggagc
    10921 agacttggta gctattggtc gccctgttat ttatggcttg gctctcggtg gtagtgtcgg
    10981 tgtgcgtcaa gtctttgaac acttgaatgc ggaattgaag acagttatgc aattgtctgg
    11041 agctcagacc attgaagatg tcaaacactt taagctccgt cacaatccat acaacccaac
    11101 cttcccagtt gaccctcgtg acttaaaatt gtattgataa aacagattgc cttcactgaa
    11161 tgtggaggtt tttcttcgtg tattggaaga ataaatcgct atcgaaaact atagtaaaat
    11221 gaaataagaa taggacaaat caatcaggac agttaaat
//
LOCUS       DAVKDZ010000067        10945 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000067 DAVKDZ010000000
VERSION     DAVKDZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10945
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.068"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            29..619
                     /locus_tag="VWZ30_001676"
     CDS             29..619
                     /locus_tag="VWZ30_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEU9335368.1"
                     /translation="MVDKREKLMNSFNQYGFLTFKQVIDENLHYKTLLKMVAEGKIDA
                     EEKGLYRLPDIYLDEWFVLQYRFPKGIFSLETALWLHGLSLTIPFNMTMSFPYGTNTK
                     NIKEADICPIILRSHYSEGIIEIERLPGQFIKVYEVERVLVECLRPVHQVDLQIIAPA
                     FKKYFQQNKIHLHKLFYYAQLFKVTDKLQSYTEVLS"
     gene            619..1455
                     /locus_tag="VWZ30_001677"
     CDS             619..1455
                     /locus_tag="VWZ30_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEU9335369.1"
                     /translation="MFSNANSFKAKIKNISKDKGIPAQQVQQHYLIEQVLKLISTSSY
                     RDSFIVKGGYLIGQMIGLDKRTTMDLDVTLKGTEMSRENLIHIFEEILCSNTDGFSFS
                     VDKLEPIRQDDEYGGFSLKLNATFDTLKEVVFIDITTGDKITPREITYSMTSIFTNES
                     IKIWTYNLETVLAEKLETIISRGLASTRPRDRYDLFTLYKLRKEEINLEVLKNALENT
                     AEKRKSKDTIYNWEEQVRGIEISDYQKELWIRYQRQFKYAKDISFDNSVQVIREIMQQ
                     IF"
     gene            1738..2037
                     /locus_tag="VWZ30_001678"
     CDS             1738..2037
                     /locus_tag="VWZ30_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335370.1"
                     /translation="MKVVNLYDLKQMGNKGGCTIQLIHHFPFGMGLGHLKKDYIEFKR
                     VGIVDGKAVEVTLREPYSRDLLQVVKSIKQRQKLIAYRYKEGKLLFVKKEASDVL"
     gene            2027..2515
                     /locus_tag="VWZ30_001679"
     CDS             2027..2515
                     /locus_tag="VWZ30_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335371.1"
                     /translation="MSSEQQERMAVQYAERSLLFTVKSLLKILEWSRRQALAQDSAYK
                     IGVQKLEELLQSPYSIDTINLKKDFLDKPIDIEKFKAFLEKEEIPLAIAWQGDSLHFY
                     TKDRSILDNHLDHLLEKMVNDPEKLADFTMDKSLDDAIDEAKSQITFRQEGAVKQKEM
                     VR"
     gene            2515..4392
                     /locus_tag="VWZ30_001680"
     CDS             2515..4392
                     /locus_tag="VWZ30_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEU9335372.1"
                     /translation="MYSGKKFLLFSLLGILLGYLFHRLTLLYDSYTGNTLDKWTHLLM
                     EGQDEVLQSPWNVSFTGKSSAFFLLGFVMMLLVYLYLETGKKQYREGVEYGSARFGTL
                     KEKKLFYGKEFSHDTILAQDVRLTLLDKKPPQYDRNKNIAVIGGSGSGKTFRFVKPNL
                     IQMNSSNIVVDPKDHLAEKTGKLFLEHGYQVKVLDLVNMKNSDGFNPFRYIETENDLN
                     RMLTVYFNNTKGSGSRSDPFWDEASMTLVRALASYLVDFYNPPKTREQLIEESRLSQK
                     EYQNLLKRQKKEVEERKKRGRYPSFAEISKLIKHLSKGENQEKSVLEILFENYAKKYG
                     TENFTMRNWADFQNYKDKTLDSVIAVTTAKFALFNIQSVMDLTKRDTLDMKTWGQEKS
                     MVYLVIPDNDSTFRFLSALFFSTVFQTLTRQADIDFKGQLPLHVRVYLDEFANIGEIP
                     DFAEQTSTVRSRNMSLVPILQNIAQLQGLYKEKEAWKTILGNCDSLVYLGGNDEDTFK
                     FMSGLLGKQTIDVRNTSRSFGQTGSGSLSHQKIARDLMTPDEVGNMKRHECLVRIANM
                     PVFKSKKYNSTKHPNWKYLANQETDERWWNYQINPLNQRQENHLEGLRIRDLTFESSL
                     K"
     gene            4413..4655
                     /locus_tag="VWZ30_001681"
     CDS             4413..4655
                     /locus_tag="VWZ30_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335373.1"
                     /translation="MITHFKGFVYGVDASAMFAQAMSLLQKGLIAVGAFLVVVGIVNL
                     ATNIKDGGPGVRNAILEIVGGVMVGAAGAFVTQISI"
     gene            4672..5526
                     /locus_tag="VWZ30_001682"
     CDS             4672..5526
                     /locus_tag="VWZ30_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL"
                     /protein_id="HEU9335374.1"
                     /translation="MNLSLVSPFVYLASEKISAENLFEGFNVDLQSTVDLIKSLSSYN
                     PTVWTYMSSITKSVMQPLGVAILSVVLILEFSKMAKKIANSGGAMTFEALAPMLISYI
                     MVAVVITNTTVIVEAIIGIASHAIEQVASIVAHGGAKYDTISGLKGSGFIGRMIVGFF
                     ALLIWLVRIVSAAMVNLLVSIRFIQLYLMIPFAPLTIPTFLSDEWKSIGIGYLKNIMV
                     YAVQGVLIFLIVSLVPLFESAGKIAVSNGAGVLQSLAIMFGSLVQAILLIIALVGSQR
                     TDRSILGM"
     gene            5580..5939
                     /locus_tag="VWZ30_001683"
     CDS             5580..5939
                     /locus_tag="VWZ30_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEU9335375.1"
                     /translation="MNTRVFKDISKYQHRAWLGFTTRQIIFVLPAFIVTIIVLGLNLF
                     FWQFGDWFVYGFVFAFTIPLMLFGVYKPNDLYFEHYLKYRLHFELTVPLRTITGKKGP
                     EHEKKIKYIKETKNFND"
     gene            5932..8247
                     /locus_tag="VWZ30_001684"
                     /pseudo
     CDS             5932..8247
                     /locus_tag="VWZ30_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            8259..>10945
                     /locus_tag="VWZ30_001685"
     CDS             8259..>10945
                     /locus_tag="VWZ30_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU9335376.1"
                     /translation="MKDKREIIRARKAFRRSLKDEKKFLKQGKKEVRKQKKDSAVLDE
                     KAWKKEIKEKLEEMREASKERVKQANEDYNHILQNSPPSFLNRKELRDRRLPHARKRL
                     KIAKKQFREAKVEAKEERKESRKERKTNQKFLYGQESKQKSNFFFQGKSLEELKAKKE
                     VKAAKENLKSTKQAYKYKKVSRKAKTFLYVLGREGGELASENEDLEGYRTLQETIRKG
                     KRYSRLSYNLGKASVKTGQATGRFTKKRLTNTKERYHHFKDGKGWKLAKDNPSSFKNR
                     FRKLKKQGLSSVQNIYQKLKAAFSFFTFAAGNPATWIVGGIVFLLLLMMSFFLGFSSA
                     SLIQQDEFELTKAYTHLTWEDAEHTRTNDKGITYYTKVDDVMGYMNFKFHDYELQKPV
                     HLFSSETYKDYLSTLWHDLNDGDDLKSMQDLYETPKYKLSKDDQEEIKELKEEGVYAS
                     MQELDNPFEGQSNEDSLTMTYRYGYYDLDGKPTLQEYILLEAKAHQTIVAPMDAVVSL
                     DGDNIILTNGKGENESRLTLYSIHNGRAIEGTRVLTGDIIGETPDDTGLKVSYQKYKN
                     KKEKLVYVNPQFYFPKVIQLQTTILPAIGQFGGDEFERAKHIYDFLKSQGVSPQAIAA
                     ILGNWSVESSINPKRAEGDYLSPPVGATDSSWDDESWLAIGGPAIYSGAYPNILHRGL
                     GLGQWTDTSDGSTRYTALLNYAHSKNKKWYDLDLQLDFMLHGDSPYYQSWLKDFFKNT
                     GSAANLAQLFLTYWEGNSGDKLLERQTRATEWYYQNEKGFSQTNGGQAKSDPQSLEGV
                     RGDLYDHSVPGGGDGMAYAYGQCTWGVAARMNQLGLKLKGSNGEKISIINTMGNGQDW
                     VATASSLGGETGSTPRAGAIVSFVGGTHGTPADYGHVAFV"
BASE COUNT         3677 a         1786 c         2242 g         3240 t
ORIGIN      
        1 tattttattt ctaagaaagg agaatgatat ggttgataaa agagagaaac tgatgaactc
       61 tttcaatcag tatggttttt taacttttaa acaagtaata gatgaaaatt tacactacaa
      121 aaccttatta aaaatggttg cagaaggaaa aatcgatgct gaagaaaaag ggttatatcg
      181 cttacctgat atttatttag atgagtggtt tgtccttcag tatcgatttc caaagggaat
      241 cttttctttg gagacagcac tttggttaca tggtttatct ttgactatcc cttttaacat
      301 gacgatgagt tttccttatg gtacgaatac caaaaacatt aaggaagcag atatatgtcc
      361 tattatctta cgctctcact atagtgaagg aattattgaa atagagcgcc ttcctggtca
      421 atttattaaa gtttatgaag ttgagcgagt tttggttgaa tgtctaagac cagttcatca
      481 ggtggatctt caaattattg caccagcgtt taaaaaatat tttcaacaaa ataaaattca
      541 tttacacaaa ttattttatt atgcccagct atttaaagta actgataagt tacaatctta
      601 tacggaggta ctatcttaat gttttcaaat gcgaatagct ttaaagcaaa aatcaaaaac
      661 atttcaaaag ataagggaat tccagctcaa caagtacaac aacattattt aattgagcaa
      721 gtgttaaagc tgatttctac tagttcttat agagattcct tcattgtaaa aggagggtat
      781 ctaataggtc aaatgattgg actagataag cgaaccacaa tggatttaga tgtcactctg
      841 aagggaaccg aaatgagcag agaaaatttg attcatatct ttgaagagat tctttgttct
      901 aatactgatg gattttcatt ttcagtagat aagctagaac ctattcgtca agatgatgaa
      961 tatggaggat tttcattaaa attaaatgca acttttgata cattaaaaga ggttgttttt
     1021 attgatatta ctactggtga taaaatcaca ccaagagaaa ttacttattc aatgacttct
     1081 atctttacta atgaaagcat caagatatgg acatataatc tagagactgt acttgctgaa
     1141 aagttagaaa caatcatcag taggggacta gcttcgacgc gtccacgtga tcgatatgat
     1201 cttttcacct tgtataaact tagaaaagaa gagattaatc ttgaagtatt aaaaaatgca
     1261 cttgagaata cggcagaaaa acgtaaaagt aaagatacta tttataattg ggaagaacaa
     1321 gtgagaggaa ttgaaatttc tgattatcag aaagagctat ggattcgtta tcaacgccaa
     1381 tttaagtatg ctaaagatat ctcatttgat aactctgttc aggtcattag ggaaattatg
     1441 caacaaatat tttagtacaa aagtaactca ttagtaaagc agtgagttgc tttttttatt
     1501 ccaataactc caaaaatgtg ataaaaattt caactactct tatctataat aaagctacct
     1561 gttctttgaa aattgaatcc actccgtctt gattagcgtg cctgtgtaag tagggactga
     1621 gagtatttcc ctgtgaaggg caactggcac aagacgtgtg tgagaatttt ctaacagaca
     1681 cggagtttat cgttaccaga cttaaacgat gcgacaaact agataaagga gttaatcatg
     1741 aaggtagtaa acttgtatga tttgaaacaa atgggaaata aaggtggttg taccatccaa
     1801 ttgattcatc actttccttt tggtatgggc ttaggacatc tcaaaaaaga ctacattgaa
     1861 tttaaacgtg ttggtatcgt tgatgggaag gcagtagaag tcactcttcg agaaccttat
     1921 tcgagagatc tactgcaggt tgtcaagtcg attaagcaac gtcagaaatt gatagcttat
     1981 cgttataaag aaggaaagct gctatttgtg aagaaggagg catcagatgt cctctgaaca
     2041 acaggaacga atggcagttc aatatgcgga gcgtagtctt ttatttactg tcaaaagtct
     2101 cttaaaaatt ctagaatggt ctagacgtca ggctttagca caggattccg cctataagat
     2161 aggggtgcag aaattagaag aattgctaca atctccttat tcgattgata cgattaatct
     2221 gaaaaaagat tttttagaca aaccaattga tatagagaaa tttaaagctt ttttagaaaa
     2281 agaagagatt cctttagcca tcgcttggca aggggattct ctacatttct acacgaaaga
     2341 ccgttcgatt ctggacaatc atttagacca tctgttagaa aaaatggtta atgatccgga
     2401 gaaattagct gattttacca tggataagtc attagacgat gcaattgatg aggcgaaatc
     2461 ccaaattacc tttagacaag aaggggccgt caaacagaaa gagatggtga gatgatgtac
     2521 agtggaaaga aattcctact attttcactg ttaggtatct tactaggcta tctttttcat
     2581 cgtttgacgc ttttgtatga ttcctatact ggaaatacat tagataaatg gactcatctt
     2641 ctgatggaag gtcaagatga agttcttcag tcgccatgga atgtttcctt tactggaaaa
     2701 tccagtgctt tttttctact aggctttgtg atgatgttgc tggtttatct ttatttagag
     2761 actggcaaaa aacaataccg agaaggggta gaatacggga gcgcccgttt tggaactcta
     2821 aaagaaaaga agctctttta cggtaaggaa ttttctcatg atacgatctt agctcaagat
     2881 gttcgtttga cattattaga taaaaaacca ccccaatatg atagaaataa gaatattgcg
     2941 gtgatcggag gttcaggaag tgggaagaca tttcgctttg tgaaacctaa tcttattcag
     3001 atgaatagtt ctaatattgt agtggatcct aaagatcact tggccgaaaa aacaggcaaa
     3061 ctctttttag aacatggcta tcaagtaaag gtgctagatt tagtcaatat gaagaactca
     3121 gatggcttca atccttttcg ctatatagag acagaaaatg atttgaatcg catgctgacg
     3181 gtttatttta ataacaccaa aggctctggc tcccgaagtg acccattttg ggatgaagct
     3241 tctatgactt tggttcgagc tttagcctct tacttggtcg atttctataa tccacccaaa
     3301 acaagagaac agctcattga agagagtcgt ttgagtcaaa aagaatacca gaacttgttg
     3361 aaacgtcaaa aaaaagaagt ggaagagcga aaaaaacgag gacgttatcc aagttttgct
     3421 gaaatctcaa aactcattaa acacttatct aagggtgaaa accaagaaaa aagtgtctta
     3481 gaaattctat ttgaaaatta tgctaaaaag tatggcactg aaaattttac catgcgaaat
     3541 tgggcagatt tccaaaatta taaggataag actctggatt ctgttatagc tgtaaccact
     3601 gctaaatttg ccctcttcaa tattcaaagt gtcatggatt tgaccaaaag agataccctt
     3661 gatatgaaga catggggcca ggaaaaatca atggtttact tagttatccc agataacgat
     3721 agtacctttc gctttctttc agccctcttt ttttcaaccg tatttcaaac cctaacaaga
     3781 caagcagata ttgattttaa gggtcaattg cctcttcatg tgagagtcta cttagatgag
     3841 ttcgcaaata taggagaaat cccagatttt gctgaacaaa cctcaacagt ccgttctcga
     3901 aatatgagtc tcgttcccat tcttcaaaat attgcccaac ttcaagggct ctataaagaa
     3961 aaagaagctt ggaaaaccat tttggggaac tgtgatagct tagtctactt aggtgggaat
     4021 gatgaagata cctttaaatt tatgagtggc ttactcggta aacaaaccat tgatgttcga
     4081 aatactagtc gttcctttgg ccagacaggt tcaggatccc tttctcatca aaagattgct
     4141 cgtgatttaa tgacacctga tgaggtcgga aatatgaaac ggcatgaatg cttggttcga
     4201 attgccaata tgcctgtctt taaaagcaaa aaatacaatt ccactaagca tccaaactgg
     4261 aaatacctag ccaatcaaga aaccgatgaa cggtggtgga actatcaaat caatcctttg
     4321 aatcaaagac aagaaaatca tcttgaaggc cttagaattc gtgatttaac ttttgaatct
     4381 agtttaaaat aaaaatagaa aagaggaaat agatgattac gcattttaaa ggttttgttt
     4441 atggagtaga cgcaagtgct atgtttgcac aagctatgtc tttgttacag aagggtttga
     4501 ttgcggttgg tgcctttctc gttgttgtgg ggattgtcaa tcttgcaacc aacattaaag
     4561 atggtggacc aggtgttcgg aatgccattc tggaaattgt cggtggtgtt atggtagggg
     4621 ctgctggagc ctttgtaact caaatttcaa tttaggagga taaacaatga catgaatctt
     4681 agtttagtct caccctttgt ttaccttgca tctgaaaaaa tatcagctga aaatttattt
     4741 gaagggttta atgtagattt acaatctacg gtagatctga ttaaatccct atctagctac
     4801 aatccaacag tttggactta tatgtctagt attactaaaa gtgtcatgca gcctcttgga
     4861 gttgcgattt tatcagttgt tctcatctta gaattttcga agatggcaaa gaaaattgct
     4921 aactcaggag gagcgatgac ctttgaagca ttagcgccga tgttgattag ttatatcatg
     4981 gtcgcagtcg tgattaccaa tactaccgtt attgtagaag ccatcatcgg gattgcgagt
     5041 cacgccattg aacaagtggc ctcaattgtg gctcacggtg gggcaaagta tgataccatc
     5101 tctggattaa aaggttcagg atttattggc cggatgattg tgggcttttt cgccctcctc
     5161 atttggctag ttcggatagt aagtgcagcc atggttaacc ttttggtatc tattcgattt
     5221 attcaactct accttatgat tccatttgcc cctcttacga ttccaacatt tttaagtgat
     5281 gaatggaagt ctattggtat tggctattta aaaaatatta tggtttatgc ggtacaaggg
     5341 gttctcattt ttctgattgt ttctcttgtt cctttatttg aatctgctgg gaaaatagct
     5401 gtttcaaatg gtgcaggagt cttgcaatca cttgcgatta tgtttggtag tttggtacaa
     5461 gctatcttac tgattattgc cctcgttggt tctcaacgta cggatcgctc aatcttaggt
     5521 atgtaattag ataaaggcta ggaagtgatt gcttcttagc ctttttagaa aggaaagtca
     5581 tgaatacacg tgtctttaaa gacatctcaa aataccaaca cagggcttgg ttaggtttca
     5641 ccacaagaca aatcatcttt gttttaccag cctttattgt cacaattatt gttttgggct
     5701 tgaatctctt tttctggcaa tttggagatt ggtttgttta cggttttgtg tttgctttta
     5761 ccatccccct catgcttttt ggagtctata aacccaatga tttatatttt gaacattatt
     5821 tgaaataccg tcttcatttt gaactaacgg ttcccctacg cacaattaca ggaaagaaag
     5881 gacctgaaca tgaaaagaaa atcaaataca ttaaagaaac aaaaaacttc aatgactaat
     5941 cagaaagaag tagttaaagg aaaaaaagag gaagtgttac catcgacggc taatactctt
     6001 tcctatcaag ccctgtatca aaatggtctg atgcaggtaa aagaagatta tttctcacaa
     6061 agctatttac ttggtgatgt caattaccag actgttggtt tagaagataa gggcgcaatc
     6121 attgagaagt attctgattt gattaactcc ctagacgacc aaaccaactt ccaattgact
     6181 atctttaata aaagattgaa tttagaaaag tttagacaaa gtgttttgta tgaggaaaaa
     6241 gaagatgggt atgatagcta tcgtaaagaa ttgaatcgga tgatgaatca gaatttagac
     6301 agtggtgaaa ataacttttc agctgtgaaa ctgattagct ttggtagaaa ggattctaat
     6361 cccaaacaag cctatcgttc cttgtctcaa attggagaat atttcaagag tggtttctca
     6421 gaaattgatg ctcgatttga atctttggct ggagaagaac gggtgaactt gctggcagat
     6481 atgcttagag gagaacacca tcttcctttt tcttaccgtg atttaacgag atctggtcag
     6541 acaactcgtc acttcatagc acctaatctc ttggatttta aaaacaagaa ttacctacaa
     6601 atcaatgacc gcttattgca gattgtctat gtcagagact acggtatgga attaggagat
     6661 cagtttattt gagacctcat gcaaggagat ttggaattga ttgtgagcct ccatgctcaa
     6721 agttcgacca aggcggatgc tatgaagaaa ctacgaacaa agaagacctt gatggaatcc
     6781 caaaagattg gggaacaaca aaaacttgct cgtacaggta tctatttgga aaaagtaggt
     6841 catgtattag aaagcaatat cgatgaagct gaggaactct taaaaaccat gaccgagaca
     6901 ggagataaac tatttcaaac agtattcttg attggtgttt ttggtcagga tgaagaagaa
     6961 cttaaacaag ctctagatac gattcaacaa gtggccggct caaatgacct aatgattgat
     7021 aaactaccat atatgcaaga agcagccttt aatagtttgc tgccatttgg ttgtgatttt
     7081 ttagagggag tatcacggag tttattaaca tccaatatag cagtgaactc tccatggact
     7141 tcagtagact tacaagaccg tagtggaaaa tattacggta tcaatcaaat ctcaagcaat
     7201 attattacca ttgatcgcag cctattaaat acaccgtctg gtctgatttt agggacatct
     7261 ggagctggga aagggatggc aaccaagcat gaaattatca cgacccaaat caaggaatct
     7321 ggtgaaaata ctgaaattat catcgtggat ccagaagcag aatacagtgt cattggacga
     7381 gcttttggtg gagaaatgat tgatattgcg cccgattccc aaacttatct caatgtcctt
     7441 gatttgtctg aggaaaatat ggatgaagat cctgtaaagg taaaatcaga atttctttta
     7501 tcctttatcg gcaagttatt ggatagaaaa atggatggaa gagaaaaatc gattatcgac
     7561 cgagtcacaa gactcaccta tcagtcattt aaagagcctt ctttggaaga atgggtcttt
     7621 gtcttgagcc aacaaccaga agaagaagcg cagaatttgg cacttgatat ggaactgtat
     7681 gtcgaaggtt ctcttgatat tttttctcat aagaccaata ttcagacagg atctaatttc
     7741 ttgatttata acgttaagaa gttaggagat gagctgaaac aaatcgccct tatggttgtt
     7801 tttgatcaga tatggaatcg tgtcgttcgg aatcaaaaat tagggaagaa gacctggatt
     7861 tattttgatg aaatgcagct tctcttatta gataaatatg ccagtgattt cttctttaaa
     7921 ttgtggagtc gtgtcagaaa atatggagcc agtctgactg gaataactca aaacgtcgaa
     7981 accttattgt tagatccaaa tggtagacgg attattgcaa atagtgaatt tatgattctt
     8041 ctcaagcaag caaaaaatga ccgagaagaa ctggttcaac tcttaggctt gtcaaaagaa
     8101 ctcgaaaaat acctagtcaa tccagaaaaa ggagcaggac tgataaaagc tggttcagtt
     8161 gtcgtaccat ttaaaaataa gattcctcaa ggtactcaat tgtttgatat tatgagtacg
     8221 gatcctgata aaatggcttc taattaaggg gaaggtaaat gaaggataaa agagaaatca
     8281 tacgtgcccg aaaagcattt agaagaagtc taaaagatga gaagaaattc ttgaaacaag
     8341 gaaagaagga ggtgaggaaa cagaaaaaag attccgctgt actggatgaa aaagcatgga
     8401 aaaaagagat aaaagaaaag ctagaggaga tgagagaagc ttcaaaggag agagtaaaac
     8461 aagcaaatga agactacaat catattcttc aaaatagtcc tccatctttt ttgaatcgta
     8521 aagaattaag agacagacga ttacctcatg ctaggaaacg attgaaaata gccaagaagc
     8581 aatttagaga agccaaggta gaagcaaaag aagaaagaaa agagagtcgt aaagaaagaa
     8641 aaaccaatca aaaatttctc tacggtcagg aatcgaaaca aaaatctaat tttttctttc
     8701 aagggaagag tttagaagaa ttaaaagcta agaaagaagt caaggctgct aaagaaaatt
     8761 taaaatctac taaacaagcc tataagtaca aaaaagtcag taggaaagcc aaaacttttc
     8821 tttatgtcct tggacgtgaa ggtggagagt tagcttcaga aaatgaagat ttagaaggct
     8881 atcgcacact tcaagagacc attagaaaag ggaaacgcta cagtcggctt tcttataatc
     8941 ttggaaaagc tagtgtcaaa acaggacaag caacaggtcg ttttaccaag aaaagactga
     9001 ccaacacaaa agagcgatac catcatttta aggatggaaa aggatggaaa ctagcaaaag
     9061 ataacccaag ttcctttaag aatcggtttc gaaaattaaa gaaacaaggt ctttcaagtg
     9121 tccaaaatat ctatcaaaaa ctaaaagcag ccttttcctt ctttacattt gcggctggaa
     9181 atcctgcaac atggatagtt ggaggaatag tctttcttct tttacttatg atgagcttct
     9241 tcttaggatt ttcatctgct agtttgattc aacaagatga atttgaatta acaaaagctt
     9301 atacccacct aacttgggaa gatgcagaac atactcgcac aaatgacaaa ggaattacct
     9361 attacacaaa agttgatgat gtgatggggt atatgaactt taaattccat gactatgagt
     9421 tacagaaacc agttcactta tttagttcag aaacttacaa ggattatctg tctactttgt
     9481 ggcatgattt aaacgatggt gatgatttga aatccatgca agacctctat gaaactccta
     9541 agtataaact atcgaaagac gatcaagagg aaataaagga actaaaagaa gaaggtgtgt
     9601 atgcttccat gcaggaatta gacaatccat ttgagggaca aagcaacgaa gatagtctaa
     9661 ccatgactta tcgttatgga tactatgatt tagatgggaa acctactctt caggagtaca
     9721 tcctactaga agcaaaggct caccaaacga ttgtcgcacc aatggatgca gttgtatctc
     9781 tagatggtga caatattatt ctcactaacg gaaaaggaga gaatgagagt cgattaacct
     9841 tgtattctat tcataatggc cgtgcgattg aggggacaag agtcctaacg ggtgatatta
     9901 ttggtgaaac accagacgat acaggtttga aagtttccta tcaaaagtat aagaacaaga
     9961 aagaaaaatt ggtgtatgtc aatccgcaat tttattttcc aaaagtcatt caacttcaga
    10021 ccactatctt acctgccatt ggtcagtttg gtggggatga gtttgaacga gcaaaacata
    10081 tttatgattt tttgaaatct caaggcgtaa gtccccaagc cattgcggct attttaggaa
    10141 attggtctgt agagtcttct attaatccaa aacgagctga aggagattat ttatctcctc
    10201 cagttggcgc taccgattcc tcatgggatg atgaaagctg gttagcgatt ggaggtccag
    10261 ccatttatag tggtgcttat cctaatattc ttcatagagg tttggggtta gggcaatgga
    10321 cggatacttc agatggttca acacgttata cagctttatt gaattatgca catagcaaaa
    10381 ataagaagtg gtatgattta gacctccaac ttgattttat gcttcatggg gatagtcctt
    10441 actaccaaag ttggttaaaa gatttcttta aaaatacggg cagtgcagcc aatctcgccc
    10501 aactcttcct cacctattgg gagggaaatt ctggtgacaa attactggaa agacaaacca
    10561 gagcaacgga atggtattac caaaatgaaa aaggcttcag ccaaacaaat ggaggacagg
    10621 caaaaagtga cccacaatcc cttgaagggg ttcgtgggga cttgtatgat cattctgttc
    10681 ctggtggtgg agatggtatg gcctatgcct atggacaatg tacatggggt gttgcggctc
    10741 gtatgaatca attaggctta aaattaaaag gaagtaatgg agagaagatt tcaatcatta
    10801 ataccatggg aaatggtcag gactgggttg cgacagcttc aagtcttggt ggggaaactg
    10861 gctctacacc aagagcaggt gctattgttt cttttgtggg aggtacacat ggcacaccag
    10921 cagactatgg tcatgtagct tttgt
//
LOCUS       DAVKDZ010000068        10855 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000068 DAVKDZ010000000
VERSION     DAVKDZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10855
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.069"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(34..450)
                     /locus_tag="VWZ30_001686"
     CDS             complement(34..450)
                     /locus_tag="VWZ30_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9335377.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(462..1157)
                     /locus_tag="VWZ30_001687"
     CDS             complement(462..1157)
                     /locus_tag="VWZ30_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9335378.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPQEMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            complement(1351..3852)
                     /locus_tag="VWZ30_001688"
     CDS             complement(1351..3852)
                     /locus_tag="VWZ30_001688"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU9335379.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSTEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLIRSKEIVAENNGALD
                     KVYNETVKAVTEQIDSLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLTAPFAPHL
                     AEELWQTVAATGESISYVTWPTWDESKLIEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            complement(4307..5395)
                     /locus_tag="VWZ30_001689"
     CDS             complement(4307..5395)
                     /locus_tag="VWZ30_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEU9335380.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(5413..6081)
                     /locus_tag="VWZ30_001690"
     CDS             complement(5413..6081)
                     /locus_tag="VWZ30_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU9335381.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEVGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            complement(6100..8538)
                     /locus_tag="VWZ30_001691"
     CDS             complement(6100..8538)
                     /locus_tag="VWZ30_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEU9335382.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELELFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDSASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(8695..9987)
                     /locus_tag="VWZ30_001692"
     CDS             complement(8695..9987)
                     /locus_tag="VWZ30_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9335383.1"
                     /translation="MAKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFETMIPAFVIFLSSMIVYILASSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAAIVYGSIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKIQDRLAYQNEIK"
     gene            complement(10004..10312)
                     /locus_tag="VWZ30_001693"
     CDS             complement(10004..10312)
                     /locus_tag="VWZ30_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9335384.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            complement(10358..10678)
                     /locus_tag="VWZ30_001694"
     CDS             complement(10358..10678)
                     /locus_tag="VWZ30_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9335385.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
BASE COUNT         3324 a         2356 c         1791 g         3384 t
ORIGIN      
        1 ccttaaattt tgcttaagaa aaaacaagtt ttgttatttt ttacgattca tccaagtcat
       61 ccctatacaa ttataggtgg ataagatttc aaagcccata gaacgataga agcccaaggt
      121 tctttctgtc tcttctgtca ccagctggac ttgataggca tctttgtaat cctctaaagc
      181 ctctttcatc aaggcactac caattccttg acgctgatag attggtaaaa cgattaaatc
      241 ctgaaccaat actgatgaga atccatctcc aaccaaacga atcaagccca ccacagcatc
      301 accatcaagt gccagataaa ttactaatga atgagataag gcctgctcca gcatctctgg
      361 ttgatgggta taatttgtcc aaccgacagc ctgatagaga tgtaaaacat cctctagctt
      421 gacaatttct tgctttttaa tagtaatcat ctcaacacct cttaaagttc tctcaagctc
      481 ttgtactgct gtccattttt atcaaaattt tcaggacgca accatgtttc caaacgatct
      541 ttgacttgag gccagtcctt atcaatcata gacaaccaat ccgtatctct cgtacgtccc
      601 ttataaacca ctgcctgacg gaaggttcct tcataaataa agctcaaacg ctccgcagct
      661 cgtctggatg gcagatttaa ggcatcgcat ttccactcgt agcgacgata gtttagctcc
      721 tcaaaaacat agcgagctag gagatactga gcttcagttc ctatccgtgt accccttagc
      781 tctggagaaa aagtgactgc tccgacttct attactcggt tattctggtc aatgcgcatg
      841 agagaaaaag atcccaaagc cttaccagtc gccttgtcta tgattgcata gtaaaaacgg
      901 tccttacgag ccaacatctg atgtaaaagg ctaaccagct cccccatatc tgctactggc
      961 tcctgaaaaa ggtaggtcca catctcctga ggcgtatcag gcccataaac agctagcaaa
     1021 tcctccgcat gcttttccac tgaaagagcc tctatcctag cataacgccc ttctaagaaa
     1081 tcaatagaag gcagttcacc tggtgtataa ccttccattg actccccaat catttgacca
     1141 tattcattta ctggcatagc tttctccttt tttcactttt caaactttat agttgcccat
     1201 agtataccat actttcataa gaagattcaa aaaatcctcc agattctact ctgaaggatt
     1261 cctaccttat ttcacaacga tattaacgag tttagttggc tcaaacagtt taggaaactg
     1321 tttgaggttg gaaatcagca aataaattgg ttattttaca acaatattaa ccaatttatt
     1381 tggtacacta atcactttca cgatttcctt accgtcaatt tctgctttga ctttttcatc
     1441 cgctagagca atttcttgca attcttcacg tgataggtct ttagcgacca tgagtttggc
     1501 acggacttta cctttgattt ggacaacgat ttcgatttcg tcttcaatca gtttactttc
     1561 gtcccatgtt ggccaagtca cgtaagagat tgactcacct gttgcagcaa cagtttgcca
     1621 gagttcttct gccaagtgag gtgcaaatgg ggcagtcaat tggataaatc ctttggcata
     1681 gtcaacatag agcttgtctt ccttgttagc cgcattgaca aagaccataa gttgggcaat
     1741 ggctgtgttg aatttgaggg aatcaatttg ctctgtgaca gctttaacag tttcgttgta
     1801 aaccttgtca agagcgccat tgttttccgc aacaatttcc ttacttctaa tcaaacggta
     1861 aacacggtca aggaacttac gacttccttc cagaccttct tctgaccaag caatcgaagc
     1921 atcgagtggt cccatgaaca tttcatagac acgaagggta tcagcaccgt attgttccac
     1981 cacatcgtct gggttgacaa cgttcttgag cgatttagac atcttggctg gcgcttgctc
     2041 caactcttcc cctgtttcca catggaagaa agaaccatca cgtttttcaa ccttgtcagt
     2101 cgccacaaga gccccacggt ggtcacgata gcttgttccc aaaatcattc cttggttaaa
     2161 gagtttttgg aatggttcct tagttggaac aacaccgagg tcatagagga atttatgcca
     2221 gaaacgagcg taaagcaagt gaagtacagc atgttccgca ccacccacgt agatatctac
     2281 tggcaaccat tgtttgagca gatcctcgtc agccaatttc tcagtattgt gtgggtcaat
     2341 atagcggagg tagtaccagc ttgaacctgc ccattgtggc atagtatttg tttcacgacg
     2401 acctttgaca ccatcttcac gagtcacttc aagccagtct gtcaagttag ctagtggact
     2461 ttcaccagta cctgagggac ggatatcctt ggttacaggc aagacaagtg gcaattcact
     2521 ttcagggaca gctgttgaag ttccatcttc ccaatgaatg attgggattg gctcacccca
     2581 gtaacgttga cggctaaaga gccagtcgcg gagacggtag gttaccttct cttgtccaca
     2641 gcctttttct tccaaccaag ccacaatctt ggcaatagcg tcttctttgt tgagtccatc
     2701 taggaagtct gaattaacgt gaaggccatc ttctgtgtag gcagcttctt caacatttcc
     2761 accttcaagt acttcgacga ttggaaggtc aaattgtttg gcaaattccc agtcacgttg
     2821 atcgtgggca ggcacagcca ttaccgcacc tgtaccatag ctagcaagaa cgtaatccgc
     2881 aatccagatt ggaatttcct taccattgac aggattgatg gcataagcac cagtccatac
     2941 accagttttt tctttggcaa ggtcggtacg agccaagtct gacttaaggc tggcttggtg
     3001 tttgtagtct gctactgctt ctgcttgctc tgtgcttgtg atggcatcta ctaattcatg
     3061 ttcaggagcc aaaacagtga aagtcgcacc gaaaagtgtg tccggacgag tagtaaagac
     3121 tgtaaattcc ttgtctgttc cttttacttt gaaagttaca ttggcaccag ttgatttacc
     3181 aatccagttg cgttgcatat ccttgataga ctctgaccaa tctagttcat ctaagtcatt
     3241 gagcaagcgc tctgcgtaag ccgtgatttt gagcatccat tggcgcattg gtttgcggac
     3301 aactggatag cctccacgtt cagaagttcc gtcaggaagc acttcttcat tggcaatggc
     3361 agttcccaat tcctcaaccc agtttactgg cacttcagct tcataggcca agcctttttc
     3421 gtaaagcttg gtgaaaatcc attgcgtcca cttgtagtag tttggatctg ttgtgttaac
     3481 ttcacgatcc cagtcataag aaaatccaag cgcattgatt tgacgtttga agttggcaat
     3541 gttttccgct gtaaattctg ctgggtcatt accagtatcc atagcgtatt gctctgcagg
     3601 caaaccaaaa gcatcccaac ccattgggtg aaggacattg tagccttgcg cacgtttgta
     3661 acgactgagg atatcggttg cagtataacc ttctgggtgt cctacgtgca gaccagctcc
     3721 agacggataa gggaacatat caagcgcata aaacttaggt tttgatgcat ctgttcctgt
     3781 cttaaatgta tgatgttctg cccagtagcc ctgccactta ggctcaattt ctttatgatt
     3841 gtaaaaactc atggtctctc tccaattttg tgatgttact attataccat ttttgaggga
     3901 atttgaaatg tgtttaaagg aaaaactgta tactgtatct cattaatttt gatgactatg
     3961 aattgctcta tctcccactt tttatcccat tgttatgcag taaatcaagg agtaagtcaa
     4021 gacgcttatc tcgtatagca caaaatactt attttagacc gaagataaaa ctggaatgct
     4081 aattaatgta ttcacaataa atagccgaca acctatgtcg ctttttgatt tatgaagaac
     4141 gtatagtgga ctgaatctga aatagtacga aacaattgct aaaacattgt tagaaattaa
     4201 ttttactttc ccaatcgatt tgttctcatc ttatttcaat ctgctataga attcaatacg
     4261 aggatcaata aaagactatc atttggaaaa cagtagtcct tattgtttat tttgaattta
     4321 cataggcatc tacagcgata atagcttgag caacatctga aggcgaaatc ttaaacggca
     4381 tctgatgaat tgtctcaccc tccatagttg cttgtttacc aacttttatt aaatcatcat
     4441 atccaacttg atccaaatgc atttctttta gagttgttgg cataccaatt tttttgtaaa
     4501 actcaatata cttatcaagt tcttctttag gtctattttc caataatagt tgtactaaag
     4561 ttccataagc tactttttca ccatgtgtta aatgatgaat gtcacctgtc aatgcagtaa
     4621 aaccattatg aattgcatgc gccgcagcta atcctccact ttcaaaacct aggccactca
     4681 ataaagtatt agcttcaaca atattttcta atgctggtgt caccacttta gcttcacaag
     4741 ctgccatagc ctgtaaacca tctgcaaaca gcgtttcttc acatttcttc gcaattgcaa
     4801 ctccagccaa tgtttgctgt tgtcccaaca tagtttttcc atttgcctgc ataaccgcac
     4861 gcgcctcaac ccaagttgct aaaccatctg caataccaga cgctaataaa cgcttagggg
     4921 cttgtgaaat aacttttgta tcaaccaaaa ctaaatctgg atttttagaa taaaatagat
     4981 aatgatcaaa tgcaccttca tctgtataaa taacagataa agcagataca ggtgcgtcgg
     5041 tcgatgcaat tgttggagca ataataacag gcttttcaat caaatctgca atagcttttg
     5101 cgctatcaat cgtctttccc ccaccaagac cgataatact atcacaattt tctttctcag
     5161 ccaaggcaac aactcgattg atttcattgt cagaagcttc accattaaat agcgccagaa
     5221 caatatggaa accataccta tgtaggtaat cttcaaatcg ttttccaaca atatcataaa
     5281 ccaactgatc gcataataga atagggcaat ttcccaaatc caaaattgat ttggcatttt
     5341 caaacaaggc attttccccc tgaatatatc tagaaggact agcaaaaatt ctcatatgta
     5401 tttcctctct atctaaatgg aacgactatt ttgaataaca aaccagtcgt cagaaaagtc
     5461 atcaaccgcc ttttggatag atggcatggc gaaagctgat tcaaaaacat ctgctcctgc
     5521 tgtaacagca tgcgcacctg cagctaaagc attatttact tgtgctacat ttttaaagga
     5581 tgcagctaaa atcttactag gagagttctg tctatcaata gcaagagcta attgacgaat
     5641 gacagaattt gaatcaatgt tcagattttc cattctatta taatatggag ctaggtaatc
     5701 cgctcctact tcgatagcta ataatccctg aataactgta taaatagctg ttgcagtgat
     5761 atggtagccc tcttttttta gcacctttat tgcacgtaat ccagctggag taacaggtac
     5821 tttgataaat atatcatctc ctgcttgtct tcgaatttca tgagcatcct ttaagatgcc
     5881 ttcaaaatct tgagaaatca cctgaacatg aatagagggt gtagagccaa tcaattctct
     5941 tacatctttg attcgttcaa aaaaattaat agaaccctct ctttttgcaa tagtgggatt
     6001 tgaagttacc ccagctagcg gcaaaatttc agaccacttt ttaatctcat ctaaatttaa
     6061 tgtgtcaagc ataaattcca taaaaagaac ctctttattt tacaaagtat gctcagtacg
     6121 tcctataatg tcatcttggg ttgccttaga aagaacattg aagaatgcag agtatcctgc
     6181 aacacgaaca attaagtctc tgtgtttttc aggatgtttc tgagcgtcaa tcaacgtctc
     6241 tctggaaaca acattgtatt gaatatggta cccatgtaaa cgattaaaga atgttcgtaa
     6301 caaagcaatt agttttaatt tatcttcttc tttggctaac gtttgaggat ttactttctg
     6361 atttaagaga accccaccta cgatttcatc tgttggtaat tttgaaacag attttaaaac
     6421 agatgtaggg ccgtgttgat ccatattatg tgatggtgaa caaccctctg ctaacggtgt
     6481 acccgcgttg cgtccatctg gagttgctaa tgttccacgt ccctgcccta cgttggctga
     6541 tatagaagat gttcctgaat aacgaattcc tccaataggc cctcttccat aacgtgtatt
     6601 aggatattta gcaatttcat caacataaat gtcataagca gcagtaacca atttgtcagc
     6661 ataatcatca tcattaccat acttaggtgc atcatgaatc aacatttctt gaatgacctt
     6721 accttcttct ccggcataat ctgtttccag tgcatgccaa agctgacttg ggcttatacg
     6781 ttcttcctca aacaccaatt ttttaattgc agctaatgaa tccgacaaat ttgcaattcc
     6841 aacttgcaat cctgatatat aatcatatac tgctccacct tctttaaggt gttttccacg
     6901 accaatacaa tcatcagtca atgctgaaca tagaatatca ggaacttctc gttccaatga
     6961 taaatcaata gaattttcaa caataacact cattcgtgtc aaatatctta gtgttttatc
     7021 ccaagcattt tctaattcag aaaagttctt catatcctta aaatgaccaa agcttggtgc
     7081 aaaccgttta cccgaagccg gatcaattcc atcattcatc gtgataagta gaaccttagg
     7141 gaagttcata taactcatac ctgtgcaacg atagccccat ttccctggaa ctgccgtttc
     7201 aacacatcca atggcactgt aatcataagc atcatcttcc aatactcctt ttgcaataaa
     7261 agaaggaata ataatctcat cattattaaa tgcaggcata ccaaaaccaa gtttcatcac
     7321 ttcaatacac tcattcatga aacgagcatc taaacctgca tggtaacgta cagttagatt
     7381 aggttgcggt agatgggttt gtgcaactga ttttaatacc aaataagata atgggttaac
     7441 agcatccttc ttatctcgag tctgtccacc aattgtaaca ttttgatata aaggacttcc
     7501 tgctgaagaa aatgtatgtg attgactgcg aaccttattg attgtaattg tcttaatcca
     7561 aagatttgtc agacgttcaa caatgctatc ttctgtttct ttaccacttt ctaaatcagc
     7621 cttcatatat ggatacatat attgatcaaa acggccatat gaaagagagt ggccattaga
     7681 ttcaatttgt aaaatacatt gaataaacca aactgattga atagcttctg caaaagtagt
     7741 tgccggttca tatgggactc tagagcaaat atctgcaatc tcaagtaatt ctttcttacg
     7801 tttaggattt gcattttcgg ctaaactttt agcaagagca acaaagcgct ttgcatatac
     7861 tttaatagca tcgattacga taaatataga gtcgtaaaaa tgatatttat caatacttgc
     7921 tgaatctgtt aaatctagag ctacttttgc tttacgagcc cgctcttcaa aacctcttaa
     7981 accaaattgc aaaagtttct gatagttaac tgctaagtga gcatctccag aattcatctt
     8041 accttccata ccgaagaatc ctgtttccat ataaacagac acttcttcag gtaataaggc
     8101 accagctcta gcacgtaaat tattattttc ccaaaacgga gcaatacttc taagttgttc
     8161 ttttgtttct tctgtaatat agaaaacatc tccatcacgc ttttcaaaaa gctccaactc
     8221 attgagaaca aattctagcg tatattccgg aaaaatagga gcatctttat tggaagaagc
     8281 ttgatttccc gcaatcatag attcttcttc aatatagata gacatatttt ccaaaatttc
     8341 tttcagcata tatgcacgtt ttaggacatt aggttgttcc ttgtatcgtt cataggcgcg
     8401 tgttgctaga actgctctct cagcatcaat ataaggtttt ttatttaaga catcttctcg
     8461 atatttgttc atcctttcag taaggctgcc aaaatattct gtcttgattg ttgttcttgc
     8521 tacttctgta tttaccatcg taagcacctt tctttcgaaa atatttttat ttctttcgaa
     8581 aatataatac cgatttttat aaaaattttc aagcttttaa ctagaaaaat tttaactctc
     8641 taatttttag cacaaatgta tgagtttaac agtaacacac aaatacctct aagattattt
     8701 gatttcattt tggtaagcta aacgatcctg tatcttaaag aatggaaagt aaaccaatgt
     8761 agacatcgct aatatcacca gctgagtaat aactccttgc catccaccca ccaagaatcc
     8821 tgatagaata gctggtgtac tccaaggcaa tgttacccct gagaatggct gcatgaaacc
     8881 tgttgcaata gatccatata ctatcgcagc tgcaagtaca ggaacaagaa tgaaaggtac
     8941 aaacataact ggattcatga caatcggaaa tccaaataca actggctcat ttacgttaaa
     9001 tattgctgga aaagctgcaa cttttcctaa ggcttggtat tgttttgatt ttgctgcaaa
     9061 aagcatggca actacaagac caaacgtaat ccctgaacct gatagaatta aaaatgaatc
     9121 taaaaactgt tgagtaacaa tatgtgctcc attttctaat gatagattag cagaggctaa
     9181 catagcttta ttagcatcaa gattagataa aagaagagct gtcactactc catttactac
     9241 cgattgccca tgaacaccaa accaccacaa aaatgatata aagaatgcaa ttccaatagc
     9301 accatacaaa gatccagtta aaccttgcaa cggaacttga atagcagaat aaatcatttc
     9361 tatgaatgtc ccgccattag tcaatgagct cgctaaaata tatacaatca tagaagataa
     9421 gaaaattaca aatgctggaa tcattgtttc aaactgtttg gcaatagctt gtggaacttg
     9481 ttctggcatc ttaataacaa tttttctctt tataaagaag gtataaatac ttcctactac
     9541 caaacctata atgatagcac cgataattcc ctggcctcca aaccaaactt tactaatagc
     9601 gtccccaatc gcctcacctt gtttagggat ataagatgat cttagcaaaa taaagaatgc
     9661 agatacagat agaactccag ctggtaaagc ctctactccg ctattcttag cataagaata
     9721 ggcaattgaa aaacaagaaa ttagacccat aatagcaaaa gttcctgaat atacttgcat
     9781 aaacggctct gtccaattag ctccaaaaac actagcaatg ctcttattta atccttcgaa
     9841 cggcaattgt cccataatca agaataaact accaactact gtcaatggca aaattgctaa
     9901 catcccatct tttagagcta taatgccacg catattcaca aacttcatca tcggtgcaat
     9961 gattttctga acatccatct ttgccataat aaatctcctt ttcttaccca ctaatcaaag
    10021 atagggccaa atctaatact tttttcccat ctaacatacc atagtccatc atcggaataa
    10081 cagctatcgg aacatcacac ttatcacaaa tttcttttga tttatctaat gtataagcaa
    10141 cttgtggacc caatagtgca acatctatat ttggcgcata atccgctaat ttagactgag
    10201 aaaacgcctc tatttctgcc tcaactccac tagattgcgc tgcaattttc atattattta
    10261 caagcatacc agtagaaaaa cctgctgcac aaaacaaacc aatcttcacc attatgtttt
    10321 cctcctctat gttaataaca atgataatac tctagtatta ttttttatga agttcttttc
    10381 tcaaactaat aatttctttg attaaattaa tctccgtcat actagtcatg agatgatctt
    10441 gtgaatgaac aaagagagct gtaatttctg tctttgtacc agacgcttct tgtgccaaga
    10501 attttgtttg tagattatgg gcttctaata aggcggcatc agctaactgg atttcttctt
    10561 cacaccgttc acatgtacca ttttttatgt aatctaacgc tttataaata tgttgtttcg
    10621 catcaccagc atataaaatt aaacccatga taatttgatc tggaacaata atttccataa
    10681 attcctcctc atttcactat gtaaaaagct tttttcattt gaaattttaa aattttcttt
    10741 tgtaactata gaatacaact acataataaa gttgtcagtc caaaaacaac agataaacta
    10801 tttttgttta tataagcaca aatgtgcaat caactaaaaa gaaaaacgat aaact
//
LOCUS       DAVKDZ010000069        10372 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000069 DAVKDZ010000000
VERSION     DAVKDZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.070"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(330..551)
                     /locus_tag="VWZ30_001695"
     CDS             complement(330..551)
                     /locus_tag="VWZ30_001695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335386.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGIEQGREEGLEEGLKVGL
                     VNLVRQGLLTPEVASEQLGMTVAEFESLL"
     gene            complement(544..660)
                     /locus_tag="VWZ30_001696"
     CDS             complement(544..660)
                     /locus_tag="VWZ30_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9335387.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            complement(875..1228)
                     /locus_tag="VWZ30_001697"
     CDS             complement(875..1228)
                     /locus_tag="VWZ30_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9335388.1"
                     /translation="MTVRHPGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAKNVTIL
                     EGSNIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINRLWAYLCSQV
                     NQNLEKIRQREGDTH"
     gene            complement(1346..2314)
                     /locus_tag="VWZ30_001698"
     CDS             complement(1346..2314)
                     /locus_tag="VWZ30_001698"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9335389.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(2459..3274)
                     /locus_tag="VWZ30_001699"
     CDS             complement(2459..3274)
                     /locus_tag="VWZ30_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEU9335390.1"
                     /translation="MKKNSILFIFILLLCIGLQYETIYYTDGSISGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKNALILSLLGGMFLSGWLSSFANTYIHDLLGGLFPD
                     SAFLNAFESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGTASGLGFQMIEDIG
                     YIRTDLPEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKIGKKQGLI
                     FLGLALGTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(3299..3796)
                     /locus_tag="VWZ30_001700"
     CDS             complement(3299..3796)
                     /locus_tag="VWZ30_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU9335391.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENGPF
                     QEYLKEELGVDMSDQDFLPFQDIEESVREDMKLLIESSLIPDDVIISGAIYDVDTGSM
                     TVVEL"
     gene            complement(3869..5233)
                     /gene="radA"
                     /locus_tag="VWZ30_001701"
     CDS             complement(3869..5233)
                     /gene="radA"
                     /locus_tag="VWZ30_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU9335392.1"
                     /translation="MIAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEIEVAEVK
                     NARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGK
                     STLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEV
                     ERIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKE
                     GTLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLN
                     PSQVFLEERLDGATGSSIVVTMEGMRSILAEVQALVTPTMFGNAKRTTTGLDFNRASL
                     IMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVG
                     ELGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITPPKEIQVIGVTTIQEVLK
                     KVFA"
     gene            complement(5244..5759)
                     /locus_tag="VWZ30_001702"
     CDS             complement(5244..5759)
                     /locus_tag="VWZ30_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9335393.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSDMGRQQVQKLSK
                     NPLLSSAEIIVSSAVARALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENRGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(5761..6204)
                     /locus_tag="VWZ30_001703"
     CDS             complement(5761..6204)
                     /locus_tag="VWZ30_001703"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU9335394.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(6261..6358)
                     /gene="ffs"
                     /locus_tag="VWZ30_001704"
     ncRNA           complement(6261..6358)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VWZ30_001704"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(6391..6858)
                     /locus_tag="VWZ30_001705"
     CDS             complement(6391..6858)
                     /locus_tag="VWZ30_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU9335395.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCILFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVETGILEDECAAIMQDFFRNRRKK"
     gene            complement(7059..8345)
                     /locus_tag="VWZ30_001706"
     CDS             complement(7059..8345)
                     /locus_tag="VWZ30_001706"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU9335396.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKEIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(8576..8812)
                     /locus_tag="VWZ30_001707"
     CDS             complement(8576..8812)
                     /locus_tag="VWZ30_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEU9335397.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            9078..9889
                     /locus_tag="VWZ30_001708"
                     /pseudo
     CDS             9078..9889
                     /locus_tag="VWZ30_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000530076.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9924..10133)
                     /locus_tag="VWZ30_001709"
                     /pseudo
     CDS             complement(9924..10133)
                     /locus_tag="VWZ30_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10299..10372)
                     /locus_tag="VWZ30_001710"
     tRNA            complement(10299..10372)
                     /locus_tag="VWZ30_001710"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10336..10338),aa:Asn,seq:gtt)
BASE COUNT         3288 a         2455 c         1690 g         2939 t
ORIGIN      
        1 atatttctat aaattaattt gactttcctg atagagttat tcacatctta tttcaactca
       61 ctatagaagg aggaaaagga gggctgggtg gctaacttca ttatagaact ttcaaaaaga
      121 tgaaaaaata gcgtcaaaga ggcttttttc tctttaaaac aggaaatctc aaagacaatc
      181 ttgcgactat ccaaactgta cgaataacga gacataactt acttcgctaa tactatctta
      241 taaaaagata gaaatgtcaa gcaaaaatga caagtatttt gataatagac ttgccatccc
      301 atcaactttc tactatataa agtatgaact catagcaagg actcaaactc agccacggtc
      361 atgcctaatt gctcactcgc aacctcaggt gttagaagac cttgacgtac tagatttact
      421 aaacctactt ttaatccctc ttcaaggcct tcttctcgtc cttgttcaat accttcagcc
      481 ctagcggttt ccaaggcata gtcctgtgct aacaagactt gttcttctcg catatgtagt
      541 tgactaaaca ttttcctgtc ctcctcggac cagctcttgt agtccagcag ttgatttgct
      601 tgggtaatgg ctcgttgcgg ttgctgggta aagggcttgt tgccgaaaaa ctccaaccat
      661 ggcttgcgaa ccttgtcttt gctggtttct ctgtattttt taattccaag aatgccatct
      721 taaccagatg gttttcctgt ccattgttgg taatcgccaa tacctcacct gttgtgtctt
      781 cgcgcatact aaagctatga aaagccaggt catctgagaa ataattacta tccacgatag
      841 caatggcgta aacaggagcg atgtgtttgt agctctagtg agtatcacct tctcgctgac
      901 gaattttttc aagattttga ttaacctgac tgcacaggta agcccacaag cgattgatga
      961 aaaaattctg atgatggact tgaatctcaa taattacttg agtaccgtta tccaactccg
     1021 ccaagacgtc tatactggta taaaaatcct gcaccgagta aggcatggag agtaatacgt
     1081 gaatattgct accctccaaa atggtcacat ttttggctgg caaatccagc atatcgcgaa
     1141 taaattgaca agtgatttct ggattgctaa agattttctt agctaccaag tcattggttg
     1201 ggctaatgcc cggatgacgt acagtcatat ttcttctctt tttattatat cacagtttta
     1261 aatctagctt tactagattc accgctacta tctatttatt tggaaaaaag acgaaaaaac
     1321 ctgagaatca tctcaggctt ggtcattaaa tttttttctc aatatcgaaa agtggagaaa
     1381 gtggtcgttt ttcatgaata cgtacgatag catcccctag gagatgagct attgaaatct
     1441 gctcaatctt atcaatcaaa cgctcttctg gcagatagat ggtatccaaa acaaccaatt
     1501 tcttaatagc tgatttttgg atattgtccg tagcaggacc agaaagaact gggtgcgtac
     1561 agcttgcata gacttcaaca gcaccagctt ccgcaagagc atctgccgca tgacaaatcg
     1621 ttccagcggt atcaatcata tcatcaatca agatacaagt cttgccttca accttaccga
     1681 tgatgttcat aacttcacta gtattcatct tatcaacgct acgacgttta tcaataatag
     1741 cgatagatgt tttcaaaaat tctgccaact tacgagcacg agtcacccct ccatggtccg
     1801 ggctgacaac cacatagtca gaaccaacca taccacgacg ctcaaaataa tctgcaatca
     1861 gaggagcacc catcaaatga tccacaggaa tatcaaagaa tccttgaatt tgcgcagcat
     1921 gcaagtcgat ggtcaataaa cgatccactc cagctacttc aagcatattt gcgacaagtt
     1981 ttgaagtgat tggctcacgc gctctcgcct ttctatcctg acgtgcatac ccatagtaag
     2041 gcatgacaac attgacagat tctgcactcg cacgcttcaa agcatctacc ataatcaaaa
     2101 tttcaagcag attgtcattt acaggcgaac tagttgattg taagataaag acgtgtttcc
     2161 cacggattga ttcttcaatg ttgacctgaa tctctccatc tgaaaattgg cgaacacttg
     2221 atttccccaa ctctatccca atctcctgcg ccacacgttc tgccaattct ttattagaag
     2281 aaagggcaaa cagctttaaa tcagaaaaag acatgatttc ctccggtata tatgtataac
     2341 ttgtgctttt cacaagattt tccatctacc attgtagcgc tttttgcact atttttcaat
     2401 caaaaataaa agaagggcac catatttgta cccttgcatc attcttttga aaaatattct
     2461 aggtcatcaa ctcattatgt ttctcaacaa agcaataagc atgataaaaa ccatagagag
     2521 caatagccgt aaccactgga atcgctaaag gcaactctgt ttccaactcc acaaaaggag
     2581 agttaaacaa gaagtgagtt cccaaggcta gacctaggaa aataagtccc tgtttcttgc
     2641 caatcttctg tcctttatag gctctgtaaa gcaagtaaac ccccaccaca gccagacctg
     2701 aaaaagtcca gtgagaggca attcctgaga tgatacgctc taaaatcctc gaaatagtaa
     2761 agtcaaatcc ctccggtaaa tccgtacgaa tatagccaat atcctcaatc atttggaatc
     2821 ccaaaccaga agcagtccct agtaaaaaca aagattttaa ttttcgcaca ggaatcaaag
     2881 ctaaaacaaa aacaagtggc aacaatttca agggctcttc taccaaagga gccacaatcg
     2941 cactttcaaa ggcatttaaa aaagcactat ctgggaaaag accacccagt aaatcatgga
     3001 tataggtatt cgcaaagcta gacaaccagc ctgaaaggaa catcccaccc aataaagaca
     3061 agatcaaggc gttctttggc aattcccatt ttttcccaat acggaaaagg aaataaagag
     3121 ccggaatcat gtaaaagaga gctagaaaga tagaaactcc cattagtcca tattccgcgc
     3181 ctgatatcga accatccgta tagtagatgg tttcatactg taaaccaata catagcaata
     3241 aaataaaaat aaataaaata ctgtttttct tcatacactt tctttctaaa tgaatttttt
     3301 acaattctac aactgtcata cttcctgtat ccacatcgta aatggcacca gagataatga
     3361 catcgtctgg tattagggaa gattcgataa gcagtttcat atcctctcgc acactctcct
     3421 ctatatcttg gaagggcaag aagtcctggt ctgacatatc gacacctagt tcctctttca
     3481 gatactcctg aaaaggacca ttttcaaagg tctgagcacc acagtctgta tggtgcagca
     3541 ccacaatctc tcttgtcccc atttgttgtt gggaaataac tagcgaacga atcatgtcct
     3601 cagtcactcg accacctgca ttccgcaaga tatgagcatc cccaagtgcc aagcctagag
     3661 cttgtgcaac gtgcagacgt gagtccatac aggtcacaat ggctactctt gttttgggtt
     3721 taagtggcag atttaactgc ccatgtaggg caacataagc ctgattggct tgcataaact
     3781 gttcaaaata cgacacgatt ccctccttga aaatttgata gtcaaatatt tctcctatct
     3841 tatcattttt aagaggattt gtcacggatt atgcaaaaac ctttttcaaa acttcctgaa
     3901 tggtagtcac accaatcacc tgaatttcct tgggcggagt gatccctgtc aaagaatttt
     3961 tgggtacata aatcttagta aagcccagtt tagcagcttc gttgatgcgt tgctcgatac
     4021 gattcacgcg ccgaatctct cccgtcaagc ccagttcgcc tacaaaacat tcctgaggat
     4081 tggtaggctt gtctttatag ctcgaagcaa tagcaaccgc aacggccaag tctatcgcag
     4141 gttcatccaa tttgacaccg ccagcagatt tgagataggc atcctgattt tgcaagagaa
     4201 gacctgcccg cttttccaaa acagccataa tcagactagc acgattaaaa tcaagtcctg
     4261 tcgtcgtacg cttagcattt ccaaacatgg tcggtgtcac caaagcctga acttccgcca
     4321 aaatcgaacg catcccttcc atggtcacaa cgattgatga accagtcgct ccatccaaac
     4381 gctcttctag gaaaacttga ctcggattga gtacctcaac caaaccgccc gactgcatct
     4441 caaaaatccc aatttcattg gtagaaccaa aacggttttt gaccgctctc aagatacgaa
     4501 aggtatggtg gcgctcccct tcaaagtaaa gcaccgtatc caccatatgc tccaacatac
     4561 gaggtccagc caaggtaccc tccttggtca catgacctac gataaagatg gcgatattat
     4621 ttgtcttagc caactgcatg agctcagccg tcacctcacg cacctgagaa acagacccct
     4681 gcacccctga aatctcagga gacataatgg tctgaatgga gtcaataata aggaaatctg
     4741 gttggatgcg ctctacttct gcacgaacac tctgcatatt ggtctctgca tagaggtaaa
     4801 actcgctatc aatatcaccc aagcgctctg cacggagttt aatctgctgg gcagactctt
     4861 caccactgac ataaagaacc gttccaactt gagacagctg ggtagagact tgtaggagaa
     4921 gggttgattt tccaatccca ggatccccac cgatgaggac gagacttcct ggtaccactc
     4981 cgcctccaag tacacggttg aattcctcca tctccgtctt ggttcgattg acattgattg
     5041 aagtcacctc agccagtttc atgggcttgg ttttctcacc tgtcaaggac acacgcgcat
     5101 tcttgacctc ggcaacctca atctcttcta caaaagaaga ccaagaccca cagttggggc
     5161 atcgtcccag atatttaggg gaattatacc cacaattttg acatacaaat gtcgcttttt
     5221 tctttgcgat gataaacctc tttctatatc tctaactcac actcaatcac ttggcaaaaa
     5281 tcaatcttct catttggcac aaactggcgc atgagcattc gatgagcaac aactaccaca
     5341 gtctgatgtt ctcgatactt agacatacat tctagaaacc gagacttcat ttccgtagct
     5401 gtctcatatt gaataggact attaggaagt aactcccccc tgttttctaa aaacagtctt
     5461 ctagctgttt caaagttttc tattcctgtt ttatagacct gccattcatg taataaaggc
     5521 tctactctta aaggaagacc cgtagcacag accacatacg aagccgtttc taaagctctt
     5581 gcgactgcag aagatacgat tatttcagct gacgagagta aaggattttt gctcaatttc
     5641 tggacttgct gccgtcccat atcagacaag ggtgccaaat ctatcccaaa tcctatataa
     5701 gaacgctcct ctaactcacg gtaatctggc tccccatgac gtacaaagat aatcttcatt
     5761 ctagtgccct gtcgatccaa atccaccagt tcgaacgcca tcagccgcat ctccatctgc
     5821 aattaagaaa gtagcaaaaa cagcctggac aatacgctcc ccaacttcaa gaacaacctc
     5881 ttggtctgtg atattcttca tctgcgcaaa aatatgccct tcatttccag gatttccata
     5941 ataatcccca tcaatgaccc caactgagtt aattaaaacc aagcccttct tacgaggatt
     6001 tgaagaacga tcatagaggt agagaacctc agtcggctgc atataagcct taacccctgt
     6061 cggaaccaag acaatctctc ctggcgcaac aactgtacgc acagcaacct ttaagtcgta
     6121 accagccgca tgcgctgtct cacgcttggg caataaattt tcatctgtaa aactcgaaac
     6181 caattcaaaa ccacgaattt tcataatttt ctcttttcta ttatcattta ttctagatta
     6241 ttctatctta tttattcgga aaaagcacga aaaaaagagc acacaattca aatcgcttag
     6301 ggctgctgga ttcctcccct gacccgcttc acgcagaact gttgctccac tatttattat
     6361 atcacattcc tattcatttt aaaagcaaaa ttattttttc cgtctatttc taaaaaagtc
     6421 ctgcataata gctgcgcatt catcttccaa aattcccgtt tcaacctcta cacgatggtt
     6481 aagacgctca tctgtcaaga tatcgtacaa acttccagca gcgccaaatt tctggttttt
     6541 agccccatag accacatttg gaatacgggc aagcccaatc gccccactac acatgacaca
     6601 aggttcaatg gtcacaaaaa gtatgcaatc cagcaagcgc cagctctcct cactcaagtt
     6661 cgcatcctct atagccataa tttccgcatg cataaccgct cgctgtaatt cctcacgcgc
     6721 attatgccca cgaccaatga tttccccatc tttgacaatc acacaaccaa ttggaatttc
     6781 atcgtgttca agagcaatct cagcctctct caaagcctcc ctcataaaga cttctttttc
     6841 ttcaagcgta taatacatct cttttctctt ttcctactta tcgattttat tattatatca
     6901 tgaatcccaa cacaaaaaaa gccaccgaat gcggtgactc tatagggaga ttattatgaa
     6961 aaagaaaagt ttaggatatt tgttacaaca agttaggagg tcttcttgta actgtctata
     7021 gtatacccga cctatcttaa acaaatctta aaaatctctt aggaccaaac actttctaaa
     7081 atatttgttt gttcacgacc aggacctact gagaaagtag aaatacgaac gccaaccaat
     7141 tcactcacac gacgaacata gttacgcgca ttctcaggaa gatcttccaa attgcgaact
     7201 ccggtaatat cttctgacca acctggcaac tcttcataga taggcttgca acgtttcaat
     7261 tgctcaagac tagctggata gtagtcaata cgttgaccgt caagatcata ggccacacag
     7321 attttcacag tatccaaacc gctcaaaaca tcaatagagt tcaaagaaag gttagtaata
     7381 ccagaaacac gacggctatg acgcatcaca actgagtcaa accaacctac acgacgtgga
     7441 cgaccagttg ttgtaccata ctcatgaccc acttcacgga tacgttctcc cacttcatca
     7501 aacaactcag ttgggaaagg accatctcct acacgactcg tataagcttt acatacacct
     7561 acaaccttgt caatcttgct tggaccgaca ccagaaccaa ttgtcacacc accagctaca
     7621 gggtttgatg acgtaacaaa tggataagta ccttggtcga tatctagcat aacaccttgt
     7681 gcaccttcaa aaagcacacg tttgccatta tcaagcgcat cattcaagat aacagatgta
     7741 tctatcacgt atttcttgat ttgttgacca tattcgtaat attcttcaaa aatatcatcg
     7801 aaaacaatct ctttactgtc atacaatttt tcaaaaagac gattcttttc agcaaggtta
     7861 cgttctaaac gctcacggaa aatatcttta tctaaaagat ctgcaatacg aattccaaca
     7921 cgagcagcct tgtccatata agctggacca attcccttaa ttgtcgtacc aatcttattg
     7981 tcgcccttag cttcttcttg caagcgatcc aactcgatat gataaggcaa aataacatgc
     8041 gcacgatcag aaatacgcaa gttatcagtt gttacacctt cctcatgaag atagctcaac
     8101 tcttttacaa gagatttagg atttacaacc ataccattcc caatgacaga tattttttca
     8161 gggaagaaaa tcccagatgg aatcaagtgc aacttaaatt tcttaccgtc aatcacaatc
     8221 gtgtgaccag cattatcacc accttggtaa cgtgcaatca cttctgcatt cgctgaaagg
     8281 aagtctgtaa tcttcccttt accttcatca ccccattggg tacctacaac aacaactgaa
     8341 gtcataatct tgtctgagcc ctcaggctct tcctttctca catacatggc aggactctca
     8401 cctgcaatta tatcttacaa tttattataa taaaaaatcg cctttttatc aagaagaaac
     8461 aatagaaaga tttgctattt ccaactatta aaaaatgatt tagaaaaatt actagctatt
     8521 tactattatc tttccataaa agagtaaatt agttcggaaa tttagtaaaa ttacctcaac
     8581 aagaaataaa accccgattc attaccaatt tttcaagata caaacgataa gcaacacgat
     8641 aatggtaaac gataaaatcc ctacgacaac caatgccata tctcactaaa taataaatta
     8701 aaaatttaaa atgaacatgt tcccagtcaa aattatcacc aaatgtagga ccatactctt
     8761 cttcaatact atcatagaaa ttagccatct gctcataaat tttttgtaac ataatcaaat
     8821 actccttttc ttttttataa acttattcta acaaaaaatt ttacacattc actatcaatt
     8881 cctgaattgt taaaatacca gcctcaacaa gataaaaata ggaaaagttg acagaataga
     8941 aacaaatttg ctacctaaat ttcaaacgat taaagttcta aaaagaccgc cttaaaactt
     9001 ttgggtaaat cctattagaa ctataatatt tttatatagg tattatggtt ctgattttct
     9061 agtataaaaa aattcaaact agaatttttc ttagaaaagg ccgttttaag tgctatcatt
     9121 gctcaaaaat ggaggtcgct gagacttcta tcgtcaagaa gaatcaccaa atccctcgta
     9181 tcatcaacca aaagattgct caaaagttaa ttgaaaagat ttctatgacc gatattgctc
     9241 atcagctatc agctggccat tttaacttca actgtcattc gaaagctcaa tgacttccac
     9301 tttgagcatg atttttcgcg tcttcctgag attatgtcct aggacgttga aacagtccgg
     9361 ggagtgactg tttcaatcgg gagatggaga tgagctttat tgctcaggac tttgataagc
     9421 tcaatatcat aactgttctt gagagcagaa cacaagccat catccgaaat cccatggata
     9481 caaggctatc aagcgctact ggaagctcat tcaacaagat agtcagaaac tgagtgataa
     9541 acgattttat cgccctactt ttcgtatgca cttgactaat aaagaaattc tagataaact
     9601 tttgagctat tccgaaaact tgaaacacca ctataatctc tatcagctct tgcttttcca
     9661 cctctttttc agactctgtt taaaaccttc ctcaaagata aagagaaaat cgtcaacgcc
     9721 cttcaattac cttattccaa attccaacgc caaactggaa gccaccaata atctcatcaa
     9781 acttatcaag cacaatgcct ttggttttag gaactttgaa aacttcaaaa aagaaaggac
     9841 gaaatttgtc ctttctatat cttcgctttc ttcaacccac tacagttgac aaagaaccga
     9901 aaaatgtctc gcctctaata tagtcaattg aaacaagaac aagacaaaag agcctcgtaa
     9961 aaggtattgc aacgtggtaa tacctttttg aggttctttt tgatatgagc ccatgttttc
    10021 tcaataggat tgtactcagg tgagtaggga agaagaggta aaagtttatg cccaaactct
    10081 tcacataaaa gttctagctt acccattcta tggaatcttg cattgtccat aatatagtag
    10141 attgaaacta gaatagtaca cctctacttc taaaacattg ttagaattcg attttactgt
    10201 cctgatcgat ttgtcctatt cttatttcat tttactataa cacgaaatat attattatta
    10261 cataacaaaa aagaggtcta aacctcttaa ctaaattact ccgccagtag gactcgaacc
    10321 tacgacatca tgattaacag tcatgcgcta ctaccaactg agctatggcg ga
//
LOCUS       DAVKDZ010000070        10342 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000070 DAVKDZ010000000
VERSION     DAVKDZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10342
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.071"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            331..1377
                     /gene="pheS"
                     /locus_tag="VWZ30_001711"
     CDS             331..1377
                     /gene="pheS"
                     /locus_tag="VWZ30_001711"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU9335398.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1377..1886
                     /locus_tag="VWZ30_001712"
     CDS             1377..1886
                     /locus_tag="VWZ30_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9335399.1"
                     /translation="MIRKVEMADVEVLAKIAKQTFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1963..4368
                     /locus_tag="VWZ30_001713"
     CDS             1963..4368
                     /locus_tag="VWZ30_001713"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU9335400.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNIGEEEARQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPENAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAATDALSVGIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASMISELAGA
                     TVRKGIVSAGELDTSDVEVSSTLVDVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDCLPTSLPKDDGTAGELTATQKLRRQVRTIAE
                     GAGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLEVTYTATAEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(4436..5203)
                     /locus_tag="VWZ30_001714"
     CDS             complement(4436..5203)
                     /locus_tag="VWZ30_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9335401.1"
                     /translation="MLLLSLILLLFGTDKTLSSASNKTKTLKLVTWNVANQIEAQHIE
                     RIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGLSMANYDIFTSPPTNNGIAPV
                     TVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDIIALHTAPPLPGLMEIWKQDLN
                     IIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDALNALPPFERGTWNSQSPKLFN
                     ATIDHILLPKNHYYVRDLDIVSFQNSDHRCIFTEITF"
     gene            complement(5229..5438)
                     /locus_tag="VWZ30_001715"
     CDS             complement(5229..5438)
                     /locus_tag="VWZ30_001715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335402.1"
                     /translation="MIKTFLSALSVILFVIPIIAYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFLFFFPIFLQEK"
     gene            5593..6081
                     /locus_tag="VWZ30_001716"
                     /pseudo
     CDS             5593..6081
                     /locus_tag="VWZ30_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6165..6431
                     /locus_tag="VWZ30_001717"
     CDS             6165..6431
                     /locus_tag="VWZ30_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9335403.1"
                     /translation="MEGCPSEFFSKEEILESDMRVAIMSELIEARHEQGISQKKLEEF
                     SGVSQPVIARMETGKTSPQLDTVLKVLSSLGKTLVVVSLEQRKS"
     gene            6799..9048
                     /gene="metE"
                     /locus_tag="VWZ30_001718"
     CDS             6799..9048
                     /gene="metE"
                     /locus_tag="VWZ30_001718"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU9335404.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEILEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLNLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVATYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            9112..9978
                     /gene="metF"
                     /locus_tag="VWZ30_001719"
     CDS             9112..9978
                     /gene="metF"
                     /locus_tag="VWZ30_001719"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU9335405.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNDNIISALQDMQELAPYFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
BASE COUNT         3173 a         2021 c         2259 g         2889 t
ORIGIN      
        1 tcatcttatt tcaatccgct ataaagttag ataagaataa gctaacaagc agaagtaaga
       61 taggtcttgc aagaatggta aaaatctgat aaactaagta gtaattgaat aggaatctga
      121 aagactagta gcccaaggga agtatatcag ggaggagagc cgtgactgca agctctctat
      181 atgaaagctg ggtgaattca cttgcgcatg gatttagaaa tgaaggtctg acttgtcaga
      241 tgaaaacgga tggtaccgcg tgtcaacgct ccgagtggag tttttggcat gtggttttct
      301 ttttatctac gagagactga tggaggaaat atgtcaacta ttgaagaaca attaaaagcg
      361 cttcgcgaag aaacgctggc tagcttgaag cagattactg ctggaaatga aaaagagatg
      421 caagatttgc gtgtctctgt ccttggtaaa aagggttcgc tcactgaaat cctcaaaggg
      481 atgaaagatg tttctgctga gatgcgtcca atcatcggga aacacgtcaa tgaagctcgt
      541 gatgtcttga cagctgcttt tgaagaaaca gctaagctct tggaagaaaa gaaagtcgcg
      601 gctcaactgg ctagcgagag tatcgatgtg acgcttccag gtcgtccagt tgcgactggt
      661 caccgtcacg ttttgacaca aaccagtgaa gaaatcgaag atatcttcat cggtatgggt
      721 tatcaagtcg tggatggttt tgaagtggag caagactact ataactttga acgtatgaac
      781 cttccaaaag accacccagc tcgtgatatg caggatactt tctatatcac tgaagaaatc
      841 ttgctccgta cccacacgtc tccagttcag gcgcgtgcta tggatgccca tgatttttct
      901 aaaggtcctt tgaagatgat ctcgccaggg cgtgtcttcc gtcgcgatac ggacgatgcg
      961 acccacagtc accaattcca ccaaatcgaa ggcttggtag ttgggaaaaa tatctctatg
     1021 gctgatcttc aaggaacgct tcagttgatt gtccaaaaaa tgtttggtga agagcgtcaa
     1081 attcgtttgc gtccatctta ctttccattc acagagccat ctgttgaggt ggatgtttct
     1141 tgcttcaaat gtggtggaga aggctgtaac gtatgtaaga aaacaggttg gatcgaaatt
     1201 atgggggccg gtatggttca cccacgtgtc cttgaaatga gtggtatcga tgcgactgta
     1261 tactctggct ttgcctttgg tcttggacaa gagcgtgtag ctatgctccg ttatggaatc
     1321 aacgatatcc gtggattcta ccaaggagat gtccgcttct cagaacagtt taaataatga
     1381 ttagaaaagt agaaatggca gatgttgagg tgttggctaa aattgccaaa caaacctttc
     1441 gtgaaacctt tgcgtatgat aatacggaag agcagttaca ggaatacttt gaagaggctt
     1501 atagtctgaa aactttgtca actgagttgg gaaatcctga ctctgaaacc tatttcatta
     1561 tgcatgagga ggagatagct ggttttctca aagtcaactg gggaagtgct caaactgaga
     1621 gagaattaga ggacgctttt gaaattcaac gcctctatgt gctacaaaaa ttccaaggat
     1681 ttggactagg taagcaactg tttgaatttg ctcttgaact tgctacaaaa aatagttttt
     1741 cttgggcttg gttaggtgtt tgggagcata atacaaaagc tcaagcgttt tataaccgat
     1801 atggttttga aaaatttagc caacatcatt ttatggttgg tcaaaaagta gatacggatt
     1861 ggttactgag aaagaaatta aggtaagaag atgattcttc tattgaaatg ataggaaagg
     1921 aaaagaacta gagtgacaac tgtcattctg gagaactaaa ttatgcttgt atcttataaa
     1981 tggttaaaag aattggtgga cattgatgtg ccatcacagg agttggctga aaaaatgtca
     2041 accacaggga tcgaggtcga aggtgtcgaa tcaccagctg ccggtctttc aaaaattgtc
     2101 gtcggtgagg tcttgtcttg cgaagatgtg ccagaaactc acctccatgt ttgtcaggtt
     2161 aacattggcg aagaagaagc tcgtcaaatc gtttgtggtg ccccaaatgt gcgtgcaggc
     2221 atcaaggtta tggtggctct tccgggagct cgtatcgctg acaattacaa aatcaaaaaa
     2281 ggaaaaatcc gtggcttaga gtcacttgga atgatctgtt cacttggtga attgggtatt
     2341 tctgactcag ttgtacctaa ggaattcgca gatggtattc aaattttgcc agaaaatgcc
     2401 gttccaggtg aggaagtctt ctcatatcta gacttggatg atgaaatcat cgaactttct
     2461 atcacaccaa accgtgcaga tgccctttct atgtgtgggg ttgcgcacga agtagcagcc
     2521 atctatgaca aggcagtcaa ctttaaagaa tttactttga cagaaacaaa tgaagctgcg
     2581 acagatgccc tttctgtggg cattgagaca gacaaggcac cttactatgc ggcccgtatc
     2641 ttggacaatg tgaccatcgc accaagtcca caatggttgc aaaaccttct catgaacgaa
     2701 ggcatccgtc ccatcaataa cgttgtagac gtgacaaact acatccttct ctactttggt
     2761 caacctatgc atgcctttga cttggatacc tttgaaggga ctgacatccg tgtgcgtgaa
     2821 gcgcgtgctg gtgaaaaatt ggtgaccttg gacggtgaag aacgtgactt ggacgtgaat
     2881 gacctagtca tcactgtcgc agacaagcca gtagcccttg caggtgtcat gggtggtcaa
     2941 gcaacagaaa tctctgaaaa atctagtcgt gttgtccttg aagctgctgt tttcaatggc
     3001 aaatctatcc gtaagacaag tggtcgcctg aaccttcgtt ctgagtcatc ttctcgcttt
     3061 gaaaaaggaa ttaatgtggc aacagttaat gaagcccttg atgcggcagc tagcatgatt
     3121 tcagagcttg caggcgcgac ggtgcgtaag ggtatcgttt cagcgggtga gcttgatact
     3181 tcagatgtag aagtttcttc aacccttgtt gatgttaacc gtgtcctcgg aactgagctg
     3241 tcttatgctg atgtagaaga cgtcttccgt cgtcttggct ttggtctttc tggaaatgca
     3301 gacagcttta cagtcagcgt accacgtcgt cgttgggata tcacgatcga agctgacctc
     3361 tttgaagaaa tcgctcgtat ctatggttat gactgcttgc caactagtct accaaaagac
     3421 gatggtacag caggtgaatt gacagccaca caaaaactcc gccgtcaagt tcgtactatt
     3481 gctgaagggg caggcttgac agaaatcatc acctatactc taacaactcc tgaaaaagca
     3541 gttgagttta cggctcaacc aagtaacctt acggaactca tgtggccaat gacagtggac
     3601 cgttctgtcc tccgtcaaaa tatgatttct ggtatcctag ataccgttgc ttacaacgtg
     3661 gctcgtaaga acaaaaactt ggccctttac gagattggaa aagtatttga acaaacaggt
     3721 aatccaaaag aagaacttcc aaatgagatc aacagctttg cctttgcctt gacaggcttg
     3781 gttgctgaaa aagatttcca aacagcagca gttccagttg atttcttcta tgctaaagga
     3841 atcctagaag cgctctttac tcgcttggga ctagaagtga cctatacagc aacagctgag
     3901 attgccagcc ttcacccagg acgtacagca gtcatttcac tcggtgacca agttcttggt
     3961 tttcttggcc aagtgcatcc agtcactgct aaggcctacg atattccaga aacgtatgtg
     4021 gctgagctta acctttcagc tatcgaagct gcgcttcagc cagcgactcc atttgtagaa
     4081 atcaccaaat tcccggcagt cagccgtgac gttgcccttc tcctcaaggc agaagtaact
     4141 catcaagaag ttgtagatgc tatccaagct gccggcgtga aacgtttgac agatatcaaa
     4201 ctctttgacg tcttctcagg tgagaaattg ggacttggta tgaagtccat ggcttatagc
     4261 ttgaccttcc aaaatccaga agatagctta acggacgaag aagtcgcacg ctatatggaa
     4321 aaaatccaag catcgctcga agaaaaagtc aatgcagaag tgcgttaaac gatagtatga
     4381 tacaaattcc ccctctagtt tatgaacact agaggggtga ttttatataa aataattaaa
     4441 atgtgatttc tgtaaaaata catctatgat cagagttttg aaaacttaca atgtctaaat
     4501 ctctaacata gtagtggttt ttaggcaata agatatgatc tatcgttgca ttaaaaagtt
     4561 ttggactttg gctattccaa gttcctcttt caaaaggtgg aagtgcattc aatgcgtccc
     4621 tatgagagct tatttttgca agtgctccat gacgcatagt tgcattaaaa tcacctgcaa
     4681 taatagcctt tggatatttt gaagccaatt gattatgaat gatgtttaag tcttgcttcc
     4741 agatttccat taaacctggc agaggaggag cagtatgcaa ggcaatgata tctggtatat
     4801 tttgttttct cgaatgtaat acaattgtcc cgaaccgtgt tgtatgaaaa gttttagctt
     4861 ctgtatagaa accataactt ttcttgacaa tcacagtcac aggagctatt ccattattgg
     4921 taggtggaga agtgaaaata tcatagttgg ccatagaaag tccaacttga tgaaacaata
     4981 gtttgattct ctggttttct tgctcacctc tgatattggt agctagttca gggaatatag
     5041 ccatatcggc atcaaaatgg ctaaaaattc gctcaatatg ttgtgcttct atttgattag
     5101 cgacgttcca agttactaat tttaaggttt tagtcttatt tgatgcagaa gaaagggttt
     5161 tatctgttcc aaataatagt aatatgagcg atagtaagag caaaatgcca gagtaaaacc
     5221 gtatttgttt atttttcttg taaaaagata ggaaaaaaaa ataagaaact taaaatagca
     5281 gccatagttg cagtagctaa ggggaaggca taaatctgtg ccaagatagg ttgggtagat
     5341 agccaaatgt taagattaga agatgggaaa aaagaataag ctatgatagg gataacaaag
     5401 agaatgaccg aaagggcaga gagaaatgtt ttaatcattg tgtgagtacc tcaatttgca
     5461 atggttgctc ggagctaatg tgactttgtg actttacttg atgatattct ttttattgtc
     5521 ttccttatag attaagtaca acccaagtaa aaaaagttca atttaagatt gaaatgatta
     5581 aaaggcacag aaatgcaaga caattatact acaaaaggca aacatttgac aatcgatagc
     5641 cgtcgcttaa tcgaacgatg gaaaaaagaa ggaaaatcaa atagagaaat tgcctctcta
     5701 cttggaaaag ctcctcaaac tatccacact gaaatcaagc gtgggacagt ccgacaatgt
     5761 cttggaaaag ggcgtttcaa agagatttat tctgccgact atgctcaaca gtcttatgaa
     5821 aacaatcgca agcgctcggt caagaaatca agcttgacca aggaactaaa ggaaaagctt
     5881 ctccactatc ataaccaaaa attttcgcct gaaatgatgg tgatggctaa aggggttaac
     5941 gtgggaattt caatcattta ctattggatt catcatggaa aattgggatt aagcaaacag
     6001 gatctgcttt accctagaaa aggaaaagcg cttaagaaac aagctagtcc caactttaaa
     6061 tccgctggtc aatctatcaa atcataattc gctcattttt tcaaaaatac atagacgtaa
     6121 aagaaagagg gttaagtgat gaagaatagt gctattggga gtaattggaa ggatgtccat
     6181 ctgagttctt tagcaaggag gaaatccttg aaagtgatat gcgagtggct attatgagcg
     6241 agttgattga ggctaggcat gaacaaggaa tcagtcaaaa aaagctggaa gaattcagtg
     6301 gagtaagcca gcctgtcata gctaggatgg aaacaggcaa gactagccct cagttggata
     6361 cagtcttaaa agtcttatcc agtttgggaa agacactagt agtagtctca ctcgaacaga
     6421 gaaagagttg attagagtag acttatactc aatgaaaatc agagagcaaa ctaggaaact
     6481 agccgctgac tgtacttgag tacggcaagg cgacgttgac gcggtttgaa tttgattttc
     6541 gaagagtatt atagtctttt ctaataacaa gccatagtca cttataagaa ttactaataa
     6601 catgttttag tattctaatt ggaatatagc tgtaaagtaa gaattacaaa ggatttgaga
     6661 cgtttgcttc aagtcttttt cttttgtccg aaacagagat atagtttcaa actatatcaa
     6721 accctaattt tttacaatta tacagacgct ttcttttctg ttaagatagt ttcaacaaca
     6781 aattttggag gacacatcat gtcaactaca atcatcggtt tccctcgttt gggggaattc
     6841 cgtgaattaa aatttacaac tgaaaaatac tttagaaaag aaatcttaga ggaagaactt
     6901 ttagcagcag caaaagactt gcgtgctaaa cactggaaca ttgtcaaaga aaaaggtatc
     6961 actgagattc catcaaatga cttttctcac tatgataact tcctagatgc agctttcctt
     7021 ttcaacgtag tacctgcatc cgttcaaaac ttgaacttgt ctgaccttga gcgctacttc
     7081 gctttgggac gtggttacca aggagaaaaa ggggacgttc gcgccctccc aatgaagaaa
     7141 tggtttaaca ctaactacca ctacatcgtt cctaaatttg aaaaagacac tcaagtcaaa
     7201 cttgcaggtc acaagatttt tgatgagttc caagaagcca aagaacttgg attgaacact
     7261 cgtcctgttc ttgtaggtcc cttcactttc cttcaattgt cagactttga agaaggcgtg
     7321 aaagcagacg acttcgtaga tagcttagtg gctacttacc aagaagtttt tgctaaattg
     7381 gctgaacttg gtgcgactcg catccaattg gatgaagcgg ctcttgtaaa agacttgaca
     7441 gctgaagaaa aagctctctt cttgaacctt tacaacaaac tcttggctga caaaaaaggt
     7501 cttgaagtct tgcttcaaac ttatttcggt gatgttcgtg acgtatacgc tgaccttgta
     7561 aacttgccag tagatgctat cggtcttgac tttgttgaag gtaagaaaac tcttgaactc
     7621 gttaaaggtg gcttcccagc tgacaagact ctctatgtag gtattgtcaa tggtaaaaac
     7681 atctggcgta acaactacga aaagagcttg gctgttcttg agcaaatccc agctgaaaac
     7741 atcgttttga caagctcttg ctcacttctt catgtgccat ttacaactgc taatgaagaa
     7801 tttgaaccag cactcttgaa ccactttgcc tttgcagttg aaaaattgga tgaaatccgt
     7861 gacttggatg ctatccgcaa tggtcaaggt tcagaagctc ttgcagccaa caaagaactc
     7921 tttgcgactg agcgtgttgg tgaaaatgct gaacttcgtg cgcgtatcgc tggcttgaca
     7981 gacgcagact acactcgttt gccagccttt gcagaacgtg aagctatcca agaagaagct
     8041 ttcaaacttc cagctcttcc aacaacaacg attggttcat tccctcaaac aaaagaagtt
     8101 cgtgccaaac gtttggctta ccgtaaaggt gaattgtctc aaaaagagta cgatgctttc
     8161 cttgctgaaa cgattgacga atggatcaag tggcaagaag atattgactt tgatgtcctt
     8221 gttcacggtg aatttgagcg taatgacatg gttgagtact tcggtcaaaa cttgtcagga
     8281 tacctcttct ctaaaaatgg atgggtacaa tcatacggta tgcgtggggt aaaaccacca
     8341 atcatctggg gtgatgtcac tcgtcttaac cctatcactg ttaaatggtc tagctatgca
     8401 caaagtcgta caaacaaacc tgttaaaggt atgttgactg gacctgttac catccttaac
     8461 tggtcattcc cacgtgaaga catctctatc aaggattcaa ctctccaaat cgcccttgct
     8521 atcaaggatg aagtacttga ccttgaagct gctggtgtga aaatcatcca aatcgacgag
     8581 gctgctcttc gtgaaaaatt gccactccgc cgtagcgact ggtacgaaga ctaccttgac
     8641 tgggctattc ctgccttccg cttggtacac tcaacagtag cgccagacac acaaatccac
     8701 actcacatgt gttactcaga atttacagat atcatcccag ctatcgataa catggatgca
     8761 gacgttattt cctttgaagc tagccgttca aaccttgaaa tcttggacga actcaaagcg
     8821 aaaaacttcc aaacagaagt gggacctggg gtttacgata tccactcacc tcgtgtacca
     8881 aatgaaggcg aaatcgacaa cacaatcgaa gccatccttg ctaaagtgcc aagcaagaaa
     8941 gtttggatca accctgactg tggtttgaaa acacgtggta tcccagaaac aaaagaaagc
     9001 ttgatccgcc ttgttgaagc agctaaagct gcgcgtgaga aattgtaaga ttggatttct
     9061 ctccatacag tggttgttca aaaagaaatc aactagaaaa ggttattaca tatgtcacgc
     9121 caaacaccgt cactctcatt tgaagtgttc cctccaaacc cagccgtggg taatgataac
     9181 attatttcag ccttgcagga tatgcaggaa ttggctccct attttatcag tgtaactgcc
     9241 agcaataata aatttaatat caaggaaacg acggttcgtt tggctgactt tatccaaaat
     9301 gatttggcga ttccgaccat tgcccacttg ccagctattt atctaactaa ggacaaggtt
     9361 gctgaaacta ttgctgactt ggacaaagtt ggggtgcaga aaatcttggc tcttcgtggg
     9421 gatattattc cagacgtgga accacaaaag gatttccgct atgcaaccga cttgattgag
     9481 tttatcaagg aacaaacccc tcactttgat attattggag cttgctatcc agaagggcat
     9541 ccagattcgc caaatcagat ttcagatatt caaaatctta agaagaaagt ggatgcaggc
     9601 tgttcgagcc tcgtaactca gctcttcttt gacaatgagc gcttctatga ttttcaggac
     9661 aagtgtatct tggctgggat tgatgttccc attcatgcag gaattatgcc aattctgaat
     9721 cgaaatcagg ctctccgact cttgaagact tgtgagaata tccatcttcc acgcaaattt
     9781 aaagccatct tagacaagta tgagcatgac cctgagtcgc tcagagcagc aggacttgcc
     9841 tatgcagtgg accaaatcgt ggacttggta actcaggatg ttgccggtgt gcatctctat
     9901 acgatgaata atgctgatac agcaaaatac atccatcaag caacccatgc cttgtttaat
     9961 caccagtctc taggataata aaagcaaacc attcttctca ggtgagggga atggttcctt
    10021 tttaatggta aagacctcac tttttaagaa aaatatgata aaataaattc tgtacgtact
    10081 tgatacaaag atgaaggtat taaataatac aatgcattta aactgtaaga tttaacgaca
    10141 cgagtgagtc aaatcgatgt tatttgacga gagagttagt aaagcattta gctaatcgcc
    10201 cgatagcgat tagcgtgaaa gctttagata ttttagtatt aaacctttct aatgatgtat
    10261 cttgaaaact gaacttgcct acaactctgt ggaaaaagat aaatctccct aggagcagtc
    10321 gctccgtcgt tcgatttcct at
//
LOCUS       DAVKDZ010000071         9505 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000071 DAVKDZ010000000
VERSION     DAVKDZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9505
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.072"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(<1..1450)
                     /gene="pfbA"
                     /locus_tag="VWZ30_001720"
     CDS             complement(<1..1450)
                     /gene="pfbA"
                     /locus_tag="VWZ30_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEU9335406.1"
                     /translation="MKYFVPNEVFSIRKLKVGTCSVLLAISILGSQGILSDEVVTSSS
                     PMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNSISSNGTIRSNS
                     QLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAI
                     QDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIV
                     FMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNS
                     NNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEP
                     LTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKIL
                     NNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVNAYSYKNTKDLL
                     DLNKQVVIAENIFNIADPKTKAIRVAKDSAEYL"
     gene            complement(1706..1993)
                     /locus_tag="VWZ30_001721"
     CDS             complement(1706..1993)
                     /locus_tag="VWZ30_001721"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335407.1"
                     /translation="MSDIKVNIPGECLYDKVFVLSFIIYNISINLNIVNGIFYIQAKK
                     DSITFEWKAKEQTRKLAIDSSKPCFEVVDIVKGNKNRTNRSGQSNRFLTMF"
     gene            complement(2614..3840)
                     /locus_tag="VWZ30_001722"
     CDS             complement(2614..3840)
                     /locus_tag="VWZ30_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEU9335408.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKIVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(3883..4575)
                     /locus_tag="VWZ30_001723"
     CDS             complement(3883..4575)
                     /locus_tag="VWZ30_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9335409.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLAFVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(4748..6499)
                     /locus_tag="VWZ30_001724"
     CDS             complement(4748..6499)
                     /locus_tag="VWZ30_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9335410.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMTYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMHFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMADTL"
     gene            complement(6489..8234)
                     /locus_tag="VWZ30_001725"
     CDS             complement(6489..8234)
                     /locus_tag="VWZ30_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9335411.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLVFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGTHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(8440..8736)
                     /locus_tag="VWZ30_001726"
     CDS             complement(8440..8736)
                     /locus_tag="VWZ30_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU9335412.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYK"
     gene            complement(8889..9344)
                     /locus_tag="VWZ30_001727"
                     /pseudo
     CDS             complement(8889..9344)
                     /locus_tag="VWZ30_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
BASE COUNT         2897 a         2010 c         1833 g         2765 t
ORIGIN      
        1 ctaaatattc tgcactatct tttgcaactc gtatcgcttt tgttttagga tcggcaatat
       61 taaatatatt ttcggcgata accacctgtt tatttaaatc taataggtct ttagtgtttt
      121 tatagctata agcatttact aaagctgctc cgctttctcg ataatgtaca ctctctcctt
      181 taactttctt atcaaagcga tttcctttga ttaatacatc agtgaatcct gtaaaacgta
      241 cacctgcata catcatgtta tcaaaatgat tatttaaaat tttaatatta gaggggttct
      301 gtgtcgacaa tgtttgatag tgtgtgccaa ttgctgttac taattcccca gatttatcac
      361 ttttgccaaa ataggaattt tgaatagtca cattttcaga ttttttccca tcatcattca
      421 aggcataagg aaaacctttt ctagttaatg gttcaatctg aatgctctcc ttacttatga
      481 tttgcccatc cttcatcgtt ttgggtaagg cttgcccaag aaaacgagaa ttatcaacta
      541 atacattttt cgaacctgca atttgaatag catgcccttg ataactatcc ttgaatgtta
      601 cattttttat agttacgtta tttgaattcc caatagcaaa tgcacctgaa gaatttataa
      661 gtggtagatt ttttgcttta gttccttctt cattcaaagc accgttcata tcaatcgtac
      721 caccagaata actaatatct tctgttgggc cccattctac ttgcgcacca tcatccgtaa
      781 ataaacctgt cataaaaaca atggaagggt gattcttaat atttatacca tttagaattg
      841 tagctttctc attcaattct aagtgtgtat gactttttaa aaaaacaatt tcttttacta
      901 aataagttcc ttcaggaaaa tatacatttc ctccacctag cccttgagct gcagcatcta
      961 ttgcatcttg aattgcttgt cgatcatcat gaatcccatc acctactgca ccataatctt
     1021 ttacacttaa agcagtatct tcaaattctt ttttgataat tctgtcactt ataacatctt
     1081 ccttataact cttattttca ttagtagatg ttactgtaga ttcaactgtt ctgttgtcta
     1141 attgtgaatt ggaacgaata gtaccattag agctgatgct attaacactt aacgaattat
     1201 ctaaaccatt agtggttgaa ttagaggcaa tcatttcagc agaacgattc tgattaacag
     1261 ttggtgaatt atctaaatca ttagtaattg cattagaaga ctcttttgta gccatcggtg
     1321 aagaactagt aacaacttca tccgataaaa taccttggct tcccaaaatt gaaattgcca
     1381 atagtaccga gcaagtcccc acctttaatt tacgaatact gaatacctca ttaggaacaa
     1441 aatatttcat cagaacctcc aattttttta ctatttttaa caattgatta ttacttttct
     1501 taaaatagat agaatgacac cgctgtcatt atctttatca tatattttcc aaattgaatt
     1561 gtcaaggaat aacacaagaa aatattaaaa tttatactct tcggaaatct cttcaaacta
     1621 ggtcagtgcc gccttgccct aagtctaggt tactgacttg gtcagttcta tagtagactg
     1681 aaactataat agtacacctc tacttctaaa acattgttag aaatcgattt gactgtcccg
     1741 atcgatttgt cctattctta tttcctttta ctatatctac aacctcaaag cagggttttg
     1801 agctatctat ggccaacttc ctagtttgct ctttagcctt ccattcaaaa gtgatgctat
     1861 ctttcttagc ttgaatataa aatatcccat ttactatatt taaatttatt gatatgttgt
     1921 aaattataaa tgaaagaaca aataccttgt catacaagca ttcgccaggg atatttacct
     1981 taatatcgct catatggcgg attgaaacta aaacactaca atatatttct taaatagacc
     2041 ttctataaaa gctagattta acctcgataa aaatagatgc agtgcttcta aaatactgga
     2101 atagggcata taggtaatcg tagacaagtg ttttatagat tgtctcgcag gagatctaat
     2161 gatgtatagc aagcatcact aaacatatgg gatattcgta ttattataat atcaacatgc
     2221 tataaattct atcatgtaaa agaatgacta gaacatgaaa gacaggcaca aaactttgaa
     2281 caatattctg aaaacaaaaa aaagacgcga gtttccacaa tctcactcat ggaaactcac
     2341 gtctttcaaa aaatagggtg aattgaaaac tagaacaaga cactagattt tctaaatgat
     2401 gtctagtaag gattctaaat gctacaccaa attgctcaca tcttatttca aaccacggga
     2461 ctcccacaag aaaggaaaat cctataaaaa ttctctctct gaggagaaag gagcctcaac
     2521 tacaggtgta aactattatc aattgaaaac aacagttttt acactcaatt agacttttta
     2581 aggacaggat cactttcgct tgactgtagt ttgtcatttt tttgataaag ttagcacttt
     2641 ttcagaaact gttttgaaag tctcaatgat atagtctact tcttcatcgc ttaatttagt
     2701 atgaagaggg agggtaattt cattctcaaa gaaggcatag gccttaggat agttcgtcat
     2761 atcaaatcca agattcttat aggctgtcaa gagaggaagc ggtttgtagt gaacattact
     2821 tgcaattcct gctttagcca attcttggat gatgaggttg cgttcttcta ggcttgctcc
     2881 ttctacacgg gtgatgtaga ggtggcgtga agattcgaca gtttcagtct tgtgtgccaa
     2941 aggatggatg cgagaacctg caaaaccact atcatagcgg tccacaatgt ccttacggcg
     3001 ttgtaacaaa cttggatagc ggtccaattg taccaaacca agtgaagcca tgatatcggt
     3061 catgttgcac ttataggctg gtgtaacgat atcgtattcc catgacccca gttgcatctt
     3121 ggcaagagca tccttagttt gcccgtgaag ggaaaggatt tggaattcct tgtacatctc
     3181 ttcgtcatca atcactggat tggctttcca agtcgcactt ccaccttctg ccgttgtaaa
     3241 gttcttaacg gcatggaatg agaaggaagt aaagtcagcg atagaaccag aaggttgtcc
     3301 tttataagta gatcccaaag cgtgggcact atcagagaca atgacaatac ggttaaaggc
     3361 cttttgccac ttgcttgaag cggtaaagaa gtcacgtttt ttctccacga cttggaacaa
     3421 acggtcataa tcgcaaacaa tccctgcgag ctctactggg ataatcacct tagttttctc
     3481 agtgatagct tgctcaagca ggtcatagtc catctcaaac gtatctgctt ggatatccac
     3541 catgacaggg gttgctccca cgtgcgtaat gacactacat gaagccgtat aggtcatggc
     3601 tggaacgatg acttcatcac caggtcccac ttccaaaacg cgtaaaatca actccagagc
     3661 agctgtcgca gagttgagac aaacaatctt aggtgtctgt gtgtaaagag acaagcggcg
     3721 ctccagttct tttgttttag gacctgttgt gatccaacca gaacgcaggg tatccgctac
     3781 ttcagcaatt tctgcttctg tgatatcagg cggtgaaaat ggaatattgt aatttggcat
     3841 tgatttgctc cttttatctg tactcatttt cttatgacta ctttatttta gtacctcaaa
     3901 cacggtttga aacatgattt tgatgtcccc aaagaaacta aactctcgga gataggcgag
     3961 gttatagcgc atcttttcag gaagaacatg ctccacatag gcctgatcaa ctgacagacc
     4021 tttctccgtc atttgactga tgatggtgtc ctcatccttg tagttgatgc tggctggaga
     4081 ggtaattcct gcttgcaaga gcaaggttgc catcatttca gggctatact gctctgtata
     4141 acgtggcact tcaggtcgtg taccgacaaa ggacatctca cctttaagga cattgaccaa
     4201 ctgaggcagt tcgtccaaac ggacacgtcg gatgaaattt ccaaccttgg taatacggct
     4261 atcgttagca gaagtcacca gacttccttt tttatccgca tccgtcacca tggtacggaa
     4321 cttccaaatc ttgaaccgac ggttgtactg ggtcacacgc tcttgcttgt aaatcactgg
     4381 ccccttgcta tccaacttga tccaaatgct caagatgaga aagatgggag aggtcagaac
     4441 cagtaagaca aaggctagaa cccagtccaa gcaacgcttg aaaatcagcg aacccttcct
     4501 tttagagaca agctggtaat aagactcaac ctcgcttgat ttcatttcca caggcaagtc
     4561 ttcccatttc agcatgctgt tctcctttgt ttttattata ctatttgtca acatttttca
     4621 ttataccaca aaatggaaaa ggcggtgaaa gaaatctgta atttgaagga tttccttcta
     4681 gtactcaatg aaaatcaaag tacagctttg agattgcaga taaagctgac atggtttaaa
     4741 gagattttca aagagtatcg gccatggccc ctgcctgcaa actatacatc ttgtgatagg
     4801 ttcctcccag agtcaagagt tcctcatggg ttccactctc gataatgcgt cccttatcca
     4861 agacatagat gcagttggcg tcttgaatag tagaaaggcg gtgagcgata gcaatagttg
     4921 ttcggccctg tctcatcttc gccagagaag cttgaaccaa gctttctgtt tcagagtcaa
     4981 tattggctgt cgcttcatcc aaaatcagga ttttaggctg gctggcgact gttctagcaa
     5041 aggcaaggag ctggcgttgc ccagtagaga agctcgaacc acgctcggaa acaggggagt
     5101 cgtacccctg aggaagttct tgaataaagg aatctgcatc cacaaaggct gccgcagcct
     5161 gaacctgctc atcactgatt tcttggtaca tggcgatatt ggacttaata gttccatgat
     5221 agaggaaggg ttcctgtaaa accaaaccga tgttttttct cagctcttct tgactgaaat
     5281 ccctgatatc cacatcatcc aagagaactc tccctgactg gaattcataa aagtgcatga
     5341 ggacattgat aatagacgat ttccctgaac ctgtatgacc tacaaaggca atggtttcac
     5401 ccttattaac agagaaagaa atgtcatcca gaatcggatg tttaccgtca tacgagaaac
     5461 acacatgttc aaaacggata ttgccttctt tgactttggc ttgcccattt tcttgaagag
     5521 gctcataggt cctctcatcg attaaggcaa agacacggcc tgcagaaacc atagacgttt
     5581 gcagtgttga aaagttttgc gtcacctcaa tcaaagggtc aaagaggcgg ttgatgtact
     5641 ggataaaagc atacatggtc ccggccgtta tcccgataga aagaccacga tagccaaaat
     5701 aggtcatcaa gacagcataa cctagaagtt tcaacaaact catggcaggt ctcaaaaaga
     5761 gggcatccaa ggctacagaa cggttggcat aggccaagtg ctcttggttg atttcatcaa
     5821 attctgcctg caggcgcttc tcttgattaa aagcctgaat aatcctgatt ccctcgatat
     5881 tctctgccag cttactattg atatctgaca agagacttct ggttttctcg atgattttca
     5941 ctgacttttt tcgatagaga ttgaccaaaa ggaaaatcaa aggaagaaag agcaagacta
     6001 aagccgtcaa acgaaaatcc agcaccaaca tggtataaag ggttgtcaga aagataaaaa
     6061 ctgctgagat aaagctggat aaaatcccag aaaacatatc actaatcgtc tcggtatcgt
     6121 tggtcaaacg agaaacgata gaacctgctg gcgtcttgtc aaagtaagac atgcccagtt
     6181 tctccatatt agcaaaggca tctcgacgaa tatccctaac aatactataa gacacgcgcg
     6241 caaagagaag attgcccaca tactgaacta cagtttgtag gatgtagaga ccatagtaga
     6301 ccagcaaaac ggtaacggct agttggttaa gattgctgag atactggtcg ataaagtggg
     6361 aagccacgag gggtatgaca cttttaatga ccgtcgtcgc taggagaaaa ctgagtgcca
     6421 aaaaggtcag gagtccataa ggcttgagat aagacatcaa gcgcttcaat acagcccatt
     6481 gttcttgttt attctgcatc ttcttctcct ttcatttcca actgctgaga ctggtaggtt
     6541 tgggcatacc agccatccat cgctagcaag tcttcgtgcg tgcctcgttc gataatttga
     6601 ccattttgta gaactaaaat aaaatctgca tggacaacag cactgaggcg atgggcagtg
     6661 ataatggttg tcttgtcctt tcgcgtctcc ttgaggttgt cgataatcgc atactctgtc
     6721 ttggcatcta cggcggataa ggaatcatcc aaaatcaaga tatcagggtc taaaatcata
     6781 gcccgactca tagccaaccg ttgcttttga ccaccagaaa gactgactcc tttttcacca
     6841 atcagcgtat caaatccttg aggcatgtct acaatatctt ggtaaacccg ggctagctta
     6901 gtagcttcct cgaccgctga aaggggcaag ttaggattgc caaagcggat attgtctagg
     6961 attgaagtcg caaaaagaaa ctggtcctga ggaacatagc ccatgagact gcgaaggtct
     7021 gtcagacgat agtcccgaat atcgtgaccg tttagataaa tggcaccctt atccacatcg
     7081 tattcacgca agaggagctt gattaaggac gttttcccag agcctgtctg cccaaccaag
     7141 cccagtgttt gcccttttgc caaactaaag tgaatatccg tcagtgtttc ctcattttca
     7201 aaagcaaagc tgtcaatggc atactccaaa cgcccatttt caataccatc cagaggaaac
     7261 tcagggtctt gtacaggaga ttcctgagac aaaagatttt caatccgctg gtaagaaacc
     7321 ttccctcgct gagtagtatt aaagaggaag ccgatggcca taagaggcca gaccagcata
     7381 tccaaatagc tgataaaggt gactagattc ccaactgtaa tctgcccttc ctgaaccatc
     7441 aaggagccaa ccaaaagcgt taaaacatag gacgaaccaa caaacacgag aaccataggg
     7501 tcaaagagac tatcatattt catggtttgc aggttctttt ggaaggttaa ttcattgact
     7561 gcctgaaaag acttcaactc gtctgcctga taaccgaaag acttggtcac tttgatacct
     7621 gatacggact cctgtacctt gttattgagt tcagaaaaag cagcttggga ttcgccaaag
     7681 gccttatgag tctttctccc taggcgacta gtcgcatagg ccatgaaagg tagggggaga
     7741 atggcaacaa gagtcatctg ccatgagatg ctaaagagca tggtcaacaa agtcaccaga
     7801 gccgtgatag aggcatccac cgcagacatg acaccgccac ctgctaaacg agtcaaggca
     7861 ttgatatcat tggttgcgtg tgccatcaga tcacccgtcc gataggtttg ataaaaggct
     7921 gatgacattt ttgtgaaatg cttaaacaag cgagaccgca tgatctgtcc caagcaataa
     7981 gaggtcccaa ggatatacat acgccacaca tagcgcaaat agtacatacc aaaggctgca
     8041 agtagcaagt aaaataggct aagaaggagg tcctgctgag ttaattgccc cgatgtgatg
     8101 gcatcaatga ctcgccccat aaccatagga ggaatgagat tgaggacgga aaccaagacc
     8161 agggccacaa tcccgactag ataacggcgt ttttctaact tgaaaaacca ccagagtttt
     8221 tgaataatgg acataaaatc cctttctgat tgcaaatgga aacctgaggc caatactcaa
     8281 tgaaaatcaa agagcaaact aggaaactag ccgcaggctg ctcaaagcac tgctttgagg
     8341 ttgtagatag aactgacgaa gtcagtaacc tacatacggc aaggcgacgt tgacgcggtt
     8401 tgaagagatt ttcgaagagt acaagacccc aggtttttct tatttataag ttacgactgt
     8461 aacagcaccc ttgtcatatt cagcaataaa gatattggct acattgtcat gcccttgttt
     8521 actgaggtta tcaagcaacc actcttcgct acgaccaatc gattccaaga catctacttg
     8581 aatcacaccg tcagtcacaa ctggatactt aggattttca tctcccattt gcacaacgat
     8641 gagttgccca ttttgctctt gcacagctcg tttgacttgc ttcatctgga aaatcccttg
     8701 gctacgaagt ttgagggcta catccgatgc agacaaacca actgaacgac aggcttatgg
     8761 gtcaatattc ccatttttga taaggagagt tggcttacca tcaatcaaac gtttcacaaa
     8821 gtgaacattg ttgttgagct atttgagagt caagaccagt ttgattaaaa tttcgatata
     8881 attaagtgtc atttgttcac atccaccagt tcaattgcgt ctgtcttata caattcgact
     8941 ttctctaaca ggtacttgtc tggctcactt ccgtttaggg cacgataata gcgttctccc
     9001 accttgataa gtgcgccatc tgtgtttgtg gaagtattaa catagacttc tgacttgtct
     9061 actcccaaat ctttggaaac aacctcgatg aaatgaagtg aagtttgaaa tttattgtta
     9121 gaaacttgat tgttttgata agtcgtgatg ctgaccaaaa gcagagctaa ataaggccag
     9181 agctgaaatc atgaccaact cacgaaattc ggttcccttt ttatcatggt ataccttaaa
     9241 agcaaaaaaa ctgtcacagc taatagaaca atcccaaagc caaccatgat accattttgc
     9301 tgaccgattt ggctgagtac atagtcataa gagtagaatt tcatgtgttt tcactccctt
     9361 tcataaaatc attcttaatt ataaacgaaa gagagtaatt tttcaaacta tacgttgggg
     9421 aaatagatct ttttttggta taataaaatc tataatctga atgaaaaagg taactttatg
     9481 aaacttatct catggaatat tgatt
//
LOCUS       DAVKDZ010000072         9463 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000072 DAVKDZ010000000
VERSION     DAVKDZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9463
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.073"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            11..733
                     /locus_tag="VWZ30_001728"
     CDS             11..733
                     /locus_tag="VWZ30_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9335413.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            730..1578
                     /locus_tag="VWZ30_001729"
     CDS             730..1578
                     /locus_tag="VWZ30_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9335414.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            1610..2539
                     /gene="psaA"
                     /locus_tag="VWZ30_001730"
     CDS             1610..2539
                     /gene="psaA"
                     /locus_tag="VWZ30_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEU9335415.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            2662..3153
                     /gene="tpx"
                     /locus_tag="VWZ30_001731"
     CDS             2662..3153
                     /gene="tpx"
                     /locus_tag="VWZ30_001731"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU9335416.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(3249..5957)
                     /locus_tag="VWZ30_001732"
     CDS             complement(3249..5957)
                     /locus_tag="VWZ30_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9335417.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALTTHLQDQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLRLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQAQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVATVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(5959..6660)
                     /locus_tag="VWZ30_001733"
     CDS             complement(5959..6660)
                     /locus_tag="VWZ30_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9335418.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            complement(6910..9321)
                     /locus_tag="VWZ30_001734"
     CDS             complement(6910..9321)
                     /locus_tag="VWZ30_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9335419.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLVKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     RKAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKISTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
BASE COUNT         2757 a         2193 c         1800 g         2713 t
ORIGIN      
        1 atacaagttt atgatacgta tcgaaaacct cagtgtctcc tacaaagaaa cgttggcact
       61 taaggatatt tcactagtgc tccatggacc aacaattacc ggcatcattg gtccaaacgg
      121 cgctgggaaa tcaacactat taaaaggtat gctgggaatt atcccacatc aaggtcaggc
      181 atttctcgat gacaaggaag ttaaaaaatc cttacaccga attgcctatg tcgaacaaaa
      241 aatcaatatc gactacaact ttcccatcaa ggtcaaggaa tgcgtctcgt taggactatt
      301 tccctctatt cctctctttc gaagtttaaa ggctaaacat tggaagaaag tgcaagaggc
      361 ccttgaaatc gtcggcctag ctgactacgc tgaacgtcaa attagtcaac tgtctggagg
      421 tcaattccag cgggtcttga ttgccagatg tttggtgcag gaagccgact atatcctctt
      481 ggatgaaccc tttgctggga ttgactctgt cagtgaggaa atcatcatga atacgctgag
      541 agatttgaaa aaagctggga agacggttct catcgttcac cacgacctca gcaagattcc
      601 ccactacttc gatcaagtct tacttgtcaa tcgagaagtg attgcctttg gtccaacaaa
      661 agaaactttt accgaaacca atctaaaaga agcttacggt aatcaactct ttttcaatgg
      721 aggtgaccta tgattgcaga atttatcgat ggattgcaaa aattccattt cctacaaaat
      781 gccttgataa cagctattgt cgtcgggatt gtagctggag ccgtgggatg tttcattatt
      841 ctacgcggga tgtcactcat gggagatgcc atttcacatg ctgtcttacc aggtgtagcc
      901 ctctccttca tcttgggcct tgacttcttt atcggagcca ttgtctttgg attgctagct
      961 gccatcatca ttacctacat caaaggaaac tcgattatca aaagcgatac cgccatcggc
     1021 attacctttt cttctttctt agccctcggt atcatcttga ttggtgtcgc taaaagttca
     1081 actgaccttt tccatatcct ttttggtaat atcctggccg tccaagatac ggatatgttt
     1141 attactatgg gtgtgggggc agccatcctc ttgttaatct ggattttctt caagcaactc
     1201 ttgataactt cctttgatga actcttggct aaagccatgg gaatgcctgt caatttctat
     1261 cactaccttc tcatggtact cctgactctc gtgtctgtga cagccatgca aagtgtcgga
     1321 actatcctga ttgtagccat gctgattacc ccagctgcaa ctgcttatct gtatgctaat
     1381 agtctgaaaa gtatgatttt cctttcctca accttcggag ctactgcttc agttttggga
     1441 ctctttattg gctatagttt taatgttgcg gcaggttcta gtatcgtgct tacagctgct
     1501 agtttctttc tcattagctt ctttatcgct cccaaacaac gatatttgaa actgaaaaat
     1561 aaacatttgt taaaataagg ggcaaagccc taataaattg gaggatctaa tgaaaaaatt
     1621 aggtacatta ctcgttctct ttctttctgc aatcattctt gtagcatgtg ctagcggaaa
     1681 aaaagataca acttctggtc aaaaactaaa agttgttgct acaaactcaa tcatcgctga
     1741 tattactaaa aatattgctg gtgacaaaat tgaccttcat agtatcgttc cgattgggca
     1801 agacccacac gaatacgaac cacttcctga agacgttaag aaaacttctg aggctgattt
     1861 gattttctat aacggtatca accttgaaac aggtggcaat gcttggttta caaaattggt
     1921 agaaaatgcc aagaaaactg aaaacaaaga ctacttcgca gtcagcgacg gcgttgatgt
     1981 tatctacctt gaaggtcaaa atgaaaaagg aaaagaagac ccacacgctt ggcttaacct
     2041 tgaaaacggt attatttttg ctaaaaatat cgccaaacaa ttgagcgcca aagaccctaa
     2101 caataaagaa ttctatgaaa aaaatctcaa agaatatact gataagttag acaaacttga
     2161 taaagaaagt aaggataaat ttaataagat ccctgctgaa aagaaactca ttgtaaccag
     2221 cgaaggagca ttcaaatact tctctaaagc ctatggtgtc ccaagtgcct acatctggga
     2281 aatcaatact gaagaagaag gaactcctga acaaatcaag accttggttg aaaaacttcg
     2341 ccaaacaaaa gttccatcac tctttgtaga atcaagtgtg gatgaccgtc caatgaaaac
     2401 tgtttctcaa gacacaaaca tcccaatcta cgcacaaatc tttactgact ctatcgcaga
     2461 acaaggtaaa gaaggcgaca gctactacag catgatgaaa tacaaccttg acaagattgc
     2521 tgaaggattg gcaaaataag cctctgaaaa acgtcattct catgtgagct ggcgtttttt
     2581 ctatgcacac atttccggtc aaatcattgg aaaattctga ctgttttaga tacaatggaa
     2641 gaaaaaagat tggagtatcc tatggtaact tttctcggaa atcctgtgag ctttacaggt
     2701 aaacaactac aagtcggcga caaggcgctt gatttttctc ttactacaac agatctttct
     2761 aaaaaatctc tggctgattt tgatggcaag aaaaaagtct tgagtgtcgt tccttctatc
     2821 gatacaggta tctgctcaac tcaaacacgt cgttttaatg aagaattggc tggactggac
     2881 aacacggtcg tattgactgt ttcaatggac ctaccttttg ctcaaaaacg ttggtgcggt
     2941 gctgaaggcc ttgacaatgc cattatgctt tcagactact ttgaccattc tttcgggcgc
     3001 gattatgccc tcttgatcaa cgaatggcac ctattagcac gcgcagtctt tgtcctcgat
     3061 actgacaata cgattcgcta cgttgaatac gtggataata tcaattctga gccaaacttc
     3121 gaagccgcaa ttgcagctgc taaagcccta tagaaaaaat cctctgtcac aaagagctcc
     3181 ctgctctttg tgacagagga tttttgaata ttagtaaata gaagttcaat cagctaaaaa
     3241 taactacctt actctacaga tttcagggct tctaacatat caacctttct cagataataa
     3301 ttgacgaaga aaccaagcaa ggtcaaaatg atgcttactg ttgccactgg gattacatag
     3361 acttcccagc ctacctgcgg ataaaagaga atagtcgcag gcgaaatcat ttgaatcaaa
     3421 aattggtgta aatagaaacc agctaccaga ccaagtacga ttcccacaag ggacagcaca
     3481 atcgtctcac ggtaaatgta gagggtgact tcattattat gaaaaccgag aaccttgata
     3541 gtggagagtt cacggattct ctcagctacg ttgatattgg tcagattgta aaggatgaca
     3601 atagctaata gaaccgatac gatgaccaag atggtcatgg tctgattgag tgagctagcg
     3661 atagagtcga agagtcgaat ggctgaagca ttttggacaa cgctggacac cgcagattga
     3721 ttcataagca agcccgcctg actttcgata ctagttgcac tggtatccct taatgagacc
     3781 agataagtgt tggcttgggg tagctgtccg taaagttgct catagctagc ctgactcata
     3841 taaataaagt gaccaacgta gttctcagta atagcagcga cctttagttc cttaccttca
     3901 atttctaaag tctgcccaac cttgacacct gccagctggg cgagtttagc tgtaataacg
     3961 atgccatctt ttaatgtcag ctcctgctga tgatgttgaa gatggataaa gggagtcaaa
     4021 tcttccttct ctatcatcat aagagtaatg ttttgaagac cagccttgcc tttgaaatcc
     4081 ttgtctagcg ttttagaata gattttctgg taggctagta tctcctgccc tttcaacact
     4141 tctgctagct ctaccttgtc ctgattggtc gcactaggat tttcagagac aagcatctga
     4201 tactgttgga tttgttgaaa ctgtttagac ggaactcctg ctacagaaga ttggattccc
     4261 aaacctgcaa agagcagagc tacagaacct gccacaccaa agattgtcat caacattctc
     4321 tgcttataac gaaagatgtt gcgggctgtt accttatgag taaaactgag acgacgccag
     4381 ataaaaccga tacgctccaa taagatttta gctcctttga caggaggttt aggaagtaga
     4441 agctgggctg cttcggcatg aagttccctc caagccacca gataggctgg taacacactc
     4501 gccaaccagc tcaagacaag agctagtaag ctataggtcc aatagaactg aatctgggtt
     4561 tctcccacca ccatgccttt tgtaatgaca cttgaaatta cactggctag caaataatga
     4621 ccaagtatac tacctagagc cgttccgaca gtcccagcta ctagtccata aaggagaaac
     4681 ttggcgataa tatccttact acggtaaccc aaggccttaa aaatccctgc atgagttcgc
     4741 tcttcgtcta caaagcgagt catggtcgta aaggtcacca tggctgctac ggcataaagt
     4801 accacaggaa agatattgcc cactgctcga atactggatg aagcattgct atacataaga
     4861 tagccctgac cacctggcat ggtctgacga ttataaacct gataccttgg ctccgccaaa
     4921 tcatccaaga cttgctgatg tttttctaat ttttcctttt cttgggctag attttgttca
     4981 gcttgcttta gtttgtcctc ttccttgccc aattcctgct tggcttgggt aagttgagca
     5041 ctagcctgtt ctctctgagc ttgaggaaac aaggctagtt gactttcttg agcctgtata
     5101 cgagcttgag cagctgctaa acgacgcttg ccttcctgca aattagtctg agccttgtca
     5161 agggtctctt gccccttgtc tagagactct tgtccttctt ttttcaaaag tcgcagacgt
     5221 accttgccat tatctgataa aatttgttca agctcttcct gatgttgctt ggatttttct
     5281 tcataagctg atgaaaaggc atttaaacct gctaaatctt gatatttcaa acgagctata
     5341 ttgtagactt tctgatcaaa ttgactaggt aaaatcaccc cataggctgt cagagtccca
     5401 cttccacttc ctgcgtagcc catatctcgc tgggagagga tttcagccga atccacaaaa
     5461 ccagtaatgg tataagtatg gtcttttaaa gaggaatgac cctcttcttt ttctttaaaa
     5521 ctaatctcct gtcccacgct gtattggtct tgcaaatgag tggtcaaagc gatttccttg
     5581 tctgactgag gaagtcgtcc ctttcttagc tgaaaggttg aaattcgctc tggtttggag
     5641 tacagccgaa tggcatcctg cccattatcc atagtcacat ctgtcaaata gccaaactcg
     5701 acctctgcgc cctccgtctg ttttagttct tcttggtctg cttgatccaa gccatagtta
     5761 gacatgactg ccaaatctaa ggtttgagca gttgttaaat aagcattagc tgtcgcctcc
     5821 atgttgggac tggttacttt gaggcctact aaggctagag atcccaacat catcaggatc
     5881 aagatggata aaaaacgccc cttggagcct gtgaaggact gaattaagtc cttccaataa
     5941 gtttttcgct tgatcatgct agtactccaa actgtcaata tcctgaggat gctggttgag
     6001 caccacatcc ttgacactgg catcgtgcat ttgaatcacg cgatcagcaa tgggcgccaa
     6061 agctccatta tgagtcacga tgatcaccgt cgctcccttt tgacgagaca tgtcttggag
     6121 aattttcaaa acctgcttgc ccgtctgata atccaaggct ccagtcggtt catcacaaag
     6181 gagaatttta ggatttttgg ctaccgcgcg tgcaatggag actcgctgtt gctcccctcc
     6241 agaaagctgg gctggaaagt tattgagacg atgagccaga cctacatctg tcaagacctg
     6301 atcaggattc aaggcatctg tcacaatttc agaagccagt tccacatttt ccttagctgt
     6361 cagattagaa actagattat aaaactgaaa aacaaacccc acatcatttc tacggtaatt
     6421 ggtgcgctgg tgggaactat aatccgcaat attaacacca tcaatccaga tttccccttc
     6481 atcattggta tccattcccc caagaaggtt aagaactgtt gacttgcctg cacctgaagc
     6541 accaaggata ataaccagct cccccttttc aatctcaaaa ttcacatcac gattggccac
     6601 aatctccgtg tccccaacct gataacgctt gtaacagtgt ttcatctcaa tataagccat
     6661 cagatccacc ctctatcaaa caaattatac tcctcgaaaa tctctcttca aaccacgtca
     6721 gcttccatct acaacctcaa aacactgttt tgagcaacct gcggctagct tcctagtttg
     6781 tgctctttga ttttcattga gtattacttc ctattatcat tataacgctt tttcaactag
     6841 ttggaaactg cctatattct taaatcctta ttaaaaggaa gtttcaaatt actgccttat
     6901 aacatcttct taaaaataaa attgaacaag atcaccttct gctgttgcca ccgaaataca
     6961 atctttcccc atgcatttcg cttttatttt ttcttgattc attttaatca cactcttctc
     7021 aatatctgga taagaaactc gcaaatctcc tccactcctt gataaaatgg tcaactgtaa
     7081 gagtttttta tcttcccagc tcatgctcac ttcaaaatgt ccacgtgcca ttaagcctga
     7141 aacagaacct gttgaccaag catcaggtag ggcagctaga ggtaccagat aagctgcatg
     7201 agactggagt aacatttctg ccatgccact agtagcacca aaattaccat ctatctgaaa
     7261 aggaggatgg ctacaccaaa gatttggcaa ggtggatatt tttaactgct ctgccaataa
     7321 tttatgagct cgattgccat ctcccaaacg cgcccagaga ttgatcttat tagccttgga
     7381 ccagcctgtg ccgccatctc cacgatcatt gaggctagca cgtgccgctt caatatactc
     7441 ttgtcccttg tagctaaaga gattgccagg atagagtccc actagatggg aagcgtgccg
     7501 gtgctgggcc tccactttct cattttgaaa atactgctct tcctcctcat accactccct
     7561 gattcgacca gattgagtga tttgcaaagg atttagtaaa tcagacttct ccttaacctc
     7621 agtcaacaag tcctcatcca gtcccaattc ctgagcagcc tgaataaaat catgaaataa
     7681 ctgccaaatc agagattggt cataagtatt gccaatcgaa atcggcccat gttctgggga
     7741 ataagacgga gaagacaccc aacgctgcgc ctgctgatcc ttatgtaaaa aggcattcca
     7801 aaaacgaacc gtttccctca acatgggata aattttctct ctgagatagt cttggtccct
     7861 ataaaatgaa taggcttcat aaacggtttg catcatccac gcattggcag ctggtgacca
     7921 accccaatag taatcccaac caggtgccgt ccaaccaaag ggagtcgctt gagtatgaac
     7981 caaccaacca ttctcctcac ctttctgaga gacgattcct gcatacttta cagccgctag
     8041 acgaccatag acacgcaaat catctacata gttgatgact ggaaagaccg tctctaggag
     8101 attggtaaca taggctggcc aataattcat ctgcagattg acatttaagt gatagtccga
     8161 attccaagga ggattgtcga ccgcattcca gactccctgt aggttagctg gtagagcatc
     8221 tgggcagtct ctggacgaac taatcaataa ataccgtcca tactggaaga acagctcctc
     8281 caaagcctgc ccttcttgtg gcttataatt ttttaacaaa tcatctgtag tggatgcgtc
     8341 aacatcagct tccaaatcca attgaacacg ctggaataag gcttggtagt cctcgatatg
     8401 ccttgatttc aattgggtat agcccttttc tttagctgtg tccaccaagt ctatcacctg
     8461 ttgctctaaa tctagtttct tgcgataatt gctagcagga ttttgggcaa aatccgtctt
     8521 agcggccaag aagagattgg cataactggc tcctgatatc tgaaccctat ctgaccaaac
     8581 tctaatatct ccatccgttt cccaagctag ataactagca aaccgcagat cattatcctt
     8641 aactcttccc ttcatcaaga tatgagaatc agtaatatcc aacttacact ccttgtaatc
     8701 agatttttcc tgttcatact ttccatcaga agccaaatca caggtcaagg atagttctat
     8761 agtaaaatct agagtttcca acccttcctt agtaaaacat tgaaccaaga gatcatctgg
     8821 aaaactcgca aaagctttac gttcaaatcg cgttccctta tagacataag aagtcgtcgc
     8881 aagtgcctta ctaatattca gctgtctctg atagtccgtc acctgagaca aagtcgtacc
     8941 ttgctggctg aactcaatgt gaatatcccc aaaagacaga taggtcccat attgactcgt
     9001 ttttggccca attaggtgct gctcagccag ttccttaacc agattgtaat ctctcttctc
     9061 caaagcctgc cgaatctcag ctaaaaaaac atactgatcc tgaagatttc caccctgata
     9121 atctgaacta tcaggaagtg gacctccaga ccagagactt ttttcattaa attgaatccg
     9181 ttcagcccct ataaggccaa atacttttgc tcctaaagaa ccattgccta tcggtaaagc
     9241 ttcttcttcc caacccatgt aagtggttga agctggttgt ttgtaggcca gtacataatc
     9301 ttgtttttta ttccttatca tacttccata ctaacataaa aggctctata atatttgtag
     9361 tgggtaaccc cctatggata ttatggagcc tattttgtgt agaaaaaaag tcccatatga
     9421 cctataatga aaagcgacaa aacaactcat tagaaagaat cat
//
LOCUS       DAVKDZ010000073         9219 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000073 DAVKDZ010000000
VERSION     DAVKDZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9219
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.074"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            255..707
                     /locus_tag="VWZ30_001735"
     CDS             255..707
                     /locus_tag="VWZ30_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU9335420.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVYSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(742..2130)
                     /locus_tag="VWZ30_001736"
     CDS             complement(742..2130)
                     /locus_tag="VWZ30_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9335421.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            2196..3158
                     /locus_tag="VWZ30_001737"
     CDS             2196..3158
                     /locus_tag="VWZ30_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU9335422.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKAHLKLLIALNAGLTGQALKDYMEG"
     gene            complement(3499..4509)
                     /gene="ccpA"
                     /locus_tag="VWZ30_001738"
     CDS             complement(3499..4509)
                     /gene="ccpA"
                     /locus_tag="VWZ30_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEU9335423.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLVKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(4933..5532)
                     /locus_tag="VWZ30_001739"
     CDS             complement(4933..5532)
                     /locus_tag="VWZ30_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9335424.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLAFQADVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEIPVKTGLEVLEWIRAEKLETKVVVVTTFKRPGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(5533..6630)
                     /locus_tag="VWZ30_001740"
     CDS             complement(5533..6630)
                     /locus_tag="VWZ30_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9335425.1"
                     /translation="MLERLKSIHYMFWASLIFMLFPILPVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGLMLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVGGLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILAFVDLMTFGLVRIRIVEDLKEAQ
                     AKQNAQINLLLAENERSRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESTASMILLELVTNIIKHAKASKAYLKLERAEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVSPFSGEVSVISQKHPTEVQVRLPYKERK"
     gene            complement(6630..7367)
                     /locus_tag="VWZ30_001741"
     CDS             complement(6630..7367)
                     /locus_tag="VWZ30_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9335426.1"
                     /translation="MKRWIALNKIEFLLTKRQLVYYLLSVGMPTAFYLFFSGMYQDTP
                     GGPANFMRDYLISMTAFSMMSTAIFSFPAVLHTDKINNWQKTLRHSPINMVEYYLSKI
                     TSMLVDYLVSILVVFSVGHFVRGVEMPLGSWVGAAILLILGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNKGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(7369..8253)
                     /locus_tag="VWZ30_001742"
     CDS             complement(7369..8253)
                     /locus_tag="VWZ30_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9335427.1"
                     /translation="MNMIKVESLNKNIKGKAILKDISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRSYLPQENVIVQKLKVKELIAFFQKIY
                     PNHLSNQEIDQLLQFDQQQKEQFAEKLSGGQKRLFSFVMTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVQDLKAQGVTILYSSHYIEEVEHTADRILVLNKGGLIRDTTPLAIRSE
                     EIEKHFILPLAYKEVIEQSNLVENWSQKQDALQVVTREADAFWQLLVQAGCRIQEIEV
                     NNRSLLDTIFEETQKGDD"
     gene            complement(8240..8434)
                     /locus_tag="VWZ30_001743"
     CDS             complement(8240..8434)
                     /locus_tag="VWZ30_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335428.1"
                     /translation="MKKAFLYLFIGLSLVVWLVEMFTGWFDQAFLHQAIRGAWGLEFM
                     IFIAFPMGKEWLKGEYHEHD"
     gene            complement(8431..8637)
                     /locus_tag="VWZ30_001744"
     CDS             complement(8431..8637)
                     /locus_tag="VWZ30_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335429.1"
                     /translation="MEEEEMKNKMIVAVSLVTAGVMTYLMFSGLDEGFYHFPWELFAG
                     FGMMSWLIREGLKLVSDVKKEFEK"
BASE COUNT         2777 a         2096 c         1642 g         2704 t
ORIGIN      
        1 aattgacaga tcatgatcac ttctaccaag actgaaacta ggataattcc ataataaagg
       61 aaatttgatt tttgaaaaga gtttttcatc attctccctt ccttcacttc tttcctgcta
      121 tcctttcttt ttattttatc atatatttat atagagttta agccctaata ttcttttgaa
      181 aaatactttt tttgctttac ttctatagtg aaaaggttta caattatagt aagaaaattt
      241 caggaggttt cattatggca caacgttacc aaaatatcat ggtcgcaatc gatggttcta
      301 aggaagcgga cttggctttt gtcaagggag tttattctgc tctacgaaac gacgctaaac
      361 tcaccatcgc acatgtcatt gacacacgcg ctctccaaag cgtatccacc tttgatgctg
      421 aagtttacga agaactccaa gtcgacgctg aaagtctgat gaaagagtac gaaaaacgtg
      481 ctaaagatgc tggggtagca gatgttcata tcgtcattga aatgggaaat ccaaagaccc
      541 tgctagcacg tactattcca gatgccgagg aagtggacct catcctcgtt ggcgcaactg
      601 gtctcaacgc ctttgaacgc ctcttggtcg gctcttcatc tgaatacata ctccgccatg
      661 ctaaggtcga tttgctggtt gtgagagaac aagaaaaaac cttataatca caaagaaaag
      721 gagcccctag ctcctttttg tttacgattt atttctctct ttatggcgtt cgtaagcctt
      781 gagctggcgc tgcagttcct ttttaatagc aggttctgga gcatattttt cttcccaatt
      841 atctggtttt aagattttat gggtcactgg atcaaaatga gccttgccat ctggaaaaat
      901 tttccccata ttagcctgat ggacaatatc aaaaatacgt tctgggtcga cccccatcaa
      961 gacaaaactg ccgtaggtga agtaaagcgt gtcaatcaag gcatccactt gccctatcaa
     1021 atcttgctga gcaggtgtct tcttggctac tttacctgct gccttatcaa gggcctgatg
     1081 aagttgcgat acagcttgac caaaatcttc ttcagaagga ctggctgctc gaacaaactc
     1141 caccaattct tctattttaa aaccagccct atgggttgca ccctctaaat cccaagctcg
     1201 aggttcttct tgggttcgtt catccatcat gtggtggaag gtcttgacct tattgaaatg
     1261 atagtcacgg ctgacaaaga ctttttctga agacaaaaca ccaaaatgtt ccaaggcctt
     1321 gtggatccca tcttgttgat tgctagtggt aatgtgcttg gcagcttcct tgacactgct
     1381 actaccattt cccatagcga ccgacatacc gacgccagcc agcatttcaa ggtcattatc
     1441 tgagtcacca aaagccatga cttggttgag gtcaaagcca tattctttcc caactcggcg
     1501 aatgccttct aatttagaat ttccctgatt gatgacatcc gatgcaaaag gattgctacg
     1561 tgtcaatttc aagtcttcaa aatcagctgc cgccttctca gattcttctg gtgtcatcag
     1621 catcaaaact tggtagatag gctgattcat caggtgaagc aggtcctctt ccttttgggg
     1681 aacaaccttg ctgaccatgc gattaaaaga ctgactcacc gtccgagtta aaacagaggg
     1741 aacgaagcga ctaattcgtt gggaaaaaga acccagacca aaggacatga ttttagaacc
     1801 caacatggca tccttggtcc ctagggcaat ctccgtgccc tcttttttag catagctaat
     1861 tagatggcgc aaatgtaact tggaaatagg gctcgtgaac aagactctgt ctttactaaa
     1921 gatatactgg ccattatagg ttaccgcaaa atccagatcc aaatcgtcca tcaattcctt
     1981 aacaaaaaaa ggtcctcgcc ctgtcgctac gccaactagt accccttgtt ctttgacaat
     2041 cttaatcgcg tccttagtgg atttcaaaac actcttgcga ttgttgacca aggttccatc
     2101 gatatcaaaa aaaacagctt tgacttccat cctattccaa tctccccttt tgtgatacaa
     2161 tgattatacc acatttcaga aagagtgagt aaatcatgcc taagaaaatc cttgttttac
     2221 atacgggtgg aactatttcc atgcaggccg atgcttctgg cgctgttgtg acgagttcag
     2281 ataatcccat gaaccatgtg tccaacccac ttgaaggaat ccaagtccac gccttggact
     2341 tttttaacct tccaagtccc catatcaaac ccaaacatat gctggtcctc taccagaaaa
     2401 ttaaagagga agcagataac tacgatggag tggtgatcac acacggaacc gatactttag
     2461 aggaaacagc ctatttcctt gataccatgg aagttcccca tatgcctatc gttctaacag
     2521 gagccatgcg tagctccaat gagctcggta gtgatggtgt ttataattac ctaagtgctt
     2581 tacgagtggc cagcgatgac agggctgctg acaaaggagt tttggtcgtt atgaacgatg
     2641 aaatccacgc tgccaagtat gtcaccaaaa cacatacgac taatgtcagc accttccaga
     2701 ctccaacaca tggccccctc ggtctcatca tgaaacagga aatcctctac ttcaaaacag
     2761 ctgaacctcg tgttcgcttt gaccttgatc acatacaagg tttagtccct atcatctcgg
     2821 cttatgctgg tatgacagat gagctgattg atatgctgga tttagaacac ttggacggtt
     2881 tgattatcca agccttcgga gctggtaata ttcccaaaga aacggctcaa aaattagaaa
     2941 gccttctgca aaaaggaatc ccagtcgctc tggtatcacg atgctttaac ggtattgccg
     3001 agcctgttta tgcctaccag ggtgggggcg tacagttgca aaaagcaggc gttttctttg
     3061 ttaaagaact caatgcccaa aaagcccact tgaaactcct catcgccctc aatgccggac
     3121 taacaggaca ggctttgaaa gactatatgg aaggctaata ctcttcgaaa atctctgcaa
     3181 accacgtcac gtcgccttac cgtatgtatg gttactgact tcgtcagttc tatctacaac
     3241 ctcaaaaaca tgttttgagc tgacttcgtc agtttcatct acaacctcaa aaacatgttt
     3301 tgagctgact tcgtcagttc tatctgcaac ctcaaagcag tgcttttagc tgacttcgtc
     3361 agttctatct acaacctcaa aacagtgtgt tgcgcagcct ttaaccagcc gcctagtccg
     3421 ctctatggta ttcattaagt caacatctct tgtttaagag cacaaaatca ggaaatcttc
     3481 tcgattccct gattttttct atttacgttt tcgtgttgag ctacgttctg tcaaaccatg
     3541 aggtaagaga acttcacgtt cttccaactc ttccttatgc ataatcttgg tcaacatacg
     3601 catactaatg gcaccaaggt cataaagagg ttgggcaatc gttgtcaagt ttggacgggt
     3661 aaagcgtgag atttgtgaat catcactagt aataatttca aaatcttctg gcacagaaac
     3721 acccttatca gccaaaccgt tcaagactcc tgctgccaac tcatcacctg tcacaactgc
     3781 tgcagttgca tttgatgaaa tcaaacgctc tgctaaggcg taaccatcat catagctata
     3841 tttagattca aataccaaac cctcactata agtgattcct gcttttttca aggtttcctt
     3901 gtagccaact aaacgaacct taccattgat gtcatccact agcggaccgc taacgaaagc
     3961 aatacgctca ttttctttaa caaggtaact cactgcatca attgttgctt gcttatagtc
     4021 aatattgaca cttggcaact ggtgctccac atcgacagtt cctgcgagaa caatcggagt
     4081 acgtgaacgc gaaaattctg agcgaatttt atctgtcaag tgataaccca tatagataat
     4141 gccatctacc tgctttgaaa agagggtatt gacaacagaa acttctttct cgttatcttc
     4201 atcgctatta gctaggacaa tattgtactt gtacatttct gcaatatcat caatcccctt
     4261 agccaaactc gaaaaataac cattggtaat atttggaatc acgacaccga cagtggttgt
     4321 ctttttactt gcaagaccac gcgcaactgc atttggacga taatccaaac gatcaattac
     4381 ctctagcact tttttacggg tattctcttt tacattttta ttgccattga ccacacggct
     4441 gaccgtcgcc atggaaacac ctgcttcacg agcgacatca taaatggtta ctgtatcatc
     4501 tgcattcatt ccttttcctg tcctttctat ctcacacatt cttttacaag tagaggtgct
     4561 gattgaagct ctatatctac ttacaaaagt gaagatgtga aaatttcgtt ttcatatttc
     4621 tacttattcc attctatcac taattgtaaa cactttcaag tgttttttga agattgattg
     4681 aaaaaatttc atagaaaacc taggtttagc tccttgctac caccttagac taaacaaaaa
     4741 ggaggaaact aagccctcct aaagttatag taaaatgaaa taagaacagg ataaatcgat
     4801 caggacagtc aaatcgattt ctaacaatgt tttagaagta gaggtgtact attctagttt
     4861 caatctacta taggtattgt tccattcact accgtcaatt ttagcacata gtcttcataa
     4921 aatattatat catcataacc aaccagattc ttttgcgata ttagctgcct ctgttcgatt
     4981 accagcatcc agttttgaaa gaatattggt gacatagttt cgaatagttc cgtttgagag
     5041 gtaaagctga tctgcgattt cttggttgga taagccccgg gcgattccct ttaagacagc
     5101 aatctcttgt tctgtcaacg gattgggacg ggtcatcacc acttccatca attcaggcga
     5161 atactccttg cgtccttcga ggacggtgtg caaggtttgc atgaggtctg caatgcttcg
     5221 ttcctttaat acataagcat ctactccagc cttgaccgca cgttcaaaat accctgggcg
     5281 cttgaaggtc gtcactacaa ccacctttgt ttcgagcttt tctgctcgta tccactccaa
     5341 gacttcgagt cctgtcttaa caggaatttc tacgtcaagg atggcgatat ctacagactc
     5401 cttttctaat agttgaattg cttcttgacc attcttggct tgtaagacag actctacatc
     5461 tgcttgaaag gcaagcaact ggcacatggc atctcgcaac atactttgat cttctgcgac
     5521 taatactttc atctactttc tctccttata aggtagtcga acttgcactt ccgttggatg
     5581 tttctgactg attacactta cttctcctga aaatggagaa acacgatctc ggactgtatg
     5641 gagctcatct ccttttagaa aagcaaagcc gcagccatca tcacttactg ttaaaatgag
     5701 ttctttctct gcccgttcta atttcaggta ggccttcgac gctttggcat gtttgataat
     5761 attggtcacc aactcaagca aaatcatgga agccgttgac tccaattctt gagtaagact
     5821 agcagtatct agttggttat ccgtttccac ctcaattcca gcaatttcta acatcttttt
     5881 cacagtctct agttcggatg tcaaagttct agacttaaga ttttccacaa tggttcgcac
     5941 ttcattcatg gaatccttac tgatctggtg aatttctttt aattcctttt ccacctgtgg
     6001 ataagcctcc atctgaaata actgcaaggc taaatctgtc ttgacactca acatagcaaa
     6061 ggtatgtccc aagctatcat gcaaatcctg accgatacga ctgcgttcat tttcagcaag
     6121 caatagattt atctgggcat tttgcttagc ctgagcttct ttcaaatcct ccacaatacg
     6181 aatccgaacc aagccaaaag tcattaaatc gacaaaagca agaattacaa gtagatagaa
     6241 tagaaactca acttcgattc tctgaaaaat caacagttgg cccacaacaa ggacttgagc
     6301 aagaagaaaa gtccagacat gtaaagactt taaaccacct acgctgaaat gataacttaa
     6361 gagattggat aggaaaaaga aaaaccagat ataattaaca gcaacaaagg cagtattccc
     6421 aactacataa gtcagcatga ggccccaata tagccaagat aggcgctggc tcttagttgt
     6481 taaaacaccc aaatacgcca ctacaaatag aatatcaatc aataaatgcc aggcagaaag
     6541 ccacccagtc actacaggta ggatgggaaa aagcataaaa atcaaactgg cccaaaacat
     6601 atagtgtatg cttttcagtc tttcaagcat taagcattct ccttatgacc ttgaaggtaa
     6661 atagtcaaac caaataaaac tgctgaaaaa acaagtagat aaactgtggc tgatagattg
     6721 atgccaccct tgtttaagaa ggtcttgagt aactccatca actgataggt cgggagacac
     6781 ttaccgactg cttgcatcca gtctggaaat aaagagatag gcatccagag cccacctaaa
     6841 acagccaagc caaggtaaag aagattgccc acgacagaca tcagctgact agttggtaag
     6901 agcgtcaagg tcaagccaag cgctacaaaa gcaatacttc ccaaaataag caaaatcgca
     6961 gccccaaccc agcttcctag aggcatctct acacctctta caaaatgccc aactgagaaa
     7021 accaccaaga ttgaaaccaa ataatcaacc agcatacttg ttatctttga tagataatat
     7081 tctaccatat ttataggact atgacgtaat gttttctgcc agttgttgat tttatctgta
     7141 tgtaaaacag ctgggaatga aaagatagct gtcgacatca tggaaaaagc cgtcatggag
     7201 atgaggtaat cacgcataaa attagctggt ccaccaggtg tgtcctggta catgcctgaa
     7261 aagaataaat agaaggctgt cggcatccct acggataata gataatagac taattgtcgt
     7321 ttggtcaata gaaattctat cttgtttagt gcgatccatc gtttcatctt agtcatctcc
     7381 cttttgtgtt tcttcaaaga ttgtatccaa caagctacga ttattgactt caatttcttg
     7441 aatcctacat cctgcttgca ctaacagttg ccaaaaagca tccgcttcac gtgtaactac
     7501 ttgcaaagca tcttgttttt gtgaccagtt ttcaaccaag ttagactgct caatgacttc
     7561 cttgtaagct agaggaagga taaagtgctt ttcaatctcc tcactgcgta tggctagagg
     7621 tgttgtatcg cgaatcaacc ctcccttatt caagaccaag attcggtccg ctgtatgctc
     7681 tacctcttcg atgtaatggg acgaatagag aatggtaact ccctgcgctt ttagatcctg
     7741 aacaatctcc caaaaacgtt ggcgggttga ggtatccatg gccgcagttg gctcatctaa
     7801 aaagacaagt tttggtcggc caatcaaggt catgacaaaa gagaaaagac gcttttgccc
     7861 acctgacaat ttttctgcaa attgctcttt ttgttgctgg tcaaactgca atagttgatc
     7921 gatttcctga ttactcaagt gatttggata aattttttga aagaaagcaa tcaactcttt
     7981 tacctttagt ttctgaacga ttacattctc ttgagggaga taggatctag tgtagtctaa
     8041 cttagagctc gtcactggca agccttggat ggatacttga ccgcttgtga ccagtttatc
     8101 tccaagcaga cagtccaaga gtgtggtctt cccagcacca ttgggcccaa tcaaggcgac
     8161 gcattcacct tcagctacct caaaggaaat atccttcaaa atagccttgc ccttgatgtt
     8221 tttatttagg ctttctacct taatcatgtt catgatattc tcctttcagc cactcctttc
     8281 ccataggaaa ggcgataaaa atcataaatt ctagtcccca ggcaccgcga atggcttggt
     8341 gaaggaaggc ttgatcaaac caacctgtaa acatttctac caaccatact actagtgata
     8401 gtccaataaa aagatagaga aacgcttttt tcatttttca aactcctttt tcacatctga
     8461 gactaatttc aaaccttctc ggataagcca ggacatcatt ccaaatcctg caaatagctc
     8521 ccaaggaaaa tgataaaatc cttcatccaa tcctgaaaac atgagataag tcatgactcc
     8581 tgcggttact agactcactg cgacaatcat tttatttttc atctcttctt cctccatttc
     8641 atacttcaat tatagacttt tgaagtttgc ggagctagat gcttctgtca ctaggaaata
     8701 tgacaactgt cataaaagat tctgttcaaa acaagcaaga tacactatac aataaaacac
     8761 aatcagaaaa atctaaggca acttcctcaa aagagatatc aaacccaatt cacaccataa
     8821 tgtaaactaa tacttattta aaatcaaaaa gagtagaaat ttttatcaga caaacacata
     8881 tatagtgtat tgaatctata acagtaggcc ttaaatacta aaatatttct ataaattaat
     8941 ttaactttcc tgatagagct gttcatatct tatttcaatt ctctaaatta tacgttgaac
     9001 aaaacccttc tatttctttc ttaaaaattt ataagagtta taaaatctgt taaatttcaa
     9061 tgtgtatacc taaactacgg tatttattga aaaaactgga gacaaaaagt atacgttgcc
     9121 aaaatgaatt actgaaaatc aaaaaagaga gaaccaaact gattccctct taatgtatat
     9181 aatatctagt tttaaaaata cacactcaca tatctctgt
//
LOCUS       DAVKDZ010000074         9000 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000074 DAVKDZ010000000
VERSION     DAVKDZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9000
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.075"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            1..594
                     /gene="ruvA"
                     /locus_tag="VWZ30_001745"
     CDS             1..594
                     /gene="ruvA"
                     /locus_tag="VWZ30_001745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU9335430.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            604..1167
                     /locus_tag="VWZ30_001746"
     CDS             604..1167
                     /locus_tag="VWZ30_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU9335431.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            1164..1841
                     /locus_tag="VWZ30_001747"
     CDS             1164..1841
                     /locus_tag="VWZ30_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9335432.1"
                     /translation="MMSNKNKGILIFAILYTVLFMFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(1978..3009)
                     /locus_tag="VWZ30_001748"
     CDS             complement(1978..3009)
                     /locus_tag="VWZ30_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU9335433.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLRLNPIFLPHS
                     LKGLDFIKDHPEARAENLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(3264..3947)
                     /locus_tag="VWZ30_001749"
     CDS             complement(3264..3947)
                     /locus_tag="VWZ30_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEU9335434.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(3959..4903)
                     /locus_tag="VWZ30_001750"
     CDS             complement(3959..4903)
                     /locus_tag="VWZ30_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9335435.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            5035..7866
                     /gene="uvrA"
                     /locus_tag="VWZ30_001751"
     CDS             5035..7866
                     /gene="uvrA"
                     /locus_tag="VWZ30_001751"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU9335436.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMTAFGVDMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVVTNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGDQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINNILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            7859..8920
                     /locus_tag="VWZ30_001752"
     CDS             7859..8920
                     /locus_tag="VWZ30_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9335437.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIVGIVKDMGLTRIGFEDEISVSYY
                     HRMQATFAGLDLLPQTQFVEGLRMIKDEAEIAAIRKACFISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMTLTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
BASE COUNT         2516 a         1808 c         2102 g         2574 t
ORIGIN      
        1 atgtacgcat atttaaaagg aatcattacc aaaattactg ccaaatacat tgttcttgaa
       61 accaatggta ttggttatat cctgcatgtg gccaatcctt atgcctattc aggtcaggtt
      121 aatcaggagg ctcagattta tgtgcatcag gttgtgcgtg aggacgccca tttgctttat
      181 ggatttcgct cagaggatga gaaaaagctc tttcttagtc tgatttcggt ctctgggatt
      241 ggtcctgtat cagctcttgc tattatcgct gctgatgaca atgctggctt ggttcaagcc
      301 attgaaacca agaacatcac ctacttgacc aagttcccta aaattggcaa gaaaacagcc
      361 cagcagatgg tgctggactt ggaaggcaag gtagtagttg caggagatgg ccttcctgcc
      421 aaggtcgcag tgcaagcaag tgctgaaaac caagaattgg aagaagctat ggaagccatg
      481 ttggctctgg gctacaaggc aacagagctc aagaaaatca agaaattctt tgaaggaacg
      541 acagatacag ctgagaacta tatcaagtcg gcccttaaaa tgttggtcaa ataggagcag
      601 agaatgacaa aacgttgttc gtgggtcaag atgaccaacc cactctacat cgcctatcat
      661 gatgaggagt ggggccagcc cctccatgat gaccaagcgt tgtttgagtt gttgtgtatg
      721 gaaacctatc aggcaggcct gtcttgggaa acggtactca acaaacgcca agctttccga
      781 gaagcctttc atagctatca aattcactca gtcgcagaga tgactgacac tgaattggaa
      841 gccatgctgg agaatccagc tatcattcga aatagagcca agatttttgc tacacgcgct
      901 aacgcccaag cctttctaca gttacaggca gagtacggct cttttgatgc ctatctttgg
      961 tcttttgttg aggggaaaac tgtcgttaac gatgttcctg attatcgcca agccccagct
     1021 aaaacaccct tatctgagaa attagccaaa gatctcaaaa aacgaggctt caagttcaca
     1081 ggcccagtcg ccgtattgtc ttttctacag gctgcagggc tagttgatga tcacgagaat
     1141 gattgtgagt ggaaaggtct taaatgatgt ctaacaaaaa taagggaatt ctgatttttg
     1201 cgattctcta tacagtcctc tttatgtttg atggcgttaa attgctggct tctttaatgc
     1261 catctgccat tgcaaattat cttgtttatg tagttttagc tctatatggc tccttcttgt
     1321 tcaaggatag attgatccaa caatggaagg agattagaaa gactaaaaga aaattcttct
     1381 ttggcgtctt aacaggatgg ctctttctca ttctgatgac tgttgtcttt gaatttgtat
     1441 cagagatgtt gaagcagttt gtgggactag atggacaagg tctaaatcag tctaatattc
     1501 aaagtacctt tcaagaacaa ccactactga tagctgtttt tgcttgtgtc attggacctc
     1561 tggtagaaga attatttttc cgtcaggtct tattgcatta cttgcaggaa cggttgccag
     1621 gtttactaag cattattctg gtaggacttg tttttgctct gactcatatg cacagtttgg
     1681 ctctatcaga gtggattggt gcagttggtt acttaggtgg aggccttgcc ttttctatta
     1741 tttatgtgaa agaaaaagag aatatctact atcccctact tgttcacatg ttaagcaaca
     1801 gcctctcctt aatcatttta gctatcagta tagtaaaatg aaatgagaac aggacaaatc
     1861 gatttctaac aatgttttag aagtagaggt gtactattct agtttcaata tactgtaata
     1921 tgtgatgaaa atgccagtaa tgataccgag aaaaaagctg agaaactttt cccagcttta
     1981 tttgttatag tcaaagagaa tgacttgttc ctgtgcatct acatgagcat ggaccccaaa
     2041 gggtacaatt gctcttggag ttgcgtggcc gacattcaga ttatagacaa tcgggatatt
     2101 gctgtcaatg atatccaata gtgcctcttt atagtcgtca tggaaagttt catccatagg
     2161 ttttccgacc aagagtccat tgatgaccgc gaatatgcca gtgtccttta aagttagcaa
     2221 catctttttg aagtcttctg gcttaggctt ttcttcgctt gtttcgagca agaggatttt
     2281 cccttcccag tctgacaagt cagggaaaag tttgtatttt tggcagagct ccgtgctatc
     2341 tgcgtatcga gagttgtcaa agatatcgta gagggattcg aggcaaccac cgaggatttt
     2401 cccctcgaac tgggcacttc cttgcaacaa gtcaaaacct gtatttgtat gactgacacg
     2461 aggtgttccc agggccgtgg gactaaaatc agttcgttcc tcataccaaa cgtcactagg
     2521 gcggatttct gaaattcttc ccgtctcaat caattcttta aagtagtgaa ggctataggc
     2581 tagcatttct ttgtctaatt cacaaatgtc tgctaaaaag gattgaccat aaaaagtctt
     2641 gattcctaat ttatgcaaca tgaggtggtt catggttgta tccgagaagc caagaaaaat
     2701 tttttgtttg ataacctttt ggagttggtc attttcaaaa agataaggta gcaagcgata
     2761 ggtatcgtct ccaccgatgg cacataggat catgtcgatg ctatcatcag aaaaggcatg
     2821 aatcaaattc tctgcacgag cttcaggatg gtccttgata aagtctaatc cttttaacga
     2881 atggggcaaa aagatgggat tgagtctcag atccttgaga cgttggatac ccaagtccac
     2941 ttcgtgtttg acaaaatcct ctccgataat gccactagat aaactaacaa taccaatagt
     3001 agaaaccata tctcatcctc ctagaaatag attgagccta ttttatcaca aaatataaga
     3061 gaaagctatg tgggatttca gaagaatact tttaatactc ttcgaaaatc tcttcaaacc
     3121 acgtcagttt tatttgcaac ctcaaagctg tgctttgagc aacctgcagc tagcttccta
     3181 gtttgctctt tgattttcat tgagtataac tcaaaaaagt aagtgaaaaa gcaaccgcac
     3241 ctgcagttgc ttttatcgtt ttcttattct tgatagcgtg ttggtcctgc acttgcacta
     3301 cggatattca agcgtttctt gagatagaag cgaagggcta ggagggctgc tccaataata
     3361 gctagtggca atggatccag tactgggtta aggctagctg gtaggaagct tgttgcaaag
     3421 aagacaagca accaaaggaa catagaagct aggataacta gtacagattt ccagaaaggt
     3481 ggacgttgac tgcgatccat atctggtcca tagtattggt aaacaaagta gtacatcaag
     3541 tagaaggcaa atccaccaac cagtccaact aatagaagag taatcaatcc atagccgaaa
     3601 gcttgatctg ccgcaaagaa ggttgtgagg gcgctgacaa gggcaaagag gctagtgatg
     3661 aaaagagctg agtccataat catcagtttt ggatcatcat tttcttttgg atgctctttt
     3721 tcgtactgct ctttgacagt gaagctatga gcccaatgag ttggtgcgcc gtatagggaa
     3781 cgggcagttg tacctttaga ttgctcctca aggatttggg gaatgacttc ctcaaaaata
     3841 gtctggattt cagcgtctgt tttcccatct ttgatgaatt gttgggtagc aatgtggaca
     3901 aactcttggt ttttcttagt taatttttgt agatcaattt gagacatagg aactcctctt
     3961 agaaccattt tttatggatg agatagagag tgagcgagac actcatagca aaggcgataa
     4021 agacgattaa ccagaaggca tttggctctc cgtttagggg gatttcatta tccttaaagt
     4081 tcatcccgta ggcagaaaag accatggttg ggatggacat gacgatggtc acaagggcca
     4141 aggttttcat gatgttgttc tggttgttag aaatgataga ggcaaaggtc tctgtcatag
     4201 aatgcaagac gtttccataa atatctgcca tctcgatggc ctgttgggtt tcaatcaggg
     4261 tgtcttcaag caggtcttcg tcctcaaggt atttcttgat attgctggtt gaactggtca
     4321 atttcttaat cacgcgctca tttgttttga gggaggcctt gaaatagacg atagtttttt
     4381 ccaattccat gagctcaatc aattcttcat tacgagttga ttgatgcagt tgactttcga
     4441 tttgttcact cttgcggtcg attgaacgaa gggctgttag gtaaagctct gcattgcgat
     4501 aaagaatttg aaagataaaa cgtgaacgca tgaaggtata gaaattacgc aatcgacggt
     4561 tgataaagac atcaaggaca ggtagtggtt ccaaacacgt agtgataatg gtttcctcag
     4621 tgatgataat accaagcggg atggttacgt agtaggtgcg gttatttctt tcctccgtga
     4681 ccggcacgtc tacgataatc agggtatact cgtcttcaat ggtaatacga gacatttctt
     4741 ccgcatcgag cggtgctcga aggtcggcaa tatcaatatc gaaggtgttg gcgatttcga
     4801 gtgattcatt ttgagtcgga ttgacgagat tgatccaagt acccggttca agcgtatcga
     4861 tctctttaaa ttcagttgtt gtagagagaa aaacttgttt catatccctt atcctttctc
     4921 atttttcaga ttttttcaca ctgtactatt atactacaaa atcggccttt tgggtaatag
     4981 aatctcactt tttttggtat aatggtaagc aataatggac tagaaagaac aaagatgcaa
     5041 gataaaattg tcattcatgg ggcgcgtgcc cataatttaa aaaatattga tgtggagatt
     5101 ccgcgagaca agttggttgt cgtgactggc ttgtcaggtt cagggaagtc cagtctggct
     5161 tttgataccc tctatgcgga gggacaacgt cgctatgtag agagtttgtc cgcctacgct
     5221 cgtcagttct tgggaaatat ggaaaaacca gatgtagatg ccattgatgg tctcagccca
     5281 gctatttcca tcgaccagaa aacgactagt aaaaatcctc gctcgacggt gggaacgacg
     5341 actgaaatca atgactatct gcgtctcctc tacgcacgtg tggggacgcc ttactgtatc
     5401 aacggacatg gagctatcaa tgcttcttct gtggagcaaa tcgttgataa ggttttggag
     5461 ttacctgaac gccagcgctt gcagatcttg gctcctgtca tccgcaagaa aaaaggccaa
     5521 cataagagtg ttatcgagaa ggttcagaaa gacgggtatg ttcgtgtccg tgtggatggg
     5581 gaagtctatg atgtgaccga agtgccagag ttgtctaaga gcaagcaaca caatatcgat
     5641 gtcgtggttg accgtattgt tatcaaggag ggcattcgta gccgtctctt tgattccatt
     5701 gaagctgccc ttcgtatcgc agaaggttat gtgattatcg acaccatgga cgattcggag
     5761 ttgctcttct ctgagcatta tgcctgtcca gtttgtggat ttactgtccc agagttagag
     5821 cctcgtctct tctctttcaa tgctcccttt ggctcttgta gtgagtgtga cggcttgggg
     5881 attaagctgg aggtggatac tgatttggta gtgccagatg ccagcaaaac gctacgtgag
     5941 ggggctttgg caccttggaa tcctatctca tctaactact atccaaatat gctagagcag
     6001 gctatgacag cctttggagt ggatatggat aagccctttg aggacttgtc agaagaagat
     6061 aagaacttga ttctctacgg ctcggatggc aaagaatttc attttcacta tgagaatgaa
     6121 tttggcggtg tgcgcgatat tgacattcct tttgagggag tcgtcactaa tatcaagcgt
     6181 cgttaccatg agactaacag tgattacacg cgcactcaga tgcgtctcta catgaatgag
     6241 ctgacctgcg gaacctgtca aggctatcgt ctcaatgacc aggccttgtc tgtccgtgtg
     6301 ggcgaccagc aagggccaca tattggagaa atctcagacc tgtctatcgc tgaccacttg
     6361 gacttggtga gccagttaac tttgtctgaa aatgaagcca tcattgctcg tcccattctc
     6421 aaggaaatca aggatcgttt gaccttcctt aataatgtgg gtcttaacta tctaaccctg
     6481 tcacgttcag caggaaccct ttcaggtggg gaaagtcagc gcattcgttt ggcaacccag
     6541 attggttcca acctatcagg cgtcctctat atcctagacg agccgtcaat cggtcttcac
     6601 cagagggaca atgatcgcct gattgccagt ctgaaaaaga tgcgtgactt gggcaatact
     6661 cttatcgtgg tggaacacga cgaagatacc atgcgtgagg ctgattatct gattgacgtt
     6721 ggtcctggtg ccggtgtttt tggtggtgag attgttgcag caggtacacc taagcaagtg
     6781 gctcgcaaca gcaagtctat cacaggtcag tacttgtcag gcaaacgtgt catcccagta
     6841 ccagaagagc gccgtgtcgg aaatggtcgt tttattgaag tgataggagc gcgtgagaac
     6901 aacttgcaaa atgtcacggc tcgctttcca ctaggaaaat ttatcgcagt gacaggtgtg
     6961 tcgggttcag ggaaatcgac tctaatcaac aacattctca aaaaagccat tgcccagaag
     7021 ctcaaccgta attcagacaa acctggtaag tttaagacta ttacagggat tgagcatgtc
     7081 gaccgtttga ttgatattga ccagagccct atcggacgga cgccgaggtc taacccagct
     7141 acctatacag gagtttttga cgatatacgt gacctttttg ctcagacaaa tgaagccaag
     7201 attcgaggct ataagaaggg gcgcttcagt ttcaacgtca agggaggtcg ctgtgaagct
     7261 tgctcaggtg acgggattat caagattgag atgcacttct tgccagatgt ttatgtggct
     7321 tgtgaagttt gccacggaac ccgctataac agtgaaaccc tagaagttca ctacaaggaa
     7381 aagaatattt cgcaggtctt ggatatgacg gtcaacgatg cggtagaatt tttccaacac
     7441 attccgaaaa ttcaacgcaa actccagacc atcaaggatg tagggctagg ctatgtgacc
     7501 ttgggacagc cagctaccac cctttctggg ggagaagccc aacgtatgaa gttagctagc
     7561 gaactccaca aacgctcgac aggaaaatct ttctacattc tggatgagcc gacgacaggg
     7621 cttcatacag aggacattgc tcgcctgctc aaggttttag ctcgctttgt agacgatggc
     7681 aatacagtcc tcgtcatcga gcacaatctg gatgttatca agacggcaga ccatatcatt
     7741 gacttgggac ctgagggcgg tgtcggtggt ggaaccatca tcgtaacagg aactccagaa
     7801 gaagtagctg ccaacgaagc cagctataca ggacactatt tgaaaggaaa gttacatcat
     7861 gaataaacgc gtacaagcat ttctagctaa aatgcaagaa aaagaactag atggtatcat
     7921 catcaacaat cttaaaaacg tctattattt gactggtttt tggggctcaa acggaacagt
     7981 ctttatcagc cgtgaccgtc aggtcttagt gacagactct cgctatatca tcgcagctaa
     8041 gcaagaaacc agtggttttg agattgtggc tgatcgtgat gaattggctg tcattgtagg
     8101 aattgttaag gacatgggct tgactcgtat cggttttgaa gatgagattt cagtgtctta
     8161 ttaccaccgt atgcaggcaa cttttgcagg tttggacttg cttccacaaa ctcagtttgt
     8221 ggaaggtctt cgtatgatta aggatgaggc agagattgca gcgattcgca aggcttgttt
     8281 tatctcagac caagctttcc gcgatgcgct tgactttatc aaaccaggaa aaactgaaat
     8341 tgagattgcc aacttccttg atttccgcat gcgtgagttg ggagcatctg gcttatcttt
     8401 tgatacgatc ctagctagcg gtatcaattc ttctaaaccc catgcccatc caatgcacaa
     8461 accagtggag ttgggagaag ccattaccat ggacttcggc tgtctctatg accactatgt
     8521 cagtgatatg acccggacta tctatctagg gcatgttagc gatgagcagg cagagattta
     8581 caatacggtt ctaaaagcta accaagcctt gattgaccaa gctaaggcag gcttaggttt
     8641 ccgtgacttt gacaaaatcc ctcgtgatat tatcattgag gcaggttatg gcgactactt
     8701 tactcacggc attggccacg gtattggtct ggatatccat gaggaaccct actttagcca
     8761 gacttctaca gaaactatta agacaggtat gaccttgacc gatgaaccag gtatctatat
     8821 cgaaggcaaa tatggcgttc gtatcgagga tgatatcctg attacagaga caggttgtga
     8881 attattgacc ctagctccaa aagagttgat agtcatttag ttgtttgtca agaaaaaagt
     8941 acgaaaatga cgaaaaaagt caaaaaaaat taaaaatagg tcgcaagtcg gatgtttttt
//
LOCUS       DAVKDZ010000075         8988 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000075 DAVKDZ010000000
VERSION     DAVKDZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8988
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.076"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(637..1665)
                     /gene="queA"
                     /locus_tag="VWZ30_001753"
     CDS             complement(637..1665)
                     /gene="queA"
                     /locus_tag="VWZ30_001753"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU9335438.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVSTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVNN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPRYGWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            1816..2523
                     /locus_tag="VWZ30_001754"
     CDS             1816..2523
                     /locus_tag="VWZ30_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU9335439.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            2663..2839
                     /locus_tag="VWZ30_001755"
     CDS             2663..2839
                     /locus_tag="VWZ30_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU9335440.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            2969..3904
                     /locus_tag="VWZ30_001756"
     CDS             2969..3904
                     /locus_tag="VWZ30_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEU9335441.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIVILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            3897..4685
                     /locus_tag="VWZ30_001757"
     CDS             3897..4685
                     /locus_tag="VWZ30_001757"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU9335442.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            4686..5069
                     /locus_tag="VWZ30_001758"
     CDS             4686..5069
                     /locus_tag="VWZ30_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU9335443.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            5085..5474
                     /locus_tag="VWZ30_001759"
     CDS             5085..5474
                     /locus_tag="VWZ30_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU9335444.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            5558..6688
                     /locus_tag="VWZ30_001760"
     CDS             5558..6688
                     /locus_tag="VWZ30_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU9335445.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            6693..7430
                     /locus_tag="VWZ30_001761"
     CDS             6693..7430
                     /locus_tag="VWZ30_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEU9335446.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSNFDKKLIRGPKYESLEELISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            7441..8214
                     /locus_tag="VWZ30_001762"
     CDS             7441..8214
                     /locus_tag="VWZ30_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEU9335447.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVNVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVADLPIAPTHVVSSLAEWDFDEN"
     gene            8204..8821
                     /locus_tag="VWZ30_001763"
     CDS             8204..8821
                     /locus_tag="VWZ30_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEU9335448.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLPIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
BASE COUNT         2592 a         1663 c         2042 g         2691 t
ORIGIN      
        1 tttttttcta caataaaata ggctccataa tatctatagt ggatttaccc actacaaata
       61 ttatagagcc aaaaagtgaa caagcctagt cttctgtaca ttagaattca gatttgttca
      121 ctttttgttt aatactcaat gaaaatcaaa gagcaaacta gaaaactagt cgcaggttgc
      181 tcaaagtact gctttgaggt tgtagataga actgacgaag tcggtcacat atataattca
      241 aggtgacgtt gacgcggttt gaagagattt ttgaagagta taagtctttg attttgagac
      301 taaggagagt cagggtcaaa gtagtgaatg attctgaaat aactcataac agtctcttga
      361 gaggacagat aagagtaaca aatatggtgc tactcatgtg tcttgcattt taatttacaa
      421 acagcgaaga aagtatagga agctgaaata aaatatagat gaatcaattt ggagagtcaa
      481 cgcaatctat ggcaatattt tcgaagctag ggggaagcat tttaatttca atgtactagg
      541 cgtcttctat agctagctag agtttcaatt gtagatgttc tttgaaataa tatcttagcg
      601 atttattggt attagaagat ttagagaaat tctttctcaa taaataaaca tggcgtcacc
      661 aaaactgaag aagcggtagt gttcttggat ggcatggtgg taggcatcta agactaattc
      721 acggcctgca aaggcagaaa ccaacatgac cagagttgat tttggcaggt ggaagttggt
      781 tgagaaggca tccacgacct tccacccata cctaggcttg ataaagatat tggtccaacc
      841 agaatctgct tggatttgcc catcaaactt ggaaccaata gtttccaagg tgcggataga
      901 agtggttcca acagcgatga cacgaccacc attttctttg acagagcgaa gggtggcagc
      961 agcttcttca gaaagttggt agaattctga gtgcatttcg tgttcgtcca gattattcac
     1021 agaaacaggt ctaaaggttc cgagtccgac atggagagtc agatagacta gatgaacacc
     1081 cttagcttgg atttctgcca gcagttcttt ggtgaagtga agaccagcag tcggtgctgc
     1141 agcagagccg ctttccttgg cgtagacggt ttgataacgt tcacggtcat ctaatttttc
     1201 gtggatataa ggtggcagag gcatttctcc cagactttcc aagacttcta ggaaaattcc
     1261 ttggtattca aagcggacaa tgcgtccccc gtgggtcaat tcttctgtaa cgacagcgct
     1321 gaggcggcca tcaccaaagc tgatacgagt actgaccttg aggcgtttgg caggtttagc
     1381 cagaacttcc cactcgtctc cactagtgtt cttaaggagg agaagttcca catgacctcc
     1441 tgtctccact ttttgaccat agaggcgggc agggagaact cgggtgtcgt tcatgacaag
     1501 ggcatcacca ggttccagca tatcaataat agagtggaag tgtttatctt gcatttctcc
     1561 tgtctcacgg ttgacgatga ggagtttgga ggcatcacgt ttttcaaggg gcgtttgggc
     1621 aatcaattcc tctggcaagt ggaaatcaaa atcagctgta ttcatatatc tgttcattct
     1681 ttctaagttc taatactatc cattataaca cgtttcaagt ttggacgctc tttttttaga
     1741 aattaaatcg ttttcacttg acaaaaattg gtctatacca tataataaat atagacagaa
     1801 atggaggatg aaaagatgaa agttattaaa gttgaaaacc aagttcaagg tggaaaagtt
     1861 gcttttgaga ttttgaagga aaaattggct aatggagctc aaactctagg acttgcgaca
     1921 ggaagtagcc cacttgaatt ttacaaggaa attgttgaga gtgaccttga tttttcaaat
     1981 ctaaccagtg tcaaccttga tgagtatgtg ggccttgatg gggacaatcc acagtcttat
     2041 cgttacttca tgcaagaaaa cttgttcaac caaaaaccat ttaaagaaag tttcttgcct
     2101 cgtggtgtta aggacaatgc tgaagctgaa gttgaacgct acaaccaaat tttggctgac
     2161 catccagttg acctccaaat cttgggaatc ggtcgcaatg ggcatatcgg ctttaatgag
     2221 cctggtactc catttgacag tcaaacgcat ctagtagaac ttgaccagtc tactatcgaa
     2281 gccaatgcac gcttctttgc taagattgaa gacgtcccaa cccaagctat ttcaatgggg
     2341 attaaaaaca ttttggatgc caagtctatt attctttttg cttacggtga gtcgaaagca
     2401 gaagccattg ccggaacagt atctggccca gtgactgaga acctacctgc aagtagcctc
     2461 caaaatcacc ctgatgtaac catcatcgca gatgctgaag cgcttagctt actcgaaaaa
     2521 taaaaaagca atgttctatt gaacgctttc aactcttgta taaatggaag aaaaaatcaa
     2581 aattaaaccg catttttgct tgacaattat tccttttacg tgtagaatga aatagatatt
     2641 gaacttgaag ggagtgaaaa aaatgtctaa aacagtagta cgtaagaatg aatctcttga
     2701 cgacgcactt cgccgtttca aacgtgcggt tactaaagct ggtactcttc aagaaacacg
     2761 caaacgtgaa ttctatgaaa aaccttctgt aaaacgtaaa cgtaaatcag aagtagctcg
     2821 taaacgtaaa aaattctaat tgaaatgaaa ggctagagaa atctagtcct ttttctttta
     2881 aataaatact ccaaagcctg caaaaatctg aaacttcctc ctacaatttg atataataga
     2941 gagaagaact catttgaagg aggaaatgat gtcggtttta gtaaaagaag tgattgaaaa
     3001 gcttagacta gacattgtct atggcgaacc agaattgctt gaaaaggaaa tcaatacagc
     3061 ggatattacg cgacctggtc ttgaaatgac aggctatttt gactactata caccagagcg
     3121 gattcaactt ttggggatga aggagtggtc ttatctgatc agcatgcctt ccaacagccg
     3181 ttatgaagtt ttgaaaaaaa tgtttctacc tgagacacca gcagtcattg ttgcccgtgg
     3241 tttggtggtt ccagaggaga tgttaaaggc tgctagagaa tgtaagattg ttattttaac
     3301 cagccgtgca gctaccagtc gtttatctgg agagttatct agctatctgg attctcgttt
     3361 ggcagaacgt accagtgtgc acggtgtctt gatggatatt tatgggatgg gcgtcttgat
     3421 tcagggagat agtggaattg gtaagagcga gacaggtctt gagcttgtca aacgtggtca
     3481 ccgtttggta gccgatgacc gtgtcgatat ctttgccaag gatgagatta ctctctgggg
     3541 tgaaccagct gaaattttga aacacttgat tgaaattcgt ggggttggta ttatcgatgt
     3601 tatgagtctc tacggtgcga gtgctgtcaa ggattcttca caggttcagc ttgctgtcta
     3661 tttggaaaat tacgatacgc ataagacctt tgatcgtctt ggaaacaatg cagaggaact
     3721 tgaagtttct ggcgtagcca ttcctcgtat tcgtattcca gttaaaacag gtcgtaatat
     3781 ctctgttgtg attgaggcag ctgccatgaa ttatcgtgcc aaggaaatgg gctttgatgc
     3841 gacccgtttg ttcgacgaac gactgacaag tctcatcgct cgaaatgagg tgcaaaatgc
     3901 ttgatccaat tgctattcaa ctaggacccc tagctattcg ttggtatgcc ttatgtattg
     3961 tgacaggctt gattcttgcg gtttatttga ccatgaaaga agcacctaga aagaagatca
     4021 taccagacga tattttagat tttatcttag tagcctttcc cttggctatt ttaggagctc
     4081 gtctctacta tgttattttc cgatttgatt actatagtca gaatttagga gagatttttg
     4141 ccatttggaa tggtggtttg gccatttacg gtggtttgat aactggggct cttgtgctct
     4201 atatctttgc tgaccgtaaa ctcatcaata cttgggattt tctagatatt gcggcgccta
     4261 gcgttatgat tgctcaaagt ttggggcgtt ggggtaattt ctttaaccaa gaagcttatg
     4321 gtgcaacagt ggataatctg gattatctac ctggctttat ccgtgaccag atgtatattg
     4381 aggggagcta ccgtcaaccg actttccttt atgagtctct atggaatctg cttggctttg
     4441 ccttgattct gatttttaga cggaaatgga agagtctcag acgaggtcat atcacggcct
     4501 tttacttgat ttggtatggt ttcggtcgta tggtcatcga aggtatgcga acagatagtc
     4561 tcatgttctt cggccttcga gtgtcccaat ggctgtcagt tgtccttatc ggtctcggta
     4621 taatgatcgt tatttatcaa aatcgaaaga aggcccctta ctatattaca gaggaggaaa
     4681 actaaatgtt agaagttgca tatattcttg ttgccctagc tttgattgtc tttttggtct
     4741 atctgatcat tactgtacaa aagcttggtc gtgtcatcga tgaaacagaa aagacgatta
     4801 aaaccttgac ttcagatgtg gatgtgacct tgcatcacac caatgagttg ttggctaagg
     4861 tcaatgtctt ggcagatgat atcaatgtca aggtggctac gattgatcca ctcttcagtg
     4921 ctgttgcaga tttatctcta tctgtttcag acctcaatga ccatgcgcgt gtcttgagca
     4981 agaaagcttc atcagctggt tcaaaaacac tcaagactgg tgcaagtctg tcagctcttc
     5041 gtcttgcaag taaatttttc aaaaaataaa aaaggagaat ccttatgggt aaattatcct
     5101 caatcctttt aggaacggtt tcaggtgcag ctcttgcctt gtttttaaca agtgataagg
     5161 gcaaacaagt ttgcagtcag gctcaagatt ttctagatga tttgagagaa gatccggagt
     5221 atgccaagga gcaagtctgt gaaaaactga cagaagttaa ggagcaggct acagattttg
     5281 ttctgaaaac aaaagaacag gttgagtcag gtgaaatcac tgtggacagt atacttgctc
     5341 aagctaaatc ctatgctttt caagcgacag aagcatcaaa aaatcaatta aataatctca
     5401 aggagcaatg gcaagaaaaa gccgaagctc ttgatgactc agaagagatt gtgattgata
     5461 taacagaaga ataaaccatc accatctccg gacggactat gtatctgggg atggtgattt
     5521 ttatctggaa tctagtcttt gtggtataat aattactatg cagaaaaaac caacgtcagc
     5581 ctatgtgcac atcccatttt gtacccagat ttgttattat tgtgattttt caaaggtctt
     5641 catcaaaaat cagccagtcg acagctattt agagcatctg ctggaagagt ttcgttctta
     5701 tgatattgaa aagttgtcaa ccctttatat cggtggtgga acaccgacag ccctgtcggc
     5761 tccgcaactg gaggtgttac tgaatggctt gactaaaaac ttggatttgt ctgtcttgga
     5821 agagttgacc attgaagcca atccaggcga tttggatgcg gataagatag ctgttttgaa
     5881 aaattcggct gtcaatcgtg tttcgctagg tgtccagacc tttgatgata agatgttgaa
     5941 aaagattggg cgcagtcatt tggagaagga tatttatgaa aatatcgatc gcctgaaact
     6001 ggctggtttt gacaacatct ccattgattt gatttatgct ctgcctggtc agaccatgga
     6061 gcaagtaaag gaaaatgtgg ctaaagccat tggactggat attccccaca tgagtttgta
     6121 tagtttgatt ttagaaaacc atacggtctt tatgaaccgg atgcgacgtg ggaaattgcc
     6181 tctgcctaag gaggaactag aagcggagat gtttgagtac atcattgcag agctggagcg
     6241 agcgggtttt gagcattatg agatttctaa tttctccaaa cccggctttg aaagtcgtca
     6301 caatctcatg tactgggaca atgctgaata ctatggtatt ggtgcagggg catctggtta
     6361 tgtcaatgga gtacgctata aaaatcatgg tcccattcgt cattatctca gtgcggttga
     6421 ggaaggcaat gcttgtatta cagaagatca cctgagtcaa aaggagcaaa tggaagaaga
     6481 aatgttcttg ggactccgca agaaatccgg ggtttccatg gcgcgatttg aggaaaaatt
     6541 tggacagtct tttgctggac tttatggaga aattgtcaga gatttggttc aacaaggcct
     6601 catgcagatt gagggtgacc acgttcgcat gacaaagaga ggtctctttt tgggagatac
     6661 tgtagcagaa cgatttattt tggagtagga tcatgggctt aacttatcaa atgaaaatga
     6721 aaattccttt tgatatggct gatatgaacg gtcatatcaa acttccagat gtgattttgc
     6781 tgtcactgca agtttcaggg atgcagtcga ttgaactggg agttagtgat aaggccattt
     6841 tggaagacta taatctggtc tggattatcg cagaatatga tattgaggtg gttcgtttgc
     6901 ctcgttttgc ggaagaaatt accatcgaaa cggaagcctt gagctacaat cggctttttt
     6961 gctaccgtcg ctttactatt tataatgaag cggggcagga gctcatccat atgatggcga
     7021 cctttgttct catggaccgc gacagtcgaa aagtccatgc tgttgaacct gagattgtgg
     7081 ctccttacca gtctaatttt gataaaaaac ttatccgtgg accgaagtat gagtccttgg
     7141 aagagctgat cagcaaggat taccatgttc gtttttacga cttggatatg aatggtcatg
     7201 tcaataacag taaatacttg gactggattt ttgaggtcat gggagcggat tttttgaccc
     7261 actatattcc caagaaaatc aacctcaagt atgtcaagga agttcgacca ggtggggtga
     7321 ttacatcggc tgttgaacgg actggactgg aaagcaagca tgaaattaca agtgatgggg
     7381 ttatcaatgc ccaagcaatc attacttggc aagaaataaa aaaggcttag ggagaaatag
     7441 atgaaatata aaggctattt aattgattta gacggaacca tttataaggg gaaagaccga
     7501 atccctgctg gagagacttt tgttcatgaa ttgcaaaagc gggacattcc ctatctcttt
     7561 gtaaccaaca atacaactcg cactccagag agtgttaagg agatgttggc tcagaatttt
     7621 aatatcgata cgcccctatc gactgtctac acagcgactt tggcaaccat cgactatatg
     7681 aatgatttgg ggcttgaaaa gaccgtcaat gttgtcggag aagcaggact caaggaagcc
     7741 atcaaggcgg ctggttatgt ggaagacaag gaaaaacctg cctacgtggt agttggtctg
     7801 gattggcaag tcgactatga aaaatttgcc acagcaactc ttgctattca aaagggtgcc
     7861 cattttatcg gaaccaatcc tgacctcaac atcccgacgg aacgcggtct tttgccaggt
     7921 gctggctcac tgattacgct gcttgaagta gcgacacgag tgaagccggt ttatatcgga
     7981 aaaccaaatg ccatcattat ggacaaggcg gttgagcact taggtttgga acgtgaagag
     8041 ttaattatgg ttggggacaa ttatttgact gatatccggg ctgggattga caatggcatt
     8101 ccaacgctct tggtgacgac aggttttact aaggcagaag aagtagctga cctgccaatc
     8161 gcaccgacac atgtggtttc tagccttgcg gagtgggatt ttgatgaaaa ctaaattgac
     8221 cttttggggc tctatgctct ttctcctctc cctctcaatc cttctgccca tttatctggc
     8281 ttggattttc tatcctatgg agattcagtg gctaaactta acgaatcgag tctatctaaa
     8341 accagaaacc attcaataca attttcatat cttgatgaat tatctgacca atccttttag
     8401 tcaggtctta cagatgcctg attttcgttc gtcagcagct ggtctgcacc atttcgcagt
     8461 ggtcaagaat ctctttcatt tggttcagct agtagctcta gtgacactgc caagtttcta
     8521 tgtctttgtc aatagaattg tgaaaaagga ctttttgtct ctttatcgaa aaagtctcct
     8581 ggctctagta gtcttacctg tgatgattgg acttggggga gttttgattg gttttgacca
     8641 attctttact cttttccatc aaattctctt tgtgggagat gatacctggc tttttgatcc
     8701 agccaaggat cctgttatta tgattttgcc agagaccttc tttcttcatg ccttcctcct
     8761 cttttttgcc ctctatgaaa gcttctttgg ctatctgtgt ctgaaaagtc gtaggaagtg
     8821 aaatgaaaga tgtttttaat ttgcatatat aaatatatat atcgtttaaa accatcgtta
     8881 agagtgaatt aaccaaatcc aattgtgtca atcgttcgca aacagcctat ttttcttgaa
     8941 aaaataggct ttttttctaa aaatccctag tcaagcgcct tatttttt
//
LOCUS       DAVKDZ010000076         8884 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000076 DAVKDZ010000000
VERSION     DAVKDZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8884
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.077"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(74..1696)
                     /locus_tag="VWZ30_001764"
     CDS             complement(74..1696)
                     /locus_tag="VWZ30_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9335449.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGTY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLILIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYIKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLAMGLFVFTVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            1802..3247
                     /locus_tag="VWZ30_001765"
     CDS             1802..3247
                     /locus_tag="VWZ30_001765"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEU9335450.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYHYNSVVFDTICYDSRK
                     AKEKSLFFVKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMEHFSVLKEQAEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGHFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGRKESYPGDTAVAENYL"
     gene            complement(3290..3487)
                     /locus_tag="VWZ30_001766"
     CDS             complement(3290..3487)
                     /locus_tag="VWZ30_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9335451.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTKE"
     gene            complement(3588..4196)
                     /locus_tag="VWZ30_001767"
     CDS             complement(3588..4196)
                     /locus_tag="VWZ30_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEU9335452.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKAGLILR
                     ENKRYYLNFSMLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            complement(4221..4733)
                     /locus_tag="VWZ30_001768"
     CDS             complement(4221..4733)
                     /locus_tag="VWZ30_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU9335453.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPAAFFESNHLYDLYVYPEMDIQSVKERACKHFGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            complement(4820..5755)
                     /locus_tag="VWZ30_001769"
     CDS             complement(4820..5755)
                     /locus_tag="VWZ30_001769"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEU9335454.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELVELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            complement(5826..6125)
                     /locus_tag="VWZ30_001770"
     CDS             complement(5826..6125)
                     /locus_tag="VWZ30_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU9335455.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDVRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            complement(6115..6864)
                     /locus_tag="VWZ30_001771"
     CDS             complement(6115..6864)
                     /locus_tag="VWZ30_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEU9335456.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            complement(6919..7278)
                     /locus_tag="VWZ30_001772"
     CDS             complement(6919..7278)
                     /locus_tag="VWZ30_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU9335457.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            complement(7280..8680)
                     /locus_tag="VWZ30_001773"
     CDS             complement(7280..8680)
                     /locus_tag="VWZ30_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEU9335458.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYM
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKLEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
BASE COUNT         2707 a         2028 c         1654 g         2495 t
ORIGIN      
        1 cgctaaaaaa ggaataagtt caagttctcc taaaagattg ttcaacttat tccttttatt
       61 tataaatgct accttacgaa agcttaaatt ttgctcgcag gcgatctgct tgggcttttc
      121 ctattacctt atctaataaa taggtacgca gactcataac catataaagt ccacccccca
      181 tggcaccgac aagagctaca taaaagaagc tccacaaacg tccacttggt tggaagaaaa
      241 atcctaacag ccactggatg gttcctatta acagaaacat gactagggtc agcaaactga
      301 ttaaaatggt tcgcttcaaa atcaccttgc gcttgacacc agttacttta caaatatccc
      361 gatacatcaa gacgttagga atgatgagag caatggttgt tgaaatcaaa ggaccataac
      421 tgtggaagag ggcgatggta ggtagttgca agactagctt ggcaatagaa ccgtagataa
      481 aatagagaac ggccttgcgg ttgcggaaca tggcctgaag cattggagac aagaccatat
      541 acaagcctaa aataatagac tgcaaaactg taaagacaaa taagcccata gccaaactat
      601 ctggcttacc atagaagacc gtataaagag gttctcctac cataaccact ccaaccgttg
      661 ctggtagcaa gaacataaag agtagggtga gactgtcctg aacgagacga gaagctgcct
      721 tcaagtcccc cttgatatag ttttccgtca aaagtggcaa accaacactc ccaatcgaaa
      781 cccctacaga aatcaaaatc atcgtgattt tattaggatt ggctgagaaa taagaaaaca
      841 tgacaaccaa gtcctcatta ctgtagttgg taaaccagct catactattg ataaaggtca
      901 gctgatccaa aatctgaaag agctggatgg cagaccctgt caggataaaa ggaatggctt
      961 ccttaatggt atcaaccaag agacgcttac tgttaatctt atctcctgtt tcaaagactc
     1021 ttttgagtga accttcttgg gcaaggaaat aaatcaagac tgcaaaactg gctaccatac
     1081 cgacaaaggc agcaaaggtt gattgggtaa cggctgctag ataatctcct gaaccgagct
     1141 tcataatgat aaaggttgct aggagcatcc agataacacg aatgacctgc tcagcaattt
     1201 gactcatggc ataaggtttg aggttattca tcccttggaa aaatcctcgg ataacactca
     1261 tagacgggaa aatcaagact ccccaagcca agctttgcat gattaggatc aagtctttgc
     1321 ccacgccaga caagtctgct agccaaggag caaagacata caagactaaa gcaaaaacca
     1381 gacctagtcc tgtcataaag cctaagaagc tccgaatcag ggcaaagcta tgctcttctt
     1441 ctcgcatagt attatacttg gcaacttgct tggccaccgc aactggaatc cccgctgttg
     1501 aaaccaacaa gaaccaagca tagatattgt aacccatggt aaagagacca tttgccttag
     1561 ctgcataagt ccccatccag atgtaccaag ggataatgta aacagccccg agtaggcgac
     1621 tgataaagtt actagccgtt agccaagcag tcccccgtaa catctgagcc tgctggtgat
     1681 tgttttcgtg cgacatagat tcctcattca attttgataa ctaggaaaaa ctccttatct
     1741 tccattataa acttttcctt gttacttgta aatttgcgac tttcggttta caatagaaag
     1801 tatgataaaa attgaaaccg tattagatat attaaagaaa gatggtctct tccgcgagat
     1861 tatcgaccaa ggacactacc actaccacta caacagtgtc gtctttgaca ccatctgcta
     1921 tgacagccgc aaggccaaag aaaagagcct atttttcgtc aagggcgctg cctttaaaaa
     1981 agaatacctt ctttctgcta taacacaagg cttagcttgg tatgtagctg aaaaggacta
     2041 cgaagtcggt atccctgtca tcattgtgaa cgatataaag aaagccatga gtttgattgc
     2101 catggagttc tatggtaatc cacaggaaaa actcaaactc cttgccttta ctggtactaa
     2161 gggtaagaca acagcagcct atttcgccta taacatctta tctcaagggc atcgacctgc
     2221 tatgttgtcg accatgaaca caactcttga tggcgagact ttctttaagt cagcgttgac
     2281 aacccctgag agtattgacc tctttgacat gatgaatcag gctgtgcaaa atgaccgtac
     2341 ccacctcatc atggaagtct ccagtcaagc ctatctggtc aaacgtgtct atggtctaac
     2401 ctttgatgtg ggagttttcc tcaatatcag cccagaccat atcggcccga ttgaacaccc
     2461 tagctttgaa gactatttct accacaagcg tctcttgatg gaaaatagcc gagcagtcat
     2521 cattaacagt gacatggagc acttctcagt cttgaaagaa caggctgaag atcaagacca
     2581 tgatttctat ggtagccaat ttgataacca aatcgagaat tccaaagcct ttagcttttc
     2641 agctacgggt aaactcgctg gagattatga tatccaactg attggtcact tcaaccaaga
     2701 aaatgcagtt gctgctggac ttgcttgtct ccgtctcgga gcaagtcttg aggacatcaa
     2761 aaaaggcatc gctgcaaccc gcgttcctgg tcgtatggaa gtcctcactc agaaaaatgg
     2821 agccaaggtc ttcatcgact atgcccacaa tggggatagt ctgaaaaaac tcatcaatgt
     2881 ggttgaaact catcaaaccg gaaagattgc tctggttctg ggatcaacag gaaacaaggg
     2941 agaaagtcgt cgtaaggact ttggcctcct cctcaatcaa caccctgaga ttcaagtctt
     3001 tctgactgct gatgacccta actatgaaga cccaatggcc attgcagatg aaattagtag
     3061 ctacatcaat catcctgttg aaaagattgc ggatcgccaa gaagccatca aggcggcaat
     3121 ggctatcaca aatcacgaat tagatgcagt tattattgcg ggtaagggag ccgattgtta
     3181 ccaaatcatc cagggcagga aagaatccta cccaggagat acagccgtcg cagaaaatta
     3241 tttataagaa tagaaaaaat caagggaaaa tgaaaccctt gattttttcc tattctttag
     3301 taaaaacgtt tttcaaacct tcaacggcac cttctacagc accttttgcg tcttctacaa
     3361 cgtcttttgc cttagcaact gttttttcaa cagctccttc aagttctgtc ttgctgtctc
     3421 cagtaacttt accaaaacct tctttgacag cgcctgttgc ttgttccaat ttgttttcaa
     3481 gtgacatatg attgtctcct ttagtttatt ccgatatgtg ttaccggtta catatagttt
     3541 ataccgaata ctaaggaaag tcaaacaatg tgctcagaaa caagaaatca cgctaagtag
     3601 aaagttaatc tctccttatc aaaatcgata gccaactcat actttccatc ttcattttgc
     3661 acaatatagc ctaagacaac caaactgtcc acaaagatat cacggcattt ctgcataagc
     3721 tggtcttttt tgagaaattt caacaaaaaa gccgtcatat acttgagggc atactcagga
     3781 ttaacatctc ctaaaatgtc atagagctcc tgctgttttt ctgtcaaagg atactgctgt
     3841 ttgaccttgt aaaaataatt ggacagggtc attttaatgc gcgcaaagtc cgtcttttca
     3901 actaaaatag ctgcattgat ttgattgcgc aattccgtct caaaactctg ctccaacaag
     3961 gcttgataga ccggactagc ttctctgaca aaaatctctt gatccagttc aagactatca
     4021 agtgattcaa gcatagaaaa attgaggtaa tagcgtttat tttcacgtag aatcaagcct
     4081 gcctttatat actcttccat gagtttatca actgcaacat ctggaaattg agccttaatt
     4141 tcccgtagaa tcacatcctc atactggtcc agatatcgga tcaattctcc aaaaatggct
     4201 gtctcgtcaa acgagatgga ttaaaaatct gaatcatgaa gattcctttc tttacattgt
     4261 aacagaggcg atgctgccaa ctgactagga ttggtaccag cttggttcag aggaacaggc
     4321 agacctagtt ctatgtaata ctccctccaa aaatcactaa tctcctgctc cccatctcca
     4381 aatttcatag gaatagtttc aaaaataggt aggatttctg ctatatactg ggagcagtag
     4441 aaaccagctg catctggata gaaagaagca ttgtagggtg ctccaaaatg tttgcaagct
     4501 ctttccttca ccgactggat atccatttct gggtaaacat agaggtcgta caaatgattg
     4561 gactcaaaga aggctgccgg ttcttgacag acaacaccag cctgtccact agcatgataa
     4621 atcatcccat ccaaataaat ggcaacatgg ctatagttac ctgtggaagt ctggatggcc
     4681 tgtcccatgt ctgacccatc tctcacaaaa atcaaatcac cattttctaa catacttcac
     4741 tcctagaaaa aaaggagccg aaactccttt cgatataagg caaactagcc tttccgattt
     4801 tgagctgaca cccaaaatct tacgcattaa agctttcagt caattgaggt accacttgtt
     4861 tcttacgtga aacggcacca gcaaggaagg catgattgtt ttcaagtttg aagttgaaag
     4921 ccgcttcgac cttgtccata ttggcaccaa gagccaagat ttctgagttt gagttgacga
     4981 tatctgtaat catcaagaca aagtcagagt agccgtttga ttcgttggca gcttgcattg
     5041 cagcttcaat ttctgcttgg cgttccaaaa cttcagcgat gtcaactgtg ttcacttggg
     5101 caacacggac attatttccg ttgagttcaa aagtcttagc atcgatgtca atcaattctt
     5161 cagcagattt gctagccaag ttggtaccag ctttcaacat tgccaaacca tattcttcca
     5221 agttcacacc agccaattca accaattcag gagcaatgat tttatctgtt gggtgtgttg
     5281 ttggtgattt caaaagaagg gtatctgaaa tcaaacctga aagcatcaaa cctgcaatct
     5341 ctttaggcac agctacacca tgttctttga acatacggta aacgattgaa gacgctgatc
     5401 caactggctc caaacgcatg taaagtgggc ttgcagtttc aaagttagcc acacggtggt
     5461 ggtctacaac accgtaaact tctacttcag cgatatctga tacagattgt tggaattcat
     5521 tgtggtcagt caagataact tgctctgccc cctctgcttt ggcagaagtg ataacacgtg
     5581 gtgcttccac accaaaatag ttcaagacaa aggctgtttc ttcatttgga gttccaaggg
     5641 caacagcttc cgtatccaaa ccgtaagctt cttttgcaag gtaggcaaaa gctacagatg
     5701 acccgatggc atctgagtct ggattttggt gaccaaatac tagaatcttg gacatggtaa
     5761 tacctcattt tgtttatttt ctttattgta gcattttttt cgctttttga caaaggtaag
     5821 aggagtcaaa ggactcccca cgattcttca aaataactga gtaaatttct atcttgattt
     5881 tcagataaaa attcttcctt ctgtggcctc ttcttacgct tgagaagagc ctcagccgac
     5941 atggcttctt ccttactggc aaaaccttga gcatagataa gtttgactgg caagcgtgct
     6001 cgtgtatatt tggctccctt cccactattg tggatagcga ggcgtcttct cacatcagtc
     6061 gtatagccta tatagtagga tccatcacga cactccagaa cgtacatata agccttatga
     6121 tccataataa atctcttcga tttcgggcgt ataagagcca tcatcattgt ggacaatcaa
     6181 aggaggtaag accttaaagc cacttgttga gccatccttg atcgcctcaa tcaaaagcat
     6241 attggcttcc ttttctcttt ttggataaac aaactgcagg cgcttagggg ctagattatg
     6301 tcgttttaac gtatccaaaa tatccagaag tcgatcagga cgatgaacca tggccaaacg
     6361 cccattagac ttgagaatac tctgggcact acgacagatt tcttccaaat tagtcgtgat
     6421 ttcgtgtcga gccaagagat aatgttcact ctcgttcaga ttagaataag gatccacctt
     6481 gaaatatggt ggattacaca aaatcatatc caccttactc ccctgaatgt gagcaggcat
     6541 atttttcaaa tcatcgcaga tgacctgcat ttgctcctct aatccattca aacggacaga
     6601 gcgttcagcc atatccgcca aacgctcctg aatctcaaca gacaatatct gtgcttgagt
     6661 acgagtgcta gcaaaaagcc ccactgctcc attcccagca cagaaatcca caatcaaccc
     6721 cttcttagga aaacgtggaa atcgtgataa gagaacacta tccaccgaat agctaaaaac
     6781 ctctctattt tgaatgattt tgatatctgt cgaaaagagc tggttaatgc gctctcctga
     6841 ttttaataat tgttcttctt ccatggtcct attataataa atttatatta acattacaaa
     6901 aaaatataaa actctaaact acttcttctt ttttaaatgg tgcagagctt ctccagtcca
     6961 gattggtagc attcgtcgaa agggagcaaa accgtagtta aagcggtcgc ttgaaaagcg
     7021 tctccgtcta ggaaactggt acttttcttc ctccaaagtg cggatagaaa gactggcttt
     7081 ccccgtaaat tcatctaaat ccactacctg aacttgaacc tcttcatcga ctttcaaggt
     7141 ttcatgaata ttttcaataa atcctgtccg aatttctgaa atgtgaatca gccccgtatc
     7201 acccgtctct aactcaacaa aggcaccgta gggctgaatc cctgtaatac gcccctttag
     7261 cttatcaccg attttcatct tagtcctcga tttcaatagt ttcaattaca acatcttcaa
     7321 ctggcttgtc catagctcct gtctcaacag cagcaatggc atccaagaca gcgtaagatg
     7381 cttcatcagc taactgacca aaaaccgtgt gacggcggtc taggtgaggt gtcccacctt
     7441 gattggcata gatttctgca atcggttctg gccaaccacc acgagtaatt tctttcttcg
     7501 aataaggtag gtgttggttt tgcacgataa agaactggct gccgttggta tttggaccag
     7561 catttgccat ggaaagagca ccacggatat tgtaaagctc ttctgagaat tcatcctcaa
     7621 aagattcgcc gtagattgac tcgccaccca taccagttcc agttgggtct ccaccttgga
     7681 tcataaagtc cttgataata cggtggaaaa tgacaccatc atagtagcca tcttttgaaa
     7741 gagatacaaa gttagccact gttttaggag catgttcagg gaaaagcttg atacgtaagt
     7801 ctccgtgatt ggtcttaata gtcgcaagag gaccttctac tgtttcaatg tctacttgtg
     7861 gaaaatgcaa ttctttttct accataccaa atacttctaa ggcatcaaaa atgccatctt
     7921 cttctaatgt ttttgtaata taatctgctt tttctttgat tttatcatga gaaattccca
     7981 tggcaacgct gattccagca taatcaaaga gttccaagtc attgagacca tctccaaaaa
     8041 ccatgacctt ctctagtttc aagccaaggt gttccacaac cttttccacc cccgtcgctt
     8101 tggagcctga aatcggcaca atatcagacg aatgttgatg ccaacgaacc atgcgaagtt
     8161 tgtctgagag actgtcaggc aagtgcaagt catctccctt atcttcaaaa gtccacatct
     8221 gatagatatc ttctttttca tggaaatcgg gatctacatc taagtcggga taaattggat
     8281 tgatagcttc actcatcata tcggtgcgag tcgacaactt ggcatcatga ctcccaacca
     8341 agccatactc aattccttct tgcttagccc aagacatata ctcctcaaca tctgactttt
     8401 caatctgatg ctgataaatg acctgacctt ttttatcttc gatataagcc ccattcaaag
     8461 ttacaaaaaa gtcaggcttg agatcacgaa tctctggaac aacaccaaaa atgccacgtc
     8521 cagaggcgat tcctgttaaa attccttttt cacgcaactg tttaaaaaca gtgggaattg
     8581 tagttggaat aaaccctgtc tttgaattcc gcaatgtatc atcaatatca aaaaagacaa
     8641 tcttgatctt ctttgccttg tatcttaatt tcgcgtccat ctcactacct ctttcaatct
     8701 aactctttcc attatatcat aaagtaggca aatcccctat tttcaaaaag tttatcattt
     8761 ttattttaat ttcttggatg agaaaagaga catatttatg aaaaagtcca accaaattca
     8821 actcgacttc tcattccaga actcgtaaaa aaaaacctaa tatccagatt ttagacatca
     8881 tcaa
//
LOCUS       DAVKDZ010000077         8815 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000077 DAVKDZ010000000
VERSION     DAVKDZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.078"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(165..1343)
                     /locus_tag="VWZ30_001774"
     CDS             complement(165..1343)
                     /locus_tag="VWZ30_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335459.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLPLDQQLEEEEKLLRKVENLTKKV
                     QKKEKIKVNKSYFDERLLKWLWEKGVFKPNRGNQFSPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERVYQDYFGFTETLTQEERKWAKIFFGKNRTERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNPYFRELPEEKSRLARLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKESLQADHLSSAQESWIEGILLNCIKPFLERLVI
                     "
     gene            complement(1361..5011)
                     /gene="addA"
                     /locus_tag="VWZ30_001775"
     CDS             complement(1361..5011)
                     /gene="addA"
                     /locus_tag="VWZ30_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEU9335460.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGHGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FAKLGQLSDQITILDYQERYHGDTWKLAKTFQTFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILEKFPQVREAYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAKNPHEGKLILLKENFRSSSEVLSATNDVFEGLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKVEFLLYDKDDTGEEEEGQAETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIGMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEAKEYPAAK
                     NGKLNSTTRLQARNFQDWLWAVSKVFTKDKLNFSYRFVGEDQLTREAIGELKTKSPLQ
                     DSSQADNRQSETIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVDKKISFDLPDFSSKEKVTGAEIGSATHELMQRIDLSQQPTLASLTETLK
                     QVQTSPAVRDKINLAKILAFFDTALGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYEGRIVLFDYKTDRYDEPSQLIDRYRGQLALYGEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            complement(5008..8283)
                     /gene="rexB"
                     /locus_tag="VWZ30_001776"
     CDS             complement(5008..8283)
                     /gene="rexB"
                     /locus_tag="VWZ30_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEU9335461.1"
                     /translation="MKLLYTDIRTSLTEILTRKAEELVAAGKRVFYIAPNSLSFEKER
                     AVLEYLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLRYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQIFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQTTEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELLHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLIAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            complement(8483..8641)
                     /locus_tag="VWZ30_001777"
                     /pseudo
     CDS             complement(8483..8641)
                     /locus_tag="VWZ30_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
BASE COUNT         2374 a         1878 c         1818 g         2745 t
ORIGIN      
        1 cagtataatt taattatact attaatttac aaaaaaatct taaaattgag ttgcgctttc
       61 atactttgtt ttacgtgtaa aatacgaatg cgcagggttt gaatttctta tattcttttt
      121 ggataaaatg taacctacca tgaaatttcc acggcaggtg ttattcatat cactaatcgt
      181 tctaaaaatg gtttgatgca gttgaggaga atgccttcta tccagctttc ttgtgctgag
      241 gagagatgat ctgcttggag gctttctttc agaaaatctc gaacttgttc tggtgcgatt
      301 tcaaactcaa aagctttcat tttatagaaa aagtcgataa gatgatctga tagatattca
      361 gttgaaaatg gaacttctcc actttttcga tattctaata agagccgagc caatctggat
      421 ttttcttcag gaagctcacg aaaataggga ttgaggagcc aggtctgctt ctgctttctt
      481 tcaattggat cctgactggc tattcgttgg tctttttcca actctttttg gtattgtttg
      541 gccttgatag cccgttctgt tcgattttta ccaaaaaaaa ttttcgccca cttgcgttct
      601 tcttgagtca gagtctctgt aaagccaaag taatcttgat agacacgctc tgctgggccc
      661 atggctagga ctagattgtc tgcatattgc ttggcgattt tatccctctt cttgcgctct
      721 ttctctgcct ggatacggag ttcttgctcg taatcaattt tctccttgcc tagctttaca
      781 agatagagtt ggtcatccga tttcccaagt aaaaagggtt tgatacactt ttcaagaact
      841 tcctccatac gagccttctt cttgggctct gccttggtcc aacttcctcc ttgaaagact
      901 tctaggaaga gttggtagtc tctctcaggt gagaactgat tgccacgatt aggtttaaaa
      961 acaccttttt cccagagcca ctttagaagc cgctcgtcaa agtaactttt attgaccttg
     1021 attttttcct ttttctggac ttttttggtc agattttcaa cctttctgag cagtttttct
     1081 tcctcttcta gttgctggtc aaggggcaat cgatgaaaat gacgaacaca gtcgctacca
     1141 atcggaaaga ggcgttggcc tgtgacacca ttaaagagtt cgtaggcgta cttgatggca
     1201 tttccacaga cacaattact acggccgatt ccgttaaaaa tcaaggaaac ttcattccat
     1261 tccttggtag cttgttccca agtatcagct tttgaagcct gtaaaaccgc atcgtgcagg
     1321 gattttctaa ctggaagtgt catgaggtct cctttctaga ttatactttt acaacttgaa
     1381 cctcatcttt accgagtaaa atcaagtatt tttcaatatt ttcaatcgaa taggctcgtg
     1441 ataaagcctc tccgtatagg gctaattgac cacgatagcg atctatgagt tgacttggtt
     1501 catcatagcg gtctgtctta tagtcgaaca gaacgattct gccctcgtaa agcagatagc
     1561 catcaaggat accacgaaca acgaaatctt cctggctctt ttggtctcgt ttgagcatgg
     1621 agaaaggttg ctcgcgatag agatggtcgg tattagcaag aatttcctga ccgagtgctg
     1681 tgtcaaagaa tgcaagaatt ttagcaagat tgatcttgtc tctgacagct gggctggttt
     1741 gaacttgttt gagagtttct gtcaggctag caagcgtcgg ttgttgactg aggtcaattc
     1801 tctgcatgag ttcgtgggta gcactaccaa tctcagctcc tgttaccttt tctttgcttg
     1861 aaaaatctgg taaatcaaag ctaattttct tgtctactga ctgaccttga cctgcaatct
     1921 cgacaccttc catatccata acaggttcgt aaaatttctt gatttgactt ggggtttgaa
     1981 cactaggaag ttcaatagct gcacggtgaa gagtattata aacttccact tctttcagca
     2041 tttccagagc ttctttaata gtttcagact ggcgattgtc tgcttgggag ctatcttgga
     2101 gaggactctt ggttttcaac tctccgatag cttctctggt caactgatct tcgccaacaa
     2161 aacgataact aaagttgagc ttgtccttag taaacacttt actgacagcc caaagccaat
     2221 cttggaaatt ccttgcttgc agtctagtag tgctatttag ttttccattt ttagctgctg
     2281 ggtattcctt ggcttccaac ttttcacgag aacccttacc gacaagatag agctttttct
     2341 cagccctcgt catggcaaca tacagcagac gcatttgctc agaatagctt gctagctgta
     2401 attcttcttc gttctgccta taggtcagac taggaatgga gagtttgatg gttttaggat
     2461 agtggtcttc tactgcccct gtctccatct tggcaatata tttgacacca agaccatttt
     2521 gacgactgag aatgacttct gacatagagt cttgcttgtt gaaatcttga tccatattga
     2581 ggataaagac ataaggaaac tccagccctt tactcttgtg gatggtcatg agctctactg
     2641 catcttttgg cggtgcgacg gccacgcttg ccaaatcgtg ctgggcttct aaaacttggt
     2701 caatcatacc aataaaacgc gacaaacctt tgaaattgct cttttcaaat tgatcagcac
     2761 gcagtgctaa ggcatagaga ttggcctgcc tagcaggacc attcggcaaa gccccaacat
     2821 agtcataata aaaacggtcg ttgtaaatct tccaaatcaa gtcatagaga gagtgggttt
     2881 tggcatacaa gcgccaagaa gctaggatat ccatgaattg ctttagtttc tcagctagag
     2941 ctgtgtgaat taagcctttt tgactacttg ccattttttg tgcattgacc agtttctcat
     3001 agagattttc gtggacttta tcctctgctt tctgaaggga caaacgtgct agctcatcct
     3061 catcgaaacc aaacattgga gacttcataa gggcaaccaa ggcgtagtct tgcaggggat
     3121 tgtgaatgac acgaagggtg tctagcatga cttgcacttc tagggattgg agataattgt
     3181 tttgctctcc gtcagttttg acaggaattc cgtactcaga cagggcgagg agaatctggt
     3241 cattacgact gcggctggag gtcagaaggg caatttcttt aaaggcaaca cctttttctt
     3301 gatgaagttt cagaatctcc ttgataacta agcgcatttc gcctgttagt ttcgtttctg
     3361 cctgcccctc ttcttcctca cctgtgtcgt ccttgtcata gaggagaaat tctaccttgt
     3421 tgtctggatt gggagtcagt ttggtattgg caaaaacaag ctggtgcatg ttatcatagt
     3481 tgatttcgcc gacctcttga tccataagac cttcaaaaac atcattggtt gctgatagca
     3541 cttctgaact actacggaaa ttttctttaa gcaaaatcag cttgccttcg tgtggatttt
     3601 tcgcatagcg ttgaaatttt tcattgaaaa tctgcgggtc tgcctgacgg aaacgataga
     3661 tggactgctt gatatctccc accataaagc gattgtgccc attggacaac aattccagca
     3721 tccgttcttg aatatggttg gtatcctgat actcatcgac catgacttcg tggaaacgct
     3781 cctgataagc ttcacggact tgcgggaatt tctctaaaat ctcaatggtg taatggctga
     3841 tatcagcgaa ttcaaaggca ttttcctgac gtttacgctg acgataagcc tccacaaaat
     3901 cgctcataaa ggtttggaag gttttagcta gtttccaagt gtctccatga taacgttctt
     3961 gatagtcgag aatggttatc tggtctgata attgtcctag tttagcaaac tgggtctttc
     4021 tctcttcgtt gtaggcatca gccagaggct tcaaatcagc cttacgactg gcattagtaa
     4081 gagctcgacc gtttttctcc ttagagatgg cgacaacacg cgcaagcact gcctgataag
     4141 cctgactatc ggactcctga tttagggagc caatttcatc cagaattaac tgaacatttt
     4201 ctaaataggc agcctttgca aactccttgg catcgttatc cagatggtaa cggaaaaagc
     4261 tttccaaatc ccaaagggct tgtttgattt gctcagtcag tttttctttt tcactggtaa
     4321 aatcagcttt ctcaaagcct ttgaggaaag attcactcag ccacttttga ggattactgg
     4381 tggattggag gaagtcatag attttataga cctgctggcg cagaccccgt tcgtccttgc
     4441 catgcccagc aaagtttttc agcaaatgac taaaggtctc tttctgttta ccttggtaat
     4501 gcgcttcaaa gacctcatga aagacttcgt tttcgagaag aagttgctcg ctttggtttt
     4561 gtaaaatacg gaaattaggt gcaatatcaa gcagataacc atgtttgcca aggaattttt
     4621 gtgtgaaaga atccatggtt ccgatggcag cgttgggtag gtctgccaac tggcgaccca
     4681 agtgttgttt gaggtcgaca tcatctgttt cttggatttt cttgctgatt tttttctcta
     4741 aacgttcttt aagttcagtt gcagccttga cggtaaaggt tgagataaag agttgagaaa
     4801 tttcgacacc acgcgccaat tggtccagaa tgcgctctgc catgacaaag gtctttccag
     4861 aaccagccga tgctgagacc aggatattct gaccagaagt gtagatagct tcgatttgct
     4921 cggcagtttt cttctgttcc ttgctcgaat ttgcttctgc ttcttgcagt ttttgaatct
     4981 cctcctcact taaaaaggga ataagcttca tcgattcaac tcctctctta ttttttcaag
     5041 ccaagcttgc ttgagttttt ctccgaccag acgcttgcca tcagctaggt ccaacttttc
     5101 taggaaacgg gcttggccca gatggtaatt ggcttcaaag cctgtaatag cctgatgttg
     5161 ctggacgtat ggggcaatgc ttctgccatt ttcagtataa ggattgatgg cgaaccggcc
     5221 tgctaaaatc ttctcagcag ctttcttgta aagataggca ttgtagtcca gtaggagctg
     5281 aaattcctca tctgtcagtt gattagcctt gtttttgtta taaaattcgc ctaaataact
     5341 gctttctttt tccaagaaga gcccttggta tttcatagat ttgctggctt ctaccactgc
     5401 tcctgccaga ctttttaccg ctatcagaga ttggacaggt tcagccattt ccaagtacat
     5461 ggcgccgaaa aagttctgct ccccttctct ttttagggca gcaagatagg ttggtaactg
     5521 agaattgagc ccattaaaga aatgaggaaa ctggaactga gtcagactgg atttgtagtc
     5581 tactactcct atcgctccat tagctttcaa acggtcaatc cggtccacct tgcctcgtac
     5641 aaagacactg cgtccattgt ctaattgaat aaaggcttgg tcttttccac caaaatttgc
     5701 ttcttctttg atggtttcga tggctggatt gtgtcggaga atatgtccag ttgtccgtgc
     5761 aacatcaagc aaaacttcct tggtaaactg ggcttccaaa ctttcttgat aaatagcttc
     5821 aaattcgcgt tcttgactgg tttcttgaat agcttgttct agacgttggt caaaggaatc
     5881 ttcattaggc aactgcaagg cgcgttcaaa gatacgatgc aagaaattcc cgtgactacg
     5941 ggcatcagga cgcaaacgta attcctcctg caagcctaaa acgtagcgta ggaaataact
     6001 gtattcattg cgataaaact ctgtcaaacc cgacgtagac aggtaaaact cctgtttggc
     6061 aggatagaga gcttgcaagg tgtccttggc taaggtcttg ctgcttggac tggttgggat
     6121 ggctggattt tccagacctt gcagatctag ttttttacct atgacacgcg acagaacctt
     6181 gacaaaagtc aaatcttgct cagtatcgct catctcaccc tgctggtgat aggcaaccag
     6241 actagacaaa agactgtgat aggaccccat atcctcctta gacagtcctt tgtgattcat
     6301 cctcttctct ctccgcctaa atccaaaatg gagcaactct tgaagatagg cagattcctt
     6361 actttcactt tcgttaaaaa ggcttggagc cgacaagaac aactgcttac gagcagaatt
     6421 gaccaaggaa agcatagtgt agcgattttt cttgagattt tcactgctgg caatcagtaa
     6481 ttgaacgcct tcttcggtcg tttggtttag gttttgcctt tcttcatctg tcagaagact
     6541 ggtgttttga gaaatttttg gtaaattgtc ctgagttagt ccaatagcat agacaaagtc
     6601 agcagtcaat ggtgcaatca aatcgtaact ctgcaccaga acagtgtcca ctgttgctgg
     6661 aatggtacgg tattgggaca aactcattcc agaatggagc aaggctagga agtcttccag
     6721 actaacctgt gaaccagcaa aaacagtcgc aaattgttct aaaacatggc agaaagcctt
     6781 ccaaacttcg gcttgtcttt cctgttctac agcttccaaa gtggttgtca aatcttgtaa
     6841 ctgcttggtc acagctcctt cttttagaaa gacactccat ttttgtagga ggttttcagc
     6901 cttttgtttt cggctggcaa agagggtttc aagaggtgct aaaattctca ggcggaggac
     6961 attcaaacgc tcaagattaa attttccatg gtgggatttg gtgaagattt gctgaaaggc
     7021 tggcaagcca ttgataccaa gatagcggat atattgctca aaagcatcaa tatcagactg
     7081 actgaggtca gtatacaagt cagttctaag aagattaatc aaatcctcct gacgaaaacg
     7141 gtaacgtttt aaagctaaaa tagactcgac aaactgagtc aagggatgat gagccatggc
     7201 ttcgcttcta ccaagataaa aaggaatctg atactggtca aaaatggttt tgagagataa
     7261 ctggtaagaa gctacatccc ccaagagaat acgaaaatgc ttgtatctca ggtctgagtt
     7321 ctcatgtaat ttctgacgaa tactacgggc tactagctcc aactcctcct tttgcgtcaa
     7381 acaagaccag atttgtaaat tttcacggtc tttctcatcg acatccaaag cgagttctga
     7441 aaagtcataa gaagactcca acaaacgaga ggccttgtca aaactatcca tcttctcatg
     7501 agtttgagaa cagtcctgag caggcgtttg gtatttagaa gccagatgat ggagaaattt
     7561 tacgctggct tggtagagat tgccctcgct aaaaggactg gtataggctt tcttactagc
     7621 ataagccccg ataacaatct caacaccttt gccgtgaagt aagtccacaa cccgctcttc
     7681 ctcagcagaa aaacgagtaa agccgtcaat gaccaaggcg atttgattaa aatcactact
     7741 taccttgtca ttctcaatag cctcaatcaa atgggacaac tgacttccct gggctaactg
     7801 accttgatta agataggctg ttactttctc aaaaatcaag agtaaatccg ccctcttatc
     7861 ctcatctgtt aaattctcca agtccaaaaa actcatctga gatttggtca tctcatggta
     7921 aagctcaatt aactgctgga tcaattgagg atcctgctta atagcgccat aaacacgcaa
     7981 gtccttggga tcgagttcgg caaggcattt gtaaaaggcc aacccaagac cgatatcatc
     8041 aagagtagtt ttagctggta aatcattcaa gaccagatag cgagccattt gagcaaagcg
     8101 cgtgacggta atcgaaaaag aagcctgctg ggacaagtat tccagcacgg cgcgttcctt
     8161 ttcaaaagaa agagagttgg gggcaatgta gaagacccgc ttgccagctg caactagctc
     8221 ttctgccttt cttgttagaa tttctgtcaa agaagtccga atatcagtat aaagtaattt
     8281 catctcagcc tcgttggaat ttttcatcac cctatattat accatgatta tcctcgtaaa
     8341 tctgttaaaa tatttaggcc atcctttctt ttcttcatca tctgctaaat cttaaatact
     8401 tagctttact tgtattagat agaataagtc tggctactga aaatcacata ataaaaaagc
     8461 ctcggtaaca aggctttgag ttttatgatt gtttcttatt aggtacggaa tacacttcaa
     8521 tgtgttgtcc cagtatctta atgtcgactg gtagattgtc tgatttatca ccatcaacat
     8581 cggactctaa ttcgatatca gaagaagttt taatattacg tgcctttata tattcaatat
     8641 tcttgataga atgattgaac tatagcaaat tgaaactata atagtacacc gtggatgcta
     8701 aaatatttct agaaattaat ttgatttccc taatcaagct gttcgtatct tatttcaatc
     8761 tactatagta aaatgaacca aaaatagtac ataatgtggt ataatcgtct tatgg
//
LOCUS       DAVKDZ010000078         8815 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000078 DAVKDZ010000000
VERSION     DAVKDZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.079"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(113..829)
                     /locus_tag="VWZ30_001778"
     CDS             complement(113..829)
                     /locus_tag="VWZ30_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9335462.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            1177..2964
                     /locus_tag="VWZ30_001779"
     CDS             1177..2964
                     /locus_tag="VWZ30_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU9335463.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPASLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEERGVTCPLFTSDGPWRATLKAGTLIEDDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHCYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            2961..3437
                     /locus_tag="VWZ30_001780"
     CDS             2961..3437
                     /locus_tag="VWZ30_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9335464.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            3465..4370
                     /locus_tag="VWZ30_001781"
     CDS             3465..4370
                     /locus_tag="VWZ30_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9335465.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            4357..5172
                     /locus_tag="VWZ30_001782"
     CDS             4357..5172
                     /locus_tag="VWZ30_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9335466.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            5179..5571
                     /locus_tag="VWZ30_001783"
     CDS             5179..5571
                     /locus_tag="VWZ30_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9335467.1"
                     /translation="MTKSLILVSHGRFCEELKGSTGPQDNIYTVALLPEDGPEEFTAK
                     FEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINASLTGA
                     DADYKSRAAESIVKVNDLLADFDDDEDE"
     gene            5869..7035
                     /locus_tag="VWZ30_001784"
     CDS             5869..7035
                     /locus_tag="VWZ30_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU9335468.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDC
                     NLLLLQPAASNDAGFAMTSSFMSMMLTALLVFDPIEFAVKSERFEVVSSLARKVLDNA
                     EDVKELVDLDFKRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INEDTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            complement(7101..7178)
                     /locus_tag="VWZ30_001785"
                     /pseudo
     CDS             complement(7101..7178)
                     /locus_tag="VWZ30_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            7334..8521
                     /locus_tag="VWZ30_001786"
     CDS             7334..8521
                     /locus_tag="VWZ30_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein C"
                     /protein_id="HEU9335469.1"
                     /translation="MKKIVFASALALTLAGAVLTNDVFANDRLVATQSTDSNVLTSEV
                     LKPSSGNVLVGIKGEFLPPHQQSILDAINKIRKEAADEGLVDKYVPVKWSVDHEKTAF
                     VRAAEVSVTLKAERLSSKNNWTAFPSGNSLSGEALDLNPDGFLKAIENWHAEKANYVA
                     KKKDKTSKEFSFYYENLINPKFTYVGLAAFKNAASPQKAATVALALGTTTSSEELAGG
                     YGSAVQYTEVTASNLSTVKSKAMVVETPLKDFRKSTSDQSGWVQSNGKWYFYESGDVK
                     TGWLKTGGKWYYLNDLGVMQTGFVEVDGSVYYLSNSGAMFTGWGTDGSRWFYCDGSVA
                     MKTGWYKENGTWYYLDEEGIMKTGWFKVGQHWYYANGSGALAVSTTTPDGYRVNANGE
                     WVS"
BASE COUNT         2501 a         1605 c         1946 g         2763 t
ORIGIN      
        1 tataaaatat tccacaaatt atagaatctt ccaaaaataa gtaagcgcta cctttttggt
       61 gtggtataat aagcatagaa aaagcccgag cgattaggct caggctttct tcttagtgat
      121 cacggtcaca tgagatgaat ttaatcttgt agtaatcaga tcgtttgtaa gtttcactgt
      181 attctaaaac ttggccagtt gattcgagtt tggtgatttt agtttgtagg acagtaggga
      241 attgttcatc gactccgagg actgaagccg catgttctgg agttggaaag actatttcgt
      301 tgatttcttc aaagtgttca tcattcatgt gaatgtggta gtctaacttg aaacgattat
      361 agatagaact atagtattca aggtttggat aatttgcatt gatatattgt tctgggatgt
      421 aggatgtatg gtagatataa acgacaccgt ttgattcgcg gatacgttca atcttgtagt
      481 agaattgatc gccgcgtaga cccaattttt ccaagtaaac aagcttgttt ccgcgttcaa
      541 ttgaaagaac agttacctta tcatctttag cattgaagag ttcaatatct gaaaactcta
      601 caagcttgtg tttgcgtgca cgtgaaacga aggttccttt tccttgttgg cggacaatat
      661 agccatcttt ggcaaggtcg tttaaggcgc gaacaactgt gatagagctg acatcgtaca
      721 ttgaaatgag ttctgcttca gtgtaaaatt tatctccact gctaaactgc ccagagatga
      781 ttttattttt taattcgtct tttatgtatt gatatttggg aattgccata tttttacctc
      841 tattttcctt ctccatagaa aattttacca taaaagcgaa aaaaatagta gtcttttgaa
      901 taaaaaatta gaataatagt ttgaaaaatt aagcgatata gggattgctc atattttatc
      961 aatgtattct atacaaaatt attttaggtc ctatattccg aatttaaaga gcgtttgaaa
     1021 tgggtctaaa aatgtttgaa actaattgaa tgtgtgtata atagtaaaaa tgcagtaaat
     1081 atcccgacaa tttctattga caattcaaac agattggttt ataattaata taacaacaaa
     1141 tgaaagcgca aactttcgcg gtcggaaggt agttttatga cacgatttga gatacgagat
     1201 gatttctatc tcgatggaaa atcatttaag attttatctg gtgccattca ttattttagg
     1261 gttcctccag aggattggta tcattcgctc tataacttga aggctcttgg ttttaatacg
     1321 gtagagactt atgttgcttg gaatttacac gagcctcgtg aaggtgagtt tcattttgaa
     1381 ggtgatctgg atttagagaa atttctccaa atagcgcagg atttgggtct ctacgcaatt
     1441 gtgcgtccgt ctccatttat ctgtgcggaa tgggaattcg gtggcttacc agcttcgctc
     1501 ttgaccaaga acatgcgaat tcgctcatcc gacccagcat atatcgaggc agttggtcgc
     1561 tactatgatc agttattgcc aagactggtg cctcgtttgt tggacaatgg tggcaatatt
     1621 ctcatgatgc aggttgaaaa tgagtatggt tcttacggag aagataaggc ttacctgaga
     1681 gcgattcgac agttgatgga agagcgtggc gtaacctgtc cactctttac atcagatggt
     1741 ccatggagag caactctgaa agctggaacc ttaatagaag atgatctctt tgtaacaggg
     1801 aactttggtt ctaaggcacc ttacaacttt tcacagatgc aggaattctt tgatgaacat
     1861 gggaagaaat ggccactcat gtgtatggag ttctgggatg gttggtttaa tcgctggaaa
     1921 gaaccgatta tcacacggga tcctaaggaa ttggcagatg cagttcgaga ggttttggaa
     1981 caaggctcta tcaatcttta catgttccac ggtggtacaa actttggttt catgaatggt
     2041 tgctcagctc gaggaacttt ggacctgcca caagttacgt cttatgatta cgatgccctt
     2101 ctggatgaag aaggaaatcc aactgctaaa tatcttgcag tcaagaagat gatggcaaca
     2161 catttttcag agtatccgca gttggaacca ctctacaaag agagtatgga gttggatgct
     2221 attccactag ttgaaaaagt ttctttgttt gaaaccttag atagcttgtc aagtcctgta
     2281 gaaagtctct atcctcaaaa gatggaggag ctgggacaaa gttatggcta cctactttat
     2341 cgaacagaaa caaactggga tgcagaagaa gaaagacttc gtatcattga tggtcgagat
     2401 agggctcagc tgtatgtcga tggtcagtgg gttaaaactc aatatcagac agagattggg
     2461 gaagatattt tttatcaagg taaaaagaaa gggctatcta ggttagatat cttgatagaa
     2521 aatatggggc gtgtcaacta tgggcataag ttcttagcgg atacgcaacg taagggaatt
     2581 cggacagggg tctgtaagga tctgcatttc ttactaaact ggaaacacta tccactccca
     2641 ctagacaatc ctgagaaaat tgatttttca aaaggatgga ctcaaggaca accagccttt
     2701 tacgcttatg actttacagt cgaagagcca aaagatactt acctagactt gtctgagttt
     2761 ggtaagggag ttgcctttgt caatgggcag aatctaggac gtttttggaa cgttggccca
     2821 actctctcac tttatatccc tcattgctat ctcaaggaag gtgccaaccg catcattatc
     2881 tttgaaacag aaggtcaata taaagaagag attcatttaa ctcgtaaacc tacactaaaa
     2941 catataaagg gggaaaactt atgacaattg taggatgccg tattgatgga cgtttgatcc
     3001 acggacaagt agccaatctt tgggctggaa aactaaatgt ttcacgcatt atggttgtag
     3061 acgacgaagt tgtcaacaac gatattgaaa agagtggttt gaaacttgcg acaccaccag
     3121 gtgtgaaatt gagtattttg ccagttgaga aagctgcagc caatattctt gctggcaaat
     3181 acgatagcca acgtctcttt atcgtggctc gtaaaccgga ccgcttcctt ggtttggtag
     3241 aagcaggtgt accacttgaa actcttaatg ttgggaatat gtctcaaaca ccagaaactc
     3301 gttctattac acgttctatc aacgtagtag acaaggatgt ggaagacttc cacaaactgg
     3361 cagaaaaagg tgttaaactt actgctcaga tggttccaaa tgatccaatt tcagactttt
     3421 tgagcttatt aaaataggaa aaaaattttt aggaggtcat tgttatgata caatggtggc
     3481 aaattttact tctcactttg tactcagctt atcaaatctg tgatgagttg acgatcgttt
     3541 catctgcagg ttcccctgta tttgctggtt tcattactgg tttaatcatg ggagatgtga
     3601 ctactggttt acttatcggt ggtaacttgc aactgttcgt tcttggggtt ggtaccttcg
     3661 gtggtgcttc tcgtatcgac gcaacttctg gtgcggttct tgcgacagcc ttctctgttt
     3721 cacaaggaat tgatgcaccg cttgcaatta ctacaatcgc tgtaccagta gcagctctct
     3781 tgacttactt cgacgttctt ggtcgtatga ctactacctt cttcgctcac cgtgtggatg
     3841 ctgcaatcga acgctttgac tataaaggta ttgaacgcaa ctacttgctt ggtgcgattc
     3901 cgtgggctct atctcgtgcc cttccagtct tctttgccct tgcttttggt ggtgcctttg
     3961 tacaatcagt agtagacttt gttgaagcct acaaatgggt tgcagatggc ttgacactcg
     4021 caggacgtat gcttccaggt cttggatttg caatcttgct tcgttacctt ccagttaaac
     4081 gtaaccttca ctaccttgct atgggatttg gtttgacagc tatgttgact gttctttact
     4141 catatgtaac aggtcttggt ggcgctgttg ctggtatcgt aggtactctt cctgctgaag
     4201 ttgctgaaaa aattggtttc gtgaacaact tcaaaggttt gtctatgatt ggtatttcta
     4261 tcgtaggtat tttccttgca gtgcttcact tcaaaaatag ccaaaaagta gctgtagcag
     4321 caccttctac accatcagaa agtggggaaa tcgaagatga cgaattctaa ttacaaactt
     4381 acaaaagaag attttaatca aatcaacaaa cgtagcttgt ttactttcca attaggttgg
     4441 aactacgaac gtatgcaagc ttctggttac ctttacatga tcttgcctca gttgcgtaaa
     4501 atgtatggtg atggaactcc tgaattgaaa gaaatgatga aagttcatac tcaattcttc
     4561 aatacttcac cattcttcca taccattatc gctggttttg accttgccat ggaagaaaaa
     4621 gatggtgtag gttcaaaaga cgctgttaac ggtatcaaga caggtttgat gggaccattc
     4681 gctcctcttg gggatacaat ctttggttca cttgtacctg ctatcatggg gtcagtcgca
     4741 gcaactatgg ctatcgctgg ccaaccttgg gggatcttcc tttggattgc agttgcagta
     4801 gcgtatgaca tcttccgttg gaaacagttg gaatttgctt acaaagaagg ggttaacctt
     4861 atcaacaaca tgcaaagtac cttgacagct ttgattgacg ctgcatctgt acttggtgtc
     4921 ttcatgatgg gtgctcttgt agcaacagtg attaactttg aaatttctta caagttgcca
     4981 atcggtgaaa agatgattga tttccaagac atcttgaacc aaatcttccc acgtttgctt
     5041 ccagcaatct ttactgcctt tatcttctgg ttgcttggta aaaaaggtat gaactctact
     5101 aaagctatcg gtattattat cgtacttgct ttggctcttt ctgcccttgg tcactttgca
     5161 cttggaatgt aattccttat gactaaatca ttaattttgg tgagccatgg tcgcttctgt
     5221 gaggagctta aaggtagcac aggcccacaa gacaacattt acacagtagc tcttcttcca
     5281 gaagatggcc cagaagaatt tactgctaaa tttgaagctg ttattgaagg gttggatgat
     5341 ttcctagtct ttgcggatct tctcggtggg acaccttgta atgtggtgag tcgcttgatc
     5401 atggaaggtc gtgatattga cctttacgca gggatgaatc ttccaatggt gattgaattt
     5461 atcaatgcga gccttacagg cgcagatgcg gactacaaga gccgtgctgc agaaagcatt
     5521 gtgaaagtta atgacctgtt agcggatttt gatgatgatg aagatgaata atactcttcg
     5581 aaaatctctt caaactacgt caacgtcgcc ttgccgtagg tatatgttac tgacttcgtc
     5641 agttctatct gcaacctcaa aacggtgttt tgagctgact tcgtcagtct tatccggcaa
     5701 cctcaaagca gtgctttgag cagcctgcgg ctagtttcct acagatttta gttggaactc
     5761 gattcaattc atgtgacaac gtgaaaatcg ttagagcatt ttatatagaa tatacatggg
     5821 aatggagctt actcccattc ccatatttaa tagaaaaaga ggaactcaat gctacattat
     5881 acaaaagaag acttgctcga attgggtgca gaaatcacta cgcgtgaaat ctaccaacaa
     5941 cctgatgtat ggagagaagc ttttgaattt tatcaagcaa aacgtgaaga aattgcagcc
     6001 ttcctacaag aaatcgctga taaacatgac tatattaagg ttatcttgac aggtgctggg
     6061 acttctgctt atgtgggaga taccttgcta ccttacttta aggaagtcta tgacgaacgc
     6121 aaatggaatt tcaatgccat tgcaacaaca gatatcgttg ccaatccagc aacctatttg
     6181 aaaaaagatg tggcaactgt ccttgtgtct tttgctcgta gtgggaactc gcctgaaagt
     6241 gtggcgactg ttgatttggc caaatccttg gtggatgagc tttatcaagt gacgattact
     6301 tgtgcagcag atggtaaatt ggctcttcaa gctcacggtg atgactgtaa tctcttgctc
     6361 ttgcaaccag ctgcttctaa tgacgctgga tttgccatga cttctagctt tatgtctatg
     6421 atgttgacag ctctcttggt ctttgatcct atagaatttg ctgtgaaatc tgaacgtttt
     6481 gaagttgttt ctagtcttgc ccgtaaagtt ctagacaatg cagaagatgt caaagagctt
     6541 gttgacctag actttaagcg tgtcatctac ctaggcgctg gtcctttctt tgggcttgct
     6601 catgaagctc agctcaagat tttggaatta acagctggtc aagttgcgac tatgtatgaa
     6661 agtccagttg gcttccgtca cggtccaaaa tcacttatca acgaagatac agttgtcttg
     6721 gtctttggta caacgacaga ctacactcgc aagtacgact tagacttggt tcgtgaagtt
     6781 gctggtgacc agattgctcg tcgtgttgtg cttttgagtg atcaagcctt tggtcttgaa
     6841 aatgtcaaag aagtggccct tggttgtggc ggtgtcttga atgatattta ccgtgtcttc
     6901 ccatacatcg tatatgctca actctttgct ctattgactt cactcaaggt agaaaataaa
     6961 ccagatacac cgtctcctac aggtaccgta aaccgtgtgg tacaaggtgt tatcatccac
     7021 gaatatcaaa agtaatattc ttcgaaaatc tctttaaacc atactagtgt cgccttgccg
     7081 taggtatggt tactgacttc gtcagttcta tctacaacct caaaacagtg ctttgagcag
     7141 cctgcggcta gcttcctagt ttgctctttg attttcattg agtataagac agtgtttatg
     7201 aattcttgac aagaggattt gtacatcatc aggcaaacca tagattgtca attcgctttc
     7261 tatggtttgt ttgattgaga gaaaaggtat ctgttaagat aatattcaaa ctacaataag
     7321 gagtaagaaa gaaatgaaga aaattgtatt tgctagcgcc ttggccttga ccttggcagg
     7381 agcagttttg acaaatgatg tttttgcgaa tgacagactg gttgcaacac aatctactga
     7441 tagtaatgta ttgacctcag aggtgctaaa accttctagt ggcaatgttt tggttggaat
     7501 caaaggagaa tttttgcctc ctcatcaaca atctatttta gatgccatta ataaaattcg
     7561 taaagaagca gctgacgaag gtttggtaga taagtatgtt cctgtcaaat ggtcagttga
     7621 ccatgagaaa acggcttttg tacgcgctgc tgaggtatcc gttacgttga aggctgaacg
     7681 tctttccagt aaaaacaact ggactgcatt tccatctggt aatagcctaa gtggagaagc
     7741 cctagatttg aatcctgatg gatttctaaa agccattgag aattggcatg ctgaaaaggc
     7801 gaactatgtg gcgaaaaaga aagataaaac atcaaaagaa ttttcatttt attatgagaa
     7861 cttgattaac cctaaattta cctatgtggg tcttgctgct tttaaaaatg cagctagtcc
     7921 tcagaaggca gcaaccgttg ctttggctct aggaactacg acttcttcag aggaattggc
     7981 tggtggatat ggttctgctg ttcagtacac agaagtgact gcctcaaacc tttcaacagt
     8041 taaaagtaaa gcaatggttg tagaaacacc gttgaaagat ttcagaaaat ctacgtctga
     8101 tcagtctggc tgggtgcagt ctaatggcaa gtggtatttt tatgagtctg gtgatgtgaa
     8161 gacaggctgg ttgaaaacag gtggtaaatg gtactacttg aatgacctag gtgttatgca
     8221 gactggattt gtagaagttg atggatcggt gtattatcta agtaactcag gcgctatgtt
     8281 tacaggctgg ggaacagatg gtagcagatg gttctactgt gatggctcag tagctatgaa
     8341 aacaggctgg tacaaggaaa atggtacatg gtattacctt gatgaagaag ggatcatgaa
     8401 gacgggttgg tttaaagtag gtcaacactg gtactatgca aatggttcag gcgctttggc
     8461 tgttagcaca acaacaccag atggttaccg tgtaaatgct aatggtgaat gggtaagcta
     8521 ggctgaaaag agcaagtcat aggtaaagta ttcatcttac ttagcaaaaa gaatgaaata
     8581 taagaaagag gttgatggca gacattgacc tcttttggtt tataaagatt ggattctttg
     8641 tcgcttcaat ctcagacttt tctattatga gctaatattt tatagtatat taaaagcata
     8701 atcggtaatc taattaaaaa atactgtaat tagtttgaaa ctcctcactt atttgtctag
     8761 attttgtttc cgttttctat tgttgcagtt cattgtattc ttctataaaa aattg
//
LOCUS       DAVKDZ010000079         8810 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000079 DAVKDZ010000000
VERSION     DAVKDZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8810
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.080"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            66..299
                     /gene="secG"
                     /locus_tag="VWZ30_001787"
     CDS             66..299
                     /gene="secG"
                     /locus_tag="VWZ30_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU9335470.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            401..2755
                     /gene="rnr"
                     /locus_tag="VWZ30_001788"
     CDS             401..2755
                     /gene="rnr"
                     /locus_tag="VWZ30_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU9335471.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMCAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKL
                     GRSNDKRKHSQKDEKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            2718..3185
                     /gene="smpB"
                     /locus_tag="VWZ30_001789"
     CDS             2718..3185
                     /gene="smpB"
                     /locus_tag="VWZ30_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU9335472.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            3201..4061
                     /gene="tehB"
                     /locus_tag="VWZ30_001790"
     CDS             3201..4061
                     /gene="tehB"
                     /locus_tag="VWZ30_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEU9335473.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            4134..5087
                     /gene="coiA"
                     /locus_tag="VWZ30_001791"
     CDS             4134..5087
                     /gene="coiA"
                     /locus_tag="VWZ30_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEU9335474.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            5106..6908
                     /gene="pepF"
                     /locus_tag="VWZ30_001792"
     CDS             5106..6908
                     /gene="pepF"
                     /locus_tag="VWZ30_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9335475.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGIYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALVVLGEDYL
                     GRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            6910..7623
                     /locus_tag="VWZ30_001793"
     CDS             6910..7623
                     /locus_tag="VWZ30_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU9335476.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            7690..8631
                     /gene="prsA"
                     /locus_tag="VWZ30_001794"
     CDS             7690..8631
                     /gene="prsA"
                     /locus_tag="VWZ30_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEU9335477.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGSGDSSSSSSTSNE"
BASE COUNT         2837 a         1619 c         1942 g         2412 t
ORIGIN      
        1 taaccctaca tcttgaatct aagtagattt tatggtaaaa taatagggat tttaaggagt
       61 ttgatatgta taacctatta ttaaccattt tattagtatt atctgttgtg attgtgattg
      121 caattttcat gcaaccaacc aaaaaccaat ctagcaatgt atttgatgcc agttcaggtg
      181 atttgtttga acgcagtaaa gctcgcggtt ttgaagctgt aatgcagcgt ttgacaggga
      241 ttttagtctt tttctggcta gccattgcct tagcattgac ggtattatca agtagataag
      301 aaaataatgg gcaggactag gtctttgcct ctttttattt ttaaaggatg tttgagaagg
      361 ttttacagta aaagaaaatt aaaaaatcta gaaagaaaat atgaaagata gaataaaaga
      421 atatttacaa gacaagggaa aggtgactgt taatgatttg gctcaggctt tgggaaaaga
      481 cagttccaag gattttcgtg agttgattaa aaccttgtcc ttaatggaaa gaaagcacca
      541 gattcgcttt gaagaagatg gtagtctgac attagaaatt aagaaaaaac atgagattac
      601 cctcaagggg atttttcatg cccataaaaa tggctttggc tttgttagtc tggaaggcga
      661 ggaggacgac ctttttgtag ggaaaaatga tgtcaactat gctattgatg gtgataccgt
      721 cgaggtagtg attaagaaag tcgctgaccg caataaggga acagcagcag aagccaaaat
      781 tattgatatc ctagaacaca gtttgacaac agttgtcggg caaatcgttc tggatcagga
      841 aaagcctaag tatgctggct atattcgttc aaaaaatcag aaaatcagtc aaccgattta
      901 tgttaagaaa ccagccctaa aattagaagg aacagaagtt ctcaaagtct ttatcgataa
      961 atacccaagc aagaaacatg atttctttgt cgcgagtgtt ctcgatgtgg tgggacactc
     1021 aacggatgtc ggaattgatg ttcttgaggt cttggaatca atggacattg tatccgagtt
     1081 tccagaagct gttgttaagg aagcagaaag tgtgcctgat gctccgtctc aaaaggatat
     1141 ggaaggtcgt ctggatctaa gagatgaaat tacctttacc attgacggtg cggatgccaa
     1201 ggacttggac gatgcagtgc atatcaaggc tctgaaaaat ggtaatctgg agcttggggt
     1261 tcacatcgca gatgtttcct attatgtgac cgagggttct gcccttgaca aggaagccct
     1321 taaccgtgcg acttctgttt acgtgacaga ccgagtggtg ccaatgcttc cagaacgact
     1381 atcaaatggc atctgctctc tcaatcccca agttgaccgc ctgacccagt ctgctattat
     1441 ggagattgat aaacatggtc gtgtggtcaa ctataccatt acacaaacag ttatcaagac
     1501 cagttttcgt atgacctata gcgatgtcaa tgatatccta gctggcgatg aagaaaagag
     1561 aaaagaatat cataaaattg tatcaagtat cgaactcatg gccaagcttc atgaaacttt
     1621 agaaaacatg cgtgtgaaac gtggagctct caattttgat accaatgaag cgaagatttt
     1681 agtggataaa caaggtaagc ctgttgatat cgttcttcgg cagcgtggta ttgccgagcg
     1741 gatgattgag tcttttatgt tgatggctaa tgaaacagtt gccgaacatt tcagcaagtt
     1801 ggatttgcct tttatctatc gaattcacga ggagcctaag gctgaaaagg ttcagaagtt
     1861 tattgattat gcttcgagtt ttggcttgcg catttatggg actgccagtg agattagtca
     1921 ggaggcactt caagacatca tgtgtgctgt tgagggagaa ccttatgcag atgtattgtc
     1981 catgatgctt cttcgctcta tgcagcaggc tcgttattcg gagcacaatc acggccacta
     2041 tggactagct gctgactatt atactcactt taccagtcca attcgtcgtt atccagacct
     2101 tcttgttcac cgtatgattc gggattacgg tcgttctaag gaaatagcag agcattttga
     2161 acaagtgatt ccagagattg cgacccagtc ttccaaccgt gaacgtcgtg ccatagaagc
     2221 tgagcgtgaa gtcgaagcca tgaaaaaggc tgagtatatg gaagaatacg tgggtgaaga
     2281 gtatgatgca gttgtatcaa gtattgtcaa attcggtctc tttgtcgaat tgccaaacac
     2341 agttgaaggc ttgattcaca tcactaatct acctgaattt tatcatttca atgagcgtga
     2401 tttgactctt cgtggagaaa aatcaggtat cactttccga gtgggtcagc agatccgtat
     2461 ccgtgttgaa agagcggata aaatgactgg agagattgat ttttcattcg tacctagtga
     2521 gtttgatgtg attgaaaaag gcttgaaaca gtctagtcgt agtggcagag ggcgtggttc
     2581 aaatcgtcgt tcggataaga aggaagacaa gagaaaatta ggacgctcaa atgataagcg
     2641 taagcattca caaaaagacg agaagaaaaa aggaaagaaa cctttttaca aggaagtagc
     2701 taagaaagga gccaagcatg gcaaagggcg agggaaaggt cgtcgcacaa aataaaaagg
     2761 cacgccacga ctatacaatc gtagatacgc tagaggcagg gatggtcctg actggaactg
     2821 aaatcaagag tgtacgagct gctcgaatta atctcaagga tggctttgct caagtgaaaa
     2881 atggagaagt ttggctgagc aatgttcata tcgcgcctta cgaagagggc aatatctgga
     2941 accaggaacc agaacgtcgt cgtaaactcc tgctccataa aaagcaaatt caaaaattgg
     3001 aacaagagac caaagggaca ggaatgacct tagttcccct taaggtctat ataaaagatg
     3061 gctacgctaa gcttctttta ggacttgcca aagggaagca tgactatgac aaacgggagt
     3121 ctatcaaacg tcgtgagcaa aatcgagata tcgcgcgtgt gatgaaagct gttaatcagc
     3181 gataaaaaga ggaattgaaa atggaaaaat tagttgccta taaacgcatg cctttgtgga
     3241 ataaacaaac aatgcctgaa gctgttcagc aaaagcacaa tacaaaagtt gggacttggg
     3301 ggaaaattac tgtcttgaag ggagctctca agtttattga attgacagaa gaaggggaag
     3361 ttctagctga acacctcttt gaagcagggg cagacaatcc aatggcccaa cctcaagcct
     3421 ggcaccgagt ggaagctgcc acagatgatg tggaatggta cttggaattt tattgtaaac
     3481 ctgaggatta ttttgctaaa aaatacaata ccaatcctgt tcattcagag gtcctagagg
     3541 ccatgcagac agtgaaacaa gggaaagctt tggatttggg ttgtggtcag gggcgtaatt
     3601 ctctttttct agcccagcaa gattttgatg tgacggctgt agatcaaaat ggactagctc
     3661 ttgaaatctt gcaaagcatt gtggagcagg aagatttgga catgcctgtt ggcctttacg
     3721 atatcaattc agctagcatt gaacaagaat atgattttat cgtttcaaca gttgttctca
     3781 tgtttctaca agcggaccgc attccagcta ttattcaaaa tatgcaggag aaaaccagtg
     3841 ttggtggtta caaccttatc gtttgtgcca tggacacgga ggattatcct tgctcggtta
     3901 acttcccatt cacctttaaa gaaggagaac tggcagacta ttacaaggat tgggaattgg
     3961 ttaagtacaa tgaaaatcca ggccatttgc accgtcgcga tgagaatggc aatcgtattc
     4021 aactacgctt tgcgacctta ctagctaaga aaatcaagta aacacacatg aagattagga
     4081 attttcctga tcttttttct tttttacgaa tgatatagaa aaggagggaa ttcatgtttg
     4141 ttgcgagaga tgctagggga gaattggtaa atgtgttaga ggataaactt gagaagcaag
     4201 catacacctg cccagcttgt ggaggccagc tccatttgcg tcaaggacca agtgtacgga
     4261 cgcattttgc ccataaatcc ttaaaagact gtgatttttt ctttgaaaat gaaagtccag
     4321 aacacctggc caataaggaa tccctctatc actggttgaa aaaagagaca aaggttcaat
     4381 tagagtaccc gctttcagaa cttaaacaga ttgcggatgt atttgtaaat ggcaatctag
     4441 ctctagaagt tcagtgtagt cccttgcctc agaaagtcct taaagagcga agtgagggct
     4501 atcgtagtca gggttaccaa gtactgtggt tgctgggtca aaaactgtgg ctcaaggagc
     4561 gtttgactcg tctacagcaa ggttttcttt atttcagtca aaacatgggc ttttatgttt
     4621 gggaattaga caaggaaaaa caagttttaa gactcaaata cctgatttac caggatctcc
     4681 gcggtaaact ccattatcaa atcaaggaat tttcctatgg tcaaggtagt ttattggaaa
     4741 tattgcgtct tccctataag agacaaaaaa tatctcattt tacagtttct gaggacaagg
     4801 acatctgtcg ctatatccgg caacaacttt attatcaaaa tctcttttgg atgaaagaac
     4861 aagcagaagc ctatcaaaag ggagaaaata tcctgactta tggactgaaa gaatggtatc
     4921 cacaaattcg accaatagtg ggcaaatttt tccagattga acaagacttg actagctatt
     4981 atcagcactt ttatacctat taccaaaaaa atcctcaaaa tgattggcaa aagctttatc
     5041 caccagcctt ttatcagcaa tatttcttga aaaatatggt agaatagaaa ggatggagga
     5101 atctaatggt attacaaaga aatgaaataa atgaaaaaga tacatgggat ctatcaacga
     5161 tctacccaac tgaccaggct tgggaagaag ccttaaaaga tttaacagaa caattggaga
     5221 cagtagccca gtatgaaggc catctcttgg atagtgcgga taacctacta gaaatcactg
     5281 aattttctct tgaaatggaa cgccagatgg agaagcttta cgtttatgct catatgaaga
     5341 atgaccagga tacacgtgaa gctaagtatc aagagtacta tgccaaggcc atgacactct
     5401 acagccagtt agaccaagcc ttttcattct atgagcctga atttatggag attagcgaaa
     5461 agcagtatgc tgacttttta gaagctcaac caaagctgca ggtttatcaa cactattttg
     5521 acaagctctt gcaaggcaag gatcacgttc tctcacaacg tgaagaagaa ttattggctg
     5581 gagctggaga aatctttggt tcagcaagtg aaaccttcgc tatcttggac aatgcggata
     5641 ttgtgttccc ttatgtccta gacgatgatg gtaaagaagt tcagctatct catgggattt
     5701 acacacgttt gatggagtct aaaaaacgtg aggttcgccg tggtgcctat caagctcttt
     5761 atgcgactta cgaacaattc caacacacct atgccaaaac cttgcaaacc aatgttaagg
     5821 tgcaaaatta ccgtgctaaa gttcgtaact acaagagtgc tcgtcatgca gccctcgcag
     5881 cgaattttgt tccagaaagt gtttatgaca atttggtagc agcagttcgc aagcatttgc
     5941 cactcttaca tcgctatctt gagcttcgtt caaaaatctt ggggatttca gatctcaaga
     6001 tgtacgatgt ctacacaccg ctttcatctg ttgaatacag ttttacctac caagaagcct
     6061 tgaaaaaagc agaagatgct ttggtagtct tgggtgagga ttacttgggc cgtgttaaac
     6121 gtgccttcag cgagcgttgg attgatgttt acgaaaatca aggcaagcgt tcaggtgcct
     6181 actctggtgg ttcttacgat accaatgcct ttatgcttct caactggcag gacaatctgg
     6241 acaatctctt tactcttgtt catgaaacag gtcacagtat gcattcaagc tatactcgtg
     6301 aaactcagcc ttatgtttac ggagattact ctatcttttt ggctgagatt gcctcaacta
     6361 ccaatgaaaa tatcttgacg gagaaattat tggaagaagt ggaagacgac gcaacacgct
     6421 ttgctattct caataacttc ctagatggtt tccgtggaac agttttccgc caaactcaat
     6481 ttgctgagtt tgaacacgcc attcaccaag cagatcaaaa tggggaggtc ttgacaagcg
     6541 atttcctaaa taaactctac gcagacttga accaagagta ttatggtttg agtaaggaag
     6601 acaatcctga aatccaatac gagtgggctc gcattccaca cttctactat aactactatg
     6661 tatatcaata ttcaactggc tttgcggccg cctcagcctt ggctgaaaaa attgtccatg
     6721 gtagtcaaga agaccgtgac cgctatatcg actacctcaa ggcaggtaag tcggactatc
     6781 cacttaatgt catgagaaaa gctggtgttg atatggagaa ggaagactac ctcaacgatg
     6841 cctttgcagt ctttgaacgc cgtttaaatg agtttgaagc ccttgttgaa aaattaggat
     6901 tggcataaaa tggttgaatc gtatagtaag aatgctaacc ataacatgcg tcgtcctgtc
     6961 gtcaaagaag aaattgtaga cttgatgcgt cagcgtcaaa agcaagtcac aggtttcttg
     7021 aaagaattgg aagattttgc ccgcaaggaa aatattccta ttattcccca tgaaacggtt
     7081 gcttatttcc gttttcttat ggaaaccatg cagcctaaaa atattctgga aattgggacg
     7141 gctatcggtt tttcagctct cttgatggct gaacatgcgc caaatgctaa gattacaact
     7201 attgatcgta atccagaaat gattggtttt gccaaggaaa attttgccca gtttgacagt
     7261 cgcaagcaaa tcactctcct agagggagat gcggtggatg tcttatctac actgacagag
     7321 tcttatgatt tcgtctttat ggattctgcc aagtctaaat acatcgtctt tctgccagaa
     7381 atcctcaaac atttggaagt tggtggtgtg gttgtcttgg atgatatttt tcaaggtggt
     7441 gatgttgcca aggatattat ggaagtccgt cgtggtcagc gaaccattta tcgaggcctt
     7501 caaaaattat ttgatgcaac cttagacaat ccagaactca ccgcaacatt agtgccttta
     7561 ggagatggta ttctcatgct tcgtaaaaat gtagcagatg ttcaactgtc tgaaagcgaa
     7621 tgattttcag aaaaatttaa gaaaaaatag taaaatagat agagtaacac ttatctcaaa
     7681 ggagtagaca tgaagaaaaa attattggca ggtgccatca cactattatc agtagcaact
     7741 ttagcagctt gttcgaaagg gtcagaaggg gcagacctta tcagcatgaa aggggatgtc
     7801 atcacagaac atcaatttta tgagcaagtg aaaaacaacc cttcagccca acaagtcttg
     7861 ttaaatatga ccatccaaaa agtttttgaa aaacaatatg gctcagagct tgatgataaa
     7921 gaggttgatg atactattgc cgaagaaaaa aaacaatatg gcgaaaacta ccaacgtgtc
     7981 ttgtcacaag caggtatgac tcttgaaaca cgtaaagctc aaattcgtac aagtaaatta
     8041 gttgagttgg cagttaagaa ggtagcagaa gctgaattga cagatgaagc ctataagaaa
     8101 gcctttgatg agtacactcc agatgtaacg gctcaaatca tccgtcttaa taatgaagat
     8161 aaggccaaag aagttctcga aaaagccaag gcagaaggtg ctgattttgc tcaattagcc
     8221 aaagataatt caactgatga aaaaacaaaa gaaaatggtg gagaaattac ctttgattct
     8281 gcttcaacag aagtacctga gcaagtcaaa aaagccgctt tcgctttaga tgtggatggt
     8341 gtttctgatg tgattacagc aactggcaca caagcctaca gtagccaata ttacattgta
     8401 aaactcacta agaaaacaga aaaatcatct aatattgatg actacaaaga aaaattaaaa
     8461 actgttatct tgactcaaaa acaaaatgat tcaacatttg ttcaaagcat tatcggaaaa
     8521 gaattgcaag cagccaatat caaggttaag gaccaagcct tccaaaatat ctttacccaa
     8581 tatatcggta gtggagattc aagctcaagc agtagtacat caaacgaata gtccaaatca
     8641 atgagtcagg gaaaaaactc gacttcagga aaaaatgaag caaacattcc cacaataaaa
     8701 cgcatagtac aaggtttgta ctgcccccca aaagttagac aattaattta tccgaaggac
     8761 ttagttctgt attgcacagg gctaagtcct tttagtttta ccttaattct
//
LOCUS       DAVKDZ010000080         8789 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000080 DAVKDZ010000000
VERSION     DAVKDZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.081"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..144
                     /locus_tag="VWZ30_001795"
     CDS             <1..144
                     /locus_tag="VWZ30_001795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335478.1"
                     /translation="KHCFEVVDETDEVSSKHCFEVVDETDEVSNHTYGKATLTWFEEI
                     FEE"
     gene            complement(196..678)
                     /gene="greA"
                     /locus_tag="VWZ30_001796"
     CDS             complement(196..678)
                     /gene="greA"
                     /locus_tag="VWZ30_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU9335479.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVIIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVDSYDVKILKVEKTA
                     "
     gene            complement(749..2404)
                     /gene="mltG"
                     /locus_tag="VWZ30_001797"
     CDS             complement(749..2404)
                     /gene="mltG"
                     /locus_tag="VWZ30_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU9335480.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPRPATETVDI
                     ISDTSRRSRREGAKPVKPKKEKKSHVKAFVTSFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAETF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRNR
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(2485..2979)
                     /locus_tag="VWZ30_001798"
     CDS             complement(2485..2979)
                     /locus_tag="VWZ30_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9335481.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(2976..3440)
                     /locus_tag="VWZ30_001799"
     CDS             complement(2976..3440)
                     /locus_tag="VWZ30_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9335482.1"
                     /translation="MEIPIKIIQASKSDLPEIEALQTSSFPAEKQQPSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEDATLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(3450..4784)
                     /locus_tag="VWZ30_001800"
     CDS             complement(3450..4784)
                     /locus_tag="VWZ30_001800"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU9335483.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADKNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLKDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFESEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFVHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(4796..5413)
                     /locus_tag="VWZ30_001801"
     CDS             complement(4796..5413)
                     /locus_tag="VWZ30_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9335484.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(5461..8559)
                     /locus_tag="VWZ30_001802"
     CDS             complement(5461..8559)
                     /locus_tag="VWZ30_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9335485.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSGCAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPTLFMEDYQ
                     GVSGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(8652..>8789)
                     /locus_tag="VWZ30_001803"
     CDS             complement(8652..>8789)
                     /locus_tag="VWZ30_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335486.1"
                     /translation="EAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAK
                     C"
BASE COUNT         2407 a         1995 c         1686 g         2701 t
ORIGIN      
        1 aaacactgtt ttgaggttgt agatgaaact gacgaagtca gctcaaaaca ttgttttgag
       61 gttgtagatg aaactgacga agtcagtaac catacatacg gtaaggcgac gctgacgtgg
      121 tttgaagaga ttttcgaaga gtagcaaaac agaaaaagga gtgagtgaag cgcatcgcca
      181 ctcctttttc tgtttttagg ctgttttttc aaccttcaag atttttacat catagctatc
      241 aacaggcgtt tcaatggttg ctgtgtcacc tgttttcttg ccaatcaagg cctgcccaat
      301 tgggctttca tttgaaacct tacctgcaaa ggcatccgca ccagctgaac ctacgataat
      361 ataaacttct tcttcgtcct caccaatttc ttggatgatg actgttttac caatcgctac
      421 ttcgtcctgg gcaactgcgt cgctattgac gatttcagca tagcggattt ttgtttctaa
      481 gctagagatt tgtccttcga caaaggcttg ttcatcctta gctgcttcgt actcactgtt
      541 ttctgaaagg tcaccgtatg aacgggcaat cttaatgcgt tctaccactt ctggtcgacg
      601 aaccaatttc aattcttcta attctttttc aagtttttcc ttttcctcaa gggtcatagg
      661 atatgttttt tctgccattt ttctcaactt tcttctgata atattttcta aagaaaatta
      721 tgtgaagtat cacataattt tagtttgttt agtttaattt gctgttgaca tgttcagcga
      781 cattgcggtc gtggtcttct tgattgttag catagtagac cttgccttct gtgacatctg
      841 ctacaaagta gaggttatcg ctcttagttt gattgatgct tgactcaatc gcatccagac
      901 ttggactatc gactggacca ggcatgagac ctacattttt ataaacatta taaggtgaat
      961 caatgttggt atcaatcgca acatcctcag ctagactgat attttgcccc agttttcctt
     1021 gggcatacaa gattgcaata ttactttgaa gtggcctatt acgattcaaa cgattgtaga
     1081 atacacctgc aatgagctta cgatcttctg tcttggcacc ttctttttcg accaaggaag
     1141 caatggtcaa caactcattg acagtcaagt ttttagattt gatagtacta tagtaaggag
     1201 ataggttctt atccatagca gctaacatct catcaatcaa gctctcaata gttgtgcttt
     1261 ccttgataga gtatgtagct gggaaaaggt atccttccaa acgataacgc gcaccgctgt
     1321 cttttacagg caaactttcc agtaaagtag gatatttagc tactgcttga ctgataaacg
     1381 tctcatcttg aactttagcc aagaaagtct ccgctgtcaa agactctttg aagtcacctt
     1441 gcaattgacc cacagcttga gcaatctgat ccaaggtata accttctgga attgtcaaag
     1501 tcgcaagtac aggttcttgc ggttcatctg ttccaccttt ttgcaactct ttgagtaagt
     1561 cttctgtact catactcttt tgcaaattgt agtaacctgc tttcaagtcg gtataatttt
     1621 tatacttggc ataaaaacta aaaatcagac catgctttac caaaccagct ttttcaagcg
     1681 tcgtaccgat ttcttgaacg tttgaacctt ctggaattcc aaccgtcaca tatttcttag
     1741 aattagcatc gataggtaac aaagaatcta gcacgtactg gtaaccaaag taaccacctg
     1801 ctgagagcaa ggcaaggaat acaaggaatg aagtcacaaa agctttcaca tgtgacttct
     1861 tctctttctt aggcttaacg ggttttgctc cttctctacg gctacgacgt gatgtatcgc
     1921 tgatgatatc cactgtttct gtagcaggcc taggtgtttc tggacctgct tgttctgctt
     1981 ttttctctgc cgtcgtatag gaaacagcta cccttgttgg ggtttcattg tattctcttt
     2041 caagtttctt aggtctaaca ggacctggac ctggtcttga tccactttct tccgctggag
     2101 aagaaggtac atcttgactt ggatgacttg gaacaccagg agtttctctt tgaatctcat
     2161 ctgctggaga agctggtaca ccttgacttg ggtgagtagg cacggtagga gcttttctca
     2221 taatctcctc taccgttgac aaggaatcag ccatgagttc ctcagctgaa ggttcatttg
     2281 caggagtgcg aactactgcc tcatcttctt tcagaacttc atcatagcct tttacttttt
     2341 ctaaatctct cagaatctgc tctttaaagc ttaatttctc ttcttctctt gacttttcac
     2401 tcaaaagttt ttcctccttg ttgataatcc ataatattat atcttaaaag tcaaaaaaaa
     2461 gcaatctatg atacttttcc taacttattt tttcatttcc cagaccatat cataccatgt
     2521 ttcccctgca aaggttgact gggactggcc ttcgttgaca aatccatgtt tttcataata
     2581 ggcgatgaga tagtcatgac aggttagatt aatgccatct ctctcatctt caagagcaac
     2641 ttctttcaag gctgtcagca atttctgacc cagtccgagt ccctgtgcct ccttggcaat
     2701 agacagacag gtcacagaga tataaccacc aggctcatga ctatagtctt ttatttcttc
     2761 tgtaaaagac tggtcttgca gatggcggtg caggccaact ggtccttcga tataacccat
     2821 gattcttcct tctttttcag caaccagaaa agaggtctga atttctctca aatgtgcttc
     2881 aaagacagaa ggaggaatgg cttcttcgac cgaaaaattt tcaaattcaa gttcaacaat
     2941 ccgatccaaa tcttctaatc ttgcttgtct gattttcatt gttcctccag ataaaaggga
     3001 ttaaaccaaa tcatactata gccctggcta gttgcataga gcaaagttgc gtcttcatca
     3061 acaaaaccgt tcatttcaaa ataggaaagc agctcatcag gactctccaa acgaatccct
     3121 ttgtaatcca gctcaactgc cacctctttc aaggctgcaa gaagaagtgt tcccaagccc
     3181 tgtctctgat ggtcagactc gatgactaaa gaatgtactt ttagatattg cggattgtct
     3241 gactggggac ttgataaaat atagcctaaa agttgatttt catccctagc tagaagaaag
     3301 gtatccgcac acttacggat actttcttct aaaatatggg aaggttgctg cttttcagct
     3361 ggaaaagacg aggtctgaag tgcctctatc tcaggcaaat cagacttgct tgcctgaatg
     3421 atcttaattg gaatttccat gggaacatcc tattgaacat tgcttgtcaa gttagacaag
     3481 agacgctcaa atgagtattc ataggtttgg atgtctcctg ctcccataaa gacgtaaaca
     3541 gcattgtcat ggtctaggag tggagaaaca ttttcaacag taatcacttg gtgttttttg
     3601 ttgattttgt tggctaggtc ttctacctta acgtcaccat gatctacttc acgagccgag
     3661 ccataaattt gcgctagata aacagcatct gcttggttta aagcatggac aaagtcgtcc
     3721 aacaaggcaa tggttcttgt aaaggtatgc ggttgaaaga ctgctacaat ttccttgctt
     3781 gggtatttct gacgagccgc atccaaggtc gcaataattt ctgttggatg gtgggcaaag
     3841 tcatcgataa tcactgtatc attgacaatt ttctcagtga aacgacgttt aacaccggca
     3901 aatgttttca agtgctcacg caccaagttc aaatcaaatc ctgctgtgta aagaagacca
     3961 ataacggctg tcgcattcat gatattgtga cgaccaaagg ttggaatgtg gaattgcccc
     4021 aagttttgtc cacggaaatg aacagtgaaa gttgaaccag ttgttgaacg aagaagatca
     4081 ctagctacaa agtcattgcc ttcagattca aaaccataat aataaattgg tgcatcagac
     4141 gtaatcttac gcaattcagc atcttcacca tagacaaaaa gacccttggt aatttgtttg
     4201 gcatagtcgt taaaggcatt gaaaacatcc ttgagacttg tgaaataatc tggatggtca
     4261 aagtcaatgt tggtgataat agagtattct gggtggtaag gcatgaagtg acgctcatat
     4321 tcatcagatt caaagacaaa atatttggca ttggccgaac cacgacctgt cccatctcca
     4381 atcaagaagc tggtatctgt aatgtgagac aagacatgag acaacatacc tgtcgttgaa
     4441 gtttttccat gtgctcctgc tactcccatg ctaacaaagt cacgcataaa gctacctaga
     4501 aactcatggt aacgtttgta gctgatacca tttttgtccg cataggcaat ttcgacgttg
     4561 ttatctggac gaaaggcatt tccagcgata atttccatat caccgtctag attcttttca
     4621 tcaaaaggaa gaatggtaat tcctgcctgc tcaagaccgc gttgggtaaa gtagtacttt
     4681 tcaacatctg atccctgaac cttgtgcccc atctggtgca acatcaaggc caaggcactc
     4741 atccctgatc ccttaattcc gataaaatga tatgtctttg acatgttttc tccccctatt
     4801 ctgtcattct ggtcagattc aactcttggg caacccgacg ttcttgttct gtttgtttac
     4861 tttttttatt gtagatttgg ctcttcttta gaaaatcata attgtttttc tttggagcag
     4921 gtgctgacac ttcttcattc ttggtaggga tagaatgaac ttcttccgcc aagatataat
     4981 gagactgggt caatttttgg ctatatttga caaattcacc aggattttcc ttttggaaag
     5041 gagttgtcgg ttgattgccc tgtctaacta gactgggctg agaatgacgt ctcgcaaggc
     5101 tgaaatcctg agttaggtag ttagcagagc gtttcttttt caagtccgca cgcgcttctt
     5161 cacgcgccac ctccgcatag ctctttcctt cttttttaac ccctaaagga gcctttttag
     5221 gtttctcgac ttgcttttca atcggtttta ctggtttttc ttcagcaata ggagcccatt
     5281 ctaaataatt tttatctcga tactcaccct tgatattact gatcagatca gactcatcat
     5341 agagattcat gactggcatt tcagtcaaca tgacctcgtc atctgacacc aatggaaatc
     5401 gttcttgttt cattttctat ttcctttcaa cacttcatta tagcgtattg tcttgatttt
     5461 tcaagtgctg gcttcagaaa ttcccaaaat ttctctaatt tctgctaggg tcagactacc
     5521 acgtgactct gtgccgtcca atacttgtga caccagatgt ttcttttgtt cttggagttc
     5581 ctgaattttt tcttcaatgg ttcccttggt caccaagcga tagacctcaa ccgtttcttc
     5641 ctgacccatc cgatgggcac ggccaatggc ttgcgcttcc accgcaggat tccaccaaag
     5701 gtcaaccaag atcactgtat ctgcacctgt caggttcaga ccgaccccac cagccttgag
     5761 ggaaatcaga aaggcatctc tttctccttg attaaaggcc ttggtcatgt cttgtctttc
     5821 cttggctggg gttgaacccg taattttaaa ggaagtcagg cccaagtctg gcagttcttg
     5881 ttcaattttt tccaacattc ccttgaactg agagaaaatc aagacacggt gtccgccgtc
     5941 tgccacctgt accagtaggt ctcggagact atctagtttg ccactgactc cctgataatc
     6001 ttccataaac agggtaggag tgtcacatat ttgacgcaag cgcatcaaac cagataaaat
     6061 ttccacacga cttcgctgaa attcctgttc tgacacttga gccagatggt ctcgcatctg
     6121 ttgtaactgg gcaaggtaaa tagccttttg ctggtcttcc agttcatttt tataaaccac
     6181 ctcaatcaag tctggcaatt cagtcagaac ttcttctttc ttgcgtcgca tcacgaaagg
     6241 cttgataaac tgagccactc gctctgctgg caatttcata aattctttct tgcttggcaa
     6301 aagtccgggc atgacgattt ggaaaataga ccacagctca cccagatggt tttcaatcgg
     6361 agttcctgac aaggcaaaga ccgacggcac cacaaattgt ctcaaggtct gggcaatctt
     6421 ggtctgggca tttttcatga cctgagcctc atctaagaaa aggaagtcaa aggccatccc
     6481 ctgataaaac tcactgtcct gacggaaggt ggcatagcta gtcacataga tttgatggct
     6541 ctcggcaaga atctcctcac gacttgcttt caaaccatga acaacagcca catccaactg
     6601 tggagcaaat ttctgaaact catctgccca gttgtaaatc aaacctgacg gagcgagaat
     6661 caaaacccga ctttcttttg tcacttgact agtcaaaaaa gcaatggtct gaagggtttt
     6721 cccaagtccc atatcatcag ccaaaatccc accaaaacca taatgatgga gcatctgcaa
     6781 ccagccaatt cccttttcct gataatctcg caagtcagcc tgaacctgag ttgcctgtcg
     6841 aggaaagtcc tctggatgtg tcaaatcctg ggccagattc tggaattctt gtgaaaaaga
     6901 aacacggtct cgcccttcaa agagatgagc taaactgtag gccaaggatt tcctagcctg
     6961 caaagtccca tcttttaatt caaattgccc cagttcctgt aaattttgac gaattttctt
     7021 ggtttcttca tcgaaaaagt aaacttgatt agacgaatca atataaaaat cctgattggc
     7081 aaccaaggcc tgcatggctt ggtcgatttc ctcctgggca atatcttgaa aatcaaactg
     7141 gatttccaag agacctccct tggaggcaat ctgcacctga ggactcgcta ggctataaag
     7201 ctcttctagt ttatctgata ggtcaacatg cccgagtttt tcaaagactg gaatgatatc
     7261 atgaaaaaaa tgatagacag actccgcttt taaggcctga cgccaagatt gaaaatcggc
     7321 ttcaaagccc gcagccaaac agacttggaa aattctttct tctaagtccg cgtcacttga
     7381 aaatggtaat tcttctagct cttgtcggct agatacctgt ctatttccat aatcaaactg
     7441 aatttctaaa cgaatccgat tatcttcttc cctgtcaaag taaaaagagg gcgcaaaagt
     7501 tttgatttgt agacgttctg gagctgaaac ggtgcccatc tggataaaaa gagtcagaca
     7561 ggaggccaat ttgtctcgat cactgctatc aaattgcagg tatttctttc cttgttgacc
     7621 cacaggtaac gctttaattt ccttgagaag acgcatctgc tggtctgtta aaaaataaac
     7681 ctgaccttta tggaaaagta ctgctccttg ataaaagaca ttgaccctag gactctcact
     7741 gatttccatt tcaaaatagt ccgagtattc tgttactgta aaggcaaata gattggcatc
     7801 agcatgcata tcctgaaaaa gcagggtttg gtagctatcc acttgatggt caaattgaaa
     7861 atggggcaag gccatcagta aattcacacc ctgctcaaaa aaggtcagag ggaaaaagag
     7921 gtgccgacct tggttttgga aaaagaggtc tggaaccagc ccttcctccg ttagtccgtg
     7981 caagaaagtc aaaagttctt ggctggcatc atcaaaggct tcccaagaaa gagactcctc
     8041 ataaatcttg ccaatcatat acgactttct ctgctcgaca atccttaaaa aaagtggaat
     8101 atcccgaatg acatagtatt tttggctatt gatttggccg attctcagag tccacaagat
     8161 atgattggtt cctgcttcca cctgacccac agctgataac tcataggcgc atcctgattt
     8221 tggagataaa attcgatcca aaaacttgcc acccaaggtc accttggttt caacggcctc
     8281 tttttcttca tgaccttctt ccagactcca caagatttcc tgaccacgct catcattttt
     8341 tagaaaatgc tctagcgctg ccaaatgcac acagtagccc ctcttttgaa aaaaatcaca
     8401 ggcacaaaaa accaaatcat cctctaaact atagcgcagt tcttcttctg caacgcgagc
     8461 gtagagtcga ttgttctttt ccttgatgat atcaacctta ccagtttcat aaagggcaac
     8521 accttcgata cgaattttcc ccggaatcaa tttagccata ttttcacctt taccttatct
     8581 gtttattata ccatattttc gcctatgaaa atagccttct aggaagactt ttctcctaga
     8641 aggctggatt tttaacattt ggcaaaagta gccacaatcc gctgacagac ttcttgcaac
     8701 agagatttgg gcatagctat attgatgcgg gcatggagac ttccttcctc tccaaaatcc
     8761 aaaccacggt tgaggataac cttggcttc
//
LOCUS       DAVKDZ010000081         8597 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000081 DAVKDZ010000000
VERSION     DAVKDZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8597
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.082"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..8223
                     /locus_tag="VWZ30_001804"
     CDS             <1..8223
                     /locus_tag="VWZ30_001804"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEU9335487.1"
                     /translation="ITPTTASDIVKVDSDSNADGDPGTSDEGEPKQALTDKPAELKAA
                     DEVKTQEAPAEGANQEQAGDESETTGVESNSAQPETNPASQEAPQAEGEGKQDQSTPV
                     SDQTSSTSLLQPRVLKSKQSDYDSIPLGDDEDDEDDVRDGLFSSENPVTTIQPRSSRS
                     VQPKENSSGVHSVRAEAKQSRFGQVKGKPLIHGDNPANYIKFKNANGQEVQKPAGVDV
                     AWAEKPSTTQEGLNKTGRIKITYHLTNENGMEVNREEFVTISTPVYHATLTQNRYVTT
                     YGGEFTNRINPTDGRRYINYNAKSHFQLNNLRAYWEHSSGSNGSTYSSVIRDWNTRYL
                     GKKREKLMVRYPSDDGIYRSNRDDGGERYEILDGTFIVKPVKPSIQTSLGKVGKNTLT
                     VNNVNSGTTVVAYDMANPRIPREIGRVSVPKDGDYRIKNGIELTLNSGEVLRKDQKIA
                     TKVIYEITNADQRTESDSSDTLTVKESLVANGIHVIKDETYTGNVKDRIRYNDDTDAD
                     RRTAFPNNASASWVQNPDYSRLGTRNYSANVRIPGQGDARVDVPVHVYAPASLKASSY
                     NNKQGTLSNGTEAENYIQFRDGNATIDKPNNVTVRWKDGQAPDVSRPGTQQKTIEVVY
                     PGNDGPSSTVIREYQVTFTSYHAQAQKREYTRTIGENFTSTTAKSYVKKADGSPELPQ
                     ETEYAWKKDETANREYGSETWGKVNDDWLGKKTNKIKVYYPNADGGNNKADNLAEETE
                     EITFITKPAKPRIASNLTGQAGTRSNVVIQNATPGTTLELRDGDTVLGKVEVPKNNSL
                     YSQLTTATITPTADIPASANITVKSIYSPNNQDQRVESDSSDAVASTQITVSAKGTIQ
                     TLAGTGHIAGLSNLNKATLSTLLNLSDGSAVADGTTGRWESGQNIAKGQAGTRTEKLF
                     VRLPGHTKEQEVTFTVKTLAVPSAKAVVKDKGQDIVADDLSNYVTVEGQTGLSWKGNP
                     SKVEVGKTLPKIKVTYPRAGQNGIAVTDIQDQEVDAKVYSLEVNGVAKTRVTVGETFD
                     PVAADYVTQVANTETLPSGVSYAWKNGNKPSSARVGKETYTVETSFGIGNDVPAELRG
                     QKVETQVEVTVLSTKPSQPELGQNRNDLAITAVVGKENANKAVITFRDDADQEQTVTF
                     EKGANNQWDKVGGINQPTVQILNNNDGTAIVHLTAGTAKVGSSVSIKQQKADSDYSEA
                     ATLVAKERLDGVSATSKDDGSVDVVVPSEAKKASVTYTPEGQSQAKTVELAKAQDGTW
                     SAPANSGLLVNKDNTTGILTITVPASQVVDGSKVVGNADSDSKLSIDAEARAKAPQPV
                     EFDSSIRHNGDIVLTLPNNADTATINYPVSDTELKTATATKGQDGSWRLSPDSGLELA
                     STNGVTKITLAYTKLSGNRTVTASAKAGSGEGESKERTFTKTVPAHTTPTTQNVVIAA
                     NATPTDDQLLTGVIANNKQSVAAKQTQTAIAAGTTKEIPATLTYTDGSTEEITITVQS
                     KPTAPTVNGLESRARVAVPGLLSTARTITGQAMQGAEKVKLTLQNGSEKEVDVAADGS
                     WSYTLAADEFLTQTISNSNAKYSSTKIRLVQVKDGLESEPKDIDILMGRATIDTPLRA
                     GRDITLHIPHDTTSGYIRIGGSVERGGVDIGLKKVNGVWTLDTDANRASKLELVSETD
                     PTNPALTKVTLKVKATDNASYSPPFTIGGDSGNVKFRAHYYSGRDIGAVVPMGRQGQF
                     EWILSGQPTNTRPTVGWETGKEIQDGQKIPSPTVDELKDFFKGEDAEDDAGLTVGYSA
                     SNRGKLRVRLYTSGTNQTVRTNVRGRIDPGNYRLLLSTIDAAGVESNILERNVIIQSY
                     ADYYRDAVQYPTTAQKVTYSDTDITNGNFTAAAKTRFKDKIETINRQNTQLPTSVTYS
                     VGTTDDKAKVAVINFPDGSTIDISHSQVAKPDVPTITPTDTEQQGMPKVTDADREISG
                     TALQNATKVTLKLQTGKGIEIVKEITVNNTKDFAALVPGEGLLKNGVWKYKLADGQYL
                     RQTDATAEPGSSSLPLKATQTVFDAVSDDTSIYVANKRTVEGKKIEGEVGSPDLKKYI
                     DKPQDAVVYKEKGQEKPFPSDFIAKWENTPNFDTVGTFTYKVKFYEKDGNTPADHISE
                     GVEVTFVVKSKAPAILDYTNRDNGETLVNIPQDADEVVFSILRSDTAVDTITVKKSEG
                     WTATGIQKRDNTWVFPANSVHGNRTVTAVATAGKGDTKSVEMLTLITTLAHDVTVNEI
                     TKPTKGNPTSQDLLDAVEVTNKKSVALKQGENYPETFGTHQIHLIVTYQDDSTEEKVA
                     NYTVPDTRDAAKDDIDTKATAKKNDIEADDKLTTEEKQAAKDKVDAAAQSAKDAVTAA
                     TTNDGVTQAQNDGTTAVNNVDTTPKAKPSAKAEIDAKVQDANNAIDQNQDLTDDERRE
                     AKKAVKDAADEAKANIDKATSAGELTTAKNNGLDKLNEPQDASVKQKAKDDIDTKATA
                     KKNDIEADDKLTTEEKQAAKDKVDAAAQSGTNPVTPPSTTRSRRTPPRRSRRSVGFVG
                     NSQTGTTPSAVDKSELQSLVEDLERRLQDLADLSPEALEEAQSILREAQVALANDSLT
                     AQELTELLAKVRQALNSIQAGTSADKSPSTSNSNKEAESAKEPTNATDVPLYGVFGAA
                     VLSLLGALLFAVARKKNSQLDKLSRELDQLLVELEASDKDKKGLGKAKKLAKKARTFV
                     DSQQKDPQKEAELISEIKTILSQLREGV"
     gene            complement(8372..>8597)
                     /locus_tag="VWZ30_001805"
     CDS             complement(8372..>8597)
                     /locus_tag="VWZ30_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9335488.1"
                     /translation="IFQTVFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLIKRNAF
                     GFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT         2928 a         1682 c         1999 g         1988 t
ORIGIN      
        1 attactccaa ctacggcaag tgatatcgtt aaggtagact ctgatagcaa cgcagatggg
       61 gatccaggaa cctcggatga gggagagccc aagcaagctc tgactgacaa gccagctgaa
      121 ctcaaagcag ctgatgaggt gaaaacgcaa gaagcgccag ccgagggagc caatcaagag
      181 caagcaggag atgagtctga aacgactgga gtagaatcca actctgctca gccagagaca
      241 aatccagcta gtcaagaggc tcctcaagca gagggagaag gaaaacaaga ccagtcgact
      301 cctgtttccg accaaacaag cagcaccagc ctcctccagc cgcgggtgct taagagcaag
      361 cagtctgact atgactctat tccacttggc gatgatgagg atgatgagga tgatgtaaga
      421 gatgggctgt tttcttcgga aaatccagta accactatcc aaccacggag ctctcgctca
      481 gttcagccta aagaaaatag ttctggagtc cattcagtaa gagcagaagc caagcaaagt
      541 cgattcggac aggtcaaagg taaacccttg atacatggcg ataatccagc taactatatc
      601 aagtttaaaa acgctaatgg acaagaggtt caaaaaccag ctggagtaga tgttgcttgg
      661 gcggaaaaac caagtactac acaggaagga cttaataaaa caggtcgtat caaaatcacc
      721 taccacctaa cgaatgaaaa tggtatggag gtaaatcgag aagaatttgt aactattagc
      781 acccctgtct atcatgcgac tttaacacag aaccgctatg taacaaccta cggtggggag
      841 tttaccaatc gtataaatcc tacggatggt agacgctata ttaactataa tgcaaagtct
      901 cattttcaac taaataactt gcgtgcttac tgggaacatt cttctggttc aaatggctca
      961 acctattcct ctgttattcg agattggaat actcgttatt taggtaagaa acgtgagaaa
     1021 ttgatggttc gctatccaag tgatgatggc atctatcgta gtaatagaga cgacggcggt
     1081 gaacgatatg aaatactaga tggaactttc atcgttaagc cagtcaaacc aagcattcaa
     1141 accagtcttg gcaaggtagg aaaaaatacc ctgactgtca ataatgtcaa tagtggaaca
     1201 acagttgttg cttacgacat ggcaaatcct aggattcctc gtgaaattgg acgtgtatca
     1261 gttccaaaag acggggatta caggattaaa aatgggattg agcttacgct taactctggt
     1321 gaggtcttac gaaaagatca aaaaattgcg actaaagtta tctatgagat tacaaacgct
     1381 gaccaacgca cagagtcaga tagtagtgat accctgaccg tcaaggaaag tttggtagct
     1441 aatggaattc atgtcatcaa ggatgaaact tacacaggca atgtcaaaga ccgcattcgt
     1501 tacaatgacg atacggacgc tgatcgccgc acagcgttcc ctaataatgc aagtgcgagc
     1561 tgggtacaaa atccagacta tagtagatta ggtacacgta actacagtgc caatgtaagg
     1621 attccaggtc aaggagacgc tagggttgat gtaccagttc atgtctatgc gccagcgagt
     1681 ctgaaagcaa gctcttacaa taacaaacaa ggtactctat ctaatggtac agaagcagaa
     1741 aactatatcc aatttagaga tggcaatgca acgattgata aaccaaataa tgtcactgtg
     1801 cgttggaaag atggacaagc tccagatgtt tcacgaccag gaactcaaca aaaaacaata
     1861 gaagttgttt acccaggcaa tgacggacct agctcaacag tcatcagaga gtatcaagtg
     1921 acgtttacta gctatcatgc acaagctcag aaaagggaat atactcgtac aattggggaa
     1981 aactttacct caaccacagc taagagctat gtcaaaaagg cagatggttc gcctgagttg
     2041 ccgcaagaaa cagagtacgc ttggaagaag gatgagacag ctaatagaga gtatggttcg
     2101 gaaacatggg gcaaggtgaa tgatgactgg ctcggtaaga agaccaataa gattaaagtt
     2161 tactatccaa atgcggatgg aggcaataac aaggcagata atctagctga agaaaccgaa
     2221 gaaattacct ttattacgaa accagctaaa ccaagaattg caagcaattt gacaggtcaa
     2281 gcaggcacac gttcaaacgt agtgattcag aatgcgacac caggaacaac gcttgaattg
     2341 cgtgatggtg atactgtttt aggtaaggtt gaagttccga aaaacaatag tttatacagt
     2401 caattgacaa ccgcaactat tacgcctacg gctgatatcc ctgcttcagc gaatatcact
     2461 gtcaagtcta tctattcacc aaataatcag gatcaacgtg tggagtctga cagtagcgat
     2521 gcagtagcaa gtacccagat tactgtgtct gcaaaaggaa ccattcaaac cttggcagga
     2581 acgggtcata ttgctggttt gagcaacctc aataaggcaa cgctttctac cttactcaat
     2641 ctttcagacg gaagtgcggt cgctgatggc acaacaggac gttgggaatc tggtcaaaac
     2701 attgccaaag gtcaggcagg cacacgaact gaaaaactct ttgttcgctt gcctggtcat
     2761 acaaaagagc aagaagtaac ttttacagtc aagacgctag ctgttccgag cgctaaagca
     2821 gttgtgaaag acaaaggaca agatatcgtt gcagacgact tgagcaacta tgttacagta
     2881 gaaggacaaa ctggactcag ttggaaagga aatccaagta aagtagaggt tgggaaaact
     2941 cttccaaaaa tcaaggtaac ctatccaaga gctggtcaaa acgggatagc cgtgacagat
     3001 attcaggatc aagaggtaga tgctaaggta tacagcctag aagttaatgg tgttgccaag
     3061 acaagagtaa ctgtaggtga aacatttgat ccagttgctg ctgactacgt cacacaggta
     3121 gccaatacag aaactcttcc atcaggtgtt agctatgcat ggaagaatgg caataagcca
     3181 agttcggcta gagtagggaa agagacctac acggtagaga caagcttcgg tataggaaat
     3241 gatgtccctg cagaacttcg tggtcaaaaa gttgaaacgc aggtcgaagt gacagtcctt
     3301 tctactaaac caagtcagcc agaacttggt caaaaccgta atgatttagc aatcacagcg
     3361 gttgtcggaa aagaaaatgc caataaggca gtaatcacgt ttagagatga tgctgatcag
     3421 gaacagactg tgacctttga aaaaggggct aacaatcaat gggataaggt tggaggaatt
     3481 aatcaaccga cagttcaaat tctaaataat aatgatggaa cagctatagt tcacttgaca
     3541 gcaggtacag ctaaagtcgg tagttctgta agcatcaaac agcaaaaagc agattcggac
     3601 tacagtgaag ctgcaacttt agtagctaaa gaacgtttgg atggtgtcag cgcgacaagt
     3661 aaagacgatg gttcggtaga tgtcgttgta ccaagtgaag ccaagaaggc ttctgtgacg
     3721 tatactccag aaggtcaaag tcaagcgaag acagttgagt tagccaaggc tcaagacggc
     3781 acatggtcag cgccggcgaa ttcaggtctt ctagttaata aagacaacac aacaggcatt
     3841 ctgaccatta cggttccagc aagccaagta gtagacggta gtaaggttgt aggaaatgca
     3901 gatagcgata gcaagttgag tatagatgca gaagcgagag cgaaagcacc gcaacctgtc
     3961 gaatttgata gctctattcg tcataatgga gatatagtgt tgacgctccc aaataatgcg
     4021 gatacagcaa ctatcaacta tccagtgagt gatacagagc tgaaaactgc tactgcaact
     4081 aaggggcaag acggtagctg gaggctttca cctgactcag gtcttgagct tgctagcaca
     4141 aatggagtca ctaagataac gctagcatat acaaaattga gtggcaatcg tacagtgact
     4201 gcaagtgcta aagcaggaag tggtgaggga gagagtaaag agcgtacctt tactaaaaca
     4261 gttccagcac acacaactcc gacaacccaa aacgttgtca tcgctgcaaa tgcgacacca
     4321 actgatgacc aattattgac aggtgtaata gctaacaata aacaatccgt cgcagctaaa
     4381 caaacacaaa ctgctattgc tgcaggaact acaaaagaaa ttccagcaac tttgacctac
     4441 acggatggtt cgactgaaga aatcactata acggttcagt ctaagccgac agcaccgacg
     4501 gtgaatggtt tagaaagtcg tgcaagggtt gccgtaccgg gtcttttatc aactgctcgc
     4561 acgattacag gtcaggctat gcagggtgca gaaaaagtta aattaacttt acaaaatggt
     4621 agtgagaaag aagtagatgt ggctgccgat ggtagttggt cttatacgct tgcagcagat
     4681 gaattcctga ctcaaacaat atcaaatagt aatgctaaat atagttctac gaaaatccgt
     4741 cttgtgcaag tgaaagatgg tctagaatct gaaccaaaag acattgacat tttgatgggg
     4801 cgcgccacta ttgatacgcc acttcgagct ggacgtgata tcacccttca cattccgcat
     4861 gacaccactt ctggctatat ccgcatagga ggttcggtag aacgaggtgg agtagatatc
     4921 ggactcaaga aggtgaacgg tgtctggact cttgatacag atgccaatag ggcaagtaag
     4981 ttagaacttg tgagtgagac agacccgacg aatccagctt tgaccaaggt tacgctcaaa
     5041 gttaaagcca cggataatgc ctcgtacagt ccaccgttta ccattggagg tgacagtgga
     5101 aacgtgaaat tcagagctca ctattacagt ggtagagata tcggtgcagt agttccgatg
     5161 gggagacaag gtcaatttga atggattttg tctggtcaac caaccaatac tagacctaca
     5221 gttggctggg aaactggtaa agaaatccaa gatggtcaaa agattccatc acctacagta
     5281 gatgagttga aagacttctt taaaggggag gatgctgaag atgatgcagg tctcactgta
     5341 ggatactcag cctctaatag aggtaaatta cgtgttcgtt tatatacaag tggaacgaac
     5401 caaactgtta gaacaaatgt ccgaggacga atcgatccag gtaattatag actgctcttg
     5461 tctactattg atgctgcagg ggtggagtct aatatccttg aaagaaatgt catcattcaa
     5521 agttatgcag attactatcg tgacgctgtt cagtatccaa ctactgctca aaaggtaacc
     5581 tacagtgata cagatatcac taatggtaac ttcacagcag cagccaaaac acgttttaag
     5641 gataaaatag agactatcaa tcgccaaaat acccaacttc caactagtgt gacttatagc
     5701 gtaggaacta cggatgataa ggcgaaagta gctgtgatta acttcccgga tggttctact
     5761 attgatattt ctcattcgca ggtagccaaa ccagatgtac cgacaatcac tccgacggat
     5821 acagaacaac aggggatgcc gaaggtaact gatgctgata gagaaatttc tggtactgcc
     5881 cttcaaaatg cgacaaaagt gacattgaag ttgcaaactg gtaaagggat agagattgta
     5941 aaagaaatta cagttaataa tacaaaagat ttcgctgcct tagtaccagg agaagggctc
     6001 ttaaaaaatg gagtatggaa gtataagctg gctgacgggc aatacttacg tcaaacagat
     6061 gcgacagcag aaccaggctc gtcctcactc ccattaaaag caacacaaac tgtatttgat
     6121 gcagttagtg atgatacgag tatctatgtt gccaataaac gtactgtaga aggtaaaaaa
     6181 attgagggag aagttggcag tcctgacttg aaaaaataca tagataaacc gcaagatgcc
     6241 gttgtctata aagaaaaagg gcaagaaaag ccattcccaa gtgactttat tgccaaatgg
     6301 gaaaatactc ctaactttga taccgttgga acctttacct ataaagtaaa attttatgaa
     6361 aaagacggca atacaccggc tgatcacatt agtgaaggtg ttgaagttac ctttgtagtc
     6421 aaatcaaaag caccagcaat cttagactac actaatagag ataacggtga aactcttgtg
     6481 aatattccgc aagatgctga tgaagttgtt ttcagtatcc ttagaagtga tacagctgta
     6541 gatactataa ctgtaaaaaa atctgaaggt tggacggcaa cagggattca aaaacgtgat
     6601 aacacttggg tatttccagc taactcagta catggcaacc gaacagtaac agcagttgcg
     6661 acagcaggga agggtgatac caagagtgta gagatgctga cattgattac aacacttgct
     6721 catgatgtta ctgttaatga aattactaaa cctactaaag gaaatccaac atcccaagat
     6781 ttgcttgatg cagtagaagt aactaataag aaatccgtag cactgaaaca aggagaaaat
     6841 tatccagaaa cttttggaac tcatcaaatc cacttgattg taacctatca agatgatagt
     6901 acagaagaga aggtagctaa ttatacagtt ccagatacaa gagatgcagc taaagatgac
     6961 attgatacga aagctacggc taagaaaaat gacatcgaag cagacgacaa gttgacgact
     7021 gaagagaagc aagcagctaa ggataaggta gatgcggcag ctcaaagtgc taaagatgcg
     7081 gttactgccg caactaccaa cgatggtgtg actcaagctc agaatgatgg aactactgct
     7141 gttaacaatg ttgatacaac gccaaaagcg aaaccatcag ccaaggcaga aattgatgcc
     7201 aaggttcaag atgccaacaa tgccattgac cagaatcaag atctgacgga cgatgagcgt
     7261 agagaggcta agaaagcagt gaaggacgca gctgatgaag ctaaggctaa tattgacaag
     7321 gcgacatctg caggagagct cacaactgct aagaacaacg gtcttgataa gttgaatgag
     7381 ccacaagacg caagcgttaa acaaaaagct aaagatgaca ttgatacgaa agctacggct
     7441 aagaaaaatg acatcgaagc agacgacaag ttgacgactg aagagaagca agcagctaag
     7501 gataaggtag atgcggcagc tcagtctgga acaaatcccg tgactccacc atctactaca
     7561 agaagtcgca gaactcctcc gcgccgctct cgtagaagtg taggatttgt agggaattcc
     7621 caaacaggta caactccatc ggctgtggac aagtcagagc ttcagtctct ggtagaggac
     7681 ctggagcgtc gcctacaaga tttagcagat ttatctcctg aagcacttga ggaagcacag
     7741 agtatcttga gagaggcgca agtggctctt gctaatgact ccttaacagc tcaagaatta
     7801 acggaactcc ttgccaaagt tagacaggct cttaattcta tacaagctgg cacatcagct
     7861 gataagagtc cgtctacgtc aaactcaaat aaggaagcag aaagcgccaa ggaacctaca
     7921 aatgcgacag atgtccctct atatggtgtc tttggagctg cagtactctc tcttctagga
     7981 gctctcctct ttgcagtggc tcgtaagaaa aactctcaac tagataagct ttcaagagaa
     8041 ttggatcaac ttctagttga gttagaggct agtgacaagg ataaaaaagg tttaggcaag
     8101 gctaaaaaat tagctaagaa agcaagaacc tttgtggata gccagcaaaa agatcctcaa
     8161 aaagaagcag aactaatttc tgaaatcaag actattctta gtcaactcag agaaggagtc
     8221 tagtcttgaa gaaaacttac tttcaaaaac gaagtgagta ggtttgtagc gatgatgagg
     8281 aaataaaaaa tctccagatt aggaaccatc agtgagttct ctagtctgga gatttttcgg
     8341 ctctttgtca actgtagtgg gttgaagtca gctaagctcg agaaaggaca aatttcgtcc
     8401 tttctttttt gatgttcaga gcgataaaaa tccgtttttt gaagttttca aagtttcgaa
     8461 aaccaaaggc attgcgcttg ataagtttga tgagattatt ggtcgcttcc agtttggcat
     8521 tagaatagtg tagttgaagg gcgttgacaa tcttttcttt atctttgagg aaggttttaa
     8581 agacagtctg aaaaata
//
LOCUS       DAVKDZ010000082         8230 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000082 DAVKDZ010000000
VERSION     DAVKDZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.083"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..755
                     /locus_tag="VWZ30_001806"
     CDS             <1..755
                     /locus_tag="VWZ30_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9335489.1"
                     /translation="FHTLLDKVLRTCKEFGVNAIVEYFGEEDTISNSIISSTGSLVDG
                     VIVFYESVDDIRIQYLKKNHMPFLVFGESQTSGVVYVSNNNFQATYDMMKAVTEEKFK
                     NMWLLMGGESHVNKDRERGVRSFLNDKNYFMDLKVIYGLSTIDSVYSYAMQHLTTQNY
                     PDIIFVSGDEKVQGLIRACYEKGILIPDDISIIGFDNIPISQYYTPALSTIAPNYVKL
                     AKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     misc_binding    967..1196
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1356..1436
                     /locus_tag="VWZ30_001807"
                     /pseudo
     CDS             1356..1436
                     /locus_tag="VWZ30_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            1425..1568
                     /locus_tag="VWZ30_001808"
     CDS             1425..1568
                     /locus_tag="VWZ30_001808"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335490.1"
                     /translation="MDRTDEVSSKHCFEVADRTDEVSNHTYDKATLTRFEEFFEEYKG
                     VPR"
     misc_binding    1637..1868
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1940..3301
                     /gene="trpE"
                     /locus_tag="VWZ30_001809"
     CDS             1940..3301
                     /gene="trpE"
                     /locus_tag="VWZ30_001809"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU9335491.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDLLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEVLEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVINPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLPELIAMDALKSTLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            3298..3864
                     /locus_tag="VWZ30_001810"
     CDS             3298..3864
                     /locus_tag="VWZ30_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU9335492.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEEVVK"
     gene            3875..4879
                     /gene="trpD"
                     /locus_tag="VWZ30_001811"
     CDS             3875..4879
                     /gene="trpD"
                     /locus_tag="VWZ30_001811"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU9335493.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            4876..5643
                     /gene="trpC"
                     /locus_tag="VWZ30_001812"
     CDS             4876..5643
                     /gene="trpC"
                     /locus_tag="VWZ30_001812"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU9335494.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALAEERLKELYDYATVLGLEVLV
                     ETHNLAELEVAHRLGAQIIGVNNRNLTTFEVDLQTSVDLAKHFKDDCLYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            5630..6229
                     /locus_tag="VWZ30_001813"
     CDS             5630..6229
                     /locus_tag="VWZ30_001813"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU9335495.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPSGVKKVGVFVSPSRVELLEVIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            6207..7430
                     /gene="trpB"
                     /locus_tag="VWZ30_001814"
     CDS             6207..7430
                     /gene="trpB"
                     /locus_tag="VWZ30_001814"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU9335496.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7423..8199
                     /locus_tag="VWZ30_001815"
     CDS             7423..8199
                     /locus_tag="VWZ30_001815"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU9335497.1"
                     /translation="MPKTLTEKLNAIKATGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVKPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
BASE COUNT         2407 a         1564 c         2000 g         2259 t
ORIGIN      
        1 ggtttcatac gctgttagat aaagtgttac ggacgtgtaa agaatttggt gttaatgcta
       61 tagttgagta ctttggagag gaggatacaa ttagtaattc gattatatca agcacaggta
      121 gcctagtaga tggtgttatt gttttttatg aaagtgtaga tgatatcaga attcagtatt
      181 taaagaagaa ccatatgcct tttcttgttt tcggtgaatc tcaaacgtct ggagtagtct
      241 atgtatctaa taataatttt caagctactt atgatatgat gaaagctgta actgaagaaa
      301 aatttaaaaa tatgtggttg cttatgggag gagaatccca tgttaataag gatcgtgaaa
      361 gaggtgtgcg ttcttttctg aatgataaaa attactttat ggatttaaaa gtcatttatg
      421 gcttatctac aattgattca gtttattctt atgcaatgca gcacttgact acccaaaatt
      481 atccggacat tatttttgtt tcaggagatg aaaaggttca gggcttgatt cgtgcgtgct
      541 atgagaaagg aattcttatt ccagatgata tttctattat tggatttgat aatattccta
      601 tttctcagta ttatactcca gctttgtcta caattgctcc caattatgtt aaattggcta
      661 aagaaatgat agaaggtgtt ttagcaatta ttaaagggga aagtgtcaca tctgttgaag
      721 tttctcctaa atttgttagg agacagagtt tctagtattg tttaattctt gaaaatccta
      781 gtggaaaaga tggagtgttc taaaataagt tctgtttagc ctccattcag tttctattga
      841 gttggatcca atttctaaca agtgggacat gttcatgttc gatctaagga agacatggtc
      901 acattcgaat gatattttca aaaaaatcgt aaaaaagtct tgacaaagaa aaaacaaagt
      961 attaaaataa gttcaacaaa tcagaaaagt aactattttt gatcttcagg gagcctgtgg
     1021 tggttgtgaa taggtggttg gaagtagtga aagtgggctg attttgaaaa tgaatttgaa
     1081 acaatgaaaa ttcggtgcgc acaccttaca gtgcagcttg ttgttaaaca aggcagagat
     1141 gtaaagggat agtcccttta taattgaggt ggcaccgcgt taccaacgcc ctcacacgga
     1201 agtatatttt gtgtgtgggc ttttttctat ccgtcgtttg gtttatcttt tattagggcc
     1261 ttaagtcgct ttgatgaact tgagttctat ctacagctcc ttgcctacta ctaaaagcaa
     1321 acaaaaagct ggactaatac gaaaaaagct agtaatactc aatgaaaatc aaagagtaaa
     1381 ctaggaaact agccgcaggt tgctcaaaac actgttttga ggttgtggat agaactgacg
     1441 aagtcagctc aaaacactgt tttgaggttg cagatagaac tgacgaagtc agtaaccata
     1501 cctacgataa ggcgacgttg actcggtttg aagagttttt cgaagagtat aaaggtgtac
     1561 cgagatgaat atctcttcat caatgtctct ggtcgtggag gcaaggacct agctgcgatt
     1621 gcagattact agaagctaaa aaataataga tggaaaaatt acagttcttt ctctcacaga
     1681 gagcttgtgg ttgctgaaaa caagcagaga aagctgtaaa gttgggttca tctgaaacga
     1741 gagaagaaaa catcattctc aagggagtgc cctttatcgc acagcctgtt acaggaggta
     1801 tctgagatag gaatgagaga taggagaaat cctataattg aggtggcacc gcgaatttcg
     1861 tcctcacgca agttattttg cgtggggatt tttcatacag tcgccacgaa ctagaattag
     1921 aactgaaagg gaaattacaa tggaacgaat cattcatgga gatgtcttat caccaatctt
     1981 ggcttatatg cgcttaaagg ggcaacataa ggttatttta gagagtattc cgagagacaa
     2041 ggaaacagct cgtttttcta tcctagccta taatccagtt tttgagatta agtttgaaaa
     2101 tggagttctc tatcaaaatg gtcaagtgat tgatcgggat cccttggatc tcctttatga
     2161 agtgattcat aagagtcagc accactcaga gctacctttt ggtggtggag ccattggttt
     2221 tgtgggttac gatatgattt cgctttatga agaaattggt caaatccctg aggatacaat
     2281 tgggacgcca gacatgcatt tctttgtcta tgagagctat atggtctttg accacaagaa
     2341 ggagaagata catgtcatag aggatgctct ctatagtgag cgcagtcaag aggtcttgga
     2401 aaaatccttg aaccaagtgc ttgaggaatt acgcattcct gctccaaatg aatttgaaga
     2461 cttggattta tcaccacttg acttcaaacc gcatatcgct ccacataagt ttgagggaat
     2521 ggtagaaaca gctcgtgact tgattcgtaa tggtgatatg ttccaatgtg tactcagcca
     2581 gcgtttctca gcagaagtta ctggaaatcc atttgacttc tacagaaatc tccgcgtgat
     2641 caatccatcc aattacctct atttctatga ttttggggat tatcaaatca tcggagccag
     2701 tccagaaagt ttggtttctg tcaaaaatgg catcgtgaca accaatccga ttgcagggac
     2761 gcgaccaaga ggggctacgg atgaagaaga caaggccttg gcgactgacc tcctctctga
     2821 tgagaaggaa acagcagagc atcgaatgtt agtagacctg ggtcgtaacg atattggccg
     2881 catctctgaa acggccagtg tccaagtcac taagtatatg gaagtggagc tcttccgtta
     2941 tgttatgcat ctgaccagcg tggtcaaggg gcgtttgctt ccagaactca ttgctatgga
     3001 tgccttgaaa tcaacgcttc cagctggaac cgtttctgga gcaccaaaaa ttcgagctat
     3061 gagacgcatc tatgaactgg aaacggaaaa acggggcgta tatgcaggag caatcggcta
     3121 cttgtctgcg acgggtgata tggatttggc cattgccatt cgaaccatga ttctcaaaaa
     3181 tcaaagagcc tatgtgcagg ctggggcagg gattgtctac gactctatcg cccaaaacga
     3241 ataccaagaa accattaaca aagcaaaatc tatgactaga attggagaac taagaccatg
     3301 attttattga ttgacaacta tgattctttt acctataact tggcccaata catcgggaat
     3361 tttgcagaag ttcaggtttt gagaaatgat gattccaagc tgtatgaaga agctgaaaaa
     3421 gcagatggtc tggtcttttc tcctggtcct ggttggccag ttgatgctgg aaagatggaa
     3481 gacatgattc gtgattttgc aggcaagaag ccgattctag ggatttgttt gggccaccaa
     3541 gccattgcag aagtctttgg tggtaagcta ggtttggctc caaaagtcat gcatgggaaa
     3601 cagagcaata tcaactttga agcgccatct gttttgtatc aaggtattga ggatggacgt
     3661 gcggtcatgc gttatcacag tattttgatt gaggaaatgc cagaagactt tgaagtgaca
     3721 gctcgttcga ctgatgacca agccatcatg gggattcaac ataaaaacct accgatttat
     3781 ggcttccagt accatccaga gagcattgga acgccagacg gcttgtcttc tattcggaat
     3841 tttatcgagg aggttgtaaa gtgaggaaac taggatgaaa gagattattg aaaaactagc
     3901 aaaatttgaa aatttatcag gtgtggaaat gacggatgtc attgagcgta tcgtaactgg
     3961 gcgtgtaact gaagcgcaga ttgcttctct cctcttagct cttaagatga agggggaaac
     4021 acctgaagaa cgcacagcca ttgcccaagt catgagagga catgcccagc atattccaac
     4081 tgagattcat gatgccatgg acaactgtgg tacaggtggg gacaagtctt tcagttttaa
     4141 tatttccaca actgcagcct ttgtcttggc tggtggcggt attcacatgg ccaagcatgg
     4201 taaccgctcg atttcttcta aatctggttc cgcagatgtc ctcgaagcct tgggaatcaa
     4261 tcttgacctc aaaccagctg aactaggtaa ggtctttgat aaaactggaa tcgtctttct
     4321 cttcgctaaa aatatgcacc cagctatgaa atacatcatg ccagctcgtt tggaattggg
     4381 aattccaacg attatgaact tgactggtcc actgattcat ccaatggctt tagaaacaca
     4441 gcttcttgga attagtcgtc cagagctcct agaaagtaca gctcaggttt tgaaaaatat
     4501 gggtcgcaaa cgtgccatcg tggttgctgg accagaaggg ttggatgaag ctggcttgaa
     4561 cggaacaacc aagattgcac ttcttgaaaa tggcgaaatc agcttgtcaa gctttactcc
     4621 agaggatttg ggaatggaag gctatgctat ggaagatatt cgtggtggga atgctcagga
     4681 aaatgcagaa attttgctta gcgttctgaa aaacgaagca agtccattct tggaaacgac
     4741 agtcttgaat gctggtcttg gtttctatgc taatggtaag atcgatagca tcaaggaagg
     4801 agttgccttg gcccgtcaag tgattgctag aggcaaggcc cttgaaaaac tcagactgtt
     4861 acaggagtac caaaaatgag tcaggaattt ttagcacgaa tcttagagca gaaggcgcgt
     4921 gaggtggagc agatgaagct ggagcaaatc cagcctctgc gccagaccta tcgcttggca
     4981 gaatttttga agaatcatca ggaccgcttg caggtaatcg ctgaggtcaa gaaagctagc
     5041 cctagtttgg gagatatcaa tctcgatgtg gatattgtgc aacaggccca gacttatgaa
     5101 gaaaacggag cagtgatgat ttcggtgttg acagatgagg ttttctttaa agggcatttg
     5161 gattatctac gggaaatttc cagtcaggta gagattccga cgctcaacaa agactttatc
     5221 atagatgaaa agcaaatcat ccgcgctcgc aatgcaggtg cgacagtcat cttgctcatt
     5281 gtggcagcct tggcagaaga acgtcttaag gaactttacg actacgcgac agtgcttggt
     5341 ctggaagtct tggtggaaac tcacaatcta gctgaactag aagtggctca cagacttggt
     5401 gcccagatta tcggggtcaa taaccgcaac ttgaccacct ttgaggtcga cttgcagacc
     5461 agtgtagact tggctaaaca tttcaaagat gattgcttgt acatttctga atctgctatt
     5521 ttcacagggc aggatgcgga acgactagcc ccatacttta acggaatttt ggtagggaca
     5581 gctcttatgc aggcagaaaa tgtggcccag agaatcaagg agttgcagat tgacaaaggt
     5641 taagatttgt ggactatcga ccaaagaagc ggtggaaaca gccgtttcag caggagccga
     5701 ctatatcggt tttgtctttg cacctagtaa aagacaggtg actttagaag aggcagctga
     5761 gctggcaaag cttattcctt caggtgtgaa aaaggttgga gtatttgttt caccaagtcg
     5821 ggtagaactg ctagaagtca ttgacaaagt tggcttggac ttggttcaag ttcacggtca
     5881 ggtggcagat gatttatttg agaatttgcc ttgtgccagt attcaggctg tgcaggtaga
     5941 tggaaatggg catgtcccca attctcaggc agattatcta ctctttgatg cccctgtggc
     6001 aggaagtggt cagtcctttg attggggtca actggatacg actggactag cacagccctt
     6061 ctttatcgca ggtggcctta atgaagataa tgtagtaaaa gcaattcaac attttactcc
     6121 ctatgcagta gatgtatcga gcggagtgga gacagatgga caaaaagatc atgaaaagat
     6181 tagaagattt atagagaggg taaagcatgg catatcagga accaaataaa gatggatttt
     6241 acggaaaatt cggcggacgt tttgtcccag aaacattgat gacagcagtt ttggagttgg
     6301 agaaggccta ccgtgaaagt caggcagacc caagtttcca agaggaatta aaccaacttt
     6361 tgcgccagta tgtgggacgt gaaactcccc tttactacgc aaaaaacttg acccagcata
     6421 tcggcggagc caagatttat ctcaaacggg aagaccttaa ccatacagga gcccacaaga
     6481 ttaacaatgc cttaggacaa gtttggcttg ccaaacgcat gggtaaaaag aaaattatcg
     6541 cagaaactgg tgctggtcag cacggtgtgg caactgcaac tgctgcggcc ctctttaaca
     6601 tggaatgtac catctacatg ggtgaggaag atgtcaaacg ccaagccctc aatgtcttcc
     6661 gtatggagct tttgggagct aaggtcgagg ccgtgacaga tggttcgcgc gtgctcaagg
     6721 atgcggtcaa tgcagctctt cgttcatggg tggctaatat agatgatacc cactatatcc
     6781 ttggttctgc cttggggcct catccatttc cagaaattgt tcgtgacttc caaagtgtca
     6841 ttggtcgaga ggctaaacaa cagtaccgtg acttgacagg acaaaatctg ccagatgccc
     6901 tagtagcctg tgttggtggt gggtcgaatg ccatcggtct cttccatccc tttgtagaag
     6961 atgagtcagt agccatgtat ggagctgaag cggctggact tggtgtggat acggagcacc
     7021 acgcagctac cttgaccaag ggtcgtccag gtgtccttca cggttccctc atggatgtgc
     7081 tccaagatgc ccatggtcaa attcttgaag ccttctctat ctcagcaggt ttggactatc
     7141 ctggtatcgg tccagagcat tctcactacc acgatattaa acgtgccagc tatgtccctg
     7201 tgacagacga agaagccttg gaaggattcc aactcttgtc tcgtgtggaa gggattatcc
     7261 cagccttgga atctagccat gctatcgcct ttgcggtgaa attggccaaa gaacttggac
     7321 cagaaaagtc tatgattgtc tgcctatcag gtcgtgggga caaggatgtg gttcaagtca
     7381 aagaccgctt ggaagcagat gcagcaaaga agggagaagc tcatgcctaa gacactaaca
     7441 gaaaaattga atgctataaa agcaactgga aaaggaattt ttgttcccta tatcatggct
     7501 ggtgaccacg agaaaggttt ggacggtctt gctgaaacaa ttcacttttt agaagatttg
     7561 ggtgtctctg ctattgaagt gggtattccc ttttcagacc ctgttgcaga tggacctgtt
     7621 atcgaagaag ctggcttgcg cagtttagcc cacgggacct ctacccaggc tttagttgaa
     7681 accttgaaaa ccattgaaac agagattcca ctggtcatca tgacctactt caaccccctc
     7741 tttcagtacg gtgtggagaa ctttgtcaaa gatctggctg atacagctgt gaagggcttg
     7801 attatcccag acttgcctca tgaacatgcc aactttgtaa aaccattttt ggcagataca
     7861 gatatcgcct tgattccttt agtaagcttg accacaggaa ttgagcgcca gaaagagttg
     7921 attgaaggcg cggagggatt cgtctatgcc gttgccatca atggggtgac agggaaatca
     7981 ggcaattacc gtgcagattt ggacaagcac ttggcgcaac ttcatcaagt ggccgacatc
     8041 ccagtcttga caggttttgg tgtatctagt caagccgatc tggaacgctt caatgcggtg
     8101 tcagatggtg ttatcgtcgg ttcgaaaata gtaaaagctc tccaccaagg agagccgatt
     8161 caggatttta tcagacaagc agtagcttac caaaaataat caaacaagta gcaagtaaga
     8221 ggaaaggcac
//
LOCUS       DAVKDZ010000083         8201 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000083 DAVKDZ010000000
VERSION     DAVKDZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8201
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.084"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(235..594)
                     /locus_tag="VWZ30_001816"
     CDS             complement(235..594)
                     /locus_tag="VWZ30_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEU9335498.1"
                     /translation="MDMILMKLASILLLILTLVVCIIITKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSVLAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLVMYIEMIVELMAQK"
     gene            686..1615
                     /locus_tag="VWZ30_001817"
                     /pseudo
     CDS             686..1615
                     /locus_tag="VWZ30_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003023668.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            1618..2175
                     /locus_tag="VWZ30_001818"
     CDS             1618..2175
                     /locus_tag="VWZ30_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9335499.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVAKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDIERDAVLDFVSQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            2168..2422
                     /locus_tag="VWZ30_001819"
     CDS             2168..2422
                     /locus_tag="VWZ30_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335500.1"
                     /translation="MDKQYLREKLDAMRQNFVESTHHERAVGVLDQVHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFEFCCKKD"
     gene            2434..4488
                     /locus_tag="VWZ30_001820"
     CDS             2434..4488
                     /locus_tag="VWZ30_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU9335501.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVKAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLTLIALTFVLFAIRFVMIYGYF
                     ACRTRRLKKKLNKYMKDMLLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLSSRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKNKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDIVELVR
                     RN"
     gene            4655..5515
                     /locus_tag="VWZ30_001821"
     CDS             4655..5515
                     /locus_tag="VWZ30_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU9335502.1"
                     /translation="MKKWWKELTDKPLVKSFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFVKDILPESLYEGVEKISREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMVSLLVSFGLQLLFAFALFLSLFGQMIL
                     DLLAHYWTKDGIIYQSLQGLAGPLIYVLLFAILVMLYYFLPNLSNRKLSYTLPGSAFV
                     LLVILGLLTLFSSYLNYYIHRLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            5726..6436
                     /gene="ccdA2"
                     /locus_tag="VWZ30_001822"
     CDS             5726..6436
                     /gene="ccdA2"
                     /locus_tag="VWZ30_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9335503.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            6456..7022
                     /locus_tag="VWZ30_001823"
     CDS             6456..7022
                     /locus_tag="VWZ30_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU9335504.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSMTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKDAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTQPGFMEKDAILQTLKELA"
     gene            7033..8145
                     /gene="msrB"
                     /locus_tag="VWZ30_001824"
     CDS             7033..8145
                     /gene="msrB"
                     /locus_tag="VWZ30_001824"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9335505.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVN"
BASE COUNT         2333 a         1532 c         1823 g         2513 t
ORIGIN      
        1 ataatactct tcgaaaatct cttcaaacca cgtcagcttc gccttgccgt atatatgtga
       61 ctgacttcgt cagtcttatc tacaacctca aaacagtgtt ttgagtagcc tgcggctagc
      121 ttcctagttt gctctttgat tttcattgag tataagaatt attgaaaaag atataaagaa
      181 aaacacttca atcactgtat ttcagtttga ttgaggtgtt ttagatttag ttttttattt
      241 ctgggccatc aattcaacaa tcatctcgat atacatgacc agggttaata agaatcctgt
      301 acgccagaag aatttgatga atttaggata gtaaaagcta cgttttttca aaaggaagaa
      361 aaaaacgaga ataatggcta gaactgagag ggctagcccc agtctaggga ggaaattatg
      421 ggtaaaggtt ttagctgtga tcaggtagta ctcaaatacc aagactggaa aggccaaatc
      481 cgcaaagttt cgtcctagtt tttttaatct aaaaagtttg gttatgataa tgcagactac
      541 tagagtcagt atcaataata aaatagatgc taatttcatt aaaatcatat ccatattgta
      601 tcataaaaaa aggctaatgg aaacgagaaa cagaggaatt tataggaaag tcaggctttt
      661 gagattgtgg gtgatttttg ttataatgag agttatgaaa tcttataata ccttgaatga
      721 ttattatcga aaactctttg gagaaaagac ctttaaagtc cctattgatg cgggatttga
      781 ctgtcccaat cgtgatgaaa ctgtggctca tggaggctgt actttttgta cggtttccgg
      841 ttctggagat gccattgtgg caccagatgc gcctatccgt gagcaatttt ataaggaaat
      901 tgactttatg catcgtaagt ggccaggtgt tcagaagtat ctggtttatt ttcaaaattt
      961 tacaaacacc catgaaaagg tggaagttat tcgagagcgt tatgaacagg ctatcaatga
     1021 cccaggtgtg gtaggaatca atattggaac gcgcccagac tgtttaccag acgaaaccat
     1081 cgaatatttg gctgagttgt cggagcgcat gcatgtgagg gttgaattag gcttgcagac
     1141 tacttatgaa gcaacctctg acctgattaa ccgcgcgcat tcttatgaat tgtaggtgga
     1201 aacggtcaag cgtttgagaa agtatccaaa aattgagatt gtttcccatt tgatcaatgg
     1261 tttacctggt gaaactcatg agatgatggt tgaaaatgtc cgccgttgtg tcacggataa
     1321 cgatattcaa gggattaaac tgcatttgct tcacctgatg accaatacac gtatgcaacg
     1381 agattatcac gaaggacgtt tgcagttgat gagccaggac aaatatgtta aggtcatctg
     1441 tgaccaactg gaaatcattc ccaaacatat tgtcatccat cgaattacag gagatgcacc
     1501 gagagatatg ctgattggtt ccatgtggag cctaaataaa tgggaagtgc taaatgctat
     1561 tgaaactgaa atgcgacgtc gtggaagtgt tcaaggatgt aaaacaggag tttgaaaatg
     1621 aaaagaccac ttgagatggc acatgatttt ttggctgagg tcgtgacaaa agaggatgtc
     1681 gtagtggatg cgactatggg aaatggtcat gacacgcttt ttttagccaa gctagccaag
     1741 caagtctatg cctttgatat tcagaagcaa gccttggaaa agacccaaga gcgtttgcat
     1801 caggctggct tgacaaatgc ccagttaatc ttgcaaggcc atgagacact ggaccagttt
     1861 gtggcaaaag ctaaggcagg gatttttaat ctgggctatt tgccgtcagc tgataagtct
     1921 gtcatcaccc gaccgcagac aacgattgag gcattagaaa agctatgtgg cttacttgtc
     1981 aaaggtggac gaattgctat catgatctac tatggtcatg aaggaggcga catcgagagg
     2041 gatgctgtct tggattttgt tagccagttg aaccaacaag agtacacagc tgccatttac
     2101 cgaactttaa accaagtcaa caacccacca tttttagtaa tgattgaaaa attagagaga
     2161 tacagacatg gataaacaat acctacgtga aaagctggat gccatgcgcc agaattttgt
     2221 tgaatcaacc caccacgaac gagcggtggg cgtgctagac caagtgcata tgagcaaaaa
     2281 aatgcttaaa atcaagaaaa aattagttgc tcttgaaatg gaacggtgcc agagaaaaat
     2341 tgagcacaaa gattgttcca agattgacca aaaaatcaaa gagcagaagg agatatttga
     2401 attctgttgt aaaaaagatt aaggaggacg tgcgtggaat tactgattta cctcatccta
     2461 tttttactgg tcttgattgt ctcaagtaca accaataagc tcctaccctt tttgcctctc
     2521 cctttggtgc agattctttt gggaattgta attggtctct ttttacccaa tactgacttt
     2581 caccttaata cggagttgtt tttggcactg gttatcggac ccttgctttt ccgagaggct
     2641 gaagaagcag atgttaaggc tattttaaaa cactggcgaa tcattgttta tctcatattt
     2701 ccagtgattt ttatctcgac cctgagtttg ggtggcttgg cccatcttct ttggttcagc
     2761 cttcccttgg cagcttgctt ggctgttggg gcagcccttg gtcctacgga cttggtggcc
     2821 tttgcctctc tttcggagcg ttttagcttt cctaagcgcg tgtccaatat tcttaagggc
     2881 gaaggactct tgaatgatgc ttctggtttg gtggcttttc aggtagcttt gacagcttgg
     2941 acaactggag ctttttctct ggggcaagct agcagttcgc tcatcttttc aatcctaggc
     3001 ggttttttaa ttggattttt aacagccatg accaaccgct tcctccatac cttcttgcta
     3061 agtgtgcgcg caacggatat tgccagtgaa cttttactag aattgagttt gcctctagtg
     3121 accttctttc tggcagaaga agtccacgtt tcagggatta ttgccgtcgt agttgctggg
     3181 attttaaagg caagtcgctt caagaaaatc acgctcctcg aagcccaagt ggacacggtg
     3241 accgagacgg tctggcatac agtgaccttt atgctcaacg gttctgtctt tgtgatttta
     3301 gggatggagc tggaaatgat agcagagcct atcttaacca atccaatcta taatccctta
     3361 cttttattgc taactcttat agcccttacc tttgtcctct ttgctattcg ttttgtcatg
     3421 atttatggct attttgcctg taggacccga cgcctcaaga aaaagctaaa taagtatatg
     3481 aaggacatgc ttctcttgac cttttcaggt gttaagggaa cggtgtcgat tgctacgatt
     3541 ctcttgatac caagtaatct agaacaggag tatcctctct tgcttttcct tgttgcaggt
     3601 gtgacgcttg tcagcttttt aacaggtctc ttggtcttgc ctcatctttc tgatgaagag
     3661 gaagaaagca aggattatct catgcatatc gccattttga atgaagtaac gctagagttg
     3721 gaaaaagagt tggaagacac cagaaataaa cttcccctct atgcggctat tgacaattat
     3781 catggacgta ttgaaaatct cattttaagc caagaaaacc aggatgatca agaagactgg
     3841 gctgctttga aactcttgat tcttagtatt gaaagtgatg gtttggaaca ggcctatgaa
     3901 gaggggaaca ttagcaatcg tgcttaccga gtttaccaac gttatctgaa aaatatagaa
     3961 caaggaatca atcgtaaact ttcctcaaga ctaacctatt atttccttgt ttctttgcgg
     4021 attttacgtt ttctccttca tgaagttttt actctcggaa agaccttccg tagctggaag
     4081 aacaaggagc aaagccgtct ccgtgctctt gattatgacc aaattgcaga gctctatctt
     4141 gccaatacag agatgattat tgaaagtttg gaaaacctga agggagtcta cagacgctct
     4201 ttgattagtt ttatgcagga gtctcgtctt cgagaaacag ctattatcag cagtggtgcc
     4261 tttgtcgaac gggttatcaa tcgtgtcaaa cccaacaata tcgatgaaat gctgagaggc
     4321 tattatctgg agcgcaagtt gattttcgaa tacgaagaaa aacgattgat tacgactaag
     4381 tatgccaaga aattacgaca aaatgtaaat aatttagaga actattcctt gaaggaagct
     4441 gccaataccc tgccgtatga tatagtggaa ttggtaagaa gaaattagtt aatactcttc
     4501 gaaaatctct tcaaaccacg tcagcgtcgc cttaccgtat gtatgagagt cttatctaca
     4561 acctcaaaac agtgttttga gcagcctgcg gctagcttcc gagtttgctc tttgattttc
     4621 attgaggata agattgtaag tgaaggagtg tgacatgaag aagtggtgga aagagctgac
     4681 agacaagcct ttagtaaaat cctttttgca ttattatcaa gcatcagata gtgagttgac
     4741 cagtgtcgca gtagcctact attggttgat ttcgattttt cccctgcttt tggtggtggt
     4801 caatatcctc ccctattttc agattcctgt gggagaattt ctgggatttg tgaaagacat
     4861 cctacccgaa agtctctatg aaggtgtaga aaaaatatcc agagaagtct tgacgcaacc
     4921 ttcaacaggt ttattgagct tttctgtctt atcggcgtta tggagctttt caaaatctat
     4981 gaatttcctg caaaaagctt ttaacaaggc ttatggcgtc gaaaagagtc gcggacttat
     5041 ttcccatcag atggtgagtc tcttagtgag ttttggtttg caactactat ttgcttttgc
     5101 cttgttttta agcttatttg ggcagatgat tttggattta cttgctcatt attggacaaa
     5161 agatggcatt atctatcagt ctttacaagg gttggcaggc cctctgattt acgtcctact
     5221 ctttgctatc ttggtcatgc tttattattt tcttcccaat ctatctaaca ggaaactgag
     5281 ctatacccta ccaggcagtg cctttgttct cttagtgatt ttaggtttac taactttgtt
     5341 ttctagttat ctcaattact acatccatcg tttagtggac gtccgaattt taggttccgt
     5401 tcttcttgtt gttatgatgt tttggtttat cttgattgct aaaattgtta tcctaggcgc
     5461 ggttatcaat gccagtatgc agagtttgaa agatccggtc tttaaaaaag actaactatt
     5521 tatccattac aaaagcgcat agtatcaaga ctttatagaa ccttgatact atgcgctttt
     5581 gtgctttgac agttttttgt atagtttttg tccaagattt ctaaatcatc tgattaaatt
     5641 cctattttcc cctatcattg tccctgtttt ttcgaaaatg gaaggagtat aataaatcta
     5701 tcaataaaat gataggagga actttatggg tcatattttc ttttttctaa gtgtcttttt
     5761 ggcagggatt ctatccttct tttctccttg tatcttacct ttgttaccgg tctatacagg
     5821 ggtgttacta gatgataagg atggtgctca ggcttctagc ggcaaatttt caatctcagt
     5881 tactagttta ttacgaactc tggcctttat agcaggaatt tcctttatat ttattttgtt
     5941 gggctatgga gctggttttt taggcgattt gctttatgct tcttggttcc aatatcttac
     6001 tggggcaatt attatccttc ttggtttgca ccaaatggag attctacact ttaaggggct
     6061 ttataaggaa aagaggctac aactgcaagg acaggggcaa aatggtaagg gctatagtca
     6121 ggcattttta ttgggcttga cctttagttt tgcttggacg ccttgcgtgg ggccggttct
     6181 ggggtctgtt ttggccttgg cggcttcagg tggttcagga gcttggcagg gagctggtct
     6241 catgttggtg tatacgctgg gcttggcgct accattcttg cttctagctc tgacctctag
     6301 ttatgttttg aaacatttcc gaaaacttca tccctatctc ggaatcctca aaaaagtggg
     6361 tggttttctc attattgtga tgggactctt ggttctgttt ggaaatgctt caattttaag
     6421 tcaattattt gaataaaatg gaaaggaata tcaatatgaa aaaatggcaa acatgtgttc
     6481 ttggagcagg ttcgctcctt tgtttgacgg cttgttcagg caagtccatg actagtgaac
     6541 accaaacgaa agatgaaatg aagacggagc agacagctag taaaacaagc gcagctaaag
     6601 ggaaagaggt ggctgatttt gaattgatgg gagtagatgg caagacctac cgtttatctg
     6661 attacaaggg caagaaagtc tatctcaaat tctgggcttc ttggtgttcc atctgtctgg
     6721 ctagtcttcc agatacggat gagattgcta aggatgctgg tgatgactat gtggtcttga
     6781 cagtagtgtc accaggacat aagggagagc aatctgaagc ggactttaag aattggtata
     6841 agggattgga ttataaaaat ctcccagtcc tagttgaccc atcaggcaaa cttttggaaa
     6901 cttatggtgt ccgttcttac ccaacccaag cctttataga caaagaaggc aagctggtca
     6961 aaacacaacc aggattcatg gaaaaagatg caattttgca aactttgaag gaattagcct
     7021 aggaggtgtc ctatgaatga taagttaaaa atcttcttgt tgctaggagt attttttcta
     7081 gccataaccg gtttctatgt tctattgata cgaaatgcag ggcagacaga tgcctcgcaa
     7141 attgaaaagg cggcagttag ccaaggagga aaagcagtga aaaaaacaga aattagtaaa
     7201 gacgcagact tgcacgaaat ttatctagct ggaggttgtt tctggggagt ggaggaatat
     7261 ttctcacggg ttcccggggt gacggatgcc gtttcaggct atgcaaatgg tagaggagaa
     7321 acaaccaagt acgaattgat taaccaaaca ggtcatgcag aaaccgtcca tgtcacctat
     7381 gatgccaagc aaatttctct caaggaaatc ctgcttcact atttccgcat tatcaatcca
     7441 accagcaaaa ataaacaagg aaatgatgtg ggaacccagt accgtactgg tgtttattac
     7501 acagatgaca aggatttgga ggtgattaac caagtctttg atgaggtggc taagaaatac
     7561 gatcaacctc tagcagttga aaaggaaaac ttgaagaatt ttgtggtggc tgaggattac
     7621 caccaagact atctcaagaa aaatccaaat ggctactgcc atatcaatgt taatcaggcg
     7681 gcctatcctg tcattgatgc cagcaaatat ccaaaaccaa gtgatgagga attgaaaaag
     7741 accctgtcac ctgaggagta tgcagttacc caggaaaatc aaacagaacg agctttctca
     7801 aaccgttact gggataaatt tgaatccggt atctatgtgg atatagcaac tggggaacct
     7861 ctcttttcat caaaggacaa gtttgagtct ggttgtggct ggcctagttt tacccaaccc
     7921 atcagtccag atgttgtcac ctacaaggaa gataagtcct acaatatgac gcgtatggaa
     7981 gtgcggagcc gggtaggaga ttctcacctt ggccatgtct ttacggatgg tccacaggac
     8041 aagggcggct tacgttactg tatcaatagc ctctctatcc gctttattcc caaagaccaa
     8101 atggaagaaa aaggctacgc ttatttacta gattatgtta attaatactc ttcgaaaatc
     8161 tcttcaaacc acgtcagcgt cgtcttaccg tatgtagggt t
//
LOCUS       DAVKDZ010000084         7981 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000084 DAVKDZ010000000
VERSION     DAVKDZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7981
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.085"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(116..349)
                     /locus_tag="VWZ30_001825"
     CDS             complement(116..349)
                     /locus_tag="VWZ30_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9335506.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQSVGDVVKIIQGK"
     gene            complement(355..1347)
                     /gene="plsX"
                     /locus_tag="VWZ30_001826"
     CDS             complement(355..1347)
                     /gene="plsX"
                     /locus_tag="VWZ30_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU9335507.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIVGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(1344..2114)
                     /gene="recO"
                     /locus_tag="VWZ30_001827"
     CDS             complement(1344..2114)
                     /gene="recO"
                     /locus_tag="VWZ30_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU9335508.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(2111..3280)
                     /locus_tag="VWZ30_001828"
     CDS             complement(2111..3280)
                     /locus_tag="VWZ30_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9335509.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGHYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            3429..4439
                     /locus_tag="VWZ30_001829"
     CDS             3429..4439
                     /locus_tag="VWZ30_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEU9335510.1"
                     /translation="MSFLSKNGAGIFVCLLISIVSWYLGGFFPVIGAPVFAIFMGMLL
                     HPFLSSYKQLDVGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLATLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNDGFALFAGTAVNDTSSVTATASAWDSLYQTNTLESATIVKLT
                     RTLAIIPITLFLSYWQSREQKNKQGLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIIMAMSAIGLKTNLVAMVKSSGKSIVLGAVCWIAIILTSLGMQTLIG
                     IF"
     gene            complement(4552..4632)
                     /locus_tag="VWZ30_001830"
                     /pseudo
     CDS             complement(4552..4632)
                     /locus_tag="VWZ30_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4667..5104)
                     /locus_tag="VWZ30_001831"
     CDS             complement(4667..5104)
                     /locus_tag="VWZ30_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU9335511.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(5189..7822)
                     /gene="polA"
                     /locus_tag="VWZ30_001832"
     CDS             complement(5189..7822)
                     /gene="polA"
                     /locus_tag="VWZ30_001832"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU9335512.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDY
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYGGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
BASE COUNT         2218 a         2052 c         1456 g         2255 t
ORIGIN      
        1 gaggatatat cttcatgagc ccttgataac cactgtcagc caagatttta ccagcttgtc
       61 cgatatttct catttctaaa aaccatctac ttccgttgac ttggaactcc gattgctatt
      121 ttccttgaat gattttaacc acatctccta cactttggag ttggtcaatt tcctcatcgc
      181 tgatttcgat actaaattca tcttccagag tcaagataaa ctccatcaag tcaacagaat
      241 ccgcatccaa atcgtctttc agactcaagg attctgtcac gacaaagtcc tctccctgtc
      301 gctcttggat aatggtcaca atacggtcaa aaatttcttt ttctgtcatc tcttttattc
      361 tcctgaaaat tcacgcgcag tctgggcaac cacgtctgtt tctagcatgg tacgaatctg
      421 acgaatcgta ctataaacag ccttggcatc gcttgagcca tgagtcttga caacaggtgc
      481 cttaacacca aacaataccg ctccaccaac atctgaataa ttgagctgtt ttttcaaacc
      541 actgaggctg tccttgagaa ggagggcacc tagtttcgct cgaagaccac cacctgtaat
      601 agctgtcttg agcaagccca cgattcccat agctgtccct tcgatggatt tgagcacagc
      661 gtttcccgtg aaaccatctg ccacaacaac atctgcaacg ccattcatca aatcacgcgc
      721 ttccacgttt ccgataaagt tcaaactttc atcagccgcc agtaattcat aagtttcctt
      781 acgaagcggg tcgcccttgc tactctctgt tccgttgttg agcaaaccaa cgcgtggttg
      841 cgcaatgcca cggacatttt tagcatagaa ggaacctaga accgcatatt gatggaggtg
      901 ctgggctgta ttttctgcat tggcaccaag gtctagcatg tcaaaacctt ttccatcaac
      961 ggtaggcaag gtagacatga gtccaggacg gtcgatattc ttgatacgac ccacgatgaa
     1021 gaatcctgct gccaacaagg cacctgtatt cccagccgaa aggacagcgt ctgcttcacc
     1081 atctttgaca gccttggctg ccaataccat actggcattt ttcttattcc taatagctct
     1141 cgtaggttca tcatccgaat caatcttctc atccgtatgg ataatgctga cgcgctctgt
     1201 cgctgtcaga tattgcttga ttttagcttc atctccgtaa agttgaacct cgatatctga
     1261 aaagtcagat agggcttgat tgacaccctc aacaatggcc tgaggtgcgt aatcgccccc
     1321 catggcatct actgcgattt ttttcatttc ttttcctctt ttagtaattg tccccagtct
     1381 gctagggaat caataaattt ctttgatttt aggtgaatcc caacgtactc ttcatataat
     1441 tgatccataa attggcgtag ctcttgcttg attccaggct tgagcgaaat ggtctccaaa
     1501 gtctcaaaat caatagcttg aaattgattg agcagatagg ggatattggg attgagatga
     1561 caacgtctct tatcctcatg ataatgctct ggacagaggc aggctccata tttgaaagaa
     1621 aagtcaaaag cctgaccaac ccgatggcag aagacacact cattaaaatt gaggctgatt
     1681 ccaaatcgag tcaaaatttg aatttcaaaa atattggtca aaacctgata atccaagcct
     1741 gcttccatca actccaaagt cttttgcaaa aaagcaaaca agggagcatc ctgctgattg
     1801 tcctgcaaac tagcatctgc aagagctgcc acataggtcg cataggccat gacaaagagg
     1861 tcactattaa tcttgggaaa agtcatgacc tcatgatagt cttcgatgta actgagtccg
     1921 tcatcattga ttcgcaagag aaatcgtgcc agcaccaagg gctgaataac aggcgccagc
     1981 ttagactgac cagcgtgttt gacaaaaaac atgcgtttgc caacctgctc tgtaaaaatt
     2041 ttgacgagct tgtcatcctc acgaaaattg cgattgtaaa gcaccaagcc ttgactcgtg
     2101 atagactgaa tcatgcttct ctcatgtact cctcaagtcg tttcatggct tctttgatag
     2161 tctccatgct ggctgcataa gataggcgga cgtagccttc cccgtaatgt ccaaaggctg
     2221 caccagggat aaaggcaacg gccttcttct gagcaaaatc cttcagaaaa gcaaaggagt
     2281 cttgattgta gcccgctgga attttagcaa aaatatagaa ggcaccgtct ggtttgataa
     2341 tctcaaaacc aagagcagtc attttttcga tgatatagtc ccgacgctgg atatattcct
     2401 tcttcatggg ctccgcatca tttttaccag ccgtcaaggc ttctaccgca gcatgttgcg
     2461 ccatggtatt tgcggcagtg accaagtatt ggtgactctt gattaactgg gctgtgaagg
     2521 ccgcaggagc gaaaatcagc cccaaacgcc aacctgtcat ggcatgcgat ttagacaaac
     2581 cattgataat aatagcctgg tctctcaaca tcgttcctag agacacatgg gcttcgcctg
     2641 tgtaggtcaa ttctgagtaa acctcatcac agacaacaaa aatttcgtac ttgcgtaaaa
     2701 cagctgccaa ggcctctaac tgctctcgac tgtaggtaat tcctgtcgga ttggctggat
     2761 agttgagaat aaccgccttg agcttatcac cctgctccaa aatggccttc tccaacatct
     2821 caggagtcaa gacaaaacca ttttcagtcg tatcaatctc aacaatttct gccccaacta
     2881 agttaacaat cggttcatag cctggataag caggagctgg caaaagtacc ttgtctccct
     2941 cttccaaaat agccgtcaaa gtcgcagata aagcctctgt cgccccaatt gtaaccaaga
     3001 tttcattttc aggagcatag tccagttggt acttttcctt aacaaagtca ctggctgcct
     3061 gacgtagagt cagcagacca ctcatccctg tatagtagga ttggttctga tcaatcgctc
     3121 gcttggccgc ctccttgaca tggtctggcg ttgtaaaatc aggttccccc aaggtcaaac
     3181 gcaagacccc aggaatctcc gaaataacct ggtcaaactg acgaatcaac gaaacttgaa
     3241 ttttatctaa ctgtttatta aagcgcttag ttaagtccat agatacctcc tactttcaaa
     3301 acgtatctct attatactac aaaagccccc cgaccgcaaa aatacattag agaatagctt
     3361 tccactttct attttacttt tcctgtttac aggtctataa tggtaacaga ttctatttgg
     3421 aggtttttat gtcatttcta tcaaaaaatg gagcaggcat ctttgtctgc cttctcattt
     3481 ctatcgtatc ttggtactta ggaggattct tccctgttat tggcgcaccc gtttttgcga
     3541 tttttatggg catgctccta catccctttc tctcgtctta taaacaactg gatgttggat
     3601 tgacctttag ttccaagaaa ttgctccagt atgccgttat cttacttggt tttggtctca
     3661 atatctctca agtcttcgca gttgggcaat cttcactccc tgtcatcctt tccaccattt
     3721 caatagcctt gattgttgcc tacctcttcc agcgcttctt tgccctggat acaaaactgg
     3781 ctaccttgat tggagtaggt tcttctatct gtgggggttc tgccattgca gcgacagcgc
     3841 ctgttatcca tgctaaggaa aaggaagtag cccaagccat ctccgttatc tttttcttca
     3901 atgtcttggc tgcgctcatc tttccaaccc tcggcacctg gcttcatcta tccaatgacg
     3961 gcttcgccct ctttgcagga actgcggtca atgatacttc ctctgtaacc gccacagcta
     4021 gcgcttggga cagtctctac cagaccaata ccctcgaatc cgcaaccatt gtcaaactca
     4081 cgcgcaccct agctatcatc cccatcactc tctttctctc ctactggcaa agtcgcgagc
     4141 aaaaaaataa gcaaggctta caactgaaaa aagtcttccc actttttatc ctttacttca
     4201 tccttgcatc gctcctaacc acccttctga cctctctcgg tgtgtctagt agtttcttta
     4261 cccctctcaa acaactctct aaattcctca ttatcatggc catgagtgcc atcggtctca
     4321 aaactaatct ggtcgctatg gtcaaatcca gtggaaaatc cattgttctc ggagccgtct
     4381 gctggattgc catcatcctc accagccttg gtatgcagac ccttatcggc attttctaat
     4441 actcttcgaa aatctcttca aaccacgtca acgtcgcctt gccgtatata tgttactgac
     4501 ttcgtcagtt ctatctgcaa cctcaaaacg gtgttttgag ctgacttcgt cagtcgtatc
     4561 tacaacctca aaacagtgtt ttgagcagcc cgcggctagt ttcctagttt gctctttgat
     4621 tttcattgag tataacacaa aaggtagccc atcagctacc tttttcttat gcttcctcaa
     4681 tcaagcgagt atgttctctc ttgatacagc gattcatcac gatatcatca catccaccat
     4741 cacgcaaaat ctctttcgct tctaaacttt caagtcctag ctgtgcccaa aaaatcttgg
     4801 catcagcttt gagaaaatca cgcgccacat cgggcagaaa ttcactgcga cgataaacat
     4861 tgacaatatc tacaggaaaa ggaatttcag cgaggctagc ataagccttt tcacccaaga
     4921 tttcgccacc tgccgccttg ggattgactg ggatgatttt atagccccga gcctgcattt
     4981 cctttgtcac tcgattgctg gttgtttctt cacggtcaga caaacccacc acagcaaggg
     5041 ttttactcgt tgcgagatac tgacgtatca cgccatcact tggattgata aattcttgac
     5101 tcatagaaat cctccttttt catcagtata gcacattttg aaaaggtttg cagaattcta
     5161 ctacaaaaaa ggaggactat cccccttttt atttagcctc gtaccaggtt gctccttcat
     5221 tctcatctgc gataagagga acactgagtt gaatggcttc ttccatggtt tgtttcacca
     5281 attttttcat ctctaccaat tcagatttag gcacttcaag gacgatttca tcgtgcactt
     5341 gtaacagcat cttagtctga taaccacctg caaccaaggc tttatccagc tgaatcatgg
     5401 caatcttgag aatatctgct gccgaaccct ggataggtga gttgatagca gttcgctccg
     5461 caaaaccacg aatattgaag ttgcgcgaat tgatatctgg caactcacgg cgacgcttaa
     5521 agagggtctc tacatagccc ttatcacgcg cctcccgcac cacttcatcc atgtagtttt
     5581 taatacctgg aaaacgttca aagtaggtat caatgtaggc tttggcttcc ttacgactaa
     5641 ttcccaaatt attagacaag ccaaagtctg aaatcccata aaccactcca aagttaactg
     5701 ccttggcatt gcgacggtca tttgcagtca cattatcagg acgctcaatg ccaaagaccc
     5761 gcatggctgt cgaagtatgg atatctgccc cctcttggaa ggccttaatc aagtgctcat
     5821 ccttagaaat atgcgccaaa acgcgcaatt caatctgtga atagtcagag ctgagtagca
     5881 cactatcctc ccactctggc acaaaagcct tccgaatcaa gcgcccttgt tccaatcggg
     5941 caggaatatt ttgcaagttt ggatccacac tagacaaacg cccggtctgg gtcaaatcct
     6001 gcatatagcg agtatgaatc tttccatcag ccaaaatcca gtcctgcaag ccaattacat
     6061 aagtagattg aatcttagca atttgacggt aatccaggat tttcttaaca atcggagcaa
     6121 taggagcgag acgctccaac acatccactg ctgtcgaata acctgtcttg gttttcttag
     6181 tgtattctag aggaagtccc aatttctcaa agagaagcac gcccaactgc ttaggcgagt
     6241 tgacattaaa ctcctcacca gccagctcgt aaatctcttg agtcagtttt tcaatgacaa
     6301 gctcattttc agcctgcatc tcaagcaagg tctctttctt gaccacaatc ccagcaattt
     6361 ccatcttggc aagaacaaaa gccagaggtt gctccatatc ataaagaagc tctaattgcc
     6421 cattttcgct gagtttttca agtaaaatag gctctgtttc taccaaaaca gcaagtttac
     6481 aagctaagtg ttccaaaaat ttctcacgtt caggaatggc ctttttaaca cccttaccgt
     6541 agaaagtttc atcatcaacc aagtaagtct gaccataaag actagcgatg gtcgcaattt
     6601 cattgtcctc cacagtcgaa aggaggtatt tagccaaacg gatgtcaaaa gcaggcgcct
     6661 gcaaatccac accaaaacgt tgcaaaagaa ctttaacctt cttaaagtca taaactctca
     6721 gagatgtttt ttctaagaaa tccttgaaaa tcgggtcttg caacagctca agcttgtctg
     6781 tggcatagag cttatcccca caagaccagg caaatccaac caaattatcc gtatggtaat
     6841 tctcaccaaa aagctcaaag tggaagatag actcttcact cagcatatct tgactgattt
     6901 ggtcaacaat agtaaaatcc aaaccctcag ccacatcagc tgacgacaca tttaaagcct
     6961 gcttgagctg tttgaatccc atctcatcgt aaaatttccc aagattttca acatctggac
     7021 caccatagac caagtcctct aaaccaatcg caatcggtgc cttggtatca atggtcgcta
     7081 gtgttttaga caaaaaggcc tgttccttgt cattgatgag attttccttc atcttagaag
     7141 tcttcattcc atcaatattt tcataaatcc cctcaagcga accatgctcc agcaagagct
     7201 taatacccgt cttttcaccg actttggtca ccccagggat attatccgac ttatcaccca
     7261 tgagcgcctt gagatcgata aactgagctg gtgtgaggcc catttcttcc atgaggtaat
     7321 ctggcgtaaa ggcctcaaac tcagccacac ctttcttgga aatttcaacc accgtatgct
     7381 catccgtcag ctgaatcaaa tccttgtccc cactgacaat ggtaatatca aaaccatcct
     7441 gctctgctag cttatccagc gtcccaatga tgtcatccgc ctcatactga gccagctcat
     7501 agtgacgaat ccccatataa tccagcaact cacgaatgaa aggaaattgc tcacgaaact
     7561 catcaggagt cttggcccga ccacccttat agtccgcata catctctgtc cggaaggtcg
     7621 tctttcccgc atcaaaagcc accaaaatat gactcggctc aacccgctcc aacaaatgac
     7681 tcaacatcaa ctgaaaacca taaatcgcat tggtatgcaa accagccgca ttcttaaaac
     7741 ggtccaactg ctgatacagc gcaaaaaacg cccgaaaagc tacagaagac ccatcaatca
     7801 ataataattt tttcttatcc atacacccat tataaaggaa agaatcaaaa aataccattg
     7861 ggaagagcta gagcaagtat ttttcaaact ttttccgaat aaatagatag agccagagaa
     7921 tttagtaaac ctagatttaa aaatgtgcta taatatagta tattgaatct ataatagtac
     7981 a
//
LOCUS       DAVKDZ010000085         7981 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000085 DAVKDZ010000000
VERSION     DAVKDZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7981
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.086"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            37..1797
                     /gene="dnaG"
                     /locus_tag="VWZ30_001833"
     CDS             37..1797
                     /gene="dnaG"
                     /locus_tag="VWZ30_001833"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU9335513.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFMEAVQILGQRVGIEVEKPLYSEQ
                     KSASPHQALYDMHEDAAKFYYAILMTTTMGEEARNYLYQRGLTDEVLKHFRIGLAPPE
                     RNYLYQRLSGQYREEDFLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDIPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VREKSITAQNSYIHILADSLASFDYAQIEQIVNESRQAQRQNRMEGVSRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERSWYQVLAQDLPAEMSPRELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            1800..2909
                     /gene="rpoD"
                     /locus_tag="VWZ30_001834"
     CDS             1800..2909
                     /gene="rpoD"
                     /locus_tag="VWZ30_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU9335514.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVVPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            2923..3252
                     /locus_tag="VWZ30_001835"
     CDS             2923..3252
                     /locus_tag="VWZ30_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9335515.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            3367..4410
                     /gene="cpoA"
                     /locus_tag="VWZ30_001836"
     CDS             3367..4410
                     /gene="cpoA"
                     /locus_tag="VWZ30_001836"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU9335516.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGISREKVSYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPVALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            4426..5751
                     /locus_tag="VWZ30_001837"
     CDS             4426..5751
                     /locus_tag="VWZ30_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9335517.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            5780..5956
                     /locus_tag="VWZ30_001838"
     CDS             5780..5956
                     /locus_tag="VWZ30_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9335518.1"
                     /translation="MRSGRDKKIAGVCAGVAHYFDMDPTIVRVIWGVLAFGYGAGVIA
                     YIILWIIAPVVTDY"
     gene            6015..6149
                     /locus_tag="VWZ30_001839"
     CDS             6015..6149
                     /locus_tag="VWZ30_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU9335519.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            6213..7523
                     /gene="obgE"
                     /locus_tag="VWZ30_001840"
     CDS             6213..7523
                     /gene="obgE"
                     /locus_tag="VWZ30_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU9335520.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEDFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGGDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            7533..7691
                     /locus_tag="VWZ30_001841"
     CDS             7533..7691
                     /locus_tag="VWZ30_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335521.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            complement(7742..>7981)
                     /locus_tag="VWZ30_001842"
     CDS             complement(7742..>7981)
                     /locus_tag="VWZ30_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9335522.1"
                     /translation="KQVHPIFQTVFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLI
                     KRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT         2394 a         1410 c         1882 g         2295 t
ORIGIN      
        1 tataaagtaa actatgataa catggaggta ttgtgtatgg ttgacaaaca agtcattgaa
       61 gaaatcaaaa acaatgccaa cattgtggaa gtcataggag atgtgatttc tttacaaaag
      121 gcaggacgga actatctagg gctctgtcct tttcatggtg aaaaaacacc ttctttcaac
      181 gttgtagagg acaagcagtt ttaccactgt tttggttgtg gtcgctctgg tgatgtcttt
      241 aagttcatcg aggagtacca aggggttccc tttatggaag ccgtccaaat cttaggtcag
      301 cgtgttggaa ttgaggttga aaaaccgctt tatagtgaac agaagtcagc ttcgcctcac
      361 caagctcttt atgatatgca cgaagatgca gccaaatttt attacgctat tctcatgaca
      421 acgactatgg gagaagaggc gagaaattac ctttatcagc gtggtttgac agatgaagtg
      481 ctgaaacatt ttcggattgg tttggcacca ccagaacgaa actatctcta tcaacgtttg
      541 tctggtcagt accgtgaaga agatttcttg gattcaggcc tgttttacct ctcggatgcc
      601 aatcaatttg tagacacctt tcataatcgc attatgtttc ccctgacaaa tgatcaggga
      661 aaggtcattg ccttctcagg tcgtatctgg cagaaaacgg attcacaaac ttctaagtat
      721 aaaaatagcc gatcgactgc aatttttaac aaaagttacg aattatatca tatggatagg
      781 gcaaaaaaat cttctggtaa aaccagtgag atttacctca tggaaggatt catggatgtc
      841 attgcagcct atcgggctgg aatcgaaaat gctgtggcat ctatgggaac ggccttgagt
      901 cgtgagcatg ttgagcatct gaaaaggtta accaagaagt tggttcttgt ttacgatggc
      961 gataaggctg ggcaagccgc gactttgaaa gcgctggacg aaattggtga tatacctgtg
     1021 caaattgtca gcatgcctga taacttggat ccagatgagt atctacaaaa aaatggacca
     1081 gaagacttgg cctatctatt aacgaaaact cgtattagtc cgattgagtt ttacattcac
     1141 cagtacaaac ctgaaaatag tgaaaatctg caggcccaga ttgagtttat tgaaaaaata
     1201 gctcccttga ttgttcgaga aaaatctatt actgctcaaa acagctatat tcatatttta
     1261 gctgacagtc tggcgtcctt tgattatgct cagattgagc agattgttaa tgagagtcgt
     1321 caggcgcaaa ggcagaatcg catggaaggg gtttccagac cgacgccaat cactatgcct
     1381 gtcaccaagc agttatcggc tattatgagg gcagaagctc atctacttta tcgaatgatg
     1441 gaatctcctc ttgttttgaa cgattaccgt ttgcgggaag actttgcatt tgccacacct
     1501 gaatttcagg tcttatatga cttgcttggt cagtatggca atcttcctcc agaagttcta
     1561 gcagaacaga cagaggaagt tgaaagatct tggtaccaag ttttagctca ggatttacct
     1621 gctgagatgt caccgcggga actaagtgaa gtagaaatga ctcgaaacaa ggctctcttg
     1681 aatcaggaca atatgagaat caaaaagaag gtgcaggaag ctagccatgt aggagatacc
     1741 gacacagccc tagaagaatt ggaacgttta atttcccaaa agagaagaat ggagtaataa
     1801 tggcaacaaa acaaaaagaa gtaacaacat ttgacgtaca agtagcagaa tttatccgta
     1861 atcataagca aaaagggaca gcaacagatg atgaaatcaa tgctagtctt gtcgttcctt
     1921 ttaccttgga cgctgatggg attgaagatc tcttgcaacg gattcaggat gcgggaattt
     1981 ctatcacaga taacgaagga aatccaagtg cgcgtgttct tagcaatgaa gaagaaccag
     2041 aactcagcga tgaggacttg attgggtcaa cttctgctaa ggtcaatgac cctgtccgta
     2101 tgtacttgaa agaaataggg gtcgttcctc tcttgaccaa tgaagaggag aaagagttgg
     2161 cactggctgt tgaagctggt gatatcgaag ccaaacaacg tcttgcggaa gccaatcttc
     2221 gtttggttgt ttccattgcc aaacgctatg tcggtcgtgg catgcagttc cttgacttga
     2281 ttcaagaagg aaatatgggc ttgatgaagg cggttgacaa gtttgactat tctaaagggt
     2341 tcaagttttc aacttatgca acttggtgga ttcgtcaggc tatcactcgt gctattgcgg
     2401 atcaagctcg taccatccgt atcccagttc acatggttga aactatcaat aaattggttc
     2461 gtgaacagcg gaatctcctt caagaattgg ggcaagatcc gacaccagaa cagattgctg
     2521 aacgaatgga tatgacacct gataaggttc gtgaaatctt gaagattgcc caagaaccag
     2581 tatctcttga aactcctatc ggtgaagagg acgatagcca ccttggagac tttatcgaag
     2641 atgaagtgat tgaaaatcca gtggattata cgactcgtat cgtcttgcgt gagcaattgg
     2701 atgaaatctt agatactctt acagaccgtg aagaaaatgt tctgcgtcta cgttttggac
     2761 tagatgatgg aaaaatgcgc acacttgaag atgtggggaa agtctttaac gtaactcgtg
     2821 agcgtatccg tcagattgaa gcaaaggctt tgagaaaact acgccaacca agtcgcagca
     2881 aaccgcttcg tgattttatt gaagactaag agtgaggaaa atatggctta tacagaagag
     2941 caaattgaaa acatcaaaac acggatttta acagccttgg aagaagtcat cgaccctgag
     3001 ttgggaatcg atattgtcaa tcttggtttg atctatgaga ttcgttttga cggtgacaca
     3061 gggcaaacag agattgacat gactttgaca actatgggtt gtcccttagc agaccttttg
     3121 acagaccaga tttatgatgc tatgatagag gttccagaag taacggatac tgaggttaaa
     3181 ttagtctggt atccagcctg gacagttgaa aaaatgagtc gctatgcccg cattgcccta
     3241 ggcattaagt aaacatataa aagcatgtga gagactgttg gaaaagcgag gaaatttccc
     3301 ctcttttcct ctagtctctc ctttcttttg ctgattttat tcaaagaaaa tgatataata
     3361 gtagttatgg agaaaaagaa attacgcatc aatatgttga gttcaagtga gaaagtagca
     3421 ggacaaggag tttcaggtgc ttaccgtgaa ttagttcgtc ttcttcaccg tgctgccaag
     3481 gaccaattga ttgttacaga aaatcttcca atcgaggcag atgtgactca ctttcatacc
     3541 attgattttc cctattattt atcaaccttc caaaagaaac gctcagggag aaagattggc
     3601 tatgtgcatt tcttgccagc tacacttgag ggaagtttga aaattccatt tttcttaaag
     3661 ggaattgtga aacgctatgt attttctttt tacaaccgga tggagcactt ggttgtggtc
     3721 aatcctatgt ttattgagga tttggtagca gctggtattt cacgtgaaaa agtgagctat
     3781 attcctaact ttgtcaacaa ggaaaaatgg catcctctac cacaagaaga ggtagtcaga
     3841 ctgcgcacag atcttggtct tagtgacaat cagtttatcg tagtaggtgc tgggcaagtt
     3901 cagaaacgta aagggattga tgactttatc cgtctggctg aggaattgcc tcagattacc
     3961 tttatctggg ctggtggctt ctcttttggt ggtatgacag atggttatga acactataag
     4021 aaaattatgg aaaatccccc taaaaatttg atttttccag gcattgtatc gccagagcgg
     4081 atgcgcgaat tgtatgctct agcggatctt ttcttgttgc ctagttacaa tgagctcttt
     4141 cctatgacta ttttagaagc tgcgagttgt gaggctccta ttatgttgcg tgatttagat
     4201 ctctataagg tgattttgga gggaaattat cgggcgacag cgggtagaga agagatgaaa
     4261 gaggctattt tggaatatca agcaaatcct gttgccttaa aagatctcaa agaaaaggct
     4321 aagaatattt ccagagagta ttctgaagag catctgttac aaatctggtt ggacttttat
     4381 gagaaacaag ccgctttagg gagaaagtaa aaagtgaggt aatctatgcg aattggttta
     4441 tttacagata cctattttcc tcaggtttct ggtgttgcga ccagtattcg aaccttgaaa
     4501 acagaacttg aaaagcaggg acatgctgtt tttatcttta cgacgacaga taaggatgtc
     4561 aatcgctacg aagattggca aattatccgc attccaagtg ttcctttctt tgcttttaag
     4621 gatcgtcgct ttgcctaccg aggttttagc aaggcacttg aaattgctaa acagtatcag
     4681 ctagatatta tccatactca gacagaattt tctcttggcc tgttggggat ttggattgcg
     4741 cgtgaattga aaattccagt catccatacc tatcacaccc agtatgaaga ctatgtccat
     4801 tatattgcta aggggatgtt gatccggccg agtatggtca agtatctggt tagaggtttc
     4861 ctgcatgatg tggatggggt tatttgccct agtgagattg tccgtgactt gctatctgat
     4921 tataaggtca aggttgaaaa acgggtcatt cctactggga ttgaattagc caagtttgag
     4981 cgtccggaaa tcaagcagga aaatttgaaa gaactgcgta gtaaactagg gattcaagat
     5041 ggtgaaaaga cgttgcttag tctttcgaga atctcctatg aaaaaaatat tcaagcagtt
     5101 ttagcagcct ttgctgatgt tctgaaagag gaagacaagg ttaaactggt agtagctggg
     5161 gatggccctt atctgaatga cctcaaagag caagcccaga acctagagat tcaagactca
     5221 gtcatcttta cagggatgat tgctcctagt gagacggctc tttactataa agcggcggat
     5281 ttcttcattt cggcatcgac aagcgaaacg caaggtttga cctacttgga aagcttagcc
     5341 agtggaacac ctgtcattgc tcacggaaat ccttatttga acaacctcat cagtgataaa
     5401 atgtttggaa ccttgtacta tggagaacat gatttggctg gtgctatttt ggaagccctg
     5461 attgcaacac cagacatgaa cgagcatacc ttatcagaga aattgtatga gatttcagct
     5521 gagaactttg ggaaacgagt gcatgagttt tatctggatg ctattatttc aaataacttc
     5581 cagaaagatt tggctaaaga tgatacggtc agtcagcgta tctttaagac agttttgtat
     5641 cttccgcaac aggtggttgc tgtacctgta aaaggttcta gacgcatgtt gaaggcttca
     5701 aaaacacagt tgatcagtat gagagattat tggaaagacc atgaagaata gaaagaggaa
     5761 cagtatgaaa aaacaattaa tgagaagcgg tcgtgataaa aagattgctg gtgtttgtgc
     5821 tggggtagcc cattattttg atatggatcc tactattgtt cgcgtaatat ggggtgttct
     5881 tgcttttggt tatggagcag gagttatagc ttatatcatt ttatggatta tcgcacctgt
     5941 agtaactgac tattgaaaac tagctaaatt catgctaaga taatagaaaa tagaatgcaa
     6001 cgaaggagag aaaaatggca tttggagata atggaaatcg taaaaaaact atgtttgaga
     6061 aaataacctt gtttatcgtg attatcatgc tagtagcaag tttattggga atttttgcaa
     6121 ctgcaattgg tgccctcagt aatctataaa atagattcaa gaaaatttag tgactgggat
     6181 ttcccagccc ttttttaaag tgagaagaaa aaatgagtat gtttttagat acagctaaga
     6241 tcaaggtcaa ggctggtaat ggtggtgatg gcatggttgc ctttcgccgt gaaaaatatg
     6301 tccctaatgg tggtccttgg ggtggtgatg gtggtcgtgg aggcaatgtg gtcttcgttg
     6361 tagacgaagg actacgtacc ttgatggatt tccgctacaa tcgtcatttc aaggctgatt
     6421 ctggtgaaaa agggatgacc aaagggatgc atggtcgtgg tgctgaggac cttagagttc
     6481 gagtaccaca aggtacgact gttcgtgatg cggagactgg caaggtttta acagatttga
     6541 ttgaacatgg gcaagaattt atcgttgccc acggtggtcg tggtggacgt ggaaatattc
     6601 gtttcgcgac accaaaaaat cctgcaccgg aaatctctga aaatggagaa ccaggtcagg
     6661 aacgtgagtt acaattggaa ctaaaaatct tggcagatgt cggtttagta ggtttcccat
     6721 ctgtagggaa atcaacactt ttaagtgtta ttacctcagc taaacctaaa attggtgcct
     6781 accactttac cactattgta ccaaatttag gtatggttcg cacccaatca ggtgaatcct
     6841 ttgcagtagc cgacttgcca ggtttgattg aaggggctag tcaaggtgtt ggtttgggaa
     6901 ctcagttcct ccgtcatatc gagcgtacac gtgttatcct tcacatcatt gatatgtcag
     6961 ctagcgaagg ccgtgatcca tatgaggatt acctagctat caataaagag ctggagtctt
     7021 acaatcttcg cctcatggag cgtccacaga ttattgtagc taataagatg gacatgcctg
     7081 agagtcagga aaatcttgaa gactttaaga aaaaattggc tgaaaattat gatgaatttg
     7141 aagagttacc agctatcttc ccaatttctg gattgaccaa gcaaggtctg gcaacacttt
     7201 tagatgctac agctgaattg ttagacaaga caccagaatt tttgctctac gacgagtccg
     7261 atatggaaga agaagcttac tatggatttg acgaagaaga aaaagccttt gaaattagtc
     7321 gtgatgacga tgcgacatgg gtactttctg gtgaaaaact catgaaactc tttaatatga
     7381 ccaactttga tcgtgatgaa tctgtcatga aatttgcccg tcagcttcgt ggtatggggg
     7441 gtgatgaagc ccttcgtgcg cgtggagcta aagatgggga tttggtccgc attggtaaat
     7501 ttgagtttga atttgtagac taggagactg gtatgggaga taaaccgata tctttccgag
     7561 atgcggatgg taattttgtt tccgccgcag acgtttggaa tgaaaagaaa ttggaagaac
     7621 tatttaatcg tctcaatcca aatcgtgcct tgagattggc acgaactaaa aaggaaaatc
     7681 catctcagta aagaagctaa aaaatcccgg ctctttgtca actgtagtgg gttgaagtca
     7741 gctaagctcg agaaaggaca aatttcgtcc tttctttttt gatgttcaaa gcgataaaaa
     7801 tccgtttttt gaagttttca aagtttcgaa aaccaaaggc attgcgcttg ataagtttga
     7861 tgagattatt ggtcgcttcc agtttggcat tagaatagtg tagttgaagg gcgttgacaa
     7921 tcttttcttt atctttgagg aaggttttaa agacagtctg aaaaatagga tgaacctgct
     7981 t
//
LOCUS       DAVKDZ010000086         7866 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000086 DAVKDZ010000000
VERSION     DAVKDZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.087"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            132..827
                     /locus_tag="VWZ30_001843"
     CDS             132..827
                     /locus_tag="VWZ30_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEU9335523.1"
                     /translation="MSETNHEIDSNFAGRLNILRAGVLGANDGIISIAGVVIGVASAT
                     SNIWIIFLSGFAAILAGAFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDMELAKKSL
                     YAAYIQNGECETSAQLLTNKAFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFL
                     GSLPPMLSITIFPSEYRIPATVLIVGVALLLTGYTSARLGKDPTKTAMIRNLAIGLLT
                     MGVTFLLGQLFSI"
     gene            complement(1158..3146)
                     /locus_tag="VWZ30_001844"
     CDS             complement(1158..3146)
                     /locus_tag="VWZ30_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9335524.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVTDPLTALTTFFLAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNEGVKEQKALTLNDHQFSVKEEFTKDFIVNHVPNQF
                     NILTSDYNYLVVPNLQAFLDQFPDSAIYNQFYGGMNVSISEEEQLKVAEEYEKYLDQF
                     NAQLNTEGSYVYGSTLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQILTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     IDTTMMLIVTLSICAIFLIVYVLIFMITSRSYRKIVQM"
     gene            complement(3148..3906)
                     /locus_tag="VWZ30_001845"
     CDS             complement(3148..3906)
                     /locus_tag="VWZ30_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9335525.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPTRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            4334..4525
                     /locus_tag="VWZ30_001846"
     CDS             4334..4525
                     /locus_tag="VWZ30_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335526.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            complement(4879..5151)
                     /locus_tag="VWZ30_001847"
     CDS             complement(4879..5151)
                     /locus_tag="VWZ30_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9335527.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(5210..5659)
                     /locus_tag="VWZ30_001848"
     CDS             complement(5210..5659)
                     /locus_tag="VWZ30_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEU9335528.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTKRYRCGLCRGKLLLVNQPED"
     gene            complement(5646..7775)
                     /locus_tag="VWZ30_001849"
     CDS             complement(5646..7775)
                     /locus_tag="VWZ30_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEU9335529.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEVVLAKIQEQGKLTKELEEAILEAEKLADVEELY
                     LPYKEKRRTKANIAREAGLFPLARLILQNVADLEQEAEKFVCEGFATGKEALSGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQVKDESLDEKQVFQIYYDFSETVGNMQGYRT
                     LALNRGEKLGVLKVDFEHATDRILAFFAARFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASSESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
BASE COUNT         2312 a         1660 c         1512 g         2382 t
ORIGIN      
        1 ttcggtgttt gatctatcag aaaagatgtc gacattttta tatcattaaa atcgtgcaac
       61 ttttttcaga tacttttaaa agattgattt ccctatattt ttcctttata ctggataaaa
      121 aggagattag tatgtcagaa acaaatcacg aaatcgattc aaattttgca ggtcgtttaa
      181 atatcctgcg tgcgggtgtt cttggtgcta atgatgggat tatttccatt gctggtgtgg
      241 ttattggggt tgccagtgct acaagcaata tctggattat ctttttgtca ggttttgcgg
      301 ctatcttagc tggtgccttt tcaatggcag gtggagaata tgtatccgtt tcaactccaa
      361 aagataccga ggaagctgcc gtttcgcgag aaaaactctt gctagaccaa gatatggaac
      421 tagccaaaaa atctctctat gcagcctata tccaaaatgg agaatgcgaa acctctgccc
      481 aactcttgac caacaaggcc tttcttaaaa atccactcaa ggctctggta gaagaaaaat
      541 atgggattga gtatgaagaa tttactaatc cttggcacgc tgctatttct agcttcgttg
      601 cctttttcct tggtagctta ccaccaatgc tttcaattac cattttccca agtgaatacc
      661 gcatccctgc tactgtcctt atcgtcggtg tggcccttct tctcactggt tacactagtg
      721 ccagacttgg aaaggatcca accaaaacag ctatgattcg gaacctagct attggtctct
      781 tgaccatggg agttaccttt ttgctaggac aacttttcag catttaatac tcttcgaaaa
      841 tcaaattcaa accacgtcag cttcgccttg ccgtatatat ggttactgac ttcgtcagtt
      901 ttatctgcaa cctcaaaaca gtgttttgag ctgacttcgt cagttttatc tgcaacctca
      961 aaacagtgtt ttgagctgac ttcgtcagtt ctatctgcaa cctcaaagca gtgctttgag
     1021 ctgacttcgt cagttctatc tgcaacctca aagcagtgct ttgagcaacc tgcggctagc
     1081 ttcctagttt actttttgat tttcattgag tataaaatac aagaaatacc tcgattttga
     1141 agtcgaggta tcttttttta catttgtaca atcttgcgat agcttcttga agtaatcatg
     1201 aaaatcagca catagacgat gaggaagata gcgcagatag acaaggtcac aatcaacatc
     1261 atagtcgtat ctattacacc aatcactttt aaaatcagac taagcatatg gtaggcaaag
     1321 gctaaatgta tgaaggcaaa gagcaaagga aggaagaaaa ctgttaaaat ctgtttgttg
     1381 atggtttgct tgatttgctt ttgatctaaa ccaactttct gcaagataat aaagcgctca
     1441 cggtcttcat agccttcaga aatttgtttg tagtaaatga ccagaacggt tccgaccata
     1501 aagataatgg ataggaaaat accgataaag aagacaccac caaagagggc actcatctga
     1561 gcactagcat ctgctagagt gctaccataa acatagctac cttccgtgtt taattgagca
     1621 ttaaattgat cgaggtattt ttcatattct tcagcgactt tgagttgctc ttcttcactg
     1681 atacttacat tcataccgcc gtaaaactga ttatagatag ccgaatctgg gaattggtcc
     1741 aaaaaggctt gtaaattagg tacaacaagg taattgtaat cagaagtcaa aatattaaac
     1801 tgatttggaa catggtttac aatgaaatct ttagtaaatt cttcttttac agaaaattga
     1861 tgatcattta gagttagagc tttctgttct ttaactccct cattctttgc aaagagtccg
     1921 acctcatttc ctgatagaga cagtttttga ccagtcatat tttcataatc tttttggtca
     1981 aataccatga aaactgtttt gggttggaca cggttttgtc ctttttcaaa aatagttaac
     2041 ttggttcctt cttgatttgc aataccaaag ttactgtaac gaagtacttc tttctctttg
     2101 acactataac ctttgtcact tgcaaactgg ctcaagagtt tgtccaaatc ttctttttca
     2161 acattttgcc ctgaaacccc aaaatcatga ggatttagaa cttttttaaa gctttctgcg
     2221 gaattgaaaa tgcttgtcgc tgctgacatg gttaccaaaa ccattgttga caaaatagcg
     2281 atggttgcta gtccaaccgc atttttcttc atacggaaaa tcaagttgga aacagatatg
     2341 aggttattag gttggtaata gtatttcttg tttttcttta agatttgtag gaagactgta
     2401 atccctgcat taaacaatag ataagtacca aagataacca gcaaaacagc taggaagaaa
     2461 gttgttaggg ctgtaagagg atcggttacc gtaagggcaa gataatagcc aatccctaaa
     2521 cttatggaac caagaatcgt ttggagagga aggaagcgac cttttttctc tccgctagct
     2581 ttctcacgtg agagctggag ggcattcata cgggcgattc gaagggcatt caggaacatg
     2641 aggcctagga aaatcaaacc gaagacaaga agtactgtaa tgacaacttt catctggaag
     2701 gtagcgacca gttccacctt caatttcatc agtttgagta ggaaagcgaa aatcaacttg
     2761 tcaaacaagg ctccaatacc gattcctgct ccaacagtta gaatcccaaa tagcactaac
     2821 tccttaaagg tcatactgat tagatggcgc ttctccaagc ctaacatgcc atatataccc
     2881 agttccttgg aacggttttt catgacaaaa ctattggcat agaggacgat aatggctgac
     2941 gcaagggtaa cgacaaacat accaaatcca agtgttgctt gaatggtggt tcctccacgg
     3001 atttccgcaa tctttggatt gaaggttagg gagtaaaaga gataggtgat ggtgactgcc
     3061 aagagaacag ccagtgcaaa gggatagtag agtttgcggt ttttaatcaa gttcgatacc
     3121 gctaacttat tggttaatcg aaacatacta attcacctcg cttgccatga cagtcaaggt
     3181 atcagagatt tcttggaaca tctgacgctc tgtcttctct ccacggtaga tttggttgta
     3241 aagaatgccg tccttgataa agagaacacg cttggccctg ctagcagctg ctgttgagtg
     3301 ggttaccatg aggatggttt gcccacgctc attgatttca ttaaagacat caagtaaggc
     3361 tgcagatgac ttggaatcaa gggctcctgt tggctcgtcc gcaaggagaa tttcaggttc
     3421 tgtgatgatg gcgcgggcta ctgctacacg ctgtttctga ccaccagaaa tctcgtaagg
     3481 gtacttctct tgcaattggt taatacccag attctcagct gtcaccacca atttcttcat
     3541 catctccgtt ataggtcttc ttgacaagac aagcggaagc aagatattgt ccttaacaga
     3601 cagagtatct agcaagttaa agtcttggaa gacaaatcct agcttttcac gccggaaact
     3661 agaagcctgt gaatttttaa tagttgcggt gtcagttcca ttcaagtaaa cctgcccacg
     3721 agttggttta tccaacatag ctagaatatt gagaagagtt gatttaccag aaccagactc
     3781 acccatgatg gcaacgtagt cacccttttc tacggtaaag tgaatatcct tgagggcctc
     3841 tacttggttg ccctgaaaac gtgttttata aattttttga acgtgtttta catctaaaag
     3901 tgtcatgaga atctcctttc ttaatatccc atcaaatgaa atgtagcttg catcatagtg
     3961 catcccagct gaacgcgctc aatttctttg tgacttggtt ttctagtttt cttatgtaag
     4021 atttgtttca tgatgttact cctttgttct ttatgagtct agtttacatc aaaaaaagac
     4081 cgcttgccat aacctaacct tacaaaagag actttaatct tacatttttg taagattggg
     4141 tgaaatataa gcaaaattac ttagaaaatg atttgatttc ctatttttaa agttgtaaaa
     4201 tataagtgtg aaaaagctta ctttgaaaag gaggaagcgg gaactaacat tttagtaagc
     4261 ttacctagat aacataccat tcaatttaac acttgcaagt cgttcaacct ataaggaaag
     4321 aaggtgcgct ttaatgaaac acaaagaaca tatactcatc ggacttctct atctgctctc
     4381 tccattcatc ggtcagctct tagttgaaca cacacacttt atcagtacag aatttaccgg
     4441 cactgcttat gttatttgct ggttatcagt tgttatcagc atccaccatt tttccaaaaa
     4501 tgtattgtct caacagcaaa aatagattaa aaaactgcgt ttaaattact gcttttatag
     4561 taaaatgaaa taagaacagg acaaatcgat caggacagtc aaatcgattt ctaacaattt
     4621 tttagaagta taggtgtcct attctagttc caatccacta tagtttgtgg atgaagctgg
     4681 cgaaatatta ttaatactat tcaaaaatca aagtgcaaac tatatcaact tcacattgcc
     4741 gactaaatta cagcctgcgg ctggcttcct agtttgcact ttgattttta ttgagtataa
     4801 aagtaaaaaa gctttaaatc aagcagttta gactattgat ttaaagcttt ttgcgtattc
     4861 caaatctttt gtaaaacctc accaaaacca gccttcatta tcgtcaatgg cttgagcttc
     4921 tttgcgttta cgtgggcctg ttccgtacat ttctgcttcg atttcttcct tggttggcat
     4981 gatagaagct aggatgatat agataatcac cccaagtcca aagtttgcga ctgtaaaaat
     5041 ggcaaacaga aaacgtacaa gggtaacatc aaagttccac ttgtctgaca gacctgctaa
     5101 aactcctgaa atcatgcgat ttcgtctcat tttataaaac ttactgttca tgatatttcc
     5161 tctttctgct aggttagaca tagtatatca cggataggct cggctttcat cagtcctcag
     5221 gctgatttac taatagcaac tttcctcgac aaagtccaca gcgatagcgt ttggtatcaa
     5281 tcctacgctt acgctgataa gtttgctggc aggattggca acgatagagc ttgattggct
     5341 tggagttact attgggcaag gatggtacaa accgtaatcc atccactgct ttcaacagtt
     5401 ccttaaaatc ccgatccttg tgttgatagc ctttcccttg aaaatagagg tgataatgac
     5461 agagttcatg tcggacaatt ttcctaaaaa cgtccaaccc cagttcctga taaaccttgg
     5521 gattaaaatc caaatgccca tctttgggga aaaatcgccc acctgtcgaa cgtagacgcc
     5581 tattccactg gacatgatgg ataaaaggtc tgccgaagtc ttctagtgaa acctgcttga
     5641 cgtaatcagt cagtttcatt tggagctagg agagacagat taactttttc acgttcagta
     5701 tcaattttct taacccaaac gctcaccaaa tctccaactg ccaccacttg actagggtgt
     5761 ttgataaact tgcgactcat atgggaaata tggatgagac cgtcctcatg aattccgata
     5821 tcaacaaaag caccgaaatc aacaacgtta cgcaccactc cttctagctt ttgtccaacc
     5881 actaagtcct tgatatctag gacatcttga cggagaactg gagcatcaaa ggaatcacgg
     5941 aaatctcgac ctggtttgag aagatctgca atgatatctt taagagtttc tggaccaagg
     6001 tctaactctt gcgccatttc cttgactgaa agcgacttga gtttgctttg ggcctcttca
     6061 ttcagatcct taatgtccaa gcatttgaag agttccttaa cggcagcgta attttctggg
     6121 tgaactcctg tattatcaag gatattgcta ctttcaggga tacggaggaa accagcagcc
     6181 tgttcaaaag ccttggcccc caaacgagga actttcttga tttgggagcg tgaagtgatt
     6241 tttccctctt cctcacggta tttgacgata ttttcagaaa tggttttatt gagtccagct
     6301 acgtgtgaaa gaagagctgg gctagctgta ttgacattga caccaacttg gttaaccact
     6361 gtatcgacaa caaagtccag actctcagat agtttcttct gactgacatc gtgttggtat
     6421 tgaccgacac caattgactt aggatcgatt ttgaccaatt ccgcaagagg atcttgcaaa
     6481 cgacgggcga tagaaatggc agagcgtttt tcaacggtca agtctggaaa ctcctgacga
     6541 gcaagttcgc tggcagaata gacagaagca ccactttcat taacgataac atagctgact
     6601 tcagggaaat ctttcagaac ttccgctaca aaagcttcac tttcactact ggccgttcca
     6661 tttccaatgg caataatctc tacaccgtat tgaccaatta aatctgctaa atctttcttg
     6721 gcttcttcga tttgacgagc tgatgctggt ttaacaggat aaataacctg agttgtcagc
     6781 atttttcctg ttgcatccac gacagctagc ttggcacctg tacgaaaggc tgggtcaaac
     6841 ccaagaacca cgcgcccttt cagtggagca accaagagga gattgcgtag attgtcagaa
     6901 aagagttgga tagctccttc ttctgctttt tcagtcaatt ctgtccgaat acgacgctcg
     6961 atagcaggca agaccttctt cttaacagat tgctgaacca cttcatcaat ataggcattt
     7021 tttaccttga aacgagcagc aaagaaggca agaatacggt ccgtcgcatg ttcaaagtcg
     7081 accttcaaga cacctagctt ctccccgcga ttgagagcca aggtacgata accctgcatg
     7141 ttcccaactg tctcagaaaa atcataataa atctgaaaaa cctgcttttc atcaagactt
     7201 tcatccttga cttgagaagt gagtttagag tgtctcagca cttcctgata agtcatggaa
     7261 cgcaaggtca catcttccga taaggcttcg accaaaatat caactgcacc agacaaggct
     7321 tccttaccag tcgcaaatcc ttcacagaca aacttctcag cctcttgttc taagtcagct
     7381 acattctgca aaatcaagcg agcaagtggg aagaggccag cttcacgggc aatgttcgcc
     7441 ttggttcgac gtttttcctt gtatggaaga tagagttctt ctacgtctgc tagtttttcg
     7501 gcttctaaga tagcttcttc caattcctta gtcagcttgc cttgttcttg aattttagct
     7561 aaaacaactt ccttacggtc atttagattt gtcagacttt tatccaagtc aataatggcc
     7621 ttaatcgcca cctcatccag actaccagtc atgtccttac gataacgcgc gataaaggga
     7681 atagtcgccc cttcagctgt caaacttaga acggtatcaa tttgctttaa agtcactccc
     7741 aaatcttggg agattttttc atatttttta tccatgaatc tattatacca caagctggaa
     7801 ggctttctcc aatccttttc atactcaata aaaatcaaaa agcaaactag gaagctagcc
     7861 gcaggc
//
LOCUS       DAVKDZ010000087         7717 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000087 DAVKDZ010000000
VERSION     DAVKDZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.088"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            153..452
                     /locus_tag="VWZ30_001850"
     CDS             153..452
                     /locus_tag="VWZ30_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9335530.1"
                     /translation="MKTKKHRLLALVLISSFTLLGAASTAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSIVSRWDGHSDKGEAPAGKTSYAWIWTKWGEQVAFYCDYD"
     gene            535..2556
                     /locus_tag="VWZ30_001851"
     CDS             535..2556
                     /locus_tag="VWZ30_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9335531.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            2558..3199
                     /locus_tag="VWZ30_001852"
     CDS             2558..3199
                     /locus_tag="VWZ30_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9335532.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            3303..4655
                     /locus_tag="VWZ30_001853"
     CDS             3303..4655
                     /locus_tag="VWZ30_001853"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU9335533.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGTIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAVNEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            4659..5717
                     /locus_tag="VWZ30_001854"
     CDS             4659..5717
                     /locus_tag="VWZ30_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU9335534.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLAVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQLENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            5727..6926
                     /gene="divIB"
                     /locus_tag="VWZ30_001855"
     CDS             5727..6926
                     /gene="divIB"
                     /locus_tag="VWZ30_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEU9335535.1"
                     /translation="MSKDKKNEDKETLEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
BASE COUNT         2393 a         1327 c         1682 g         2315 t
ORIGIN      
        1 taaaatatct aaaaattcga ttttttcctt gacaacaaat aataaaaatg ttattcttat
       61 cacataagaa agcggtttca acaaaagaga gaggagggtt tattttgttg cattgttgct
      121 caaggtgtgc tttttttgaa agaaaaaata aaatgaaaac gaaaaaacat agattacttg
      181 ctctagttct tatttcaagt tttacattat tgggagctgc atcaactgct gtacaatatc
      241 cagatggagg agtatggaca tatggagaag gttcaggagg tggttgggct ttttcaaatt
      301 actatcatgg taaaaaatat cattattcat ccatcgtgag cagatgggat ggtcattctg
      361 ataaaggaga agctcctgct ggaaaaacct cctatgcatg gatttggact aaatggggag
      421 aacaagtagc attttactgt gattatgact aatcgttcat tgatttaaat cgttcccctt
      481 gtaagaaatt acagggggaa ttttgtttac aaggagtcta ccaaggagtc tactatgaag
      541 aaactattca tattattatc aacttttttt ctcagcttct tccttgcttg gattattgtc
      601 ttacgtgcgc cacaatattt atatgcaagc tatgattccg tttctttact tcgtgtcaaa
      661 aaagatactc aggaaccgac gcgtgaggta tttgaacagg aattggagaa ttttgcaaac
      721 tcagaacaga gtttaatagc tagaagaatt gtagagccga gtaaggatgg gacgactcac
      781 tttacttatg caacttatgg tcagggaact ttaccaaaag aattccaaga agctagccaa
      841 gaaagtcgtg aacgtagtga tccgctaaat agttatctcc ttttgtcagg ctccttgacg
      901 aaagaaaagc ttgccgataa attaggagat ttgggttata aagcaagtgc tgaccgaaag
      961 ataccgccct attttcttgc ttttcgaata ttactaaatc cccttatttt aattagttta
     1021 gcaatatttg gcttatcttt ctttgctctg gtgattatta ctcggattaa ggaaatgaga
     1081 gcagcaggta taaaactctt ttctggtcag actctcttat ccatcatggg gcattcttta
     1141 tctactgata tcaaatggct ccttctatca gccctccttt ccttcctagg tgggggagtc
     1201 gttcttttta gtcaaggttt gttttatcct atcttgttag ccacctatgg ttttgggatt
     1261 agtttctatc tgttgttttt attggcgatt tcaattttac taatgctcct ttacctaatg
     1321 agtttgagtt acaaagcatt agttcccgtt attaagggga gattacccct taaacgcctg
     1381 atgattttaa ccctattgtg tcagttggtt gctgtcttta cagtaggata cgctgttaag
     1441 acaggtttga cgtcttacca acgattgaaa gaacttgaaa tttcaaaaca agcatggcag
     1501 gatagagcag actattatca aatttctttt ggcttaggtg atagaggaaa agatacagaa
     1561 aatcagagca agtggtatgc ctttgccaag gaagcaatcg aagaagaaca agctctttat
     1621 gtaaaggata atctgctcca ttttgccaat ccacaaggaa aaaatgaaca gggagagaca
     1681 ctggatacct atagtccaga tgctaatacg ctctatgtta gtcccagtta tttggacaag
     1741 gaaaaggtcg tggtagatgc tgagaccaaa cagaagttag cccatctcca aaaaggtgag
     1801 tttatcctct tgctcccaga acatttgcgc tctcgagaag cagaacttaa gaaagttttt
     1861 gaagaaagat tgagttatta tggaaaatct ggtgaggagg caagtgctcc tttggattat
     1921 gagatgaaag cgcacgttag ttatctttca atgggagaaa agcggtttgt ttataataac
     1981 ggtgagaatc ccgtatccac tcagtatttg actgatccga ttttagtagt attcacgccg
     2041 acttctacag gtgatagttt tatatcctta tctagttggt ctatcaatgc tggaaaacaa
     2101 ctctttatca aaggatatga gagtgggcta gaactcttga agaaagctgg aatttatgag
     2161 caagtatcct atcttaaaga aggaagaagt gtttatctaa ctcgttataa tgaagttcaa
     2221 actgaaacag caactttaat cttaggagct attgttggaa tagccagttc cttgttactc
     2281 ttttattctg tcaatcttct atatttcgag caattccgcc gagatatctt gattaaacga
     2341 atttcaggtt tacgattttt tgaaacacat gctcagtata tggttagcca atttgccagt
     2401 tttgtatttg gtgctagtct ctttatttta agcagtcgag acttggtgat tggcttgctc
     2461 actttattgg tctttctagc tagtgcagtt ttgacgcttt accgtcaagc gcagaaagaa
     2521 tctcgtgttt ctatgacaat tatgaaagga aaataggatg attgaactaa agaatatatc
     2581 taaaaaattt ggaagccgtc agctattttc agatacgaat cttcattttg aaggtgggaa
     2641 aatttatgcc ttaatcggta caagtggctg tggtaagaca acactcttga atatgattgg
     2701 acgattagag ccatatgaca aagggcaaat catatatgat ggcacttctc ttaaggacat
     2761 taagccttct gttttcttta gagattactt aggatactta tttcaagatt ttggcttaat
     2821 tgaaagccaa accgtcaaag agaatctcaa tctgggttta gttggtaaaa agttgaagga
     2881 aaaagagaaa atctctttga tgaaacaagc tctaaaccgt gtaaacctct cttatttgga
     2941 tttgaagcaa cctatatttg agttatcagg aggagaagca caacgtgttg cactagcgaa
     3001 gataatttta aaggatccac ctttgatcct tgcagatgaa ccaaccgctt ccttagaccc
     3061 caaaaactct gaggaattac tttccatcct agaatcttta aaaaatccga atcggaccat
     3121 tattattgcg acccataatc ctctgatttg ggagcaagtg gatcaggtca ttcgagttac
     3181 cgatttatca catagatgat atggtaagat tcagttagaa gaaagagtca caaacacact
     3241 ttgtggcttt tttatttcca taaaaatggt aaaatagtag gagtagaaat ggagttcgag
     3301 acatgaaagt aatagatcaa tttaaaaata agaaagtcct tgttttaggt ttggccaagt
     3361 ctggtgaatc tgcagctcgt ttgttggaca agctaggtac cattgtgaca gtaaatgatg
     3421 ggaaaccttt cgaggacaat ccagctgccc aaagtttgct ggaagaaggg atcaaggtca
     3481 ttacaggtgg ccatcctttg gaactcttgg atgaagagtt tgcccttatg gtgaaaaatc
     3541 caggtatccc ctacaacaat cccatgattg aaaaggcttt ggccaaggga attccagtct
     3601 tgactgaggt ggaattggct tatttgattt cagaagcacc gattattggt atcacaggat
     3661 cgaacggtaa gacaaccaca acgactatga ttggggaagt tttgactgct gctgggcaac
     3721 atggtctttt atcagggaat atcggctatc ctgccagtca ggttgctcaa atagcatcag
     3781 ataaggacac gcttgttatg gaactttctt ctttccaact catgggtgtt caagaattcc
     3841 atccagagat tgcggttatt accaacctca tgccaactca tatcgattat catggattat
     3901 ttgaggaata tgtagcagcc aagtggaata tccagaacaa gatgacagca gctgatttcc
     3961 ttgtcttgaa ctttaatcaa gacttggcaa aagacttggc ttccaagaca gaagccactg
     4021 ttgtaccatt ttcaacactt gaaaaggttg atggagctta tctggaagat ggtcaactct
     4081 acttccgtgg tgaagtagtc atggcagtga atgaaatcgg tgttccaggt agccacaatg
     4141 tggaaaatgc ccttgcgact attgctgtag ccaagcttcg tggtgtggac aatcaaacca
     4201 tcaaggaaac tctttcagcc ttcggtggtg tcaaacaccg tctccagttt gtggatgaca
     4261 tcaagggtgt taaattctat aacgacagta aatcaactaa tatcttggct actcaaaaag
     4321 ccttatcagg atttgacaac agcaaggtcg tcttgattgc aggtggtttg gaccgtggca
     4381 atgagtttga cgaattggtg ccagacatta ctggactcaa gaagatggtc atcctgggtc
     4441 aatctgcaga acgtgtcaaa cgggcagcag acaaggctgg tgtcgcttat gtggaggcga
     4501 cagatattgc agatgcgacc cgcaaggcct atgaacttgc gactcaagga gatgtggttc
     4561 ttcttagtcc tgccaatgcc agctgggata tgtatgctaa ctttgaagta cgtggcgacc
     4621 tctttatcga cacagtagcg gagttaaaag aataaaatat gaaaaaaatt gtctttacag
     4681 gtggggggac ggttggacac gttaccctca atcttttgtt aatgcccaag ttcatcgaag
     4741 atggttggga agtccactat atcggggaca agcgtggtat cgaacaccaa gaaatcctta
     4801 agtcaggttt ggctgtcact ttccactcca ttgcgactgg gaaattgcgt cgctatttct
     4861 cttggcaaaa tatgctggac gtcttcaaag ttggctgggg aatcgtccaa tcgctcttta
     4921 tcatgttgcg acttcgtcca cagacccttt tttcaaaggg aggctttgtc tcagtaccgc
     4981 ctgttatcgc tgcgcgtgtg tcaggagtgc ctgtctttat tcacgaatct gacctgtcta
     5041 tgggcttggc caataaaatc gcctataaat ttgcgactaa gatgtattca acctttgagc
     5101 aagcttcgag tttgtctaag gttgagcatg tgggagcagt gaccaaggtt tcagatcaaa
     5161 aaaatccaga accagatgaa ttggtggata ttcaaagcca ctttaatcat aaattgccga
     5221 ctgtattgtt tgttggcggt tctgcaggtg ctcgtgtctt taaccaattg gtgacagacc
     5281 ataagaaaga actaacagag cgctacaata ttatcaatct aactggagat tctagcctga
     5341 atgagttgag ccaaaatctt tttcgtgttg actatgtgac cgatctctat caacccttga
     5401 tggaattggc tgatattgtt gtgacacgag gtggtgccaa tacgattttt gagctcttgg
     5461 cgatagcaaa attgcatgtc attgtgccgc ttggtcgtga agctagtcgt ggtgaccagc
     5521 ttgaaaatgc agcttacttt gtaaaaaaag gctatgcaga agaccttcaa gaaagcgatt
     5581 tgaccttgga tagtttggaa gagaagcttt ctcacttact aagtcacaag gaagattacc
     5641 aagctaagat gaaagcttct aaggaattga aatctctagc agatttttat caattgttga
     5701 aaaaagattt atcataagga aagtaaatgt caaaagataa gaaaaatgag gacaaagaaa
     5761 ccctcgaaga attgaaagag ttatcagaat ggcagaaacg aaaccaagaa tatctaaaaa
     5821 agaaggctga agaagaggcg gctctagctg aggagaagga aaaggaaaga caagctcgaa
     5881 tgggagaaga atctgagaag tcagaggata aacaggacca ggagagtgaa acagaccagg
     5941 aagattcaga atcagctaag gaagagtctg aagaaaaagt agcatcctca gaggctgaca
     6001 aagagaaaga agagaaagaa gaaccagagt ctaaagagaa ggaggaacag gataaaaagc
     6061 ttgctaaaaa ggctacaaag gaaaaaccag ccaaagcaaa gattcctggt atccatatct
     6121 tgcgagcctt cacgatttta tttccaagtc tgcttttatt gattgtctct gcctacttgc
     6181 tcagtcctta tgcgaccatg aaagatattc gtgttgaggg aacggtgcaa actacagctg
     6241 atgatattcg acaggcttca ggcattcagg attcggatta tacgattaac cttctgctag
     6301 acaaggcaaa atatgaaaag cagattaagt ctaactattg ggttgaatca gctcaacttg
     6361 tctatcaatt tccaactaag ttcactatta aggtcaagga atatgatatt gtggcctact
     6421 atatttctgg tgaaaatcat tatcctattc tttccagtgg tcagcttgag actagttctg
     6481 tgagtctgaa cagtttacca gaaacttatt tatcagttct ctttaatgat agtgaacaaa
     6541 tcaaggtttt tgtctcagaa cttgctcaaa ttagcccaga actcaaggcg gctatccaaa
     6601 aggtggaatt agccccaagc aaggtgacat ccgatttaat tcgattgacc atgaatgatt
     6661 cggacgaagt cttggttcct ctatctgaaa tgagtaagaa actgccatat tacagtaaga
     6721 ttaagccaca attgtcagaa ccgagtgtgg tcgacatgga agctggaatt tacagttaca
     6781 ctgtggcgga taaattgatt atggaggctg aggaaaaagc caaacaagag gccaaggaag
     6841 ctgagaaaaa acaagaagaa gaacagaaaa aacaagagga agagagcaat cgaaaccaaa
     6901 cgacccagcg ctcatcgcgt cgctaggttt actttttctc ttatagttat ttagtgacca
     6961 tgtttttacg tttaatattt gacatctgtt tttctttatg ttatatttgc aatgtaatcg
     7021 attacaaaaa aattttttat gaagaaggta acacatatgt cagatgaagt attcttattt
     7081 gaagaaattg aagaaatcgt agcccctaca gatggtgaat tttggggggg agttttactt
     7141 ggaactgggg tagttctctt aattggagta gcctgttgtt aaaaagatag ggagtgataa
     7201 tcatgcaaga taacttttta tttgaggaaa ttgaagaaat ttcagtacca gttagtgatt
     7261 tttcagctgg acttgcaaca ggtatcggat ttggtttagc aatccttgct cttgctggtt
     7321 gttgaagttt gttcacttac taatatcaag ctttttcaat ttcattttag acagtcattt
     7381 gaattttccg tattagtctt gcagcaagag attaatagaa ttagtcatta ttttattgat
     7441 tgcggactga gggactagag tatgttttac ttagcccctc ttttatttat taaatgttag
     7501 gtttgttatg agaattgttg ataagattaa gatattacct actccttatg agggacagta
     7561 tcatctatat ataccatcca gtaagaaaca tgtattagtt gggaaacagg aaaaaaaatg
     7621 gttagagcaa ctaataggtc aagaatttac catatcggac ttattagtgt tagtagggaa
     7681 gaaatattat ttaagatttt atattgttct tagtaaa
//
LOCUS       DAVKDZ010000088         7441 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000088 DAVKDZ010000000
VERSION     DAVKDZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7441
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.089"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(143..1558)
                     /locus_tag="VWZ30_001856"
     CDS             complement(143..1558)
                     /locus_tag="VWZ30_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9335536.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLLGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWVDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1571..3409)
                     /locus_tag="VWZ30_001857"
     CDS             complement(1571..3409)
                     /locus_tag="VWZ30_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9335537.1"
                     /translation="MSYKDKVQKIFDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQSTLVLLVSSCIALVVVGPIGVIVGQGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALFAGITEPAVYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3427..4266)
                     /locus_tag="VWZ30_001858"
     CDS             complement(3427..4266)
                     /locus_tag="VWZ30_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU9335538.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGVIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            complement(4565..7245)
                     /locus_tag="VWZ30_001859"
                     /pseudo
     CDS             complement(4565..7245)
                     /locus_tag="VWZ30_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT         2444 a         1561 c         1028 g         2408 t
ORIGIN      
        1 cggctctctg taacaaaatt aaattaatta atccccctat aaaataaaga ggtttagaat
       61 ttgatgtctt ccaaacctct ttatttcata tttaatgaga tgttgcctta accgtgataa
      121 tatctaatca tcaataaaca aactatttga ataaggattc tccatttgat tcaatcactc
      181 ctttatacca agtaaaggac attttcttat atcgattgaa tgtgccattt ccatcatcat
      241 ttcgatcaac ataaatgaga ccgtaccttt tagaaagttg tgcagtggac atagaaacac
      301 agtcaataca tccccaagac gtatagccca taatttcaac accatcctgt agagcttcag
      361 caacttgcaa taaatgttct ttcatatact gaattctata atcatcttgg acggttaagt
      421 tattaagttc atcttttatt agttgatctt tagcacctaa tccattttct actataaata
      481 atgggatttg ataacggtca taatatctat ttaaaattat acgtagtcca attggatcaa
      541 tttgccatcc ccactctgaa gactctaaat aaggatttac taaaccacca ataatattcc
      601 cttctcctga attatactgt gttggaagag cagattgagt cacactcatg taatagctaa
      661 aggataaaaa atctacagta taatttttta ataactctgc atcttcagct gcaaactcta
      721 tgttaatgtc attttcctta aaatatcttt ttgcataatt cggataataa cctctaacat
      781 gcacatctga aaatagataa tttagattct catactcatg agtcgcccat acatcttttg
      841 gatttggagt cattggataa gctggcatag ctaataccat acatcccacc ttaaactctg
      901 aattaatctc acgagcaatt tttgtaacca aacttgaggc gactaattca tgatgtatag
      961 cttgatataa ttcttgtttc gaaagattct ccttaggtat atctattcct ccactagtaa
     1021 atggtaattc caaaacagag tttacttcgt taaatgtaag ccaatattta actttatctt
     1081 tatacctttc taaaactgtt cgagcaaatt tttcataaaa atgaatcatt ctcctatcaa
     1141 cccatccatg atattttctt gctaaatata atggagtctc atagtgtgaa agagttacaa
     1201 gtggctctat cccgtgagca tgtagttcat caaacaattc atcataatat ttcaaaccag
     1261 cttcgttagg ttcttcttca tctccttttg gaaaaattct actccatgca atagaagtac
     1321 gaaaaacatt aaagcccatt tcagaaaaca aggatatatc ttccttatat ttatgataaa
     1381 aatcaatacc taacaatttt aagttatctt ctgtaggatt ttctgttgct tctcctaatc
     1441 cacctttggg taacacatcc tgaactgata agcccttacc atcttcatta tatgctccct
     1501 ctacttgatt agctgcaaca gctccacccc aaagaaaatc atctggaaaa atggtcataa
     1561 ctttcctcca ttataatatt accagtaatt ccttagaatg ctcgattgtc tgattattag
     1621 gtaatactaa tacatctaga aaatcattgg tattcgttac aattactggt gtaactgttt
     1681 cgtagccttt agtcttgatt aaattcaagt ccatttcaaa aatcaactga tttttgaaaa
     1741 ctctgtctcc ttcttctaca tgactaataa aaccttgacc ttttagctca acagtatcta
     1801 atccaatatg aattagtaac tcaacaccct catcactctt caatccaatt gcgtgcttag
     1861 tcggaaaaat atttgtaatt ttcccatcaa atggtgcata aaccttacct tcacttggga
     1921 taatcgctac cccgtctcca attagtttat ctgaaaatgt tttatcctgg acatcgctta
     1981 acggaatgat ttctcctgat ataggagaaa atatcatttt tttatttgaa actccagctt
     2041 taacttctaa attgctagaa ctctcttctt catcgattcc aaatatataa gctaatacaa
     2101 aggtaataac aaccgaaata accgccacaa ttaaagcatt tacaatattt gatggtacat
     2161 cagaataaat aaattgaggc aacgctatca aagatgggac agcaaataga tacgctttaa
     2221 cactagtaag acctgcaaat aatcccgcta atccaccacc aatcatagct gcataaagcg
     2281 gttttttata ttttaaagtc acaccatata ctgcaggttc ggtaattcct gcaaataagg
     2341 ctgagaaacc tgctgcaaaa gcaatttgtt ttgtattatt atttttactc tttaatgcaa
     2401 cagccatcga agcagcccct tgagctaagt ttgaccctaa cattgctgga agaattaata
     2461 cgtctggagt agcaatagat gccgccaaaa aaataggtgc aaaagcccaa tgcattccag
     2521 tcataacaat aaatggcata atagcaccaa gaatagctaa tgtaagccat ccagctacac
     2581 catacatttg cccaactaga tttgataatc cttgaccaac aattactcca ataggtccga
     2641 ctacaactaa ggcaatacag cttgatacta ataatactag cgtagattgc aaaaaactct
     2701 tagtaacagc tggtgttaat ttagcaatca ttttttcaat atatttcatc aaccaaacca
     2761 taataagaat tggaacgact gacgaaccat aactagctgg tgtcacaggt acaccaaata
     2821 aactaagagg attccctgat tgcaccattt gaacaaaatt tggatggaga agtacacctg
     2881 ctacagacat agctaatgta gatgttactt ttaatttttg tgatgcagaa taagctaata
     2941 acagcggtaa gaaataatat ggagcatccc caaaaaatgt caaaaaagca atagtctgag
     3001 aatctgattg caatatacca agcattggta aaataattac caagactttc agcatacctc
     3061 cccctaacat tgctggaatg attggagtca tggaaccagc gatatactca atgattcttt
     3121 ctaaaatatt ccctttgtgc ccttgaacaa ctgaatcgga ttcaaaattg ccaagtttaa
     3181 cgaattcttt ataataatta gctacatcat taccaagtat aatttgatat tgtccattct
     3241 ttttcataat acctattaca cctggtatct tcttcacatc atcatcattg actaaatttt
     3301 catcttttaa ttctaatctt aaacgtgtta cacaatgggt aactctattg acattttttt
     3361 cacctccaat tacatcgaag attttttgta ccttatcttt ataactcatg gtattctcct
     3421 attctattaa tctaaatttt ttgttaagcg acgaatatga gccatcaaat aaactaattc
     3481 actagaagtc agcaaataat tgtactcagt ttgtataaac attgccacct gttcaccaca
     3541 ttcatattct ctaggatatt tatttttcat taatgctaac aagtcttcat catcatcgtc
     3601 acaataagtt gtattttcaa ccaagcgatg ggcaaataat tttatatgag ttatgaatct
     3661 ataataatca atagaatctt ggttaaactc agtacaatag aaatcttgaa tgattttctc
     3721 aattcccata attatttcag tgattttata cgactcttga aaattatttt ctaaattagc
     3781 attaacaaaa tgtagcgcta tgaatgcagc ttcgtcaatt ggaagttcaa tacctaactc
     3841 tttttttatt atttgtaaag cccttttccc aagctcaaat tcttctttat aatatttcga
     3901 gatttcccag cgtaggggat tcgaaataat aactccttct ttataacgtt ctatactcga
     3961 agaaatatga tccgttagat ttatatagat aatctcatca aaattattac ctaatgttat
     4021 tttacctaga ttaattattt tttcggtgat gtacaccaac tccaaaggaa cgttaataaa
     4081 catctctgta aaacgggtca tattatctct atttttgaga aaaaaacttt tctcaatatc
     4141 aattggattt actttatctc cagtttttct tccaaaagct attccccttc caaaaagaag
     4201 aatatcgata tcttttttac tttgcgcaat tacggcattg tggtttagta ttcgttttat
     4261 gatcataaat ttccctttta aaataaaagg aacctactta aaaacatcaa gaaacttctt
     4321 ccgatgtgat taaataggtt cctgctcact tgtattagtg ataacatcct tttatttcaa
     4381 tttttaatgg tgacatgaag cttaaatcat taataactct gtaaccattt tcattttatg
     4441 atatttcaat ctatttgtca agtacttgta gaaatttttt tattttgtgt gattcgaatg
     4501 tgataatggc aacagtctca ctaaagaaat gaaaatcata catagaaatt tttaaaaatt
     4561 ttctctatcg taaacctgcc actagttcag aagctatact agctgattcc aaacgtagta
     4621 tggtcatttc atctaccttc cctaataatg gcattgcgcc ataccaaaca atagtcttat
     4681 tcaccaaaag aataggcatg actattttta tttgagtaaa agatacttta attttcttgc
     4741 tcttcaactg gtctagccat gctttatttg caaccgtctc tggtatacat atctcaattt
     4801 gactaatcct aaattctttt agaaaattca tcagttttgt ctggtgcaca taaggtaaaa
     4861 taagcagaca ttcctgtctt tcccctgcta agtcctctct caacaccttc tcatatctac
     4921 tatcaacata aacgaattgt ttctcctcac cctcgatgac acgataatcc atctttcgat
     4981 aagctacttg tcgtttctga aacatctttt ctaaataagg aacatgaata tccacataat
     5041 cgaaaatacg caccaaagac ttatccttgt agtttctatg aatccgacct gcatactgaa
     5101 ttaaattatt tttccaagaa aagggtgctg ccaagataag cgtgtccaac tgaggtaagt
     5161 caaaaccttc gccaatgtat tttccagtag acaacaaaac aaacccttta tctaactgtt
     5221 ctaacgtctc cagtaaactc gttctttctc ggactttggt ttttccgcta ataatgtaac
     5281 agtcatcaac ctctttctct ttcaataact tttcaaagac atctatctgt tgaattcgat
     5341 taactaaaac caagatattc cgtccttctg ccacttgggc tagaatatcc ttgagaatca
     5401 actgattcct cgctgagtca gtagcaatcc aatcactaag ctgtataaaa ttacttgctt
     5461 tggtcttttc aatctccaaa tgaccaaaag aagtgaatct taattgcaat tgccgtttaa
     5521 aatccgtttc cctcttatca gcagtatgga gtatctcacc aattctctga aaaacaatag
     5581 gctcatgacc attcttacgc tcaggcgtag ccgtcaaacc gtaaagatac ttccctctaa
     5641 actgagcaac aactttttca aacatcaagg cagagacatg atgacactca tccacaatca
     5701 tcatctcata ctcatccaaa agactttgac tattttccaa cttaaataga gattgaatca
     5761 taacgacatc aaccagttta ctaagccatt tcttagtccc accgtactgt ccaacatagc
     5821 cgattacctt ttcacgacct gatgccgtat aacggatagc ctcctcctct tcgaaagtca
     5881 aaaagcagtt taagcgatct agccattggt ctaagagttg cttattatgg actagaataa
     5941 ttgtttttgt tttccgttca gagataagag cagcacctaa aacggtcttc ccaaaaccag
     6001 tctccgcatg aagtaaacca ttttctttag aagtcatatc tgacagggct aactcttgct
     6061 caaaagtgag ttctccctta aattccacgc taatagacct ttgtaccttt ctcctatctt
     6121 ccacagatac ctgcttaaat ttatcttgca atggatacag caaacctctt ggcaaccata
     6181 aataatgatc ggattctcca aataaataca ttctctcagg aatttgatag gttggctgtc
     6241 gcatagcctg ctttaaataa aattcgggat tagaaaatga agccatattt ttcaagaaaa
     6301 acagtgtctt gggtgtcaca gacgattttt caagttggat catattggat aagacgatct
     6361 tcaactcctt gtccaattct tgcttgccta actcttcttg gattaacagt gccactttag
     6421 cagttgaaac cctctgaatt tcttgtagat acctccattg gtcttcataa ggctgaaact
     6481 gttcatccac aaagaccgtt cgcccttgat ggtaagcttc tccttgaaaa ggcaaggcaa
     6541 tcaaatttcc aaatcccccc ccttaggaag gacatcctga tttggaaaca tgcgatcaaa
     6601 agaatcaaag gacagttgca tactttcctg cattgccagt tctatcagtt tctttccaaa
     6661 caagcgagcc tctcgactcg gaatcgcttc ctcaaagaag aaccaaatat ggagtccgtg
     6721 acccgaacga gaaatctcta aatgggcttc catctggcgt tccctggcta ttcttcgaat
     6781 ggttaaacca gcttctttcc aatctccttc atcaaaatcc agtaccaaaa aatgacagct
     6841 atcatctaag tgcataggaa agataccgat agctgtctcc ccacgaaaat gagatttcaa
     6901 aacggaatcc gtcaatgtct ggaaactccg tttttcagat agcaactgct tccaaccata
     6961 atcataggaa ggaaaatact gaattttccc ttgatcattg ataaaactct tagcataaac
     7021 atcataacgg ccagcaaaca cagaggtaaa caattgtaat ttttcttgcg tcgtcaagcg
     7081 actactttga agttggaaca tctcctcgaa ctgagagcgt tccataacaa actgactctt
     7141 gtctttaaga cgtttacaca agaaaaactc actatcccca ttaaaagaag aaaagacatc
     7201 aagtacttct accacaattc catccaaaga cacaaaaaca gccataagag tcacctcctt
     7261 gattcctata ggctgattat aacaagaata gctgaaattg tacacgaaaa taaaatccga
     7321 ataacactca ttttgtatgc gaccaatagt ccatctcgct ccggaatgta cgaggtaaat
     7381 agagtttaaa cgagcagtct ataaaattca aaaaacgcat catatcaggt gtacatgaac
     7441 t
//
LOCUS       DAVKDZ010000089         7042 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000089 DAVKDZ010000000
VERSION     DAVKDZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.090"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..202
                     /locus_tag="VWZ30_001860"
     CDS             <1..202
                     /locus_tag="VWZ30_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9335539.1"
                     /translation="NNYSRVISGSAQPQLPITKLKKILLPLPPLALQNEFADFVTLVD
                     KSQFACEIAIKVWRNSLKFSII"
     gene            232..3582
                     /locus_tag="VWZ30_001861"
                     /pseudo
     CDS             232..3582
                     /locus_tag="VWZ30_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            3853..4008
                     /locus_tag="VWZ30_001862"
     CDS             3853..4008
                     /locus_tag="VWZ30_001862"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335540.1"
                     /translation="MKIKEQTRKLAAGCSKHGFEVVDETDEVSNHTYGKATLTRIEEI
                     FEEYKSS"
     gene            complement(4125..4595)
                     /locus_tag="VWZ30_001863"
     CDS             complement(4125..4595)
                     /locus_tag="VWZ30_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9335541.1"
                     /translation="MNKSEHRHQLIRALVTKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4612..6885)
                     /locus_tag="VWZ30_001864"
     CDS             complement(4612..6885)
                     /locus_tag="VWZ30_001864"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU9335542.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFNLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSENELTADIFYTVAFQLLGFAYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSSQDVIREVVYVESRVDTDQKGLPDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEQPQLNLVQPQGQAELVAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRRRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVIFTHGSQDWNVKPLHVYQIFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQAFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         2175 a         1192 c         1513 g         2162 t
ORIGIN      
        1 taataattat agtcgagtga tatcaggaag tgctcagcct cagttaccaa ttacaaaatt
       61 aaaaaaaata cttctccccc tccccccact agccctccaa aatgagttcg cagactttgt
      121 aaccctggtc gacaaatcac aatttgcttg tgagatagct ataaaagtgt ggagaaatag
      181 cttgaaattt agtataatat agctaaacta tttgtttaaa gtgagaaaaa aatgggaaat
      241 tttagctttc ttttaaaaaa tgacgaatat gaatcttttt caaaaccttg cattgaagct
      301 gaaaatatga ttgctacatc aactgtggct actgccttta tggcgcgtcg tgctttggag
      361 caggctgtcc attggatata tagtcacgat tcatatttag aagctcccta tcgtgctact
      421 ctatcttctt tagtatggga tgatgatttt agggatatcg tagattctga actccacaag
      481 cagatagttc tgttgattcg gtggggaaac catgctgctc atggtggtga aattaaggaa
      541 cgagaagcga ttttagcttt acatcatttg tatcagtttg ttaattttat cgattattgt
      601 tacagcaatg aatttgtgga gcgttatttt aatgagaagt gcttaccact ttcagcaaac
      661 atcaaatacc gagaaactcc acaatctatg ataaagttac aagacagttt accagaactg
      721 cctgattttc atgaacagat ggctgctcag tccgtagaag ttcaagagac ttatactgaa
      781 aaacgtgaga ctgcagcgca acggcaagat gtgcctttcc atattgaaca attatctgag
      841 gcagagacaa gaaagctctt tattgatatc gatctccgtt tagcaagatg gatatttgaa
      901 gaaaactgtc gtgttgagat agccgttgat ggtctcaagc acggttcagg aattggttac
      961 tgtgactatg tactttatgg taaaaatggg aaaattttag cgattgtaga ggctaaaaaa
     1021 gcctctgtca atccagaagt aggggaagta caggtcaaag aatatgctga agctcttgag
     1081 aaacatatcg gctatcagcc aatttgcttt attacaaatg ggttgaagca ctatatactt
     1141 gatggtccga accgacgcca gattgcaggc ttttactctc aagaagaatt gcaattagtg
     1201 atggatagac gtcatcttca aaaaccactt gaggatattt ctagtaaaat tagagacgat
     1261 atttccgggc gttactacca aaaacatgcg attgcaagcg tttgagaagc tttctctgat
     1321 catcgtagac aggcactttt ggttatggca actggggctg ggaaaactcg tacagcagtt
     1381 tctctagttg atatcttatc acgtcataac tgggtaaaaa acgttctctt cttagccgat
     1441 agaacttcct tggttaagca agtatatgat tcgtttagaa aattactccc agatctttcc
     1501 gtttgtaact tcttagaaga taaagaagga gctcaatcaa gtcgcatggt cttttcaact
     1561 tatccgacca tgattggagc gattagtggt caagaagaag taaatcaacg ccctttcact
     1621 gttgggcatt ttgaccttat cataattgac gaatctcacc gttctattta tcagaaatac
     1681 aagtccattt ttgattattt tgatgcaaga attgtaggct taacagctac tccgcgtcaa
     1741 gatttagata aaaacaccta tggattcttt aatttggaga atggggttcc aacatatgca
     1801 tatgatttgg aagaggctgt taaagacgga tatttagtag cctatcattc tatcgaaacc
     1861 aaactgaaac tacctacgga tggtctacat tatgatgatt tgtccgaaga agaaaaggaa
     1921 cattttgata gcaaatttga agacgatatc tgtgaaaaag atattgatgg gagtgtattt
     1981 aattccttta ttttcaataa aagtacagta gaaattgttt taaatgaact catgacaaga
     2041 ggaattcaga cagcctcggg tgatgaaatt ggtaaaacta ttatttttgc taaaaatcat
     2101 gatcatgcgg aatatatcag aggtattttt aacaaccgct atcctgaaaa agggagcgac
     2161 tatgctcagg tgattgatta tagtattaag cattatcaga ccttgattga tgattttaaa
     2221 attaaggaga agtatcctca aattgcgatt tctgtcgata tgttagatac aggtattgat
     2281 gtatcagagg ttgttaattt agtcttcttc aagaaagtac gctctaaaac taagttttgg
     2341 cagatgattg gtcgaggaac ccgtctatgt aaagatttat ttggacctga gcaggataag
     2401 gaaaacttct tggtatttga ttatggggac aattttgatt attttcgtgc agatccaaga
     2461 gatggagagg gtcgtcacat tgtttcgttg actcagcgtt tatttaatat caaagtggac
     2521 ttgattcgag aacttcaggg actccaatac caagaagatc agtttgcgag agcataccgt
     2581 cagcagcttg tctcggaact tcaaggtcgt atagagagct taaatgagtt ggacttcagg
     2641 gttcgtatgg ttttaggtac agtttatagc tataggaaat tggaaagttg gcagaatcta
     2701 actgctgtta caagtgaaac cattcaaaaa aatctctctc cgcttttatt tgatgaagat
     2761 aaagaagatg agatggcgag gagatttgat ttgtggttgc ttcatattca gttggggcaa
     2821 ctgacagcta aatcttccac tgttcatatt tcccaagtga tgaagacggc tagagctctt
     2881 tctgctattg gcaatatccc gcaggttttt gagcaggctg aaattatcag gaaagtacag
     2941 gagcctgaat tttggaaaga agttaacttg tctgatttgg aaaaaattcg tcttgctatt
     3001 cgagatttat tacagttttt ggataaaaca gaccgtaaac tctactatat taactttgaa
     3061 gatcgtatac tctccactgt tcacgagacc acagcatttt tgcaggtcaa cgatcttcgg
     3121 tcttacaatg aaaaagttga gcattatttg aaaactcatc tggatgagga gtccatttct
     3181 aagctatacc ataataaaaa gttgacatct gatgatatgc ttgcacttga aaaattgctc
     3241 tgggaaaaat taggtagtaa agcagactac caaagtcatt atgaaaataa ggcaattccg
     3301 agattggttc gtgagattat tggcttagat agagagtctg ccaatcgtat tttttctaaa
     3361 tttttgtcgg atgagaatct taatgccagg cagatttcat ttgtaaaatt gattgtagac
     3421 tacattgtag aaaatggttt tttagagacg aaagtgttaa cgcaagagcc gtttaaatct
     3481 tatggttctg ttcaactact cttccaacac caactaccag tacttcgtaa tattgttcaa
     3541 atcattgaac ttatcaataa tcgagctgga gaagcggctt aaattctaaa gtgattgcca
     3601 tgctgagact catttaaaat taaaaagagt agaaatttat gctatatatg agaagtttta
     3661 ttaggaagaa tgtcatcgtt ttcctagaat acagtatcag ttgttaagtg gttgataaat
     3721 ttcaaagtag atacttgtac cacgatgttt gttgatcgag ttattaacaa aagagctact
     3781 ttgattttaa agaaatagaa aacaaaaagc cgagcaagaa ttcaattgca ggagaaaatg
     3841 aaataatact caatgaaaat caaagagcaa actaggaaac tagctgcagg ctgctcaaaa
     3901 catggttttg aggttgtaga tgaaactgac gaagtcagta accataccta cggcaaggcg
     3961 acgttgacgc ggattgaaga gattttcgaa gagtataaat cttcctagga taaagcaaaa
     4021 cgcatagtat caagggtttt caacacttga tactatgcgt tttctgatgt taaagacttt
     4081 ctaccaggtt ttttaaaagc ataattgtta gttgtagtca tttattattc ttcaaagaaa
     4141 aatggtgggg cgaatttttt cagttcttca aagcactttt gagcagtatc tgcatcttca
     4201 cagatgataa gacagacatc attaccacaa agggtagcga tagcgtcagg gaagctcaaa
     4261 gtatcaatga tagaaccgaa ggattgagcc agtccaggaa gggtttttag taggacttgg
     4321 tgttgaactg ggcgcatcca gacaagggca tcttccatgt agagttcgag acgtttttcc
     4381 cattttgaga tggaaccatt gttaagaaca taataagcgc tatcttcttc gcggactttt
     4441 gataggttca tatttttgat gtcgcgtgag agggttgcct gggttacttg aatgtcgttc
     4501 tcagcaagaa gggcttgcaa ctcagcctgt gtatgaatct tgttttttgt gacaagagcg
     4561 cgtataagtt ggtggcggtg ttctgattta ttcataataa tgtaattcct tttagcaagg
     4621 taaggtaagc atggactgag cgaggtcgac agtcaagtgg tagtctgtat tgtcacggat
     4681 ggtgatttca aagtcagtag tatagaggac taaacggaga gtgtctcctt cttttagctt
     4741 gtaaatagtt ggctgcagtt caaattgaac gtccatccat tcatctgcag taatatcctc
     4801 tactaacagt aaatcatttc tattttgtaa attaaggtaa ccttttgtca cgactcgttg
     4861 tgcctctggt ctaaatggca attcacagag attttccaac atgtgatagc gaccgttgtc
     4921 aatggttcta gcacttaaaa tagctggata aggttgtagg tatttctttt gcccaaattc
     4981 tagcagttgg gcagataaga gccccttgtt tgtactggat ttgatacgaa gattgagctg
     5041 agcgcgaccg tttaggtgga gatctttagt cacaggaagg ttaatagtaa tctgattagc
     5101 tttcccttga tagagctctg tattgaaggc ttggtatgtc ttaccatagc gctcaaaatc
     5161 cttatctggg tactggtttt gaatagcttg ctcttcttga ccaagtgaga aggtttcaca
     5221 gttttcttgc ccaccgaagt tatcaagtga taaccaagtc tgtggagctg tattgtcctg
     5281 ccagataaca gtaggaagtt gaaagtctgt ttcctgtcct agtaatttct tggttaataa
     5341 ggcatttatg gactcacgga agtcaattga ttgccaattg ttcatgtaaa catgggcacc
     5401 attatggaaa aagagatgct tgtgtatatg agtaggaaga gcatggaaga tctggtaaac
     5461 atgaagtggt ttgacattcc aatcctgaga accatgagta aagataacct ctgcctttac
     5521 tttatgggca ttgagcagat aattgcggtc atgccaaaac tgattgtagt ccccagtttt
     5581 tcggtctagc tgagctttca ctttttctaa gtcagcttgg tgagcttcat tgccacggat
     5641 atagtcgcca gctaagagat tacgagaata ggttaactca gcaagggagt caaagtcctc
     5701 acctggataa ccacctgggc tagtcaccag accgttttca cggtagtagt tgtaccatga
     5761 tgaaattcct gcctcggcaa tgataacttc taaaccatcg actcctgtag tcgcaagacc
     5821 attggacatg gtacctagat aggaaagtcc tgttgtagca acttttccgt ttgaccaatc
     5881 agccttgact tgacgctggc gcgtgtgatc agtaaaggca cggcgacggc cgttaagcca
     5941 atcgatgaca tttttataag cctcgatttg ctggtagtct ccattagtca tgaaacctgt
     6001 cgagtctttg gtaccaacac ctgagacata gagattggca aagcctctcg gaaggaagta
     6061 gtcgtttagt gtatagctag agttgatgtg agttagcttt tcctcagcct ctgctacaag
     6121 ctcagcttga ccttggggtt ggacgagatt tagttgaggt tgctctagct caatcttgtg
     6181 aggaagctta acctcaagct cgccctccat cttgtagaga gccttgtcac tagccttgtc
     6241 attggttccc tgatgataag ggctggctgt catgatggca gggatttttc catcaaaacg
     6301 agggcgaata atgctaacct ttactaggtc tggtagccct ttttggtcag tatcgacacg
     6361 agactcaacg taaacgactt cacgaatgac atcctggcta gaaaaagtag ccaaactctt
     6421 gccgttaaag tagtggtagt cattatcctc cggaataaga ccatcactaa caagttggtc
     6481 gataagagta tttccttttt tggtgcgagt attgagtaac tgatagagat tttcaatcaa
     6541 gtcaccatat ataatgggaa atccagtttc tttacgaaaa acgtcactat cttcgaagtc
     6601 aaccaaataa gcaaagccta aaagttgaaa agcaacagta taaaaaatat ctgctgtcag
     6661 ttcattttcc gattgaaaaa atgtcagcag gtctgttttt ttatcagctg ctaggatgga
     6721 aagtgggtag ttggtgtctt gataagtgaa aaagaaacga cgtaaaaagg tttcaagtga
     6781 gtctttgtga ttggctgtat tttgtaaatt aaagccacat ttttttagtt cagataagac
     6841 attttctttt ggaaaattga tataactata ttgattaaaa cgcatagaac ctccatatag
     6901 aatgacagtt aaggttatta tatcaaaaaa aaagcagaaa gggaattgtt aacttcaaaa
     6961 ggaaataatc caataaaaat gaataaagta ctaaattcaa tatagagaac agagtaacaa
     7021 taagaataat agatagggta ta
//
LOCUS       DAVKDZ010000090         7030 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000090 DAVKDZ010000000
VERSION     DAVKDZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7030
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.091"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            514..1635
                     /gene="mnmA"
                     /locus_tag="VWZ30_001865"
     CDS             514..1635
                     /gene="mnmA"
                     /locus_tag="VWZ30_001865"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU9335543.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVIAVADQISIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLNQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLATAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVAGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFMREMPEEFTLECTAKFRYRQPDSKVTVHVKGEKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1776..2231
                     /locus_tag="VWZ30_001866"
     CDS             1776..2231
                     /locus_tag="VWZ30_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9335544.1"
                     /translation="MTQQDFRTKVENTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEGAVVREVREELGVKAQAGQLAFVVENHFEQDGVYYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTTLPDWDGQLKHIHREE"
     gene            2241..4154
                     /gene="mnmG"
                     /locus_tag="VWZ30_001867"
     CDS             2241..4154
                     /gene="mnmG"
                     /locus_tag="VWZ30_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU9335545.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEAMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPVNIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(4361..4453)
                     /locus_tag="VWZ30_001868"
                     /pseudo
     CDS             complement(4361..4453)
                     /locus_tag="VWZ30_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4507..6186)
                     /locus_tag="VWZ30_001869"
     CDS             complement(4507..6186)
                     /locus_tag="VWZ30_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9335546.1"
                     /translation="MAYTLKPKEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKTP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(6188..6421)
                     /locus_tag="VWZ30_001870"
     CDS             complement(6188..6421)
                     /locus_tag="VWZ30_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEU9335547.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRIAARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
BASE COUNT         1972 a         1430 c         1657 g         1971 t
ORIGIN      
        1 gaaaaataga ttagagaaaa tgggttgttt tatttattat agttatttga atgaaaataa
       61 gaagaaggta tactcacgtc attcacatga tctgtatatt gactataagt tttaaaaaac
      121 aatttttaag ctctttcttg tcttctctaa ccaagcgtgt tataatgaat actgctcaag
      181 cgaccttcaa tcgttaagca cacacgacct tcaatcgtga actagtcatt tcgtttatct
      241 tttattacgt cgttaactcg aaataagatg caagctgagc ttgtctgatt tataacctca
      301 atcagtctcc cagactgatt gagcgaactc aagttatgcc tgcgactcgt tgcctatcat
      361 attttcattt taagatgata ggctaaaatg gtcttccaga ccattttact actaaaagca
      421 aacgaaatga ctatactcaa ataaattcta ttgaaagtca tagtaaatag aattttgagg
      481 ggtataaata aacgaataga tgggagactt accatgagtg ataactctaa aacacgtgtt
      541 gttgtgggga tgagtggtgg tgttgattcg tcggtgacgg ctcttttgct caaggagcag
      601 ggctacgatg tgatcggtat cttcatgaag aactgggatg acacagatga aaacggcgtt
      661 tgcacggcga ccgaagatta caaggatgtg attgcggtgg cagaccagat tagcattccc
      721 tactactctg tcaattttga aaaagagtac tgggaccgcg tttttgagta tttcctagcg
      781 gaataccgtg cagggcgcac gccaaatccg gacgttatgt gcaacaagga aatcaagttc
      841 aaggcctttt tggactatgc catgaccttg ggggcagact atgtagcgac tgggcattat
      901 gctcgagtgg cgcgtgatga ggacggcact gttcacatgc ttcgtggcgt ggacaatggc
      961 aaggaccaga cctatttcct caaccaactt tcgcaagaac aactccaaaa aaccatgttc
     1021 cctttgggac atttggaaaa gcctgaagta cgcagactag cagaagaagc aggcctggcg
     1081 actgctaaga agaaagattc gacagggatt tgttttatcg gagaaaagaa ctttaaaaac
     1141 ttcctcagca actacctgcc agctcagcct ggtcgcatga tgactgtggc tggtcgcgat
     1201 atgggcgagc atgcaggtct tatgtactat acaatcggtc agcgtggcgg actcggtatc
     1261 ggtgggcagc acggcggtga caatgcccct tggttcgttg tcggaaaaga tctaagcaag
     1321 aatattctct atgtaggaca aggattctac catgattcgc tcatgtcaac tagcctagaa
     1381 gccagtcaag tccattttat gcgtgaaatg ccagaagagt ttacgctaga atgtacggct
     1441 aaattccgtt accgtcagcc tgattctaag gtgacagtac atgtcaaagg agaaaaggca
     1501 gaggtcatct ttgcggaacc acaacgcgcg attacaccag gacaggcagt tgtcttttac
     1561 gatggcgaag agtgtctagg tggcggtttg attgacaatg cttaccgcga tggacaagtt
     1621 tgtcagtaca tttagattga caaattttct caatttgcta caataataac agcaatagaa
     1681 atgatggtca aagctcatgg atgttgcagg cttttttgtc ctgcacttct ttgtagtttt
     1741 gactgttttt gtgtcgttta agggaaagga taaaaatgac tcaacaagac tttcggacaa
     1801 aagtagaaaa tacggttttt ggtgttcggg cgacagcctt gattgtccaa aatcacaagc
     1861 ttctagttac caaagacaag ggcaagtatt acactattgg cggtgcgatt caagtcaatg
     1921 aaagcacgga aggcgcggta gtccgtgaag ttagggaaga actgggtgtc aaagctcaag
     1981 ctggccagct agcttttgta gttgaaaatc attttgaaca agacggtgtc tattatcaca
     2041 atatcgagtt tcattatctg gtagacttac ttgaagatgc cccattgacc atgcaggaag
     2101 atgagaaaag gcagccctgt gagtggattg acttggataa gcttgaggat atccagctag
     2161 ttccagcctt tttaaaaaca accctacccg attgggacgg ccaactaaaa cacattcatc
     2221 gtgaggaata ggagagaaac atgacttatc attttactga agaatacgat attattgtaa
     2281 ttggtgcggg acacgctggg gttgaggctt ccttagctgc tagccgtatg ggctgcaagg
     2341 tcttgcttgc gaccatcaat attgaaatgc tggctttcat gccttgtaat ccctctatcg
     2401 gtggttctgc taaggggatt gtcgtacgtg aagtcgatgc cctcggtggc gagatggcca
     2461 agaccattga caagacttac atccagatga agatgctcaa cacagggaag ggaccagccg
     2521 ttcgtgccct tcgtgcgcag gctgataagg aactttactc taaggaaatg cgcaagacag
     2581 ttgaaaatca agaaaatctg acccttcgtc aaaccatgat tgatgagatt ttggtggaag
     2641 atggcaaggt tgtcggtgtg cgtacagcca cccatcaaga atatgctgct aaggctgtta
     2701 ttgtgacgac agggactgct ctccgtgggg aaattatcat cggagacctc aagtactcat
     2761 caggtcctaa ccacagcttg gcttctatta acctagctga caatctcaag gaactgggtc
     2821 tcgaaatcgg tcgtttcaag acaggaaccc ctccacgtgt caaggcctct tctatcaact
     2881 acgacgtgac agaaatccaa ccaggagatg aagcgcctaa tcacttctca tacacttcac
     2941 gtgatgagga ttatgtcaag gaccaagtgc catgctggtt gacctatacc aatggtacca
     3001 gtcatgagat tatccaaaac aacctccacc gtgcgcctat gtttacaggt gtggtcaagg
     3061 gagtgggacc tcgttactgt ccgtcgattg aagacaagat tgtgcgcttt gcggacaagg
     3121 aacgtcacca actcttcctt gagccagaag gacgtaatac agaggaagtc tatgtgcaag
     3181 gactttcaac cagtctgcct gaggatgtcc agcgtgactt ggttcattcc atcaaaggtt
     3241 tggaaaatgc agagatgatg cggacaggtt atgctattga gtatgatatg gtcttacctc
     3301 atcagttgcg tgcgactttg gaaaccaaga aaatctcagg tctcttcact gctggtcaga
     3361 caaatggaac gtcaggttac gaagaggcag caggccaagg gattatcgcg ggtatcaatg
     3421 cggctctgaa aatccaaggc aagcctgagt tgattttgaa gcgcagtgat ggttatatag
     3481 gggtgatgat tgatgacttg gtgaccaagg gaaccattga accctaccgt ctcttgacca
     3541 gtcgtgctga ataccgtctc attcttcgtc atgacaatgc tgatatgcgc ttgactgaga
     3601 ttggccgcga gattggcctt gtagacgatg aacgctgggc tcgttttgaa atcaagaaaa
     3661 atcaatttga taatgagatg aaacgcctag acagtatcaa actcaagcca gtcaaggaaa
     3721 ccaatgccaa ggttgaagcg atgggattca aaccgttgac agatgcagtg acagccaagg
     3781 aattccttcg ccgtccagaa gtttcttacc aagatgtggt agccttcatc ggaccagctg
     3841 cagaggattt ggatgataag attatcgaat tgattgaaac agaaatcaag tacgaaggct
     3901 atatttcaaa agccatggat caggtagcca agatgaaacg tatggaagag aaacgcattc
     3961 cagtcaatat cgactgggat gacattgact ctatcgcaac cgaagcccgt cagaagttca
     4021 aactcatcaa tccagaaacc atcggccaag ccagccgtat ttcgggagta aacccagcag
     4081 atatttctat tttgatggtg tatctggaag gtaaaaatcg tagtatttct aaaactcttc
     4141 aaaaatcaaa atgatacgtc gtcggcttct tacgaatgag ttcaaagctt ggctttgatt
     4201 catctccagc ctcccatagt tccccgaact atgggagcta actcaagtta tgctttcgct
     4261 tagccttcta gtctgatttc gatttttatt gatactcttc gaaaatctct tcaaaccgcg
     4321 tcaacgtcgc cttgccgtag gtatggctac tgacttcgtc agttctatct acaacctcaa
     4381 agcagtgctt tgagcaacct acggctagct tcctagtttg ctctttgatt ttcattgagt
     4441 atgagtttta atctcaaaag aaagcataga gaaaaacagc tccgaggacg gggctgtttt
     4501 cttgctttat tcttcatctg gtgtgaggat catcgggatg atgatcggtt cacgttcggt
     4561 attttcatag aggaaggggc gaatagcgtt gacaatggca ccattgacag attgcacgct
     4621 agcatcctta tttttcagtg cgatacgaat ggcattgaag aggatacgct ggctttggcg
     4681 aatcaagtcg ccagactctc tcatgtagac aaagcctcgg ctgaggatgt ctggaccaga
     4741 tagaatcatc tgcgatttga agtcaacagt tgcgactgcc agaacgacac cgtcttcaga
     4801 tagatcgcga cgatctttga ggacagctgc gccaatttca ccgatacgat ttccatcgac
     4861 atagatatct tgggcgttga aatgacctgc gatacgagct gagtcagcag taagggcaag
     4921 cacatcgcca ttgctcatga taaagatatt gtccttctca acaccagtat ccactgctag
     4981 tccagcgtgg actttttgca tgcggtattc accgtggaca ggcatgaagt attttggctt
     5041 aatcaagcag agcatgagtt tttgctcttg ctgaccaccg tgtccagatg tatggatatt
     5101 gttcacttta ccgtggataa cttcgacacc agcttcagaa atgatgttaa tcagcttgtt
     5161 gacgctagta gtgtttccag ggatgggact agaagagaag ataacggtat cacctggttg
     5221 taattgtact tgacggtggg ttccgttggc gatacgagag agggctgcca taggctcacc
     5281 ctgactacct gtacagagga taagaacttc tcctgcagga taatctttga tttcatttgg
     5341 ctcgataaag gttcccttag gagttttgat gtagccaaga tcgattccgt tgacaatggc
     5401 cttttccata gaacgaccaa agaccgcaat cttgcgtcca gtcttaacag cagcttctgt
     5461 tgcctgctgg agacggaaga tatttgaggc aaaggatgca aagatgatac gtccttcaat
     5521 accttggata atcttcataa tggactgacc aacgactttt tcagagttgg taaaggttgg
     5581 tacttccgca tttgtcgagt cagacaggag acagagcacg ccttcttcac caagcgcagc
     5641 catacgatgc aagtccgcag gttctccaac tggagtaaag tcgaacttaa agtcacccgt
     5701 acagacgatt ttcccttgag gagtatgaat gacaatcccc aaaggctctg gaatagagtg
     5761 agtcgttcta aagaaagttg ccttgagatt tttaaaggtc aactcggtgt tgtggttgat
     5821 ttcgtaaagt ttggcgttgc gcaagaggcc gtgttcttcg agtttcccac ggatcaaagc
     5881 caaggcaagc ggtccagcat aaatagggac atttgcttgc ttgagtagga acggaatccc
     5941 accaatgtgg tcctcgtgtc cgtgtgtgat taaaacagcc ttgacgcggt cgatattgtc
     6001 cacgatgtaa gagtagtcag gaatgacata gtcgatacca agcaagtcat cttctgggaa
     6061 tttaatccca gcatcgacga tgataatctc gtcttggtat tcaattccgt aagtgttttt
     6121 cccgatttct cctagaccac cgatggcaaa aacgccgact tctttaggtt taagagtgta
     6181 agccatatta gaactccgta atttcgaagg cgccagtttc tttttcgtaa tcgagcaatt
     6241 tgtcagacaa gagttcgata tattcgatat tgtactctgg gcgattttct tcgacaagtt
     6301 ggcgagcagc gatacggccc tcaagttctg agctggcatc gatgtctagg taaagcgcgc
     6361 gtgttgtttc gcgacgtggg ctacgttctt ttgtttcttg ataaaaaact ttgtaaatca
     6421 tatagttcct ttctatttac aggtcagctt attccaaact tttagcagag aattgcttga
     6481 tttcattcca ttttttgtct agattgacct tgattcgtta caattatttc aattatacca
     6541 caaaatgaaa aattagtaaa agacggaaac gagaaagtta ggggacgatt tcttcggtaa
     6601 gcgtttgcaa aaataaagaa aatgtgttat gttgataacg gaagtgaagt ttattacttg
     6661 tcataattcc atatagattt taagtcaagg aggttgtcct atgtataact tatttgcttt
     6721 tgctattgga gttcgtttgc tagcttttat cgtttttgtt gcttgcgttt atggaggaaa
     6781 tcttctattt gctaaactgg aacgatgata aaccaagttc ttgtggaaaa ttacctttgt
     6841 gactctctac tcgctttttc tcctcctcat gacctatgtc gtttggtttg tattttactt
     6901 tggattaaat gcttagatgc cctgcatatg tgaactgcac cccaaaagtt agacagaaaa
     6961 aatctaactt ttggggtgtt ttattatgaa attgagttat gaagataaag ttcagatcta
     7021 tgaactaaga
//
LOCUS       DAVKDZ010000091         7001 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000091 DAVKDZ010000000
VERSION     DAVKDZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7001
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.092"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            1..297
                     /locus_tag="VWZ30_001871"
                     /pseudo
     CDS             1..297
                     /locus_tag="VWZ30_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819222.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
     gene            351..587
                     /locus_tag="VWZ30_001872"
     CDS             351..587
                     /locus_tag="VWZ30_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335548.1"
                     /translation="MNDKIDNKQDNKTEEVNYKIVGDGREAENHINKSKEIVDVKQKL
                     PKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            1115..1429
                     /locus_tag="VWZ30_001873"
                     /pseudo
     CDS             1115..1429
                     /locus_tag="VWZ30_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            1609..2268
                     /locus_tag="VWZ30_001874"
     CDS             1609..2268
                     /locus_tag="VWZ30_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEU9335549.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRVTEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVRFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVSYHEKF
                     K"
     gene            2293..3360
                     /locus_tag="VWZ30_001875"
     CDS             2293..3360
                     /locus_tag="VWZ30_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9335550.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDAKEMPKDWT
                     ELASKYKGQYSIYGLTGGTGRAIYASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            3463..4473
                     /locus_tag="VWZ30_001876"
     CDS             3463..4473
                     /locus_tag="VWZ30_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9335551.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            4485..6176
                     /locus_tag="VWZ30_001877"
     CDS             4485..6176
                     /locus_tag="VWZ30_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9335552.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            6179..6889
                     /locus_tag="VWZ30_001878"
     CDS             6179..6889
                     /locus_tag="VWZ30_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU9335553.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2420 a          945 c         1334 g         2302 t
ORIGIN      
        1 gtgataattt agtagtctca gagaatagta tattcggtgg taaagaagga attgttattg
       61 aggattcaaa gggtaaaata accgttttaa ataaccaatt ttataattta tccggtaagt
      121 atatatcatt catcaaatct aatgcaaatg ggaaagaacc tgttatacgt gatagcgatg
      181 gtaatttcaa tattgtaacg gagaatgggc tttacaaaat tgtaacaaat aatttaagtg
      241 ataaaaacga aaaagaaaaa aacaaagagg aaaaacaatc taattcaaat aatgtaattg
      301 atagtaacca gaagaacgga gagtttaact caagtaaaga taatagacaa atgaatgaca
      361 agatcgacaa taaacaagat aataagacag aagaagtaaa ctataaaata gttggagatg
      421 gcagagaagc tgaaaatcat attaataaat ctaaagaaat agtagatgta aaacaaaaat
      481 taccaaagac tggttcgaac aagattatgg aactattctt aacagtgaca ggaattggtt
      541 tacttttgac actaaaaggg ttgaagtatt atggtaaaga taaataaaat ttgttcgatt
      601 acaaggaagt tccgtagaaa atgaggatat tgtaggttca caaaatcagt atttttggat
      661 tattggtgga gctacagatt tatataattc taaagaggag ataggttatt cagtctcaga
      721 agttgtacat attttatcag aaagtttgtc ggttaattgt aaagaatcaa aaactcttaa
      781 acaaatattt gaaactgctt tactcgaggt taaagatgaa atcggtttaa actcatatga
      841 attaacagag tatagtaaaa tgaaataata ataggacaaa tcaatcagga cagtcaaatc
      901 tatttttaac aatgttttag aagtaggggt gtactattct agtttcaatc tactatatta
      961 tcgcttacca acatttgctt ttatatttgc taaactttca gaaaaaagtt ggagtatatg
     1021 atgctaggtg attgcgtcat gttagtaaac gaaatggaaa taactgatca tagagtagat
     1081 aacttatttg aaaaagggaa aaatgagata aaagatccat gtagtagatt tagcaatagg
     1141 acatataaaa gctttagaaa aagtatctga aaaaacagat gtttatatct ataacctatg
     1201 ctcgggagaa ggcactagtg tattacaact tgtaaataca tttgaaagtg ttaataagat
     1261 ctctattcct tataaaatag ttccaagacg ttcaggagac gttgcaactt gttatgcaaa
     1321 tgcagacaaa gcatataagg aattaaattg gaggacaaca aaatcgattg aagacatgtg
     1381 tagagataca tggaattggc aatcaaaaaa tcccaatgac tataattgat gatactgtct
     1441 atatgagtgc tgtattttgg aaaaattact taacctttta atgaaatgca tatttaatga
     1501 aagataaatt aaaaaaaatg tttgacagcg cttgacaaag gaaacggttt cattgtataa
     1561 tgtaaacata gtgacaccga tatcaaatat aaaaaagaag gtctttatat gaaaaactta
     1621 acaaaaatta agttcaaaga gaatggggaa tttaatcatt tccctgggaa tacagttgta
     1681 gcaaatcttt atactaaaca agatttgatg gaagttgttg atattattca atcacgttat
     1741 agagaattac catttatcga taagtttact ttaactccaa ggaattctat tcacatgact
     1801 gtaattgaat tgttgtgcca tgaaaatcgt gaaacggagt tttggagcag taatcttcct
     1861 ctagatacac ctttacagga aatacatgat tactttgcta aacaacttga aatttttcca
     1921 ttgttggatg aagaaattca tatgcgtgta actgaaatgg gaaaacaaaa catactagtt
     1981 gaacctgcag atgaagcttc tgcaaagaga ttagaagaaa ttcgtactta tgtctcagaa
     2041 aaagcaggtg ttcgtttccc taatcatgat agatatcaat tccatatttc aattgggtat
     2101 cttcggattc ctctaaccga agaggaggaa gaagagttta ctaaagtcag agcagaatta
     2161 actgaaattt tattagagaa gattccaact attactgtaa accgtattga ctatactgta
     2221 tttgaagata tgagacaatt tgtttcatat cacgaaaaat ttaaataaaa taataaaaaa
     2281 ggagaactta tcatgaaaaa aatgaaagtt tggtctactg tacttgcaac gggagttgct
     2341 cttactacac ttgctgcttg ctctggaggt tcaaattcta cgactgcttc ttcatctgaa
     2401 gaaaaagctg ataaaagtca agaattagtt atctattcga actcagtctc aaatggtcgt
     2461 ggtgattggt taactgctaa agcaaaagaa gctggtttta atataaaaat ggttgatatc
     2521 gctggcgctc aattagcaga ccgtgttatt gctgagaaga ataatgcagt tgcagatatg
     2581 gtatttggaa ttggtgctgt tgactcaaat aaaattagag atcaaaaatt actagtacag
     2641 tacaagccta aatggttaga taaaattgat caatctttat cagataaaga taattattat
     2701 aacccagtta ttgttcaacc cttggtattg attggagctc ctgatgcaaa agaaatgcca
     2761 aaagactgga cagagttagc tagtaaatat aagggacaat attccattta tggcttaact
     2821 ggtgggactg gacgcgccat ttacgcaagt attctagttc gttatcttga tgaaaaaggt
     2881 gatttaggag tatcggaaaa gggctgggaa gttgcaaaag aatactttgc aaatgcctat
     2941 actcttcaaa aaggtgaaag ctcagttgtt aaagcgttag ataaagaaag cccaattcaa
     3001 tacggtatga tctggggttc tggagcgtta attggacaaa aagaacaaaa tgttgaattc
     3061 aaagttatga ctcctgagat tggtgtacca tttgtaactg aacaaactat ggttttaagc
     3121 actagtaaaa aacaagcgtt agctaaagaa tttattgatt ggtttggtca atcagaaatt
     3181 caagtagaat atagtaaaaa atttggctct atccctgcca ataaagatgc cctaaaagag
     3241 ttacctgaag atacgaaaaa atttgttgat caagtgaaac cacaaaatat tgactgggaa
     3301 gctgttggaa agcatttgga tgaatgggta gaaaaagctg aattagaata cgtaaaataa
     3361 gaaataagag ttatttttta tgtccaagtc tctctattat ttctaaattt aaggaagaaa
     3421 taaagagagg cttttggttt agtaatagag ggagttagtg agatgattaa atttgataat
     3481 attcaaatta aatatggtga ttttgttgca attgataatc tgaatttaga tatacatgaa
     3541 ggggaattct ttacatttct tgggccttca ggatgtggta aatcaactac tttgagagca
     3601 ttggtaggtt ttttagatcc atcatcagga agtattgaag ttaatggaac agatgtcact
     3661 catttggaac ctgaaaagcg tggaattggt attgtatttc aatcttatgc gctatttcca
     3721 actatgactg tttttgataa tattgcattt ggtttaaaag ttaagaaggt agctccagat
     3781 gttattaaag ctaaagtatc agcagtggca gcaaaaatta agatctctga tcaacagtta
     3841 cagcgtaatg tatcagaatt atctgggggt caacaacaaa gggtagcatt ggctcgtgct
     3901 ctggttcttg aacctaaaat tctttgtcta gatgaaccat tgtcaaacct tgacgcaaaa
     3961 ttacgtgtag atttgagaaa agagttgaaa agacttcaaa aagagttagg tattactact
     4021 ttatatgtta ctcatgatca agaggaagcc ttgactttat ctgatagaat tgcagtcttt
     4081 aacaatggat acatcgaaca ggtcggtaca ccagtagaga tttatcataa ttctcaaact
     4141 gaatttgtat gtgattttat tggagatatt aatgttttga ccgatgaaac agtccacgaa
     4201 gtattattga aaaatacaag cgttttctta gaggataaaa aaggatacat tcgattagag
     4261 aaagttcgat tcaatcgtga aactgaacaa gattttattc taaaagggac aattattgat
     4321 gttgagtttt ctggagttac aattcactat acaataaaag tttctgaaag tcagattctt
     4381 aatgtaacaa gtattgatag tcaggctgct attagatctg tcggagaaag tgtggaatta
     4441 tttatcacac catcagacgt tctgcaattt taaggaggtg cagtatgcgt cataaattaa
     4501 atttaaaaga ttggcttatt cgtttagggt taatctggtt cttagtaaca tttattattt
     4561 atccaaactt tgatctagta gtgaatgtat ttgtaaaagg aggagaattt tcccttgatg
     4621 ctgtacatcg tgttctaaaa tctcagaggg cacttcagag tattatgaac agttttaagt
     4681 tagcattttc actcattatt acagttaatg tcgtaggtat tctttgtgtt ctatttacag
     4741 agtactttga tattaaaggt gctaaaattt taaaattagg ttatatgacc tctttaattt
     4801 atggaggagt ggttttagcg actggatata aatttgtcta tggtccttat ggattgatta
     4861 caaaattttt acaaaatgtt atcccttctt tagactctaa ctggtttatt gggtatggtg
     4921 cagtcttatt cattatgaca ttttcaggaa ctgctaatca tacattgttt ttaacaaata
     4981 caattcgaag cgttgactat cacactattg aggctgctcg aaatatggga gcaaaaccat
     5041 ttactgtttt ccgaaaagta gtgttaccaa ccttaattcc aactctattt gcacttacta
     5101 ttatggtttt tcttagtggt ttatctgcag tagcagcacc catgattgtt ggtggtaaag
     5161 aatttcaaac tataaatcca atgattatta catttgcagg gatggggaat tctcgtgatt
     5221 tagctgcctt acttgcaatt attttaggta ttgcaactac aattttgctt actatcatga
     5281 ataagataga aaaaggtgga aattatattt ctatctctaa gactaaagcg cctcttaaaa
     5341 aacaaaaaat tgcgtctaag ccttggaata tcattgctca cattgtagca tatggattgt
     5401 tcacagtttt catgcttcca ctaattttta tagtattata ctcatttaca gatccagttg
     5461 caattcaaac aggtaactta acattatcaa actttacttt agaaaattat cgcttattct
     5521 ttagtaatag tgcggcattc tctccattct tggtcagctt tatttattct attattgctg
     5581 cgacaacagc aacaattctc gcagttgtat ttgctcgtgt tgtcagaaaa cataaatctc
     5641 gttttgattt cttatttgaa tatggtgctc tacttccttg gttactacca agtacacttt
     5701 tagcagtaag tttattattt acttttaatc agccacaatt tcttgtcttg aatcagattt
     5761 tggtaggtag tttggtaatt ctacttattg catatatagt tgtaaaaatc ccattttctt
     5821 atagaatggt acgtgctatt ttatttagtg ttgatgatga gatggaagat gcagcaagaa
     5881 gtatgggtgc ttcacctttt tatactatga tgaaggttat cattccattt attttaccgg
     5941 ttgttctctc tgttattgct ttaaacttta actctttatt aactgacttc gacttatctg
     6001 tattccttta ccatccccta gctcaaccat taggtattac gattcgatct gcaggtgatg
     6061 aaacagcaac atctaatgca caagctctgg tatttgttta tacaattgtt ctgatgatta
     6121 tttctggaac ggtattatac ttcacacaaa gaccggggcg taaagtaagg aaataatcat
     6181 gacagccact agtcttgggt tatcaaatat tgaaatagtt gtcaggattg ttttatcagt
     6241 agtcattggt agtataattg gtttagagag agggagcaaa tcccagcctg caggcatccg
     6301 aacttatagt attgtttgtc tagctgcatg tttgattatg atgacgaatg aatacgtatc
     6361 ttataaattt gggacaggag atcctacacg attaggagct caagttatat caggtgtggg
     6421 ttttctaggc gctggaacga ttcttattac agataaaaag aaaattacag gtctgacaac
     6481 tgcagcaggc atttgggctt cggcaggaat tggattagct attggagtag gtttttatga
     6541 gggagctctt ttagtagcca tttctgtttg gggtgtgata tccatgttcc aaccactaaa
     6601 aaaatatctg caaaatcgtt ctaaaatgat tgaattgtat atagtggtta aatctacaga
     6661 agcctataat cgtgtattgg tttattgtgc agaaaatggt atcagaatga ccgattcaag
     6721 aacagcgttt ggagatgtta attctgacag aattgaatac tttgatgttc cggacaaaaa
     6781 aatagcgtca tttattactc tggaattatc aggtagattt gaacatcttc gactgatgga
     6841 agaaattgct aatattgtag gtgttattta tgtcgaagaa attagctgaa atttagtaaa
     6901 caaagggaca gatgtttttg tccttttgct ttttagatgt tttaatcatc taatttttga
     6961 gtagattttt gctcagaggt ttttatgaaa gattataaaa t
//
LOCUS       DAVKDZ010000092         6971 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000092 DAVKDZ010000000
VERSION     DAVKDZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6971
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.093"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(114..389)
                     /locus_tag="VWZ30_001879"
     CDS             complement(114..389)
                     /locus_tag="VWZ30_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU9335554.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(496..1335)
                     /locus_tag="VWZ30_001880"
     CDS             complement(496..1335)
                     /locus_tag="VWZ30_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9335555.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDIEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRVVMQMKNHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1476..2324
                     /locus_tag="VWZ30_001881"
     CDS             1476..2324
                     /locus_tag="VWZ30_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9335556.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2417..3334)
                     /locus_tag="VWZ30_001882"
     CDS             complement(2417..3334)
                     /locus_tag="VWZ30_001882"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU9335557.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3772..3977
                     /locus_tag="VWZ30_001883"
                     /pseudo
     CDS             3772..3977
                     /locus_tag="VWZ30_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4288..4581)
                     /gene="rpmA"
                     /locus_tag="VWZ30_001884"
     CDS             complement(4288..4581)
                     /gene="rpmA"
                     /locus_tag="VWZ30_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU9335558.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4598..4942)
                     /locus_tag="VWZ30_001885"
     CDS             complement(4598..4942)
                     /locus_tag="VWZ30_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU9335559.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(4958..5272)
                     /gene="rplU"
                     /locus_tag="VWZ30_001886"
     CDS             complement(4958..5272)
                     /gene="rplU"
                     /locus_tag="VWZ30_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU9335560.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5308..5395)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5454..6017)
                     /locus_tag="VWZ30_001887"
     CDS             complement(5454..6017)
                     /locus_tag="VWZ30_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEU9335561.1"
                     /translation="MTFCAGYVNAYTFIVRGNTLVAGQAGNVVFLSVDLIQNNVSDVR
                     DKLLTLIAFIMGVFLLTIYKEKLRIVKKSILSLIPLAILSIIIAFVPQTVDNIYLVPP
                     LAFCMGLVTTAFGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILG
                     VPIMMSVFYLSMLFASWQKKVKEKASF"
     gene            6488..6787
                     /locus_tag="VWZ30_001888"
     CDS             6488..6787
                     /locus_tag="VWZ30_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU9335562.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         2210 a         1404 c         1156 g         2201 t
ORIGIN      
        1 acaatatttt cggaacattc aactttttaa ctctatttat tactagattt cataattaaa
       61 aagcctactg accaagctag aaagcttgat acaataggct ttttaaagac tgattattta
      121 acagcgtctt taagagcttt accagctttg aatgctggta ctttagaagc tgcaattgtc
      181 atttctttac cagtttgtgg attgcgacct ttacgttctg cacgctcacg aacttcaaag
      241 ttaccaaaac cgatcaattg aactttttca ccagctgcaa gatagtcagc tactgctgca
      301 aatacagctt caactgctgc tgctgagtct ttcttagtca actctgtagc ttctgctact
      361 ttagcgatca aatcttgttt gtttgccatg ttaatgattc ctccaaataa tttctaatta
      421 acaaatataa tcatatccta aaatcccttt taggtcaagt taaaaacgct atttcttccc
      481 attttattta tttttttagg agtggtaacg tatcaaaata gcccaagcgt tctcacccgt
      541 gtgagtttga ataatggaac ccgtttccaa aacagaaatt ggcttttcaa cataagcttg
      601 taagctttct ttcatctctt ttgcccaatc atcactacca gaatatgaaa ttccaatctc
      661 tgctacagca cgttcagaaa gcgatgttat caactcatct aaccattttt taaatgtttt
      721 agttccacga cctttaacca ttggctgcaa ttcatggttt ttcatttgca tgacaacacg
      781 gatattgaga agagagctca acaagccagt tacacggcca attcgtccac ctttgacaag
      841 attttccaaa gttgaaacac caatataaag ctctgtatgg tttttaacct cttctacatg
      901 agataaaatt gcctctatat ctttaccttc ttgagctaac ttcgcagcct caacaacttg
      961 gaatttcagg gcttggtcag tgaaggaact atcaacaaca gtcacatctg cagtagatag
     1021 gctagcacct tggcgtgctg cttctaccgt acccgaaaga gcatgggaca tatgaatagc
     1081 aagaatttgg ccaccatctt tgcataggtc ttcaaaaatc tcagcaaaga cacctacagg
     1141 tggctgactt gttttcggaa gattcttact ttcttgcatc aactgaagaa atttaccttc
     1201 ttctttcaaa tccgcatcag aataaacaac attatcaatc attacagata atggaacaat
     1261 tgtaatatct aattgcttta ctagttcagg ttcaatagta acagatgaat cggttacaat
     1321 cttaattttt gtcatagtat caatctttct attttaggat tcagattggt ttccttactt
     1381 ctaattatat caaaaaaaaa ttaaaaatcc taatggagtc aatcaaattt tccgtaaaat
     1441 ttgatataat caacttataa gaaaagaggt gtcctatgat taaaaaaatt taccccattt
     1501 ttaccatttt actaggtgct gctatttatg cttttggact gacttatttt gtagttcccc
     1561 atcatctctt tgaaggaggg gcgacaggca ttaccctcat caccttttat ctttttaaaa
     1621 tccctgtttc cctcatgaac ctgctgatta atattcccct tttcatccta gcttggaaga
     1681 tctttggagc caaatccctc tattctagtt tactaggaac cttagctttg tccgcctggt
     1741 tagctttttt tgagcatatt ccccttcata ttgatcttca aggtgattta ctaatcacag
     1801 cccttatagc gggaatccta ttgggaattg gccttggaat tatttttaat gctggaggta
     1861 caactggcgg aactgatatt ctagctcgta ttctcaacaa atacactcat atatccatag
     1921 gaaaactgct ctttatctta gatttttgta ttctcatgtt gattctccta atcttcaagg
     1981 atttgagatt ggtttcctac acgcttttgt ttgattttat tgtttctcgt gttattgatt
     2041 tgattggtga aggaggatat gccggcaaag gctttatgat tatcacaaaa cgtcctgacc
     2101 aacttgctaa ggcgattaat gatgacctcg gaagaggtgt tacttttatt tctggtcaag
     2161 gctactatag taaagaaaat ttgaaaatca tctactgtat tgtcggaaga aatgaaattg
     2221 tgaaaacgaa ggaaatgatt catcgaatcg atcctcaagc ctttataact attacagaag
     2281 cccatgaaat cctaggagaa ggcttcacct ttgaaaaaga ataaaaagag gtaatgtcgt
     2341 gacctcaaaa gttagactaa atcatctatc ttttgggtta cagacaacct cttttttgtt
     2401 ttatttattc aagctcttaa gaccaattcc gagttacttc ttcatcagcc tttaactgat
     2461 ccactaattg gtcaactgag tcaaatttgg tcatatcacg aatgcgatca agccaataaa
     2521 ccatgacggt ttccccataa atatcttgat taaaatcaaa aatattgact tcaaaacgtg
     2581 cttcttctcc atcaaaggtc acatttttcc cgacactagc catagcacga tacttctgtc
     2641 tttgaatctc aacatcaacg acataaacgc catctgctgg catataagta cggtctaaaa
     2701 gcactaaatt cgctgtcgga taaccaattg tacgaccacg agcattacca tgaactacca
     2761 tacctcttga tggaagcggt gcccccaaaa gttttcctgc ttctttcaca tttccatcta
     2821 aaatagcttg acggatacga gttgaactaa tctttccttt ctcatcttct acaggtggaa
     2881 caatgataac ttctccatca aagtaatcct ttaaatcttc tgctgttttt ttgtcagaac
     2941 caaatgtata atcaaaacct gcaacaataa ttttggcatt catagccttg atataagttg
     3001 caaagaattc ttgtgcagtg agactagcga attgactact aaaatcaagg agatataatt
     3061 cttctacacc ttcgcgcttt aattttcttt cacgttcagc agggttcaaa atatgcaaaa
     3121 acaaatctgg atgataaggc tctaaagcga tctttggaga ttcattaaag gtcataacga
     3181 cgataggcaa taaatccttt ctcgcagcct tgttggcaac acgaaataat tcttgatgcc
     3241 ccttatgtat gccatcaaaa tagccgagaa caacgactga atcagatggt gtgccaatat
     3301 ctttttggtt ttttatagga atagtaataa tcataaaata attatatcat agcgatagct
     3361 atttctggaa cagaaaatct gaaatgttgt ttttttcaca tgaagtgtac ctgttttcaa
     3421 aaggcacttt attctatcgt tgcttaacta tgaactttgc aatattcttc tcaaaaactt
     3481 gtaggacatc ttcaaaattt tgcaaggagt gattagactt gttcggtaac cataaagtgt
     3541 catactatgc ttatgtatga aaaagcaatg caactaactc ctgagaactt taaattacta
     3601 attggtgccg aaaaggtaga atttagaatc gaggtacacc tatggctgta aaatttacaa
     3661 aatgagacaa cttgggcaag atgtttgaag aatttcctaa actccctgat ttgaagcaag
     3721 tcactttccc tgatgacaaa gaaaaaagcc aaagcaataa agaaaaacta gatgactgct
     3781 ttccaacaac tcccatctag tgtgcttcag actgggctat ttttctctcc atctgttagc
     3841 ttggattctc agaccgtttc agctaaagaa tatcttttcc cttatcagaa ggaacggctc
     3901 aagccattca gacaagtgaa gggacgacaa gccaatattt gaaaccagat agcagttctt
     3961 atagtcaatt gaaataaaat ctgaagaaat cgagtaggaa actcatatca atgtttaaca
     4021 gtgttctatt ccagattcat actcaatgaa aattaaagtg caaactagga agttagccgc
     4081 aggctgtact tgagtacggc aaggtgaaac tgacgtggtt tgaatttgat tttcgaagag
     4141 tatcaatcca ctatacatat taaaaagtat gggtatcaaa ataaaaaaac aggctctccg
     4201 aaaactcgga aagccttatt ttaagctgtt tctagcttcc tcacctttac atttcaaggt
     4261 tcgggataaa aggttcaatg aaccttttta ttttgcgatt gggtaaactg aaacttgttt
     4321 tttatcgcgt cctttacgtt caaagcgtac tacgccttca actttagcga acaaagtatc
     4381 gtctccacca cgaccaacgt ttacacctgg atagatgtgt gtaccacgtt gacggtaaag
     4441 gattgatcca cctgttacag tttgtccgtc agctgcttta gctccaagac gttttgcttg
     4501 tgaatcacgt ccatttgatg tagaacctcc accttttttg tgggcgaaaa gttgcaagtt
     4561 gttaagagtc atttttaaca taatgttttc ctccgtgtta gttttctgtg ataactctgg
     4621 tttggacgaa ctcagaagag ttctccgata agtttgccat acctaagaaa aatgattcaa
     4681 agaataactg ggtcatttct ctctggtgtg aaggaagatc ttttggtatt tcaaccatca
     4741 gatagccacc ttcatcttcg tttaattcta ggattggttc atagcctgca aatttctcaa
     4801 tagaattgat aaagttaatg gcaagcgtag aaaccgatgc acacacgaca tctaagccgt
     4861 attcgccact ctcggcgtgt ccagtaattt ccgcactcct cagctcgcca tcttcggctc
     4921 tctcaaagac tgcctgtatc atgtgttctc cttaaaatta agcgttgatt gcgttgatga
     4981 caacttttgt atatggttga cggtgacctt gtttacggtg gctacctttt ttaggtttgt
     5041 acttgtaagt aaccactttc ttttgttttc cttgtttttc aacagttcca actacagtag
     5101 ctccagcaac aagtggagtt ccgacaacag tgttttcacc accaacaaga acaacttcgt
     5161 taaaagtaac ttcttgacca gcttcaacgt tcaatttttc aacgtaaact gcttgaccaa
     5221 cttcaacttt aacttgtttt ccgccagttt tgataattgc gtatgtgctc attatgcacc
     5281 tcctatgatt tttatgggtt tccccgattt tttgtgaaga ctcgcctagc atcgtgggac
     5341 gaaccactta aacttgaata atcaagcaac gatgttcgtg cggttgcaca ggatcgtgca
     5401 tagtcaactc ttcaagtata gcatatctcc tattttctta caagtaataa cacctaaaat
     5461 gaagcttttt cttttacttt tttctgccaa gaggcaaaaa gcatgctgag gtaaaaaacg
     5521 ctcatcataa taggaacacc aagaatggtc ttttcatgat agaaaatcgt caaataggct
     5581 gaaaagacaa cgccaaggac aaaactacta agcaggctaa caaatatgaa tccttcacgc
     5641 aaaaaaggag tgtgcttggt tcggaaataa tctccaaaag cagttgtcac cagtcccata
     5701 cagaaggcca agggcggcac tagatagata ttatccacag tttgcggcac aaaagcaata
     5761 atgattgata agattgccaa gggaatcaag gacagaatag attttttcac aattctcaat
     5821 ttttccttat aaatcgttaa taaaaagact cccattataa acgctattaa ggttaaaagc
     5881 ttatccctaa catccgaaac attattttgt atgagatcta ccgaaagaaa gacaacattt
     5941 ccagcttgtc cagctacaag ggtattccct cgaacaataa aagtgtaagc attcacatat
     6001 ccagcacaaa acgtcaaaaa agtgctaacc ttttagactg acgtgatatt tttcttatag
     6061 gtaataacct cattttccct cccattgtat tttctcttag aaatattgta ccattttctt
     6121 tctaaaaaat cgcaggctac catttagatt ttactattag cataaaaata ataatagaca
     6181 actatttatc caaaaataga tagaggtaac atgtttgcaa acaaagcata cgaaccttta
     6241 gtaaaatcat ttccatgaaa ctagaataga gcccctctta gcaaaaatca ttattttaat
     6301 ttatttctaa tcactccttg acataaataa ctctcaccaa taaaagacta tgtcttaaaa
     6361 aaatggtata ataaaatcaa tacttgggct tgatgactat gctactaata acaattagga
     6421 gagaaaatca ggcacttgtt aacaacaagg attatcccct tgagatgaaa ggaactttag
     6481 aaatcttatg atgaacatgc aaaacatgat gcgtcaagca caaaaacttc aaaaacaaat
     6541 ggaacaaagc caagctgaac ttgctgctat gcaatttgtt ggcaaatctg ctcaagatct
     6601 tgtccaagcg accttaactg gcgataagaa agttgtcagc attgatttca atccagctgt
     6661 cgttgaccca gaagacctcg aaaccctttc tgatatgacc gttcaagcca tcaactctgc
     6721 tcttgaacaa atcgatgaaa ctactaagaa aaaactgggt gctttcgctg ggaaattgcc
     6781 attctaaaaa caaggagcta gaacaatact tgtcgataac aaagactaag aaaggtgcaa
     6841 aaattatagg ctctataata tttgtagtgg gtaaattccc tatggatatt atggagccta
     6901 ttttgtgtag aaaaaaagtc ccatatgacc tataatgaaa agcgacaaaa caactcatta
     6961 gaaagaatca t
//
LOCUS       DAVKDZ010000093         6879 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000093 DAVKDZ010000000
VERSION     DAVKDZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.094"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..441
                     /locus_tag="VWZ30_001889"
     CDS             <1..441
                     /locus_tag="VWZ30_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9335563.1"
                     /translation="ETGFDTYFYREYGRSLKGQLIRGKVSGRRYQRISLVAGLTNGEL
                     IAPMTYEETMTSDFFEAWFQNFLLPTLTTPSVIIMDNVRFHRMGKLELLCEEFGHKLL
                     PLPPYSPEYNPIEKTWAHIKKNLKKVLPSCNTFYEALFSCSCFN"
     gene            502..1467
                     /locus_tag="VWZ30_001890"
     CDS             502..1467
                     /locus_tag="VWZ30_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9335564.1"
                     /translation="MNMSNITIEDGQEMDLSQTMPSYAQVVSLYDDTSISVSNNETDK
                     VLAGSLYTDTNEQGLTIPSSLLKNWNEQTGRNLTANDLIGKSVSASIVESAAEASKIA
                     QFQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVT
                     EELQKNKKYTVLSQQKVLDIVIIFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQR
                     SKEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSI
                     TKVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1644..2651
                     /gene="gap"
                     /locus_tag="VWZ30_001891"
     CDS             1644..2651
                     /gene="gap"
                     /locus_tag="VWZ30_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU9335565.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2719..3555)
                     /locus_tag="VWZ30_001892"
     CDS             complement(2719..3555)
                     /locus_tag="VWZ30_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9335566.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQYLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVSQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3681..5876
                     /locus_tag="VWZ30_001893"
     CDS             3681..5876
                     /locus_tag="VWZ30_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9335567.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKIAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIANHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5929..6081
                     /gene="rpmG"
                     /locus_tag="VWZ30_001894"
     CDS             5929..6081
                     /gene="rpmG"
                     /locus_tag="VWZ30_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9335568.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            6091..6267
                     /gene="secE"
                     /locus_tag="VWZ30_001895"
     CDS             6091..6267
                     /gene="secE"
                     /locus_tag="VWZ30_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU9335569.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6322..6858
                     /gene="nusG"
                     /locus_tag="VWZ30_001896"
     CDS             6322..6858
                     /gene="nusG"
                     /locus_tag="VWZ30_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU9335570.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2144 a         1195 c         1485 g         2055 t
ORIGIN      
        1 gaaacaggat tcgatactta tttttatcga gaatatggtc gctcattaaa aggtcagtta
       61 ataagaggca aagtatctgg aagaagatat cagaggattt ctttggttgc aggtctaaca
      121 aatggtgaat taatcgctcc aatgacttac gaagagacga tgacgagcga cttttttgaa
      181 gcttggtttc agaattttct cttaccaaca ttaaccacac catcggttat tattatggat
      241 aatgtaagat tccatagaat ggggaagcta gaacttttat gcgaagagtt tgggcataaa
      301 cttttacctc ttcctcccta ctcacctgag tacaatccta ttgagaaaac atgggctcat
      361 atcaaaaaga acctcaaaaa ggtattacca agttgcaata ccttttacga ggctcttttc
      421 tcttgttctt gtttcaattg actatagttc acggatacag ttgggaaaga agctattaat
      481 tatttggaga gtccttttgg gatgaacatg tcaaatatta ctatagaaga tgggcaagaa
      541 atggatctga gtcaaacgat gccttcttat gctcaagtag tgagtcttta tgatgataca
      601 agtatctctg ttagtaataa tgagacggac aaagtgttgg ctggatccct ctatactgat
      661 acaaatgaac agggattaac gattccaagc agtttactaa aaaattggaa tgaacagaca
      721 ggaagaaatt tgacagctaa tgatcttatt ggtaaatcag tctcagccag cattgtagaa
      781 agtgctgccg aagctagtaa gattgctcaa tttcaaacga agattgtacg tgtaatcaat
      841 gatgaagatg acatggagga cagcaacagt ttcatgctgt ctcatcaaat ggaaacgatt
      901 ttgaaagagg ctggatttac gaaagctgta tcttatttta tcttggaact caaagatcca
      961 tcacagacaa aagtagtaac agaagaatta cagaaaaata agaagtatac tgtgctttct
     1021 caacaaaaag ttcttgatat tgtgattatc tttattcgtg ttattcaggg attattgatt
     1081 gtgctttcat cacaagctat tgtggtagca gcggttatga ttggtatcat tatttacatc
     1141 aatatcatgc aacgttccaa ggaaataggt gtcatgaaag cagttggtta tcagaatcgt
     1201 gatgtcaaag gaatttttat ttacgaggct atctggattg taggcatcgc cttgctgctg
     1261 gcatttttgg ttgcacaagg ggtgggaagt ttggcgaatg cgattgtaag tcacttttac
     1321 ccatccatca ctaaggtttt tgaattaaat cttttatctg ttttaggaac tctagttttc
     1381 gctctattac ttggttatgt ctcagcctac ttcccggcgc gtaagattag taaaatggat
     1441 cctgtagaat cgttacgcta tgaatagtaa gggattcctt gatttacagt cttaaaaaga
     1501 aactaacata gaaaaacaat ggccatactt gcaattcaac tcaaatagtt gtataatagg
     1561 tttgttgaaa ggtgatttgt agtgattcaa gttactcttt tcacaataaa aatttcatga
     1621 ttttcataag gaggaaatca ctaatggtag ttaaagttgg tattaacggt ttcggacgta
     1681 tcggtcgtct tgctttccgt cgtatccaaa acgtagaagg tgttgaagtt acacgcatca
     1741 acgaccttac agatccagtt atgcttgcac acttgttgaa atacgacaca actcaaggtc
     1801 gtttcgacgg tactgttgaa gttaaagaag gtggatttga agttaacggt aaattcatca
     1861 aagtttctgc tgaacgtgat ccagaacaaa tcgactgggc tactgacggt gtagaaatcg
     1921 ttcttgaagc tactggtttc tttgctaaga aagaagcagc tgaaaaacac cttaaaggtg
     1981 gagctaaaaa agttgttatc actgctcctg gtggaaacga cgttaaaaca gttgtattca
     2041 acactaacca cgacgttctt gacggtactg aaacagttat ctcaggtgct tcatgtacta
     2101 caaactgctt ggctccaatg gctaaagctc ttcaagacaa ctttggtgtt gttgaaggat
     2161 tgatgactac tatccacgct tacactggtg accaaatgat ccttgacgga ccacaccgtg
     2221 gtggtgacct tcgccgtgct cgcgctggtg ctgcaaacat cgttcctaac tcaactggtg
     2281 ctgcaaaagc tatcggtctt gtaatcccag aattgaatgg taaacttgac ggatctgcac
     2341 aacgcgttcc aactccaact ggatcagtta ctgaattggt agcagttctt gaaaagaacg
     2401 ttactgttga tgaagtgaac gcagctatga aagcagcttc aaacgaatca tacggttaca
     2461 cagaagatcc aatcgtatct tcagatatcg taggtatgtc ttacggttca ttgtttgacg
     2521 caactcaaac taaagttctt gacgttgacg gtaaacaatt ggttaaagtt gtatcatggt
     2581 acgacaacga aatgtcatac actgcacaac ttgttcgtac tcttgaatac ttcgcaaaaa
     2641 ttgctaaata attcttgagt tgatagaaag caaggctttg tggtcttgtt tttttatacg
     2701 gaaaaatgga tgacacgatc atccattctt ttttaattct ttttcaaatg tatttgaaag
     2761 ggtagtgaaa gttagcttct ctaaagtaag tgggtgggta aaggaaagtc ggaaggcatg
     2821 aagcataagc cggcttgtct ttgatttact attatagaga gggtctccca ggataggaag
     2881 attatgatgc aaaaggtgca cacgaatctg atgggttcgc cctgtcttta gcttgcaatg
     2941 agccaaggaa gtcttgtttg agaattgctt taatctgctt acatgcgttt cagcatattg
     3001 cccatttttt gcatcaacta ttctttttct acgatcatgg cgatcacgtc caattttgtc
     3061 tctgaaaaca agttcttttc tgttgatatt tccatcaact agagcccaat attctctaga
     3121 aatctctttt ttctccaata agcgattgag aatgggcagg ataaaaggat ttttggcaaa
     3181 gagaactaag ccactggttt ccatgtccag acgatgaacg acatagcagg tttggctaac
     3241 ataggtactg acatggttaa gaagggcaat ttcgtttggt tgattaccat gcgttttcat
     3301 cccctctggt ttgtttacaa taatcaagta ttgatcttga taaacttcct gcactaagtc
     3361 tgggttgccc caagggatcg tcttttgggg ataatcttcc tcgtcaaaag tcaactggca
     3421 aacatctcca ggatttacga tttcgttcca gtggacttct tcttgattta tcaaaatatg
     3481 tttcttgatt ctcaaaaaat gacggatttt tctagggatg aggagttgtt cctcaagtaa
     3541 ttgctttacc gtcatttgag gtagagaggc gggtaatgta aatgtgaatt gcatacagat
     3601 attgtaacaa aaaaagccct atttggatag gaaatagcta aattcttgtc ttcctatgat
     3661 gaagatgata aaataaacgc atgaaattag ataaattatt tgagaaattt ctttctcttt
     3721 ttaaaaaaga aacaagtgaa ctagaggact ctgattctac tatcttacgt cgctctcgta
     3781 gtgatcgaaa aaaaatagcc caagtaggtc cgattcgaaa attctggcgt cgttatcatc
     3841 taacaaagat tatccttata ctaggtttga gtgcaggctt gctagttgga atctatttgt
     3901 ttgctgtagc taagtcgacc aatgtcaatg atttgcaaaa tgccttgaaa actcggactc
     3961 ttatttttga tcgtgaagaa aaagaggctg gtgccttgtc tggtcaaaag ggaacctatg
     4021 ttgagctgac tgatatcagt aaaaacttgc agaatgctgt tattgcgaca gaagaccgtt
     4081 ctttctataa aaatgacggg attaactatg gccgtttctt cttggctatt gtcactgctg
     4141 gacgttcagg tggtggctct accattaccc aacagctggc taaaaacgcc tatttatcac
     4201 aagatcaaac tgttgagaga aaagcgaaag aatttttcct tgccttagaa ttaagcaaaa
     4261 aatatagtaa ggagcaaatt ctaaccatgt accttaacaa cgcttatttt ggaaatggtg
     4321 tgtggggtgt agaagatgcg agtaagaaat actttggagt ttctgcatca gaagtgagtc
     4381 tggatcaagc tgcgactctg gcagggatgc tcaaggggcc ggaactgtat aatcccttga
     4441 attccgtaga agattctact aatcggcgcg atactgtctt gcagaatatg gttgcagcag
     4501 gatatattga taaaaaccaa gaaaccgaag ctgctgaagt tgatatgact tcgcaattgc
     4561 acgataagta tgaaggaaaa atctcagatt accgttaccc ctcttatttt gatgcggtgg
     4621 ttaatgaagc tgtttccaag tataatctaa cagaggaaga gattgtcaat aatggctacc
     4681 gcatttacac agagctggac caaaactacc aagcaaatat gcagattgtt tatgaaaaca
     4741 catcgctatt tccgagggca gaggatggaa cgtttgctca atcaggaagt gtagctctcg
     4801 aaccgaaaac agggggagtt cgtggagttg tcggtcaagt tgctgacaat gataaaactg
     4861 gattccggaa tttcaactat gcaacccaat caaagcgtag tcctggttct acaattaagc
     4921 ctttagttgt ttatacgcca gcagttgaag caggctgggc tttgaataag cagttggata
     4981 accataccat gcagtatgac agctataagg ttgataacta tgcagggatc aaaacgagtc
     5041 gagaagttcc tatgtatcaa gccttggcag aatcgcttaa tctacctgct gttgccactg
     5101 ttaatgattt gggtgtcgac aaggcttttg aggcaggcga aaaattcgga ctcaacatgg
     5161 aaaaggtcga ccgtgttctt ggtgtcgcct tgggaagcgg tgttgaaacc aaccctcttc
     5221 aaatggctca agcatacgct gcctttgcaa atgaaggttt aatgcctgaa gctcatttta
     5281 ttagtagaat tgaaaatgct agtggacaag ttattgcgaa tcataaaaat tcacaaaaac
     5341 gggtgattga taagtctgta gctgacaaga tgaccagtat gatgttgggg actttcacca
     5401 acggtaccgg tattagttca tcgcctgcag actatattat ggcagggaag actggaacaa
     5461 ctgaagcagt ttttaatccg gagtacacaa gtgaccagtg ggtaattggt tatactccgg
     5521 atgtagtgat tagccactgg cttggctttc cgaccactga tgaaaatcac tatctagctg
     5581 gctctacttc aaacggtgca gctcatgtct ttagaaacat tgccaatacc attttacctt
     5641 atacgccagg aagtaccttt acggttgaaa atgcttataa gcaaaatgga attgcaccag
     5701 ccaatacaaa aagacaagta caaaccaatg ataatagcca gacagatgat aatttgtctg
     5761 atattcgagg gcgtgcgcaa agtctagtag atgaggctag ccgggctatc tcagatgcga
     5821 agattaagga aaaggctcaa acaatatggg attcgatagt caatctattt cgctaagatg
     5881 cttgtcaaag cctagctttc ttgttataat ggataagatg gaggcgttat ggcactaaaa
     5941 aaagcaagcc tagcttgtgc ggtttgtggt tcgagaaact attcaatcaa gatcagcgga
     6001 aaccccaagc ctacacgact agaagtaaat aaattttgta agcattgtgg caagtacact
     6061 acacacagag aaacgagata ggagagagcg atgcgtttta ttggagatat ttttagactt
     6121 cttaaagaca caacatggcc aactcgcaag gaaagctgga gagattttcg ttctatcatg
     6181 gaatacacag ctttctttgt agtaattatt tacatttttg accagttgat tgtttcaggt
     6241 ttgattcgat ttattaacat tttttagaag attagtggag ttaattacac tagaaatctt
     6301 ctatttatga aaggaaatat catggatagt tttgataaag ggtggtttgt tttacaaact
     6361 tattctggtt atgaaaataa ggtgaaagaa aatctattac aacgtgcaca aacctacaat
     6421 atgttggata atattctacg cgttgaaatt ccaacacaaa cagtgcaagt tgaaaaaaat
     6481 ggaaagagaa aagaagtaga agaaaatcgt tttccaggtt atgttcttgt agaaatggtc
     6541 atgacagatg aagcttggtt tgttgttcga aacacaccaa atgttacagg atttgtcgga
     6601 tcacacggga acagatcaaa accaactcca ttattggaac aagaaattcg tgacattttg
     6661 gtatctatgg gacaaactgt tcaagaattt gatttcgatg ttgagattgg tcaaactgta
     6721 cgtattattg atggtgcttt tgcagactac actggtaaga ttacagaaat tgataataac
     6781 aaagtgaaaa tgattatctc tatgtttggt aatgacacag ttgcagaagt aaacctaaac
     6841 caaatcgcag aattataacc ctgagagagg ctggagcct
//
LOCUS       DAVKDZ010000094         6819 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000094 DAVKDZ010000000
VERSION     DAVKDZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6819
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.095"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            346..1509
                     /locus_tag="VWZ30_001897"
     CDS             346..1509
                     /locus_tag="VWZ30_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU9335571.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASELENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRSTYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVVAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1506..2183
                     /locus_tag="VWZ30_001898"
     CDS             1506..2183
                     /locus_tag="VWZ30_001898"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU9335572.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLGFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAAMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTMSMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2173..3027
                     /locus_tag="VWZ30_001899"
     CDS             2173..3027
                     /locus_tag="VWZ30_001899"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU9335573.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGTYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3046..4113
                     /locus_tag="VWZ30_001900"
     CDS             3046..4113
                     /locus_tag="VWZ30_001900"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU9335574.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            4123..5289
                     /gene="aroC"
                     /locus_tag="VWZ30_001901"
     CDS             4123..5289
                     /gene="aroC"
                     /locus_tag="VWZ30_001901"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU9335575.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQERGQEIKDYIDKIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5299..6402
                     /locus_tag="VWZ30_001902"
     CDS             5299..6402
                     /locus_tag="VWZ30_001902"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU9335576.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADIIILSLPIKQTISFIKELANLDLKEGVIISDAGSTKSA
                     IVDAAENYLAGKPVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSPDTLEE
                     MKELLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNRETILERIEDFKERLDEVGQAISKGDEEQIWNFFN
                     QAREQRQAMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVVK"
     gene            6413..6751
                     /locus_tag="VWZ30_001903"
     CDS             6413..6751
                     /locus_tag="VWZ30_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEU9335577.1"
                     /translation="MSNIYDSANELSRGLRELPEYKAVKAAKDAIAADAEASKIFTDY
                     LAFQEEIQKLAQTGQMPDASFQDKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
BASE COUNT         1934 a         1291 c         1656 g         1938 t
ORIGIN      
        1 tctctataaa atcaaatttt ccagatatct actgaagctg atttatttct ttatctttct
       61 tctactacct ttgttactaa atttgacaga cacatattgc tgatacaacc tctctaccta
      121 taatctcaca gttaacctga ggctagtttc atagtttgcc atttgatttt cgttgataaa
      181 aagaaaagct cctccaagac ggaagagcat tttttattgt gattcttaca catttacttc
      241 ctagttttgc aggaagtcta gtataccatt ttttaaaaga agagtaaaaa agaaagggta
      301 gtttcttttt tgagaattgt ctaaaagttt gatataatgg tagttatgaa tagaataaga
      361 gtcagcaaaa gggttgaaaa gaagcttgct aaggggctag ttttactaga agccagtgaa
      421 cttgagaatg tcaatcttaa ggatcaggaa gtagaggtgc agggtcagga aggaaacttt
      481 cttgggactg cctatctttc tcagcaaaac aagggcttgg gctggtttgt cagcaaagac
      541 aaggtggcct tcaatcaagc tttctttgaa accttgttta gaaaagccaa agaaaagaga
      601 agcacctact atcaagatga tttgacaact gcctttcgtc tctttaatca agagggagat
      661 ggctttgggg gtctgacagt ggacctttat ggcgactacg ccgttttttc ttggtataac
      721 tcttatgttt atcagattcg tcagactata tcagaagcct ttagacaggt tttccctgag
      781 gttttagggg cttatgagaa aatccgcttt aaggggttgg actatgaatc tgcccatgtt
      841 tatggtcaag aagcacctga ctttttcact gttttagaaa atggtgtcct gtatcaagtc
      901 tttatgaatg atggcttgat gacaggaatt ttcctagacc agcatgaggt tcgcggtagt
      961 ttagttgacg gcttggctat gggtaaatcc ttgctaaata tgttttccta cacagcggct
     1021 ttttcagtag ttgcggccat gggaggagct agccagacaa cttctgttga tctagccaaa
     1081 cgttcacgag aattgtctca agcgcatttt caggcaaatg ggctcagcac agacgagcat
     1141 cgttttatag tcatggatgt ctttgagtat ttcaaatatg ccaaacgcaa agacttgacc
     1201 tacgatgtga ttgtcctaga tccgcctagc tttgctcgga ataaaaaaca aactttctct
     1261 gtggccaagg attatcacaa gttgatttcc cagagtcttg agattttaaa tccgggaggg
     1321 attatcattg ccagtaccaa tgctgccaat gtttcccgtc agaaatttac agaacaaatt
     1381 gataaaggct ttgcaggaag aagttaccag attttaaaca aatatggtct tccagcagat
     1441 tttgcctata ataaaaaaga tgaaagtagt aattacctca aggtgattag tatgaaggtt
     1501 agtaaatgaa attaatcgtt tcagtaatgc caagaagttt agaggaggct caggctctgg
     1561 atgccacgag gtacctggat gccgacatca ttgaatggcg tgccgactat ctgcctaaag
     1621 aagcgatttt gcaggtagct ccagccattt ttgaaaaatt cgcaggccgt gagttggttt
     1681 tcacgctacg aactcgttcc gaaggtggag aaatcgacct ttctccagaa gagtatatcc
     1741 atctaatcaa ggaagttgcg caactctatc aaccagacta tattgatttt gagtactata
     1801 gctacaagga tgtttttgag gaaatgctgg gcttcccaaa tcttgttttg agttaccata
     1861 atttccaaga aacacctgag aacatgatgg aaatcttgtc agagttgacg atcctaaatc
     1921 caaaacttgt caaggttgcg gcgatggctc acacggagca ggatgtctta gacttgatga
     1981 actatacacg aggctttaaa accctcaatc ctgaacagga atatgtgacc atgtctatgg
     2041 gtaaggtggg caaggtctct cgtatcactg cggatgtgac tggttcgagt tggtcttttg
     2101 ccagtctgga tgaggttagt gcccctgggc agatttctct agctagcatg aaaaaaatca
     2161 gggagatttt ggatgaagct tgatggctat acacgtttag ctgcagttgt tgccaatcct
     2221 attaagcatt ctatttctcc cttcatccac aatagagcct ttgaggcgac agctaccaac
     2281 ggtacttacg tggcttggga gattgaagcg agtgacttgg cagaaacagt ggccaatatt
     2341 cgtcgctacc agatgtttgg catcaatctg tccatgccct ataaggagca ggtgattcct
     2401 tatttggatg agctgagcga tgaagcgcgc ttgattggtg cggttaatac ggttgtcaat
     2461 gagaatggca atttaattgg atataataca gatggcaagg gattttttaa gtgcttgcct
     2521 tcttttacaa tttcaggtaa aaagatgacc ctgctgggtg caggtggtgc ggctaaatca
     2581 atcttggcac aggctatttt ggatggcgtc agtcagattt cggtctttgt tcgttccgtt
     2641 tctatggaaa aaacaagacc ttacctagac aagttacagg agcagacagg ctttaaagtg
     2701 gatttgtgtg ctttagaata tgtttctgaa ctgcaagcaa ggattgccga gtcggatttg
     2761 ctagttaatg ccaccagtgt gggcatggat ggccaatcct ctcctgttcc tgaaaacata
     2821 gtcttaccag aaactctatt agttgcagat atcatatacc aaccctttga aacaccgttt
     2881 ttgaaatggg ctagaagtca gggcaatcca gcagtcaatg gtctgggaat gttgctctat
     2941 caagctgcag aagcttttca actgtggaca ggcaaggaaa tgccgacaga agagatttgg
     3001 cagtctttaa cagaaaaata tcaataaaga aagggagact ctcctatgaa aatcagaatc
     3061 gatattcctc atcatcctta tgatattcag attgaaaaag gttgtatggc ccaggctggt
     3121 cagtggttgc gagaactctg gcaaccgcaa aaggtagtca ttgtgacaga taaccatgta
     3181 gcctctctct atgcagagaa ggtcaagctc agcctagaag atgctggctt tcaggtagct
     3241 gtttttgatt tcttagaagg tgaagaaaga aagaatttaa ctactgttca gaaagtctat
     3301 gaatttctag tcaagcaagg tctgactcgt agcgatggaa tcgtggctct tggtggtggc
     3361 gttgttgggg acctggctgg ctttgtagcc tctacctata tgcggggtat tcactttgtt
     3421 cagattccga ctagtttgac agctcaggtt gattcttcta tcggtggaaa gacaggcgtt
     3481 aacacaccat ttgctaaaaa tatggtgggg acctttgccc aaccagatgg ggttctgatt
     3541 gatccacttg ttcttgaaac cctcggaaaa agagagttga ttgaagggat gggtgaagtt
     3601 atcaagtatg gcttgattga ggatccagaa ctgtgggctc tcttgacggg gctgaatggt
     3661 tctgttgaga gcattttgga acatgcagag accttgattg aacattcttg tcaggtgaag
     3721 cgcaagatgg tggttgaaga tgagttggac aatggtattc gtctttacct caattttggc
     3781 cacactattg gccatgccat cgaagcgact gccggttatg gcaaggtcat gcatggagag
     3841 gctgttgcca tgggaatggt acagatttcc aaggttgctg aggaaaaagg cctcatgcca
     3901 gctggcatta cccaatctat cacagagatg tgtcagaaat tcggcttgcc tgttgactat
     3961 gaaaattggg aagttgacaa gctttatcag gctcttactc atgacaagaa agcgcgtggt
     4021 aacaccttga aattggtctt ggtgccagag cttggttcag cgaccattca cccagtttct
     4081 ctggaagaga tgaaagacta cttggtaaaa taaggagagt gtatgagata tttaactgca
     4141 ggagaatcac acggcccccg tctaacagct attattgagg gaattccagc tggacttcca
     4201 ttgacagctg aggatatcaa tgaggacctt agacgccgtc agggtggcta cggtcgtggt
     4261 ggtcgtatga agattgagag tgaccaggtt gtctttactt cgggcgttcg ccacgggaag
     4321 acgacagggg cgcctattac tatggatgtc atcaataagg accaccagaa atggctggac
     4381 atcatgtctg cggaggacat tgaagaccgc cttaaaagca agcggaaaat tactcatcct
     4441 cgcccaggtc atgccgattt ggttgggggg attaagtacc gttttgatga tttgcgaaat
     4501 tctttggagc gttcatcagc tcgtgaaacc accatgcggg tggcagttgg tgcagtagcc
     4561 aaacgcctct tggctgagct ggatatggag attgccaacc atgtcgtggt ctttggtggc
     4621 aaggaaatcg atgttcctga aaatctgaca gtcgctgaaa ttaagcaacg agctgcccag
     4681 tctgaagttt ctattgtcaa ccaagaacga ggacaggaaa tcaaggacta tattgacaaa
     4741 atcaaacgtg atggtgatac catcggtggg gttgtggaga cagtcgtcgg aggcgttcca
     4801 gttggtcttg gttcctatgt ccaatgggat agaaaattgg atgcaagatt ggctcaagct
     4861 gttgtctcca tcaatgcctt taaaggggtg gaatttggtc ttggctttga ggctggttat
     4921 cgtaaaggta gccaagttat ggatgaaatt ctctggtcta aagaagacgg ttatactcgc
     4981 cgtaccaata atctaggtgg ttttgaaggt ggtatgacta atgggcaacc catcgttgtt
     5041 cgtggggtca tgaaacccat tcctactctt tataaacctc ttatgagtgt ggatatcgaa
     5101 acccacgaac cttataaggc aaccgtggag agaagtgatc cgactgctct tccagctgca
     5161 ggaatggtca tggaagcagt tgtagcaacg gttctggcgc aagaaatcct cgaaaaattc
     5221 tcatcagata atcttgagga actaaaagaa gcggtagcca aacaccgaga ctatacaaag
     5281 aactattaag gagttcctat ggcaaaaaca atctatatcg caggtcttgg gttgattgga
     5341 gcctctatgg cacttggtat caaacgcgat catccagatt atgaaatttt aggttataat
     5401 cgtagtcaag cttcgagaga tatcgccttg aaagaaggca tgattgaccg tgcaacggat
     5461 gattttgcta gttttgctcc tttggcagat atcattattc tcagcttgcc aattaaacaa
     5521 actatttcct ttattaagga gttggccaac ttggacttga aagaaggtgt cattatttca
     5581 gatgctggtt cgaccaagtc agctattgtg gatgcagcgg agaactattt ggctggcaag
     5641 cctgttcgtt ttgtaggagc ccatcccatg gctggtagtc acaagacagg ggctgcttcg
     5701 gcagatgtca atctttttga aaatgcttat tatatcttca cgccttcgag tctgacaagt
     5761 cctgacacgc ttgaggaaat gaaggagctg ctttcaggtc ttcatgctcg ctttatcgag
     5821 attgatgcca aggagcatga tcgagtcact tctcagatta gccattttcc tcatattctg
     5881 gcttctagtc tcatggaaca gaccgcggtc tatgctcaag aacatgagat ggcaaggcgc
     5941 tttgcggccg gtggtttccg agatatgact cgaattgcgg aaagtgagcc aggtatgtgg
     6001 acttccattc tcttgtctaa tcgtgagacc attctggagc gaattgagga tttcaaggaa
     6061 cggttggatg aggttggtca ggctatcagc aagggagatg aagagcagat ttggaacttt
     6121 ttcaaccaag cgcgtgagca acgtcaggcc atggaaatcc ataagcgtgg aggtgtggat
     6181 agttcttacg acctctatgt cgacgttccc gatgaagaag atgtcatctt gaggattttg
     6241 gaactactac gtggaacttc cttggtcaat attcatatca acgaggaaaa ccgtgaggat
     6301 attcacggga ttctacaaat ttcatttaaa aatgctcaag acttggaaag agctgagcat
     6361 ctaataacag aaaataccga ctacacagtc gttgtcaaat aaggagaaaa tcatgtcaaa
     6421 tatttacgat agtgcaaacg aacttagtcg cggtctacgc gaattaccag aatacaaggc
     6481 tgtcaaagca gctaaagatg cgattgcagc agatgctgag gcaagcaaaa tctttacaga
     6541 ttatcttgcc tttcaagagg aaattcaaaa actagcacag acaggtcaaa tgccagatgc
     6601 gtccttccaa gataagatgg aaggctttgg caagcagatt caaggaaata gcctcttgtc
     6661 agaattcttt actaagcaac aacaattggc aatttacctt tctgacattg aaaaaattgt
     6721 tttcgaacca gtttcagaat tgctaaaata agaaaataac tatcctaaag tcaggaattt
     6781 ttaaggaatt tctggctttt tatgataaaa taggtaaaa
//
LOCUS       DAVKDZ010000095         6688 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000095 DAVKDZ010000000
VERSION     DAVKDZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6688
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.096"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            147..884
                     /locus_tag="VWZ30_001904"
     CDS             147..884
                     /locus_tag="VWZ30_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9335578.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRHDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEGR"
     gene            881..2572
                     /locus_tag="VWZ30_001905"
     CDS             881..2572
                     /locus_tag="VWZ30_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9335579.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLAVVGTFYYRSSSSAIEATIEGD
                     SQTTISQTSHFIQSYIKKLETTSTSLTQQTDVLAYAENPSQDRVKGIQNLFLTILKAD
                     QDLKTVVLVTKSGQVISTDDTVQMKTSSDMMTEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTLDHQSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     MWTLLWASVTSLFAFLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAEETGAYELR
                     EVTRQFNAMLDQIDQLMADIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFQ
                     DSQRVVQVTKSLATYFRLALNQGKDLISLSDEINHVRQYLFIQKQRYGDKLDYEIAEN
                     PAFDHLVLPKLVLQPLVENALYHGIKEKEGQGHIRVSVQKEDTGLVIRIEDDGVGFQA
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSSPQKGTKVEIYINRIETS"
     gene            2688..3251
                     /locus_tag="VWZ30_001906"
     CDS             2688..3251
                     /locus_tag="VWZ30_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEU9335580.1"
                     /translation="MNETQIQRETRQILEDVLEKANLKQGSIFVLGLSSSEVIGGQIG
                     KESSQEIGELIVETILGILGTRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVLPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPLLREIGHA
                     HVTALASRPKLIGGARAHYPQDSIRKS"
     gene            3273..>6688
                     /locus_tag="VWZ30_001907"
     CDS             3273..>6688
                     /locus_tag="VWZ30_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9335581.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVDKEVI
                     SQEAKDLIQTGNPDGDEVVYGLVYQKAQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASAFLVGAMGLVVLPNAGAVDPVATLAPASREDVVEMEGYRYVGYLSGEILKRLGLD
                     TVLEEASVKSGEVTVVEVETPQSTTNQEQVRPENQAVETEEAPKEQAPRTEESPKEEP
                     KSEVNPTDETLPKVEEEKEVSAEPAPVEEVSGVVESKTDEQAPVKSESQPSDKPAEES
                     KVATPVEQPKIPEQPVQPRTPKESSQEENPTENRGAEETPKQEDEQPAVIETKDEAAN
                     QLVEEPKVETPAIEKQTGPAEEPKVEQAGEPVAPREDEKAPVEPEKQPEAPEEEKAVE
                     KAPKQEESTPDAKVEETVEPKEETKTAKDTQEEGHVDEALAQEQLPEYKVTEGTLVEE
                     TTTELNYKTETTDDPTKYTDEETVLRTGEKGSQVTKTIYKTVEGVKTDQVLSTSTEVT
                     KEHVNQQVSRSTKPIEGTLVEESLEKIPFKEKIEEDDQLKKGSEVVAQEGKEGQKKIT
                     KTFNTIKGVKTEDAPKVIEEVIEVPQDKILKRGTKIFEKPVLTITAVESKDLKRTSDV
                     KYSLENPSKATIKSITLTLKKGDEIVKTLIVSPDNLTASLTDLQYYKDYKLETKMVYD
                     RGEGDEEEVLNEESLRIDLKKIEIKDIKSTKLIKVSENKEEIDDTNFTTIPTDKNNYY
                     LKITTNDNKTNRLAVKSITEETVDGENFYKITSEATDLIQHTKEEQIKNEYTYYIAKP
                     KQKENNVYYDFGELVEAIQNNPEGDYKIGESLNATNIKPNGKSYITKKFKGSLTSTDG
                     NKFAIHNLANPLFNEIENATIKDLIFSNVNINKPNTEQIATLGKDATNTIIENIKITG
                     SIVGSNDVAGVVNNLNNGSKMKNVAVIGKLHTEGKKGWSISGLVNNQRKANIERVYTD
                     VEITGERARGSALVSTLDRGTDPRDVRANGHIKKAVAKGSINLKNNVETGGVISKNWP
                     YGFMEDVISYVKVTNAEKLYGSGDIGDDDFGFPYLSRVVSVAGEATGNVTRKNANRLK
                     EISKEEADKKLEDFGITAKDFEAPTLLVDKLNNNISKDDEYKTTQDYDKT"
BASE COUNT         2341 a         1164 c         1486 g         1697 t
ORIGIN      
        1 tacaacctca aagcggtatt ttgagaaacc tgtggctatc ttcctagttt actttttgat
       61 tttcattgag tataagaggt atttcctaag cagttagagg agaattttgc tatactgata
      121 ctagtaagtg acataaggag agaagcatga cctacacaat cttaatcgta gaagatgaat
      181 atctggtaag acaaggtttg actaaactgg tcaatgtagc agcctacgat atggaaatca
      241 tcggtcaggc tgaaaatgga aggcaggctt gggaattgat ccaaaagcag gtgccagata
      301 tcattttaac cgatatcaac atgcctcatc taaatggcat ccagttggcc agtctggtac
      361 gagaaaccta tcctcaggtt catttggtct ttttaacagg ttacgatgat tttgattatg
      421 ccttgtctgc tgtcaaacta ggtgtggacg actacctgct caaacccttt tctcgtcatg
      481 atattgagga aatgttgggg aaaatcaaac aaaaactaga caaggaagag aaagaagagc
      541 agttacaaga tttattgact aataggtttg aaggaaacat ggcccagaaa atccagtctc
      601 atctggctga tagccaattt agtttaaagt ctttagccag tgacttaggt tttagtccga
      661 cctatctgag ttccttgatt aagaaagagt tgggcttgcc ttttcaggat tatctggtga
      721 gagaacgtgt taaacaagct aagctcttgc ttttaactac agatctgaag atttatgaga
      781 ttgcagagaa ggttggtttt gaagatatga actattttac ccaacgtttt aagcagattg
      841 caggtgtgac acctcgtcag tttaagaagg gagaaggccg atgaagcgtt cttctctttt
      901 agttagaatg gttatttcca tctttctggt ctttctcatt ctcctagctg tggttgggac
      961 tttctactat cgatctagtt catcagccat tgaggctacc attgaaggcg atagccaaac
     1021 gactatcagt cagactagcc actttatcca gtcttatatc aaaaaattag aaacgacctc
     1081 gaccagtttg acccagcaga cggatgtcct agcctatgct gagaatccta gccaagacag
     1141 agtaaaggga atccaaaatc tgtttttgac catcttgaag gcggatcagg acttgaaaac
     1201 ggtggtactg gtaaccaagt caggtcaggt catttctaca gatgacactg tgcagatgaa
     1261 aacatcctca gatatgatga ctgaggattg gtaccaaaag gctattcatc agggagccat
     1321 gcccgttttg accccagcgc gtaagtcaga cagccaatgg gtcatttctg ttactcaaga
     1381 acttgttgat gcgaagggag ccaatctggg tgtgcttcgt ttggatattt cttatgaaac
     1441 tctggaagcc tatctcaatc aactccagtt ggggcagcag ggctttgcct tcattatcaa
     1501 tgaaaaccat gaatttgtct accatcctca acacacagtt tatagttcgt ctagcaaaat
     1561 ggaggctatg aaaccctaca tcgagacagg tcagggctat acactagacc accaatccta
     1621 cgtcagtcag gaaaagattg caggaactga ttggacggtg cttggcgtgt catcattgga
     1681 aaagttagat caggttcgga gtcaactcat gtggaccttg ctttgggcta gtgtcacatc
     1741 tctttttgcc tttctctgct tggtgtggtt cagtctcaaa cgctggattg ctcctttgaa
     1801 ggatctgaga gaaaccatgc tggaaattgc ctctggtgct caaaatcttc gcgccgaaga
     1861 aactggtgcc tatgaactga gagaagtgac tcgtcagttc aatgccatgc tggatcagat
     1921 tgatcaactg atggcagata ttcgtagcca ggaagaaacg acccgtcagt accaacttca
     1981 agccctatca agccagatta acccccattt cctctataac accttggata ccatcatctg
     2041 gatggctgaa tttcaggaca gtcagcgagt ggttcaggtg accaagtcct tggcaaccta
     2101 tttccgcttg gcgctcaatc aaggcaagga cttgatttcc ctgtctgacg aaatcaatca
     2161 tgtccgccag tatctcttta tccagaaaca acgctatgga gataagttag actatgaaat
     2221 tgctgaaaat cctgcctttg atcatctagt tttgcctaag ctggtgttgc aaccccttgt
     2281 agagaatgct ctttaccatg gcatcaagga aaaggaaggt cagggacata ttagggtctc
     2341 tgtccagaaa gaggatacag gcttggtcat ccgtattgag gatgatggcg ttggcttcca
     2401 agctgctggc gatagtagtc aaagtcaact caaacgtggg ggagttggtc ttcaaaatgt
     2461 cgaccaacgg ctcaaacttc attttggagc caattaccag atgaagattg attctagccc
     2521 ccaaaaaggg acgaaagtgg aaatatacat aaatagaata gaaactagct aactcccagt
     2581 caattctggg agttttatgc gtaattgggc aagctttcca ggttgtctat cttgctaaaa
     2641 acaagaaaaa acggtatgat agaagagtga taaaagaggt ggcagagatg aatgagacac
     2701 agattcaaag agaaacacgt cagatattag aagatgtttt agagaaggcc aatttgaaac
     2761 aaggctccat ctttgtttta ggcctttctt ctagtgaggt gataggtggt cagattggga
     2821 aagaatccag tcaagagatt ggcgagctga tagtggagac tatcttgggt attctaggta
     2881 ctagaggtat ccatttagct gttcaaggtt gtgaacatgt caatcgggcc ctcgttgttg
     2941 aacgtcaggt ggcagagcag tttggtctgg aaattgtcag tgtccttcct acacttcatg
     3001 caggaggttc ggggcagtta gcagccttca agttcatgca ggatccagtt gaggttgaat
     3061 ttatcaaggc tcatgctgga ttggatatcg gagacactgc aattggcatg catgtcaagc
     3121 atgttcaggt tccgattcgc cctcttttga gagagattgg tcatgcccac gtaacggcac
     3181 tggctagtcg tccaaaatta attggaggtg cgcgtgcgca ctatccgcaa gattctatta
     3241 gaaagtcgta agaatgaata aaggagatga aagtgtttaa aaaagatcgt ttttcaattc
     3301 gtaagattaa gggagttgta ggttctgtat ttcttggaag ccttttaatg gctccttctg
     3361 tagtggacgc agccacctat cactatgtag ataaagaggt tatttcacaa gaagctaaag
     3421 atttaatcca gacaggaaat cctgatggag atgaagttgt ctatggtttg gtgtatcaaa
     3481 aagctcagtt gcctcaaaca ggcacagaag catctgtttt gacagctttt ggtttgctga
     3541 ctgttgggag cttgctttta atctacaaga gaaagaaaat tgctagcgcc tttctagttg
     3601 gagctatggg attggtagtc cttcctaatg caggggctgt agacccagtt gcgaccctag
     3661 caccggctag tcgagaggat gttgttgaaa tggagggcta tcgctatgtg ggttatctat
     3721 caggtgaaat cctcaaaagg cttggtttgg acactgtttt agaagaagct tcagttaaat
     3781 ctggagaggt gactgtagtc gaagttgaga ctcctcaatc aacaacaaat caggagcaag
     3841 tgaggccaga aaaccaagca gtagagacag aggaagctcc aaaagaacaa gcacctagaa
     3901 cagaagaaag tccaaaagaa gaaccaaaat cggaggtaaa tcctactgac gaaacccttc
     3961 ctaaagtaga agaggaaaaa gaagtttcag cagaaccagc tccagttgaa gaagtaagtg
     4021 gagtagttga gtcaaaaaca gatgaacaag caccagtcaa atcggaaagt caaccatcag
     4081 acaaaccagc tgaggaatca aaagttgcaa ctccagtaga acaaccaaaa attccagaac
     4141 aacccgttca accaaggaca ccaaaagaat catcacaaga agaaaatcct acagaaaata
     4201 ggggagcgga agagacaccg aaacaagaag atgaacagcc agcagtaata gaaacaaaag
     4261 atgaggctgc taatcaactt gtcgaagaac caaaagttga aacgcctgct atagaaaaac
     4321 aaacgggacc agctgaggaa ccaaaagttg aacaagcagg tgaaccagtc gcgccaaggg
     4381 aagacgaaaa ggcaccagtc gagccagaaa agcaaccaga agctcctgaa gaagagaagg
     4441 ctgtagaaaa agccccaaaa caagaagaat caactccaga tgccaaggtt gaagaaactg
     4501 tagaaccaaa agaggagacc aaaacagcta aagatacgca agaagaaggt cacgtggatg
     4561 aggctcttgc tcaagaacaa ttacctgagt ataaagtgac tgaaggaaca cttgttgaag
     4621 aaacaactac agaacttaac tataaaactg aaacgactga tgatccaact aagtatacag
     4681 atgaagaaac tgtcctacga actggtgaaa aaggaagtca agttacaaaa acaatctata
     4741 aaacagtaga aggtgttaaa actgaccaag ttctttcaac cagtacggaa gtgaccaagg
     4801 agcatgtaaa tcaacaggta agccgtagta caaaaccaat tgaagggact cttgtagaag
     4861 aaagtcttga aaagattcca tttaaagaaa agattgaaga agatgatcaa ctgaaaaaag
     4921 gttcagaagt cgtagctcaa gaaggcaaag aaggtcagaa aaaaattact aagaccttta
     4981 ataccatcaa aggagttaaa acagaagacg ctccaaaagt tatagaggaa gtcattgagg
     5041 tccctcaaga caaaattttg aaacgtggta ccaaaatttt tgaaaaacca gtattgacta
     5101 taacggcggt tgagtctaag gatttgaagc gtacttcaga tgttaaatac agtctagaaa
     5161 atccaagcaa ggcgaccatt aaatccatca ccttaactct gaaaaaaggt gatgagattg
     5221 tgaaaacatt gattgtttca cctgacaact tgacagccag cctgacagat ttgcaatact
     5281 acaaggatta taaacttgaa acaaagatgg tttatgaccg tggtgagggt gatgaggaag
     5341 aagttctgaa tgaagaatct ttaagaattg accttaagaa aattgagatt aaagatatta
     5401 agtctacgaa actaataaaa gttagtgaaa ataaagaaga aatagatgat actaatttca
     5461 caacaattcc tacagataaa aataattatt atttaaaaat cacaacaaat gataataaga
     5521 caaatagatt agcagtaaaa tctattacag aagaaacagt agatggagaa aacttttata
     5581 agattacttc agaagcaact gatttaattc aacatactaa agaagaacaa atcaaaaatg
     5641 aatatactta ttatatagca aaaccaaaac aaaaagaaaa caatgtttac tatgattttg
     5701 gagaattagt tgaagctatt caaaacaatc cagaaggaga ttataaaatc ggagaaagtt
     5761 tgaacgctac taatattaaa ccaaatggaa aatcttatat tacaaagaaa ttcaaaggaa
     5821 gtttaacaag tactgatggg aataagtttg ctatacataa cttagctaat ccattattta
     5881 atgaaataga aaatgctaca ataaaagatt tgattttttc taatgtaaac ataaacaaac
     5941 caaacacaga acaaatagca acattaggaa aggatgcaac aaatacaata attgaaaaca
     6001 ttaaaattac tggaagtatt gtaggtagta atgatgtagc tggtgttgtt aataatttaa
     6061 ataacggtag caagatgaaa aatgttgctg ttataggaaa attacacaca gaagggaaaa
     6121 aaggttggag tatatctggt ttagttaata accagagaaa agcaaacatt gaaagagttt
     6181 atacagatgt agagattaca ggagaaagag ccagaggttc agctttagtt tcaacattag
     6241 acagaggtac ggatccaagg gatgtgcgag ctaatggaca tattaaaaaa gctgtagcaa
     6301 aaggatcaat aaacttaaaa aacaatgtgg aaacaggtgg agttatttct aaaaactggc
     6361 cttatggatt tatggaagat gtaattagtt atgtgaaagt aactaatgct gaaaaactgt
     6421 atggttcagg agacattgga gatgatgact ttggattccc ttatttaagt agagttgtta
     6481 gcgtagctgg agaagcaact ggtaatgtaa caagaaaaaa tgcgaataga cttaaagaga
     6541 tttctaaaga agaagctgat aaaaaattag aagattttgg tattactgcc aaagattttg
     6601 aggctccaac attattagta gataagttga ataataacat atcaaaagat gatgaatata
     6661 aaacaacaca agattatgac aaaacaaa
//
LOCUS       DAVKDZ010000096         6679 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000096 DAVKDZ010000000
VERSION     DAVKDZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.097"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(127..300)
                     /locus_tag="VWZ30_001908"
     CDS             complement(127..300)
                     /locus_tag="VWZ30_001908"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335582.1"
                     /translation="MKMSLIYSSAKKDFLYWNVLIFIMELPNDVKVQFYELRKKVQSF
                     NQLSKRFGMDVSG"
     gene            complement(461..532)
                     /locus_tag="VWZ30_001909"
     tRNA            complement(461..532)
                     /locus_tag="VWZ30_001909"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(497..499),aa:Arg,seq:cct)
     gene            complement(574..921)
                     /gene="rplS"
                     /locus_tag="VWZ30_001910"
     CDS             complement(574..921)
                     /gene="rplS"
                     /locus_tag="VWZ30_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU9335583.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(1040..1369)
                     /gene="crcB"
                     /locus_tag="VWZ30_001911"
     CDS             complement(1040..1369)
                     /gene="crcB"
                     /locus_tag="VWZ30_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9335584.1"
                     /translation="MVIVYLAIACGFGALVRYLFSRYNQASKMPLGTLIANLLGCLLI
                     GLFYIHVESKEVYAILATGFCGSLTTFSTLNDELQRLLSDKKVFYSYLILTYLGGLVA
                     IFLGILL"
     gene            complement(1363..1737)
                     /gene="crcB"
                     /locus_tag="VWZ30_001912"
     CDS             complement(1363..1737)
                     /gene="crcB"
                     /locus_tag="VWZ30_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9335585.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLV
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(1734..2000)
                     /locus_tag="VWZ30_001913"
     CDS             complement(1734..2000)
                     /locus_tag="VWZ30_001913"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU9335586.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREAVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(2116..2559)
                     /locus_tag="VWZ30_001914"
     CDS             complement(2116..2559)
                     /locus_tag="VWZ30_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEU9335587.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            2663..3598
                     /locus_tag="VWZ30_001915"
     CDS             2663..3598
                     /locus_tag="VWZ30_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU9335588.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNITDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKVHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3652..3936
                     /locus_tag="VWZ30_001916"
     CDS             3652..3936
                     /locus_tag="VWZ30_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU9335589.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(4009..4961)
                     /locus_tag="VWZ30_001917"
                     /pseudo
     CDS             complement(4009..4961)
                     /locus_tag="VWZ30_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            5308..6519
                     /locus_tag="VWZ30_001918"
     CDS             5308..6519
                     /locus_tag="VWZ30_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein C"
                     /protein_id="HEU9335590.1"
                     /translation="MNNKTKKFTLATVLVLSLLGAGTLTSQSVVANDRLVSTQEINGQ
                     SYNILNSEVTSTSSGTTLVGIEGTFLTPDKQAILDRINAIRKEAADEGLVTSYVPIKW
                     STALEKTAFVRAAEASITIDHTRLSSKDIWTSWPAGNFSLSENLAWNYNGFMKAIQQW
                     YDEKADYVKSRSGVSVAGQTGHYKSLIDPKLTYMGLAAFENPVTQNGWITVAQSFGTA
                     SGGSEELAGSYGKAIQYTEANSSQIQTFATKANLFDKDLKAIRTHKTKNENTNSSQKN
                     EFNHSQYFGSWIQSNGRWWFKHNDGSYTSNGWEKINRTWYRFDNSGWMQTGWVKDGSW
                     YYLDGSGAMKTSWVKDNGSWYYLQDSGAMKTGWMKVSGKWYYAYSSGALAINTTTPDG
                     YRVNYNGEWVG"
BASE COUNT         2073 a         1379 c         1240 g         1987 t
ORIGIN      
        1 aatacatttg ttattatata ggcttttgtt gatttatttc ttgttttaat tcagtggaat
       61 agtaacgatt cttccctttt ttaacgctct ctgttcaata acgatcaatt caccttgtca
      121 cataccttag ccagaaacat ccataccaaa tctttttgaa agctgattaa agctttgtac
      181 tttctttcta agttcataga actgaacctt aacgtcattt ggcaattcca taataaaaat
      241 aaggacattc caataaagga agtccttttt agcactacta taaatcagag acattttcat
      301 gatccatttt attctataat cattgaccaa aatataattt ttggtcaaaa tttggtcaaa
      361 aaaattaaaa atgctaaatt atttttaaaa atgaacttga gaaaattgcc ttcaaataaa
      421 cattttgaat tttctttgtc tttctttaca ttactaaaaa tgccccctgc aggaatcgaa
      481 cctgcaacta ctccttagga gggagttgtt atatccattg aactaaggga gctagataaa
      541 aactctgctg aaaagcagag ttttttagtc gaattaacga cggatttctt tgatacgagc
      601 tgctttacct tgaagagcac gcaagtagta caatttcgca cgacgtactt taccgtaacg
      661 aacaacttcg attttttcaa cacgtggagt gtggattggg aagatacgct caacacctac
      721 accgttagag attttacgaa ctgtgtagtt ttctgagatt ccagcacctt tacgtgcgat
      781 aacaacacct tcaaaaatct ggatacgttc acggttacct tcgacaactt tcgcgtgtac
      841 acgaacagtg tcaccaggac ggaatgatgg gatatctgta cgaagttgac cttcagtcaa
      901 gctttggatt aatggattca ttttattctc ctatctttgt caatcttgag gaatcttcct
      961 cagcggataa actgtatttt tgtgcgtcca ttacacacaa gatacagttt accaaatttc
     1021 taaaaaaaag taaagaaatt tatagcagaa ttcctaaaaa aatcgcaacc aaaccaccta
     1081 ggtaggtcaa aatcaggtaa gaataaaata ccttcttatc gcttagcaac ctttggagtt
     1141 cgtcattcaa agtcgaaaaa gttgtcaaac ttccacaaaa tcctgttgct agaatagcat
     1201 agacctcctt agactctaca tgaatgtaga ataatccaat caacaaacaa cctagaagat
     1261 tggctatgag cgttcccaag ggcattttag aagcttgatt gtagcgggaa aataaatagc
     1321 gtactaaagc tccgaaacca caagcaattg caagatagac gattaccatt tcttcctccc
     1381 caaatagtaa gctaaaagca ggcctccacc gatactcaaa agcaaataca ggactaaact
     1441 aagataacgc cctgtatcaa gcagcttcac agtatcaagc attaggctgg aaaaagttgt
     1501 taaacctccg caaaaacccg tccccagtgc taaaatcaag cccttactag tccccttata
     1561 gaccagatag cccttgacaa gataaatcaa gcagaaaatt cccagatagt tgacaaagag
     1621 agtgccccaa gggaaatctg gactggctgg taaccaagtg gaaactaggt agcggacaag
     1681 tccgcccaac atagcagcta gaaaaatccc tagcggataa aattgttctt ttttcatttg
     1741 atattttgat cctgataatc acgcgaacgt ttgagtatgt ccgaaaaagt cgcgacaata
     1801 gtctcctgat agcgcttgtc ctctacacga cttctaacct tttcaaaaat aactgcttct
     1861 ctcttagtat ctaaaatcgg tttccctgaa gccttcttat aagcgacaac tccttcaact
     1921 aagtgcattc gttcttctaa cagtttgaca atttggtcgt cgatttgatc gatttcttga
     1981 cgaataatat ctaaatccat acagtctcct cctttatttg aattattgta tcaaaaagcc
     2041 atcaattagg ctagtaaaat cccaactctc gataagaaaa atgcactgat tgattacatc
     2101 agcgcacttt tatgcttatc ctactttagc agccaattct tctgcgaatt gttccaagcg
     2161 ttcaatatct tcttcctcgg cagaaagatc aactttaaca cactctgaac ctttttctgc
     2221 tcctgttgac acaaagacac ggtcaaagtc atcaacagcc ttacaaaatt catcgtagaa
     2281 ggtatctcct gagccgacca ctccgtagat tttgccattc aagttgagat ctgctaggtc
     2341 ttcgtagaag tccatcatct catctggcaa ttcaccatca ccataagtat aggtcgcaac
     2401 gatagcgatg tctgcttcca agaagtctga agcgtcaaca gttgtacatt catcaacatc
     2461 aacatccaag cccaagtcac gtaatttgtc tgctacaata tctgcaattt cttcggtatt
     2521 accggtcata ctggcaaata caatttttgc taatgccata tcgtcctcct caatttatct
     2581 ttctccatta tatcacatct ttttcatttt aaaaataaaa attcttgtgc tacaatgaaa
     2641 tgagaaagaa ttgaggttat ttatggagat ttgccaacaa attttagaaa aaattaaaga
     2701 atacgacacc attatcattc atcgtcatat gaaaccagac cctgatgcct tgggaagtca
     2761 ggtgggattg aaagccttgc tagaacatca tttcccagaa aaaaccatca aagccgtcgg
     2821 ttttgatgaa ccaactctta cttggatggc tgagatggat cttgttgaag atagagccta
     2881 ccaaggcgca cttgtcatcg tctgtgatac agctaatact gctcgtatcg atgataagcg
     2941 ctatagtcaa ggtgattttc tcattaagat tgaccaccat ccaaatgatg atgtatacgg
     3001 tgacctgtct tgggtcgata ctagttcaag tagcgctagc gagatgatta ccctatttgc
     3061 ccaaacaacc caactagcct tggcagatcg cgatgctgag ttgctctttg caggaattgt
     3121 cggtgataca ggtcgcttcc tctacccttc taccactgca cggactcttc gcctggctgc
     3181 ttatttgaga gaacataact ttgactttgc ggctctcact cgcaaaatgg acactatgag
     3241 ctacaaaatt gctaaactgc aaggctacat ctacgaccat ctggaagtgg atgaaaatgg
     3301 tgctgctcgc gttatcctga gtcagaaaat cttgaaacaa tacaatataa ccgatgctga
     3361 aactgcggcc attgtaggtg cacctggacg cattgacaga gtgagtctct ggggaatttt
     3421 tgtcgaacag gctgatggcc actaccgagt tcgcttacgc agtaaagtcc atcctatcaa
     3481 tgaaattgcc aaggagcatg atggtggagg ccaccctcta gcaagtggtg ctaattccta
     3541 tagcctagaa gaaaacgaaa tcatctacca aaagttaaaa aacttgctta aaaactgata
     3601 aaatacttgc caaacttttc agaatctgat agactagtat agtaacaatc tatggctcgc
     3661 aaagagacca tggcagaaag gaaatattgc aaaatgaaaa aagatatcca tccagaatat
     3721 cgcccagttg tcttcatgga cacaactact ggttaccaat tccttagcgg ttcaacaaaa
     3781 cgctctaacg aaacagttga gttcgaaggc gaaacttacc cattgatccg tgtggaaatt
     3841 tcatcagact cacacccatt ctacactgga cgtcaaaagt tcactcaagc agatggacgc
     3901 gtggatcgtt tcaacaaaaa atacggtctc aaataatgat aagagaacag ttttggctgt
     3961 tcttttttgt ttcttgaaat caacgccaaa tttcaagttc aagttagcca tcaagaagtc
     4021 ttctctgggt gatttgtagt tcaagcattt tttaggatag ttattaatcc aattttcgat
     4081 gaatgcgact tctttgggag tcgttttctt agttccctta ggtaaccatc tacgaatgag
     4141 cctgttgtga ttctcattag ttcccctttc ccaagaggca taggggtgcg cataatagat
     4201 atgttcctca gaaaatacat cagacaagcg gttaaattct gtaccattat ctgccgtgat
     4261 ggaaagaatt tgttgttgtt ttaagatgag ttttaaagcc tgattgaccg cctcagcact
     4321 tttatttgga atcaatcgga taatttgatg cctagtcctt cgatcagtta agaccagtaa
     4381 acaataattt ttcactcttg taagcagaac cgtatcaatc tcataatgcc cattctccaa
     4441 tcgcagattg atagcttcag gacgctgttc gatagattgg attgaccagg ggggttaaaa
     4501 ttggtgctag cttgtttctt aacagatttt ccttttctag gatagagcag atcctgtttt
     4561 gacaatccta attttccatg atggatccag tagtagatgg taaaaattcc cacgttaacg
     4621 cccttagcct tgaccatcat ttcaggagaa aacttttggt tatgatagtg gagaatcttt
     4681 tccttcagct ccttgttcaa tcttgatttc ttgatcgagc gcttgcgatt attttcataa
     4741 gactgttgag catagtcggc agaataaacc tctttaaagc gcccttttcc aagacattgt
     4801 cggattgtcc cacgcttgat ttcagtatgg atagtttgag gagcttttcc aagcagagag
     4861 gcgatttctc tatttgattt cccttccttt ttccatcgtt cgattaagcg acgactatcg
     4921 attgtcaaat gtttggcttt tgtagtataa tggttttgca tctctgtgcc tttcttgtgt
     4981 ttgtggttga acaacaagta taacacagag gtgttttctt atgcctagaa gagctttcat
     5041 ttgacaagag gaacccttga agcagttttg tcagctaaac caaggctaat cttatccttg
     5101 tcttgtcagc ctaacagctc caagcctctt atcaaatgaa atcgagtact agctataagc
     5161 tagttaaaac atctaatttt taggaggcct gggtggctaa cttcattata gaactttcaa
     5221 tttaaaataa atatagtccc tagctatacc gaattttagt ttactctacc cagtaaagat
     5281 gttataataa aaataggagg acacaaaatg aacaataaaa ctaaaaagtt cacacttgct
     5341 acagttttag tactttcctt gttaggagct ggaactctta catcacaatc agtagtcgct
     5401 aatgataggc tggtttccac tcaagaaatc aatggtcaat cctacaatat cttgaactct
     5461 gaggttacta gcacttcttc tggtactact ctagttggga ttgaaggaac tttcttaacg
     5521 ccagataaac aggctatctt agaccgtatc aatgccattc gaaaagaggc ggcagatgaa
     5581 ggtctagtta ctagctatgt tcccatcaaa tggtcaactg cactagaaaa aactgccttt
     5641 gttcgtgcag cagaagcttc gatcaccatt gatcacacac gcttgtctag caaggatatt
     5701 tggacttcct ggcctgcagg aaatttctcc ctttcagaaa atttggcttg gaactacaat
     5761 ggatttatga aggccatcca gcaatggtat gatgaaaaag ccgattatgt taaatctcgt
     5821 agtggtgtca gtgttgcagg tcaaactgga cactataaat ccttaattga tccaaaactg
     5881 acctatatgg ggcttgcagc ctttgaaaac cctgtcactc aaaatggatg gattaccgtt
     5941 gcacaaagtt tcggaacagc atctggtggc tctgaagaac tagctggtag ttatggaaaa
     6001 gccattcaat acacagaagc aaattcttct cagattcaaa catttgcgac taaggcaaat
     6061 ctttttgaca aagacttaaa ggctatcaga actcataaaa ctaaaaatga aaatacaaat
     6121 tcaagtcaga aaaatgagtt caatcattcc caatacttcg gaagttggat tcaatcaaac
     6181 ggacgttggt ggtttaaaca caatgacggc tcatacacga gtaatggatg ggaaaaaata
     6241 aatagaactt ggtatcgctt tgacaactct ggttggatgc aaacaggctg ggtaaaagac
     6301 ggtagttggt actatctcga cggctcaggt gcgatgaaga caagttgggt caaagataat
     6361 ggtagctggt actaccttca agattcaggt gctatgaaaa caggctggat gaaagtatct
     6421 ggcaaatggt actatgccta tagctctggt gccctagcaa tcaatacaac tacacctgac
     6481 ggttatcgtg tcaattacaa tggggaatgg gtgggataat ttaactacaa caaaaagact
     6541 ctatactatc tcacagtgag taatcttcac tttcagatag tatagagatt tggctctttg
     6601 tcaactgtag tgggttgaaa aaaagctaag cttgagaaag gacaaatttt gtcctttctt
     6661 ttttgatgtt cagagcgat
//
LOCUS       DAVKDZ010000097         6403 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000097 DAVKDZ010000000
VERSION     DAVKDZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.098"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            275..796
                     /gene="pyrR"
                     /locus_tag="VWZ30_001919"
     CDS             275..796
                     /gene="pyrR"
                     /locus_tag="VWZ30_001919"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU9335591.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVADREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            815..1738
                     /locus_tag="VWZ30_001920"
     CDS             815..1738
                     /locus_tag="VWZ30_001920"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU9335592.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPCEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIVNGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            1788..2867
                     /gene="carA"
                     /locus_tag="VWZ30_001921"
     CDS             1788..2867
                     /gene="carA"
                     /locus_tag="VWZ30_001921"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU9335593.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            3179..6355
                     /gene="carB"
                     /locus_tag="VWZ30_001922"
     CDS             3179..6355
                     /gene="carB"
                     /locus_tag="VWZ30_001922"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU9335594.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAIEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLETGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGLENESIKSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         1853 a         1327 c         1446 g         1777 t
ORIGIN      
        1 cttcacaact gtcaaagtcc cgtgtgacca gtacccacaa ttatatgatt ttagcaattt
       61 gtaagatgct acattgaaac tttttgcttg attttaagca tgctttgtgc tatagtaaat
      121 gataactaaa tattccttta aacctgtccc gtgaggcagg caaggagcgc gataaagtag
      181 aagggtgaag tctatactgt gtgcagtagg gctcgcttgg ctataaattt caagtctcct
      241 tgtttaagga gacttttctt tttggaggtt tgtcatgaaa acaaaagaag ttgtagacga
      301 attgaccgtc aaacgagcga ttacgcgtat tacttatgag attatcgaac gcaacaaaga
      361 tttgaataag atcgtcttgg ctggtattaa aactcgtggt gtctttattg cccaccgaat
      421 ccaagaacgt ttgaagcagt tagaaaatct ttcagttcct gttgtggaat tggatactaa
      481 acctttccgt gatgatgtta aaagtggaga agatacttct ttggtttctg tcgatgtggc
      541 agaccgcgaa gttatcttgg tggatgatgt gctctataca ggtcgtacca tccgtgctgc
      601 tattgataat attgtcggtc atggtcgtcc tgcgcgtgtg agtttagcag ttctagtcga
      661 tcgtggacat agagaattgc caatccgtcc agattacgtt ggaaaaaata tcccaaccag
      721 tcgttctgaa gaaatcatcg tagagatgac agaacttgat gaccaagaca gagttctgat
      781 tactgaagaa gcttagaaag ttaaaggagt agccatgtca gaaaatcaac aagcattgaa
      841 ccatgtggtg tccatggaag acctcactgt cgatcaagtg atgaaattga tcaagcgagg
      901 aattgagttt aaaaatggag cccagcttcc ctgtgaagac catccgattg tttccaatct
      961 cttctttgag gattctacac ggacacataa gtcctttgaa gtcgcagaga ttaaacttgg
     1021 attggaacga cttgactttg atgtgaagac tagttcggtt aataagggtg agacacttta
     1081 tgacaccatt ttgactctgt ctgctttagg agtggatgtc tgtgtgattc gccatccaga
     1141 ggtcgactac tatagagaat tgattgctag tccaacgatt acgacttcca tcgtcaatgg
     1201 tggagatggt tcgggccaac accctagcca gagcttgctt gatttgatga ccatttatga
     1261 ggaatttggc cactttgagg gtcttaaagt tgctattgca ggtgacttgg atcactcacg
     1321 cgttgccaaa tccaatatgc agattttgaa acgcttggga gctgaactct ttttcgctgg
     1381 acctgaggaa tggagaagtc aagagtttgc agactatgga cagtttgtaa ccattgatga
     1441 aatcattgat caggttgatg tcatgatgtt tctccgtgtg caacacgaac gccatgatag
     1501 tggagctgta ttttcaaaag aagactacca tgcccaacat ggcttgactc aagaacgtta
     1561 cgatcgtttg aaagaaacag caatcctcat gcacccagct ccaatcaatc gtgatgtaga
     1621 aatagcagac cacttggttg aagcaccaaa atcacggatt gtccaacaaa tgaccaatgg
     1681 tgtctttgtt cgaatggcaa tcttagaatc cgtactagcg agtagaaacg ccaactaaaa
     1741 cacaagacgt taaaagacgc cagtgagcgt ccacgagagg tgaaaatatg acaaaaagaa
     1801 ttctagtatt agaagatggc acagtttttg aaggcaaggc cttcggagca gatattgatg
     1861 taacaggcga aatcgtcttt aatacaggaa tgaccggcta ccaagaatcc attacagacc
     1921 agtcttataa tggacaaatc ttgaccttta cttatccttt ggtaggaaat tatggtatta
     1981 atcgtgatga ttatgaatcc attattccaa cttgtaaggg agtcgttgtt tttgaagaag
     2041 cgcgtagagc tagcaactgg cgcaaccaaa tgaccttgga tgaatttttg aaagccaaga
     2101 aaattccagg gatttcagga attgatacgc gtgctcttac caagattatc cgtaagcatg
     2161 gtactatgcg tgcaaccttg acccatgttg gggacagtat ggaccatgtg acggaccagc
     2221 tccaagcaac agttttaccg acagacaata tcaaacaggt ttctactaaa acgtcttatc
     2281 cagctccagg agttggtttg agcgtggtgc tagtggactt tggtctcaag cactcaatcc
     2341 tacgtgaact ttctaaacgc aactgtaacg tgacagttgt tccttattcg acaacggctg
     2401 aagaaattct ccatctcaat cctgacggag ttatgttgtc aaatggtcca ggtaacccag
     2461 aagacgttcc acaagcactc gacatgattc gcggtgtaca aggaaaaata ccaatctttg
     2521 gtatttgtat gggacaccaa ctctttgcaa tggcaaacgg ggctaagacc tataagatga
     2581 agtttggcca ccgtggattc aaccatgcgg tacgtgaaat cgcaacagga cgagtagact
     2641 tcaccagcca gaaccatggt tatgcagtca gccgcgagga tttgccagag cacttgatta
     2701 ttacacacga agaaatcaat gacaagtcag tggaaggtgt gcgccacaga taccaacctg
     2761 ctttctctgt tcaataccac ccagatgcag ctcctgggcc acacgacgct agctacctat
     2821 ttgacgagtt tatcgagatg atggaagttt ttaaacaatc aaactaagaa cgagtaaaag
     2881 ctcgtcggag aatttatgta actgaacacg gacggaaagc tcagaattta gagaaaccaa
     2941 cttggattgt caatccttcc ttgattttct aaaattcaat cgctttctat tcgtcctttg
     3001 tatcttattt aatgtcgccc tgagaggcga cgaatagaaa ggagaagggt gctccgtatt
     3061 tgctgaactg aatacgggct acggacggtg ctaaaaagat agttattcct agaactgaca
     3121 gttctaggaa taactcctat tttagctttg tccgcttgac gcccttaata tcttatatat
     3181 gcctaaacgt actgatattc aaaaaattat ggtgattggt tctggtccga ttattattgg
     3241 tcaggctgct gagtttgact acgctgggac tcaggcttgc ttgtcgttga aagaggaagg
     3301 ttatgaggtt gttttggtta actcaaatcc tgcaaccatc atgacggaca aggaaattgc
     3361 tgacaaggtt tacattgaac cgattacact tgagtttgtg acacgtattc ttcgtaagga
     3421 acgtccagat gccttgctac caacactcgg tggtcagaca ggtctcaaca tggccataga
     3481 attgtctaaa aatggtatct tagatgagct tggcgttgaa cttctgggta ccaaattatc
     3541 tgccattgac caagcggagg accgtgacct ctttaaacaa ttgatggaag agcttgaaca
     3601 acccattcca gaatctgaaa ttgttaacac agtagaagaa gcagttgcct ttgcagcgac
     3661 aattggctac ccagtcatcg ttcgtccagc ctttaccctc ggtggtactg gtggtggtat
     3721 gtgtgccaat gagaaagaat tgcgtgaaat cactgaaaat ggtttgaaat tgtcacctgt
     3781 tacccaatgt ttgattgagc gttcgattgc cggtttcaag gaaattgaat acgaagtgat
     3841 gcgtgactca gctgacaatg ccttggttgt ttgtaacatg gaaaactttg acccagttgg
     3901 gattcacaca ggggattcca ttgtatttgc ccctgcgcaa accatgtcag actatgaaaa
     3961 ccaaatgctt cgtgatgcga gcttgagcat tattcgtgcc ctcaagattg aaggtggatg
     4021 taatgttcag ctagctcttg atcctaacag cttcaagtat tatgttatcg aagtaaaccc
     4081 tcgtgtatca cgttcgtcag cccttgcttc taaagcgact ggatatccga ttgctaagtt
     4141 ggctgccaag attgcagtag gtttgacctt ggatgaggtt ataaacccag ttacaggttc
     4201 aacctatgcc atgtttgaac cagcccttga ctacgtagtt gctaagattc cacgtttccc
     4261 atttgacaag tttgaaaagg gtgaacgccg tctcggtaca caaatgaagg cgactggaga
     4321 agtcatggca atcggtcgta atatcgagga atctcttctc aaggcctgcc gctcccttga
     4381 aactggggtg caccacaatg aaatacctga acttgcagca gtttctgatg atgccttgat
     4441 tgaaaaggtt gtcaaagccc aagatgaccg tctcttctac gtatcagaag caattcgtcg
     4501 tggctacaca cctgaagaaa ttgcagagct aacaaaaatt gatatcttct atcttgataa
     4561 actcttgcat atctttgaaa ttgagcaaga attgggtgct catccacaag atctagaagt
     4621 tttgaaaaca gccaaactta atggtttctc agaccgtaag attgctgaac tctggggaac
     4681 gactgatgat caagttcgcc aacttcgctt ggaaaacaag attgttccag tttataagat
     4741 ggtagatact tgtgcggcag agttcgactc tgaaacgcca tatttctatt caacctatgg
     4801 tttggaaaat gaatctatca aatctgataa agaatctgta cttgtcctag gttcaggtcc
     4861 aatccgtatc ggtcaagggg ttgagtttga ctacgcaacc gttcactctg ttaaggctat
     4921 ccaggcagct ggttatgaag ctatcatcat gaactcaaac ccagagaccg tttcaacaga
     4981 cttctctgta tcagataagc tttactttga gccattgaca ttcgaagatg ttatgaacgt
     5041 cattgacttg gaacaaccaa agggagttat cgttcagttt ggtggtcaaa cagccatcaa
     5101 ccttgcggag ccattggcaa aagcaggtgt gaccatcctt ggtacacagg ttgctgacct
     5161 agaccgtgcc gaagaccgtg acctcttcga gcaagctctc aaagaattgg atattccaca
     5221 gccaccagga caaacggcta ccaatgaaga agaagcagct cttgcagctc gtaagattgg
     5281 cttcccagtt cttgtccgcc catcttatgt acttggtgga cgtgccatgg aaatcgttga
     5341 aaacgaagaa gaccttcgtt cttacatgcg aactgctgtt aaggctagtc cagatcatcc
     5401 agttcttgtc gactcttata tcgttgggca agagtgcgaa gttgatgcca tttcagatgg
     5461 taaagatgta ctcattcctg gtatcatgga gcatatcgaa cgtgctggtg tccactcagg
     5521 tgactcaatg gctgtttacc caccacaaac cttgtcgcaa aaagttcagg agaccatcgc
     5581 agactacacc aaacgcttag caatcggtct tcactgcctt ggaatgatga acatccagtt
     5641 tgttatcaag gatgaaaaag tctacgttat tgaggtcaat ccacgtgcca gccgtaccgt
     5701 tccatttcta tctaaggtaa ccaatattcc tatggctcaa gtagcgacca agctcattct
     5761 tggtcaaagt ttatcagaac ttggctacca aaatggactt taccctgaaa gcacccgtgt
     5821 tcatatcaag gcacctgtct tctcctttac taaactagct aaggtagaca gcttgctcgg
     5881 tcctgaaatg aagtcaacag gtgaagttat gggttctgat gctactttgg aaaaagctct
     5941 ctataaagcc tttgaagctt cttatctaca cttgccaact tttggtaatg tcgtctttac
     6001 catcgctgat gatgccaaag aagaagcctt gaacttggct cgtcgtttcc aaaacattgg
     6061 ttatggaatc cttgcgacag aagggacagc agccttcttt gctagtcatg gcttgcaagc
     6121 ccaacctgtt ggtaagattg gtgatgacga taaggatatc ccaagttttg tacgcaaagg
     6181 aagaattcaa gctatcatta acacagtagg aacaaaacga actgctgacg aagatggtga
     6241 gcaaattcgt cgttcagcca ttgaacacgg ggtgcccctc ttcacagcgc tagatacagc
     6301 taatgccatg cttaaggtac ttgaaagtcg tagctttgtt acagaagcga tttaaataga
     6361 aaaataagat atagcagact gaatttagaa cagtacacag gga
//
LOCUS       DAVKDZ010000098         6378 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000098 DAVKDZ010000000
VERSION     DAVKDZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.099"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            173..1456
                     /locus_tag="VWZ30_001923"
     CDS             173..1456
                     /locus_tag="VWZ30_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEU9335595.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILSYENKD"
     gene            complement(1651..3222)
                     /gene="ffh"
                     /locus_tag="VWZ30_001924"
     CDS             complement(1651..3222)
                     /gene="ffh"
                     /locus_tag="VWZ30_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU9335596.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHQVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPI
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSAPGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(3234..3566)
                     /locus_tag="VWZ30_001925"
     CDS             complement(3234..3566)
                     /locus_tag="VWZ30_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEU9335597.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            complement(3657..4034)
                     /locus_tag="VWZ30_001926"
     CDS             complement(3657..4034)
                     /locus_tag="VWZ30_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEU9335598.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGNYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            4106..5410
                     /locus_tag="VWZ30_001927"
     CDS             4106..5410
                     /locus_tag="VWZ30_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU9335599.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPVENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKNFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSLIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            5423..6229
                     /gene="yidA"
                     /locus_tag="VWZ30_001928"
     CDS             5423..6229
                     /gene="yidA"
                     /locus_tag="VWZ30_001928"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEU9335600.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
BASE COUNT         1791 a         1468 c         1195 g         1924 t
ORIGIN      
        1 ttttttctgc tatactatcc taagcaaagg tttttaatgt catcccgtga ggtgacgaag
       61 acgcagaaat atttaaaact ctttaaaatc taaattttaa agaagtctta ctctgagggc
      121 ctattgctgt aaaataatgg gctctttttt gatgcccaaa agtgaggttt atatgaaaca
      181 agaatcaact gttgatttgt tactagacgt tgaccaacgt ccttcggctg gaaaaggaat
      241 tctccttagt ttccaacacg ttttcgccat gtttggtgcg accatcttgg taccattgat
      301 tttgggaatg cctgtatctg ttgccctttt tgcttcaggt gttggaacac tcatttacat
      361 gattgctact ggttttaaag ttccagttta tctaggttct tcattcgcct ttatcacagc
      421 tatgtcactg gctatgaaag aaatgggggg ggatgtatct gctgctcaaa caggggttat
      481 cttgactggt ttggtctatg tccttgttgc taccagcatc cgatttgtag gaacaaaatg
      541 gattgataaa ctcttgccac caatcattat cggtcctatg atcatcgtta tcggtcttgg
      601 acttgcaggt tcagctgtta ccaatgcagg tcttgtagca gacggaaatt ggaaaaatgc
      661 tctggtagcc gttgttactt tcctaattgc tgcctttatc aatacaaaag gaaaaggctt
      721 cctacgaatc attccattcc tctttgccat tatcggtggt tacctcttcg cactaactct
      781 tggtttggtt gactttacgc ctgtcctaga agctaactgg tttgaaattc ctggtttcta
      841 cttgccattt agcacaggtg gtgcctttaa agagtacaat ctttactttg gtccagaagc
      901 catcgctatc ttgccaatcg ctatcgtaac aatttctgaa catatcggag accatactgt
      961 tttgggtcaa atctgtggcc gtcaattctt aaaagaacca ggtcttcacc gtactcttat
     1021 tggtgacggt atcgcaactt ctgtttctgc cttccttggt ggaccagcca atacaactta
     1081 cggagaaaat acaggggtta tcggtatgac tcgtatcgct tctgtctcag ttatccgtaa
     1141 cgctgccttc atcgcgattg ccctcagctt ccttggtaaa ttcactgcct tgatttcaac
     1201 tattccaaac gctgtacttg gtggtatgtc aatccttctc tatggggtta tcgccagcaa
     1261 tggtttgaaa gtcttgatta aagaacgtgt tgatttcgct caaatgcgaa acctcatcat
     1321 cgcaagtgct atgttggttc ttggacttgg aggagctatc cttaaacttg gtccagttac
     1381 actttcaggt actgcccttt cagccatgac aggaatcatc ttgaacttga tcttgtcata
     1441 cgaaaataaa gactaagagt ctaaatacac ctaatccact cagctgtctg agtggatttt
     1501 tcgtatacca taataaaagt gtcttaacaa aattattaaa atcaaaaaac gtataatatc
     1561 agatattcta aaaccttgat actgtacgtt ttatcataga aatttttact ttattttctc
     1621 atcaaatgag atttgcatca atctcttgtc ttacttgcgt ttcttcttcg ctttcttcat
     1681 tttgttagcc atacgtttca tggactgttt catggcaaat tcaccaattt tacctttcaa
     1741 accgccacca aacatctggc tcatatctgg cattcctgct cctccgggag ctgataagtc
     1801 aggcataccg ccttgtccca tcattccttc aagggcagac atatccattc ctcccatatt
     1861 tggcatattt ttaggaaggt tatttggatt aatccccatt tgcttcatca ttttattcat
     1921 atccccagac ataacaccct gcatgagctg tttagcctgg ttaaagtcct tgatgaattt
     1981 attgacttcg acgaatgtat ttccagaacc agcagcaata cgacggcgac ggcttggatt
     2041 taacaaatct gggttttcac gttcttcagg tgtcatcgaa gacacaatgg cacgtttacg
     2101 agcaatctgg cgttcatcca ccttcatgtt ttgaagtgct ggattattgg ccatacctgg
     2161 aatcatcttg agcaagtctt ccattggccc catgttttgt acctgatcca actgatcaat
     2221 gaaatcattg aaatcaaagg tattttcgcg catcttctca gccatttcaa gggctttttg
     2281 ttcatcgtat tcctgagaag ctttctcaat caaagtgagc atatccccca tgccaaggat
     2341 acggctagac atacggtctg ggtggaaggt ttcgatatct gtaatttttt cacctgtacc
     2401 agtgaacttg attggttttc cagtgatgtg acgaacagac agagcagcac caccacgagt
     2461 atcgccatca atcttggtaa ggatgacccc agtcacttcc aactgagcat taaactcacg
     2521 cgcaacattg gctgcttcct gaccaatcat agcatcaacg acaagcaaga tttcatttgg
     2581 ttgagccaat gctttcacat cacgaagctc attcatgagg agctcatcaa tctgcaaacg
     2641 acccgcagta tcaatcaaga catagtcgtt atgattagtt tgggcttgct ccaaaccttg
     2701 acgtacaatc tcaacagctg gtacttctgt tccaagtgca aagacaggca catcaatctg
     2761 ttgtcccaag gtcttaagct ggtcaatggc agctggacga taaatatccg ccgcaatcat
     2821 caaaggacga gcattttctt ctttcttgag tttgttggcc aatttaccag caaaggttgt
     2881 tttaccagcc ccttgtaaac caaccatcat gatgatgatt ggaatcttag gtgacttgat
     2941 aatttctgcc gtatcagaac ctaaaacggc tgtcagttcc tcatcaacga ttttaataat
     3001 ctgttgcgca ggattaagtg tatcaatgac ctgatgcccg actgcacgct cacgaacttt
     3061 cttgataaag tcctttacaa caggcaaggc aacgtcggcc tcgagcaagg ccaagcgaat
     3121 ttctttggtt gcctcttgga catcagattc agagattttt ccttttttac gtagattttt
     3181 aaagacgttc tgcaaacgtt ctgttaaact ttcaaatgcc atttttcttc ctcttattct
     3241 ctattatcaa tgcttgttaa aatttctatc tgctcctgca gaaagttatc cttgggatag
     3301 cgctccaaaa tctgatcaaa aatctgactg cggacaatat agtccgagta catgtgcaat
     3361 ttcatctcat aatcttccag aatcttttct gttcgcttga tattgtcata gacagcctga
     3421 cgactgacac cgaactcctc ggcaatttca gcaaggctgt aatcatcagc gtagtagagc
     3481 tcgatataat tcatttgctt atctgtcaaa agcgccgcat aaaattcaaa gagcgcattc
     3541 atacgattgg ttttttcgat ttccataact tttattatac caaaaattag cctaatctac
     3601 cacactagga agccgatcca agaagataga tagctaaatt tgaaaaagac atgagcctag
     3661 ccccaagtaa tttccaattg atagctggca aagggatgtc cctcttgatt ttgtagttga
     3721 taatctagtt caatcttttg cctatcaact tgataatggc tcgtttggat gataaactcc
     3781 tgcatgccca taggtgtagg aatataggct aaactatcgc tatcctttag aaagcgcata
     3841 atggtcttgg gattagaaaa tcggctcatc acaagttctt gaccatgaaa tttaatcact
     3901 actttttcct tttcctcatt ataaaagagt aaatagctat aatttccctt ttcatgcact
     3961 tccacatcat aaagctggtc aatcacttcc aactgctcat caaactgaat cgtatttcgc
     4021 atccgaatct tcacatcaag tcctctttct tgtctcttgt cctactattt taccaaaaag
     4081 agcaggattt tgctataatg gtcatatgaa cgaaaaagta ttccgtgacc cagttcacaa
     4141 ctacatccat gtcaataatc aaatcatcta tgacttgatt aatacaaaag aatttcagcg
     4201 tttgcgccgg atcaaacaac tgggaacttc cagttatacc ttccacggtg gagaacacag
     4261 tcgcttctct cactgtctag gagtctatga aattgcacga cgcatcacag agattttcga
     4321 agaaaaatat cctgaggaat ggaatcctgc cgagtctctc ttgaccatga ccgctgctct
     4381 cctacacgac cttgggcatg gtgcctactc ccatactttt gaaaatctct ttgatacaga
     4441 ccatgaagcc attactcagg agattattca aaatcctgag acagagattc accaagtcct
     4501 gctacaagtg gcacctgatt tcccagaaaa ggtggccagt gtcattgacc atacctatcc
     4561 taataagcag gtcgtgcagc tcatttctag tcagattgac gcagatcgca tggactatct
     4621 cttgcgcgac tcctatttta caggagcatc ctatggggaa tttgacctga ctcgcatcct
     4681 ccgagtcatt cgtcctgtcg aaaatggtat cgcctttcag cgcaatggca tgcacgccat
     4741 cgaagactac gtcctcagtc gctaccagat gtacatgcag gtttatttcc acccagcaac
     4801 acgcgccatg gaagttctcc tacagaatct tctcaagcgc gccaaggaac tctaccctga
     4861 ggacaagaat ttctttgcac gaacttcccc acatctcctg cctttcttcg aaaaaaatgt
     4921 gaccttgact gactatctgg ctctggatga tggcgtgatg aatacctact tccagctttg
     4981 gatgactagt cctgacaaaa ttcttgcaga tttatctcaa cgctttgtca accgcaaggt
     5041 ctttaaatcc attacctttt cacaagagga ccaagatcaa cttactagca tgagaaaatt
     5101 ggttgaggac atcggctttg atcccgacta ctatactgcc attcataaga actttgacct
     5161 cccttatgat atctatcgtc ccgaatctga aaacccacgg acacagattg agattttaca
     5221 aaaaaatgga gaactggccg aactctctag cctgtctctt atcgtccaat cccttgctgg
     5281 cagtcgccac ggagataatc gtttctattt ccctaaagaa atgttagacc aaaacagcat
     5341 cttcgcaagc attacccagc aatttttaca cttgattgag aacgatcatt ttaccccaaa
     5401 taaaaactag aagaggaaat ttatgagtat taaactaatt gccgtcgata ttgacggaac
     5461 cctagtcaac agccaaaagg aaatcactcc tgaagtcttt tctgccatcc aagatgccaa
     5521 agaagctggt gtcaaagtcg tgattgcaac tggccgcccc atcgcaggtg ttgccaaact
     5581 tctggacgac ttgcagttga gagaccaagg tgactatgtg gtgaccttca atggtgccct
     5641 tgtccaagaa actgctactg gccatgagat tatcagcgaa tccttgacct atgaggatta
     5701 tctggatatg gaattcctca gtcgcaagct cggtgtccac atgcatgcca ttaccaagga
     5761 cggtatctat actgcaaatc gcaatatcgg aaaatacact gtacacgaat caaccctcgt
     5821 cagcatgcct atcttctacc gtacccctga agaaatggct ggcaaagaaa ttgttaaatg
     5881 tatgtttatc gatgaaccag aaattctcga tgctgcgatt gaaaaaattc cagcagaatt
     5941 ttacgagcgc tactccatca acaaatctgc tcctttctac ctcgaactcc ttaaaaagaa
     6001 tgtagacaag ggttcagcca ttactcactt ggctgaaaaa ctcggattga ccaaagatga
     6061 aaccatggca atcggtgatg aagaaaatga ccgtgccatg ctggaagtcg ttggaaaccc
     6121 cgttgtcatg gaaaatggaa atccagaaat caaaaaaatc gccaaataca tcaccaaaac
     6181 aaatgacgaa tccggcgttg cccatgccat ccgaacatgg gtactgtaaa aggaaagaat
     6241 agataaaaac cgctcagctg agcggttttt gtatgaatat atcacaatga gagtaaatta
     6301 ataaatttaa ttgcagttgt acagttactt aaatcttgag agtacaaaaa ataggcattg
     6361 ctttcttaat ttgtggta
//
LOCUS       DAVKDZ010000099         6357 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000099 DAVKDZ010000000
VERSION     DAVKDZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.100"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            65..2023
                     /locus_tag="VWZ30_001929"
     CDS             65..2023
                     /locus_tag="VWZ30_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9335601.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTGRLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDILSVKSGGMLQNFGLEPGEVNEKAKAVGLDTYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAGAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            2195..2428
                     /locus_tag="VWZ30_001930"
     CDS             2195..2428
                     /locus_tag="VWZ30_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEU9335602.1"
                     /translation="MKLKLLRVDTKVIMGSFFLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKR"
     gene            2434..3876
                     /locus_tag="VWZ30_001931"
     CDS             2434..3876
                     /locus_tag="VWZ30_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9335603.1"
                     /translation="MRKKVIEGLIYAEKSFFDKNQSGELTSAIVNDTSVIREFLITTF
                     PNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMMFIILPLSNISEKYSRRLQEEIG
                     FLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRVTSFETPFALLFI
                     FATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTVYSRSKGSSVA
                     LENLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENRPVLKDITFSISKGQKIAFVGPS
                     GSGKSTIVRLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDN
                     LCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRLLSGGQSQRLQIARA
                     YLKDAEILIFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHRLSTVKDVDCIFVLE
                     EGKITGSGTHKELLENHERYARFVQEQMIE"
     gene            4034..5128
                     /locus_tag="VWZ30_001932"
     CDS             4034..5128
                     /locus_tag="VWZ30_001932"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9335604.1"
                     /translation="MSKELHLNTILAQAGIKSDDATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAVIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVVFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRYS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            5136..6302
                     /locus_tag="VWZ30_001933"
     CDS             5136..6302
                     /locus_tag="VWZ30_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9335605.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VELYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKC"
BASE COUNT         1957 a         1096 c         1393 g         1911 t
ORIGIN      
        1 taattgacta aacaaaccaa acgttgtata ataaaacaaa tatttaaagc aggaggttct
       61 ggaaatgaaa aagtctaaga gcaaatatct aaccttggca ggtcttgtcc tgggtacagg
      121 agttttattg agcgcgtgtg gaaattctag cacggcgtca aaaacataca actatgttta
      181 ttcaagtgat ccatctagct tgaactatct agcagaaaac cgcgcagcaa catccgatat
      241 tgttgcaaat ttggtagacg ggttattaga aaatgaccaa tatgggaata ttattccatc
      301 attagcagag gattggactg tttctcagga cggtttgacc tatacctaca aacttcgtaa
      361 ggatgccaag tggtttactt ctgagggaga agaatatgcg cctgtaactg cccaggattt
      421 tgtgacaggt ttgcaatatg cagctgataa aaaatcagaa gccttgtatc tagtgcagga
      481 ctctgttgct ggtttggatg actatatcac tggtaaaaca agcgactttt caactgtcgg
      541 tgtcaaggca cttgatgacc aaacggttca atatactttg gttaaaccag aactttactg
      601 gaattcaaaa acacttgcaa cgatactttt tcctgttaat gcagatttcc tgaaatcaaa
      661 aggggatgat tttgggaagg cggatccatc tagtattttg tacaatggac ctttcttgat
      721 gaaagcactt gtctcaaaat ctgctattga atataagaaa aaccctaatt actgggatgc
      781 taagaatgtc tttgtagacg atgtgaaatt gacctactat gatggtagcg accaagaatc
      841 actggaacgt aattttacag ctggtgctta tactacgggt cgtctttttc ctaacagctc
      901 cagctatgaa gggattaaag aaaaatacaa aaacaatatc atctatagta tgcaaaattc
      961 aacttcatat ttctttaatt ttaacctaga taggaagtct tacaattata cttctaaaac
     1021 aagtgacatt gaaaagaaat cgactcagga agcagttctc aataaaaact tccgtcaggc
     1081 tatcaatttt gcttttgaca gaacatctta tggggctcag tctgaaggga aagaaggtgc
     1141 aacaaagatt ttgcgtaacc tagtggttcc tccaaacttt gtcagtatca agggaaaaga
     1201 ctttggtgaa gttgtagcct ctaagatggt caactatggt aaggaatggc aaggtatcaa
     1261 ctttgcggat ggtcaagacc cttactacaa tcctgagaaa gccaaggcta agtttgcgga
     1321 agctaagaaa gaactcgaag caaagggtgt tcaattccca atccacttgg ataagactgt
     1381 ggaagtaaca gataaagtag gcatacaagg agttagttct atcaaacaat caattgaatc
     1441 tgttttaggt tctgataatg tagtgattga cattcagcaa ttaacatcag atgagtttga
     1501 cagttcaggc tattttgctc aaacagctgc tcagaaagac tatgatttat atcatggcgg
     1561 ttggggacct gattaccaag atccatcaac ttatctggac atcttgagtg tgaagagtgg
     1621 cggaatgctg caaaacttcg gtttagaacc aggtgaagtt aatgaaaaag ctaaggcggt
     1681 tgggcttgat acctatactc aaatgttgga agaagctaat aaagagcaag atccagccaa
     1741 acgttatgag aaatatgctg atattcaagc ttggttgatt gatagttctt tagttcttcc
     1801 aagtgtttcg cgtgggggaa ccccatcatt gagaagaacc gtaccatttg ctggtgccta
     1861 tggtttaacc ggtacaaaag gggttgaatc atataaatac ctcaaagtac aagataagat
     1921 tgtcacaaca gacgaatatg caaaagccag agaaaaatgg ttgaaagaaa aagaagaatc
     1981 caataaaaaa gcccaagaag aattggcaaa acatgtcaaa taaaatctaa ttgtagataa
     2041 gtttgtgtaa gaagtctttg tgcttaaaaa cgcataatat caggtatata aaatcctaga
     2101 tgttatgcgt tttgttatag gaagatttat tagattttat cctcaaattg agttattcct
     2161 gactttatcc gttgaaatat tataaggagt atttatgaaa ttaaaattat taagagtaga
     2221 tactaaggtg attatgggga gtttcttcct tgttctgtct agtctacttg ctttgttgct
     2281 tccccttatc ttaaagggtt taatagatgg gagttctatt gaaaatatag gttccaaagt
     2341 atttcaatcg tttttgattt ttattggtca agccttgttt tcttctattg gttactatct
     2401 ttttagtcaa tcgggtgaaa aaagatagca aaaatcagga aaaaagtgat agaggggttg
     2461 atttatgcag agaaatcctt ctttgataag aaccaaagtg gggagttgac ttctgccatt
     2521 gtcaatgata cgagtgtcat tcgtgagttt ttaattacga ctttcccaaa tattattctg
     2581 agtttagtta tggtacttgg ttccattgta gtcttattta gtcttgattg gaatctttct
     2641 ctacttttat tcatcactct tccttgtatg atgtttatta tcttgcccct ttccaatatc
     2701 agtgaaaagt atagtcgtcg tttacaggag gaaatcggat ttttaacagg gcaattgact
     2761 gaaaagattc aagaacatga attaattaag actaaccaag cagagaaaag tgtacaggat
     2821 gttttggata attgtattga aagggtacaa aacaattcac tgaaatcaga tagggttact
     2881 tcttttgaga ccccgtttgc tctcttattt atctttgcga ctatagctgt gatgctaacc
     2941 tatgggggtt atcgggtcag cgcaggatat atatctgtgg gaaccttggt ttcgtttttg
     3001 atttacctct ttcaattact taatcctatt agtaatatag ctaattttgt aactgtttat
     3061 tctaggagca agggatcttc agttgcactg gagaacttgc ttgcagttcc taaagaaaaa
     3121 tttgagggag gaaaatcggt atcaggacaa gggttgaatt ttaaccatgt ctattttggt
     3181 tatgatgaaa atcgacctgt cttaaaggat attacttttt caatttccaa ggggcaaaaa
     3241 attgcttttg ttggaccatc tggatcagga aaatcaacga ttgtgcgttt gctagagcgg
     3301 ttttataagc cgctttcagg agatattcta atggagcaat caagtatata tgattttaac
     3361 ttaaaagaat ggagaagtaa aatcgcttgg gtttcacaaa ataatgcagt cttatctggc
     3421 agtattcgtg acaatctttg tctcggtttg aatcgcttag taactgatga tgaattgatg
     3481 aaagtgctag acttagtatc actaggtgat gagattcgct ccatgaaaga gggactagat
     3541 actgaagttg gtgaacgcgg acgactcttg tcagggggac aaagccaaag acttcaaata
     3601 gctagagcct acttaaaaga tgctgaaatt cttatatttg atgaagccac tgctaatctt
     3661 gatgcggatt ctgagtatgc gattatcagt agcctctatt ctgtgctaaa ggagaagacg
     3721 gttgtgatta tagcgcatcg tttgtcaacg gtaaaagatg tggattgtat tttcgtctta
     3781 gaggagggga aaatcactgg ctcaggaact cataaggaat tactagaaaa tcatgagcgt
     3841 tatgctcgtt ttgtgcagga gcaaatgata gagtgaaggt gtcttttgag attcaccatt
     3901 ttatagtcta ttaaagggag caggaaaagc tcccttttta tatagtttga aactataact
     3961 agctcttgaa aacaagaaaa cgaaggatac aaaataagtg gtacaatagt tactatagat
     4021 ttggaggtat tgtatgagca aggaattaca ccttaacaca attttggccc aggcgggtat
     4081 taaatcagat gacgcgacag gtgcattggt gacaccgctt catttttcaa cgacctatca
     4141 gcatccagag tttggtcgat ctactgggtt tgattataca cgtactaaaa atccaactcg
     4201 cagtaaggct gaggaagtct tggcggttat tgagtcagca gactatgccc tagcgactag
     4261 ctcagggatg tcagctattg tactggcctt tagcgtcttt ccagtaggaa gtaaggtctt
     4321 ggcagtgcgt gatctttacg gtggttcttt tcgctggttt aaccaagtgg agcaggaagg
     4381 tcgtttccat tttacctatg ccaatacaga agaagagttg attgccgagt tagaaaagga
     4441 tgtggatgtt ctctatatcg aaaccccaac caatcccttg atgttggaat ttgatatcga
     4501 aaaactagca aaattggctc atgctaaggg tgccaaagtg gtggtggaca ataccttcta
     4561 tagccctatc taccaacgtc cgattgaaga tggagcagat atcgttctcc attcagcaac
     4621 caagtatcta gcaggccaca atgatgtctt ggctggagtg gttgtgacca atagtttaga
     4681 actatacgag aagctttttt acaatcttaa tacaacaggg gcagtcttgt ctccatttga
     4741 cagctaccag ttgcttcgtg gtctcaagac cttgtctctt cgtatggagc gctcaacagc
     4801 taacgcccaa gaagtggttg tctttttgaa ggattctcca gcagttaagg aagttctcta
     4861 cactggtcgt ggaggcatga tttcctttaa agtagccgat gaaacacgca ttcctcatat
     4921 tttgaacagt ctcaaggtct tctcttttgc ggaaagtttg ggtggagtgg aaagtcttat
     4981 tacttatcca acgactcaaa ctcatgctga tattccagca gaagtacgct attcttatgg
     5041 tttgacagat gacctcttgc gtttgtctat tgggattgag gatgctagag atttgattgc
     5101 agatttgcgc caagccttag aaggataaga caaagatggg aaaatatgat tttacaagcc
     5161 tgcccaaccg tttagggcac catacctata aatggaaaga aacagaaacg gatagtgaag
     5221 ttctaccagc ttggatagcg gatatggact ttgtggtctt gcctgaaatc cgccaagccg
     5281 tgcaaactta cgcagaccaa ctggtttatg gttataccta tgccagtgaa gacttaatta
     5341 aggaagttca aaagtgggaa gctacacaat acggttacaa ctttgacaaa gaggctcttg
     5401 tctttatcga gggtgtggta ccagccatct caacagctat tcaaaccttt acaaaagaag
     5461 gcgaggcggt tttaattaac acgcctgtct acccaccctt tgctcgcagt gtcaagttga
     5521 ataatcgtag attgattact aattccttag tggaaaagga tggtctgttt gagattgact
     5581 ttgaccaact tgaaaaggat ttggtggaag aggaggttga actctatatt ctttgcaacc
     5641 ctcacaatcc tggtggacgt gtttgggaaa aagaagtgtt ggagaagatt ggccaactct
     5701 gccaaaaaca cggtgttttg ttagtttcgg atgagattca ccaagatttg accctctttg
     5761 gtcacaaaca ccagtctttc aataccatca atcctgcctt caaaaatttt gctatcgtct
     5821 tgagcagtgc cactaaaaca tttaatattg ctggaacaaa aaattcctat gcagtcattg
     5881 aaaatcctaa gttgagactg gctttccaga aacgcctgtt ggccaataat cagcatgaaa
     5941 tttcaggctt gggttatttg gcgacagaag ctgcctatcg atacggtaaa gattggctag
     6001 aggaactcaa gcaagtcttt gaagaccaca tcaattatgt ggtggatcta tttggaaaag
     6061 agactaaaat caaggtcatg aaaccgcaag gtacctactt gatttggctt gacttttcag
     6121 cctatgacct gactgatgaa acattgcaag agttgttgag aaatgaagcc aaggttatcc
     6181 tcaaccgtgg tttggatttt ggagaggaag gaagtctcca tgcccgcatc aatatagcta
     6241 tgcccaaatc tctgttgcaa gaagtctgtc agcggattgt ggctactttt gccaaatgtt
     6301 aaaaatccag ccttctagga gaaaagtctt cctagaaggc tattttcata ggcgaaa
//
LOCUS       DAVKDZ010000100         6210 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000100 DAVKDZ010000000
VERSION     DAVKDZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.101"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            189..830
                     /locus_tag="VWZ30_001934"
     CDS             189..830
                     /locus_tag="VWZ30_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU9335606.1"
                     /translation="MLNKVKTKALISVGAVAATSFILMMGYTAGQHSTAKQSRKEIEL
                     AAAKLVEDKQAEDKASILSSDTVKEFLTQYYTKEKLGENNTRIQPYMTESAYSQELSS
                     QNDAMNQVYKDYILDYHFEKADIFVNQTTNQAIAMVSYNVTYVSDLKNANQSKTNQTE
                     TRTVKLSYSKLPGELLVNQVQVWKSGLDDLDKATPKTLEESSSISSLPNTTTK"
     gene            840..1925
                     /locus_tag="VWZ30_001935"
                     /pseudo
     CDS             840..1925
                     /locus_tag="VWZ30_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
     gene            1976..2209
                     /locus_tag="VWZ30_001936"
     CDS             1976..2209
                     /locus_tag="VWZ30_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335607.1"
                     /translation="MTIIERLEEKVTRQESKVARETEKLAAYKEQLETAMFATFKRRQ
                     SISHMSFEEALDHAFGRERQFDESEFRKDEMSE"
     gene            2206..2589
                     /locus_tag="VWZ30_001937"
     CDS             2206..2589
                     /locus_tag="VWZ30_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEU9335608.1"
                     /translation="MTKDWNFNQPLESKSENQEDPDKIAALFGNHQGGNDVNYEAAFQ
                     KRKQAPVTESNSSSKPKVTEVRTGKETDITTSYQQHLKRLIADNNSDIQSSQKKIEEL
                     HTLIDTKNKDNKKLQSIYDAISELH"
     gene            2627..2899
                     /locus_tag="VWZ30_001938"
     CDS             2627..2899
                     /locus_tag="VWZ30_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335609.1"
                     /translation="MFTTFFKKNQDNSDVFKKLIHRLSDMSVQDPEKIDRLLDIIFTP
                     DQGSEQLKTEATYREEILDDTLKEAKNQLHKEQLEKNLERFRKNSQ"
     gene            2969..3445
                     /locus_tag="VWZ30_001939"
     CDS             2969..3445
                     /locus_tag="VWZ30_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9335610.1"
                     /translation="MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMFNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            3445..4224
                     /locus_tag="VWZ30_001940"
     CDS             3445..4224
                     /locus_tag="VWZ30_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEU9335611.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLGYNLLIEGTLRTIDVPKETAQLLKSKGYEVQLALIATKPELSYLSTLIRYEE
                     LYAINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSGEDTT
                     SAATVLHDLLFGEWNTVEKEMLKIGQERLWVINKELNNRGD"
     gene            4226..5622
                     /locus_tag="VWZ30_001941"
                     /pseudo
     CDS             4226..5622
                     /locus_tag="VWZ30_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5624..>6210
                     /locus_tag="VWZ30_001942"
     CDS             5624..>6210
                     /locus_tag="VWZ30_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335612.1"
                     /translation="MRDIVIKFLQNNHFNQLDTDFDFYTNSQKSEYFIVSQYSPDELN
                     NFFDDDKTSQVIKEFERVSLTPEHENIKKNTSMFILVEVDDLKEVFEDEKVQRSILLI
                     EEDYYYFRKFIILYTRNGLLDLRDKETNEILYTYLESNIDAFEDDMFFSESYFMAMEI
                     GVKLPFFTLPKRNDIYQSIESQYQDDKDELDNRLL"
BASE COUNT         2307 a          845 c         1210 g         1848 t
ORIGIN      
        1 tgtagaacaa ggaagtcata cagaactatt gaatatgaaa aatggtttgt ataaaaaact
       61 ttatcagacg gattactaat tttgtaagtt atattggcca ccttcgggtg gcatttttac
      121 ttctgtgata cttttttcaa gatttatctg ctattcttga cctagttttt atagaaagaa
      181 ggtctaagat gttaaataaa gtcaaaacta aagccttaat tagtgttgga gcagtggctg
      241 caactagctt tattctcatg atgggatata ctgctggtca acactcgact gctaaacaaa
      301 gtcgtaaaga gattgaattg gctgcagcta aacttgtaga ggataaacaa gcagaagata
      361 aagccagcat tttgtcatcg gatactgtaa aagaattttt gacgcagtac tatacgaaag
      421 aaaagctcgg agaaaataat acacgtattc aaccttatat gactgaatcg gcttattctc
      481 aagaattgtc aagtcaaaat gatgccatga accaagtgta taaggattat attttagatt
      541 atcattttga aaaagctgat atctttgtca atcagactac gaatcaagcc attgccatgg
      601 tctcttataa tgtaacctat gtatctgatt taaagaatgc caaccaatca aagaccaatc
      661 agacagaaac cagaacggtt aagttgtctt attcgaaact accaggtgag ttattggtca
      721 atcaagtaca ggtttggaaa tctgggttag atgatttgga taaggccact cctaaaactt
      781 tagaagaatc atcatcaata tcatcactgc caaatactac gacaaaatga tgatattgca
      841 tggaaataga agaatgtaag caaatttcaa ttcttgatgt agccaatcgt ttaggtatct
      901 cctttaaaca agtttctagc cgtgtctatg aacatcctga acacgattca tttcggattt
      961 tttcaactac caatactttt aaatggtttt caagagatat tcaaggtgat gtcattgatt
     1021 ttgttcgact tgttaaggga atttccttta aagaagctct agcctttctt tctgaagaac
     1081 cttttcaaaa agaagctgtt caagaaaaaa gagagagacc attttattat cctttaaaga
     1141 gagtagaaga ttctaactgc agtctggcta gatattattt aacagaatgt agaggaattt
     1201 cagaagaaat cgttcaaaag atgattcaac aaggtttgat agcacaagct agctggaaaa
     1261 caaatgaaac tgttgaacct gttattgttt ttaaaagctt tgatcatcgt tacaagctgc
     1321 aggcagcaag tctctaaggg atttataaga atcactctct tcctagagag aggttaaaaa
     1381 cgattctaaa aggaagccat ggacatgttg gaatatcctt tgatattggt aaacctaata
     1441 gactggtctt ttgtgaatcg ttcatcgact taatgagcta ttacgaactt catcaacaaa
     1501 gtctatctga tgttcgtttg gtatctatgg aaggattaaa aaggtctgtt gttgcttatc
     1561 aaattttacg actgatagct gaagaaaatc agaagttgga atttttggat acagtaatac
     1621 cttcaaagtt attgccttta atcaatacaa ttcgtgatac caccagctat tttgataatc
     1681 atcctgattt attgacactt gcggtagatt gtgatgatgc aggaaaagat ttttctgata
     1741 agttatctca atcaggattt cctgttttcc tggatttccc tgataataaa tctgggaaag
     1801 aaaaagtaga ctggaatgat gtccttagag aaaagaaatc agatttacaa ttgatgatcg
     1861 agaatgcaaa agagacattg aggaatcaac cagtaagaaa aacctctcaa tgtttagaat
     1921 tgtgataaca aaatcaagat gttttagcta atattagaat gaaggaggat cgtatatgac
     1981 cattattgaa cgcttagaag aaaaggtcac taggcaagaa agtaaggtag caagagagac
     2041 agaaaaactc gctgcttaca aagaacaact ggagacagcg atgtttgcga cgttcaaaag
     2101 gcgtcaaagc attagtcaca tgagttttga agaagctctt gaccatgcct tcggtagaga
     2161 aagacaattc gatgaatctg aatttagaaa ggatgaaatg agtgaatgac aaaagattgg
     2221 aattttaatc aaccattaga aagtaaatca gaaaatcaag aagatccaga taaaattgcg
     2281 gccttatttg gaaatcatca aggaggtaat gatgtaaatt atgaagcggc ttttcaaaag
     2341 cgcaaacaag cacctgtgac ggaatcaaat tctagttcta aacccaaagt tacagaggtt
     2401 agaacaggaa aagagacaga tatcactaca agttatcagc aacatcttaa aagactgatt
     2461 gcggataaca atagtgatat tcaaagtagt caaaagaaaa ttgaagagct acatacgttg
     2521 attgacacaa agaataaaga caataagaaa ttgcagtcca tttatgatgc gatttcggaa
     2581 ttacactaag cagtcctgat aggctgcttt ttttgagagg ttatagatgt ttacaacatt
     2641 ttttaagaaa aaccaagaca atagtgatgt atttaagaag ctaattcata gactatctga
     2701 tatgtctgtc caagatcctg aaaaaataga ccgactgtta gatatcattt tcactccaga
     2761 tcaaggatca gaacaactaa aaacagaagc aacttacaga gaagaaattt tggacgacac
     2821 attaaaagaa gctaagaatc aacttcataa ggaacaattg gagaagaatt tagagagatt
     2881 taggaaaaac agtcaataac gcaccaaaaa tggtgcattt gctttttcta aatgttataa
     2941 tggtggtatc aaataaaagg agtttgctat gattggaaag aacataaaat ccttacgtaa
     3001 aacacatgac ttaacacaac ccgaatttgc acgaattata ggaatttctc gaaatagctt
     3061 gagtcgttat gaaaatggaa ctagttcagt ctctacggaa ctaatagata tcatttgtca
     3121 gaagtttaat gtatcttatg tcgatattgt aggagaagat aaaatgttca atcctgttga
     3181 agattatgaa ttgactttga aaattgaaat tgtgaaagaa agaggtgcta atctattatc
     3241 tcgactctat cgttatcaag atagtcaggg aattagcatt gatgatgaat ctaatccttg
     3301 gattttaatg agtgatgatc tatctgactt gattcatacg aatatctatt tagtagaaac
     3361 ttttgatgaa atagagagat atagtggcta tttggatgga attgaacgta tgttagagat
     3421 atctgaaaag cggatggtag cttaatggaa atccaagatt atactgatag tgaattcaaa
     3481 catgctttag caaggaatct tcgttcactg acaagaggaa aaaagtccag taagcaacct
     3541 atagcgattt tgcttggagg gcaaagtggt gctggtaaga ctacaattca tcgtattaaa
     3601 cagaaagaat ttcaaggaaa tattgttatc atagatggtg atagttttcg ttctcagcat
     3661 ccacactatt tagaactgca gcaagaatat ggcaaagata gcgttgaata caccaaagat
     3721 tttgcaggaa aaatggtaga gtctttagta acaaaattga gtagtttggg atacaatctt
     3781 ttgatcgagg gaactttacg aacgattgac gttccaaagg aaacagcaca actcttgaaa
     3841 agtaagggat atgaagtaca attagccttg attgcgacaa agcctgagct gtcctatctg
     3901 agcactctta tccgatacga agaactgtac gctatcaatc caaatcaagc acgcgcaact
     3961 ccaaaagaac atcatgattt cattgtaaat catctagttg ataatacacg gcaattagaa
     4021 gaactagcta tctttgaaag aattcaaatt taccaacgag atagaagttg tgtatatgat
     4081 tcaggagaag atacaacttc agcagcaaca gttcttcatg acttactatt tggagaatgg
     4141 aacacggttg aaaaagagat gttgaagata gggcaggaac ggttgtgggt gattaacaaa
     4201 gagttaaata atagggggga ttaagttggc gagttcaaac gttgaccaaa ataggaatgc
     4261 aacaccaagt tggagtggat atatccatca agggaaagta ggatttttag tagcgttaag
     4321 acaattgaaa aaatgtattg atgataaggc gcctgattac gagaattatg tgattagata
     4381 tgaaaatgct gaagattttg atattgttga taacaacgac aaagttatat ctaggcatca
     4441 agtaaaggca tatatagatg gtacgtcaag ggaacaatat ttaagataca aacaagaaaa
     4501 tattgtgagg ttgaggataa gagaagtaat caaaagattt ggaaagaaat aattgatgag
     4561 gaaggatttc aaatccatca atttgatggg caaggacaca tactaactgt agaagttagt
     4621 cctgattcaa gatacttaca cgttattgta gcaataacag atttcaattt atctcggtat
     4681 gagtatttaa agaaagacgg tcgcagaaaa aaatatactg ataataattc atgtattcaa
     4741 ctatatccat acaataaaga gaacgatctt tatcattgtc cactttctca ggatgataat
     4801 aatgatacaa ttagagatta ttgtaaagct gaaataaaag aaattttaaa atcaaagggt
     4861 aattcattga atgatagtgt ctcacattta gaccaagtat atctcaggta tgtaggttct
     4921 ttattggatc atagcattgg acaagaacac agtgagtctg gttttccaga aatatccttt
     4981 ggagaaatac tttccattat tgaaacggaa acttctgaag atgaagtata tgaaatgaaa
     5041 aaaaatttga cctataggtg ggaaaaatat catggtgatt atgcaagtga cattagagag
     5101 gaagatttta aacttatgga tgaaataaat aatcatttac tgtcattgac taaagatgag
     5161 ttttatgaat ttgtgagaat gatgttacca cacgaaaatt cagatgaaaa atttttagaa
     5221 ttgtttcgta atgccccaat ggaggatata ttttatgtat tactggagaa aattaaggga
     5281 ttttcgttta aatcttacag ttatttagat gataatgaga agagctatag aacttcattg
     5341 attagtattc aggatagacc tgggataatt gcaaaaaaaa ttcaagaaat aattgacaac
     5401 tcagaatttt taaaagctac ttttaatcat gatttcctta ttaatagaga tatagatgag
     5461 ttgcatattg ggaatagaat tgatgaattt gatggcatgc tagataatag atatcgtgaa
     5521 gaatggaata caggagtaca acataatata tttaaaccga atatggaatt cattagcatt
     5581 gagaaagtaa aagaaaagaa tttgagtgtg ccggaggact aatatgagag acatcgttat
     5641 aaaattttta caaaataacc attttaacca gttggataca gattttgatt tctatactaa
     5701 ctcacaaaaa tcagagtatt ttattgttag tcaatatagt cctgatgagc taaataattt
     5761 tttcgatgat gataagactt cacaagtaat aaaagagttt gaaagggtat ctctaacacc
     5821 agaacatgaa aacatcaaga aaaatacttc tatgtttatt ttggtagaag ttgatgactt
     5881 gaaggaagta tttgaagacg agaaagttca acgatcaatt ttattgattg aagaggacta
     5941 ttattatttt agaaagttca taatattata cactcgaaat gggttattgg atttacgaga
     6001 taaagagact aatgaaatac tctatactta cttagaatct aatattgatg cttttgaaga
     6061 tgatatgttt tttagtgagt cgtattttat ggctatggaa attggagtaa aactaccatt
     6121 tttcacactc cctaaaagaa atgatattta tcaaagtata gaaagtcagt atcaggatga
     6181 taaggatgaa ttagataata gattattgga
//
LOCUS       DAVKDZ010000101         6131 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000101 DAVKDZ010000000
VERSION     DAVKDZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.102"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            238..>6131
                     /locus_tag="VWZ30_001943"
     CDS             238..>6131
                     /locus_tag="VWZ30_001943"
                     /inference="COORDINATES: protein motif:HMM:NF020018.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473604.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9335613.1"
                     /translation="MKKSYRDDNGEKVFRYSIRKYHFGAASVAVAALMFFANGAVAAS
                     ETITPTTASDVVKAGSDGNADGNPASSDEGDSKQALTDKPAELKAADELKTQEAPAEG
                     ANQTSSTGILQPRTLKNNQSDYDPIPIEDDEDDVRDGLFSSENPVTTIQPRSSHSVTG
                     RALQGDTQAPKVSLGSALLPTSESTATTPLYRIRQGQAFTPKLKVWNNSGSIKSLDIT
                     GIPNGVTKHRFGTDFSEQTAATEMNPYSGSTLSGNVPDTQAVGQHIAQITVKDASNNV
                     ATYYLKYEVYPARVEAKQGRFGQVKDKALIHGDDPANYIKFKSANGKEVQKPADVKVT
                     WERKPSTVEAGLNKTGLVKVTYNVTDENGVVRDEVQTVTINTPVYHATLVQNPFKTTY
                     GREFVNKNQPRDGRRYIDYNGRSHFNLSNLRVYWESSNIRGQQFSDGTRPWPTNYLGK
                     KREKLMVRYPGDNGRYDNRNDDYGARYEELDGTFIVKPVKPNIQTSLGKVGKNTLTVN
                     NVNSGTTVVVYDAANPNNLKELGRTMVAKEGDYRIKNGVEVPLNSGVTFKKDQKIVTK
                     VIYKITDDDQRTESDVSDIWTVKESLIANGIHVIKGESYTGTAKDRIRYNDNVDAEQR
                     TGLPNNATASWAQNPNYTTLGTSNYTANVTVPGQGSATVNVPVHVYAPANLKATSYNN
                     KQGTLSNGANPENYIEFKDGNTVVTKPNNVTVRWQGGVAPRINTPGHNRGVIEVVYPG
                     NAGASSTVVKTFTVQLPTYHTTATATEYTRTIGENFAKTTARDYATTHNWNVGGSQYV
                     WKNDETANREYSAENWGKVNGDWLGKKKNKVKVYYGNDNGSNSHSENLAEETEEITFV
                     TKPKTPSITATALNGKAGQRNQQVTVNNVTPGTTVELYNGDTKIGSVDVSKANNEAYT
                     DIKTVTVAVNGQLPLSSNIRAKTIYMPNNANEKVESDFSTSVQSTTEGPQAPEISQNP
                     ENLLVKATVGQGGSTKVTLTYTDANGRVKEVGFTKNGQFWDKDDANADTTVSITNESN
                     GTGEIQLQPGTAQEGTTVTVKQKTATSEFSTPATTKALGRLNGLTNVAQADGSVDITV
                     PDTATKFDLTYRNQQNNTTETLHYSKDSQGRWGNVTGITADGNRFTLPKGLVTDGTTV
                     SVIASNDNKTTKTVTSDAKFEMPDATTHTQRENGDGVITLPSTADSVTVTYTDAQDTA
                     KTVTVTKNASNQWASTAALPEGVTLNGNELNVAYKNINSNGNVKTVSTRGTGNVRSQE
                     AVENIELNHIPVSTQAVVIAAGATPTNDDLGRGVTFAKRSITAKSTPAAVPAGTSAEI
                     PATLTYNDGSTEDVTITVKSKPTAPSFNNLENHGTYSGLSSISKVISGTAMPGAEKVK
                     LTLQDGTVKEITPQADGSWSYTLGATEYLTQSFSGQFNGVFNQNKVKAVQVKDGVESE
                     ETTANVAPGQATVDSVYKAGRAITVNIPHDIEAGYVRVNGTDYGIQKVNGTWRVISTA
                     PNAAKLEISNTVVDPTNKAVTKVTFSVKNNDDALYTPPFKIGNSPVRFRTYYSKNGTI
                     NTPTPQIRGTDGWADSPTPKNTKPTVNFTADHTIEENKVFASPTVEELKDYFEGHDAE
                     DDASLTVGYSASNRGKLRVQVFTQDTNQSVRANTQGRIDPGNYRLVLSTNDAADAESE
                     PITRNITVKTYADVYRDKVLYPTNVDKVTYDDTAISNGNFTTAAKTSFKDKIQELNGT
                     VLPASTRYTVGTTDDKAKVAVINFPDGSTIDISHAVVAKPTVPTVTRTHADKVSDADR
                     TISGTALQSATKVTIYFQDGRGEQGHADVVPVNGQWTYTLPTGRYLRQTEHTSLPGSS
                     SVPVNVTQTVFDATSDKTAVYVAKDRNFTGKQIVGTKGSQELTALKNDAKKGINYTER
                     NTPKDFPSDFDATWKETPDITTVGTRTYTAKVFEKDKGDAVSQEISVEVTVKADTPDA
                     VAASYK"
BASE COUNT         2087 a         1198 c         1345 g         1501 t
ORIGIN      
        1 gactaggctg gttcggtatc tttctgaaaa aatccttaac aaacgccagc aaaaagttag
       61 cgtttttctg ttcaaatttg cgaaaatagt atataatagt actgtctcaa aacttgtctt
      121 ttgacaagcc tgggaacaaa aagaaaaaaa catatccttt ccttaaatac taaaatattt
      181 aggggggggg gtatttttta gtacctccag aaaggaaaaa cgagaggtaa ttcaatcatg
      241 aaaaaatcat atcgtgatga taacggggaa aaggtcttcc gttattccat tcgtaagtac
      301 cactttggtg ctgccagtgt tgcggtagcg gctctgatgt tttttgccaa cggagctgtg
      361 gcagcgagtg aaacgattac tccaactacg gcaagtgatg tcgttaaggc aggttctgat
      421 ggcaatgcag atggaaatcc agcgtcctcg gatgagggag actccaagca agctctgact
      481 gacaaaccag ctgaactcaa agcagctgat gagctgaaaa cgcaagaagc gccagctgag
      541 ggagccaatc aaacaagtag tactggaatc ctccagccgc ggacgctcaa aaacaatcag
      601 tctgactacg accctattcc aattgaggat gatgaggatg acgtaagaga tgggctgttt
      661 tcttcggaaa atccagtaac cactatccaa ccacggagct ctcactctgt aaccggtcgc
      721 gcacttcaag gcgacacaca agcgccaaaa gtgagtttgg gcagtgctct tcttccgact
      781 agcgaaagta cggctactac gccattgtac agaatccggc aagggcaagc ttttacaccg
      841 aaattaaaag tttggaataa ctcgggatct atcaaaagct tagatataac gggaataccg
      901 aatggagtga ccaagcatag atttggaact gacttttcag aacagacggc agctactgaa
      961 atgaatccct acagtgggtc aacacttagt ggtaatgtgc cagatacaca agcagtagga
     1021 caacatatag ctcaaattac tgtaaaagat gcatcgaata atgtagctac ttactaccta
     1081 aaatatgaag tctatccagc aagagtagaa gctaagcaag gtcgatttgg acaagtcaaa
     1141 gataaagcct tgatacatgg cgatgatcca gctaactata tcaagtttaa aagcgctaat
     1201 ggaaaagagg ttcaaaaacc agcagatgta aaggtgacgt gggaacgtaa gccaagtaca
     1261 gtagaagcag gtcttaataa aacaggacta gtaaaggtta cctacaatgt aacagatgaa
     1321 aatggtgtgg ttcgagatga agttcaaact gtaacaatca atacccctgt ctatcatgca
     1381 actcttgtac agaatccctt taaaacaacc tatggtaggg aatttgtcaa taaaaatcaa
     1441 cctagagatg gtagacgcta cattgactat aatggaagat ctcatttcaa cttaagcaat
     1501 ctacgtgttt actgggagag ttctaatata agaggtcagc aattttctga tggtacgcga
     1561 ccatggccta ccaattattt aggtaagaaa cgtgagaaat tgatggttcg ctatccaggt
     1621 gataatggca ggtatgataa tcgtaatgat gactacggtg cacgatatga agaactagat
     1681 ggaactttca tcgttaagcc agtcaaacca aacattcaaa ccagtcttgg taaggtagga
     1741 aaaaatactc tgactgtcaa taatgtcaat agtggaacaa cagttgtggt ctatgatgct
     1801 gccaatccaa acaatctcaa ggaattgggt cgtacaatgg ttgctaaaga aggggattac
     1861 aggattaaaa acggagttga agtgccacta aactctggtg taactttcaa aaaagaccag
     1921 aaaattgtca ccaaggtcat ctataagatt acagatgatg accaacgtac agaatctgat
     1981 gtcagtgata tctggacggt caaggaaagc ttgatagcaa atggcatcca tgtcatcaag
     2041 ggcgagagct atacaggaac tgccaaagac cgtatccgtt acaacgataa tgtagatgcg
     2101 gaacagcgaa caggccttcc aaacaatgcc acagcaagct gggcgcaaaa tccaaattat
     2161 actactttgg gaacaagcaa ctacactgcc aatgtaacag tgccagggca aggaagcgcg
     2221 acagttaacg tgcctgtgca tgtctatgca ccagctaacc tgaaggcaac ttcttacaat
     2281 aacaagcaag gtaccctatc aaatggtgcc aatccagaaa actatattga atttaaagat
     2341 ggaaatacag ttgtcactaa accaaataat gtgactgtgc gttggcaagg tggtgtagca
     2401 cctcgaatca atacaccagg tcacaataga ggagttattg aggttgtcta tccaggaaat
     2461 gctggagcaa gctctacggt tgttaagacc tttacggttc aattaccaac ctaccataca
     2521 acagctactg ctacagagta cactcgtacc attggcgaaa actttgccaa aacaactgct
     2581 cgcgactatg cgactacgca taattggaat gtaggtggca gtcagtacgt atggaaaaat
     2641 gatgaaaccg ctaatcgtga atacagtgcg gaaaactggg gcaaggtcaa tggcgattgg
     2701 cttggtaaga agaaaaataa ggttaaggtt tactacggaa atgataacgg aagcaactcg
     2761 cattctgaaa atctagccga agagactgaa gaaatcacct ttgtgactaa gccaaaaaca
     2821 ccaagcatca ctgcgacagc tcttaatgga aaagcgggtc aacgcaatca acaagtgaca
     2881 gtaaacaatg tcacaccagg gacaacagtt gagctctaca acggtgatac gaaaattggt
     2941 tctgtagatg tatcaaaagc taataacgaa gcttacactg acattaaaac tgttacagta
     3001 gcagttaatg gtcagttacc actttcatct aatattcgtg caaaaacgat ttatatgcct
     3061 aataatgcaa atgaaaaagt agaatctgat tttagtacta gtgtgcaaag tacaacagaa
     3121 ggtccacaag cgccggaaat ttctcaaaat ccagaaaact tattggtgaa agcgactgta
     3181 ggacaaggag gttctactaa ggttactctt acttatacag atgctaatgg acgtgtgaaa
     3241 gaggttggat tcacgaaaaa tggtcaattc tgggataagg atgatgccaa cgctgatact
     3301 actgtatcga ttactaatga gtctaatgga acaggtgaaa ttcaacttca accaggtacg
     3361 gctcaagagg gaacaacagt aacagttaag caaaaaacag ctacatcaga atttagtact
     3421 ccagccacta ctaaagcttt aggacgctta aatggcttga cgaatgtagc tcaagcagat
     3481 ggttcggtag atattactgt accagatacg gctacgaagt ttgatttgac ctaccgcaac
     3541 cagcaaaata atacaactga aacacttcac tatagcaaag actcccaagg acgttgggga
     3601 aatgtgacag gcattactgc tgacggcaat cgctttacct tgccaaaagg tttggtgact
     3661 gacgggacaa ccgtttctgt catcgcatca aatgacaata agacaactaa gacggttacg
     3721 agcgatgcta agtttgaaat gccggatgct acaactcata ctcagcgtga gaatggtgac
     3781 ggtgttatta cgcttccaag tactgctgac tctgtgacgg tcacttatac ggacgctcaa
     3841 gatactgcta agacggtcac tgtgactaag aatgctagca atcaatgggc aagtactgct
     3901 gccttgccag aaggtgtgac tttgaacggt aacgagctca atgttgctta taagaatatc
     3961 aatagtaacg gaaatgtgaa aacagtttcg acacgtggta ctggcaatgt tcgttctcaa
     4021 gaggctgtcg aaaatattga attgaaccat ataccggtaa gcactcaagc ggttgttatc
     4081 gctgctggtg cgacaccgac aaatgatgat ttaggacgtg gtgtgacatt tgccaaacgt
     4141 tctatcactg ctaagtcaac accggcagct gttccagcag gaaccagtgc agaaattcca
     4201 gctaccttga cttataatga cggctctact gaggatgtca ctattactgt taagtctaaa
     4261 ccaacagcac caagcttcaa taatcttgaa aatcatggaa catatagcgg attatcctct
     4321 atttctaagg tgatttcagg tacagctatg ccaggtgcag aaaaagttaa attgacttta
     4381 caagacggta cagttaaaga gataactcct caagctgatg gaagttggtc ttatacttta
     4441 ggagctactg aatacttgac gcaaagcttc agtggtcagt ttaacggagt attcaatcaa
     4501 aataaggtaa aagctgtcca agtgaaagac ggtgtagagt cagaggagac tacagcaaac
     4561 gttgcaccag gtcaagcgac tgttgattct gtttataagg caggacgtgc gattacggtc
     4621 aatatccctc atgatattga agctggttat gttcgtgtca acggaacaga ctacggtatt
     4681 caaaaagtta atggtacttg gagagtaatc tcaacagcac caaatgctgc caaattggag
     4741 atttcaaata cagttgttga tccaacgaat aaagctgtca ctaaagtgac ctttagtgtt
     4801 aagaataatg acgatgcctt gtatactcca ccatttaaaa ttgggaatag tcctgtaaga
     4861 ttcagaacgt attattctaa aaacggaacc attaacactc caacacctca aattcgaggg
     4921 acagatggat gggctgattc tcctacacct aagaacacta aaccaactgt aaacttcaca
     4981 gctgatcata caatagaaga aaacaaggtg ttcgcttctc caactgtaga agagttgaag
     5041 gattattttg aaggtcatga tgctgaagat gatgcaagtt taactgtagg ttactctgct
     5101 tctaatagag gtaaattacg tgttcaagta ttcactcaag acactaatca atcagtaagg
     5161 gctaatactc aaggacgtat tgatccaggt aattacagac ttgtcttgtc tacgaatgac
     5221 gcggcagatg cagagtctga accgattaca agaaatatca cagtgaaaac ttatgctgat
     5281 gtttatcgtg ataaggttct ttatccaacg aatgttgata aggtgactta tgatgataca
     5341 gcgattagta atggtaactt tacaacagca gccaaaacaa gctttaaaga taaaattcaa
     5401 gagctaaatg gcacggtgct tccagcatcg acaagatata cagtaggaac tacggacgat
     5461 aaggcgaaag tcgctgtgat taacttcccg gatggttcta ctattgatat ttcgcatgcg
     5521 gttgtagcca aaccaacagt gccaacggta actcgcacac atgctgataa agtatcagat
     5581 gctgacagaa caatttctgg tacagccctt caaagcgcga caaaagtaac tatttatttc
     5641 caagatgggc gtggtgaaca aggacatgca gatgttgtgc ctgtaaatgg tcaatggact
     5701 tacactcttc caacaggacg ttatctccgc caaacagaac acacttcatt accgggttct
     5761 tcctctgtac ctgtaaacgt aacacaaacg gtatttgatg caacaagcga taaaacagct
     5821 gtttatgtgg cgaaagaccg taactttaca ggaaaacaaa tcgttggaac taagggtagt
     5881 caagaattga ctgctttgaa aaatgatgct aaaaaaggaa tcaactatac tgaaagaaat
     5941 actccaaaag actttccaag tgattttgat gctacatgga aagaaacacc agatattaca
     6001 actgtaggaa caagaactta cacggctaag gtgtttgaaa aagataaggg tgatgcagtt
     6061 agccaagaaa tttcagttga agtcactgtc aaagcagata ctccagatgc agtagcagca
     6121 agctacaaac a
//
LOCUS       DAVKDZ010000102         6093 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000102 DAVKDZ010000000
VERSION     DAVKDZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.103"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(661..1545)
                     /locus_tag="VWZ30_001944"
     CDS             complement(661..1545)
                     /locus_tag="VWZ30_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU9335614.1"
                     /translation="MMEKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKQIKECL
                     HQMILDQEQIQEIILSLWENSVVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(1549..1935)
                     /locus_tag="VWZ30_001945"
     CDS             complement(1549..1935)
                     /locus_tag="VWZ30_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEU9335615.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEV"
     gene            complement(1928..3271)
                     /locus_tag="VWZ30_001946"
     CDS             complement(1928..3271)
                     /locus_tag="VWZ30_001946"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU9335616.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLDLGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNIKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWSNSGNYDASVKQALADMLEVFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(3353..4237)
                     /locus_tag="VWZ30_001947"
     CDS             complement(3353..4237)
                     /locus_tag="VWZ30_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9335617.1"
                     /translation="MIQVRNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRLYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPKRGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(4249..4866)
                     /gene="cysE"
                     /locus_tag="VWZ30_001948"
     CDS             complement(4249..4866)
                     /gene="cysE"
                     /locus_tag="VWZ30_001948"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU9335618.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(4882..>6093)
                     /gene="pnp"
                     /locus_tag="VWZ30_001949"
     CDS             complement(4882..>6093)
                     /gene="pnp"
                     /locus_tag="VWZ30_001949"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU9335619.1"
                     /translation="DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKR
                     FMHHYNFPQYSVGETGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLE
                     SNGSSSQASICAGTLALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDM
                     DFKVAGTRDGITALQMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELA
                     PTAPKIDTIKIDVDKIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINR
                     AKEIIAGLVREAKVDEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDL
                     VEIGDEVDVKVIKIDEKGRIDASMKALLPRSPKPEHDEKGEKSERPHRPRHQKDHKPK
                     KEFTETPKDSE"
BASE COUNT         1750 a         1444 c         1142 g         1757 t
ORIGIN      
        1 atccttctcc tccttctatt ataccatagt tttaaatcta attttgctag attctatggt
       61 tctatctatt tattcgaaaa agaaaggaga aagttaaaaa tttttagact tcacaggttg
      121 aaatttacat aaaaaagcag ctgattcttt ttttgattct accatatttc tagcgattac
      181 tcctataaat aggatacatc acagtttcta gtaaaaaagg acgctgaaaa gcaaacaaaa
      241 cccagtatta ggaagagtta aatcttatct gaaaatgact agctctcaag ctatagtttt
      301 gtgtctaaat ataagtatca ctaggaaaaa ctccgtaagt aaacacaccg aaattggatg
      361 ttcctactat gcgaaaacct ctttaaacta cgtcaacttt accttaccta cagcttcaaa
      421 aggacgtttt gagtcatctg ctggtcgatt tattcattga ttttcaatct tctaatacta
      481 aacaaaaatc aaacaactat agtgaaataa cataaaattt acatgaatag attagggaaa
      541 tcaaagcaag ttctaggaat gttttagcag tcaccgtata ctttcccagc ctcaatccac
      601 tataactcta cacataaaaa tggagaagat ggccatcctc ttctccaaat attaacttct
      661 ttacaaacca actatagttg acaaagaacc taaaatcaat tgataacaca aggtcaggtc
      721 ggtcaactct ttcaactgaa gccctgtcaa ctcttcccat ttatcaatct tgtattggag
      781 agaattgcgg tgcagatata gttgctgggc tgttttagtg agaacaacac tattttccca
      841 aagagagaga atgatttcct gaatctgttc ttgatccaaa atcatctggt gtagacattc
      901 cttgatttgc ttcaagtcca cgagtctttc tcccatactc caaagataga gctgagaaaa
      961 agtatgaaca ccttggtgac cctgacgcca ccatgtcttg aacaaatccc gctcagcttt
     1021 gattaagtct gatagggctt gatgccccgt ctgagaccaa acctgaccca acatgataga
     1081 aagacgaagt ccaaagtcat actcaaccgc ttcaatcgta tcacttaaaa tatctcttac
     1141 agaagtgtat ttgtcttgtt gaagcacgaa aacataatcc tgagctccga cctgtagcac
     1201 tgtctgacaa ttcggaaaaa gagtccgcat catatctagc caagaagcca gattttcctg
     1261 ctgaaaataa gaaagatggc aataaactaa ctgaatcttt ttaaaaactt gcggtgcctg
     1321 tcccttgcct tcaaccagat aggaatacca agggtttagc gaacgagcct gctcctgctg
     1381 ggtcaaaagg gcaaccaact gcttttcacg ctcgctgagc ccagcttcct ccagcaaaat
     1441 ccactgctga gaagctaaag ggagcgtgag atagccctct ttctctactg gttggtctga
     1501 aatccgagcc tcaggaaacc agtcttgtag ttctttttcc atcatgtcct agacctccac
     1561 tttttggatg caccatgaaa ccaaactctc aagacgttcc agattctcag tcatatggag
     1621 ataacccatg actgcttcaa aacctgtgga catacgataa gtcaccacat ctgcattttt
     1681 agcctttgtg tgactattag tattgcggcc acgtttgtag atttcttctt ctttttccgt
     1741 tagaacctgc tcctccaaca tgagagcaat caggcgagcc tgagccttgg ctgacacata
     1801 cttggttgct tcttgatgga gtttattggg tttggtcata cctttgagga tgaggtgacg
     1861 gcgaatatac atagaataca ccgcatcccc ctcaaaggct agcgcaatcc cgttaatgag
     1921 attgacatca atcacgtgtc cacctcactc catccttggt atcaaggagc ttaattcctt
     1981 gagtaaccaa ttggtcacgg atttggtctg ctgtcgcaaa gtcacgattg gcacgcgcct
     2041 cttggcgttt ttgaatcaag tcttcaatct ctgcatccaa aacttcctca acaaagacaa
     2101 ttccaaaaac ttctaacata tccgcaagag cttgcttgac acttgcatca tagttccctg
     2161 agttgctcca tttggccatt tcaaagacaa ctgtgatacc gttggcagca ttaaaatctt
     2221 catccatagc tgctacaaac ttatctttaa agttttgtaa ctcttgggca tccacatttc
     2281 ctgtaaatgg ttgttcgtaa gtattcttca gatacttgag attggtctcg gcatcgcgaa
     2341 ctgccttttc cgtgaagttg ataggcttac ggtagtgctg ggtcgcaaag aagaaacgaa
     2401 gtacttgccc atcaagagtt ttaagggcat cgtgtaccgt aatgaagtta cccaaggact
     2461 tagacatttt gatattgtcg atattgacaa agccattgtg catccagtag ttagcaaaag
     2521 ccttgcctgt tttagcttca gactgggcaa tttcattggt gtggtgtgga aactctaggt
     2581 cagctccacc accgtggata tcaatggtat cacctaaaat ctctgtcgac atgactgaac
     2641 actcaatatg ccaacccgga cgtccaggtc cccaaggact atcccaagaa atctcacctg
     2701 gtttggaaga tttccataga gcaaaatcta caggattttc cttacgagcc gtttcttcat
     2761 cggtacgacc tgaagcacct agatccaaat cttccaaggt tttattagct aatttagcat
     2821 agttgtggga tttttctaca cggaaataga catccccttg actctcatag gcaaagcctt
     2881 tctcgatcaa gtcttccaca aaacggatga tgtctgccat aaactccact acacgcggat
     2941 ggcgagtcgc aggtttcacg cctaaggccg tcacatcctc acgaaaggca gcgatgtact
     3001 tatccgcaac ctcctgaggc gtgatacctt cttccctggc acggttgata atcttatcat
     3061 ccacatctgt aaaattggaa atataggcaa ccttataccc acggtactca aaatagcgac
     3121 gaatcgtatc aaaagctacc gtcgaacggg cgtttcccac gtggatatag ttgtacaccg
     3181 ttggcccaca aacatacatc ttgatcttgc cgtcctcaat cgggacaaat tctcgcaaat
     3241 cacgagacat ggtgtcatag attttaatca taaatcataa tcaagaaagc tgaaatccaa
     3301 gaacaattag tttcatcact aaaagttcaa gtaaatttca gtccgaatat ctctacactt
     3361 cggaatccct tgctcctttc tcattcagat aaaccacctg agtctgtttg acaaagccaa
     3421 ttttttcata caaacgtttg gcacctacat tgctatcttc cactgcaatc tgaaattcct
     3481 tgtcattttg ctcaattagt tggttgacga gggattttgc taagtagctt ccatagcctt
     3541 tcccacgttt aggttccgat attgctaaac cgtagaagta attcgtatta gtcgataaat
     3601 ctaccgtgca agtcccaata acctgaccgt cttttaacaa aatatatagt cggctttctg
     3661 gatccttcag agcttcagcg acatatctat ccacaacttc tctcgattca tgttcctctg
     3721 aaaatgcctg aaattttaat tgactaattt gatcctgata cgaactatct gctaacaaaa
     3781 cttcaagatg ggaaacattt gctaacggat aaagtcttct atccttacct aaccaagttt
     3841 ctgtctcttc atcctcgatt agtccccagt tactggcaaa gtcaggatga ttctctaaaa
     3901 aaatacgttc tgtctgaaaa gtgactgatc gaatggggaa agaagctgtt tctctctcaa
     3961 aactagtaaa caatgcacgc gcaatcccct gacggcgatg acctggatga accagtatcg
     4021 tcacttctac atcttggtca tctgcataga cagttaataa accaacaagt tcgccttttt
     4081 cataataaag gaaaaaggcg ggcatgtttg ggtcaaaatt aagcatgtta gagagatagg
     4141 gatcgcgata ggtaccgtca tagttttggc aacagttaat tacttttttc gcctcagata
     4201 gctcctcttg gcttaacttg tttcttactt gaatcatata ggtatcctct acaaaccaga
     4261 cgatctgtga ctggcatctt tagcctgctc gagtttattg acataatact ctcgtttttc
     4321 ttcgacttcg tgaatgacag gctcatcttt cttaccatga agacggacaa tcttggccgg
     4381 aataccgaca accgtcacat cactaggtac atctgctacg acaactgctg cagcaccgac
     4441 cttggcattt tcaccaattt ccacaggtcc gataacttgg gcatgggctg atatgagggc
     4501 tccctttcgt acagtcggat ggcgtttgcc acagtctttc cctgttcccc caagagtcac
     4561 tccgtgatag agaagaacgc ctttttcaac aatcgctgtc tctccaatca ccagaccaga
     4621 accatggtca ataaagacac ctgaatcaat ctgggctcct ggatgaatct caatctgagt
     4681 ccaaaagcgc caaaactgac tgtgcatacg agctaagagt ttgaagccgt gcttccagag
     4741 aaaatgcgag agacggtggg ccgccaaggc cttgacacct ggataagtca gcaaaacctc
     4801 caaagtggtg cgggccgctg gatcattttc ttttacgata tcaatggttt cgcgccacca
     4861 ccccatacat ttctcctttt cttattctga atcttttggt gtttctgtaa attctttctt
     4921 aggtttgtga tccttttgat gacgtgggcg gtgagggcgc tcagactttt cacctttttc
     4981 atcatgctca ggttttggcg aacgaggtag aagagccttc atagaggcat cgatacggcc
     5041 tttttcatca atcttgataa ccttaacatc aacttcatcc ccgatttcta ccaaatcctc
     5101 tacacgattg gtacgagtcc aagccatctc agagatatga acaagggcat ctgtcttatc
     5161 aaagaggtta acaaaggcac caaatttctc gatacgaacg actttagcac ggtaaacttc
     5221 atccactttg gcttcacgaa ccaaaccagc aataatttct ttggcacggt taatagcatc
     5281 ttggtcacta gagtagatag acacatttcc ttcttcgtct atatcaatct taacacctgt
     5341 ttcagcgata atcttgtcga tggtttctcc acccttaccg atgacaatct taatcttgtc
     5401 cacatcaatc ttgatcgtat caattttcgg agcagttgga gccaattctg gacgaacttc
     5461 tggaatggtt gcttcaatga catcaaggat ttcaaaacgc gctttcttgg cttgagcaag
     5521 agcctccgtc aagatttctg cagtaatccc ttgaatcttg atatccattt gaagggctgt
     5581 aatcccatca cgagtacctg caaccttgaa gtccatatct ccaaagtgat cttccaaacc
     5641 ttggatatct gtcaatactg tgtagttatt tccatctgag ataagtccca tagcaatacc
     5701 agctactggc gccttgattg gcacaccacc agccataagg gcaagagttc ccgcacagat
     5761 agaagcttga gatgaagaac cgtttgattc caaaacttct gctactagac ggatagcgta
     5821 tgggaattct tccaagcttg gtaagacttg agcaagagca cgctcaccaa gagcaccgtg
     5881 accgatttca cgacgacctg gcgcaccgta acgacctgtt tcccctacag aatattgagg
     5941 gaagttatag tggtgcataa agcgtttctt gtactctgga tccaaaccat caatgatttg
     6001 agtttctccc atcggagcca aggtcaagac tgaaagagct tgagtttgcc cacgagtaaa
     6061 gagacctgaa ccatgtacac gaggaaggaa gtc
//
LOCUS       DAVKDZ010000103         5970 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000103 DAVKDZ010000000
VERSION     DAVKDZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.104"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            147..446
                     /locus_tag="VWZ30_001950"
     CDS             147..446
                     /locus_tag="VWZ30_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEU9335620.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            443..760
                     /locus_tag="VWZ30_001951"
     CDS             443..760
                     /locus_tag="VWZ30_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU9335621.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRDIYPALPEIEE
                     TNPEFTFIRMGRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            776..1402
                     /locus_tag="VWZ30_001952"
     CDS             776..1402
                     /locus_tag="VWZ30_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007557121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEU9335622.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVACLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMIPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDSAKHWTV"
     gene            1444..2205
                     /locus_tag="VWZ30_001953"
     CDS             1444..2205
                     /locus_tag="VWZ30_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9335623.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEALKA
                     EFAETFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALGLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMVKANQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTRQPRRVQITDMTIMANQQATGFMIHKK"
     gene            2283..2678
                     /locus_tag="VWZ30_001954"
     CDS             2283..2678
                     /locus_tag="VWZ30_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU9335624.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRPRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            2948..3232
                     /gene="groES"
                     /locus_tag="VWZ30_001955"
     CDS             2948..3232
                     /gene="groES"
                     /locus_tag="VWZ30_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU9335625.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            3248..4870
                     /gene="groL"
                     /locus_tag="VWZ30_001956"
     CDS             3248..4870
                     /gene="groL"
                     /locus_tag="VWZ30_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU9335626.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGSATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(4970..5970)
                     /locus_tag="VWZ30_001957"
                     /pseudo
     CDS             complement(4970..5970)
                     /locus_tag="VWZ30_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1799 a         1114 c         1409 g         1648 t
ORIGIN      
        1 acaacctcaa aacagtgttt tgagcagcct gcggctagct ttctagtttg ctcttagcat
       61 aagaagtcct tttccttaaa ggaagaggct ttttacttgc ttttctgctg cttcctcatt
      121 tgtcttgaag ccttttttgt gttaaaatga atggtatgaa agctaaaaaa atgtggatgg
      181 caggcttggc tctgctaggt atcggaagcc ttgctcttgc tacgaaaaaa gttgcagatg
      241 accgtaagct catgaagact caggaagagt tgacagagat tgtgcgagac catttttccg
      301 acatggggga aattgcgacc ctttatgttc aagtttacga aagcagtctg gagagcttgg
      361 ttggtggcgt catttttgag gatggccgtc attatacctt tgtctatgaa aatgaagacc
      421 tagtctatga ggaggaagtc ttatgataca gccagcaagt ttagaagaat tagcatcttt
      481 agtggaaaaa gcgggcaaga aggtcttcct ttttgtggca gactggtgtg gcgattgtcg
      541 tgatatttat cctgccttac cagagattga ggagaccaat ccagagttca cctttattcg
      601 aatgggccga gatcagtata tggatttggc caaactctgg gatgtttacg gaattcctag
      661 ccttgttgtt ctagaaaagg acaaggaaat cggtcgtttt gtcaatcgcg accgtaaaag
      721 taaggagcaa attaacgatt ttttagcagg attgaaatag gagaaaaagg aaacaatgat
      781 ttttacatat aacaaagaac atgtcggtga tgtccttatg gtcatcgtga aaaatagcgg
      841 agatgccaaa ctgaatgtgg aacgcaaagg caaggtagcc cgtgttttcc tcaaagaaaa
      901 tggggaaaca gtagcttgga acatttttga ggtttcgagc ttgtttgaaa ttgcagagcg
      961 aggtcaagtc tttttatcag atgagcaagt cgcttgtttg aaccaagaat tacaggcgga
     1021 aggttttaca gaagaaattg tcaatgacaa ggaacctaag tttgttgttg gtgaaattgt
     1081 tgagatggta gcccatccag atagtgacca cctcaacatc tgccaagttg cagtcgcaag
     1141 tgataagact gtacaaattg ttgcaggagc tcccaatgcg cgtgtggggt tgaaaaccat
     1201 tgtcgcgctt cctggagcta tgataccaaa agggaatctc attttcccag gtgaacttcg
     1261 tggtgaaaag agttttggta tgatgtgcag tcctcgtgaa ttgcatctgc caaatgctcc
     1321 acaaaaacgt ggggtgcttg aattatcaga agaccaagtt gtcggaacac catttgactc
     1381 agctaaacac tggactgtct aagaagttgt taatatctga tagaccagct agaaggaaat
     1441 aagatggcaa aaaatgttgt gattacagga gcgacatcag gaatcggtga agcgattgcg
     1501 cgtgcttatc tagagcaggg tgaggatgtc gttctaacag gacgacggat agacagacta
     1561 gaagctctta aggcggagtt tgcagaaact tttccaaatc aaacagtttg gacttttcca
     1621 ctagatgtga cggatatggt catggtgaag actgtttgct ctgatattct agaaacgata
     1681 gggaggattg atatcttggt caacaacgcc ggactggctc ttggcttagc tccctatcaa
     1741 gactatgaag agttggatat gctgaccatg ttggatacca atgtcaaagg tttgatggca
     1801 gtcactcgct gtttcttgcc agcaatggta aaagccaatc aaggtcatat tatcaatatg
     1861 gggtcaaccg caggaattta tgcctatgct ggtgcagctg tttactcagc caccaaggcg
     1921 gcagttaaga ccttttcaga tggactgcga attgatacca tcgcaacgga tatcaaggtg
     1981 acaaccattc agcctgggat tgtcgaaaca gatttctcaa ctgttcgttt tcatggtgat
     2041 aaagagcggg ctgcgtccgt ttaccaagga atagaagcct tgcaagctca ggatattgca
     2101 gacacagtag tctatgtgac cagacagcct cgccgtgttc agattacaga tatgaccatt
     2161 atggctaatc aacaggcgac aggtttcatg attcataaaa aataagaaat ttcctcgaaa
     2221 agttacaaat ttctgtaact ttttttgatt tcctacgaat agataagtag gaggaagaaa
     2281 atatgtataa taaagttatc atgattgggc gtttaacgtc tacaccagaa ttgcacaaaa
     2341 ccaacaatga caagtcggta gcgcgagcaa ctatcgctgt caaccgtcgt tacaaagacc
     2401 aaaacggtga acgtgaagct gattttgtca atatggtcct atggggcaga ctagcagaaa
     2461 ctttggcaag ctacgcaacc aaaggtagtc tcatttccgt tgatggagaa ttgcgtcccc
     2521 gtcgctttga gaaaaatggt cagatgaact atgtaaccga agtccttgtg acaggattcc
     2581 aactcttaga aagtcgtgct caacgtgcca tgcgtgaaaa taatgcaggc caagatttgg
     2641 cagatttggt cttggaagag gaagaattgc cattttaata ctcttcgaaa atctcttcaa
     2701 accacgttag ctttatccac aacctcaaag caatgctttg agcaacctgc ggctagcttc
     2761 ctagtttgct ctttgatttt tattgagtat aaacattaaa aagtctgagt tggtctcagg
     2821 ctttttatct tgagaaagtc agactttttt cttgactatt tctgaccaag tgatacaata
     2881 gaattatgaa ttagcactca agttcaaaga gtgctaataa tatctatctc attatggagg
     2941 aaatcagatg ttgaaaccat taggggaccg tgtggtctta aaaatagaag aaaaagaaca
     3001 aaccgttggg ggctttgtcc ttgcaggatc agcccaagaa aaaaccaaaa cagctcaagt
     3061 tgtggctact ggacaaggtg ttcgtacctt gaacggtgac ttggttgctc caagtgttaa
     3121 aactggagat cgtgtcttag ttgaagccca cgcaggtctt gatgtcaaag atggcgatga
     3181 aaagtacatc atcgtaggcg aagctaacat tttggcaatc attgaggaat agaaggagaa
     3241 agtaagtatg tcaaaagaaa ttaaattttc atcagatgcc cgttcagcca tggttcgtgg
     3301 tgtcgatatc cttgcagaca ctgttaaagt aaccttggga ccaaaaggtc gcaatgtcgt
     3361 tcttgaaaag tcattcggtt cacccttgat taccaatgac ggtgtgacca ttgccaaaga
     3421 aatcgaattg gaagaccatt ttgaaaatat gggtgctaag ttagtatcag aagtagcttc
     3481 taaaaccaat gatatcgcag gtgacgggac tacgactgca acagtcttga cccaagctat
     3541 cgtccgtgaa ggaatcaaaa acgtcacagc aggtgcaaat ccaatcggta ttcgtcgtgg
     3601 gattgaaaca gcagttgccg cagcagttga agctttgaaa aacaacgcca tccctgttgc
     3661 caataaagaa gctatcgctc aagttgcagc cgtatcttct cgttctgaaa aagttggtga
     3721 gtacatctct gaagcaatgg aaaaagttgg caaagacggt gtcatcacca tcgaagagtc
     3781 acgtggtatg gaaacagagc ttgaagtcgt agaaggaatg cagtttgacc gtggttacct
     3841 ttcacagtac atggtgacag atagcgaaaa aatggtggct gaccttgaaa atccgtacat
     3901 tttgattaca gacaagaaaa tttccaatat ccaagaaatc ttgccacttt tggaaagcat
     3961 tctccaaagc aatcgtccac tcttgattat tgcggatgat gtggatggcg aggctcttcc
     4021 aactcttgtt ttgaacaaga ttcgtggaac cttcaacgta gtagcagtca aggcacctgg
     4081 ttttggtgac cgtcgcaaag ctatgcttga agatatcgcc atcttaacag gcggaacagt
     4141 tatcacagaa gaccttggtc ttgagttgaa agatgcgaca attgaagctc ttggtcaagc
     4201 agcgagagtg accgtggaca aagatagcac ggttattgta gaaggtgcag gaaatcctga
     4261 agcgatttct caccgtgttg cggttatcaa gtctcaaatc gaaactacaa cttctgaatt
     4321 tgaccgtgaa aaattgcaag aacgcttggc caaattgtca ggtggtgtag cggttattaa
     4381 ggtcggatcc gcaactgaaa ctgagttgaa agaaatgaaa ctccgcattg aagatgccct
     4441 caacgctact cgtgcagctg ttgaagaagg tattgttgca ggtggtggaa cagctcttgc
     4501 caatgtgatt ccagctgttg ctaccttgga attgacagga gatgaagcaa caggacgtaa
     4561 tattgttctc cgtgctttgg aagaacccgt tcgtcaaatt gctcacaatg caggatttga
     4621 aggatctatc gttatcgatc gtttgaaaaa tgctgagctt ggtataggat tcaacgcagc
     4681 aactggcgag tgggttaaca tgattgatca aggtatcatt gatccagtta aagtgagtcg
     4741 ttcagcccta caaaatgcag catctgtagc cagcttgatt ttgacaacag aagcagtcgt
     4801 agccaataaa ccagaaccag tagccccagc tccagcaatg gatccaagta tgatgggcgg
     4861 gatgatgtaa gctttctata gaaaacaact tataaaaaac acaaaaggag ggaatgacta
     4921 acccttcttt ttataggctc tttgtcaact gtagtgggtt gaaaaaaagc taagcttgag
     4981 aaaggacaaa tttcgtcctt tcttttttga tattcagggc gataaaaatc cgttttttga
     5041 agttttcaaa gttccgaaaa ccaaaggcat tgcgcttgat aagtttgatg agattattgg
     5101 ttgcttccag tttggcatta gaatagtgta gttgaagggc gttgacaatc ttctctttat
     5161 ctttgaggaa ggttttaaag atagtctgaa aaagaggatg aacctgcttt agattgtcct
     5221 caatgaatcc gaaaaatttc tccggttcct tattctgaaa gtgaaacagc aagagttgat
     5281 agagatggta gtggtgtttc aagtcttctg aatagctcaa aagcttgttt aaaatctctt
     5341 tattggttaa gtgcatacga aaagtaggac gataaaatcg cttatcactc agtttacggc
     5401 tatcctgttg aatgagtttc cagtagcgct tgatagcctt gtattcatgg gattttcgat
     5461 gaaactgatt catgatttgg acacgcacac gactcatagc acggctaaga tgttgtacaa
     5521 tgtaaaagcg atcaagaacg attttagcat tcgggagtga aacagtctgg gagactgttt
     5581 cagcctgagc ctagaaattt gcaagcgaag ctgtttagcc aagtcatagt aagggctaaa
     5641 catatccata gtaatgattt tgacgcgaca tcggacggct ctatcatatt taagaaagtg
     5701 atctcggatg accgcttgtg ttctaccttc aagaacagtg atgatatcga gcttttcaaa
     5761 atcttgcgca ataaagctca tctccatctc ccgattgaaa cagtcactcc ccggactgtt
     5821 tcaacgtccc aagacataat ctcaggcaga tttctaaaat tacactcaaa gtggaagtca
     5881 ttgagctttc gaatgacagt tgaagttgaa atggccagct gatgggcaat atcggtcata
     5941 gaaatcttct caatcaactt ttgcgcaatt
//
LOCUS       DAVKDZ010000104         5891 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000104 DAVKDZ010000000
VERSION     DAVKDZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.105"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..143
                     /locus_tag="VWZ30_001958"
     CDS             <1..143
                     /locus_tag="VWZ30_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU9335627.1"
                     /translation="YVTENSSAHLHMYGKIEAKHNRKMGHVTLFSDVPDSVEEFGEGI
                     DF"
     gene            153..380
                     /locus_tag="VWZ30_001959"
     CDS             153..380
                     /locus_tag="VWZ30_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEU9335628.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            443..1741
                     /gene="purB"
                     /locus_tag="VWZ30_001960"
     CDS             443..1741
                     /gene="purB"
                     /locus_tag="VWZ30_001960"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU9335629.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(1796..5731)
                     /gene="strH"
                     /locus_tag="VWZ30_001961"
     CDS             complement(1796..5731)
                     /gene="strH"
                     /locus_tag="VWZ30_001961"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9335630.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTTTTENQ
                     PTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEVS
                     STPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVNSKESNTDKTDKDKPAKKDEA
                     KAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTDL
                     HLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTDL
                     INYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKESACTVDLGNEQAVA
                     FTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYEK
                     FIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVASS
                     KLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIPI
                     IGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRYT
                     AESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKRE
                     VEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTIT
                     ANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSPG
                     HMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKIF
                     NFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYED
                     KDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQDEAYP
                     LSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRQSAEYKEEEIFELMTAFAD
                     HNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTLV
                     ANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAGQ
                     ENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESGL
                     APTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADGK
                     EVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPAE
                     ESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
BASE COUNT         1498 a         1172 c         1313 g         1908 t
ORIGIN      
        1 aatatgtgac agaaaattca agcgcccacc tccacatgta tggtaaaata gaagcaaagc
       61 ataatcgtaa gatgggacat gtgactttgt ttagtgatgt gccggatagt gtggaagagt
      121 ttggggaagg gattgatttt taggacaagt ctatgataca aattatcgtt aatacattta
      181 ttgaaaagga taagactgga gcagttgttg aagtgttgta tgccagtgct gaccaagata
      241 aggtacaagc taaatatgaa gaactagctg cacaataccc cgaaaattat ttagctatct
      301 atgatgtacc gctggatacg gatttgaata cactagatca ttacccgtct gtgtttattg
      361 aaaaagagga gtttgagtag aaatcttggt ttacctagat agcttattcc caacagctta
      421 agaagaaagg aaaaattaac acatgatcaa ccgttactct cgccctgaga tggcgaatat
      481 ttggagtgaa gaaaataaat accgtgcttg gcttgaggtg gaaatcctct ctgacgaggc
      541 atgggctgag ttgggggaaa tccctaagga agatgtggct ttgattcgca agaaggcgga
      601 ctttgacatc gaccgtattt tggaaattga gcaggagacg cgccacgatg tggtggcttt
      661 cacgcgtgcg gtttctgaga ctcttggtga agagcgcaag tgggttcact atgggttaac
      721 ttctaccgac gtggtggata ctgcttatgg ttacctctac aagcaggcca acgacatcat
      781 ccgtcgtgac cttgaaaact tcactaatat catcgctgac aaggccaagg agcacaagtt
      841 caccatcatg atggggcgta ctcatggtgt gcacgctgag ccgacaacct ttggtcttaa
      901 attggcaact tggtacagcg aaatgaaacg caatatcgag cgtttcgagc atgcagctgc
      961 tggtgtagaa gctggtaaga tttctggtgc ggttgggaac tttgccaata tcccaccatt
     1021 tgtagaggag tatgtctgcg ataaacttgg catccgtgcc caagaaatct ctacacaagt
     1081 ccttcctcgt gaccttcacg ctgagtactt tgcggttctt gccagcattg cgacttcaat
     1141 cgaacgtatg gcgactgaga ttcgtggtct acaaaaatct gagcaacgcg aagtagaaga
     1201 gttctttgct aaagggcaaa aagggtcttc agcaatgcct cacaaacgca acccaatcgg
     1261 ttctgaaaat atgactggtc tggcgcgtgt cattcgtggt cacatgatta cggcttatga
     1321 aaacgtcgct ctctggcacg aacgcgatat ttctcactca tcagctgagc gtatcatcac
     1381 accagatacg accattttga ttgactacat gctcaaccgt tttggaaata tcgtcaagaa
     1441 cttgacagtc ttcccagaaa atatgatccg aaacatgaac tcgacttttg gtcttatctt
     1501 tagccaacgg gctatgttga cattgattga aaaaggcatg acccgtgagc aagcctatga
     1561 cttggtgcaa ccaaaaacag cctactcttg ggacaaccaa gtagacttta aaccacttct
     1621 tgaggcagat tcagaagtaa catcacgtct cactcaagaa gaaatcgatg aaatcttcaa
     1681 cccagtttat tacaccaaac gagtggatga tatctttgaa cgtcttggac taggtgatta
     1741 attaaaaaat aaacagcgag cttcaatctc gctgtttatt ttttatcgaa aagacttagt
     1801 cttcttttct tttagtgagt ccataggctg ctagtgtgga catgagtcct gcgactacta
     1861 gtcctgcaga atcgtgagtt cctgtttcag gaagtttttt ctctgttacc acaggagctg
     1921 gatcttgagg aagagctttg ctttcctcag caggagcagt tgatggagct ggttggcttg
     1981 ggatttctag ttttggtttt tcttcagcaa tagcggcttg tccgttttca tcgcctacat
     2041 gtgttaccat agttccgact tcgactattt gagtaacggc ttcctgtgct acgacactat
     2101 ttacaagtgt tttcacttcc ttaccatcgg cagaagtgct cacagagtag aagttgctac
     2161 gatgtccatt gacgccctta gtaatgactt gtgtttttcc tttgagtaag agtggatttt
     2221 cacgagtcac tgtggtaaat ggaatttctt ctttttggat atccagtcta ggttttacct
     2281 cagtagttgg tgcaagacca ctttcatcac ccttgtgagt tacaggagcg ccaacttcaa
     2341 ccacttggtt tataacttct ttggttacct ggctatcaag gactgtttct gttgtttttc
     2401 cattttcagt gagtacagag atgtaatgag ttcgttcacc tttgactcct gctgtgataa
     2461 tattttcctg accagctggg aggttaggat tttctttctt gataacttca aatggaattt
     2521 cttcagttct tgtgatgagt tctggtctgg tttcaacatt ggcagccact tcattttcat
     2581 ctaggcttcc tgaatgagtt gcagctggtt tgaggccttg aagagcggct tttaggttgg
     2641 ctacaagtgt gtcaagctca gcttgtttat tacggttgag gttgtaattt agagctgttt
     2701 tagctgcgtc aagggcctca agactttctt tactatatcc ttctaagttt gtaggaattt
     2761 tagctaattc ttcgcggaga gcattataat tagcacggaa gtagtctttg ttgtggtctg
     2821 caaaggcagt catgagttca aagatttcct cttccttgta ctcagcgctt tgtctatctg
     2881 cccagattga aagcatactt ccgactgttg gaagatctac ttcaggatat ttggtagaag
     2941 ctagttgatt gaatggaact ttaccagaat tttcaactgc ttttgaaaga gggtaagctt
     3001 catcttgttt gtgattgcct ataacataat accagtcacc gttggtattc aagaacttat
     3061 aacccttgct tgctaggtat tgaggtgatg cgaggttgta tccccaccag cctttagacc
     3121 agtaagaaat caagacatct ttgtcaaact gaacatcgtc cttgtcttca tagtagaagc
     3181 catcgttgaa ggccattggt tgaagccctc tttctttggc catagctgcg agggtgttgg
     3241 catattcggc aaatttgcca tagagttgat accacttgag gtagtaccag ccttgggcac
     3301 tagtcgcatc gttggcgtat tcgtcagtac caaagttgaa aatctttgtt ttacctgcaa
     3361 agaagtccat gtatttaccg atgagggctt ttacaaagtt catcgcttct tcgtttttca
     3421 agtccatagt tgtttttgaa actttatcaa agtgggcttg aggattttta atacctaatt
     3481 tttccatggc aaccagcata gcatccatgt gacctggact gttaatagct gggatgagac
     3541 caatgtcctt agatttagcg tattcaatta gctctgtcac ttctgcctgt gttagtgcag
     3601 taccgtttgg atcgtcgtag taagctttag ttccttcgat aatagctttt ttaacgtcat
     3661 cactagcata ggtttttccg ttggcagtaa tggtcatatc attgagtaga aagcgaagtc
     3721 cgtcatttcc tagaaggaga tggacatcag aatatccgag ctcactggcc ttgtctacga
     3781 tgcgtttgag ctggttcaga gtaaagtatt tgcgtccagc atcgattgag attaccttgt
     3841 ttttggcaag tttttcaacc tcacgtttag cttcttcttc tttttgagct tcaggcgtga
     3901 gggtcaagtt gttgacagtt tcttgaagtt tagcaatggc ttgatcaatc gtatcttgtt
     3961 gggcacggct aaggttgcta tcgagagagc gaatagcttt ttcagcttct tttacggccg
     4021 tgacgctttc tgcagtataa cggttcaggt cttttggtac ctcgttaagt gcttgctctg
     4081 cagattcata atcagctgcg aagtattcag cgttggcatt tgcaaaatga cgcatgagtt
     4141 tgaagaggcg tgatggtgaa taacgtgcag atggagtgtc agcccaagca gctaccatac
     4201 caccgatgat tgggatatca gctccttctg tttttggtac agaagtgatt ggtgtgtttt
     4261 taataccatt gagcccctga tcgagattgt accagccttg gccatcagcg tttcgtccaa
     4321 gaacgtagta ccaagcatca ttggtattaa ggatttggtg acctttttca gctagtagtt
     4381 tagaagaagc gacatcgtag cctccccaac caccagtcca catagaaacg atgatgtctt
     4441 tgtcaaaact accaaagctt gtgtcgctat tgtagtagat accgtcgtta aaggccattg
     4501 gtttgagacc gtgcgatttt acaatacgag cgaggtcatt ggcgtaggca ataaattttt
     4561 catagccttt tacagggtag ccttcgtttg gatagtattt atcagcttga agcacactcc
     4621 aacctttagc atctgtcgca tcattggcat attcatcaag tccgatgttg aagatttcag
     4681 tctttttcgc gaaataagca gcatacttgt cgataagggc ttttgtaaaa gcgacagctt
     4741 gttcgttgcc aagatcgaca gtacaggctg attccttccc aaaatagcta aagttagggt
     4801 tttggattcc caattctttc atggcattga gaatcgcatc catgtgacca ggactattta
     4861 ctgtcggaat gagaccgata cctttatctt ttgcatagtt aatcagatct gtcatttgac
     4921 tttctgttaa gtgattgccg tttggatcgt tgtaataatc atttgtacct ttttcaatgg
     4981 cgcgtttgac atcgtcactg gcataggtct tgccgttagc tgtgatgctc atatcgtcca
     5041 acatgaaacg gagtccatca tttccgacta ataggtgtaa atcagtgtag ccataatgtt
     5101 tcgctttatc gatgatttcc ttgagctgtt ctggtgagaa atatttacgt ccagcatcaa
     5161 tagaaacaat tttctttttc gctagttttt catttacagt tgcagcacgt tcctttcctg
     5221 cctctgtttc cggtttgtca gcctctgctt tcgcttcatc ttttttagct ggtttatcct
     5281 tgtcagtctt gtctgtattt gactctttag aattaacctc tttcgcttct tcctttttag
     5341 ggctagcttc ttctgccttt ttattagcag tttctttttc agcagaagtt ggagttacca
     5401 cctctgcttt atcactagga gttgaactaa cttcctcttg tggtttttct tctgtttttg
     5461 gaagactagc taccttatca gtagctggag tttctgtttc tacagttttt ggagcttctg
     5521 gttgaagctc tgctttaggt gtttcctcag tccgattttc ggatgattga ggggaatcag
     5581 aaactgtatg gatggtcggt tggttttctg tagtagtagt aactccatcg gctgcaacag
     5641 tctgtgcttg gaaggcaaat ccaattagaa cagaagctgc tcctacagcg tatttacgaa
     5701 tagaaaaacg ctgttgtttt tcatgtttca ttgcaaaacc tcctgattgc attgttatat
     5761 tgatagcgat tatataaatc aacgccctta ttttatttct tatattaacg agagtcaaga
     5821 ggagatgaca aaaaactata ataagtataa aaaaatataa aatttaaact taagatttca
     5881 gattggtcgg a
//
LOCUS       DAVKDZ010000105         5866 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000105 DAVKDZ010000000
VERSION     DAVKDZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.106"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(51..680)
                     /gene="nth"
                     /locus_tag="VWZ30_001962"
     CDS             complement(51..680)
                     /gene="nth"
                     /locus_tag="VWZ30_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU9335631.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            complement(680..1222)
                     /locus_tag="VWZ30_001963"
     CDS             complement(680..1222)
                     /locus_tag="VWZ30_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU9335632.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVGDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            1392..1709
                     /locus_tag="VWZ30_001964"
     CDS             1392..1709
                     /locus_tag="VWZ30_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9335633.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYEHVKHVLSLYGPV
                     FGF"
     gene            1948..2256
                     /locus_tag="VWZ30_001965"
     CDS             1948..2256
                     /locus_tag="VWZ30_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9335634.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKVPEDLKKKLYTTTSF
                     "
     gene            2304..3506
                     /locus_tag="VWZ30_001966"
     CDS             2304..3506
                     /locus_tag="VWZ30_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9335635.1"
                     /translation="MHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLDEPS
                     NDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLVEHL
                     DYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGRLLA
                     KKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLSIDD
                     RVLAQELRLTVRGQEKIGIIGPNGVGKSTLLAKLKQFLNDKREISLGFMPQDYHKKLQ
                     LDLSSIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDLVLR
                     KPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSSIYRLTEHGL
                     ELVNLEDV"
     gene            complement(3550..4449)
                     /gene="htpX"
                     /locus_tag="VWZ30_001967"
     CDS             complement(3550..4449)
                     /gene="htpX"
                     /locus_tag="VWZ30_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU9335636.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(4451..5011)
                     /locus_tag="VWZ30_001968"
     CDS             complement(4451..5011)
                     /locus_tag="VWZ30_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU9335637.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPNNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            5105..5818
                     /gene="rsmG"
                     /locus_tag="VWZ30_001969"
     CDS             5105..5818
                     /gene="rsmG"
                     /locus_tag="VWZ30_001969"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU9335638.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFECYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
BASE COUNT         1858 a         1260 c         1095 g         1653 t
ORIGIN      
        1 tatagcacaa agcatgctta aaatcaagca aaaagtttca atgtagcatc ttacaaattg
       61 ctaaaatcat ataattgtgg gtactggtca cactctggat tttttggatg gcaaatggct
      121 cttccaaaat aaatcatggc ctgatgggca gctaaccact gctcaggcgg caagatatcc
      181 atgacccgct tttccacctc aagtggcgtc gctgattttt tgacaatatc gtggtgtttg
      241 caaatacgct ccacatgagt atccactgca aaggctggaa tcccaaatcc tacactcatg
      301 acaacattgg ctgtcttgcg accaacacct gccaaactct ccaattcttc acgtgtctga
      361 gggacttgac catcaaaatc gtctagtaac tgttgggcac attttttaag gaatttagct
      421 ttattccgat acaatcccaa gcgagaaata tgtgaagcaa tctcactctc tgtcgctaca
      481 gacatagctt ggggtgttgg aaaggcaaca aagagacctg gtgtggcctt atttaccgct
      541 gcatctgtcg tctgggctga taacatgacc gcaaccagga gttcaaaatg attggtaaaa
      601 tcaagactag gcttggcatc tgggaagagg gcaatgattt cttctagcac ctttcgtgct
      661 cgtttttttg acaagaccat tattcatctc cgtcaaatag tccttgtaag ccagcaaaag
      721 gactgttttc ttctttcttt actgcttgtt gagcttggta ttcttcctct gtcatgattt
      781 gccagtcatt tcctgagaca aatccttgac cagcttcttc ttcagccgtc aagaccttga
      841 taggaatgtt tagcaggata ttgtctgata cactctcagc aaggtcaagc gccccatttt
      901 cgatgggcaa gaccaagtca tcatctaaaa cttcttgatc tagctggtta gttgcgcctt
      961 ccatgaaaac ttccgtgact ggataagatt caactaactc aactggctcc atactgcgac
     1021 tcgaagcaag aacaatggta taagatagtt gataatctaa gaaatacata cggtcttcat
     1081 attgtacttt cccaactgca aggatatctt ttacatctaa aatttcttga ttacgtgcac
     1141 gcaggtcacc aactaaatct aacgtttgtt caaagttcaa accttcagac tgcttacgaa
     1201 tttcttgaat atttaatttc atacttcctc cataaagatt tactctcttg attataccat
     1261 gaaaaggcta caaatcagca caccaaactt tgtaattaaa attcaaaatt ttaacatatt
     1321 tactatgaca gttttgtttt ttagtgctat actataggga aagagtacat cagatcaagg
     1381 aggatgctca catggaagac aagaaactca ttcaactcct atccaagtta aataaaagct
     1441 accaaaactg taaacagggt acggcagatg atattcgact acaagagctg ctaaacacta
     1501 ctatgcaaga gctcaaaaaa acggaaaagt tgaacaacag tatcttaatt gatcttgaga
     1561 aattttacca acccaccagt cttctgattg gactgggtag cctaaaacta aacgatcaag
     1621 cacgcactgc ttggcgaaac tatgataaat tccattacga gcatgtcaaa cacgtactaa
     1681 gtctctatgg acctgttttt ggattttaga gcatagaatt tccagttttc tgttgacaaa
     1741 atttccttaa aggtataata taaagatact aatactcgga ggtaagggag acatgaacaa
     1801 ctaagtctat caaataaaga acctttattt agtagatctt gtttttgtct ctttttgtgt
     1861 gctcttttat gctctttttc tggcatgtta atagagtttt tttgacatag actttgggat
     1921 ctactaggta aagtagagct ttttgttatg cactatgaac attctagaaa gggaaatcat
     1981 atgataaaaa tcaatcacct gaccatcaca caaaacaaag atttacgaga ccttgtctct
     2041 gacctaagca tgaccataca agacggggaa aaggtagcta ttattggcga agaaggaaat
     2101 ggtaaatcaa ccttacttaa aactttaatg ggggaagatt tgtctgattt cactatcaag
     2161 ggagacatcc agtctgatta tcagtcactg gcctacattc ctcaaaaagt ccctgaggac
     2221 ctaaaaaaaa aactttacac gactacttct ttttagattc tattgattta gactacagta
     2281 tcctctatcg tttggcggag gaattgcatt ttgatagcaa tcgtttcgca agtgaccaag
     2341 agattggcaa tctatcaggg ggcgaagctt tgaaaattca gcttatccat gagttagcca
     2401 aaccctttga gattctattt ttagatgaac cttcaaatga cctagacctt gagacagttg
     2461 attggctaaa aggccagatt caaaagacca ggcaaaccgt tattttcatt tcccatgatg
     2521 aagactttct ttctgaaacg gcagacacta ttgtccactt gcgactggtc aaacaccgta
     2581 aagaagcgga aacgctagta gagcatttag actatgatag ctatagtgag cagagaaagg
     2641 ctaattttgc caaacaaagt cagcaagctg ctaacaacca aagagcctac gataaaacca
     2701 tggaaaaaca tcggagagtt aagcaaaatg tagaaactgc gcttcgagct accaaagata
     2761 gtactgccgg tcgcctattg gctaaaaaga tgaaaactgt cctctcacaa gaaaaacgct
     2821 acgaaaaggc agctcagtcc atgactcaaa agccacttga agaggaacaa atccaacttt
     2881 tcttttcaga catccaacca ttaccagctt ctaaagtctt agtccaactg gaaaaagaaa
     2941 atttgtccat tgacgaccgt gttttggctc aggaactgcg actaactgtc cgtgggcaag
     3001 aaaaaatcgg tattatcgga ccaaatggtg ttgggaaatc aactctgtta gccaaattaa
     3061 agcaatttct gaatgataaa agagagattt cccttggttt tatgccacaa gattaccaca
     3121 aaaaactgca attggattta tcctcaatag cttatatcag caaaactggg gaaaaagagg
     3181 aacttcagaa aatccaatct cacctagcca gtctcaattt cagttatcca gaaatgcagc
     3241 atcaaattcg ctccttatct ggcggacaac agggaaaact cctgcttttg gatttagtcc
     3301 tgcgcaaacc aaactttctc ctgctggatg aacccacacg aaacttttct cccacttctc
     3361 aaccccaaat cagaaaactc tttgcaacct atccaggcgg tctcatcact gtttcgcatg
     3421 accgtcgttt cttaaaagag gtctgttcaa gcatctaccg cttgacagaa cacggtttgg
     3481 agttagttaa tttagaagat gtataaattt gcaacatagc aaaaatccag agacgacctc
     3541 tggattcttt tacatctgtt ttaaacgttc aatccgttct gagataggtg ggtgggtata
     3601 aaagagtttt tggaaccccc cacctttctt gggatcattg atataaaggg cactgctagc
     3661 atcatcgacg tggcgactca taggtttgct attgtccaac ttatctaggg cattaatcat
     3721 tccctgggga ttgcgagtca gctcgacact agatgcatct gccagaaatt ccctctgacg
     3781 agaaatagcg agctgaacca aggttgcagc gagaggtgcc agtacaatag ctagtaggga
     3841 aaccactagc ataatgattt caagaccatt tccatctcgg tcatcatcac ttcgtctgcg
     3901 acctgctcca ccccaccaca tcatacgacc tgccatacta gaaagcatgg tgatagcact
     3961 agcaagggca actgcaatag tcgaaatacg gatatcataa ttacgaatat gactaacttc
     4021 atgtcccata acagcttcta gttcttcacg attcatgata gctagtagac ctgaagtcgc
     4081 agcaacagcc gcattttgag gattagaacc tgtcgcaaag gcatttaagg ctggatcatc
     4141 aatgatgaaa acacggggca taggaatctg agcgaccaga gccatatctt ccactacatg
     4201 gtagaggtct ggtgccgttt gctcatccac ctcacgcgct ccattcatgg acatgacaat
     4261 ctctgtcgat tgaaaaatca tagacaaagc gtagataaag ccgataatca gtgcgataac
     4321 caaaccacca agtccagatc ttataaagag ataaccaacc gcataaccaa caagagctaa
     4381 gagtaggaaa aataccagca acaaaatcca ggtttttcgt ttattgcttg caatttgatc
     4441 aaacaacatc ttagtcacct aaaccgctaa aatcaacttt aggaaccgac ttttcctctt
     4501 caggtgtttg aaggaaatct gccgctttaa atccaaacat tccagcgata atattgttcg
     4561 ggaaagtttc taattttaca ttgtagttgc tgacaacact gttatagagt tgacgagagt
     4621 aagaaatttt attttctgtg tttgtcaact cctcttgcaa tttaacaaag ttagcactag
     4681 ctttcaaatc tggatagctt tctgcaactg caaaaatacc tgaaacctga cgagtgaggg
     4741 catcactggc tttcatagct tctgctggtg aagtcgctgc cgccacttgg ttacgtagtt
     4801 ctgccacctt ttcaagggta gaaccttcat atttggcata accttttaca gtctcaatca
     4861 agtttggcaa gaggtcattt cgacgtttca actgaacatc aatctgactc caagcctcct
     4921 tggtttgcat acgattttta accaaaccgt tatagctaac aatcacaaaa ataacaataa
     4981 gagcgataac tccaagaata atccaagtca tgatataagt cctttctact tttagattag
     5041 taccagtata tcaaattttt aatgattgtg gtaaaataag atgatactaa agaaggaaat
     5101 aactatgaaa ccaaaaacat tttacaactt gcttgccgag cagaatcttc cactttcgga
     5161 ccagcaaaaa gaacaatttg aatgttattt tgagctcttg gtcgagtgga atgagaagat
     5221 taatttgacg gcgattacgg acaaggaaga agtttatctc aaacattttt acgattcgat
     5281 tgcacccatt cttcaaggtt tgattcccaa tgaaactatc aaacttcttg atatcggggc
     5341 tggggcagga tttcctagtc taccaatgaa aattctctat ccggagttag atgtgaccat
     5401 tattgattca ctcaataagc gcatcaactt cctacaactc ttggctcaag aactggattt
     5461 gaacggagtt catttctacc acggacgtgc cgaagatttt gcccaagaca agaacttccg
     5521 tgctcaatat gattttgtaa cagctcgtgc ggttgcccgt atgcaggtcc tatctgaatt
     5581 gactattccc taccttaagg ttggtggaaa actattggca ctcaaggcca gcaatgcgcc
     5641 tgaggagttg ttagaagcta agaatgccct caatcttctt tttagtaaag tcgaagacaa
     5701 tctcagctac gccctaccaa atagagatcc gcgttacatc acagtggtag aaaagaaaaa
     5761 agaaacacca aataaatatc cacgtaaggc tggtatgcca aataaacgcc cgctttaaat
     5821 ttttaagtaa aaaaatattt acaaaatcaa cctcgctctc ttattt
//
LOCUS       DAVKDZ010000106         5841 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000106 DAVKDZ010000000
VERSION     DAVKDZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.107"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            78..557
                     /locus_tag="VWZ30_001970"
     CDS             78..557
                     /locus_tag="VWZ30_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9335639.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            651..722
                     /locus_tag="VWZ30_001971"
     tRNA            651..722
                     /locus_tag="VWZ30_001971"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:684..686,aa:Glu,seq:ttc)
     gene            974..2519
                     /locus_tag="VWZ30_001972"
     rRNA            974..2519
                     /locus_tag="VWZ30_001972"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            2571..2643
                     /locus_tag="VWZ30_001973"
     tRNA            2571..2643
                     /locus_tag="VWZ30_001973"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2604..2606,aa:Ala,seq:tgc)
     gene            2768..5669
                     /locus_tag="VWZ30_001974"
     rRNA            2768..5669
                     /locus_tag="VWZ30_001974"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5746..>5841
                     /gene="rrf"
                     /locus_tag="VWZ30_001975"
     rRNA            5746..>5841
                     /gene="rrf"
                     /locus_tag="VWZ30_001975"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1723 a         1134 c         1667 g         1317 t
ORIGIN      
        1 ctaaagggaa cagaatggag aaaatgtaac aaatgtgttt tctaatctgt tagactgtat
       61 ctagaaaggg gaaaattatg attaaagaat tgtatgaaga agtccaaggg actgtgtata
      121 agtgtagaaa tgaatattac cttcatttat gggaattgtc ggattgggac caagaaggca
      181 tgctctgctt acatgaattg attagtagag aagaaggact ggtagacgat attccacgtt
      241 taaggaaata tttcaaaacc aagtttcgaa atcgaatttt agactatatc cgtaagcagg
      301 aaagtcagaa gcgtagatac gataaagaac cctatgaaga agtgggagag atcagtcatc
      361 gtataagtga ggggggtctc tggctagatg attattatct ctttcatgaa acactaagag
      421 attatagaaa caaacaaagt aaagagaaac aagaagaact agaacgcgtc ttaagcaatg
      481 aacgatttcg agggcgtcaa agagtattaa gagacttacg cattgtgttt aaggagttta
      541 ctatccgtac ccactaggaa gtcatgcaaa aaaaatgaaa aaaattagaa aaagtagttg
      601 acaaagtttg aaaaggctgt ataatagtaa gagttgaaaa taacaactca ggtccgttgg
      661 tcaaggggtt aagacaccgc cttttcacgg cggtaacacg ggttcgaatc ccgtacggac
      721 tatggtatgt tgcgtcagga ccacttgatg aaaaaaagtt taaaaaagct taaaaatctt
      781 caaaaaagtg ttgacaagtg aaagtggctg tgatatacta atatagttgt cgcttgagag
      841 aagcaagtgg caaagacctt tgaaaactga acaagacgaa ccaatgtgca gggcgctaca
      901 acataagttg tagtactgaa caatgaaaaa acaataaatc tgtcagtgac agaaatgagt
      961 aagaactcaa acttttaatg agagtttgat cctggctcag gacgaacgct ggcggcgtgc
     1021 ctaatacatg caagtagaac gctgaaggag gagcttgctt ctctggatga gttgcgaacg
     1081 ggtgagtaac gcgtaggtaa cctgcctggt agcgggggat aactattgga aacgatagct
     1141 aataccgcat aagagtagat gttgcatgac atttgcttaa aaggtgcact tgcatcacta
     1201 ccagatggac ctgcgttgta ttagctagtt ggtggggtaa cggctcacca aggcgacgat
     1261 acatagccga cctgagaggg tgatcggcca cactgggact gagacacggc ccagactcct
     1321 acgggaggca gcagtaggga atcttcggca atggacggaa gtctgaccga gcaacgccgc
     1381 gtgagtgaag aaggttttcg gatcgtaaag ctctgttgta agagaagaac gagtgtgaga
     1441 gtggaaagtt cacactgtga cggtatctta ccagaaaggg acggctaact acgtgccagc
     1501 agccgcggta atacgtaggt cccgagcgtt gtccggattt attgggcgta aagcgagcgc
     1561 aggcggttag ataagtctga agttaaaggc tgtggcttaa ccatagtagg ctttggaaac
     1621 tgtttaactt gagtgcaaga ggggagagtg gaattccatg tgtagcggtg aaatgcgtag
     1681 atatatggag gaacaccagt ggcgaaagcg gctctctggc ttgtaactga cgctgaggct
     1741 cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgctgt aaacgatgag
     1801 tgctaggtgt tagacccttt ccggggttta gtgccgtagc taacgcatta agcactccgc
     1861 ctggggagta cgaccgcaag gttgaaactc aaaggaattg acgggggccc gcacaagcgg
     1921 tggagcatgt ggtttaattc gaagcaacgc gaagaacctt accaggtctt gacatccctc
     1981 tgaccgctct agagatagag ctttccttcg ggacagaggt gacaggtggt gcatggttgt
     2041 cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac ccctattgtt
     2101 agttgccatc atttagttgg gcactctagc gagactgccg gtaataaacc ggaggaaggt
     2161 ggggatgacg tcaaatcatc atgcccctta tgacctgggc tacacacgtg ctacaatggc
     2221 tggtacaacg agtcgcaagc cggtgacggc aagctaatct cttaaagcca gtctcagttc
     2281 ggattgtagg ctgcaactcg cctacatgaa gtcggaatcg ctagtaatcg cggatcagca
     2341 cgccgcggtg aatacgttcc cgggccttgt acacaccgcc cgtcacacca cgagagtttg
     2401 taacacccga agtcggtgag gtaaccgtaa ggagccagcc gcctaaggtg ggatagatga
     2461 ttggggtgaa gtcgtaacaa ggtagccgta tcggaaggtg cggctggatc acctcctttc
     2521 taaggataag gaactgcgca ttggtcttgt ttagtcttga gaggtcttgt ggggccttag
     2581 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
     2641 ccattggtga gagatcacca agtaatgcac attgaaaatt gaatatctat atcaaatagt
     2701 aacaagaaaa taaaccgaaa acgctgtagt attaataaga gtttatgact gaaaggtcag
     2761 aaaataaggt taagttaata agggcgcacg gtggatgcct tggcactagg agccgaagaa
     2821 ggacgtgaca aacgacgata tgccttgggt agctgtaagt aagcgatgat ccagggattt
     2881 ccgaatgggg gaacccaaca ggtaatacct gttacccaca tctgttaagg atgtgaggag
     2941 gaagacgcag tgaactgaaa catctaagta gctgcaggaa gagaaagcaa aagcgattgc
     3001 cttagtagcg gcgagcgaaa cggcaggagg gcaaaccgaa gagtttactc ttcggggttg
     3061 taggactgca atgtggactc aaagattata gaagaatgat ttgggaagat cagccaaaga
     3121 gagtaatagc ctcgtattta aaatagtctt tgtacttagc agtatcctga gtacggcggg
     3181 acacgtgaaa tcccgtcgga atctgggagg accatctccc aaccctaaat actccctagt
     3241 gaccgatagt gaaccagtac cgtgagggaa aggtgaaaag caccccggga ggggagtgaa
     3301 atagaacctg aaaccgtgtg cctacaacaa gttcgagccc gttaatgggt gagagcgtgc
     3361 cttttgtaga atgaaccggc gagttacgtt atgatgcgag gttaagttga agagacggag
     3421 ccgtagggaa accgagtctg aatagggcgc cttagtatca tgacgtagac ccgaaaccat
     3481 gtgacctacc catgagcagg ttgaaggtgc ggtaagacgc actggaggac cgaaccaggg
     3541 cacgttgaaa agtgcttgga tgacttgtgg gtagcggaga aattccaaac gaacttggag
     3601 atagctggtt ctctccgaaa tagctttagg gctagcgtcg acattagaga ttcttggagg
     3661 tagagcactg tttgggtgag gggtccatcc cggattacca atctcagata aactccgaat
     3721 gccaatgaat tatggtcggc agtcagactg cgagtgctaa gatccgtagt cgaaagggaa
     3781 acagcccaga ccaccagcta aggtcccaaa ataattgtta agtggaaaag gatgtggggt
     3841 tgcacagaca actaggatgt tagcttagaa gcagctattc attcaaagag tgcgtaatag
     3901 ctcactagtc gagtgaccct gcgccgaaaa tgtaccgggg ctaaaacaat ttaccgaagc
     3961 tgtggatacc tttataggta tggtaggaga gcgttctatg tgtgatgaag gtataccgtg
     4021 aggagtactg gaacgcatag aagtgagaat gccggtatga gtagcgaaag acaggtgaga
     4081 atcctgtcca ccgtaagact aaggtttcca ggggaaggct cgtccgccct gggttagtcg
     4141 ggacctaagg agagaccgaa aggtgtatcc gatggacaac aggttgatat tcctgtacta
     4201 gagtatgtag tgatggaggg acgcagtagg ctaactaaag cagacgattg gaagagtctg
     4261 tctaagcagt gaggtgtgaa ttgagtcaaa tgcttaattc tataacattg agctgtgatg
     4321 gggagcgaag tttagtagcg aagttagtga cgtcacactg ccaagaaaag cttctagcgt
     4381 ttaaacatac tctacccgta ccgcaaaccg acacaggtag tcgaggcgag tagcctcagg
     4441 tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt cgggagaagg
     4501 ggtgctgact ttaagtcagc cgcagtgaat aggcccaagc aactgtttat caaaaacaca
     4561 gctctctgct aaatcgtaag atgatgtata gggggtgacg cctgcccggt gctggaaggt
     4621 taagaggagt gcttagcgta agcgaaggta tgaattgaag ccccagtaaa cggcggccgt
     4681 aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ccgcacgaaa
     4741 ggcgtaatga tttgggcact gtctcaacga gagactcggt gaaattttag tacctgtgaa
     4801 gatgcaggtt acccgcgaca ggacggaaag accccatgga gctttactgc agtttgatat
     4861 tgagtgtctg taccacatgt acaggatagg taggagtcta agagatcggg acaccagttt
     4921 cgaaggagac gctgttggga tactaccctt gtgttatggc cactctaacc cagataggtg
     4981 atccctatcg gagacagtgt ctgacgggca gtttgactgg ggcggtcgcc tcctaaaagg
     5041 taacggaggc gcccaaaggt tccctcagaa tggttggaaa tcattcgcag agtgtaaagg
     5101 tataagggag cttgactgcg agagctacaa ctcgagcagg gacgaaagtc gggcttagtg
     5161 atccggtggt tccgtatgga agggccatcg ctcaacggat aaaagctacc ctggggataa
     5221 caggcttatc tcccccaaga gttcacatcg acggggaggt ttggcacctc gatgtcggct
     5281 cgtcgcatcc tggggctgta gtcggtccca agggttgggc tgttcgccca ttaaagcggc
     5341 acgcgagctg ggttcagaac gtcgtgagac agttcggtcc ctatccgtcg cgggcgtagg
     5401 aaatttgaga ggatctgctc ctagtacgag aggaccagag tggacttacc gctggtgtac
     5461 cagttgtctt gccaaaggca tcgctgggta gctatgtagg gaagggataa acgctgaaag
     5521 catctaagtg tgaaacccac ctcaagatga gatttcccat gattatatat cagtaagagc
     5581 cctgagggat gatcaggtag ataggttaga agtggaagtg tggcgacaca tgtagcggac
     5641 taatactaat agctcgagga cttatccaaa gtaactgaga atatgaaagc gaacggtttt
     5701 cttgaattga atagatattc aattttgagt aggtattact cagagttaag tgacgatagc
     5761 ctaggagata cacctgtacc catgccgaac acagaagtta agccctagaa cgccggaagt
     5821 agttgggggt tgccccctgt g
//
LOCUS       DAVKDZ010000107         5648 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000107 DAVKDZ010000000
VERSION     DAVKDZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.108"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..366
                     /locus_tag="VWZ30_001976"
     CDS             <1..366
                     /locus_tag="VWZ30_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU9335640.1"
                     /translation="PNLEQVQTMLEQAFKEKHYENTILHSDQGWQYQHDSYHRFLESK
                     GIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKSFRSLENLEQAIVDYIDYYNNKR
                     IKVKLKGLSPVQYRTKSFE"
     gene            complement(710..862)
                     /gene="cibB"
                     /locus_tag="VWZ30_001977"
     CDS             complement(710..862)
                     /gene="cibB"
                     /locus_tag="VWZ30_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEU9335641.1"
                     /translation="MMKDLNNYCEISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(864..1049)
                     /gene="cibA"
                     /locus_tag="VWZ30_001978"
     CDS             complement(864..1049)
                     /gene="cibA"
                     /locus_tag="VWZ30_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEU9335642.1"
                     /translation="MINFDVLDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            1227..1910
                     /gene="tsaB"
                     /locus_tag="VWZ30_001979"
     CDS             1227..1910
                     /gene="tsaB"
                     /locus_tag="VWZ30_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU9335643.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLPFERVIELIKGASQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            1907..2344
                     /gene="rimI"
                     /locus_tag="VWZ30_001980"
     CDS             1907..2344
                     /gene="rimI"
                     /locus_tag="VWZ30_001980"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU9335644.1"
                     /translation="MIEIKRIQQQPALAQAIYAVMAAVYPVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLTVQETLFEAEVLQIAVKGACQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            2334..3344
                     /gene="tsaD"
                     /locus_tag="VWZ30_001981"
     CDS             2334..3344
                     /gene="tsaD"
                     /locus_tag="VWZ30_001981"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU9335645.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAEITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDMYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNSEQKGESLSAEDLCASFQAAVLDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLATEITDVNVMIPPLRLCGDNAGMIAYASVSEWNKENFASLDLNAKPSLTFDT
                     IE"
     gene            3448..4311
                     /locus_tag="VWZ30_001982"
     CDS             3448..4311
                     /locus_tag="VWZ30_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9335646.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACDGLTASQLSKFELGQSM
                     LSADKLILAIQGINMNFDEFGHKLNNYQESPHMRIGRKVVDRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKNALHSLDKSYPLTEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRWYMKSGYLNIISELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVLWQFLKKMQIFLQTKGSNQKEIEHFIQSLQVLENPQLTALFE
                     LRLQQYKELID"
     gene            4480..4706
                     /locus_tag="VWZ30_001983"
                     /pseudo
     CDS             4480..4706
                     /locus_tag="VWZ30_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4791..>5648
                     /locus_tag="VWZ30_001984"
     CDS             4791..>5648
                     /locus_tag="VWZ30_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU9335647.1"
                     /translation="MDRLQTMLNKIQVDTYHKNGWLFVKYSNNKLTQGWKLHVSSQLK
                     DACNIFYIVAQELEKERCNYKVLDCLDELKKLNSPREVSPTANKFITIYPSSRKQAKR
                     IILNLKEKLEKYKAPRILSDFQCGEYSPIHYRYGAFKAIKNYNFSENKIVYMMKNSRG
                     ELVEDLRLSYPYLPEGVKDLFTDMEKNKLFKPSPEIPENHPLNHYNIECILKKSNRGN
                     VYRAIEKSSNQKVIIRHARPYVYSWNDGFSAIDELKSEAANLKVFQDKSYTTDLIEEF
                     YVNEDYFVVQ"
BASE COUNT         1825 a         1019 c         1183 g         1621 t
ORIGIN      
        1 ccgaacttag aacaagtaca aacaatgttg gaacaggcat tcaaagagaa gcactatgag
       61 aatacgattc tccatagtga ccaaggctgg caataccaac acgattctta tcatcggttc
      121 ctagagagta agggaattca agcatccatg tcacgcaagg gcaacagccc agacaacggc
      181 atgatggaat ctttctttgg cattttgaaa tcggagatgt tttatggtta tgagaagagc
      241 tttagatctt tagaaaacct tgaacaagct attgtggact acattgatta ctacaacaac
      301 aagagaatta aggtaaaact aaaaggactt agtcctgtgc agtacagaac taaatccttt
      361 gaataaatta tttgtctaac tttttggggg cagtacatat gctggggctt tttgtttgag
      421 gatatagaga tagtagattt acgacacgaa aattccaccc catctatcaa gatgaggtga
      481 tctttgcatt tttctaaaat gacatgacaa tggaattttc agagcttatt atttttatgg
      541 gagtcattgt tctgctcaaa aatagagttg aagcataaaa ataaaacaat acaaacagat
      601 agatcaattg gtaaagggat agtagtcatg tttaaatcat tgagaatgct gagcaaactg
      661 taagagatga agacaataaa atttatcctt ctaaaaatgt gtttcttatc tagcatgact
      721 taccaaactt tttacgaagg tcttttccaa tggtataacc tgccataaat aaagcgatac
      781 caacacctac accaaagcca ccctttattt cttgcaattc cttattagaa atttcacaat
      841 agttgttcaa atctttcatc atattaatct cctcctaaaa aataaccagc tgccaaacca
      901 gaacctataa gtgctgttcc acctgcaata gctttccaag atactgctac tccaaagata
      961 accaagggag cgagaccgcc atcaatatct gataattcat tttcagataa ggataaaaat
     1021 tgattgtcaa gaacgtcaaa atttatcatt gaactttcct ccttttttct tgttcacttt
     1081 tatattcgta aaagaaaagg aaaagtgagc atttttttaa attattttta gttaagtgag
     1141 ggcactcttg tctgggggca aagaaaattc ccatgtgcca gcttttgtag tataatagta
     1201 agcagataaa aagataggaa tgtgttatga aagtattagc ttttgatacg tccagcaagg
     1261 ctctttctct ggctatttta gaggacaagc aggttcttgc cgagacgacg attaatatca
     1321 agaaaaatca cagcattacc cttatgcctg ccatcgattt tttgatggca agtttggatt
     1381 ggacacccaa ggatttagac cgaatcgtgg tggcggaagg accaggtagc tatacaggct
     1441 tgcgaattgc ggtagcaact gccaaaactc tggcttatac tctgaatatc gagttggttg
     1501 gtatgtcgag tctcttggct ctggtgcctt accaacaaga aggtttgttt gtccccttga
     1561 tggatgcgcg tcgcaataat gtttatgcag gattttatga aaatgccaaa cctgtcatgg
     1621 cagaagcaca tctgcctttt gagagagtca ttgagctaat caagggcgct agtcaggtaa
     1681 cctttgtcgg agaagttggc ccctttgttg agcagattca aaaacacttg ccaaggactg
     1741 attacaaaga aacattgccc aatgcagcta atctagctct tttggcctgg gacaaggaag
     1801 cagactcctt gcatgatttt gtgccaaatt acctcaaacg tgttgaggca gaggaaaact
     1861 ggctcaagaa ccataccgag tctggtgagt cctacattaa acgcctatga tagaaatcaa
     1921 acgaatccaa cagcaacctg ccttggctca agctatctac gctgtcatgg cagctgttta
     1981 cccagtcagt ccttggacac tagaacaaat ccaagcagat ctgtcccaag accagacttg
     2041 gtatgctcta gcttatgatg gggcagaagt gattggcttt ctaactgttc aggagactct
     2101 ctttgaagca gaagtcctgc aaatcgctgt caaaggagcc tgtcagggtc agggaattgc
     2161 gtcagcttta tttgctcaat tgccgacaga taaggagatt ttcctcgaag tcagaaagtc
     2221 aaatcaacga gcgcaagcat tttacaagaa agaaaagatg gcggtcatcg cggagcgaaa
     2281 agcctactac catgacccag tcgaggacgc cattatcatg aagagagaaa tagatgaagg
     2341 atagatatat tttagcattt gagacatcct gtgatgaaac cagtgtcgcc gtcttgaaaa
     2401 acgacgatga gctcttgtcc aatgtcattg ctagtcaaat tgagagtcac aaacgttttg
     2461 ggggcgtagt gccagaagta gccagtcgtc accatgtaga ggtcattaca gcctgtatcg
     2521 aggaggcgct agcagaagca gagattacag aagaggacgt gacagctgtg gcggttacct
     2581 atggaccggg cttggtcgga gccttactag ttggtttgtc agctgccaag gcctttgctt
     2641 gggctcatgg acttccgctg attcctgtga accacatggc gggtcatctc atggcagctc
     2701 agagcgtgga gcctttggag tttcccttgc tagccctatt ggtcagcggt ggacacacgg
     2761 agttggttta tgtttctgag gctggtgatt acaagattgt tggagagacc cgagatgatg
     2821 cggttgggga ggcctatgat aaggttggcc gcgttatggg cttgacctat ccagccggtc
     2881 gcgagattga cgagctagct catcaggggc aggatatgta tgacttccct cgtgctatga
     2941 tcaaggaaga caatctggag ttttcattct caggtttgaa gtctgccttt atcaatcttc
     3001 accacaattc cgagcaaaag ggagaaagtc tgtctgcaga ggacttgtgt gcttccttcc
     3061 aagcagctgt cttggatatt cttatggcaa aaactaagaa ggctttagag aaataccctg
     3121 ttaaaaccct tgtcgtggca ggtggcgtgg cagctaataa aggtctcaga gaacgcctag
     3181 caactgaaat cacagatgtc aatgttatga ttccacctct gcgtctctgc ggagacaatg
     3241 caggtatgat tgcctatgct agcgtcagcg agtggaacaa agaaaacttt gcaagtttgg
     3301 atctcaatgc caaaccaagc ctcacttttg ataccataga ataaagagtc agcttagggc
     3361 tggctgtttt atttttctaa ctatcagaat attttgacaa atgaacacaa ataatgatat
     3421 aatatgcaaa agctaggagg tggtaggatg attgaaaaaa tggaattggg agaattttac
     3481 aaggaactgc gcctggcgag aaagctcaag cagtcagatg tggcttgtga cgggctaaca
     3541 gcttctcagt tgtccaagtt tgagctaggg cagtccatgt tgtctgctga taagctcata
     3601 ttagccattc aagggatcaa tatgaatttt gatgagtttg ggcacaagct caacaactat
     3661 caagaatctc cacatatgcg aatcggtcga aaggttgtgg atcgctttgc ccatcaggat
     3721 atagctgctt tagagcagct attggaggaa gtcgatcaag aacagatggc gcagacctat
     3781 cgtcgtttga atgccattgt gattaaaaat gcactccatt ccttagataa aagctacccg
     3841 ctaacagagg aggatagcga gtttttgact acctacctct acgctattga gtcttggacc
     3901 tggtttgaac tctatctttt ttgcaatact atgcccttct tgagcaatca agatctgatc
     3961 tttttatcaa cctccttact cgaaaaatcc aaggaattta aagagttagt acacaatcga
     4021 tggtatatga aaagtggtta tcttaatatc atctcagaac ttatggagcg caagctattt
     4081 tcttatattc caatctttga agctgagctg gagcgcatgc tccgtccata cgatgttttt
     4141 gaaaaagtat tgtggcagtt tttaaagaaa atgcagattt ttcttcaaac caaaggaagc
     4201 aatcaaaaag agattgaaca ctttatccaa tctctgcaag tattagaaaa tccacaattg
     4261 acagcccttt ttgaattgcg tttgcagcaa tacaaagagc ttatcgatta gttagaaatg
     4321 ggaaaaagat aataatatca gcaacgatcg tcaataatgt taaaacttgt ttcttcatat
     4381 tacttccctc ctcattagta ataactttat tataccaggg aaataatcaa atctaccaaa
     4441 atcgcaaatc tgaaatttct ataagaaaaa atatcaaata tgcgattttc taaaataagc
     4501 caattttcgt gttataattt tcgtgttata ctgtacttgt aaagcacttg aagcaaatcc
     4561 taggtcgcag aagtggttta caaaaaaata atcaaaggag aaaagaaatg cataatctaa
     4621 tggaacttga attgttacct gaagagccag taattactgt atcacgggaa gtttgtaagt
     4681 ggacttgctc atttactgct ctttaagaat aactggttaa aattaaggat agttgattac
     4741 taattaacta tccttaattt catttcagag ataatgtatt tattatattt atggatagat
     4801 tacaaacaat gctgaataaa atacaagttg atacttacca taaaaatgga tggttatttg
     4861 tgaagtatag taacaataaa cttacacagg gatggaaact tcatgtaagc agtcaactaa
     4921 aagacgcttg taatattttt tatattgttg cacaagagct ggaaaaggaa agatgtaact
     4981 acaaagttct agattgtttg gatgagttaa aaaaattaaa ttctcctagg gaagtttctc
     5041 ctactgcaaa taagtttatc actatatatc cgtctagtag gaagcaggcc aaacgaataa
     5101 tattaaatct aaaagaaaag ttagaaaaat ataaagctcc aaggatttta tcagattttc
     5161 aatgtggtga gtattctcca atacattata gatatggtgc gtttaaagca attaaaaatt
     5221 acaatttttc tgaaaataaa attgtctaca tgatgaaaaa tagtagagga gaattagtag
     5281 aagatctgag attaagttat ccatatttgc cagaaggggt taaggattta tttactgata
     5341 tggaaaaaaa taaattattt aaaccctccc cggaaatccc agagaatcac cctttaaatc
     5401 actataatat cgagtgtatt ctaaaaaaat ccaatagagg aaatgtttac agagctatag
     5461 aaaaaagtag taatcagaaa gtgataataa gacacgcacg gccatatgtt tatagttgga
     5521 atgatggttt ctctgcaatt gatgaattaa agagtgaagc agcaaattta aaagtatttc
     5581 aggataagtc ctatacaaca gatttaattg aagagtttta tgtcaatgaa gattacttcg
     5641 tagtacag
//
LOCUS       DAVKDZ010000108         5643 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000108 DAVKDZ010000000
VERSION     DAVKDZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.109"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..1569
                     /locus_tag="VWZ30_001985"
     CDS             <1..1569
                     /locus_tag="VWZ30_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecF"
                     /protein_id="HEU9335648.1"
                     /translation="SKLFKFGKRLKSLLDVEIAKIDIKSIENSLNNFTGWEFGRLWIE
                     KFEPKLTEYKFKEGQLDDGTESINKLLEIRTRLKEHSTGNHSECIFCGYDWGESENLL
                     AAYEETTNQLRKNLTNFEIEVSNLVEELNQLKMELQNQIQMEQEKLFVIPDDFLAAIR
                     SISNYNFPESFSNLVVMNSEITPLPVDKSASLQEFENLKTELISSLQEKLLIPNEVYG
                     TFLRVVNKSVDFEKLLEKYSILAGESIREYEISFDLLPISLHKFEENKDRLLVFLGDL
                     RSQIVFDYSKSLDSQNLFDKYFASKEKTFEDCTIESINQKREYIINQFELQSLTMFHQ
                     IEKRLQIIEQTITYLQDRVRELNNNIKSYQEQMIQKLKLPFYMYTAKILQNYQQGMGV
                     LLTTQNNSNIRFIANSNSEQDVMYQLSSGQVAVISFAFTLALNTTFKISRGFKFLSID
                     DPIQDMDSMNVYALIDLIRHSLSDYQIIMSTHNDGSAMFIKYKFELFSDSEISNVGLK
                     NIKNILLLDVEVSS"
     gene            1958..2317
                     /locus_tag="VWZ30_001986"
     CDS             1958..2317
                     /locus_tag="VWZ30_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335649.1"
                     /translation="MGQEIKLIRKQFRITRQEEKQIKEMMREQKVDSFSEFLRQNLLK
                     KNYQDRIFESWFSLWQSQKFEQISRDVYEVLVVARENHQVTQEHVSILLTCVQELIAE
                     VNQVQPLSREFREKYMG"
     gene            2327..2692
                     /locus_tag="VWZ30_001987"
     CDS             2327..2692
                     /locus_tag="VWZ30_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEU9335650.1"
                     /translation="MVYRYRTNLKKVFLTDPELHQLNERISKSNCQNFSVYARKVLLS
                     PNMSFVTINTDTYDQLVFELRRIGNNINQIARAINQSHLISQDQLQELSKGVGELIKE
                     VDKEFQVEVKRLNEFHGSY"
     gene            2679..4545
                     /locus_tag="VWZ30_001988"
                     /pseudo
     CDS             2679..4545
                     /locus_tag="VWZ30_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261806.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4590..5444)
                     /locus_tag="VWZ30_001989"
     CDS             complement(4590..5444)
                     /locus_tag="VWZ30_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HEU9335651.1"
                     /translation="MKSKLGITLRKVRKGKQISLCSVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDGFLVLHNDDYTSSESFANLVQYIRKQYSSQSINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSNIIPSDEELLHLTDYLFKVEKWGYYEIILLVNCVRT
                     INYNSYFLLTKEMLNNYIYSSLNKTNKQIVSQLAINCFILSIGKEEFSNCSYLISKIK
                     TLLDNKLNFYEQPVFLYATGYFEFKRCQSTSGIEKMKQAIQVFDILGEDKLKLHYTDH
                     FNKLVNKK"
     gene            5524..5586
                     /locus_tag="VWZ30_001990"
     CDS             5524..5586
                     /locus_tag="VWZ30_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HEU9335652.1"
                     /translation="MKKISKFLPILVLVMDIIII"
BASE COUNT         2056 a          774 c         1014 g         1799 t
ORIGIN      
        1 agtaagttgt ttaaatttgg aaaaagattg aagagcttac ttgatgttga aattgctaaa
       61 attgatatca aatcaattga gaattcttta aataatttta caggatggga atttggcagg
      121 ctatggattg aaaaatttga acctaaatta acagaatata agtttaaaga agggcagcta
      181 gatgatggaa cagagtcgat taacaagcta ctcgagataa gaactcgtct aaaagaacat
      241 agcactggaa atcattcgga atgtatattt tgtggttatg attggggaga gagcgagaat
      301 ttactggcag cctatgaaga aacaacgaat caactacgta aaaatttgac taattttgaa
      361 atagaagttt caaatttggt tgaagaatta aatcaattaa aaatggagtt acaaaatcag
      421 atccaaatgg agcaagagaa actctttgtt attcctgatg attttttggc ggcgatacga
      481 tccataagta attataattt tcctgagtct tttagcaact tggttgtaat gaactcagag
      541 attactcctt tgcctgttga taagagtgct tcgctacaag agtttgagaa tttgaaaaca
      601 gagttaataa gttcattgca agaaaaacta ttgattccaa atgaagttta tggtactttc
      661 cttagagttg taaataagag tgttgatttt gaaaaacttt tagaaaaata ttctatttta
      721 gctggagaat caataaggga atatgaaatt tcttttgacc ttttacccat ttccttgcat
      781 aaatttgaag agaacaaaga caggttacta gtatttttag gggatcttag atctcaaata
      841 gtatttgatt attctaaaag tttagattca caaaatctat ttgataaata ctttgcatct
      901 aaggaaaaaa cttttgagga ctgtactata gaaagtatca atcaaaagag agaatatatt
      961 attaaccagt ttgaattaca aagtttaacg atgtttcacc aaattgagaa acgtcttcaa
     1021 atcattgaac aaacgattac atatcttcag gatcgtgtta gggaattaaa taataatata
     1081 aagtcttacc aagaacagat gattcaaaag ttgaagcttc ccttctatat gtacactgct
     1141 aaaattttac aaaattacca acaaggaatg ggagttctac taacaactca aaacaattct
     1201 aatatcaggt ttatagcaaa tagtaatagt gagcaggatg tgatgtatca attgagttct
     1261 ggacaagtag ctgttatttc gtttgctttc actttggcac taaatactac atttaagatt
     1321 tcaagagggt tcaaattttt atcaattgat gatccgattc aagatatgga ttctatgaat
     1381 gtatatgctt taattgattt aataagacat tcattatcag actatcaaat tattatgtct
     1441 acacataatg atggtagtgc tatgtttata aaatataaat ttgaactatt ttcagattcc
     1501 gaaatatcaa atgtagggct aaaaaatata aagaatatct tattattgga tgttgaagtt
     1561 agcagttgat gagactatat atattaatgg aaatataaaa aacatggttt cattaagacg
     1621 gtatttgaga gccttggctt tcttttttct taccatcatt tttcatgttg tgatactttt
     1681 ttcaaattta ccttgctatc ctaatatcat tactaagaaa atcaagcctt aatttttcac
     1741 tgggggtttg ggggcggagc caccaagtta tcttatcgtt ggctgtcaaa actagaaggt
     1801 tttgacaggc tggcgatatg atttttggga tattgtggac acaatatctg agctcgcaaa
     1861 gaccgaacaa ggagagactg aggtctttag gaatcgtact gacaatgtga ggcagcctta
     1921 cattgtcaga caagtataat gttgaaagga tggtaagatg ggacaagaaa ttaagttaat
     1981 ccgtaagcaa tttagaatca cgagacaaga agaaaaacag ataaaagaaa tgatgaggga
     2041 acaaaaagtg gatagtttct cagaatttct tcgtcaaaat ttattgaaaa agaattatca
     2101 ggatagaatt tttgaaagtt ggttctccct ttggcagtct caaaagtttg aacagattag
     2161 tcgagatgtg tatgaagttc tggttgtcgc aagagaaaat catcaagtga ctcaagagca
     2221 cgtctcaatc ttattgactt gcgttcaaga attgattgca gaagtgaatc aagtacagcc
     2281 actcagtcgt gagtttcgtg aaaaatatat gggttaggag gatgtaatgg tttatcgcta
     2341 tcgtaccaat ctcaaaaaag tatttctaac tgatccagaa ttacaccaat tgaatgaacg
     2401 gatttctaag agcaattgtc aaaatttttc agtctatgcc agaaaagttt tgcttagtcc
     2461 caatatgtca tttgtcacga ttaacactga tacttatgac cagttagttt ttgaattgag
     2521 acgtattgga aataacatca atcaaattgc gcgtgcgatt aatcaaagcc atctgatttc
     2581 tcaggaccaa ttacaagaat tgagtaaagg agtcggagaa ttaattaagg aagtggacaa
     2641 agaatttcaa gtggaggtga aaagactgaa tgagtttcat ggtagttact aaacactttg
     2701 cgacgcacgg aaaaaaaata tcgtaggcgt ttgattaaat atatcctcaa tccagataaa
     2761 acagataatc taaaattagt atctgatttt ggcatgagta attacttaga ttttcctagc
     2821 catgcagaaa tggtagaaat gtacaatgtt aactttacta ataacgacaa gttgtacgag
     2881 tccagaaatg accgacaaga aaaacactaa caaactattc atgcccatca tctcatccaa
     2941 tcattttctc ctgaagataa tctgacacct gaagaaatta accgcattgg ttatgagacc
     3001 atgatggaat tgacaggagg tcgttttaag tttatcgtag caactcacac agacaaagat
     3061 catgttcata atcacatcct aatcaacgct attgaccgca attcagataa aaagttgata
     3121 tggaactatg ccttggaacg aaacctccgt atgatttcag accgtatttc taaaatggct
     3181 ggtgcaaaaa ttattgagaa acgttattcc tatctagatt ataaaaaata taaggaatct
     3241 agtcataaat ttgaattgaa acaacgtctt tattttttgc ttcagcaatc taaatcgttt
     3301 gatgattttt tagagaaagc aaagcagttg catgttcaga ttgattttag tcaaaagcat
     3361 agccgttttt tgatgacaga tagaacgatg gtaaaaccaa ttcgaggtcg ccaacttagt
     3421 aaacgagatt tatatgatga agactttttt aaaacatatt ttgccaaaca agagattgaa
     3481 agtcgtttaa aatttttact aaagtctgtt tattcattag aagaattgca tgtaaaagct
     3541 aaagagttga atctaaccat cgagttaaaa cagaaaaatg tgatgtttac cctagaagaa
     3601 gatggtaaaa agataagcct gagtcataaa aaaattagcg ataagaaatt gtacgatgtt
     3661 caatttttta atcgttattt tgaagataga gaagttagag atattcaagt attagaaaat
     3721 cttcaggaag attttcagac ttttcgagag gagcagcata aggagaaagt ctccgctgaa
     3781 gaaatcgagg aagccttcaa gaaatacaag gaaaaacgag atgctatcca tgagtttgag
     3841 attgaactta cggataatca gatagagaag ttggttgatg atggtgtcta tatccaggtt
     3901 tcgtttggta tcaaacagag tggactcatc tttattctta actaccagct agatattttt
     3961 gaggaagaca atcataaaaa atataaagtc tacattcgag aaacgtcttc ttactttgtc
     4021 tataataaag aaaacatgga taataattgt ttcatcaagg gtaggacctt aatcagacaa
     4081 ttgagcaacg atagccaaaa actcccctat agacgaccaa ctttaaaaag tcttcaagag
     4141 aagatttctg aaattaatct tatgattgaa ctatctaaca cgaacaaaca ataccaagaa
     4201 atcaaagatg agcttgtatt agaaattgca gagattgata tgcagctaga agaaacccaa
     4261 gaaaaaatcg ccaccttaaa caagatggcg gaagtactta tcaatttgaa gagtgaagat
     4321 catgagacta gaaaattagc taagtatgat tttgaccaaa tgaacatgac ggaatcaata
     4381 atgttagatc gactcaatac agatatttta aaattacaac aggaacttgg taatgaaatt
     4441 aataaatatg aggagatagc tagaagattg gatttgtttg tgaaaattat taataccaat
     4501 aaatttacag ttctaaaatt tcacgagaat gccttactag aataatatta tttacgacta
     4561 attagttaaa agatagagaa taatttcatt tattttttgt ttactagttt attaaaatgg
     4621 tcagtatagt gtaattttaa cttatcttct cctaaaatat cgaagacttg tatggcttgt
     4681 ttcatttttt caattccact ggttgattgg caacgtttaa attcaaagta accagttgca
     4741 tagagaaaaa ctggttgttc ataaaagttt agtttattat ccaacaatgt ctttatctta
     4801 ctaattaaat aggagcaatt agaaaattct tccttaccaa tgcttagaat aaagcaatta
     4861 atagcaagct gagagacaat ctgcttatta gttttattca gagatgagta aatatagtta
     4921 tttaacatct ctttagttaa taagaagtag gagttgtagt taattgtgcg tacacagtta
     4981 actaataaaa tgatttcata atagccccat ttttcaactt tgaaaagata gtctgtcaga
     5041 tgaagaagct cttcatctga tggaatgatg ttcgaatcca ttgtgtgtaa aatagatttt
     5101 accattgttt tctcaaaaga atttagagtg taatcagaag tatcagaaag tagacatgct
     5161 atattattga tactttgtga tgaatattgc ttacgaatgt actgtactag attagcaaat
     5221 gattcggaac tggtatagtc gtcattgtgt aaaacaagga acccatccaa agtaatatgt
     5281 aatttgtcta aaatattgat tagtcgaata caagatatct ctgactcccc acgttcaaaa
     5341 cgagatattt gtgattttga aagatgttca tctgctacag agcatagact aatttgtttt
     5401 cctttacgaa cttttctaag tgtgatgcca agttttgatt tcataatttc ccattttccc
     5461 aacaaaaatt aaatccgttt ttatttattg tacaataaat aaaaacggaa aggaagtcca
     5521 agtatgaaga aaatttcaaa atttttaccg attttggttt tggtgatgga tattatcatc
     5581 atttaaaaaa gtgtattaat tattgtattt cacaattaga taatataaca gtaacaggtt
     5641 tag
//
LOCUS       DAVKDZ010000109         5625 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000109 DAVKDZ010000000
VERSION     DAVKDZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.110"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            455..1804
                     /locus_tag="VWZ30_001991"
     CDS             455..1804
                     /locus_tag="VWZ30_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335653.1"
                     /translation="MNTLPQKQFKRMKREVEEETARGIGYFEGILEHIPSYPLSEAVK
                     ELALSGWITPHTDVIDVQNMIVTESIKWMNYQDFKEVAPYLFSYPREQREKDLLVQPV
                     KISREYFEELKANAEELFHLKQDLLQVNNQLDAKIQELEMDQLPNGDQVIGVDLKAEE
                     VLLLRTPENAQVEEWEVRKDGLITDYRSNLSEYAQIVDALLEMENPEIDTILYEEVIN
                     YSKKTWPDSEPIQLPENIKELLNQEGKLDPSYYQIAQFETVKQAYIYGMISPSFQERF
                     PHYSDFIRNYVEDREQYEFFNYRTEAVSLAQEILVPRLGDINQILGTNNQELIVQEVV
                     GYSQGESWELAYLRDTKTEDRESVRFYLESEIGAWYKGSLTELAVIRFEDIDLEKGFN
                     GHQEAVYHIDENLLVQDKLKQAQLHFPDLKHFVEAEELEREQTQDIVAEKEEPEFSL"
     gene            1816..3078
                     /locus_tag="VWZ30_001992"
                     /pseudo
     CDS             1816..3078
                     /locus_tag="VWZ30_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
     gene            3082..3943
                     /locus_tag="VWZ30_001993"
                     /pseudo
     CDS             3082..3943
                     /locus_tag="VWZ30_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730738.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toprim"
     gene            5246..5554
                     /locus_tag="VWZ30_001994"
     CDS             5246..5554
                     /locus_tag="VWZ30_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335654.1"
                     /translation="MIEHLKQETFDLLMEIFFEDEATDSPKVNEVNQHISRKECLYIL
                     RRDMRIKTNYELEEVEMYPIVLKEIEGMSDERFEQLRDEILKMEMVDTMELILEDLKV
                     "
BASE COUNT         2019 a          854 c         1282 g         1470 t
ORIGIN      
        1 agttataaga gagaagaaat aaaaaatgcc atttggttta ttattgccat agtcatagtg
       61 gttaatttct ttttaattat ttcaatatca agagctcttt tgagtccaat catcatagca
      121 actttaatag tgtgtgcggt aggagcaaca ttgggatctc ttgcaatttc taatacctaa
      181 aagtacaagg cagccgtaat ggctgttttt ttgatgaaag gagaatcaaa tggatttaga
      241 acaattgaag caacgacgag atgagctgat taaagataat tgagtgggcg gatggcttaa
      301 ttaaagagaa ggaagaaaaa ctatggtaag tgaaagtgag aacgattgca gcttctgagc
      361 tagcgagatc ggcattggaa tctgctttac gttctgcagg tatcgtagat accttttacg
      421 gaccagataa tagaaagaaa ggaaatgaaa tagaatgaat acactaccac aaaaacaatt
      481 taaacgaatg aagcgtgaag ttgaggaaga aactgctcga gggattggtt attttgaggg
      541 gattttagaa catatccctt cttaccccct atctgaagcc gtaaaagagt tggctttatc
      601 tggctggatc actcctcata cagatgtcat cgatgtccag aatatgattg ttacagaatc
      661 tataaaatgg atgaattacc aggacttcaa agaagttgct ccctacttgt tttcttaccc
      721 tcgagagcaa cgagagaaag atttattggt tcaaccagta aaaatctcca gagagtactt
      781 tgaggaatta aaggccaatg cagaagaatt atttcacttg aaacaagatt tgcttcaggt
      841 taataatcaa ttagatgcta agatccagga attggagatg gatcagctcc ccaatggtga
      901 tcaagttatt ggagtggatt taaaagctga ggaggtattg ttgctacgaa ctccagaaaa
      961 tgctcaggtt gaggagtggg aggttcgtaa agatggcttg attactgatt atcgttccaa
     1021 tctttcagaa tatgctcaaa ttgtagatgc tttacttgaa atggagaatc cagagattga
     1081 tactattctc tatgaagaag taatcaacta ttctaagaaa acttggccag attcagaacc
     1141 aattcaactt ccagaaaata tcaaggaact attgaatcaa gagggcaagc ttgatccctc
     1201 atattatcaa atagctcagt ttgaaacagt aaaacaagct tatatttatg ggatgatttc
     1261 acctagtttt caggagcgtt tccctcatta ttcagatttc attaggaact atgtagaaga
     1321 tagagaacaa tatgagtttt tcaactatcg tacagaagcc gtctcattag ctcaggaaat
     1381 tctagtacca cgtttaggag atattaacca gattttagga acgaataacc aggagttaat
     1441 cgttcaagag gtcgttggtt attctcaagg tgaaagctgg gaactagctt atttacggga
     1501 tacaaagacg gaagatagag aatccgttcg attctatctt gaaagtgaga ttggagcttg
     1561 gtataaggga agtttgacag agttggctgt tatccgcttt gaggatattg atttagagaa
     1621 aggatttaat ggccatcaag aagcagtcta tcatatcgat gagaacttgt tagttcagga
     1681 taagctaaaa caagcccaac tccattttcc tgatttaaaa cattttgtag aagcggaaga
     1741 acttgagaga gaacagacac aagatatagt agcagaaaaa gaagagccag aattttcatt
     1801 gtagggggat aacagatggt gttaagcaaa gaagaagcca aaagattatc tattatttct
     1861 gttgcagagc aactaggcat ggagttaaag cgtacaggta gctatagcta taactgggta
     1921 gaacatgatt cctttgtaat agatgcaaag aaaaatgaat tttactggaa ctcaagaact
     1981 gaatttggag atgtcattca gttagttcaa accatacgag gtgtttctta caaggaggcg
     2041 atgaattttt tagaaactgg agaatttaag gcagtagatg tagaagctca gacggcctca
     2101 aaagagcctt ttagttatca cttggaaaga tatgagcgtc aggatttcag cgcctctaga
     2161 cgctatttga aggcacagag aggcctttca gatgatacga ttaacttctt tattagccaa
     2221 ggcagtatag cggaagcaat ccgaaaaaaa ggagactatt ttgagcctgt gattgtcttt
     2281 aaatataaag acaacacaag ctttttagct ggagcgagtc ttcagggaat agtagagaat
     2341 cgagagcact atcctgaacg tgggcgttta aaacaaatta tgagaaattc agacggacaa
     2401 ctaggatttt caattgatat cggaacacca aaacgtttga tctttgcgga agctccaatc
     2461 gatctaatga gctactatga gcttcataaa gatagtcttc aggatgtccg cttagtagct
     2521 atggatggag taaaagaagg aatcattagt agacggttta tggaattgta tgcagagata
     2581 aatgataaat cctatcaagt taatcagaat actgggaaag ctctagaagt agtcgctaga
     2641 acaacgaact attttaaaga tggtcagaat caagatatga ttaccttggc tgtagataat
     2701 gatatggctg gctataaatt tataactagt ctgcaggaaa aggggattcc agtacagatt
     2761 gcgattccgc ctatcgttca gagatatcag gagaaggaag attggaatga ctttctgaag
     2821 ggagggaata aatttcctaa ggagttagca cacgtttata cagtagacga aaagtcttgg
     2881 tattaccagg gcttttttga gaaggaacta gctcttgcaa aagcgcaaga gctaactgca
     2941 gatgatgtaa aagcctttgt gtcagatagg caattgacaa aggaagaagt ccgtcaagaa
     3001 tatacaagga ttgtcgatca agagaaagaa aggaaacata ctatgtcgga agtacatgaa
     3061 gcaggggctg agtatgaagt agaaaagcca gataatagtc gcttagcgca agctagacga
     3121 aagctggagc gactcaaagg agaagctgat gaggcaatta atgcggccta ttcccaccaa
     3181 gcactaacaa atgggcaacc catgaatgat aagcgaggaa gtgctagctt tatgcgtaag
     3241 caagaacaaa tagaaggtca agtcttctca aagctggatg agattaggca acaggaagag
     3301 cgagtagagc gcctggagca ccaacaacat ttgaaagaaa tggggttgaa tcgtcaggga
     3361 agtggtctgg atatgagtgt tcaaaatata cctcgaatcc gtgaagagtt agaaaaagct
     3421 aaacgtgggg agtcgttctt taccaaagct actctcaaac gctatcaaga agagttgact
     3481 agagttgagg ccatatctga acaaatgggt aaaacgagca ttcaaccagc aactcaagcc
     3541 ttaattgatg aggggctagt gaaccagtgg caaaaacaac caaatacgta ttttgtaaaa
     3601 ggattacgaa gagttgccct ggagttgact gaagaaggag agtttcaatt atcttcacaa
     3661 atcaagtatc atccgaaaac agatgaggaa agactgaagg tggatgaatt acttgccaaa
     3721 caaaggcaag aaaatgtagg attaacgccc tcgaatcagg aaaaatcaat ctcaccacaa
     3781 ccagagccta ttgaaaaaaa tcaaggcgaa gcagggtggt tagaaaaaaa ttgggacaat
     3841 ctcacgtttt cgatagaaaa taaaaaaaac agtcgtcatt gatccgacta gtatagatga
     3901 gatggttgaa aaattacaag tcaaagattc gcaagagaca ctagataatc gatacgaaaa
     3961 aatttatcag tataaccgaa aagatgagag ggatcgtgat ttggatggtg atggtatttc
     4021 caattctgat gagatgttgc agggaacaga tccatacaac ggtgcctcta accttcataa
     4081 aaaacaggaa gatagagaaa gaaggacaga tgttgaagcg gaaactggag ctattataac
     4141 cgatgcaatt gctactaaaa gttcagaaca aaccatttcc cagttagtcg caaataaaga
     4201 tacaaaagct ttagctgaac acttgaaaga aggaatgaag aactacttgg attcagaaca
     4261 gtttaaatca ttcttggata cgatgagtaa gtttcacaac tactcactca ataatattca
     4321 tttgctgaag atgcaaaatc ctaatgtttc ccaagttgct agtttcaata aatggaaaac
     4381 agattttgac agaacagtta aaaagggttc aaaagctctg aaaatctggg ttccgtatca
     4441 agtgaaaact aagatttttg ctaatcaaaa tgagttgagt ttttctccat ctgagaatga
     4501 aacggaagag aaggaagtca cagttactcg attcaagtta gggaatgtct ttgatgtatc
     4561 tcaaacagaa ggaaaagaat tgccaaaagc aattaatgaa ttgactggag atgttaaaga
     4621 ttatgaagat ttgtaccgag cagccaaagc tgtttcaatg gataatcagg ttcctatcag
     4681 ttttgaagaa attaaaagag agagcacaaa tggttattac tctccagatg aaaatcgaat
     4741 tgttatttca aaaggcttaa agggtcaaga acagatttta aaaaccattt ttcatgaaat
     4801 ggctcatgca gatttacata gaggaagcaa tgcccaatat ggggacgacc aatatcgtaa
     4861 gcaagaatta caagctgaaa gtgtcgctta tgtcgtggct aatcattttg gttttgatac
     4921 gagtagctac agttttggct atttggctat ttgggcaaaa gataaaaatg gttttgaaga
     4981 tatggtagaa caactgcagg ttgttcagaa ggaagcaaaa agtttgattg atcggatgga
     5041 tgctaagtta gaattagtga aaaataaaac tgtgacgaaa gataagtttg ctgataaact
     5101 ccaacaggca aaagaaaaat ctgagcagtt ggggaatcaa aaggcggagg ctgtaaagca
     5161 ggtggaagaa aaaaagtccc tgagcaaccc tcgataatgg aggcattagt ccgtaggaag
     5221 aaaaacaaaa aaggagaaac ggaagatgat tgaacatcta aaacaagaaa cttttgatct
     5281 tttaatggag atattttttg aggatgaggc tacagattcg ccaaaagtga atgaagtcaa
     5341 tcagcacatt tcacgtaagg aatgtcttta cattcttcgt agagatatgc gaattaaaac
     5401 aaactatgag ctagaagaag tagaaatgta tcccattgta cttaaagaaa ttgaagggat
     5461 gagtgatgaa cgatttgaac aactaagaga cgaaattctt aaaatggaga tggttgatac
     5521 aatggagctt atactggaag acttaaaagt ttagtcaaac ttttccctcg tgataggata
     5581 acattcctgc ttgcagggat taggggattg gggatttccc caaag
//
LOCUS       DAVKDZ010000110         5566 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000110 DAVKDZ010000000
VERSION     DAVKDZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.111"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            79..276
                     /locus_tag="VWZ30_001995"
     CDS             79..276
                     /locus_tag="VWZ30_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9335655.1"
                     /translation="MAGKITKKGSSMGNIANVFAGLIGASAGQSLLGSWGPSIAGMAL
                     LPFIVGAAIMITVVSFFTGRK"
     gene            360..926
                     /gene="amaP"
                     /locus_tag="VWZ30_001996"
     CDS             360..926
                     /gene="amaP"
                     /locus_tag="VWZ30_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEU9335656.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIQLLSWRQNS
                     AVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVLDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIVDRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            938..1108
                     /locus_tag="VWZ30_001997"
     CDS             938..1108
                     /locus_tag="VWZ30_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEU9335657.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1139..1747
                     /locus_tag="VWZ30_001998"
     CDS             1139..1747
                     /locus_tag="VWZ30_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9335658.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEVVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1778..1981
                     /locus_tag="VWZ30_001999"
     CDS             1778..1981
                     /locus_tag="VWZ30_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9335659.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGVAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2091..2363)
                     /locus_tag="VWZ30_002000"
                     /pseudo
     CDS             complement(2091..2363)
                     /locus_tag="VWZ30_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2858)
                     /locus_tag="VWZ30_002001"
     CDS             complement(2403..2858)
                     /locus_tag="VWZ30_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9335660.1"
                     /translation="MTIRFEEKVSTENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALQDLEGVFSIFDEQEFVELIHKIRLEDSNLHIGRFFINPQKQGQGLGSQALRKFVSL
                     AFENEDIDSISLNVLEANQRAQNLYQKEGFEIVQMVEAPVRKYIMKKGR"
     gene            2928..3587
                     /locus_tag="VWZ30_002002"
     CDS             2928..3587
                     /locus_tag="VWZ30_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU9335661.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNHFRCRYCKVRYPVWYALFELGLGLIFLLYSWGWLSLGQVILITAG
                     LTLGIYDFRHQEYPLLVWMTFHLILIAYSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASAMGILSFILQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            complement(3639..4790)
                     /locus_tag="VWZ30_002003"
     CDS             complement(3639..4790)
                     /locus_tag="VWZ30_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU9335662.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(4805..5359)
                     /locus_tag="VWZ30_002004"
     CDS             complement(4805..5359)
                     /locus_tag="VWZ30_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335663.1"
                     /translation="MFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFPL
                     VQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNSN
                     IEKFMILTYEADSENNIIYVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDIP
                     NFSELSETEDFEIIPRIEKQEGQK"
BASE COUNT         1707 a         1016 c          937 g         1906 t
ORIGIN      
        1 ttgatttttt gatattaaaa taaaaaagga gggtaggcaa tgttgtgatt aattattgct
       61 ggaggtctta ttggtctctt ggcaggtaaa atcactaaaa aaggtagttc tatgggaaac
      121 atcgcaaatg tattcgctgg tttaatcggg gcatctgcag gacaatctct tttaggtagt
      181 tgggggccat caatcgctgg aatggctttg ctcccattta ttgtaggtgc agcgattatg
      241 attactgtag tgtcattctt tacaggtaga aagtgaactt ttcgccagta aagttagcaa
      301 actattttta aatcaatgac gggaaaaata gtttaaatgt taaatcgaaa ggattgtata
      361 tgtcaaaagc aaagaaaata tgtttcatta ttttctgtat tttaatcttg acaattttcc
      421 ttcctgtttt gatagattat catcaagtta gtgatctagg tattcaacta cttagctgga
      481 gacagaactc cgcagttgaa ttctatcttg ctagatatgt cttttggggg acagtggttc
      541 tatcaacttt agttttatta tccattttag ttgtgatgtt ttatcctaaa cgttacttgg
      601 aaatccaact tgaaactaaa aacgatacat taaaattaaa gaattcggca atcgaaggtt
      661 ttgttagaag tttggttctt gatcatagat tgatcaagaa cccaactgtt catgtaaatt
      721 tacgaaaaaa taaatgtttc gttcatgtag aaggtaaaat tcttccttca gacaacatcg
      781 ttgacagatg ccaaataatt caaaatgaaa taactaatgg attgaagcag ttttttggta
      841 ttgagcgtca agtaaaactt gaagttgcag taaaaaatta ccaaccaaaa cctcaaaaca
      901 aaaagactgt tagtcgtgtg aagtaaggaa gtaaaaaatg gaatggctta aacaatatcg
      961 atatccaatt atcgctggtc tcataggcgt atttcttgct tgtttgattg tctcctttgg
     1021 cttcttcaaa acaatatttg tattgatttt aggagcactg ggagttgcag ctggattata
     1081 tatcgaaaaa aactatatag ataaataaaa attactaatt taattaaagg agtttcatat
     1141 gtcaaacgaa aaaaacacaa acactaacgt agaaaagaaa gatgctactg ttgtagctca
     1201 cgaaatcaaa ggggaactta cttacgaaga taaagttatc caaaaaatca ttggtctttc
     1261 actagaaaac gtttcaggtc ttttgggaat cgatggtggt ttcttctcaa atcttaaaga
     1321 aaaaatcgtt aacagcgatg acgtaacaag tggtgttaac gtagaagttg gtaaaacaca
     1381 agttgcagtt gacttaaacg ttattgttga gtaccaaaaa aatgttccag ctttatattc
     1441 agaaatcaga gaaatcgtat cttcagaagt tgctaaaatg actgacttgg aagttgttga
     1501 aatcaacgta aacgttgtcg acatcaaaac taaagaacag catgaagcag attcagtaag
     1561 ccttcaagat cgcgtatctg acgttgctga atcaacagga gaattcactt cagaacaatt
     1621 cgaaaaagct aaatctggtc ttggatctgg tttctcaact gttcaagaaa aagttagcga
     1681 aggtgtagaa gctgttaaag gtgcagcaaa tggtgtagta tctcacgaaa acactcgtgt
     1741 aaactaagat aaaataaata taacaggaga aattatcatg tcagtagaag aaaaattaaa
     1801 tcaagctaaa ggttctatta aagaaggtgt tgggaaagcc atcggtgatg aaaaaatgga
     1861 aaaagaagga gtagctgaaa aagttgtttc taaagtaaaa gaagtcgccg aagatgccaa
     1921 agacgctgta gaaggtgctg tagaaggtgt taaaaacatg ttgagtggcg acgataaata
     1981 aggttaaaag ttactttatc tttttagtaa cattagtcaa aagagtctga gtcaagatga
     2041 ttctcagaaa acaaaaagct agagattccc aattgcggaa ctctagcttt ttaattttgc
     2101 ctctttctct tattatattt cagcaggttg ttggccatga gtacgaatcc catgtcaatt
     2161 ctcacttgac gcttccctct cagatgacat ctcttataac ccaaacaaac ctttatctgc
     2221 ccaaagacag atttcatatc aatcttacgt ttagcgaaaa tttgtctacc cttggaagat
     2281 aaaagtgcct gatattcttt agtttttaaa cactggtaac gttcattcat atacagtccc
     2341 ttttgagggg ctgattcagg ttcataatcg cagtcaacat tgatttcaag gctgtttgct
     2401 ttctatctcc cctttttcat gatatatttt cgtacaggtg cttcaaccat ttgaacgatt
     2461 tcaaatcctt ctttttggta aagattctga gctctttgat ttgcctctaa gacatttaga
     2521 gaaatactat ctatatcttc attttcaaag gccaaactaa caaatttcct taaagcctga
     2581 ctacctaagc cttgcccctg tttctggggg ttgataaaaa atctcccgat atgaagattg
     2641 ctgtcttcta gcctgatttt atggataagt tccacaaact cttgttcatc aaagattgaa
     2701 aagactcctt ccaagtcttg caaggcttga agggtcagta gaaaaggaat ccttggtccc
     2761 atccattgtt cttgaaagga tttgcctagg gagttggacc actggcatac gaattgagcg
     2821 ttttctgtgc tcaccttttc ttcaaaacga attgtcatct tttcctcacc atcttatcta
     2881 tgtttctcca ttatactatt tctcccattt tttacgaata gataagtatg attgattttt
     2941 atttttttct cgtcgggagc attctcgcct cctttcttgg tttggtcatt gaccgttttc
     3001 cagagcaatc cattatcagt tcagccagtc actgcgattc ctgtcagact cgcttgcgtc
     3061 ccttagattt gattccgatt ctatcgcagg tttttaatca ttttcgctgt cgctactgca
     3121 aggtccgcta tcccgtctgg tatgccctct ttgaactagg cttaggactc atctttctgc
     3181 tttactcttg gggatggctc tccttgggac aagtcatcct aatcactgct ggtttgacct
     3241 tgggtatcta cgactttcgc catcaggaat atcccttact ggtctggatg actttccacc
     3301 taatcctcat agcttactct ggctggaatc tggtcatggt ctccttcctt gctcttggaa
     3361 ttttggctca ttttatcgat atccgcatgg gcgcagggga tttcctcttt ttagcttctt
     3421 gtgctctcgt ctttagcgta acggagttac taatcttgat tcagtttgct tctgcgatgg
     3481 gtatcctttc ctttatcctg caaaagaaaa aggaaagact tcctttcgtg cctttcctct
     3541 tacttgctac ttgtttgatt atttttggta agctactgct tgtttgataa agtccaattt
     3601 ctgccatatg tccttcatga aattatttca cagttaaatt ataaattatt tcttttgtac
     3661 aaagggatga tgttatcaaa tcgatctgtt cttctatctt cttgatactg atcaaaaaat
     3721 ttcatttcga ctgaaaatat ttcgcttata aactgtaaac gaatactttg tttagacatt
     3781 atagtcgcta gactgactag atgattactc aaaacgacgt ccagaatact ctttactttg
     3841 cttggttttt taacaaaaat ttgatcatcc aaaggttcaa tcattttgta acctttttgc
     3901 gcaatttgac gataaaagta agaatgttgc tttggagtca ataatcctaa cttaaaagct
     3961 cgatactcta aagcctgtat cgaaacattc aaatccgact tcaataaaat ataactatca
     4021 ggattgctga cacgcttgcc aaccctctct tcaaatttga ctaaaaactc ttcttttggc
     4081 aataaaaaac atgatgcaaa ataatttgct tcttgctcca aacgatcgcc atcttcattc
     4141 atatctttat atttatgtaa aagaatatgt cctagctcat gagctaagtc aaaatttcga
     4201 cgtacagatg atttattcgt tcccaacaca atataaggtc ttcccaattt tgaccatgcg
     4261 ctataagcat cagcttggcc attaattaat cgttccacga tatagatgcc tgaacgttct
     4321 aatttataaa gcaaatcatg attatctttt gaaataccta atttttctct ggcataaaga
     4381 gccaattcct caatggattc tcccttatga taagattcac tcactacatt acttagatca
     4441 tgaattgtaa tattaggtat aattacaaaa ctttcaaaat aatcaatcaa actatctacc
     4501 ttatgtaaat acatagtttg aatatctatt gttttccgtg ttgctaggtc tgcatttcta
     4561 aaggcaatta cagaagaatc aaatcgaatg ctctcttctt cctgttcaaa ataagttaaa
     4621 tcaacatgaa attggttggc caaatgcatt ttggttgata atttaggttt cgtttcgttg
     4681 gactcaaact gccaaatggc ttgttccgtt aaattaattc tctgagccaa ttctgctcta
     4741 cttaaaccat ttaacagccg taattctttc aatacccgac cattaaacat ttacacactc
     4801 cttactactt ttgaccttct tgtttttcta ttcttggaat aatttcaaaa tcttctgttt
     4861 ccgataattc tgaaaaatta ggaatatctt gatatttagc ttcttcgaaa tggtacgaac
     4921 ttgattgaat atactcagac aaatcttgaa tcaagtgtaa ctgaccagtt cgtgcatcag
     4981 gcataacaac atctacatat ataatattgt tttctgagtc cgcctcataa gttaaaatca
     5041 taaatttttc gatattcgaa tttttggtag cttgttcaat ttcttgaatc atttcatcag
     5101 aaactaactc catctgaatt ggaaaggaat gactatcttc atcatttttg taggaagaat
     5161 gttgattaag ataaagtgta ttcatttgag catattcaaa taagtagcca ctcttatttt
     5221 tttgtaccaa aggaaattgg ttcgtaagtc gcttcttacc ctttataatt aacaatactt
     5281 tcccatattt ttctgtattt gtttcaaatt ctaaatatcc ccaagtctgt cctgctaatt
     5341 gtaatttata ctcaaacaaa tctgctgatg caaatgcagt atcaatatga ttaggtcgcg
     5401 tccatgcata accattcgac actatcattg tctctctttt ttctagacgt tcatctacat
     5461 aatctttttt ctagacgttc atctacataa tctttttgcc ctttcatcaa agtatctaca
     5521 attttttgtg cctcaagcga atcaaagaga tcctgattca acataa
//
LOCUS       DAVKDZ010000111         5556 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000111 DAVKDZ010000000
VERSION     DAVKDZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.112"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..>5556
                     /locus_tag="VWZ30_002005"
     CDS             <1..>5556
                     /locus_tag="VWZ30_002005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335664.1"
                     /translation="ITPTTASDIVKVDSDSNADGDPGTSDEEDSSKVSTRQPLELKSV
                     DELKGQEAPVEENNQVQAAAESETTGVESNSAQPETNPASQEAPQAEGEGKQDQSTHV
                     SNQTSSTSLLQPRTLKNKQSDYDSIPLGDDEDDEDDVRNGLFSSENPVTTIQPRSPRS
                     VTSRSVPPKTTGSSPYNDKYRYYFERGQDPENSQLPRYTYAFFNEKVIFGLEEATKVS
                     HIKDYLKEEIKPTTDGFSWKVTVNQARQNLNGISFLFSVPNGQSLVPNSVTVTQVDDA
                     GTTVKSSDPGDRNDDHITASLKATNANAVMKGTPTRTNASGGDGFVGSTGHYDVSSID
                     RIWNEANLPDKDSFHSRGQGNNNNARTGLQNDEERKLGDSKKMIINSSGGTVYSGKIA
                     GNTSYTITFKTRGSNDLDKLAYFSAVKGSNGGKTYLAMLLHARTQGERDFADKIRFRL
                     KGNGYYQVEQNTAYYTSTVLNSTGQEIKKDGANTAYSYSKYKDKPLDNGVKGVLTGND
                     TNDTDKAFDLDEYSYSEYVDDQGQVLNKDDLSAQLEEQQQTITWYKGGQQISKSDLTK
                     DAISSSGVHTYRYRVSYKDNSFNEGEIHFVTKPKKPEITTDIETKLGQTLDVTVRNIE
                     PNSEVTLYRKGANNQAGSLIGTVAANNQGNQVTFSGVNLQAGTYYVKQKAAGSWLDRS
                     NRRVEGVYSDPSSDKKVDGLRVGTTGKIIQYDTIDRKPINQQLVRDNPRNAALDHAFI
                     AESPDGIKTVDVQNPDNLPGFTTNEPSNASSPTRKGINVHVPAGNMQGTYRLVFEVVS
                     NSGTRKTFTTIINYPPVAPSFETTSEKLKGKAQETLKTAQPPIIINAKGVSVSNNKVQ
                     TGKKWKVFLVRGGFNNNNNIVPKAEGYEIIAEALPRDGDKRAIFTPDKFKVDKLGTQP
                     LRLITALVDEKTGKVDENVVSTLSESSIQATNPITQYQIRPEVQQDPDTLEVTAKVGQ
                     GTANKAKVTYFHQGLVPVSEIFTKVGNSWVKDSQNNNNITVSNNSDGTAIIHIPYGLN
                     TKNTPIAASQHEEGQSLWSENNSVIPKGDETAPKVSLGDAVLPTTENDATTPIYKVVQ
                     GSAFAPKLKVWDNIGSVKELDITDIPAGITKQKFGTTFQSQDNAKEANKYSGSTFSGN
                     VADTQAVGQYTAQITVKDASNNTATYYLRYEVLPKVEARQAKFPQVKSKALQDGENPE
                     TYIQFKNNTQNVSKPSVADVTWERQPSTATEGLDKTGVVKVTYHVTDENGVARDEVKT
                     VTINTPVYHATVNNGGVYTTTVGTNFSASTSATGGYVNSNFKPNVKYYWKSNRENNYP
                     EYGGDTRSQTADYLGKRQDTIRVFYPNSQDVQDYQDKRSEDHVITFISKPRIPSVDAA
                     SLAGKANQENQTVIVSNVTPGTTVKLYNGDTEIGSVNVPKGDNESYTTVKNNVTVTVN
                     GTLPLSDNIRAKTIYTPDNVDSGYSTSVSSTTQAPSKPSISQSPEDLVVKAKVGQDGA
                     TKVTLTYINANGVAKTVGFTKNHGNWDKDNANADATVSITNETDGLGEIQLQQDTAQA
                     GSEVTVKQKTDTSDYSPSATIKALSRLEDLRNVAQADGAVDIHVPEDATRMSLTYTPT
                     GQATPTTLEYTKNGRTWTAHNGINTYSDERKISIPKGVVEDGTTVSVIASNDSKTTTN
                     VKSKAKFEQPSEITNTIRQNGDVEVTLPTNAEEVTLSYKNKQNVMSTVTVSKSQGNTW
                     TVGNLPDGVSFRDGKVTFDYRKVNRDTPITISATRGTGEEKSQQRTKTISIPEHEVEV
                     TKITKKAGESTTNEELLAAIDVAKKKSAVLKKGTQYPTTVGNYDLEVMVTYADDSTEI
                     VRVPYEVTAV"
BASE COUNT         2058 a          998 c         1168 g         1332 t
ORIGIN      
        1 gattactcca actacggcaa gtgatatcgt taaggtagac tctgatagca acgcagatgg
       61 ggatccagga acctcggatg aggaagactc gagcaaggtt tcaactcgcc agccacttga
      121 actcaaatcg gtcgacgagt tgaaagggca agaagcgcca gttgaggaaa ataatcaagt
      181 acaagcagcc gctgagtctg aaacgactgg agtagaatcc aactctgctc agccagagac
      241 aaatccagct agtcaagaag ctcctcaagc agagggagaa ggaaaacaag accagtcgac
      301 tcatgtttcc aaccaaacaa gcagcaccag cctcctccag ccgcggacgc tcaaaaacaa
      361 gcagtctgac tatgactcta ttccacttgg cgatgatgag gatgatgagg atgacgtaag
      421 aaatgggctg ttttcttcgg aaaatccagt aaccactatc caaccacgga gccctcgctc
      481 tgtaaccagt cgctcagttc cgccgaaaac aactggttct agcccttata acgataagta
      541 tcgctactat ttcgaacgtg gacaggatcc agaaaacagc caacttccac gctataccta
      601 cgctttcttt aatgaaaaag tcatattcgg tcttgaagag gcgactaagg tttcccacat
      661 taaggattat ttaaaggaag aaattaaacc gactacagat ggatttagct ggaaagttac
      721 tgtaaaccaa gcgagacaaa acttaaatgg aatcagcttt ctcttttccg tgcctaatgg
      781 acaaagtctt gttccaaata gcgtcactgt gacacaggta gatgatgcag ggactacagt
      841 taaaagttcg gatcctgggg atagaaacga tgatcacatt acagcttctt tgaaagctac
      901 taatgctaat gcagtaatga aagggactcc taccagaaca aatgcaagtg gtggagacgg
      961 atttgttgga tcaactgggc attatgacgt ttcttcaatt gatagaattt ggaatgaggc
     1021 aaatcttcca gacaaagact ccttccactc tcgagggcag ggaaataata acaatgctag
     1081 gacaggttta cagaacgatg aagaacgtaa gcttggagac tctaaaaaaa tgattattaa
     1141 ttcctcagga ggaacagtct actctggaaa aattgctgga aataccagct atacaatcac
     1201 ctttaagact agagggtcaa atgacctgga caagttggct tacttttctg ctgtaaaagg
     1261 tagtaatggg ggtaagacct atctagctat gttgctgcat gctagaacgc aaggtgaacg
     1321 cgattttgct gataagattc gcttccgcct caagggcaat ggttactatc aagtggagca
     1381 aaacacagcc tattacacca gcacggtatt gaatagcact ggtcaggaaa ttaagaaaga
     1441 cggtgccaat acagcttact catactctaa atataaggat aaacctcttg ataatggagt
     1501 caaaggagta ttgaccggca atgatacaaa tgatacagat aaagcttttg acttggatga
     1561 atattcttat agtgagtacg ttgatgacca aggtcaagtt ttaaacaaag atgatttgtc
     1621 agcgcagcta gaagaacagc agcaaacgat tacctggtat aagggtggtc aacaaatcag
     1681 taaatctgat ttgacaaaag atgctatttc tagctctggg gttcatacct acaggtatcg
     1741 cgtttcctat aaggacaatt cttttaatga aggagaaatc cattttgtaa caaaacctaa
     1801 aaagcctgaa ataacaacag atatagaaac aaaattaggg caaacacttg atgttacagt
     1861 gagaaatatc gaaccaaata gtgaggtgac attatataga aaaggtgcaa ataatcaagc
     1921 tggttcactt ataggtacag ttgcagctaa caatcaagga aatcaagtta cattctcagg
     1981 agtcaatctt caagccggga cttactatgt gaaacaaaaa gcggcaggaa gctggttaga
     2041 ccgttcaaat agaagggttg aaggagttta ttctgatccc tcttctgata aaaaagtaga
     2101 tggactaaga gtgggaacta ctggtaaaat tattcaatat gatacaatcg atagaaaacc
     2161 aataaaccaa cagttagtaa gagacaaccc aagaaatgct gctttagacc acgcttttat
     2221 agcagagtct ccagatggaa ttaaaactgt agacgtgcag aatccagata acttacctgg
     2281 attcactaca aacgaaccgt ctaacgcatc tagccccact agaaaaggga taaatgtaca
     2341 cgtgccggca ggaaatatgc agggtactta tagacttgta tttgaagtag tatctaatag
     2401 tggtacaagg aaaacattta ccacgattat taactatcca ccagttgccc catcttttga
     2461 aacaacatca gaaaaattga aagggaaagc tcaagagact ctaaaaacag cccagccgcc
     2521 tatcattata aatgcaaaag gtgtgagtgt tagtaataat aaggtgcaaa ctggcaaaaa
     2581 atggaaagtc tttttggtta gaggtggatt caataataat aataatattg ttcctaaagc
     2641 agaaggttat gaaattatcg ctgaagcact accaagagat ggagataaaa gagccatatt
     2701 cacacctgat aaatttaagg tggataaatt aggaactcaa ccacttcgac ttataactgc
     2761 attggtagat gaaaagacag gaaaagtaga tgaaaacgta gtttctactt tgagtgagag
     2821 tagtatacaa gcaactaatc caataacaca ataccaaata agaccggaag tacagcaaga
     2881 tccagatact ttagaagtta ctgccaaagt tggacaaggt acggctaata aggcgaaagt
     2941 tacttatttc catcaaggat tagttcctgt tagcgaaata ttcactaaag taggaaattc
     3001 atgggtcaaa gattcacaaa acaataacaa tataactgtt tcgaataatt cagatggaac
     3061 agcgataatc catattccat acgggttgaa tacaaaaaac acgccaattg cagctagtca
     3121 acatgaagaa ggacaaagtc tatggtcaga aaataatagt gtaatcccaa aaggagatga
     3181 aacggcaccg aaggtgagtt taggagatgc tgttcttcca actactgaaa atgatgctac
     3241 aactccgata tacaaggttg tacaaggaag tgcatttgca ccgaaactta aggtttggga
     3301 taatataggt agtgtcaagg agttggatat aacagatatc cctgctggaa ttactaagca
     3361 aaaattcgga actacattcc aaagtcaaga taatgctaag gaagcgaata aatatagtgg
     3421 ttcaacattt agtggtaatg ttgcagatac acaagcagta ggacaatata cagctcaaat
     3481 tactgtaaaa gatgcatcaa ataatacagc tacttactac ttgcgttatg aggttcttcc
     3541 aaaagtagaa gctaggcaag ctaagtttcc tcaagttaaa agtaaagcat tacaagatgg
     3601 agaaaatcca gaaacttata ttcaatttaa aaataatact caaaatgtca gcaaaccatc
     3661 agtagcagat gtaacttggg aaagacaacc aagtacagca actgaaggtc ttgataaaac
     3721 aggagtagta aaagttacct accatgtgac agatgaaaac ggtgtagccc gagatgaagt
     3781 taaaactgtg acaattaata cgccagtcta tcacgcaact gtgaataatg gtggtgttta
     3841 tacaacaaca gtaggaacta acttctcagc ttctaccagt gcaactggag gatatgtaaa
     3901 ttctaacttt aaacctaatg ttaaatatta ttggaaatca aacagagaga ataattatcc
     3961 tgaatatgga ggagatacac gtagccaaac agcagattat ctaggaaaac gtcaggatac
     4021 aattcgagtt ttctatccaa attcccaaga tgttcaggat tatcaagata aacgttcaga
     4081 agaccatgtg attacgttta tttctaaacc gcgtattccg tctgtcgatg cagcgtcact
     4141 agctggaaaa gcgaatcagg aaaaccaaac agtaatagta agtaatgtta caccaggaac
     4201 aactgttaaa ttatacaatg gcgatactga gattggttca gtaaatgtac cgaaaggtga
     4261 taatgagtct tatacaactg tcaaaaataa tgttacggta acggtaaatg gtacattacc
     4321 actttctgac aatattcgtg caaaaacgat atacacacct gataatgtag actcgggata
     4381 tagcacgagt gtaagcagta cgacgcaggc accatcgaaa ccaagcatct ctcaaagtcc
     4441 agaagattta gtagtgaaag cgaaagtagg gcaagatgga gctacaaaag tgacgcttac
     4501 ctatattaat gcaaatggag ttgctaaaac agtaggattt acgaaaaacc atggaaactg
     4561 ggataaagat aatgcgaatg ctgatgctac agtatcgatt acaaatgaga ctgatggtct
     4621 aggtgaaatt caacttcaac aagatacagc acaagcaggt tctgaagtga ctgttaaaca
     4681 aaaaacggac acatcagatt acagcccttc agcaacaata aaagcgctaa gtagattaga
     4741 agatctaaga aatgttgctc aagcagatgg ggctgtggat atccatgttc cggaagatgc
     4801 gactcgtatg agtttaactt atacaccgac agggcaggct acgccgacaa cattagaata
     4861 cacgaaaaac ggtagaacat ggacagctca taacggaata aatacttatt ctgatgaacg
     4921 taaaatatcc attccaaaag gtgttgtaga ggatggtact acagtttcgg ttattgcatc
     4981 aaacgatagt aaaacaacta ctaatgtaaa aagtaaagcg aagtttgaac aacctagtga
     5041 aataactaat actatacgtc aaaacggaga tgtagaggtt acattaccaa caaacgctga
     5101 agaagttaca ttaagctata aaaataaaca aaatgtaatg tctacagtaa cagtatctaa
     5161 gagccaaggt aatacttgga cagtaggaaa cttaccagat ggagtaagct tcagggatgg
     5221 taaggttaca tttgattata gaaaagtaaa tagagacact cctattacaa tatccgctac
     5281 tagaggaaca ggagaggaaa aatcacaaca gagaacgaag acaatctcta taccagagca
     5341 tgaagttgaa gtgacgaaaa tcacgaaaaa agcgggcgag tcaacgacaa atgaagaatt
     5401 acttgctgcg attgatgtag ctaagaaaaa atcggctgta ttgaaaaaag gaacgcaata
     5461 tccaacaact gtaggaaatt atgatcttga agttatggta acttatgcag acgacagtac
     5521 cgagatagtt agggtgcctt atgaagtaac ggcagt
//
LOCUS       DAVKDZ010000112         5522 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000112 DAVKDZ010000000
VERSION     DAVKDZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.113"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..320
                     /locus_tag="VWZ30_002006"
     CDS             <1..320
                     /locus_tag="VWZ30_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU9335665.1"
                     /translation="DHPQVAKFAGLNWREKQSGNSNSQHTSLVNRGNRYLRYYLVEAA
                     NSVRRYDDEYKDFYKKKYHEVPKHQHKRAIVLTARKFVRLVVVLLRNHQLYTPPRRLM
                     EDN"
     misc_binding    complement(563..780)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(863..3559)
                     /gene="ppc"
                     /locus_tag="VWZ30_002007"
     CDS             complement(863..3559)
                     /gene="ppc"
                     /locus_tag="VWZ30_002007"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU9335666.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAETLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMNQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     gene            complement(3579..4808)
                     /locus_tag="VWZ30_002008"
     CDS             complement(3579..4808)
                     /locus_tag="VWZ30_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9335667.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            complement(4964..5190)
                     /locus_tag="VWZ30_002009"
                     /pseudo
     CDS             complement(4964..5190)
                     /locus_tag="VWZ30_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         1720 a         1163 c          961 g         1678 t
ORIGIN      
        1 aagaccatcc ccaggtcgca aagtttgctg gtctgaattg gagagaaaag caatctggta
       61 actcaaactc acaacatact tcgcttgtga ataggggcaa tcgttatctg cgctactacc
      121 tggttgaagc cgccaactcg gtaaggcgct atgatgacga atacaaggat ttctataaga
      181 agaaatacca tgaagtacct aaacaccaac acaaacgagc catcgtacta accgctagaa
      241 aatttgtgcg tctggtggtt gtgctactac gcaaccatca actctatacg ccaccaagga
      301 ggcttatgga agataactaa gttatatctg cacctctcct tgaaaatcta ctgaaaacag
      361 tgtgaaaacg tcgttttcag cagggcttct ttagtgcacc ctttttttga aatagttttc
      421 aaactttgag cggggggtcg cttgacattt taccactaga ctttgtattt cctttaacat
      481 atttcactta tctaaacaag cgacgccatc gttttctttc catactaatc tccatcaaaa
      541 aaaatcctca cacaagtttt tgtgtgagga cgtcgatgcg cggtaccacc tcaattatag
      601 ggaatttccc tatcgctcag tctcgcaata acgagatgca ctgtaaggtg tgctcaccga
      661 atttttataa tttcaaattc taaatagcat tcagcccaat tttcatcatc gccacttatc
      721 tgttttcagc taccacagac tctctaaaaa gtttgtatga ttacttttct gaatggttaa
      781 attatatcat ttttaaacta cttgtcaata gtctattaga atttttttga tattcacttt
      841 tagcattcac tcttgaaaat tatcaacctg aattacgcaa tcctgtcgca attccgttga
      901 tggtgatatg aatcaatcgt tcttgatcac tggacaattc tccacgacgt tggcgtttaa
      961 tcaactctaa ctgaatatag ttgagaatat taaagtaagg catacggtaa tccaaactag
     1021 cttttagata tggattgtca gctaagagtt cgtcatgtcc ttcaatagcc aagataacgt
     1081 tcttagtaac ttgccattca tttaaaatag tctcatagat ggccttaact tgctcgtctt
     1141 cacaaagttt agcatattca aaagcaatat tcatatttga ttttgacaaa accatatcaa
     1201 catttgaaag aagcgattgg aagaaaggcc aattttggta catatctcgt aagatagcaa
     1261 tattctctgg atttttattg ataaattcct tgaagcttga accaaccccg taccatccag
     1321 ggaacataac acgactctgt gaccatgaga atacccaagg gatggcacgc aaaccaccga
     1381 tttcagtaat agtcttacga gcggctggac gagaaccaat attaaaactt gaaatagcct
     1441 tgattggact tgactcgaag aaataatcat agaaatgctc attaccaaag accaaatcac
     1501 ggtagatatc gtaactacgg tccactactt gattcataat agcttcataa cgatttgggg
     1561 tattggtatc gctcttcttc tgagtaatca tacggttaat agctgccgat actagcattt
     1621 caaggttata gtaagcggcg tctttgttac cgtatttatt cccaattact tcaccctgct
     1681 ccgtcaagcg gatacgatcc ttgatagact tgagcggttg agatgtaatg gcttcatagg
     1741 ttggcccacc accacgaccg acagtaccac cacgaccatg gaagaaggta accttaacgc
     1801 caaattcatc tccaatagca gtcaattgtt gttgagcctt gtagagagtc caacatgaag
     1861 acaagtaacc gccatcttta ttactgtcag agtagccaag catgatttct tggtagttat
     1921 ttcgtgagtc aatccatttt ttggcaaggc taagagaaag atattttctc attgtttcct
     1981 ctgaatgatc caagtcttca attgtttcaa aaaggggaac aatctgaaca cgagcccttt
     2041 ccgtatccac cagtcctact tcttttaaca gaatagctaa ttctagcata tcagaaagac
     2101 tggttgcatg tgaaatgatg gtctgacgga tgacatcatc tcccaactta tctttcaaaa
     2161 cacgagccgt cttaaaaata gctagttctt ttgctaataa ttctgatttt tctgcgtgag
     2221 tcgcagaaag aattcgagga tcttcttcta attctttcaa gagaaggtca cacttttctt
     2281 cttcgctcaa ctcgctataa cgagaatgaa ttcctgctga tttcaagagt tctgccacac
     2341 aggcttcata gacgctagag tcttgtcgca tatcaattga tgctaggtaa aaaccaaata
     2401 tctctactgc ctgcaataat tccacaaaat cacctgaaat caaggactcg cccttatttt
     2461 ctagtagaga atctcgaatg gcaatcaaat ccttgtagaa atcattggcg gtttcataac
     2521 gagtcccaac ttcttcatcc tcaatcagat aggttttagt tgcctgaatt tttgattgaa
     2581 tatcaaacaa ggcacgacgg taaagctctt tttcgcggta aatcgagtta tccttggatt
     2641 gacgagccat ttctctgact tgcttgctga cgttgacaat gctagttgaa agagaaaatt
     2701 cacgataaag ttggtaaatc tttttatcat agtagttcat gatgacttca cattgagtga
     2761 gtgcagactg cttcaaggtc tctgctgtaa caaatggatt tccatcacgg tcaccaccta
     2821 tccacatacc catggtgatt ggtttagctt gttttaaatt cagaccatgc gcttgcgcta
     2881 agcgcttata ctccgtcgtc aaatgaggta cagctttcaa aaaggagctg ttataatatt
     2941 ccatagcatt cgtgatttcg ttagtcactt ttaatttttt ctcacgaatc atgtctgtct
     3001 gcatgataat ttcgatgtaa cgacgcaaat cattgtacca tttatcttta ttgatcaacc
     3061 ccaacttaac atcacggtat ttacgcaaaa gactatgaat atgatttgtt aaatccaaca
     3121 tacttttgcg ttgcacttgt gttggatggg ctgtcaaaac agggacaaca ttcaagtgtt
     3181 ctaggatctc aacggcattt tccttttctg ctaccaattt aatcgttgta gataatttac
     3241 ctaaatagtc ctgatcaata ttattttgat gattgatttc ataagctaaa tccacatcct
     3301 ctgaaatatt aatcaaaaga ggtaagatag agaaatagcg tgaaatatag accatttcat
     3361 catttgataa gctagtcact agacggttta gaccttgata atcttcctgc gttgataatt
     3421 ctttcaactg tattattttt tcaaaggtct ctggggcaag catattttta gtaatatctt
     3481 ccagtaattc tgttagaatc aagacttctt cttgcacaac acttttatta ctataatttt
     3541 ctaatttttg aagagacata aactatcctt tctttatcct acttcaacag aaggttcatt
     3601 ggttgatttt ccaattctcg gtacaactta gcgcgttttt cactggcatc aatatttaag
     3661 acaaaggcta ctgccactga taagactaga agactatttc caccctggga taagaagggg
     3721 aaagtcactc ctgtagatgg aatcaagccc gaaatccctc cgatattgac aaatacctga
     3781 accaacatca tccctccgac accgagcgca accatggcat taaaaggatt ctctgctcgg
     3841 atgccgacca agataatccg caaaatcatg aaaaacaaga gagctaaaat aagactggca
     3901 ccaacaaagc caaattcttc aatcacgata gaaaaaacaa agtctgtatg agcttctggc
     3961 aaataacctc gtttttcaat cgagttccca agacctagac caaaccaacc gccattgacc
     4021 atggcaaaat aagaattagc taactggtga cccgcatcag cacgatcggc aaaaggatta
     4081 aaaaaggcac taaagcgctt ggctacatag ccaaatactg gaatttttga aaaggtctca
     4141 acaccgatta ggctgatagt ggtcaagaca aagacagaag cggcagatac gagcgccaga
     4201 atggttgaaa accagcgata agcgattcca ctaactgtat acataatcaa ggaaaccaag
     4261 actaaaatag tcgcatttcc taaatcaggg aaaatgccca aacttccaat cagaactagg
     4321 agaacgaatc gccaatcatt aaaagcacgg ggaagccatt gattttgagt caaaacttga
     4381 aaatcataag tagctatttc ttcttgctgt ttggagaatc ggtgagctaa ataccaaata
     4441 ataatgattt ttaagtactc agctggctga atagttactc ctgcaaccga aatccaaccg
     4501 tatgccccgt ttacggaaat accaataaaa cgagccaaga acaataaaag catttctatt
     4561 aatataacta aaatgattag tcgctcattt ctcaaaaaat ctagtctcaa tttataaatt
     4621 aaggcaatca gtatcaaact aacaatccaa aagattcctt ggtttcgaac caactgcaag
     4681 gcgctcttgc cttcttcaat taaaatagca ctagtggtcg aatagaccac aatcaagccc
     4741 aaaatagata aaagcaagta gggaatcaag atggaataat ttaataagtg cctcttacta
     4801 atcttcatag catcaccact ctactaggat acagacaaat ctgttccaaa ttccgtttta
     4861 ttttctagtt ttgattgcta ttataccatt ttttcagaaa gaaaaaaact cttaccttac
     4921 aatccttcga attttactgt ttttacagaa ttaaggcaga atttgaaatc gtaaaaccat
     4981 tttgaacaag agttacttca acccattggc tccgactaat taagttgctt tcgtgaatgc
     5041 taaaatcagc cgcaatttgt tcataagtgc gctattctcg catgtattga atagttacca
     5101 taaggagatc gtctaggctt aatttgggtt ttcgtccacc ttttgcgcgt ttacgttgcg
     5161 ttgataagct gtttttaaca cagctaacat ctcgtgcgct gaacacctac aaggctcttg
     5221 aaacggctat cgcttaattg tttacttgct tcatcatcca tagttttatt gtattgtatt
     5281 gtatttttgt ttcgcaggaa gtctattatt cctttcgttc caaggttttt acgcttttag
     5341 caacactaca cactccacgt gatgcgtctg tggaactcaa aaggttcggg gaaccttttg
     5401 aggattaatt gcgtttctct aacaaactca cacattcaac gtggcatgac tgatgaagtc
     5461 atcataaaaa tttcacttgc tgctctttta cgttggcagc ctgaacggtc agaagtatca
     5521 tt
//
LOCUS       DAVKDZ010000113         5371 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000113 DAVKDZ010000000
VERSION     DAVKDZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.114"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..667
                     /locus_tag="VWZ30_002010"
     CDS             <1..667
                     /locus_tag="VWZ30_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEU9335668.1"
                     /translation="NYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQI
                     IKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSSN
                     LLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNECL
                     NESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKSQ
                     QLL"
     gene            complement(848..2074)
                     /locus_tag="VWZ30_002011"
     CDS             complement(848..2074)
                     /locus_tag="VWZ30_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU9335669.1"
                     /translation="MAKTRYSGVFVDTKGKYFYETELGIDRISGKRIRKKSRKDSNGK
                     PFSTAREAYLELTRVKREYHKAHTYANYNMTVKQFILDCYIPYYKSYVEESTYAVRIK
                     NLEKIAERFYNKTLRGLTIEDIQNYRIWLLSSKDENGSGYSQAYASLIFGMFRKSLDY
                     AVEMGYLETNISKKAKAIPKGKAIVPYWTKVEFERVIAQICLDDFYEHLNFVMLWTFF
                     MTGIRVNEGMALWWNDIDFDKKRMRVHHMLILKSRKNWQRNAYTKTSDGKRIISLDDD
                     TISILKRWKDRQKEIGLGNETDFIFTYDSLPMIKSTLARIIQRYSELANVKRIQGKGL
                     RHSNASYLINEFNVSVLILSKRLGHSSPEITLKHYSHLWRGADESIAEIMSGNISIHT
                     APKTKIQFNGNQNLKR"
     gene            complement(2078..2272)
                     /locus_tag="VWZ30_002012"
     CDS             complement(2078..2272)
                     /locus_tag="VWZ30_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEU9335670.1"
                     /translation="MKPLISKDDLIKLGYKKHTAISIIQAVKQDLVYNKGIPFYNNRR
                     LGLVPRERVENLIGVKLDEE"
     gene            complement(2280..3092)
                     /locus_tag="VWZ30_002013"
     CDS             complement(2280..3092)
                     /locus_tag="VWZ30_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335671.1"
                     /translation="MTRELYNTKIIQYNSNTFEVIYYRKAKFRRLGETDTTVSNTSKS
                     PSEKELYQQANRIKRRIRHYMLCNDFDLFWTLTFNDEKINSHDYLIVKKKIRTWLKAQ
                     REKYGKFHYIFIPELHKNGRLHFHGVTGGFSPKLTKARNTKTGRLINKNGKQVYNVDS
                     YQLGFSTVTKIDSSEKVANYITKYITKDLLAIPSGYKQPKYFSSRGLIKPIVSYENLE
                     RNYFEGLTPSFSVGHLDNLEYKEDIAIYNLSIDDNEEIIQNSTDTRIKIRKE"
     gene            complement(3196..4605)
                     /locus_tag="VWZ30_002014"
     CDS             complement(3196..4605)
                     /locus_tag="VWZ30_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEU9335672.1"
                     /translation="MIVSKIKRKLSKYDCREYTPTYLSVSETIESRKERQYSLIKKLL
                     KVFLISVIITFLSSHFPLLSTIYKISIVISSVLLDIFIWYIVVKFLFSTYSPIWKYRF
                     LSILRLATERLDLIQLPSNKIQIKTVEWKFYKENERIIIELNPSGTIPNIQQVKEITR
                     RLTEFLIQATNEDWNILESTVQNSYVVIKYGKSNPRYTVSELFKITDEDYYINYKPIK
                     LYDNICFDIASEANQQIIIAPSGAGKSLYLATLAGLLIKQGHSISLIDAKQTSLGATF
                     ENLGIPVARNAEEIILLLEQMVFEMEDRYKRYFAFSSVDFGTTYKHFSLPAHYLIFDE
                     VLAALECGTSAQQKEMVRLLKILALKSRASGQGLLVLASQKLLASDLPRAVTEQCQTR
                     IILGNSSSISEETFYSVMGKEKIDLLAEYRGGVGKGYILTPKTNGIKYFETPYFDLDS
                     IDFKNKIRDFQTIPRKQIE"
     gene            complement(4602..5039)
                     /locus_tag="VWZ30_002015"
     CDS             complement(4602..5039)
                     /locus_tag="VWZ30_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335673.1"
                     /translation="MENNFGKVSFNSPSLKNLLIGTSNVEHTPTSAFVIECRDVPKRN
                     EKNEVIDGTISKKVLLALQSEIVKAIQEVGGDLSSIKPFTIEIYNDESFLKKINNELI
                     IAKTIDLEGALLALKWVSDGRNGGAYREFKLIISEIKLSGAKS"
BASE COUNT         1714 a          916 c          807 g         1934 t
ORIGIN      
        1 taactatatt ctagcaaaag agaaaaatgg agttcccctt tcagatgttt ggaatatacc
       61 atttcttaat ccaaaagcta aagaaagagt aggttatcct acacaaaaac ctattctgtt
      121 attagaacaa attataaaga ttgctactga taaaaatgat atagttttag acccgttctg
      181 tggaagtgga actactttag tagcctccaa gattttgaat agaaattata tggggattga
      241 tttatctgag gaagctatca atataactca gcaacgtctg gaaaatgtta taaaaacaag
      301 ttcaaattta ttgaataaag gaatcgaagc atatagaacc aaaactgagg aagaggaaaa
      361 cattcttaaa ttattacagg caaaaattgt tcaaagaaat aaaggaattg atggtttttt
      421 acctaaacat tttcaaaaaa aaccgatacc tataaaaatt caaaaaaata atgaatgtct
      481 gaatgagagt atctctttat tacagaatgc tataaactcc aaaaaacttg attttggagt
      541 agttataaaa actcattcag ataatttatt atttgatttt gatacgattc ctgaaaatat
      601 aatagtagtt gaccattttg aacttacaat cgaaaaatgg ttaagtaaaa gtcaacagtt
      661 actatagttt gcaaattatt tacagaaaga acaaaaaatg ctctgaaaca ctgatttcaa
      721 ggcatttttt gttagtttaa aattactccc actcaattaa caatttcata ggtttagtgc
      781 cagaaaacct tgatttatta aggtttacaa tgccttttaa atatatttgg cgggatttct
      841 ggcggggtta tctttttagg ttttgattcc cgttgaattg aatttttgtt tttggagctg
      901 tatgtatgga tatattccca ctcattattt cagcaataga ttcgtcagct ccgcgccaaa
      961 ggtgtgagta atgtttcaaa gtaatttctg ggctagaatg acctaacctt ttcgacaaaa
     1021 ttaagacaga tacattaaac tcgtttatca aataagaagc gttactatga cgaagtcctt
     1081 taccctgtat tcgctttaca ttagctagtt cagaatatcg ttgaataatt ctagctagtg
     1141 tagattttat cattggaagg ctatcatatg taaatataaa atcagtttca ttgcctaaac
     1201 caatttcttt ctgtctgtct ttccaacgct ttaaaataga aatcgtatca tcatctaggg
     1261 aaataatacg tttgccatca cttgttttgg tataggcatt tctttgccag tttttccttg
     1321 attttagaat aagcatatga tgaacacgca tacgcttttt atcaaaatca atatcattcc
     1381 accaaagtgc cattccttca tttactcgaa tacctgtcat gaaaaaagtc caaagcataa
     1441 cgaaattaag atgttcataa aaatcatcaa gacagatttg agcaataaca cgctcaaatt
     1501 ctactttagt ccagtatgga acgatagcct ttcctttcgg aatagctttg gctttcttag
     1561 agatattagt ttccaaataa cccatttcta cagcataatc aagagacttt ctaaacatac
     1621 caaagattag actagcgtag gcttgagagt agccagaacc gttttcgtct ttgcttgaca
     1681 gtaaccatat ccgatagttt tgaatatctt caatagtcaa accacgtagt gtcttgttat
     1741 aaaatcgttc agcaatcttt tcaagatttt tgattctgac agcataagta ctttcttcca
     1801 catacgattt ataatatggt atataacaat ctaaaataaa ctgcttgaca gtcatattgt
     1861 aattagcgta agtatgagct ttgtgatact ctcgttttac ccgagtcaat tctaaatacg
     1921 cctctctggc agtagaaaaa ggcttaccgt tgctatcttt tctacttttc tttctaatac
     1981 gtttaccact gattctatca atgcctagct ctgtttcata gaaatatttt cctttcgtat
     2041 caacaaacac accgctataa cgtgtttttg ccatttgtta ttcctcatct agttttacac
     2101 cgataaggtt ttctaccctt tcccgtggga ctagtccaag tctacggtta ttgtagaatg
     2161 gtataccttt attatacacc aaatcctgct ttaccgcttg aataatacta attgcagtat
     2221 gtttcttata tcctagtttt atcaagtcgt ctttactaat caatggtttc atacgcttac
     2281 tattcctttc tgatttttat tcttgtatca gttgaatttt gaataatctc ctcattgtca
     2341 tctattgata gattgtaaat cgcaatatct tctttgtact caagattatc taaatgacct
     2401 actgaaaaac tgggggttaa tccctcaaaa taatttcttt ctaaattttc atagcttact
     2461 atcggtttta ttaaaccacg agatgaaaaa tattttggct gtttatatcc acttggaata
     2521 gctagtaaat cttttgtgat atattttgta atgtagttag caactttttc agaagaatcg
     2581 atttttgtaa cagtactaaa tcctaattga tatgaatcaa cattatagac ttgcttgcca
     2641 ttcttattta tcaatcgacc agttttagta tttcttgctt ttgttaattt tggactaaaa
     2701 ccgcctgtta ctccatgaaa atgaagccgt ccatttttat gtaattctgg tatgaaaatg
     2761 tagtgaaatt ttccatattt ttcacgctga gctttgagcc aagtgcgtat tttctttttt
     2821 acgattaaat aatcatggga attgattttt tcgtcgttaa aagttaatgt ccaaaataaa
     2881 tcgaaatcat tgcacagcat gtagtgacga atacgccttt ttatcctatt ggcttgctga
     2941 tagagttctt tttcacttgg acttttagaa gtattagata ctgtagtatc ggtttctcct
     3001 agtcttctaa attttgcttt tcgatagtat atgacctcga aagtatttga attatattga
     3061 attatttttg tgttatataa ttctctagtc attagtcctc cgcttatcat gcctgcatgt
     3121 caagtagcag gctaatatac acatctagcg agtcctggct tgcttatgag caagccggct
     3181 cactagatag ttagttcatt ctatctgctt tcttggaata gtttgaaagt cccttatttt
     3241 atttttgaaa tctattgaat ctaaatcaaa atacggagtc tcaaaatatt ttattccgtt
     3301 tgttttagga gttaaaatat atccttttcc aactcctcct ctgtactccg ccaaaagatc
     3361 tattttctct ttacccatga cactgtaaaa cgtttcttca gatatactgg aactattccc
     3421 taaaataatt ctagtttggc actgctctgt gactgcacgc ggtaaatcag aagcaaggag
     3481 cttttggctt gcaagaacaa gcaatccctg tccagaagcc cgtgatttta aggcaagtat
     3541 ttttagtagc ctaaccattt ctttttgttg cgctgatgtt ccacattcca aagctgccag
     3601 cacttcatca aaaattaaat aatgtgcagg taaagaaaaa tgtttgtacg tggtcccaaa
     3661 atcaactgaa gaaaatgcga aatatcgctt atatctatct tccatttcaa ataccatttg
     3721 ttctagaagt agaataatct cttccgcatt acgtgcgaca ggaataccta gattttcaaa
     3781 tgtcgcacct aaagaggttt gtttagcgtc aattaaactt atagaatgac cttgtttaat
     3841 tagtaaccca gctagtgtgg ctagataaag tgacttccca gcaccactag gagctattat
     3901 aatctgctga ttagcctcac tagctatatc aaaacaaata ttatcataaa gttttattgg
     3961 cttataattg atataataat cttcatctgt tatcttgaat agttcactaa cagtatatct
     4021 tggatttgat tttccatatt ttataacaac gtaactattt tgcacagttg attccaagat
     4081 attccagtcc tcgttagtag cttgaataag aaattcggtc aaccgccttg ttatttcttt
     4141 tacttgctga atattaggaa ttgtccctga aggattgagt tcaattataa tcctttcatt
     4201 ttctttataa aatttccatt caactgtttt tatctgtatt ttattagagg gaagttgaat
     4261 taggtctaaa cgttctgttg caagtctaag aatgcttaaa aatctgtact tccatatagg
     4321 agagtaagta gaaaataaga attttactac aatataccat ataaaaatat ctaacagaac
     4381 agaagatata acaatactta tcttgtagat agtgcttaat aaaggaaaat gtgatgaaag
     4441 aaaggtaatg ataacagata ttaggaacac ctttaacagt ttcttaataa gagagtactg
     4501 cctttcttta cggctttcta tagtttctga aacagataag taagtagggg tatattcacg
     4561 acaatcatat ttactaagtt tcctttttat tttagatact atcatgattt agcacccgat
     4621 aactttattt ctgaaataat gagcttgaat tctcggtacg caccaccatt tcgaccatca
     4681 gatacccatt ttaacgctaa taatgctcct tctaaatcta ttgttttagc aatgattaac
     4741 tcattattaa ttttttttaa aaaagattca tcgttgtata tctcaatagt gaaaggtttt
     4801 atgctactta aatctccacc aacctcttga atagccttaa caatttctga ctgaagagcc
     4861 aataaaactt ttttacttat tgttccatca atgacttcat ttttttcatt acgtttagga
     4921 acatctctac attcaataac aaaagcacta gtaggagtat gttctacatt ggacgtacca
     4981 attagtagat ttttcaagct tggtgagtta aaactcactt ttccaaaatt attttccatt
     5041 tgttacctct ttatctttgt gtgcacacta gattaatttc atcaagcagt tcatgacctc
     5101 cttgacagtc atattgtctc atttttaatt agttgaaatg tggggataaa aattttttga
     5161 caacaaaaaa atccccacgc gaagtgggga aaatatgcta tacagtacta aataaaaatt
     5221 ttattattta cgataaatta aactttcgag tgttagttta tctatcattg gtaataaaat
     5281 atactttgct aataagtcta acttttctgg aagtttttga tagctaagat aagattcctt
     5341 atttttaggt gatagtgtga cagagtcctt t
//
LOCUS       DAVKDZ010000114         5260 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000114 DAVKDZ010000000
VERSION     DAVKDZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.116"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            519..698
                     /locus_tag="VWZ30_002016"
     CDS             519..698
                     /locus_tag="VWZ30_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335674.1"
                     /translation="MMMNTSNDLFIKAIELLPFYSLPVFVASNTMSISQYLVVITTII
                     LLCFFTLKKSKKYSF"
     gene            1084..1293
                     /locus_tag="VWZ30_002017"
     CDS             1084..1293
                     /locus_tag="VWZ30_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9335675.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            1283..1771
                     /locus_tag="VWZ30_002018"
     CDS             1283..1771
                     /locus_tag="VWZ30_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335676.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            1887..3347
                     /locus_tag="VWZ30_002019"
     CDS             1887..3347
                     /locus_tag="VWZ30_002019"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU9335677.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            3344..4168
                     /gene="nadE"
                     /locus_tag="VWZ30_002020"
     CDS             3344..4168
                     /gene="nadE"
                     /locus_tag="VWZ30_002020"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU9335678.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            4417..4968
                     /locus_tag="VWZ30_002021"
     CDS             4417..4968
                     /locus_tag="VWZ30_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9335679.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSICNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
BASE COUNT         1568 a          939 c         1142 g         1611 t
ORIGIN      
        1 cttaaggaaa atataagaaa tttatattca tcaggcgtaa tatatctcgg tttgcttatc
       61 tcgtttatac cgccgatatt ggttacattc tttattctaa agactcaagg gacatcgctt
      121 ggtattaagc atatttcaaa cttttatgct atgctcggta tgttaatggc tgttatacat
      181 gctaatcgag tcattagtag agatttttcc cacaatacgg taagtttgtt ttataatcaa
      241 cagaagaatc ggatgattta cgtcttgtct aattttctat atgccatctc agtttccatt
      301 atttatgctt tgaatggcat tgtgttacta gtcatagtaa gtaaattggg tattccaggt
      361 gatttaggat tagattttat agtagctatt gtagtcaata caattttgtt agtcctgttt
      421 tattttctat tatcttacat tttctattta tacaaattga aaagtggctt ggtattttag
      481 tagctttact actctttatc cctaatatat taaatacgat gatgatgaat actagtaatg
      541 atttgtttat caaagcaatt gaacttcttc ctttttattc tttacctgta tttgtggctt
      601 caaatacgat gtctattagt cagtatcttg tggtaatcac tacaatcatt ttattgtgct
      661 ttttcactct caagaaaagc aagaagtatt cattttagtt tcatttagta ttattttgca
      721 tacctaaaat acaataaaaa aatcagtcat cttggtatgc tcctgctttc actattcaac
      781 acgtttttga cttatactag gctcatttcc aaaagcatta tataatagtg atatgaaacc
      841 aactaaacta aacaagaaat ataagcaata aaaactcgtt taaaagatcc tactaaagct
      901 aatactaaat aaaaataaaa gagtaaacta ggaagtttat ttcaaacaac ctaaaatact
      961 gattttcggc tgaagataat actggagtgc aaattaatgg ggttataata aatagctgat
     1021 agcttgtgtt ggttttggat tttttaagag tagatgagta ttaaaactat aaggaggacg
     1081 aaggtggcta aaaatttaaa attaaaatta gctcgggtag agcgtgattt aacacaaggt
     1141 caactggcag aggctgtcgg ggtgacacgc cagactattg gtttgataga ggcgggaaaa
     1201 tacaatccca gtctctcgct ctgccagtct atttgcagat gtttagggaa aaccctagac
     1261 caactatttt gggaggaaga agatgaaaaa tagattttat tattatcaat tactagacga
     1321 aagagaagaa caactgttca ataaagcggg ctctgaaagt ttctatatct gcattgcttt
     1381 gtcgctccta tcttatatca tttcagtatt agcaccaagc ctttttaatt ctaatatgct
     1441 gctaatcgtt atcatcatag ggacatttta ctttttcaat cgtgcccgtt atctgggagt
     1501 gacctactat agtcgttttc attttacgat tttgggttgt tttttcctaa ccttggctat
     1561 tacggctctt ttgatgttgc agaattatca attcaacata gaaatttatc agcacaatcc
     1621 tttgaatttt aaatacctgt ctgcttgggt cattacttat atcatttacc ttccatgggt
     1681 ctttattggc aatcttggtc ttaagagcta tggcgaatgg gctcagaaaa aatttgaaca
     1741 agatatggat gaattggaga gtggagaata gcttgttact cttttctcaa tccagctaaa
     1801 atgtgatata atagtactaa tttattggaa tacatgaaag ttcttgaaaa ttttcatggg
     1861 tttctagcta aggaagtagg aaaagtatgt atccagatga tagtttgaca ttgcacacgg
     1921 acttgtacca gattaacatg atgcaggttt actttgacca agggattcac aataagaagg
     1981 cggtctttga ggtatatttc cgccaacagc cttttaagaa cggctatgcg gtttttgcag
     2041 gtttggaaag aattgtgaac tatcttgaag acttgcgttt ttcagatagt gatatagcct
     2101 atttggagtc gcttggttat catggggcgt tcttggatta ccttcgcaat ttcaagttgg
     2161 agttgaccgt tcgttctgcc caagaagggg atttggtttt tgctaatgaa ccgattgtgc
     2221 aggtggaagg acctctagcc caatgtcagt tggtcgaaac ggctcttttg aacatcgtca
     2281 actaccagac cttggtggcg acgaaggcag ctcgtattcg ttcggttatc gaagatgaac
     2341 ccttgatgga gtttgggaca cgtcgggctc aagaaatgga tgcggccatc tggggaacac
     2401 gcgcagcggt gattggtggt gccaatggaa ccagcaacgt gcgtgcgggt aagctctttg
     2461 acattcctgt tttgggaacc catgcccatg ccttggtaca ggtttatggc aatgactatg
     2521 aagctttcaa ggcttacgct gcgacccaca aaaattgtgt ctttcttgtg gatacctatg
     2581 acacccttcg catcggtgta ccagctgcca ttcaggtggc gcgtgagctg ggtgatcaga
     2641 ttaactttat gggtgtgcgg attgactctg gggatattgc ctacatttct aagaaagtcc
     2701 gtcagcaact ggacgaggct ggatttacag aggctaagat ttatgcttct aatgatttgg
     2761 acgaaaatac tatcctcaac ctcaagatgc aaaaggccaa gattgatgtc tggggcgtgg
     2821 gtaccaagct gattacagct tatgaccagc cagctcttgg tgcagtttac aagattgttg
     2881 caatcgaaga tgaaactggt cagatgcgca atacgattaa gctgtctaat aatgcggaaa
     2941 aagtctctac gccaggtaag aagcaggtgt ggcgcattac cagtcgtgaa aaaggcaagt
     3001 cagaaggcga ctatatcact tatgatggtg tggatattag cgacatgaca gaaatcaaga
     3061 tgttccatcc gacctataca tacatcaaga agacggttcg taattttgat gccgttcctc
     3121 tcttggtgga tatcttcaaa gaaggaatat tagtttacaa cttgcctagt ttgactgaca
     3181 ttcaggatta tgcccgtaaa gaatttgaca agttgtggga tgagtataag cgtgtgctca
     3241 atccgcagca ctatccagtg gatttggcgc gtgatgtatg gcaagataag atggacttga
     3301 ttgataagat gcgcaaggaa gcccttggtg aaggagaaga agaatgagtt tgcaagaaac
     3361 gattatccaa gagctgggtg tcaaaccagt gattgatgcc caggaagaaa tccgtcgttc
     3421 tattgatttc ttaaaaagat atctgaaaaa acatcccttc ctaaaaacct ttgtactagg
     3481 gatttctggg ggacaagact caaccttggc agggcgtttg gcgcaattag ctatggaaga
     3541 actgcgagct gaaacgggag acgacagcta caaatttatc gctgtccgtc tgccatacgg
     3601 agtgcaagct gatgaagcag atgctcaaaa agccctagcc ttcatccagc cagatgtcag
     3661 cttggttgtg aatatcaagg agtcagctga tgccatgaca gctgcagttg aagcgacagg
     3721 tagtcctgtt tcagacttca acaaggggaa tatcaaggca cgttgccgta tgattgctca
     3781 gtatgccctt gctggttccc atagcggagc ggtcattgga acagaccacg ccgcggaaaa
     3841 tatcacaggt ttctttacca agtttggtga cggcggtgcg gatattctcc ctctttaccg
     3901 cctcaataaa cgccaaggaa aacagctctt gcagaaactt ggcgcagagc cagcccttta
     3961 tgaaaaaatc ccaacggcag acctagaaga agacaaacca ggcctagctg acgaagtcgc
     4021 acttggagtc acctatgcag agattgacga ttacctagaa ggcaaaacaa tcagcccaga
     4081 agctcaagcg accattgaaa actggtggca caaaggccaa cacaaacgcc acttacccat
     4141 cactgtattt gatgactttt gggagtaaaa acctccattg gaggttttca gcctcttatc
     4201 ttgaaataag aaaatgaggc agattcggta actcgaagag ttcgatttca tcgtcttacc
     4261 cctgcaacga tgactaggtt tgaaaaagat tgctagagcg catttcaaac caggcagcga
     4321 ctgcgtcaag aaattagaag acaaactcgt tttctagctg ttactgagtt gagccttttt
     4381 actacgagca tagaaataag gaagtgaggt agcatcatga aatctatcgg tacgcaaata
     4441 ttacagacag aacgtttgat tttaagaaga tttgtggaga gtgatgcaga agccatgttt
     4501 caaaattggg cttcatccgc tgagaatctg acctatgtta cctgggatcc ccatcctgat
     4561 gtcgaaatca ctcgaaactc gatttgcaat tgggttgctt cctatactaa tctcaactat
     4621 tataaatggg ccatttgtct aaaagaaaac ccagagcaag tgataggaga tatcagcatt
     4681 gttaagatag acgaggctga tttaagctgt gaaattggct atgtgttagg caaggcttac
     4741 tggggaaatg gtatgatgac agagactttg aaagctatct tggacttttg ttttactcaa
     4801 gcaggttttc aaaaggtcag agcacgttat gccagtctca acccagcttc aggtcgtgtc
     4861 atggaaaagg ctggaatgtc ctatctacaa accattgtta atggtgtaga gagaaaaggc
     4921 tatcttgcgg atcttattta ttatggtata agtagggaag aatgttgaat tctattttct
     4981 gtttctatcg aagtcaacta tttattgtaa atataataat tagcattcca agtttatttg
     5041 aaactttaaa atagcatatt gattagtaca agacagatgt tctagttcct tctttaatct
     5101 ggtttagtat tagttaaaaa atcgctttaa gcttgtaact aagagggagc taatcgacta
     5161 gattctccag ccgaacaggt ggtaatgtac tttttatagt gtaatcctag ctgttgttaa
     5221 atttaaaata gaatcctcta tcgagttagg gaattaaatt
//
LOCUS       DAVKDZ010000115         5070 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000115 DAVKDZ010000000
VERSION     DAVKDZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.117"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            85..374
                     /locus_tag="VWZ30_002022"
                     /pseudo
     CDS             85..374
                     /locus_tag="VWZ30_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            397..930
                     /locus_tag="VWZ30_002023"
     CDS             397..930
                     /locus_tag="VWZ30_002023"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU9335680.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPVLYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTELLPAL
                     KTPHVIVMGNTSFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLECFFKTR"
     gene            1270..1524
                     /locus_tag="VWZ30_002024"
     CDS             1270..1524
                     /locus_tag="VWZ30_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335681.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            1635..2144
                     /locus_tag="VWZ30_002025"
     CDS             1635..2144
                     /locus_tag="VWZ30_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335682.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKPQNWQN"
     gene            2132..2741
                     /locus_tag="VWZ30_002026"
                     /pseudo
     CDS             2132..2741
                     /locus_tag="VWZ30_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
     gene            2699..3262
                     /locus_tag="VWZ30_002027"
     CDS             2699..3262
                     /locus_tag="VWZ30_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9335683.1"
                     /translation="MVPKTATSTETKIITRTIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVVSGKIDKYKDPDIPTVKSQEVTSDSSDKEITVRYDRL
                     STLEKPNPELPNQETPIPEPTPEPGTPKTETPVNPDPEVPTYETGKREELLNTGTEAN
                     ATLASAGIMTLLAGLGLGFFKKKEDEK"
     gene            3486..4355
                     /locus_tag="VWZ30_002028"
     CDS             3486..4355
                     /locus_tag="VWZ30_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9335684.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQYMKQTAIQAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVVPIAA
                     AIAAVAVFKFLYL"
BASE COUNT         1744 a          889 c         1040 g         1397 t
ORIGIN      
        1 atttcagtaa aaaaaagtaa gtcttctaat aacaaaacat tgatatagtt gcttagtttt
       61 aaacaagcat attataataa agctatggca tatagtactg attttaaaca gcgagcatta
      121 gattacatca aagaggggca cagccatgtc gaggcagcca agttttttgg tgttggcgtc
      181 agaactctct tcacgtggga aaagaaagac gtaaacaagg acacatagag aggaaaaagc
      241 gagtcgtcaa aaaccgaaag attcctttag aggaattgaa agcctttgta gaggctcatc
      301 cagatgcttt tttacgggaa attgcggcac attttgattg tgctctgccc tccgtatggg
      361 cagctttaaa gtagattaag gtcactttaa aaaaagatga cgagctttaa ggaacaagag
      421 ccagagaaag tcgctgagtt tcttgatatt ttggataacc taaaagattt accagtccta
      481 tatattgatg aaacaggaat cgaccgctac ctctatcgtc cttatgcagg ggctcctaga
      541 ggggagaaag tctatgaaaa gattagcgga cgtcgttttg agcgaacttc aattgttgca
      601 ggacaagtag acggagagtt tatagctccc atgatttaca agaaaagcat gacaagcgat
      661 ttctttgtgg agtggttcaa aacagaactc ctgcctgctt tgaagacacc tcatgttatt
      721 gtcatgggca atactagttt tcatcccaag aacattttgg atgaactctg catccaagat
      781 aaacactttt tcttacctct accaccttat tcaccggatt tgaatcctat tgagcaagct
      841 tgggctatct tgaaaaagaa agtgacggat ttattaaggg aagttccaac tatttttgaa
      901 tgtttggaat gcttttttaa aactagatga ctataacggt tctaaaggaa cctatcgagt
      961 agtcattaaa actaaggata ctgctggtta agagaagacg gtatacgatc aaaccattca
     1021 ccgtgtagcc gaaatcgttc agaatgaaga cttgtataag gaaggtttga atgttgaact
     1081 tgcgcaccaa caaattaagg gattttttga agcagagttt aaaaatcgta ttaatggagt
     1141 tcttaatact aaaataaaaa atagtacatt aaatcgtgta aataaaaaaa ctatacacca
     1201 gagcaacaaa aactccatga tcaatttgaa gcagaagcaa cggaagatgc taaaaaacaa
     1261 ggcgatattg tgttgaatgt tgaccaggat ttcatgagca tatctaagtc taataaaagt
     1321 ggttcagact ggaagaaaac tttcacagtg aggacaacca ataggctagc aaatgacttg
     1381 aataatgtct tgaaacaggc tgataaagat actcctaata ccccaacttg gctaaactca
     1441 gctgcttcta aagctaaaga tgatgacaga gcatataaac tactgaagac tcttatacca
     1501 ggagaaaatt acctatcatg ttaaggataa tcagctagaa gtagaaacag ataaatacac
     1561 atatactgcc gctagaaatg gtagtaagga agttggtatt caagagtcag atatagcagc
     1621 aactctaagt gccgatgaat ataattctaa tcgccaaact tttgagagag aatacaaata
     1681 caaaagcaaa tgcccttaat aatggttggg ctagatctgg ttctgaagag ttcaaaaagt
     1741 tctcccactt tgtaggggta gacaaaggga ttgtgcgaac gaatgtactg actggtaaaa
     1801 aactatctga taagattagg aaagaagtgg gctctggaga tagcaaacta ggaaaaggcg
     1861 gctatttctc tactggggat gttctattag gaaaagatgt tgtttcttat accgtacaag
     1921 tattttcaga gaataatgaa agagtaggag taaacactca aagtcaccgt gttcagtata
     1981 atctcccaat tctagctgac ttttcagtca tccaagatac tgtggaacca tcacgaaccg
     2041 ttgttgaaaa aatcattcca aaactaaata ttcccgaaga agagaaaggg aaaataaccg
     2101 aagaaatcaa gaaaaagaaa aaacctcaga attggcagaa ctaatctcag aaaatgtgaa
     2161 agttcgctat gttgatgaac aagggcgttt gctatcattg aaaaatgata ctggaattgg
     2221 agaaaaagaa agtgacggaa cctacattac caataaaaaa acaactgatt ggtaccagct
     2281 ataatgtcac agataaaaaa ctcagtagca tgactactac tgacggaaaa tattatactt
     2341 ttaaagaagt agatacaaat tctgcaagtt taactgggaa tattgtaagc gaaggtagaa
     2401 cagtgacctt agtttataga gaaagcgaag cgccaaccac tgttacagta acagccaatt
     2461 actataaaga aggtagccaa gagaagttgg cagagtctgt tataaaagct gatttagcga
     2521 taggttctga gtataccaca gaatcaaaaa ctattgaagg gaaaacaaca actgaggata
     2581 aagaagaccg agttatcaca aggaaaacaa catacacctt ggtagcaact cctgcaaatg
     2641 cataccagaa gacggtgcaa cagttgacta ttactaccgt gagaatgttg aggaaacagt
     2701 ggttcccaaa acagcaacct ctactgagac gaagattata acgcgtacca ttcattacgt
     2761 tgataaagtt acgaaccaaa atgtaaaaga agatgttgtt caacctgtaa ccttaagccg
     2821 tacaaaaact gagaacaagg tcacaggagt tgtaacctac ggtgaatgga caacaggaaa
     2881 ctgggacgag gttgtatctg gtaagattga caagtacaaa gatccagata ttccaacagt
     2941 taaatcacaa gaagttacgt cagactctag tgataaagaa ataacggtaa ggtatgaccg
     3001 tttatcaaca ctagaaaaac caaacccaga actaccaaat caagagactc caataccaga
     3061 gccaactcca gaaccaggta ctccaaaaac tgaaactcca gtgaatccag acccagaagt
     3121 tccgacttat gagacaggta agagagagga attgctaaac acaggtacag aagctaatgc
     3181 taccttggct agtgctggta tcatgacctt gttagctggt ctaggattag gattcttcaa
     3241 gaaaaaagaa gatgaaaaat aatagatttt agaatctagg aacaaggaaa agctcacaga
     3301 tgtgggcttt tttcctggtt ttgagaacga ggtctttcgt gaagaataaa aacgcttata
     3361 aatctgttga actgggaaac tatgaatcct atttttttaa aaatatttcc agaaatcagt
     3421 tgctaaatag ggtaaaaatt gataaaatag ggaatatcta aaaaatttta aggagaatct
     3481 agcaaatgga tttcacatgg gcacttaagt atgccactga atttttggga actgccattt
     3541 tgatcattct tgggaatggt gcagttgcca acgttgaact taaaggtacg aaaggtcacc
     3601 aaagtggctg gatcgtcatc gctgttggtt atggtatggg ggttatgatc ccagccttga
     3661 tgtttggtaa cgtatctggg aaccacatca accctgcttt tactctaggg cttgcagtta
     3721 gcggtctttt cccttgggca caagtggtac cttacattat cgcgcaagtc ttgggggcta
     3781 tctttggtca agccttagtt gtggcaacat accgtccatt ctatttgaaa actgaaaacc
     3841 caaataacat cttgggaact ttctcaacta tttcaagtat tgaccatggt acaaaagaaa
     3901 gtcgctatgc agcaactgtc aatggtttga ttaatgagtt tgttggttca tttgttttgt
     3961 tctttgcagc tcttggtttg actaaaaact tctttggtgc tgaagtgctt caatacatga
     4021 aacaaacggc aatacaagca ggacaaacag ttgatttttc tgacttggct attaaagcac
     4081 aggtggctcc acacactgct tcaggacttt ctgtggctca cttggcactt ggattcctcg
     4141 ttatggcttt ggtaacatca cttggaggac ctacaggacc tgccttgaac ccagcccgtg
     4201 acttgggacc acgtctcctt catgctttcc ttccaaaatc agttcttggt gagcataaag
     4261 gcgattcaaa atggtggtat tcttgggtac cagtagtagt acctatcgca gcagcaattg
     4321 cggcagtagc tgtattcaaa ttcctttatc tctaagaaat agctccttta acatttgagt
     4381 gagcaccatc tataagtaag agaggatcag actggttctc tctttttgat tttttaggga
     4441 aatgaaagaa actctaaaca aactcctctc cagcagtggt ttagaagtct cagtgggcta
     4501 ttccagcttc aatgggctac tatagtgggt tgaagttgga atagtccccc cttctttctc
     4561 aaacattgtg aggaattgat ttaccttcct caacaaaatg ttcagtttct atttcatttt
     4621 gctataaaat aagcgattag gggggctatt cttcgaccta cattgactct gctgagtcct
     4681 atgattgtta tcgttttatc tgcaatttta tactcaatga aaatcaaagg gcaaactaag
     4741 aagctagccg caggttgttc aaaacacagt tttgaggttg tatagtagat tgaaactaga
     4801 ataatacaca tctacttcta aaacattgtt agaaatcgat ttgactgtcc tgatcgattt
     4861 gccctattct tgtttcattt tactatataa accagagact gtttacattt tcagcaagtg
     4921 agtggatgga taatgctgaa aactccttga aggataagtc tatttagtac tttctattaa
     4981 ttagttaaat ttttaccaag aataattcac aaaaacgttg taaaacactt gcaatttagc
     5041 tgaaatttga taaaatagta aggaaagtta
//
LOCUS       DAVKDZ010000116         5064 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000116 DAVKDZ010000000
VERSION     DAVKDZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.118"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(<1..391)
                     /locus_tag="VWZ30_002029"
     CDS             complement(<1..391)
                     /locus_tag="VWZ30_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335685.1"
                     /translation="MKSTIKNYYEMWTKRHERRYREALGYYNQQSKEALLAELTELEI
                     KKERSSRSWKLLVGLVLTIIFSDDLKRLLAILFSPLTGVPTNKLHESVLPSISIALFI
                     VLLFLTILIYNFSYSLLLRRYLILKRYI"
     gene            complement(732..2567)
                     /locus_tag="VWZ30_002030"
     CDS             complement(732..2567)
                     /locus_tag="VWZ30_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEU9335686.1"
                     /translation="MITEKKKRRLRPYLLLGIGLFYFFHWLFKLWLLAPDTDSVTDVF
                     GLGKLNWMSDHLNDKSWFDFQFTPTSLLIGMSGFLIAFLLYLRVTDTGTYRYGEEHGS
                     ARFATREELMRFRDEEPEKNMIFTQNSQMGLFNNRLSFENQINKNILVYGGTGDSKTR
                     SAVKPNILQGNSSFVTTDTKGILIHETGKSLVEKGYKMKIFDLITFLNSDGFNVFRYI
                     HNEMDIDRVAEAITESLNRNGHESDPFWPAANKLLMRSLIGYLYFDGQIDHYLPNLGQ
                     VTDMIRELRRNHPEAESPVELMFEDLERRSPGNYACRQWKLFNKNFDGQTRTSVYAIF
                     ATTFSVFDHEQLRKIIEKDTLEIEKWNIEKTAVFIHIPEVDPAYQFLSALLFSTVFDV
                     LIKTADAVILGEHPTKIKEDLLHLQVWADEFGQIGKIPNLPPIISVIRSREISIKMMV
                     QSQSQIEVLYGKENTKTIINNCGAILYLGSNDLDTLKYLSERSGKQTLNDQNYSESRG
                     RNASSSKQNSKIGRELLTPHEVATIGTTEALLFLSKQNVFRDQKFNLDTHPRAYLLSN
                     GSNDDNWYRYKRYLSDIDEWKAQVGEENVIYIGIKEVEEVPLKVS"
     gene            complement(2564..3112)
                     /locus_tag="VWZ30_002031"
     CDS             complement(2564..3112)
                     /locus_tag="VWZ30_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335687.1"
                     /translation="MEQEQVVAQLGRATLVTGEIILKGLFLVSSKAVEMYQARGENVL
                     FTGETDWNKFMATADTKEVQQLLNNEVNLEAVRKELGQYGIGFSFYTHPDGKTTLAFN
                     AKDKGVVEQAFKDVLEGITRDPRGFNERNLKNGKTTSFEEKLQHFKAVEQEKISSLQV
                     NIHTKEFVLSENIEPQIGGKMK"
     gene            complement(3114..4013)
                     /locus_tag="VWZ30_002032"
     CDS             complement(3114..4013)
                     /locus_tag="VWZ30_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335688.1"
                     /translation="MYKIINQLTNQEETFEYRDDLLSRLEIINERMKSNKINGIYRLY
                     SLNSEGEILQEESLEIPFVGIIDQLLENFGMSAGKKKKGLLHFLEKHKEKNSSLGTPE
                     IQLQPEFQPEPDPELEDLTVTKEANMARQIIAELVSQKPRDEVREEVPLEDKMTYSSS
                     EWTTDHFSRLDENEEERVEPSQDMPTQEVVSIVEKSTQEEKIPLSVKEDKGNVETSFS
                     LSHVTSVNLPSEVIDSFEAQTVTYKQSIKHKIQSNEEFIKEANEEINECQNKINELRQ
                     QILAKEKQASQLKDLYFKIEEVS"
     gene            complement(4006..4245)
                     /locus_tag="VWZ30_002033"
     CDS             complement(4006..4245)
                     /locus_tag="VWZ30_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335689.1"
                     /translation="MNKKIEQLEGKLEKVRKDKEKAQRQKEEADKKIKNYINQEKDLE
                     AQLFVALSEESGLSYQEMKELILPAQSSNQGVNHV"
     gene            complement(4256..4936)
                     /locus_tag="VWZ30_002034"
     CDS             complement(4256..4936)
                     /locus_tag="VWZ30_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335690.1"
                     /translation="MYNQKLIKMVKGLFVGFIAVLCIILGYSIAQVQQNLRQNSQVQE
                     EAKQKPEEKQQEQLSLTEQQVEEFLIAYYTKKDLEENRPRYKPFMTDSMYQETVQEEQ
                     KPVNQTYKGYVVDQAFESGSIYIDKQHKIALAQVQYTSTTLTEKDNRASKGLTGSTKA
                     TIRLTYVEKAGKLLINKIEPIVLTEARGQSVGTYADTSSSSSTPVSSTSTATPTSSEP
                     TTATSSNQ"
BASE COUNT         1419 a         1071 c          796 g         1778 t
ORIGIN      
        1 ctatatagcg ttttaaaatg agatagcgtc tcagtagcaa cgaatagcta aagttataga
       61 ttaaaatagt caaaaataac aaaactataa atagtgcaat actgatgctc ggtaacacac
      121 tttcatgcaa tttattggta ggtacacctg ttaaaggtga aaataaaatt gctaaaagcc
      181 gttttaaatc atctgaaaaa ataatggtta atacaagacc taccaataat ttccagctgc
      241 gagaactcct ttcctttttt atctctagct ctgtcaattc tgctaacaat gcctctttag
      301 actgctggtt atagtaaccc aaggcttctc tgtatcgacg ttcatgccgt ttcgtccaca
      361 tttcataata attttttatg gtagatttca taggattatt acactcctct caatcatcgc
      421 ttattctcca ttcattatat cacaaaatac taaaacacat tgtgctctct agttcttggt
      481 ttctatatac ccaagttcat ctgtagaaaa accaacaatc ttatagaatc ctatggctcc
      541 aaaagtttct aataaatgag tttccgctac ccttttagct gtattaaaat cacgaaaaaa
      601 acctgcatga gctaaatttt catgccaggt tattttttcg ccttcctaat gcaaccacac
      661 atactgatcc caatttatag caattaaaac gcaataagag tcataaaatt ccattagatt
      721 ccctttcttt cctaacttac tttaagagga acttcctcaa cttccttaat cccaatataa
      781 ataacatttt cttccccaac ctgggctttc cactcatcaa tatcgcttag atagcgcttg
      841 tatcgatacc agttgtcatc attcgagcca ttacttaaga ggtaagctct tggatgcgta
      901 tccaggttaa atttttgatc tcgaaagaca ttttgtttgg atagaaagag tagggcttct
      961 gttgttccaa tagtggcaac ctcatgcggt gtcaacagtt ctcgaccaat ttttgagttc
     1021 tgcttagaac tgctggcatt tcttcctctg ctctcactat aattctgatc attcaaagtt
     1081 tgcttccctg atcgctcaga tagatactta agcgtatcta agtcattgga acctaaataa
     1141 agaatagcac cacagttatt gataatcgtc ttcgtatttt ctttaccgta caatacttca
     1201 atctgacttt gagattgcac catcatcttg atagaaatct ctcgagaacg aataacagag
     1261 ataattggcg gtaaattagg aatcttccca atctgaccaa actcatctgc ccacacttgt
     1321 agatgtaata aatcttcctt gatctttgtt ggatgctcac ctaatataac tgcatctgcc
     1381 gtttttatta gcacatcaaa aacggtacta aaaagaaggg cagataagaa ctgataagct
     1441 ggatccactt ctggaatatg aataaaaact gctgttttct caatattcca tttttcaatc
     1501 tccagtgtat ccttttcaat aatctttctt agttgctcat ggtcaaacac tgagaaggtt
     1561 gtcgcaaaga tggcataaac agaagtccgt gtttggccat caaagttttt attgaatagt
     1621 ttccactgcc tgcaggcata attccctggg ctccttctct ccaggtcttc aaacatcaat
     1681 tcaacaggac tttccgcttc tggatgattc cgtctcaatt ctcgaatcat atctgtaacc
     1741 tgccccaaat taggcagata gtgatcaatc tgcccatcaa aataaagata gccaattaga
     1801 gagcgcatca ggagtttatt ggctgctggc caaaaaggat cactttcatg accatttcta
     1861 ttgagagatt ctgtaattgc ctctgccact cggtctatat ccatttcatt atgaatatat
     1921 ctaaaaacgt tgaaaccatc tgaattcagg aaagtaatca aatcaaaaat tttcatctta
     1981 taaccttttt ctacaagtga tttccctgtt tcatgaatca aaatcccttt ggtatctgtg
     2041 gttacaaaac tagagtttcc ttgtaaaata ttcggcttta cagcactacg tgtctttgaa
     2101 tcccctgtac ctccatatac cagaatattt ttattaatct gattttcaaa gctcaatcgg
     2161 ttattaaata atcccatctg gctattttgg gtgaaaatca tattcttttc aggttcttcg
     2221 tctcggaatc tcatcaactc ttctcttgtc gcaaaacgag ccgatccatg ttcctcccca
     2281 tacctgtacg tacctgtatc cgttactctc aaatacagca gaaatgcaat cagaaaccca
     2341 ctcataccaa tcaataaaga agttggtgtg aattgaaaat caaaccaaga tttatcgttt
     2401 agatggtcgc tcatccaatt aagctttcca agtccaaata catctgttac tgaatcggta
     2461 tctggtgcta acagccatag tttaaagagc caatgaaaga aatagaatag accaatgcct
     2521 aacaacaaat acggtctaag tcttcgtttc ttcttttctg taatcacttc atttttcctc
     2581 caatctgtgg ttcgatattt tctgaaagaa caaattcctt agtatgaatg tttacctgga
     2641 gggaagaaat tttctcttgt tccactgctt tgaaatgttg caatttctcc tcaaaagaag
     2701 tcgttttccc atttttaaga tttcgctcat tgaatcctct cggatctctt gtgatgcctt
     2761 ctaggacatc cttaaaggcc tgttcgacaa ctcctttatc ttttgcattg aaagcaagag
     2821 ttgtcttacc atcagggtgt gtataaaaag aaaagccaat cccgtattgg ccaagttctt
     2881 ttcgaactgc ctcgagattg acttcattat ttagtaattg ctggacttct ttcgtatctg
     2941 ccgttgccat gaatttattc cagtctgtct caccagtaaa gaggacattt tcccctctgg
     3001 cctgatacat ttcaactgcc ttagagctca ccagaaagag tccttttaag atgatttctc
     3061 ctgtcacaag agttgcccgt cctaattggg ctacaacttg ttcttgttcc attttagctc
     3121 acctcttcaa ttttaaaata aagatccttc agttgactag cttgtttctc tttggcaaga
     3181 atctgttgtc gcaactcatt aattttattt tggcactcat taatttcctc gtttgcttcc
     3241 ttaataaact cttcgttaga ctgaatttta tgtttaatag attgcttata agtaaccgtc
     3301 tgagcctcaa agctatcaat aacctctgaa ggtaaattta ctgaagtcac atgagaaagg
     3361 gagaaggaag tctcaacatt acccttatct tctttaacag aaagaggtat tttctcttcc
     3421 tgcgttgatt tttcaacaat agaaactact tcttgtgtag gcatatcttg tgaaggttct
     3481 actcgttctt cctcattttc atctaatcta ctgaaatgat ctgtagtcca ttcactagaa
     3541 gaataggtca ttttatcttc caaaggcact tcctctctaa cctcatctcg aggcttctga
     3601 gaaactagct cagcaattat ttgacgagcc atattcgcct ctttcgttac cgttagatct
     3661 tctagttcag ggtctggttc tggttggaat tcaggttgga gttggatttc tggtgttcct
     3721 agtgaggagt tcttttcttt atgtttttct aaaaaatgaa gcaaaccttt ctttttcttt
     3781 ccagcactca taccaaaatt ttccaacaac tgatcaatga tgccaacaaa aggtatttca
     3841 agactttctt cttgtaggat ttcaccttca gaattgagag aataaagtcg atagattcca
     3901 tttattttat tactcttcat tcgttcattg atgatttcta gccttgataa caagtcatct
     3961 cggtactcaa atgtttcctc ttgattagtc agttgattga taattttata catggttcac
     4021 tccttggtta cttgattgtg ctggaaggat tagttccttc atttcctgat aggataagcc
     4081 actttcttct gatagagcaa caaaaagctg cgcttccaaa tctttttctt gatttatgta
     4141 attcttgatt tttttatctg cttcttcttt ttgtcgttgc gctttttcct tgtctttcct
     4201 cactttctca agcttacctt caagttgttc tattttttta ttcattccat tctccttatt
     4261 ggttactact agttgcggtt gttggttcag aagatgtcgg tgttgccgtt gatgtagaag
     4321 aaactggagt tgaacttgaa ctgctagtat ctgcgtaggt tcctacactt tgccctctcg
     4381 cttcagtcaa tacgataggc tctattttat tgatcaatag tttccctgct ttttctacat
     4441 aggtcaaccg aattgtagcc ttagtgctac cagtaagccc cttagaagct ctattatctt
     4501 tctctgttag agtggtacta gtatattgaa cctgagctaa agcaatttta tgttgtttat
     4561 caatgtaaat agatcctgat tcaaaagctt ggtcaaccac atacccctta taggtctggt
     4621 tgactggctt ttgttcttct tgaacagttt cctgatacat agaatccgtc ataaaaggct
     4681 tatagcgtgg tcgattctct tctaaatcct tttttgtata gtaagcaatt aaaaattcct
     4741 cgacctgctg ttcagtaaga gatagttgtt cctgttgttt ctcttctggt ttttgcttcg
     4801 cctcttcctg aacttgacta ttctgtctca aattctgttg tacttgagca atagaatagc
     4861 ccaggataat acataaaacg gcaatgaatc ccacaaagag tccctttacc atttttatta
     4921 acttttgatt atacattctt ttcctttctt atttaggggt aacaaaatag atacctgatt
     4981 gtggtttcaa ggtacgccaa cttgatacat ataaaccacc aaagttcatt tctgaaacta
     5041 agaaactacc atcgctatta acgt
//
LOCUS       DAVKDZ010000117         4944 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000117 DAVKDZ010000000
VERSION     DAVKDZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.119"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(357..1085)
                     /locus_tag="VWZ30_002035"
     CDS             complement(357..1085)
                     /locus_tag="VWZ30_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9335691.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQYYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1243..2653
                     /locus_tag="VWZ30_002036"
                     /pseudo
     CDS             1243..2653
                     /locus_tag="VWZ30_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            2671..3966
                     /locus_tag="VWZ30_002037"
     CDS             2671..3966
                     /locus_tag="VWZ30_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9335692.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYIFAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFTLMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            3971..4279
                     /locus_tag="VWZ30_002038"
     CDS             3971..4279
                     /locus_tag="VWZ30_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9335693.1"
                     /translation="MKQILLVCNVGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTINMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4276..4584
                     /locus_tag="VWZ30_002039"
     CDS             4276..4584
                     /locus_tag="VWZ30_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9335694.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1674 a          829 c          906 g         1535 t
ORIGIN      
        1 gagattgaaa ttcatttcta gcaatttgtt agaagtcaca gtctactatt ctaaccacag
       61 tatactatat gtgttacaag aaaagactca aaaacttctc gtcagtgtaa gaatgtttga
      121 aacactattt aaaactaaat aactataaga agtagatcag gtgaacaatt tcagtaccta
      181 ctctataagc tatacgaaga tatttttcaa catgatggat aaaacgatca ctcttttaaa
      241 gccatcttat ttagaaaaga gaaaccattt aagcatagat gggaccttct tgccctgttt
      301 taaatttttc ttaaactaga gtctgtaaaa gattggcttc attttcacat tttctcctag
      361 gaggaatgtc gtaatgcaaa agaattaaat tcaaataaat catagcgatg acgactaatc
      421 gagtactcaa acggagttcc gttatccaaa taagccactt gttctacttc aaataccggt
      481 tccgttggaa ggagctgcaa gtaatattgc tcattttctg aactagaaga agctcgtaag
      541 atttttgtag cactggcaat atgcaatcct aactttcctt caatgtaatt gtaaatcgat
      601 ttttgtaaaa tatcttcagt aatacctgga ataacatcgg tactcatata agtttgttcc
      661 agaacataag gtttaccgtc aataatacgc aggcggtaaa tattataaac tggactctgc
      721 aaagcgacct gtagtttttc tgctaaaaat tcatttgcaa attctaattt gaagtgaata
      781 atcctcgagt caattgtaga atgagcatct tcagatattt ttgtcagtcc ccggatatct
      841 ctttctggaa cgattaattt tctttttgag ctgccacgag agagaacaaa ggttccctgc
      901 ccacgttttc taaaaattaa gccctcagaa actaacaagt ctaaagcttt tttgatcgtc
      961 attcgactac aggcaaactg tttagctaat gtcatttcat caggcaattg ttcgttcatg
     1021 gtatatttat tacaaagaat cttttcacga atctcattgt aaataaataa atattttgta
     1081 ctcatttaat ctggttctct ctataatatt ctaatagtag tataacaaag ttcaagaaat
     1141 gaggcaaaca caagatatgt atcaacaatt tttaaaaatg tctagacaaa ttttaaaatt
     1201 atgctatact ttatgtatag gataatagaa aggaggttga gcatgaaaaa aaagttagta
     1261 tttcctaatc tgttttggtg gggagctgct tctagcggac ctcagacaga aggtcaatat
     1321 ggaaaagtac atgaaaatgt gatggactac tggttcaaaa cgcatccaga agattttttc
     1381 gataatgtcg gacctcttgt agccagtaac ttttttcata cttacaccga agatttccac
     1441 ttgatgaagg aaattggagt taattctttc cgcacttcca tccaatggag tcgactcatc
     1501 aagaatttag agacaggtga gcctgatcca aaaagtattg ctttctacaa tgccatcatt
     1561 gaagaagcta aaaagaacca gatggatctt gtgatgaatt tacatcattt tgatttacca
     1621 gtggaacttc ttcaaaaata cggtggttgg gaaagcaaac atgtagtgga gttattcgtg
     1681 aagtttgcca agactgcttt cacatgcttt ggagataagg ttcattactg gacaactttc
     1741 aatgagccaa tggtcattcc agaagcagga tacttatatg ctttccatta tccaaatcta
     1801 aaaggaaagg gaaaagaggc cgtacaagtc atctataatc taaaccttgc tagtgcaaaa
     1861 gtgattcaac tatatcgctc attaggactt gatggaaaga ttgggattat tttaaacttg
     1921 acacctgctt atccaagaag taattctcca gaagacttag aagcaagtcg atttacagaa
     1981 gacttcttta acaaagtctt cttgaatcca gctattaaaa ggaactttcc cagaaagatt
     2041 ggtaaaacag ctagagagag atggcgtgtt atggagtcat accgaaaaag agcttcaact
     2101 gatgaaatca aatacggttg attttcttgg agtaaactac taccatccaa aacgtgttca
     2161 agcacaagca aatcctgagg aatatcagac gccctggatg ccagaccaat acttcaaaga
     2221 gtatgaatgg ccggagcgtc gcatgaatcc atatcgtggt tgggaaattt ttccgaaagc
     2281 catttatgat attgctatga ttgtgaagga agaatatgat aatattccat ggtttatcag
     2341 tgaaaacgga atgggtgttg aaaacgaagc acggtttatc ggtgaaaatg gagttatcga
     2401 tgacgtgtat cgtattgaat tttatgaaga acatttaaga tggctacata aagccattga
     2461 agagggaagt cactgttttg gataccacgc ttggaccgca tttgattgct ggtcttggaa
     2521 taatgcatat aagaatcgtt acggatttat ctccgttgat ttagaaacgc aaaagagaac
     2581 catcaagagc tcaggaagat ggtatcgcaa agtaagtgac aataacggtt ttgaagtaga
     2641 aattgaggag taaagagagg agatattcag atgaaaaact caaaatttat agatcaattt
     2701 gccacctttg ctggtaaact agggaaccaa attcatttaa aaaccctaag agatgcattc
     2761 gtaacagtaa tgccattata tatttttgca ggtttaatcg ttcttttgaa caacacggta
     2821 tttaagtgga ttttccaagg ggatacatta acaagattcc aatattgggg aataacaatt
     2881 gcaaacggta ctttaagtat ttcaggtatg attattgctg taatggttgg ttatttctta
     2941 gctaaaaaca gagatttcga aaacccgtta gcagcatcaa tgctatcatt agtttcttta
     3001 attgtgatga tgccaaatac agtttctgta gttcctgacg gagcaaaaga tgcggtaaac
     3061 atttcaggtg ttctttcatt caacaacaca ggtacaggcg caatgttcgc cggggttatc
     3121 gtagcgatta ttgcaacaga attattcatt gaattatcaa acgttaaagc tttacaaatg
     3181 aaccttggtg aaaatattcc accagctgtt agtagatcat ttagcgtatt acttccagtc
     3241 atgaccgtta tctccttatt tggggttgtt tcagcattat tattcaatat aactggaatg
     3301 aacttaatct caatcattac aatctttatt caagaaccaa ttcgtcatat tggtacaagc
     3361 ttaatcgggg tcattattat ttactcttta ggaaatatgt tatggctatt tggtattcac
     3421 caagcagtta tttacagtgc catcctagaa ccattactat taattaacat tactgaaaac
     3481 atcactgcag caaataatgg acaagccatt ccacacatca tcaacctatc acaaatacaa
     3541 acattcactt taatgggtgg tagtggatct acattatgtt tattaatagc aacattctta
     3601 gtgagtcgca atgctgtctc taaaaacgtg gctaaattat cttttggacc tggtatcttc
     3661 aatatcaatg aaccagtatt attcggttac ccaatcgttt ataacatttc attagctatt
     3721 ccatttatca cagttccagt ccttggtatt ttaatcagct acttagcaac agttacagga
     3781 ttcatgagtc ctgcatttat acaagttcct tggactacac cagtattctt aaatgcatgg
     3841 ttagcaacag caggggacgt gagagcagtt ctagttcaat tcatcatctt tgcacttgga
     3901 gttcttctat acattccatt tatcaaagtt aatgacaaag ttgttgaaca agaaatggaa
     3961 ggttaagatt atgaaacaaa ttttattagt atgtaatgtg ggaatgtcga caagtatgct
     4021 tgttaaaaaa atgcaacaaa gtgcgactga acgtgggatt gaaatttcta tccaagcaaa
     4081 atcaatgaca gaagctaaaa agaatatcca tgaagcagat gtcattctaa ttgggccaca
     4141 aattcgttat gagttacttg ctgtgaaaga aattgcagga aatatccctg tagataccat
     4201 taatatgcgg gattacggaa tgatgaatgg agcaaaagtt ctggaacaag cacttgcatg
     4261 gataggagaa ataagatgac agaagaaatg gaatatatct gttttcagct cattgccaat
     4321 agtggagcag ccaaatcatc ctttattgaa gccatccaac tagctaaggc aggaaattta
     4381 aaagaagcga aaattaaagt tgaagaagcg gaagactcct tagtagaagc gcacaagatt
     4441 cactctaatc tcatacaaaa agaagctact ggagaaaaaa taggattctc cttgctattt
     4501 atgcatgcag aagaccaaat ggcttcaacg gagattattc agttgctatc taaagaattc
     4561 atcgatctct atcaaaataa ataggaacac tgtcgttccc atctgtcttt attaatgacg
     4621 gctacattat aggaacctcc aaaagttgga caaaatgatt gaaaggattg gatcctgcat
     4681 tacacaagat ccaatccttt tcttttactc tagtttttat aatatgttga atctagaata
     4741 gtccactgta gtttctaaaa aattgctgga aatggatgtt aagctccaat ttatttgttt
     4801 atatcttatt tcagtttact atactagctt attattctag tgaaaaattg aatgagccac
     4861 tatttattta atatgtatga tgagggaatg actcaaaaac ttcttgtcga tataagaata
     4921 tctgagatag tattttaaaa cgaa
//
LOCUS       DAVKDZ010000118         4835 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000118 DAVKDZ010000000
VERSION     DAVKDZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.120"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            1..597
                     /locus_tag="VWZ30_002040"
                     /pseudo
     CDS             1..597
                     /locus_tag="VWZ30_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            697..906
                     /locus_tag="VWZ30_002041"
                     /pseudo
     CDS             697..906
                     /locus_tag="VWZ30_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            806..1255
                     /locus_tag="VWZ30_002042"
     CDS             806..1255
                     /locus_tag="VWZ30_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9335695.1"
                     /translation="MRKHGLISKSTSKRYYQVAIPFTRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSSLQRANERLKKPIPKHLSPHIFRHTTISILSE
                     NKIPLKTITDRVGHSDSEVTTSIYTYVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1393..2697)
                     /gene="eno"
                     /locus_tag="VWZ30_002043"
     CDS             complement(1393..2697)
                     /gene="eno"
                     /locus_tag="VWZ30_002043"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU9335696.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2861..3307
                     /locus_tag="VWZ30_002044"
     CDS             2861..3307
                     /locus_tag="VWZ30_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEU9335697.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(3304..4419)
                     /locus_tag="VWZ30_002045"
     CDS             complement(3304..4419)
                     /locus_tag="VWZ30_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU9335698.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVEYLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGSGGGLAGGLCAFAQASIVSGIDTCLDLINFNKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(4428..4643)
                     /locus_tag="VWZ30_002046"
                     /pseudo
     CDS             complement(4428..4643)
                     /locus_tag="VWZ30_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084485.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
     gene            complement(4676..>4835)
                     /locus_tag="VWZ30_002047"
     CDS             complement(4676..>4835)
                     /locus_tag="VWZ30_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU9335699.1"
                     /translation="KSEMFYGYEKSFRSLENLEQAIVDYIDYYNNKRIKVKLKGLSPV
                     QYRTKSFE"
BASE COUNT         1516 a         1011 c          861 g         1447 t
ORIGIN      
        1 cgaacaggta gtataacaga agcatcacac gttttccaaa tctcacgtaa taccatttat
       61 ggctggttaa agctaaaaga gaaaacagga gagctaaacc accaagtaaa aggaacaaaa
      121 ccaagaaaaa ttgatagaga tagacttaaa aactatctta ctgacaatcc agacacttat
      181 ttgactgaaa tagcttctga atttggctgt catccaacta ccatccacta tgcgctcaaa
      241 gttatgggct acactcgaaa aaaaagaacc acacctacta tgaacaagac ccagaaaaag
      301 tagccttatt tcttaagaat tttaatagtt taaagcacct agcacctgtt tagattgacg
      361 aaacaggatt cgatacttat ttttatcgag aatatggtcg ctcattaaaa gatcagttaa
      421 taagaggcaa agtatctgga agaagatatc agaggatttc tttggttgca ggggctaaca
      481 aatggtgaat taatcgttcc aatgacttac gaagagacga tgacgagcga cttttttgaa
      541 gcttggtttc agaattttct cttaccaaca ttaaccacac catcggttat tatggtaaaa
      601 tgaaataaga ataggacaaa tcgatcagga tagtcaaatc gatttctaac aatgttttag
      661 aagtagaggt gtactattct agtttcaatc cactatatta tggataatgc aagattccat
      721 agaatgggga agctagaact cttgtgtgaa gagtttgggc ataaactttt acctcttcct
      781 ccctactcac ctgagtacaa tcctattgag aaaacatggg ctcatatcaa aaagcacctc
      841 aaaaaggtat taccaagttg caataccttt tacgaggctc ttttgtcttg ttcttgtttc
      901 aattgactat ataaattgtc taagcgaaac aaccgataag aattggcaca aaagcgaccg
      961 tatttttgtt accaatacag gaaaaccagt tcatagttcc atcttgagca agtcgtctct
     1021 ccagcgagca aacgaacgcc ttaaaaaacc aattcccaaa catctgtccc ctcacatctt
     1081 cagacacacc actattagca tcttatcaga aaataaaatt cctttaaaaa caatcacgga
     1141 cagggttggt cattccgact ctgaagtcac tacttccatc tacacctacg tcacaaagaa
     1201 catgaaagat gaagcaatca atgtactgga taaagttatg aaaaagattt tttaaaaagt
     1261 tttgcccctt ttttgtcctc taaatacaaa aatagccctt cggataaaat ccgaggggct
     1321 agaaacgttg ttaaatcaac gaccgaactt ttgagtttca aggttcggga taaaatagtt
     1381 cactgaacta ttttattttt taaggttgta gaatgatttc aatccacgat attcagctac
     1441 ttcaccaagt tggtcttcga tacgaagcaa ttggttgtat ttagcgatgc ggtctgtacg
     1501 tgaaagtgaa ccagtcttga tttgtcctgc gttagttgca actgcgatat cagcgattgt
     1561 tgaatcttca gtttcacctg aacggtgtga tacaacagca gtgtaaccag cttctttagc
     1621 catttcgata gcttcaaaag tttcagtaag agtaccgatt tggttaactt tgataaggat
     1681 tgagttagca gcaccttctt ggataccacg tgcaaggtag tcagtgtttg ttacgaagaa
     1741 gtcgtcacca acaagttgta ctttcttacc aagacgttca gtaagagctt tccaaccatc
     1801 ccagtcgttt tcatccatac catcttcaat agtgatgatt gggtatttat taaccaattc
     1861 ttcaaggtag tcgatttgtt ctgcagatgt acgaacagca gcaccttcac cttcaaattt
     1921 agtgtagtcg taaactttac gttctttatc gtagaattct gatgaagcac agtcaaatcc
     1981 gataaatacg tctttacctg gtacatatcc agcagcttca atcgcagcaa ggatagtttc
     2041 aacaccatct tcagttcctt cgaaacgagg agcgaatcca ccttcgtcac ctacggcagt
     2101 ttccaaacca cgtgatttaa ggattttctt aagagcgtgg aagatttcag caccgtaacg
     2161 aagggcttct ttaaatgttg gcgcaccaac tggcaagatc atgaactctt ggaaagcgat
     2221 tggagcgtca gagtgagaac caccgttgat gatgttcatc attggagttg gaagaacttt
     2281 agtgttgaat ccaccaaggt agctgtaaag tgggatttca aggtagtcag cagcagcacg
     2341 agctacagcg atagacacac cgaggattgc attcgcaccc aatttacctt tgttaggagt
     2401 accgtcaagt gcgatcatag cacggtcaat agcttgttga tcacgtacat cgtagccaat
     2461 gatagcttca gcaatgatgt tgtttacgtt gtcaacagct ttttgtgtac caagaccacc
     2521 gtaacgagat ttgtcaccgt cgcgaagttc aactgcttcg tgttcaccag tagaagctcc
     2581 tgatggaacc ataccacgtc cgaaagcacc tgattcagtg taaacttcta cttcaagtgt
     2641 tgggttaccg cgtgagtcta ggacttcgcg agcgtaaaca tcagtaataa ttgacatttt
     2701 ttactctcct tatgagttaa attttttaca cctctataat accttaaaac ccctcctttt
     2761 tcaagaaaaa acgttatctt tgtgcaactt ttccttaact ttataaagta atcgctttct
     2821 tttgtctgtt ttattctaac ttttatgata tactgttttc atgacagatt tatcaaaaca
     2881 attacttgaa aaagctcatg gtgggttaaa aataaatccg gatgagcaaa gacgctatct
     2941 tggtactttt gaggaaagag ttcttggata tgtagatatt gacacagcaa atagccctca
     3001 gttagaaaaa ggctttttat ttattttaga aaaccttcag gaaaaagcag agccactatt
     3061 tgtgaagatt tcaccaacta tcgaatttga taagcaagtt ttctacttaa aagaagcaaa
     3121 agaaactgat agtcaagcca ccatagtatc tgaagagcat attacttctc cttttggcct
     3181 ggttattcat agcaatgcac cagttcaagt agaagaaaaa gaccttcgac ttgcttttcc
     3241 aaaactttgg gaagttaaaa aggaagaacc agccaaaaca tccttatgga agaaatggtt
     3301 tagctaaatc ttgcacatat ttaataagtg cccaatattg gcagccgtgc gctccagata
     3361 gagactggca tttttcaaac tatcttctaa aggttcactt ttctccaaaa tagaaaagac
     3421 agcttggata ttttcaaatg gtaggggagg taaatcttca gcaagactac cgcaaatagc
     3481 aataacagga actccaacag gggttctttt tgcaacacct ataggcgctt tcccagcaaa
     3541 gctttgacta tcaagccttc cttctccaac aacaaccaag tcagcatctg aaactttctt
     3601 attaaagttg attaagtcca agcaggtatc aattccagac actatacttg cctgagcaaa
     3661 ggcacacaaa ccaccagcaa ggcctccacc tgatcctgct cctttaattt ctaatgttgc
     3721 aggtgagaat ttttcataaa aatcttggat cgcctgatct acgactgcaa acatagtcgg
     3781 atgtagacct ttttgattac caaaagtgta agtcgcacct tgatgaccac ataagggact
     3841 cacgacatct gctagaatat gaatttgaac accttcagga attttatagc aattttctgt
     3901 tgaaacagaa gctaagttta ataaggattg accggaagca ggcaagacat ttccatccct
     3961 atcataaaat tgataaccta aaccagcagc aatccccagt cctccatcat tactggccgt
     4021 gccaccaaca ccgatataaa tatctttaat ccctttagaa atgagatgga gaatcaactc
     4081 tccaatacca caagtttgga tttgaagtgg atttcgcttc tctagcggaa tttttccaag
     4141 accaaccaag tcagctactt caaatagtgc cagttcccct ttttgaaaat agcgcatggc
     4201 ttctttttgt ccaaaagggt ctgtcacttg gatccatttt tcttttaggt caagagaatg
     4261 tcggatagca tctacagtac cttctccccc atcaccaaca gggcagagga gatattctac
     4321 atctgctatc gattgttgga agcctctttt tattgcttca gctacctgtt gagctgtcaa
     4381 gctttcctta aacgaatccg gtgcaattac aatcttcata ttttccctca ttctaaacgg
     4441 tcaatcaaag gaagaacttc taaaaaatcc ctcttgtcaa catgatgtgg tatttctttt
     4501 ttgagcactt ctttggcaca aaaggcgatt cctaactccg ccgacttcaa cattaataga
     4561 ttattaaccc catcaccgat tgccaccgtt ctttctttag aaagttttag tttctttctc
     4621 catttttcca gagtctcttt tttgtgtact gacccccaaa agttagacaa ttaatttatt
     4681 caaaggattt agttctgtat tgtacagggc taagtccttt tagttttacc ttaattcgtt
     4741 tattgttgta gtaatcaata tagtctacaa tagcttgttc aaggttttct aaagatctaa
     4801 agctcttctc ataaccataa aacatctccg atttc
//
LOCUS       DAVKDZ010000119         4764 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000119 DAVKDZ010000000
VERSION     DAVKDZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.121"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            116..1127
                     /locus_tag="VWZ30_002048"
                     /pseudo
     CDS             116..1127
                     /locus_tag="VWZ30_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(1417..1638)
                     /locus_tag="VWZ30_002049"
     CDS             complement(1417..1638)
                     /locus_tag="VWZ30_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335700.1"
                     /translation="MDLFQELICLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            1603..2232
                     /locus_tag="VWZ30_002050"
     CDS             1603..2232
                     /locus_tag="VWZ30_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335701.1"
                     /translation="MSKANQLLEQIHEEGIRKSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANGDNTSAENQP
                     NATAESNIETGSDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            complement(2268..3491)
                     /gene="pepT"
                     /locus_tag="VWZ30_002051"
     CDS             complement(2268..3491)
                     /gene="pepT"
                     /locus_tag="VWZ30_002051"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU9335702.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            3646..4740
                     /gene="hemH"
                     /locus_tag="VWZ30_002052"
     CDS             3646..4740
                     /gene="hemH"
                     /locus_tag="VWZ30_002052"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU9335703.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEDRLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILEEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTIRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
BASE COUNT         1605 a          864 c          941 g         1354 t
ORIGIN      
        1 gacatacaac ttaaaaagag gtggaatgtt tactagttaa ttaattggtt aattaattta
       61 tttaatcatt taataatgaa gacatcatat aaataattat agaaatgagg taattatgaa
      121 atttaaaaag aaatatatag cagctggatc tgttgttatc ctttccttaa gtctgtgtgt
      181 ttatgctctg aaccaacata gctaacaggc caatacagat aaaaatcgtg tttcatatgt
      241 aaacagtaat aaagacacta agaagactga aaatttgact ccagactagg ttagtcaaaa
      301 agaaggaatt caagcaaaac aaattgttat taagattaca gatcaaggtt atgtaacttc
      361 tcatggcgat cattatcact actataatgg taaagttcct tttgatgcgc tctttagtga
      421 agagctatta atgaaagccc ccaactatca gccgaaagat caagatatcg tcagttaggt
      481 taagggagga tatattatta aggtagatgg aaagtattat gtttacctta aagatcaagc
      541 tcatgcagaa aatgtacgaa caaaagatga aatcaatcgc caaaaacaag aacatggtaa
      601 agacgataaa ggagctagtg cagaagtatc tgtagcaaaa ttacagggac gttatactac
      661 cgatgatggt tatatatagt gagttgaatt tagaatagca catcatagtt tctaaatcat
      721 tgttagaaat tactttaaaa tccccttatc aatttgctca tattctattt caacctacta
      781 tagtaccaca tggtggtcac tatcatttca ttcctgagag tgacttgtcg gcaggtgaat
      841 tagcagccac atatgtcttt aaccctaatg atattgtcag agatacaggt gatgcttata
      901 tagtaagaca tggggatcac tatcactata ttcctaagtc atctcttaac aatcccccat
      961 cacactctaa cacggaagag gtaggaagtt cttctagttc tatattaagt aatccgtcac
     1021 tacatgtcca ccatgaggaa gaagatggtc atggttttga tgccaatcga attataagtg
     1081 aagattcaga aggttttgtg attcctcatg gcgaccacaa tcactacatc aaggttcaaa
     1141 ctaagggata tgaagcggct ttgaaaaaca agattccatc cctgcaatcc aactatcaac
     1201 ctggaacttt tgatgagaag gcagtcttgg caaaagtaga tcaacttcta gctgatagca
     1261 gaagcatcta caaagacaag cctatcgaac aaagacagat tgagttagct ttaggtcagt
     1321 ttactgaaag tttgaaaaaa ataaaagtta gttgattgat tacttctcta aattttgggc
     1381 acactaaaaa gaggtcttga cacctaaact ttaaaattaa agcatattga atccgtgctg
     1441 ctgtgcgaag cgaatagcgt cttggacgaa cattgtctta tctggatttc ttatttctgt
     1501 aaggccttgt aatcttggat tataactctc ctgttttagg agtacatggt agatggcaac
     1561 caataatctg cgacagatag caaggaagaa gtagaaagtc ttttgagcaa ggcaaatcaa
     1621 ctcttggaac aaatccatga agaaggaatc agaaaatcct tggcagaaga agtagaaaat
     1681 ctaaaagctg ccacaaacaa ggctgatgca gacttggatg aagtaaacag tcaggtaaaa
     1741 gatgtcttga ctcgtatcgc tagcgccctt caacaagaaa aggaaaatgc tgagcaagat
     1801 cctcagacac ttgtactcta tcaaaaactc tacgatattc tcatgtcgct tcactcttat
     1861 ttagaaagta ataatggatc tgatgcggac tttgataagg tggatgcatt attagatcaa
     1921 ctttctgcta agagtaaaga caaaacagct ttacttgaat cgacaaaagc tattctagtt
     1981 ttgaatcatc aaatccagtc aaaagctagc gcaagtgaag aaacaagtcc agcaagaaat
     2041 gctgaagcaa atggtgataa caccagtgct gagaaccaac caaacgcaac tgcagaatct
     2101 aacattgaaa ctggtagcga cgaaaacaaa ccaagcaata caagagattc taaacctgct
     2161 gaatcaactt cagaaaatga aacaacagaa tcttctacaa ctactggaaa tcaagaaaaa
     2221 ccagtagaat aaaaagaaag gcgaagtagc tgagccacct cgtcttttta gcctttataa
     2281 gctacgatgc caatgatggt atcaactgca cgttccatag tctgaaggct aacgtattca
     2341 aaacgtccgt gcatattttc gccacctgca aagatattcg gagttgggat tcccataaag
     2401 gaaatcttag agccgtctgt tccaccccgg attggttcga taataggcgt gatacctaga
     2461 tcttccataa cggctttagc aatggtaatt ggagtcatat ctttttcaat gacttctttc
     2521 atattgtagt actggtctgt caagttgaga gtgacacggt cgctcccaag ttcttcattc
     2581 atcttatcag cgatagattg catggatgct ttacgcgctt caaaggcatc tttttcaaaa
     2641 tcacgaatga tgtagcttgc acgcgcctcc tcaacactac ctgtcacatc cattagatgg
     2701 taaaaacctt ggtaaccttc agttaactca ggtcggtcat tttctggaag ttgattatga
     2761 aaatcaattg ctagctgaag ggcattgacc atctgccctt tggcagtacc agggtggaca
     2821 ttacggcctt ggaaatgcaa ttcagcacca gcggctgaga aagtctcgta ctgaagttca
     2881 cctagtggac caccatcaac agtgtaggca aaatccacat caaaatcttc tgcatcaaat
     2941 ttattggcac caacaccgat ttcttcatct ggaccaaaac caacacgaat ctcacagtgc
     3001 ttaatttcag gatgagcagt tagatattca atggctgtca taatttcagc aattcctgac
     3061 ttgtcatcag cacctagcaa ggttgttcca tctgttgtga tgagcgtttg tcctggatat
     3121 ttttcaagac tcttgaagtc ggctggatcg agtttgaaac cagaattccc tagttcaatc
     3181 acaccaccat cgtagttttc aattacctgt ggattgactc cttcagcatt aaaatcagca
     3241 gtatccatgt gcgatataaa accaatctta cgtgttaaag acggatcgtt ggctggcaag
     3301 gttccaatag caaaaccatt cggtagatag taaacatttt gcagtccaac acgtttcatt
     3361 tcaggaatta ggacatttgt tgcgaagtca acctgactct gtgtacttgg agtagtagta
     3421 gagtgttcat cagagcgcgt gttgacctta acgtaggtta agaagcggtc caagagattt
     3481 ggataagtca taaaaaaact ccttttgaat ttattttttc cattgtatca tagaaagaag
     3541 agaaaaacaa aagacagaag attgagaatg tccctatgtt agcgtttact tatcgatgaa
     3601 ttaatgataa aataaacttg ataaaaacat cacaaggaag cagtcatgaa aaaagcaatt
     3661 ttaatgatga ccttcggttc accagaagag attacctttg aaggtgtagc tgattttttc
     3721 acaaatattc gtcgtggggt gagaccccaa gaccacgaga ttcagactct ctatgataat
     3781 tatgtacgaa ttggaggcac gcctctgcaa aaaattaccc gtcaagaggt tgccttggta
     3841 gaagataggc tggggaatga atacagtgtc tactttgcta ataagttttc cagtcccttt
     3901 attccagatg tgatcggtca aatggaagca gacggaattg aacagtgtat ttgcttgatt
     3961 ttggagcccc attattcttt ctactctgtc atgggctatg aaaaattttt agaaagcaaa
     4021 caaattcaat ttttggtcat taaggactgg tatcaggaag aagcgctctt aaactattgg
     4081 gcagatgaaa ttgctaagat tttagaagaa gaagtaaagc aagacagctt taaagtcatc
     4141 ttttcagccc atagtgtgcc tatttttgcc ttggactttg gtgatcctta tatagaccaa
     4201 attttcgaaa atagcaagct agtcgctgaa aaactgggct tgagttcaga acaatatacc
     4261 aacacttggc agagtgagag tgatattggt attccatgga ttaaaccaga tgttttggag
     4321 tatctcagag aacagacaga acatccagac cattatattt ttgtacctat tagtttcatc
     4381 agtgagcata ttgaggtctt gtttgacaat gatgtggaat gttatgactt atgtcaggaa
     4441 tttggggtaa actaccatcg tccaccaatg ccaaatacag attctcgttt gattgatgct
     4501 ttggtcaata caattagagt caatgaaaat caagagttta aggaatttct cccagaagaa
     4561 gagacctttg atgagttagt tccttcagac gagacgaaaa acattttggc tgaatctgaa
     4621 gatttacaaa tgccagaatt tgtgaaaaaa ctgattgaga aaaaagggcg ggagaatgtt
     4681 aagatgcctt atcttatcaa aaaaatgctt gaaaaagcag gcaagttacc caaagagtaa
     4741 agaaaaaagg atttagcttt gcgc
//
LOCUS       DAVKDZ010000120         4483 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000120 DAVKDZ010000000
VERSION     DAVKDZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.122"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..1593
                     /locus_tag="VWZ30_002053"
     CDS             <1..1593
                     /locus_tag="VWZ30_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEU9335704.1"
                     /translation="LDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAM
                     VTKLLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEP
                     SKLGTMLFEYSKLFMENKEDNSTMDSSNNFSHQLTQSLLKSPNLILRGAPGTGKTYLA
                     KEIAKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQK
                     AKETQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGW
                     SLPSKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFII
                     DEINRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMND
                     IDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQEL
                     NSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTN
                     NEQKDQAVADDNESDENDDADY"
     gene            1580..2896
                     /locus_tag="VWZ30_002054"
     CDS             1580..2896
                     /locus_tag="VWZ30_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEU9335705.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKKQKILETINQEIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKITRRNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEIGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            3071..4057
                     /locus_tag="VWZ30_002055"
     CDS             3071..4057
                     /locus_tag="VWZ30_002055"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEU9335706.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     VGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            4121..4192
                     /locus_tag="VWZ30_002056"
     tRNA            4121..4192
                     /locus_tag="VWZ30_002056"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4154..4156,aa:Arg,seq:ccg)
BASE COUNT         1477 a          733 c          911 g         1362 t
ORIGIN      
        1 ttggatcagc gattttcaaa attaaaatca gatttgtatg aaattatcaa agaaggtatt
       61 cgttttaact ttgaaaatcc tatttttgat atgaaaagat caacaaatga atttattggt
      121 cgttctgcta tggtgacaaa attactttgt atctatactg agggagatcc tttctttggt
      181 gtaaatatta atagtcagaa agaattttgg aaccactttg tttctcagac aaatcaaggt
      241 ggaccttatc tgcaaaatca taaaataatt gaactggtgt ccaaaactta tcctgagttg
      301 gagccatcga aattaggaac tatgcttttt gagtattcta agctttttat ggaaaataag
      361 gaagacaata gtacaatgga ttcatcaaac aattttagtc atcaattaac tcaatctcta
      421 ttaaagtctc caaaccttat ccttcgtggt gctcctggca caggaaagac ttatcttgct
      481 aaagaaattg ccaaagaatt aacggatggc aacgaagacc aaatcggatt tgtacaattt
      541 cacccatcct atgattatac ggattttgta gaaggtttaa gaccagtatc aaatggggat
      601 ggagctattg agtttaggct acaggacggt atttttaaag atttttgtca gaaagcaaaa
      661 gaaacccaat tgattggagg acaagataat tttgatgagg cttgggattc ttacttagaa
      721 tatataaatg ttgctgaaga aaaagaatat ataacaaaaa catcttactt atctgttaat
      781 agtagacaaa atttgtcagt aaattatgat agtggtgttc caggatggtc actacctagc
      841 aaatatgttt acgagttgta taaagataaa aattataata agcaagaata ctacaaaagt
      901 ggtggaaaaa ctgtcctaga aacattgaga aagagatttg gtttgaaaga ctatgtttcc
      961 ccaacagaaa ttgatactga taagaaattc gtcttcatca tcgatgaaat caatcgtggg
     1021 gagatttcta agatttttgg cgaactcttt ttctctatcg accctggcta tcgtggtgaa
     1081 aaaggaagtg tttctaccca atatgcaaat ctacacgaaa ctgatgaaaa gttctatatc
     1141 cccgaaaatg tttacatcat cggaactatg aatgatattg atcgttcagt ggataccttt
     1201 gattttgcta tgcgtcgtcg ttttcgtttt gttgaagtta ctgccgaggg tcaagttggc
     1261 atgttggata aagagctgaa tatccatgca gaagaagcaa aaattcgtct aagaaacttg
     1321 aacgctgcta tcgaaaatgt tcaggaatta aacagtcatt atcatattgg accaagttat
     1381 tttcttaagt tgaaggatgt agattttgac tatgaattac tctggtctga ttatattaag
     1441 cctctcctag aagactactt gcgaggttct tatgatgagg ttgaaacttt ggaaactttg
     1501 aaaaaagcat ttgaactgac aaataatgag caaaaagatc aggcagtagc tgatgacaat
     1561 gaaagcgatg aaaacgatga tgcggattac tgataatcaa cacaagatta ttaaagaaaa
     1621 atttgttgaa gaatatccta aactaagcaa tcttctttta gacagaacct tggaaagtct
     1681 atcccaagat gaacgtattt tcatttttcc aaatgatttg actcatactc cagatttgga
     1741 taagaaacaa aagattttgg aaacgatcaa tcaggaaatc aagacaggaa atgtgattgg
     1801 ttttcttgga tatggtcagg aaagattaac gatttcctca cgattttctg atgagagtaa
     1861 tgaccatttt ttgcattatc tcttaaacaa ggttcttcat atcaatctca ctagtttaga
     1921 tgttgctttg tctcgtgaag agaggcttta tcaacttttg atgtatctct tccccaagta
     1981 tctacaagct gctattcgaa aaggtcttta taaggaatat catcgatttt ctcataacga
     2041 cagtcatgtt aagggagtga ttgatgtaag aaaccatctc aagaaaaatc ttcctttcac
     2101 gggaaatatt gcctacacaa cgagagagtt cacctatgat aatcccctca tgcagttggt
     2161 ccgtcacact attgaataca ttaagaatca gaaaagcatt ggtcaagggg tactagataa
     2221 tctctcaact agtcgtgaaa acgtatctga aatcgtgcgt gtaacgccct cttataaact
     2281 agctgatcgt gctaagatta ctcggagaaa tcaatctaaa cctatacgtc atgcatactt
     2341 tcacgagtac agaaacttac aagaactttg tctgatgatc ctaaaccaag aaaagcacgg
     2401 tttaggctat caagatcaaa aaatctatgg tattctcttt gatgttgcct ggctttggga
     2461 agagtatgtt tacaccttgt tgccaaaagg ttttgtacat cccagaaata aggataagac
     2521 ggatggaatt tcagtatttt ctgttgggaa acgaaaagta tatccagatt tttatgacag
     2581 agaacgaaag attgttctag atgcaaaata taaaaaactg gaattgactg aaaaaggaat
     2641 caaccgtgag gacttattcc agctgatttc ctattcttat attttaaaag ctgagaaggc
     2701 tggacttgtt tttccgagca aggacaaggt tattgataat gagataggaa acctagcagg
     2761 ttatggagct ttgttgaaga aatggtctat ccaaattcct cagaagtctt catcttatag
     2821 tgcattttgt aaaatgatgg aaaattcaga agaaaatttt aaagcgatta ttgatgaaga
     2881 agtggggaga aaataatttc ccctctttct tttattttta acacaagcaa aatccgaaca
     2941 atatatttta attcataaaa taatttgaca aaaactgtac tttggtttat aataaattaa
     3001 ggaaatgtcg gaaaaggcgt agtgatgcga gagcataggt aggtcattac aaaagaaacg
     3061 agacatcgat atgttaaatg aatttccaat ttttgattac gaagatattc aattgattcc
     3121 aaataaatgt gtcattaaaa gccgtgcaga agcggataca agtgtcactt taggaaatca
     3181 cacctttaaa ctacctgttg tgccagcgaa tatgcagacg attttggatg aaaatgtagc
     3241 agagcaactg gctaaaggtg gttacttcta cattatgcac cgttttgatg aggcaggacg
     3301 cattcctttt attaaacgaa tgcacgatca agggctcatt gcttccatat ctgtcggtgt
     3361 taaggactat gagtatgatt tcgttagaca gctcaagact gatgctcctg agtatatcac
     3421 tattgatatt gctcatggtc atgcggatag cgtgatttct atgattcaac acatcaagaa
     3481 agaattgcca gatacctttg tcattgctgg aaatgtggga acaccagaag ctgtgcgtga
     3541 attggaaaat gttggtgcag atgctactaa ggtcggaatc ggtcctggta aggtttgtat
     3601 caccaaggtt aagactggtt ttggtacagg tggttggcag ttggctgctc tacgctggtg
     3661 tgccaaggct gcgcgtaaac cgattatcgc tgatggagga attcgtactc acggtgatat
     3721 tgccaagtct atccgcttcg gtgctagcat gatcatgatt ggttccctct ttgcaggaca
     3781 tattgaaagt ccagggaaaa cgattgaagt cgatggtgaa cagttcaaag aatattatgg
     3841 ttcagcctca caatatcaaa aaggtgctta caaaaatgtg gaaggcaaac gtatcttgct
     3901 tcctgctaaa ggtcatctgc aagacacttt aactgagatg gaacaagacc ttcaaagtgc
     3961 tatttcttat gcaggtggac gtcaggttgc tgaccttaaa cacgttgatt atgtgatcgt
     4021 gaaaaactcc atctggaatg gggatgcttc ccactaagac ggggtctttg accaaaaaac
     4081 ttgttattag agcaaatttc tgttataata aaacaagttt ccacccttag tgtaatggat
     4141 atcacgtaag attccggttc ttgagatggg ggttcgattc cctcagggtg gatgtaaaca
     4201 tcctaaaaag cctttaatag ggcttttttc tttacactgc tttaaatttg tccctaatac
     4261 tcaatgaaaa tcaaagagca aactaggaag ctagccgtag gctgctcaaa gcactgcttt
     4321 gaggttgtag ataagactga cgaagtcagt tacatatacc tacggcaagg cgaagctgac
     4381 gtgatttgaa gagattttcg aagagtataa aactgaaaat ctctgtctaa agaatttcca
     4441 aatcccctcc agtcccgttt taaagtgact cagggggctt ttt
//
LOCUS       DAVKDZ010000121         4429 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000121 DAVKDZ010000000
VERSION     DAVKDZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.123"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            175..813
                     /locus_tag="VWZ30_002057"
     CDS             175..813
                     /locus_tag="VWZ30_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU9335707.1"
                     /translation="MLNKMKVSFLLGLGGLAVMSFVVMMGYTIGSQSVTHHMQKQIQT
                     EAHKLLTQEKEKEKASVLSDELVKEFLTQYYTKGKLGENNNRIKLYMTDSAYKEEVAR
                     QEDSINQVYKDYMLDYRFETAQIYVNTEANEALAEVSYQVVYVSDVSDKAQKSTQTET
                     KSLKLSYTKMSDKFLVKQVTVWNGKLENLKEVSDGADSSIPDIQGTTTSETN"
     gene            852..1940
                     /locus_tag="VWZ30_002058"
     CDS             852..1940
                     /locus_tag="VWZ30_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEU9335708.1"
                     /translation="MTRIEAVKQKAILEVAESLGYSFKRLSGQVYEHPEHDSFRIFAD
                     TNTFKWFSRDIQGDVIDFVQLITGVTFKEALSYLETGDFEQTKMVEETYQPFRYYLRE
                     EPFYQARIYLNDIRGLSDDTINAFGRQGLLAQAHYQTKIFQESVLVFKSYSHHGQLEG
                     ASLQGLVKNNERHDRDYLKKIMKGSHGYVGMSFDIGKPERLIFCESVIDMMSYYQLHQ
                     NQLSDVRLVSMEGLKLSVIAYQSLRLVAEEQGKLEFLDTVKPSRLTHYLHAIQETTTF
                     FQTHPSLITLAIDNDGAGRDFCQKLSEKSLPIETDLPPLQELETKADWNDVVKSQKDL
                     SLKDMIQSAKLQVIRSNPPPRKNTALEL"
     gene            1994..2227
                     /locus_tag="VWZ30_002059"
     CDS             1994..2227
                     /locus_tag="VWZ30_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335709.1"
                     /translation="MSVIERLAERVARQEEKVAKETEKLGTYREQLQAAMYSIFVKRQ
                     QSSHLTFDEALNQAFGKQENHSQVIENRNEETE"
     gene            2224..2619
                     /locus_tag="VWZ30_002060"
     CDS             2224..2619
                     /locus_tag="VWZ30_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEU9335710.1"
                     /translation="MTKDWTFDQSLDDNTPTSSTEERAKIAALFHKEEKEETGEVNYV
                     AAFEMEQQKAKEKSVSKPKVQEQSTPKEVPSSTITSDYKQHLANVMAQNNEDILQSQK
                     KIEELNQLIDDKNKQNKKLQAISEAIDDL"
     gene            2665..2952
                     /locus_tag="VWZ30_002061"
     CDS             2665..2952
                     /locus_tag="VWZ30_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335711.1"
                     /translation="MRLFKKQTHNQDSFKRLIHRLSGMSETDLHKVNQLLDVVFDATS
                     QTIHEETMTPKDMPKEPILDETITEAKNKLNTEQLEKRIEQFRQAKKPKSG"
     gene            3021..3497
                     /locus_tag="VWZ30_002062"
     CDS             3021..3497
                     /locus_tag="VWZ30_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9335712.1"
                     /translation="MIGENIKSLRRTHDLTQPEFAKIVGISRNSLSRYENGTSSVSTE
                     LIDRICQKFNVSYVDIVGEEKMLTPVEDYQLTLKIEVIKERGAAVLSQLYRYQDSQGI
                     AFDDETNPWILMSDDLSDLINTKIYLVDTFDEVERYNGYLDGIERMLELARHQVVA"
     gene            3497..4279
                     /locus_tag="VWZ30_002063"
     CDS             3497..4279
                     /locus_tag="VWZ30_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEU9335713.1"
                     /translation="MALEAFRQDDFDLALKRTIRSLTRGKTVSTSPQAILLGGQSGAG
                     KTTIHWIKQKEFQGNIIIIDGDSYRSLHPNYLVLQEEYGKDSVDYTKFFAGKMVEHLV
                     DELSKDGYHLLIEGTLRTTEVPRKTAQLLKSRDYQVSLALIATKPELSYLSTLIRYEE
                     VYAENPSQARATSKEHHDGIVEHLVDNLRELETDKLFDQIQIYQRDKRCVYDSDTDDG
                     SVADVLQDCLFGKWSKVEEEMIKEAKEYLKRLSKSNNENILV"
BASE COUNT         1529 a          759 c          940 g         1201 t
ORIGIN      
        1 taaaataggc tccataatat ctatagtgga tttacccact acaaatatta tagagccgct
       61 tttttgtggt cgagaactat tttgtcctag gctcttttct tttttgtgat aacttttcca
      121 agtcaatctt gttatgatgg tgctatctta cggaaaaagg agatggcact attgatgtta
      181 aataaaatga aagtgagttt tttgcttggt ttaggtggtc tagcagtcat gagctttgtg
      241 gtgatgatgg gctacactat tggttctcag tctgtgaccc atcacatgca gaaacaaatc
      301 cagactgaag ctcataaact attgacccaa gagaaagaaa aggagaaagc aagtgtcctc
      361 tcagatgagt tggtcaaaga atttctcacc caatactaca ctaagggaaa gctgggggag
      421 aacaacaacc gtattaagct ctatatgacc gactctgctt ataaggaaga agtagctcgt
      481 caagaagatt ccatcaatca ggtctataag gactatatgt tggattatcg ctttgagact
      541 gctcaaatct atgtcaatac agaagcaaat gaagctcttg cggaagtatc ctatcaggtt
      601 gtttatgtgt cagatgttag tgataaggct cagaaaagta cacaaacaga aacgaaatct
      661 cttaaactat cttataccaa aatgtcagat aaatttcttg tcaagcaggt aactgtttgg
      721 aacggaaagc tagagaactt aaaagaagtt tctgatggag ctgattcaag cattccagat
      781 attcaaggca ctactacaag tgagaccaac tagcaggagt tacccttctg cttttttgat
      841 agggagatga gatgacaaga attgaagcag tgaaacaaaa agcaatctta gaggttgcag
      901 aaagtctagg ttattctttt aaacgactat cagggcaagt ctatgaacat ccagaacacg
      961 attcctttag gatttttgcg gataccaata cttttaaatg gttttcacgt gacatacagg
     1021 gagacgtgat tgattttgtt cagttaataa caggagtgac ttttaaagaa gctctgtctt
     1081 accttgaaac aggggacttt gaacagacta agatggttga agagacttac caaccctttc
     1141 gctattatct aagggaagaa ccattttacc aagcacggat atacttgaat gacattcgtg
     1201 gtttaagcga tgacactatc aatgcttttg gcagacaagg gctgttagcc caagctcatt
     1261 atcagacaaa gatctttcaa gaatcagttc ttgtttttaa gagttacagt catcatgggc
     1321 aactagaggg ggcaagtttg caggggcttg ttaaaaataa tgagagacat gatcgagatt
     1381 atctcaagaa aatcatgaaa ggttctcatg gttatgttgg gatgagtttt gatataggaa
     1441 agcctgaacg ccttatcttt tgtgaatcag tgattgacat gatgagctat tatcaactcc
     1501 atcagaatca gttatcagat gtgcgtttag tttctatgga aggtctaaaa ctctctgtga
     1561 ttgcgtatca gtccttacga ttagtagctg aggaacaagg caaacttgag tttttagata
     1621 ctgtaaaacc aagtcgatta acgcattacc ttcatgccat acaagagaca accacttttt
     1681 ttcagactca cccaagtctg ataaccttag ctattgataa tgatggagca gggcgagact
     1741 tttgtcagaa attatctgag aaaagccttc ccattgaaac agacttacca ccattacagg
     1801 aactggaaac taaagctgat tggaatgatg tcgtgaaaag tcagaaagac ctttctctaa
     1861 aggatatgat ccaatcggcc aagttacaag ttatccgaag caatcctcct cctagaaaaa
     1921 ataccgcttt agagttgtga taacaaaatc aagtttctta gcttaatcta gaaagtgata
     1981 aggaggacac cctatgagtg tgattgaacg cctggctgaa agagtagcta ggcaagagga
     2041 aaaagttgcg aaagaaacgg aaaagctagg aacctatcgt gagcagttac aggcagctat
     2101 gtatagcata tttgtcaagc gtcagcagtc gagtcatctt acttttgatg aggctttaaa
     2161 ccaggcgttt ggtaagcagg aaaatcattc acaggtaatc gaaaatagaa atgaggaaac
     2221 agaatgacaa aagattggac ttttgaccaa tccctagatg ataacacacc aacgtcttcc
     2281 acagaagaac gtgcgaagat tgcagcccta tttcataagg aagagaaaga agaaactgga
     2341 gaagttaatt atgtggcagc ctttgaaatg gagcaacaaa aagctaagga gaaatcagtt
     2401 tcaaaaccaa aagttcaaga gcaatccaca ccaaaagaag tgccctcttc aaccattacc
     2461 agtgactaca agcaacatct agccaatgtg atggctcaaa acaatgagga cattcttcaa
     2521 agtcaaaaga aaatcgaaga gctgaaccag ttgattgatg acaaaaataa gcagaataag
     2581 aaattacaag ccatttcaga agctattgat gacttgtaag tacgagcagt ccttatgtgg
     2641 gctgcttttt acgaggggga acagatgagg ttattcaaaa aacaaacgca caatcaagat
     2701 agttttaagc gactcattca tcgcttatca ggaatgtctg agactgactt acacaaagtt
     2761 aaccaattat tagatgtcgt ttttgatgct acaagtcaaa ctatccatga agaaacaatg
     2821 actcctaaag atatgccaaa ggagcctata ttagatgaga ctattactga agctaagaat
     2881 aaactgaata ctgagcaact ggaaaaacgg atagaacagt ttagacaggc gaagaaaccg
     2941 aaaagcggat aatgcaccaa aaatggtgcg ttatccattt tttgtgctat aatgaaagta
     3001 acgaatagga ggtcttatct atgattggag agaatatcaa atcattacgc agaacacacg
     3061 atttgacaca accagagttt gccaaaattg ttggtatttc aagaaatagc ttgagccgtt
     3121 acgaaaatgg gacaagctct gtttcgacag aactcattga tcgaatttgc caaaaattca
     3181 acgtgtcata tgtcgatatt gtaggagaag aaaagatgtt aacaccagta gaagattatc
     3241 aattgacgct gaaaatagaa gtcatcaaag agcgtggagc agcggtttta tctcaactgt
     3301 atcgctatca agatagtcaa gggattgctt ttgatgatga gactaatcct tggattttga
     3361 tgagtgatga cttgtctgac cttatcaata ccaagattta tctggtcgat acctttgatg
     3421 aggtagaacg ctacaatggt tatttggatg gcattgagcg tatgttggaa cttgctcgtc
     3481 atcaggtggt ggcttaatgg cattagaagc atttcgtcaa gatgactttg acctagccct
     3541 gaaacgcacc atccgttctt taacccgagg caagacggtt tcgactagcc cacaagctat
     3601 tttacttggg ggacaaagcg gagcaggtaa gacgaccata cactggatta aacaaaagga
     3661 atttcaaggc aatatcatca ttattgatgg cgatagctac cgctctttac acccgaatta
     3721 tcttgtctta caagaagaat atggtaaaga tagtgtggat tacaccaaat tctttgcagg
     3781 aaaaatggta gagcacctcg ttgatgaact cagcaaagac ggttatcatt tgttaattga
     3841 aggaaccttg agaacgacag aggttccaag aaaaacagct cagctattga aatcaagaga
     3901 ttatcaagta tcactcgcat tgattgcgac taagcctgaa ttgtcctatc tcagcacctt
     3961 aattcgctac gaggaagtct atgccgagaa tccaagccaa gctagagcaa cttctaaaga
     4021 gcatcacgat ggtattgtag aacatttggt tgataatcta agagagttag aaactgataa
     4081 actctttgat cagattcaaa tttatcaacg agacaaacgc tgtgtctatg attctgatac
     4141 agacgatggt tcagttgctg atgtgttgca agattgtctc ttcggaaaat ggagtaaggt
     4201 tgaggaggag atgattaagg aagctaaaga atatttaaaa cgattgagta aaagtaataa
     4261 tgaaaatatc ttagtatgaa atagaggtag aaaatagtgg aaacagtaaa cacaatttta
     4321 ggaatagtag gatctatatt tggcataatt gctacagcaa tttctttaaa aaaccagaac
     4381 gatattaaaa aaattataaa gaattccaat aatagccaaa atataggta
//
LOCUS       DAVKDZ010000122         4331 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000122 DAVKDZ010000000
VERSION     DAVKDZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.124"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            1..410
                     /locus_tag="VWZ30_002064"
                     /pseudo
     CDS             1..410
                     /locus_tag="VWZ30_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(725..1699)
                     /gene="pta"
                     /locus_tag="VWZ30_002065"
     CDS             complement(725..1699)
                     /gene="pta"
                     /locus_tag="VWZ30_002065"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU9335714.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(1743..2639)
                     /locus_tag="VWZ30_002066"
     CDS             complement(1743..2639)
                     /locus_tag="VWZ30_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9335715.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIYIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            complement(2636..3454)
                     /locus_tag="VWZ30_002067"
     CDS             complement(2636..3454)
                     /locus_tag="VWZ30_002067"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU9335716.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINCVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            complement(3438..4109)
                     /locus_tag="VWZ30_002068"
     CDS             complement(3438..4109)
                     /locus_tag="VWZ30_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU9335717.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGVKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            4237..>4331
                     /locus_tag="VWZ30_002069"
     CDS             4237..>4331
                     /locus_tag="VWZ30_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase"
                     /protein_id="HEU9335718.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVL"
BASE COUNT         1324 a          945 c          770 g         1292 t
ORIGIN      
        1 cgattttatc gtcctacttt tcgtatgcac ttaaccaata aagagatttt aaacaatctt
       61 ttgagttatt cggaaggctt gaaacaccac tatcagctct tcagctcttg ctttttcact
      121 tccagaataa ggaaccggag aaatttttcg gattcattga ggacaatctt aagcaggttc
      181 atcctatttt tcagactgtc tttaaaacct tcctcaaaga taaagaaaag attatcaacg
      241 cccttcaact acactattct aatgccaaac tggaagcgac caataatctc atcaaactta
      301 tcaagcgcaa tgcctttggt tttcggaact ttgaaaactt caaaaaacgg atttttatcg
      361 ctctgaatat caaaaaagaa aggacaaaat ttgtcctttc tcgagcttag ctgacttcaa
      421 cccactacag ttgacaaaga gccagaaaac gcataatagc aggtgtcaaa gcactcaata
      481 ttatgcgttt tctcatgaaa aaattctctt ttcatactca tttaaaatca aaaagggaat
      541 ttatcgtttt acggacatgt atattacaga tttctatagg tcgtgtatag acaggatatc
      601 atcgcttgga agttttcctt tttgacacca atcagtaatt tagttctcag gaattaatgg
      661 cagtgctttt cgtacatagt ttcataacta cagtatagca tagtatatcg ctttatagtt
      721 ttcactattg atgaactgct tgagctgctg tgataagggt taacttgtaa acatcatctg
      781 cattacatcc acgagaaagg tcgttaactg gtttgttcaa accttgcaaa acaggtccta
      841 cagctgcaaa accaccaaga cgttcagcca tcttgtaacc aatatttcct gcctcgatac
      901 ctgggaagat gaagacattt gcttgaccag ctaccgtact tccaggagct ttcagagctg
      961 cagtttcggg aacaaaggcc gcatcaaatt gcaactcacc atcgatttca aggtcaggac
     1021 gcaagtcgtg agcaatttta gttgcttcaa cgaccttatc aacgctttca ccaaaccctg
     1081 aacctttagt agaatagctc aacatggcaa ttttaggttc gatgccaaac atcttagctg
     1141 tgattgctga gttgatggca atttcagcca aggcttctgc atctggattg atgttaatgg
     1201 cacagtctcc aaataggtaa cgttccgtac cacgaaccat gaggaaggct cctgaagtac
     1261 gagttacatt tggacgagtt ttgatgattt gtagagctgg gcgaactgtt gaagctgttg
     1321 agtgaatcgc tcctgacacc attccatcaa ccaagcccaa gtaaaccaac atcacaccaa
     1381 agtagttgac atcttcaacc aaaaccttgc gtacatcttc ttcagtcatt ttgcccttgc
     1441 gacgctccac caaggcagaa accatttctt caaattgagg ataatgttga gggtcgatga
     1501 cctcataacc atccatgatt ccttcaattt caagataaat tttaattttt tcaggatttc
     1561 caagcaaaac aggaatcact tctgtttctt ttactaagcg ttttgttgct tgaagaatac
     1621 gaggctcttc cccttcaggg agaacgatac gagcattttt accaacaagg ttggctttga
     1681 gactttcaaa aacttccatg agttttctcc tttaagataa taattatact ctgagacagt
     1741 ttttatagag tattagttga taactgggta ataaggttac tgaaatcatc cggcaaggga
     1801 ctttctaact gcaagtcttg ctctaaaaat ggatgataaa aggataggta atggcaatgc
     1861 agagcctgac gttgaatacc atcttccaga ctaccaccat acaaatcatc tcccagcaaa
     1921 ggaaaaccga tatgagaaaa atggactcgg atttgatggg ttcgaccagt gtgcaggtga
     1981 atatagacca agtgaatatt tccataagaa gctacaatct tgtatgaagt atgggcatac
     2041 tttccgcctt tagccactcg tctggtaata atggaatctt catcacgcgc aatcggagca
     2101 ataatttccc cttctggctc caaatgtcca tctcccttaa ccaaagcaaa gtagcgtttc
     2161 tcgatagatt tcttctgcaa ctgcttgtct aatcgtgcat gggcataacc gtgcttggca
     2221 aagagcatca agccagaagt gtccctatct agtctggtaa caatgtgaac ctgctgattt
     2281 tcataatttt gcttgacata gtaacccttg ataaaattgg caatggtatt agagtgattg
     2341 acactaggaa tagaagccac tccatagggt ttattcaaga ctagaaagtg gtcatcctca
     2401 tagagaatat ctaatggatg ctcaatagcc tccaaggttt caaagccttc ctcagcggga
     2461 atgtaaatgg taacgtagtc tccaacgtcc aatagatacg ttgcattttg cggttgatta
     2521 ttgaccagaa tagctccacc tcgaaactta atcttggcca gcaatccctt agaaacctcg
     2581 tgctttttta aaaaggtctt aaccttgaca tgttcatctg cgataaattc aaacctcatt
     2641 catccacctc accgataaag gcatccttaa cacggttcca gaaactggta tggctaggag
     2701 tcgcgacaaa gtgaatctta tgatggtcga tttgatactc aatacgctca atattacgga
     2761 aagaataaac gctattgtca accgaaatag tatgataatc atttcttgtt ggaataagtt
     2821 caatcttatc cttcttaggc acaataatgg aagagcccag tgttcgataa acacgattat
     2881 taaggctggc aatttccgtt aattgcaaag cttcaatggt agggtgtaaa acagcaccgc
     2941 caagagactt gttataggca gtactaccag tcggtgtcga aactgttagc ccgtctccac
     3001 gaaaacgttc aaagggaaca caatttatta caatatctgc caccatggtt cgatcagacc
     3061 tgcggatgct ggcttcgttg agtgctctga aaatcttaac ttcaccattt tcaagaaaga
     3121 ccttcacatt cagaacaggg taagaaaccc ttgccccagt atctagctgc aaattagtca
     3181 ctagcttgtc caactcaaaa tcacgataat ctgtatagaa gcccaaatgt ccagtatgaa
     3241 gaccgataaa gcggaccttg tcaagctgat tttcgtactt atgaaaggcc gacaagagca
     3301 taccatcccc gccaatggaa atgacaatat ccggattggt atcattgagt ataaactgat
     3361 ttctcttcaa acgatctcgc aattcataca aaaccctttg actctgcggt tttctattgg
     3421 ctatcagatc aattcgttta cctgtattct tcatctgtat cgtcactgtt tcctacaccg
     3481 tcatttaatt ttctactcaa aggatcaaaa agtgcctggg cttcttggat atcatcacga
     3541 atttcaccca tttcttcatc caactgatgg gcgattctag ctgtaatttc cagtcgcttc
     3601 ttaatctcat ctgggaaatc cccttggtac ttgtagttga gagaatgttc tatcgttgcc
     3661 cagaaattca tggccaaagt acgaatttga atttctgcca aaatagtctt aactccattg
     3721 atggtatcaa ccgtatattc tactaccaca tgataggaac gatagcctga tgcttttcta
     3781 tgagtaatgt aatctcgctc ctgtatgatt cgcatatcct gacgcttgtg caaaatatcc
     3841 actacttcct tgacgtcatc tacaaactga accatcacac gtaagccagc aatatcctgc
     3901 aaatcgtgtt ccaaggtcgc ataagtaatg ccacgacgag ccattttttc tttgatgctc
     3961 tcaattggct taactcgacc ggtcacaaac tcaattggag aatgcttatt ttgcttacga
     4021 tattgcttac gaataccacg aagtttaatc tttaactcac caacagcttg aatgtaagga
     4081 tctagaaatt cttcccattc taaggtcata tattctccct tgttctcata ttatccgtca
     4141 aaaatatctt tgattttttc tccagattca tatacaataa atacaatgct tattatacca
     4201 taaatccgct ttcaacgata aaaaaaggta agaaaaatga aacatttaga aattgaattg
     4261 aaaacactat tgaaaaaaga tgaatacaat cgtctaaaag accagttcac aggtgtcact
     4321 cctgttcttc a
//
LOCUS       DAVKDZ010000123         4284 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000123 DAVKDZ010000000
VERSION     DAVKDZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.125"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            651..1643
                     /locus_tag="VWZ30_002070"
     CDS             651..1643
                     /locus_tag="VWZ30_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9335719.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYDGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1645..2463
                     /locus_tag="VWZ30_002071"
     CDS             1645..2463
                     /locus_tag="VWZ30_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU9335720.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVIYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2465..3250
                     /locus_tag="VWZ30_002072"
     CDS             2465..3250
                     /locus_tag="VWZ30_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9335721.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3289..3567
                     /locus_tag="VWZ30_002073"
     CDS             3289..3567
                     /locus_tag="VWZ30_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU9335722.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTRFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3552..3749
                     /locus_tag="VWZ30_002074"
     CDS             3552..3749
                     /locus_tag="VWZ30_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9335723.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
BASE COUNT         1187 a          735 c          918 g         1444 t
ORIGIN      
        1 gaatatgaaa gctttttata tcactattga aaaatttata cgagatgatg aaagccttaa
       61 gtgttatttt ataaaggtta tttcaagtcg ttccaaggta acaagtctag atcagattga
      121 agctgataaa acgatacaaa gaaaatattc aagtgagcta aaaaaattta ttggatttta
      181 taatgagatt atttgtgagg aaaatagttt cctacatgta cgaaagaggt ggtcgagttg
      241 gtttaggtag tcgatgcgtg agttgataat tctcagggta tggacttctt tttcatgaat
      301 gaggtaaaag agcaggtatt gtttagagac aatcattctg agcatatttt ctggatagag
      361 ggagtatccg attttatgat caaagttaat accgccctct ggtgagaaga tgagtaggtt
      421 ggtaatttaa actattaaac agaatttttg attaaaagta ttatttcatg agagaaatcc
      481 taatttcaca atccataggc aaacgcttgc atttcgtttt ttattggact ataataggtt
      541 ggtataaagc cttctgtagt aataaaatgt agaaggtgta gaaagtaagg atttagaata
      601 tttgtagtta aaaacacaat gttgctattc cttacgatag ggagatagat atggcaatga
      661 tagaagtgga acatcttcag aaaaattttg tgaagactgt taaggaaccg ggcttgaagg
      721 gggctttgcg ctcctttatt catcctgaaa agcagacctt tgaagcggtc aaggatttga
      781 cctttgaggt tccaaaaggg cagattttag gatttatcgg ggcaaatggt gctgggaagt
      841 cgacaaccat taaaatgctg acaggaattt tgaaaccaac atctggtttt tgtcggatta
      901 acggcaagat tccccaggac aatcggcaag attatgtcaa agatattggc gtagtctttg
      961 gacaacgcac ccagctatgg tgggatttgg ctctgcaaga gacctacact gtcttaaaag
     1021 agatttatga tgtgccagac tcgctctttc ataagcgtat ggactttttg aatgaagtct
     1081 tggatttgaa ggactttatc aaggatcccg tgcggactct ttcactggga caacggatgc
     1141 gggcggatat tgcggcctcc ttgctccaca atcccaaggt tcttttttta gatgagccga
     1201 ccattggttt ggacgtttcg gttaaggata atattcgtcg ggcaattact cagatcaatc
     1261 aagaggaaga aactaccatt cttttgacca ctcacgattt gagtgatatt gagcaacttt
     1321 gtgatcggat tttcatgatt gacaaggggc aagagatttt tgatggaacg gtgagccaac
     1381 tcaaggagac ctttggtaag atgaagactc tctcttttga actgctacca ggtcaaagtc
     1441 atctcgtctc tcactatgac ggtctgtctg atatgaccat tgatagacaa ggaaacagcc
     1501 tcaacattga atttgatagt tctcgctacc agtcagctga cattatcaag caaaccctgt
     1561 ctgattttga aatccgcgat ttgaagatgg tggatacgga tattgaggat attatccgtc
     1621 gcttctaccg aaaggagctc taggatgatc aaattgtgga gacgttataa accctttatc
     1681 aatgcagggg ttcaggagtt gattacttac cgagtcaact ttattctcta tcggattggc
     1741 gatgtcatgg gggcttttgt ggccttttat ctctggaagg ctgtctttga ttcttcgcaa
     1801 gagtctttga ttcagggctt cagtatggcg gatatcaccc tctacatcat catgagtttt
     1861 gtgaccaatc ttctgactag atccgattcg tcctttatga ttggggagga ggtcaaggat
     1921 ggctccatta tcatgcgttt gttgcgacca gtgcattttg cggcctccta tcttttcacc
     1981 gagcttggtt ccaagtggtt gatttttatc agcgttggcc ttccattttt aagtgtcatt
     2041 gtcttgatga aaatcatatc gggtcaaggt attgtagagg tgctaggatt aactgtcatt
     2101 tatcttttta gcttaacgct cgcctatctg attaactttt tctttaatat ttgctttgga
     2161 ttttcagcct ttgtgtttaa aaatctttgg ggttccaacc tacttaagac ttccatagtg
     2221 gcttttatgt cggggagttt gattcccttg gcattctttc caaaggttgt ttcagatatt
     2281 ctctcctttt tgcctttttc atccttgatt tatactccag ttatgatcat tgttggaaaa
     2341 tacgatgcca gtcagattct tcaggcactc cttttgcagt tcttctggct cttagtgatg
     2401 gtgggattgt ctcagttgat ttggaaacgg gtccagtcct ttatcaccat tcaaggaggt
     2461 tagtatgaaa aaatatcaac gaatgcatct gatttttatc agacaataca tcaaacaaat
     2521 catggaatat aaggtggatt ttgtggttgg tgtcttggga gtctttctgg ctcaaggctt
     2581 gaatctcttg tttctcaatg tcatctttca acatattcca ttcctagaag gctggacctt
     2641 tcaagagata gctttcattt atggattttc cttgattccc aagggaatgg accatctctt
     2701 ttttgacaat ctctgggcac tagggcaacg cctagtccga aaaggggagt ttgacaagta
     2761 tctgactcgt cccatcaatc ctctctttca tatcctagtt gaaacctttc agattgatgc
     2821 cttgggtgaa ctcttagtcg gtggtatttt attgggaaca acagtgacca gcattgtttg
     2881 gactcttcca aaattcctgc ttttcctagt ttgtattcct tttgcgacct tgatttatac
     2941 ttctcttaaa atcgcaacag ccagtatcgc cttttggact aagcagtcag gcgccatgat
     3001 ttacatcttc tatatgttca atgactttgc taagtatccg atttctattt acaattctct
     3061 tcttcgttgg ttgattagct ttatcgtgcc tttcgccttt acagcctact atccagctag
     3121 ctatttctta caggaaaagg atgtgttctt taacgtagga ggtttgatgt tgatttctct
     3181 ggttttcttt gttatttccc ttaaactttg ggataagggc ttagattcct acgaaagtgc
     3241 gggttcgtaa aagctaaagt aagactaaaa tcaagaaaga aacttatgat gtttgtaatt
     3301 gaagaagtca aggatgaaaa tcaaaaaaag gcagttgtag ctgaggtttt gaaggatttg
     3361 ccagaatggt ttggaatccc agaaagcaca caagcctata tagaaggaac cacgacactg
     3421 caagtttgga ccgcctatca ggagagtgat ttgactagat ttgtaagctt atcctattcg
     3481 agtgaagatt gtgcagagat tgattgtctc ggcgtaaaaa agcttatcaa ggtagaaaaa
     3541 ttgggagcca attgcttgct actttagaga gtgaagctcg taaaaaagtt ggttatctgc
     3601 aggtcaaaac agtggcagaa ggttctaata aagattatga tcgaacaaat gacttttatc
     3661 gaggtcttgg ctttaaaaag ttagagattt ttcctcaact atggaatccg caaaatcctt
     3721 gtcagatttt gattaaaaag cttgaataat attacttgac atctattctc agagtgctat
     3781 actgtaagtg taatcgccga tttagctcag ttggtagagc aaggcactcg taaagcctag
     3841 gttataggta gataaacgac tgaggatttg aaaaaataga taggtagaag ataaccgtta
     3901 agccttattc ttagcggtta tttatattgt ttaatagcgc tgatatttca tcaattatgc
     3961 ctgttttcgt gtttctggta gttgttcaag tttattgcta ctatttttga tggtatgaat
     4021 gtgcttataa tgtatcccgg ttaacgaaag ttttggactt atactcttag aaaatctctt
     4081 caaaccacgt caacgtcgcc ttgccgtgcg tatggttact gacttcgtca gtcttatcta
     4141 caacctcaaa acagtgcttt gagtaaccta cggttagctt cctagtttgc tctttgattt
     4201 ttattgagta ctaatcttat tttttaacgt gtgttaaaaa ataaattaag acatattaat
     4261 ttttcttgac ttaaattttt taaa
//
LOCUS       DAVKDZ010000124         4266 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000124 DAVKDZ010000000
VERSION     DAVKDZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.126"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            15..1637
                     /locus_tag="VWZ30_002075"
     CDS             15..1637
                     /locus_tag="VWZ30_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9335724.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            1699..4251
                     /locus_tag="VWZ30_002076"
     CDS             1699..4251
                     /locus_tag="VWZ30_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU9335725.1"
                     /translation="MKSFLKTYRIYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
BASE COUNT         1347 a          786 c          709 g         1424 t
ORIGIN      
        1 ataggagaaa aatattgctt acagtatctg atgtttcact acgttttagt gatcgcaaac
       61 tttttgatga tgtcaatatc aaatttacag aaggaaatac ttacggatta atcggtgcta
      121 atggtgccgg aaaatcaacc tttttaaaaa ttttagctgg agatatcgaa cctactactg
      181 gtcacatctc tcttggtcca gatgaacgtc tctctgttct tcgtcaaaat cactttgact
      241 acgaagatga acgtgccatt gatgtcgtta tcatgggaaa tgaaaaactt tatagcatca
      301 tgaaagagaa agatgctatc tacatgaagg aagatttctc agacgaggac ggggttcgtg
      361 ctgccgaact cgaaggagaa tttgccgaac ttggaggttg ggaagcagag agtgaagcct
      421 ctcaactact tcaaaaccta aacattccag aagaattaca ctaccaaaac atgagcgaat
      481 tggccaacgg tgaaaaagta aaggttctcc tcgccaaagc actttttggt aaaccagatg
      541 ttcttctctt ggacgagcct actaacggtt tggatatcca atcgattact tggttagaag
      601 acttcttgat tgactttgat aacacagtta tcgtagtatc ccacgaccgt cacttcttaa
      661 acaaagtttg tactcacatg gccgaccttg actttggaaa aatcaaactc tatgtcggaa
      721 actacgactt ctggaaggaa tcttctgagc ttgctgctaa attgctagca gaccgtaatg
      781 ctaaagcaga agaaaaaatt aaacaattgc aagaattcgt tgctcgtttc tctgccaatg
      841 cttctaagtc aaggcaggca acatcacgta agaaaatgct tgataagatt gagctagaag
      901 agattgtacc atctagtcgt aaatatccat ttatcaactt taaagcggaa cgtgagattg
      961 gtaatgatct cttgacagta gaaaatctaa ctgtaaagat tgatggtgaa actatcttgg
     1021 ataatattag tttcatcttg cgtccagatg ataagacggc acttattgga caaaatgata
     1081 ttcaaacgac tgcattaatt cgtgcaatca tgggagatat tgactatgaa ggaactgtca
     1141 agtggggagt tacaactagt caatcttacc taccaaaaga taactcagct gattttgcag
     1201 gaggagaatc aattcttgac tggttgcgtc aattcgcaag taaagaagaa gatgacaata
     1261 ctttcctacg tggtttcctc ggccgtatgc tcttctctgg agatgaagtt aacaaacctg
     1321 taaatgtctt gtcaggggga gaaaaagttc gtgtcatgct ttcaaaactc atgctcttaa
     1381 aatcaaatgt ccttgtactt gatgatccaa caaatcactt ggacttggaa tctatctcaa
     1441 gcttgaatga tggattgaaa aactttaaag aatcaatcat ctttgccagc catgaccacg
     1501 agtttattca aactttggct aaccatatca ttgtcttatc taaaaatggc gtcattgacc
     1561 gtatcgatga aacctacgat gaattcctag aaaatgcaga agtacaagca aaagttaaag
     1621 aactttggaa agactaaata aaacttcaaa actcagttgg gttaaccaac tgagttttct
     1681 atcattctac gaggtaacat gaaatcattt ttaaaaacat atcgaatcta ttttatttct
     1741 ttcatcattc ctgtagtgat tatgtctgga gtatatctat ctcaaggtat ctactggaat
     1801 agcgacaact ctcctctatt aggagatggc tttcatcaat acgttatttt tgatgtagcc
     1861 ttacgaaata tcctacatgg aaatagtagt ctgttttaca cctttacaag tggtctaggg
     1921 ctaaacttct atgccctatc tagttattac ttgggtagtt ttctcgcgcc tctagtttac
     1981 ttttttgatc taacgaatat gccagatgct gtctatctga ccactctctt aaaatttgga
     2041 ttgattggtc tgtcaacctt ttttagtttg aataaattgt ttcaatctat ccctcagact
     2101 ttaaaactag ccttatctac ttcctatgct ctgatgagtt tcactgtcag tcaattagag
     2161 ataaaaacct ggctagatgt ttttatcttg attcctttaa ttataactgg tttacatcta
     2221 ctgataactg aaaagaaact cctattgtac tttacaagtc tgtcaatctt atttattcaa
     2281 aattattatt ttggatatat gacagtattg tttcttattt tctggtatct ctgtcaaatt
     2341 tcgtgggact ttaagactcg aaaatcatct gttcttgatt tcatagttat ctccttttta
     2401 gctggtatgg ctagtttgat tatgactctt cccactctat ttgatttaca gacacatggg
     2461 gaaaaattga ctgaagttac aaagtttcaa actgaaagta gctggtatct tgatctcttt
     2521 gctaagcaat tcattggttc ctttgacaca acaaagtatg gggccatccc aatgattttt
     2581 gttggactac ttccctttat tttgaccatt ttatttttta cgctgaaatc tattaagttt
     2641 cacgtgaaac tcatatatgt aatattcttt gcatttctaa ttgcaagctt ttacatagaa
     2701 gctcttgact tattttggca aggcatgcat actccaaaca tgtttttaca tcgctatgct
     2761 tggattttct ctaccttgtt aatttacaca gcagcagaag tcttaaagcg tctgaaagaa
     2821 cttaaagtct ggaatttttt agtttcgctt tttcttgtag tagcaggatt tttagctacc
     2881 atctatctaa aatcgcatta ttctttttta acagatttga atattctgct tactcttgaa
     2941 tttttggttg tctattctct tttactcctt gcagttatca aaaagtttat atctgtgaat
     3001 ctatttgcca ttctaatctc tttatttata ctggttgaaa tgagtttaaa tgcttcatct
     3061 caaatggacg gaattgctaa ggaatgggga tttgcttctc gaagtgctta tagtcgagat
     3121 atcccagcta tggaatcttt ctcaacatat attggaaatc aatttactcg tactgaaaaa
     3181 ctacaaactc agacaggaaa tgacagtatg aaattcaact acaatggaat ctctcaattt
     3241 tcatctgttc gaaatcgttc atcaagctct actttagata aacttggttt taaatcctct
     3301 gggactaatc tcaatctccg atatgcaaat aatagtattt tggctgatag tttatttggt
     3361 atccagtaca atatctcaga cagtcctatc gataagtatg gctttaaaga tatctatcaa
     3421 aaagataatc ttaccctata tgaaaatcaa tactctcttc cgattgcagt tgctagtcaa
     3481 tctgtttaca atgatgtcaa gttcaatgaa cataccttgg ataatcaggc ctcattttta
     3541 aatcaacttg ctaacgtcaa ttttgattat ttttctccaa taccttatga aaaaacagaa
     3601 aaaatagaaa atactaatga tttgattagt gtcacaagtt cttcaaatga agatgcagca
     3661 atccagtatc aaattgaagt tccagaaaac agccaagttt atctctcttt cataaacctt
     3721 cacttttcta acgataaaca aaagaaggtt gacatccttg taaatggtga aaaaaagact
     3781 tttacaactg ataatgtctt ctccttcttt aatctaggat atactaaaga gaaaaaaact
     3841 ttcaatatca atgttagttt ccctggaaat tcacaagtat catttgaatc tcctaccttc
     3901 tatcgtttag ataccaaaac tttcaccgag gcaattcaaa aaattaaaga acaacctgtc
     3961 acagtatcaa cttctaaaaa caaggttttt gctacatatg atgtccaaca agatacatct
     4021 attttcttca ccattcctta tgacaaaggt tggtctgcct accaagatgg taagaaaata
     4081 gaaattaaac aagctcaaac tggatttatg aaagttgaca ttcccaaggg gaaaggaact
     4141 attacacttt ccttcattcc caatggtttt attactggag caatctgttc ctttacttct
     4201 ctcttactat ttggaatcta taatcacaga cgaaagtcat ctaaggcata aaaaatcgac
     4261 tagtta
//
LOCUS       DAVKDZ010000125         4202 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000125 DAVKDZ010000000
VERSION     DAVKDZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.127"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            92..460
                     /gene="mobC"
                     /locus_tag="VWZ30_002077"
     CDS             92..460
                     /gene="mobC"
                     /locus_tag="VWZ30_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEU9335726.1"
                     /translation="MENGEKRKRPVQKLIRLTEEENRFISTKVAESGMTNFNAFARIM
                     LIMGEVKILNFEELRELRKEINRIGVNINQVAKKVNEDDQASLTELSQILELQKYLKD
                     TVSQFIQKQENQTKEQERWL"
     gene            451..2127
                     /locus_tag="VWZ30_002078"
     CDS             451..2127
                     /locus_tag="VWZ30_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEU9335727.1"
                     /translation="MVVTKVLQIKGVASLGRSTKYIEDEAKTVELTDTKSLNNALRYI
                     ENPSKTSFLEQDEQFEFPAVVKDGRVVKQLVSTYGITDASTATEEFLLTKKNAAQRAG
                     TKERSDIHNPNRVLAHHIIQSFSPEDDWTPQEIHEIGRKTILELTGGQHEFVIATHMD
                     KGHIHNHIIFNSTNSVTLKKFRWQKNTARSLFNISNKYSDLAGAKILKERLWTNRKTY
                     HAYRKKNSFRYEIKSRLDFLLKHSTSLEDFKIKARALNLEVDTSGKEIKYLLTDRDQT
                     RNVRDRTLSKKGNYSLEKISEKVVANEVTLSVDEIKSEYEKMVAEREDDFEMRITVEE
                     WQVMEETKNGIYLEMEFGLRNKGTILIPAHQIERAEDGSYEIFIRKNDFYYFVNPEHA
                     DKNRYMKGETVASQLSYDNGEMIIRKNPKISTLDQLVREYNYLSAHGVTEGQQFDELL
                     NRFEEELEEVDNLLDKLDQRLGELNKIQSAFLALESSDPQEVKLAIAIMKDLRVDPST
                     RKSEIDKLVKEATFERQTLYQRVEAITKDYKLHQDIEKNVEQRKEIETSL"
     gene            2203..3009
                     /locus_tag="VWZ30_002079"
     CDS             2203..3009
                     /locus_tag="VWZ30_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEU9335728.1"
                     /translation="MKIITFAAIKGGVGKTTLTFNYGEWLARKGHNVLFIDLDHQCNL
                     TQCYNIYESQNTIANAFKGGDVDIKHIKENISLIPGSVQLDTVERDLENSDKKNMLLY
                     LWLEDNYEKKKLGQFDYILIDCRPDFATATKNAVAVSHAIISPLTPSEFGYNAKFNLS
                     TRLEAFRKDVIDYRTRESYITAELYFLANMIRPNYGSSRELLEALGLEAKEKGTDNLL
                     GIVPAKELFNHSTIDKVSIADMKEKPDLYQKHKKFFMELEETFSRIYDTI"
     gene            3062..3283
                     /locus_tag="VWZ30_002080"
     CDS             3062..3283
                     /locus_tag="VWZ30_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335729.1"
                     /translation="MAFTPKEKEISQIIEATHQPEQLPDIDSFESFERKKQFQFTLKP
                     SNRKKLDQVAKAAGAKSASDYLDKLIEKL"
     gene            3348..4124
                     /locus_tag="VWZ30_002081"
     CDS             3348..4124
                     /locus_tag="VWZ30_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEU9335730.1"
                     /translation="MDLITRLLAEREGKVHGGIYDITQKRFAYNSNRIEGSRLTEEQT
                     SLIYETKTIANIDAKGIKIDDLVEMNNHFKCFDYILDTVNEPLTEDHIKTLHRVLKSG
                     TSSEHNPIAPVGRYKVLQNEVGKIATASVEQTEDEMLSLLIGYDLKKQKDLNYLTSFH
                     AQFERIHPFADGNGRIGRLLLYKQCLKEGLTPFIVDDINKATYYSALEAFQVHDNRQL
                     LFDYFQSEQLFYMNYLKNKGFEGAINDEKEGDFIKKSDKF"
BASE COUNT         1561 a          619 c          838 g         1184 t
ORIGIN      
        1 acagcatttg gatatccaag tgctaatctt gccagaagaa aagggggcgg accccctcag
       61 tcaaaaaata gcagaataga ggtgtaaggt tttggaaaat ggagaaaaac gcaaaagacc
      121 agttcaaaaa ttaatcagat taactgagga agaaaatcgg ttcatttcaa caaaggttgc
      181 agaatctgga atgacaaatt ttaatgcttt tgcccgaatt atgttgatta tgggcgaggt
      241 caaaatttta aattttgaag aattgagaga attgcgtaag gaaataaatc ggataggcgt
      301 caatattaat caggtcgcaa aaaaggtaaa cgaagatgac caggctagcc taactgaact
      361 gagtcagatt cttgaattgc agaaatattt gaaagataca gttagccaat ttatccaaaa
      421 acaggaaaat caaacaaagg aacaagagcg atggttgtaa caaaggttct tcaaataaag
      481 ggtgtagctt ctttaggacg atctacaaaa tatattgagg atgaagctaa gacggtcgag
      541 ctgactgata cgaaaagttt aaataatgct ttacgctata tcgagaatcc atctaaaacg
      601 agttttttag aacaagatga acagtttgaa tttccagcag tggttaaaga tggccgagta
      661 gtcaagcaac tggtatctac ttatggaatt acagacgcta gcacagcgac agaagaattt
      721 cttttgacca aaaagaatgc tgctcagcga gcagggacaa aagaacgatc tgacattcac
      781 aatccgaatc gggttttggc tcaccacatc atccaatcat tttctcctga agatgattgg
      841 actccacaag aaattcatga gattggtcga aaaacaattt tagaattaac tggtggacaa
      901 catgaatttg tcatagcgac tcacatggat aaggggcaca ttcacaatca catcattttc
      961 aattccacta attctgttac attaaaaaag tttcgttggc agaaaaatac tgctagaagc
     1021 ttatttaata tttcaaacaa gtattctgat ttggctggag ctaaaattct caaggagaga
     1081 ttatggacta atcgaaaaac ctatcatgcc tatcgcaaaa aaaattcttt tcgctatgaa
     1141 attaaatcac gcttagattt tcttttgaaa cattcaacct ctttagagga ttttaagatt
     1201 aaggcacgag ctttaaatct tgaagttgat acttcaggga aggaaataaa atatctcctg
     1261 actgatcggg atcaaacaag aaatgtgagg gatcgtacac tgtccaaaaa aggaaattat
     1321 tctttagaga aaatttctga gaaagtggtt gctaacgaag taaccttatc tgttgatgaa
     1381 ataaaatctg aatatgaaaa gatggtggca gaacgagaag atgattttga aatgagaatc
     1441 acagttgaag aatggcaggt gatggaagaa acgaaaaatg gaatctatct tgaaatggaa
     1501 tttgggctta gaaataaggg aaccattctt attccagctc accagattga aagagcagaa
     1561 gatggctctt atgaaatttt tatcagaaaa aatgacttct actattttgt taatcctgaa
     1621 catgctgata agaatcgtta tatgaaggga gaaacagtcg cttcccagtt atcgtatgat
     1681 aatggagaaa tgattatccg aaaaaatcct aaaatctcaa ctttggatca gcttgttcgt
     1741 gaatataact atctatccgc tcatggagtg acagaaggac agcaatttga tgaattgcta
     1801 aatcgttttg aagaggaact agaagaagta gataacttac ttgataaact agatcaacgc
     1861 ttgggtgagc ttaataaaat tcagtctgca tttcttgcat tggaatcaag tgatcctcag
     1921 gaagtgaaac tagctattgc tattatgaaa gacttgagag tagatcctag cactcgcaaa
     1981 tctgagattg ataaacttgt taaagaggca acctttgagc gccagacttt gtatcaacgg
     2041 gtagaagcaa ttacaaaaga ttataagcta catcaagaca ttgagaaaaa tgtggagcag
     2101 cgtaaggaga tagaaacttc attgtaaaaa ataagtataa atatatttac aaatataatt
     2161 ataatggtat aatagaagta tagaaaagga tggagaaata gaatgaagat tatcacattt
     2221 gctgctatta aaggcggtgt tggaaaaaca actttgacat ttaattatgg agaatggttg
     2281 gctaggaaag ggcataacgt tctctttata gatttagacc atcaatgtaa tttgactcaa
     2341 tgctacaata tttacgaaag tcagaataca attgcgaatg ctttcaaagg aggcgatgtg
     2401 gatattaagc acatcaagga aaatatcagt ctaataccag gttcggttca gttggataca
     2461 gtggagcgag atttagagaa cagcgataag aaaaatatgc tcctttatct ttggttagaa
     2521 gataactacg aaaagaagaa gttgggtcag tttgactata tcttgattga ttgtagacca
     2581 gactttgcga cagctacaaa aaatgctgtt gcggttagtc acgctattat tagtcccttg
     2641 actccttcag agtttggtta taatgctaaa ttcaatctat caactcggtt agaagcgttc
     2701 agaaaggatg taatcgatta tcgtaccaga gagtcctaca ttactgctga gttatatttt
     2761 ctagcaaata tgattcgacc aaactatgga tcatcaagag aattattgga agcccttgga
     2821 cttgaagcca aagaaaaggg aacagacaac cttctaggga ttgtaccagc aaaagagtta
     2881 ttcaatcatt ctacgattga taaggtctct attgcagata tgaaggagaa gcctgatctc
     2941 tatcaaaaac ataaaaagtt ttttatggag ctagaggaga ctttttcaag aatttatgat
     3001 acaatataat tatatttgta aacacaaata caattataaa tataattaga aggagattat
     3061 gatggcattt acaccgaaag aaaaagaaat ttcacagatt attgaagcaa ctcatcaacc
     3121 tgaacagctt ccagatatag atagttttga aagttttgaa cgtaagaagc aattccagtt
     3181 tactttgaag ccaagtaatc ggaaaaagtt agatcaagtt gcaaaagcag ctggtgcaaa
     3241 gtctgcatca gattatcttg ataaattaat cgagaaacta tagttatgga tatatttata
     3301 actataaaca tatccataac tacaactgct tactacaagg agaaataatg gatttaatta
     3361 caaggctctt agcagagaga gaaggaaaag tacacggtgg tatttatgat attacacaga
     3421 aacgctttgc ttataattct aaccgaattg aaggaagtcg tctgacagaa gagcagacta
     3481 gcttaattta tgagacaaag acaatcgcaa atattgatgc aaaaggaatc aaaattgatg
     3541 atttggtaga aatgaataat cattttaaat gttttgatta tatcttagat accgttaacg
     3601 agccactgac agaggaccat attaaaacac tgcatcgtgt tctaaaaagt ggaacaagtt
     3661 ctgagcataa cccgatagct ccagttggtc gttataaagt attgcaaaat gaggtaggaa
     3721 aaattgcgac agcttctgtt gaacagactg aagatgagat gctttcttta ctgattggct
     3781 atgatttgaa aaaacaaaaa gatttgaatt acttaacaag ctttcatgca caatttgaaa
     3841 ggattcatcc tttcgcagat ggtaatggtc gaataggacg attgcttttg tacaaacaat
     3901 gcttaaaaga agggcttacc ccctttattg tggatgatat taataaggcg acttattatt
     3961 cagcgcttga agcctttcag gttcatgata acagacagct attatttgat tatttccaat
     4021 ctgagcaact tttttatatg aattatctta aaaataaagg atttgaagga gctatcaatg
     4081 atgagaaaga gggagacttc ataaaaaagt cagataaatt ctaagaaaag gtgcaacagt
     4141 ttaagctaag aggagaaagt catcacttaa aaaagttatt ataattatat ttacaaatat
     4201 aa
//
LOCUS       DAVKDZ010000126         4102 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000126 DAVKDZ010000000
VERSION     DAVKDZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.128"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..404
                     /locus_tag="VWZ30_002082"
     CDS             <1..404
                     /locus_tag="VWZ30_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9335731.1"
                     /translation="NTDYSVQELKNLYASRWGIETSFRDLKYSIGLVNFHAKKKEGIL
                     QEIFARFTNFNFCRWVTLQVAIDSSHKKQRYKVCFSDAAYACRLFFNGSLSSLQLKNY
                     LKKQLSIIRPNRKYSRKIKAQSVVDFIYRVT"
     gene            complement(544..1293)
                     /locus_tag="VWZ30_002083"
     CDS             complement(544..1293)
                     /locus_tag="VWZ30_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9335732.1"
                     /translation="MYHIKEAAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEF
                     KENIDKFGSGTAQYTSDAIAAYVQTNAEKIG"
     gene            1398..1619
                     /locus_tag="VWZ30_002084"
     CDS             1398..1619
                     /locus_tag="VWZ30_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEU9335733.1"
                     /translation="MIFLTDLRKHDRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGWPSGLVPFGSFPT"
     gene            1616..2867
                     /locus_tag="VWZ30_002085"
                     /pseudo
     CDS             1616..2867
                     /locus_tag="VWZ30_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851261.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(2889..3833)
                     /gene="manA"
                     /locus_tag="VWZ30_002086"
     CDS             complement(2889..3833)
                     /gene="manA"
                     /locus_tag="VWZ30_002086"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU9335734.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDTLLTKVPVKAG
                     DFFYVPSGTMHAIGPGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADNLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLFSILAGQGQL
                     TVDGKNYPIQKGSHFIVLSDVESWTLEGQDLELIVSHP"
     gene            complement(3954..>4102)
                     /locus_tag="VWZ30_002087"
     CDS             complement(3954..>4102)
                     /locus_tag="VWZ30_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU9335735.1"
                     /translation="AGTGEMNKRYGFIYVDRDNAGHGSFKRSKKKSFYWYKDVIDSNG
                     VSIE"
BASE COUNT         1085 a          897 c          795 g         1325 t
ORIGIN      
        1 cgaatacaga ctattcagtc caagaattaa aaaatctcta cgccagtaga tggggcatag
       61 agactagttt tcgtgacctg aaatacagta ttggcttggt caattttcac gccaaaaaga
      121 aggaagggat tctccaagaa atctttgctc gctttacaaa ttttaacttt tgtcgttggg
      181 taaccttgca agttgccatc gacagtagtc acaaaaaaca aagatataaa gtgtgtttct
      241 cagatgcggc ttatgcctgc cgtttgtttt ttaacggttc cctttcttcc ctccagttga
      301 aaaactacct caagaaacag ttatctatta ttcgaccgaa tcgaaaatat tcaagaaaga
      361 taaaagctca atcggtagtt gatttcatct atagagtaac ataaatctca aaaaataggg
      421 tgaaaaaacc tttatttgtc atgcaaaaaa atagaagatg tgatagagaa tcactttgat
      481 gatcccaatc acacctccta ttcaagtagt tagatcacta acttaatgac attgaggaaa
      541 ttcctagcct attttttctg catttgtctg aacgtaagct gcaatggcat ctgacgtgta
      601 ctgggctgtt ccagaaccaa acttgtcaat gttttcctta aactctgggt tgtagacgta
      661 acctttaccg atatgaccga atacctcaat agagcagtca aatccataag tgcgaatggc
      721 ttgcaagagc ttggctgctt gctcttggtt ttcggttgct gttgcaggta aaccaacttg
      781 aagattttgt gccaaagttt gaaagacttg gttaaaggcg gccgtagcct cgtcttcgtg
      841 acctttttgg cgttcgagcg cttgtcccat gacttcttga ccatatttct ctaccgcttc
      901 ttggtggtat ttttgattgt cttgatagct aaatcccgtg aatttttcct caatggtcat
      961 ttttctttct cctttttgtt cttgaatagt tttttgcaag gtggaaatca aggtatccag
     1021 atgttgcctt tcgcgagtta gatagtccaa ctgcctagtc aaatggggca ataaatctgt
     1081 cctttcttcc tttaacagct ctgctatttt ctctaaagaa aagcctagat atttgtagta
     1141 aagaatgacc tgaaggcgtt ccaaatcctc ttgactgtag gttcgatagc cgttttccga
     1201 ctttaagggg accaagagtc ctatcttgtc atagtgatgc agggtcttga cagagacacc
     1261 tgaaagctgc gcagcttctt ttatatggta catgatttcc tccttacaat gatagtataa
     1321 cccctcccct taggtcagag tcaagcgttt ttgagaaaat tttttaactt ttttgagaaa
     1381 ggtgtgaaaa cttgaaaatg attttcttga cagaccttag aaaacatgat aggatagtca
     1441 agtctatcaa tcaaacagaa ggctatttga caactcaagt agctttttct tattttgaaa
     1501 aaggagatca gagtttaact atgtcagaaa aatcacaatg ggggtcgaaa cttggtttta
     1561 ttctagcatc tgctggctgg ccatcgggct tggttccgtt tggaagtttc cctacatgac
     1621 tgctgctaat ggcggtggag gctttttact aatctttctc atttccacta ttttaatcgg
     1681 tttccctctc ctgctggctg agtttgccct tggccgtagt gctggcgttt ccgctatcaa
     1741 aacctttgga aaactgggca agaataacaa gtacaacttt atcggttgga ttggcgcctt
     1801 tgccctcttt atcctcttat ctttttacag tgttatcgga ggatggattc tagtctatct
     1861 aggtattgag tttgggaaat tgttccaact tggtggaacg ggtgattatg ctcagttatt
     1921 tacttcaatc atttcaaatc cagccattgc tctaggagct caagcgacct ttatcctatt
     1981 gaatatcttc attgtatcac gtggggttca aaaagggatt gaaagagctt cgaaagtcat
     2041 gattcctctg ctctttatca tctttgtcgt catcatcgaa cgctctccca gtttgccaaa
     2101 tgccatggaa ggggttcttt acttcctcaa accagacttt tcaaaactga ctagcgctgg
     2161 tcccctctat gctctgggac aatctttctt tgccctctca ctaggggtta ctgtcatgtt
     2221 gacctatgct tcttacttgg acaagaaaac caatctagtc cagtcaggaa tctccatcgt
     2281 agccatgaat atctcgatat ccatcatggc aggtctagcc attttccaag ctcggtcccc
     2341 cttcaatatc cagtctgaag ggggaccgag cttgctcttt atcgtcttgc ctcaactctt
     2401 tgacaagatg ccttttggaa ccattttcta cgtcctcttc ctctttgcga ctgtcacttc
     2461 ttctgtcgtg atgctggaga tcaatgtggg caatatcacc aaccaggata acagcaaacg
     2521 tgccaaatgg agtgttattt taggaatttt gacctttgtc tttggcattc cttcagccct
     2581 atcttacggt gtcatggcgg atgttcacat ttttggtaag gccttctttg acgctatgga
     2641 cttcttggtt tccaatctcc tcatgccatt tggagctctc tacctttcac tttttacagg
     2701 ctatatcttt aaaaaggctc ttgcaataga gaaactccat ctcgatgaaa gagcatggaa
     2761 acaaggactg ttccaagtct cgctcttcct tcttcgtttc gtcattccaa tcatcatcat
     2821 tgtggtcttc attgcccaat ttatgtaatc aaaaaggact tgagtagtga actcaggccc
     2881 tttctttttt atggatgact aacaattaat tctaaatctt gcccttctaa agtccaagat
     2941 tcaacatcac ttagtacgat aaagtggctc cctttttgga ttggatagtt ttttccatca
     3001 acagtcagtt gaccttggcc agctaagata ctaaacaaac tgtaatcagc tgtcttctca
     3061 aaatcaactt ttccagtgat ttcccacttg taaactgcga agaaatcatt agaaacaagt
     3121 aaagtggaac gcaaattatc tgctttaaca gttacaggac ggctatttgc tggctcgcca
     3181 atgttcaaaa catcgatgga tttttcaagg tgaagttcac gcaagttgcc cttatcgtcc
     3241 tttcgatcaa agtcatagac gcggtaagtt gtatcactag actgttgggt ttcaagaatc
     3301 aagatgccag gaccgatagc atgcatggtg ccacttggta catagaagaa atctccagcc
     3361 ttgacaggga ctttggtaag caaggtatcc cagttcttgt cctcgatttg ctggcggagt
     3421 tcttcttttg acttggcatt gtggccgtag ataatctctg aaccttcatc cgcagcgatg
     3481 atgtaccagc attctgtttt tccaagttcg ccttcatgct ctagtccata agcatcgtct
     3541 gggtgaactt gaacgctgag ccagtcgtta gcatccagaa tcttagtcaa aagcggaaat
     3601 acaggctcag gacgattgcc aaacaattcg cggtgttccg catacaaagt agcaagatct
     3661 gttccctcgt aacgaccatt ggcaacttta gaaacaccat ttagatgagc tgagatagcc
     3721 cagtactctc cgactttttc acttgggatg tcgtagccaa actcatcacg tagcttggta
     3781 ccaccccaga ttttttcttg cataactgat tgtaaaaata atggttctga catcttaatc
     3841 tcctgtctga tttttctccc ctcattatag caaaaaaaga gttccatttg aactcttttt
     3901 tacatcttat aaagcaggga gaagatttta taaaaatagt aaacaaatgt gctctactcg
     3961 atgcttacac cattgctatc aatgacatcc ttgtaccaat agaaggactt cttcttgctc
     4021 cgtttgaaac ttccatgacc agcattatct cgatccacat agataaagcc atagcgcttg
     4081 ttcatttcgc ctgttccagc tg
//
LOCUS       DAVKDZ010000127         3954 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000127 DAVKDZ010000000
VERSION     DAVKDZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.129"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(361..894)
                     /locus_tag="VWZ30_002088"
     CDS             complement(361..894)
                     /locus_tag="VWZ30_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU9335736.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(983..1759)
                     /gene="recX"
                     /locus_tag="VWZ30_002089"
     CDS             complement(983..1759)
                     /gene="recX"
                     /locus_tag="VWZ30_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU9335737.1"
                     /translation="MKITKLEKKKRLYLMELDEQQTSYITEDTIVRFMLSRDKVISKE
                     ELTEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTHKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPSRALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVNQETIQELILKELDK
                     QYSKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            complement(1769..2545)
                     /locus_tag="VWZ30_002090"
     CDS             complement(1769..2545)
                     /locus_tag="VWZ30_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3'-phosphotransferase"
                     /protein_id="HEU9335738.1"
                     /translation="MNSKTPRFSILDFPEQLRSYIKGANLSDSSSHSSATVLYIDSGY
                     YLKIDQKGRLAQEAKIASWFETEGLGTPVITYLSANKDFLLTKEAIGSNALAFLNQPE
                     RLCQTLAQALNKLHSLKPQSFPSENHLKRYKEKALKNYQKGTFYNKTLLPQFHIHSRE
                     EAYQLIQEKGYILKADAFIHGDACLPNFILKDASHFSCFIDLGLAGFSDRHIDLFWAI
                     WSLHYNLQDATYAELFLDYYGREYVNMDKLRLVAAFEAFR"
     gene            2602..3954
                     /gene="rlmD"
                     /locus_tag="VWZ30_002091"
                     /pseudo
     CDS             2602..3954
                     /gene="rlmD"
                     /locus_tag="VWZ30_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050284.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
BASE COUNT         1247 a          686 c          726 g         1295 t
ORIGIN      
        1 taaatagcat tttctctgtt gagatattgt ttttaaaata ctgtactaaa tgattgatgc
       61 tatgtggaaa tacaaaaaaa tgtttttgat acgaagttga cctgtatttt ttatactaat
      121 cattttcgta ttttttgtat taaacgatat aagtttgttg taaacttaca aggaataaag
      181 acattaaaaa ataacagtat atctatttgt tttatatatt ttacgaattc tgcataaatc
      241 tctttctagt aatgtgttgt aactctgcta taatggattt attccttttt gtgtttacac
      301 aatttatttt atactaccaa aaaaacaagg tcaggatttt gttcctgacc tttgacaact
      361 ttaccgattc tttagttcta catagcgctt gtaccaaatg tttacatagg cttctgagaa
      421 aggaccacgt ccattgttaa tccaatcaac aagaattttg acatgttctt ttaaaatata
      481 gtccaagtca tcagaataat tcattttgcg tttgtgacgc tcgtactctt caacgtccaa
      541 gagacgtttt tctccatctg taaaaatttt aacatccaaa tcgtaatcaa tatacttcag
      601 tgcttcttca tccagatagt aggggctagc catattgcaa tagtaagaag ttccattatc
      661 acgaatcatg gcaatgatat taaaccaata tttcttgtga aagtaaacaa tagccggttc
      721 tcgagtgacc caacgacgac catcactttc ggtaacaagt gtatgatcgt tgacaccgat
      781 aatggcgttt tctgttgttt ttagtaccat ggtgtcccgc caagttcggt gaagactccc
      841 atcatgctta taactttgaa ttgtaataaa gtcgccttct tttggaagct tcataactaa
      901 ccaactttct acaatttata agtttatcat ttactattgt accataaaat tacctaaaat
      961 ctgtgaattt cacttggaaa tattaaagat attctctaag agcgcttgct atatccgaaa
     1021 aatcgtagcc ctttcgtgct aaaacttgag ttaaacgctg cttcagttcg tatccttcat
     1081 actttcgagc atacttacta tattgtttat caagttcctt taagatgagt tcttgaatcg
     1141 tttcttggtt aacttggcta tctaagtcgt caaaggcaat tttagcatca gaataagaga
     1201 agcccttgtt agtcaagttc tggataatct tatcttgcag ggcacgagat ggaagttttc
     1261 cctcatattt tttcaatagt ttattagcta cacgttgagc aacttcagaa aaatcaaatt
     1321 ctttcaagtt ctcttctata gtagattttg aaatcccttt ttgagctagt ttatgagtca
     1381 gcacataagg acccttgtct cctgaaagtt gattggtatt gatgatagca taagcgtact
     1441 gaccatcatt aatccactta tcttctttaa gattagcaat aacttgagaa acgatgtttt
     1501 tatcaatatc gtattttttc agatattctc tgacttcttt ttcagtgcgt gctttaaagg
     1561 ataagtggta gagagcgaga ttcttaccat aagaaaactg ggcaaaatcc tgaatctcgg
     1621 tcagttcctc tttactaatg accttatctc gagacagcat aaaacggaca atggtatctt
     1681 ccgtgatata agaggtttgc tgttcatcta gttccatcaa gtagagacgt tttttctttt
     1741 caagttttgt gattttcata atttcctttc atctaaatgc ttcgaaagct gcaacaagtc
     1801 gtagtttatc catatttaca tattctctac cgtaatagtc gagaaataat tcagcatacg
     1861 tagcatcttg taggttgtaa tgaagggacc aaattgccca aaagaggtcg atatgtcggt
     1921 cactaaaacc agccaatccc aagtcaataa agcaagaaaa atgagaggca tccttcaaga
     1981 tgaaatttgg caggcaggca tctccatgaa taaaagcatc agctttgaga atgtaacctt
     2041 tctcttgtat cagttggtaa gcttcttcac gactgtgaat gtgaaattga ggcagaagag
     2101 tcttgttata aaaagtcccc ttctgatagt tttttaaggc tttttcttta taacgcttca
     2161 gatgattttc cgacggaaaa gattgtggtt taagactgtg aagtttgttt aatgcttgag
     2221 ccagggtttg acagagtctt tcgggttgat ttaaaaaagc gagagcgttg ctgccaatgg
     2281 cttctttagt cagaaggaag tccttatttg ctgataaata ggtaatcact ggagttccca
     2341 atccttcagt ttcgaaccaa cttgcaatct tagcttcttg agctaatctg cctttttgat
     2401 ctatttttaa atagtaacct gaatctatat ataggacagt tgcacttgaa tgagaagaac
     2461 tatcagagag attagctccc ttgatatagc ttcgtaattg ttcagggaaa tctagtatac
     2521 taaagcgagg tgtttttgaa ttcatgcttt tattgtaaca tattcttaaa tatcttagaa
     2581 actccatggt ataatagaca tatgaatctg aaagtgaaac aaaaaatacc attaaaaatc
     2641 aaacgcatgg gaattaatgg tgagggaatc ggcttttacc aaaaacatta gtctttgtac
     2701 ctggagctct caaaggcgaa gatatctatt gtcagattac ttctattaga cgcaactttg
     2761 ttgaagcaaa attactgaag gtcaacaaga agtctaaatt tcgaattgtg ccatcttgta
     2821 ctatttataa tgaatgcgga ggctgccaaa tcatgcacct gcattatgat aagcagctgg
     2881 agttcaagac ggacttactt catcaagcgc tgaaaaaatt tgctcctgca ggatatgaaa
     2941 attatgaaat tcgtccaact attggaatgc aggaaccaaa atattacaga gctaagttac
     3001 aatttcagac tcgaaaattt aaaaatcagg tcaaggcggg cttatatgca caaaactctc
     3061 actatttagt agagttgaaa gactgcctgg tacaagataa ggaaacccaa gtgattgcta
     3121 atcgcttagc agaattactt acttatcacc agattccaat cacggatgag agaaaagttc
     3181 taggtgtccg tactattatg gtccgacgcg cgagaaagac cggacaggtt cagattatta
     3241 ttgttacaaa ccgccagctt aatttaaccc aactggtaaa agagttggtt aaagattttc
     3301 cagaagttgt gacagtagct gttaatacaa atacagctaa aaccagtgag atatatggtg
     3361 aaaagacaga gattatctgg gggcaagaga gtattcaaga aggtgtactc aattatgaat
     3421 tttcactatc ccctcgagct ttttatcaac taaatcctga gcaaacagaa gtcctctata
     3481 gcgaagcagt aaaagcgctg gatgttgata aagaagacca tttgattgac gcttattgtg
     3541 gagttggaac gattggattt gcctttgcaa agaaagtaaa aacactcaga ggtatggata
     3601 ttattccaga agctattgaa gatgccaagc gaaatgctaa aagaatggga tttgacaata
     3661 ctcattatga agctggaacg gcagaagaga ttattcctcg ttggtacaag gaaggctacc
     3721 gagcagatgc tttgattgtt gacccaccac gtacaggtct ggatgataag ttattagata
     3781 ctattcttac ttatgtacca gaaaaaatgg tttatatttc ttgtaatgtt tcgaccttgg
     3841 ctcgtgattt ggtacgctta gtagaagtct atgatcttca ttatatccag tcggtcgata
     3901 tgttcccaca tacagctcga actgaagctg ttgtaaaatt aataaaaaaa gttt
//
LOCUS       DAVKDZ010000128         3822 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000128 DAVKDZ010000000
VERSION     DAVKDZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.130"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(108..311)
                     /locus_tag="VWZ30_002092"
     CDS             complement(108..311)
                     /locus_tag="VWZ30_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9335739.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(304..1386)
                     /locus_tag="VWZ30_002093"
     CDS             complement(304..1386)
                     /locus_tag="VWZ30_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9335740.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVPADQEPILFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLNKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1512..2294)
                     /locus_tag="VWZ30_002094"
     CDS             complement(1512..2294)
                     /locus_tag="VWZ30_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU9335741.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVIVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2294..3637)
                     /locus_tag="VWZ30_002095"
     CDS             complement(2294..3637)
                     /locus_tag="VWZ30_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU9335742.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGVDSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
BASE COUNT         1080 a          763 c          869 g         1110 t
ORIGIN      
        1 ggggtcagtt cattctacac ccactattat gcgttttatt gtgggaagat tttcctcatt
       61 ttctagtgaa attgaacttt ttctaacatc ttttgataga tgacttctta atcaattatc
      121 atttccattg ttgtttcttg gggtttcatc ccttgtcttt gataaaagcg gagagcaccc
      181 tcgttatcat tccagacact tagggttaga ttattgcagc caatttcttt agcataatag
      241 cagaaaaatg acaataaaat agcccctaga tattttgaaa ggaaagcata ggggctatat
      301 agtttaatca aaataaagca aatctttgct ggtgcttgta aagaggtcga agccatcctt
      361 ggtaacaaca ccgcagtctt caatacgaac accgacttta ccagggatat agatacctgg
      421 ttcaacagag aagcacatgc cttcttcgat gaccatgtcg tttccttcca tgatagatgg
      481 gaattcatgg acatccatac cgataccatg cccgagacgg tggttgaagt actcaccata
      541 accagctttt tcgatgacct cacgggcagc gcggtccact tcatgagcag tcacacctgg
      601 cttgataaag tcaagagcag cttgttgggc ttcaagagtc aagttgtaaa tatctttctt
      661 gaattggtct ggtttgccga cagcgactgt acgagtcata tctgacgcat agccattgac
      721 cagaacaccc aggtcaaaga ggagaagagc atcattttca accttattag ctgctggaat
      781 gccgtgtgga ttcgcagcat tatcaccagt caagaccatg gtatcaaagc tcatttcata
      841 accttcacgt ttcatggcaa agtcgatttg tgcgatgata tctgtctcag tcttattaag
      901 agaaatattg tcaaaaccaa catgaacagc cttgtcagca taaagacctg caaccatcat
      961 tttttgcact tcatcagctg atttgatgag gcgcatgcgt tggatacgag gagtgaggtt
     1021 gtcaaactca gcagtctcaa aaactgtttt caaaccatgg tatttggtca agatgagatt
     1081 gtcaaactca acagcgacac gtttgaagtc aagttgtgga agagcatgtt tgattttttg
     1141 ccatggattt tcagaatcga catagcccac tactgggaag gaaacggtgc tacttgcacg
     1201 ttctacttca agagctggga caaagaggat aggttcctga tccgctggga caaagaggaa
     1261 catttggcgt tcatggggat cactgtaaaa accagtgagg taattgattg tgacggggtc
     1321 agatacgaca gcgacgtcta gtttttctga ttcaagatat gttaggattt gttgtaattt
     1381 agacatatgc taccttcttt ctacccctct attttggcaa aaaatgagag aaaatgcaag
     1441 ggagaagggt aaaagaaaag acaaaaaact ccgacaagat aacggagttc tttgatatca
     1501 ttttcctttg tttaagaaaa gtcagccttg cttttgacgt cactgtactc ttcagcgatt
     1561 tcttggctga gaatatcctc ataggcaggg agttggatgt cctgaccata tttcttcttg
     1621 agggcagtag taactaggcg ataagccaga ttggcattac gagagaggat aggcccgtgg
     1681 aagtaggaac caaagacatt cttataatga accccttcac cgaccttttc ttcgttgttt
     1741 ccatttccat agacaacctg tcccagcggt ttttggtcat cagagaggaa ggtacgacct
     1801 tggtgatttt caaatccata gtaggtttca tcgaaatctt cattgtgaat cttgatgtca
     1861 ccgataaaac ggttattggt ctggttgagc gtgtagtgtc ccatgacccc tagcccttcg
     1921 atacgttttc ctgaagcttc aacataatat tgacccaata gttggaaacc accgcagata
     1981 gccagaacta caccgtcgtt ttggatgtag ttgtcaatgc tctctttttt agcaggtagg
     2041 tcgtctgcaa tgatactttg ttcaaagtct tgaccaccac cgaaaaaggc gatgtcgtag
     2101 tgattttcat caaagtcatc atggagagaa acgatgtcaa cgatcacatg ggctcccagt
     2161 ttttcagcca catacttgag catgaggatg tttccattgt ccccgtaggt attcatgaga
     2221 tttccgtaga ggtgggcaat gttgagctga taggggtaat tgccatcttt tgaggaaagt
     2281 gaagtataaa ccattagttc atctcctttc taacaatctg acgactagcc agcagttcac
     2341 gaaattccag catggcagta taagttgcca gaatataggc atgcttgcag tcttgattct
     2401 caatggtctt gagaacttgc tccagattac tcgtttcagt gattttctca gctggatagc
     2461 cagtcactcg gaggcgacga gcgatttcag aatgacgaac accgccagcg ttgatttcag
     2521 gaatgtccat gtcagtgatt tgttcaaagt ctgcatccca gatccagcta gtgtcaattc
     2581 catctgcata gttggcatta aggaggacag ataggctaaa tggataaggt gctagtttga
     2641 tcatttcgat agcttgggtt gcaccgactg gatttttaat caagacaagg gtacattcct
     2701 tgtcaccgat atgaaaggtt tcttggcgtc caaagacagc acggctcttg tcaaatccct
     2761 gtttgatgag ttgcgaatcg acacctagga aacgggcgat ggccacagca gctagggcgt
     2821 tatagatatt atagagcccg ccgatttgga taccgtattc ttggccgtct atgacaaagc
     2881 gagagcgatt gttggtcaac tcaaccagtt ttgtcaaacg atagtcgaga tcaggacgtt
     2941 tacatccaca accttcacag atataggcac ccaagtttgc ataggtatta tgctcatatt
     3001 tgaggatgcc ttggcagtca ggacagagaa tcccttcggt attgtagtga gccagttggg
     3061 ctggtccttt ttccaagtca aaaccaaaat actctatagg gtttggaata gttggcttgt
     3121 agaaaagtgg actgtctccg ttaaggagaa cagtagcagt tggaactttc cgaatggcat
     3181 ccaatatcat gttataggta gtatagattt caccgaaacg gtccatctgg tcacggaaga
     3241 tattagtaat gacaaaaaga ctaggctgga tatagtcaca gatacgagat agactggctt
     3301 cgtcgatttc taggacggca atatttttcc cagttttaga agatttggct gttaagaagg
     3361 ttgtcgcaat tcctgtaatc atgttggcac cgcttgggtt ggtcagaact tgaccataaa
     3421 cttcttttaa aataccgaca gtgagggcag ttgtcagggt ttttccattt gttccagtga
     3481 caacgacaat ctcgtagttc ttagctaggc tttgtaaaat atctttatca aattgaaggg
     3541 cgactttccc tgggagcgta cttccacgtc caagacggct taaaacgaag tgggaagaac
     3601 gcccagcaag aaggcccaaa gtagttttta agttcatgtt tctattatat cacaaaagag
     3661 ggaaaagaca gatttggtgt ttcgtagaag taaaaacagc ccaaagaggg ctgtcagtta
     3721 agatgagatt tcaacttaaa tcgcaaacaa gtcattcaag ttctcagcca aggttgggtg
     3781 agtgaagatt tgttttgtga agtaagtgta aggaatcttg tt
//
LOCUS       DAVKDZ010000129         3795 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000129 DAVKDZ010000000
VERSION     DAVKDZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.131"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(71..604)
                     /locus_tag="VWZ30_002096"
                     /pseudo
     CDS             complement(71..604)
                     /locus_tag="VWZ30_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1050..2396
                     /gene="gdhA"
                     /locus_tag="VWZ30_002097"
     CDS             1050..2396
                     /gene="gdhA"
                     /locus_tag="VWZ30_002097"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU9335743.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVISFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGSVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKIYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
     gene            complement(2853..3795)
                     /locus_tag="VWZ30_002098"
                     /pseudo
     CDS             complement(2853..3795)
                     /locus_tag="VWZ30_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520162.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         1222 a          706 c          650 g         1217 t
ORIGIN      
        1 aatatctata agggatttac ccactacaaa tattatagag ccagagattt ttatttacct
       61 agtatagctc ttatcttaga catttacaag agtgggacaa aagagcctcg taaaaggtat
      121 tgcaacttgg taataccctt ttgaggtgct ttttgatatg agcccatgtt ttctcaatag
      181 gattgtactc aggtgagtag ggaaggagag gtaaaagttt attcccaaac tcttcacaca
      241 agacttctag cttacccatt ctacggaatc ttgcattatc cataataata accgatggtg
      301 tatttaatgt tggtaagaga aacttctgaa accaagcttc aaaaaagtcg ctcgtcatcg
      361 tctctgcgta agtcattgga gcgattaact caccatttgt tagccctgca accaaagaaa
      421 tcctctgata tattcttcca gatactttac ctcttattaa ctgacctttt aaagaacaac
      481 catactttcg ataaaaataa gtatcgaatc ctgtctcatc aatataaaca ggtgctaagt
      541 gctttaaact attaaaactt ttaagaaata aggctacttt ttctaggtct tgttcatgga
      601 tagtatagta ctgtatggtt accgaacaag tctaatgaaa aaattctgat gatagttcta
      661 aatctcaata accgcttggg ttccatgatt caactccagc aagacatcta tacttgtata
      721 aaaaatcctg ccatgaataa ggagatgtaa atattgcttt cctccaaaat gatcatagtt
      781 ttactggcaa atctaacata tcacggataa attaacaagt gatttctgaa ttgctaaaca
      841 ttttcttttc ttatagcata ctttaagatt ttctctttga gaaagatatt tccaagaaaa
      901 aggtttgatt tttatcagtt caagaaaagc cgaaaccaaa aaacaaattg aaagaatttt
      961 tagaaaattt ttgtttttct ctttacagtt taaaaaaatt ctgctataat agctcatgta
     1021 ataaaatatg acaacaaaag gagaacgata tgacatctgc taaagaatat atccaaagcg
     1081 tgtttgaaac tgtaaaagct cgtaacgggc acgaggctga attcctccaa gctgttgaag
     1141 aatttttcaa cactttggaa cctgtatttg aaaaacaccc tgagtatatc gaagaaaata
     1201 tcttggcacg tattactgag cctgagcgcg taatttcttt ccgtgttcct tgggttgacc
     1261 gtgatggaaa aattcaagta aaccgtggtt accgtgttca attcaactca gctgttggac
     1321 catacaaagg aggacttcgt ttccacccaa ctgtaaacca agggatcttg aaattcctcg
     1381 gatttgaaca aatctttaaa aacgtcttga ctggacttcc aatcggtgga ggtaaaggtg
     1441 gatcagactt cgatcctaaa ggtaaaacag atgctgaagt gatgcgtttc tgtcaaagct
     1501 tcatgactga attgcaaaaa cacatcggac catcacttga cgtacctgct ggtgatatcg
     1561 gtgttggtgg acgtgagatt ggttaccttt acggtcaata caaacgcctt aaccaatttg
     1621 atgctggtgt cttgactggt aaacctcttg gatttggtgg tagcttgatt cgtccagaag
     1681 caactggtta cggtttggtt tactatactg aagaaatgct caaggcaaat ggcaacagct
     1741 ttgctggtaa gaaagtcgtt atttcaggtt ctgggaacgt tgctcaatac gctcttcaaa
     1801 aagcgactga acttggcgct actgtcatct ctgtatctga ctcaaacggt tatgtcatcg
     1861 atgaaaatgg tatcgacttc gatcttttgg ttgatgttaa agagaaacgt cgtgctcgtt
     1921 tgactgagta tgcagctgag aaagcaactg caacatatca cgaaggttct gtatggactt
     1981 acgctggaaa ctatgatatt gcgcttccat gtgctactca aaacgaaatc aacggtgaag
     2041 cagctaaacg tttggttgct caaggcgtta tctgtgtatc tgaaggtgcc aacatgccga
     2101 gcgaccttga tgccatcaaa atctacaaag aaaatggtat cttctacgga cctgccaaag
     2161 ctgccaacgc tggtggtgta gccgtttcag cccttgaaat gagccaaaat agccttcgtc
     2221 tctcatggac tcgtgaagaa gttgatggac gtctcaaaga catcatgaca aacatcttca
     2281 acacagctaa aacaacttca gaaacatatg gtcttgataa agactatctt gcaggagcaa
     2341 atattgctgc atttgaaaat gtagcaaacg ctatgattgc ccaaggtatt gtttaagatt
     2401 tatttgctta atcctcaatc actacgattg gggattttta gttgagttag acagttggga
     2461 aattctaatt aaatgaaata aaaactgaac aaattgattg cggaattcaa accaatttct
     2521 aacatatttc tagaaattaa tttgactttc ctaatcgatt tgttcatatc ttatttcaat
     2581 ttactatact tacttgaccc tttttatact caatgaaaat caaagagcaa actaggaagc
     2641 gagccgcagg ctgtacttga gtacggcaag gcaaagctga cgtggtttga atttgatttt
     2701 caaagagtat taaacaaaaa gaaaaaccgc tactcctaaa aatagcgatt taatcatgtt
     2761 tttaagctac tactgtagtt gctctattat ttaagagtaa caatttgcaa atcctatttt
     2821 ctcccagtat tagtgggtta attcctcaat tttcaactct ggcaggaatt tggtaggtaa
     2881 attgataatc caagattatc taaactgttt gtaatctctt ctgaaattgt tgaatcacct
     2941 gaataatacc agtgactata tgtattcaac gtggttgact tatcagcgtg tcctaatcga
     3001 tatgaaacat ataaagtatc cttatgtaac acatttataa gataagatgc atgactatgt
     3061 cttaatcctt tccccgtaat ttctggtaca ccagtaatct tagcatgcct ttttattatc
     3121 ctagaaattg tagacttaca caagggggca ccaaaacgtg atattacata atctttatca
     3181 tcattcttga tttgaacttc tcgccatttt ttaagtattg taatcgtaaa atcatctaaa
     3241 tcaattttac gatcccctgc tactgtcttt gtttgttgtt taacatacca tacaccgttt
     3301 ttatctttct caattgtcga atgaacatga atccatttac gttcaaaatc aatatcttcc
     3361 catttaagag caataccctc actaactctt aaaccagtca taaaatataa ccagatcgtc
     3421 atgcaattat ggagtccctc atactcacta atatcaaaag agtttattac cttcttaaat
     3481 tcatcaaatg tccaaaattt tgtatcaggg tactttcctc taggattatc taatccttta
     3541 aaaggaactt tatcaatata acccaatctt tctgcatagc ccagacatgc tttaaatcta
     3601 ccacatattt ttagcataat tactagaata cttgtctata atagctaagc gaaatctttc
     3661 acagtcaata gtactaatat ctgataattt tttactacca aattgcttaa taaataactg
     3721 atgatgtggt aaagcggtct gataagttac agattgaact ttttgttgat aatattttaa
     3781 ataaatctct tccat
//
LOCUS       DAVKDZ010000130         3751 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000130 DAVKDZ010000000
VERSION     DAVKDZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.132"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            87..854
                     /locus_tag="VWZ30_002099"
     CDS             87..854
                     /locus_tag="VWZ30_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU9335744.1"
                     /translation="MKTLLIGDLHLKSQLILPIVDKIIQTHNIKRIIFLGDYVDLHGQ
                     TNNIQLYAKDLTFLYSWKIDKEQNGIDVINLIGNHDAYYLLGEQAPFSIQNLEVFFAV
                     KELLQDLKLQVAYQLDDYLVSHAGFNLIFDPKEWHFNLYTKEHEEELDILAYTIGPMR
                     GGKALGGSPLWAHFRELELLPNQDFPKQIVGHTPKESIDISKNVIGIDTFSLYIDKDN
                     KYQFIGNGDLLVYDQGHFEVISTDWATDKILKQLPRL"
     gene            1040..1819
                     /locus_tag="VWZ30_002100"
     CDS             1040..1819
                     /locus_tag="VWZ30_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEU9335745.1"
                     /translation="MTSYNKKIVRTSSYIEIWEYEKPIFSKGKSILDTDIKSKEKLQR
                     RTFDELTPEEQEQRLERMKKTRLEAKWNLLRLVDCNYDDNTSFLTLTTKENISDRNHF
                     KDLLKTFIKRFNYHIFNTKKSKLKYLTVLEKQKRGAWHAHILLFSVPYVPHRQLLELW
                     EHGAVRINKVDVDSKENRGRYVTKYFEKGIGQELLENFGKQAYFSSRNLKKPDEDRFY
                     TYEDFNYDSSVVLYETEYISKVYKDGQYFDNHVKYKKIKLD"
     gene            1836..2039
                     /locus_tag="VWZ30_002101"
     CDS             1836..2039
                     /locus_tag="VWZ30_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEU9335746.1"
                     /translation="MIATVTKNDLVALGFSEGTSKRIIRQGKELLITRGFTVYQNKRI
                     GTIPATIVSELLGFDVQNSTLKE"
     gene            2043..3176
                     /locus_tag="VWZ30_002102"
     CDS             2043..3176
                     /locus_tag="VWZ30_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU9335747.1"
                     /translation="MAIRKGKDGNWIVDISNGFDPITLKQRRIVRKGYKTKKEAIEAE
                     HYLRSVELKERFYGAKITIPILYELLKEEDSINHRKASYINTQDNNYNKHIKDYFSMV
                     YNVGKLTYEDIYQFREHLRQKVAKNSDKPLSTNTINKIMILLKKIFDVGLRKGYYTSN
                     PANLIKKLPIEKTKMQFWTVKEFQHFLTLFETEEYNIKLLFTVLFFTGLRLGEALALT
                     WQDIDFTSNTIHITKSVYVNKGISHVSTTKTKAGTRRIIINKKLSHELQQWQQKQKHL
                     LEQFTSDSMTLQVFQGSPITITKNAVEKQYKKILERDSTLKKIRIHDFRHSHASLLIN
                     QGEDYLVVKERLGHASITTTIDTYSHLYPSKQKDLADKLDDLL"
     gene            3217..3654
                     /locus_tag="VWZ30_002103"
     CDS             3217..3654
                     /locus_tag="VWZ30_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU9335748.1"
                     /translation="MDTSQVIKAHEVAKKAHFGQIDRAGIDYIKHPETVDSFVATDEE
                     KAAAYLHDVIEDTSLTLLDLKKEGFSKNIIEAVDILTKKKGQDYQSYLNLVKKNELAR
                     VVKLADLRHNSDLTRLPLITEKDLERNKKYSSAIRFLRAKKKS"
BASE COUNT         1465 a          610 c          619 g         1057 t
ORIGIN      
        1 taattctatt tatctatcac cgaaacaatg tgctataact taattcgatt cttagactaa
       61 tatcaaaaat aaagagggga atatatatga agacgttatt aattggtgat ttacacttaa
      121 aatctcaact gattttaccc attgttgaca aaattatcca aacacataat attaagagaa
      181 tcatttttct tggtgattac gttgatttac atggtcaaac taacaatatc caactttatg
      241 ccaaagacct cactttttta tactcttgga aaatagataa agagcaaaac ggaatagatg
      301 taattaatct tataggtaat catgatgctt attatttact gggagaacaa gctcctttct
      361 caattcaaaa cctagaggtc tttttcgcag ttaaagaatt gttgcaggat ttgaaattac
      421 aagtagctta tcaactagat gattatttag tcagtcacgc tggtttcaat ctgatatttg
      481 acccaaaaga atggcatttt aacctctata ccaaagagca tgaagaagaa ttagatattc
      541 tagcttatac aataggacca atgcgtggtg ggaaagctct tggggggtct cctttatggg
      601 cacattttag agaattagaa ctgcttccca accaagactt tcctaaacaa attgttgggc
      661 atacccctaa agaatctatt gatatttcta aaaatgttat tggtatagat actttttcat
      721 tatatattga caaagataat aaatatcaat ttattggtaa tggagattta ctcgtttatg
      781 accaaggaca ttttgaggtc atttcaacag actgggcaac agacaaaata ttaaaacaat
      841 taccacgact ataaatttaa tagctacttc tcatcatgta cctaagcaaa actgccgact
      901 aagtcggcaa gctggaagtg tgtttagcgg agcggtgctt agtcacatga tagaagtagg
      961 gaggagcaat gctcctctta gtttatttat tatatagaca atcttttggc gaaagccaac
     1021 ctaaaaggaa ctatttttta tgacctcata taataaaaaa atagtccgca cttcttccta
     1081 tattgaaatt tgggaatatg aaaagccaat attttcaaaa ggaaaatcca ttttagatac
     1141 tgatattaaa agcaaagaaa aattacaacg tagaacattt gatgaactta cacctgaaga
     1201 acaagaacaa cgtctggagc gtatgaaaaa aactcgctta gaagcaaagt ggaacttact
     1261 ccgattagtc gattgtaatt atgatgacaa tacttcattt ctaaccctga ccactaaaga
     1321 aaatattagc gacagaaacc acttcaaaga cttactaaaa acttttataa aacgctttaa
     1381 ctaccacatc tttaatacca agaaaagtaa attaaaatat ttaacagtat tagaaaagca
     1441 aaaacgtgga gcatggcatg ctcatattct gcttttcagc gttccttatg taccacaccg
     1501 tcaactcttg gaactttggg aacatggagc agtacggata aataaagttg atgtagacag
     1561 caaagaaaat cgcggtcgat atgtcacaaa gtattttgaa aaagggattg gacaagaatt
     1621 gttggaaaac ttcggaaagc aagcctattt ttcatcacgc aacttaaaaa agcctgatga
     1681 agatagattt tatacttatg aagattttaa ttatgatagt tcagtcgtac tatatgaaac
     1741 ggaatacatt agtaaagtct ataaagacgg tcagtatttc gataatcatg taaagtataa
     1801 gaaaataaaa ctagattaat aaaggagtaa gttacatgat agcaactgtt actaagaatg
     1861 acctagttgc ccttggtttc tctgagggaa catctaaacg tattatccgc caagggaaag
     1921 agctactgat aacacgaggc tttacagtct atcagaataa acgaattggt actatacctg
     1981 ctactattgt aagcgaatta ctcggttttg atgttcaaaa cagtaccctc aaggagtaaa
     2041 atatggcaat tagaaaaggt aaagacggta attggatagt tgatattagc aacggttttg
     2101 acccaataac actaaagcaa cgtagaattg tcagaaaggg ctataagaca aaaaaagaag
     2161 ctattgaagc agaacactac ttacgaagtg tagagttaaa agaacgcttc tatggggcta
     2221 aaataactat ccccatacta tatgaactac taaaggaaga agatagtata aatcatcgaa
     2281 aagcaagcta tatcaataca caagataata attacaacaa acacatcaaa gattattttt
     2341 ctatggtata taatgtcgga aaactaacct atgaagacat ctaccaattt agagaacatc
     2401 ttaggcaaaa agtagctaaa aattctgata aacctttaag cacaaacact atcaacaaaa
     2461 tcatgatttt actcaaaaag atttttgatg ttggtttgcg aaaaggctat tacacctcca
     2521 atccagcaaa tttgataaaa aagttgccaa tcgaaaaaac aaaaatgcag ttttggacag
     2581 tcaaagaatt tcaacatttt ttaaccttgt ttgaaactga agaatataat atcaagttac
     2641 tctttactgt tctattcttt acaggactac gacttgggga agcattagca ctaacatggc
     2701 aagacattga ttttacaagc aatacaattc acattacaaa atcagtttat gtaaacaaag
     2761 gaataagtca tgttagcaca acaaagacta aagctggaac aagaagaatt atcattaata
     2821 agaaattgag ccatgaacta caacaatggc aacagaaaca aaaacaccta ttagaacaat
     2881 ttactagtga tagcatgacc ctacaagtgt ttcaaggctc acctattaca attacaaaaa
     2941 atgcagttga aaaacaatat aagaaaatac ttgaacgtga tagcacacta aaaaaaatac
     3001 gcatacacga ttttaggcac tcacacgctt ccctgctgat taaccaaggg gaagattatc
     3061 tagttgtaaa agagcgacta ggacatgctt ctataacaac cacgattgat acctactctc
     3121 acttgtaccc aagtaagcaa aaggatttag ctgataaact agatgattta ctataaagta
     3181 attgagtttt ataaatggaa agtttataat taaatcatgg atacatcaca agtaataaaa
     3241 gcacatgaag tagctaaaaa agcacacttt ggacaaattg atagagcagg aattgactac
     3301 attaaacacc ctgaaacagt agatagtttt gtcgcaactg atgaagagaa agctgccgcc
     3361 tatctacatg atgtaattga agatacttca ctaaccctac tagacctaaa aaaggaaggt
     3421 ttttcaaaaa acattataga agccgtcgat atactcacaa agaaaaaagg acaagattat
     3481 caatcttatc tcaatctagt aaagaaaaat gaactcgcta gagttgtaaa gttggcggat
     3541 ttacggcata attcagattt aacaagacta ccattaatta ctgaaaaaga cttagagaga
     3601 aataagaaat acagttctgc tattagattt ttaagagcaa agaaaaaaag ttagtaacta
     3661 aactataaaa ttttataaag agaaaacaaa agacaaggct tataaacttt gtctttatta
     3721 ctatactggc ggtcggtctc gaactggcat a
//
LOCUS       DAVKDZ010000131         3562 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000131 DAVKDZ010000000
VERSION     DAVKDZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.133"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..>3562
                     /locus_tag="VWZ30_002104"
     CDS             <1..>3562
                     /locus_tag="VWZ30_002104"
                     /inference="COORDINATES: protein motif:HMM:NF020018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335749.1"
                     /translation="PIIRSNSDTLLSTAVEDAVTVTNKENVEKQGNLPTGIGKHTINA
                     KVTYKDQSEEMVEIPYTIKPDAPSISTSIGTAGKNSVTVNNVTPGTTVVVYDMTNPNN
                     PIELGRKDVSGATADAAQNGVTVSTNATLRKDTPIAAEVIYKPTVAAERISSDKSSSL
                     VVKEGLAVNSIHAVKGENYTGDLASRIHYNDGQDTGLPAGSTVEWKNNQAPDYNNVGT
                     SRYTLVVNVPNQGTTEIEVPVHVYPTASLKKASYTNKQGTLSNGTDASKYVQFEGSAD
                     TPNNVTVRWKDGVPDVSTVSADRKAKIEVVYPGNASATDTVVKELEVSLPTYHSTAKS
                     TEYTRTIGEAYASTDASDYVETTPNTPRGTEYAWKTDETGNQAYGSSTWGGANGDWLG
                     KKTNKVKVYYPNADGGNEKSEVLAEETEEITFITKPAKPSITSDLTWASGTRTTVEVG
                     NVTSGTRVVLYDEQGNELGHTDVAKGANYATPTTASITPTKALPTGKVYVKTIYMPDT
                     ADQRVESEKSDEATAKTNTLTAKGIIQTLAGPGNIGGVGTLDAATLGKLLRQENGGTD
                     FTGATAEWKDKTNLEKGNGGSRVEHLLVQLKGHSEKQDVAITVTSLTPPSAKAVLKSK
                     GADLTNDNLADYVTAEGQGTLSWEGNPAKVEVGQALPRIKVTYPTTGVAITDITDQYV
                     DAKVYSLEANSNAKTRVTVGDAFDPSAGDYVQTVANTADLPRTGVSHAWKDGNKPTSA
                     TVGKATYTVVTSFGNDADVPAELRGQDVETQVEVTVLSTKPSKPEASQNRTDLSITVV
                     VGKDDATKAEITFKDELDQEHTVSFTKGANGQWDKDDANSQPTVQIINNADGTATVHM
                     TGGTAKAGSTVVTKQQKADSDFSDISELVVKEHLKGATVTTKDDGSVEVVVPKEADTA
                     TVSYVPEGQTQAKTVTITKGQDGNWTGPADSDLVIKKDDATGRVTVTVPADKVADGST
                     VTGRVDSATGLSPEVEVKGKAPQPSEFSHHVRDNGDEVITLPQNADSVTLNYPVSDAN
                     VKTVTISKGQDGTWTAPADSDLVVKKDDATGSVTVTVPADKISGNRTITASAKAGSGD
                     GESKERTFTTTVVERQAPTVSEVTVAANGTLSAEQINAAVTAPKKVSAVAAEALKQVA
                     AGSNTEIPVTVTYADGSTETVQLAVYSKQVTPSTVGFQQK"
BASE COUNT         1250 a          754 c          864 g          694 t
ORIGIN      
        1 cgccaatcat tcgctcaaac agcgacacgc ttctctcaac agcagtagaa gatgcagtaa
       61 ctgtaaccaa taaagagaat gttgaaaaac aaggaaacct tccaaccgga attggaaaac
      121 ataccatcaa tgccaaggtt acttacaaag accaaagcga agaaatggta gagattccat
      181 atactatcaa accagatgcg ccaagcatta gcacaagtat cggtacagcg ggcaaaaata
      241 gtgtgacggt taataatgtg actccaggca cgacagtagt agtgtatgac atgacaaacc
      301 caaacaaccc gattgaattg ggacgcaagg atgtgtcagg tgctacagca gatgcagcgc
      361 aaaatggagt gacggtttca accaatgcga ctctaagaaa agatactcca atcgcagccg
      421 aagtgattta caaaccaaca gttgcggcag agcgtatcag ttcagacaag agcagtagct
      481 tggtagtcaa agaaggtttg gcagtcaaca gcattcatgc ggttaaagga gaaaactata
      541 caggtgacct agcttctcgt atccactata acgatggaca agacacaggt cttccagctg
      601 gttcaactgt tgaatggaag aacaaccaag cgccggacta caacaatgta ggaacaagtc
      661 gttatacact tgtagtaaac gttccaaatc aaggaactac agaaatagaa gtaccagtcc
      721 atgtttaccc aacagcaagc ttgaagaagg ctagctacac caataaacaa ggtactctgt
      781 caaatggaac agatgcaagt aagtatgttc aatttgaagg tagcgcagat acgccaaata
      841 acgtaacagt acgttggaaa gatggtgttc cagatgtctc tacagtctct gcagaccgta
      901 aggctaagat agaagttgtc tacccaggta atgcttcagc aacagataca gttgtgaaag
      961 aattagaagt atcacttcca acttaccatt caacagctaa gtctacagag tatacacgta
     1021 ccattggtga agcttacgct tcaaccgatg cgtcagacta tgtagaaacc actccaaaca
     1081 caccaagagg aacggaatat gcatggaaga cagatgaaac tggcaaccaa gcctatggct
     1141 catcaacttg gggaggggca aacggtgact ggctcggtaa gaagaccaat aaggttaaag
     1201 tttactatcc aaatgcggat ggtggcaatg agaaatctga agtcttggca gaagagacag
     1261 aggaaattac tttcatcacc aaaccagcca aaccaagcat tacaagtgac ttgacatggg
     1321 caagtggtac tagaacgact gtcgaagttg gcaacgtgac aagcggtaca cgggtagtct
     1381 tgtatgatga gcaaggtaac gagctcggac atacagatgt agccaaagga gctaattacg
     1441 caactccaac cacagcaagt atcacaccaa ccaaggctct accgacaggt aaagtctatg
     1501 tgaagacgat ttacatgcca gacactgcag atcaacgtgt agagtcagag aaatcagatg
     1561 aagcgactgc taaaaccaat accctaacgg ctaaaggaat tatccaaacg ctggcaggcc
     1621 caggtaacat tggaggagtc ggcacgcttg atgcagctac tttaggtaag ttgttgagac
     1681 aagaaaatgg tggtacagac ttcacaggag cgactgcgga atggaaagat aagacaaact
     1741 tagagaaggg caatggagga agtcgagtag agcaccttct tgtccaacta aaaggacaca
     1801 gtgagaaaca agatgtggct atcacagtca caagtttgac tccgccaagt gccaaagcag
     1861 tcttgaaatc gaagggtgca gacttgacaa atgataactt ggcagattac gtaacagcag
     1921 agggacaagg taccctaagt tgggaaggta atccagccaa ggtagaagtg ggtcaagctc
     1981 ttccacgtat caaggttact tacccaacaa caggtgtagc tattactgac atcactgatc
     2041 aatatgtgga cgccaaagtc tacagtctcg aggctaacag taacgccaag acaagagtga
     2101 cggtgggaga cgcctttgac ccatcagcag gagactatgt tcaaacagtc gctaatacag
     2161 cagacttacc aagaactggc gtcagtcacg catggaaaga tggcaacaaa ccgacatcag
     2221 cgactgtagg aaaagccacc tacacagttg ttacaagttt tggtaatgat gcagacgtac
     2281 cagcagaact tcgaggacaa gatgtagaaa cgcaagtaga agtaacggtt ctctcaacca
     2341 aaccaagtaa accagaggcc agccaaaacc gtacggattt atcgataaca gtagtagtcg
     2401 gaaaagacga tgcgacgaag gcggaaatca cctttaaaga tgaactggat caagaacaca
     2461 cggtcagctt cactaaggga gccaatggtc aatgggacaa agatgatgcc aatagtcaac
     2521 caacggttca aatcatcaac aatgcagatg gaacagcgac agttcatatg acaggcggca
     2581 cagctaaagc aggcagcact gttgtaacga aacaacaaaa agccgattca gactttagtg
     2641 atatcagtga gttggttgta aaagaacact taaaaggtgc cacagtaact actaaggacg
     2701 atggttcagt agaagtggta gtaccaaaag aagcagatac tgcgacagtc agctatgtcc
     2761 cagaaggaca aacgcaagcg aaaacagtga cgattaccaa aggtcaagat ggcaactgga
     2821 caggtccagc cgattcagac ttagtgatta agaaagatga tgcgacaggt agagtcacag
     2881 tcactgtacc agcagataaa gtagccgatg gcagcacagt tacaggaaga gtagacagtg
     2941 ctactgggtt aagtccagaa gttgaagtga aagggaaagc gccacaacca tctgaattta
     3001 gtcatcatgt tcgagacaac ggcgacgaag tgattacctt gcctcagaat gcggacagcg
     3061 tgacgcttaa ctatccagta agcgacgcga acgtgaagac agttacgatt agtaaaggtc
     3121 aagatggcac ctggacagct ccagccgatt cagacttagt tgttaagaaa gatgatgcga
     3181 caggcagcgt caccgtcacg gtaccagcag ataagatttc gggtaaccga acgattacag
     3241 caagtgctaa agcaggaagt ggcgatggag aaagtaaaga acgtaccttt acaacaacag
     3301 ttgtagagcg tcaagcacca actgtttcag aagtgacagt agcagcgaat ggcacactaa
     3361 gtgcagaaca gatcaatgcg gctgttacag ctcctaagaa agttagtgcg gttgcggcag
     3421 aagccttgaa gcaagtagca gcaggcagta acacagagat tccagttaca gttacctatg
     3481 cggatggctc aacagaaacc gttcagttgg cagtatactc taaacaggta acaccaagta
     3541 cagtcggctt ccaacaaaaa ga
//
LOCUS       DAVKDZ010000132         3551 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000132 DAVKDZ010000000
VERSION     DAVKDZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.134"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            267..362
                     /locus_tag="VWZ30_002105"
                     /pseudo
     CDS             267..362
                     /locus_tag="VWZ30_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(615..1235)
                     /locus_tag="VWZ30_002106"
     CDS             complement(615..1235)
                     /locus_tag="VWZ30_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9335750.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1235..1717)
                     /locus_tag="VWZ30_002107"
     CDS             complement(1235..1717)
                     /locus_tag="VWZ30_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU9335751.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGNKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYHIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1717..2559)
                     /locus_tag="VWZ30_002108"
     CDS             complement(1717..2559)
                     /locus_tag="VWZ30_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEU9335752.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2654..3454)
                     /locus_tag="VWZ30_002109"
     CDS             complement(2654..3454)
                     /locus_tag="VWZ30_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEU9335753.1"
                     /translation="MRKQIHPLILFSFLGIMMFILIINRPLNDNQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFFES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
BASE COUNT         1097 a          638 c          721 g         1095 t
ORIGIN      
        1 ccaaaagtta gattttttct gtctaacttt tggggtgcag ttcatttaac acctgataat
       61 atgcgttttt tattttaaag actttttgac cagcctcatt tttttaactt gatactcagt
      121 gaaaagcaaa gattaaacta ggaagctagc tgtaggctgt tcaaagaaca gctttgaggt
      181 tgcagataaa acttgtgagg taaccaacat atataatgtg aagctgacgt ggtttgaata
      241 gattttagaa gagtatgagt ttggaagttt taatggataa tgcaagattc catagaatgg
      301 gtaagctaga gttcttatgt gaagagtttg ggcataaact tttacctttt cctccctact
      361 catcttagta tagaaaagtg aatctgaaat agtacataac tgcttctaaa acattcttat
      421 aaattgattt aaattctcaa atcatattat tcagttctta tttcattttg ctctacaatc
      481 ctgttgagaa gacacgtgtt catatcaaaa aggtattggc aagttgcaat acctttttac
      541 aaggttcttt tgtcttattt ttgtttcaac tgactatatc tcctatggtt ctagttcaga
      601 aggctaggct ataattatga ttgataagaa gtatcattcc aagtattgag agtgaatgtt
      661 tcaaaatcat gggtttctat aatggtcagg ctggcatttg ctagaccgcc atctttacga
      721 agaagtggtt ctttatagcc taggagagta cgaagactgg cagtaagatt ggcgccgtgt
      781 ccgacaatta gaatacgctc agctggacta tcttttaatg atttgataaa ttggatggtc
      841 cgctgagttg tactatagag ggattcggct ccgaacattc gagtgtcaaa ttgagcaaga
      901 tttgaacgaa aagcctggat ttgttgcggg taaatagctt ccagggttgc aattttcaaa
      961 ccttctaact tcccaagttg ccattcacgg agattaggaa cgatttctaa agaacagggg
     1021 gtatggagtt gactttggat aatctcagca gatttgaccg ctcgaggtaa atcacttgaa
     1081 taaatctgat caaaaggaat ttccttgaga tactggccaa gtcgttttag ggtttcaatg
     1141 gattcaggaa gaagaggaga atcaccgcta gcaccttgaa aacgaccttc ttggttccag
     1201 agggtacgac cgtggcggac aaagtagagt ttcattactt gatgtcctcc aaaatatcta
     1261 caaagtctgc ctttacaaag ctagccaagt cttgtggcgc gacgataatg ctgtgtccga
     1321 cttcgcctgc agagacaatc attcgatcca aatctagagc aattttatcg ataaaaatgt
     1381 gataattgtg tttctgacga attccgacag gattattggc tccatgaatg taaccagttg
     1441 ttttttctaa gtccttttgt ggaatcatgc tcactttttt attgccagaa attttagcta
     1501 gtttcttttc agacaagtgc tgagtgatag ggacaattcc gataatcggt ccggtcttgt
     1561 ttcccaaaag cgccaaggtt ttgaaaatct gatctcgttc ataaccttga ggaagctctc
     1621 cttctagggc attgatttga atcccctgat gagggatagc tgctttagat aggatttgtt
     1681 ccaccaatgt ttttttgatt ttaacttttt ttgccattat ttatatttat cctccaattg
     1741 actcatccaa ataccaagcc agattcccag cgcaaagaag aaggcgatga tgacataacc
     1801 gacaagtgaa agtcctgtgt attggatact ttcagcgttt cctgcatttg gaattaagat
     1861 caaaagggta cttgatagga cgataccgat gatgaaatga tagacgcgtg agtggtagtt
     1921 gtttaaggca taatccatca attttgaaaa aacgatgaga gttgcacctg caccaattcc
     1981 aatcggaaag aaggttccca agagatcaaa agttttaaaa ccagtcaaca taggagcata
     2041 gagtcccaaa atcaaaagta aattcgatgg gctgaggcca ggaaccaaga cgccaagggc
     2101 caatagtgca cctgctagga cgaagttaag aaagctggcg cttaaggttc caacgacaaa
     2161 atttaaggca tagagtccta atccagaaat gataaaggtt gtccataacc aagctaaatc
     2221 aatcttgtct cggtcagatt ctcgagttga ttctttgagg aggctaggaa ctgtaccgat
     2281 aatagctccc gcaaagctcc ataatacaaa gacctgataa ttttcaagca ggtattcaat
     2341 cgggtaggaa aataagccga ttcccagaag cataccgatg gcaactggaa taaagtacaa
     2401 aacattttct ttaaggtctt taaagggatg ggccagaaag ccaatcattc gttcatagat
     2461 tcctaagatt gctgctagaa ctcctccgga aattcccggt aggataaatc caagagcaat
     2521 tacaatccct ttaataacgc gtgctaacca tgagagcata tatttcctcc aactatttct
     2581 atttcaaaaa atccttacta tattgtatca caatcagaca caaaaagaaa aagcaaatga
     2641 taaacaaatg cttttaaggt tttaaaaaga gttttcgaaa ggtttcttct ttatttttta
     2701 agggagagat aacgttgata tctaaatcgt ggtcaaagcc ggcaattttt cctttagatg
     2761 tgtattggtg aatatcataa tctaaatcag ttttaggact gctctcaaaa aatcctgagt
     2821 ctgagccgta ggacggaatc caaacagagg taaacttgcc tgtatcaata ctgtgttctt
     2881 ccatgaagta gacaccaacg tagatgccga tgtttttagc acctagtgat gctagttttg
     2941 ctcgaaaatt ttcgacacct tcgttcatat tagacatggt tttgtcttcc acgtcaagcc
     3001 aatagtaact agggctgtaa ggagaggcag cattgtagaa aacttcggca gccttttcca
     3061 tttcttggac actttttcca gctacataag cgtagacagc aactgggaca ttccgctttt
     3121 gaagttcagt gatatgactc ttataggcct tgtctattcc attgataaat gaagcatcat
     3181 tttcttttgt cgtttgagca ccactgtgaa cacgaacaat agcacctgaa atattttgtg
     3241 agagggcatc gtagttgatt tcctcaggac gctgccagcc agagaggtca ataatcggtt
     3301 tgtctaagtg tttcaaagcc tgtgcttcaa tctgtgctat attggatttt gttttaaacg
     3361 attggttgtc attaagtggg cgattgatga ttaaaatgaa catcataatc cccaaaaaac
     3421 taaataaaat aagtggatga atttgttttc tcatatctta taattctacc ctaaaaatca
     3481 aaaaaaatca aaaaaatggg ttaaggaaga gactttagag cattttttca ttcaagagtg
     3541 cggaatgatt t
//
LOCUS       DAVKDZ010000133         3534 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000133 DAVKDZ010000000
VERSION     DAVKDZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.135"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(35..424)
                     /locus_tag="VWZ30_002110"
     CDS             complement(35..424)
                     /locus_tag="VWZ30_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006268423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335754.1"
                     /translation="MQTFNYDDFKNEKGIVSFSEAEQIYSSLLNSSNQLDKEFQEEWT
                     TFVLLCVEYASARGKWLTLSREEKLANDESRTVTHNKVIYQLKILKGLASEQGNDVAW
                     FEQFNDDRKRIGDFACYVAYIYALNAR"
     gene            complement(458..772)
                     /locus_tag="VWZ30_002111"
     CDS             complement(458..772)
                     /locus_tag="VWZ30_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335755.1"
                     /translation="MKLNDFLKPELLGNRFFAVKGYTEVLDRETNKPVALRLNVSIQD
                     ENSDFFMEMIQVKINTLSPTASVQEMANNKTCPVSLKDLNVGQFNGNLWFACSDVLPV
                     TK"
     gene            complement(870..2234)
                     /locus_tag="VWZ30_002112"
     CDS             complement(870..2234)
                     /locus_tag="VWZ30_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEU9335756.1"
                     /translation="MRMLNQTIRVKSKYPYLIRPRNITITRLSIQVFLFLLLVSSWLA
                     AIPFIARLVFSMFFISLLFLVERFFHSDVIEKLRNKLNIREALFYMLISLTLYDDIDN
                     EVVDTAVLYFDVSENDKVIICVPLFGNRYLKTLKNLEEYLCPTLGLSLIFKKEEIDKI
                     VYVLGKIEEIEQYVFNTNTLTREFFKDIPSPIIKLSNTQQFSLKSNTNIGIYGRTGTG
                     KTIALQWYLFNALAKGCGTVDGTYLGIVDGKGADLYALGKLLEEEIGNQVTVGTSPTS
                     LAKLSREFVEIMNERFEVIKQNSSLNADAYDLGMIPNFLFVDELASIRDSCCGSSKQA
                     KELWNEILQNLGLIARKGRQAGCHLVLSTQDPNAENIPVELRNQISAVLYLGGPGADR
                     LKMAFSMCELENVPTVSDRKGEALFYADGLNSVEPVLTIVPFVDVKTKQEFLQIVKNI
                     LPNY"
     gene            complement(2197..2439)
                     /locus_tag="VWZ30_002113"
     CDS             complement(2197..2439)
                     /locus_tag="VWZ30_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335757.1"
                     /translation="MKSLYKYLFYLFFIGFAGTLCYVLLLTNAFSPIFEAATILMEII
                     VVIFLIICIDLIASPTGSAEENIKNENVKSNNPCEE"
     gene            complement(2501..2782)
                     /locus_tag="VWZ30_002114"
     CDS             complement(2501..2782)
                     /locus_tag="VWZ30_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335758.1"
                     /translation="MFKELIEFVNSSTEGQFKAFQVKANAITGDVIISQNVTPITDAL
                     KNRLGLKTVQTSLARKLAYASTRRHYKDGTTMMEDILAGKTRRHANSYI"
     gene            2936..3412
                     /locus_tag="VWZ30_002115"
     CDS             2936..3412
                     /locus_tag="VWZ30_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335759.1"
                     /translation="METRNEKFRRLSEARMTKVFSILNILRNQSDKSKYSFSEADIKE
                     LFGALEQKGEEIKEFFTSPITIKTVNLKQEFNYSSTDTSNDKEVYFKKLSTARVEKIF
                     SLMNLLTNLSNKSNYSYNDWEIEELFTAYDEEVKKCKVFFEEKRTVFKYSEQAIKY"
BASE COUNT         1216 a          613 c          563 g         1142 t
ORIGIN      
        1 agagtaatat atagctataa tatcctctaa ttgtctatcg agcatttaag gcatagatat
       61 aagctacata gcaagcaaag tcgccaatac gttttctgtc atcgttaaat tgttcaaacc
      121 aagcaacatc attgccctgt tcacttgcta aaccttttaa aatctttagt tgataaataa
      181 ctttattgtg agtaacagtt ctagattcat cattggcaag tttctcttct cttgagagtg
      241 tgagccattt accacgtgca gaggcatact ctacgcatag aagtacaaat gttgtccatt
      301 cttcttgaaa ttctttatca agttgattgg aagaattaag gagactagaa tagatttgtt
      361 ctgcttcaga aaatgaaaca atcccctttt cattcttaaa atcatcgtag ttaaaagttt
      421 gcattgtaaa actcctttat atattattta atcttagcta ttttgttaca ggtaaaacat
      481 cagaacaagc gaaccacaaa tttccattga attgccctac gtttaaatct ttaagactaa
      541 caggacaagt tttgttgttc gccatctctt gaacagaagc agtaggcgat aatgtattta
      601 ttttgacttg tatcatctcc ataaagaaat cagaattctc atcttgaatt gacacattaa
      661 gtcgtaaagc tacgggttta ttagtctctc tgtctaatac ttctgtatac cctttcacgg
      721 caaagaagcg attgcctagt agttcgggtt tcaaaaaatc atttaatttc atgataaatt
      781 cctttctttc ttacctcctg caatgcagtc ggttggctct ttttctctat tgaaaagaat
      841 tcctattcca acagagaaaa ataccgtcat tagtagtttg gcagtatatt tttaactatc
      901 tgtaaaaatt cttgtttggt ttttacgtca acgaatggaa cgattgtcaa tacaggctca
      961 actgaattta aaccgtcagc ataaaagaga gcctctcctt ttctatcgga tacagtagga
     1021 acgttttcta attcgcacat gctaaaagcc atcttcaatc tatcagcacc tggacctcca
     1081 aggtataaaa ctgccgatat ttgatttctc aattctactg gaatattctc agcattaggg
     1141 tcttgtgtgg ataatactaa gtgacaacca gcctgccttc ctttacgggc aatcaatcct
     1201 aaattttgta gaatttcatt ccaaagttct ttcgcctgtt tgctagagcc acaacaacta
     1261 tcgcgtattg atgccaattc gtctacaaat agaaaattag gtatcattcc taaatcataa
     1321 gcatcagcat ttagggaact gttctgcttg ataacctcaa aacgttcgtt cataatttct
     1381 acaaattccc ttgacaattt cgctaaagag gttggagaag tacccacagt gacttgattt
     1441 cctatttctt cttcaagtag cttccctaat gcatataagt ctgccccttt tccgtctaca
     1501 atgcctaagt aagtaccgtc aacagttccg caccctttag caagagcatt aaataaatac
     1561 cattggagag ctattgtttt acctgttcca gtgcgtccat aaataccgat attagtattc
     1621 gatttcagag agaattgttg ggtatttgat agcttgatta ttggactagg aatatcctta
     1681 aaaaactccc tagttaatgt attagtattg aaaacgtatt gttcaatttc ttctatcttc
     1741 cctaacacat atactatttt atcaatctct tcttttttaa aaattaagga caaccccaat
     1801 gtgggacaga gatattcctc taaatttttc aaggttttca gataacggtt cccaaataat
     1861 gggacacaga taataacctt gtcattctca gaaacgtcaa aatataaaac ggctgtatct
     1921 actacttcat tatctatatc atcatataaa gttaaactaa ttaacatgta aaacaaagct
     1981 tctctgatat tgagcttgtt tctcaatttt tctataacat cagaatgaaa gaacctctct
     2041 actaaaaaaa gaagacttat aaaaaacatg gaaaagacta gacgtgcaat aaatgggatt
     2101 gcagcaagcc atgaagatac caatagtaaa aaaagaaaaa cttggataga tagtctagta
     2161 atggtaatat ttcgaggacg aatcaagtaa gggtatttac tcttcacacg gattgtttga
     2221 tttaacattc tcatttttta tattctcctc agcagaacca gtaggggaag caatcaggtc
     2281 tatacagatt attaaaaaaa taacaactat aatctccatc aatattgtag cagcttcaaa
     2341 gataggtgaa aaggcatttg ttaaaagcaa cacataacag agtgtgccag caaatccaat
     2401 aaagaacaaa taaaaaagat atttatagag actcttcatg atgtcactcc tttctatttc
     2461 aatcaaaaga taaaggggag aagttagctc ccccagtcat ctagatgtag ctgtttgcat
     2521 ggcgacgtgt cttgccagct aagatgtctt ccatcattgt tgtaccatct ttatagtggc
     2581 gacgagtaga agcgtaagct aacttccttg ctaaagaggt ttgcacagtt tttaatccta
     2641 gacgattttt taaggcgtca gtgataggtg ttacattttg agagatgata acatctcctg
     2701 ttattgcatt agccttgact tgaaaggctt taaactgtcc ctctgttgag gaattaacaa
     2761 attcgataag ttctttaaac ataagaaccc ctcctttttg ggtattatat ttagaggata
     2821 tccctctata taggatatta tactacacaa aatcataaaa tgcaatattt gaaaaaaatc
     2881 tcgctatatg ataatatata ggataaagaa ataaaattta cttattggag aaactatgga
     2941 aactagaaat gaaaaattta gaagattgtc agaagcaagg atgactaagg ttttttcaat
     3001 cttaaatata ctgagaaatc aaagtgataa atcaaagtat tctttttcag aagctgatat
     3061 taaagaactt tttggggcgt tagaacaaaa gggcgaagaa ataaaggaat ttttcactag
     3121 tcctattact ataaaaacag ttaatctaaa acaagagttc aattattcct ctactgatac
     3181 aagtaatgac aaggaagtat actttaagaa actatcaact gcaagagtgg agaaaatttt
     3241 ttcattaatg aacctgctta caaatcttag caataaatca aattatagtt ataacgattg
     3301 ggaaatagaa gaactcttta ctgcctatga tgaagaagtg aaaaagtgta aggtattttt
     3361 tgaagagaaa agaacagtat ttaaatatag tgagcaagct atcaaatact aaaatgttac
     3421 taaaaagaaa aaatctatcg tttttgcgat agaaataatt tattgaaaat ttgattttac
     3481 tagatttatg aagttaagaa tatcatagaa tcaaactcaa aatccagtgt ccgc
//
LOCUS       DAVKDZ010000134         3526 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000134 DAVKDZ010000000
VERSION     DAVKDZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.136"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            59..1408
                     /locus_tag="VWZ30_002116"
     CDS             59..1408
                     /locus_tag="VWZ30_002116"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU9335760.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLIVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANSWVTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKSLMEGANTARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            1532..2992
                     /locus_tag="VWZ30_002117"
     CDS             1532..2992
                     /locus_tag="VWZ30_002117"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU9335761.1"
                     /translation="MLKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGVTPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVAIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     TDLETIFQMAKPFLEEAGCLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            3006..3440
                     /locus_tag="VWZ30_002118"
     CDS             3006..3440
                     /locus_tag="VWZ30_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9335762.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAIPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT         1179 a          683 c          740 g          924 t
ORIGIN      
        1 cttgaaaaaa acgaaggtaa gcgttatgat agaaaagaag aaatattgga ggaagaacat
       61 gtcacatatt aaatttgatt attcaaaagt tttagacaaa tttgttgcac cacatgaagt
      121 ggaatacatg caatcacaag taacagcagc agatgaattg atccgtaaag gaactggtgc
      181 tggtagcgac ttcttgggat ggttggacct tcctgaaaaa tacgaccgcg aagaatttga
      241 ccgcatcttg aaagctgctg agcaaatcaa gtcagacagc gatgttttga ttgtgatcgg
      301 tattggtgga tcttaccttg gtgcaaaagc agcaatcgac ttcttgaatc accattttgc
      361 taacttgcaa acaaaagaag aacgcaaagc gccacaaatc ctttacgcag gaaactcaat
      421 ctcatctact taccttgctg acttggtaga gtatgttgca gacaaagact tctcagtaaa
      481 cgtaatttct aaatcaggta caacaactga accagcgatt gctttccgtg tctttaaaga
      541 actcttggtt aagaaatatg gtcaagaaga agctaacaaa cgtatctatg caacaactga
      601 ccgccaaaag ggtgctgtta aggttgaagc agacgctaac agttgggtaa catttgttgt
      661 tccagatgat atcggtggac gcttctcagt attgacagcc gttggtttgc ttccgatcgc
      721 agcatcagga gctgacatca aatctcttat ggaaggtgcg aatacagctc gcaaagacta
      781 cacttcagac aaaatctctg aaaacgaagc ttaccaatac gcagccgttc gtaacatcct
      841 ttatcgtaaa ggctatgcaa ctgagatctt ggtaaactat gagccatcac ttcaatactt
      901 ctcagaatgg tggaaacaat tggctggtga atcagaagga aaagaccaaa aaggtatcta
      961 cccaacttca gccaacttct caactgactt gcattcactt ggtcaattta tccaagaagg
     1021 aactcgtatc atgtttgaaa cagttgtccg tgttgacaaa cctcgtaaaa acgtgcttat
     1081 tcctactttg gaagaagacc ttgacggact tggttacctt caaggaaagg acgttgactt
     1141 tgtaaacaaa aaagcaactg acggtgttct tcttgcccac acagatggtg atgtaccaaa
     1201 catgtatgtg actcttccag agcaagacgc tttcactctt ggttacacta tctacttctt
     1261 cgaattggca attgcccttt caggttactt gaatgctatc aacccatttg accaaccagg
     1321 tgttgaagct tataaacgta acatgtttgc ccttcttgga aaaccaggat ttgaagaatt
     1381 gagcaaagaa cttaacgcac gtctataata gaagaaaaga gtggtttgcc cactcttttt
     1441 actctcttta tccatagaaa ttggactcag ccaagacttg tgatataata tagaaagcaa
     1501 aaaggcagac gcctagataa taggagaaac tatgttaaaa gatatccgcg tacgttacgc
     1561 accaagtcca acaggactac tacacatcgg aaatgctcgt acagcattgt ttaactactt
     1621 gtatgcgcgc catcatggtg gaacatttct catccgtatc gaagatactg accgtaaacg
     1681 ccatgtcgag gatggtgaac gttcacaact tgaaaacctt cgctggttag gcatggattg
     1741 ggatgaaagt ccagaatcac atgagaatta tcgccagtct gagcgtttgg acttgtatca
     1801 aaaatacatt gaccaactat tagctgaagg aaaagcctat aaatcttacg ttacagaaga
     1861 agagttggca gctgaacgcg aacgccaaga agtagctggc gaaacaccac gctacatcaa
     1921 tgaatacctt ggtatgagtg aagaagaaaa agcagcttac atcgcagaac gtgaagcagc
     1981 aggggtcacc ccaactgttc gtttggctgt caatgagtca ggtatctaca agtggcatga
     2041 tatggtcaaa ggcgatattg aatttgaagg tggcaatatc ggtggtgact gggttatcca
     2101 aaagaaagac ggttacccaa cttacaactt tgccgttgcc atcgatgacc atgatatgca
     2161 aatctcccat gttatccgtg gagacgacca tattgctaac acaccaaaac agctcatggt
     2221 ttatgaagca cttggttggg aagctccaga gtttggtcac atgaccttga ttatcaattc
     2281 tgagactggt aagaagttat ctaaacgtga taccaacacc cttcagttta tcgaagacta
     2341 ccgtaaaaaa ggttatttac cagaagcagt ctttaacttt attgctcttc ttggttggaa
     2401 cccaggtggt gaagatgaga ttttctctcg tgaagaactc attaaacttt ttgatgaaaa
     2461 ccgcctcagc aagtcaccag cagcctttga tcagaaaaaa ctagactgga tgagcaatga
     2521 ttatatcaag aatacagacc ttgaaaccat ctttcaaatg gcaaaaccat tcttagagga
     2581 agcaggctgt ttgactgaca aggctgaaaa attagttgag ctctataaac cacaaatgaa
     2641 atcagtagat gagattatcc cattgacaga tcttttcttc tcagatttcc cagaattgac
     2701 agaagcagag cgcgaagtca tgacgggtga aacagttcca acagttcttg aagcattcaa
     2761 agcaaaactt gaagcgatga cagatgatga atttgtgaca gaaaatatct tcccacaaat
     2821 taaagcagtc caaaaagaaa caggtattaa agggaaaaat cttttcatgc ctattcgtat
     2881 cgcagtttca ggcgaaatgc atgggccaga attaccagat acaattttct tgcttggacg
     2941 tgaaaaatca attcagcata tcgaaaacat gctaaaagaa atctctaaat aaagaggata
     3001 caataatgga catctgggaa aagatgtacg aagaagcaca gaaactatac aatccacatg
     3061 aagtatcaga ctttgtttat gccaatcatg ttgtagctgc agtagaagca gaagatggac
     3121 aaatatttac aggattctgt atggagggaa cctgtggtgt tttccatctc tgcgcagaac
     3181 gggcggccct cttcaatatg taccaatttt caggacaaac taaggttaag aaagtattag
     3241 cctttcgaga taaaccacct tatggcggaa gttcagccat tccttgcggt gcttgtagag
     3301 aattcctttt agagcttaat gctgaaaata aagatgcaga attcatgatg gactataata
     3361 taagaaaaac agttaaagtc gcagaactaa tcccttattg gtggggagaa gaacgtgctt
     3421 ctaagtttaa tgagcgatag aagagtcagt ataattctgg ctctttttac tataagttga
     3481 aaaaatgaaa aaaattagaa aaagtagttg acaaagtttg aaaagg
//
LOCUS       DAVKDZ010000135         3445 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000135 DAVKDZ010000000
VERSION     DAVKDZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.137"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..501
                     /locus_tag="VWZ30_002119"
     CDS             <1..501
                     /locus_tag="VWZ30_002119"
                     /inference="COORDINATES: protein motif:HMM:NF021594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
                     /protein_id="HEU9335763.1"
                     /translation="QKNTLEKRLSKLEMIVKNNEAEYQKKMDIFNSYFKSVADRINKE
                     QPVLLYNPKTDEFPVSIGQLSEGTSTGTRKSLIAAYDIAYQLFAREINKATPKFIVHD
                     VLESIEGDDIRALVDEVESNQIQYISAILKEKLVSSGMSTEKQNEIIVLQLSMKDRLF
                     ERGNNC"
     gene            835..1197
                     /locus_tag="VWZ30_002120"
     CDS             835..1197
                     /locus_tag="VWZ30_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335764.1"
                     /translation="MRQDIKLIRKQFRITRQEDNQIKEMMREQQLESFSEFLRQNLLK
                     KESHEDVLIRWFTLWQSQKIEQISRDILQVTTLAEQSHQVTAEHLRIILTCVQELIAE
                     VNQASPLSQDFRDKYMGG"
     gene            1200..1565
                     /gene="mobC"
                     /locus_tag="VWZ30_002121"
     CDS             1200..1565
                     /gene="mobC"
                     /locus_tag="VWZ30_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEU9335765.1"
                     /translation="MENRYRTNLKKVFLTDQELAVLHDRIGQSRCKNFSAYARKVLLN
                     PNMTFLTIDTSNYEDLVFELRRIGNNINQIARTVNQNHILTPSDRELLVQGICQLIKE
                     VEKDFNIKCQQLKEFYGSH"
     gene            1552..3438
                     /locus_tag="VWZ30_002122"
     CDS             1552..3438
                     /locus_tag="VWZ30_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEU9335766.1"
                     /translation="MVVTKHFAVHSTKYRKSLIKYILNPEKTEQLKLISDFGMRNYLV
                     FPSYEEMVEMYEANLTNNDRLYDSRNDRQQIKQTKIHAHHLIQSFSPEDKLTPEEINR
                     IGYETIKELTGGNFRFIVTTHTDRNHVHNHILINSVDLNSDKKLKWDYVQERNLRMIS
                     DRLAKEAGATIIQPNRYSHEKFETYRKTNHKFELKQRLYFLLEHSNNFEEFLAKAPAI
                     NVEMDFSHKHARFLMTDREMKQVIRGKQLDKRQPYTEDYFREQFATRAIEQRLDFILP
                     RARDLSHLLEMAEQLNLTISPKKKHVTFTLTEKGRTISIENKKISTKHLYDVQFFEDY
                     FDQRESLTDPDLTNLVSDFEIYQEEQDKDKLPSEELLAAYKEFKEKRDQIQEFEVVLA
                     DHQIDKVVKDGLFIKMNYGVKKDGLVFIPNRNLDIHENEQGKSHHVFIRETAQFFIYN
                     KDSVDLNRYMRGRELICQLTHDSKHIPKRRRPTIASLKEKISEVNLLIELDINNQSYQ
                     EVKDELIKDIAQLDLTITNLQDKVAHLNKVAEILLNLNNSDPENRRLARYDYVKMNLT
                     AAINFEQVEQEISRSQQSLKDLIDKYEFKVRRLEKFVKNISSIYDYPFGGKTYTNNYQ
                     PDFE"
BASE COUNT         1164 a          658 c          694 g          929 t
ORIGIN      
        1 caaaaaaata ctttagaaaa aagactttct aaactagaga tgattgttaa aaataatgaa
       61 gcagaatatc agaaaaaaat ggatattttt aatagttatt ttaagagcgt tgcagatcga
      121 ataaataaag aacaacctgt cttgctttat aatcccaaga cagatgaatt tccagtttca
      181 attggccaac tatctgaagg tacaagtaca gggactagaa agtcccttat cgctgcatac
      241 gatattgctt atcaactttt tgctagagaa attaataaag caactcctaa atttattgtt
      301 catgatgttc ttgagagtat tgaaggtgat gatattaggg cgttagttga cgaagtggaa
      361 agcaatcaaa tacaatatat ttcagcaatc ttaaaagaaa agttagtatc ctctggtatg
      421 tcaacagaga aacaaaatga gataattgtt ctacaacttt ctatgaaaga tagattgttt
      481 gaaagaggaa ataattgtta gataaaatat aagtcttgaa ttttaaaagg aagttcaaga
      541 cttttttgtg ataacttttc caaatcatcc ttgttatcct aatgtcattc ctaagaaaat
      601 caaatcttga tttttcactg ggggtttggg ggcgaagcca ccatagtttc ttatcgtcgg
      661 ctgacaaacc tgtaaggttt tggttggctg acgattagat tttgggttat tgtggacaca
      721 ataactgagc tcgcaaaagc cgaaaaagaa ccttttgagg cttttaggaa gtgtactgac
      781 agtgtgaggc tgccttacat tgtcaggcaa gtaatttttt gaaaggataa aaccatgaga
      841 caagatatta agttaatcag aaaacaattc aggataacaa gacaggagga taatcagata
      901 aaagaaatga tgagagaaca acaattggag agtttctctg aattccttcg ccaaaattta
      961 ttgaaaaaag agtctcacga agacgttttg attcgctggt ttactctctg gcaatctcaa
     1021 aagattgaac aaatcagtcg agatatttta caggtgacta ccttggctga acaaagccac
     1081 caagttacgg cagagcacct acggattatc ttgacctgcg tacaggagct aatagcagag
     1141 gttaaccagg ctagtccact tagtcaagac tttcgggaca agtacatggg aggttaggga
     1201 tggaaaatcg ttaccgcacc aatcttaaaa aagtttttct gacagaccag gaacttgcgg
     1261 tcttgcacga ccgtatcgga cagagtcgct gtaaaaattt ctctgcttat gccagaaaag
     1321 ttttgcttaa tcctaacatg accttcctaa ccattgacac cagcaactat gaagacctgg
     1381 tttttgaatt gagacgaatt ggaaataaca tcaatcaaat tgcacgaacg gtcaatcaga
     1441 accacatctt aacaccaagt gaccgtgagt tgttggtaca gggcatttgc caactcatca
     1501 aggaagttga gaaggacttc aacataaagt gccagcagct gaaggagttt tatggtagtc
     1561 actaagcatt ttgcggttca cagcaccaag taccgcaaga gcctcatcaa gtatattctc
     1621 aatcctgaaa agacggagca gttgaagttg atttctgatt ttggcatgag gaactatttg
     1681 gtttttccta gttatgagga gatggtggag atgtatgagg ccaacctcac taacaatgat
     1741 aggctctacg attctagaaa cgaccgccag caaatcaagc agaccaaaat ccatgcccac
     1801 cacttgattc aatctttttc tcctgaagac aagctgacac cagaggaaat caaccgcatt
     1861 ggctacgaga ccatcaagga actgacaggt ggaaactttc gttttatcgt gacgacccac
     1921 actgatagaa accatgtgca taatcatatc ctgataaact ctgttgatct taactctgac
     1981 aagaagctca aatgggatta cgttcaagag agaaaccttc gcatgatttc tgaccgcttg
     2041 gccaaggaag caggggcaac gattatccag cctaaccgtt attcgcacga gaagtttgag
     2101 acttaccgca agaccaatca taaatttgaa ctcaagcaaa ggctttattt tctcctggaa
     2161 cacagcaaca actttgagga atttttggcc aaggctcctg ctataaacgt ggagatggat
     2221 ttttcccaca agcacgcccg ttttctcatg accgaccgag agatgaaaca ggttattcgc
     2281 ggtaagcaat tggacaagcg acagccctac accgaggact atttcagaga gcagtttgcg
     2341 acacgagcca ttgagcagcg attggatttt attttgccta gagcccgtga cctctctcat
     2401 cttttggaaa tggcagagca gttaaacctg acaatctctc ctaagaaaaa gcatgtgact
     2461 tttactttga ccgagaaagg tcgaaccatt tccattgaaa acaaaaagat tagcactaaa
     2521 cacttgtacg atgttcagtt ctttgaggat tattttgatc aaagagaatc cctaacagat
     2581 cctgacttaa ctaacctggt atcagacttt gagatttatc aagaggaaca ggacaaggac
     2641 aaacttccaa gtgaggaact cctagcagcc tacaaggagt tcaaagagaa acgtgaccag
     2701 attcaggagt ttgaagttgt tctagctgac catcagattg ataaggtggt caaggacggt
     2761 ctttttatta agatgaatta cggtgtcaaa aaagacggct tggtattcat accaaaccgt
     2821 aatctagata ttcacgagaa tgagcagggt aagtcccacc atgtctttat acgagagacc
     2881 gcccagtttt tcatttataa caaagacagt gtagacctca atcgttacat gagagggcgt
     2941 gaactaattt gtcaactcac tcacgacagc aaacatatcc ctaaacgtag gcgaccaact
     3001 attgctagcc tcaaagagaa aatctcagag gttaatcttc ttattgagtt agacatcaac
     3061 aaccaatcct atcaggaagt caaagacgag ttaatcaaag acatcgccca gctggatttg
     3121 accattacaa atctgcagga taaggttgct catcttaata aggtcgcaga aatcctgctt
     3181 aatctcaata actccgatcc agaaaatcgc cgactggcca gatatgatta cgtcaagatg
     3241 aacctgactg cggctataaa ttttgaacaa gttgaacaag agatttctcg ctcacaacaa
     3301 tctttaaaag acttgataga taaatatgaa tttaaagtta ggagactgga aaaatttgtt
     3361 aaaaatatta gtagtattta cgactacccc tttggaggta aaacatatac aaataactat
     3421 caacctgatt ttgaataaac atttg
//
LOCUS       DAVKDZ010000136         3259 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000136 DAVKDZ010000000
VERSION     DAVKDZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.138"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            9..1292
                     /locus_tag="VWZ30_002123"
     CDS             9..1292
                     /locus_tag="VWZ30_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9335767.1"
                     /translation="MVEIVRGGSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKE
                     KIKKSGLNKTRFVKKGTFLLTNSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLF
                     YILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLSEQQRIIEAIESALEKVDE
                     YAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFE
                     EGKIKKKDLDISIVSQGDDSSYYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIP
                     IYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIY
                     HENKNPYGWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELL
                     TKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI"
     gene            1349..2146
                     /gene="psrA"
                     /locus_tag="VWZ30_002124"
     CDS             1349..2146
                     /gene="psrA"
                     /locus_tag="VWZ30_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEU9335768.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLTFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            2157..2762
                     /locus_tag="VWZ30_002125"
     CDS             2157..2762
                     /locus_tag="VWZ30_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9335769.1"
                     /translation="MVKLTTCYFCDYGSYYGNIPMNWVVIKIKDIFSINTGLSYKKGD
                     LSINNKGIRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHI
                     GKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQA
                     LYNIPKTTLSELLIPLAPFEEQELITQKIEKLFEKVNQLWK"
     gene            complement(2710..>3259)
                     /locus_tag="VWZ30_002126"
     CDS             complement(2710..>3259)
                     /locus_tag="VWZ30_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9335770.1"
                     /translation="ETSSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTEIP
                     WVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIPATH
                     NEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPISNH
                     EEMKRIISKVDLLFQKVSQFFE"
BASE COUNT         1153 a          466 c          558 g         1082 t
ORIGIN      
        1 tttctacatt ggttgaaatt gtcagaggtg gctctccacg accaatcaaa gattatctta
       61 cttctgaagt agatggaata aattggataa aaataggtga tactgaaaag ggtgaaaagt
      121 atataaataa tgttaaagaa aaaatcaaaa aatcagggct taacaaaact agatttgtaa
      181 aaaaaggtac atttttgtta actaattcta tgagttttgg tagaccttat attttgaatg
      241 ttgatggtgc aatacacgat ggatggttgg ctatttcgaa ctatgaaaac tcattaaata
      301 aagattacct attctatatt ctttcatcaa atgtagttta ttctcaattt ctatctctaa
      361 ttagtggagc tgttgtgaaa aacttgaata gtgataaagt tgcttctatt cttatccctc
      421 tccccccact atccgaacaa caacgcataa tagaagcaat cgaatcagct ttagaaaaag
      481 tagatgaata tgctgaaagt tataatagac tagaacagct agataaagaa tttccagata
      541 aactaaaaaa atctattctt caatatgcta tgcaaggaaa attagttgaa caagacccaa
      601 atgatgaatc agtcgaagtt ttacttgaaa aaatacgagc agaaaaacaa aaactctttg
      661 aagaaggcaa gattaaaaag aaagatttgg acatttctat tgtttcccaa ggagatgata
      721 gctcttatta tgaggaagta ccttgtgaaa taccagaaag ttgggaatgg gtgaggttaa
      781 acgatataac ctcctacatt caaagaggga agtctccaaa atattcaaat attcctatat
      841 accctgtgat tgctcagaaa tgtaatcaat ggtctggctt ttctattgac ttagcaagat
      901 ttattgatcc cgaaacagtt catagttacc agaaagaaag attactaaga gatggtgatt
      961 taatgtggaa ttcaacaggt ctaggtacgt taggacgatt agctatttac catgaaaata
     1021 aaaatcctta tggctgggct gttgcagata gccatgttac agttataaga gtcttatcgg
     1081 gagttattaa ttgccatttc atctataatt ttctttcctc tcctattgtt cagtcagtta
     1141 ttgaagaaaa agcgtcgggt agtacaaaac aaaaagaatt attaacgaaa actataaaag
     1201 aatatttaat ccccctccca ccccttcctg aacaatccag aatcgttgat aaaatcgaac
     1261 aatttttcgc ccatattgac gcactaattt agtataatag ttttacgtgc ggtggaattt
     1321 ctatcaaaca taagatagga gttttacgtt gaaagatttt gaattacatt tgaagaaagc
     1381 tggtttagct gaaaatactg tgcgttcgta cctttatggt gttcgatttt tcttggagaa
     1441 ttacgaactc aaaatggaag atttatttga atacaaagga tatttattag acaattttaa
     1501 acctaaaact gtcaatttgc gactacaagg agtaaataaa tatctgactt ttattggtca
     1561 tgatgatttg aaattaaaat ttgtcaaagt acagcaaaaa ccgtttttag aagatgtcat
     1621 tagccacgct gattaccttt ttcttaaacg tagtttgaaa aaagatggta ttttaaaatg
     1681 gcattttgtc gtttggtttt taggtgcaac tggggctcgt gtaagtgaac ttattaaact
     1741 aaaagtagag catgtagaaa tcggttattt tgacatttac tcaaaaggtg gtaaaattcg
     1801 tagactgtac attcctaaaa aattaagaaa tagttgtctt agttggttag aatcagaaaa
     1861 tcgccgaagt ggttatttat ttttgaataa atttaacgaa ccaattacag caagaggagt
     1921 tgctcaacag ttaaaaaatt atgctgataa atacaaaatg aatcctaaag taatttaccc
     1981 tcattctttt aggcatttat ttgctaagaa ttttttagcg aagtataatg atattgcctt
     2041 gcttgcagat ttgatgggac acgaaagtat agaaactact cgaatttatc taaggaaaac
     2101 agctactgaa caacaaaata ttgtagataa aattgttaat tggtaaaaaa taacaggtgg
     2161 tcaaactgac tacctgctat ttttgtgatt atggctctta ttatgggaat atacctatga
     2221 attgggttgt tataaaaata aaagatattt tttcaataaa tacaggtctt tcttacaaga
     2281 agggcgattt aagcattaat aataaaggta ttagaattat acgtggtggt aatattaagc
     2341 ctttagaatt ttctctgttg gataatgatt actacattga tacacaattc atctcctctg
     2401 agcaagttta tttaaaacat aatcagctaa taacacctgt atcaacctct ttagaacata
     2461 ttggaaagtt tgcaagaatc gataaagact atgatggtgt tgtggctggt ggattcattt
     2521 tccaattaac accattcgaa agttcagaga ttatttcaaa atttctatta tttaacttgt
     2581 cctctccgtt attttataaa caattgaaag caataactaa actatcaggt caagctttat
     2641 ataatattcc taaaactaca ctaagcgagc tattaattcc gttagctcct tttgaggaac
     2701 aggaacttat tactcaaaaa attgagaaac tttttgaaaa agtaaatcaa ctttggaaat
     2761 gattcttttc atctcttcat gattagaaat agggattaat aattcggaga tactggtact
     2821 atttaatgtt ttccctttga tagcatcttt tgaatcacct aaagtagaga taagtggcaa
     2881 aaatatcatt aagtaatctc tgataatatt ttctttatta gcatagggga atatcgatat
     2941 aatggcttca ttatgagtgg caggaatatc caatatggca acttttccaa tagataattt
     3001 aaagctcatt aataaagttc ctttaggtga aatgtctatt ttctttgatt ttaatgctaa
     3061 tttagaaata gattctctcg tattagttac ataaccagat ataggcatat ctgatataga
     3121 tacccagggt atttcagttc cccaaaaagt agcttcactg cgtggaggag tttttcctat
     3181 tctgaagtta actaggctag caaatttaat atatctccat gcttctggga tttcatatat
     3241 aggataagag gatgtttcg
//
LOCUS       DAVKDZ010000137         3218 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000137 DAVKDZ010000000
VERSION     DAVKDZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.139"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(184..795)
                     /gene="rpsD"
                     /locus_tag="VWZ30_002127"
     CDS             complement(184..795)
                     /gene="rpsD"
                     /locus_tag="VWZ30_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU9335771.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(990..2042)
                     /locus_tag="VWZ30_002128"
     CDS             complement(990..2042)
                     /locus_tag="VWZ30_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9335772.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2039..2737)
                     /locus_tag="VWZ30_002129"
     CDS             complement(2039..2737)
                     /locus_tag="VWZ30_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9335773.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2904..>3218)
                     /locus_tag="VWZ30_002130"
     CDS             complement(2904..>3218)
                     /locus_tag="VWZ30_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9335774.1"
                     /translation="INVKETSDTANDQMKKSGMKPETPAPGTTNSMPADTMASSTNTM
                     AGENMAASANKMSDTMMSDDKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLKNKKE
                     EN"
BASE COUNT          892 a          723 c          614 g          989 t
ORIGIN      
        1 aagattatac cacattgtgt actatttttg gttcatttta ctatatcact ttggactata
       61 tcggtctagt tatgtctaaa cgattatagc tggatactaa gagaaatcaa aaaaagcact
      121 ctaggataga ggcctaaagt gcttagtttc aaggctttac agcctatcat atttaataaa
      181 atattacaac attttgttgt agaattcaac gacaagtgct tcgttgattt ctgggttgat
      241 ttcgtcgcgt tctggcaagc gagtcaatga accttccaat ttttcagcgt cgaatgatac
      301 gaatgctgga cgtccaagag tagcttctac tgcttcaagg attgctggaa ctttcaatga
      361 tttttcacga actgagatca cttgacctgg agttacgcgg tatgatggga tatcaacgcg
      421 tttcccgtca acaaggatgt gaccgtggtt tacgaattga cgagcttgac gacgagtagt
      481 cgcgagacca agacggtaaa caacgttatc caaacgacgt tccaaaagaa gcataaagtt
      541 gaaacctagg attccgcctt tgatttttgt agcttgtacg aacaagttac ggaattgttt
      601 ttcacctaca ccgtaagtga aacgaagttt ttgtttttca gccaattgca aaccgtattc
      661 tgacaattta gaacggttgt ttggtccgtg ttgtcctggt acgtagttac gacgtgccaa
      721 ttctttacct gtacctgtaa gtgaaaggcc aaggcgacga gcttgtttcc aagatggtcc
      781 tgtataacgt gacatatgaa tgtcctcctg atataaataa tatttcggtg gaaatagtca
      841 cttagaaagc cctgattcgt gcagatgccc ttcgcctaaa cagccaaggt tacttgtcat
      901 aagacacctg ttgacgagct tcatgctttc ctgctgatat ttcacacaaa atctattcta
      961 ccatgaatag ctggatttgt aaaggggttt taggctttat tttcactacc agagaggttg
     1021 agaacgaggg taaaggtact tcctagaccg tactggctgc tgactgtgat ttccccaccc
     1081 aattgatggg ccaattcacg cgcaatcgca agtcctaatc catgaccacc tgtcttcatg
     1141 ttacgcgaag tttcgacacg ataaaggcgt ttgaaaatat tttccaaatc ctctggggca
     1201 ataccctgcc cttcatcggt cacactgatt gaaagctggt ccttctccag cttagccacc
     1261 acttccagct tggttcctgg agcagaatat ttaaaagcgt tatcgaccag attcaccaag
     1321 atacgagaaa gcttagcata atctccctca atccgggcag actctgggat tacctgcaag
     1381 tggacatctc ttctctcctg ctcaatcaaa aactgaaatt cactcatgca ctcaattaag
     1441 agcttgtcca gaaaaatact gtctttactg gtagtttcca cctgatttct agctgtgttt
     1501 agggtcaaaa aattcaactc ctcaaccagt ttattgagcc tctccgtctg gcgtccaatg
     1561 gttgctagat aatgagcttg ctccgactcc ttgataatcc catccaaaat cccttctacc
     1621 gtcgcttgga tcgaagtgat aggagtctta atatcatgcg acaactgagc aatcatcaag
     1681 cccttttctc gttcgctttc ttccaaggaa tcaaagctta cctgcaaatc atgggacatc
     1741 tcattaaaag tttgccctaa ttgctgaaat tctacaggac cttgaacctc caaatttgaa
     1801 ggaaaatcct tggccgctac ccgcttggca tgctccttga gtttgcccaa cgacgtaaag
     1861 actggcaata ggagaaagag actaatccca gcaccgacaa ggctggcaac gatggtcatc
     1921 ccaagcaaaa agtaaatctc gcctttcgca atcagcattt tttgaacagc ccaaaaaaca
     1981 accaaaatgg ttaagagggt tgaaataata tatccaacca aaatataact ttttagtttc
     2041 atgtttgtcc tctcggtttc tctatcttat atcccaaccc ccaaactgtc ttaatagtgg
     2101 gcgtttggtc actactatat tttgccagtt cctgtcgaag agcatggata tgcacattca
     2161 aggtattggt gtcatccacg tagtcttctt tccagatctt ttcatagagg tctgtcttgg
     2221 agaaaactcg ctctggattg ctagctaaaa tccacagcaa ttcaaatgat ttaacagtta
     2281 aatccagcat ttcttctcct atttgaactt catgactact atgattcatt tttagattgc
     2341 caagggaaat cagctctgtt tcgcccccac gatgaaggcg gcgcaaaata ttgtggacac
     2401 gcaaaaccag ctcacgtggg ctaaaaggct tagcaataaa atcatctgct cccaagctca
     2461 ggccgtaaat cttgtcctgt tcactggtct tagcagtaat aaataggaaa ggctgctctg
     2521 gtgataagta ttgaacctca ctgattaaat cataaccatc catccgaggc atcatgacat
     2581 ctgtgataat caaatcaatc ggttttttct tgaacagctc tagcgcttcc agtccatcat
     2641 gggctaccaa gacctgataa cctgcctgaa ttaagtatct ctgatgaata tctgtgattt
     2701 ctacctcgtc atcaacgagt aaaattgtct ttcccatctg tctctccttt gataaaaaca
     2761 gtgctatact gctttaagta taacactatt tttattgttt tacgatgatt ttaatctgct
     2821 attgaaagaa agtcctgatt gctgattgag ttggtaaact agccttagtc agcccactat
     2881 ccatcattta tttccttagc tgattagttt tcttcttttt tgtttttcaa acctagacca
     2941 ccgagtaaac ctgcaagcgc aagcccaagg aaaccaatac ttgccattga tgtttgagtt
     3001 tcaccagtat ttggtagcat agctttatca tctgacatca tcgtatcaga catcttgtta
     3061 gcagaagcag ccatgttttc acctgccatc gtgttggtag aacttgccat ggtatcagca
     3121 ggcatgctat ttgtagtacc tggagctgga gtttcaggct tcatacctga ttttttcatc
     3181 tgatcatttg ctgtgtctga tgtttcttta acatttat
//
LOCUS       DAVKDZ010000138         3160 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000138 DAVKDZ010000000
VERSION     DAVKDZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.140"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            308..1594
                     /locus_tag="VWZ30_002131"
     CDS             308..1594
                     /locus_tag="VWZ30_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9335775.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDNIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAVQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQAGDMVRALKEGLINFYKEDGTSVTVRA"
     gene            1609..1920
                     /locus_tag="VWZ30_002132"
     CDS             1609..1920
                     /locus_tag="VWZ30_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335776.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCLFCFSRFNPGCQNHKERYF
                     K"
     gene            2180..2908
                     /locus_tag="VWZ30_002133"
                     /pseudo
     CDS             2180..2908
                     /locus_tag="VWZ30_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
BASE COUNT          980 a          547 c          683 g          950 t
ORIGIN      
        1 taaaaaagcg atattctaga ctgtatcaat ccttttccat tataggagca acagagtggg
       61 agtagtcatc taaggactaa tttatgtatt tttacgagtc agtatctttg gatactggtt
      121 tttacttttc tagacttttt gactacttgt taaaactggg ataattttcg actgtttaac
      181 agttattatg caaagtctaa aagattagaa ttgtcaaaac aatccgtcta ggcttgattt
      241 tatcctttat ttactataaa atgagaagga aaaatgtcaa acttttatat tgcaaatagg
      301 agaaatcatg acaaaaacat taaaacgtcc tgaggtttta tcacctgcag ggactttaga
      361 gaagctaaag gtagctgttc agtatggagc agatgctgtc tttatcggtg gtcaggccta
      421 tggtcttcgt agccgtgcgg gaaactttac tttcgaacag atggaagaag gcgtgcagtt
      481 tgcggccaag tatggtgcca aggtctatgt agcggctaat atggttatgc acgaaggaaa
      541 tgaagctggt gctggtgagt ggttccgtaa actgcgtgat atcgggattg cagcagttat
      601 cgtatctgac ccagccttga ttatgattgc agcgactgaa gcaccaggcc ttgaaatcca
      661 cctttctacc caagccagtg ccactaacta tgaaaccctt gagttctgga aagagctagg
      721 cttgactcgt gtcgttttag cgcgtgaggt ttcaatggaa gaattagctg agatccgcaa
      781 acgtacagat gttgaaattg aagcctttgt ccatggagct atgtgtattt catactctgg
      841 acgttgtact ctttcaaacc acatgagtat gcgtgatgcc aaccgtggtg gatgttctca
      901 gtcatgccgt tggaaatacg acctttacga tatgccattt gggaaagaac gtaagagttt
      961 gcagggtgag attccagaag aattttcaat gtcagccgtt gacatgtcta tgattgacaa
     1021 cattccagat atgattgaaa atggtgtgga cagtctaaaa atcgaaggac gtatgaagtc
     1081 tattcactac gtatcaacag taaccaactg ctacaaggcg gctgtggatg cctatcttga
     1141 aagtcctgaa aagtttgaag ctatcaaaca agacttggtg gacgagatgt ggaaggttgc
     1201 ccaacgtgaa ttggcaacag gtttctacta cggtatacca tctgaaaatg agcagttgtt
     1261 tggtgctcgc cgtaaaattc ctgagtacaa gtttgtcgct gaagtggttt cctatgatga
     1321 tgcggtacaa acagcaacca ttcgtcaacg aaacgtcatt aacgaagggg accaagttga
     1381 gttttatggt ccaggtttcc gtcattttga aacctatatt gaagatttgc atgatgccaa
     1441 aggcaataaa atcgaccgcg ctccaaatcc aatggaacta ttgactatta aagtcccaca
     1501 acccgttcaa gctggagaca tggttcgtgc attaaaagaa ggactcatca atttttataa
     1561 ggaagatgga accagcgtca cagttcgagc ttaagaaagg aaaaggaaat gatagaggca
     1621 cagggtttct tagtggataa gcaaacaaga tgcattcatt accatagcaa gctggatatt
     1681 attgctttac aatgctatga ttgtaaaaag tattatgctt gttatcggtg tcatgattca
     1741 ttagaaaatc acccttttga gccttatccc ttatctttga tacaggataa gcctatttta
     1801 tgtggtgttt gtctaaaact attaacatat aagcaatata aagaaagctt aagttgcctc
     1861 ttttgttttt ctcgctttaa tccaggttgc caaaatcata aggaacgcta ttttaaatag
     1921 cagatcatct agttttgaag taggagaaaa ctcaattcca agagaaaatg aagtaaatct
     1981 tcccacaata aaacgcataa tatcaagatt gttcaatacc tgatactatg cgtttttaag
     2041 attttaaaga cttttttcct agcctctttt tactttatct ggtattttga ctacttgtta
     2101 aaactgggat aattttcgac tgtttaacag ttattatgca aagtctaaaa ggttagaatt
     2161 gtcaaaacaa tccgtctaga gtatgcgtga tgccaaccgt ggtggatgtt ctcagtcatg
     2221 ccgttggaag tacgaccttt acgatatgcc atttgggaaa gaacgtaaga gtttgcaggg
     2281 tgagattcca gaagaatttt caatgtcagc cgttgacatg tctatgattg accacattcc
     2341 agatatgatt gaaaatggtg tggacagtct aaaaatcgaa ggacgtatgg agtctattca
     2401 ctatgtatta acagtaacca actgctacaa ggcggctgtg gatgcctatc ttgaaagtcc
     2461 tgaaaagttt gaagctatca aacaagactt ggtggacgag atgtggaagt ttgcccaacg
     2521 tgaactggct acaggatttt actatggtac accatctgaa aatgagcagt tgtttggtgc
     2581 tcgtcgtaaa attcctgagt acaagtttgt cgctgaagtg gtttcttatg atgatgcggc
     2641 acaaacagca actattcgtc aacgaaatgt cattaacgaa ggggaccaag ttgagtttta
     2701 tggtccaggt ttccgtcatt ttgaaaccta tattgaagat ttgcatgatg ccaaaggcaa
     2761 taaaatcgac cgcgctccaa atccaatgga actattgact attaaagtcc cacaacctgt
     2821 tcaatcagga gacatggttc gagctcttaa agaggggctt atcaatcttt ataaggaaga
     2881 tggaaccagc gtcacagttc gtgcttaatg tagttgttta gtgttaaaaa attatgcaaa
     2941 gctccatata caacacttaa acgagattaa agaatggcga aatcccttga tgcgcaagag
     3001 attagctgtc cttttttatt ttttaagtga taaagtcgga gcttaggtat caaagcctat
     3061 caaattaaac aaagaagcga tgtcttagat attttgaaaa aaattaataa gcagaaaact
     3121 ctctattatt ttgttgtaga gagtttttta ttaataaaat
//
LOCUS       DAVKDZ010000139         3102 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000139 DAVKDZ010000000
VERSION     DAVKDZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.141"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..490
                     /locus_tag="VWZ30_002134"
     CDS             <1..490
                     /locus_tag="VWZ30_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9335777.1"
                     /translation="IKRYWKLIQQDSRKLSDKRFYRPTFRMHLTNKEILNKLLSYSED
                     LKHHYQLYQLLLFHFQNKEPEKFFGLIEDNLKQVHPIFQTVFKTFLKDKEKIINALQL
                     HYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRISIALNIKKERTKFVLSRAYLTSTH
                     YS"
     gene            complement(540..1232)
                     /locus_tag="VWZ30_002135"
     CDS             complement(540..1232)
                     /locus_tag="VWZ30_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEU9335778.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            complement(1391..>3102)
                     /locus_tag="VWZ30_002136"
     CDS             complement(1391..>3102)
                     /locus_tag="VWZ30_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEU9335779.1"
                     /translation="YRKVFVPDKTNNEWFKEQTKAYIVEEKSTIKEVNDKQSKAGTPQ
                     SIGVYDRLTSPSWKYPSMVLPLLTLPEKSVFIIANISTIGFGAYDRYRSKEHPAGTNL
                     NDYVEAKAREAAVRFRDHYDYWYKILDNNNKEKLYRSVLVYDAFRFGADDKGERETKQ
                     ANFETDHPAIKHFFGPAGNNVVHNSNGAYATGDAFYYMAYRMLDKDGAVTYTHEMTHN
                     SDREIYLGGYGRRNGLGPEFYAKGLLQAPDHPDDPTITINSILKYEESEDPTRLQVKD
                     PTKRFNNAEDLQKYMYNMFDVIYMLEYLEGNAVVKLDISKKNELLRKIENKFETDPDG
                     SNVYATNVVRYLKPEELTKLTSFNSLIENDVITRRGYENGNDNTFKRNGYYTIKLFSP
                     IYSALSNNEGTPGDLMGRRMAFELLAAKGFKDGMVPYISNQYAEEAKAKGKVIKSYGK
                     EVGNVTDELVLQKIFNNRYSSWVEFKKAMYNERIAKFKKLMSISFDNPNGNWFRKDRV
                     TIKNIEDLQRMITTAVNEDAEDYLVNIYPERSRVLKLKKAIFKAYLDQTNDFRSSIFD
                     EEK"
BASE COUNT          928 a          625 c          447 g         1102 t
ORIGIN      
        1 tatcaagcgc tactggaaac tcattcaaca ggatagccgt aaactgagtg ataagcgatt
       61 ttatcgtcct acttttcgta tgcacttaac caataaagag attttaaaca agcttttgag
      121 ttattcggaa gacttgaaac accactatca gctctatcag ctcttgcttt ttcacttcca
      181 gaataaggaa ccagagaaat ttttcggact cattgaggac aatcttaagc aggttcatcc
      241 tatttttcag actgtcttta aaaccttcct caaagataaa gaaaagatta tcaacgccct
      301 tcaactacac tattctaatg ccaaactgga agcgaccaat aatctcatca aacttatcaa
      361 gcgcaatgcc tttggttttc gaaactttga aaacttcaaa aaacggattt ctatcgcttt
      421 gaacatcaaa aaagaaagga cgaaatttgt cctttctcga gcttatctga cttcaaccca
      481 ctacagttga caaagagcca cataaaaagc ctagaaatca attctagact tacaattttt
      541 tattttccaa ggtagtattc agaaactacg ttcaattttt cgtcgaattc gaataccaat
      601 ggtgggaagt tagggatttc cacgtccatg atctcgtcat ctgacaaacc tttgatgtgt
      661 tttacaagag cacggattga gttaccgtga gctcctacga atacgttttt accatcttta
      721 agagctggag cgattttatc ttcccagaat ggaagggcac gttccaaagt cactttcaag
      781 ttttcagcat ctgggataac tgagtcgtca agtgaagcgt aacgacgatc agtgtgagct
      841 gagtgctcat catcacggtc catgtttgga ggcaatacat catatgaacg acgccagatg
      901 tgaacttgct catcaccaaa ttgctcagca gcttcagctt tgtttttacc agtcaaacca
      961 ccgtagtgac gttcgttcaa acgccatgat ttttcaactg gaacccacaa ttggtcagaa
     1021 gcttcaagag ccaagtttgt tgttttaatc gcacgtttca atactgaggt gtaagcttgg
     1081 tcaaattcga taccagcttc tttgatcaat ttaccagcgt caatcgcttg ttgtgtacct
     1141 ttttcagaca aatcaacatc agcccaacca gtgaaaaggt tagctttatt ccattcagac
     1201 tcaccgtggc gagcaaaaac caattttacc attagatgga ttctccttta aattttccga
     1261 ggtttcccct cgtttatcta ttctatttta cacaattttt cacaaaaaag ctagtcaaaa
     1321 ccaaaaagga aagagtttca cacatgaaga aacgaaacct atcttcatgt gctaccccta
     1381 attagtaaat ttatttttcc tcatcaaaaa ttgaacttct aaaatcattt gtttgatcaa
     1441 gataagcttt aaagattgct tttttcagtt taagaactct acttctttct ggatatatgt
     1501 tcacaagata atcctcagca tcttcattga cagcagtagt tatcattctt tgaagatctt
     1561 ctatattttt tattgtcact ctatcttttc tgaaccaatt tccatttggg ttatcgaagc
     1621 taatgctcat taatttttta aatttagcta ttctttcatt atacattgct tttttaaatt
     1681 ctacccaaga tgagtaacga ttattaaata ttttttgaag tactaactca tcagttacat
     1741 taccaacttc cttaccatat gacttaatca ctttaccttt agctttcgct tcttctgcat
     1801 attgattaga aatatatggt accattccat ctttaaatcc ttttgcagcc aacagttcaa
     1861 atgccatacg acgacccatt aaatctcccg gagttccttc attattactt aaagctgaat
     1921 atattggtga aaatagttta attgtataat atccatttcg tttaaaagta ttgtcatttc
     1981 cattttcgta tcctcttcta gtaataacat cattttcaat taaactatta aatgaagtta
     2041 atttagtaag ctcttcaggt tttaaataac gaacaacatt agttgcatag acattacttc
     2101 catctggatc tgtttcaaat ttattttcta tttttctcaa caactcattt ttcttagaaa
     2161 tatctaattt aacaactgca ttaccttcta gatattctag catataaatt acatcaaaca
     2221 tattatacat atatttttgc aagtcttctg cattattaaa tcgttttgta ggatctttta
     2281 cctgaagtct agttggatct tctgattctt catatttcaa tattgagtta atcgtaatag
     2341 taggatcatc aggatgatca ggagcttgta acaatccttt tgcataaaac tctggtccaa
     2401 gtccatttcg tcttccatag cctccaagat aaatttctct atctgagtta tgtgtcattt
     2461 catgtgtata agttaccgca ccatctttat caagcatacg ataagccata tagtagaatg
     2521 cgtcacctgt agcatatgct ccattagaat tatgaacaac attatttcct gctggaccga
     2581 agaagtgttt tattgctgga tgatcagttt caaaattagc ttgttttgtt tctctttctc
     2641 ccttatcatc tgcaccgaat ctaaatgcat catagactag gacacttcta tataattttt
     2701 ctttattatt attatctaga attttatacc agtaatcata gtgatctcta aatctaacag
     2761 ccgcttctct agcttttgcc tcaacatagt catttaaatt agttcctgct ggatgttctt
     2821 tacttctata tctatcataa gcgccaaatc ctattgttga aatgttcgct atgataaaca
     2881 ccgatttttc aggtaatgtt aatagcggca ataccatact tgggtatttc caagatgggc
     2941 tggttaatct atcatacacc cctatagatt gcggagtacc tgcttttgat tgtttatcat
     3001 ttacctcttt aattgtagat ttttcttcca ctatataagc tttagtttgt tctttaaacc
     3061 attcgttatt tgttttatct ggcacaaaaa cttttctata tt
//
LOCUS       DAVKDZ010000140         3087 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000140 DAVKDZ010000000
VERSION     DAVKDZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.142"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(186..482)
                     /locus_tag="VWZ30_002137"
     CDS             complement(186..482)
                     /locus_tag="VWZ30_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HEU9335780.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
     gene            complement(1025..1918)
                     /locus_tag="VWZ30_002138"
     CDS             complement(1025..1918)
                     /locus_tag="VWZ30_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9335781.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKDSLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNVAEAESFYEKAISLAEL
                     LDDQVLVQKILAEKQIDFPTT"
     gene            complement(2672..2902)
                     /gene="blpU"
                     /locus_tag="VWZ30_002139"
     CDS             complement(2672..2902)
                     /gene="blpU"
                     /locus_tag="VWZ30_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEU9335782.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
BASE COUNT         1045 a          626 c          334 g         1082 t
ORIGIN      
        1 taacgattca aagtagcgta atctactacc aaaaaaagta aaaataattt cccttgaaaa
       61 ggaagatagc ttttaaaata gattgacaaa cttatgataa caaaaaacaa aagggttatc
      121 ccaataaaat taagataacc taaaatactt tcacattctt ttttagaaag tattttcatc
      181 tagttctacc atagagctgc tttagcagca cctttctttt tcaatatata aagaactgtt
      241 gctgcaatac ccgaaccaat agaacctact cctgtgattg cacctacaat tgaaattact
      301 gctgtaactg tactataagc actaatcaaa ttgactacag tagcagcagc ttttgtcgaa
      361 attcctaatg tcccagccaa cataggaaaa gaaacaacac ctaagcttaa ccaactaatg
      421 actgctaaca ttgctgaaac aataagaaat ttattatgaa attccattct tttagatttc
      481 attttagtgt actccttttg tatattatgt atgttttatt gagagttaat tcgcacatgt
      541 tgtattccta actctcttca aatgttcgtc tatattttat actactttta ttaacatatc
      601 tataataaat aaattactac tacattttcc tagattatca aattactttt atatcactaa
      661 gactcaataa aagataatgt ctatttaact tttatacgat tactacctcc tttttagttt
      721 acacttccta catcataaat aaacatactc aaccctcaca atctccaaat aactcttcta
      781 tatcattgaa aattgagttt catcctctat actaatgtag gaaacctatt aaaacattcc
      841 tttaatccaa tataggaata ccttatgcca ttaatctcta cctgatatta tagatagaac
      901 cagactccta tacttaaaag gacaaacaac gttccaacca taataaactt cctttctttt
      961 tggaatcgtt ttctttatga gtttagtata acaaaaacat gaaaaaaaca acatgactta
     1021 gaagtcaggt tgttgggaaa tctatttgtt tttctgctaa aattttctga actaatacct
     1081 gatcatctag caattctgct agagaaatcg ccttttcata gaaactttct gcctctgcca
     1141 cattcttatc tacaaataaa gcatatttcg cctttaacaa aaatagatga ggtcggtaaa
     1201 aaacatgtct tgttttagca ataattttct caaatgtttg aatatatatc agactatctt
     1261 ctgtctgaat ttggataaat aggctcagaa gaactttttc catctgaact aggctatcct
     1321 tatcttcata ttctgaacat agcaatactt tttgagctaa ctcctcaaaa tgttctttat
     1381 cttctaatcc catagcacaa caaaagaaat acaattcaat aatcaacaaa tcatttacag
     1441 aaaaatattt cttttttaat aactgctgga aatattcttc cactaaccct aaaccgtagg
     1501 tcacatctga actaccataa atatcaaaac gagcttgtgt cacatccaca attaactgct
     1561 cttcctcagg caaattctca taatagtttt catatatttc ctcaagtata gcttcttttc
     1621 gttcgattct tcccttatca gcgtaggttt gagaatgaat cagctcatct ttgagtttta
     1681 ggaatccttt tggaatctct ataaccttaa catccacaat attctgtaat gatactccaa
     1741 gtttatccgc aatcaataat aatttaggaa gtgttgctaa agcttgccca ttttcaatac
     1801 gtgctagctg acgaatcgtc aactctgatt catcaccaca aatatctatc tgagtttttc
     1861 cacaagatcg cctaatagtt ttgattttat aaccaatttc acgtttcaac tcatccatag
     1921 taatactcct taagttgatt acaaagattt ctttttcttc ctaattcttt tgtcaatcat
     1981 atataaaacg ataagcgaaa acagcggtag aacaatataa ctcttttcac tcattatcag
     2041 ctgaaatccc agggttgcta gtatcttaaa gatgaaaata ttcatcaaaa atagaaaaac
     2101 aaatactaaa aaatatttta aaaccttctt catttctttt cttcaacttt cctaatttct
     2161 acacctattt tatcgaaaat atatataaaa ctccaaataa acacttcaac tgcacttttt
     2221 ttggcaaaaa atagacagaa caaacatcct acctactaaa aatatgatat gaaaggaaaa
     2281 tgatttttta ctgacaccct aggcaagctt gggacaaata aatcattgat agcaacattt
     2341 acgacatcct atttaaagtt ccttcaattt ctaatgtccc cgctatttct aagctacctc
     2401 tggtatgatc tattcctagt cagaatagat aacaccgttc taaattacga ttcatagtta
     2461 ctcctttttt gactcgttac tataagactc ctctttacat gcgcaaaata attttgattc
     2521 agattcatca tttatagttt tgattatttt ttaacagatt tttctgtttt cttctccgaa
     2581 gactttaaaa atttttttct aattaaatca tccatataag gaccaaatat accaactact
     2641 aaaccaataa taaaactttt aaaatccata attaccacca acatgtcgct gcataggcca
     2701 cacctccaag tatagctcct cccgcagcac cagttgctgc accttgccat gttcttgttt
     2761 taattcctag ctgaagacct cgtgctgctc ctcctccaac acctgctttg gcaaaatctc
     2821 cccaattgca tccgccacct tcaacgcaag caagcatctc agtatccata atttcaaatt
     2881 gtgacatctt ttttgtattc ataacgaata cctctttttt atttttaata tttgtcttgt
     2941 tacaaacttg acaagtttag tataacagta tctattaatt tttttcatcc aaatcttgaa
     3001 ttggcatcga aacgtcttga attagctttt ttgtttcaaa atcatctcta tttttaaaaa
     3061 agatgttttc taatcacttt tttacta
//
LOCUS       DAVKDZ010000141         3076 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000141 DAVKDZ010000000
VERSION     DAVKDZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.143"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..165
                     /locus_tag="VWZ30_002140"
     CDS             <1..165
                     /locus_tag="VWZ30_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9335783.1"
                     /translation="VSFAISSKGEKMKVENISYRVDHRILFDNISFDTSSSDVTLITG
                     KNGTGKSTLL"
     gene            complement(531..1841)
                     /locus_tag="VWZ30_002141"
     CDS             complement(531..1841)
                     /locus_tag="VWZ30_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU9335784.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTSIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2077..2949
                     /locus_tag="VWZ30_002142"
     CDS             2077..2949
                     /locus_tag="VWZ30_002142"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU9335785.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          942 a          516 c          703 g          915 t
ORIGIN      
        1 gtttcttttg ctattagttc aaaaggagaa aaaatgaaag tagaaaatat ttcgtatagg
       61 gtggatcatc gtatattgtt tgataatatt tcttttgata cttcgagttc agacgtgaca
      121 ttaattactg gtaaaaatgg tacaggaaag tcaactttac tatagtagat tgaaactaga
      181 atagtacaca tctacttata aaatattgtt agaaatcgat ttgactatcc tgatctattt
      241 gtcctgttct tatttcattt cactacatct caaattgagt atgacgaagt gcgctcccat
      301 gtcctgggaa cgcactttct tcatattttt catattcttg aatccatcga taaagactat
      361 tgggatgaat ttttaaagtt gaactaatta tttttacagg atgaggttta cagcagagag
      421 tttgaaggct ttatcaaagg tttttcttgg cataatgact tttcctcgtt tccacttaat
      481 tttgtgtcta ctttattata ccaagtccac ccttaagtta gataatgaat ctaacttaag
      541 gaagctagaa ggatgagaat ccaggtggtc aagagtccca aacttaagct gatggggaca
      601 cccagaataa tttgcttttt gaaggcaagg ccgcgttcct ctatattggg aagtgagagt
      661 tgaatgagag aaccagctga tgaaaagggt gagatattag tagatagagc gccaataacg
      721 gtggctgttg tgagtaagtg aatatcaatc tgaggatttt gagcactgat gatagcaatg
      781 atgggaaaga gggctggagc tacaacggat agggtggaac taaagagtga catcaccccg
      841 gctatcacac aaaagaacag aggtaaccag aaatgaggaa tggatgttgt catgaggtgc
      901 cctatcagtg tgactaaacc tgacttgacc gctagagaca ttagtaagct catgccgcag
      961 agcatgataa ttgtagccca gggaacttta gccaaaatgg cttcttgctt ccctaatttg
     1021 agccttaagg cgaggcagac catgagtatt gagacaaagc caatatcaaa tgtttttcga
     1081 taagtagcta tccaggcgat gtttgggaaa atgagatgca acaagggaaa aagccaaacc
     1141 aaaaccatgc tgctgatcat gagcaaggtg gtttgtcttt gaaccttgct gaggagtggt
     1201 ggttggtcaa tagtcaagga tgagtttgtt cttcccttac tatagtgact gtaacaggat
     1261 aataaaagca agacgatgag tgggtagata atgctgacga taaagatatg attgccaagt
     1321 gaaaaagctt gctcttccca tcccatttgc ttaaacaggc cttgaaagac aatgcctgag
     1381 ctactggtta tcaaattagc ccctcctgaa gctccccaat tgacggcttg agctccaatc
     1441 aaagggtgtt tgtccgcttt ttgacagagg gtaatcgcta gaggacagca aacggccata
     1501 gtagtgaaaa atccagcacc taaagcagac aaaagggttg ccatcaggta taaaatcatg
     1561 tagagggcgt tagggtgggt gcgtgtgcgg tagagaatgt gttgagccaa aacatcaaga
     1621 gtaccgttag ttgttgcaac gttataaaag agagagacgc taaaaatggt aaaaaagagt
     1681 gaggttggcc aaaaatgaag aagttctttg gggcttaatc ccatgagagt ggttgcgatg
     1741 aggtaagaaa aagcaatagc cagcaggcca atattgattt tggtgcggta accaattcca
     1801 atggctagag caatggcgct aatcattatt aaatgaatca ttgaattgtc ctttcagtta
     1861 gaatatggaa ataggttggc aattttggcg ctcaaaagac cacctgtttc ctattggtac
     1921 ttgactatta tacacaaaaa aatacaattg tcttgacaat tgtattgaca cttgttagaa
     1981 ttaaaaatga ggtattcagg cttgtgattt cctatggaag ttaatagtaa ttgcctctag
     2041 tgcttacaag tgatattaaa aatagaggac ttagtgatgt caatcatttc aactgattta
     2101 accccttttc aaatagatga tacattgaaa gcagccttgc gagaagatgt tcattccgaa
     2161 gattacagta ccaatgccat ttttgatcat catggccaag ccaaggtgtc gctttttgcc
     2221 aaggaagctg gtgttttagc gggactaacc gtttttcaaa gggtttttac cctatttgat
     2281 gccgaggtga ccttccagaa tcctcatcaa tttaaggatg gggatcgttt gactagtggc
     2341 gatttggttt tagaaatcat aggctcggtg agaagtctct taacatgtga acgcgttgcc
     2401 ttgaattttt tacaacattt atcagggatt gcttcgatga cagctgctta tgtagaagcc
     2461 ttaggcgatg attgcattaa ggtatttgat actcgaaaaa ctactcctaa tttacgtctt
     2521 tttgagaaat atgccgtgag agttggcggt ggctataatc atcgctttaa tttatcagat
     2581 gctatcatgc taaaagacaa tcacattgcg gcagtaggta gtgttcaaaa ggcaattgct
     2641 caagcgcgtg cctatgctcc ttttgtgaaa atggtcgagg tggaagtgga aagccttgct
     2701 gctgccgaag aagctgcggc ggcgggtgct gatattatca tgttggataa tatgtcattg
     2761 gaacagattg aacaggccat taccctaatt gcaggacgtt ctcggattga atgttctgga
     2821 aatattgata tgaccactat tagccgtttt cgtggtttag cgattgatta cgtctccagt
     2881 ggtagtttaa cccatagtgc taagagtctt gatttttcca tgaaggggtt aacctacctt
     2941 gatgtctaag ttgtaaaata aactaacttt ttaaaggatg tctttcctct agaacgagtt
     3001 ttatgtcaga tagtttaaac gcctcttcaa atatagtaaa atgaaccaaa aatagtacac
     3061 aatgtggtat aatctt
//
LOCUS       DAVKDZ010000142         3016 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000142 DAVKDZ010000000
VERSION     DAVKDZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.144"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(<1..419)
                     /locus_tag="VWZ30_002143"
     CDS             complement(<1..419)
                     /locus_tag="VWZ30_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9335786.1"
                     /translation="MENFGAVLKDIRISKNFRLKDLSCNEISESTISRFENGITKLSI
                     NHFYILLNRLGISFSEFEELVHCYYSKKECLFEELEHAVNSSDIFLLQELVDKIELKQ
                     KQEKSLCNYHIKLIAEQQINRLANLPYNSSKCNELIK"
     gene            513..1733
                     /locus_tag="VWZ30_002144"
     CDS             513..1733
                     /locus_tag="VWZ30_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEU9335787.1"
                     /translation="MKKILLSTVALLSLVASLLANNPVSAQESSSQATYSKSSGSWIK
                     SGNRWWYKHSDGSYTTNGWEKINGTWYYFDSEGWMKTGWIKEYGKWYYLDDSGAMKTG
                     WCLVSGSWYYLNSSGVMQTGLQTINGKQYYLAAGGAMQTGWHNIGDDTYFFANSGENQ
                     NINRRALVLGETSTRAVPIADVNAMEKVFSNQNFSEVVRFPDRTKSEIIAKMQELFES
                     SSEGDVNYLYFTCHGGRDGRIYIGSDGLAFSGWELASVLKQYKGKFVVMLDCCHAGTI
                     ISKDNTGEGNEEASTEYFDLDEFVSGFSNMDGNEKSGEMIDSKFLVLCSSRGAEYSSG
                     GSLSLATKYWSLGSGWNPLQNSQAYLAADQNNNRRITLNELYTYSREQVLKQNSNQHI
                     EVYPDNSQFVLFKK"
     gene            1746..2864
                     /locus_tag="VWZ30_002145"
     CDS             1746..2864
                     /locus_tag="VWZ30_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9335788.1"
                     /translation="MKVSKKITLFSLSFAGFVLLTLPQAGKAFELKEDWAFKGGIRYE
                     NGKVSKINNGYEVNIKVLDLPSTSAIEWTVRLNGEKQNTNFLAEERTVSKTEDKGRFL
                     HFYIPYGYRGDIVVEAKSGNEVKTWSTKVVDDVYSDSAKSGYFILDGEQILESSWDSV
                     NESYIATLPTVTSGKTVVAWREEGTLNLIKPGRIVRQYNSSGSYVELSPIFETASWLK
                     SNQNWYYQKQGQLVQNSWIKDQGSWYAFKSSGAMISADWLYDNGSWYYLKDSGSMVTG
                     WLKNGGSWYYLNKSGSMATGWIKDSGTWYYLKNSGSMATGWVKDSGSWYYLKNSGSMA
                     TGWVKDNGKWYYLASSGNMLRNTRTPDGYYVDGSGAWK"
BASE COUNT         1018 a          405 c          617 g          976 t
ORIGIN      
        1 tattttatta attcattaca tttagatgaa ttataaggaa ggtttgcaag acgattaatc
       61 tgttgttcag caatcaattt tatgtgataa ttgcataaac ttttttcttg cttttgtttt
      121 aactctattt tatccactaa ttcttgaagt aaaaatatat ctgaagaatt tacagcatgt
      181 tctaattctt caaaaagaca ttcttttttg gaataataac aatgaactaa ttcttcaaat
      241 tctgaaaatg atattccaag acggtttaac agaatataaa aatgattaat agataatttt
      301 gtaatgccat tttcgaaacg agaaatagtt gattcactaa tttcattaca tgataaatct
      361 ttaaggcgaa aattttttga aatacgaata tcctttaaaa ctgctccgaa attttccata
      421 atgttcctct tccatttctg caaaaaataa aacgcttgtt ttttatttat gatatgatta
      481 tacaaaaata aaatagaaag gacaataata tcatgaaaaa aattttattg tcaacagttg
      541 ctttattaag cttagtagca tctctgctag caaataatcc agtttctgct caagaatcat
      601 cctctcaagc aacttatagt aagtcaagtg gaagttggat aaaatctggt aaccgttggt
      661 ggtataagca ttctgatggt tcttatacta caaatggatg ggaaaaaata aatggtacct
      721 ggtactattt tgatagtgaa ggttggatga agacaggttg gattaaagaa tatgggaaat
      781 ggtattacct agatgatagt ggtgctatga aaactggatg gtgtttggtt tctggtagct
      841 ggtattattt aaatagtagc ggtgtaatgc aaacgggact acagactatc aatggaaaac
      901 aatattattt agctgccggc ggtgctatgc agacaggttg gcacaatatc ggagatgata
      961 cctatttctt tgctaatagt ggggaaaatc aaaatatcaa tcgtcgtgct ttagttcttg
     1021 gtgaaacatc aacgagagca gtacctattg cagatgttaa tgctatggaa aaggttttta
     1081 gtaaccaaaa ctttagtgaa gttgttcgtt tcccagatag aacaaaatcc gagataattg
     1141 caaaaatgca agagcttttt gaatcttcta gcgaaggcga tgtgaactat ctttacttta
     1201 cttgtcatgg aggaagagat ggaagaatct atattggtag tgatggccta gcattttctg
     1261 gttgggaatt ggcgtccgtt ttgaaacaat acaaaggtaa gtttgttgtt atgctggatt
     1321 gttgtcatgc tggaacaatt atttctaaag ataatacagg tgaaggcaat gaagaagcct
     1381 ctacggaata ttttgattta gatgaatttg tatcaggatt ttctaatatg gatggtaacg
     1441 aaaaatcagg tgagatgatt gactctaaat ttttagtatt atgctcatca agaggtgcag
     1501 aatactctag tggtggatca ttaagtttag cgactaaata ttggtcttta ggttcaggat
     1561 ggaatcctct acagaattca caagcatatt tagcagcaga ccaaaataat aatcgcagaa
     1621 tcacattaaa tgaattgtat acatactctc gtgaacaagt tttgaaacaa aattctaacc
     1681 agcatattga agtttatcct gacaacagtc aatttgtgtt atttaagaaa taagggggca
     1741 ataaaatgaa agtatcaaaa aaaattacac tatttagttt gtcttttgca ggttttgttt
     1801 tattgacttt acctcaagca ggaaaggctt ttgaacttaa agaagactgg gcatttaaag
     1861 gtggcattcg atacgagaat gggaaagtca gcaaaattaa taatggatat gaagtaaata
     1921 ttaaagtgtt agatttacct agtactagcg caatcgaatg gacagttaga ttgaatggag
     1981 aaaagcaaaa tactaacttc ttagcggagg aaagaactgt atctaaaact gaagataagg
     2041 gacgtttctt gcacttttat atcccctatg gatatcgtgg ggatattgta gtagaggcta
     2101 agagtggaaa cgaagtgaag acttggtcta ctaaggtagt tgacgatgtt tattcagatt
     2161 ctgctaagag tggctacttt attctcgatg gggaacaaat cttagaaagt tcatgggatt
     2221 ccgtaaatga gtcttatatt gcaacgcttc caactgtaac atcaggaaaa actgttgttg
     2281 cttggcgtga agaaggaact cttaatttaa ttaaaccagg aagaattgtt cggcaatata
     2341 atagcagtgg atcatatgta gaactttctc caatctttga aactgctagt tggctaaaat
     2401 caaaccaaaa ctggtactat caaaaacaag gtcaattagt tcaaaattct tggatcaagg
     2461 atcagggaag ctggtatgcc tttaagtcat caggagccat gatttcagct gattggttgt
     2521 atgataatgg ttcttggtat tacctaaagg actcaggttc tatggtaaca ggatggctta
     2581 aaaatggagg ctcttggtat tacctaaaca agtcaggttc aatggctaca ggttggatta
     2641 aagatagtgg aacttggtat tatctaaaga attcaggctc tatggcgaca ggctgggtga
     2701 aagatagtgg ctcttggtat tatctaaaga actcaggttc aatggcaaca ggttgggtca
     2761 aagataatgg taagtggtac tatctcgcat catctggtaa tatgcttcgc aatacacgca
     2821 caccagatgg ctattatgta gatggcagtg gcgcttggaa ataaattttt aaaagagaaa
     2881 atctactgag aaggtagatt ttctttttag ttaatgaata tagaataaag tagatcagga
     2941 ctggtctatt ttattttgta actattaatg aatagtctta atgatgaagt tattttctga
     3001 agatatttaa ttgtgt
//
LOCUS       DAVKDZ010000143         2968 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000143 DAVKDZ010000000
VERSION     DAVKDZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.145"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(48..245)
                     /locus_tag="VWZ30_002146"
     CDS             complement(48..245)
                     /locus_tag="VWZ30_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9335789.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(320..1096)
                     /locus_tag="VWZ30_002147"
     CDS             complement(320..1096)
                     /locus_tag="VWZ30_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEU9335790.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            1218..1964
                     /locus_tag="VWZ30_002148"
     CDS             1218..1964
                     /locus_tag="VWZ30_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9335791.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            1977..2957
                     /locus_tag="VWZ30_002149"
     CDS             1977..2957
                     /locus_tag="VWZ30_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU9335792.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELIKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
BASE COUNT         1061 a          364 c          558 g          985 t
ORIGIN      
        1 tcaattcact atattagagt aaaattctct acaaaaagaa gaatagccta tttgactatt
       61 cttccgagtg atttcaattt ctttggggaa atatggagat actttttaaa tactttggca
      121 aaatatttat agtctgaaaa tccaacttgg tcagagatag cgcttaaagg ggagttggga
      181 gattcagcca tcttatctat agcttgtttc aggcgatatt gtaagatata ttcatttaga
      241 gtcatgggga agtcttcttt gataacctta tatagatgct tgttatctgc aaatagctag
      301 agcatttcaa ggaaataaac tagaaataac aattaatatg gtggttcaga aatacctttt
      361 gataatcaat aagatcttca ggatgaagtg cgttgattcc atccatttca tatttccgat
      421 ttaataaacg atatttgttt tcaccaaatt gatgaaaagg gagtagttga acttggtcga
      481 tatttaatga gttaaataga gtagcgaatt tttctgcatc ctctaaacta ttgttaaaat
      541 taggaataac tgggattctt aaaacgatag ttttattttg agaaaaagca taatgaatgt
      601 ttttaataat catttgatta aaaaccccag tcactttttt atgttttata gaattataat
      661 gttttaggtc tgtgtagata aaatccacat attgaattaa atcgataaat ttttcatgat
      721 caacaaaggc agtagtttca atggcagtgt gtatgtgatg ttctttagct gattttaaga
      781 tggctttagc aaattcaaac tgagcaaata tttcacctcc tgataaagtt aaacctccac
      841 cggattcttc gtaaaattct ttgtctttta atacctctgt aataatttct tctacagtct
      901 tttcttcacc gactaaggtg aatttctctc gttgagcatc tttcattttt tcaggtttca
      961 ttctttgaga ttcaggatta gaacaccatg gacagcgcag aggacatcct tttaaaaaaa
     1021 cagttgtacg aatacccggg ccgtcatgaa ttgaaaagtg ttgaatatta aaaataattc
     1081 ctttagatat ttccataaga gttcctcctc ttcataagtc tatcctatca caaaaacgaa
     1141 agaaaaacaa ttacgaacga aaggttaatt tgttgttttt tgctttttat tttggtagaa
     1201 taaaacgggg gtgtaaaatg aaaagattgg aacaaattat taaattagta tcagaatatg
     1261 aaaagatcga cgttaataca ttatcggaaa aattaaatgt atcgaaagta acgattagaa
     1321 aagatttaga taaattagag tcaaaaggtt tattacacag agagcatgga tatgctgtat
     1381 taaatagtgg agatgactta aatgtgcgtt tgtcaattaa ttatgaaatt aagagaaaaa
     1441 ttgttcagga agcagtaaaa ttggtgtcag ataatgaaac aataatgata gaatctggat
     1501 cgacctgtgc tttacttgct gaggaaattt gcaagcaaaa aagaaatgtt acgattgtaa
     1561 caaattcatt ttttatagca aattttgtga gagcttatga ttcatgtcgt attattgttc
     1621 ttggtggaga gtttcagaag gattcacagg ttactgtggg acctttatta aaagaaatga
     1681 tacagacttt tcatgtgcat caagcttttg ttgggacaga tggttacgat aaagagatgg
     1741 gctttacagg aaaagattta atgcgcagtg aggtagttca atatatttca gcagcatcgg
     1801 ataaagtcat tgtactaact gactcaagta aatttgataa aaggggtaca gtaagaagat
     1861 ttgctttaag tcaagtctat gaagtaataa cagacgaaaa actttctaaa caaaatatag
     1921 ctacattaga aaatgctggg ataatggtta aggtagtttc gtaagaggtt aagtgtatga
     1981 atcaagatag gaataaactg ctctctaaaa ttgcttatct gtattatatt gaaaacttaa
     2041 atcagtcaca aatagcagca aaattaggaa tttatagaac ctctattagt agaatgttaa
     2101 cagaagcaag gaatgcagga attgttaaaa ttgaaataga gaattttgat accaatatgt
     2161 ttaagttgga aaattatgta aaagaaaaat atggtctgga aagtttagaa attattccaa
     2221 atgaatttga tgataatcca acaattttat ctgaaagaat ttctcaagtt gcagcaggtg
     2281 tccttaggaa tctaattgat gataatatga aaattggctt ttcttggggg aaaagtttaa
     2341 gtaatttagt agatttaatt cacagtaaaa gtgtccgaaa tgttcacttc tatcctctag
     2401 caggtggtcc tagtcacata cacgctaaat accatgtgaa tacactgatt tatgaaatgt
     2461 ctagaaaatt tcatggagag tgtacattta tgaatgcaac gattgtgcaa gaaaataaat
     2521 tgttagcaga tggtattttg caatcaagat attttgaaaa tttgaaaaat agttggaaag
     2581 atttagatat agctgtagtc ggaattggtg attttagcaa taaaggaaaa catcaatggt
     2641 tagacatgct tacagaggat gactttaaag aattgatcaa agttaaaact gtaggagaaa
     2701 tttgttgtcg attttttgat tcaaaaggta aagaagtcta tgaaaactta caggaaagaa
     2761 cgatagcaat ctctttggaa gatttaaaaa atattcctca aagtttagct gttgcttacg
     2821 gtgatacgaa agtatcttcg attctttctg tcttgcgtgc taatttagta aatcatttga
     2881 ttacagacaa aaatacaatt ttaaaagttt tggaagaaga tggggatttg acttttagag
     2941 agattctagg tgagtgaaaa tgatagac
//
LOCUS       DAVKDZ010000144         2900 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000144 DAVKDZ010000000
VERSION     DAVKDZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.146"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(84..773)
                     /locus_tag="VWZ30_002150"
     CDS             complement(84..773)
                     /locus_tag="VWZ30_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473604.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9335793.1"
                     /translation="MKGCYAHVWKKHRDNGEKNYRYSICKYHFGAASVAVVALMFFAN
                     GAVAASETITPKTAGDVVKADSDSNADGNPESSDEGDSKKALTDQPAELKSADEPKGQ
                     EAPVEESDSVQAAAESKTTGVESNSAQPETNPAPQAEGEGKQDQSTPVANRTAAESIQ
                     STLEALLANLTLDSMKALHTEVEEGLAKAKTVLENPKATQAQVDEQVKLMEDLTRRVR
                     EACLLRFFFLK"
     gene            complement(1155..1618)
                     /gene="tnpA"
                     /locus_tag="VWZ30_002151"
                     /pseudo
     CDS             complement(1155..1618)
                     /gene="tnpA"
                     /locus_tag="VWZ30_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1890..2099)
                     /locus_tag="VWZ30_002152"
     CDS             complement(1890..2099)
                     /locus_tag="VWZ30_002152"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335794.1"
                     /translation="MFSQLRMREEQALLAQDYALETARVEGIEQGLERGKLFAFLDMV
                     RQGLLTPEVASEQLCMTVAEFEEFL"
     gene            complement(2092..2208)
                     /locus_tag="VWZ30_002153"
     CDS             complement(2092..2208)
                     /locus_tag="VWZ30_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9335795.1"
                     /translation="MVGVFRQQALYPATRASHQPSRPTAGLQELVRGGQENV"
     gene            complement(2254..>2900)
                     /locus_tag="VWZ30_002154"
     CDS             complement(2254..>2900)
                     /locus_tag="VWZ30_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9335796.1"
                     /translation="QREGDTHHSYKHIAPVYAIAIVDSNYFQDDFAFHSFSMSEDTIG
                     EVLTITTNGQENHLVKMAFLELKKYRETSKDEVRKPWLEFFGNKPFTQEPERAISQAD
                     QLLDYKSWSEEDRKMFSQLRMREEQALLAHDYALEQAEEKGLERGRAEGLERGRAEGI
                     EQGIEKGLEQGLERGKLFAFLDMVRQGLLTSEVASEQLGMTVAEFETLLKKHHK"
BASE COUNT          759 a          678 c          525 g          938 t
ORIGIN      
        1 actattttta caaatttgaa cagcaaaacg ctaacttttt tcagccgtct tgtcacttat
       61 ttttataggt ggtttttgag ctgctatttc agaaaaaaaa aacgaagcaa gcaagcttcc
      121 ctgacccggc gagtcaggtc ttccatgagt ttgacttgct catcaacctg tgcttgcgtc
      181 gcttttggat tctctaagac cgtcttagcc tttgcgagac cttcttccac ttcagtgtga
      241 agagccttca tggagtctaa ggtcagattt gccaacaaag cttctagggt gctttgtatc
      301 gattcggctg ctgtcctgtt ggcaacagga gtcgactggt cttgttttcc ttctccctct
      361 gcttgaggag ccggatttgt ctctggctga gcagagttag attctactcc agtcgtttta
      421 gactcagcag ctgcttgtac tgaatcgctt tcctcaactg gcgcttcttg ccctttcggc
      481 tcgtcggctg atttgagttc agctggctgg tcagtcagag ctttcttgga gtctccctca
      541 tccgaggact ctggatttcc atctgcgttg ctatcagagt cagccttaac gacatcacct
      601 gccgtttttg gagtaattgt ctcactcgcc gccacagccc cattcgcaaa aaacatcaag
      661 gccacaacag caacgctggc agcaccaaag tggtacttac aaatggaata acggtaattt
      721 ttttcaccat tatccctatg ttttttccaa acatgagcat agcatccttt cattgtatca
      781 caggtcaact ttcctcaaaa gagtacacaa aaacccgtaa cagataatat gtcaacttta
      841 tagtaatact tttctagaaa gaaatcaagt ataaagctag atgaagataa gattagcata
      901 agattaaaaa tgcagcaaaa acaccgatat aacaacattt ttctgtaatt atatatatat
      961 gcacaccttt atataacaat cttattatgg ataatatttt aaatttaata taaatcatga
     1021 ccacccgtct aacgggtggt ttgtaccagg gctataagcc caattaccag ccagcgccta
     1081 aagacgctgg ctttcacttt gttcaatcct tattgctctt gactcgtcac ttgcctctta
     1141 aagaggcttt ggtattactt accactatcc ctaaagggat cctcatattc ttttacattc
     1201 aatttatcta gtgctatatc atgcttttcc tgttctggta tatatttctt aattgtagct
     1261 tcattgactc ccaccgtact cacataataa ccttccgccc agaaatgccg atttccaaat
     1321 ttgtacttga gattggcgtg tttgtcaaac atcatgagtg cactttttcc ttttaaataa
     1381 cccatgaaac tcgaaacact tatactaggt gtaatactta ctaacatatg tacatggtct
     1441 ggcattaagt gaccctcgat aatttcaact cctttataac tacacaagcg atgaaatatt
     1501 tcgcctaaac tacttcgata ttgattatag ataacttttc gtctatactt aggggtgaac
     1561 acaatgtgat agaacagtgc cactttgtgt gcgataaact atgagccttt tgtgccatgt
     1621 ttttctcctt tcgctttaca attggcttga acacctttat tgtatcgcgt ttggagtttt
     1681 ttgggtataa ccttcgacgc gcacctaacg gagcgagacg gactaatagt cacataataa
     1741 aaaacagatt ctatctatag aaagataaaa tctgccattt tttctatata aaatcagcaa
     1801 atccattctg ctgaaaatcg tgaaatatga tgtgaatttc cgaccactct gacaggattt
     1861 tgagggaaaa gctagatata ttccctaact tacaaaaact cctcaaactc agcgacagtc
     1921 atacacaatt gctcactcgc aacctcaggt gtcagaagac cttggcgaac catatctaag
     1981 aaggcaaaaa gttttccacg ttctaaacct tgttcaatac cttcgactct agctgtttcc
     2041 aaggcatagt cctgtgctaa taaggcttgt tcttctcgca tacgtagttg actaaacatt
     2101 ttcctgtcct cctcggacca gctcttgtaa tccagcagtt ggtctgcttg gctgatggct
     2161 cgctcgggtt gctgggtaaa gggcttgttg ccgaaaaact ccaaccacgg tttgcgaacc
     2221 tcgtctttgc tggtttctct gtatttttag gtcttattta tgatgttttt tcaatagtgt
     2281 ctcaaactca gctacggtca tgcccaattg ttcactcgca acctcagatg tcagaagacc
     2341 ttggcgaacc atatccagaa aggcaaaaag ttttccacgt tcaagtcctt gttctaaacc
     2401 tttttcgata ccttgttcaa tcccttcagc tcgtccacgt tcaagtcctt ctgcacgacc
     2461 tctctccaac cctttttcct cagcttgttc caaggcatag tcatgagcca ataatgcctg
     2521 ttcttctcgc atacgtagtt gactaaacat ttttctgtcc tcctcggacc agctcttgta
     2581 gtccagcagt tggtctgctt ggctgatggc tcgctcaggt tcttgggtaa agggtttgtt
     2641 cccgaaaaac tccaaccacg gcttacgaac ctcgtctttg ctggtttctc tgtatttttt
     2701 tagttccaag aatgccatct tgaccaaatg gttttcttga ccgttagttg tgatagttaa
     2761 gacctcacct atcgtgtctt cgctcatact aaaactgtga aaggcaaaat catcttggaa
     2821 gtaattacta tctacgatgg caatagcata aactggtgcg atatgtttgt aactatggtg
     2881 tgtatcacct tctcgttggc
//
LOCUS       DAVKDZ010000145         2859 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000145 DAVKDZ010000000
VERSION     DAVKDZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.147"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     regulatory      51..139
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            354..1022
                     /locus_tag="VWZ30_002155"
     CDS             354..1022
                     /locus_tag="VWZ30_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEU9335797.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     gene            1249..2031
                     /gene="thiM"
                     /locus_tag="VWZ30_002156"
     CDS             1249..2031
                     /gene="thiM"
                     /locus_tag="VWZ30_002156"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9335798.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            2033..2662
                     /locus_tag="VWZ30_002157"
     CDS             2033..2662
                     /locus_tag="VWZ30_002157"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9335799.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
BASE COUNT          948 a          414 c          625 g          872 t
ORIGIN      
        1 atcttgctag ggctttcatt ttttgctata ctggttcagt aattaaataa tagagacatt
       61 tggggtgctt taggctgaga taatacccat tgaacctgat acagttaaga ctggcgaagg
      121 gaaatgtgaa cagttttttc gtatggtgaa atgaacggtc taatcttgta agtcaactct
      181 aattcttgat tgtaattgga gttttttgtt tatataaact ggataggctt gtttagttag
      241 ctctctgcaa atgctctcga tgtgctttaa aaggatcaag tggaattacg attttgcttg
      301 atatttcttg gtgttttatt aattactaac aagtaaagag aggagaagta aatatggaaa
      361 cacaagacta tgcatttcag ccaggtttga ctgttggaga attattaaaa agcagtcaga
      421 aggattggca ggctgcaatc aatcatcgtt ttgttaagga actttttgcg gggacaattg
      481 agaataaggt cttaaaggac tatctgattc aagattatca cttctttgat gccttcttat
      541 ccatgctggg tgcctgcgta gctcacgcag ataaacttga atccaagctt cgttttgcca
      601 agcaactagg ctttcttgaa gcagatgaag atggttattt ccaaaaggct tttaaagagt
      661 taaaagtagc agagaatgac tatctagaag tgactttgca ccctgtaaca aaagcctttc
      721 aggatctaat gtattcggca gtggcttcat cagactatgc ccatcttttg gttatgctgg
      781 tcattgcaga aggtctctat ttagactggg gttctaaaga tttggctcta cctgaagtct
      841 atattcattc ggaatggatc aatctccaca gaggtccttt ctttgcagag tgggttcaat
      901 ttctggttga cgaactcaat cgtgtcggta aaaatcgaga agatttgaca gaacttcagc
      961 aacgctggaa tcaagcagtc gctttagaat tagctttttt tgatattggt tacgatgtct
     1021 agagaaggct catgccacaa cctcaaaaca gtgttttgag ctgacttcgt cagtcttatc
     1081 tacaggttca aagcagtgtt ttgagcaacc tgcggttagc ctcctagttt gctctttgat
     1141 tttcattgag tataaatcat tcaacgtaaa tgaaaaaaat ttcttaacga aagatagaga
     1201 taaatcgaaa tcagtagatc gtataaagtg aaaaggaagg atttcaatat gacaagttta
     1261 aaattattaa aagaaaaagc accattggtc atttgcatca ccaatgatgt agtaaaaaat
     1321 ttcacagcaa atggattagt agcactgggc gcctcaccag ccatgagtga gtttccagca
     1381 gatttagagg atttgttaaa gtatgctggt ggtttattaa taaacatagg aacattgaca
     1441 gatgaaaatt ggaaattata ccaagctgct ctgaaaattg cggagaaata taatgtccca
     1501 gcagttttag atcctgtagc ctgtggagca ggagaatata gaaaaaaagt agcagatgat
     1561 ctaatcaaca attataaact agcagcgatt agaggaaatg ctggcgagat tgcctcttta
     1621 gtaggaatag atgtggcatc taaaggagta gatagtgcgg gcgtagataa tattgacgaa
     1681 attgctctag cagcaaatga gaagttcaat attccaatag tagtaacagg tgaagtggat
     1741 gccattgctg ttaatggaga agtggtaacg attcataatg gtagtgctat gatgccgaaa
     1801 gtcattggga caggatgctt attaggagct gtagtagcaa gttttatcgg actggaaaaa
     1861 ggtcaagaat taaaatcatt agaaacagca atgttagttt acaatatcgc tggagaaatg
     1921 gcagaaaaac gtccaaatgg acatcttcct gggacattta aagttgaatt tataaatgcc
     1981 ttatacgaga ttacagatga agatgtaaag gaattcaaaa gagtgaagta agatgtttca
     2041 taaagaatta ctaaaactat attttatttg tggaacgact acttgccaag gaaaaaatct
     2101 atatacagtc gttgaggaag ccttaaaagg tggtataacc ttatttcaat ttcgagaaaa
     2161 aggtgagggt gctttagaag gcttagaaaa acttgagcta gctatccaaa ttaaagaact
     2221 ttgtaaaaaa tacaatgttc catttatcgt taatgacgat atagatttag caatggaaat
     2281 tgacgccgat ggcgtacatg taggtcaaga tgatattggt gttgatgaaa ttagaaaatt
     2341 gatgccagat aaaataattg gtctttctat aagaaacgaa gaagaatttc aacaatcaaa
     2401 agttgaatat gtagattatg taggtgttgg gcctgtattt gatacccagt caaaagatga
     2461 tgctggtggt gctataggtt atgaaggtct tgaattgatg agaaaactat tgccacaaat
     2521 gcctttagtt gcaattggtg ggatacagac gaaacatatt aaagacatta taaagaccaa
     2581 tatggatggc gtttcaatca tttcggcaat atcgtatgca aaaaatatag aaaaaactgt
     2641 tcgggaaatg agtgaacaat agatatctat ccttggattc taagtaagta ggattttcct
     2701 tttttaaaaa gctatgtcca gaaagctatg tccagattca gcttgaatgg ctaattcatc
     2761 aaacaggtca tttgtttttt gcaaaagaaa gatctgctgt ctttgtgagt gcaaggctgt
     2821 atttccagtt caatttcttg aactagtata aaatgtgtg
//
LOCUS       DAVKDZ010000146         2780 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000146 DAVKDZ010000000
VERSION     DAVKDZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.148"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            83..1087
                     /locus_tag="VWZ30_002158"
     CDS             83..1087
                     /locus_tag="VWZ30_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9335800.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKNEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKVYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLVSVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            1156..2022
                     /locus_tag="VWZ30_002159"
     CDS             1156..2022
                     /locus_tag="VWZ30_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9335801.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGIKQIQDVLPFDSDLNRLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            2019..2777
                     /locus_tag="VWZ30_002160"
     CDS             2019..2777
                     /locus_tag="VWZ30_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9335802.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSSEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGQIIQDLNQEEKAKMKISDYYQLFE"
BASE COUNT          867 a          490 c          612 g          811 t
ORIGIN      
        1 aaaccttgca atagtcaagt gataacgaat aaaacttggg attgaaatat ccccatgatt
       61 atatattacg aggagaaaca gaatgaaaaa taaacgttta attggaatta tcgctgcatt
      121 agcagtctta gtagcaggaa gcttgattta ttcttcaatg aataaatcag aagctcagaa
      181 taataagaat gagaagaaaa taaccaagat tggtgtgctt caatttgtga gccatccatc
      241 ccttgatttg atttataaag ggatccaaga tggacttgca gaagaaggat ataaagatga
      301 tcaagttaaa attgatttta tgaactcaga aggtgatcaa agtaaggttg cgacaatgag
      361 taaacaattg gttgcaaatg ggaatgacct tgtggttggt atcgcaacac cagcagccca
      421 agggttggct agtgcaacaa aagacctacc ggttatcatg gccgctatta cagacccaat
      481 tggtgctaac ttggttaaag atttgaaaaa accaggtggc aacgttacag gggtatctga
      541 ccacaatcca gctcaacaac aagttgaact catcaaggct ctgacaccga atgtgaaaac
      601 aatcggagct ctttactcaa gtagcgaaga caattcaaaa acacaggtcg aagaatttaa
      661 ggtttatgct gaaaaagcag gtctgacagt ggaaacattt gcagttcctt caacaaatga
      721 aattgcctca actgtcactg ttatgactag caaggtagat gctatttggg ttccaattga
      781 taacaccatt gcatcaggat ttccaacggt tgtctctagc aatcaaagtt ctaagaaacc
      841 aatttatccc agtgcgacag ctatggtaga agtaggtggt ttggtatcag ttgtaattga
      901 ccaacatgac cttggtgtag caacaggtaa aatgattgtg caagtcttga aaggtgcaaa
      961 accagccgat accccagtca atgtcttttc aactggtaag tcagtcatca ataaaaaaat
     1021 agcacaagaa ctaggtatta ctattcctga gtctgttctc aaagaagcag gacaagtcat
     1081 cgaataaata aggtagggag gaggagactc tttccatttt ttaaaaagat gaatatagaa
     1141 aggattaaat aaaatatgat tgtttccatt atttctcaag gatttgtctg ggctattcta
     1201 ggtctgggaa tctttatgac atttaggatt ttaaactttc cagatatgac gacagaaggt
     1261 tccttccctc ttgggggagc tgttgctgtc actttgataa ccaaaggcgt gaacccattt
     1321 ttagcgacac ttgttgctgt aggagcaggt tgtttggctg gaatggcagc aggccttctt
     1381 tatacaaaag ggaagatccc aaccttgctc tcagggattt tggtgatgac ttcttgtcac
     1441 tcaatcatgc tcttgattat gggacgtgcg aatttaggcc tgcttggaat caagcaaatt
     1501 caggatgttt tgccttttga ttcggatttg aatcgactct tgacaggtct catctttgtg
     1561 agtattgtta ttgctctcat gctctttttc ttggacacta aactcggaca agcctatatt
     1621 gctacagggg ataatcctga tatggctaga agtttcggga ttcatactgg acgcatggag
     1681 ctcatgggct tggtcttatc aaatggtgtg attgcccttg caggtgccct cattgctcag
     1741 caagaaggct atgccgatgt gtctcgaggg atcggggtta tcgttgtggg gcttgcaagt
     1801 ttgattattg gagaagttat tttcaagagt ttgagcttgg cagagcgttt ggttactatc
     1861 gttgtaggtt ctatcgctta tcaattttta gtgtgggcag ttatcgcact tggctttaat
     1921 acaagttacc ttcgtttata cagtgccttg attttagcag tctgcctcat gattccaaca
     1981 tttaagcaaa caatcttgaa aggagccaag ttaagcaaat gacagcaatt gtagaattaa
     2041 aaaatgcaac caaaatcgtt aaaaatggct ttgatgaaga aaagattatt ttaaatgatg
     2101 tttccttaga aatttttgaa cgggacttta tcacgatttt gggcggaaat ggtgctggaa
     2161 aatcaaccct ctttaacact atagcaggga ccttatcact aactagtgga actatccgta
     2221 ttttaggtga agatctcact aagttttcat ccgagaagcg tgccaagtac ctgtctcgtg
     2281 tcttccaaga tccaaagatg ggaacagctc cccgtatgac ggtcgctgaa aatcttttaa
     2341 tcgccaagtt tcgtggtgaa aagcgtggat tgttaccacg acgcttgact agctataagg
     2401 atgaatttca ggcaaccatt gaaaaagtag gaaatggtct tgagaaacac ttgaatacac
     2461 cgattgagtt cttatcaggt ggacaaagac aggctttgag tctcttgatg gcaaccttga
     2521 agcgacctga attactcttg ttagacgagc atactgcagc tcttgatcca aagacaagtg
     2581 ttgccctcat ggaattgaca gatgaatttg tcaagaagga tcaattgaca gcgcttatga
     2641 ttactcatca tatggaagat gctcttaagt atggcaatcg cttaattgtc atgaaagaag
     2701 gacaaattat tcaagatttg aaccaagaag aaaaagcaaa aatgaaaatc tctgattatt
     2761 atcaactatt tgaataaagt
//
LOCUS       DAVKDZ010000147         2745 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000147 DAVKDZ010000000
VERSION     DAVKDZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.149"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            706..2628
                     /gene="ltrA"
                     /locus_tag="VWZ30_002161"
     CDS             706..2628
                     /gene="ltrA"
                     /locus_tag="VWZ30_002161"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEU9335803.1"
                     /translation="MTKPKNDDKLLKHQKLRYAEYYGMTEIFDDLYSKSQNGFSFKNL
                     MNIITSPENILLAYRTIKRNGGSQTEGVDGVTIKDLENLTQEDFISKVQRRFQYYRPR
                     KVRRVEIPKPNGKKRPLGIPSMWDRVAQQCILQVLEPICEAKFYKHSYGFRPLRSADN
                     AIMDCMYRMNKSHMTYVVDVDIKGFFNEVNHTKLMRQIWTLGIRDKQLLVIIRKMLKA
                     PIVLPNGKVTYPTKGTPQGGILSPLLANINLNEFDWWIGRQWEERDCKELVPQYNSKG
                     TRHKSHVYRKLRTSTTLKEVYIVRYADDFKIFCRNRNEAERTFKAVKLWLKERLNLPI
                     SEEKSQITNLRKKSSEFLGITLKLVSKNNRLVCFSHIASKAKKRIKHQLKQQMKLIQI
                     KGAKETIIREIQKYNSMVIGIHNYYSMATHVSKDLEEIHYQLYLSFKNRLQAKGLTKI
                     GEYKGKDKGLLPYLQSQNIRYLMGYPILPISYVKTRTLKGRNKNLNRYTPEGRKLIHK
                     QQESVETWKLKWLREHSIINKRATVEYNDNRISLFVAQKGKCAVTGQELDMDDIHCHH
                     KRPWLETKDDSYRNLIIVGRDIHRLIHATNEKVIKNLFQLLNLSDTGLTKLNQLRELA
                     GNSPLLKENLNLEQLC"
BASE COUNT         1004 a          425 c          577 g          739 t
ORIGIN      
        1 aaaaggttag acacgttctt gaaaaatcaa cctgataatc aggttaaatt tgaatagaat
       61 ggagaactct tatcttgaaa attggaatga aggggtaacg ccctgaaacg atttctctaa
      121 tactccgaca tgcgtttatc ttgtgcaaag gtaaaggttt gaagctcggt gaagtcggct
      181 gaaagatacc gtcattctta aggacttaag aatcgaaagt gttccagagg tggaatatgt
      241 atctgatagg tcgggagtca ttaaaaacta aatagggtga gaatgtgcat gagcactgac
      301 gaaccagcga atgtacgctc cgaaggcgag tacgtagaaa tgcgtataac aacgtaagtt
      361 gtttcaactt gaggaagagt aagaatcgac gatatgaaat tccttgttat gttacaggca
      421 tatcaaagtt gaaggggtat agctaggcac ctaagtttag tattgataaa gataagggaa
      481 cgtcgaaagc tagagatgtg gaggttgcac aaccagagaa acattggaaa gaaaatcata
      541 actttcctta atctctagtg agagtggtgg cacgaccttt gaaagtgttg taatgatact
      601 ggaggaacag ccactagtca atagtgtatt gttatgaact ttatcatttc atggattctt
      661 gtaagacata aaaggtcacg aatccaaaga gaggagtgat agaccatgac gaaaccaaaa
      721 aatgatgaca aactattaaa acatcaaaaa ctaaggtatg cggaatacta tggcatgacc
      781 gagatatttg atgatttata ttccaaaagt caaaatggat tcagttttaa gaacttaatg
      841 aacattatca catctcccga gaatatactg ctcgcttatc gtaccataaa gcgaaatggt
      901 ggtagtcaaa ccgaaggagt agatggtgtt accattaaag atttagaaaa tctgacccaa
      961 gaagacttta tctctaaagt tcaaagaaga tttcaatatt atagacccag aaaagtcaga
     1021 agagtagaga ttcctaagcc aaatgggaag aaacgaccac ttggcattcc ctcgatgtgg
     1081 gacagagtag ctcaacagtg tattttacaa gtgttggagc caatctgtga agcgaaattt
     1141 tacaaacaca gttatggatt tagaccctta aggtcggctg ataacgctat tatggactgt
     1201 atgtatcgta tgaataaaag tcatatgact tatgtcgttg acgttgatat taaaggcttt
     1261 tttaatgaag tgaatcatac taagttaatg cgtcaaatct ggacattagg tatcagggac
     1321 aaacaacttc tagtgattat tcgaaaaatg ttaaaagcac caattgttct tccgaatgga
     1381 aaagtgacat atccaactaa gggcacacca caaggaggta tcttatctcc actacttgcc
     1441 aatatcaatc tcaatgaatt tgattggtgg ataggacgac aatgggaaga aagagattgt
     1501 aaggaattag ttcctcaata taattccaag ggaacaagac acaagtcaca tgtttacaga
     1561 aagctgcgaa cttcaacgac gctaaaagaa gtttatattg tccgatatgc ggatgatttt
     1621 aaaatctttt gtcgaaatag gaatgaggca gagcgaacgt ttaaggctgt aaaattgtgg
     1681 ttaaaagaac gacttaacct ccctatttca gaggaaaaat ctcaaattac caatctgaga
     1741 aagaaaagca gtgaattcct tgggattact ttaaagctag tttctaagaa taatcgtttg
     1801 gtttgtttct ctcatattgc atcaaaagcc aagaaacgta ttaaacatca actgaaacaa
     1861 cagatgaaat tgatacaaat taaaggtgct aaagaaacaa ttattcgtga aatacagaag
     1921 tataatagta tggttatcgg tattcataac tattatagta tggcaacaca tgtgagtaag
     1981 gatttagaag aaattcatta tcagctttat ctctctttta aaaatagatt gcaggcgaaa
     2041 ggtctgacaa aaataggaga atacaaggga aaagataaag gacttctccc ctacctccaa
     2101 tctcaaaata ttcggtactt aatgggttat ccaattcttc caattagcta tgtcaagacg
     2161 agaacactaa aaggaagaaa taagaatctg aataggtata ctcccgaggg gagaaaattg
     2221 attcataaac aacaggaatc tgttgaaaca tggaaattaa aatggctgag agaacactca
     2281 attataaata agcgtgcaac agttgaatac aatgataatc gaatttcttt atttgtagct
     2341 caaaaaggga aatgtgctgt aaccggtcag gaattggata tggatgatat tcattgtcac
     2401 cataagagac cgtggttaga aacaaaggat gactcttata ggaaccttat cattgttgga
     2461 cgagatattc atcgcctgat tcacgcaaca aatgagaagg ttatcaaaaa tctctttcag
     2521 cttttgaatt tgagtgatac aggactgacc aaattaaatc agttgcgcga acttgcagga
     2581 aattcaccat tattgaaaga aaatttgaac ttagaacaat tatgttaatc atattaatac
     2641 agcacgataa agcaaaatcg tgataataca ctattgatgg aacgccgtgt gcggtgaaag
     2701 tcgcacgcac ggtgtgaagc gggggaaaag atggagatag tatca
//
LOCUS       DAVKDZ010000148         2739 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000148 DAVKDZ010000000
VERSION     DAVKDZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.150"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(149..913)
                     /locus_tag="VWZ30_002162"
     CDS             complement(149..913)
                     /locus_tag="VWZ30_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9335804.1"
                     /translation="MFKLSFHSIGHVVVRNYMSFRNLFKISIVPNLIAPLFYLLAMGF
                     GVGAYLTHVNGMLYRDFVITGLIAATAMSAATAETTVNAFIQYKIEKTYDAMLMTPIN
                     TNDIVVGQAIWSGIRAVIFGGIFWLISMLITAHFHVLMLLIPLILFIVGYLFGVLGLI
                     FTYLAPSREFLNYYNVLVIRPLYMFSDTFFPITSLPPILGDLTWFSPLYHATRMIRII
                     WGGEVTGLYLHLIWLIALALLITVLPMYMLHRRYYR"
     gene            complement(906..1592)
                     /locus_tag="VWZ30_002163"
     CDS             complement(906..1592)
                     /locus_tag="VWZ30_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9335805.1"
                     /translation="MLYLWGKERVKKLSVKNLKKQYGSYEAVKGISFELGEGECLGIL
                     GKNIETNRRKSKSCLGIVGQEDMLDMTLNVYDNLFAHGLCYGISSVELRKRIGSLLDF
                     VQLSKHAQKMVNQLSGGMRRRLVLARALINRPDIIILDEPTVGLDIQSRNIIWDKLLE
                     LKELGVSIIITSHYMNEIEYLTDRVLMLHQGTIKEEGTVEDLLIKYDADNLETLFLGL
                     TGTEKEDLHV"
     gene            complement(1562..2674)
                     /locus_tag="VWZ30_002164"
     CDS             complement(1562..2674)
                     /locus_tag="VWZ30_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEU9335806.1"
                     /translation="MNLPKIKDIHSVLFLKDKIRLGIGKDYGIEIENLDEKYTQLIKL
                     LDGQYTINEICSLVDSLSPNEVEESISLLDTLGYLEDNFVTTTELTSEELDRYSVNLN
                     FFNTLDTLSLTKYDYQLKLKKTRILLLGIGGIGSNICLALAELGIGHITAVDFDTVEA
                     SNLNRQILYSTSDIGKRKVEAAKESLRKFNPLIEFEVLDKEIGSKEDVDELIKTSKCD
                     IVVNVADYPTGYIDFWVNEACVQYNKLCLSAVVGKKNGKVYSIIPYKAACYYCQFLED
                     LEKIPEYAEELETVRTMTNSLELEMFRTPNGALGPACLFQGYFISFEILRYIFWGYEA
                     LLTFNKRFSIDFITFEQHFDELTKYPTCSICGGKKE"
BASE COUNT          957 a          585 c          394 g          803 t
ORIGIN      
        1 caatttctaa caatatttta aaaactgaac cgtactattt caacttccat aaattacaat
       61 attgataggc acagttccca tacctatcca aattcgaata acgtttagta taaattagaa
      121 tatgactaca acatcacaaa taattagact atctataata tctacgatgt aacatataca
      181 taggaagcac cgttatcaag agcgccaagg ctatcaacca gataagatga agatacaaac
      241 ctgtcacctc acctccccat ataatacgaa tcatgcgggt agcgtgatac aagggagaaa
      301 accaagtcaa atcaccaagt attgggggca aggaagtaat gggaaagaag gtatctgaaa
      361 acatataaag aggacggatt accaaaacat tgtagtaatt cagaaattcc ctagacggcg
      421 ccaagtaggt aaagattagt cccaaaacac caaacaaata acccactata aataaaatca
      481 gaggaatcaa taacatcaag acatgaaaat gcgctgtgat aagcatggaa atcagccaaa
      541 aaatcccacc gaaaatcact gcacgaatac ctgaccagat agcctgaccc accacaatat
      601 cattcgtatt gattggcgtc atcaacatag catcataagt cttttcaatc ttatactgga
      661 taaaggcatt gaccgttgtt tctgcggtgg ccgcagacat ggcagtcgca gcaattaaac
      721 ctgtaatgac aaaatcacgg tagagcatac cgttaacatg cgtcaaataa gcaccaactc
      781 caaatcccat tgctaaaaga taaaagaggg gggcgataag gttggggaca attgaaattt
      841 taaataaatt tctaaaactc atgtagttgc gtaccacaac gtgtccgatg gagtgaaatg
      901 ataacttaaa catgcaagtc ctccttctct gtcccagtca accccaaaaa aagtgtctca
      961 aggttgtcag catcatactt gatgagcaag tcttctacag ttccttcttc tttgatagtc
     1021 ccttggtgga gcatcagcac cctatctgtc aaatattcaa tctcattcat atagtgggaa
     1081 gtaataatga tggaaacacc taattccttc aactccaaaa gtttatccca aataatattg
     1141 cgggattgaa tgtctaaacc aactgtcggt tcatctaaaa taataatatc aggccgattg
     1201 atgagagctc ttgccaagac aagacgtcgt cgcatcccac ccgacagctg attcaccatc
     1261 ttttgcgcat gtttgctcag ttgaacaaaa tccaagagag aaccgattct cttcctaagt
     1321 tcgacagatg agataccata acatagaccg tgagcaaaaa gattatcata aacattcaag
     1381 gtcatgtcca gcatatcctc ctgccctaca atacccagac aagatttact cttacgacga
     1441 ttggtttcaa tatttttccc caagataccc aaacactctc cttctcccaa ttcaaagcta
     1501 attcccttga cagcttcata agaaccatat tgctttttta gatttttaac acttaacttc
     1561 ttcactcttt ctttccccca caaatagagc atgttggata ctttgttaat tcatcaaaat
     1621 gttgttcgaa tgttataaag tcaattgaga atcttttatt aaatgttagc aatgcttcgt
     1681 atccccaaaa aatatatctt aaaatttcaa acgaaataaa atacccttga aatagacaag
     1741 caggacctaa agcaccattc ggagttctga acatctctag ttccaatgaa tttgtcattg
     1801 tacgaacggt ctccaactct tcagcatact caggaatttt ttccaaatcc tctaaaaact
     1861 gacagtaata gcacgctgct ttatacggaa taattgaata tactttccca tttttcttac
     1921 caaccacagc agacagacat aatttattgt attgaacaca ggcttcattg acccaaaaat
     1981 caatatagcc tgttggataa tctgcaacat ttactacgat atcacatttt gaagttttta
     2041 ttaactcatc gacatcctct tttgaaccta tctctttatc aagtacctca aattcaatta
     2101 aaggattaaa ctttctaagc gactcttttg cagcctctac ttttcttttt ccaatatcag
     2161 atgtcgaata caaaatttgt ctattgagat tactagcttc gaccgtatca aaatcaactg
     2221 ctgtaatatg ccctatacct aattcagcca atgctagaca gatattactt cctataccac
     2281 caatacctaa gagcaaaata cgcgtctttt ttaattttaa ctgataatca tactttgtta
     2341 aagataatgt atctaaagta ttaaaaaagt taagatttac ggaatatcta tccaattctt
     2401 ctgaagttaa ttcagttgta gtaacaaaat tatcctccaa ataccctagt gtatctagta
     2461 aagaaatact ttcttctacc tcattagggg acaagctatc aactaagcta caaatttcat
     2521 tgattgtgta ttgtccatcg agaagcttaa tgagctgagt atacttctca tccagattct
     2581 caatctcaat accataatct tttcctatac ctaatctaat tttatccttt aaaaatagga
     2641 cactatgaat atctttaatt ttaggtaaat tcaattctat ttcctcctaa aatagacaaa
     2701 aagaatggaa aaatccattc acttttgaaa aaatgttga
//
LOCUS       DAVKDZ010000149         2095 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000149 DAVKDZ010000000
VERSION     DAVKDZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.151"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..1103
                     /locus_tag="VWZ30_002165"
     CDS             <1..1103
                     /locus_tag="VWZ30_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEU9335807.1"
                     /translation="TLVKPFEQNVTDKDLLDAVNADHKKSAKLKDGTSYPTTDGFHDI
                     DLTVTYEDGSMENVSVKYKVTDTAKADIDTAAAEKKTAIEKTPNLTPEEINVAKRQVD
                     EALAEAKRKVGEATDQAGINRAKQEGLSSIDLVLDQALVQSFGRTNGRRSRRTPPRRS
                     RRSVGFVGNSQTGTTPSAVDKSELRTLVEDLERRLKELEGLSPEAQSILREAQAALAN
                     DSLTAQELAKLLAKVRQALNSIQAGTSADKSPSTSNSNKEAESAKEPTNATDVPLYGV
                     FGAAALSLLGALLFAVARKKNSQLDKLLRELDQLLVELEASDKDKKGLGKAKKLAKKA
                     RTFVDSQQKDPQKEAELISEIKTTLSQLREGV"
     gene            complement(1205..>2095)
                     /locus_tag="VWZ30_002166"
     CDS             complement(1205..>2095)
                     /locus_tag="VWZ30_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9335808.1"
                     /translation="IAHQLSISTSTVIRKLNDFHFKHDFSCLPEIMAWDEYAFTKGKM
                     SFIAQDFNNLNIITVLEGRTQAIIRNHFLRYDRAVLCRVKIITMDMFIPYYDLARQLF
                     PCAKIVLDRFHIVQHLSRAMSRMRVQIMNQFHRKSHEYKAIKRYWKLIQQDSLKLSDK
                     RFYRPTFRMHLTNKEILDKLLSYSQDLKYHYQLYQLLLFHFQNKEPEKFFGLIEDNLK
                     QVHPIFQTVFKTFLKDKEKIVNALQLPYSNAKLEVTNNLIKLIKRNAFGFRNFENFKK
                     RIFIALNIKKERTKFVLSRA"
BASE COUNT          701 a          372 c          460 g          562 t
ORIGIN      
        1 ctacattagt caaaccattt gaacaaaatg ttacagataa agatttgtta gacgctgtaa
       61 atgctgacca caaaaaatct gctaagttga aagatggaac tagctatcct acaacagatg
      121 gtttccatga tattgatctt acagtgacat atgaggatgg tagtatggaa aatgtatcag
      181 tcaaatacaa agttacagat actgcaaaag ctgacattga taccgcagct gctgagaaga
      241 aaacagcgat tgagaagact ccaaacttaa ccccagaaga gattaacgtt gcgaaacgcc
      301 aggtagacga agctcttgca gaagcgaaac gtaaagtagg agaggcgaca gaccaggcag
      361 gaattaaccg agcgaagcaa gaaggcttat ctagtataga tctggttctg gatcaagctt
      421 tggttcaatc ttttggtaga actaatggta gaagaagtcg cagaactcct ccgcgccgct
      481 ctcgtagaag tgtaggattt gtagggaatt cccaaacagg tacaactcca tcggctgtgg
      541 acaagtctga acttcgtact ctggtagagg atctggaacg ccgcttgaaa gagttagaag
      601 gtctatctcc tgaagcacag agtatcttga gagaggcgca agcggctctt gctaatgact
      661 ccttaacagc tcaagaattg gcgaaactcc ttgctaaagt tagacaggct cttaattcta
      721 tacaagctgg cacatcagct gataagagtc cgtctacgtc aaactcaaat aaggaagcag
      781 aaagcgccaa ggaacctaca aatgcgacag atgtccctct atatggtgtc tttggagctg
      841 cagcactctc tcttctagga gctctcctct ttgcagtggc tcgtaagaaa aactctcaac
      901 tagataagct tttaagagaa ttggatcaac ttctagttga gttagaggct agtgacaagg
      961 ataaaaaagg tttaggcaag gctaaaaaat tagctaagaa agcaagaacc tttgtggata
     1021 gccagcaaaa agatcctcaa aaagaagcag aactaatttc tgaaatcaag actactctca
     1081 gtcaactcag agaaggagtc tagtcttgaa gaaaaaaact actttcaaaa atgcagtgag
     1141 tagttttgta gcgatgatga ggaaataaaa ggctctttgt caactgtagt gggttgaaaa
     1201 aaaactaagc tcgagaaagg acaaattttg tcctttcttt tttgatattc agagcgataa
     1261 aaatccgttt tttgaagttt tcaaagttcc gaaaaccaaa ggcattgcgc ttgataagtt
     1321 tgatgagatt attggtcact tccaatttgg cgtttgaata gggtagttga agggcgttga
     1381 cgattttctc tttgtccttt agaaaggttt taaagacagt ctgaaaaata ggatgaacct
     1441 gcttaagatt atcctcaata agtccgaaaa atttctctgg ttccttattc tgaaagtgaa
     1501 acagcaagag ttgatagagc tgatagtgat atttcaagtc ttgtgaatag ctcaaaagct
     1561 tgtctaaaat ctctttattg gttaaatgca tacgaaaagt aggacgataa aatcgcttat
     1621 cactcagttt aaggctatcc tgttgtatga gcttccagta gcgcttgata gccttgtatt
     1681 catgggattt tcgatgaaac tgattcatga tttgaacacg catacgactc atagcacggc
     1741 taagatgttg tacaatgtga aagcgatcca acacgatttt agcacacggg aaaagttgtc
     1801 tagctaagtc atagtaaggg ataaacatat ccatagtaat gattttgacg cgacatagga
     1861 cagctctatc gtagcgaaga aagtgatttc gaatgatagc ttgtgttctt ccctcaagaa
     1921 cagtgatgat attgagattg ttaaaatctt gcgcaatgaa gctcatcttt ccctttgtaa
     1981 aagcatactc atcccaagcc ataatctcag gaagacaaga aaaatcatgt ttaaagtgaa
     2041 aatcattgag cttacgaata acagttgaag ttgagatgga aagctgatgg gcaat
//
LOCUS       DAVKDZ010000150         2085 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000150 DAVKDZ010000000
VERSION     DAVKDZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.152"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(179..982)
                     /locus_tag="VWZ30_002167"
     CDS             complement(179..982)
                     /locus_tag="VWZ30_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEU9335809.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1261..2016)
                     /locus_tag="VWZ30_002168"
     CDS             complement(1261..2016)
                     /locus_tag="VWZ30_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEU9335810.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
BASE COUNT          679 a          415 c          317 g          674 t
ORIGIN      
        1 cgaattaagg taaaactaaa aggacttagc cctgtgcaat acagaactaa atccttcgga
       61 taaattaatt gtctaactgt ttggggtcag tacattttaa cacttgatac tatgcgtttt
      121 attgtgggaa tgtttacttc attttttcct aaaattcaat gtttactcag tatttggatt
      181 atgaaaaatc gaggtctaaa tctagataca ttttttctga agacaaatca ttttgaccac
      241 cgagcaagag attttcaaaa aaagctgtta aaaactcagg acgtcgctgt aaaatctttg
      301 cattatctaa taccaaggca tcacgaaaat acttggaatg ttgctgaaat ggtatattat
      361 caatatcaaa accaaactca cgaagatact gaatcaaaaa gaccgttact gtccgagtgt
      421 ttccttcgcg aaatggatga atctgccaga ttcctgaaat aaaatgctgg atttgtttaa
      481 ccacatccgc ctgagttagt gtcgcatatg caacttgttt ttcctgatta aaatcataat
      541 ctaaggtcat ttgaatcatg gagtaatcag agtacacaac actttcacca ttcaaaacag
      601 gttcattctt tgtgatattg gtctgacgaa attgccccac cggaatagag ggttcaaata
      661 tatcttgaaa caactcctta tgaatagcaa gtaaggtcgc aggactaaag ctaaagcctc
      721 ttcgagacaa tagttctaca atacgtagag aaaccaagtc tgcctcttcc gtacttgcat
      781 caatagtatg gtgataagcc gttgcatcct cataaacctg ctcataagtc agttctcccc
      841 gggactgttt ctcagccaaa gattccatat acgctgatgg cactagattg tcaactttct
      901 gcagaccaaa acctatccgc cataaatcac gcttcgcttc ataagacaag tttggattgt
      961 caatgttgta agttggttgc atagaaatat cctttcaatt gtttttcttc attaccagta
     1021 aatcacgaaa ttggttggaa actggcttaa actataaaaa gtcctcacaa taggttctat
     1081 gatatatata tatatacttc atgtaaataa taaaagagtt ataatcagca ctactgataa
     1141 attcaatacc gatttcgcca tcttaaaact tagtcaaacg aataccgttg atttccagtt
     1201 cattaagtcg ttgacctgtc aagacttctc gttccttcaa ctggaaaagt taagggggaa
     1261 ctaatacaaa tcctctacgc ttctatattc aatgatttca tttttgataa tattcttttt
     1321 aaactcaaaa tgttttaaag gattatcgat taacactaac tttggaatat catctagatt
     1381 tgtgaccaag gataaaataa aatccgattg aaactccttg actttttcaa attcttttgc
     1441 agttaaacgc acacgcccct tagattttcg gatacctttt acttcggcaa ggtaagaata
     1501 ctcttggaca tctacctgaa agtcatatcc atcaccataa agacgggcat cggttaactg
     1561 tccaccttgg aatttttctt ctttatcaaa atgaaggata aaataattct cagcctccat
     1621 tcccgtttcc tgtagacgtt tgaatttagt tcgaactatt ggtttttcaa cgatggttat
     1681 gcctgttttc ttcccttcac tggcaagtaa cattttaact atttcagcat aactatgaac
     1741 atcttcattt ccaaacatca tatcaattaa atctttacga aaacgataaa cttcagcttt
     1801 ctgccaccaa ccttttcgat tattgtcaaa ataaggatca aataaatcca ttctattttt
     1861 tacaactcca gttgtttcag caattcccag agaaaccaca tagcgataaa actccgactt
     1921 gctagagcat tgaaattctt ttataaaaag cttgtcaaac ttggcaagac cgtagccaat
     1981 taaatttaac aattcataat gagggtgttt ggacatcact ttctccatca atagatattg
     2041 atttcattat accaaaaaac acggcattac accatgtttc tatgt
//
LOCUS       DAVKDZ010000151         2065 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000151 DAVKDZ010000000
VERSION     DAVKDZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.153"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            207..1679
                     /locus_tag="VWZ30_002169"
     CDS             207..1679
                     /locus_tag="VWZ30_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9335811.1"
                     /translation="MRDLLSKKSHRQLELLFEHKRWFHRSELAELLNCTERAVKDDLS
                     HVKSAFPDLIFHSSTNGIRISNTDDSDIEMVYHHFFKHSTHFSILEFIFFSEGCQAES
                     ICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQVIGNERDIRYFFAQYFSEKYY
                     FLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFMEVDK
                     DSFNDQSLNALMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDGSLFMK
                     CVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELSTEFILF
                     DQKGNTIRNFQNIFPKFVSDVKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHLVIN
                     LLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSPYDI
                     IISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
BASE COUNT          719 a          297 c          324 g          725 t
ORIGIN      
        1 aatataatct ttagacttga atttgattct gtattcacgc cctcaataga cggtaaaata
       61 agaaaattgt ttatatattg tctccgtagt gttattatac gaaataaaag attttaggaa
      121 aagtcgttat atcatgctat acctattctt tgtggtataa ttgcaagagg tttaatccga
      181 taatttataa agagagaaag atattcatga gagatttatt atctaaaaaa agtcataggc
      241 aattagaatt attatttgaa cataaacgtt ggtttcatcg ttctgaacta gcagagttat
      301 taaattgtac agaacgtgca gtcaaagatg atctatccca tgttaaatct gcttttcctg
      361 acttgatttt tcattcttct actaatggta tacgcattag taataccgat gatagtgata
      421 ttgaaatggt ttaccatcat ttctttaaac attcaactca tttttcgatt ttagaattca
      481 tcttctttag tgaaggttgt caagctgaaa gtatttgtaa agaattttat atcagttcat
      541 cttcgctcta tcgtattatt agccaaatca ataaagtgat taaaaggcaa tttcaatttg
      601 aagtcagtct gacccctgtt caagtcattg gaaatgagag agacattcgt tacttttttg
      661 cacaatattt ttcagaaaaa tattatttcc tagaatggcc atttgaaaat ttttcatcag
      721 agccactatc tcaattgtta gaattggttt ataaggaaac aagctttcca atgaatttgt
      781 caacgcatag aatgctaaag ttgctcctag ttacgaacct atatagaata aagtttggtc
      841 attttatgga agtagataaa gattctttta acgaccaaag tttgaatgct ttgatgcagg
      901 cagaaggaat agaaggtgtt gctcagagtt ttgaatcaga atacaatatc tctttagatg
      961 aagaagttgt ttgccaatta tttgtgtctt actttcaaaa aatgtttttc atagatggaa
     1021 gtctctttat gaaatgcgta aaaaaggata gttatgttga aaaatcttac catctattga
     1081 gtgattttat tgatcagatt tcagtcaagt atcagattga aatggagaat aaggataatc
     1141 tgatttggca tctgcataat accgcacatc tgtatcgtca agagttgtcc actgagttta
     1201 ttttatttga tcaaaaaggg aatacaatca ggaactttca aaatattttt ccaaaatttg
     1261 tttcagatgt aaaaaaagag ctttctcact atttagagac tcttgaggtt tgttctagtt
     1321 caatgatggt aaatcatcta tcttatacct tcatcactca taccaaacat ttggtgatta
     1381 atttgctaca aaatcagcct aaactaaagg ttttggttat gagtaatttt gatcagtatc
     1441 atgcaaaatt cgttgcagag acactttctt attactgtag caataatttt gaactagaag
     1501 tttggactga attagaatta tcaaaggaat ctttagaaga ttcaccttat gatatcatta
     1561 tttctaattt tattattcct cctattgaaa ataagagact catctattca aataatataa
     1621 acacggtctc actcatatat ttgttaaatg ccatgatgtt tattcgatta gatgagtaac
     1681 caaaaataac aaaaaaacgc tatttcgcgt ttgaactgta cctcaaaagt tagacagaaa
     1741 aaatctaatt tttggggtgt ttttgttaag aaaagacctt caatcattta agcaaaatgt
     1801 acatctagat ttatagcctt ctaaaactct tacttttttt gtagaagata cgaaaaaagc
     1861 catgaaacca ggctctataa tatttgtagt gggtaaatcc cctatggata ttatggagcc
     1921 tattttaggg tagaaaaaaa gtcccatatc atctataatg aaaagagaca aaataactca
     1981 ttagaaagaa tcatatggaa caattacatt ttatcacaaa actactcgat atcaaagacc
     2041 ctaatatcca atttatggat atcat
//
LOCUS       DAVKDZ010000152         2031 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000152 DAVKDZ010000000
VERSION     DAVKDZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.154"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..1835
                     /locus_tag="VWZ30_002170"
     CDS             <1..1835
                     /locus_tag="VWZ30_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9335812.1"
                     /translation="DLSASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLT
                     VTPTYHQNQGENISSLLRELYAKPLSERHVESDGLVFDPAQITYRTANGVAVPHGDHY
                     HFIPYSQMSPLEEKLARMIAIKGQNGAVLPGMHYLKPAPKPQVQPSTEKKQTDFAVEQ
                     VVRKVGEGYVVEIAGVSHYVFAKDLAKDKIDAIENHLSKKTQETHALVAKKESVASRD
                     REFYDKAYNLLTQAHKALSENKGRTSDFHALDKLAERLNNESSNKVKLVDDLLTFLAP
                     ITHPERLGKPNAQIAYTDDEIKLAKLAGKYTTEDGYIFDEHDIESDEGNAYVTPHLNH
                     SHWIKKDSLSEAERAAAQAYAKEKGLQAPNSTEATEAKTEATGTEAIYNRVKAEKKVP
                     LEKIPNHLQYTVAVQNGHFIIPHFDHYHNLKFAWFDEGLYSAPEGYTLEDLFATVKYY
                     VEHPDQRPHSDNGWGNASDHVRKHQADQDSKPDENKEHEEVSEPTRPESDEEENHAGL
                     NPSADNLYKPSTDTEETEEEAEDTTEEAEVPQVERSIIEVKITEAEALLAKVTDSRIQ
                     QNAMETLAGLQSSLLLGTKDNNTILAEVDSLLALLKESKPTPIQ"
BASE COUNT          715 a          372 c          407 g          537 t
ORIGIN      
        1 gtgatttgtc tgccagtgaa ttagctgctg cccaagccta ttggaatggg aagcagggat
       61 ctcgtccttc ttcaagttct agtcataatg caaatccagc tcaaccaaga ttgtcagaga
      121 accacaatct gactgtcact ccaacttatc atcaaaatca aggggaaaac atttcaagcc
      181 ttttacgtga attgtatgct aaacccttat cagaacgcca tgtagaatct gatggcctag
      241 tatttgatcc ggcacaaata acatatcgta cagccaatgg tgttgctgta ccgcacggag
      301 accattatca ctttatccct tattcacaaa tgtcaccttt ggaagaaaaa ttggctcgta
      361 tgatagctat caagggacaa aatggtgcag tcttgccagg aatgcattat ttgaaaccag
      421 caccaaaacc gcaagttcaa ccatcaactg agaaaaaaca aactgatttt gcagtagaac
      481 aggttgttag aaaagttgga gaaggctatg ttgtggaaat tgctggtgtc agtcactatg
      541 tttttgctaa agacttggct aaggataaga ttgatgctat tgaaaatcac ttgtctaaga
      601 aaactcaaga gacacatgct ctagttgcta aaaaagaaag tgtcgcttct cgtgaccgag
      661 aattttatga taaagcttac aatctgttaa cccaggctca taaagccttg tctgaaaata
      721 aggggcgtac ttctgatttc catgccttag acaaattagc agaacgcttg aataatgaat
      781 cttctaataa agtaaaattg gtagatgatt tattgacatt cctagcacca attactcatc
      841 cagagcgact tggcaaacca aatgctcaga ttgcttatac agacgatgaa attaaattag
      901 cgaaattagc aggtaaatat acaacagagg atggctatat ctttgatgag catgatattg
      961 aaagtgatga aggaaatgcc tacgtcactc cacacttgaa ccatagccac tggattaaaa
     1021 aagacagctt atctgaagct gaaagagcag cagctcaggc ttatgctaaa gaaaaaggct
     1081 tacaagctcc aaattctact gaagcaacag aagcaaaaac agaggcaaca gggacagaag
     1141 ctatctataa ccgcgtaaaa gctgagaaaa aggtaccatt agaaaaaata ccgaatcatt
     1201 tacagtatac tgtagctgta caaaatggtc actttataat tcctcacttt gatcactatc
     1261 ataaccttaa gtttgcatgg tttgatgaag ggctttactc agctcctgaa gggtatactt
     1321 tagaagattt atttgcgaca gtgaaatact atgttgaaca tcctgaccaa cgtcctcact
     1381 cagataatgg ttggggtaac gctagcgacc atgttcgtaa gcatcaggca gaccaagaca
     1441 gtaaacctga tgaaaataag gaacatgagg aagtaagtga gccaactcgc cctgaatctg
     1501 atgaagaaga gaatcacgca ggtttaaatc cttcagcaga taatctttat aaaccaagca
     1561 ctgatacgga agagacagag gaagaagctg aagatactac agaagaagct gaagttcctc
     1621 aagtagagcg ttctattatt gaagttaaga taacagaggc ggaggcttta cttgcgaaag
     1681 taacagattc tcgtattcaa caaaatgcta tggagacatt ggctggtcta caaagcagtc
     1741 ttcttctcgg aacgaaagac aataacacta ttttagcaga agtagatagt ctcttggctt
     1801 tgttaaaaga aagtaaaccg actcctatac agtagtaaaa tgaatggagc atattttatg
     1861 gagaagtaac ctttcgtgtt gcttctcttt tttagaaaaa cgtaacagaa acttgacaaa
     1921 agtaatttta aatagtaaac catttactgg ttaaataacc ggtttagaaa actatttaat
     1981 caagtaaaag aagttgagaa aaagcttcat catttattga aatgagggat t
//
LOCUS       DAVKDZ010000153         1921 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000153 DAVKDZ010000000
VERSION     DAVKDZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.155"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            112..1845
                     /locus_tag="VWZ30_002171"
     CDS             112..1845
                     /locus_tag="VWZ30_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEU9335813.1"
                     /translation="MRVKQSKSKNTINYAIIKDIKVGNKRTSTIVENLGNHNTLLKEH
                     PDMEPLEWARLRAKELTEKEKEENKDFLITFSQNKQLKQNQLNEYHGGYLFLQDLYHQ
                     LDLPRINKEIQKRHRFNFSLDDILSRLIYGRILAPASKRSTLEFSKTLLEPKEFELQH
                     FYRALEVISNETEFIQEQTYQTSKKMTQRQTEVLYYDCTNFYFEIEAEDEEGLLRQYG
                     PSKEHRPNPIVEMGLFMDKQGIPLAFCIHPGNTNEQTTLKPLERKIVKEYKASKFVVC
                     TDAGLSSKANKMYNSFGERSYVTTQSIKKLKKEYRDWALDKSGWRMVGDKNPIFYNLD
                     ELEHREELKKQLFFKESPFYDEDLPNQRLIVTFSTKYQDYHRTIRERQIERASKLLGK
                     PSQATKKRQTDFKRFLKETSTTKEGEIAEKRILTLDEERIQEEAQYDGLYAVATNLDE
                     DIQTIVVINQRRWEIEESFRIMKQELRARPVYLSRSDRIQAHFTICFLALMIYRLLEK
                     QLGETVTCSELIQTLRNYKFKHLYGAGYLPTYTRTTITDQLHQAFGFQTDFEIISEKN
                     MKKIFKKTKSR"
BASE COUNT          704 a          362 c          341 g          514 t
ORIGIN      
        1 gtgaaaaact cctcataaag gttgatataa ggggattttc ttgtcaaatt tcaccgtttt
       61 ttatttgcgt acttttccgt actttgttat aatagagcga ggtataaaaa gatgagagtg
      121 aaacaatcaa agtcaaaaaa cactatcaac tacgctatta ttaaagacat caaggttgga
      181 aataaacgga cttctactat cgtagaaaac ttaggcaatc ataacacact cttaaaagaa
      241 catcctgata tggaacctct tgagtgggct cgtttacgtg ccaaggaatt aactgagaaa
      301 gaaaaagaag aaaataaaga tttcctcatt acctttagtc agaacaaaca gttaaaacaa
      361 aatcaattga acgaatatca tggaggctat ctcttcctac aagacctcta tcatcaacta
      421 gatttgccta gaattaacaa agaaatccaa aaacgacatc gttttaattt ctctctagat
      481 gatatcctat ctcgattgat ttatggacgg attctagctc ctgcttcaaa acgatccacc
      541 ctagaatttt caaaaacatt actcgaacct aaagaatttg aactccaaca tttttatcgg
      601 gctcttgaag ttatttctaa tgaaacagag tttatacaag aacaaaccta tcaaacttca
      661 aaaaagatga cccaacgaca gacagaagtt ctctactatg actgtactaa cttctatttt
      721 gaaattgaag ctgaagatga ggaaggactt ctgagacaat acggtccatc taaagaacat
      781 cgcccaaatc caattgtgga gatgggactc tttatggaca aacaaggaat tcccctagct
      841 ttctgtatcc accctggaaa tacaaatgaa caaactactc ttaaaccatt agaaagaaag
      901 attgtcaagg agtacaaggc ttcaaaattt gtagtctgta cagatgctgg actctcatca
      961 aaagccaata agatgtacaa ttcttttggg gaacgttcct atgtcacaac tcaatctatc
     1021 aagaagttaa agaaagaata cagagactgg gctttggata aaagtggttg gcgaatggtt
     1081 ggggataaga atcctatctt ttacaatctg gatgagctag agcacagaga ggaattaaaa
     1141 aaacagctct tctttaaaga atctcctttt tatgatgaag atttaccaaa tcaacgcttg
     1201 attgtgacct tttctactaa atatcaagac tatcatcgaa cgatacgaga gagacagatt
     1261 gaacgggcta gtaagttact gggcaagccc agccaagcta ccaagaagag acaaaccgat
     1321 tttaagagat ttctgaaaga aacaagtact accaaagaag gggaaatcgc cgagaaaagg
     1381 attctaactc ttgatgagga acggattcaa gaagaggctc aatatgatgg tctctacgcc
     1441 gtagccacta atctagatga agatatccag acaattgtag tcatcaatca aaggcggtgg
     1501 gaaattgaag agagttttcg tatcatgaag caggaactac gagccagacc cgtttatctc
     1561 agccgttcag ataggattca agcacatttc actatttgtt tcctagcact aatgatttat
     1621 cgtctcttgg aaaaacaact aggagaaaca gtcacctgtt cagaactcat ccaaacctta
     1681 cgcaattaca agttcaaaca tctttatgga gcaggatatc ttccaactta caccagaaca
     1741 actatcacgg atcaactcca ccaagcattt ggtttccaaa ctgattttga gattataagc
     1801 gaaaaaaata tgaaaaaaat ttttaagaaa accaaatcta gataaaaagt acgcattttt
     1861 taaaaagaga aaaagagctc cgaaaagctg tcatatcagt atttaggggc tctttgattt
     1921 c
//
LOCUS       DAVKDZ010000154         1916 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000154 DAVKDZ010000000
VERSION     DAVKDZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.156"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            1..471
                     /locus_tag="VWZ30_002172"
                     /pseudo
     CDS             1..471
                     /locus_tag="VWZ30_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375503.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="lanthionine synthetase"
     gene            471..1697
                     /locus_tag="VWZ30_002173"
     CDS             471..1697
                     /locus_tag="VWZ30_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9335814.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFPLL
                     LVAFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVTQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
BASE COUNT          546 a          323 c          339 g          708 t
ORIGIN      
        1 aaatagagga gttatattta tattttgtag atgagatacg aaatgttaat agtttaaaag
       61 aatgtaattt tgcacatggt ctagctggaa ttgcatacgt aattttaatt tataattcgg
      121 aatttgaatt caaggtagct tacgaaaata gtattaaggt attcaataat ctgttagaaa
      181 atttgttaga aaatgaatat agtagactaa atgcaactcc tacaaattta gaactatcgt
      241 ggtgcgaagg aacttcagga atcttacttt attttgagtt attatatgac aatacgtgag
      301 aatgtgattg agattttatt aagtagatcc aaaagagata aaaatggtat tttggtattt
      361 gaatgtgaag aaagttcaga tacattattt gattttggta tcggaaatct agggatttat
      421 tggtctatcc ttggggaaag attcccattt gaacttaaga aggagttgtg atgaaagtat
      481 ttcttcaaaa tagagatttt aggcaattaa ctatcaacca gtggatttca acgcttgggg
      541 atacgatttt ttatctggcc ttcctgaatt atgtggcaga tgcatctttt gcccctttgg
      601 cgattttact catcacgatt tcagaaaccc tgccccaagt tctacaaatc tttctgggag
      661 ttttggcgga ttttcaacat catcgtgtct tgaaatacac agtcattagt tttgcaaaat
      721 ttttgcttta ctctatagtg tctttatcac tttcagggca gtcctttccc ttgttattag
      781 tagcatttat ttgtctgatt aacctcttgt ctgacacgtt gagttatttt tcaggcgcca
      841 tgctcactcc gattttcatt agaattattg ggcaagacca tctggcagaa gctattggat
      901 ttaaacagtc aactgttagt ttagtgaaaa caatcagtaa tattctagga ggagtcttat
      961 taggtattct atccatccag tttatttcct tgctgaacgc tcttaccttt ttaatcgcat
     1021 ttttaggtat cctcttcata aaaactgatc tcttgaaagt agaaaaaacg attaactatc
     1081 aagaaggact ctctgtaaaa tccttttgcc agcatttgct ccaatcatcg aaattgatat
     1141 ggaatatgaa taaggtgctc ttggttttgt ttattatctc tactagtcaa gcagtgataa
     1201 atgttacagt tcctgtctct actctatttt tgaggaatca accctttttg aatttacaaa
     1261 caggtcaatc tcttgccttg ctatccactt ttgaactgtc agccctgatt gtcggaagcc
     1321 ttgtaagtgg ctatctgcaa cataccattt ccataaaaac agctctctat gcctcacttg
     1381 tcacccagtt gcttcttcta gtaggatttg ccacagttcg ttttgactgg attctcatct
     1441 ttagtacctt ggatgccttt ttcgcaggtg ttctctctcc tagattgcaa gaactcgttt
     1501 ttaaacaaat acctgaggag tcaatgggag cagttcaatc ctctatcggt gccattacgg
     1561 ttgttttacc tagcttattt acaatagctt tggtaaccat tgctactagc tttggaactc
     1621 tggcagttag ctttgtttta ttgctatttc ttctagttgc ctttgttatg cttctgaata
     1681 ttcgtgaaag tatttagtgg agaatttcta taatatatgt ttgagactgg attgccagcc
     1741 tttttatgat ggtcaaaatc aacagacttt tttctctttt ctgaatgtgt tataatagac
     1801 tgaatctgaa atagtacgaa acaattgcta aaacatttat agaaattaat tttactttcc
     1861 caatcgattt gttctcatct tatttcaatc tgctatagtc aattgaaaca agagca
//
LOCUS       DAVKDZ010000155         1817 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000155 DAVKDZ010000000
VERSION     DAVKDZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.157"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            1..1732
                     /locus_tag="VWZ30_002174"
                     /pseudo
     CDS             1..1732
                     /locus_tag="VWZ30_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399232.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT          688 a          284 c          346 g          499 t
ORIGIN      
        1 cgaatcaaat tggacaaccg actcttccaa ataatggtct aacaacacct tcgccatctc
       61 ttccagtcaa tcctgatgtt tcacatgagg aacatgaaga agatgggcac ggctttgatg
      121 ctaatcgtat tattgctgaa gatgaatcag gatttatcat gagtcacggt aatcataatc
      181 attacttctt caagaaggac ttgacagctg atcaaattaa ggctgcgcag gatcacttga
      241 aaggtggaat gcttgctaca ccaactccag cctatgatga tcacgaccac catcacggtg
      301 agggacatga ttccagcttt gatgctaatc gtattatcgc tgaagatgaa tcaggattta
      361 tcatgagtca cggtaatcat aatcattact tcttcaagaa ggacttgaca gctgatcaaa
      421 ttaaggctgc tcaaaaacat ttagaggaag ttaaaactag tcataatgga ttagattctt
      481 tgcaatctca tgaacaggat tattcaggta atgccaaaga aatgaaagat ttagataaaa
      541 aaatcgaaga aaaaattgct ggcattatga aacaatatgg tgtcaaacgt gaaagtattg
      601 tcgtgaataa agaaaaaaat gcgattattt atccgcatgg agatcaccat catgcagatc
      661 cgattgatga acataaaccg gttggaattg gtcattctca cagtaactat gaactgttta
      721 aacccgaaga aggagttgct aaaaaagaag ggaataaagt ttatactgga gaagaattaa
      781 cgaatgttgt taatttgtta aaaaacagta cgtttaataa tcaaaacttt actctagcca
      841 atggtcaaaa acgcgtttct tttagttttc ctcctgaatt ggagaaaaaa ttaggtatca
      901 atatgctagt aaaattaata acaccagatg gaaaagtatt ggagaaagta tctggtaaag
      961 tatttggaga aggagtaggg aatattgcaa actttgaatt agatcaacct tatttaccag
     1021 gacaaacatt taagtatact atcgcttcaa aagattatcc aaaagtaagt tatgatggta
     1081 catttacagt tccaacctct ttagcttaca aaatggccag tcaaacgatt ttctatcctt
     1141 tccatgcagg ggatacttat ttaagagtga accctcaatt tacagtgcct aaaggaactg
     1201 atgctttagt cagagtgttt gatgaatttc atggaaatgc ttatttagaa aataactata
     1261 aagttggtgg aatcaattta ccgattccga aattaaacca aggaacaacc aaacggccgg
     1321 aaataaaatt cctgtaacct tcatggcaaa tgcttatttg gacaatcaat caacttatat
     1381 tgtggaagta cctatcttgg aaaaagaaaa tcaaactgat aaaccaagta ttctaccaca
     1441 atttaaaagg aataaagcac aagaaaactc aaaacttgat gaaaaggtag aagaaccaaa
     1501 gactagtgag aaggtagaaa aagaaaaact tactgaaact aggaatagta ctagtaattc
     1561 aacgttagag gaagctccag cagtagatcc tgtacaagaa aaagtagaaa aatttacagc
     1621 tagttacgga ttaggcttag atagtgttat attcaatatg gatggaacga ttgaattaag
     1681 attgccaagt ggagaagtga taaaaaagaa tttatctgat ctcatagcgt aaggaatagc
     1741 agtagaaaaa gtctgaatca aaaatgaagt tctttcaaaa gttagaaata aaactctgac
     1801 tttgggagaa tttcatt
//
LOCUS       DAVKDZ010000156         1777 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000156 DAVKDZ010000000
VERSION     DAVKDZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.158"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(<1..215)
                     /locus_tag="VWZ30_002175"
     CDS             complement(<1..215)
                     /locus_tag="VWZ30_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU9335815.1"
                     /translation="MWMEELPNGKYKFFERYKDPYTEKLKKVSVTMEKKTPQARNQAA
                     ILLQEKINKKLSTKQVESITFEEIYNL"
     gene            complement(332..847)
                     /locus_tag="VWZ30_002176"
     CDS             complement(332..847)
                     /locus_tag="VWZ30_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335816.1"
                     /translation="MKRIFSISSVLLCALVISTACTNQTQKSSSEPTTQASSSDDISV
                     EEYFDKLLTKVDKVTKDNYKSDDYKIYDYKTVLREPEKFFSLKMRIDNLEIVQIFKEG
                     KYTKMLVTQPSGDFYMLFMETKRIEKRFLEKDHLTVNGRYLLSYEYTTNSNKEKSVPL
                     IYVDAYLLLKN"
     gene            complement(865..1245)
                     /locus_tag="VWZ30_002177"
     CDS             complement(865..1245)
                     /locus_tag="VWZ30_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEU9335817.1"
                     /translation="MTEKEFSQNLGIDIEIFEDGLFPDEAFYIPALKTMFLSDAISDE
                     KRVQVALHEIGHRNHAPDTYQLFREKCELEANRNMIHHLMKAELGIAEDATTFNYLVF
                     MEKYNLKTIADEIMVKEEYLALLN"
     gene            complement(1267..1629)
                     /locus_tag="VWZ30_002178"
     CDS             complement(1267..1629)
                     /locus_tag="VWZ30_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9335818.1"
                     /translation="MFETFEKIKSLAKKQGISLNTLEDRVGLGKNYIYSLKNKKTPSA
                     EHISKIADYFNVSTDYLLGRTDNPTIANKKEQFFFEGKEVDVEELASTAMRFNGKPLT
                     EEDKKAIQNIIEIYLRKQ"
BASE COUNT          522 a          307 c          281 g          667 t
ORIGIN      
        1 aaaaggttat agatttcttc aaatgtaatg ctttctactt gttttgtgct gagtttttta
       61 tttatcttct cttgtaacaa gatagcagct tgatttcttg cctggggagt tttcttctcc
      121 atggtcactg aaactttttt taatttctca gtatatggat ctttatatcg ctcaaaaaat
      181 ttgtattttc cgttgggaag ttcttccatc cacattgcgt ttacctcact tttttgataa
      241 aatgggtata gtaaagaggg ctttttaatg ccttttacta tacaggatat cctcacactc
      301 tccttggcca aaatttgagt gtggggattt tttaattttt cagcagcaaa tacgcatcga
      361 cgtaaattaa aggtacgctt ttctctttat tactatttgt agtgtattcg taagaaagca
      421 agtaacgccc gtttacagtc aaatgatctt tttctaaaaa tcttttttcg atacgtttag
      481 tctccatgaa cagcatatag aaatcaccgc tgggttgtgt aacaagcatt ttcgtgtact
      541 tgccttcttt aaaaatttgt acgatctcca gattgtcgat tctcattttt aaagagaaga
      601 atttttctgg ctctctgaga actgttttgt aatcgtagat tttataatcg tctgacttgt
      661 aattgtcctt tgtgacctta tcaactttag tcaatagctt atcaaaatat tcttctacgc
      721 taatatcatc agatgatgat gcctgagtgg tcggttcgct ggatgacttt tgcgtttgat
      781 ttgtacaagc ggttgaaata acaagtgcac aaagcaaaac agatgatatg gaaaatattc
      841 ttttcatttt atcctactcc tttttcaatt aagtaaagcc aaatattctt cttttaccat
      901 tatttcatcg gcaatggttt ttaaattata cttttccata aataccaggt aattaaatgt
      961 agtggcatct tcggctatac ccaactcagc tttcataagg tgatggatca tattcctatt
     1021 agcctcaagc tcacactttt cccgaaaaag ctgataagta tctggcgcgt ggtttctatg
     1081 gcctatctca tgtaaagcga cttgtaccct tttttcatca gatatagcat cactcaaaaa
     1141 catagttttg agggctggga tgtaaaaggc ttcatctgga aatagaccat cttcaaaaat
     1201 ctctatatct atgcctagat tttgagaaaa ttctttttca gtcataaaca atccaacctt
     1261 tgattattat tgttttctga gatagatctc tattatgttt tgaattgctt ttttatcttc
     1321 ttcagttagt ggtttaccat tgaagcgcat agcagtagag gcaagttcct caacatcgac
     1381 ttctttgcct tcgaaaaaga attgttcttt tttattagcg atggtaggat tatctgtgcg
     1441 accaagtaag tagtctgtag atacattgaa gtagtcagcg attttggata tatgttcagc
     1501 agatggagtc tttttatttt taagactgta aatgtaattt ttccccaaac caaccctatc
     1561 ttctaaagtg tttagagaaa ttccctgttt tttagctaaa cttttaattt tttcgaatgt
     1621 ctcaaacatt gatacatcaa cctttctgaa gcattacaaa aaaatatttt attttttcgc
     1681 ctaaaacact tgacaaaaat tagacgaaca actaaaatag tatttgtaag taattaacaa
     1741 ctaaaaaaac aactaagaaa taaattataa aaatgtt
//
LOCUS       DAVKDZ010000157         1770 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000157 DAVKDZ010000000
VERSION     DAVKDZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.159"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(<1..589)
                     /locus_tag="VWZ30_002179"
     CDS             complement(<1..589)
                     /locus_tag="VWZ30_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9335819.1"
                     /translation="MKIENESKSYDKCILKNISLEIDKGIYRVEGENGSGKSTLLQLL
                     AGLETFDSGLKNCVSKDVLYLDTNQLGVVPFTIEENLQLLCDSFQIHLSFEDKEQINT
                     FFDNKIGDNYMTASTGTKFKLGLSLIFAKNWDYIFIDETLSTVDARSIDMIAKRLISM
                     KESSTIIYVSHNLLNQELISKSQRILIEEGRVYEIS"
     gene            complement(586..1068)
                     /locus_tag="VWZ30_002180"
     CDS             complement(586..1068)
                     /locus_tag="VWZ30_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEU9335820.1"
                     /translation="MRKSGFLIYYVLFCIVCFLLGLLLSFILKNETFATIPPKLELQF
                     TLTVLQANSTNFLLYLFCFPISPLLQLFDLLSLGIQLGISGQMIGIVATIKGILPHAL
                     IEIPNFIWYQGMSQYLLYRLLKDRGIKNILIYIKSNLKWYIISYLLLILAALIEGIKL
                     "
BASE COUNT          700 a          288 c          205 g          577 t
ORIGIN      
        1 ttgaaatttc ataaacccgt ccttcctcta ttaaaatcct ttgagatttg ctaatcaatt
       61 cttgattcaa taaattatga gatacatata taatagtaga gctttctttc atggaaatta
      121 agcgtttagc aatcatatca atacttcttg catccactgt tgataaagtc tcatcaataa
      181 aaatataatc ccaattttta gcaaaaatta aggataagcc taatttgaat tttgttccag
      241 tagatgctgt catatagtta tctccgattt tattatcaaa aaaagtatta atttgttctt
      301 tatcctcaaa tgatagatgg atttgaaaag aatcacacaa caactgcaaa ttttcttcta
      361 tagtaaatgg aacaacccca agctgattgg tatcaaggta tagtacatct ttggagacac
      421 aattctttaa accactatca aaagtctcta gcccagctaa aagttgtaga agggtgctct
      481 ttcctgaacc attctctcct tcaactctgt aaattccctt atcaatctct agtgatatat
      541 ttttcaatat acatttatca taacttttag attcattttc aattttcata atttaattcc
      601 ctctatcaac gctgctaaaa ttaataaaag ataagaaata atataccatt ttaaattact
      661 ttttatataa ataagtatat ttttaattcc cctatccttc aataatcggt ataaaagata
      721 ctgactcata ccttgatacc aaataaaatt cggtatttca ataagagcat gaggtaatat
      781 tccttttatt gtagctacta ttccaatcat ctgaccagat atacccaatt gtattcctaa
      841 agatagcaaa tcaaaaagtt gcaataaggg cgaaatcgga aaacagaata gatatagcaa
      901 aaaatttgta gaatttgcct gtaaaactgt caatgtaaat tgtaattcca gtttcggagg
      961 tatagtagca aaagtctcat tttttaatat aaagcttaaa agtaaaccta gtaaaaaaca
     1021 gactatacaa aataacacat agtaaatcag aaaaccactt tttctcatct aatgccccct
     1081 actaaaatta caaatattat cagaaaacaa tataaaacca gaaaactcca tctcaataaa
     1141 ctaactgtac ctttaattaa aaatagagct ttcttataat agacataggc tataaaataa
     1201 ttgaatggaa tcaataacaa attcaacaaa atacttttta tatcattact ataacttgtc
     1261 accggaatca aacataacaa caatgaaaat tgtaagaaag agattaaatg cgtcaacatc
     1321 attagttgtg cattcttaaa aaagaaattc attttagaat gattaagagc agataattga
     1381 agtaaagcta gatctacccc aattgaggat ttatgattcc ctattattgc ctccattaat
     1441 gtaatatata ttgaggtata aagaaataat attggtattt ttgtgtcatc aattgataac
     1501 atcgtcatca tataccaaaa tagataggaa agaagaatac taatcaaaat aaaagaaaaa
     1561 gttaaccctt tatttaacca acaaaaacga agcaatataa atagactgct acgaaatctt
     1621 ttttgactca ttcgactgct tttaaaatag aaattactcg catgattatc taatatcccc
     1681 atgactaaaa taaaataatt gagtagtatg acataaataa caacttgtcc ttgttctata
     1741 gacaatagtc ctatagataa aaataagaac
//
LOCUS       DAVKDZ010000158         1739 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000158 DAVKDZ010000000
VERSION     DAVKDZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.160"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(28..813)
                     /locus_tag="VWZ30_002181"
     CDS             complement(28..813)
                     /locus_tag="VWZ30_002181"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU9335821.1"
                     /translation="MKHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVNEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRGSSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            complement(873..995)
                     /locus_tag="VWZ30_002182"
     CDS             complement(873..995)
                     /locus_tag="VWZ30_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase"
                     /protein_id="HEU9335822.1"
                     /translation="MKIKEQTRKLAADCSKHCFEVVDETDEVSSKYCFEVADGS"
     gene            complement(1163..1591)
                     /locus_tag="VWZ30_002183"
                     /pseudo
     CDS             complement(1163..1591)
                     /locus_tag="VWZ30_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
BASE COUNT          494 a          409 c          285 g          551 t
ORIGIN      
        1 aaattcaaag aatgatgcaa gtatttttta ttttcctgta aatttaggtc ttcttctctc
       61 cgaatgagct cgcactcctt ctttgaaatc ctctgtttga gctaaggatt tctgtaggtt
      121 cagttctaaa gtagcgtaac cctgccagtc tttaaattgg ctctcccaaa ccaacttctt
      181 aatggcagca taggaattac ttgaaccacg tcttaatttt ttaagaagct gttctctcgt
      241 cttttcaagt ttttcagctt cagaaacgcg gtaaaccaac ccccattcta gggctttttc
      301 tgccgttaaa gcctctcctg tcatagctaa ttgagcagca cgcgtcacac cgatactgcg
      361 gcttaagaga tgaattcccc ctgcatctgg agccaaacca acaccaacaa aggcttggat
      421 aaacttagcc ttatccgtcg ccaaacagaa atctgccgca acagccatat tcgctgcggc
      481 acctgcaaca gcaccgtcaa cttccattaa aacaggtttg gctatttgct tgattttata
      541 agaaatcgta ttgaccaact ctgcgatttt tgtcagtgat ggaatatcat cctcattcac
      601 tgcccgcttc atctctacca aatctccccc aactgagaag acctttccat tggcattgat
      661 taagataaaa tgcacagctg gattttcttc tgccaaagtc agagcttcta aaatctcctc
      721 acacatggga atatgaaaac catttgcgac ctcaggacgg ttcaaggtaa ggattgccaa
      781 gtcctcttca agctgataaa taatgtgttt catcagagct cctttttatt ttataagcag
      841 tttatactct tcgaaaattt cttcaaaccg cgtcagcttc catctgcaac ctcaaaacag
      901 tattttgagc tgacttcgtc agtttcatct acaacctcaa agcagtgctt tgagcagtct
      961 gcggctagct tcctagtttg ctctttgatt ttcattgagt gttaaattat ttcactttca
     1021 aagtatatct tttttaggag cagaaagcaa gaaaaaatcg actgaaagtg tctcagccga
     1081 taagaaaggc tcgctttagg ctagaaagca tcttattgct ctggcacata atgagcaacc
     1141 tggcttgttt ttgcaaaagc cattatttca aatcctccaa aaattgataa agattggtca
     1201 aacctttgct catgacatcc atttcttcct tactcacacc atcaatgatt ttttcaacca
     1261 tggccttgtg gaagcgttta tgtagcctat gaaccaagcg acccttcttt gtcaaatgta
     1321 gatgcaccac acgacgatcc tgctctgagc gaatgcgttc aatgtaagcc ttgcgttcga
     1381 gattattcaa actagtcgta actgtcccaa gaatcaccag caactctttt gacacttgac
     1441 ttggtgtcac gtctgggaat ttcccaatca catcaatcgt atgcatttct ttgatggaga
     1501 tatccttgaa acgactacct ctcaagttca cttcctcaat gacaaggaca ttgttaaata
     1561 ttcattaatt cgttggtagt ccatttttca tatccctcct tcttcaaaaa aacttttaca
     1621 atcaaaacat ttgacaagag aattatatca aagctcaaac aatgatgcaa gtatttttta
     1681 ttttcctgta aatttaggcc ttcttcgttt gaaaacgctt atatatactc ctaaggaaa
//
LOCUS       DAVKDZ010000159         1724 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000159 DAVKDZ010000000
VERSION     DAVKDZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.161"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            270..1061
                     /locus_tag="VWZ30_002184"
     CDS             270..1061
                     /locus_tag="VWZ30_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEU9335823.1"
                     /translation="MYQKLLRKIAEEKPSYNQEEIQWLLDHLGDPSPEIRDDLVFTSF
                     ARGIQEELFTQEQFYFIAEGISSDGGLDKDIDKVGLSTLERSFRALIYVNLLSADANQ
                     QSIFYQRLKSEIRNVLLNQGLYYLSKEKDTRGFSSQYGWVHAFAHGADLLTEVVCHPN
                     FPKTRVHEVFDILGQLFKRITIRFTDDEDWRLARVLYEPILQGKLEQEQVASWIKIVD
                     FPIEEREDFYKFSNFRSCLSEVYVQLDQRNSLQDDLKEAIQSFQY"
     gene            complement(1126..1724)
                     /locus_tag="VWZ30_002185"
                     /pseudo
     CDS             complement(1126..1724)
                     /locus_tag="VWZ30_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSpn10 family transposase"
BASE COUNT          514 a          275 c          345 g          590 t
ORIGIN      
        1 ttccctaatc aatttgtata gtttttattt cactttatta taactagttt ttcgcaattt
       61 ttgagacagg aatgtgattt ttgtgataaa gttctatcat tacaagttca tttgtggtaa
      121 tatggaataa atcatttatg tatcctttca gaaatgtgct gtttctctga atataaatgt
      181 ctttttctct ttaacttaat tttacaaatt gctaaagtca tttaaggaca gctttttgaa
      241 cagttgctag aaatgtagag gtattgacca tgtatcaaaa gttacttaga aaaatagcag
      301 aagaaaaacc aagttataac caggaagaaa tccagtggct acttgatcat ttgggagatc
      361 cttctccaga aattcgtgat gaccttgttt ttacaagctt tgctagaggg attcaggaag
      421 agctatttac acaggaacaa ttttatttca ttgctgaagg gatttcatct gatggagggt
      481 tagataaaga tattgataag gtaggcttgt caacacttga acgttctttt agggcgctta
      541 tttatgtaaa tctcttgtct gctgatgcca accagcaatc gattttctat cagagattaa
      601 aatcagagat tcgcaatgtc cttttaaatc aaggcttgta ttatctttca aaagaaaagg
      661 atacaagggg tttttcaagt cagtatggtt gggttcatgc ttttgcacat ggagccgatt
      721 tactgacaga ggtggtttgt catccaaact ttcctaaaac cagagttcat gaagtatttg
      781 acatacttgg tcaattattt aaaagaatta ccattcgttt tacagatgat gaagattggc
      841 gtttagccag agtgctttat gaaccgattt tacaagggaa gttggagcaa gagcaagtag
      901 cttcttggat aaaaattgtt gattttccga tagaagaaag ggaagatttt tacaaatttt
      961 ccaatttcag atcctgtctc tcagaagtct atgtacaact cgaccagaga aatagtttac
     1021 aagatgactt gaaagaagct atccagtctt ttcaatacta gggataagct aatagctcta
     1081 tgattttcaa aaaatttcca atcaattttc ttgaaaccct ttccctgcca gaagtccgag
     1141 aatgacattg actgaatctt gttggagttt agagaggcga taagaacctc tgatagcggc
     1201 ttgaatggtt tgagcaagct tctcagagtc cttaaaatgc cctttaccca gttgttggag
     1261 ccaatcagct aattcttgaa gaggcatgtt agcaagttcg tctaaggtca tctcatctgt
     1321 tatcaaggtc atcatcgttg aactccatag gttagtagag agttcttctt tcttgaattg
     1381 ctaagccaag gtattgcact tgtagtagat attttcaatg aagtgctgtt tggttctggt
     1441 taattgctcg atgatttgga gtctggttct tgtgagatgt tggagagcca tgtatttctc
     1501 ttcttttaag aaagaaggtg aaaatcgctc aatacggaaa taatccgcaa tgtagaaagc
     1561 atcaatggta tcgtttttgt tttcttcgaa agcatcccga tatttcttga ttttattagg
     1621 ttgttcgacc ataatctcaa catgtagttc tttcaactga ctatcttcat ggaagaacat
     1681 agcagggtgg aaactgtaga gactggttgc ttccatgcca atga
//
LOCUS       DAVKDZ010000160         1670 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000160 DAVKDZ010000000
VERSION     DAVKDZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.162"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..1658
                     /locus_tag="VWZ30_002186"
     CDS             <1..1658
                     /locus_tag="VWZ30_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HEU9335824.1"
                     /translation="NDWFKDATKKNVYIVEPETSIEDFKNKKHRAYDGLNNDVHSRMI
                     LPLLNLKKAHIFLISTYNTMAYSAFEKYGKNTEEARNEFKKEIDKVAKAQQDYLDFWG
                     RLASDKVRNKLLKSQNVVPSPVWDNMNAPGYGWLDKYGYKDGSSDYAPSRELFGPTGR
                     YFPPNWNMGAMAKIWDNPYKEESVYYMVTDMISDFGISAFTHETTHINDRLAYLGGWR
                     HREGTFVEAFAQGMLQSPSVSNPNGEYGALGINMAYERNNDGNQWYNYNPNKLKNRQE
                     IDDYMKRYNEAMMLLDYVEAESVLSKNLSDNSQWFKKVDRKMREKGSYNNNLVAPNQW
                     DLVRDLNEDEKVIKLNTIKDLVDNNFATRHGVGNGVFKPEDFTPNSAYVTVNMMAGIY
                     GGNTSEGAVGALSFKHNTFRMWGYFGYEKGFVGYASNKYKDIANKENNGLLSDKLIIQ
                     KVSEGRFNTLEEWKNSWYEEIYNKATTKGFVPISVDNEQISTYARLKELFNEAVQKDL
                     NELSNQTIKNKYRHTVALKEKVFKQLLKESDGFSGDLFNTSQA"
BASE COUNT          657 a          216 c          302 g          495 t
ORIGIN      
        1 taaacgattg gtttaaagac gcaactaaga aaaatgttta tattgttgaa ccagaaactt
       61 caattgaaga tttcaagaat aaaaaacata gagcatatga tggtctaaac aatgatgttc
      121 atagcagaat gattttacct ttattaaatc ttaaaaaagc acatatattc ttaatttcaa
      181 catataatac aatggcatat agtgcttttg aaaaatatgg taaaaacaca gaagaagcta
      241 gaaatgaatt caaaaaagaa attgataaag ttgctaaagc acaacaagat tacttagatt
      301 tctggggtag actagcttcc gacaaagtaa gaaataaatt actaaaaagt caaaatgtag
      361 ttccttcacc tgtctgggat aatatgaacg cacctggtta tggttggcta gataaatatg
      421 gatataaaga tggttcttct gattatgctc cttcaagaga attatttggt cctactggaa
      481 gatatttccc accaaactgg aatatgggag caatggctaa aatctgggat aatccataca
      541 aagaagaatc tgtttactat atggttacag atatgattag tgattttggt atctctgctt
      601 tcacacacga aacaactcat attaatgata gattagctta tcttggagga tggcgacaca
      661 gagagggaac ttttgtggaa gcttttgctc aagggatgtt gcaatctcca tctgtttcta
      721 atcctaatgg tgaatatgga gcccttggta tcaacatggc ttatgaaaga aataatgatg
      781 ggaaccaatg gtataattac aatccaaata aattaaaaaa tagacaagaa attgatgatt
      841 atatgaaacg atataatgaa gcaatgatgt tactagacta tgttgaagct gaatctgttt
      901 taagtaaaaa cttatctgat aacagtcaat ggtttaagaa agttgacaga aaaatgcgtg
      961 aaaaaggaag ttataacaat aatttagttg ctccaaacca atgggattta gttcgcgacc
     1021 ttaatgagga cgagaaagta attaaattaa atactataaa agatttagta gataataact
     1081 ttgctactag acatggtgtt ggaaacggtg tgtttaaacc agaggacttc actccaaact
     1141 ctgcttatgt aacagtaaat atgatggcag gtatttacgg aggaaacaca agtgaaggtg
     1201 ctgttggtgc attgtcgttt aaacacaaca catttagaat gtggggatat tttggatatg
     1261 aaaaaggttt tgttggatat gcttcaaata aatacaaaga tattgctaat aaagaaaaca
     1321 acggcttact aagtgataag ttaatcatcc aaaaagtttc agaaggtaga ttcaacactc
     1381 ttgaagaatg gaaaaattct tggtacgaag aaatatataa taaagctaca actaaaggat
     1441 ttgttcctat atctgttgat aatgaacaaa tttcaactta tgctagatta aaagaattat
     1501 ttaatgaagc agtacaaaaa gatttaaatg aattgtctaa tcaaacaatt aaaaataaat
     1561 atagacatac tgttgcatta aaagaaaaag tctttaaaca attactaaaa gaatcagacg
     1621 gtttctctgg tgatctattt aatacatcac aagcataaga tgaataaaat
//
LOCUS       DAVKDZ010000161         1660 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000161 DAVKDZ010000000
VERSION     DAVKDZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.163"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            44..1644
                     /locus_tag="VWZ30_002187"
                     /pseudo
     CDS             44..1644
                     /locus_tag="VWZ30_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
BASE COUNT          560 a          275 c          306 g          519 t
ORIGIN      
        1 tatatcaaaa ttttagacaa tatgtttaaa aaaaggagaa actatgttta aagaatggaa
       61 agcaattttt aaaaaaccaa cctttattat tgtcataatc gggatttctc ttattccagc
      121 tctgtacaat atcatatttt tgtcatcaat gtgggaccca tatgggcaat tgtctgactt
      181 acctgtggca gttgtaaata atgataaaga ggcttcctat aatggtaata ctatggcaat
      241 aggaaaagac atggtgtcca atttaaaaga aaataaaacc ttggattttc attttgtaga
      301 tgaagaggaa ggaaagaagg gattggaaga tggcgattac tatatggtag tgactttacc
      361 aagtgattta tctgaaaaaa caactacatt atccaatatt caatcgacag cagcttatca
      421 atcattgaca agtgagcaac aaactgagat aagtgattct gtatctcaaa attttcaatc
      481 ggctcagtca attgtagctt tagtacaagg tttgcaggga agtttagaaa acttacaaaa
      541 tcaatcttct aatctttcga ctttaaaaaa tcaagctaat caagtattac ctattacttc
      601 tacttctttg ataggattgt caagtggatt aacagagata caaggagctg ttactagcaa
      661 attagttcct gccagtcagt cgattgcatc aggtgtaaac gcatatacta caggtgttga
      721 taaagtttct cagggcgcaa gtcaactaag tgaaaaaaat gccaccttga tacaaaaatc
      781 ttctagattg acagcaggag ttggttaatt acaatcagga tctgggcaat tagcagacaa
      841 atccagtcag ttactttcag gtgcttctcc attagagaat agagctaata aattggcaga
      901 tggatctggg aaactagcag aaggtggaac aaagttaact tctggattgg aagatttaca
      961 gacaggactt gcttctttag gacaaggact aggtaatgct agtgatcaac tcaaatcagt
     1021 atcaacagaa tctaaaaatg cagagatttt gtcaaatcca ctcaatcttt caaaaacaga
     1081 caatgatcaa gttcctgtaa atggaatcgc aatagctcct tatatgatat cagttgctct
     1141 ttttgttgca gcaatatcaa caaatatgat attttcgaaa ttgccttcag gacgtcatcc
     1201 agagatccgt tgggcttggt tgaaatctcg atctgaaata aatggtatta tagctgtttt
     1261 ggcaggaatt ttggtatatg gaggagttca tcttattggt ttaactgcta atcatgagat
     1321 gagaatattt attctcatca tcctaacaag tttagtattc atgtctatgg tgaccacttt
     1381 aacaacgtgg aatagccgta taggagcttt cttctcactt attttgcttt tactacagtt
     1441 agcatcaagt gcaggtactt atccacttgc tttgacaaat gatttcttta gatctattaa
     1501 tccctggtta ccaatgagct attcagtttc gggattacga caaacaatct ccatcaacaa
     1561 gtcattttcc tagctgtcat actagctcta tttactggtt taggtatact agcctatcaa
     1621 cataagaaaa tggaagaaga ttaaaaaaat cgaccgattc
//
LOCUS       DAVKDZ010000162         1623 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000162 DAVKDZ010000000
VERSION     DAVKDZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.164"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(375..1571)
                     /gene="tuf"
                     /locus_tag="VWZ30_002188"
     CDS             complement(375..1571)
                     /gene="tuf"
                     /locus_tag="VWZ30_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9335825.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
BASE COUNT          450 a          324 c          337 g          512 t
ORIGIN      
        1 gtataatgct ttttgagttt agtctagtat tatactcttc gaaaatctct tcaaaccgcg
       61 tcagcttcgc cttgtcgtat agatgttact gacttcgtca gttctatcta caacctaaaa
      121 acagtgtttt gagcagtctg cagctaactt tctataacaa aatgaaataa gaacaagaca
      181 aatcaatcaa gacaatcaaa tcgatttcta acaatgtttt agaagtagat gtgtactatt
      241 ctagtttcaa tatattatag tttgctcttt gattttcatt gagtattagt tcaatatcta
      301 aaaaaagaac cttgccaagc aagattcttt tagtgtctga cttaaataat tgttcttctg
      361 ggaactaaat cgaattaagc ttcgatttct gtaaccatac ctgaaccaac agtacgtcca
      421 ccctcacgga tagagaatgt agtaccttgt tctacggcga ttgggtgaat caactcaacg
      481 tcgattgtca cgttatcacc aggcattacc atttcagtac ctgctggaag ttcgattgaa
      541 cctgtaacgt cagtagtacg gaagtagaat tgtggacggt agttgttgaa gaatggagtg
      601 tgacgtccac cttcttcttt agtaaggatg tagacttcac ctttgaattt agtgtgtggg
      661 ttgattgaac ctggtttagc gataacttgt ccacgttcga tttcatcacg ttgaacacca
      721 cgaagaagga cacctacgtt atctccagca agaccttcgt caagttgttt acggaacatt
      781 tcaacaccag taacaactgc tttttgagtt tcttctttga taccaacgat ttcgatttcg
      841 tcgttgactt taacgatacc acggtcgata cgtcctgaag caactgtacc acgtccagtg
      901 attgagaata cgtcctcgac tggaagaagc aatggtttat cagtgtcacg ttctggttct
      961 gggatatact catcaactgt gttcatcaat tccataacga tgtcttcgta tttagagtca
     1021 ccttcaagag ctttaagtgc tgaaccttgg ataactggaa gatcgtcacc tgggaagtcg
     1081 tattctgaca ataggtcacg gatttccatt tcaaccaatt caagcaattc ttcgtcgtca
     1141 accaagtcaa ctttgttcat gaagacgata aggtgtttaa caccaacctg acgtgaaaga
     1201 aggatgtgct cacgagtttg tggcattggt ccgtcagttg aagctactac aaggatagct
     1261 ccgtccattt gagcagcacc agtgatcatg tttttaacgt agtccgcgtg tcctggagcg
     1321 tcgatgtgag cgtagtgacg tttttcagtt tcgtactcaa cgtgcgcagt gttgatagtg
     1381 ataccgcgtt cgcgttcttc tggagcagca tcgatagacg catagtcttt aggttggtta
     1441 actgatgaag gcaagcgacg tgccaaaaca gttgtgatag ctgcagttag ggtagtttta
     1501 ccgtggtcaa cgtgtccgat agtaccaatg ttaacgtgtg gtttactacg atcgtatttt
     1561 tcttttgcca tttgagtaaa agcctccaat aaaatatatt ttatagatag acagtaggca
     1621 ata
//
LOCUS       DAVKDZ010000163         1614 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000163 DAVKDZ010000000
VERSION     DAVKDZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.165"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            150..526
                     /locus_tag="VWZ30_002189"
                     /pseudo
     CDS             150..526
                     /locus_tag="VWZ30_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810398.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            691..990
                     /locus_tag="VWZ30_002190"
     CDS             691..990
                     /locus_tag="VWZ30_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEU9335826.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            1251..1591
                     /gene="tnpB"
                     /locus_tag="VWZ30_002191"
                     /pseudo
     CDS             1251..1591
                     /gene="tnpB"
                     /locus_tag="VWZ30_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          553 a          315 c          288 g          458 t
ORIGIN      
        1 aagattatac cacattgtgt actatttttg gttcatttta ctataataat aacgatacta
       61 gataatgagg aaaatgcaat ggcaaaccca actttcggag aaaaaaagcg aatacagact
      121 atattgctcg ctatggcgta tatgcagtta tccctaatcc tgaacaaaaa caaattgttc
      181 ttgttcaaca accaaatggt gcttggttcc taccatgtgg agaaattgaa gcaggtgaaa
      241 atcatcagga agccctaaag cgtgagttga ttgaagagct tggtttcaca gcagaaattg
      301 gtacctatta cggacaagtt gacgaatatt tctattctcg tcatcgtgat acctactact
      361 acaatcctgc ctacctctat gaagcaactc ctttcaaaga agtacaaaag ccactagaaa
      421 actttaatca tattgcctgg ttcccgacga ggctatccaa aaccttaaac gtggtagcca
      481 taaatgggcc attgaatctt ggaaaaaaca gcataagatt ggctaatact cttcgaaaat
      541 aaaattcaaa ccacgtcagc gtcgccttac cgtactcaag tataggctgc ggctagcttc
      601 ctagtttgct ctttgatttt cattgagtat aaatcaagcg actttattca aaaagtgcta
      661 taatagaatt agaaaatcgg aggaaatatt atgaagctta tcaatacgac aaactcacac
      721 tcgcaacttg taaaaagtca gctagaaagt accgatgcaa cccttgttga ggtttattct
      781 gcagggaata cagatgttat ttttacccaa gctccgcttc actatgaaat cctcatctca
      841 aacaaacacc gtgctattcg tgagcctgaa atcgaaacca ttcaagaatt tttcttgaaa
      901 cgtaaaattg ataaggcctc tgttgatgaa gccaatatca agacactcta ctcagagaaa
      961 ttaattggaa tttcaatccc aatcaagtaa gaaaattgag taaaaactcg atttgagaga
     1021 aagtccaata agtctttcca taataaaacg catagtagca agagtttcta cacccactat
     1081 tatgcgtttt tatcattttg aaatctactg ctaatcaatg aagaagcaaa gatcaaacta
     1141 ggcagctagt tacaagttac gtaagcgcgt cataacaagg tatctatcct aattccccaa
     1201 cttccagtat actagataaa aaataattat cagaggaaaa gtattgtact atgatgattc
     1261 tactaagtga tttaggtcaa gttcaccttg tttgtggcaa gacagatatg aggcagggaa
     1321 tagactcatt agcctatgta gttaaaaccc actttgaatt ggatcctttc tccggtcaag
     1381 tctttctctt ttgtggtgga cgtaaagacc gctttaaagc cctttactgg gatggtcaag
     1441 gattttggct actatataaa cgctttgaga acggaaaact gacttgacca agtacagaaa
     1501 aggatgtcaa agctctcaca ccagaacaag tagactggct tatgaagggc ttttctatca
     1561 ctccaaaaat aaatccatca gaaagtcgtg atttctattg aaatgaggac tttc
//
LOCUS       DAVKDZ010000164         1578 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000164 DAVKDZ010000000
VERSION     DAVKDZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.166"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            350..1477
                     /locus_tag="VWZ30_002192"
     CDS             350..1477
                     /locus_tag="VWZ30_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_135354310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9335827.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKKDSQTRVVKKGTKPQVLVQEIPIETEYLDGPTLDKGQEVGEIG
                     KITLTTIYAVDERDGTIEETTSRQITKEMVKRRIRRGTREPEKVVVPEQSSIPSYPVS
                     VTSNQGTDVAVEPAKAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGS
                     MKVNQWFQVGGKWYYVNASGELAVNTSIDGYRVNDNGEWVR"
BASE COUNT          579 a          217 c          338 g          444 t
ORIGIN      
        1 ttctatttat actgcatagt tgtataagga atgatgaaag atgagtgaaa attatcaagt
       61 tggaatgttt gtatctaaat atattagcat gtatttagat aagatgtctg caatccttta
      121 tatatgagat tgattacgat atagttaaag tgttagttca ataatcaata tatcattcta
      181 agtctatttt atatataaag tatattgttt aacgtttgat agaaaagatg ctatactaga
      241 agtaggatgt aatggcaaaa tctaatacta ataagcgcta tgcaatacgt aaactcggaa
      301 agatttttgg ttctgcggta atcgggctaa gtttattttt aggaggaatc tgaatacaag
      361 ttttgttcat gctgctgatg ggattcaata tgtcagagat gatactagag ataaagaaga
      421 gggaatagag tatgatgacg ctgacaatgg ggatattatt gtaaaagtag cgactaaacc
      481 taaggtagta accaagaaaa tttcaagtac gcgaattcgt tatgaaaaag atgaaacaaa
      541 agaccgtagt gaaaatcctg ttacaattga tggagaggat ggctatgtaa ctacgacaag
      601 gacctacgat gttaatccag agactggtca tgttaccgaa caggttactg ttgatagaaa
      661 agaagccacg gatacagtta tcaaagttcc agctaaaagc aaggttgaag aagttcttgt
      721 tccatttgct actaaatatg aagcagacaa tgacctttct gcaggacagg agcaagagat
      781 tactctagga aagaatggga aaacagttac aacgataact tatgatgtag atggaaagag
      841 tggacaagta actgagagta ctttaagtca aaaaaaagac tctcaaacaa gagttgttaa
      901 aaaaggaacc aagccccaag ttcttgtcca agaaattcca atcgaaacag aatatctcga
      961 tggcccaact cttgataaag gtcaagaagt aggagaaatt ggtaaaatta ctcttactac
     1021 aatctatgct gtagatgaac gtgatggaac aattgaagaa actacttctc gtcaaattac
     1081 taaagagatg gtaaaaagac gtataaggag agggacgaga gaacctgaaa aagttgttgt
     1141 tcctgagcaa tcatctattc cttcgtatcc tgtatctgtt acatctaacc aaggaacaga
     1201 tgtagcagta gaaccagcta aagcagttgc tccaacaaca ggctggaaac aagaaaatgg
     1261 tatgtggtat ttttataata ctgatggttc catggcaaca ggttgggtac aagttaatgg
     1321 ttcatggtac tacctcaaca gcaacggttc tatgaaagtt aatcaatggt tccaagttgg
     1381 tggtaaatgg tattatgtaa atgcatcggg tgagttagcg gtcaatacaa gtatagatgg
     1441 ctatagagtc aatgataatg gtgaatgggt gcgttaacag attcctccct gcgacaaaat
     1501 tgataatagt atgtctgaat atataattat atataaaaag ctatagataa ggtcatctta
     1561 tagcgactat aaaaaagt
//
LOCUS       DAVKDZ010000165         1573 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000165 DAVKDZ010000000
VERSION     DAVKDZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.167"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            22..939
                     /gene="adcAII"
                     /locus_tag="VWZ30_002193"
     CDS             22..939
                     /gene="adcAII"
                     /locus_tag="VWZ30_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEU9335828.1"
                     /translation="MKKQNLFLVLLSIFLVCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLDRVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQSKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            947..>1573
                     /locus_tag="VWZ30_002194"
     CDS             947..>1573
                     /locus_tag="VWZ30_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9335829.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQAKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKKEGINAEQIVIKITAQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQEH
                     SSNHGVDSNDKAVVAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGN"
BASE COUNT          533 a          268 c          350 g          422 t
ORIGIN      
        1 taaatggtta aggagttgtt catgaagaaa caaaatttat ttttagtctt gttaagtatc
       61 tttcttgtat gcttgggagc ttgtggtcaa aaagaaagtc agactgggaa agggatgaaa
      121 attgtgacca gtttttatcc tatctatgcc atggtcaagg aggtttctgg tgacttgaat
      181 gatgttcgga tgattcagtc gagtagtggg attcactcct ttgaaccttc ggcaaatgac
      241 atcgcggcca tctatgatgc agatgtcttt gtttaccatt ctcatacgct agaatcttgg
      301 gcaggaagtt tagatcccaa tctaaaaaaa tctaaagtga aggtcttaga ggcttcagag
      361 ggaatgactt tagaccgcgt cccagggcta gaagatgtag aagcagggga tggagttgat
      421 gaaaaaacgc tctatgaccc tcacacttgg ctagatcctg aaaaagctgg cgaagaagct
      481 cagattatcg ctgataaact ttcagaggtg gatagtgagc ataaagaaac ttaccagaaa
      541 aatgcgcaag cctttatcaa aaaagctcag gaattgacta agaaattcca gtctaaattt
      601 gaaaaagcga ctcagaaaac atttgtaaca caacatacag ccttttctta tttagctaag
      661 agatttggac tcaatcaact tggtattgca ggtatctctc ctgaacaaga accaagtcca
      721 cgacaactaa cagaaattca ggaatttgtt aagacctata aggttaaaac gatttttaca
      781 gaaagtaacg cttcttcaaa agtagctgaa actcttgtca aatcaacagg tgtgggtctt
      841 aaaactctga atcctttaga gtcagaccca caaaatgaca agacctattt agaaaatctt
      901 gaagaaaata tgagtattct agcagaagaa ttaaagtgag gaaagaatga aaatcaataa
      961 aaaatatcta gcaggttcag tggcagtcct tgccctaagt gtttgttcct atgaacttgg
     1021 tcgtcaccaa gctggtcagg ctaagaaaga gtctaatcga gttgcttata tagatggtga
     1081 tcaggctggt caaaaggcag aaaatttgac accagatgaa gtcagtaaga aagaggggat
     1141 caacgcagaa caaattgtta tcaagattac ggctcaaggt tatgtgacct cacatggaga
     1201 ccattatcat tactataatg gcaaggttcc ttatgatgcc atcatcagtg aagaacttct
     1261 catgaaagat ccgaattatc agttgaagga ttcagacatt gtcaatgaaa tcaagggtgg
     1321 ctatgtgatt aaggtagacg gaaaatacta tgtttacctt aaagatgcgg cccatgcgga
     1381 taatattcgg acaaaagaag agattaaacg tcagaaacag gaacatagta gtaatcacgg
     1441 tgtcgattct aacgataagg cagtagttgc tgccagagct caaggacgtt atacaacgga
     1501 tgatgggtat atcttcaatg catctgatat cattgaggac acgggtgatg cttatattgt
     1561 ccctcacggc aat
//
LOCUS       DAVKDZ010000166         1542 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000166 DAVKDZ010000000
VERSION     DAVKDZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.168"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            1..378
                     /locus_tag="VWZ30_002195"
                     /pseudo
     CDS             1..378
                     /locus_tag="VWZ30_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263388.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
     gene            complement(446..526)
                     /locus_tag="VWZ30_002196"
                     /pseudo
     CDS             complement(446..526)
                     /locus_tag="VWZ30_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            710..>1542
                     /locus_tag="VWZ30_002197"
     CDS             710..>1542
                     /locus_tag="VWZ30_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEU9335830.1"
                     /translation="MYITAKEAAKKWGISDRRVRILCSEGKIHGAYQEGRNWKIPCDA
                     VKPTDGRYKTKESLILVLGDKLETLKKRRPLTEGEVERLNEEFLVEYTYNSNAIEGNT
                     LTLRETDMVLRGLTIDRKSLKEHLEVIGHKDAFDYVRQLVRENAPISEKIIKDIHYLV
                     LADKKEDRGVYRRVPVRIMGAAHEPVQPYLIVPKMEELLERYENSKEDIVTRLARFHI
                     EFESIHPFIDGNGRTGRLLVNLELMKAGYPPIDIKFTDRLKYYEAFDHYHLKDDLSAM
                     A"
BASE COUNT          474 a          271 c          338 g          459 t
ORIGIN      
        1 gacttccttc ctcgtgtaca tggttcaggt ctctttactc gtgggcaaac tcaagctctt
       61 tcagtcttga ccttggctcc gatgggagaa actcaaatca ttgatggttt ggatccagag
      121 tacaagaaac gctttatgca ccactataac ttccctcaat attctgtagg ggaaacaggc
      181 cgttacggtg cgccaggtcg tcgtgaaatc ggtcacggtg ctcttggtga gcgtgctctt
      241 gctcaagtct tgccaagctt ggaagaattc ccatacgcta tccgtctagt agcagaagtt
      301 ttggaatcaa acggttcttc atctcaagct tctatctgtg cgggaactct tgcccttatg
      361 gctggtggtg tgccaattac tcttagaaaa tctcttcaaa ccacgtcaac gtcgccttgc
      421 cgtgcgtatg gttactgact tcgtcagttc tattcacaac ctcaaaacag tgttttgagc
      481 aacctgcggc tagtttccta gtttgctctt tggttttcat tgagtataac acattgttag
      541 aagttggttt aaattcccta atcaatttgt tcacatttac cttcgatata ttatatccca
      601 tagttatagt ggaaaacact attgttcgga taaatggtag atgttattcc gactcccttg
      661 ctattgttcc gacaaggtga tataatgtga cagaacggag ggataaaaaa tgtatattac
      721 agcgaaagag gccgctaaaa agtgggggat atctgacaga agggtaagaa ttttgtgctc
      781 agaagggaag attcacggtg catatcaaga gggaaggaat tggaaaatac catgtgacgc
      841 agtaaagccg acagatggga gatataagac taaagaatca ctcattctag tcttgggaga
      901 taagttagaa actctaaaaa aacgacgtcc tttaactgaa ggagaagtgg aaagattaaa
      961 tgaagaattt ttagtagaat atacatacaa ttctaatgct attgaaggaa atacactgac
     1021 tttacgtgaa acagacatgg ttcttagagg acttacaatt gaccgaaaat ccttaaaaga
     1081 gcatttggag gtcattggac ataaagatgc ttttgattat gtaaggcaac ttgttcgtga
     1141 aaatgctcca atatctgaaa aaattataaa agatattcac tatttggttt tggcagacaa
     1201 gaaagaagat agaggtgtgt atcgaagagt tccagttcga attatgggag cggctcatga
     1261 acctgtgcaa ccctatttaa ttgttcctaa gatggaagag ttactggaac gatatgaaaa
     1321 tagcaaagaa gatattgtta ctagacttgc ccgttttcat attgagttcg aatctattca
     1381 tcccttcatt gatggaaatg gaagaacagg gaggttgttg gttaatttgg aactgatgaa
     1441 agcaggttat ccacctattg atataaaatt tactgataga ttaaaatatt atgaagcttt
     1501 tgaccactat catttaaagg atgatttatc agccatggca ga
//
LOCUS       DAVKDZ010000167         1476 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000167 DAVKDZ010000000
VERSION     DAVKDZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.169"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(61..789)
                     /locus_tag="VWZ30_002198"
     CDS             complement(61..789)
                     /locus_tag="VWZ30_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9335831.1"
                     /translation="MAIVEIINLTKSFKDIEVIHNTSFYLNKGKVYGFVGPNGAGKTT
                     IIKMILGILKPDSGKITIFNQTVEQNSENILSRIGLVLGPSFYGHLDAYKNLKLIANM
                     KGLSLDTERLNEYLSMVGLKDVKKKKVKNFSTGMKQRLSIAASLLGSPEILIWDEPIN
                     GLDPQGVIEIRSLIRFLQEKKGITFLISSHILSELDKVISDIIIINYGKVEFFGSCHY
                     LLQKYNCRNLEEAYLACLAGGEYD"
     gene            complement(823..993)
                     /locus_tag="VWZ30_002199"
     CDS             complement(823..993)
                     /locus_tag="VWZ30_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335832.1"
                     /translation="MKKVNEYVISTAASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIFYKNNKN"
     gene            complement(1006..1452)
                     /locus_tag="VWZ30_002200"
     CDS             complement(1006..1452)
                     /locus_tag="VWZ30_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9335833.1"
                     /translation="MNRSILDNITLKHEVTSQKIEEVCKAVQIYDEIMAMPMKFNTII
                     SEMGSNISGGQRQRIALARALINNPSIVILDEATSALDTINEERITKYIKSQGCTKII
                     VSHRLSTIKDADIIFVMKGGKIVESGNHKYLMALGGEYYSLYTKRK"
BASE COUNT          481 a          283 c          165 g          547 t
ORIGIN      
        1 attattggaa aaataagaac aaaccataag atgctttttt tctgctttag aaactctaat
       61 ttaatcatat tcacctccag ctaaacaagc taagtaggct tcttccagat ttcgacaatt
      121 atacttttgt aaaaggtagt ggcaagaacc aaaaaattca actttcccat aatttataat
      181 tataatatca gatatcactt tatccagttc actaagaata tgacttgata ttaaaaatgt
      241 aatacctttt ttctcctgta gaaaacggat taaacttcta atttcaatta caccctgtgg
      301 atcaagtcca ttgattggtt catcccaaat taatatttct ggactaccta acaatgatgc
      361 agctatactt agacgttgtt tcatgcccgt tgaaaaattt ttcacttttt tctttttaac
      421 atcttttaat cctaccattg atagatattc attaagtctt tcagtatcta aactaagccc
      481 tttcatattt gctattaact tcaaattttt ataagcatca agatgaccat aaaatgaagg
      541 tcccaaaact aatcctatcc tagataatat attttcggaa ttctgttcta cggtttgatt
      601 aaaaatagtt attttcccag aatctggttt taaaatccct agaatcattt tgataattgt
      661 agtttttcca gccccatttg gtccaacaaa accatatact tttcccttat ttaaataaaa
      721 tgaagtgtta tgaataactt caatatcttt aaagcttttt gttagattta taatttcaac
      781 tatagccatc tctactcctt agtttacagc aaaaaattgt gcttaattct tattgttttt
      841 gtaaaaaatc agcgaaccca atactattcc attcaacaat cctaaagaaa taccatattc
      901 aactggaaaa tctaaaaaaa ttgcaaatac tattccaatc atcaccccta gtgaggctgc
      961 tgttgaaatt acatattcat ttactttctt cattttcttt acacctcatt tcctttttgt
     1021 atataagctg tagtactctc caccaagagc cattaagtac ttatgatttc ctgactcaac
     1081 aatcttacca cctttcatta caaaaataat atccgcatcc ttaatcgttg acaatctatg
     1141 agatacaatt attttagtac agccctgact ttttatatac tttgttattc tttcctcatt
     1201 aatagtgtct aatgcactag ttgcttcatc taaaattaca atactaggat tatttattaa
     1261 tgcacgtgcc agtgctatcc gttgtctttg accacctgaa atatttgaac ccatctctga
     1321 gatgatagta ttaaatttca tcggcatagc catgatttca tcatagattt gaactgcttt
     1381 acaaacttcc tctatctttt gtgaagtaac ttcgtgcttt aaagttatgt tatcaagaat
     1441 acttctgttc aataaaaacg aatcttgtgg aactac
//
LOCUS       DAVKDZ010000168         1408 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000168 DAVKDZ010000000
VERSION     DAVKDZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.170"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            109..>1408
                     /locus_tag="VWZ30_002201"
     CDS             109..>1408
                     /locus_tag="VWZ30_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate hydrolase"
                     /protein_id="HEU9335834.1"
                     /translation="MNKKKMILTSLASVAVLGAGFVASQPASVRAEGAQPAAEKAPTY
                     DDLLKLLKEFENDAIAAIGKRTDLTDEQKGAKRKEIKEFINKDEILKLVKDEVLTVDD
                     VLTELNAAADGASQIGRSVDKPYVGEYKNNVWAKDNANSGSEAQDLTDDQKQAINEEL
                     DKFAKRDDVQKAREALADLDEKAEKVSKKIELAKKSLETKNESVLKLKALYDEAVKSL
                     GESNELTRRLATEYSNNHDEFQALYESTQEQIEELKDYNEQISEGEETLILAIQNKIS
                     DLDDKIAEAEKKLADSQNGEGVEDYWTSGDEDKLEKLQAEQDELQAELDQLLDEVDGQ
                     EPAPEAPAEQPKPEKSAEQQAEEDYARRSEEEYNRLTQQQPPKAEKPAEEPTQPAPAP
                     EQPTEPTQPEKPVAPKTGWKQENGMWYFYNTDGSMATGWLQ"
BASE COUNT          544 a          244 c          315 g          305 t
ORIGIN      
        1 atagaaattt gtaacaaaaa tgtaatataa aacacttgac aaatatttac ggggggatat
       61 aattaatata tagtctgaaa aggattatca gaaaagaggt aaatttagat gaataagaaa
      121 aaaatgattt taacaagcct agccagcgtt gctgttttag gggctggttt tgttgcgtct
      181 cagcctgctt cggtgagagc agaaggtgct caacctgcgg cagaaaaagc tccaacttac
      241 gatgatttac tgaagctttt gaaggagttt gaaaatgatg caattgcagc aattggcaaa
      301 cgcacagact taacagatga acaaaaagga gcaaaacgta aggaaatcaa agaattcatc
      361 aataaagatg aaattttgaa attggtcaaa gacgaggttc ttacagtgga cgatgtcttg
      421 acagaactaa acgcagcagc tgatggagct agtcaaattg gacgatcagt tgataaacct
      481 tatgttgggg aatataaaaa caatgtttgg gctaaagata atgctaattc aggttccgaa
      541 gctcaagacc taacagatga ccaaaaacaa gccattaatg aagagttaga taaatttgcc
      601 aagcgtgatg atgtccaaaa agcaagggaa gctcttgcag acttggatga gaaagcggaa
      661 aaggttagca aaaagattga gctagcaaaa aaatctcttg agactaagaa tgagtcagtt
      721 ttgaagttga aagctcttta tgatgaagca gttaaatcac ttggtgagtc aaatgagctt
      781 actcgacgcc tggcaacaga gtattctaat aatcatgatg agttccaggc gctctatgag
      841 tcaactcaag aacaaatcga agagttgaaa gactacaacg aacaaatctc tgaaggagaa
      901 gaaactctta tccttgcgat tcaaaataaa atatcagatc tggatgataa aatcgcagaa
      961 gcggagaaaa agcttgctga ctcacaaaat ggagagggtg tagaagacta ttggacttca
     1021 ggtgatgagg acaaattaga aaagcttcaa gcagagcaag atgaacttca agctgaattg
     1081 gatcaattgt tggatgaagt tgatggacaa gaaccagccc cagaagcacc agctgaacaa
     1141 ccaaaaccag agaagtcagc agaacaacaa gctgaagaag actatgctcg tagatcagaa
     1201 gaagaatata accgcttgac ccaacagcaa ccgccaaaag ctgaaaaacc agctgaagag
     1261 ccaactcaac cagcaccagc cccagagcaa ccaactgagc caactcaacc agaaaaacca
     1321 gtcgctccaa aaacaggttg gaaacaagaa aatggtatgt ggtacttcta caatactgat
     1381 ggttcaatgg cgacaggatg gctacaaa
//
LOCUS       DAVKDZ010000169         1350 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000169 DAVKDZ010000000
VERSION     DAVKDZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.171"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            605..826
                     /locus_tag="VWZ30_002202"
     CDS             605..826
                     /locus_tag="VWZ30_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9335835.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLGLTDFPDKIRFRK"
     gene            1132..>1350
                     /locus_tag="VWZ30_002203"
     CDS             1132..>1350
                     /locus_tag="VWZ30_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEU9335836.1"
                     /translation="METVNYKDLVAIGFPEHTSRNIIRQAKKIAVKKFEEARKNDKNE
                     VQLGCSPFDNKRLGIAPKNIVENLISISF"
BASE COUNT          484 a          194 c          227 g          445 t
ORIGIN      
        1 tgggatttga attttccctt cttccaaaac taataaaatt gaaagaacaa aatctagttt
       61 aaatgttgct cgaaaattaa ataattgaat acgattaaca atgaaacaat acaacgtgag
      121 tcatgacttc aaagtaattg attttatgat tctggtttct tacgcttaat taagcaagtt
      181 gtattcactt tatcaaattg ttattactct aaaaagcgtc taacagctat agagtgtctg
      241 tttgcttctc tttactttta ttttaaataa ttcattaacc ttccgtaagt gcccaataat
      301 aaggtgtgtt gaagaagtct gagataaaat agttctcagt cacaaaaaaa actatagatt
      361 cccattggtg aacagctccc tgtcaagtag acagtaaaat aatataaaat cttaagcgac
      421 ctgattcctg aattccagtg gagtcaggtt gttttttaac cgtctttctt aatattgtct
      481 tacctataat agatatgctt atgaaaagtg tttaataaat taattttagt tcaaaatgtc
      541 caatttggac actttgaatg atttcttagt tttatatatt atacttttct caggagaaag
      601 ctagatgtac agacgtttga gagatttgag ggaagatcac gatttgactc aaaaacaaat
      661 agctaaaata ctttcgttta caaactcagc ttatgctaaa attgaacggg gtgagcatac
      721 gttaacagca gatgtattgg taactctctc aaacttttac gatgtcagta cagactacct
      781 attgggattg acagattttc ctgataaaat tcgctttaga aaataatctc ctcaatttca
      841 tagagtgtga aaatgagggg attttttatt tgttctttga aaattaaata gttcaaaatg
      901 gtactttcct catttgtgga gcaaatttga atggctcgcc atgataagag cgattttaaa
      961 accatcaata aaatagagcg atactttata tgccatgata caaatgatat acaatgatac
     1021 ttctgaccgt tcaggctgcc aacgtaaaag agcagcaagt gaaattctta tgatgacttc
     1081 atcagtcatg ccatggaggt gaaaaaatct aagaaaggaa gtagtaaaat catggaaaca
     1141 gtaaactata aagatttagt tgcgattggt tttccagagc acacatctag aaatattatt
     1201 agacaagcaa aaaagattgc tgtaaaaaag tttgaagaag ctagaaaaaa tgataagaat
     1261 gaggtacaat taggatgttc acctttcgat aataaaaggt taggcattgc tcctaaaaat
     1321 attgtagaaa atttaattag tatttctttt
//
LOCUS       DAVKDZ010000170         1331 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000170 DAVKDZ010000000
VERSION     DAVKDZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.172"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            34..576
                     /locus_tag="VWZ30_002204"
     CDS             34..576
                     /locus_tag="VWZ30_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU9335837.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKARISRS
                     SFYTHYKDKYDMIEYYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSIDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(617..690)
                     /locus_tag="VWZ30_002205"
     tRNA            complement(617..690)
                     /locus_tag="VWZ30_002205"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(654..656),aa:Asn,seq:gtt)
     gene            complement(696..767)
                     /locus_tag="VWZ30_002206"
     tRNA            complement(696..767)
                     /locus_tag="VWZ30_002206"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(732..734),aa:Glu,seq:ttc)
     gene            complement(819..>1331)
                     /locus_tag="VWZ30_002207"
     CDS             complement(819..>1331)
                     /locus_tag="VWZ30_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9335838.1"
                     /translation="YAIIVFITSRSEFATLTYKYQVSALDFVDKDINDEMFKKRIEQN
                     IFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILYIETTGVSHKLRIIGKNFAKEFY
                     GTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRKNLEIVFYEDHRCPISRLKIRKL
                     KDILEKKSQK"
BASE COUNT          456 a          244 c          204 g          427 t
ORIGIN      
        1 acatatagtc taatttgttt acaaggagga aatatgcaag aaagtaacaa acgcttaaaa
       61 acaaagcgaa ctattgaaaa tgctatggta caattactga tggaacagcc atttgataaa
      121 atttctactg tcaaattagt agaaaaagcc agaattagtc gttccagctt ctatactcac
      181 tataaggata agtatgatat gattgagtac tatcaaagca agctattcca tacatttgaa
      241 tatatttttc aaaaacatgc tcatcacaaa agagacgcta ttttagaagt ctttgaatat
      301 ctagagtcag aaccacttct ggctgccctt ctttctgaaa atgggactaa agaaatccaa
      361 aatttcttac gaaataaact tcatatcatg ctcagtatag atttacaaaa gcgatttatg
      421 caactgaatc taaataccac tgaattagaa tatagtagca tctatctaac tcatgcactt
      481 tttggtgtct gccaaacttg gattgcacat ggaaaaaaag aaagttctca agaaataaca
      541 gacttcctta tgaaaatgct tggtgataca aattgataca aaaagaggaa cacagctgtg
      601 ttccctttta tctatactcc gccagtagga ctcgaaccta cgacatcatg attaacagtc
      661 atgcgctact accaactgag ctatggcgga taaaatagtc cgtacgggat tcgaacccgt
      721 gttaccgccg tgaaaaggcg gtgtcttaac cccttgacca acggaccttc tatctgtagc
      781 agatataacc attatatcaa tttcttgcta attgtcaatc acttttgaga ttttttctct
      841 aaaatatctt ttaattttct aatttttaat cttgaaatag gacaacgatg gtcttcatag
      901 aaaacaattt ctaagttttt tcgatcaatt tctctgatat tgcctatatt taccaaaaat
      961 gacttgtgag gagaataaaa tcgctgagta tgtttgtcct tttcctgaat atctgtcatg
     1021 gtaccataaa actcttttgc aaaattctta ccaataatgc gcaatttatg agatacccct
     1081 gttgtttcaa tatacaaaat atcatggtaa ggaattttta aatcatttcc cttgtaattg
     1141 tagtcgaaat aatctacaac atcttcattt tcaagtaaca tactcttcgt gtagaagata
     1201 ttttgctcaa ttctcttctt aaacatctca tcattgatat ccttatcaac aaaatctagg
     1261 gctgatacct ggtatttata ggttagagtc gcaaactctg atcgactagt gataaagacg
     1321 ataatagcgt a
//
LOCUS       DAVKDZ010000171         1314 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000171 DAVKDZ010000000
VERSION     DAVKDZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.173"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            15..824
                     /locus_tag="VWZ30_002208"
     CDS             15..824
                     /locus_tag="VWZ30_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1837 domain-containing protein"
                     /protein_id="HEU9335839.1"
                     /translation="MEKMHSYIKYLIHEDPIITDEGKQVEILHLDIQDDPEIFEDWAN
                     QFRRNYCSDDELAEMIGFMNISPKEYLKNFKLPSDSGIGLSTMSGDFGEILVSDYLQN
                     IEEYVVPRTRYDSRVNKDTSTQGSDVLGYKQDSSNVSNDEVVVIEVKSSASDSSSSQA
                     KNKLQEAINHSDKDFERFSTSIVASYLRLKKSNSDQENVVERFLNITDKPFNVIYGAV
                     AVYSNQSYDIDVIKTVVSKKHRDFQKLRLLVIHSDELMKFIRELYSKASEV"
     gene            821..>1314
                     /locus_tag="VWZ30_002209"
     CDS             821..>1314
                     /locus_tag="VWZ30_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence associated protein"
                     /protein_id="HEU9335840.1"
                     /translation="MIEFGKKSLYFSKLVRSKAKMIEFEIPLESHIPISEDAQKSFLG
                     ALAIAADTARKYFEDYINHKSFDSQLKNQLHNVAEYFDALLVSGLGNSAEYQDYIAIL
                     GTTAYYLGDYNGSSRVMVNYISDDMQLLEESITLVKVFINVITDKLFLNHTPIEGKYS
                     SELNT"
BASE COUNT          471 a          164 c          244 g          435 t
ORIGIN      
        1 tatgagtaaa agtaatggaa aagatgcata gttatataaa gtatttaatt catgaagatc
       61 ctattattac tgatgaaggt aagcaggttg agattcttca cttagacatt caagatgacc
      121 cagaaatttt tgaagattgg gcaaaccaat tcagaagaaa ttattgttct gatgatgaac
      181 ttgctgaaat gataggtttt atgaatatct cccctaaaga gtatcttaaa aattttaaat
      241 taccctcgga tagtggtatt ggcctttcaa cgatgtcagg tgattttgga gaaatattag
      301 tatctgatta tcttcaaaat attgaagaat atgtggttcc taggactcgt tatgatagta
      361 gagtcaataa agatacttca actcaaggaa gtgatgttct aggatataag caagattctt
      421 cgaatgtatc gaatgatgag gttgtagtta ttgaagtaaa atcatcggca tctgattcta
      481 gtagctctca agcgaaaaat aagcttcaag aagctattaa tcattctgat aaagattttg
      541 aacgattttc aacctcaatt gtagctagct atttaaggtt aaagaaatct aatagtgatc
      601 aagaaaatgt agtagaaaga tttttaaaca taacggataa accatttaat gtaatctatg
      661 gagctgttgc agtctactcc aatcaatcat atgatattga tgttatcaag acagtagttt
      721 caaaaaaaca cagagatttt cagaaattac gactattggt cattcattct gatgaattaa
      781 tgaaatttat tagagaactt tattcgaaag cgagtgaggt atgatagaat ttgggaaaaa
      841 gtcattatat ttttcaaaat tagtgcgttc aaaggcaaag atgattgaat ttgaaattcc
      901 tttagaaagt catattccaa tttctgagga tgctcaaaaa tcgtttcttg gggcattagc
      961 aattgctgca gatactgcta ggaagtattt tgaagattat atcaatcata agagttttga
     1021 ttcacaatta aaaaatcaat tacacaatgt tgctgaatat tttgatgcat tgctggtgtc
     1081 aggattagga aattcagctg aatatcaaga ctatattgca atactaggta ccacagctta
     1141 ttatctaggc gattataatg gtagttcgag agttatggtt aactacatat cggatgatat
     1201 gcagttatta gaagagagta taactcttgt aaaagtattt attaatgtta ttacagataa
     1261 actctttttg aatcatacgc caatagaagg taaatactct agtgagttaa atac
//
LOCUS       DAVKDZ010000172         1231 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000172 DAVKDZ010000000
VERSION     DAVKDZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.174"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(208..291)
                     /locus_tag="VWZ30_002210"
     tRNA            complement(208..291)
                     /locus_tag="VWZ30_002210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(255..257),aa:Leu,seq:caa)
     gene            complement(301..372)
                     /locus_tag="VWZ30_002211"
     tRNA            complement(301..372)
                     /locus_tag="VWZ30_002211"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Gln,seq:ttg)
     gene            complement(386..458)
                     /locus_tag="VWZ30_002212"
     tRNA            complement(386..458)
                     /locus_tag="VWZ30_002212"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(423..425),aa:His,seq:gtg)
     gene            complement(473..543)
                     /locus_tag="VWZ30_002213"
     tRNA            complement(473..543)
                     /locus_tag="VWZ30_002213"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(509..511),aa:Trp,seq:cca)
     gene            complement(548..628)
                     /locus_tag="VWZ30_002214"
     tRNA            complement(548..628)
                     /locus_tag="VWZ30_002214"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(592..594),aa:Tyr,seq:gta)
     gene            complement(641..713)
                     /locus_tag="VWZ30_002215"
     tRNA            complement(641..713)
                     /locus_tag="VWZ30_002215"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(678..680),aa:Phe,seq:gaa)
     gene            complement(717..790)
                     /locus_tag="VWZ30_002216"
     tRNA            complement(717..790)
                     /locus_tag="VWZ30_002216"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(754..756),aa:Met,seq:cat)
     gene            complement(801..890)
                     /locus_tag="VWZ30_002217"
     tRNA            complement(801..890)
                     /locus_tag="VWZ30_002217"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(852..854),aa:Ser,seq:tga)
     gene            complement(973..1046)
                     /locus_tag="VWZ30_002218"
     tRNA            complement(973..1046)
                     /locus_tag="VWZ30_002218"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1010..1012),aa:Ile,seq:gat)
     gene            complement(1081..1151)
                     /locus_tag="VWZ30_002219"
     tRNA            complement(1081..1151)
                     /locus_tag="VWZ30_002219"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1117..1119),aa:Gly,seq:tcc)
     gene            complement(1157..1229)
                     /locus_tag="VWZ30_002220"
     tRNA            complement(1157..1229)
                     /locus_tag="VWZ30_002220"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1194..1196),aa:Val,seq:tac)
BASE COUNT          355 a          317 c          270 g          289 t
ORIGIN      
        1 aaacgaatta aggtaaaact aaaaggactt agccctgtac aatacagaac taaatccttt
       61 gaataaatta attgtctaac ttttgggggt cagtacactt ttaatcatgt gcttttatat
      121 gaaattagta attattagta acaattccaa gaaatttctt atggcataac aaaaagacaa
      181 ggtttctaac ccttgtcttt tctactatac cggcggccgg ggtcgaaccg gcacgtcctt
      241 gcggacactg gattttgagt ccagcgcgtc tgccaattcc gccacgccgg caaatagtaa
      301 ctggggtagc tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt
      361 ggctataccc caataatata aaataggcga gtgatgggga tcgaacccac gcatgccaga
      421 gccacaatct ggtgtgttaa ccacttcacc acacccgcca taattctatt aacacgggca
      481 gtaggaattg aacccacact gaaggttttg gagaccttag ttctaccttt aaactatgcc
      541 cgtaaaatgg aaggggaggg attcgaaccc ccgaacccga aggagcggat ttacagtccg
      601 ccgcgtttag cctcttcgct acccttccaa aatatataaa tggcgcgaga cggaatcgaa
      661 ccgccgacac atggagcttc aatccattgc tctaccaact gagctaccga gccttattgc
      721 gggagcagga tttgaaccta cgaccttcgg gttatgagcc cgacgagcta ccgagctgct
      781 ccatcccgcg ttaatataaa aggaggatgt gggattcgaa cccacgcacg cttttacacg
      841 cctgacggtt ttcaagaccg ttcccttcag ccggacttgg gtaatcctcc aaaatagttt
      901 atacgggaac aagcccgtgt cctcttagtg aatagataat attccaatcc cctagtaatg
      961 gactaaacac tatgggcacg agtggactcg aaccaccgac ctcacgctta tcaggcgtgc
     1021 gctctaacca cctgagctac gcgcccaagt caaaaacttg gtacaaagaa caaagttcaa
     1081 agcgggtgac gagaatcgaa ctcgcgacaa cagcttggaa ggctgtagtt ttaccactaa
     1141 actacacccg ctaaaatggg agttaacggg atcgaaccgc tgaccctctg cttgtaaggc
     1201 agatgctctc ccagctgagc taaactccct a
//
LOCUS       DAVKDZ010000173         1089 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000173 DAVKDZ010000000
VERSION     DAVKDZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.175"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            1..1089
                     /locus_tag="VWZ30_002221"
                     /pseudo
     CDS             1..1089
                     /locus_tag="VWZ30_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          414 a          187 c          210 g          278 t
ORIGIN      
        1 gatatagctt agagaagctt tcaaataaat ttgggataaa caattctaat cttaggtaca
       61 tgattaaatt gattgatcgt tacggaatag agttcggcaa aaaaggaaaa aatcgttact
      121 attctcctga tttaaaacaa gaaatgattc ataaagtctg acatgaaggc tggactaaag
      181 atagagtttc tcttgaatac ggtctcccaa gtcgtacgat acttcttaac tggctagcac
      241 aatacaggaa aaacgggtat actattgttg agaaaccaag agggagagta cctaaaatgg
      301 gacgtaatcc aaaaacgaga cctgaagaga ggacagaatt agaacgtctt caatcagaaa
      361 atgagtacct gagagcggag aatgccatcc taaaaaagtt aagagaactc cgattgaagg
      421 aggaaaaaga gaaagaagaa agacagaaat tgttcaagaa ttaatgactg agttttcgtt
      481 agctcttctt ctaaaagcca ttaaactagc tcgttcgacc tactactatc acttgaaaca
      541 gctagataaa ccagataagg accaagagct taaagctgaa attcaatcca tctttatcga
      601 acacaaggga aattatggtt atcgtcgaat ttatttagaa ctaagaaatc gtggttatct
      661 ggtaaatcat aaaagagttc aaggcttgat gaaagtactc aatttacaag ctaaaatgcg
      721 acagaaacga aaatattctt ctcataaagg agatgttggc aaaaaggcag agaatctcat
      781 tcaacgtcaa tttgaaggct ctaaaacaat ggaaaagtgc tacacagatg tgacagaatt
      841 tgccattcca gcaagtactc aaaagcttta cttatcacca gttttagatg gctttaatat
      901 cgaaattatc gcctataatc tttcaacttc atccaactta gaataagtac aaacaatgtt
      961 ggaacaggca ttcacagaga agcactacga gaatacgatt ctccatagtg accaaggctg
     1021 gcaataccaa cacgattctt atcatcggtt cttagagggt aagggaattc aagcatccat
     1081 gtcacgtaa
//
LOCUS       DAVKDZ010000174         1028 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000174 DAVKDZ010000000
VERSION     DAVKDZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.176"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            2..736
                     /locus_tag="VWZ30_002222"
     CDS             2..736
                     /locus_tag="VWZ30_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9335841.1"
                     /translation="MVAFYNSKTFLSRILEILGEETEINGVVKHDLNGEIALKNVSFS
                     YTRDSKKVLSNVSIEIKKGEKVAIVGSSGSGKSTLSKLLIGLFPASSGSILFDDLDYN
                     LLDKQYLRQQIGIVPQDMTLFNKTIHENIAGDISVSEEEMTKICKLVNIHDEIMEMPM
                     GYNTLVSEMGMNLSGGQRQRIILARALVKKPKIILLDEATSYLDNVNENEIMQKFKEQ
                     NITIIVIAHRLSTIIDSNQIFVMDKV"
     gene            complement(872..>1028)
                     /locus_tag="VWZ30_002223"
     CDS             complement(872..>1028)
                     /locus_tag="VWZ30_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU9335842.1"
                     /translation="KDFYKKKYHEVPKHQHKRAIVLTARKFVRLVVVLLRNHQLYTPP
                     RRLMEDK"
BASE COUNT          352 a          154 c          199 g          323 t
ORIGIN      
        1 gattgtagca ttctacaata gcaaaacatt tttatctcgg attttagaga ttttaggtga
       61 agaaactgaa ataaatggtg tagtgaaaca cgacttaaat ggggaaatcg cacttaaaaa
      121 tgtttcattt tcatatacta gagatagtaa aaaagtactc tctaatgtgt ctattgaaat
      181 aaagaagggg gaaaaggtag caattgttgg ctcttctgga tcaggtaaaa gtaccctttc
      241 caaattattg ataggtttat ttccagcatc ctcaggtagt attttatttg atgacttaga
      301 ttataatttg ttggataaac agtatcttcg tcaacaaatt ggcattgtcc cacaagacat
      361 gactttattt aataaaacca tacatgagaa tattgctgga gacatctcag tctcagagga
      421 agaaatgaca aaaatttgta agttagttaa tatccatgat gaaattatgg aaatgcccat
      481 ggggtataat actttagttt cagaaatggg aatgaatctg tcaggagggc aacgtcagag
      541 aattatttta gcaagagcgc tagttaagaa acctaaaata atacttttag atgaagccac
      601 aagttatcta gataatgtta atgaaaacga gattatgcag aaatttaaag aacaaaatat
      661 cacaattatt gttattgcac atcgactttc taccattatc gattctaacc agatttttgt
      721 aatggataaa gtctagtggt aaaatgtcaa gcgacccccc gctcaaagtt tgaaaactat
      781 ttcaaaaaaa gggtgcacta aagaagccct actgaaaacg atgttttcaa actgttttca
      841 gtagattttc aaggagaggt gcagatataa cttacttatc ttccataagc ctccttggtg
      901 gcgtatagag ttgatggttg cgtagtagca caaccaccag acgcacaaat tttctagcgg
      961 ttagtacgat ggctcgtttg tgttggtgtt taggtacttc atggtatttc ttcttataga
     1021 aatccttg
//
LOCUS       DAVKDZ010000175         1024 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000175 DAVKDZ010000000
VERSION     DAVKDZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.177"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            83..>1024
                     /locus_tag="VWZ30_002224"
     CDS             83..>1024
                     /locus_tag="VWZ30_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9335843.1"
                     /translation="MKIKKILIKNFKNIKETRIIDFQENVTLFVGPNGFGKTTIFDAI
                     ELSLTGKIRRIEESDYSDGRSSFSAPYFQNNPNQNTFIELMLTNDEGNSLIVSSLYKS
                     SMNTEGSNVPKFSFSKFQRRVRVGPGISFQSDEDFEGSSERDIQKDISQFLGYESEDY
                     SLGDTFDLFHYIQQDETAYYLKQKEKDRKEQLNFLLNIGNYVNRKNRLERLRSTLSSS
                     EKELRAKKGKLKNSPIKNNILYSKLSLRADTDFAFNNKSIIFDEELSNESLNIYLSEI
                     TKLQEFRNSFSPKDYFLRKQSEQFEREVLDDKDFTIYL"
BASE COUNT          402 a          117 c          173 g          332 t
ORIGIN      
        1 agaaattatc aaaatcacta aaagatattt ctaccgatga tgataatatt tctgatttac
       61 tacaaatagg agaattatta caatgaaaat caaaaaaatt ttaataaaaa actttaaaaa
      121 tataaaggaa actagaatta ttgattttca agagaatgta actttgtttg ttggaccaaa
      181 tggatttgga aaaactacaa tttttgacgc aatagagtta tcattaactg ggaaaattcg
      241 tcgaattgaa gagtccgatt atagtgacgg tagaagtagt tttagtgctc catattttca
      301 aaataatcct aatcaaaata cattcattga gcttatgttg acaaatgatg agggtaattc
      361 tttgatagta tctagtcttt ataagagtag tatgaatact gagggcagca atgtaccaaa
      421 gttttcattt tctaaatttc aaagaagagt tcgggttggt ccaggaattt catttcaatc
      481 tgatgaagac tttgagggtt cgagtgaaag agatatacaa aaggacatta gtcagtttct
      541 aggatatgaa tcagaagatt atagcttagg tgatactttt gatttgtttc attatattca
      601 acaagatgaa acagcttatt atttgaagca aaaagaaaaa gatagaaagg aacaattgaa
      661 ttttctgtta aatattggaa actatgttaa taggaaaaat agattagagc gtttaagaag
      721 tactttatcc tctagtgaaa aggaactaag agcaaaaaag ggtaaactaa aaaattcccc
      781 aattaagaac aatatcctat attcgaaatt atctcttaga gcagatactg actttgcttt
      841 taacaataag tcgattattt ttgatgaaga attgtcaaat gaaagtttaa atatatatct
      901 atcagaaatt actaaattac aagaatttcg aaattcattt tctccaaaag attacttcct
      961 tagaaaacaa agtgagcaat ttgagcgaga ggtgctggat gataaagatt ttactattta
     1021 ctta
//
LOCUS       DAVKDZ010000176          983 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000176 DAVKDZ010000000
VERSION     DAVKDZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.178"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            21..257
                     /locus_tag="VWZ30_002225"
     CDS             21..257
                     /locus_tag="VWZ30_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU9335844.1"
                     /translation="MNNVVTTQISIQLSQELNSLLNAIVEENNTTKTDFIRQAFIEKI
                     EDMYDIKVADEAYKKWVECSKKSISHEEMMRRYG"
     gene            250..981
                     /locus_tag="VWZ30_002226"
     CDS             250..981
                     /locus_tag="VWZ30_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU9335845.1"
                     /translation="MAKLYAISDIHGYLDEFRDALNKVNLNDKDNRLFLLGDYLDNGL
                     QSFQVISKIIELEEVYPNQIITLLGNHEEWFYDWLILDKPTASAFPETIKSFFSPEKL
                     NYIFKSNANNFETDVRNEIKNNIKFNPFINWFKKRYRDERYYETETQIFVHAGIDEEA
                     EKLWKELTSSEIFTNKFLITTGRFHKAIISGHIASWEVAKDRRYLGKIYYDSKSHYFI
                     DGDVTNSKTIPVLYYDTITQKYHFL"
BASE COUNT          374 a          138 c          156 g          315 t
ORIGIN      
        1 aactattagg tggctgttat atgaataatg ttgttactac acaaatttct attcaactta
       61 gtcaagagtt aaattcttta ttaaatgcta ttgttgaaga aaataataca acaaagactg
      121 actttattag acaagctttt atagagaaaa ttgaagatat gtatgatatt aaggtcgctg
      181 atgaagctta taaaaaatgg gttgaatgta gcaaaaaatc tatctcacat gaagaaatga
      241 tgagacgcta tggctaaatt atacgcaata tctgatatac atggatactt ggatgaattc
      301 agagacgctc tcaataaagt aaatttaaac gataaagata atcgcctatt tttactagga
      361 gattatcttg ataatggact tcaatctttt caagtcattt ccaaaataat cgaacttgag
      421 gaggtttatc ctaatcaaat tattactctt ctagggaatc atgaagaatg gttttatgat
      481 tggttgattt tagataaacc tactgcttca gcatttccag aaacaataaa aagcttcttc
      541 tctccagaga aattgaacta tatttttaaa tcgaatgcta acaactttga aacagatgtt
      601 agaaatgaaa ttaaaaataa tatcaaattc aacccattta taaactggtt taaaaaacga
      661 tatagggatg aacgctatta tgaaaccgaa acccaaattt ttgtccatgc tggcattgat
      721 gaagaagcag aaaagctttg gaaagaacta acgagttcag agatatttac aaacaaattt
      781 cttattacca ctggtagatt tcataaagct attatttcgg gccatattgc ttcttgggaa
      841 gtagctaaag atagaagata cctaggtaag atttactatg atagtaaaag tcattacttt
      901 attgatggag atgtaacgaa tagcaaaaca atacctgttc tttactatga tacaattacc
      961 caaaaatatc actttctata act
//
LOCUS       DAVKDZ010000177          956 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000177 DAVKDZ010000000
VERSION     DAVKDZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.179"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..>956
                     /locus_tag="VWZ30_002227"
     CDS             <1..>956
                     /locus_tag="VWZ30_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9335846.1"
                     /translation="QDKVYRNGSDFSVIIVNCLLAVVCKKIDSSSTKLLPEFSGLDFS
                     LWQDYIQSTGSIKELWPSQIELGKQAIFSGKSGIVQMPTSSGKTASINLTLRSAFYSN
                     RIDNALIVAPFRALCREIYRDINAHFVDENNVIVSEVFDLPEIPQDFSIFNDGKKRVF
                     ILTPGKLLFLLRNHQSFIDEIGLCIFDEAHLFDDPSRGTNFELLLSTVKQIFPKGIQK
                     ILISAVIPNSEAINRWFNEDGVIVSNNSIKTTEKRVAFSDLNGSNEQLYFIDPITFEE
                     EFFVPRTVSVSELEQLGKERKQKVFPELTNENDISIYYGINL"
BASE COUNT          341 a          116 c          176 g          323 t
ORIGIN      
        1 tacaagataa agtatatagg aatggaagcg atttttctgt aatcattgta aattgtctct
       61 tagcagttgt atgtaaaaaa atagatagtt catcaacgaa attattacct gaattttcag
      121 gattagattt ttctttatgg caggattata tacaatcaac aggaagtata aaggagcttt
      181 ggccatctca aatcgaatta gggaagcaag caattttttc aggtaaatct ggaatcgttc
      241 agatgccaac aagttcgggt aaaacagcct caatcaatct aaccttaaga tcagcatttt
      301 attcaaatag aattgataat gcattgattg ttgcaccttt tagagcatta tgtagagaaa
      361 tatatcgtga tattaatgca catttcgttg atgagaataa tgtgattgtt agtgaagttt
      421 ttgatttacc ggagattcca caggatttct caatttttaa tgatggtaaa aaaagagttt
      481 ttatacttac tccaggaaaa ttgttgtttt tattgagaaa tcatcaaagt tttattgatg
      541 aaataggact ttgtatattt gatgaagcac atctttttga tgatccaagt cgtggaacaa
      601 attttgaatt actgttgtca acggttaaac aaattttccc aaaaggaata cagaagattt
      661 taatatctgc tgtaatacca aatagtgaag ctataaatcg gtggtttaac gaagatggag
      721 taattgtttc taataattcg attaaaacaa cagagaaaag agttgcgttt agtgatttaa
      781 acgggagtaa tgagcaatta tatttcatag atcctattac atttgaagaa gaattttttg
      841 ttccacgtac tgttagtgtt agtgaattag agcagcttgg aaaagaacgc aaacaaaaag
      901 tttttccaga actgacaaac gaaaatgata taagtattta ttatgggatt aatcta
//
LOCUS       DAVKDZ010000178          900 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000178 DAVKDZ010000000
VERSION     DAVKDZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.180"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            177..>900
                     /locus_tag="VWZ30_002228"
     CDS             177..>900
                     /locus_tag="VWZ30_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001815366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335847.1"
                     /translation="MYPMLVDFINNSQRQYSFINADGISVEDITDEKNLKFYFYGPVK
                     GEIFRYQEKHDLFLYYRDPLTSVEVPVENTIKVVEMYEETDLIIKVGDYIFQDVKRHR
                     FPDGSVELHFGDCFKMSFDVNKKIFKFNYTRSNMLSKAIECTEVLQELGRVGYFNLNG
                     NKIELDKQSISDITSQNLDSDIKDLKQISNILKNIGVQKDLDLNCFDKQSQKNLNILN
                     SGLVLKEKVALDYNESKLLHLRI"
BASE COUNT          339 a          108 c          164 g          289 t
ORIGIN      
        1 ggtggtgtgg ttttattcgt tgtctggcta agtgaaaata atgatttaag agaaatatac
       61 tataagtcat tgcctccttt atctataaaa aaattgctaa aaaaagtaga ttaaataata
      121 aaactacaaa tagtaagaaa ctttctgttc ggatatttaa attggatgag aaagaaatgt
      181 atccaatgtt agtggatttt attaacaata gtcagaggca gtatagtttc ataaatgcag
      241 atggaatttc cgttgaggac attactgatg aaaaaaatct aaagttttac ttttatggtc
      301 cagtgaaagg agagatcttt cgttatcagg aaaagcatga tctttttcta tattacagag
      361 atcctcttac tagtgttgaa gtaccagttg aaaataccat aaaagttgtc gaaatgtacg
      421 aagagactga tcttatcatt aaagttggtg attatatttt tcaagatgta aaaaggcatc
      481 gttttcctga tgggagtgta gaactccatt ttggtgattg ttttaaaatg tcctttgatg
      541 taaataaaaa aatatttaaa tttaactata caaggtccaa tatgctatca aaagctatag
      601 agtgtacgga ggtgctccaa gagctaggaa gagtaggata tttcaatctc aacggaaata
      661 aaatagaact agataagcag tcaatatcgg atatcacttc acagaattta gatagtgata
      721 taaaggattt gaaacagatt tctaacatat taaaaaatat aggtgtacaa aaggatctag
      781 atctaaactg ctttgataaa caatctcaaa aaaatctaaa tatcctaaat tcagggcttg
      841 ttctaaagga gaaggttgcc ttagattata atgagtccaa attacttcat ttaagaattg
//
LOCUS       DAVKDZ010000179          897 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000179 DAVKDZ010000000
VERSION     DAVKDZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.181"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..>897
                     /locus_tag="VWZ30_002229"
     CDS             <1..>897
                     /locus_tag="VWZ30_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU9335848.1"
                     /translation="INDIHAEGYLAVDISPTNFILDDFGRVRLIDLENFTNKNNTVRR
                     AATKFMINTDCPSKICTIQQDYFALAMLSFAIMREHVLIFEGEDVLLNITALDKIKFC
                     MYIEKEIGNITKSQFNWLMYLLNLSEQEKLDRFEKMKYINDFEDKNIEFHLKKVDSSF
                     NLQIENETLINYLLSLNMNRESRILNSNEFGEFVSPISFQHGLSGYLFYLSHMRDISE
                     NGRIIEWIISEGNQNKGNVYLNGCSILFGSSGYLWVLLEFYKKITSNNPSRELIIDKI
                     RDIYKDLISSYQKVEVFDFALG"
BASE COUNT          329 a           98 c          158 g          312 t
ORIGIN      
        1 tattaatgat attcatgctg agggatattt agcagtggat atttctccaa caaactttat
       61 attggatgat tttggtcgtg ttaggttaat agacttagaa aattttacaa acaaaaacaa
      121 tactgttaga agagctgcta caaaattcat gataaatact gattgtccct caaaaatctg
      181 taccattcaa caggattatt ttgcacttgc aatgttgagt tttgctatta tgcgcgagca
      241 tgttttaata tttgaaggag aggacgtctt attaaatata actgctttag ataaaatcaa
      301 gttttgtatg tatattgaga aagaaattgg gaacataact aaatcgcaat ttaattggtt
      361 gatgtattta ttgaatttaa gtgaacaaga gaagttagac cgattcgaga agatgaagta
      421 tataaacgat tttgaagata aaaatataga atttcattta aagaaagttg attcctcatt
      481 caatcttcaa attgaaaatg aaacgcttat taactatctt ctttcgttaa atatgaatag
      541 agaaagcagg atactaaatt caaatgaatt tggagaattt gttagtccaa tatcgtttca
      601 acatggctta tcaggatatt tgttttattt atcacatatg agagacattt ctgagaatgg
      661 tagaattatc gagtggatta tctctgaagg aaatcaaaat aaaggaaatg tgtatctaaa
      721 tggttgttca attttatttg gttcttccgg atatctttgg gtattattag agttctataa
      781 aaaaattacc agcaacaatc caagtagaga attgatcata gataaaatta gagatattta
      841 taaagacttg ataagtagtt atcaaaaagt tgaagtattt gactttgctt taggaaa
//
LOCUS       DAVKDZ010000180          890 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000180 DAVKDZ010000000
VERSION     DAVKDZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.182"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..584
                     /locus_tag="VWZ30_002230"
     CDS             <1..584
                     /locus_tag="VWZ30_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9335849.1"
                     /translation="QREGDTHHSYKHIAPVYAIAIVDSNYFQDDLAFHSFSMREDTTG
                     EVLTITTNGQENHLVKMAFLELKKYRETSKDEVRKPWLEFFGNKPFTQEPERAISQAD
                     QLLDYKSWSEEDRKMFSQLRMREEQALLAHDYSLEQAEEKGLERGRAEGLERGLERGK
                     LFAFLDMVRQGLLTSEVASQQLGMTVAEFEALL"
BASE COUNT          281 a          170 c          192 g          247 t
ORIGIN      
        1 gccaacgaga aggtgataca caccatagtt acaaacatat cgcaccagtt tatgctattg
       61 ccatcgtaga tagtaattac ttccaagatg atttggcctt tcacagtttt agtatgcgcg
      121 aagacacgac aggtgaggtc ttaactatca caactaacgg tcaagaaaac catttggtca
      181 agatggcatt cttggaacta aaaaaataca gagaaaccag caaagacgag gttcgtaagc
      241 cgtggttgga gtttttcggg aacaaaccct ttacccaaga acccgagcga gccatcagcc
      301 aagcagacca actgctggac tacaagagct ggtccgagga ggacagaaaa atgtttagtc
      361 aactacgtat gcgagaagaa caggcattat tggctcatga ctattccttg gaacaagctg
      421 aggaaaaagg gttggagaga ggtcgtgcag aaggacttga acgtggtctt gaacgtggaa
      481 aactttttgc ctttctggat atggttcgcc aaggacttct gacttccgag gttgccagtc
      541 agcagttggg catgaccgtt gctgagtttg aggcgttgtt gtaagatcag caataataga
      601 caggaaaacc atctggtcaa gatggcgttc ttggaattaa aaaactctag gtataaaatc
      661 ttaaaaatta caaacacgca tagtatcagg ggattaagaa accttgatac tatgcgtttt
      721 atagtgcatt gaatctacat tagtacacta tagttgattg aaaacagaat agtataccgc
      781 agcttctaaa tccttgttat aaatttgatt tgactttcct aatcgattta ttctcatctt
      841 atttcaatct gctatattta cttcactttc tcctaaaatt gactttcttg
//
LOCUS       DAVKDZ010000181          763 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000181 DAVKDZ010000000
VERSION     DAVKDZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.183"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            185..>763
                     /locus_tag="VWZ30_002231"
     CDS             185..>763
                     /locus_tag="VWZ30_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU9335850.1"
                     /translation="MSSSKTSSVSYFYFAFDVLGILIFVGKAYNFINPKGIKMAKQVF
                     QTTFAGRELIVETGQLAKQANGSVVVRYGESTVLTAAVMSKKMATGDFFPLQVNYEEK
                     MYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDENA
                     SAPMAAMFGSSLALSISDIPFDGPIAGVQVGYV"
BASE COUNT          198 a          150 c          173 g          242 t
ORIGIN      
        1 cttggtatct tgatgacttg aattttggtc tcatttctta ggaaaaaaga taagcttcct
       61 agcactcgtc gagtgcgacg tcaccttcct atttttctac agaaatgttc ggcaagccga
      121 accgtccaaa atatcttgtg taattgaaca cggccgaaaa gctgtgtaaa aaagataaac
      181 tgtcttgtct tcatcgaaga cttcgtcagt ttcctatttt tactttgctt ttgacgtcct
      241 tggtatcttg atctttgtag gcaaggcgta taatttcatc aatccaaagg ggattaaaat
      301 ggcaaaacaa gtgtttcaaa cgacttttgc gggtcgtgag ttaattgtag agactggtca
      361 gcttgctaag caagcaaatg gctctgttgt tgtacgttac ggtgagtcaa ctgtcttgac
      421 tgctgccgtt atgtctaaga aaatggcaac tggggatttc ttcccactcc aagtcaacta
      481 cgaagaaaaa atgtatgcgg ctgggaagtt tcctggtggc tttatgaaac gtgaaggacg
      541 tccttcaaca gatgcgacct tgacagcgcg tttgattgac cgtccgattc gtcctatgtt
      601 tgcggaaggt ttccgtaatg aagtccaagt catcaataca gtgctttctt atgatgaaaa
      661 tgcatctgca ccaatggctg ctatgtttgg ttcatctttg gcactgtcta tttcagatat
      721 tccatttgac ggaccaattg ctggggtaca agtgggatat gta
//
LOCUS       DAVKDZ010000182          528 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000182 DAVKDZ010000000
VERSION     DAVKDZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.184"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..>528
                     /locus_tag="VWZ30_002232"
     CDS             <1..>528
                     /locus_tag="VWZ30_002232"
                     /inference="COORDINATES: protein motif:HMM:NF019192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEU9335851.1"
                     /translation="KLKAAKDLLVPKSDNTGLTTAKNALDTERNKAVDTTGKTPASVA
                     AYNDAKQKAQEASDAAQTVLNNPNATEQQIQDEITKVNAAKEKLGKAEAGLTTAATAQ
                     AKQELTTAKEGLEEPVSTDGMTPDSIKAYEAAKKEAAQAVADAKAAAQAAEAKGENAT
                     EAEVNEAKAKVDAAK"
BASE COUNT          241 a           98 c          125 g           64 t
ORIGIN      
        1 aaaaattaaa agcagctaaa gatctgttag taccgaagtc agacaataca ggtttaacaa
       61 cagctaagaa tgcattagat acagagcgta ataaggctgt agatacaaca ggtaagacac
      121 cagcgtcagt agcagcgtac aatgatgcaa aacaaaaagc gcaagaagca tcagacgcag
      181 cgcaaacagt gttaaacaat ccaaacgcaa cagaacaaca aatccaagat gaaattacga
      241 aagtaaatgc agcgaaagag aagttaggaa aagcagaagc aggattaaca acagcagcaa
      301 cagcgcaagc aaaacaagag ttaacaactg caaaagaagg attggaagag ccagtatcaa
      361 cagacggaat gacaccggac agtataaaag catacgaagc agctaagaaa gaagcagcgc
      421 aagcagtagc agatgcaaaa gcagcagcgc aagcagcaga agctaaaggt gaaaacgcga
      481 cagaagcaga agtaaatgaa gcgaaagcga aagtagacgc agctaaag
//
LOCUS       DAVKDZ010000183          520 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000183 DAVKDZ010000000
VERSION     DAVKDZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.185"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..>520
                     /locus_tag="VWZ30_002233"
     CDS             <1..>520
                     /locus_tag="VWZ30_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9335852.1"
                     /translation="VKESTKHNCKSVDKKMKEVIPSDTILANLDRRFLQEAIEKIIES
                     NGYITAKKVRHRLRGIFNYAVQYSYIENNEVDYTTIPQKPKTLEELEKKRNNFLTMQE
                     IKALVDVLNRREYHQKYADMVLVLTLTGMRYGELTALQLKNIDFENNKIEITGNFDSV
                     NKIKTLPKTTNSI"
BASE COUNT          205 a           92 c           89 g          134 t
ORIGIN      
        1 gtaaaggaat caacaaaaca taattgtaaa tcagttgata agaagatgaa ggaagtcata
       61 ccatccgata ccatacttgc taatcttgac aggcgttttc ttcaagaggc tattgaaaaa
      121 attattgaaa gcaacggata tattacagct aaaaaagtac ggcataggct cagaggtatc
      181 tttaattacg ctgttcaata ctcttacatt gaaaacaacg aggtcgatta tactacgatt
      241 cctcaaaaac caaagacttt agaagaactg gaaaaaaagc gtaacaactt tctcaccatg
      301 caagaaataa aagcacttgt cgatgtcctt aatcgtcgag aatatcacca aaagtacgct
      361 gatatggttc ttgtgctgac attaactggt atgagatatg gtgagttaac tgccttacaa
      421 ctgaagaata tagacttcga aaacaacaaa attgagatca caggtaattt tgattcagta
      481 aacaaaatca agacgctacc aaagactaca aattcaatac
//
LOCUS       DAVKDZ010000184          472 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000184 DAVKDZ010000000
VERSION     DAVKDZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.186"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..>472
                     /locus_tag="VWZ30_002234"
     CDS             <1..>472
                     /locus_tag="VWZ30_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU9335853.1"
                     /translation="SLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELI
                     AFQEEIVAAVGKEQAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQVVK
                     DQVTAVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDE"
BASE COUNT          156 a           88 c          122 g          106 t
ORIGIN      
        1 aatctcttct tgaattgaca gtagctggaa ccaagcacgc tatcaacatg gtagagtctg
       61 gtgccaaaga attgtcagaa gaaatcatgt tggaagcgct ccttaaaggg cacgaagctg
      121 tcaaagaatt gattgccttc caagaagaaa tcgttgctgc tgtcggtaaa gaacaagcag
      181 aagtggaatt gcttcacgtg gatgctgaat tgcaagctga aatcattgca gcctacaaca
      241 gtgacctcca aaaggcagtt caagtagaag agaaattggc ccgtgaagct gcgactcaag
      301 tagtgaaaga ccaagtgact gccgtttacg aagaaaaata tgcggaccac gaagaatttg
      361 accgtattat gcgtgatgtg gctgaaatct tggaacaaat ggaacacgca gaagtgcgac
      421 gtttaattac agaagacaag gtgcgtcctg atggtcgtaa ggtcgatgaa at
//
LOCUS       DAVKDZ010000185          404 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000185 DAVKDZ010000000
VERSION     DAVKDZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.187"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..>404
                     /locus_tag="VWZ30_002235"
     CDS             <1..>404
                     /locus_tag="VWZ30_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEU9335854.1"
                     /translation="TRLLLKASDKAPWSDNGDAKNPALSPLGENVKTKGQYFYQVALD
                     GNVAGKEKQALIDQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTININGLISK
                     ETVQKAVADNVKDSIDVPAAYLEKAKGEGPFTA"
BASE COUNT          147 a           94 c           79 g           84 t
ORIGIN      
        1 actcgtctct tactcaaggc atctgacaag gcaccatggt cagataacgg tgacgctaaa
       61 aatccagccc tatctccact aggcgaaaac gtgaagacca aaggtcaata cttctatcaa
      121 gtagccttag acggaaatgt agctggcaaa gaaaaacaag cgctcattga ccagttccga
      181 gcaaacggta ctcaaactta cagcgctaca gtcaatgtct atggtaacaa agacggtaaa
      241 ccagacttgg acaacatcgt agcaactaaa aaagtcacta ttaacataaa cggtttaatt
      301 tctaaagaaa cagttcaaaa agccgttgca gacaacgtta aagacagtat cgatgttcca
      361 gcagcctatc ttgaaaaagc caagggtgaa ggtccattca cagc
//
LOCUS       DAVKDZ010000186          401 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000186 DAVKDZ010000000
VERSION     DAVKDZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.denovo.188"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            complement(<1..>401)
                     /locus_tag="VWZ30_002236"
     CDS             complement(<1..>401)
                     /locus_tag="VWZ30_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9335855.1"
                     /translation="ETGFDTYFYREYGRSLKGQLRRGKVSGRRYQRISLVAGLTNGEL
                     IAPMTYEEMMTSDFFEAWFQKFLLPTLNTPSVIIMDNARFHRMGKLELLCEEFGHKLL
                     PLPPYSPEYNPIEKTWAHIKKHLKKVLPSCNT"
BASE COUNT          116 a           80 c           71 g          134 t
ORIGIN      
        1 gtattgcaac ttggtaatac ctttttgagg tgctttttga tatgagccca tgttttctca
       61 ataggattgt actcaggtga gtagggagga agaggtaaaa gtttatgccc aaactcttcg
      121 cataaaagtt ctagcttccc cattctatgg aatcttgcat tatccataat aataaccgat
      181 ggtgtgttta atgttggtaa gagaaatttc tgaaaccaag cttcaaaaaa gtcgctcgtc
      241 atcatctctt cgtaagtcat tggagcgatt aactcaccat ttgttagacc tgcaaccaaa
      301 gaaatcctct gatatcttct tccagatact ttgcctcttc ttaactgacc ttttaatgag
      361 cgaccatatt ctcgataaaa ataagtatcg aatcctgttt c
//
LOCUS       DAVKDZ010000187        22490 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.guided.025, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000187 DAVKDZ010000000
VERSION     DAVKDZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22490
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.guided.025"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            <1..1044
                     /locus_tag="VWZ30_002237"
     CDS             <1..1044
                     /locus_tag="VWZ30_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9335856.1"
                     /translation="FVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWKETLP
                     EISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGERFPIL
                     RKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGYVEQS
                     ISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRSIFKI
                     VDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGLKFAF
                     RSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVFEELL
                     HAFDTRPPVRKEV"
     gene            1047..3224
                     /locus_tag="VWZ30_002238"
     CDS             1047..3224
                     /locus_tag="VWZ30_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU9335857.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            3221..4222
                     /locus_tag="VWZ30_002239"
     CDS             3221..4222
                     /locus_tag="VWZ30_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEU9335858.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            4219..5151
                     /locus_tag="VWZ30_002240"
     CDS             4219..5151
                     /locus_tag="VWZ30_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU9335859.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5396..5512
                     /locus_tag="VWZ30_002241"
     CDS             5396..5512
                     /locus_tag="VWZ30_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEU9335860.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5528..7447
                     /gene="tet(M)"
                     /locus_tag="VWZ30_002242"
     CDS             5528..7447
                     /gene="tet(M)"
                     /locus_tag="VWZ30_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEU9335861.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7566..7733
                     /locus_tag="VWZ30_002243"
     CDS             7566..7733
                     /locus_tag="VWZ30_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEU9335862.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(7793..8146)
                     /locus_tag="VWZ30_002244"
     CDS             complement(7793..8146)
                     /locus_tag="VWZ30_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9335863.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            8651..9073
                     /locus_tag="VWZ30_002245"
     CDS             8651..9073
                     /locus_tag="VWZ30_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9335864.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            9070..9300
                     /locus_tag="VWZ30_002246"
     CDS             9070..9300
                     /locus_tag="VWZ30_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9335865.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            9761..9964
                     /locus_tag="VWZ30_002247"
     CDS             9761..9964
                     /locus_tag="VWZ30_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEU9335866.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            10046..11263
                     /locus_tag="VWZ30_002248"
     CDS             10046..11263
                     /locus_tag="VWZ30_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9335867.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            11533..12123
                     /locus_tag="VWZ30_002249"
     CDS             11533..12123
                     /locus_tag="VWZ30_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEU9335868.1"
                     /translation="MVDKREKLMNSFNQYGFLTFKQVIDENLHYKTLLKMVAEGKIDA
                     EEKGLYRLPDIYLDEWFVLQYRFPKGIFSLETALWLHGLSLTIPFNMTMSFPYGTNTK
                     NIKEADICPIILRSHYSEGIIEIERLPGQFIKVYEVERVLVECLRPVHQVDLQIIAPA
                     FKKYFQQNKIHLHKLFYYAQLFKVTDKLQSYTEVLS"
     gene            12123..12959
                     /locus_tag="VWZ30_002250"
     CDS             12123..12959
                     /locus_tag="VWZ30_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEU9335869.1"
                     /translation="MFSNANSFKAKIKNISKDKGIPAQQVQQHYLIEQVLKLISTSSY
                     RDSFIVKGGYLIGQMIGLDKRTTMDLDVTLKGTEMSRENLIHIFEEILCSNTDGFSFS
                     VDKLEPIRQDDEYGGFSLKLNATFDTLKEVVFIDITTGDKITPREITYSMTSIFTNES
                     IKIWTYNLETVLAEKLETIISRGLASTRPRDRYDLFTLYKLRKEEINLEVLKNALENT
                     AEKRKSKDTIYNWEEQVRGIEISDYQKELWIRYQRQFKYAKDISFDNSVQVIREIMQQ
                     IF"
     gene            13242..13541
                     /locus_tag="VWZ30_002251"
     CDS             13242..13541
                     /locus_tag="VWZ30_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335870.1"
                     /translation="MKVVNLYDLKQMGNKGGCTIQLIHHFPFGMGLGHLKKDYIEFKR
                     VGIVDGKAVEVTLREPYSRDLLQVVKSIKQRQKLIAYRYKEGKLLFVKKEASDVL"
     gene            13531..14019
                     /locus_tag="VWZ30_002252"
     CDS             13531..14019
                     /locus_tag="VWZ30_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335871.1"
                     /translation="MSSEQQERMAVQYAERSLLFTVKSLLKILEWSRRQALAQDSAYK
                     IGVQKLEELLQSPYSIDTINLKKDFLDKPIDIEKFKAFLEKEEIPLAIAWQGDSLHFY
                     TKDRSILDNHLDHLLEKMVNDPEKLADFTMDKSLDDAIDEAKSQITFRQEGAVKQKEM
                     VR"
     gene            14019..15896
                     /locus_tag="VWZ30_002253"
     CDS             14019..15896
                     /locus_tag="VWZ30_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEU9335872.1"
                     /translation="MYSGKKFLLFSLLGILLGYLFHRLTLLYDSYTGNTLDKWTHLLM
                     EGQDEVLQSPWNVSFTGKSSAFFLLGFVMMLLVYLYLETGKKQYREGVEYGSARFGTL
                     KEKKLFYGKEFSHDTILAQDVRLTLLDKKPPQYDRNKNIAVIGGSGSGKTFRFVKPNL
                     IQMNSSNIVVDPKDHLAEKTGKLFLEHGYQVKVLDLVNMKNSDGFNPFRYIETENDLN
                     RMLTVYFNNTKGSGSRSDPFWDEASMTLVRALASYLVDFYNPPKTREQLIEESRLSQK
                     EYQNLLKRQKKEVEERKKRGRYPSFAEISKLIKHLSKGENQEKSVLEILFENYAKKYG
                     TENFTMRNWADFQNYKDKTLDSVIAVTTAKFALFNIQSVMDLTKRDTLDMKTWGQEKS
                     MVYLVIPDNDSTFRFLSALFFSTVFQTLTRQADIDFKGQLPLHVRVYLDEFANIGEIP
                     DFAEQTSTVRSRNMSLVPILQNIAQLQGLYKEKEAWKTILGNCDSLVYLGGNDEDTFK
                     FMSGLLGKQTIDVRNTSRSFGQTGSGSLSHQKIARDLMTPDEVGNMKRHECLVRIANM
                     PVFKSKKYNSTKHPNWKYLANQETDERWWNYQINPLNQRQENHLEGLRIRDLTFESSL
                     K"
     gene            15917..16159
                     /locus_tag="VWZ30_002254"
     CDS             15917..16159
                     /locus_tag="VWZ30_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335873.1"
                     /translation="MITHFKGFVYGVDASAMFAQAMSLLQKGLIAVGAFLVVVGIVNL
                     ATNIKDGGPGVRNAILEIVGGVMVGAAGAFVTQISI"
     gene            16176..17030
                     /locus_tag="VWZ30_002255"
     CDS             16176..17030
                     /locus_tag="VWZ30_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL"
                     /protein_id="HEU9335874.1"
                     /translation="MNLSLVSPFVYLASEKISAENLFEGFNVDLQSTVDLIKSLSSYN
                     PTVWTYMSSITKSVMQPLGVAILSVVLILEFSKMAKKIANSGGAMTFEALAPMLISYI
                     MVAVVITNTTVIVEAIIGIASHAIEQVASIVAHGGAKYDTISGLKGSGFIGRMIVGFF
                     ALLIWLVRIVSAAMVNLLVSIRFIQLYLMIPFAPLTIPTFLSDEWKSIGIGYLKNIMV
                     YAVQGVLIFLIVSLVPLFESAGKIAVSNGAGVLQSLAIMFGSLVQAILLIIALVGSQR
                     TDRSILGM"
     gene            17084..17443
                     /locus_tag="VWZ30_002256"
     CDS             17084..17443
                     /locus_tag="VWZ30_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEU9335875.1"
                     /translation="MNTRVFKDISKYQHRAWLGFTTRQIIFVLPAFIVTIIVLGLNLF
                     FWQFGDWFVYGFVFAFTIPLMLFGVYKPNDLYFEHYLKYRLHFELTVPLRTITGKKGP
                     EHEKKIKYIKETKNFND"
     gene            17436..19751
                     /locus_tag="VWZ30_002257"
                     /pseudo
     CDS             17436..19751
                     /locus_tag="VWZ30_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            19763..22468
                     /locus_tag="VWZ30_002258"
     CDS             19763..22468
                     /locus_tag="VWZ30_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU9335876.1"
                     /translation="MKDKREIIRARKAFRRSLKDEKKFLKQGKKEVRKQKKDSAVLDE
                     KAWKKEIKEKLEEMREASKERVKQANEDYNHILQNSPPSFLNRKELRDRRLPHARKRL
                     KIAKKQFREAKVEAKEERKESRKERKTNQKFLYGQESKQKSNFFFQGKSLEELKAKKE
                     VKAAKENLKSTKQAYKYKKVSRKAKTFLYVLGREGGELASENEDLEGYRTLQETIRKG
                     KRYSRLSYNLGKASVKTGQATGRFTKKRLTNTKERYHHFKDGKGWKLAKDNPSSFKNR
                     FRKLKKQGLSSVQNIYQKLKAAFSFFTFAAGNPATWIVGGIVFLLLLMMSFFLGFSSA
                     SLIQQDEFELTKAYTHLTWEDAEHTRTNDKGITYYTKVDDVMGYMNFKFHDYELQKPV
                     HLFSSETYKDYLSTLWHDLNDGDDLKSMQDLYETPKYKLSKDDQEEIKELKEEGVYAS
                     MQELDNPFEGQSNEDSLTMTYRYGYYDLDGKPTLQEYILLEAKAHQTIVAPMDAVVSL
                     DGDNIILTNGKGENESRLTLYSIHNGRAIEGTRVLTGDIIGETPDDTGLKVSYQKYKN
                     KKEKLVYVNPQFYFPKVIQLQTTILPAIGQFGGDEFERAKHIYDFLKSQGVSPQAIAA
                     ILGNWSVESSINPKRAEGDYLSPPVGATDSSWDDESWLAIGGPAIYSGAYPNILHRGL
                     GLGQWTDTSDGSTRYTALLNYAHSKNKKWYDLDLQLDFMLHGDSPYYQSWLKDFFKNT
                     GSAANLAQLFLTYWEGNSGDKLLERQTRATEWYYQNEKGFSQTNGGQAKSDPQSLEGV
                     RGDLYDHSVPGGGDGMAYAYGQCTWGVAARMNQLGLKLKGSNGEKISIINTMGNGQDW
                     VATASSLGGETGSTPRAGAIVSFVGGTHGTPADYGHVAFVGARRF"
BASE COUNT         7515 a         3821 c         4608 g         6546 t
ORIGIN      
        1 tttgttggtt cgctgggtgg tggtaaatcc tttgcgaata accttatcgt ctattatgcg
       61 gtgctttatg gggcacaagc agtgattgta gacccaaaag cagaacgtgg cagatggaaa
      121 gaaaccttgc cagagatttc ccatgaaatc aatatcgtca ctctgacttc tgatgagaaa
      181 aacaaaggct tacttgaccc ttatgtgatt atgaaaaatc ccaaagattc tgaatcactg
      241 gctattgata ttctgacatt ccttacgggg atttcctctc gtgatgggga acgcttccca
      301 atccttagaa aagccattcg tgcagtaacc aatagtgaag tacgagggtt gatgaaagtg
      361 attgaggaat tacgggttga gaatacgcca ctaagtacca gtatagccga ccatatcgaa
      421 agttttacag actatgactt tgcacattta ttattcagta atggttatgt ggagcagtct
      481 atcagcttag aaaaacaact gaacattata caggttgcgg acttggtact tcccgacaag
      541 gaaacttcct ttgaggaata taccactatg gagcttttat ccgttgctat gctgattgtc
      601 attagtacct ttgctttaga ctttatccat acagaccgaa gcattttcaa gattgtagat
      661 ttagacgaag catggagctt tttacaggta gcacaaggaa aaacactatc tatgaagctg
      721 gttcgggctg gtcgtgctat gaacgctggg gtatatttcg tgacccaaaa tacagacgac
      781 ctcttagatg aaaaactgaa aaataacctc ggcttaaaat ttgcatttcg ttccactgac
      841 cttaacgaga ttaaaaagac cttagccttt tttggtgtag acccagagga cgaaaacaat
      901 cagaagcgat tgcgtgattt ggaaaacggg caatgcctta tcagtgattt atatggtcgt
      961 gtcggtgtga tacagttcca ccctgtattt gaagaactgc tccatgcctt tgataccaga
     1021 ccacctgtgc gaaaagaggt gtaaatgtga aaccatcaat agtaaacaga ataaaatcaa
     1081 actggacgct gaaacgtcta ggtaaagtgg caatgacagt ggctttcaca cttgtgattg
     1141 ccatttttct tttagccatg ctgggaacgg tggttcaagc tgcgggcttg gtagatgata
     1201 cggtcaatgt ggcaaatgaa tacagccgat acccacttga aaactatcaa ctggattttt
     1261 atgtggataa tagctggggc tggcttccgt ggaactggtc ggacgggatt ggaaaacagg
     1321 tcatgtatgg actatatgcc attaccaatt ttatttggac aatcagtttg tatgtttcca
     1381 atgcgacagg ttacttagta caggaagcct attccttaga cttcatttcc gctacagcag
     1441 attccattgg taagaatatg cagaccttag ctggtgtgag tgcaaacgga ttttcaacag
     1501 agggtttcta tgttggattc ctcttactct tgattttggt tcttggggtt tatgttgcct
     1561 atacgggact gataaagaga gaaaccacaa aggcaattca tgccattatg aattttgtgc
     1621 tggtgtttat cctatcggct tcctttattg cctacgctcc cgactacatt aaaaaaatca
     1681 atgacttttc atcagacatc agtaatgcca gtttatcact tggcacgaag attgtcatgc
     1741 cccattccga tagtcaaggc aaggacagcg tggacttaat cagagatagc ctgttttcca
     1801 tacaggttca gcaaccgtgg ctactgcttc aatacaacag ttcagacatt gaaagtatcg
     1861 gtattgaccg tgtggaaagc ctgctctcca ccagcccaga ttccaacaat ggcgaagaca
     1921 gagaaaaaat tgttgcggaa gaaattgaag acagaagcaa taccaatcta accattacaa
     1981 agaccattaa ccgtttaggt acagtcttct tcctatttgt cttcaatatt gggatttcca
     2041 tatttgtatt cctattaaca ggaatcatga ttttctcgca ggtacttttt atcatctatg
     2101 ctatgtttct gcctgtgagc tttattttaa gcatgattcc atcatttgat ggtatgtcaa
     2161 aacgagccat aacaaagctc tttaatacca ttttgacacg agctggaatc acattgatta
     2221 ttacgacagc atttagtatt tcaaccatgc tctatacctt atcggctggt tatccgttct
     2281 ttttgattgc ttttctacag attgtgacct ttgcaggaat ctacttcaag ctgggcgatt
     2341 taatgagtat gttttctcta cagagtaacg attctcaaag tgtgggaagt cgtgtgatga
     2401 gaaaacctcg tatgcttatg cacgctcaca tgcaccgtct acagcggaaa cttggacgtt
     2461 ccatgactac tctaggggct gggtctgcca ttgttacagg taaaaaagga cagtcgggtt
     2521 cggggagttc tgcaaggaca caagcagatc actcccgacc agacggaaag gaaaaatcaa
     2581 cacttggaaa acgtatcggt caaaccatcg gtacagtagc tgataccaaa gacagaatgg
     2641 tagacactgc tagtggtttg aaagaacagg ttaaagattt gccgaccaat gcaagatatg
     2701 cagtatatca aggaaaatcc aaagtaaaag agaatgtccg tgatttaacc agtagtattt
     2761 ctcaaaccaa agcggacaga gccagtggac gcaaggaaca gcaggaacaa aggcgaaaaa
     2821 ccattgcgaa gcgtcgctct gaaatggaac aggtcaaaca gaaaaaacag cctgcttctt
     2881 ctgttcatga aagaccgact acaagacaag aacaatatca tgatgaacag acctcaaaac
     2941 agtctaatat tcagacttca tataaggaat ctcaacaagc caaacaagag cgtccagcag
     3001 ttaagtccga tttttcaagt ccaaaagtgg aacgccaagg caataccgtt caagaaaaaa
     3061 ccgttcaaaa gccagcaact tcaaccacta cagcagatag aacttcacaa cgtccaatca
     3121 caaaagaacg tccgtctact gttcaaagag taccactaca aaatacaaga agtagaccac
     3181 caatcaaaac cgccaccatt aagaaagtcg gtaagaaacc atgaagttga aaactttagt
     3241 gattggtggt tctggattat tcttgatggt cttctcactg cttctgtttg ttgccatttt
     3301 attttcagat gaacaggaca gcggaatttc caatattcat tatggaggtg tgaatgtttc
     3361 cgcagaagtg ctggctcata agcctatggt agaaaaatat gccaaagaat atggcgttga
     3421 agaatatgtc aacatacttc ttgcgattat acaggtggaa tcgggcggta ctgcggaaga
     3481 tgttatgcag tcctcggaat ccctcggtct tccacctaat tcattgagta cagaagaatc
     3541 cattaagcaa ggtgtgaagt atttcagtga attattagcc agtagcgaaa ggctcagtgt
     3601 agatttagaa tcggttatcc agtcctacaa ttatggtggt ggtttcttag ggtatgtggc
     3661 taatcgtgga aataaatata cctttgaact ggctcaaagt ttctcaaaag agtattcagg
     3721 tggcgaaaaa gtgtcttacc ccaatcccat agccatacct atcaatgggg gctggcgata
     3781 caactatggc aatatgtttt atgtgcaact ggtaacgcag tatcttgtca caacagagtt
     3841 tgatgatgat acggtacaag ccatcatgga cgaagcactg aaatatgagg gctggcgata
     3901 cgtttacggt ggagcttccc cgactacttc ttttgattgt agcggactga cacaatggac
     3961 gtatggaaaa gctggaatta acttaccacg aaccgcacaa cagcaatatg atgtgaccca
     4021 gcatatccca ctatcggaag cacaagctgg cgatttggtt ttctttcatt ctacctataa
     4081 cgctggctct tatattactc atgttgggat ataccttggc aataaccgta tgtttcatgc
     4141 aggcgaccca atcggttatg ccgacttaac aagcccctac tggcaacagc atttagtggg
     4201 agcaggacga atcaaacaat gagaaaggaa gatttaatga tgaaatttag aaaaaatcag
     4261 aataaagaaa aacagatacc aaaggaaaag aaacctcgtg tctataaggt caatcctcat
     4321 aaaaaggttg tgattgcctt gtgggtactt ttagggctta gtttcagctt tgcgatattc
     4381 aagcacttta cagctataga tactcatact attcacgaaa caactatcat agaaaaggaa
     4441 tacgttgata ctcatcatgt agaaaatttt gtagagaact ttgcgaaagt ctactattca
     4501 tgggagcaat ccgataagtc cattgataat cgaatggaaa gtctaaaagg ctatctgaca
     4561 gatgaacttc aagctctcaa tgttgataca gtacgcaaag atattcctgt atcgtcttct
     4621 gtaagaggat ttcagatatg gacggtagag ccaactggcg acaatgagtt taatgtaacc
     4681 tacagtgtag accagctcat tacagaggga gaaaatacaa agaccgtcca ctctgcttat
     4741 atagtgagtg tctatgtaga tggttctgga aatatggtac tggttaagaa tccgaccatt
     4801 accaacatac ctaagaaatc aagttataaa ccaaaagcca ttgaaagtga ggggacggtt
     4861 gattccatta caaccaatga aatcaatgag tttttaacga cgttcttcaa gctctatcct
     4921 acagcgacag ccagtgaact ttcctactat gtgaatgacg ggatattaaa accaatcgga
     4981 aaagagtaca tctttcaaga actggtaaat cctattcaca atcgtaagga taatcaagtc
     5041 acggtatcgc tgacagtgga gtatatcgac cagcagacca aagcaacgca ggtatctcaa
     5101 tttgatttgg tacttgaaaa gaacgggagt aattggaaga ttatagaata acaaatattg
     5161 gtacattatt acagctattt tgtaatcacg tactctcttt gataaaaaat tggagattcc
     5221 ttgacaaata tgctcttacg tgctattatt taagtatcta tttaaaagga gttaataaat
     5281 atgcggcaag gtattcttaa ataaactgtc aatttgatag tgggaacaaa taattggatg
     5341 tcctttttta ggagggctta gttttttgta cccagtttaa gaataccttt atcatgtgat
     5401 tctaaagtat ccggagaata tctgtatgct ttgtatgcct atggttatgc ataaaaatcc
     5461 cagtgataaa agtatttatc actgggattt ttatgccctt ttgggttttt gaatggagga
     5521 aaatcacatg aaaattatta atattggagt tttagctcat gttgatgcag gaaaaactac
     5581 cttaacagaa agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa
     5641 aggtacaacg aggacggata atacgctttt agaacgtcag agaggaatta caattcagac
     5701 aggaataacc tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca
     5761 tatggatttc ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact
     5821 gatttctgca aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa
     5881 aatggggatt cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc
     5941 aacggtttat caggatatta aagagaaact ttctgccgaa attgtaatca aacagaaggt
     6001 agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt
     6061 aatagaggga aacgatgacc ttttagagaa atatatgtcc ggtaaatcat tagaagcatt
     6121 ggaactcgaa caagaggaaa gcataagatt tcataattgt tccctgttcc ctgtttatca
     6181 cggaagtgca aaaaacaata tagggattga taaccttata gaagtgatta cgaataaatt
     6241 ttattcatca acacatcgag gtcagtctga actttgcgga aaagttttca aaattgagta
     6301 ttcggaaaaa agacaacgtc ttgcatatat acgtctttat agtggcgtac tgcatttgcg
     6361 agattcggtt agagtatcag aaaaagaaaa aataaaagtt acagaaatgt atacttcaat
     6421 aaatggtgaa ttatgtaaga ttgatagagc ttattctgga gaaattgtta ttttgcaaaa
     6481 tgagtttttg aagttaaata gtgttcttgg agatacaaaa ctattgccac agagaaaaaa
     6541 gattgaaaat ccgcaccctc tactacaaac aactgttgaa ccgagtaaac ctgaacagag
     6601 agaaatgttg cttgatgccc ttttggaaat ctcagatagt gatccgcttc tacgatatta
     6661 cgtggattct acgacacatg aaattatact ttctttctta gggaaagtac aaatggaagt
     6721 gattagtgca ctgttgcaag aaaagtatca tgtggagata gaactaaaag agcctacagt
     6781 catttatatg gagagaccgt taaaaaatgc agaatatacc attcacatcg aagtgccgcc
     6841 aaatcctttc tgggcttcca ttggtttatc tgtatcaccg cttccgttgg gaagtggaat
     6901 gcagtatgag agctcggttt ctcttggata cttaaatcaa tcatttcaaa atgcagttat
     6961 ggaagggata cgctatggtt gcgaacaagg attatatggt tggaatgtga cggattgtaa
     7021 aatctgtttt aagtacggtt tatactatag ccctgttagt accccagcag attttcggat
     7081 gcttgctcct attgtattgg aacaagtctt aaaaaaagct ggaacagaat tgttagagcc
     7141 atatcttagt tttaaaattt atgcgccaca ggaatatctt tcacgagcat acaacgatgc
     7201 tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa aataatgagg tcattcttag
     7261 tggagaaatc cctgctcggt gtattcaaga atatcgtagt gatttaactt tctttacaaa
     7321 tggacgtagt gtttgtttaa cagagttaaa agggtaccat gttactaccg gtgaacctgt
     7381 ttgccagccc cgtcgtccaa atagtcggat agataaagta cgatatatgt tcaataaaat
     7441 aacttagtgt attttatgtt gttatataaa tatggtttct tgttaaataa gatgaaatat
     7501 tttttaataa agatttgaat taaagtgtaa aggaggagat agttattata aactacaagt
     7561 ggatattgtg tcctgtatgt ggaaataaaa cacgattaaa gataagggaa gatactgaat
     7621 taaaaaaatt ccccctctat tgtccgaaat gcagacaaga aaatttaatt gaaataaagc
     7681 agttcaaagt aactgtgatt acagagccag acgcaaagac gcagagccga taaaatgaga
     7741 ttaatacaat ctcattttat cggctctttc cgttatgtat ggattctttt aattagtctt
     7801 cgatgtttct tgcttcgttg ataccgctgg ctaaagattc cattaaggat agttctttgt
     7861 ctgtaaagct atccatgtat ttctctatct gtaatcgtcg ggtgcttttt accaagttat
     7921 tagcaggtaa gaaaaattca tcaacggaaa catgaagtaa cgatacaagg tcataaagaa
     7981 cttgtatgct ggggtgttgc cctttatttt caatattagt taagtaccgt gggtcaattt
     8041 caatcaatgc tcccacttgt tcacgagtta aacctcgttt caatcgagct tctttaatgg
     8101 ctaaaccaaa ggctctaaaa tcatatttat cttctttttt acgcatagta gaccacctct
     8161 atacatttta ttgttcctac tgaattaaaa acaggtatag aaaaacgtgt tatatggttt
     8221 ataggtttat atttaataaa aagcactact aaacgccaat aaaaaaaacc gttatatggt
     8281 agtgctattt acgctgttaa aatattgtat attacttcca aatggcggtt tgttggaggt
     8341 caacgtcgcc atgaagtaca tcatatacaa taaatttcct tacattgggt tcttgtcaaa
     8401 aaaagtcgtc tatctgcaat agataagtac gtccaccaat gtggttttat aaatcatata
     8461 gatagaataa cagaagcatg taaacagaga aataaatctg tttatatgct tttttggcta
     8521 ttcagaactt ttttacaaag tttatttatc agtaatgcaa caaatccccc tttcacattg
     8581 ggactaagag tgaaaggaga taaacgagca aggctcactt cctttcctag acagaaaggg
     8641 ggtgagaaac atgaaaccat cttcttttca gaccacaata gaaaatcagt ttgactatat
     8701 ctgtaaacgt gctatggaag acgagcgaaa gaattatatg ctttatcttt caaggattgc
     8761 aaagcgtgag gtgtcctttt cggatgttgg cgattatctt gttagccagt ttgcgacaac
     8821 agataactat tcaactgact ttcagatttt tacactcaat gggttatcag taggcgttga
     8881 aaatgatttg ttgagtgaag cattacgtga gttgccagac aagaaacgtg aaattctact
     8941 gctgttttac tttatggaca tgagcgattc agaaattgca gacctgttga aattgaaccg
     9001 ttctactgtc tatcggcata gaaccagtgg actagcctta attaaaaagt ttatggagga
     9061 atttgaagaa tgaaaacaca atatcctatg attccctttc ctctcattgt aaaggcaaca
     9121 gatggcgata ccgaagcgat taaccagatt ctacatcatt acagagggta cataacgaag
     9181 cgttccctac gacttatgaa agatgaatat ggcaatcaaa gtatggtcgt tgatgaagtc
     9241 ttacgtggaa gaatggaaac cagactgatt acaaagattt tgtcatttga aattaagtaa
     9301 tatcctctct cctttcgtgg aagcgtgcta aaccattcca cgcttcccga acagggaggt
     9361 ttgttattcc accaaagcat attgagcttt caatgtgttt tgataggcta acgagccatt
     9421 gttctttgaa aactgaataa aagtaatcga atacgtttcg ataagaaaag agccaacgga
     9481 actaaccgcc atgacctatc ttataaagat agcgagcgat tcatgttagt gatccgagaa
     9541 gcaatcttta gcaggattgc ctgcaacgac attcttatcg tgataatgat actcccatac
     9601 agtcaatagt ccgagcgtga taaaaccgtc gcaggcaatg agtatggcta catgagaacc
     9661 atgcaggggt ggaactcccg tgagctttgc taaagctgtt cgattgctgg taaaacaact
     9721 tttatgaaat ccaaataagt gatttggaaa ggaggatttt atgaagcaga ctgacattcc
     9781 tatttgggaa cgttataccc taaccattga agaagcgtca aaatattttc gtattggcga
     9841 aaacaagcta cgacgcttgg cagaggaaaa taaaaatgca aattggctga ttatgaatgg
     9901 caatcgtatt cagattaaac gaaaacaatt tgaaaaaatt atagatacat tggacgcaat
     9961 ctagcgtcgc caaagggtct tgtatatgat aaaatagtat taagtcgtat caaggctctt
    10021 tccataaagg aaaggagcaa atgccatgtc agaaaaaaga cgtgacaata aaggtcgaat
    10081 cttaaagact ggagagagcc aacgaaaaga cggaagatac ttatacaaat atatagattc
    10141 atttggagaa ccgcaatttg tttactcgtg gaaacttgtg gctacagacc gagtaccagc
    10201 aggaaagcgt gattgtatct cacttagaga gaaaatcgca gagttacaga aagacattca
    10261 tgatggtatt gatgttgtag gaaagaaaat gacactctgc cagctttacg caaaacagaa
    10321 cgctcaaaga ccaaaggtta gaaaaaacac tgaaactgga cgcaaatatc ttatggatat
    10381 tttgaagaaa gacaagttag gtgtaagaag tattgacagt attaagccat cagacgctaa
    10441 agaatgggct attagaatga gtgaaaatgg ttatgcttat caaaccatca ataactacaa
    10501 acgttcttta aaggcttcat tctatattgc tatacaagat gattgtgttc ggaagaatcc
    10561 atttgacttt caactgaaag cagttcttga tgatgatact gtccctaaga ccgtactaac
    10621 agaagaacag gaagaaaaac tgttagcctt tgcaaaagct gataaaacct acagcaaaaa
    10681 ttatgatgaa attctgatac tcttaaaaac aggtcttcgt atttcagagt ttggtggttt
    10741 gacacttcca gatttagatt ttgagaatcg tcttgtcaat atagaccatc agctattgag
    10801 agatactgaa attgggtact acattgaaac accaaagacc aaaagtggcg aacgtcaagt
    10861 tcctatggtt gaagaagcct atcaagcatt taagcgagtg ttagcgaatc gaaagaatga
    10921 taagcgtgtt gagattgatg gatatagtga tttcctcttt cttaatagaa agaactatcc
    10981 aaaagtggca agtgattaca acggcatgat gaaaggtctt gttaagaaat acaataagta
    11041 taacgaggat aaattgccac acatcactcc acatagtttg cgacatacat tctgtaccaa
    11101 ctatgcaaat gcaggaatga atccaaaggc attacagtac attatgggac atgctaatat
    11161 agccatgacg ctgaactatt acgcacatgc aacattcgat tctgcaatgg cagaaatgaa
    11221 acgcttgaat aaagagaagc aacaggagcg tcttgttgct tagtagtaca aatgaattta
    11281 ctacttattt accacttctg acagctaaga catgaggaaa tatgcaaaga aacgtgaagt
    11341 atcttcctac agtaaaaata ctcgaaagca catagaataa ggctttacga gcatttaaga
    11401 aaatataaaa agataattag aaatttatac tttgtttatt tataaaaaaa ctaaatgaat
    11461 tgattttaaa gaaataaaat gctaacatat atagtagatg ttagtatttt atttctaaga
    11521 aaggagaatg atatggttga taaaagagag aaactgatga actctttcaa tcagtatggt
    11581 tttttaactt ttaaacaagt aatagatgaa aatttacact acaaaacctt attaaaaatg
    11641 gttgcagaag gaaaaatcga tgctgaagaa aaagggttat atcgcttacc tgatatttat
    11701 ttagatgagt ggtttgtcct tcagtatcga tttccaaagg gaatcttttc tttggagaca
    11761 gcactttggt tacatggttt atctttgact atccctttta acatgacgat gagttttcct
    11821 tatggtacga ataccaaaaa cattaaggaa gcagatatat gtcctattat cttacgctct
    11881 cactatagtg aaggaattat tgaaatagag cgccttcctg gtcaatttat taaagtttat
    11941 gaagttgagc gagttttggt tgaatgtcta agaccagttc atcaggtgga tcttcaaatt
    12001 attgcaccag cgtttaaaaa atattttcaa caaaataaaa ttcatttaca caaattattt
    12061 tattatgccc agctatttaa agtaactgat aagttacaat cttatacgga ggtactatct
    12121 taatgttttc aaatgcgaat agctttaaag caaaaatcaa aaacatttca aaagataagg
    12181 gaattccagc tcaacaagta caacaacatt atttaattga gcaagtgtta aagctgattt
    12241 ctactagttc ttatagagat tccttcattg taaaaggagg gtatctaata ggtcaaatga
    12301 ttggactaga taagcgaacc acaatggatt tagatgtcac tctgaaggga accgaaatga
    12361 gcagagaaaa tttgattcat atctttgaag agattctttg ttctaatact gatggatttt
    12421 cattttcagt agataagcta gaacctattc gtcaagatga tgaatatgga ggattttcat
    12481 taaaattaaa tgcaactttt gatacattaa aagaggttgt ttttattgat attactactg
    12541 gtgataaaat cacaccaaga gaaattactt attcaatgac ttctatcttt actaatgaaa
    12601 gcatcaagat atggacatat aatctagaga ctgtacttgc tgaaaagtta gaaacaatca
    12661 tcagtagggg actagcttcg acgcgtccac gtgatcgata tgatcttttc accttgtata
    12721 aacttagaaa agaagagatt aatcttgaag tattaaaaaa tgcacttgag aatacggcag
    12781 aaaaacgtaa aagtaaagat actatttata attgggaaga acaagtgaga ggaattgaaa
    12841 tttctgatta tcagaaagag ctatggattc gttatcaacg ccaatttaag tatgctaaag
    12901 atatctcatt tgataactct gttcaggtca ttagggaaat tatgcaacaa atattttagt
    12961 acaaaagtaa ctcattagta aagcagtgag ttgctttttt tattccaata actccaaaaa
    13021 tgtgataaaa atttcaacta ctcttatcta taataaagct acctgttctt tgaaaattga
    13081 atccactccg tcttgattag cgtgcctgtg taagtaggga ctgagagtat ttccctgtga
    13141 agggcaactg gcacaagacg tgtgtgagaa ttttctaaca gacacggagt ttatcgttac
    13201 cagacttaaa cgatgcgaca aactagataa aggagttaat catgaaggta gtaaacttgt
    13261 atgatttgaa acaaatggga aataaaggtg gttgtaccat ccaattgatt catcactttc
    13321 cttttggtat gggcttagga catctcaaaa aagactacat tgaatttaaa cgtgttggta
    13381 tcgttgatgg gaaggcagta gaagtcactc ttcgagaacc ttattcgaga gatctactgc
    13441 aggttgtcaa gtcgattaag caacgtcaga aattgatagc ttatcgttat aaagaaggaa
    13501 agctgctatt tgtgaagaag gaggcatcag atgtcctctg aacaacagga acgaatggca
    13561 gttcaatatg cggagcgtag tcttttattt actgtcaaaa gtctcttaaa aattctagaa
    13621 tggtctagac gtcaggcttt agcacaggat tccgcctata agataggggt gcagaaatta
    13681 gaagaattgc tacaatctcc ttattcgatt gatacgatta atctgaaaaa agatttttta
    13741 gacaaaccaa ttgatataga gaaatttaaa gcttttttag aaaaagaaga gattccttta
    13801 gccatcgctt ggcaagggga ttctctacat ttctacacga aagaccgttc gattctggac
    13861 aatcatttag accatctgtt agaaaaaatg gttaatgatc cggagaaatt agctgatttt
    13921 accatggata agtcattaga cgatgcaatt gatgaggcga aatcccaaat tacctttaga
    13981 caagaagggg ccgtcaaaca gaaagagatg gtgagatgat gtacagtgga aagaaattcc
    14041 tactattttc actgttaggt atcttactag gctatctttt tcatcgtttg acgcttttgt
    14101 atgattccta tactggaaat acattagata aatggactca tcttctgatg gaaggtcaag
    14161 atgaagttct tcagtcgcca tggaatgttt cctttactgg aaaatccagt gctttttttc
    14221 tactaggctt tgtgatgatg ttgctggttt atctttattt agagactggc aaaaaacaat
    14281 accgagaagg ggtagaatac gggagcgccc gttttggaac tctaaaagaa aagaagctct
    14341 tttacggtaa ggaattttct catgatacga tcttagctca agatgttcgt ttgacattat
    14401 tagataaaaa accaccccaa tatgatagaa ataagaatat tgcggtgatc ggaggttcag
    14461 gaagtgggaa gacatttcgc tttgtgaaac ctaatcttat tcagatgaat agttctaata
    14521 ttgtagtgga tcctaaagat cacttggccg aaaaaacagg caaactcttt ttagaacatg
    14581 gctatcaagt aaaggtgcta gatttagtca atatgaagaa ctcagatggc ttcaatcctt
    14641 ttcgctatat agagacagaa aatgatttga atcgcatgct gacggtttat tttaataaca
    14701 ccaaaggctc tggctcccga agtgacccat tttgggatga agcttctatg actttggttc
    14761 gagctttagc ctcttacttg gtcgatttct ataatccacc caaaacaaga gaacagctca
    14821 ttgaagagag tcgtttgagt caaaaagaat accagaactt gttgaaacgt caaaaaaaag
    14881 aagtggaaga gcgaaaaaaa cgaggacgtt atccaagttt tgctgaaatc tcaaaactca
    14941 ttaaacactt atctaagggt gaaaaccaag aaaaaagtgt cttagaaatt ctatttgaaa
    15001 attatgctaa aaagtatggc actgaaaatt ttaccatgcg aaattgggca gatttccaaa
    15061 attataagga taagactctg gattctgtta tagctgtaac cactgctaaa tttgccctct
    15121 tcaatattca aagtgtcatg gatttgacca aaagagatac ccttgatatg aagacatggg
    15181 gccaggaaaa atcaatggtt tacttagtta tcccagataa cgatagtacc tttcgctttc
    15241 tttcagccct ctttttttca accgtatttc aaaccctaac aagacaagca gatattgatt
    15301 ttaagggtca attgcctctt catgtgagag tctacttaga tgagttcgca aatataggag
    15361 aaatcccaga ttttgctgaa caaacctcaa cagtccgttc tcgaaatatg agtctcgttc
    15421 ccattcttca aaatattgcc caacttcaag ggctctataa agaaaaagaa gcttggaaaa
    15481 ccattttggg gaactgtgat agcttagtct acttaggtgg gaatgatgaa gataccttta
    15541 aatttatgag tggcttactc ggtaaacaaa ccattgatgt tcgaaatact agtcgttcct
    15601 ttggccagac aggttcagga tccctttctc atcaaaagat tgctcgtgat ttaatgacac
    15661 ctgatgaggt cggaaatatg aaacggcatg aatgcttggt tcgaattgcc aatatgcctg
    15721 tctttaaaag caaaaaatac aattccacta agcatccaaa ctggaaatac ctagccaatc
    15781 aagaaaccga tgaacggtgg tggaactatc aaatcaatcc tttgaatcaa agacaagaaa
    15841 atcatcttga aggccttaga attcgtgatt taacttttga atctagttta aaataaaaat
    15901 agaaaagagg aaatagatga ttacgcattt taaaggtttt gtttatggag tagacgcaag
    15961 tgctatgttt gcacaagcta tgtctttgtt acagaagggt ttgattgcgg ttggtgcctt
    16021 tctcgttgtt gtggggattg tcaatcttgc aaccaacatt aaagatggtg gaccaggtgt
    16081 tcggaatgcc attctggaaa ttgtcggtgg tgttatggta ggggctgctg gagcctttgt
    16141 aactcaaatt tcaatttagg aggataaaca atgacatgaa tcttagttta gtctcaccct
    16201 ttgtttacct tgcatctgaa aaaatatcag ctgaaaattt atttgaaggg tttaatgtag
    16261 atttacaatc tacggtagat ctgattaaat ccctatctag ctacaatcca acagtttgga
    16321 cttatatgtc tagtattact aaaagtgtca tgcagcctct tggagttgcg attttatcag
    16381 ttgttctcat cttagaattt tcgaagatgg caaagaaaat tgctaactca ggaggagcga
    16441 tgacctttga agcattagcg ccgatgttga ttagttatat catggtcgca gtcgtgatta
    16501 ccaatactac cgttattgta gaagccatca tcgggattgc gagtcacgcc attgaacaag
    16561 tggcctcaat tgtggctcac ggtggggcaa agtatgatac catctctgga ttaaaaggtt
    16621 caggatttat tggccggatg attgtgggct ttttcgccct cctcatttgg ctagttcgga
    16681 tagtaagtgc agccatggtt aaccttttgg tatctattcg atttattcaa ctctacctta
    16741 tgattccatt tgcccctctt acgattccaa catttttaag tgatgaatgg aagtctattg
    16801 gtattggcta tttaaaaaat attatggttt atgcggtaca aggggttctc atttttctga
    16861 ttgtttctct tgttccttta tttgaatctg ctgggaaaat agctgtttca aatggtgcag
    16921 gagtcttgca atcacttgcg attatgtttg gtagtttggt acaagctatc ttactgatta
    16981 ttgccctcgt tggttctcaa cgtacggatc gctcaatctt aggtatgtaa ttagataaag
    17041 gctaggaagt gattgcttct tagccttttt agaaaggaaa gtcatgaata cacgtgtctt
    17101 taaagacatc tcaaaatacc aacacagggc ttggttaggt ttcaccacaa gacaaatcat
    17161 ctttgtttta ccagccttta ttgtcacaat tattgttttg ggcttgaatc tctttttctg
    17221 gcaatttgga gattggtttg tttacggttt tgtgtttgct tttaccatcc ccctcatgct
    17281 ttttggagtc tataaaccca atgatttata ttttgaacat tatttgaaat accgtcttca
    17341 ttttgaacta acggttcccc tacgcacaat tacaggaaag aaaggacctg aacatgaaaa
    17401 gaaaatcaaa tacattaaag aaacaaaaaa cttcaatgac taatcagaaa gaagtagtta
    17461 aaggaaaaaa agaggaagtg ttaccatcga cggctaatac tctttcctat caagccctgt
    17521 atcaaaatgg tctgatgcag gtaaaagaag attatttctc acaaagctat ttacttggtg
    17581 atgtcaatta ccagactgtt ggtttagaag ataagggcgc aatcattgag aagtattctg
    17641 atttgattaa ctccctagac gaccaaacca acttccaatt gactatcttt aataaaagat
    17701 tgaatttaga aaagtttaga caaagtgttt tgtatgagga aaaagaagat gggtatgata
    17761 gctatcgtaa agaattgaat cggatgatga atcagaattt agacagtggt gaaaataact
    17821 tttcagctgt gaaactgatt agctttggta gaaaggattc taatcccaaa caagcctatc
    17881 gttccttgtc tcaaattgga gaatatttca agagtggttt ctcagaaatt gatgctcgat
    17941 ttgaatcttt ggctggagaa gaacgggtga acttgctggc agatatgctt agaggagaac
    18001 accatcttcc tttttcttac cgtgatttaa cgagatctgg tcagacaact cgtcacttca
    18061 tagcacctaa tctcttggat tttaaaaaca agaattacct acaaatcaat gaccgcttat
    18121 tgcagattgt ctatgtcaga gactacggta tggaattagg agatcagttt atttgagacc
    18181 tcatgcaagg agatttggaa ttgattgtga gcctccatgc tcaaagttcg accaaggcgg
    18241 atgctatgaa gaaactacga acaaagaaga ccttgatgga atcccaaaag attggggaac
    18301 aacaaaaact tgctcgtaca ggtatctatt tggaaaaagt aggtcatgta ttagaaagca
    18361 atatcgatga agctgaggaa ctcttaaaaa ccatgaccga gacaggagat aaactatttc
    18421 aaacagtatt cttgattggt gtttttggtc aggatgaaga agaacttaaa caagctctag
    18481 atacgattca acaagtggcc ggctcaaatg acctaatgat tgataaacta ccatatatgc
    18541 aagaagcagc ctttaatagt ttgctgccat ttggttgtga ttttttagag ggagtatcac
    18601 ggagtttatt aacatccaat atagcagtga actctccatg gacttcagta gacttacaag
    18661 accgtagtgg aaaatattac ggtatcaatc aaatctcaag caatattatt accattgatc
    18721 gcagcctatt aaatacaccg tctggtctga ttttagggac atctggagct gggaaaggga
    18781 tggcaaccaa gcatgaaatt atcacgaccc aaatcaagga atctggtgaa aatactgaaa
    18841 ttatcatcgt ggatccagaa gcagaataca gtgtcattgg acgagctttt ggtggagaaa
    18901 tgattgatat tgcgcccgat tcccaaactt atctcaatgt ccttgatttg tctgaggaaa
    18961 atatggatga agatcctgta aaggtaaaat cagaatttct tttatccttt atcggcaagt
    19021 tattggatag aaaaatggat ggaagagaaa aatcgattat cgaccgagtc acaagactca
    19081 cctatcagtc atttaaagag ccttctttgg aagaatgggt ctttgtcttg agccaacaac
    19141 cagaagaaga agcgcagaat ttggcacttg atatggaact gtatgtcgaa ggttctcttg
    19201 atattttttc tcataagacc aatattcaga caggatctaa tttcttgatt tataacgtta
    19261 agaagttagg agatgagctg aaacaaatcg cccttatggt tgtttttgat cagatatgga
    19321 atcgtgtcgt tcggaatcaa aaattaggga agaagacctg gatttatttt gatgaaatgc
    19381 agcttctctt attagataaa tatgccagtg atttcttctt taaattgtgg agtcgtgtca
    19441 gaaaatatgg agccagtctg actggaataa ctcaaaacgt cgaaacctta ttgttagatc
    19501 caaatggtag acggattatt gcaaatagtg aatttatgat tcttctcaag caagcaaaaa
    19561 atgaccgaga agaactggtt caactcttag gcttgtcaaa agaactcgaa aaatacctag
    19621 tcaatccaga aaaaggagca ggactgataa aagctggttc agttgtcgta ccatttaaaa
    19681 ataagattcc tcaaggtact caattgtttg atattatgag tacggatcct gataaaatgg
    19741 cttctaatta aggggaaggt aaatgaagga taaaagagaa atcatacgtg cccgaaaagc
    19801 atttagaaga agtctaaaag atgagaagaa attcttgaaa caaggaaaga aggaggtgag
    19861 gaaacagaaa aaagattccg ctgtactgga tgaaaaagca tggaaaaaag agataaaaga
    19921 aaagctagag gagatgagag aagcttcaaa ggagagagta aaacaagcaa atgaagacta
    19981 caatcatatt cttcaaaata gtcctccatc ttttttgaat cgtaaagaat taagagacag
    20041 acgattacct catgctagga aacgattgaa aatagccaag aagcaattta gagaagccaa
    20101 ggtagaagca aaagaagaaa gaaaagagag tcgtaaagaa agaaaaacca atcaaaaatt
    20161 tctctacggt caggaatcga aacaaaaatc taattttttc tttcaaggga agagtttaga
    20221 agaattaaaa gctaagaaag aagtcaaggc tgctaaagaa aatttaaaat ctactaaaca
    20281 agcctataag tacaaaaaag tcagtaggaa agccaaaact tttctttatg tccttggacg
    20341 tgaaggtgga gagttagctt cagaaaatga agatttagaa ggctatcgca cacttcaaga
    20401 gaccattaga aaagggaaac gctacagtcg gctttcttat aatcttggaa aagctagtgt
    20461 caaaacagga caagcaacag gtcgttttac caagaaaaga ctgaccaaca caaaagagcg
    20521 ataccatcat tttaaggatg gaaaaggatg gaaactagca aaagataacc caagttcctt
    20581 taagaatcgg tttcgaaaat taaagaaaca aggtctttca agtgtccaaa atatctatca
    20641 aaaactaaaa gcagcctttt ccttctttac atttgcggct ggaaatcctg caacatggat
    20701 agttggagga atagtctttc ttcttttact tatgatgagc ttcttcttag gattttcatc
    20761 tgctagtttg attcaacaag atgaatttga attaacaaaa gcttataccc acctaacttg
    20821 ggaagatgca gaacatactc gcacaaatga caaaggaatt acctattaca caaaagttga
    20881 tgatgtgatg gggtatatga actttaaatt ccatgactat gagttacaga aaccagttca
    20941 cttatttagt tcagaaactt acaaggatta tctgtctact ttgtggcatg atttaaacga
    21001 tggtgatgat ttgaaatcca tgcaagacct ctatgaaact cctaagtata aactatcgaa
    21061 agacgatcaa gaggaaataa aggaactaaa agaagaaggt gtgtatgctt ccatgcagga
    21121 attagacaat ccatttgagg gacaaagcaa cgaagatagt ctaaccatga cttatcgtta
    21181 tggatactat gatttagatg ggaaacctac tcttcaggag tacatcctac tagaagcaaa
    21241 ggctcaccaa acgattgtcg caccaatgga tgcagttgta tctctagatg gtgacaatat
    21301 tattctcact aacggaaaag gagagaatga gagtcgatta accttgtatt ctattcataa
    21361 tggccgtgcg attgagggga caagagtcct aacgggtgat attattggtg aaacaccaga
    21421 cgatacaggt ttgaaagttt cctatcaaaa gtataagaac aagaaagaaa aattggtgta
    21481 tgtcaatccg caattttatt ttccaaaagt cattcaactt cagaccacta tcttacctgc
    21541 cattggtcag tttggtgggg atgagtttga acgagcaaaa catatttatg attttttgaa
    21601 atctcaaggc gtaagtcccc aagccattgc ggctatttta ggaaattggt ctgtagagtc
    21661 ttctattaat ccaaaacgag ctgaaggaga ttatttatct cctccagttg gcgctaccga
    21721 ttcctcatgg gatgatgaaa gctggttagc gattggaggt ccagccattt atagtggtgc
    21781 ttatcctaat attcttcata gaggtttggg gttagggcaa tggacggata cttcagatgg
    21841 ttcaacacgt tatacagctt tattgaatta tgcacatagc aaaaataaga agtggtatga
    21901 tttagacctc caacttgatt ttatgcttca tggggatagt ccttactacc aaagttggtt
    21961 aaaagatttc tttaaaaata cgggcagtgc agccaatctc gcccaactct tcctcaccta
    22021 ttgggaggga aattctggtg acaaattact ggaaagacaa accagagcaa cggaatggta
    22081 ttaccaaaat gaaaaaggct tcagccaaac aaatggagga caggcaaaaa gtgacccaca
    22141 atcccttgaa ggggttcgtg gggacttgta tgatcattct gttcctggtg gtggagatgg
    22201 tatggcctat gcctatggac aatgtacatg gggtgttgcg gctcgtatga atcaattagg
    22261 cttaaaatta aaaggaagta atggagagaa gatttcaatc attaatacca tgggaaatgg
    22321 tcaggactgg gttgcgacag cttcaagtct tggtggggaa actggctcta caccaagagc
    22381 aggtgctatt gtttcttttg tgggaggtac acatggcaca ccagcagact atggtcatgt
    22441 agcttttgta ggtgcgagac gtttctaact gaaaaggtta gacacgttct
//
LOCUS       DAVKDZ010000188         5353 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074022
            SAMN33606169-rid17951103.guided.115, whole genome shotgun sequence.
ACCESSION   DAVKDZ010000188 DAVKDZ010000000
VERSION     DAVKDZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606169
            Sequence Read Archive: SRR23727560
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722602.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722602.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 03:24:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,265
            CDSs (total)                      :: 2,215
            Genes (coding)                    :: 2,053
            CDSs (with protein)               :: 2,053
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 79 of 162
            Pseudo Genes (incomplete)         :: 67 of 162
            Pseudo Genes (internal stop)      :: 51 of 162
            Pseudo Genes (multiple problems)  :: 29 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606169-rid17951103.guided.115"
                     /strain="AUSMDU00074022"
                     /serotype="alternative_aliB_NT"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4149"
     gene            11..94
                     /locus_tag="VWZ30_002259"
     CDS             11..94
                     /locus_tag="VWZ30_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEU9335877.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            219..956
                     /gene="erm(B)"
                     /locus_tag="VWZ30_002260"
     CDS             219..956
                     /gene="erm(B)"
                     /locus_tag="VWZ30_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEU9335878.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            complement(1213..2460)
                     /locus_tag="VWZ30_002261"
     CDS             complement(1213..2460)
                     /locus_tag="VWZ30_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9335879.1"
                     /translation="MSHNDSILNILGIKDKNIKIISVEEAEHNNDSVKEYITLITATL
                     SYPINRCRNCGFPTVNKDGFRKTHVRLASLNGRRYELELRKQRYKCKSCHTTFGAITN
                     LTKENQTLSSDLKNQIMLLARKGLSGQLIAEMCHCSPSSVRRTILERMEPHYRVAKLP
                     KHLCFDEFRSIKSVMSFICCDAETHQIVTKLQDRLSPTIVDYFESRYSKAERECVQSV
                     VIDLNAQYQSFIYRLFPNANIIIDRFHLVQLAGRALDNCRISILKQLDKQSQEYKIMK
                     SHWKLFHKKAEDLHPEEVVFLRGVKQYMTRQNAVDLITSKFSKFAEVYQTYQDITKAL
                     NERNSELLESTILDYQKTNTEMDTAIQTLRQNRKYVLNSAKFEYSNGPLEGINRKIKT
                     LKRTCYGFANQKFFFLRIDCIFS"
     gene            2640..2861
                     /locus_tag="VWZ30_002262"
     CDS             2640..2861
                     /locus_tag="VWZ30_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9335880.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            2978..3475
                     /locus_tag="VWZ30_002263"
     CDS             2978..3475
                     /locus_tag="VWZ30_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEU9335881.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            3450..3956
                     /locus_tag="VWZ30_002264"
     CDS             3450..3956
                     /locus_tag="VWZ30_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU9335882.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            3940..>5353
                     /locus_tag="VWZ30_002265"
     CDS             3940..>5353
                     /locus_tag="VWZ30_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEU9335883.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVG"
BASE COUNT         1720 a          932 c         1058 g         1643 t
ORIGIN      
        1 gggattcgtc atgttggtat tccaaatgcg taatgtagat aaaacatcta ctgttttgaa
       61 acagactaaa aacagtgatt acgcagataa ataaatacgt tagattaatt cctaccagtg
      121 actaatctta tgacttttta aacagataac taaaattaca aacaaatcgt ttaacttctg
      181 tatttgttta tagatgtaat cacttcagga gagattacat gaacaaaaat ataaaatatt
      241 ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa
      301 aagaaaccga taccgtttac gaaattggaa caggtaaagg gcatttaacg acgaaactgg
      361 ctaaaataag taaacaggta acgtctattg aattagacag tcatctattc aacttatcgt
      421 cagaaaaatt aaaactgaat actcgtgtca ctttaattca ccaagatatt ctacagtttc
      481 aattccctaa caaacagagg tataaaattg ttgggaatat tccttaccat ttaagcacac
      541 aaattattaa aaaagtggtt tttgaaagcc gtgcgtctga catctatctg attgttgaag
      601 aaggattcta caagcgtacc ttggatattc accgaacact agggttgctc ttgcacactc
      661 aagtctcgat tcagcaattg cttaagctgc cagcggaatg ctttcatcct aaaccaaaag
      721 taaacagtgt cttaataaaa cttacccgcc ataccacaga tgttccagat aaatattgga
      781 agctatatac gtactttgtt tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta
      841 aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa caatttaagt accattactt
      901 atgagcaagt attgtctatt tttaatagtt atctattatt taacgggagg aaataattct
      961 atgagtcgct tttttaaatt tggaaagtta cacgttacta aagggaatgg agataaatta
     1021 ttagatatac tactgacagc ttccaagaag ctaaagaggt ccctagcgcc tacggggaat
     1081 ttgtatcgat aaggggtaca aattcccact aagcgctcgg gaccccttgt aggaaaatgt
     1141 cctaagtggg atatctgtca actggtatgg ttgactaaaa atacttccta cgaaaatgta
     1201 gggggtattt ttttacgaaa aaatacaatc gattcttaaa aagaaaaatt tttgattggc
     1261 aaaaccataa caagttcgtt ttagggtttt gattttgcga ttgatgcctt ctaaaggacc
     1321 attagagtat tcaaatttag cgctatttaa gacatatttt ctgttttgac gaagggtttg
     1381 aatagcagta tccatttctg tattggtttt ttggtagtct aagatggttg actctagtaa
     1441 ttcactattg cgctcgttta gggctttcgt gatatcttgg taagtttggt atacttcagc
     1501 gaacttggaa aatttactag taatgagatc aacagcattt tggcgagtca tatattgttt
     1561 aacgccgcga agaaaaacta cttcttcagg gtgaagatct tcagcttttt tatggaatag
     1621 cttccaatgt gacttcataa ttttatattc ttggctctgt ttatcaagtt gctttaggat
     1681 agagatacga caattgtcca aagcgcgacc agctaattgt acaaggtgga agcgatcaat
     1741 aatgatattg gcattaggga aaaggcgata gataaaactt tgatattgag catttaaatc
     1801 aattacaact gattgaacgc attcgcgttc ggcttttgaa taacgacttt caaaataatc
     1861 aacaatggta ggtgatagac gatcctgtaa ctttgtgaca atttggtggg tttcagcgtc
     1921 acaacagata aaggacatca cagacttaat tgaacgaaac tcgtcaaaac atagatgctt
     1981 aggcaactta gccacacgat agtgtggttc catgcgctct aagattgttc gacgaacact
     2041 gctaggagag cagtgacaca tttcagcaat aagctgacca gataagcctt tacgagctaa
     2101 aagcatgatt tgatttttga gatcactgga taaggtttga ttttctttgg ttaaattagt
     2161 aatagcacca aaagtagtat ggcatgattt acatttatag cgttgtttac gaagctctag
     2221 ttcatatctt ctcccattta aacttgccag tcgtacatga gttttgcgaa agccatcctt
     2281 attaactgtg ggaaagccac agttacgaca acgattaatc ggataagaaa gagtagctgt
     2341 tattagcgtt atatactctt taacagaatc gttgttgtgt tcagcttctt caacagaaat
     2401 aattttaata tttttatctt taattccaag aatatttagg atagaatcat tatgggacat
     2461 ttgtttaacc ttctttcatg atttttgtgg tgaattgatt gtataacgag gggacagcaa
     2521 atgtcctctt ttttgtataa aaaaatctgg catggaatct ctatccatac cagaaagtgt
     2581 ataccccaaa aaaataactc aaatacaaat tcattgaata tagagaggag aacattttta
     2641 tgaattttgg acaaaacctt tataactggt ttctatcaaa cgctcaatca ctggtgcttt
     2701 tagcaatcgt tgtgattggc ttgtatcttg gcttcaagcg tgagtttagc aaactgattg
     2761 gctttttaat tattgcgatt attgcggttg gcttagtctt caacgctgct ggagtaaaag
     2821 acattttact agagctattc aatcgcatta ttggtgctta aataaaaccg ttcttttgtg
     2881 gaatataagt ggttttctta tgttccgcaa aggaatggta caccaaacga agtgcggtag
     2941 ggatttttga atctctacaa agaaaggacg tgaatatatg gacgatatgc aagtctatat
     3001 tgcgaattta ggcaaataca atgagggcga attggtcggt gcgtggttta cctttcccat
     3061 tgactttgag gaagtcaaag agaaaatcgg cttgaatgat gaatatgagg aatacgccat
     3121 tcatgactac gagttaccct ttacggttga cgaatacact tccattggcg aactcaatcg
     3181 actatgggaa atggtatcgg aattacccga agaattacaa tcggagctat ctgctctgct
     3241 cactcatttt tcaagcattg aagaactaag cgaacatcaa gaggatatta tcattcattc
     3301 cgattgtgat gatatgtatg acgtggcacg ctactacatt gaagaaacgg gtgctttagg
     3361 cgaagtacca gctagtcttc aaaactatat tgattatcaa gcctatggtc gggatttaga
     3421 cctttcagga acgtttatct caaccaatca tgggattttt gaaatcgtct attaaatctg
     3481 tcggtacatt actactggca gattttctat tttacggggt ggctcaatca gctaccccta
     3541 ttttttatga aaggattgat tacatgaaga aaatacgaag ctataccagt atctggtctg
     3601 tggaaaaggt actgtattct atcaatgatt ttagacttcc gtttcccata acctttacgc
     3661 aaatgacatg gtttgtcgtg tcactctttg cagtgatgat acttggcaac ttgccccctc
     3721 tttccatgat agagggagca tttctcaaat actttgggat tcctgtggct ttcacatggt
     3781 ttatgtctac aaaaactttt gatggtaaaa agccttatgg atttttgaag tctgtcattg
     3841 cttatgcact gcgaccaaag ctgacctatg caggaaaaaa agtaacgctt ggcagaaacc
     3901 agccacaaga agccattaca gcagttagga gtgaatttta tggcatatcc aattaaatac
     3961 attgaaaaca atctcgtctg gaataaagac ggggaatgtt atgcttacta tgagcttgtt
     4021 ccttacaatt actcatttct cagtccagaa cagaaaatac aagtgcatga ttctttcaga
     4081 cagcttatcg cacaaaatcg tgatggcaaa attcatgctt tacaaatcag tacagaatcc
     4141 agcatacgtt ctgcacaaga gcgttccaaa aatgaagtca ctggcaagct caaagcggtt
     4201 gcctatgaca aaatcgacca acagacagac gctttaatat ccatgattgg cgaaaatcaa
     4261 gtgaactacc gtttctttat cggctttaag ttgcttctca acgatcagga gttttctatg
     4321 aaaagtctta ccgttgaagc aaaaaatgct ttgtctgatt ttgtctatga tgtgaaccat
     4381 aagctgatgg gcgattttgt tagtatgagt aatgatgaaa tcctgcgatt tcagaagatg
     4441 gaaaagctct tagaaaataa aatctctcgt cgtttcaaaa tccgcaggtt agataaggac
     4501 gacttcggct atctgattga acacctttac ggacagacag gcactgccta tgaagagtat
     4561 gagtaccatc tatcaaagaa aaagctggat aatgaaacgc tgattaaata ctatgacttg
     4621 attaagccta ctcgctgttt ggtggaagaa aaacagcgat atttgaaaat ccagcaggaa
     4681 gatgaaaccg tctatgtagc ttactttacc attaacagca ttgtcggaga actggacttc
     4741 ccgtcctctg aaatcttcta ctaccagcaa cagcaattta cattcccgat tgatacgtca
     4801 atgaatgtgg aaattgtagc gaatcgtaaa gccctatcta ctgtccgcaa taaaaagaaa
     4861 gaactgaaag acttggataa ccacgcttgg caaagtgata atgaaaccag ctccaatgtg
     4921 gcggaagctc tggaaagtgt gaatgagctg gaaaccaatt tagaccaaag caaggaatct
     4981 atgtacaagc tgtcttatgt ggtaagggta tcagcaaatg atcttgacga actcaaacgt
     5041 cgttgtaatg aagtgaaaga tttttatgac gatttaagcg taaaactggt acgaccattt
     5101 ggggatatgc tcggcttaca tgaagaattt ttacctgcca gcaagcgtta tatgaatgat
     5161 tatattcaat acgtgacctc tgatttcctc gctggtttag gttttggtgc tactcaaatg
     5221 ctgggggaaa atgaggggat ttatgttggc tacagcttag atactggacg caatgtctat
     5281 ctgaaacctg ctcttgccag tcaaggggtt aagggttcag taaccaatgc gttagcgtcg
     5341 gcttttgttg gtt
//
